LOCUS       DAJTXQ010000001        88073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000001 DAJTXQ010000000
VERSION     DAJTXQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.001"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO515_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO515_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6574044.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OO515_000002"
     tRNA            1230..1305
                     /locus_tag="OO515_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO515_000003"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO515_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6574045.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO515_000004"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO515_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6574046.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO515_000005"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO515_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6574047.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO515_000006"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO515_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6574048.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="OO515_000007"
     CDS             complement(4868..5305)
                     /locus_tag="OO515_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6574049.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="OO515_000008"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="OO515_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO515_000009"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO515_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCR6574050.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="OO515_000010"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="OO515_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCR6574051.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="OO515_000011"
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="OO515_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCR6574052.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="OO515_000012"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="OO515_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCR6574053.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="OO515_000013"
     CDS             10773..11441
                     /locus_tag="OO515_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCR6574054.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="OO515_000014"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="OO515_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCR6574055.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="OO515_000015"
     CDS             11984..13816
                     /locus_tag="OO515_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCR6574056.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="OO515_000016"
     CDS             13806..14156
                     /locus_tag="OO515_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCR6574057.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="OO515_000017"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="OO515_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCR6574058.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="OO515_000018"
     CDS             14475..14897
                     /locus_tag="OO515_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCR6574059.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="OO515_000019"
     CDS             14900..15532
                     /locus_tag="OO515_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCR6574060.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="OO515_000020"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="OO515_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCR6574061.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="OO515_000021"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="OO515_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCR6574062.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="OO515_000022"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="OO515_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCR6574063.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="OO515_000023"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="OO515_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="OO515_000024"
     CDS             18711..19823
                     /locus_tag="OO515_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6574064.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19820..21165
                     /locus_tag="OO515_000025"
                     /pseudo
     CDS             19820..21165
                     /locus_tag="OO515_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21168..21722
                     /gene="pduT"
                     /locus_tag="OO515_000026"
     CDS             21168..21722
                     /gene="pduT"
                     /locus_tag="OO515_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCR6574065.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21722..22072
                     /gene="pduU"
                     /locus_tag="OO515_000027"
     CDS             21722..22072
                     /gene="pduU"
                     /locus_tag="OO515_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCR6574066.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22077..22520
                     /locus_tag="OO515_000028"
     CDS             22077..22520
                     /locus_tag="OO515_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCR6574067.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22621..22950)
                     /locus_tag="OO515_000029"
     CDS             complement(22621..22950)
                     /locus_tag="OO515_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6574068.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23122..24180)
                     /locus_tag="OO515_000030"
     CDS             complement(23122..24180)
                     /locus_tag="OO515_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6574069.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO515_000031"
     CDS             complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO515_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR6574070.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO515_000032"
     CDS             complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO515_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR6574071.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26345..27772
                     /gene="sbcB"
                     /locus_tag="OO515_000033"
     CDS             26345..27772
                     /gene="sbcB"
                     /locus_tag="OO515_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6574072.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27815..28042)
                     /locus_tag="OO515_000034"
     CDS             complement(27815..28042)
                     /locus_tag="OO515_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6574073.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO515_000035"
     CDS             complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO515_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6574074.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO515_000036"
     CDS             complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO515_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6574075.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO515_000037"
     CDS             complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO515_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6574076.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30918..31847)
                     /locus_tag="OO515_000038"
     CDS             complement(30918..31847)
                     /locus_tag="OO515_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6574077.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31893..32717)
                     /locus_tag="OO515_000039"
     CDS             complement(31893..32717)
                     /locus_tag="OO515_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6574078.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO515_000040"
     CDS             complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO515_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6574079.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO515_000041"
     CDS             complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO515_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6574080.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33585..33635
                     /gene="hisL"
                     /locus_tag="OO515_000042"
     CDS             33585..33635
                     /gene="hisL"
                     /locus_tag="OO515_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6574081.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33611..33734
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33781..34680
                     /gene="hisG"
                     /locus_tag="OO515_000043"
     CDS             33781..34680
                     /gene="hisG"
                     /locus_tag="OO515_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6574082.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34686..35990
                     /gene="hisD"
                     /locus_tag="OO515_000044"
     CDS             34686..35990
                     /gene="hisD"
                     /locus_tag="OO515_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6574083.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35987..37057
                     /gene="hisC"
                     /locus_tag="OO515_000045"
     CDS             35987..37057
                     /gene="hisC"
                     /locus_tag="OO515_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6574084.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37057..38124
                     /gene="hisB"
                     /locus_tag="OO515_000046"
     CDS             37057..38124
                     /gene="hisB"
                     /locus_tag="OO515_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6574085.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38124..38714
                     /gene="hisH"
                     /locus_tag="OO515_000047"
     CDS             38124..38714
                     /gene="hisH"
                     /locus_tag="OO515_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6574086.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38714..39451
                     /gene="hisA"
                     /locus_tag="OO515_000048"
     CDS             38714..39451
                     /gene="hisA"
                     /locus_tag="OO515_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6574087.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39433..40209
                     /gene="hisF"
                     /locus_tag="OO515_000049"
     CDS             39433..40209
                     /gene="hisF"
                     /locus_tag="OO515_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6574088.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40203..40814
                     /locus_tag="OO515_000050"
     CDS             40203..40814
                     /locus_tag="OO515_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6574089.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO515_000051"
     CDS             complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO515_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6574090.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO515_000052"
     CDS             complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO515_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6574091.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO515_000053"
     CDS             complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO515_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6574092.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO515_000054"
                     /gene_synonym="manB"
     CDS             complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO515_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6574093.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO515_000055"
     CDS             complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO515_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6574094.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO515_000056"
     CDS             complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO515_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6574095.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO515_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO515_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6574096.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO515_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO515_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6574097.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO515_000059"
     CDS             complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO515_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6574098.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO515_000060"
     CDS             complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO515_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6574099.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO515_000061"
     CDS             complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO515_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6574100.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO515_000062"
     CDS             complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO515_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6574101.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO515_000063"
     CDS             complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO515_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6574102.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO515_000064"
     CDS             complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO515_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6574103.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO515_000065"
     CDS             complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO515_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6574104.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO515_000066"
     CDS             complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO515_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6574105.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO515_000067"
     CDS             complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO515_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6574106.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO515_000068"
     CDS             complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO515_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6574107.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60963..61112
                     /locus_tag="OO515_000069"
     CDS             60963..61112
                     /locus_tag="OO515_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574108.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61313..62896
                     /locus_tag="OO515_000070"
     CDS             61313..62896
                     /locus_tag="OO515_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6574109.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO515_000071"
     CDS             complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO515_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6574110.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO515_000072"
     CDS             complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO515_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6574111.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO515_000073"
     CDS             complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO515_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6574112.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66677..69994
                     /locus_tag="OO515_000074"
     CDS             66677..69994
                     /locus_tag="OO515_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6574113.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKMIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO515_000075"
     CDS             complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO515_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6574114.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71086..72438
                     /gene="yegD"
                     /locus_tag="OO515_000076"
     CDS             71086..72438
                     /gene="yegD"
                     /locus_tag="OO515_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6574115.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO515_000077"
     CDS             complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO515_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6574116.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO515_000078"
     CDS             complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO515_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6574117.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75146..75805)
                     /locus_tag="OO515_000079"
     CDS             complement(75146..75805)
                     /locus_tag="OO515_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6574118.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO515_000080"
     CDS             complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO515_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6574119.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76358..76414)
                     /locus_tag="OO515_000081"
     CDS             complement(76358..76414)
                     /locus_tag="OO515_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6574120.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76685..76741)
                     /locus_tag="OO515_000082"
     CDS             complement(76685..76741)
                     /locus_tag="OO515_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6574121.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77020..78261
                     /gene="mdtA"
                     /locus_tag="OO515_000083"
     CDS             77020..78261
                     /gene="mdtA"
                     /locus_tag="OO515_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6574122.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78261..81383
                     /gene="mdtB"
                     /locus_tag="OO515_000084"
     CDS             78261..81383
                     /gene="mdtB"
                     /locus_tag="OO515_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6574123.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81384..84461
                     /gene="mdtC"
                     /locus_tag="OO515_000085"
     CDS             81384..84461
                     /gene="mdtC"
                     /locus_tag="OO515_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6574124.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84462..85877
                     /locus_tag="OO515_000086"
     CDS             84462..85877
                     /locus_tag="OO515_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6574125.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85874..87277
                     /gene="baeS"
                     /locus_tag="OO515_000087"
     CDS             85874..87277
                     /gene="baeS"
                     /locus_tag="OO515_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6574126.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87274..87996
                     /gene="baeR"
                     /locus_tag="OO515_000088"
     CDS             87274..87996
                     /gene="baeR"
                     /locus_tag="OO515_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6574127.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21560 a        23315 c        22145 g        21053 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact accctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac aatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgag agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaagcgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa gacaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccaat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcag ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatattg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcaa ggggttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg cggttaacta ccaacagctc
    20701 gccacgccgc aactgttact gacagcgctc acctgttcag agtgcaacgt ctgcgccagt
    20761 gtcgcctgtc cggtaggaat ttcaccaatg cgcataaacc gcctgcttaa gcaggaatta
    20821 cgcgcacaaa atctccgcta cgaaggtgcc ttaaatcctg cagatcccat ggcaaattat
    20881 cgactgattc ccgtaaaacg gctggtaacc agacttggct taaccccgtg gtatcaggat
    20941 gcgccattgt ctgaacaggt gcctcagccc gaaaaagtga cgttattgtt acgccagcac
    21001 atcggtgcca gcgcaattgc gtgtgtacaa aaaggcgatc gcgtcgttca tgggcaatgt
    21061 gtaggtcaaa ttccacacgg cacattaggt gcccccattc atgccagtat tgatggcatg
    21121 gtcagcgatg tgacagaaaa cgcgattacg cttgtgagag gttaaccatg tcgaaagcgg
    21181 taggtatttt agaacttagc agtatcgcga aagggatgga aaccggcgac gccatgctga
    21241 aaagcgccaa cgttgagtta ctggtcagca aaacgctatg ccccggaaaa tttctgctga
    21301 tgctcggtgg cgatgtgggc gcggtgcaac aggccatcaa cagcggagcg tcccttgctg
    21361 gtgaaatgct ggtcgacagt cttgtcctgc cgaatattca tcccagcctc ctttctgcaa
    21421 tcagcggact taaccatgtt gaacagcacc gggctgtcgg cgtagtcgaa acctggagtg
    21481 tcgccgcctg cattgacgct gccgattgtg ctgtcaaagc tgcaaatgtc attcttgttc
    21541 gcgtacatat ggcttttggc attggcggta aatgctacat ggtggttgct ggcgatattt
    21601 cagatgttaa taacgcggtc tctgccgcca gcgtgcgcgc gggggaaaaa ggtttgttgg
    21661 tctatcggtc ggtgattgcc cgtccacatg aagccatgtg gcgtcagatg gtggagggat
    21721 aatgacaact cagcacacaa ctgaacggat gattcaggaa tatgtcccag gtaagcaggt
    21781 cacgctggca catgtcatcg ccaatccagg gaaggatctg tttaagaaat tgggtttacc
    21841 agacaccatt tccgctattg gtattctgac aattaccccc agcgaagcat ctatcatcgc
    21901 atgcgatatc gccacaaaat caggcgctgt agagatcggt tttcttgatc gttttaccgg
    21961 tgccgtagtg ctgactggtg atgtttctgc gatcgaatat gcacttagcc aggttacccg
    22021 tacgctgggt gaaatgatgc gctttaccgc ctgccccatt acgcggacct gacgttatgc
    22081 aacgtatgat gctcatcggc cccagccagt gcggaaaaac gtcactcacg caatgtctgc
    22141 gtggcgaaaa tattcattat cagaaaacgc aggcaatcgt ctggtccccg accaccattg
    22201 atacaccagg cgaatacctc gaaaaccgca gcctctacag tgcactgttg gtcagtgcct
    22261 gcgaagcgga cattatcgcg ctggtgctta atgcggacgc accgtggtca cctttttcgc
    22321 caggctttac ctcgtcaatg aatcgcccgg tcattggagt agtaaccaaa gcagatttag
    22381 ccaacatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga gcgcacaacg
    22441 tgttagtaac cagtgcggtt aataaccatg gagttgcaga acttttagcc ttgctgcata
    22501 cagaagaagg ctgtcgctaa tttcgacagg ttatcagatg ggaatgatga cggtgataca
    22561 cagaaaagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacaggaa
    22621 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
    22681 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
    22741 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatcccag gcttgatgta
    22801 atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac
    22861 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
    22921 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
    22981 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
    23041 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
    23101 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
    23161 ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
    23221 taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag catcgttcaa
    23281 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaagct
    23341 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
    23401 gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag
    23461 attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
    23521 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
    23581 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
    23641 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
    23701 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
    23761 cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc caatcaataa
    23821 tccttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatcg ccgccgcgca
    23881 ccagactaac atcagcggta acgaaattaa ctccagttgc agagcgataa gacctaaaat
    23941 cgaacccaac accgtaccgc caatacgttc gaaggcacga gggacaacgt taccccagaa
    24001 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
    24061 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
    24121 tatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
    24181 gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc cagaaggcgc
    24241 gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa
    24301 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca
    24361 caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat ttcgatatcc
    24421 cagtacccat cttccgcgcc attgttcaaa taaacctcga agcagggctt tggtaacatt
    24481 tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt agcaaaatca
    24541 tcaccgacta cacgagccac cgccaccgca tactgaccac ctgaaatttc tgtcagaatg
    24601 acgccctcac tgttttcggg aagcgtaaag ttattcggta ccgtcacaac ggtgtcgcag
    24661 cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac ccactccttc
    24721 ggcacaatat ttttgttatc tacccacatc atcaactgct caaagccttt ctttaccgtc
    24781 tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttctcttc ctgcgtaatc
    24841 tcgtagttca tgacgcctcc attaacactg tttttatata cagcatagtt gcaatattaa
    24901 aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca gtctgctgca
    24961 aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca tgccgccttc
    25021 cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac ggtcgtaaag
    25081 ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt taccatcaag
    25141 ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc agaactcttg
    25201 ttccgttcct aggtcgatat tttctttatc gccataccag atgcgttccg ttccgacctt
    25261 tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac gcagtaattt
    25321 tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg caatgagacg
    25381 acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag tatgacccgt
    25441 tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt tttgctgggt
    25501 gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg gctcgccgtg
    25561 gatgatagcg cgagaaaaca cagctaaatc ataagccgag ctatgctggc caggtgcatc
    25621 cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg catagttgtt
    25681 catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag caacacaagc
    25741 gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata cgcgatcgcc
    25801 ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg cccacgcatc
    25861 gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag cgcgatccac
    25921 gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat gctcattacc
    25981 cgcggtgagg atctgaccgg tggtgtaatc catcaatacc caggacccgg catgaatctc
    26041 tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata agttaaaaac
    26101 gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag gagtttcaaa
    26161 tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca agaaaatgtg
    26221 actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa ttgtggcgct
    26281 aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata acggatttaa
    26341 cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt acgaaacctt
    26401 tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca ccgataacga
    26461 attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgctgatg actatttacc
    26521 ccagccagga gccgtattaa ttaccggtat taccccgcag gaagcacggg cgaaaggaga
    26581 aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat
    26641 tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa
    26701 tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg atttgctgga
    26761 tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga
    26821 cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggaattg aacatagcaa
    26881 cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac
    26941 gcgtcagcca cgtctgtttg attatctctt tacccatcgt aataaacaca aactgatggc
    27001 gttgattgat gttccgcaga tgaaacccct ggttcacgtt tccggaatgt ttggggcatg
    27061 gcgcggcaat accagctggg tggcaccgct ggcgtggcat ccagaaaatc gcaatgccgt
    27121 aattatggtg gatttggcag gagacatttc gccattactg gaactggata gcgacacatt
    27181 gcgcgagcgt ttatatactg caaaagccga tcttggcgat aacgccgccg ttccggttaa
    27241 gctggtgcat atcaataaat gtccggtgct ggcccaggcg aatacgctac gcccggaaga
    27301 tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctgaaaa ttctgcgtga
    27361 aaatccgcaa gtgcgcgaaa aagtggtggc gatattcgcg gaagccgaac cgtttacgcc
    27421 ttcagataac gtggatgcac agctttataa cggctttttc agtgacgcag atcgtgcagc
    27481 aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt
    27541 cgataaacgg attgaaaagc tgttgttcaa ttatcgggca cgcaacttcc cggggacgct
    27601 ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca cgcccgagtt
    27661 tttacaaggt tatgcagatg aattgcagat gctggcacaa caatatgccg ataacaaaga
    27721 gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct aatttgaagc
    27781 ctccgccgct ggtacggcgg aggtcaatgt tgagtcaggc tttttgaacg gtgatactcc
    27841 acgctgcatc gccaatctgc tgataatcgg tgatggcatg cccttcttct gccgcccact
    27901 gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg
    27961 ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca
    28021 catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt
    28081 cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc
    28141 ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta
    28201 ccgataaagc aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg
    28261 ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct
    28321 gcaataaatg accccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc
    28381 acgtcacccg cgaccagaaa ttgcagaata ttggccgttg gggaagtgat tcctaaccca
    28441 aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt
    28501 acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccagttcgg
    28561 cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag
    28621 agcgttatca ccagcaaaac ggcaaccaac ggccaaacag acaaattaaa agtctcagca
    28681 atagagttat gttcagtact gtagtgctgc aaggtttgat ttaaaccact ggcatgtgga
    28741 gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat
    28801 ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc
    28861 ccgaagatat acccacctat aacagtacca agccacggga acgcctcggc ttcgtaagtc
    28921 agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat tgcaatcagc
    28981 aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc acctgtcagg
    29041 caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat
    29101 atcattgaaa acataaatta attaaccaga tgaatgttaa tgaggaaatt attcatgact
    29161 ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga
    29221 tttcgataaa aaaaaataaa aaaaccggag ccaaatattc attcgactcc ggttgattat
    29281 tagaaaagat gattatgcaa cgtcttcgta ctgcggaact gggttacgga agctcttggt
    29341 gacgcaagcc aggtaaatca gaccgatagc agcccaaatc agacccagaa ccattgagct
    29401 ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag
    29461 atactggaag tgatctttca gcgtcttatt acgcttctca cggatccaga actgcgagat
    29521 gaccgacagg ttaacgaagg tgaacgccac caacgcacca aagttaatca gcgctgtagc
    29581 cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat
    29641 cgccggagta cgccatttcg ggtgtacata accgaagaag cttttcggga atacgccgtc
    29701 acgacccatt acgtacatca gacgcgctac gcctgcatgc gccgccatac cggacgccag
    29761 tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag
    29821 catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag
    29881 ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat
    29941 cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacaggttgc tgatgccgtc
    30001 aaagccggta aaggaaaagc acaggattgt cgccccggta atcatcggaa taacatgcgc
    30061 atcgccagac cagaacggac gagtgctcgc cagcgtacca gcgccttcac cttcaaatac
    30121 gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac
    30181 agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac
    30241 cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa aatagatttt
    30301 cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac
    30361 cataaagccg acagtcgggc taatggattt ctgggcgtaa gtatatgcag agccagcaga
    30421 aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc
    30481 gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaaagtatc
    30541 aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa ctaacgtaag
    30601 cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggtcattct
    30661 cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag
    30721 cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta
    30781 tgaatgattg gtttgaagca aaaaataacc gacgctgatg aaacgtcggt ttttagtcat
    30841 tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaagctt
    30901 gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc ataaacaccg
    30961 gcgatcgcac tatttgctaa aatttcatcc cgccaccagc cgctggcaag ccctgcggtc
    31021 tgttcgttcc agccaatcca gacgggttca aagagagttt gggcgaccac tttcttctcg
    31081 attaacgcgc cactatcgag aaaacgttgc accagataac ggggtaaata gccgcagccg
    31141 aggccgctaa tttgcagctc cagcttggtt ttaaaatcga aaacggtaat ggcttcttgt
    31201 tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc
    31261 gcccgatatc gcttaatgat acgacggttt aacggctctt cttcctgggc taacggatga
    31321 tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc aaactcactg
    31381 gaagatggtg gctcatgcat cgcgccaaca ataatatccg ctcgcccctg agtcagggca
    31441 tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga
    31501 taaaaagctt caatcagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc
    31561 accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg
    31621 gtatgcagaa cttcccgccc cttctctaat aacatttttc cggtacgggt gaatttagcg
    31681 cggtgaccgc tacgatcaag taattgaata ttaaggtcgc tttccagctt gtgaacggtg
    31741 taactcagag cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa actgccttct
    31801 ttttctaagg catcgaggat catcaacaca tccagcagtt gtttcataca cgcccccttg
    31861 cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac cagcggatcg
    31921 ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct
    31981 ttgccgctgt ccagactatt tctgaactgc ggaggttcca gccccagtaa acgggccatc
    32041 tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg
    32101 cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa
    32161 ttaacaccat gttcaccatc aggcgcggtt tttccggcaa agaagcgtcc gggatgacgt
    32221 cccggtccga ccagccccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc
    32281 agccagtctt cgagttcttc taatactcgt ccactgttgg tcaccggatt acgcggggtg
    32341 gtttctttca ccgtgccttg cgcgtcgcca tagacagacg ttgagctggt aaaaataatg
    32401 cgaggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac
    32461 tcatcgccgg ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccatc
    32521 agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta
    32581 tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact
    32641 tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc ctaaccaccc taacccgaca
    32701 attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag
    32761 cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc
    32821 tgcaatgagc agtgaatcgt cggtaaccgc gtataccaga cggtgctcct ctgtaatgcg
    32881 tcgggaccag aaacctgaca aattatgttt caggggttct ggcttcccct taccttcaaa
    32941 tggcgttctg cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt
    33001 ttcctgccag tacagataat catcccatga ttcctcagac cagattagtt tcactcaata
    33061 atgtcctttt ctgttccttt gcctgatttc aggctatcga ttgagtccat caatctccgg
    33121 gcgttagcgg gggagcgcag tagataagcc gtctcttcca gcgagttgta ttcttcgagt
    33181 gacatcagaa cacaagcctc tccattctga cgagtaataa ggatcggggc atgatcttca
    33241 acggctttca tcattgttgc cgacaaattc tgacgcgctt cgctgtagct aattgtacgc
    33301 atgtcaatct cctcttttgt acagtttatt gtacaatgat gggcgttaat taactattta
    33361 ttaattagtt tgtagatcaa ggtattgtca gtgagacgaa aatccaggct tcgctatttt
    33421 tggtgccgtc agcttagagg acagtcctct tagccccctc ctttccccgc tcattcatta
    33481 aacaaatcca ttgccataaa atatataaaa aagcccttgc tttctaacgt gaaagtggtt
    33541 taggttaaaa gacatcagtt gaataaacat tcacagagac ttttatgaca cgcgttcaat
    33601 ttaaacacca ccatcatcac catcatcctg actagtcttt caggcgatgt gtgctggaag
    33661 acattcagat cttccagtgg tgcatgaacg catgagaaag cccccggaag atcaccttcc
    33721 gggggctttt ttattgcgcg gttgataacg gttcagacag gtttaaagag gaataacaaa
    33781 atgacagaca acactcgttt acgcatagct atgcagaaat ccggccgttt aagtgatgac
    33841 tcacgcgaat tactggcgcg ctgtggcatt aaaattaatc ttcacaccca gcgcctgatc
    33901 gcgatggcag aaaacatgcc gattgatatt ctgcgcgtgc gtgacgacga cattcccggt
    33961 ctggtaatgg atggcgtggt agaccttggg attatcggcg aaaacgtgct ggaagaagag
    34021 ctgcttaacc gccgcgccca gggtgaagat ccgcgctact ttaccttgcg tcgtctggat
    34081 ttcggcggct gccgcctttc gctggcaacg ccggttgatg aagcctggga cggcccgctc
    34141 tccttaaacg gtaaacgtat cgccacctct tatcctcacc tgctcaagcg ttatctcgac
    34201 cagaaaggca tctcttttaa atcctgctta ctgaacggtt ctgttgaagt cgccccgcgt
    34261 gccggactgg cggatgcgat ttgcgatctg gtttccaccg gtgccacgct ggaagcaaac
    34321 ggcctgcgcg aagtcgaagt tatctaccgc tcgaaagcct gccttatcca gcgcgatggt
    34381 gaaatggaag aatccaaaca gcagctgatc gacaaactgc tgacccgtat tcagggtgtg
    34441 atccaggcgc gcgaatcaaa atacatcatg atgcacgcac cgaccgaacg tctggatgaa
    34501 gtcatcgccc tgctgccagg tgccgaacgc ccaaccattc tgccgctggc gggtgaccaa
    34561 caacgcgtag cgatgcacat ggtcagtagc gaaaccctgt tctgggaaac gatggaaaaa
    34621 ctgaaagcgc tgggtgccag ttcaattctg gtcctgccga ttgagaagat gatggagtga
    34681 tcgccatgag ctttaacaca atcattgact ggaatagctg tactgcggag caacaacgcc
    34741 agctgttaat gcgcccggcg atctccgctt ctgaaagcat tacccgcact gttaacgata
    34801 ttctcgataa cgtgaaagca cgcggtgatg acgccctgcg ggaatacagc gcgaagtttg
    34861 ataaaaccac ggttaacgcg ctgaaggtgt ctgctgagga gattgccgcc gctagcgaac
    34921 gcctgagcga cgagctaaaa caggcgatgg cggtggcagt aaaaaatatt gaaaccttcc
    34981 acactgcgca aaaactgccg ccggtagatg tagaaacgca gccaggcgtg cgttgccagc
    35041 aggtcacgcg cccggtcgct tcagttgggt tgtatattcc tggcggctcc gccccgctct
    35101 tctcaacggt attaatgctg gcgactccgg cgcgtattgc gggctgtaaa aaagtggtgc
    35161 tgtgctcacc gccgccgatt gccgatgaga tcctttatgc ggcgcagctg tgcggtgtga
    35221 aggacgtgtt taacgtcggc ggcgcacagg ccattgccgc gctggcgttt ggtacggaat
    35281 ctgtgccgaa agtggacaaa atcttcgggc cgggtaacgc ctttgtcacc gaagcgaaac
    35341 gtcaggtgag ccagcgtctg gacggtgcgg ctatcgatat gcccgcaggc ccgtcggaag
    35401 tgttggtcat tgccgacagc ggcgcaacgc cggatttcgt ggcttctgat ttgctttctc
    35461 aggctgaaca cggcccggac tcacaggtga ttttactgac gcctgacgct gatatggcgc
    35521 atcaagttgc cgaagccgtc gaacgccagt tagcagaact gccgcgtgcc gaaaccgccc
    35581 gccaggcact gaacgccagc cgcctgatcg tgactaaaga tttagcgcag tgcgtagaga
    35641 tctccaacca gtacggcccg gagcacctga tcatccagac ccgcaacgcc cgcgatctgg
    35701 tcgatggcat caccagcgcc ggttcggtat ttcttggtga ctggtcaccg gaatcggcag
    35761 gtgattacgc ctccggcacc aaccacgttc tgccgactta cggttacacc gccacctgtt
    35821 ccagcctcgg gctggcagat ttccagaagc gtatgaccgt gcaggaactg tcgaaagaag
    35881 gtttctccgc gctggcttcg accattgaaa cattggccgc cgccgagcgc ctgaccgccc
    35941 ataaaaatgc cgttactttg cgtgttaacg cccttaagga gcaagcatga gcaccgtgac
    36001 tattaccgat ttagcgcgtg aaaacgtccg caacctgacg ccgtatcagt cggcgcgtcg
    36061 tctgggcggt aatggcgacg tctggctgaa cgccaacgaa taccccactg ccgtggagtt
    36121 tcagcttact cagcaaacgc tcaaccgcta cccggaatgt cagccgaaag cggtgattga
    36181 aaattacgcg caatatgcag gcgtaaaacc ggaacaggtg ctggtcagtc gtggcgcgga
    36241 cgaaggtatt gaactgctga ttcgcgcttt ttgcgaaccg ggtaaagacg ccatcctcta
    36301 ctgcccgcca acgtacggca tgtacagcgt cagcgccgaa acgattggcg tcgagtgccg
    36361 cacagtgccg acgcaggaca actggcaact ggacttacag ggcatttccg acaagctgga
    36421 cggcgtaaaa gtggtctatg tttgcagccc caacaacccg acagggcaac tgatcaatcc
    36481 gcaggatttt cgcactctgc tggagctaac ccgcggaaaa gccattgtgg ttgccgatga
    36541 agcctatatc gagttttgcc cgcaggcatc gctggctggc tggctgacgg aatatccgca
    36601 cctggctatt ttgcgcacac tgtcgaaagc ttttgctctg gcgggccttc gttgcggatt
    36661 tacgctggca aacgaagaag tcatcaacct gctgatgaaa gtgatcgccc cctacccgct
    36721 ctcgacgccg gttgccgaca ttgcggccca ggcgttaagc ccgcagggaa tcgtcgctat
    36781 gcgcgaacga gtgacgcaaa ttattgcaga acgcgaatac ctgattgccg cactgaaaga
    36841 gatcccctgc gtagagcagg ttttcgactc cgcaaccaac tacattctgg cgcgctttaa
    36901 agcctccagc gcagtgttta aatctttgtg ggatcagggc attatcttac gtgatcagaa
    36961 taaacaaccc tctttaagcg gctgcctgcg aattaccgtc ggaacccgtg aagaaagcca
    37021 gcgcgtcatt gacgccttac gtgcggagca agtttgatga gtcagaagta tctttttatc
    37081 gatcgcgatg gaaccctgat tagcgaaccg ccgagtgatt ttcaggtgga ccgttttgac
    37141 aaactcgcct ttgaaccagg cgtgatcccg gaactgctga agctgcaaaa agcgggctac
    37201 aaactggtaa tgatcaccaa tcaggatggt ctgggaacac aaagtttccc gcaggcggat
    37261 ttcgatggcc cgcacaacct gatgatgcag atcttcacct cgcaaggcgt acagtttgat
    37321 gaagtgctga tttgtccgca cctgcccgcc gatgagtgcg actgccgtaa gccgaaagta
    37381 aaactggtgg aacgttatct ggctgagcaa gcgatggatc gcgctaacag ttatgtgatt
    37441 ggcgatcgcg cgaccgacat tcaactggcg gaaaacatgg gcattaatgg tttacgctac
    37501 gaccgcgaaa tcctgaactg gccaatgatt ggcgagcagc tcactaaacg agaccgttac
    37561 gctcacgtag tgcgcaacac caaagagaca cagattgacg ttcaggtgtg gctggatcgc
    37621 gaaggtggca gcaagattaa caccggtgtt ggcttctttg atcacatgct ggatcagatc
    37681 gccacccacg gtggtttccg tatggaaatc aacgtcaaag gcgacctcta tatcgacgat
    37741 caccacaccg tcgaagatac cggcctggcg ctgggcgaag cgttaaaaat ggctcttggc
    37801 gacaaacgcg gtatttgccg ctttggtttt gtgctaccga tggacgaatg ccttgcccgc
    37861 tgcgcgctgg atatctctgg tcgcccgcac ctggaataca aagccgagtt tacctaccag
    37921 cgcgtgggcg atctcagcac cgagatgatc gagcacttct tccgttcgct ctcatacacc
    37981 atgggcgtga cgctacacct gaaaaccaaa ggtaaaaacg atcaccaccg tgtagagagc
    38041 ctgttcaaag cctttggtcg caccctgcgc caagccatcc gcgtggaagg cgacaccctg
    38101 ccctcgtcga aaggagtgct gtaatgaacg tggtgatcct tgataccggc tgcgccaatc
    38161 tgaactcggt gaagtcagcc attgcgcgcc acggttatga accaaaagtc agccgtgacc
    38221 cggacgtggt gttgctggcc gataaactat ttttacccgg cgttggcact gcgcaagcag
    38281 cgatggatca ggtgcgtgag cgcgagctgt ttgatctcat caaagcctgt acccaaccgg
    38341 tactgggcat ctgcttaggg atgcaactgc tgggtcggcg cagcgaagag agcaacggcg
    38401 tcgacttgct gggcatcatt gacgaagacg tgccgaaaat gaccgacttt ggtctgccgc
    38461 tgccgcatat gggctggaat cgcgtttacc cgcaggcagg caaccgcctg tttcagggga
    38521 ttgaagacgg cgcgtacttt tactttgttc acagttacgc aatgccggtc aatccgtgga
    38581 ccattgccca atgtaattac ggcgaaccgt tcaccgcggc ggtacaaaaa gataacttct
    38641 acggcgtgca gttccacccg gagcgttctg gtgccgctgg cgctaagttg ctgaaaaact
    38701 tcctggagat gtgatgatta ttccggcatt agatttaatc gacggcaccg tggtgcgtct
    38761 ccatcagggc gattacggca aacaacgcga ttacggtaac gacccgctgc cgcgcttaca
    38821 ggattacgcc gcgcagggtg ccgaagtgct gcatctggtg gatctgaccg gggcaaaaga
    38881 tccggcaaaa cgtcaaatcc cgctgattaa aaccctggtc gcgggcgtta acgttccggt
    38941 gcaggttggc ggcggtgtgc gtagcgaaga agacgtggcg gcgttactgg aagcgggcgt
    39001 tgcgcgcgtg gtggttggct ccaccgcggt gaaatcacca gaaagggtga aaggctggtt
    39061 tgaacgtttc ggtgccgatg ccttagtgct ggcgctggat gtccgtattg acgagcaagg
    39121 caacaagcag gtggcggtca gcggctggca agagaactcg ggcgtttcac tggaacaact
    39181 ggtggaaacc tatctgcctg tcggcctgaa acatgtgctg tgtaccgata tctcgcgcga
    39241 cggcacgctg gcaggctcta acgtctcttt atatgaagaa gtgtgcgcca gatacccgca
    39301 ggtggcattt cagtcctccg gcggtattgg cgacattaac gatgttgctg ccctgcgagg
    39361 cactggcgtg cgcggcgtaa tagttggtcg tgcattactg gaaggtaaat tcaccgtgaa
    39421 ggaggccatc gcatgctggc aaaacgcata atcccatgtc tcgacgttcg tgatggtcag
    39481 gtggtgaaag gcgtacagtt tcgcaaccat gaaattattg gcgatattgt accgctggca
    39541 aaacgctacg ccgaagaagg tgcagacgaa ctggtgttct acgatatcac cgcttccagc
    39601 gatggccgtg tggtagataa aagctgggta tctcgcgtgg cggaagtgat cgacattccg
    39661 ttctgcgtgg cgggtgggat taagtctctg gaagatgccg cgaaaattct ttcctttggc
    39721 gcggataaaa tttccatcaa ctcccctgcg ctggcagacc caaccttaat tacccgcctg
    39781 gcggatcgct ttggcgtgca atgcattgtg gtcggcattg atacctggta cgacggcgaa
    39841 accggtaaat atcatgtgaa tcaatatacc ggcgatgaaa gccgcacccg cgtcactcag
    39901 tgggaaacac tcgactgggt acaggaagtg caaaaacgtg gagccggaga aatcgtcctc
    39961 aacatgatga atcaggacgg cgtgcgtaac ggttacgatc tcgaacaact gaaaaaagtg
    40021 cgtgaagttt gccacgtccc gctaattgcc tccggtggcg cgggcaccat ggaacacttc
    40081 ctcgaagcct tccgcgatgc cgacgttgac ggcgcgctgg ctgcttccgt attccacaaa
    40141 caaataatca atattggtga attaaaagcg tacctggcaa cacagggcgt ggagatcagg
    40201 atatgttaac agaacaacaa cgccgcgaac tggactggga aaaaaccgac ggactaatgc
    40261 cggtaattgt gcaacacgcg gtatccggcg aagtgttaat gctgggctat atgaacccgg
    40321 aagccttaga caaaaccatc gaaagcggca aagtcacctt cttctcgcgc actaaacagc
    40381 gactgtggac caaaggcgaa acgtcgggca atttcctcaa cgtagtaaat attgcgccgg
    40441 actgcgacaa cgacacgcta ctggtactgg cgaatcctat cggaccaacc tgccacaaag
    40501 gcaccagcag ctgcttcggc gacaccgctc accaatggct gttcctgtat caactggaac
    40561 aactgctcgc cgaacgcaaa tctgccgacc cggaaacctc ctataccgcc aaactgtatg
    40621 ccagcggcac caaacgcatt gcgcagaaag tgggcgaaga aggcgtagaa accgcactgg
    40681 cagcgacggt acatgaccgc tttgagctga ccaacgaggc atcggacttg atgtatcacc
    40741 tgctggtgct gttgcaggat caggatctgg atttaacgac ggtaattgag aacctgcgta
    40801 aacggcatca gtgagttgca gggtaagcgg atgcgatgtt gttgtcgcat ccggcaaaaa
    40861 aaaagggcaa ggtgtcacca ccctgcccgt tttctttaaa gctgcaaaga ttacttcgcg
    40921 ttataattac gcagagcgtt acgccccagc acaatccccg cgccaaccat gccgcccagc
    40981 agcaccgcca gaatcaaggt aattgccttt ttcgggctat cgcgacgaat aggtaacgtc
    41041 ggtttcatca cataacggta agcatgaata tcaagatcat caacctttaa gttttcaata
    41101 tccaacaggt tttgacgtgt ctgatagtag tttggtgaga acaccaacgg acgggtcgcc
    41161 tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa ggaacaacgt atcttgtgtc
    41221 acatcttcag tctgctgaac ctgtggcttt gccacctgcg cctgattcgc atactgcaac
    41281 gcttcctgaa tctgacggat acgcagatct ttctgctcct gcgcgactac ttcctgcgtt
    41341 cttaaagagt cctgcaagtt tttccgtccc agagcaatgt tgtctttgag atccttttct
    41401 agctcttgat tcactttatc atcaacttgc tgaatgtatt gggccaactt catttgtgct
    41461 ccctctgcag tttgcccaac ataagaaaca gtcaatggta attgctggtt cttaacagaa
    41521 ggttcgatgg taagtttttc tggttcttcc tgattatcca gcgtttctgc taatgcagag
    41581 aaggcagaac tgaagcgacc aattaacgtc tcctgtaaat ccgatacttt cggtgcagcc
    41641 tgaccataga taacattcat ggcattgtta tagccagcaa tttgccccac gtcgggctga
    41701 gtgataattg ctgttgacgt ccatttctcc ttcgctactg ccaaatatcc aatagccagg
    41761 gcaatagcca caatgacgga aatgatgatt gtcatcttgc cacgccacaa ctgcactagt
    41821 aaatcaatca aatcaatctg ttccgggtca tggttttgcc cagaaacatt attattttct
    41881 actctcatca ttaccctaac tgacggaaat ttccaagtcc gcatagttta gcgataatcc
    41941 tagtgaatgc tataggataa gtgataaaaa taatgaatta ttggggaggt aacaaaagac
    42001 aatcagggcg taaatagccc tgataacaag atgttagtcg ctgccaaaga gatcgcgggt
    42061 gtatacctta tctgccacat ctttaagctc ttctgccatt cggttagaga taatgacgtc
    42121 ggcttgttgt ttgaagatgg cgagatcacg ttccagccga gagttgaaga atgagtcttc
    42181 tttcatcact ggctcgtaga tgatcacttc aacgcctttc gccttgatac gcttcataat
    42241 cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca gacgataaat
    42301 acccaccact tgcggtttgc gtgacaaaat ggcatcggca ataaaatcct tacgcgtgcg
    42361 gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt tcgccagtaa
    42421 ctgcttggta tctttcggca agcaataacc accataacca aacgacgggt tgttgtaatg
    42481 gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat tcagacctaa
    42541 actttctgcg tagctatcca gttcgttaaa gtatgccacg cgcatcgcta ggtaggtgtt
    42601 tgcaaaaagt ttaatcgctt ctgcttcggt cgaatcggtg aacagggtcg ggatattttg
    42661 cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcgcgttctg aacgctcacc
    42721 gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccttcac ggagaaattc
    42781 cggagagaaa ataatatttt cagtacgata tttcttatgc atcgcttcgg taaaaccaac
    42841 gggaaccgtt gatttgatga ccataaccgc ataaggattt atctcaacta cgtctttaat
    42901 tactgattct acactggatg tattgaaata attagtttta ggatcatagt cggttggagt
    42961 ggcgatgatg acataatcag catcccggta agcttcattt ttatctaatg tggcattaaa
    43021 gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag gagatatccg
    43081 atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct catgattttg
    43141 tgcgattaga agcccgtttg acaagcctac atagccagta ccggaaatgg tgattttcat
    43201 ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc ctgataagag
    43261 ctaaatgttt taacgctacg ttattagctg gtcaacgcta cgaaaaacaa ttcagatttt
    43321 ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt tagtctataa
    43381 gataaatggc aaaaaaaaag cccggtgcaa tatacgccgg gcctcaattt tactgttggt
    43441 taaatcagat taatccagcc attcagtatg gaacacacct tctttatcaa tgcgcttata
    43501 agtatgtgca ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca gaacagcggc
    43561 acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc ggaaccggga taccgttctg
    43621 tactgcataa gcaacgacat cacgcagcgc ctgctggtag tcatcggcaa tttgcttgaa
    43681 gtacggagcc agcagcaggt tagcgatctg cggattttcg gcatatgcat cggtgatttt
    43741 ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga tttcgccgta
    43801 gttcagatcc cagttgtatt cttcagacgc agcacgcagc tgagagaagc cctgagcgta
    43861 agaaacgatt ttgcccagat acagcgcacg gcgaactttt tcgatgaact cagccttgtc
    43921 gcctgctgac tgagcttgcg gaccagagag aactttagat gcggcaacgc gctgatcttt
    43981 cagagaagag atataacgtg caaacacaga ctcggtaatc agcgacagcg gttctccgag
    44041 atccagcgcg ctctggctgg tccatttacc ggtacctttg ttagccgctt catccaggat
    44101 cacatcaacc aggtagttac cgtcttcatc ttttttagtg aagatatctt tggtgatgtc
    44161 gatcaggtag ctgctcagtt caccgttatt ccactcggta aaggtctgtg ccagttcttc
    44221 gttagagaga ttcaggccgc ctttcaggag agaataggct tcagcaatca gttgcatatc
    44281 accgtattca ataccgttgt gaaccatctt cacataatgg cctgcgccat cagcaccgat
    44341 ataggtaacg cacggttcgc catcttcagc aacggcggcg attttggtca ggatcggcgc
    44401 aaccagttca taggcttctt tctggccacc aggcatgatg gacggccctt tcagcgcccc
    44461 ctcttctccg ccggaaacac cggtaccgat gaagttaaag ccttcggcag aaagctcacg
    44521 gttacgacga atggtgtcct ggaagaaggt gttaccacca tcgatgatga tgtcgccttt
    44581 atcgagatat ggtttgagcg aatcaatagc agcatccgtg cctgcacctg ctttcaccat
    44641 taacaggatg cgacgaggcg tttccagaga ttcaacaaac tctttcaccg tatagtaagg
    44701 aaccagtttc ttgcctggat tttcggcaat cacttcttcc gtcttttcac gggaacggtt
    44761 gaaaatagag acggtataac cacggctttc gatgttgagc gcaaggttgc gccccatcac
    44821 tgccataccg actacgccga tctgttgctt tgacattgtt tactcctgtc agggggtggt
    44881 caccgccgag tgtgcggctt aaatgtggta tagatgttaa ctcactcata gagatggtga
    44941 atagcattac ctttgctata ccacactgaa tatctcacct tttccgtctt ttaaaactta
    45001 tttatatgaa attactgaat tacttgttca gtaactcaag aataagtttt gtcttttctt
    45061 ccatcagcgg cacatcaccg cgagattcca cattcaaccg caccaccggt tcggtgttgg
    45121 aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg gtgcgatcca
    45181 ccgccagcgc ctcgcggcta aaatgctgtt ccacgcggtt aatcgcctca aagggttgcg
    45241 ccagtttgct gttgatctca ccgcttgccg gaaacgctgc catccggtcg cgcaccagtt
    45301 cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac gggatcatgc
    45361 cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttca ccaccgtaga
    45421 tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc gacatcaccg
    45481 gcgtgccgcc tgcggcagtc accacatcaa cggtgttcca ggagagacgc ggatcgtgga
    45541 tgatcttcgc gccgggattt ttttcgagga atgcttctgc cagcaggccg acaatgtagt
    45601 agccctcaat aaactgccct ttttcgtcaa acaggaagca gcggtcaaaa tcgccatcaa
    45661 aagcaatgcc catatccgca ccgtgtttga tgaccgcatt gcgggtgtcg tcgcggcatt
    45721 ccggcagtag cgggttagga ataccgttgg ggaaattgcc gtccggcgtg ttgtgcactt
    45781 tgattaattc cacgggtgcg ccaagggctt taaagcgggc ttcgatggcg tccaccaccg
    45841 gacccgctgc gccattcccg gagttaatca ccagcttgag cggcgtgagg tttttgacat
    45901 tgatataacc gaacaggtga tcaacgtaag cgtcacgcag attgatttgc tgatagcgac
    45961 cgcgtttggt ttcatcaacg ggaggaaagt cgttggcttc tgccagacgc tggacatcgc
    46021 gcagtccggt atcaccgctg atcgggcgag ccccttcgcg caccagtttc atgccgttgt
    46081 aatccatcgg gttatggctg gcggtaactt caatgccgcc atccacgccg agatggaacg
    46141 tggcgaaata gatctcttcg gtgccggaca tgccaatatc cagcacatcg acgcccgcat
    46201 cctgtaaacc tctcgccagc gccagtttta aggtttcgct ggtgaggcgg acatcaccgc
    46261 ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg cgccaggcga
    46321 tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta aagcaggtta
    46381 attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa acggccaatt
    46441 ttgtttttgt cgttatttac atctcggttt cgcattcatt gcctgatgcg acgtttacac
    46501 ccgtccgtag cgatccgcga aacgcaccac atcatcctct tcgagataag agccggagcg
    46561 cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcaatgca tcgcccccag
    46621 cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa tggtgacttt
    46681 tgccgttccc gcgacaacca cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa
    46741 gccctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg cgtcgataga
    46801 gtcatatttg ccccacgggc gatagacttc gcgatgcacc cgatgctcat ggcgaccatc
    46861 ggctttgatc tgctcgacca cttttttcac atcctgcacc gcgttacggt cggcaatcag
    46921 caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg ttacgaggcc
    46981 agattcggcg tacacatagc tgttttcagt tttgtgatta atcacatcgc cgtggcaaac
    47041 gttgccctcg gcggtgtggg cgctaatttc ccataatgag gaccaggagc cgacatcgct
    47101 ccagcccgca tccatcggca ccacaacggc atctgccgta cgttccatga ccgcgtaatc
    47161 caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa aatcgagatc
    47221 cggatcgacg gcgctcatcg ctttttcaca ggcatcgaga atatccggac gatatttttt
    47281 cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc agtaatattc
    47341 gccgcttgcc acataggcct gggcggtttc cagattcggt ttttcgacaa actgcgccac
    47401 ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa tatagccata
    47461 accggtttct ggtagatccg gcacaatgcc gaaggtcacc agcttgcccg cttcggcata
    47521 tggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca catgatccgc
    47581 cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg ccagcgccgc
    47641 cagcgcaatg gcaggtgccg tgtttcgccc tgccggttcg agaataatgt tctcggtaag
    47701 tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat tgcaaatcac
    47761 caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca gcatggtgag
    47821 atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg gccataagcg
    47881 gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca taattatccc
    47941 cgaatatcat ttataaattg acgtaacacg ttctctttat cgagtgtgcg ttcggcatat
    48001 tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct gacggatccc cgccaccagc
    48061 gcctcgaccg attccggttc tacgcaaacc gcaatgcccg gaaaggtttc gcaaagctgc
    48121 cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc cagaatattg
    48181 gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac cagatggcaa
    48241 tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcgggaa aaattgcatg
    48301 ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc gccctgcccg
    48361 acaatggcaa aaatcagcgg ttcatcgcgc agacgatcgg cagcttcaat aacgttttcc
    48421 agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt atccggcagg
    48481 tcaagctggt tacgaagggc gtcaacatcg gcatctgcaa catgctgaaa acgggcaatt
    48541 tccgaccagt tggggaagaa gatgacgttt tccgccgcca cgcctttttc gatggcttta
    48601 ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt tatgcagtcc gctacgttcg
    48661 aacgccgttg ccagctgtgc cactttgctg cctttgcctt ttccggcaag gcccagcccc
    48721 agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc agaaagtttc
    48781 gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ccacgccgat aatgcgatcc
    48841 ggcttccagc gacgttgcgc catcagagga aagaaactgc tgacggcaaa actgccgaga
    48901 tgtaacaggc gtttcagggt gctcggctgt tttggcacgt acagcgggca gcgccacacc
    48961 gtggcggccc cctcttctcg tttgtaccgc caggcggaat agttctcgcc cacctgccac
    49021 tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat gaccttgtgc cgccagccat
    49081 tccaccatct cgccggtgta tttgccgata ccggttaact ccggcgagta gttaatgccg
    49141 tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga aaataggcgc
    49201 ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc cagcggtaat
    49261 cgtcatgctg ctcatccggc agtatcagct cttcttcagc gactctgaag cgaaaaccga
    49321 gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa aagtgctgcc
    49381 agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc gccatcgtca
    49441 gccgctcgaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc ggcacaaacc
    49501 agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcaccg cgactgttct
    49561 cgacaataaa gtcgagagag acaagcgggg tggagcgcac taccgtggca aagtcttcct
    49621 gacgtaaaaa catcattacc cccgaaagcg gtcttgattc tcaaggaacc actggtaagt
    49681 gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat gcaggcgcgt
    49741 cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa ccacccgacc
    49801 tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc agtcaacgcc
    49861 cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttc tccagccaga cttcgtgcgc
    49921 cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga attcacgcat
    49981 cggtgtaccg ctgccccata ccaccacgtc cggtgcattc tgtgccgtcg cctcgtggaa
    50041 gcgacgcagc aatgcaggga tcacatgcga attactcggg tggaagttgt catgcgggcc
    50101 atacaggttg gtcggcatga ctgagcggta atcgcgtccg tactggcggt tgtatgattc
    50161 gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt
    50221 gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt agatacagga
    50281 cgatccaaga aacagcagtt tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct
    50341 ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg caacaatgcc
    50401 gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg
    50461 caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg cgtaatacca gttccacatc
    50521 accgcgctgt tcgagctgcc gcctgatggc agaaccgacc atcccacgat ggccagcgat
    50581 aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc gtagccgtga
    50641 gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat ttcagacacc
    50701 atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg
    50761 ccgagcagcg tttcaacttc agccggacgg aagtaacgcg gatcaacggc gataatcaca
    50821 tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct
    50881 tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgccgctgc catttccacg
    50941 aactgacgca cggagtactg aacgccggtc gcgataacga aatcttccgg ctgttcctgt
    51001 tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc acgcagggaa
    51061 tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc gattgcgcgg
    51121 gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt gaagagaatt
    51181 ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca gtaggcgtac
    51241 agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt ctgcggaatt
    51301 tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt tttcttttcc
    51361 agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg
    51421 gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag gttgtacact
    51481 tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact caggtcgcca
    51541 taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg atccacgcgc
    51601 tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc tttttccagc
    51661 agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag agcgactttt
    51721 gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
    51781 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
    51841 tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga catcggttgc
    51901 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
    51961 atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
    52021 ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt aatcacctaa
    52081 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
    52141 ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt gtggcgacca
    52201 ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt tgccgccccg
    52261 gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt atattcagac
    52321 tttgttatat aaggctttcg tctttgaggt agtacgttgg cgtaaatgcc aggataattc
    52381 agcccaaaag ccaggcacat gtaatatttg tcgttggact tttttcgcgt cagcacacaa
    52441 ttccatatta ttggtggtag ataccccacc catggaaaat tcagacacca ggccattgag
    52501 ttttttaaat gcataaccgg ctttatacat tttggctgcc agcgcgtagt cggaagaaac
    52561 tttatattcc aggtcataac gccatttttt caagccggat acagggaaaa atatcgcctg
    52621 atgactggcg ggcaggctgt gataaatata ccagcccggt ttggcgctac gtttaatttt
    52681 atgcccgtcg ccaaaatcca gcagcgcatc gccggtgatc atcatgttat ctttttgcat
    52741 ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa atatcgcccg aattgagaaa
    52801 caacgcgaac ttgccttgtg ccatcgtaat gcctttgttc atggcgtcgt agataccgtt
    52861 atctggctcg ctgacaaagc gtaggttata gataccattg agattttcca gatactcacg
    52921 ggtgccgtca ttggaaccgc catcgacaac aatccattcg aagctgatat cttccgcctg
    52981 cgccagatgc gccagcgagg catgtgtttt gactatccct tcgaggttac gaaacgcgac
    53041 agtgattatg ctaagcaaca tgttcttatt cttacctcgt aatatttaac gcttttcgca
    53101 aaataaacgg acagacgatt aaaaatgcat attccgggct aaatatcgaa ccggtaaaaa
    53161 acagcgatac cggagtaaaa agatataatt gcacgcgaaa attgcggtta tcaccaaaag
    53221 cgttgatcat catttttatc actttcccca tgtaccacag cgataaaaac actgcgaacc
    53281 aggaaaaata aataatcagc agatacaaac cattgtctat ggtttttccg acatccgcac
    53341 cgttaaatat tccgaatgat gcgacgtatt cataaagtga gccaaatctg actacaccgt
    53401 caatatgggt caaagaataa ccgaccatca ccagcggacc gacaatacga taatacgatg
    53461 acgatccttc cgtacctaaa tcgcccagac gggtggaaat ataaggaaac gcgattacca
    53521 caccaacccg gaatacagcc agagaaatca atgccaacgg taactttttc ttaatcgcct
    53581 ctttattcag atattgaaat gcccactcca gcaaataaaa caggataaaa gtcataaccc
    53641 ctgaaaacga tccggataat attatccctg cgagaatcat agcatcggtt ttaggcgttt
    53701 tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa tgccagagcg aaaaatgccg
    53761 gttcgaaata cagtgctgtc gtgcgcttgc cgccgaattt aatgaaattc agtacatagc
    53821 tgttgctgta aatcagatat ttcgaaatcg actccataat actgctgcca ccggtgagga
    53881 taatttgcgc catctccacc gccgccagcg ccaccaccag cccgatcacc agataaaaga
    53941 aacgtaatat cttgcgatgg ttgtgtggcg aaatcgtttt aaagcgaata ctccacacca
    54001 tgccaataat gatcacaata tagacaaaca gcatggttga agtgacgtat ttactggcat
    54061 ccagcgactg accaaacaga tagttaaacg ccgtgagtcc cgcaccaatc cctaaggcaa
    54121 tcatcaattt tttaacgctg atgcgttcta aaaacagcag taacaagaca ggtaaaaaag
    54181 tgacgatggt gatggggaaa ctttcgccaa gctgggcgat tttgacgtta accagcaggt
    54241 agatcagcgg cagcagcagg tagctacaga ttctgataga agttgacata ctcctccagc
    54301 atctgttgtc cactgtaggt ggcgcggctg cgttggctga actcagccag cgtggtacca
    54361 aatatcgcct gcgcgatttc cggtttgctt aactgcacca gttgcagcac gtcttcttcg
    54421 tcgacggttt taccgccgga tttttgcaac acttcccgtg ccgcgtcgct atgggtggca
    54481 atcaccggca cgccaattga taacgcctca cacaaaatca gcgggtagtt atcgacgcga
    54541 gaactgaata ccagcgcatc catctgattg agcgcgctca tcaacttgcg cttgtcagtt
    54601 tcaaagccgt gattaaccac gttgccagcg gtgaacggcg agaacttacc aaaggtatgc
    54661 agttcgattt tgtcgcccag cgccatcatc tcacgcacca gttgctggtt agtcttgccg
    54721 tcgtaacgca agtcatgcgc caccaccgcg attttcggct tgccctgggt ttcgcgcacc
    54781 ggaggcaagt ccgccagaat cgcttcggtt gccatatcaa taccattatt gataatccgg
    54841 caacgccctg gaccgtacag gctattgaaa gcgtcagcca catgctggct gggggaaata
    54901 aactgacagc ccagcgccag catctcacgg aataactggc gtttgcccgc caccagctgg
    54961 tgtgcgcgat caatcttcac cggcggatag ttatttaagg tcgggcattt ctggcagccc
    55021 gttttccagc cttcgcaacc gtcggtaaag gcgcagcgtc cggtaacact ccagtggtcg
    55081 tgcagcgtcc agaccagagt gacgtccggt ttatggtttt tcactttttc gcaaaagcgc
    55141 accacgctct taagatttag ccagtagctg tgcagcacat gaaaatgcag gaccaccgga
    55201 cccggtgtgc gagtaatggt gcgatataac tcattgaaat tgccaaacag atcgcgatta
    55261 aacagacgaa acagggcaat attcgccatc gcggtcatcc gcggcgtatg tttgatgacc
    55321 tgcggatagt tctgatggct gacgctctct ttgccgcctt tgccataacc gtagacaaaa
    55381 tgtgacgcca gcccctgttg cagcgcacgc tggtggagat ctaacgccac acctgctgcc
    55441 ccgccttccg ccagtcgcac attaaattgc agaatattca tttaattacc ttcactcgcg
    55501 ctttttctcc caccaccagc gcgttgtccg ggacagagtc gagcaccacg ctgcccgcac
    55561 ccacggtgac gttgttacca agcgtgatat caccaagaat aatgacgttg gcaccgagtt
    55621 cgacgccgtt gccaatgtgt ggacatgcca tgttatcggc accacgattg ccgatagtga
    55681 cgccgtggcg aatggtgaaa tcatcccccg ctaccacgtt tttattgatc actacggcgt
    55741 aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc ggcctggatt tcataaccga
    55801 aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga
    55861 ggacgttctt tttgcgccac accgaacaaa aatgagcaac acgataggca agaaccatgc
    55921 agcacgggcg taaactccag ctgttggcac gcagatcttc cagcatcttt agcgcccccg
    55981 aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca aagtgcgcca
    56041 ggtcatacgt ttattgcgga tctggtagag ggtaaacagc tgatattttt tgcttgcacg
    56101 gtcaaattta tctttgtgct ttcggtaaaa gtggaagtac ccggagaatt ttttcggtga
    56161 cgaggtgatc tgcatttcac cgtgattgat atgcaggatc tgcgtcgctt cttccacttt
    56221 ccacggttcg ccgtactcca ccaccatccg caggaagatg tcgtaatcct gtgctgcttt
    56281 cagttcggta tcgaacaaac actctttgaa acgccatgcc caagtaaaga cctgattgcc
    56341 aatgatattg cgtttataga acaggcggcg tgagtacggc gatttgggat acagtggtaa
    56401 gcttgccggt tgggaataaa cttctccctg gcatacgtag tcgttagcgt acaaaaaggc
    56461 gtgagtgacc agttgctgtt tatgggcgag gaagacgctc agacggttgg gtgtccattc
    56521 gtcatcgtca tcgatcccgg taatgtattc cccttgcgcc agcataatcg cctggttacg
    56581 taccgcgcac gccccgctgt taatgtcgtt gtgaatgtag gtgatacgcg gatcgttgag
    56641 ggcagtgacg tactgttgca gttgttccca ggaggtggag caatcatcca cgatgatcat
    56701 ctcccagttg ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg
    56761 acggttccag gtcggcatat agattgaaat cagcggattc tctttcatag gttgttctcc
    56821 cggcttatga gctggtttgc ttgccggatg cggcgtgagc gccttatccg gcctacgggg
    56881 cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat attcgtattc gtaatagcca
    56941 taatcctgat acgcgctggc gcggcggaag atggagttca gaatcacccc tttcaccgga
    57001 ataccgtttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt gttgaccgca
    57061 tagcgcgcca ccattaacgt ggttccagcg tgacgcccga caattgctgc atcggtcact
    57121 gccagaatcg gcggcgtatc aatcaacacc aggtcgtagt ttttactcgc ccagctcacc
    57181 aattcggcaa agcgttcgct catcaacagt tcagaaggat ttggcggcac ctgaccgcgc
    57241 gggatcaggt caaatttggc aatagaggtc ggtttagctg cggtagtaat atcgccctgg
    57301 ccaatcagaa tttccgacag gccattgacg ttattggtac ccaacagctc gtgggtgtag
    57361 cctttgcgca tatcgcagtc gatcaacaac acccgtttat tggtctgact gatcaccgcc
    57421 gccaggttgg cgcagacaaa ggttttaccg attgacgggc tcacccctgt catcatcaac
    57481 acattgttct gcgcctgcat catcgcgaag tgcagactgg tacgcagact gcggatggct
    57541 tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt atagcgttta
    57601 atccctttga tggttttgac gctatcgcgc gctttctgcc attccgacag cgggatgctg
    57661 gcatagacgc tgataccatg ttcttccagc acctgcgggc tttcgatgcc gcgattaaac
    57721 aacgagcgca gcagcacacc cacgatagag agcatcaggc cgaggataat tgcgccgagg
    57781 ataatcagtc ctttcttcgg tttcagcacg ccaggctggg tgattgccgg gtcaacaatg
    57841 cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt attcagcagt
    57901 tgcatataga cctgctgacc agactcgaca tcgcgggtca ggcgcacaat ctcctgctgg
    57961 gttttcggca tcgccgttac gcgaccgtta agtttggctt tctcttcttc cagcgcctga
    58021 cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt ggagatttcc
    58081 gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga atcgagcacc
    58141 gctttcgctt ccagcggcag atcaacagaa tctttatcct gacggaaggc attcagtttg
    58201 ttttcggcaa catcgaggcg gctacgtact tccggtaact gttgcgcgag gaaagcgagg
    58261 cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt acgggcgatg
    58321 ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag gctcagtacg
    58381 cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt gcagttggtt gatcatcccc
    58441 aacgtggagt atttggtgac ggtaaactca ctgcccgggc gggcgtgaat ggcttcaacc
    58501 atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccacgggc gctaaagccg
    58561 ccatcactgc tcagggtgta gtttttattg tccagcacat taagcgtaaa cacctggccc
    58621 gccatctctt tcgggcggtt aaaggtagtc actttcaccg tctcgttctg gcgtcccatc
    58681 agtcgctccc agcccgcccc gaaaatcggg aacgtgtttt tgctcactgc aatatcgagg
    58741 tcgagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg gatctcggcg
    58801 tccgatgcag gcggtttgtt ggctaatgcc gaaccaatgt cctgcactaa cgaattgccg
    58861 ctgttttgct cgatttgtac cagtgcgtcg gcactataaa tcggcgttgc gaagaaggtg
    58921 taaaccacgg cacagagggc aaatacagcg gtgatgccaa tcacccacca gcgcgcttca
    58981 atgacggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac cggagcggca
    59041 tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc tgcgcccact
    59101 ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg cttttgcgat
    59161 acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact ttgccgcgca
    59221 tctcaggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc aaaatcaggt
    59281 catagttgcg acacaggcgg cggctgattt gtcgtgcaca gtggccttcc agagacagtt
    59341 gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc agcgcgccga
    59401 gtccagcgga ctccactttc agttccgggt gataacgttg cagtaagcgt tccgccgtcg
    59461 gggaacggca aatattgccg acacagacaa ctaatatgtt gttaaacatg acgattacca
    59521 gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag ttggcaacaa
    59581 ctgattgatc agacggttcc agcgggaaac cggggcggtg gtgacataca ccacgtcata
    59641 aggttgcagg cggaattctg tcgccatcac cagcgacgtg gcatcggaca tatccagctg
    59701 gtagatattg gcaatcttgc cattacgccc gccctcgcct ttcagcggac gaatgacaaa
    59761 gatgccgctg gcgttggagg tggtcatgtc gataccttca gcattaccca gggcttcagt
    59821 cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt cacccatcac
    59881 aaatactttc agatcatcat tacgcggcac gtagagaata tcgccgggat aaagcagtcg
    59941 gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgtt cttcacgacc
    60001 attgtgtgtt agcactacgt tgcgccagtc agcggtgtcg gtcaggccac ctgcggcgtt
    60061 gatggcgtcg agaatagtca gtggcacgtt ggtgatcgcc tgttgaccgg atttattcac
    60121 ctgaccggag atataggcct tttgtgagcg gaaggcggcg atattaacgt ccacctgcgg
    60181 gtcagcgatg tacgtcgcta agcgcccggt aatatcactg cggatttcag cgagcgtttt
    60241 cccgactacg tgaaccttgc cgatatacgg gtaaaacata gtgccgtcag gctgtaccca
    60301 gttgccggtg tcgctggagc tgcggtactg accggctggc gtggtgagtt ccgggtgatc
    60361 ccagacggtg acattcagaa cgtcgcccgg cccgacgcga tactggtaat tcgcgatctc
    60421 gctttccagc gtcatattgg ggcgcgctac attcgggcgt gggcgtaatt ggtcaatcag
    60481 gcgcggggtc agcggataaa cattcaccat tttgtcgaga tcgaaatcag cgtcctgctg
    60541 tttgatgacg tctttgccca tcgtcgacat attgctgccc ggaagtactg tgcaaccgct
    60601 tatcaaggtt actgacacca ataatggcat caatttcatt ttggatttca tcattgttta
    60661 tttatcactt tggcagagta attatcctgt gcactattaa tagcaatatc gccatgcaca
    60721 tttaccttgc agttaattga ataaaaattt aactggcatc agtcctaaaa aaattgattt
    60781 catccgcagg ctattgacag aataattcag actggtcttt caggcatcca gacacgctac
    60841 cgcccctggc tttttagcca ccaacacact gatttagctt aatttttcaa accctctctg
    60901 catacaatcg ttgatgagaa agggttattg cggaaattaa cttccgaata taaggtgaca
    60961 ttatggtaat tgaatattgg ctctccaata atgcaggagg aagtgttaca gctaacggaa
    61021 tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa gaattatatt aagtgtcatt
    61081 caatatggtt tttaggagtt tctttaggtt gacaatattt aatattgcgc ctccacatgc
    61141 gatatttctt aaataatgtt ttattattac cacttttaat tcagggataa tgtgaggtta
    61201 ttaccctcaa ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat
    61261 ccatgatcga attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg
    61321 gattgccgat ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct
    61381 cggcattgat aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaggcagcg
    61441 cgaccgcgca cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc
    61501 gtcaatctcc tggctggtca ccctgactca accgctgttc agcttccgct cgtttacctt
    61561 tagcgcccgc gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat
    61621 ggagctgaac gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc
    61681 gaagttctgg ggcgtggtga cgcaaattgt ggtactggac gccattttct cactcgactc
    61741 agtgattacc gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat
    61801 cgctatcagc ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc
    61861 gacgatcgtt attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga
    61921 aggtttcggc ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat
    61981 gatcgaggcg ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca
    62041 gacgctgcgc cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga
    62101 tgcggaactg gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt
    62161 taatccgcag gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtaccgtcag
    62221 cagcattatg acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga
    62281 aatccgccag ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga
    62341 cgcagaagat ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt ctctgcgcgg
    62401 cgaaccgctc aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc
    62461 gttgttacct gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga
    62521 tgagtttggc tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgtcgg
    62581 taacttaccg aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga
    62641 cggttcctgg acggcgaatg gtcatatgcc gctggaagat ctggtgcaat atgtgccgct
    62701 gccgctggat gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca
    62761 gcgtattcca aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca
    62821 ggtggaaagc catcgcgtgc agaaggtgca gattattccg ctgcataagg atggcgagtt
    62881 ggagtacgag gtgtgatggt atcggtcatt ttacccaata gccgaaagga ataagatgag
    62941 ggaaccgcgc cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg
    63001 agccaccctg gcacgttcac cggcttgagc gttccatagt agcgaggaac atcaagtgaa
    63061 cggaacagcc ttcgatctta catattcaga atataatgat gccgccacga acgcctgatg
    63121 cgacgcttta cgcgtcttat caggcctaca ggactggtca cctggtaacc aacatcttct
    63181 ccagcaactt cttcacatcc ttgccattgc gggaatcttt attccgctcg gcccagtcat
    63241 tcaggcgacg tttcgcttcg tcctgtagat gtttgcgcag taactgatcc acttgcaggc
    63301 tgtaattgag ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttcagga
    63361 aatcaatcag tttactttcc ccgttccagc cacccacgac ccgaatatca aactgggtat
    63421 cgcaggtttg atctgccaga ttcaacatgc cttcccctgt cagcgccagc acaggcgatt
    63481 gaccttgcat gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc
    63541 ggtcaagccg cgtcacgtta tcaaagtttt cagcggcctt cacatcaccg ccattacgct
    63601 ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt
    63661 cgacatgtgc ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg
    63721 agaaatcacc agccagtgac atttttccgg tcaacgaaat cggatagtta aacgccttca
    63781 gaatggtacc aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg
    63841 atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc gttaagtttg ccctgcagtt
    63901 gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct gtaaaattca
    63961 ttccgcgcca gcgcacgtta ctggcctgca acaaaatatc agccgtaaag ccttgcagtc
    64021 cctgataggc cggttcatca atacgcgaag aaatgaccgg gcgcggcaac gttgattggc
    64081 tctgcccctg ctgcgcggca ccgttttcac cattcgcggt ttcattaagc gggatgaggt
    64141 tgtcaagatt cagttgcggg aattgcagtc tcagctgcca ttccggtttc tctgtcagcg
    64201 tgacctgtgc ttgcccgctc agcgtactgt cattggcggt caagctgatt tggttaaatg
    64261 aaaggcgttt atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt
    64321 gttttggtaa atcggcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat
    64381 catgcggata atcagaagca tccaccgtac cgttaaggga tattgtgaga tcgcgctgat
    64441 cgcgattaac ccgcccggag aactcaaatg agccacgatg ttggggatct tgttccattt
    64501 gcaggcggat attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc
    64561 tgtccgccac cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg
    64621 gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg
    64681 gcgtcagttg gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca
    64741 gtagtggtaa aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg
    64801 cgccctgggc ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc
    64861 aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca
    64921 tatagtcgcg gaaatcattc ggattcacca gcaacactaa cacagataac ccggccacca
    64981 gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc
    65041 tgaattagtc tttatcgatt cggctggcta ccgcgccctg ctggttgcga tatttcgcat
    65101 cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac
    65161 caattaacat gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca
    65221 caatgcaacc agaccagccc ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca
    65281 gacgcgccaa agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg
    65341 actccagcgt caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt
    65401 cgagaacgat ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac
    65461 cgctcagatc gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaggc
    65521 gtacatccac cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac
    65581 ggccttcatc aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt
    65641 tcgcgcatca ctcccttaac gccaattacg tcaagggcat accaggttat cactgaacgg
    65701 tacacaattt atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc
    65761 gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca
    65821 atgaattgca ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt
    65881 gaacgcccac gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg
    65941 gtatcaacga aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa
    66001 atgccttcga gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgta
    66061 tgttcaacat agctgtaaac cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa
    66121 tgctcaagca gcaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt
    66181 tcttccatcg acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg
    66241 tgttcatcac cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg
    66301 ccggaagccg atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata
    66361 tatttagcga cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc
    66421 aattataggg atttcatccg tctgatacca gtcgaatagc gttgccgcgc gctcagagtt
    66481 aattgttgac aaagaattcc cggtggcaaa ttacgttgat cagttttatg taaggtaaaa
    66541 aatgttatac gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat
    66601 ataaaatgta aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct
    66661 tgcggataaa gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc
    66721 gctgcttcac ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga
    66781 gctttcgcag tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt
    66841 ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg
    66901 aaatatcgcc gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac
    66961 catcaatatt gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta
    67021 taaccccttg caaaatctgg ctgactggct gcgtctggca ctcggcagcg ccattgttcc
    67081 gcctctgttg gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc
    67141 atttttgata tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt
    67201 gttatttaaa ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct
    67261 gctcacgtta gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc
    67321 ttttactttc attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc
    67381 ctttttgatc ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc
    67441 cctgcttgct acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct
    67501 gatcctgctg cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa
    67561 acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg gaatattccg ctatcggtat
    67621 ggcgttagtg ggcaccgagg gacaatggct gcaaaccaac aaagcgctct gccagtttct
    67681 cgggtacagt caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga
    67741 tctcaataaa gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc
    67801 aatggaaaaa cgctactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc
    67861 actggtgcgc cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa
    67921 cgagctaaaa cgcaccgaac aggtcaatca gcaactgatg gagcgcatca cgctggctaa
    67981 cgaagcgggc gggattggca tctgggagag ggagttgaag ccgaatgttt ttagctggga
    68041 taagcggatg ttcgagctgt atgaaattcc tccacatatc aaaccgaact ggcaggtgtg
    68101 gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa aaaatgattc gtgattcgtt
    68161 gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca
    68221 tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gtctcctcgg
    68281 catcaatatg gatatgaccg aagtgaaaca gcttaacgag gcattgtttc aggaaaaaga
    68341 gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg atatggcgat
    68401 gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc
    68461 gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat
    68521 ggagaacatt tacagtgccg acacctcacg ttccgcaatc gaacaagatg tggtgttgca
    68581 ctgtcggagc ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga
    68641 cggcagcaat attggttcgg ttctggtgat tcaggacgtc accgaatcac gcaaaatgct
    68701 gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca accgcgccag
    68761 ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc
    68821 cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc
    68881 gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga
    68941 cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga
    69001 aagcgctcgt tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg
    69061 ggaaggccgg gtacatcggg taggtgccag cgccgggatt accttgatcg atgacaacaa
    69121 tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg
    69181 tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc
    69241 ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatcgc
    69301 ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcgcttaa
    69361 gctctggagt tgcgaaggcg aaattattga tgaacaaaca tttcgtcgta gcttcagcga
    69421 tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc agcaggccgc
    69481 caaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag
    69541 tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg
    69601 gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa
    69661 actgcagctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt
    69721 caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa
    69781 cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg
    69841 actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc
    69901 tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt
    69961 gctggtgaat agcagttatt tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc
    70021 gacgctaacg cgtcttatca tgcctacaaa tcgctcattc cccagaccgg ataaggcgct
    70081 cgcaccgcat ccggcgacca acgtcatgct tcgtctggtt gccagccttc cgtataccag
    70141 atatgcaaca gcgcataaga acgccagggc ttccagcgct cggcataacg gcggatttgt
    70201 gccggtgtca ttcccggaaa tcgccgttta atcagataat catccggcag aaaaacatct
    70261 ttcgcctgcc agccacgcaa agcaaaataa ttcgccgtcc agcgcccgat acccggaaaa
    70321 gtttgcagcg ttttcatcgc ctgctccaca tcgcccggaa ttgtcattgg taagctgccc
    70381 tccagcgccg catttgccag atgaatcagt gcctctgccc gtttcaacgg catgcctaac
    70441 gcttttaatg cctgcgggtc ggctgctgcc agccgctgag gcgtggggaa gcagatatac
    70501 tccggaaaat catccagccg ttcgccataa agctgtacca ctttggcagt caattttgcc
    70561 gccatcgcca cgctcaccag ttggcctaaa atcgcccgca caccctgctc aaaagcatca
    70621 atacagccgg gtaaacgcaa tccgggccgc cccgcgccta actttcccaa cgcaccgtta
    70681 acaatctgcg ggttacattg cagatcaagc aggcggctca ttttcgccag acactctgcg
    70741 gcaacaggtt ctaaacctgc acttaaattt atgtgcagag tatggcgggc tatatccgga
    70801 atagcagtca ctacgccgcg atattcgccc accgccagac tacgggcata ataattgtcc
    70861 gcgaccgttt ccacaccgct caccgcacgg gcggcgagaa atcccaacat ccacgaccag
    70921 tcatacggcg gctgccagtt cagggtatac atcgcatctc cttgttaatc cgctttcagc
    70981 ataaacgtta ttcagacgtt acgctttgct ttcatattcc ggttgtcgcg acagcaacat
    71041 ttcgctaaag tcacgcccct tcttcaccgg catggggatt aattcgtgtt tattggtttt
    71101 gattacggta cagcaaactg ttcagtggcg gtcatgcgtg acggtaaacc gcaattactg
    71161 aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaatcg
    71221 gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg
    71281 ctgttacgtc gggcgattcg ttataaccgc gaagaagata tcgatgttac ggcgaaaagc
    71341 gtgcagttcg gtctttcctc actggcacag tacattgatg atccagaaga agtgtggttt
    71401 gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg
    71461 tttgaggatc tggtctgcgc aatgatgttg cacattcgcc agcaggcgca ggcgcagctg
    71521 ccagaagcga ttactcaggc ggtgattggt cgcccgatca acttccaggg gctgggcggc
    71581 gatgaagcaa acgcccaggc gcaagagatt ctggaacgcg cggcgaagcg tgccggattc
    71641 agggacgtgg tattccagta cgagccgatc gcggctgggc tggattacga agccaccttg
    71701 caggaagaaa aacgggtgct ggtggtggat atcggcggtg gtacgactga ctgttcattg
    71761 ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg aagccagtct gctgggtcac
    71821 agtggttgtc gtattggcgg taacgatctg gatatcgccc tggcgtttaa aaacctgatg
    71881 ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg ccctgccgat cctgccgtgg
    71941 tggaatgcgg ttgccatcaa cgacgtacct gcgcagagtg atttctacag tagtgccaac
    72001 ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta
    72061 cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc gtagcgcaga agagtgcaaa
    72121 attgctcttt caagcgtagc ggaaacccgc gcctcactgc cgtttatcag caatgaactg
    72181 gctacgctga ttagccagcg agggctggaa agcgccctca gccagccact ggcgcggatt
    72241 ctggaacagg tgcaactggc cctggataac gcccaggaaa aaccggacgt tatctacctg
    72301 accggcggta gcgcccgttc tccgcagatt aaaaaagcgc tggcagaaca gttgccgggc
    72361 attccgattg caggcggcga tgactttggc tccgtcaccg ccgggctggc acgctgggcg
    72421 gaagtggtgt ttcgttaatt gaaaaatggc tcagcagcgg cttaaatctg cctgcgcctg
    72481 agccagtttt tctgctgcct gacgtaacgg ttccatcaac ggcatggtcc gtgctggagc
    72541 atgaaggcgg aatcgctgca actctgtcgc gccgactgtc aacgccgatt ccagccgatg
    72601 tcgtttggcg gtcattttcg ccattaccgc ttgtctgtct gctggcgtta tcgcttcatt
    72661 cggcctgaac acaaaacggc gttcacagtt cgctttccag tcgataaccg cctgggtcag
    72721 atggtcgcca aaccctttca cttgcttaac gccacgacgg gtaacatctg cggctgtttc
    72781 aataccaaaa gaccgtaacg ccgctttacg cgcagggcca acgccgggaa tagaggcaac
    72841 atcaataaaa aatccctcca gaaacttctg cttctgccgt tcccttgcag tgtcatgaag
    72901 tgctgccaga gcgcgtttct cttcttccgg taacccgaga atttcgtcct tcattttttc
    72961 gagcatcgtc cgtttggcga taaaaccttc caggccgcct acctgctgga tctggcggac
    73021 taaatggtca tagtccattt tcgcacgatt aaatgcctca cggcgttgct gaacttctgc
    73081 tttgtacgct ttgcttgtca aactgccaat aatccagata gccgccagca caggaaccaa
    73141 aataatataa cgcggttctg cctgaaggcc gcaaagcagc agcgataatg ctgacagtgc
    73201 gatctcaagc agaatgatgt attcgcgccg taacaggcct aaaggaagcg gcctgccagt
    73261 cggttggaaa tgatcgggta atggcagttg caatgctggc ggtgctactg acgccatcac
    73321 catcgcccag acttttgcca gcacaaaatc cccgccggtg gtaatgacct cttcgccgag
    73381 atcaataaaa tagataacac cttgattatc cagcgcacac catgggcagt cagccaaatg
    73441 accgggataa acatgcattg ccgaaacggt acatttcttt aactgttggc gtagagaatc
    73501 cagtgctgat acccacgctt tagccgtcgg acgcccggtt gccacgccac tttccgtaaa
    73561 cgcctgctga aacatggctt caacatcact cggtaacatc gatagcggaa tcgatcgtgg
    73621 cggcggtttt aaaccacgtc gctgattatc tgacgcgtag gcataacgga aatgggtaat
    73681 atccgtctcc agcgcattac ccgcatcaga gataagcggc acaccagaat aaggatgccg
    73741 gccaccaaac aagacgtgaa aaatcagcaa cgcaaggcca aaattatcgt gattttctgt
    73801 gcgttcaaag ccgacaaatg acgacaaggt ttgtagctct ggcggcgtaa aatgcgacac
    73861 gccgacttcg cataaatgca gtgtgccatt ggcgttaatc tgaaaggagt cgctatcaat
    73921 caacaccact ttgctgtcgc gacctaccat aaaactgttc tggtttacgt cacccacgac
    73981 gtgcccgtgc tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa catagagtag
    74041 aaaatcccac gcacaatgag ggtaacgttg acgacgatgt gccgggctat agatcatatg
    74101 aatcggttct ttaccagaaa cttttggcat cataaaaccg ataacttttc cgcctcgtcc
    74161 accgtgaagc gttgcctgcg gccaggcgac ataattcaac aattgcgcgt cagctgtcgc
    74221 agccataaag gcaagtttgt cctgttttaa ggcgggtggc ggcgtgtgat aaatcttggc
    74281 gacgctatcg acaaactcat tgatatcata aaccgcgcct tcaccgcctt tgcccagttc
    74341 acggcctggc gtaacgcact caccagtagc agtatataaa gtgggtttca tggtaaccac
    74401 agcgccagtg ccaacgtttt atcatcatcc gtacgctcgt ttaccgccgg gctggacaaa
    74461 aactgcttta acaattcagg taataaatcg agttgttcct gcgttgctga cgccagtcca
    74521 ttgaaaaacg gggtaaagaa aggcacatga ggagaattat ccagcatatt taacgccagc
    74581 cgctgaatac catccgtaaa tgctgcaact ttatgcgcac gcccagtgct ggtgaaagtt
    74641 tccagtctgg aaacggcatc ttcgtcggta atgaagtgcg tcatgttggc atattcaccc
    74701 accatcggcg tgagaggcaa ttgcagaccg tgacccaaat ctaccaccac gccgccatcg
    74761 ccaatctgca taatcaaggt gccgtcaggc gatgaaatca atcccagaaa cgtgcaggca
    74821 aaatcgcgaa ctgctaatgc tttggcttct gcctcggcaa aaagtcgttg acgaatagtc
    74881 agtaccatat ttgtggcgag gacatcatta agccccaatt caccgtcctg tactttttgc
    74941 accataaacg ccatcgcttc attgacggcg agcgtcgcgc cttctcctcc ctgcgaaacg
    75001 ctacctgcgc catcagcaag gaacatcacc agtaatggtt gctggtcgtt taaccagaca
    75061 acctgcatct ggcaggcatc ctgacaaggc aaatcagcac taatgtgcga tgttccaaca
    75121 gcggatgcat aaaccagacg ccagctcaca ctgacgtcca gccttttggc gcttccagca
    75181 caacttccgt tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat aaccagctaa
    75241 acagttcacg gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg atttgtgcca
    75301 gagtcttcat atccgccccc tgaacaccga tggaaaagaa ggcaaacttc ttatcttctt
    75361 ccccctgaaa cactttgttg gcggcagcct gccactcatc ggttggagcg ccatcggtga
    75421 tcaggaaaat ccacgggcga taatacgaaa taccatttgc gcggtactca cgtttcctct
    75481 cttcaaccat atccagggct ttggtaatgg cggcccccat aggcgtatcc ccctgggcaa
    75541 acaggatggg cgggaaaaag ttagcggcgc tggtaaaggg ttgttccaca tgcaccgggc
    75601 cgaacgtcac aatgccgagt tccactcttt ttaaagccag cggatcagca agcagttcat
    75661 cacgaaaggt aaccaatccg gcgttaagtt cattaatagg tctgccgctc atcgagccag
    75721 aaacatccag cagcaaaatg caggggcaac gtggttccgg gttactggca aaatcgctgg
    75781 tggcaaacgt gatttgttcg ctcataagaa atcctttctt agagaattat cgaggtccgg
    75841 tatcataccg attgcaataa tgaaataaga gcaaacgaag aaactttcag aattaggccc
    75901 gtcggcgcag aaaattttgc tattcgcctc gcaagaacgg agagccgtcg attgacacta
    75961 agggcggagt gacataattt caggagtgag ggttagggag aggtttcccc ctccccctgg
    76021 tgttcttagt aagcctggaa gctaatcact aagagtatca ccagtatgat gacgtgcttc
    76081 atcataaccc tttccttatt aaaagccctc ttctccggga gagggtttcc cgtttcagcg
    76141 tcccgctgaa atcgtcggct tacctccttt cgccctgaat gcagtctatc gctaacgcgt
    76201 gaacaaaata gtttcatctg tgattattgc gtaacgactc gcaaagaata tttgctggca
    76261 ggatcgcaga ctacaaagcc tgcggattga caatcttatc gtgaaggcat aatttcagga
    76321 gtgagggtag agcggggttt cccccgccct ggtagtctta gtaagcgggg aagcttatga
    76381 ctaagagcac cacgatgatg agtagcttca tcatgaccct ttccttatta aggccccttc
    76441 ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc
    76501 cctgtatgca gtctatcgct gacttgcgga taaaatagtt tccagtgtga ttattgcgta
    76561 acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc agattgacaa
    76621 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    76681 agccttagta agctggccag ctaatgacta agagcaccac gatgatgagt agcttcatca
    76741 tgaccctttc cttatttatg gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    76801 ctgaaatcgg cggcttacct cctttcgcca tgcaagcagt ctatcgctaa cgcgtagata
    76861 aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgtc attcagacga
    76921 ttcccgacag tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta
    76981 tcattccgcg aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc
    77041 cgttgggtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    77101 aatgactccc agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    77161 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    77221 caggcagttc cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg
    77281 gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc
    77341 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    77401 acccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
    77461 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
    77521 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    77581 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    77641 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    77701 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    77761 caggcgcaaa aagccggaaa aacgctggtg gtagaagcct gggatcgcac caattcgaag
    77821 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
    77881 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    77941 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
    78001 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
    78061 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    78121 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    78181 gtggtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
    78241 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    78301 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    78361 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    78421 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    78481 cgccgctcga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    78541 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    78601 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    78661 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    78721 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    78781 aaatctcgca gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg
    78841 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    78901 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    78961 gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    79021 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
    79081 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    79141 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
    79201 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
    79261 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    79321 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
    79381 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
    79441 ataacctgac actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
    79501 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    79561 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
    79621 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    79681 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    79741 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    79801 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
    79861 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt
    79921 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt
    79981 tgcaggcacc gcaatccagc tcctttacca atatggccca gcgacaacgc caggtcgcgg
    80041 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
    80101 ctaacccgtc gctgaatagt gcacgtttac aaatcaacct caaaccgttg gatgaacgtg
    80161 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
    80221 tcgatctctt cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc
    80281 agtaccagtt taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt
    80341 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggacc
    80401 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    80461 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
    80521 atactcaggc taaccagtat cgcgtagtgc tggagcacaa caccgaaatt accccgggcc
    80581 tcgcggcgct ggataccatt cgcctgacca gtagcgacgg cggcgtggtg ccgttaagct
    80641 caattgccaa agttgaacag cgttttgcgc cgctttccat caaccatctg gatcagttcc
    80701 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    80761 agattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg caattccagg
    80821 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    80881 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    80941 ttctctcgac gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta
    81001 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga
    81061 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    81121 gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
    81181 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    81241 gtccgttagg tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca
    81301 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    81361 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    81421 cgacgatttt actgtcggtc gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    81481 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    81541 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcgcttgggc
    81601 gcattgccgg ggtcagcgaa atgacctcca gcagttcgct cggcagcaca cgtattattt
    81661 tacagtttga ttttgaccgg gacatcaacg gcgcagcgcg tgatgtgcag gcggcgatca
    81721 acgctgcaca aagtttgctg cccagtggga tgcccagccg tccgacctat cgcaaagcga
    81781 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    81841 aactgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg
    81901 gtgatgtcga tgtcggaggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg
    81961 cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac cgccatcagc aatgccaacg
    82021 tgcgtaaacc gcagggcgcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    82081 atgagctaaa aaccgccgct gaatatcagc cgttgattat tcactacaac aacggcggcg
    82141 cggttcgtct gggcgatgtg gcgacggtga ccgactcagt gcaggatgtg cgcaacgccg
    82201 ggatgaccaa cgccaaaccg gctattttac tgatgatccg caaactgccg gaagccaata
    82261 ttatccagac ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    82321 cggcgattga tctgcaaatt gcccaggatc gctcccccac cattcgcgcc tcgctggaag
    82381 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    82441 tgcgctcggg tcgcgccact attattcccg ccgtttcggt gccggtttcg ctgattggta
    82501 cgtttgcggc gatgtacctg tgcggattca gtctcaataa cctttcgtta atggcgctca
    82561 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcacgtc
    82621 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    82681 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    82741 gcggattgcc gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta
    82801 tttcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    82861 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    82921 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    82981 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    83041 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    83101 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    83161 ataatgtcac cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc
    83221 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    83281 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    83341 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    83401 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    83461 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    83521 tggcacggct gggaatcgac gtgcaggccg ccaacagcct gttaaataac gccttcggtc
    83581 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    83641 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    83701 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaacgct ccgctgtcgg
    83761 tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg accggaaaat
    83821 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    83881 cggtgcgcgg cagttttgcc ggaacagcgc aggtgttcca ggagacgatg aactcacagg
    83941 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    84001 gttacgtaca cccactgacg attctctcca ccctgccctc ggcgggcgtt ggtgcgctgc
    84061 tggcgctgga gctgttcaat gccccgttca gcctaatagc cctgataggg atcatgctat
    84121 taatcggcat cgtaaagaaa aacgccatta tgatggtcga ttttgcgctc gaagcccaac
    84181 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    84241 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    84301 gtgacggctc ggagctgcgg cacctcctgg ggatcaccat tgtcggtgga ctggtaatga
    84361 gccagcttct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    84421 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagaa cttcccgaca
    84481 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    84541 ccatcgttaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    84601 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    84661 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    84721 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    84781 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    84841 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    84901 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    84961 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga
    85021 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    85081 tggctgtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    85141 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    85201 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    85261 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    85321 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    85381 tacttggcag catgggaatg aagcgaattg tggttcaggt ggtgaatcgc tttggttatc
    85441 gtcgggtact ggtagcgacc acgctgggtc tgtcgctgat caccctgttg tttatgacta
    85501 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    85561 actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    85621 cgagcagcgg caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    85681 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    85741 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
    85801 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    85861 aaaggagcgc gcaatgaagt tctggcgacc tggtattacc ggcaaactgt ttctggcgat
    85921 tttcgccacc tgcattgttt tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    85981 gcgtggcttt attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga
    86041 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    86101 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    86161 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    86221 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    86281 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    86341 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgccc tggcaacgtt
    86401 actcgcggca cttgccactt ttctgctggc gcgcggttta ctggcaccgg taaaacgact
    86461 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    86521 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cactggagaa
    86581 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    86641 agcggtgctg cgcggtgaac tggaagctat tcaggatggc gtgcgcaaat tcacgccgga
    86701 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatctcca
    86761 tcagttgtcg atgtctgatg aaggcgccct cgcctatcaa aaagcaccgg tagatttgat
    86821 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa
    86881 actgcaattt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    86941 gttattcaat aatttacttg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    87001 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    87061 tgtcagtgac gagcagctac aaaaattatt tgaacgtttt tatcgcaccg aaggttcccg
    87121 caaccgcgcc agcggtggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    87181 acataatggc cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    87241 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    87301 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    87361 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    87421 gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc
    87481 ttgacgctgt gccgggaaat tcgtcgtttt tctgacattc ctatcgtgat ggtgacggca
    87541 aaaatcgaag agatcgaccg cctgctcgga ctggagattg gcgcagatga ctacatctgc
    87601 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    87661 ccgcagcgcg aattgcagca acaggatgcc gaaagcccgt tgattatcga cgaaggtcgt
    87721 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgttg
    87781 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    87841 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaacctgcgc
    87901 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    87961 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc
    88021 cggagatggc gttcagacca gatccggcaa cattatccca cgcatggtca gca
//
LOCUS       DAJTXQ010000002        79333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000002 DAJTXQ010000000
VERSION     DAJTXQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.002"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            29..951
                     /locus_tag="OO515_000089"
                     /pseudo
     CDS             29..951
                     /locus_tag="OO515_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            1156..1887
                     /gene="bluR"
                     /locus_tag="OO515_000090"
     CDS             1156..1887
                     /gene="bluR"
                     /locus_tag="OO515_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6574128.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2108..2512
                     /locus_tag="OO515_000091"
     CDS             2108..2512
                     /locus_tag="OO515_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6574129.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2565..2675
                     /locus_tag="OO515_000092"
     CDS             2565..2675
                     /locus_tag="OO515_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574130.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            2911..2955
                     /locus_tag="OO515_000093"
     CDS             2911..2955
                     /locus_tag="OO515_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574131.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3208..3531
                     /gene="iraM"
                     /locus_tag="OO515_000094"
     CDS             3208..3531
                     /gene="iraM"
                     /locus_tag="OO515_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6574132.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3634..4884)
                     /gene="icd"
                     /locus_tag="OO515_000095"
     CDS             complement(3634..4884)
                     /gene="icd"
                     /locus_tag="OO515_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6574133.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5056..5709
                     /gene="rluE"
                     /locus_tag="OO515_000096"
     CDS             5056..5709
                     /gene="rluE"
                     /locus_tag="OO515_000096"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6574134.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5719..6180
                     /gene="nudJ"
                     /locus_tag="OO515_000097"
     CDS             5719..6180
                     /gene="nudJ"
                     /locus_tag="OO515_000097"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6574135.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6234..7340
                     /gene="mnmA"
                     /locus_tag="OO515_000098"
     CDS             6234..7340
                     /gene="mnmA"
                     /locus_tag="OO515_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6574136.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7376..8017
                     /gene="hflD"
                     /locus_tag="OO515_000099"
     CDS             7376..8017
                     /gene="hflD"
                     /locus_tag="OO515_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6574137.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8021..9391
                     /gene="purB"
                     /locus_tag="OO515_000100"
     CDS             8021..9391
                     /gene="purB"
                     /locus_tag="OO515_000100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6574138.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9559..10230
                     /gene="phoP"
                     /locus_tag="OO515_000101"
     CDS             9559..10230
                     /gene="phoP"
                     /locus_tag="OO515_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6574139.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10230..11690
                     /gene="phoQ"
                     /locus_tag="OO515_000102"
     CDS             10230..11690
                     /gene="phoQ"
                     /locus_tag="OO515_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6574140.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            11766..12887
                     /gene="roxA"
                     /locus_tag="OO515_000103"
                     /gene_synonym="ycfD"
     CDS             11766..12887
                     /gene="roxA"
                     /locus_tag="OO515_000103"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6574141.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(12936..14162)
                     /gene="pepT"
                     /locus_tag="OO515_000104"
     CDS             complement(12936..14162)
                     /gene="pepT"
                     /locus_tag="OO515_000104"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6574142.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14412..15548
                     /gene="potA"
                     /locus_tag="OO515_000105"
     CDS             14412..15548
                     /gene="potA"
                     /locus_tag="OO515_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6574143.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15532..16389
                     /gene="potB"
                     /locus_tag="OO515_000106"
     CDS             15532..16389
                     /gene="potB"
                     /locus_tag="OO515_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6574144.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16386..17180
                     /gene="potC"
                     /locus_tag="OO515_000107"
     CDS             16386..17180
                     /gene="potC"
                     /locus_tag="OO515_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6574145.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17177..18223
                     /gene="potD"
                     /locus_tag="OO515_000108"
     CDS             17177..18223
                     /gene="potD"
                     /locus_tag="OO515_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6574146.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18379..19200)
                     /gene="cobB"
                     /locus_tag="OO515_000109"
     CDS             complement(18379..19200)
                     /gene="cobB"
                     /locus_tag="OO515_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6574147.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19216..20127)
                     /gene="nagK"
                     /locus_tag="OO515_000110"
     CDS             complement(19216..20127)
                     /gene="nagK"
                     /locus_tag="OO515_000110"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6574148.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20156..21400)
                     /gene="lolE"
                     /locus_tag="OO515_000111"
     CDS             complement(20156..21400)
                     /gene="lolE"
                     /locus_tag="OO515_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6574149.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21400..22101)
                     /gene="lolD"
                     /locus_tag="OO515_000112"
     CDS             complement(21400..22101)
                     /gene="lolD"
                     /locus_tag="OO515_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6574150.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22094..23293)
                     /gene="lolC"
                     /locus_tag="OO515_000113"
     CDS             complement(22094..23293)
                     /gene="lolC"
                     /locus_tag="OO515_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6574151.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23555..24628
                     /locus_tag="OO515_000114"
     CDS             23555..24628
                     /locus_tag="OO515_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6574152.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYLNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            24756..28202
                     /gene="mfd"
                     /locus_tag="OO515_000115"
     CDS             24756..28202
                     /gene="mfd"
                     /locus_tag="OO515_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6574153.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28346..29302
                     /gene="ldtC"
                     /locus_tag="OO515_000116"
                     /pseudo
     CDS             28346..29302
                     /gene="ldtC"
                     /locus_tag="OO515_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29385..29642)
                     /gene="bhsA"
                     /locus_tag="OO515_000117"
     CDS             complement(29385..29642)
                     /gene="bhsA"
                     /locus_tag="OO515_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6574154.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            29883..30515
                     /gene="comR"
                     /locus_tag="OO515_000118"
     CDS             29883..30515
                     /gene="comR"
                     /locus_tag="OO515_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6574155.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30577..31116)
                     /locus_tag="OO515_000119"
     CDS             complement(30577..31116)
                     /locus_tag="OO515_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6574156.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31343..32647)
                     /gene="ndh"
                     /locus_tag="OO515_000120"
     CDS             complement(31343..32647)
                     /gene="ndh"
                     /locus_tag="OO515_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6574157.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33055..33597)
                     /locus_tag="OO515_000121"
     CDS             complement(33055..33597)
                     /locus_tag="OO515_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6574158.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33620..34645)
                     /gene="nagZ"
                     /locus_tag="OO515_000122"
     CDS             complement(33620..34645)
                     /gene="nagZ"
                     /locus_tag="OO515_000122"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6574159.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34656..35480)
                     /gene="thiK"
                     /locus_tag="OO515_000123"
     CDS             complement(34656..35480)
                     /gene="thiK"
                     /locus_tag="OO515_000123"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6574160.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35461..36102)
                     /gene="lpoB"
                     /locus_tag="OO515_000124"
     CDS             complement(35461..36102)
                     /gene="lpoB"
                     /locus_tag="OO515_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6574161.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36116..36493)
                     /locus_tag="OO515_000125"
     CDS             complement(36116..36493)
                     /locus_tag="OO515_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6574162.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36496..36855)
                     /gene="hinT"
                     /locus_tag="OO515_000126"
     CDS             complement(36496..36855)
                     /gene="hinT"
                     /locus_tag="OO515_000126"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6574163.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37189..39378
                     /gene="fhuE"
                     /locus_tag="OO515_000127"
                     /pseudo
     CDS             37189..39378
                     /gene="fhuE"
                     /locus_tag="OO515_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39438..40871)
                     /gene="ptsG"
                     /locus_tag="OO515_000128"
     CDS             complement(39438..40871)
                     /gene="ptsG"
                     /locus_tag="OO515_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6574164.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41166..41963)
                     /locus_tag="OO515_000129"
     CDS             complement(41166..41963)
                     /locus_tag="OO515_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6574165.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(41974..42978)
                     /gene="holB"
                     /locus_tag="OO515_000130"
     CDS             complement(41974..42978)
                     /gene="holB"
                     /locus_tag="OO515_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6574166.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(42975..43616)
                     /locus_tag="OO515_000131"
     CDS             complement(42975..43616)
                     /locus_tag="OO515_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6574167.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43606..44628)
                     /gene="yceG"
                     /locus_tag="OO515_000132"
     CDS             complement(43606..44628)
                     /gene="yceG"
                     /locus_tag="OO515_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6574168.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44631..45440)
                     /gene="pabC"
                     /locus_tag="OO515_000133"
     CDS             complement(44631..45440)
                     /gene="pabC"
                     /locus_tag="OO515_000133"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6574169.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45560..46801)
                     /gene="fabF"
                     /locus_tag="OO515_000134"
     CDS             complement(45560..46801)
                     /gene="fabF"
                     /locus_tag="OO515_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6574170.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(46889..47125)
                     /gene="acpP"
                     /locus_tag="OO515_000135"
     CDS             complement(46889..47125)
                     /gene="acpP"
                     /locus_tag="OO515_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6574171.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47336..48070)
                     /gene="fabG"
                     /locus_tag="OO515_000136"
     CDS             complement(47336..48070)
                     /gene="fabG"
                     /locus_tag="OO515_000136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6574172.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48083..49012)
                     /gene="fabD"
                     /locus_tag="OO515_000137"
     CDS             complement(48083..49012)
                     /gene="fabD"
                     /locus_tag="OO515_000137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6574173.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49028..49981)
                     /gene="fabH"
                     /locus_tag="OO515_000138"
     CDS             complement(49028..49981)
                     /gene="fabH"
                     /locus_tag="OO515_000138"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6574174.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50049..51119)
                     /gene="plsX"
                     /locus_tag="OO515_000139"
     CDS             complement(50049..51119)
                     /gene="plsX"
                     /locus_tag="OO515_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6574175.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51200..51373)
                     /gene="rpmF"
                     /locus_tag="OO515_000140"
     CDS             complement(51200..51373)
                     /gene="rpmF"
                     /locus_tag="OO515_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6574176.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51425..51946)
                     /gene="yceD"
                     /locus_tag="OO515_000141"
     CDS             complement(51425..51946)
                     /gene="yceD"
                     /locus_tag="OO515_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6574177.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52145..52729
                     /gene="yceF"
                     /locus_tag="OO515_000142"
     CDS             52145..52729
                     /gene="yceF"
                     /locus_tag="OO515_000142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6574178.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(52841..53800)
                     /gene="rluC"
                     /locus_tag="OO515_000143"
     CDS             complement(52841..53800)
                     /gene="rluC"
                     /locus_tag="OO515_000143"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6574179.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54373..57558
                     /gene="rne"
                     /locus_tag="OO515_000144"
     CDS             54373..57558
                     /gene="rne"
                     /locus_tag="OO515_000144"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6574180.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57753..58706)
                     /gene="flgL"
                     /locus_tag="OO515_000145"
     CDS             complement(57753..58706)
                     /gene="flgL"
                     /locus_tag="OO515_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6574181.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58718..60361)
                     /gene="flgK"
                     /locus_tag="OO515_000146"
     CDS             complement(58718..60361)
                     /gene="flgK"
                     /locus_tag="OO515_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6574182.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60427..61368)
                     /gene="flgJ"
                     /locus_tag="OO515_000147"
     CDS             complement(60427..61368)
                     /gene="flgJ"
                     /locus_tag="OO515_000147"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6574183.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61368..62465)
                     /gene="flgI"
                     /locus_tag="OO515_000148"
     CDS             complement(61368..62465)
                     /gene="flgI"
                     /locus_tag="OO515_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6574184.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62477..63175)
                     /gene="flgH"
                     /locus_tag="OO515_000149"
     CDS             complement(62477..63175)
                     /gene="flgH"
                     /locus_tag="OO515_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6574185.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LIVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63228..64000)
                     /gene="flgG"
                     /locus_tag="OO515_000150"
                     /pseudo
     CDS             complement(63228..64000)
                     /gene="flgG"
                     /locus_tag="OO515_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64172..64927)
                     /gene="flgF"
                     /locus_tag="OO515_000151"
     CDS             complement(64172..64927)
                     /gene="flgF"
                     /locus_tag="OO515_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6574186.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(64947..66152)
                     /gene="flgE"
                     /locus_tag="OO515_000152"
     CDS             complement(64947..66152)
                     /gene="flgE"
                     /locus_tag="OO515_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6574187.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66177..66872)
                     /gene="flgD"
                     /locus_tag="OO515_000153"
     CDS             complement(66177..66872)
                     /gene="flgD"
                     /locus_tag="OO515_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6574188.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(66884..67288)
                     /gene="flgC"
                     /locus_tag="OO515_000154"
     CDS             complement(66884..67288)
                     /gene="flgC"
                     /locus_tag="OO515_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6574189.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67292..67708)
                     /gene="flgB"
                     /locus_tag="OO515_000155"
     CDS             complement(67292..67708)
                     /gene="flgB"
                     /locus_tag="OO515_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6574190.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            67863..68522
                     /gene="flgA"
                     /locus_tag="OO515_000156"
     CDS             67863..68522
                     /gene="flgA"
                     /locus_tag="OO515_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6574191.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVQDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68598..68891
                     /gene="flgM"
                     /locus_tag="OO515_000157"
     CDS             68598..68891
                     /gene="flgM"
                     /locus_tag="OO515_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6574192.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            68896..69312
                     /gene="flgN"
                     /locus_tag="OO515_000158"
     CDS             68896..69312
                     /gene="flgN"
                     /locus_tag="OO515_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6574193.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69352..70887)
                     /gene="murJ"
                     /locus_tag="OO515_000159"
     CDS             complement(69352..70887)
                     /gene="murJ"
                     /locus_tag="OO515_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6574194.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(70997..71920)
                     /locus_tag="OO515_000160"
     CDS             complement(70997..71920)
                     /locus_tag="OO515_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6574195.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(71922..72569)
                     /locus_tag="OO515_000161"
     CDS             complement(71922..72569)
                     /locus_tag="OO515_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6574196.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72580..73164)
                     /gene="rimJ"
                     /locus_tag="OO515_000162"
     CDS             complement(72580..73164)
                     /gene="rimJ"
                     /locus_tag="OO515_000162"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6574197.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73400..74608
                     /gene="mdtH"
                     /locus_tag="OO515_000163"
     CDS             73400..74608
                     /gene="mdtH"
                     /locus_tag="OO515_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6574198.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74672..75319
                     /gene="grxB"
                     /locus_tag="OO515_000164"
     CDS             74672..75319
                     /gene="grxB"
                     /locus_tag="OO515_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6574199.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75453..76013
                     /locus_tag="OO515_000165"
     CDS             75453..76013
                     /locus_tag="OO515_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6574200.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76119..77165
                     /gene="pyrC"
                     /locus_tag="OO515_000166"
     CDS             76119..77165
                     /gene="pyrC"
                     /locus_tag="OO515_000166"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6574201.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77239..77484
                     /gene="dinI"
                     /locus_tag="OO515_000167"
     CDS             77239..77484
                     /gene="dinI"
                     /locus_tag="OO515_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6574202.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            77774..78028
                     /gene="bssS"
                     /locus_tag="OO515_000168"
     CDS             77774..78028
                     /gene="bssS"
                     /locus_tag="OO515_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6574203.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78143..79261
                     /gene="solA"
                     /locus_tag="OO515_000169"
     CDS             78143..79261
                     /gene="solA"
                     /locus_tag="OO515_000169"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6574204.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19527 a        20860 c        19655 g        19291 t
ORIGIN      
        1 gcctgctcgc cgttttggca aagcgggaat ggaattattt gatttgcgcc tgcacgagcg
       61 agatgacgtt ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga
      121 tgacagagcg ctacaatttt ttcgtacttt tgtccttgca accgaacaat caacctattt
      181 cgagatccct gccgaagact cctggctttt tatcgctgac ggatctgata aagaacttga
      241 ttcctgtacc ctttcaccaa ctataaacga ccactttgcc tttcatccct tatcgcggcg
      301 gataatcgct tttgaagcca ttgtgcaaaa aaatgaagcc catcagccat agcggttggg
      361 cagcgtaaag acggggaaat ctacacagcg gatctcaaaa gtaaggcgct tgcattcgcg
      421 atggcacacg cacttgagct cggtgataaa atgatttcaa tcaatctatt acctatgacc
      481 ctggttaacg aacctgacgc agtctctttt ttacttgatg aaataaaggc caatgctctg
      541 gtgcctgaac aaatcatcgt tgaatttact gaaagtgaag ttatatctcg gtttgatgag
      601 tttgcagaag cgattaaatc gcttaaggct gtcggtatca gtgtagcaat tgatcatttt
      661 ggcgcaggtt ttgccggttt gttactcctg tcacgcttcc agcctgacag aattaaaatc
      721 agtcaggaat tgattaccaa tgttcataaa agcgggccac ggcaggcaat tattcaagcg
      781 atcataaaat gctgtacatc acttgaaatt caagtcagtg ctatgggcgt ggcaacacca
      841 gaagagtgga tgtggcttga atccgcagga attgagatgt ttcagggaga tctgtttgct
      901 aaagctaaat tgaatggtat cccttccata gcgtggccag agaaaaaata attttcagca
      961 cattctttca catgatttca gtaaatcatc gtgaaaatga atacgttatg tttaaattta
     1021 atccacatta aatataatgt ttcatccaat ttagctgaaa tcttatttat actttgtaca
     1081 attttagcgt atattgtaca aagtattatt gggtcgtgta caggcggcgg agatttatga
     1141 ccgcaaggag gaattgtggc ttattacagc attggtgatg ttgctgaacg ttgcgggatt
     1201 aatcctgtca ctctccgggc ctggcaacgc cgctacggtt tgttaaaacc acagcgcagt
     1261 gaaggcggac accgactctt tgatgaagaa gacatacaac gcatcgaaga gatcaagcgt
     1321 tggataagta atggcgtccc tgtaggcaaa gttaaagcat tactggaaac caccagccag
     1381 gatacggaag atgactggaa ccgcctgcaa gaagagatga tgtcaattct tcgtatggct
     1441 aatcctgcca aactacgcgc gagaattatt tcactgggtc gagagtaccc agtagatcaa
     1501 ttgattaatc atgtttacct tcctgttcgc cagcgtctcg tgcttgatca caacacctcc
     1561 cgcattatga gcagtatgtt tgacggcgca ttaattgaat atgcagcagc ctcacttttc
     1621 gaaatgcgcc gtaagcccgg taaagaagcc attctgatgg cgtggaatgt tgaagagaga
     1681 gcacgattgt ggctggaagc atggcgttta tcattgtcag gatggcacat ttctgttctt
     1741 gctgatccca ttgaatcgcc gcacccggaa ctgttcccga cgcaaacatt gattgtctgg
     1801 acaggcatgg caccaacgag aaggcaaaac gaacttttac aacattgggg tgagcaaggt
     1861 tataaagtca tctttcatgc cccctaattt cacctttatc ccgcccctta gacagaatgt
     1921 tataatctga acccacaaca gcatttgtac cggagctggt ccgttgcaaa atgctcaccg
     1981 gaagaattca ggtaatccct ctatcacact ctgtacatat cacagccaaa ggacagacca
     2041 gattgaagtg gtaaaaccga tcgtgcacac tacgcagtct cacaactaac aagaggttat
     2101 tatcctgatg gatcaggtcg ttgtttttca aaagatgttt gagcaagtga gaaaagagca
     2161 aaacttctcc tggttttatt cagaattaaa acatcaccgt attgcacatt acatttatta
     2221 tctggctacg gataacatca gaattattac tcacgatgac acggttttgt tattaagagg
     2281 aaccaggaac ctgttaaaag ttagtacgac caagaaccct gctaaaataa aagaggccgc
     2341 attgcttcat atttacggaa aatctacatt tcgggaatac tgttcaacac tggcaggcgc
     2401 gggcgttttc cgttgggtta ctgatgttaa tcataacaaa cgcagttact acgccattga
     2461 taatacgctt ttatatattg aagatgtaga aaataataaa ccattaatct agctaaagtt
     2521 ggatgcttaa gaaatgcttc ataattcagt aaggcattag cataatggga ataaaagtac
     2581 agagactatc cctatggatg ataaatactg tccttttatt gctacccata aataatcacc
     2641 aggccaatac tatcaaattg atatttgaaa tgtgatcact tgactttcta tacgttattt
     2701 tataacggtt aatatattta taaaaacaac gggtgtgcca tacgcccgtt tcaatactta
     2761 acgcacatgt gttttggttt agtcatcatc cagttatatg tattttagcc aggaacaggt
     2821 taaacctttc ctatataact caaaaattga aaccttattc tcatgtcatg tttatattca
     2881 ttattatcgt tatataaaaa ggcaaccata atgtttagca aattggcacc aagtagcata
     2941 aaggctatgt tttaattaca ggatgttcag tcatttgaat gtataacatt atagctaaac
     3001 aaatctaaaa cgaagtgaat aatttattgt tttcactaaa tctcattttg tttaacatcc
     3061 attgagattc cttgctttaa attttgtttt atataagcaa tcgttttaat taatttattt
     3121 tttgaagggg taatatactc atatgcaaaa taaagaaatg aacatccaaa tgaactatat
     3181 taaataccgt gggataagac ataacaaatg aagtgggtag taattgacac ggtaattcaa
     3241 cctacatgcg gcatatcttt ttcagccata tggggtgata tgaaaaagat catctggtat
     3301 caatctacta tatttcttcc tcctggaagt atatttacac cggttaagct tggtattatc
     3361 cttaaggaaa aagaatatcc tattactatt tataacattg caccattcaa caagaattta
     3421 tggagtttac tcaaaagcag tcaagagtgt cctccaggag aaagcaaaat aacaaataaa
     3481 tgtttacata atagttgcat tataaaaata tgcccatatg ggctcaagta atgggcttca
     3541 tataaatatg ctcgatatgc tattaataat atccacataa ataaaacaac gggcgtgtta
     3601 tacgcccgtt tcaatattta actcatggag agattacatg ttttcgatga tcgcttcacc
     3661 aaactctgaa catttcagca gcttagcgcc ttccatcaga cgttcaaagt cataagttac
     3721 ggttttcgcg ttgatagcac cttccatacc tttaacaatt aaatcagccg cttcagtcca
     3781 ccccatatgg cgcagcatca tctcagcgga gaggatgata gagcctggat ttactttgtc
     3841 ctgaccggca tatttcggcg cagtaccgtg ggtggcttca aacagggcgc attcgtcacc
     3901 gatgtttgcg ccaggggcga taccgatacc gccaacctgc gctgccaggg cgtcagaaat
     3961 gtagtcaccg ttcaggttca tacaggcgat aacatcatat tcagccggac gcagcaggat
     4021 ctgttgcagg aatgcatcag caatcacgtc tttaatgacg atctctttgc cggtgttcgg
     4081 gtttttaact ttcagccacg ggccgccgtc gatcagttca ccgccaaact cttcacgcgc
     4141 cagctggtag ccccagtctt taaacgcgcc ttcggtgaac ttcatgatgt tgcctttgtg
     4201 caccagagtc acagagtcac gatcgttagc aattgcgtat tcgatcgctg cacgaaccag
     4261 acgtttggtg ccttcttccg aacacggctt aataccgata ccacaatgtt ccgggaagcg
     4321 aattttcttc acccccatct cttcacgcag gaatttaatc actttctcgg cgtcggcaga
     4381 gtctgctttc cattcgatac ccgcataaat gtcttccgag ttttcacgga agataaccat
     4441 atcggtcagt tcagggtgtt taaccgggct tggagtgccc tgatagtaac gtaccggacg
     4501 caggcagatg tagagatcca gttcctggcg cagggcaacg ttcagagagc gaataccgcc
     4561 accaacagga gtggtcagcg gacctttaat ggcaacgcga tattcacgaa tcagatcaag
     4621 ggtttcagca ggcagccaga catcctgacc ataaacctgt gtggattttt caccggtgta
     4681 aatttccatc caggagattt tacgctcgcc tttataggct ttctcgactg cagcgtcgac
     4741 cactttcagc atggctgggg ttacatctac accgattcca tcaccttcaa tgtaagggat
     4801 aatcggattt tcaggaacgt tgagtttgcc gttttgcagg gtgatcttct tgccttgtgc
     4861 cggaacaact actttacttt ccattcacct ctccttcgag cgctactggt ttgctcgtct
     4921 gccaccacgt tgcatatgcg tttgcgtcct gcgatacgga tgctttagag caattttttg
     4981 ttaatgattt gtaattggct tgtcaatgct accctattgt ttgccgtcat gaaagatgcg
     5041 cgttattagg ctataatgcg gcaattcata atctctgaaa ataccatgca aaaaacttct
     5101 tttagaaatc accaggttaa gcgattcagc tcgcaacgtt ctactaggcg taaacctgaa
     5161 aaccagccca cgcgtgtgat cctgttcaat aaaccctacg atgttcttcc gcagttcacc
     5221 gatgaagccg gacgcaaaac attaaaagaa ttcatcccgg ttcagggcgt ttatgcagca
     5281 ggtcgtcttg accgcgatac cgaagggtta ctggtgctga ccaataacgg cgcgttgcag
     5341 gcgcgtttaa cccagccggg caaacgcacc gggaaaatct attatgtgca ggtggaaggt
     5401 attcccacac aagacgcact tgaagccttg cgcaatggcg taaccttaaa tgatggccct
     5461 accctgcccg ccggcgcgga gctggttgac gaaccagcgt ggttatggcc gcggaatcca
     5521 ccaattcgtg aacgcaaaag tattcccacc agctggctga agatcacctt atatgaagga
     5581 cgtaatcgcc aggtgcgccg catgaccgcc catgttggtt tccccacgct gcgactgatt
     5641 cgttatgcga tgggtgatta cactttggat aaccttgcca atggcgaatg gcgagaagca
     5701 acagaataag gatgcacaat gtttaaaccg cacgttaccg ttgcttgcgt agtgcacgca
     5761 gagggtaaat ttttagtcgt tgaagagacg attaatggta aagcgttatg gaaccaacct
     5821 gccgggcatc tggaagccga tgaaacctta gtggaagctg ccgcccgtga gctatgggaa
     5881 gaaaccggca tcagcgcgca accgcaacac tttattcgta tgcatcagtg gattgcgcca
     5941 gataaaacgc cgtttttgcg tttcctcttt gccattgagc ttgagcaaat atgcccgacc
     6001 cagcctcatg acagcgatat cgactgctgt cgttgggtca gcgccgaaga aattttaaag
     6061 gcgtcaaatc ttcgttcgcc gctggtggcg gaaagtatcc gttgttatca aagtgggcaa
     6121 cgctatccgc tggagatgat tggcgatttt aactggcctt ttacaaaggg tgtcatctaa
     6181 aaggctgcgt gatagaatac gccgccttga agttcaatgt cgtgagtgat ccaatgtctg
     6241 aaaccgcaaa aaaagtaatc gtcggcatgt ccggcggtgt cgattcctcc gtttctgcct
     6301 ggctgttgca acaacaggga tatcaggttg aaggcctgtt tatgaagaac tgggaagaag
     6361 acgacggtga ggaatattgc acagcggcag cggatctggc tgatgcccag gctgtctgcg
     6421 acaagctcgg cattgaactg cacaccgtta actttgctgc cgagtactgg gacaatgtct
     6481 tcgaactgtt ccttgccgaa tataaagccg gtcgcacgcc gaatccggat attctgtgca
     6541 acaaagagat caaatttaaa gctttcctcg aatttgccgc cgaagattta ggtgccgatt
     6601 atatcgctac cggtcattac gtacgtcggg ccgatgtcga tggcaagagc cgcctgctgc
     6661 gtggtctgga cagcaataaa gaccagagct acttccttta tacgctcagc catgagcaga
     6721 ttgcgcaaag cctgttcccg gtcggcgaac tggaaaaacc gcaggtgcgt aagattgctg
     6781 aagatcttgg tctggtcacc gcgaagaaaa aagactctac cggcatttgc ttcattggcg
     6841 aacgtaaatt ccgcgagttc ctgggccgtt atctcccggg gcaaccgggc aaaatcatta
     6901 ccgtcgatgg cgatgaaatt ggtgagcacc aggggctgat gtatcacact ctcggtcagc
     6961 gtaaaggtct gggtatcggt ggcaccaaag aaggcaccga agaaccttgg tatgtggtgg
     7021 acaaagacgt cgaaaacaac attctggttg tcgctcaggg ccatgaacac ccgcggctga
     7081 tgtctgtcgg gttgattgcc cagcagttgc actgggtcga tcgcgaacca ttcaccggca
     7141 ctatgcgttg cacggtaaaa acccgctatc gccagaccga tatcccttgc accgtcaagg
     7201 cgctggacga tgatcgcatt gaagtgattt tcgatgaacc ggttgccgcc gtgacgccgg
     7261 gccagtctgc cgtcttctat aacggtgaag tgtgcctcgg tggcggtatt attgagcagc
     7321 gtctgccgct gccggtctga ttattatctt tacttaaaac aaggaagcag tgaacgtggc
     7381 aaagaattac tatgacatca ccctcgccct ggccggtatt tgtcagtcgg cacgcctggt
     7441 gcaacaactc gctcaccagg ggcattgtga tgccgatgcg ctacacgtct cactcaacag
     7501 tattattgat atgaacccca gctcgacgct ggcggttttt ggcggtagcg aagccaacct
     7561 gcgcgtcggg ctggaaaccc tgctcggcgt gctcaatgcc agcagtcgcc agggcttaaa
     7621 cgccgaatta acccgctaca cactcagctt gatggtgctt gagcgcaaac tctcctcagc
     7681 gaaaggcgcg ctcgacactc tgggcaaccg gatcaacggc ctgcaacgcc agctcgaaca
     7741 cttcgattta cagtccgaaa cgctgatgag cgcgatggct gctatctatg ttgatgtgat
     7801 cagcccgctt ggcccacgca ttcaggtcac cggttcccct gctgtactgc aaagcccaca
     7861 agtgcaggcg aaagttcgcg caaccctgct ggcaggcatt cgcgccgccg tgctctggca
     7921 ccaggtcggc ggcggacgtc tgcaactgat gttttctcgt aatcgcctga ccactcaggc
     7981 aaaacaaatt cttgctcatt taaccccgga gttgtgatct atggaattat cctcactgac
     8041 cgccgtttcc cctgtcgatg gacgctacgg cgataaagtc agcgcgctgc gcgggatttt
     8101 cagcgaatat ggtttgctga aattccgtgt acaagttgaa gtacgttggc tgcaaaaact
     8161 ggccgcgcac gcagcgatca aggaagttcc tgcttttgct gccgacgcaa tcggttacct
     8221 tgatgcaatt gtcgccaatt tcagcgaaga agatgccgca cgcatcaaaa ccatcgagcg
     8281 taccactaac cacgacgtta aagcggttga gtatttcctg aaagaaaaag tggcggagat
     8341 cccggaactg cacgcggttt ctgaattcat ccactttgcc tgtacttcgg aagatatcaa
     8401 taacctctcc cacgcattaa tgctgaaaac cgcgcgtgat gaagtgatcc tgccgtactg
     8461 gcgtcaactg attgatggca ttaaagatct cgccgttcag taccgcgata tcccgctgct
     8521 gtctcgtacc cacggtcagc cagccacgcc gtcaaccatc ggtaaagaga tggcaaacgt
     8581 cgcctaccgt atggagcgcc agtaccgcca gcttaaccag gtggagatcc tcggcaaaat
     8641 caacggcgcg gtcggtaact ataacgccca catcgccgct tacccggaag ttgactggca
     8701 tcagttcagc gaagagttcg tcacctcgct gggtattcaa tggaacccgt acactaccca
     8761 gattgaaccg cacgactaca tcgccgagct gtttgattgc gttgcacgct tcaacaccat
     8821 tctgatcgac tttgatcgtg acgtctgggg ttatatcgcc cttaaccact tcaaacagaa
     8881 aaccattgct ggtgagattg gttcttccac catgccgcat aaagttaacc cgatcgactt
     8941 cgagaactcc gaagggaatc tgggcctttc caacgcggta ttacagcatc tggcaagcaa
     9001 actgccggtt tcccgctggc agcgtgacct gaccgactcc accgtgctgc gtaacctcgg
     9061 cgtgggtatc ggttatgcgc tgattgcgta tcaatccacc ctgaaaggcg tgagcaaact
     9121 ggaagtgaac cgtgaccatc tgctggatga gctggatcac aactgggaag tgctggctga
     9181 gccaatccag acagttatgc gtcgctatgg catcgaaaaa ccgtacgaga agctgaaaga
     9241 gctgactcgt ggtaagcgcg ttgacgccga aggcatgaag cagtttatcg acggtctggc
     9301 gttgccagaa gaagagaaag cccgcctgaa agcgatgacg ccggcaaact acattggtcg
     9361 cgccatcacc atggttgatg agctgaaata aactcgtatc agtgccggat ggcgatgctg
     9421 tccggcctgc ttattaagat tatccgcttt ttattttttc actttacctc ccctccccgc
     9481 tggtttattt aatgtttacc cccataacca cataatcgcg ttacactatt ttaataatta
     9541 agacagggag aaataaaaat gcgcgtactg gttgttgaag acaatgcgtt gttacgtcac
     9601 caccttaaag ttcagattca ggatgctggt catcaggtcg atgatgcaga agatgccaaa
     9661 gaagccgatt attatctcaa tgaacattta ccggatattg cgattgtcga tctcggattg
     9721 ccagacgagg acggtctgtc actgattcgc cgctggcgta gcaatgatgt ttcactgccg
     9781 attctggtat taaccgcccg tgaaagctgg caggacaaag tcgaagtatt aagtgctggt
     9841 gctgatgatt acgtgactaa accatttcat attgaagagg tgatggcgcg aatgcaggca
     9901 ttaatgcggc gtaatagcgg tctggcttca caggtcattt cgctaccacc gtttcaggtt
     9961 gatctctctc gccgtgaatt atctattaat gacgaagtga tcaaactgac cgcgttcgaa
    10021 tacaccatta tggaaacgtt gatacgcaat aatggcaaag tggtgagcaa agattcgtta
    10081 atgctccaac tctatccgga tgcggagctg cgggaaagcc ataccattga tgtactgatg
    10141 ggacgtctgc gcaaaaaaat tcaggcacaa tatccgcaag aagtgattac taccgttcgc
    10201 ggccagggct atttgttcga attgcgctga tgaaaaaatt actgcgtctt tttttcccgc
    10261 tctcgctgcg ggtacgtttt ctgttggcaa cggctgcggt agtactggtg ctttcgcttg
    10321 cctacggaat ggtcgcgctg atcggttata gcgtcagttt cgataaaact acgtttcggc
    10381 tgttacgtgg cgagagcaat ctgttctata cccttgcgaa gtgggaaaac aataagttgc
    10441 atgtcgagtt acccgaaaat atcgacaagc aaagccccac catgacgcta atttatgatg
    10501 agaacgggca gcttttatgg gcgcaacgtg acgtgccctg gctgatgaag atgatccagc
    10561 ctgactggct gaaatcgaat ggttttcatg aaattgaagc ggatgttaac gataccagcc
    10621 tcttgctgag tggagatcat tcgatacagc aacagttgca ggaagtgcgg gaagatgatg
    10681 acgacgcgga gatgacccac tcggtggcag taaacgtcta cccggcaaca tcgcggatgc
    10741 caaaattaac cattgtggtg gtggatacca ttccggtgga gctaaaaagt tcctatatgg
    10801 tctggagctg gtttatctat gtgctctcag ccaatctgct gttagtgatc ccgctgctgt
    10861 gggtcgccgc ctggtggagt ttacgcccca tcgaagccct ggcaaaagaa gtccgcgaac
    10921 tggaagaaca taaccgcgaa ttgctcaatc cagccacaac gcgagaactg accagtctgg
    10981 tacgaaacct gaaccgattg ttaaaaagtg aacgcgaacg ttacgacaaa taccgtacga
    11041 cgctcaccga cctgacccat agtctgaaaa cgccactggc ggtgctgcaa agtacgctgc
    11101 gttctctgcg tagtgaaaag atgagcgtca gtgatgctga gccggtaatg ctggagcaaa
    11161 tcagccgcat ttcacagcaa attggctact acctgcatcg tgccagtatg cgcggcggga
    11221 cattgctcag ccgcgagctg catccggtcg ccccactgct ggacaatctc acctcagcgc
    11281 tgaacaaagt gtatcaacgc aaaggggtca atatctctct cgatatttcg ccagagatca
    11341 gctttgtcgg tgagcagaac gattttgtcg aggtgatggg caacgtgctg gataatgcct
    11401 gtaaatattg cctcgagttt gtcgaaattt ctgcaaggca aaccgacgag catctctata
    11461 ttgtggtcga ggatgatggc cccggtattc cattaagcaa gcgagaggtc attttcgacc
    11521 gtggtcaacg ggttgatact ttacgccctg ggcaaggtgt agggctggcg gtagcccgcg
    11581 aaatcaccga gcaatatgag ggtaaaatcg tcgccggaga gagcatgctg ggcggtgcgc
    11641 ggatggaggt gatttttggt cgccagcatt ctgcgccgaa agatgaataa atatgtccat
    11701 acttcacgca ttacgttaag catccgttat aatcggttgc agataccagc ctgtggatgc
    11761 ttaacatgga ataccaactc actcttaact ggcccgattt tcttgaacgt cactggcaga
    11821 aacgcccggt ggtgttaaaa cgcggcttta ataattttat tgacccgatc tctccagacg
    11881 agttggcggg tctggcgatg gaaagcgaag ttgacagtcg actggtcagt caccaggatg
    11941 gcaaatggca ggtcagccac ggcccgttcg aaagctacga tcatctcggt gaaaccaact
    12001 ggtcattact ggtacaggca gtgaaccact ggcatgagcc gaccgccgcg ctgatgcgac
    12061 cgttccgtga actaccggac tggcgtattg atgatctgat gatttctttt tctgtacctg
    12121 gcggcggcgt cggcccgcat ctcgatcagt acgacgtgtt tatcattcag ggtaccggac
    12181 gtcgtcgctg gcgagtgggc gaaaagctgc aaatgaaaca gcactgccca catccggatc
    12241 tgttacaggt cgatccgttc gaagccatca tcgatgaaga gctggagcct ggtgatattc
    12301 tttatattcc gccaggattc ccgcatgaag gctacgcgct ggaaaatgcg atgaactatt
    12361 ccgtgggctt tcgcgcgcca aatacgcggg aactgattag tggatttgcc gattatgtgc
    12421 tgcaacgtga actgggcggc aactactaca gcgatcctga tgttccacct cgcgctcatc
    12481 ctgcggatgt tctgcctcaa gagatggata aactgcgtga gatgatgctc gaattgatca
    12541 accagccgga acactttaag caatggtttg gcgagtttat atcccagtca cgtcatgaac
    12601 tggatatcgc gccgccagaa ccgccttatc agccagatga aatctacgat gcgctgaaac
    12661 aaggtgatgt gctggtgcgc ctgggtggtc tgcgcgtatt gcgcattggc gacgacgtgt
    12721 atgccaatgg tgagaagatc gattccccgc accgtccggc actggatgca ctcgccagca
    12781 acattgcgct gactgcggag aattttggcg atgcgctgga agatccgtca ttcctcgcga
    12841 tgctcgcggc gctggtcaat agcgggtatt ggttcttcga agggtaagtt tggatttaag
    12901 ccggatgcgg catcacatgc cgcatccctt ttcgcttact tccgttgcgc cgttaactcg
    12961 gcaatacgga cgatcacctg caccgctttt tccatacctt ccagagtcac aaactcatgc
    13021 ttaccatgat agttgtaacc gccagtgaac aagttcgggc acggtaatcc cataaacgac
    13081 aactgcgcgc cgtcggtacc accgcggatc ggtttcagtt ccggttcaat atcgcagtca
    13141 cgcatcgcct gctgggcgat atcgagaata tgcggatgct caaccacttt ctctcgcata
    13201 ttgtagtaac tgtcttcaat caccagttca atgtagcaat caggatgtaa ccctttgccc
    13261 acttttttgg cgatctccat cattttacgt ttacgcgctt caaactgttt acggtcgaaa
    13321 tcacggatga tgtagtgcat atcggcccgt tcaacggtgc ctttcatgct cgccaggtga
    13381 tagaaacctt catagccttc tgtcatttcc gggctttcat ccgccggaac ttccgcatga
    13441 atacgtgccg ccagcgacag cgcatttacc atcactcctt tcgccgtgcc cggatgaaca
    13501 ttgttaccga caattttgat attgaccgat gcggcgttga agttttcaaa ctccagttcg
    13561 cctacgccac caccgtcaac agtgtaagcc caacgggcat cgaaggcatc aacatcaaaa
    13621 tgtttcgccc ctttgcccac ttcttcatcc ggggtaaagg cgacgcgaat atcaccatgc
    13681 ggaatgtttt tctgttgcaa taccgccagc gcggtcatga tttctgcaat acctgctttg
    13741 tcatcggcac ctaacaaggt tttaccatcg gtggtaatca acgtctgacc cagtagctga
    13801 tgcagcaccg ggaacataac cggtgataaa acttcatcgc cgatacccag cgcaatatcg
    13861 ccaccgcgat agttttcaac aatttgcgga ttaacatttt tgccgctgca atccggtgag
    13921 gtatccacat gagaaataaa gccaatcgcc gggatatcgc cagggacgtt agccggtaac
    13981 gtcgccatca aagtgccctt ctcacttaag gtcacattga taagccccat ttcttcgagc
    14041 tgctctttca gcagatgcaa taacttccat tggccttccg tgctgggaac ctgtctcacc
    14101 cctgcttttg attgggtatc cagagatacg tagttcaaaa atcgctcaag tagtttatcc
    14161 atgtagtcac cctcactttt tgtgacaaca ttattaagaa gcaagaaaag acaaatattg
    14221 cgtcaggtca cttttacccc tgcaagcggg aatatttatc agcattacct taatgaatat
    14281 aaagctaagc cagtaattca caacaaagat tggcgattca agcggtttgc cgtagaatta
    14341 ccgccctcat taagagtcac caaggtggtt aaccacaaac cccgcatcgg taagccatcc
    14401 gttgcgttta catgggacag agtaaaaaat tgaataaaca accgagttcg ctttcaccgc
    14461 tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa agaggtcatt ccccagctgg
    14521 atctgactat caacaatggc gagttcctca cgctgcttgg cccttctggc tgcggtaaaa
    14581 caaccgttct tcgcctgatt gcaggtctgg aaactgttga ttccggacgc atcatgctgg
    14641 ataacgagga catcacccac gttccggcgg aaaaccgcta tgtgaacact gttttccaaa
    14701 gctacgcact tttcccccac atgaccgtgt tcgaaaatgt ggcctttggg ttgcgcatgc
    14761 aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga agccctgcgg atggtgcaac
    14821 tggaaacctt cgctcaacgt aaaccgcatc agctctctgg tggtcaacag caacgcgtcg
    14881 ccattgctcg cgcggtggtt aacaagcctc gtctgttgtt gctggatgag tcgctctcag
    14941 cgctggatta caaactgcgt aagcaaatgc agaacgagct gaaagcgtta cagcgtaagc
    15001 ttggtattac cttcgtcttt gtcactcacg atcaggaaga agcgctcacc atgtcagaca
    15061 ggattgtggt gatgcgcgag ggtcgtattg agcaagatgg tacaccgcgc gaaatttacg
    15121 aagagccgaa aaacctgttt gttgccggct tcattggcga aatcaatatg tttaacgcca
    15181 ctgtcatcga acgtctcgac gagcagcgcg tacgcgccaa cgttgaaggc cgcgaatgta
    15241 atatctacgt taacttcgcc gttgaaccgg ggcaaaaact gcatgttctg ctgcgcccgg
    15301 aagacttacg tgttgaagag attaacgacg acaaccacgc tgaagggctg attggttacg
    15361 ttcgtgagcg taactacaaa ggcatgacgc tggagtcggt tgttgaactg gaaaatggca
    15421 agatggtgat ggtcagcgaa ttcttcaatg aagacgaccc tgactttgac cactctctcg
    15481 accaaaaaat ggccattaat tgggtagaaa gctgggaggt cgtactggct gatgaagaac
    15541 acaagtaagt tccagaatgt agtgattgtc actattgtcg gttggcttgt gttgtttgtc
    15601 tttctgccca acctgatgat cattggcacc agctttttga cccgcgacga cgccagtttc
    15661 gtcaaaatgg tctttacgct ggataactac acgcgtctgc tcgatccgct ctattttgaa
    15721 gtgctattgc actcgctgaa tatggcgctg atcgccaccc tcgcctgcct ggtgctgggc
    15781 tacccgtttg cctggtttct ggcgaagctg ccacacaagg tgcgtccgct gctgctgttt
    15841 ctgctgattg ttcccttctg gaccaactca ctgattcgca tctacgggct gaaaattttc
    15901 ctcagcacca aaggctatct caacgagttt ttgctctggc tgggcgttat cgacacacca
    15961 attcgtatca tgttcacgcc cagtgcggtg attatcggtc tggtttacat tctgctaccg
    16021 tttatggtga tgccgctgta ctccagtatc gaaaaactgg ataagccgtt gcttgaggcg
    16081 gcgcgcgatc tcggtgccag caagttacag acttttatcc gtatcattat tccactgacg
    16141 atgccgggaa ttattgccgg atgtctgctg gtgatgctgc cagcgatggg cctgttctat
    16201 gtatccgacc tgatgggcgg tgcgaaaaac ctgctgatcg gtaacgtcat caaggtccag
    16261 ttcctcaata ttcgtgactg gccgtttggt gcagccacca gcattacgct gactatcgta
    16321 atgggcctga tgttgctggt ttactggcgc gcttctcgtc tgctgaataa aaaggtggaa
    16381 ctcgaatgat cggtcgactg cttcgcggcg gttttatgac cgctatctac gcgtacctgt
    16441 atatcccaat cattattttg attgtgaact cctttaacag ctcgcgcttt ggcatcaact
    16501 ggcagggttt taccaccaaa tggtatagcc tgctgatgaa caacgacagc ctgttacagg
    16561 cagcgcagca ttcactaaca atggcggtgt tttcggcgac atttgctacg cttatcggtt
    16621 cactgacggc agttgcgctg taccgttatc gctttcgtgg taagccgttc gttagcggaa
    16681 tgctgtttgt ggtgatgatg tcaccagata tcgtgatggc gatttctctg ctggtgctgt
    16741 ttatgctgct gggtattcag cttggcttct ggtcgctgct gttctcgcat atcaccttct
    16801 gcctgccgtt tgtggtggtg acggtgtatt cgcgcctgaa aggttttgac gtgcggatgc
    16861 tggaagcggc gaaagatctc ggtgccagtg aatttaccat tctgcgaaaa atcattctgc
    16921 cactggcaat gccagcggtg gcggcgggct gggtgttaag ctttaccctg tcgatggacg
    16981 atgtggtagt ttcttcgttc gtcaccggac caagttatga aattctgccg ttaaaaattt
    17041 attcgatggt caaagtcggc gtatcgccgg aagttaacgc gctggcaacc atattactgg
    17101 tgctgtcgct ggtgatggtt attgccagcc agcttattgc tcgtgataaa acgaaaggta
    17161 acacagggga cgttaaatga aaaaatggtc acgccacctg ctcgcggcgg gtgctctggc
    17221 actgggcatg agcgccgctc acgccgatga caacaacacg ctgtatttct acaactggac
    17281 cgagtacgtg ccgccaggac tgcttgaaca gttcaccaaa gaaaccggta ttaaggctat
    17341 ctattcgact tacgagtcga acgaaaccat gtacgccaag ctgaaaacgt acaaagacgg
    17401 tgcctatgac ctggtggttc cttcaaccta ttacgtcgat aaaatgcgta aagaagggat
    17461 gatccagaag atcgacaagt cgaagttaac gaacttcagc aatctcgatc cagacatgct
    17521 caacaagccg tttgacccga ataacgacta ctccattccg tatatctggg gtgcgacggc
    17581 gattggcgtt aacggtgatg cggtggatcc taaatctgtc accagttggg cagatctgtg
    17641 gaagccagag tacaaaggta gcctgctgct gaccgacgat gcccgtgaag tgttccagat
    17701 ggcgctgcgc aaactgggct actccggtaa caccaccgat ccgaaagaga ttgaagctgc
    17761 atataacgag ctgaaaaaac tgatgccaaa cgtcgcagcg tttaactccg ataacccggc
    17821 taacccgtac atggaaggcg aagttaacct cggcatgatc tggaacggct ctgctttcgt
    17881 tgcacgccag gcgggtacgc caattgacgt ggtgtggccg aaagaaggcg gcattttctg
    17941 gatggacagc ctggcgatcc cggcaaatgc caaaaacaaa gaaggcgcgc tgaaattgat
    18001 caacttcctg ctgcgcccgg atgtggcaaa acaggttgcc gaaactatcg gttatccaac
    18061 gccaaacctt gcggcgcgta agctgttaag cccggaagtg gcgaacgata aaacgctcta
    18121 cccggatgct gaaaccatta aaaatggtga atggcagaat gacgttggcg cagccagcag
    18181 catttatgaa gagtattatc agaagctgaa agcaggacgt taattcagca aacgggcggt
    18241 ggtattaccg cccgtttttt agttagcaag gatgtggcta atgagtcagg acagtaaagt
    18301 tttatcccgc tctttcgtcg gtattgtggt ccccttgtag gcctgataag cgtagcgcat
    18361 caggcaatgc ttcctgcttt ataatccctt cagcaacttt tcaacaaact caggcaccac
    18421 ctggcttgcc gggccgtaat atttctcggc aaattcatta ccgacctgac ttggttcgag
    18481 attcagttcc acggtgtgcg cgccatgcag tttcgcttcg tgaacaaacc cagccgccgg
    18541 ataaacatgc ccggaagtac caatggcaat gaaaatatca gccatcgaca acgccatata
    18601 aatttcatcc atgccgagtg gcatttcgcc aaaccaaacg acgtgcgggc gcaagggtgc
    18661 cgggaactgg caacaatggc atttatcttc tggcgtaacg tctccggtcc agtcgagaac
    18721 ctgaccactt tgcgaacaac gaactttcag caattcccca tgcatatgaa tgacattggt
    18781 attacctgcg cgttcatgca ggttgtctat attctgcgtt accagcaaaa agcgatcgcc
    18841 gagggcgtct tgcagtttag ccagcgcaag atgcgcggcg ttaggctgaa tttctggctg
    18901 ctgcagctgt cgacgacggg cgttataaaa cgcttgcacc agttcaggat cgcgatcgaa
    18961 accttccggc gttgccacat cttcaacccg atgttcttcc cacaggccat ctgcggcgcg
    19021 aaaggtacga atacctgatt ccgcagaaat tcctgcccct gtcagtacga gtactcttgg
    19081 tttttccatt gcttccggca ccactttatc tctgaaaaaa atacgctgac gcaaacgctc
    19141 gcgcaggcgg cgtttatttt tacgaaaacg acttaaccga tgaccccgac gcgacagcat
    19201 agcaacctct gttgtttaat cggttagatg taggaaggcc gcaccacgca ttcctcccgc
    19261 atcaccgtgg cgcgcgcgtt caatgcgcgg aacacgagct acaggtaaga gatgacgagg
    19321 cagcctgtcc gccagttgcg ttgtgattgc cgggaaattc gataagccac caccaatgac
    19381 gaccaggtca gagtcaacga tggtcaggat atttcccaga caaaccgcta ataaatccag
    19441 ataacgctca acgtgcgccc ttgcctgctc atcgccttga tcataaagcg caataatttc
    19501 gggagcctgc aacggttgat gataatagtg ttgccagagc cacgcaaagc cccgacctga
    19561 tagataattt tcgatacaac caatttgacc acagccgcag cggcgtaacg ggaaatccag
    19621 ccccatcatg gttaacgcat caaccggcag acgcatatgg ccaaactcgc cggtaatgta
    19681 gcttttaccg gtaatcggtt taccgttgaa aatcagcccg ccgccaacgc cggtgccgag
    19741 aattaacccc atcaccagtg gatattgcgt aaattcatca tcccaggctt ctgaaagggc
    19801 aaaacagttg gcatcgttat caaggcgtac atcgcgatca agacgtgcgc tcaggtcggc
    19861 acgcagcggt ttaccgctgg cagcggggac attggcggca tacagcgtac catcttctgt
    19921 ttccggcatt cccggaatac cgatgccaac agaaccttta cagccaaaac gttggtcagc
    19981 ttcggctacc agctcgcata ctgcatctaa aaatgcgtca tagctgtcac gcggtgtcgg
    20041 cacccgcttt tcccactgca actgccgacc gctatcaaac acgccaagcg caatttttgt
    20101 tccaccaata tcaaacccgt aatacattgc cgctccttgt tttaatgtac tgcctttact
    20161 ggccgctaag gactcgcgca gggtcaatat tactggcgcg ccgcgccgga taccaacttg
    20221 ccaaaagact cagcaacaat gctgtgacca gtacgtagaa gacgtccagc cagtgcaatt
    20281 ccgatggcag gaagtcaata aaatagatat cgctggagag gaactgatga ccgatcagct
    20341 tttcaatccg ctcaataatc ggggtaagtt gcagtgaaac aactacgccg ataatcacac
    20401 cacacaggct accgaacagc cctgccagca atccatacca gacaaagatg gcgcgaatta
    20461 aaccatcttt cgcccccagc gttcttaata ctgcgatatc gccactcttg tctttcaccg
    20521 ccatcactaa ggtggagacg atgttgaaac aggccacgcc aatcaccagt accatcgcca
    20581 gatacataat ggcgcggatc atctggatat cgcgatacat atagccgtaa gtaccaatcc
    20641 aacttttaat ataaacatag ctgttggtca cttcacccgc atcgcgtacc agcttattgg
    20701 cgttgaaaac atccgtcatt ttaagggcaa tacctgacac gctggaaccc atatcaagat
    20761 attgttgggc atccgccagc gggatcatgg caaaactgtg atcgagttga ccactcaact
    20821 gcaaaatacc ggcaatgtgc aaacgcacac gttttggctg catcagttta tgctcgggat
    20881 tcgagttggg aatcatgatc gacacccaat cgccctgctt cactttcagc gcatccgcca
    20941 cgcctttgcc gatgataatt tgctgttcgc ccgctttaaa attgcgccag gcatccccct
    21001 gaacaaacga gggtaatgcg ctcagacgct gttcctgttg cgggttaacg cccttcacct
    21061 gaattgcgcg cagattggta ccactttcta ccagtccggt gaaattgata tacggcgcag
    21121 cggcggcaat acccggcact ttttgcacgt tatccagtgc ttcctgccag ttagtccatg
    21181 gctgatttac cgcctcaatt tcaccgtgcg gtaccacagc aagaatgcgg ttattcagtt
    21241 cgcgttcaaa gccgttcatc gcgcttaagc cgacgatcaa taccgccacg ccgagggcaa
    21301 tgccaatggt agaaatgaca gagattagcg acaccatgcc gccgcgtcgc cgtccgcgac
    21361 taaaacgcag gccaatcaat aacgataaag gcatcgccat tactccgccc ccatcaggct
    21421 cagttccgcc gtcagacgtc catcacgcat ttccagttgg cggctcatac gtttcgccag
    21481 ttgcaggtcg tgagtaacca ccaggaaggc ggtgccctgc aagcgattca attccccaag
    21541 caactggaag atgctgtcgg cgttacgcgc atcgaggtta ccggtaggct catccgcaag
    21601 taccagacgc ggattattga ccagcgcacg ggcaatcgcc acacgctggc gttcgccgcc
    21661 agaaagttca gatgggcggt gattcgcacg atgctccagc cccaccgctt ttaacatctc
    21721 cagcgcacgg ctgttgattt cagcgggctt tttcttgcca atcagcagtg gcatagccac
    21781 gttttccagg gcagtaaaat ccggcagcag gtggtgaaac tgataaataa agcccagctt
    21841 ctggttgcgc agttctgctt tcgccgccga agacagtttg ctcatcggtt gaccgttaaa
    21901 gatcacatcg ccggaggttg gtgtatccag cccgcccagc aggtgcagca aggtactttt
    21961 accggaacca gagctaccga cgatcgccat catttcacct tcgccgacgc tgaaactgac
    22021 attgtgcagt acatcggttt gcacactgcc ttcctgatag cgtttgcaca ggttgtcgca
    22081 ttgcaacagg atcttattca taacgtaaag cctcagcggg ttgagtggcg gcagcgcgcc
    22141 atgaagggta aagcgtagac agcagcgcga tagccatcgc caccagcgca ataacaatga
    22201 cctgtaaagg ttcgatagcg accggcagcg ccgcgccatc aagcaggacg ccgattatcg
    22261 gcatcagatt atttaactgg ctggcaagca gtgcgccaag cgccgctccg aggatcgcac
    22321 cgataatccc ggcgctggcc ccttgcacca taaagaccat catgatttgt cgcggagtta
    22381 agccttgcgt ttgcaggatc gcgacttcgc cctgcttctc cattaccatc agccctagtg
    22441 aggtaataat gttaaacgcc gcaacggcga caatcaggct cagcagcaag cccatcatat
    22501 ttttttccat gcgtacggcc tggaacagct cgcctttacg gtcacgccag tcctgccatt
    22561 tgctgccttc aggcagtttt tgctgactta aagagtcaac tttcagcggc tcatccagcc
    22621 acaaacgcca gccggtaata ttgcctgccg gataacgcat cagacgcgag gcatcctcaa
    22681 tattcaccag catttcatag ccatcgactt cactgttggc ggcgaaattg ccaatcacat
    22741 tgaacaggcg ctggcttgga atacgcccca tcggcgtgaa ctggctggca gatggtacca
    22801 tcacgcggat ttgatcaccg cgattaacgc ctagctgtga ggcaagttgt tcgccgagga
    22861 tgacattata tttccccggc tcgaggtcag tttgtttcac attgaccaga tacggcgtaa
    22921 gtggatcttt ttgcgccggg tcgataccga gcatcacccc gaccgccacg ctgcgcgcgc
    22981 tttgcagtac cacatcaccg gtagtaatag gtgcgacgcg attaacgccg tccagtttga
    23041 ctgccgtttc cgggagttgc tgcgggttaa gagagccatg ctcagaagag agaattgcct
    23101 gtggcatcag gccaaggatg ttgttttgca gctcgcgctc aaagccgttc atcactgaca
    23161 atactgtgac cagcgccatc accccgaggg taatgccgat ggtagaaagc caggagacga
    23221 aacgaccgaa gcgatccgct gcacgcccac gcatgtaacg caggccaata aatagagcga
    23281 caggttggta catgaaatcc gtctggttgc tgtagcaaag acacgagtat ataagcgaaa
    23341 gcaagatggg taaacgtctg aactgtaagt gatcgtcagg gctaatctga aaagcccccg
    23401 taaatcaatc ggctaaattg ccatgtttta tcatcaatct tgccgcctga tcttaatctg
    23461 aaaaatcatc caatacagac agatacccat tacacctcgg gtcataaccc gcaggatcaa
    23521 ccgcatgtaa attaaaaaag agcgtgttgt cctgatgaaa caaaaagagc tatggattaa
    23581 ccagatcaaa gggttatgta tttgtctggt ggtgatttat cactcggtca ttacctttta
    23641 tccgcatatg accacttttc agcatccgtt atcggaagtc ctgagtaaat gctggatcta
    23701 tttaaatctt taccttgcac cctttcgtat gccggttttt ttctttatat ccggctattt
    23761 gattcgccgc tatatcgaca gcgtaccgtg gggaaattgt ctcgataaac ggatctggaa
    23821 catcttctgg gtgctggcgc tttggggcgt ggtgcagtgg ctggcgttaa gtgcgctgaa
    23881 ccagtggctg gcacctgagc gcgatttaag caatgcctcc aatgccgctt atgccgattc
    23941 caccggtgag ttcctgcacg ggatgatcac cgccagcacc agcttgtggt atctgtatgc
    24001 gttaattgtc tatttcgtgg tatgtaaaat ttttagccgt ctggcgctgc cactattcgc
    24061 cttgtttgta ctgctgagtg tggcggttaa tttcgttccc acgccgtggt ggggaatgaa
    24121 cagtgtgatc cgcaatttgc cttattacag ccttggcgca tggtttggcg caacaataat
    24181 gacctgtgtt aaagaggtgc cgttgcgccg ccatctgctg atggcttctt tgcttaccgt
    24241 tctggcggtc ggtgcctggt tgtttactat ctcgctgctg ttgtcgctgg tatcgattgt
    24301 ggtgatcatg aagctgtttt atcagtacga gcaacgattc ggtatgcgct ccaccagcct
    24361 gctgaatgtg attggctcca acaccattgc tatctacacc acccatcgca ttctggttga
    24421 aatattcagc ttaactctgc ttgcgcaaat gaacgcagca cgctggtcgc cgcaagtcga
    24481 actgacactc ctgctggttt atccctttgt cagtttgttc atctgtactg ttgcgggctt
    24541 gctggtaaga aaactttcac agcgtgcatt cagcgatctg ttgttctccc cgccttcact
    24601 gcccgctgcc gtcagttact ctcgttaaac catcgcccca gccggggcga ttcaatttgc
    24661 taaaccatgt cgattacgga taatgaacag cattgcttat cagagatatg cccccatatg
    24721 ttgaggcata tcctaacgag aatctgacaa ccgttatgcc tgaacaatat cgttatacac
    24781 tgcccgtcaa agcgggtgag cagcgtctgc tgggcgagtt aaccggcgca gcctgtgcaa
    24841 cgctggtagc ggaaattgcc gaacgtcacg ccggtccggt ggtactcatt gcaccagata
    24901 tgcaaaatgc tctgcgtttg catgatgaaa tcagccagtt caccgatcag atggtgatga
    24961 atctggcgga ctgggaaact cttccctacg acagtttttc gcctcatcag gacattatct
    25021 cctcgcgcct ttccaccctt taccagctac cgacgatgca gcgtggcgta ctgattgttc
    25081 cggtgaatac gcttatgcag cgcgtttgcc cacacagttt tctccacggt catgcgctgg
    25141 tgatgaaaaa aggtcagcgc ctgtcacgag atgcattacg aacccaactg gacagcgccg
    25201 gttatcgcca tgttgaccag gtgatggagc acggcgaata cgccacgcgc ggcgcgttgc
    25261 tggatctctt cccgatgggg agtgagctgc cttatcgtct tgatttcttt gatgatgaaa
    25321 tcgacagcct gcgggtgttt gacgtcgaca gccagcgcac gctggaggaa gtagaagcga
    25381 tcaatctgct gcccgcgcac gaatttccga ccgataaagc ggcaattgaa ctgttccgca
    25441 gccagtggcg cgataccttc gaagtgaagc gcgatccgga acatatttac cagcaagtga
    25501 gtaaaggcac attacctgcc gggatcgagt actggcagcc attgttcttc agcgaaccac
    25561 tgccgccgct gttcagttat ttccctgcca ataccttgct cgtgaatact ggcgatctgg
    25621 aaaccagtgc cgaacgtttc caggctgaca cgctggcgcg ttttgagaat cgcggcgtcg
    25681 atccgatgcg cccgctgttg ccaccacaat cgctctggct gcgggtggac gagctcttct
    25741 cagagctgaa aaactggccc cgggtgcagc taaaaactga acatttaccg acaaaagccg
    25801 cgaatgccaa tttaggtttc cagaaactgc cagacctggc cgttcaggca caacaaaaag
    25861 cgccgctgga tgcgctgcgt aagttcctcg agactttcga cggtccggtg gtgttctcgg
    25921 tagaaagtga aggtcgccgt gaagcgctgg gtgaactgct cgcacgaatt aaaattgctc
    25981 cgcaacgcat tatgcgtctt gatgaagcca gcgaccgtgg gcgttatctg atgattggcg
    26041 ctgccgaaca tggttttgtc gataaggtgc gtaatctggc gctgatctgc gaaagcgatc
    26101 tgctcggtga acgtgttgcc cgccgtcgtc aggattctcg ccgcaccatc aaccccgata
    26161 cactgatccg taaccttgcg gagctgcata ttggtcagcc ggtggtccat ctggagcacg
    26221 gtgtcgggcg ctatgccgga atgaccacgc tcgaagcggg cggcattact ggcgagtatt
    26281 tgatgctcac ctatgccaac gacgccaaac tgtatgttcc ggtgtcgtca ctgcatctga
    26341 ttagccgtta cgcaggtggc gcggaagaaa acgccccgct gcataaactt ggcggcgatg
    26401 cgtggtcacg cgcgcggcag aaagcggcgg aaaaagtgcg tgatgtggcg gcggaattgc
    26461 tggatatcta cgcgcaacgc gccgccaaag agggcttcgc gtttaaacac gatcgtgagc
    26521 agtatcagtt gttctgcgac agcttcccgt ttgaaaccac gccggatcag gcgcaggcca
    26581 ttaatgcggt acttagcgac atgtgtcagc cgctggcaat ggatcgtctg gtgtgcggcg
    26641 atgttggctt tggtaaaaca gaagtggcga tgcgcgccgc tttcctggca gtagataacc
    26701 acaagcaggt ggcggtgttg gtgcctacca cccttctcgc gcagcagcat tacgacaact
    26761 tccgcgaccg tttcgccaac tggccggtac gtatcgaaat gatctcccgt ttccgcagcg
    26821 ccaaagagca gacgcaaatc cttgcggaag tggcggaagg gaaaatcgat attctgatcg
    26881 gtacgcacaa actgctgcaa agtgacgtca agtttaaaga tttaggcctg ctgattgtcg
    26941 atgaagaaca ccgcttcggg gtgcgtcata aagagcgcat taaagcgatg cgcgcgaacg
    27001 tggatattct gacgcttact gcaacgccga tcccacgcac gctgaatatg gcaatgagcg
    27061 gaatgcgtga cctgtcgatt attgccacgc cgcccgcccg tcgtctggca gttaaaacct
    27121 ttgtccgtga gtatgacagc ctggtggtcc gggaggcgat cctgcgtgaa attttgcgcg
    27181 gggggcaggt ttattatctc tacaatgatg tggaaaacat ccagaaagct gccgaacggc
    27241 tggcagaact ggtgccagaa gcgcggatcg ccatcggtca cgggcagatg cgcgagcgcg
    27301 aactggaacg ggtgatgaat gatttccatc atcaacgttt caacgtgctg gtttgtacaa
    27361 ccattatcga aaccgggatc gacatcccga cagccaacac cattatcatt gaacgcgcgg
    27421 atcacttcgg tctggcgcag ctgcaccagt tacgcggtcg cgtcggacgt tcgcatcatc
    27481 aggcatatgc atggttgctg acaccgcatc caaaagcgat gactaccgat gcacaaaaac
    27541 gtcttgaagc gattgcctcg ctggaagatc tcggggcagg ttttgcgctg gcaacgcacg
    27601 atctggagat tcgcggcgcg ggtgaactgc ttggcgaaga acaaagcggc tcaatggaaa
    27661 ccatcggttt ctcgctgtat atggagttgc tggaaaacgc cgtcgatgca ctgaaagccg
    27721 gacgcgagcc gtcgctggaa gatctcacca gccagcaaac agaagtcgag ctgcggatgc
    27781 cgtcgctatt gccagatgat ttcattcctg acgtgaatac gcgtttgtcg ttctataaac
    27841 gtattgccag cgccaaaacg gaaaacgaac tggaagagat caaagtcgag cttatcgatc
    27901 gcttcggcct gctgccagat ccggcgcgta ccctgctgga tattgcccgt ctgcgccagc
    27961 aagcgcagaa actggggatc aggaagctgg aaggtaatga gaaaggcggg gtgatcgaat
    28021 ttgccgagaa gaatcacgtt aatccggcct ggttgattgg tttgctgcaa aaacagccgc
    28081 agcattaccg ccttgatggt ccgacgcgcc tgaaatttat tcaggatttg agtgagcgga
    28141 aaacgcgtat cgaatgggta cgccagttta tgcgtgaact ggaagagaac gcgatcgctt
    28201 aatggtaaat cgggggcgtt tctgcgcccc catctgcaac atttacaaat tctttgcact
    28261 tccctgcact atccgacact gtcaccatcc ataattcagg cttatctgtt tattacaata
    28321 accttatatt tattattgat ttttggtgat gatcaaaacg cgtttttctc gctggctaac
    28381 gttttttacg ttcgccgctg ccgtggcgct accggcaaaa gccaacacct agccgctgcc
    28441 gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg
    28501 ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa
    28561 ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg ttaacgatcc cgttgcaaac
    28621 cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac attgcggagc tgcgtctcta
    28681 ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg
    28741 tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac
    28801 ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctac
    28861 ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta
    28921 tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag
    28981 ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc
    29041 aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc
    29101 gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct
    29161 gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga
    29221 agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca
    29281 agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg
    29341 cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact
    29401 gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc
    29461 atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta
    29521 gcactgatag taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca
    29581 aagttggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc
    29641 ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa ttacttaacg atcgataact
    29701 aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc attaattaat
    29761 ttaaaaatct ttaaatttca gttaaataga aagcaattat ttttatggga tctggactgg
    29821 tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg ttcaacggca
    29881 tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg
    29941 acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga
    30001 cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat
    30061 ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg
    30121 ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact
    30181 attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc tgcttcatga
    30241 tcaacaactg caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac
    30301 gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg
    30361 aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg
    30421 ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actgatgcag attgccggaa
    30481 cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg
    30541 agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag
    30601 ttgcccgtta ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc
    30661 ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata
    30721 caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg
    30781 gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc
    30841 acgaccacca ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc
    30901 ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc
    30961 ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg
    31021 cgggccccgt tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac
    31081 accaatcccg atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt
    31141 atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg
    31201 aatggctgga aatacttaag gattcggaga agtctgaagg aatgcctgat gcgacgctta
    31261 acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc
    31321 cacatccgcc agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa
    31381 tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca
    31441 ttcggtatag cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc
    31501 gcgtcaggtt acccatcagg ctaccgacag tggagaagtt cgacagcgat accagcgaac
    31561 catgatcttt atactgataa tttttcagcg gtttaccgtt catctgcgcc agaatatttt
    31621 tcattgcgca ggtcgccatc tggtgtgcag cctgagcacg cggcggaaca aagccccctt
    31681 ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt
    31741 gcagcgtcgg ttccaccacc agctggttga tacggttggt ttcaagacca ccgatatctt
    31801 tcaggaagtc tggcgctttg atcccggctg cccacaccat cagatcagcc tcaatatatt
    31861 caccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagca
    31921 cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc agagatacgt ggcggtaatg
    31981 caggcaaaat acgttctccc gcttctacca gcgttacgtt cagggcttcg ttggtcaggc
    32041 ctttgtaacc gtagctgtgc agttgcttga ccgcattgta caattcagcg gagagttcta
    32101 cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt accattcgcg cccaggttgg
    32161 cggagtattt caggaacaga ttcagcatct cctggtggaa gcgacgcgcc tggtgcgggt
    32221 tatcgaggaa aatgcagttc tctttgacac ctggcgtatt gaaatcgtta gaggtgctac
    32281 ccagcgccat taccagggtg tcataggcga ttttacgttc cggaaccagc agttcacctt
    32341 tctcatcgcg cagttctgca atagtgattg ttttcgcttc acgatcaata tcaatgacgg
    32401 aacccagctg gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga
    32461 cgccttcatc aagcgagcca gtcgccactt cgtgcagcag cggtttccac agatggctgt
    32521 ggttacgatc gaccagcgta attttggctt ttttcttgcg tcccagctta tgccccagct
    32581 gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaattttt ttcaatggcg
    32641 tagtcaacgt gaccccctta aatttattaa ccaattgtta attaatggtt ataaacatag
    32701 ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga
    32761 atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc
    32821 gactttaccg ggtgagatca aaaaacagag tttattgttt attatttgag caagtccgga
    32881 agggtgatat agggtgacgc gtatatcgag ggcgtttggt cgtcctgata agatgcgcag
    32941 catcgcatca ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt atccgactta
    33001 cagaattcgg agcgcatcag gtaccggcaa ggttacctga tgcgacgagg gcatttaccc
    33061 gagggttttg aacgccttaa tgcgctgcaa atgcggggag atattcttga atttgtgcgt
    33121 ctgctcttcg tcccagacaa tctcgtaata atgatgcaac tcttcagatg tccgctggct
    33181 gttaagcgct tcatcattac gcgacaaaat caccaggcaa cgatcgcgat tcttctcgcg
    33241 gaagttggtc acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc
    33301 ttccatgttc tcataaggga acaaattagg gttgaagatc acctggcgga tatcgcaaag
    33361 aaaaccaatc cgttccgccc agtatccgcc cagaccaacg ccgcaaatca gcggacgctc
    33421 gtcgacgttc agttgcaaca ttttgtccac ttctttgagc agatgctgca tatcatgttt
    33481 cggatgccgc gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa
    33541 gactttctcg tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc
    33601 ttcctcacat aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcatgtaact
    33661 gattcagacg ggtgctgatc gccttccagc gagccgagtc catcagttcc tgtcgcgaaa
    33721 atgaaccttt atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta
    33781 acacgctgac ggccccttta cgattattgc agaccaggat catatcgcaa cctgcgtcca
    33841 gtgatgcctg cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat
    33901 cgtcagagaa aattacaccg tcaaaacaca gttcctgacg caaaacggtt ttcagccagt
    33961 aggaagaacc gctcgccgga cgcggatcaa catcactgta gatcacatgc gcaggcataa
    34021 tggcgtcgag tttattttcg cggattaagg aactgaagac cgacatatct ttagcgcgaa
    34081 tctccgcttg tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc gttactgcac
    34141 cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc ttcatgcata ccatcaataa
    34201 accggctggc aattgccagg gctttttgtg gatcggcatg ataagaacgc tcgccaatcg
    34261 ccgcgctgat atgcccgaca tccagcactg gcgcaaagct gatatcaata tccatagcga
    34321 tcatttcgct ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc
    34381 ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct
    34441 gcacgcgtcc accttcctga tctaccgcca ccaccagatg attgcgcgat gctgcgcgga
    34501 tctggcgcac cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca
    34561 gccctcccac cagcggatgc gccagtattt cacgctcttc cgcgtccagt tcgtaacctt
    34621 cgacatccaa cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc
    34681 caggtgtcat cggccagcct gataaattgt tgatcgccga tttgtcgcca gcggtactca
    34741 aaccaccctg ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat
    34801 tgcgccggat aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca
    34861 gtattttcca cccacaccgc cgccagttcc agcgcgatat caccatctcc ggcatactcc
    34921 cagtcgatga gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg gacatccata
    34981 tgcaatggac ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt
    35041 cgcagccaac ccacagtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaag
    35101 ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat gaagataata cagcaagcct
    35161 gccagttcgt tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc
    35221 atccagtcac ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa
    35281 gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca
    35341 acaaaacgct ggccctgatg ttcaatgaga aaactaccgc cactaagccc actattaaac
    35401 gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga
    35461 ttattgctgc gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat
    35521 cagctgcatt tgtagggtcg gagcgttaac gttgccagag gcgctggagt acagcacgta
    35581 atgagcgccg acattgcggg caatgcctat ggctttacta cgggtaccta aactgtcctg
    35641 cggcgacaaa cctaactgtt gcttagccat cgacagctgc tgggcggaaa ccagggtaaa
    35701 tttcccgtta ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga
    35761 accgttagta cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc
    35821 agccccaagc atcttactga ccatcggctg cattgcgcca ttccagtcat aatggcgaat
    35881 atgcggcgca ggcggtgccg tttgatcttc gtgctcaatt gggcctggct gctgcgggat
    35941 cgtcggcacc gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc
    36001 tggtttcact tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat
    36061 cgccagcgcg gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca
    36121 aatatagata aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta
    36181 ccgccgaatg tgcgggaatg ctaacgctgc gtggcctttc cagaggatgc atctccagcc
    36241 ctctggcgtc ataccagtaa aaacgataat gaacggtaac gggttcttgc ctttcgttat
    36301 aaagcgttga ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac
    36361 tgatgcccgc agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa
    36421 tttccggatg tgaacgacag cccaccagca gtaacaacgc cagagacacc agcccaaagc
    36481 atccttttct catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc
    36541 caacaagtgc atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat
    36601 cagacgatag ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat
    36661 catgcgtccc agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgaggat
    36721 attcggaatg atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac
    36781 cagatcatcc tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat
    36841 agtttcttct gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc
    36901 cgcgccacac tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc
    36961 acgatttctt aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct
    37021 gcctgcgaat gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgc
    37081 atatttctca tttgcattta caaacaaaat tattcgcaca ctaaatataa ccgctcatca
    37141 ccctcctccg gcatgagcct gacgacaaca taaaccaaga gatttcaaat gctttcaaca
    37201 caatttaaca gggataatca atatcaagcc atcaccaaac cgtcactact tgccggttgc
    37261 atagcactgg cactattacc ttctgccgct tttgctgcac cagccactga agaaacggtg
    37321 attgttgagg gttcagccac agctccagat gatggcgaaa atgattacag cgtaacgtct
    37381 acctctgcgg gtaccaaaat gcagatgact caacttgata ttcctcagtc ggtcactatt
    37441 gttagccagc agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac
    37501 acgctgggga tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgtgga
    37561 ttccagatcg ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat
    37621 ctgggcgacg cactttctga tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg
    37681 acaggactca tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac
    37741 gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa
    37801 cggtatgtgg cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt
    37861 gtcggcggct accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc
    37921 ttctcgggca ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa
    37981 tatcagcgca ttgatgttaa tagcccaacc tggggcggtt taccgcgctg gaatactgat
    38041 ggcagcagca acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat
    38101 aaagagatca acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctagcaagcg
    38161 acactgaatg ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc
    38221 tatgtaaaca aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt
    38281 gattatgtcg gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg
    38341 ttcgctgacg gtagttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt
    38401 tacagcaaac aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt
    38461 ggcagtttct acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg
    38521 gcacaggacg ataccacaca tatgaaatcg ttatatgctg ctactcgagt cacccttgcc
    38581 gatccgctgc atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact
    38641 tacagcatgg agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac
    38701 aactggtcga cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt
    38761 tcaggcaaat atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac
    38821 tggatgaata gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc
    38881 gctcagtcca ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg
    38941 gatgggacag tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg
    39001 cagctgacat ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat
    39061 cctaatctgc cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca
    39121 gagttgacag tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca
    39181 ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc
    39241 taccaggtga cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc
    39301 tacgatacca acatggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc
    39361 ggcacgtatc aattctgaat aacacctgta aaaaaggcag ccatctggcg gccttagtct
    39421 ccccaacgtc ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta
    39481 tcggatttag taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg
    39541 cccagtttct tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga
    39601 cgggtaatac atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga
    39661 gccggtgcca tttcgctggt acctgttgct tttgcatctt cagtcgcgtc ttcacgaccc
    39721 ggcgttttca gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca
    39781 taaccgatac cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgata
    39841 aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt
    39901 gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg
    39961 aaggagaact cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc
    40021 ataataccgc ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg
    40081 gcagctggca gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg
    40141 tcacccgcca tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat
    40201 tcaccaatct gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggcaccagg
    40261 caacgttcga tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac
    40321 tgagagaagg tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca
    40381 gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca
    40441 agatactcag gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg
    40501 gagataatcc ctccgagtac gccagtatcc gccaggtgtt tagaggcgat ttcttcagca
    40561 ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca
    40621 acaactgcgg ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc
    40681 gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat
    40741 acaacggcgg gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata
    40801 ggcagtacgg ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca
    40861 ttcttaaaca taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt
    40921 ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca
    40981 gagtaaaata attcagtgcc gatttgtttg acggctatca catttcaact tcagatcatt
    41041 ggagaagttg cacattttga caaaacatat gtcacaatat ttcaaacgtg gttattcacc
    41101 gcccaatttt cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa
    41161 ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta
    41221 tcggtggtta cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct
    41281 gcaacgtcac gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg
    41341 taaggtgagt cagtttccac cagtaaccga tccaggggga cataacgcgc agcatctcga
    41401 agttgctccg cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc
    41461 agtaatttac ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc
    41521 gtcacttttt cttcgcgcag aattgccagc gtatcggcgc gggcgtcacg ggtatggacg
    41581 ataaccggct tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga
    41641 cgaactttag tttccggtgt gtaataataa tccagcccgg tttcacccag cgctacaaca
    41701 ccctcttctg ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt
    41761 ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt
    41821 aaataacccg gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct
    41881 ttcgccagaa cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg
    41941 cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac
    42001 gcccggttgc aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt
    42061 gatgcctgtg acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg
    42121 caggcgcgag ggagaaagat ggtttgccag ttcgacgaca aggcccggca catcaacatt
    42181 ggtcacctgc gcagcaccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca
    42241 gtgtaaacgc gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc
    42301 cgatggcacg ctatatgcca atgcctgaca caatgtttca cgagcctgcc agttatctcc
    42361 ctgaaacaac gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa
    42421 tgcatcctgt gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg
    42481 cggcgcaagg taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc
    42541 gcgggtagcc aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc
    42601 gttagccgcg gcgtcggtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc
    42661 taggcgtgcg tgctcattca gcttttcggt gacctcacgt actgcatcaa tgcccagcgt
    42721 atttttccct ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa
    42781 ctgacatcca cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata
    42841 acggctcagg gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag
    42901 cgcatggtga cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa
    42961 ccatggatac catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt
    43021 gcatccatca cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt
    43081 gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca
    43141 atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg
    43201 agatagagcg ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc
    43261 atatgttggt caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga
    43321 tcgccaatca cccaggtgcc gttagccaga gctggtttga tgaccgtttc taccagttga
    43381 acgcgcgcgg cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc
    43441 gatttgatat ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc
    43501 cgagtgaaaa ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac cacattacgc
    43561 gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt
    43621 ttccttaagc actttcagat aatcctgcac agacttgtta tggctggcaa gattggtatt
    43681 aaacgtgtga ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg
    43741 cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt
    43801 aatggtatag gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt
    43861 ataacgctct cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg
    43921 taaacggttg ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt
    43981 ttcgataatt gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc
    44041 acgcccttcc caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa
    44101 cgcgacatcg gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat
    44161 ccactccggg ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag
    44221 cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc
    44281 ttctaccagt cgcagcggga actgtgcttc tttaccgctt tccagcaatt tcagcatctc
    44341 gcgcacggtc atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg
    44401 ttcgatacgc agcaaccatt gaaaaacccg tgggcgattg atgatcttat cggcataaag
    44461 ctgttcacca agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt
    44521 gataagcaat ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat
    44581 acccagtacc accagcaata acaagataat caataacact tttttcatga ctaattcggg
    44641 cgctcacaaa gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca
    44701 caggcacgta cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc
    44761 aacgcctctt ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa
    44821 cggatacaga attgtcgcat aatgccgtta acacctgcct gatccagacg cggcgtataa
    44881 actacattgc ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg
    44941 ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact
    45001 tgctcaagcc gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc
    45061 gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc
    45121 gtaacggaga gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca
    45181 ctaccgcgac tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc
    45241 atctcttgtt caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc
    45301 tgtagtcgct ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg
    45361 gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta
    45421 tgaccgttaa ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt
    45481 tcactacgga acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat
    45541 atgggtggga aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga
    45601 agccgaagga gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta
    45661 cgaaatccag atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct
    45721 gatcgcgcag cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt
    45781 gaccggtcat agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg
    45841 ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa
    45901 cgtagccaat ctgactcgct tcaatgcctg catcacgcag agcatttgcc atcgccagag
    45961 ctgcgcctgc gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa
    46021 agccgacgag ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa
    46081 gtaccagcat accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc
    46141 ggctcgccgc ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac
    46201 caacacccag cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc
    46261 catacgcgat aatacgcgca gcatggccaa tgttgtgcac gccggaagta caggcggtcg
    46321 cgatagagat gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt
    46381 tcacaatcgt tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag
    46441 atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa
    46501 tgcgggttgc gttctcttcc gttatatcaa ggccagaatc ctgcatggcc tgaacgccag
    46561 cgacaattcc atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt
    46621 cctcacagtt aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga
    46681 aatggtcgat taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta
    46741 cggtattgcc gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca
    46801 cgtttgtcct ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc
    46861 ggtcgaacga ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg
    46921 cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt
    46981 cttccagagc cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga
    47041 aagaagcatt gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct
    47101 taacgcgttc ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga
    47161 tggttttcgt agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc
    47221 acgattttac gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt
    47281 attactcata accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga
    47341 ccatgtacat cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg
    47401 atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa
    47461 cctgcgccag gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa
    47521 taaagcccgg agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg
    47581 atttactgaa gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc
    47641 ccatggtacc aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag
    47701 cgcgcattac cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt
    47761 tccactcttc atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga
    47821 ccaggatatc cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg
    47881 ccgggtcggt cacattcaac atcagacctt tgccgttggc acctaaataa tcgctgatcg
    47941 cctgagcgcc attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg
    48001 tttcagcaat tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt
    48061 caaaattcat gattttcctc ttttaaagct caagcgccgc tgccatcgct gaaggttcgt
    48121 tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc
    48181 ccgggccgac ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg
    48241 tccactgaac cgggttatac aactgacgta ccagtgcgtc acggatggca tcaccattgg
    48301 tttcgcattt cacatcaacg ttattcacaa cagaaactgt tggtgcgtta aaggtgattt
    48361 tcgctaattc tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta
    48421 cgctcactgg taacggcaga gcacgttttg cgcccgcagc tttacaggca gcgccagcac
    48481 gctcaaccgc ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg
    48541 gagaaacgac ctgacctgct gcagcttctt cacacgcttt cgcaatagac gcatcatcca
    48601 gaccgatgat tgccgccata gcgcccgtac cttccggtac ggcttcttgc atgagcttgc
    48661 cgcgcatctc aaccagacgc accgcatcag cgaaatcaat cacaccagcg caaaccagcg
    48721 cggaatattc ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct
    48781 gccatacgcg gtacagcgca acagaggcag tcaacagcgc tggctgggtt tgccaggttt
    48841 tattcagttc ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg
    48901 cagaagcttc agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca
    48961 ttccaacggt ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat
    49021 ccttatccta gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa
    49081 gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg
    49141 ggacagaggc cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatag
    49201 acataccgag ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca
    49261 gccagtccag ttgagaacgg tcaagattat ttgccgccag cgtctcatca acgatgtgcg
    49321 ccagttccgt taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat
    49381 tctctggatt cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg
    49441 catgcagatg ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg
    49501 cgccatcgcc aaaaataata atagtcccac gatcggttgg atcgcaggtg cgcgccagta
    49561 catcggaacc gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta
    49621 cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt
    49681 taatgcccag catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag
    49741 tcgttgccac aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc
    49801 gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt
    49861 cgcggatacc ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat
    49921 cggcgtttgt ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca
    49981 tgtacgctca gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca
    50041 gcgtcctgct accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg
    50101 tatacagatt ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact
    50161 gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc
    50221 gtgccgcgca atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat
    50281 cgcctcgtca ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt
    50341 ttcccttcac cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc
    50401 tttaatgtga catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac
    50461 aactcattgg cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct
    50521 gaggcatccc gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc
    50581 acgcgaggat tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat
    50641 tgcaccaaca ttgtactgtc acaatcgacg ttggccccta agtcaaggac caccgttttg
    50701 cccttttgct gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc
    50761 ttgagtaata attttgccag ccccatcagt gccccggtat taccggcact gacacaggct
    50821 tgcgctcgac cttctttcac cagctccagg gccacgcgca ttgaactccc acgactggcg
    50881 cggatagctt gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc
    50941 agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg
    51001 ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact
    51061 gtcacggaag ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag
    51121 gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct
    51181 tcatcacgca gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg
    51241 cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga
    51301 ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct
    51361 gttgtacggc catggacctt actcctcaat tacggtttat ccccgatcca acggggagca
    51421 ccaattactt acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcacttctt
    51481 caggcagttc accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca
    51541 ccggaactac cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt
    51601 caccgaattc gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt
    51661 ctgaacgcac aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc
    51721 aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt
    51781 ggttatcgat agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt
    51841 cggcgacgcg ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag
    51901 ccgtacgaac cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt
    51961 aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa
    52021 ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc
    52081 tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa
    52141 aaatatgcct aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa
    52201 actgcaaatc tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga
    52261 atcaccgcga cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg
    52321 ttatccggat catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac
    52381 cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat
    52441 cgtcaccttc tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga
    52501 agtggagcct tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt
    52561 gcgtaaggag catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac
    52621 gctgtttgag cgtttagagg gacgtgaccc taacacgctg gttggtttac cgctaatcgc
    52681 gctgtgtcag atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgct
    52741 ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct
    52801 tatcaggcca accgcccatt atcaggctta tatgcttgta ttagcgcgcg ttacgcagct
    52861 tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc acctcaccgg
    52921 tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc
    52981 ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg
    53041 gatgacccgc atactgtgta tgcacacgga tctgatgagt acgtccggtt accgggctac
    53101 aacgcaccag ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt
    53161 tgccttcctg actgacacgc acgatacgtt cgccgctttg cagaatattt ttcaataacg
    53221 gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt
    53281 tttgcattcc tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg
    53341 ctatcagcaa cacacctgaa gtgtcccggt cgagacgatg aaccagttca aggaaccgcg
    53401 cttccgggcg caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat
    53461 gtaccgccgt accggaaggt ttattcagca ccaggatatg atcatcttca tataagatga
    53521 cgtccgccag cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag
    53581 caacgcgaac cggtgggata cgcacctcat cacccgcttc gagtttatat tcaggcttaa
    53641 tacgtttttt gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg
    53701 gtacgccttt caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg
    53761 tgatagcaac aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc
    53821 caacaggatt cgcgccactc atttttctat gcttatattt agttttgcac cttattactt
    53881 cactacgtga tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac
    53941 tgtgagtaag cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga
    54001 ataatgaggc cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca
    54061 ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt
    54121 tagcgggctg cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc
    54181 cagtctgttt ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc
    54241 tgcgcctctt tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat
    54301 cggttcacac ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat
    54361 gagtaagtta cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt
    54421 gcccttgtag atgggcagcg tctttatgac ctggatatcg aaagtccagg gcacgagcag
    54481 aaaaaggcaa acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct
    54541 tttgttgatt acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa
    54601 tatttccctg ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa
    54661 ggtcaggaag tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta
    54721 accaccttta tcagtctggc gggtagctat ctggttctga tgccaaataa cccgcgcgca
    54781 ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg aattaaaaga agcactggca
    54841 agccttgaac tgccggaagg catggggctt atcgtgcgca ccgctggcgt aggcaaatct
    54901 gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac actgggaagc catcaaaaaa
    54961 gccgctgaaa gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc
    55021 gcattccgcg attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg
    55081 ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc
    55141 aaactgtata ccggcgagat cccgctgttc agccactacc agattgagtc gcagatcgag
    55201 tccgccttcc agcgtgaagt ccgtctgccg tccggtggtt cgattgttat cgacagcacc
    55261 gaagcgttaa cggccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa
    55321 gaaaccgcgt ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg
    55381 cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca tgacgcctgt acgccaccag
    55441 cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc
    55501 agccatattt ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag cccatcactg
    55561 ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac
    55621 gaatcgctgt cgctctctat tctgcgtttg atcgaagaag aagcgctgaa agagaacacc
    55681 caggaagttc acgccattgt tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt
    55741 tctgcggtga atgcgattga aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac
    55801 gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg
    55861 accttaagct acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa
    55921 gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg caacctttgc catgccggat
    55981 gtgccgccag cgccaacccc agctgaacct gccgcgactg tcgtagcccc agcaccgaaa
    56041 gctgcaacgg caacaccagc agctcctgca caacctgggc tgttaagccg cttcttcggc
    56101 gcactgaaag cgctgttcag cggtggtgaa gaagccaaac cgaccgagca accaacaccg
    56161 aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc
    56221 cgtgaccgta atgagcgccg cgacacccgt agtgaacgta ctgaaggcag cgataatcgc
    56281 gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc
    56341 cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt
    56401 cgtgaacgta gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg
    56461 ctgaatgttg aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt
    56521 cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc
    56581 gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag
    56641 gaagctccag ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact
    56701 gcaccagaac agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt
    56761 cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt
    56821 gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa
    56881 ctggcctctg gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaagtt
    56941 gaagagcagc gcgaacagga agaagtacaa gtgcagccga tggtgactgg ggtccctgtc
    57001 gccgccgctg tcgaacctgt tgttagcgca ccggttgttg aagaagtggc tgaagttgta
    57061 gaagcccccg ttcagattgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa
    57121 gtgattgccg ccgcagtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta
    57181 gcccaggaag ttgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat
    57241 attgaagaag ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa
    57301 cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc tgcggttgaa
    57361 cctgaggtca ccgttgagca taaccacgct acggtgccga tgacgcgcgc tccggcaccg
    57421 gaatatgttc cggatgcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa
    57481 ggtaaaggtg ccgcaggtgg tcatacggca acacatcatg cctctgccgc tcctgcgcgt
    57541 ccgcaacctg ttgagtaata attagctcaa gtaatcaagc cctggtaact gccagggctt
    57601 ttttatttca tctttgaatc aacatattaa aattcatcga atggcatcct tgctaaccaa
    57661 caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc cagagcaggc agacaaaaca
    57721 tacccagttt catgatatgt tttaaagcga aattatttgt tgagctggaa gagcgacaat
    57781 ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa
    57841 gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct
    57901 aaagcgcgat cgctacctaa tgaatccagc gactccagtt cgttcagctg cgtgcctaat
    57961 tccgcgcgaa cggtcagcac attgttcagc gagtttttca gtccgcggtt ggttttatct
    58021 aacgccgcag cggcggtttc tttatccgct tcgctatccg cgaccggcgt tttcagggct
    58081 gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc
    58141 gctaccgcgt tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc
    58201 gaacgcgaag catcgacctg ttgcttaata ctttctgttc cgccgacata atcaccgtta
    58261 gcctcactaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg cccattaccg
    58321 tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc cctgaatatc cgtagccagc
    58381 gaggcccggt catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca
    58441 ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg
    58501 gcgaaagtac gcgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaacc
    58561 gcttgtgatg cagcaatggg atcatctgaa gggttaacga ctcgcttacc cgtcgacatc
    58621 tgttcgccgt acttcatcca ttctgcctga gaattggtga taccacgcat gttttgctgg
    58681 tacatcatct gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat
    58741 caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac tgctgaaaac
    58801 gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg
    58861 aaagctgcgt caccacatta ccttgcgtgg tgctactggt tttcaacgtc gcggttttat
    58921 taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg
    58981 ttttactatt gctttgcaga tccagcaggg cctgaccgtt gcggttatcg ctatcacccg
    59041 catctttttc gctcgccatc gctattttcg cttcgtcggt gattaataca tccatgttga
    59101 cgatggcgtc acttactggt ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa
    59161 acgtcaactc cagaccatca aatgccactt taccgttggc atccggcgtc accgtaaaag
    59221 tggtattgct ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat
    59281 ctgtcgccag taccacggag gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt
    59341 ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc
    59401 cgttagcatc aaatccggct ttgtgttggg tgttgaaagc ctcggcaaat gccagcgcca
    59461 gttgtccaag cgtattacgc gtctggtcca gctcctgaga acggaatgtc agaatgccgc
    59521 ccagcgaccc ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa
    59581 cataagcgac agtcgtacga gaagggtcag cgctggaagg aactgccgcc agttgccgcg
    59641 ccgtacttcc ctgaaccagt gagtaaccat tggccaccgt gatgttataa gtgccgccat
    59701 cctgaacgct gacttctaca ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt
    59761 gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc gagatttgat
    59821 cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg gcacctatcg
    59881 cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta aactgattca
    59941 ccaacccttc tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc gcgttactca
    60001 ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg
    60061 tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcggtcagg ccgctacttt
    60121 gcgtctgcgc agcacgtaac tggttggtaa taaacgcatc atactcacgc tgcacaccag
    60181 aaacgtagac gccattgcca acccagccgc cagcgcccaa cgtgctattg gcctgcgcca
    60241 taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta ttacttgccg
    60301 tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc aagctggaca
    60361 tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc gccggacttg
    60421 agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct tgtcgcttat
    60481 cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat cggtagcata
    60541 ccccgcgtcc tgtagggcct gcgccccctg ctccgcactc gcggctgtcg tcacggcggc
    60601 gtagcgcggg ttacgtgtta acagcccaac gtaatccgac aatgcttcca gatacgagct
    60661 gtagacccga aatttcgctt ttactttctt cgcttcgcca ttttcatatt cagtcgtggt
    60721 gatttcagta actggacctt tccagttgcc agaggctttg acgccaaaca ggttatagct
    60781 cggctcgccg ttttcgcggc ggatttgccg ttgcccccaa ccagactcca gcgccgcctg
    60841 agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg cgggcagcga
    60901 gagttgcgcg aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt tacgtggcac
    60961 ggccttttgc accagctgcg aaagcgcctg attttgataa cgcaccacag tttcgagcgg
    61021 gaatttcatc ggtgctgctg gcgtggactc ctctggcaat ggttgttctg gcgtcatctg
    61081 tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt gggcaatctg
    61141 ctggtcatac atactggtat acaggcgagt gtgctcgctg ctgaacaggc catcttttgg
    61201 taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca cctgacgggc
    61261 caccggacgg atatttgccg ccggatcttc gcccgctttc gcctttagtt cgttgagtga
    61321 ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca gatgatttcc
    61381 agttttgccc gcagacatcc cgcactttgc attgattgca gtatggacat cagatccatc
    61441 ggcgtagcgc ccagcgcatt gagcgcgcgc accacgttat tgaggctggc gctggaacgt
    61501 acgctttgca gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg agtaaccaca
    61561 gtctgtccac caccaaacgg tgtatctggc tggctgacat tggcctgacg attgactgtt
    61621 actgagagat ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc
    61681 accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac attaacctgc
    61741 atattctgga tatctgcaag gaaacggacc tgggaactgt tgccacttgg tacgcgcacc
    61801 tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg
    61861 tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc
    61921 ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt gatccgtcca
    61981 ccgttcagtt ggttcacctg aacactgcta ccgccagcgg aggctcctgc gccgccaacc
    62041 agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag cggcgtcatc
    62101 aacaacgtac ctccacgcaa gcttttggca tttcccatgg aagaaaccac cacatcgatg
    62161 gtttgcccct gacgtccaaa cggaggaagt gactctgtca ccattaccgc agcgacgttt
    62221 tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag catgttatta
    62281 agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc cagccccacc
    62341 accagaccat agccaatcag tgagttttgc cttaccccct gaacactggt gagatcgcga
    62401 atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta
    62461 atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgctgc aaccagccca
    62521 tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatgcgcgca tccgccacct
    62581 gagtagacgg tacggtattg ctgccgctga tagtgcgtgg attaaccacg ccagagaagc
    62641 gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca atcaggttgc
    62701 cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat
    62761 tggccccgcc ctttccgttg aacgtgttac caccggaggc ttcgacatcg gcacgagcgt
    62821 taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta gttttaccgt
    62881 cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc aacacgatgg
    62941 tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga tagccatagt
    63001 taatcggctg agcagactgg aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg
    63061 gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg cagccggtta
    63121 gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt tgcatggcta
    63181 tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg ttaagcctta gagttgcgtc
    63241 agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc gtaagcgcgt
    63301 tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac
    63361 ccttgataca gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg tacaccagag
    63421 gattgcgttt cggtgtagag gttttcgcca atgctctcca gcccggtgtc attcataaag
    63481 gtggtgagat tgagctgccc aacctgaacc ggagctgcct ggccttgttg ggttacgctg
    63541 accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt gatcgctggc
    63601 tgcacctgaa aaccaccagc tgcccgttct gatccacctg gaaagagccg tcacgggtat
    63661 aggctgatga accatctggc aacatcacct ggaaaaagcc ctgcccttta atcgcgacat
    63721 ctttgctgtt gttggtctgc gacaggtttc cctggctgtg taagcgttca gtggcgaccg
    63781 ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg
    63841 cccccggctg gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa
    63901 aaccgttagt actgacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg
    63961 cgtcaaggcc cgttttggcg atccataatg aactgatcat aaaatgtcct gtagtggatt
    64021 agtgacaaag tgcatatgtc ttcttgccgg atgcggcgta aacgccttat ccggcctaca
    64081 agtgctcgca aaatcgataa attgcgattg cccccataga cccgataaac ttgcgcatcg
    64141 ggcaacttat acttgtcatg tatcctttca attaactcat cgacagcagt tggttggcac
    64201 ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc
    64261 tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc agaacccccg
    64321 acatcacacg caaggttgga tctgcctgca gtaccggccc tcgcgtggcc tgggtttctg
    64381 cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca
    64441 gtttcagacg ccctactggc gcaactgtat ttgccggatc gcccggattg agcgccgaga
    64501 ttgtgccatc ggcagcaata gtgatttccg ctccttccgg cacagcaatt ggccccgcct
    64561 cgcctatcac ctgatgcccc tgaattgtca gttgcccggt gggatcaacc tgaatgctgc
    64621 cattacgcgt atacccttcg ctgccgtcag cggtctgcac ggccagccag ccatcctgct
    64681 gcaacgcgac gtccagcggg cgcgaggtgt aatccatttt gccgggcgtc atatctgcgc
    64741 ccggcgttga cgccgtgacc aacgtgcgcg tgggcagaga aagcccttcc actggcaccg
    64801 cgcgtaaagc attcaactgc gcgcgaaaac cgggcgttga ggcattggcc agattactgg
    64861 cggttaccgc ctgttgattc agtgtctggc tggctgctcc catcgcggta taaattgcgt
    64921 gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg
    64981 tcctgagttt tgatggtctg ggcgttagac tgatagttac gctgggcaac gatcatattg
    65041 accagttctt tactgagatc gacattggac gcttccaacg caccgttggt cagggtgcca
    65101 aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag
    65161 acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc
    65221 cccagcagtt gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc atcattgatt
    65281 tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac aatattgtta
    65341 gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc gggatctgcg
    65401 ccgttaattg cgccagtggt aatattctct gttggatttg aggtcagaac gccattggca
    65461 ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct atcctgggtg
    65521 tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc atgagcatta
    65581 ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg
    65641 ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc
    65701 gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc
    65761 ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg taaaccttgc
    65821 atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc
    65881 gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct
    65941 cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc
    66001 cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag
    66061 ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg
    66121 tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa
    66181 attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta
    66241 ccgctgttgc cgcggagcac accctgcacc agagcaaact gcagcggctg ggcaaccagt
    66301 tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg
    66361 ccatcagtca acgtaccgtc ccaggtgaaa ctgtggactc cggcggtcag ttcaccaata
    66421 tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc
    66481 gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg
    66541 gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag gttactggcc
    66601 tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt
    66661 tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt
    66721 ggattggtcg ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa actgctttgt
    66781 aagtctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt
    66841 gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa
    66901 gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc
    66961 gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc atttttacgt
    67021 agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc ggggcctgac
    67081 tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    67141 ggaataccac ctgttttgcc cgatatggct gtccatcggg accggtcacg ctatcagcat
    67201 tcgccagatt actggccgcc acgttcaagc gctgggactg ggcagttaac gccgacccgg
    67261 cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    67321 tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg
    67381 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    67441 gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccgga atgtgttgcg
    67501 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    67561 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    67621 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    67681 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    67741 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    67801 atgccgttga tgaccattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    67861 aaatgctggc aataaaacgt agcgtggcga tcatctcgat actattcact ccgttaagtg
    67921 cggcgagtaa tctcacatcg caattgcaaa ccttttttag cgcccagctc gcgggggtaa
    67981 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgccg tgcgagcagc
    68041 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    68101 gcggtaacga caaacgatat ttacaggtta atgtacaggc cacaggaaat tatgtggttg
    68161 ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctaaaacgcg
    68221 gacggcagga taccctgcca ccgcgtacgg tgcaggatat caatcaactt gttgatgccg
    68281 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    68341 ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag cggtgacggg tttagcgcca
    68401 acgcagaagg tcaggcgctg aacaatgcag ccgtcacaca gaatgcgcgg gtgcgcatgg
    68461 tatcgggact ggtggtcagc ggcgttgttg atgcagatgg gaatattctt ataaaccagt
    68521 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    68581 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagccc gtaagcaccg
    68641 ttcaaccacg cgaaaccact gacgcgtcgg taacgaacac ccgggcggca aaaacaacag
    68701 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    68761 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    68821 taaaaatgga caccggcaaa attgccgatg cgctgctcaa cgaagcgcag caagacttgc
    68881 agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt
    68941 aacgatctca aaacggtaat ggatcaagaa cagcaacatc tctctatggg gcagatcaac
    69001 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac
    69061 ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    69121 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    69181 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    69241 gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg cggtaaaaag
    69301 atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    69361 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    69421 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    69481 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    69541 caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg
    69601 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    69661 cccagcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt
    69721 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    69781 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    69841 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    69901 cgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa gacgcaaccc
    69961 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    70021 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg
    70081 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    70141 gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac
    70201 cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    70261 cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc
    70321 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    70381 gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat
    70441 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    70501 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    70561 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag
    70621 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    70681 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    70741 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    70801 ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    70861 ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg ggcaaaacgc
    70921 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg
    70981 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    71041 gcgccagcac tgcctgttcg ccggcggttt gcagaactgt ctgattttgc acacattcaa
    71101 tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa
    71161 tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt
    71221 cgatgagcgc atcgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    71281 gcatacaggt ggtgatctgc aaaggatcgg ccgaaaaatg gtgctcggca aacagcattt
    71341 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    71401 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    71461 gcccgacact attgctacga tgtttatcca ttcttagcga agctgcggta gcgagttgcg
    71521 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    71581 ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct gccagcggtt
    71641 tatcgacaca gacatgtaca cccgcattga gtaacgtact gaccacgtca aagtggctgg
    71701 cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat aacgaatcgg
    71761 cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta ggcgaccagg
    71821 ctccttgtaa catccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa
    71881 tgccgcctaa tcccacaacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    71941 agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    72001 gcctgtaaat caccatcaac cgcgttcgac atatccgtta ccgccggctg atcctctacc
    72061 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    72121 accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc cacttccgcc
    72181 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    72241 ccacgcaata acaacgtggt gattaacgcc acttccgctg cgctcagttt cagatcgcca
    72301 aattctgaat tacaaaaacg ttgttcatat ttggtgaccc gattaccaaa gccactcacc
    72361 gtgcgcagat aatggcgttt gaccagatta tccagttgct cctgcacttc agattcactc
    72421 agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact
    72481 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aatcacgcga
    72541 gcttcaaggg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    72601 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    72661 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    72721 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    72781 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    72841 accgagatag caggcatgaa aagagccacg aacaacattg gaaaaattgg caacgccaat
    72901 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    72961 ttcgttaatc atccccagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    73021 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg caagacgcca
    73081 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    73141 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    73201 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc
    73261 tgcattattc acatatttga taaaaactct ctatcaaagc gatgttttga taaaaaaata
    73321 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    73381 ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt
    73441 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    73501 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    73561 acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    73621 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    73681 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    73741 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgttg
    73801 tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt
    73861 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt
    73921 tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg
    73981 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    74041 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    74101 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    74161 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    74221 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    74281 cagcggtctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    74341 cgagcctgcg cgtgaaacct taagtgcttt gctggcggac gcaagagctc gcggcagcta
    74401 tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg
    74461 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    74521 aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgcaac
    74581 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    74641 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    74701 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    74761 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    74821 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    74881 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    74941 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    75001 tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    75061 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    75121 tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    75181 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    75241 gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    75301 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    75361 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    75421 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    75481 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    75541 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    75601 gcgtggctga cgcccatatt gttctgacaa acttgatcag ccaaattggt cgcgaagagc
    75661 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    75721 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    75781 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    75841 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    75901 aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg
    75961 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag
    76021 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    76081 ccggcaaaaa cattccttca gccggagcat agagattaat gactgcacca tcccaggtat
    76141 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    76201 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    76261 cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    76321 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    76381 atgagctgga gcgcggattt aatgaaggcg tgttcaccgc tgcaaaactt tacccggcaa
    76441 acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg
    76501 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    76561 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc
    76621 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    76681 tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    76741 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac
    76801 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc
    76861 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg
    76921 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    76981 ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat gggttgccgg
    77041 tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac
    77101 tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    77161 aataaaaatc gtagcttcct gttgtcatta ggttatttaa cctgtataaa taaccagtat
    77221 attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    77281 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    77341 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    77401 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    77461 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg gtcgccccgg
    77521 cttttttatt ttacttacga attttgagta caaaatttct acacttcctc aaaataattg
    77581 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    77641 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    77701 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    77761 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    77821 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    77881 aaataagtcc gagcaggaag ggactgaagt tggtcaaacg ctctggttaa ccactgatgt
    77941 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    78001 ccaggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    78061 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    78121 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    78181 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    78241 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    78301 ttatggtgaa ggcgaaaagt atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga
    78361 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
    78421 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
    78481 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
    78541 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    78601 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
    78661 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
    78721 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    78781 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
    78841 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    78901 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    78961 tgaacgtgtt ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc cgttcttgcg
    79021 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    79081 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
    79141 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    79201 tgcgcaagac aaaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    79261 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    79321 ttgcactatc tca
//
LOCUS       DAJTXQ010000003        74295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000003 DAJTXQ010000000
VERSION     DAJTXQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.003"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..943
                     /locus_tag="OO515_000170"
     CDS             <1..943
                     /locus_tag="OO515_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6574205.1"
                     /translation="PMLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSV
                     VTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            979..1665
                     /gene="mlrA"
                     /locus_tag="OO515_000171"
     CDS             979..1665
                     /gene="mlrA"
                     /locus_tag="OO515_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6574206.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1725..1832
                     /locus_tag="OO515_000172"
     CDS             1725..1832
                     /locus_tag="OO515_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6574207.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1813..2544)
                     /gene="yehW"
                     /locus_tag="OO515_000173"
     CDS             complement(1813..2544)
                     /gene="yehW"
                     /locus_tag="OO515_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6574208.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2549..3475)
                     /gene="yehX"
                     /locus_tag="OO515_000174"
     CDS             complement(2549..3475)
                     /gene="yehX"
                     /locus_tag="OO515_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6574209.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3468..4625)
                     /gene="yehY"
                     /locus_tag="OO515_000175"
     CDS             complement(3468..4625)
                     /gene="yehY"
                     /locus_tag="OO515_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6574210.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4632..5549)
                     /gene="osmF"
                     /locus_tag="OO515_000176"
     CDS             complement(4632..5549)
                     /gene="osmF"
                     /locus_tag="OO515_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6574211.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5801..8098)
                     /gene="bglX"
                     /locus_tag="OO515_000177"
     CDS             complement(5801..8098)
                     /gene="bglX"
                     /locus_tag="OO515_000177"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6574212.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8294..10009
                     /gene="dld"
                     /locus_tag="OO515_000178"
     CDS             8294..10009
                     /gene="dld"
                     /locus_tag="OO515_000178"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6574213.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10048..10980)
                     /gene="pbpG"
                     /locus_tag="OO515_000179"
     CDS             complement(10048..10980)
                     /gene="pbpG"
                     /locus_tag="OO515_000179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6574214.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11154..11741)
                     /locus_tag="OO515_000180"
     CDS             complement(11154..11741)
                     /locus_tag="OO515_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6574215.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11911..12489
                     /locus_tag="OO515_000181"
     CDS             11911..12489
                     /locus_tag="OO515_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6574216.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12619..13380)
                     /locus_tag="OO515_000182"
     CDS             complement(12619..13380)
                     /locus_tag="OO515_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6574217.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13433..14869)
                     /gene="mdtQ"
                     /locus_tag="OO515_000183"
     CDS             complement(13433..14869)
                     /gene="mdtQ"
                     /locus_tag="OO515_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6574218.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15093..15176
                     /gene="yohP"
                     /locus_tag="OO515_000184"
     CDS             15093..15176
                     /gene="yohP"
                     /locus_tag="OO515_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6574219.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15549..16496)
                     /gene="dusC"
                     /locus_tag="OO515_000185"
     CDS             complement(15549..16496)
                     /gene="dusC"
                     /locus_tag="OO515_000185"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6574220.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16735..17133
                     /locus_tag="OO515_000186"
     CDS             16735..17133
                     /locus_tag="OO515_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6574221.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17130..17825
                     /locus_tag="OO515_000187"
     CDS             17130..17825
                     /locus_tag="OO515_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6574222.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17955..18839
                     /gene="cdd"
                     /locus_tag="OO515_000188"
     CDS             17955..18839
                     /gene="cdd"
                     /locus_tag="OO515_000188"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6574223.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18989..19708
                     /gene="sanA"
                     /locus_tag="OO515_000189"
     CDS             18989..19708
                     /gene="sanA"
                     /locus_tag="OO515_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6574224.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19711..19950
                     /locus_tag="OO515_000190"
     CDS             19711..19950
                     /locus_tag="OO515_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6574225.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20144..21382
                     /gene="preT"
                     /locus_tag="OO515_000191"
                     /pseudo
     CDS             20144..21382
                     /gene="preT"
                     /locus_tag="OO515_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21376..22611
                     /gene="preA"
                     /locus_tag="OO515_000192"
     CDS             21376..22611
                     /gene="preA"
                     /locus_tag="OO515_000192"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6574226.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22738..23748)
                     /gene="mglC"
                     /locus_tag="OO515_000193"
     CDS             complement(22738..23748)
                     /gene="mglC"
                     /locus_tag="OO515_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6574227.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23764..25284)
                     /gene="mglA"
                     /locus_tag="OO515_000194"
     CDS             complement(23764..25284)
                     /gene="mglA"
                     /locus_tag="OO515_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6574228.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25345..26343)
                     /gene="mglB"
                     /locus_tag="OO515_000195"
     CDS             complement(25345..26343)
                     /gene="mglB"
                     /locus_tag="OO515_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6574229.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26623..27663)
                     /gene="galS"
                     /locus_tag="OO515_000196"
                     /pseudo
     CDS             complement(26623..27663)
                     /gene="galS"
                     /locus_tag="OO515_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27805..28962)
                     /locus_tag="OO515_000197"
     CDS             complement(27805..28962)
                     /locus_tag="OO515_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6574230.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28979..29647)
                     /gene="folE"
                     /locus_tag="OO515_000198"
     CDS             complement(28979..29647)
                     /gene="folE"
                     /locus_tag="OO515_000198"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6574231.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29905..30741
                     /gene="yeiG"
                     /locus_tag="OO515_000199"
     CDS             29905..30741
                     /gene="yeiG"
                     /locus_tag="OO515_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6574232.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30773..32764)
                     /gene="cirA"
                     /locus_tag="OO515_000200"
     CDS             complement(30773..32764)
                     /gene="cirA"
                     /locus_tag="OO515_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6574233.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33056..34525)
                     /gene="lysP"
                     /locus_tag="OO515_000201"
     CDS             complement(33056..34525)
                     /gene="lysP"
                     /locus_tag="OO515_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6574234.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34730..35611)
                     /locus_tag="OO515_000202"
     CDS             complement(34730..35611)
                     /locus_tag="OO515_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6574235.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGILSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35710..36759
                     /locus_tag="OO515_000203"
     CDS             35710..36759
                     /locus_tag="OO515_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6574236.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36833..37690
                     /gene="nfo"
                     /locus_tag="OO515_000204"
     CDS             36833..37690
                     /gene="nfo"
                     /locus_tag="OO515_000204"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6574237.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37694..38782
                     /locus_tag="OO515_000205"
     CDS             37694..38782
                     /locus_tag="OO515_000205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6574238.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38838..40088)
                     /locus_tag="OO515_000206"
     CDS             complement(38838..40088)
                     /locus_tag="OO515_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6574239.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40188..41129)
                     /gene="rihB"
                     /locus_tag="OO515_000207"
     CDS             complement(40188..41129)
                     /gene="rihB"
                     /locus_tag="OO515_000207"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6574240.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41259..41957
                     /gene="yeiL"
                     /locus_tag="OO515_000208"
     CDS             41259..41957
                     /gene="yeiL"
                     /locus_tag="OO515_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6574241.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42024..43274)
                     /locus_tag="OO515_000209"
     CDS             complement(42024..43274)
                     /locus_tag="OO515_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6574242.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43368..44306)
                     /gene="psuG"
                     /locus_tag="OO515_000210"
     CDS             complement(43368..44306)
                     /gene="psuG"
                     /locus_tag="OO515_000210"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6574243.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44294..45235)
                     /locus_tag="OO515_000211"
     CDS             complement(44294..45235)
                     /locus_tag="OO515_000211"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6574244.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45693..47384)
                     /gene="fruA"
                     /locus_tag="OO515_000212"
     CDS             complement(45693..47384)
                     /gene="fruA"
                     /locus_tag="OO515_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6574245.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47401..48339)
                     /gene="fruK"
                     /locus_tag="OO515_000213"
     CDS             complement(47401..48339)
                     /gene="fruK"
                     /locus_tag="OO515_000213"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6574246.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48339..49469)
                     /gene="fruB"
                     /locus_tag="OO515_000214"
     CDS             complement(48339..49469)
                     /gene="fruB"
                     /locus_tag="OO515_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6574247.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49837..51018
                     /gene="setB"
                     /locus_tag="OO515_000215"
     CDS             49837..51018
                     /gene="setB"
                     /locus_tag="OO515_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6574248.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51015..51269)
                     /locus_tag="OO515_000216"
     CDS             complement(51015..51269)
                     /locus_tag="OO515_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6574249.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51424..51996
                     /gene="yeiP"
                     /locus_tag="OO515_000217"
     CDS             51424..51996
                     /gene="yeiP"
                     /locus_tag="OO515_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6574250.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52219..53685
                     /locus_tag="OO515_000218"
     CDS             52219..53685
                     /locus_tag="OO515_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6574251.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53803..54789
                     /gene="yeiR"
                     /locus_tag="OO515_000219"
     CDS             53803..54789
                     /gene="yeiR"
                     /locus_tag="OO515_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6574252.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54828..55541
                     /gene="lpxT"
                     /locus_tag="OO515_000220"
     CDS             54828..55541
                     /gene="lpxT"
                     /locus_tag="OO515_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6574253.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55953..56519
                     /gene="mepS"
                     /locus_tag="OO515_000221"
     CDS             55953..56519
                     /gene="mepS"
                     /locus_tag="OO515_000221"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6574254.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56700..58256
                     /locus_tag="OO515_000222"
     CDS             56700..58256
                     /locus_tag="OO515_000222"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6574255.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58338..60152
                     /locus_tag="OO515_000223"
     CDS             58338..60152
                     /locus_tag="OO515_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6574256.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60153..61247
                     /locus_tag="OO515_000224"
     CDS             60153..61247
                     /locus_tag="OO515_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6574257.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61247..62272
                     /locus_tag="OO515_000225"
     CDS             61247..62272
                     /locus_tag="OO515_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6574258.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62274..63863
                     /gene="yejF"
                     /locus_tag="OO515_000226"
     CDS             62274..63863
                     /gene="yejF"
                     /locus_tag="OO515_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6574259.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63867..64211)
                     /locus_tag="OO515_000227"
     CDS             complement(63867..64211)
                     /locus_tag="OO515_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574260.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64544..65664)
                     /locus_tag="OO515_000228"
                     /pseudo
     CDS             complement(64544..65664)
                     /locus_tag="OO515_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65692..66387)
                     /gene="rsuA"
                     /locus_tag="OO515_000229"
     CDS             complement(65692..66387)
                     /gene="rsuA"
                     /locus_tag="OO515_000229"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6574261.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66537..68297
                     /locus_tag="OO515_000230"
     CDS             66537..68297
                     /locus_tag="OO515_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6574262.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68422..68706
                     /gene="rplY"
                     /locus_tag="OO515_000231"
     CDS             68422..68706
                     /gene="rplY"
                     /locus_tag="OO515_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6574263.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68845..69852)
                     /gene="yejK"
                     /locus_tag="OO515_000232"
     CDS             complement(68845..69852)
                     /gene="yejK"
                     /locus_tag="OO515_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6574264.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70034..70261
                     /locus_tag="OO515_000233"
     CDS             70034..70261
                     /locus_tag="OO515_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6574265.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70281..72041
                     /gene="pbgA"
                     /locus_tag="OO515_000234"
     CDS             70281..72041
                     /gene="pbgA"
                     /locus_tag="OO515_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6574266.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72116..72192
                     /locus_tag="OO515_000235"
     tRNA            72116..72192
                     /locus_tag="OO515_000235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72150..72152,aa:Pro,seq:ggg)
     gene            complement(72295..74295)
                     /gene="yejO"
                     /locus_tag="OO515_000236"
                     /pseudo
     CDS             complement(72295..74295)
                     /gene="yejO"
                     /locus_tag="OO515_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18241 a        19576 c        18243 g        18235 t
ORIGIN      
        1 gccaatgtta ggtgaaaaaa gactggtttc atctgtaaca aaagaggatt tgcttttcgt
       61 aaggagagat ctgttgaccg gttatcaaaa gctttctaac ggaaaaattt cttccataaa
      121 aggacgctca gtggtcacag taaactacta tatgacaacc atagctggaa tgtttcaatt
      181 tgcaacagat aatggttata catcaggaaa tccatttaac ggtctgacac cattaaaaaa
      241 gtccaagata gaaccagatc ccctcacccg tgacgaattt attcgtttta ttgaggcttg
      301 ccgtcatcaa caaacaaaaa acctgtggat tatcgctgta tacacgggta ttcgtcacgg
      361 ggagctggta tcactggcct gggaagatat agaccttaaa gcgaggacta taaccatccg
      421 ccggaattat acaaaactag gcgaattcac tccaccaaaa actgatgctg gtaccggaag
      481 aacgattcat ctagttcaac cagctattga tgctcttaaa agccaagcgg aaatgaccat
      541 gcttggaaaa cagcattctg tagaggtaaa gcagagggaa tatgggagaa gtactgtgca
      601 taaatgcact tttgttttta gtcctcaggt aataaaacag cggcagtttt ccggaccgca
      661 ctataaggtt gactccatca gggagtcatg gacaagtatc ttaaaacgcg caggtctgag
      721 acacagaaaa tcgtaccaat ccaggcatac ttatgcatgc tggtcacttg ccgctggagc
      781 taatcctagt tttatcgcaa gccagatggg ccacacaaac gcacaaatgg tattcaatgt
      841 ttacggagca tggatgaaag acaacaatca cgaacagata gaactcctta acaaaagact
      901 atctgaaagt gtcccatgta tgccccataa gaaagtgggg taaaataaaa acttgtaaaa
      961 tcagttagtt tacccttaat ccctgtcacg ttacgcgcgt ggcagaggcg ttacgggttg
     1021 ctgaaaccgc aacggacaga cggcggtcat cgactgttca acgatgccga tattgaccgg
     1081 atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg
     1141 ctcagtaatg aaaatgttga tgtgcagaac ggctggcgcg atcagcaaga aacattactg
     1201 acctacctgc aaagcggcaa tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag
     1261 gattaccccg cccagacact caccacacat ctgtttattc ctctgcgccg acggcttcag
     1321 tgccaacaac cgactctcca ggcgctgctg gcgatcctcg acggcgtact gatcaactac
     1381 atcgccattt gtctggcttc ggcacgtaaa aaacagggta aagatgcgct ggtggttggc
     1441 tggaatattc aggataccac ccgtctgtgg ctggagggct ggattgccag tcaacaagga
     1501 tggcgcattg atgtcctcgc ccactcgctc aatcaactac gccctgaact attcgaaggc
     1561 cgtacattgc tggtgtggtg cggtgaaaat cgaacctccg cccaacagca gcaactcacc
     1621 agttggcaag aacaaggcca tgatattttc ccactcggca tttaatgatt cgttaacaaa
     1681 tgcgctttac tgtacaatcc tttcgttaac ataaggagtg caatatgcgc atagctaaaa
     1741 ttggggtcat cgccctgttc ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg
     1801 caggttatac ctttattttg cgtgctggct aagcgcctgc accagccttt caaacaggcg
     1861 gtctgcgatg atcgccgcca gtgccaccag taacgccccc tggatcacat acgcggtatt
     1921 aaatccgcta agcccgatga tgatgggcgt acccagcgtg ctggccccta ccgttgaggc
     1981 gatcgtcgcc gtaccaatgt tgataatcac cgaagttcgc acgcccgcca gaatcaccgg
     2041 agccgccagc ggtagctcga ccttacgcag tcgctgacca cgactcattc ccataccttt
     2101 cgcaacttct gtcacgctgg catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac
     2161 gggcagcaca ccgtaaagga tcaaggcgat aatcgctggt tgcagaccaa agccgatcac
     2221 cggaacggca atcgccagca ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt
     2281 ttccaccagt gggcgaaatt ccgcgcccca cgggcgagtg acagcaattc cggcaccagt
     2341 gccaatgatc accgcaaaca aactcgaaat tcccaccagc cagaaatgag ccagtgccag
     2401 agctgcaaaa ctttcttgct gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa
     2461 cagcggctgg ctgtaaggca gccagaaaat cagtgccaca aacaaagcaa tgagccagaa
     2521 cagcggatcg cgcaacatct tcatacgctt acgcctcctc cagcagatcc tgaaaatgca
     2581 gcgtgccgca aggctggccc tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga
     2641 caaacaggga gagcgcatcg cgtagcgtca tctcttctgc cagtgcctca ccttctgccc
     2701 gttcttcgcg acgcacgtaa tccgccacac tacgtaacga aagcaggcgc acacccagtt
     2761 cgctgcgccc aaaaaactgg cggacaaaat cgtttgccgg acgagtcagc atcgtcagcg
     2821 gattgccctg ctgcaccact tcaccgtgat ccatcaatac cagatgttct gccagccgta
     2881 gcgcctcatc aatatcatga gtgaccagca caatggtacg ccccagcaaa cggtgaatgc
     2941 gcgtcatctc ttgttgcaac gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat
     3001 ccatcagtaa gacttgcgga tcggcagcca gtgcgcgcgc cactcccaca cgttgctgct
     3061 gaccaccgga aagctgatgc ggataacgct cacgtaaatt tgactccagc cccagtagcg
     3121 ccattaattc gtcgatacga tcgtcaatcc tcgcccgcga ccatttttgt aattgcggca
     3181 cggtagcgat gttttgcgcc acgctccagt gggggaacag gccaatagat tgaatggcat
     3241 agcccatccg gcggcgcaac tccagtactg gcagcgagcg aatttcttct ccggcaaagc
     3301 ggatcactcc gctgtcatgc tccaccaggc ggttaatcat tttcagggtg gtggatttgc
     3361 cggagccaga tgtgccaatc agcaccgaaa aactcccttc ctgaaaattg agattgagat
     3421 cgttaacggc tttttgtgcg ccgaacagtt tgctgacatg gccaaattca atcattacgt
     3481 ttcaccttca gcagtgcgat aagcaaatcg aacagcgcgt cggtaagcac cgccagaaca
     3541 attaccggga tcacccccag caacactaaa tcaatggcgc tgcttagcag cccctggaaa
     3601 accagcgcac caaaaccgcc tgcgccgatt aacgccgcaa tcaccgccat acctacagtt
     3661 tgcaccatca ccacccgcag gctgcgcaga aataccggta acgccagtgg taactgaaca
     3721 tgcaggaatc gctgcgcccc gctcatgccc atcgctctgg cgctctccag cacatcgcgc
     3781 gggatctggt tcaaaccgac taccacgccg cgcaccagcg gcagcaaggc atagagcacc
     3841 agcgcaatca gtgcgggtgt cattccggtt cctgctatgc cgagcgtccc cagccacgga
     3901 aaggccgtaa ccagcgcggc aagcggcgca atcaacaggc caaagagcgc caccgaaggc
     3961 acggtctgaa tgacattgag cagagaaaaa attgccccct gccgagcagt ggaaaagtag
     4021 caccagatgc ccaacggcac accaatcact aacgcaggca gcaccgcacc aaacagcaac
     4081 gtcagatgtt gtgccagcgc gtcgtcaaac acatcctgac ggttggcgta ttctttcatt
     4141 agtgagagat cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc
     4201 atatgcaaca accagcgcca cagcggatgc gtggagattc ggcggatggc atcactacag
     4261 gccagcaatg ccagcgccgc ggccagccag aaaccactgc cgaggctggt acgcgccagc
     4321 gcactgccat tttgcgccag ttgggtcgcc gcctttcctg ctccccacac cagcaatacg
     4381 aagacgaatt gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg aataaaacag
     4441 gctgtcagcc aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc
     4501 cagagatgac gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc
     4561 gccgctgcga ttgccgtcag caacagcagc agcgccagaa caggattaat acgtagataa
     4621 gtcacgggaa attacttcgt ccaccctttt tgtttcaggt agtcggcagc cacttttttg
     4681 gcatccagtc cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg
     4741 ctggcgaaga ctggctgtag ccactgtgcc atttgcggat actctttcag caccgactca
     4801 cgcaccactg gtgcaggcgc gtagataggt tgcacgcctt tcggatcgct taaggtttgc
     4861 agccccagcg ccgcgaccgg accgtcagtg ccgtaagcca ttgcggcatt aacgccagaa
     4921 gtttgctggg cagcggcttt aatcgttacc gccgtgtcgc cgccagccag tgacagtaac
     4981 tgatcctgat cgagtttaaa gtcgtaggct ttttcaaacg cgggtaacgc atcggcgcgt
     5041 tcgataaact ctgccgaggc tgccagtttg aaggtgccgc cctctttcag ataacgactc
     5101 aggtcggcaa gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatggtc
     5161 caggtgttat tcgctggtgc gggcgtcagc cagattaact tgttttgctc tgcatcgagt
     5221 tttttgactt tctcgtaacc ttgctgcgcg tttttccatg ccgcatcgtt ttcatcttta
     5281 aagaagaaag cgccattacc ggtatattcc ggatagatat ccagttcacc ggaagtaatc
     5341 gccccgcgca ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg
     5401 ctttccagca cctgcaagat gatgttgccg agtagcgcgc cttcggtatc gatttttgaa
     5461 ccgactttaa cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt
     5521 gaacctgccc agacctttga gagtggcatg atgctttcct cattctttta ctgttgtttt
     5581 cagcgaatta agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc
     5641 agttgcagaa tcagataaat acttaaaggg catattttgc ccggcgtttc ttgccggatg
     5701 cggcgtaaac gccttatccg gcctacaaag gtcgaaaatt cagcaagtta agatatgaac
     5761 atcaggcaaa gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc
     5821 ctttcttaac gcgtgcggaa tcagtgccga taaagacatt gaacttgcca ggttcggcgt
     5881 catatttcat ctgttgattc cagaacttca gcgcctcgat atcaatcggg aagctgacag
     5941 tctgagtttc gcccggtttc agggtgattt tctcaaagcc tttcagctgc ttcactggac
     6001 ggctcatgga agccgtcaca tcctgcaagt acatctgtac taccgtggcc ccttcgcgtt
     6061 taccggtgtt cgtcacctgc acgctggcgg tcactttgcc gtcacgcttc atggtcggcg
     6121 cagaaagttt cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat
     6181 aaagcgcccc gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat
     6241 tatacggacg accggtattc agatggctgt agtacaccgg gatctgcccg acagaacgcg
     6301 ggaaggacat tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg
     6361 cattaccgcc ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt
     6421 ctttcaccag cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg
     6481 ctttcagcgc cgtaatcaag tcacgttggc tttgcggaat agtgatatcg gtacggctgg
     6541 aggcctcgtg cgccatcccc tgtgcttcac cgaccacagc caccaccaca tcagattgtt
     6601 tcgccgtctg caccgcttca tcaatcatct cttgcggcga gcgcggatcg actttgaccg
     6661 cttcttcata ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt
     6721 tggcatacag cactttaccg ttttcaccga cggcattttt aatcccggtc agtacggtca
     6781 cggattgatc ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg
     6841 ccagtggccc aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt
     6901 ttttcagcaa caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc
     6961 tttcggcatt ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt
     7021 taaacaatcc catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctt
     7081 ccatcgtcac tttgccggat ttgatcaacc caggcagata cttcgagtaa tactcgtcgc
     7141 tcatactcat gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg
     7201 ccgtgccatg tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa
     7261 agccccactg gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc
     7321 cgttcagcga gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt
     7381 acggcggcat ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt
     7441 taccgccttc taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtagc
     7501 gatctgccgg gcttttaccc tgcatcgctt ccaccatggt tttacccatt gttgaggtga
     7561 gatacgtatc ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat
     7621 cgaccatcgg tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac
     7681 gtccgaccgt tttcactgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga
     7741 acaccgtgcg ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc
     7801 ttaattccat cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa
     7861 tcgccccaac ctgaccgtct ttgatcatct cgcggatcgc ctctttcggg ttatccggac
     7921 cgacgctgat taagcgcagc tgaccaattt tctcatcaac tgtcattttc ttaagcagtt
     7981 cggtgacgaa cgcatcccgc gcttcgggcg ttaatggatg gttgccgaat aaatcatccg
     8041 ccagtgctgg ctgcagggcc agactcaccg cgattcctac tgaacatagc catttcatat
     8101 ggatttttct tcctctgttg cccgacgagg cagcgaaaat ggtgcaaaaa ccgtagtttg
     8161 ccataagcat gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata
     8221 ttcactcgct gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca
     8281 caaggagtgg aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact
     8341 tgcccgtctg gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg
     8401 caagggcttc cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact
     8461 ggaattgtgg cgggtgctga aagcctgtgt caccgccgac aaaattattc tgatgcaggc
     8521 tgccaataca ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat
     8581 cgtgatcatc agcaccctgc gtctcgacaa gctgcacgtt cttggcaagg gcgaacaggt
     8641 gctggcctat ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg
     8701 cgaaccgcac tcagtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg
     8761 taacaactcc ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt
     8821 cgcgcgtata aatgaagacg gcaaactgac gctggtgaac catctgggga ttgatctggg
     8881 cgaaacgccg gagcagatcc ttagcaagct ggatgatgat cgcatcaaag atgacgatgt
     8941 gcgtcacgat ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga
     9001 agccgacacg cccgcacgtt ataacgccga tcctgatcgg ttatttgaat cttctggttg
     9061 cgccgggaag ctggcggtct ttgctgtacg tctagatacc ttcgaagcgg aaaaaaatca
     9121 gcaggtgttt tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat
     9181 tctggctaac ttcgaaaatc tgccggttgc cggggaatat atacaccggg atatctacga
     9241 tattgcggaa aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa
     9301 gatgccgttc ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt
     9361 cttccgtccg cattttactg accgtgcgat gcaaaaattc ggtcacctgt tccccagcca
     9421 tttaccgccg cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat
     9481 ggcgggcgat ggcgtgggcg aagccaaatc gtggctggtg gattatttca aacaggccga
     9541 aggcgatttc tttgtctgta cgccggagga aggcagcaaa gcgtttttac accgtttcgc
     9601 cgctgcgggc gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct
     9661 ggcgttggat atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga
     9721 gatcgacagc cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca
     9781 tcaggattac atagtgaaaa aaggcgtgga tgtgcatgcg ttgaaagaac agatgctgga
     9841 actgctacag cagcgtggcg cgcaataccc tgccgagcat aacgtcggtc atttgtataa
     9901 agcaccggag acgttgcaga agttttatcg cgagaacgat ccgaccaaca gtatgaatcc
     9961 ggggatcggt aaaaccagta agcggaaaaa ctggcaggaa gtggagtaaa aattacggat
    10021 ggcatagtat cgccatccgt cattcactta atcgttctgt gccgtctgcc ccgccgccgc
    10081 catttgggcg gctttttgtt ttttatagct caacgctgct gctggaacag gcatcacttt
    10141 accagtttca atccaggtac gcaggcggct ggcatcggca aaatgggtat atttgccaaa
    10201 cgcatccatc actaccagcg ctaccggttt attattgata accgtacgca tcaccagaca
    10261 atggcccgcc gcattggtaa aaccggtttt ggttaactga atattccagt tatcgcgata
    10321 caccagatgg ttagtattgc ggaacggcag cgtatacgtc gggttagaga aggttgccat
    10381 atcttcccgg gtagtactta actgcccgat caacggatat tgtttgctgg caatgagcag
    10441 tttggttaag tcacgggcag ttgaaacgtt atgcaccgac aatccggtag gttcaacaaa
    10501 gcgcgtgttg ttcattccga gcgatttcgc tttcgcattc attgccttaa taaaggcttt
    10561 gtaaccaccg ggataatggt gtgcaaggct tgccgccgcg cggttttctg aagacatcag
    10621 cgccagcaac agcatatctt tacggctgat ttcgctattc agtcgtacgc gcgaatagac
    10681 ccctttcatc tccggcgtct ggctgatatc cacttttagt ttttcatcca gcggcagtcg
    10741 tgcatccagc acaaccatcg cggtcattaa tttgctgata gacgcaatcg gacgcaccag
    10801 atccgggtgg ttcgagtaga tcactttgtt ggtattcaga tcaacaatca tcgcgctacc
    10861 ggaggcaatt tcaggttgtg aagcggtggt agcggctgcc gttttcgcaa cggcctgcgg
    10921 tgccaaaggc acagccagca tcagggccag gctaaataaa gaaactcgaa atttcggcat
    10981 gatgagcatt cagatagtgg ttcacgcgca caggttgcgc accgccggag taaggattta
    11041 ctgaggctag cgacgccatc ataacgagca aaaagtgcga tcgtcaaagg agaatcgtga
    11101 ggaaatgctg cattgctgac atttacgcca gcaatgcaac gtcaaagaac tttctagaac
    11161 aaacgataac cgtagcccca tagtataacg gttagggcga gcagcacttc cagtaccagc
    11221 acgccaatcg ccagcgtcga actagagaag ctaaggcctt cctctttgtt gatattcagg
    11281 aagctcggaa taccaaggta aagcaggtag ccggtgtaaa acagcgccac cgtgccgacc
    11341 agcgcacaca accagaccag tggataaagc gccaccagac cgcttaaaaa cagcggagtt
    11401 gcaacgtagc ccgcgaagac catgcagtgc gcaagtgacg gacgctgcgg ataattacgc
    11461 gccatccacc agatgacccg ccccattacc gcaaccccag ccagcataac gccataaaac
    11521 aggacagcca gcgccagtcc ggtaaaccag gataacttca ggatagtgcc atcgccaaaa
    11581 ttccagccaa tctgtgtagt gccaatgaag gcgcaaatca ccggaatcgc cgccatcagc
    11641 aaaacgtggt gggtgtaatg gtgagaaatc gtttcgtttt cgcgattaat cacctgcatt
    11701 tcacgatcgg gatgggaaaa cagtccccag acatggctca taccgccccc ttgttgtgag
    11761 ttcatgaacc tgacagttca agtataagtc agcttgtgat tattttttgt tcgctcatgt
    11821 aatttgcacc gtctgatgta cgtccttttt cagcgtgaat gatttccagg gtgtatgctt
    11881 acaggcaacc aaagggagac agactggcct atggatctca atacactcat ctcacaatat
    11941 ggttatgccg cgctggtgat cggtagcctg gcggaaggtg aaaccgtgac tttgctggga
    12001 ggcgttgcgg cacatcaggg gctattaaag ttcccgctgg tggtactttc tgtggcgctt
    12061 ggcggcatga ttggcgacca ggtgctttat ctgtgcgggc ggcggtttgg cggcaagctg
    12121 ttacgccgtt tctcgaaaca tcaggataaa attgagcggg cgcagaaact tatccaacgc
    12181 catccgtatc tgtttgtcat tggtacgcgc tttatgtatg gctttcgggt gattggcccg
    12241 acgctgattg gtgccagcca gctgccgccg aaaatctttc tgccgctgaa tattctcggc
    12301 gcatttgcct gggcgttgat ttttaccact attggttacg ctggtggtca ggtgattgcg
    12361 ccgtggttgc acaatctcga ccagcatttg aagcactggg tctggttgat tctggtcgtg
    12421 gttctggtgg tgggcgtgcg ctggtggctg aaacggcgcg ggaagaaaaa gccggatcat
    12481 caggcgtaaa accattgccc tgggtaaggc gttcgcgccg catccgacat ccggtgcacc
    12541 atgcctgatg cgacgcttgt gcgtcttatc atgcctacca aaattttacc cattgaaata
    12601 accacgcacc tcactgcgct attctgggtt gaactgcgga ttcgccaaca taaagccgcc
    12661 atccactatc aacgactgcc cggtggtgta atttgcgccc tctgagcaca gccacgctac
    12721 caggctggca atctcatgcg ttgcgccaaa acgccgcaga ggaatcgaag gctccgcgtc
    12781 gggcttcacg tcgctgtcat ccatgccatt cattggcgtg gcgatcgccc caggcgcgac
    12841 tgcgttcacc aaaatcttat gcctgaccag ctccagcgcc atcgttttgg ttaacccgcc
    12901 gagcgcatgt ttagcggctg tgtaagcgct ggcatccggc agcggcgtat gttcatgtac
    12961 cgacgtaatg ttgatgatac gaccgccctg cccttgtttc accatctggc gggccgcaat
    13021 ttgtgagcat aaaaatgcac catcgacatc aacggtaaaa atcttgcgcc actcatcgaa
    13081 agccatatca agaaacggcg ctttggtcat tgcgcccgca ttattcacca gcacatcaat
    13141 acgccccagc cgttgaatga gtttctccag cgcctgcgca ccttctggca gtttaccgag
    13201 ctccagctgc acgatctccg cacgtacgcc gtggctaact acctcacgcg cggtatcttt
    13261 tgccccttct tcatctgagt gccaggtaat gccaatatca aacccctgct gcgccagtaa
    13321 taacgcgcac tctttgccga tccccgaatc ggaggcggta ataatcgcaa cctgtgccat
    13381 caagttctcc acttaacgct gaataaacgt taagtataga aggcgcatat catcagcgtt
    13441 tgtaaccccc gcccaacgca ccagtgagtt gaatagaagc atccagccac tgcccttgca
    13501 ataacaggcc attggcccgc tcacgcagcg cggggattct ggcttcgctg acgcgggaac
    13561 caacaatgat gcccgcgtta aagcgcgcct gcgcaagacc taccacacgc aaggcatcgc
    13621 gctcaatttg cgcctgatgc tggtttttct ccgcaagtgt ctgaacctga ctggctgccc
    13681 gcgccacgtc attcaccgct tcaaccaccg ctttgttgta gctggcgata gacaagttgc
    13741 tttcggcttt tgcgatattg agattggcgt taagacgacc actatcgaaa atgggtagcg
    13801 tcaggcctgc cgtaacgccc atttgctgcg cggaatgacg gaacagatcg cttaagtgca
    13861 acgcatcctg ttgcaggaag gccatcaggt tgatgtcagg ataaaatgcc gcttttgccg
    13921 catcaatggt gcttagcgat gactcaacgt accagtgcgc cgcctgcaaa tctgcccgcc
    13981 gggccagtaa ggagtacccc agttcatcag gaagctggct tgccactttc ggcaacgcga
    14041 ccggtttaag cttcaatgac tttgtctggt tatttgtaag tgcgcttaac cgtgcctcaa
    14101 taattttcat tttccccgcg acatcgttga gctgctgccg ggttttgctg gcattaatat
    14161 cggtttccac accttcaact gaagaagtaa tcccgttctg atatagctgg cgatcggtcg
    14221 cgataatggt gttctgctct ttttctattt gctgcaagac cgtgtttaac gccgcctggg
    14281 tttgccactc ccagtacagg cgggctacgc tgccagccag caattggcgg gtttgctcgc
    14341 gttccgccgc ccgtgcttta accgtaccca ggcgggcagt aacctccgcc cgattctttc
    14401 cccagatatc gagatgccag cccgccgtta agccaaaagt accgttggtg taccacgggc
    14461 cggtcgtacc tgcggccgga tcgttcagag caaacggccc cattaagcct tctgccgaca
    14521 ttttttgccg ctccatatcc gccgaaaagt cgatctgcgg accatcctga gtggcaactg
    14581 ccttcgcctg ggcttcagct agctgaatgc gctgttcagc cacctgcata tccggtgcgt
    14641 tctgtagtgc attgttaatt aatgaagtga gctgattatc gtgatactcc agccaccatt
    14701 ggctgtctgg ccaaccattt ttcagcgccg tgggtaatgc ggtgtcaact tgtgcagcgg
    14761 gcgtttgctg gcttaacgcc tggcgggttt catgcatagg cgcacacccg gccagcatca
    14821 gtaacagcgg aaaacaggcg atggctggat aaaaggaatc acgattcatg ggggaataat
    14881 caggtaagaa acggtgcgcg gagattaccg tgtgttgcga tatatttttt agtttcgcgt
    14941 ggcaatacat cagtagcaat aaaacgacat acccagaaaa atatagacta agtaaatgat
    15001 atcttccgat ttatcttaat cgtttatgga taacggcaaa gggcttcgtt ttttcctata
    15061 cttattcagc actcacaaat aaaggaacgc caatgaaaat tatactctgg gctgtattga
    15121 ttattttcct gattgggcta ctggtggtga ctggcgtatt taagatgata ttttaaaatt
    15181 aattaatgtc atcaggtccg aaaataacga gaatatttca gtctctcatc ctgttgcgct
    15241 cctgtcatgt gcattgcttc atataatcac tggcgcaagg agcgcgcagg gggcggccaa
    15301 tcgccgccgc cccctgcacc cccgggctct ggcgaacaaa atcgccgctg cgcggtgccc
    15361 tcggcttatc ccttacggct accgggtcgg gcgcgaggta acatccctgt aaaacgcgcc
    15421 ctcagcccac ctccatgtgg gctgccccgg ccttcaggga acgcctcggc aattttgacg
    15481 ccaccaaaca accgtgcggc ctattgataa agagctaaca cattgtcaaa aaacatcact
    15541 atggtttttt agagtttctc gatatcaatt gcctgaatag cccttgcaat atcaggggaa
    15601 ttattcaaca cccgaacatg ctgaaataat tccgttgctt catcgtattc tttacgcaaa
    15661 taactcaacc actgtttaat ccgcgcaacg tgatataacc cggtatcgcc ctgcttttcc
    15721 agacgggtat atttttgcag caaagcaacc acctccggcc acggcattcg cggttcgtta
    15781 tattttacta cccggctcag gttgggaata ttgagcgccc cgcgaccaat catcactgcg
    15841 tcgcagccgc tgatcgcaat gcattgttgc gcgctctgcc agtcccagat ttcgccgttg
    15901 gcaatcaccg gaatattcag ccgctggcga atctcgccaa tcgcctgcca gtcaatatgc
    15961 tccgcgcggt aaccctgctc ttttgtccgt ccatgcacca ccagctccgt agcaccagcc
    16021 tgttgaaccg catcggcgat ttcaaatttc ttctcaccgc tgtcccagcc cagacgcact
    16081 ttcacgctga cgggcaaatg cgccggtaca gcttcacgca tcgcttttgc accctggtag
    16141 atgagttcag gatctttgag taacgtcgcc ccgccgccgc tgccgttaac cgttttcgac
    16201 gggcagccgc aattgagatc cacgccccag gaacctaatt ccaccgcacg ggcggcgttc
    16261 tctgccagcc attgtgggaa ctgacctaac aactgaacgc gcaccagcgt accggatggt
    16321 gtccggctgg cgttttgtag ctcagggcaa atgcgatgaa agacttttac cggcagcagt
    16381 tgatccacca cgcggacaaa ctcggtgatg cacagatcgt agtcgttaac ttcggtcagc
    16441 aattctcgca ccagagagtc aagcacaccc tccatcggag ccagtaacac acgcatatct
    16501 tcacccggaa aaaaatgagg cgctatgtta gcgcctatgg tcaacggatt aaaggtctgc
    16561 aaagagggat tgctgcaaac tgtaatccct ctctatgctt gatgtatgcc tgagttcgtt
    16621 gcgcattgcg taatgccggg gagcgaaaaa ataagcgctg atttcttaat gtgatcggta
    16681 gcacgtttta cgaattaatt gtatgatgaa tccatctcat ctggggtgtt gattatgagt
    16741 aagacactga acattatctg gcaatattta cgcgctttcg tcctgattta tgcctgcctg
    16801 tatgcaggca ttttcattgc ttccctgcta ccggtaacca ttccgggcag catcatcggg
    16861 atgctgatcc tgtttgtcct gctggccttg caaattcttc cggcaaaatg ggtcaatccg
    16921 gggtgctacg tactgattcg ctatatggcg ctattgtttg tgccgattgg cgtaggcgtc
    16981 atgcaatatt ttgatttgct ccgcgcacag tttggcccgg tagtggtttc ctgtgcagtc
    17041 agtacgctgg tggtttttct ggtggtgagc tggagttcac aactggtaca cggtgaacgt
    17101 aaagtcgtag gtcagaaagg atcagaagaa tgatggcgaa tatctggtgg tcattaccgc
    17161 tgacgttgat tgtctttttt gccgcccgca aactggcggc acggtataag tttcccctgc
    17221 ttaacccgtt gctggtagca atggtggtga tcattccttt tttaatgctg accggcatct
    17281 cttacgacag ctactttaaa ggtagcgaag tgctaaacga tctgctgcaa ccggcggtcg
    17341 tcgcgctggc ctatccttta tatgagcagc tacaccagat ccgcgcgcgc tggaaatcga
    17401 tcatcaccat ctgttttatc ggcagcgtgg ttgcgatggt gacggggact tccgtggcat
    17461 tgttgatggg cgcttcaccg gaaatcgccg cgtcaatcct gccgaaatca gtcaccacgc
    17521 ctattgcaat ggcggttggc ggcagtattg gcggtattcc ggcaatcagc gcagtttgcg
    17581 tgattttcgt cggcatcctc ggcgcggtat ttggccatac attgcttaat gcgatgcgta
    17641 ttcgtaccaa agctgcgcgc ggtctggcaa tggggacagc ctcgcacgcc ctcggtacgg
    17701 cgcgctgcgc cgagctggat tatcaggaag gtgcatttag ttcgctggcg ctggtgttat
    17761 gcgggataat tacttcgctg atcgcaccgt tccttttccc gattattctg gcagtaatgg
    17821 gctaaaattt gcgatgcgtc gcgcattttt gatgtaagtt tcacgcgttg cataattaat
    17881 gagattcaga tcacatataa agccataacg ggttcgtaaa ctgttatccc attacatgat
    17941 tatgaggcaa cgccatgcat ccacgttttc aaaccgcttt tgcccaactt gcggataact
    18001 tgcaatctgc actggaacct attctggcag acaagtactt ccccgctttg ttgaccgggg
    18061 agcaagtctc atcgctgaag agcgcaacgg ggctggacga agacgcgctg gcattcgcac
    18121 tacttccact ggcggcggcc tgtgcgcgta cgccattgtc gaattttaat gttggcgcaa
    18181 ttgcgcgcgg tgtgagcgga acctggtatt tcggtgccaa tatggaattt attggtgcaa
    18241 caatgcagca aaccgttcat gccgaacaaa gcgcgatcag ccacgcctgg ttgagtggtg
    18301 aaaaagcgct tgcagccatc accgttaact atacgccttg tggtcactgc cgtcagttta
    18361 tgaatgaact gaacagcggt ctggatctgc gtattcatct gccgggccgc gaggcacacg
    18421 cgctgcgtga ctatctgccg gatgcctttg ggccgaaaga tctggagatt aaaacgctgc
    18481 tgatggacga acaggatcac ggctatgcgc tgacgggtga tgcgctttct caggcagcga
    18541 ttgcggcggc aaaccgttcg cacatgcctt acagtaagtc gccaagcggt gtcgcgctgg
    18601 aatgtaaaga cggtcgtatt ttcagcggca gctacgctga aaacgccgca ttcaacccga
    18661 ctctgccacc gttgcagggg gcgttaattc tgttgaatct caagggttat gattacccgg
    18721 atatccagcg cgcggttctg gcagaaaaag ccgatgcgcc gttgattcag tgggatgcca
    18781 cctccgcaac gctgaaagct ctcggctgtc acagtatcga ccgcgtgctt ctcgcttaag
    18841 cctggtgccg gatgcggcgt gaacgccttg tccggcttgc cagccctctc ctggtgtcga
    18901 aattcccggc aaacagtttg ccgtttcttg cgcaaaacca gcgggtaaag tagcctgatg
    18961 gaaattttcc ttagatcgag tctcctgcat gttaaagcgc gtgttcctca gcctgttagt
    19021 cctgatcggc ttgctgctgt tgactgtgct cggcctcgat cgctggatga gctggaaaac
    19081 cgcgccttat atctacgacg aattgcagga tctcccctac cgccaggtcg gcgtggtgct
    19141 cggcacagca aaatattatc gtactggcgt aattaatcag tattatcgct accgcattca
    19201 aggagcgatt aatgcctata acagcggtaa ggtaaattat ctattactga gcggcgataa
    19261 cgcattgcaa agttataatg agccgatgac catgcgcaaa gatttaatcg ctgctggtgt
    19321 cgacccatca gatattgttc tcgattacgc aggctttcgt acgctggatt ccatcgtgcg
    19381 tacacgcaaa gtgttcgata ctaatgattt cattattatc acccaacgtt tccactgtga
    19441 gcgggcatta tttattgcgc tgcatatggg gattcaggct cagtgttatg ccgtaccgtc
    19501 accgaaagat atgctgtcag tacgtattcg cgaatttgcc gcccgtttcg gtgcgctggc
    19561 tgacctttat atttttagac gtgaaccgcg ttttttaggg ccgctggtcc ctattccggc
    19621 tatgcaccag gtgccggaag atgcgcaggg gtatcccgcc gtcacacccg aacagttact
    19681 tgaattacaa aagaaacaag gaaagtagtt atggatgtac agcagttttt tgtcgttgcc
    19741 gtttttttcc ttatcccgat attttgtttc cgcgaagcat ggaaaggctg gcgcgcaggc
    19801 gcgattgata aacgggttaa aaatgcaccg gaaccggtgt atgtctggcg agcaaaaaat
    19861 cccggactct ttttcgctta tatggtggca tatatcggct tcggaatttt atctatcggc
    19921 atgattgttt atcttatttt ctatcgttaa tattttctct tttctgttaa ttattgagaa
    19981 taattattac ttcactcaat aagctgcgga tataacattc tgaaccgcag ctatttgtga
    20041 atcctttcac agtttaaatt tccccacacg cttagcctta atatcagcac gcaatattta
    20101 tttagcgttc gccttaatta ctcatagcat taaggaagat cacatgccgc aacaaaatta
    20161 tctggatgaa ctcactccgg cttttacgcc tttactggcg attaaagaag cctctcgctg
    20221 tttattatgt catgacgctc cctgtagtca ggcttgcccg gcacagaccg atccggggaa
    20281 atttattcgc tcaatctact ttcgtaattt taaaggcgct gccgagacaa ttcgcgaaaa
    20341 taatgccctc ggtgccgttt gtgccagagt gtgcccgacg gagaaattat gccaaagcgg
    20401 ttgtacccgt gccggtgttg atgctcccat tgatatcggc cgcttacagc gttttgttac
    20461 tgattttgaa caacaaaccg ggatggaaat ttatcagccc ggtactaaaa cgctcggcaa
    20521 agtcgcgatt attggcgcag gtcctgccgg attgcaggcc agtgtgacac tgacaaacca
    20581 gggttatgac gtcacgatct atgagaaaga agcacaaccc ggtggctgat tgcgtaacgg
    20641 cattccgcaa ttccggttac cgcaatcagt actggatgca gagatcgccc gtattgaaaa
    20701 aatgggcgtg accattaagt gcaacaacga agtcggtaac acactcaccc ttgagcagct
    20761 gaaagcagaa aaccgcgcgg tactggtcac cgtggggtta tcaagcggtt ccgggctacc
    20821 gctgtttgag catagtgacg ttgagattgc cgtcgacttc ttgcaacgtg cacgacaggc
    20881 gcaaggcgat atcagcattc cacaaagcgc attaattatc ggcggcggtg atgtcgcgat
    20941 ggacgtagcc agcacgctga aagttctcgg ctgtcaggcg gtaacttgcg tagcgcgtga
    21001 agagttagat gagtttccgg caagcgaaaa agagtttacc agcgcccggg agctgggcgt
    21061 ttcgatcatc gatggattca cgccagtagc cgtggaaggc aataaagtca cgtttaagca
    21121 tgtacggtta tcgggcgaac tgacgatggc ggcagataaa attattctcg ccgtcggtca
    21181 gcatgccaga ctagatgcct ttgcggagtt agagccgcag cgtaacacca tcaaaacaca
    21241 aaattaccag acccgcgacc cgcaagtctt tgctgctggc gatattgttg agggagacaa
    21301 aaccgtggtc tatgccgtga aaaccgggaa agaagccgcc gaggcgattc atcactattt
    21361 agagggagct tgctcatgtt aacgaaagat ctttcgatta ctttttgcgg cgtgaagttt
    21421 cccaacccgt tctgcctctc ttcttcgccg gtaggcaact gctatgagat gtgtgccaaa
    21481 gcctacgaca caggttgggg cggtgtggtg tttaaaacga tcggcttttt tatcgccaac
    21541 gaagtctcgc cgcgttttga tcatctggtg aaagaagata ccggttttat cggcttcaaa
    21601 aatatggagc agattgccga gcatccgctg gaggagaatc tggccgccct gcgtcggctg
    21661 aaagaagatt acccggacaa agtattgatc gcttcgatca tgggggaaaa tgagcagcaa
    21721 tgggaggagc tggcgcgcct ggtgcaagaa gctggcgcgg atatgatcga gtgtaacttc
    21781 tcctgtccgc aaatgacttc tcatgcgatg ggtagcgatg tcgggcaaag cccggagctg
    21841 gtagaaaaat attgtcgggc agtgaaacgg ggttccacgc tgccaatgct ggcgaagatg
    21901 acgccgaata tcggtgatat gtgcgaagtg gcgctggcgg cgaagcgcgg cggcgcagat
    21961 ggcattgcgg cgattaacac cgttaaatcc atcaccaata tcgatcttaa tcagaaaatc
    22021 ggtatgccga tcgttaacgg aaaatcaagt atttccggat attccggtaa agcggtaaaa
    22081 ccgatcgccc tgcgcttcat tcagcaaatg cgcacccatc cagaactgcg cgatttccca
    22141 atcagcggta tcggcggcat tgaaacctgg gaggatgcgg cggagttttt attgctcggc
    22201 gcagcaacgt tacaggtcac caccggcatt atgcagtacg gctatcgcat cgtagaagat
    22261 atggcgagcg ggttatcgca ttatctcgcc gatcagggat ttgattcgtt gcaggagatg
    22321 gtaggtctgg cgaataacaa tattgtcccg gcggaagatt tagaccgcag ttatattgtc
    22381 tatccccgta tcaatcttga caaatgtgtt ggctgtggac gctgttatat ttcctgttac
    22441 gacggcggtc accaggcgat ggaatggagc gagaaaaccc gcacaccgca ttgtaatacc
    22501 gagaaatgtg tgggttgtct gctttgtggt cacgtctgcc cggtgggttg tattgagctc
    22561 ggggaagtga agtttaagaa aggcgagaaa gaacacccgg taacgttgta gaacccgcgt
    22621 cacatccggc atttggttcc tgttgccgga tgcggcgtga acgccttatc cggcctacaa
    22681 aaccacacag atcatgatgt tatgtgcgtt tatgaccgaa tgcggaccac attcacatca
    22741 tttcttacgc gcgtatttca gtgaatccag cgctacggcg aagataataa tcgccccttt
    22801 gatgatgtac tgccagtatg ggtttacgcc gatatacgtc aggccatagt tgatgacggt
    22861 aaaaataatc accccggtca ccacgccaat caccgtcccc acaccgccgc tgaacgatac
    22921 accgcctacc acgcacgccg cgatagcatc cagctcatac ataaagccga ggttgttggt
    22981 ggcagagccg atacgtccgg cttctaacat cccgccaaag gcatagaaca cgccagacaa
    23041 cgcgtagatc atcagcaggt tcaggccgac gttgacacca gatacttttg ccgcttccgg
    23101 gttaccgcca atggcaaaaa tgttcttacc gaagcgggtt ttgttccaca acacccagac
    23161 gaacgccacc gcaatcaacg cgtagaaggt gatgtaagag agacggaaac tccccagcgc
    23221 gacaaagccc tgagcaaagg tagagaagcc actgtcaaaa ccagaaattg gcgacgcccc
    23281 gacaaagtcg taatagagcg agttgatgcc atagacgatg atcatcgtgc cgagcgtggt
    23341 aatgaacggc gtcacgttga gataagcgat aatcagaccg ttgatcaaac cgatcaccgc
    23401 accaatggca cagacaatca gaataaccag cgcaatcggc atcgtcgcca tttccgggaa
    23461 cactttgttg gcgttatcca tggactgcaa taatgtcgca gccaccactg ccgccagccc
    23521 tacctgacga ccagcagaaa gatcggtccc ctgggtgaca attaaccctg ccacaccgag
    23581 cgcgataata atacgcaccg atgactgggt gagaatatta cttaagttca acagacttaa
    23641 aaatgttggg tcctggaaaa taataatcgc cagcaaaact aaaagaacga cgtaaatacc
    23701 gccctctttc aggtaagtaa gaaaactttt cttatttaac gcactcatgg gaagccccta
    23761 atcttaaagg tgcaaagacg caagacgcag aatttcgttt tgcgttgttg tttttgtatc
    23821 gacaattccg gaaacgagac cattgctcat gaccagaata cggtctgtta tccctaacaa
    23881 ctcaggcatt tcagaggaga taataataat ccccttgcct ttcttcgcca gttcggcaat
    23941 taactgataa atttcaaact tcgccccgac atcaataccg cgcgtcggtt catcgagcat
    24001 taatatttct ggttgcgtta gtagccagcg accaataatc accttttgct gattaccacc
    24061 ggagagcgaa ccaatttgcg tccgatgacc cggcgttttt acccgcatcg aatcaatcac
    24121 ccactgggta tcgcttttca tccgcgagtt atccagtaaa ccaactttat ttttgtagtt
    24181 gcgaatattg gaaattaacg agttaaaacc aatatccaga taggcataaa ttcccgttga
    24241 gcggcgctcc tcagttacca gtgcaaatcc gtggtttatg gcttcgttgg cattatggtt
    24301 attgatctgt ttgccgtgca acgtaatggt gccagccgat ttctcgcgaa taccaaataa
    24361 cgtctcaaca atatcggtac gtttcgcccc caccagcccg gcaataccga ggatctcccc
    24421 tttatgcaga tcaaacgaga catcgcgaat cgacggctgg cgcagtgacg ttaggttacg
    24481 cacctcgagg atgacttcgc ccggcttgtt ttctttgtca ggaaaacgct gattaagaga
    24541 acgcccaacc atcatggcga tgatcttgtc catcgtcagt cctgccagcg gttcggtggc
    24601 gatccactga ccgtcgcgca atacggtaac ttcatcacat aactggaaga tttcttccat
    24661 tttatgcgag atataaacaa taccgcagcc gcgctctttt aatttacgaa taatagtgaa
    24721 cagatgattg acctcttttt cggttaacga agaagtcggt tcatccataa tcacaatttt
    24781 cgcgttatag gaaaacgctt tggcgatttc gatcatctgc atttgcgaaa cggataatgt
    24841 gccgacgcgc gcacgcggat cgatatcaat atccagttca tcaaaaattg ctttggtttc
    24901 gcggtacatt ttgtcctgat cgacaaacat gcctttggtg ggatatcgcc ccagccacat
    24961 gttatccatc accgaacgtt gtaataccag gtttaactcc tggtgtacca tcgaaatacc
    25021 attttccagt gcctctttgg cggaatggaa atcgatctct ttaccctgga ataaaatggt
    25081 gccggagtct ttttgataaa taccaaacag gcattttaat aatgtcgatt ttcctgcacc
    25141 gttttccccc attaatgcat ggatagaatg tggccggact tttaaattaa cgttatcaag
    25201 tgccttaaca ccaggaaagg acttgttgat accgctcatt tccaacaagt attccccgga
    25261 agacggagtc gttgagctga ccatataatt ttaccttgtt ggccatacaa taagggcgca
    25321 gtaaacgact gcgcccaatc agtcttattt cttgctgaat tcagccaggt tgtctttatc
    25381 tacgccaaca taaggtacgc ggaccacttt gttgtcgatt ttccagttgg tgccatcagc
    25441 cgcacctttg ccatcggcca ggtttttcgc cagatcaaag gtcgctttcg cctggttgtt
    25501 agcatcgttc agtactgtgc ccgccagtgc accggatttc accagcgcca gcgcttctgg
    25561 cagcgcatcg acgccaaaca ccggaatgct ggacttgttg tgtgctttca gtgcttctac
    25621 cgcgcccatt gccatcgcat cgttgttggc gataaccact tcgattttgt tggcgttcgg
    25681 gccagacagc caggcgtcca tcttatcttt cgcctgagcg gtgtcccaca ttgcggtatc
    25741 taactgtaac tgttcagttt tgatgccttt atcgttcaat tctttaatca cgtaagtggt
    25801 acgtgcttct gcatccggat ggcccggttc acctttcagc agtacgaact gaatctgacc
    25861 gtctttgttc agatcccaac cctgattcgc cgcccagtgt ttagcaatca aatcgccctg
    25921 aataatgccg gactctttgg agtcagtgcc aacgtagtag gctttgtcgt agctatccag
    25981 cgccttacga gacggttctt tgttgaagaa aaccaccggc acgttttgcc cacgcgcttt
    26041 ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg attgccagtg ccttcacccc
    26101 tttcgccagc aatacgtcga tctgatcgtt ctgcttggac tggtcattct gagaatcatt
    26161 catcagcagc tgaacatctg gcgcggcttt cgcatcttgc tcaatagcct tgcgcactac
    26221 agacataaag ttatcgtcgt acttatagat tgttacacca atgcgagtat cagcagcgtg
    26281 tgcagcggca ccgaataaca tgctggccat cacagcagac agggttaaca ccttcttatt
    26341 catggtatct ccggtttttc ttatgcaggg tagtgcttga gataaatgct cggcggggca
    26401 gtagagttaa tgaagtgtta ctgaacgccg aagctcactt ttttaaattc gttcttccat
    26461 gcccggtaac gctccagaaa acggctttaa ttgttgttat gacgctatta cctcggtgaa
    26521 agtgattatt cactgttaca tacgggttac aacgttaaaa cggtgcaatc atagctatca
    26581 cattgttaag atactgtgaa atcactcaca gattgaaagc ggttacatcg cctgatttgt
    26641 tgagttagtg atcgccgccg cattctggcg cgttgcgaca gaatggcgac gcactaacgt
    26701 cggcataaaa cagtggctgg cacgaggatc aatattgcct gctgccccct gcaaggccag
    26761 ttcggtggct aatttagcca ttgaagcaat gggataacgc acggtcgtta attgcgggtc
    26821 ggtgtaacgg gcgatgggaa tatcatcgaa accgatgatt gagagatgta acggaatcgc
    26881 aatgccatta tcttttaatg ctgtcagtgc gccagcggcc atattgtcgt tataagcaaa
    26941 tatagcggta agttgtagat tgcgccccag cagttcaacc atcgccgcct caccgcccgg
    27001 catgtccggc gtaccagtgc caatccagct ttccggcgga ataatatcct gctctttcaa
    27061 cgcactcatc cagcctgctt tacgcatggc gtcatcttca atgccgtgac tggaagaaag
    27121 ataaccaata cgttgatgac cgttattcag caacatgcgc gtcgccattc gggcaccgct
    27181 gagattatcc aggcaaacgc aacgatgggc gtaccccggc acaacgcggt tgattaacac
    27241 cataccggga atgttatcca taaattgcgc cagttcatcg tcactcaatg cttttgagtg
    27301 aacaatcaac gcattacaac gctggcgaat taacacctca atggcgtgac gctctttttc
    27361 cgcttcatga tagctattgc cgattagcac gtatttctga tgctgctgag cgactagatc
    27421 caccgctttt accagcgcgc cgaaaaacgc atcagaaacg tccatcacca ccacgccaat
    27481 ggtgtcgcta acctgagttg ccagcgcctg ggcattggcg tttggccgat aatccagctc
    27541 actcacggct ttcattactg cttcacgcgt gtcggcactg accagcgtgc tgttattgag
    27601 cacccgggaa accgttgcca cagagacgcc agcctaacgc gctacatcac gaatggtgat
    27661 catattcact acctgttatg agattgcagt aaatgactgc ttgctggcgg ctattttgtc
    27721 agcactgaat acaggacttc gtgaatcgag tcacagcaat ggaaacggtt acagccgttt
    27781 tgttaacgaa tgtgatccag atcgttatct tgatgtttta gatattgccg ttccggcagc
    27841 acgcaaagtt aactgacgcc agagccattc caccggcccc tggcggaagt aacgcagcca
    27901 gataacagag aagaggatat tcgccagcca taccggaata acaaacgcca gcagctccag
    27961 gcggtcaaaa tgcataaaca aaccgaggtg gtaaaaaagc atggtacaaa tcagcgtttg
    28021 caatagatag ttggtcagcg ccatccgtcc gacgcaggcg atcgcaagca ccagcttaaa
    28081 gcggctcaat tgcggccaga agccataaaa cagcgacgca tagccaatcg cctgaaacgg
    28141 cgcactcagt tcccgcggca tttgaagtaa gaaggcacac cagcgatacg cccagtccag
    28201 ctgccattgc agggcgatgg caggaaggtt aatggtcacc ccaatcgcca ccagcacaaa
    28261 accagtacga cgatagtgac gtaagctgaa ctgccctttc agccagccgc tgcgcatcag
    28321 cgccgcacca atgagcatca tccctgccag ttgccagcca tactgtgcgc ccagtgccag
    28381 taaactgttg ccaacaccat cggcacgatt actgatcgct tcaacgccgc cgtgaagctt
    28441 ccagtatttt tcatacaaaa tagccgatgc atccggcgtc caggtgcggc tggtctggct
    28501 gtcggaaatc agccccaata acagcaaaac gccaagcccc accagataaa gcatgacgcc
    28561 tgtattaaac aggcttttta ccgatggcgc atcgcgcacc agccgccagc agattaagcc
    28621 caccagcccg taagccagca gaatatcgcc gtcccagaac aataaaccgt gaataaaacc
    28681 cagcaagact aacagcgtta accgcgactg gatccagcgt ctgccacggg gcagcaacat
    28741 ttgcaggccc gcaccaaaca gcagcgcaaa aagcgtgagg aatttcacct ggccgatgag
    28801 atcgagaaat gcccaggtcc atgcatcctg cggcgtaata gcgccgtacc aggcgggatt
    28861 gagataagcc gcctttggta gcccaaaggc gctgatgttt aatagcagga tccccagaat
    28921 ggcgacgccg cgaacaaaat cgagcgttac gttgcgctcc atggttcctg ccttttaatc
    28981 agttgtgatg acgcacagcg cgcagaaact cgtggcgcgt attctgactg gatttgaaca
    29041 atccaccaag agaggtcgtt gttgtggcac tggttgcatc gcggatgcca cgcgctttca
    29101 cgcagtaatg caccgcatca atggagacag ccacgttatt ggtgcccagc agcgtttgta
    29161 gcgcaataag aatttgctgc gtcagacgtt cctgcacctg cggacgctgg gcaaagaact
    29221 gcacgatacg gttaattttt gacagaccga tcaccgaatc tttcgggata taagccaccg
    29281 tcgctttgcc atcgatggta acaaaatggt gttcacaggt actggtcaga gtgatatcgc
    29341 gcacggtgac catttcatcg accttcattt tgttttcaat gagggtgatt ttcgggaagt
    29401 tggcgtaatc cagaccggag aaaatttcat cgacatacat tttagcgatg cgatgcggcg
    29461 tttccatcaa actgtcatca gccaggtcga gattcagcag ctgcatgatt tcggtcatat
    29521 gaccagcaat aaggcttttg cgcgtttcgt tatccatttc atgcacgggc gggcgcagcg
    29581 gtgtttccag tcctcgcgca actaacgctt catgaaccag ggccgcttct ttactgagtg
    29641 atggcattta tgatttctcc tgcaggtgtg acgcctccgc cctgcgtggg ggcaaagtta
    29701 ttaagctgat ttacagcctg attattgtgc gtgaggcggc gcacataatc cagtattcac
    29761 agcgataatt attgtaattg ccgctgactt tcatcagcag atgttaaaac atcgttatgc
    29821 aaatacggaa gtgaaagtta ctcacagcac attgaataaa cggtatgatg aagaaattgc
    29881 aaacaacaca acaaggagcc acgcatggaa atgctcgaag agcaccgctg ttttgaaggc
    29941 tggcagcaac gctggcgaca cgactccagt accttaaact gcccgatgac gttcagtatc
    30001 tttctcccac cccctcgtga tcacaccccg ccaccagtgc tgtactggct ttccggatta
    30061 acctgcaatg acgagaactt caccaccaag gcgggtgccc agcgcgtggc ggcagaactg
    30121 gggattgtac tggtgatgcc agacaccagc ccgcgcggcg aaaaggttgc caacgacgat
    30181 ggctacgatt taggccaggg cgcaggcttt tatcttaatg ccacgcaacc gccgtgggcg
    30241 acgcattacc ggatgtatga ttatctgcgc gatgaattac cggcgctggt tcagtcgcaa
    30301 tttaatgtca gcgaccgctg cgccattagc ggtcactcaa tgggtggtca cggtgcgctg
    30361 attatggcgc tgaaaaatcc gggtaaatac accagcgttt cggcctttgc gccaattgtg
    30421 aatccgtgca gcgtcccgtg gggaatcaaa gcgtttagca cctatttagg tgaggacaaa
    30481 aatgcatggc tggaatggga tagttgcgcg ctgatgtatg ccagtaacgc gcaggatgcg
    30541 atcccgacgc ttatcgatca gggcgataat gatcagtttc ttgccgacca gttgcaacct
    30601 gcggtattgg cagaagccgc gcgccagaaa gcgtggccga tgacgctgcg tattcagccg
    30661 ggatatgatc acagttacta cttcatcgcc tcttttatag aggatcacct gcgcttccat
    30721 gcgcagtatt tactgaagtg aaagtccgcc cggttcgccg ggcatcttct catcagaagc
    30781 gataatccac tgccataaag taacgacgtc cgtcttcgtt atagctgtag tcgtcacgac
    30841 tgagatcctt gtcgccaagg ttcagcacgc ctgcgcgcag tttgacgtct ttagtcacct
    30901 gccaggccgc gccggtattc cagatggtat aaccgcccgg tgttttagcc gtcgcgctgt
    30961 cggcgcgttt ctgcccggta tagtgaccag aaacatagaa tgaccagtct tccagcgcca
    31021 gcggtttcca gtccagcgta ccgttagcag tatggaacgg cagatcggat agcggtttgt
    31081 tttcgccgtt gctgacatca cgaccatcgt tgtaggtgta gttgatcgac agtttccatt
    31141 catcgttgaa cggaattttc agttcggttt ccacgccctg aatacgagct ttgttaacgt
    31201 tgtagtagct aaataccggt atgcgccgtc cgttagcgcc cgtctcaaaa ccaacaaagt
    31261 tttggtagcc cggtgcagcg ttgacgtcag acgtacggct gatgctgata cgatctttca
    31321 catcgttacg gaaaacggta acgctggatt caaccccttc cagccagcct tcttcaccca
    31381 tgtagtaaag ccccagctcc caactttcgc tggtttctgg tttcagatcc gggctaccca
    31441 caatcttaca tgcgccacgg caggaattgc tcgtccagtc agggctaagt tgcaacagag
    31501 aaggtgcttt aaatgccgtc gcccagcccc ctttcaccgt tacggtgtcg gtggcgttat
    31561 aaaccaggta ggcacgcgga ctccagtgtt caccgtaggt ttcgtgatcg tccatacgca
    31621 cgccggtcgt cagcgccagc ggctcgaaga tccgccattc atcttccaca aacagcgcgt
    31681 actggctggc agacgttttg gagctggttc ccccggtcag gttcaccgca tcgctaagtt
    31741 tgtcgtgacg ccattcaccg ccaaccgtga gaaactgatt aatcgccgtc agcggcaacg
    31801 tgtatttgcc gtcgaccgta ttgctttcgg aagttatcgg gctgctgttg ccagggtttt
    31861 tgttctcgac tttctcaccg tagtatttca gttcgctggt gccgtaatcc caacgcccat
    31921 tatggctgac ggagtagttc tggcgttcca ggcggttttt gtccagcgag tcggaatcac
    31981 gatcctgacg gtcgaaaccg tatccggcag taaaatcgtg attttgattc ggtgtccagg
    32041 caaattcgac attgccgtcg cggctggaga atccttcaat acgcggcgtt tctccggtat
    32101 cggtggtcgt tgagttttgc gggtcatcct tttcacgttt tgccaggctg ccgtaagctt
    32161 tcattcccag cacaccatca attaatggtc cactggtaaa gaactgaccg ttataggtat
    32221 caccgcgatc gcgatgttcc tgaatggtgg tatcgacggt aacggtaccc gaccatttct
    32281 gaccgatttt tttggtgatg atattcacta caccgccgag cgcatcggaa ccgtacagcg
    32341 acgacatcgg gccacggacc acttcaatac gttcgatgga atcgaccggg atccagttca
    32401 gatcgaaatc attgtggcgg aagacggcat tgcgggagtt cacgcgttta ccgtcgacga
    32461 gaatcagggt atagctgctg tccagaccac gaatactaac gcccttacgg ttatcccctt
    32521 cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc cttcagattc tgtaccggtt
    32581 ttcgctgcag atcttcctgg gtaatgacgc tgatactggc gggtgcatct ttaaggtttt
    32641 gttcaacgga agatgcagtg acaaccatcg tttcgccatc gtcatcgacc gctaacacag
    32701 gccatacaca agaaatagcg gacaaacaca gcccgacccg tacgaaaggg ttcaacctaa
    32761 acattccata tctccatgag gtaactacga aaataaaatg ggtatcgctc acatcttctt
    32821 tacgtcccct tgcgtacggc agcatcgcgg tggacttatt tgtatgcagg tgatcatccc
    32881 agaaagccgg accagcctct gatttgtcag ccttttttga ttgtagcgta acgataatgc
    32941 aaacgataac aattatcaat ccaaatgtta aattttatat ccgcatgatt tgtcagggaa
    33001 atggaattga aaaagccctc tcggttgaga gggcttagca aggaagggag gaaacttatt
    33061 tcttatcgtt ctgcgggaac ttcatttcgc tgtagcgtac gaagtgagtt cctttaatca
    33121 gcttgtagcc gaaccaaata atcaggaaca gcgggatacc aatatacgtt gccgctacgc
    33181 cgccccagtc aatggtatct ttcaggaacg cttcgtagtt ctggcccaaa gtgataatca
    33241 gacacagaat gaatgcgaag atcggcccca gtgggaagaa acctgaacgg tacggcagat
    33301 cgttaatgtc gtgtccctgc aatacgtaac cgcgacggaa gcgatagtgg ctaatggcaa
    33361 tccccagcca ggcgataaaa cccgtcatcc cggaggtgtt cagcagccac aggtataccg
    33421 tctggttgcc aaacatggag gtcaggaagc acaaaccggc aatcaccgtc gtcgcataca
    33481 gcgcattacg cggcacgcca ccacgcgaca atttagcgaa aatgcgcggc gctttaccgt
    33541 cacacgccag ggtgtacagc atacgagtag acgcatacat accggagtta cctgctgaca
    33601 gcaccgccgt cagaataact gcgttcatca ccgccgccgc agagagcaga cccgcgtgct
    33661 ggaacaccaa ggtgaacgga ctgacgctga tgtctttaac atcgttacgc agcaggctcg
    33721 gatcggtata cggaataatc aggctgataa tcaggatcgc gaacacatag aacaacagga
    33781 ttcgccagaa tacctgacgt accgcgcgtg gaatgttttt cgccggatct tcggactcgc
    33841 ctgcagcaat accgatcagc tcggttccct ggaaagagaa gccgacaatc atagctacgc
    33901 cgatcatcgc cgcaaaacca ccagcaaacg gcgcttcgcc gattgtccag ttgctccagc
    33961 ccgcaggctg cgcgcctttg aagataccga taatcatcag cacgccaacg atgataaaga
    34021 caataactgt cgtgactttg atcagtgaaa accagtattc cgcttcacca aagccacgaa
    34081 ctgagatgta gttcagcagg aagataacgc cgaggaacaa cgcactccag atccagcccg
    34141 gtgtatccgg gaaccaccag ctcatgacca gctgtgctgc aaccaagtca acggcaatag
    34201 tcaccgccca gttgtaccag tagttccagc ccagcgcgaa gccaaagcct tcttcaacat
    34261 agttctgacc gtaagtggca aacgaaccgg aaaccggcat atatgcagcc aattcaccga
    34321 gactggtcat caggaagtaa accatcaggc caatcagcat atacgagagc aatgccccgc
    34381 ccggacctgc ctgagaaatc gttgcgccag aggcaacaaa aagacctgta ccgatggaac
    34441 cgccaatggc aatcatcgtc aggtgacgcg cctttaattc acggcgtaag cccggcgctt
    34501 ctgtagtttt agtttcggaa cccatacaaa aatgctatcc atcttaaaaa tgaggcgcga
    34561 ttgtagcaga cgattggcaa tccttccggc agaaatacac ggttataaga caccttcatg
    34621 atcgcccagg gattataagt aaagcagcca atctcttttc tggcgaatgc atgctgaggg
    34681 cgctgcgtca cgaaatcgac acagcaccag catgttcttg tacagcacct taacgcggta
    34741 cattcgcggg atcgcaatag tccagaaagc gccgtagcgc gttggaaagg tgtttttgcc
    34801 gatgatgtat acgccacaac gtacgcatca ggcgcgggag agggaccgca acttcactta
    34861 atatgcctgc ctgcaattga tcttcaatca cacgtcgcga caggcaacta attcccaacc
    34921 catgacgcac cgcatgtttg attgcctcgg agttacctaa ttccatcgcc atctcaaact
    34981 tcggtaaatg tgacagcaac agataatcga caatctcccg cgtgccggaa ccgcgttcac
    35041 gcaggatcca cggcgcagcg gccagctgtt ctaaggtgac cggaccacga gccaacggcg
    35101 aagtcggcgc ggcgaaaacc accagctcgt cttccagcca cggttcagaa atgatttcag
    35161 tgctgtggca cggtccttca ataaagccaa tatcaacgcg gaaatccagc accgcttgca
    35221 tcacgtcctg gctgttccca acgctaagtt caatcggcaa ctgcggataa tcatggcgat
    35281 aacgggcgat aactgcaggc agaatgtagt taccgatggt actactggca tagatacgaa
    35341 tcgcgccgtt gtcttcgcga aacagttgtt cgatttcaac cgcctgttca agcaatgcca
    35401 acgcacgcgg atagagcagc cgcccgtgtt cattaacaac cagtcttttc cccacgcgat
    35461 caaacagttg cacgccaagc tgcccttcca ggtcggtcaa ggctgcgctc actgctgatt
    35521 gcgacaacgc cagcatcacc gacgcctggg tggttgatcc acttttcaat acttctgcaa
    35581 aaacttccaa ctgccggagg gtgatgtgca tagtcgctta ccacttataa agattaatta
    35641 taaatatata atcaatttta tttttaaacc agttagtcgt aaccttatgc ccggttaaag
    35701 gagagggtta tgacgaatat caccttacag aaacaacatc gtacactgtg gcattttatt
    35761 ccggggttag ccctgagtgc agttatcacc ggggtcgccc tgtggggtgg ttccattccc
    35821 gcggttgcgg gtgccgggtt tagtgccctc accctcgcaa tcttgttggg gatggtttta
    35881 ggcaacacca tctatccgca catctggaaa agctgtgacg gtggtgtgct gtttgccaag
    35941 caatatttat tacgtctggg tatcattctt tatggcttcc gtctgacgtt ctcgcaaatt
    36001 gccgatgtcg gtatcagtgg gatcatcatt gacgtgttga cgctgtccag taccttcctg
    36061 ctggcttgct tcctggggca gaaagtgttt ggtctggata agcacaccag ctggttgatc
    36121 ggtgccggta gcagtatctg tggtgctgcc gcggtactgg cgactgagtc ggtagtgaaa
    36181 gcggaagcca gtaaagtaac cgtggctgtt gcaaccgttg ttatcttcgg gaccgtcgcg
    36241 attttcctct acccggcgat atatccgctg atgtctcaat ggtttagtcc ggaaactttc
    36301 ggtatctaca ttggttctac tgtgcacgaa gtggcgcagg tggtggcggc aggtcatgcc
    36361 atcagcccgg atgcggaaaa cgcagcagtt atttccaaaa tgctgcgcgt gatgatgctg
    36421 gctccgttcc tcatcctgct ggcggcgcgt gttaaacagc tgtctggggc gaacagcggc
    36481 gagaaaagca aaatcactat tccgtggttt gccatcttgt tcatcgtcgt tgccatcttt
    36541 aactcgttcc acctgttacc gcagagcgtg gtgaacatgc tggtaacgct ggataccttc
    36601 ctgctggcaa tggcgatggc ggcgctgggt ctgaccactc acgtcagcgc gctgaaaaaa
    36661 gctggggcga aacctctgct gatggcactg gtactgtttg cctggctgat tgttggtggt
    36721 ggtgcgatta actatgtgat tcaaagcgtc atcgcataaa ccactacatc ttgctcctgt
    36781 taacccgcta tcattaccgt tttcctccag cgggtttaac aggagtcctc gcatgaaata
    36841 cattggagcg cacgttagtg ctgctggcgg tctggcaaat gccgcaattc gcgccgtcga
    36901 aatcgacgca accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc gtgccgcacc
    36961 gctcacgacg caaaccatcg atgaattcaa agccgcctgt gaaaaatatc actacacatc
    37021 ggcgcaaatt cttccccacg acagttatct gattaacctc ggacatccgg tcactgaagc
    37081 tctggaaaaa tcgcgcgatg cctttataga tgaaatgcag cgttgcgaac aactggggct
    37141 ttctttgctc aacttccacc ctggcagcca tctgatgcag atttcagaag aggattgcct
    37201 tgcgcgtatt gccgaatcca tcaacattgc gctggataaa actcaaggtg taacagcggt
    37261 gattgaaaac accgccggtc agggcagtaa cttagggttt aaattcgaac atctcgcggc
    37321 gattatcgac ggcgtggaag ataaattccg cgtcggcgtc tgcattgata cctgccatgc
    37381 tttcgctgcc gggtatgatt tgcgtactcc agccgaatgc gaaaaaacgt tcgctgattt
    37441 tgcccgtatt gtcggcttta agtatctgcg cgggatgcac cttaacgatg cgaaaagtac
    37501 ctttggcagc cgcgttgacc gccatcatag cctcggtgaa ggcaatatcg gtcatgatgc
    37561 gttccgctgg atcatgcaag acgaccgttt cgacggcatt ccgctgatcc ttgaaaccat
    37621 caacccggat atctgggcag aagagatcgc ctggctgaaa gcgcaacaaa ctgaaaaagc
    37681 ggtggcctga tcgatgaacg acagggaaaa gcagattctt aaaattttac ggcgtaaccc
    37741 gctgattcaa cagaatgaaa tcgccgatat tctgcaaatc agccgctcac gtgttgccgc
    37801 tcatattatg gatttgatgc gcaagggttt gattaaaggc aaaggatata tccttaccga
    37861 gcaggattac tgtgtggttg tgggtgcaat caatatggat attcgcggaa tggcggatat
    37921 ccgttacccg caagcagcct ctcatccagg ttctgttcac tgttccgcag gcggcgtcgg
    37981 acgtaatatc gcgcataacc tggctctgct tggtcgggat gtgcatttaa tatcagctat
    38041 tggcaacgat ttttacggcg agacattact ggaagaaacg cgccgcgcgg gcgtgaacgt
    38101 ttcaaactgc attcgtctgc atggacacag cactgcaact tatctcgcta tcgctaataa
    38161 gcaggaagaa actatcctgg caatcaatga cacacatatt ctgcaacagc taacgcctca
    38221 actgctcaat acttcacggg acttgattcg ccatgctggt gtggtgctgg ctgattgtaa
    38281 tctcacacct gaagccctgg agtgggtctt taccattgct gatgaaattc cgatgtttgt
    38341 cgataccgtt tccgaattta aagcgaacaa agtaaaaaac tggtacagcc ggatccacac
    38401 tctgaaacca acacaaaatg aactggaaat tttgtggggg caaccgatta aagacgacaa
    38461 cgatcgcatc agagctgtaa attcgctgca tcaacaaggt gttaaacgca tttttgttta
    38521 tctgaaagat gaatctgtat tttgcagcga taaggatgga gaacaatttt tgctgaccgc
    38581 gccagcgcat acgacagtag acagttttgg tgctgacgat ggttttatgg cgggcctggt
    38641 atatagcttt cttgaaggat acagtttccg cgacagcgcc cgttttgcgg tagcctgcgc
    38701 ggcaatttcg cgcgccagcg gcagcttaaa caaccctacc ctgtctgccg ataacgcgct
    38761 ttcattagtg ccaatggtgt aacaatgttg ccggattcga cgcttgaccg catcttatcc
    38821 ggctcataaa ttgcgcacta cgctaaacca ataaagaatc ctgcaatagt agcactcatc
    38881 aggttagaaa gtgtcgccgc cgccagcgcg cgtaaaccaa gttgggcgat ttccggcgca
    38941 cggtgtggcg caaccgcaga aaacgccccc accaccaccc cgatagaacc aaagttagcg
    39001 aaaccgcaca acgcgaaaga aataatcgcc acggttttag catccagagt gccacccgtt
    39061 tgcagatatg gcgagaaatt gagataagcg acaaattcat tgatcgccag cttctgccca
    39121 atcaaactcc cggcaagatt tgcatcactc cagtcaaccc ccatcaccca cgccaatggg
    39181 gccaacaggt aacctaaaat cgactccagc gaagcatgtt caaaaccaaa ccagccgcca
    39241 acaccgccga taataccgtt aatcaacgca attattgcaa caaatgccat aacaactgtc
    39301 gctacaccgg cggcgatttt cagcccggtc attgcccctg tcgcagcggc ttcaataatg
    39361 ctttttggcg gtgtttcggt gaaagagagg ttattaaaag aaacctgcga agattccgta
    39421 gccgggctta acaggcgggc aaacaagatc ccgccaggga tcgccattaa cgatgccgcc
    39481 agcaaatatt ccacaggtac gcccagtgcg gcgtaaccaa tcattgtcga accagcaatc
    39541 gaggccatgc cactacaaat cgctgtaaat aattcattgc gattcagacg atcgataaag
    39601 ggtttgacga tcgccggaat ttcgttttgc ccgaggaaaa tggtggtgac cgcgacgaat
    39661 gactcgatct tgctgatatt taaagctttc tggaagatac cgccgagaat gcgaattaag
    39721 atccccatca caccgatgta gtagagaata ctcaccagtg cagtgacgaa gataattgcc
    39781 ggtaataccc tgaaaccaaa gataaatcct gcgccatcaa ataaggtatc cattttcggt
    39841 ccgaccagag aaccgaagat aaatgcgcta cctgcgtcgc tgtacgccat cactttgtgg
    39901 acgccaaaag cgactttttc tgcgacccaa cgccctggcg gtaaccaaag cataatcccg
    39961 ccaatcacga cctgtaacac taacgccgcg ccaacggtac gcaggctgat cttcttcttg
    40021 tttactgaca gtaaaaacgc aattgccagc agtaccacca ttcccagaac acttctcatg
    40081 acatccatag tgattatctc ttcatgtcat tttgggcgac ggctttctgc ccatcagaaa
    40141 tagccgggcc tgcatcttgc gatactgtcc cggcttgtta tcaacactta atgggttttt
    40201 atgtagccgc gtacgcactc ttcgactaat ccccagaacc agtcggtatc aatagtgatg
    40261 ccgactttag tattggcggg cttgccaaga acgcccagct cgtcgcagac ggtacgccca
    40321 tagcaagggc cactgttgac gtccacttcg acgtacatct cctgggtttt aatgccatca
    40381 gggttaatca gataaccgat gcaggtggcg tcgtgcaccg ggccgccagc caggccgtag
    40441 ttttcgaact gcgttttgag agtgaagttc atgatgtcgc tgaacatctc tccggcaggg
    40501 ccgcctgccc tttccattcg tgcaattacg tccggggtgc aaacggtctg gttggtaaga
    40561 tcgaggccca tcatcactaa tggaacgccg gaggtgaaca ccacgcgtgc ggcttccggg
    40621 tcggcaaaga tattgaactc ggcagatggg gtgaagttgc ctgtaccgta agcgccgccc
    40681 attagcacaa tttcacggat tttgggcagg attgcgggtt gcatacgcat tgccaccgcg
    40741 atatttgaaa gcggaccaac cggcaccaga gtgatatcgc catcgcttgc cataagggta
    40801 tcaatgatgt atttcaccgc gtgggtactt tctgcctggc gggtcagcgg ctcgaatacc
    40861 gggccatcca gtccggtttc accgtgaata ttatcggcaa cgatttgttg acgcataatg
    40921 ggttgcggca tccccgcata aaccggaaca ttaatctcca gtttctggca aacattcagg
    40981 ccattaatta atgttttatc aagcgtctga ttacccgcaa caatggtgat acctaataaa
    41041 tcaatcgccg gatgtttcgc cgccatcatt atagcaatag catcatcatg acccggatca
    41101 caatccagaa taatttttct cttttccatt gtttatttcc tctgtttcca gttgcgttat
    41161 tttttctaca gcaacgaaat ttcgatcggg ccgatgattg aatcttaaca acagcgtacg
    41221 aatgctaaat atgagaaatc tcatagcgga taaacatcgt gaaagaaatc cacaataatg
    41281 atcttaagca gcaattgatg agtgaatccg cgtttaagga ttgcttttta acggatgttt
    41341 cagccgatac gcggctgttt cattttttag cgcgtgacta cattgtgcag gaagggcaac
    41401 agccgtcctg gctgttttac ctgacgcgag gccgcgccag gctttacgcc acgctagcta
    41461 atggtcgcgt gtcgctgatc gatttctttg ccgccccctg ttttattggc gagattgagt
    41521 taatcgacaa agaccatgaa ccgcgtgcgg tgcaggctat tgaagagtgt tggtgccttg
    41581 cgctccctat gaaacattac cgcccgctgt tattaaacga cacgctattt ttacgaaaac
    41641 tctgcgtcac cttaagtcat aaaaattatc gtaatattgt ttctttaact cagaatcaat
    41701 catttccgtt agtgaatcgc ctggcagcat ttatattact ctcgcaggaa ggtgatcttt
    41761 atcacgaaaa gcatacgcaa gcggcagagt atttaggcgt ttcttatcga catcttttat
    41821 atgttctcgc gcaattcatt cacgacggtt tattaattaa aagcaagaaa gggtatctaa
    41881 ttaaaaacag aaagcagttg tcaggactgg cgctggagat ggacccggag aataaattct
    41941 ccgggatgat gcagtaaaaa ttattggcaa tagcacgatt gccggatgca acgcttaata
    42001 aggcgtacat ccggcaatga aactcaagct aaaccaataa agaacccggc aatggtcgcg
    42061 ctcatcaggt tggaaagcgt cgccgccgcc agcgcccgta aaccaagctg ggcgatttcc
    42121 ggcgcgcgtt ttggcgaaat agccgaaaac gctccaacga caacaccgat agaaccaaag
    42181 tttgcaaacc cacagagggc gaacgagata atggcaatgg tcttgacatc cagcgtgccg
    42241 ctcgtttgca gatagggcga gaaattgaga taagcaacaa actcattaat cgccagtttc
    42301 tgcccaatca aacttccggc aagatttgca tcactccagt ccacccccat aatccatgcc
    42361 aacggggcca gcacatagcc gaaaatgctt tccagagaga cattggcaaa accaaaccag
    42421 ccgccaactc caccaataat gccgttgatc agcgcaatga tcgctacaaa tgccattacc
    42481 accgtcgcca caccggcggc gattttcagc ccggtcattg caccgttcgc cgcagcttca
    42541 ataatgcttt ttggcggtgt ttcgctaaac gacagatttt caaacgtaac ttgcgaaggt
    42601 tccgtcgccg gactaagaat acgtgcaaac aggataccgc caggaatagc catcagcgaa
    42661 gcggctaaca aatagtcaat cggcaccccc attccggcat agccaatcat catcgaaccc
    42721 gcaatagacg ccatgccgct acaaatagcc gtaaatagct cgttgcgatt catgcgatca
    42781 ataaaaggtt taacgatcgc cgggatctca ttttgcccga ggaaaatagt ggtgactgcg
    42841 acaaaagatt cgattttgct gatgttgagg gctttctgaa aaatactgcc aagaatacga
    42901 atcagcagcc ccattacgcc aatgtagtac agcaagctga taagcgcggt aacgaaaata
    42961 atcgccggga gtacgcgaaa ggcgaagata aagcccgcac cgtcaaacag aacatccatt
    43021 ttgggaccga ccagcgaacc aaaaataaag gcacttcctg cgtcactgta ggacatcact
    43081 ttatggacgc ctaatgccgc ctgctctact gcccattttc ccgggggaaa atagagcatg
    43141 atgccaccaa tggcaatttg cagcagcagc gcagcgccaa ccgtgcgcaa gctgatgctc
    43201 tttttattta ccgacaacac aaaggcaatt gccagcaata ccaccatccc cgcaacactt
    43261 ctcattatat ccataatgat tttcccttca tgccagtaaa cccggcgtca gcgccaggtt
    43321 ttggtatgtt tgatgagtac gggcgacggc tttctgcccg ccagaaatta acccacgaga
    43381 cgctgatatt ctttggcaat ttcgctcgcc agaatggcgt tattaaagac cagttgaata
    43441 ttggatttca ggctatcacc gccggttagc tccgcaacac gcgccagcag gaacggtgta
    43501 ctctctttcc caataacacc ctgttcttcg gcttcagcaa cggcttgatc aattgctgca
    43561 ttgatggttt cttctggcat cgcaaactgt tccgggatcg ggttcgctac caccaggccg
    43621 ccgttcaaac cggtttgcca cttcactgcc atcgcgcgag caatttcgcg ggcactgtca
    43681 agacgaatgc tgacttcaaa tgggctggtt cggcagaaaa acgcaggcag cgcttttgtc
    43741 tgataaccaa ttaacggtac accgaaggtt tctaaatatt ctgttgttaa tcctaaatcg
    43801 agaatagatt ttgcccccgc acaaacaaca gtgacattgg tatttgccag ttcctgtaaa
    43861 tcggcagaaa tatcgaaggt atgttccgct ccgcgatgca caccaccaat acctccggtt
    43921 gcaaaaactt taatcccagc cagtgctgca ataatcatgg ttgatgcaac ggtagtcgcg
    43981 ccatttttcc ctgcagcaac gacaaaaggt aaatcacgac gactaacttt cgtcacgtta
    44041 tgtccttcac ggcccagcaa ttcgatttct tctttactta agcccacttt catcacaccg
    44101 ccaataatgg cgatagtggc tggtactgcg ccctgtttac gaattgtttc ttctacttca
    44161 attgcggttt gggcattttg tgggaatggc attccgtgag aaattatggt cgattccagc
    44221 gcaacaaccg gttttttatt ttttaaagca tcctgtactt cagcggatat ttgtaataat
    44281 tcaggggaca gagttaattc agacattctg tgttctccac taacgatcta acattggcaa
    44341 ttgataattc agggttgttg gtatattcac aggcaagtgc cattgacgaa catccctgcg
    44401 caaatcgaac agaatcgata aacggcatac cgtctaccca acaggaggcc agccccgcca
    44461 tcatggcgtc gcctgcccca gtaacattga tgacattcgt tttaataggg gccgaccacc
    44521 cactttcacc gttgatatca ctgtagtaaa cgccatcccc tcccatgctc agcaccaagc
    44581 ggttcaggcc gtgttgatga aaccaggcgg cgacctttgc gacatcttca cgtccggaaa
    44641 gtgcaattcc gctcaacgtt tcggcttcaa gacggttagg ttttaacgta tggattttgc
    44701 tcagatgatc gcggattttg acgcatttcc atgccgatac cggatcaata aatacaggcg
    44761 tttccccgct attttccaga acccagacca gcacctcttc actaaggttg caatccgcga
    44821 caataacctt tgcaccgcga ataaactcct gatgctgggc aagaaattct gcagaaatac
    44881 agtcactaat actcatatca tttatcgcga caagcatttc tcctgtatta tcgagcagtg
    44941 ataaatagct ggatgtattc tcacccggaa caattaagca tttatcgaca taaacaccgg
    45001 attgattagt ttgcgccagt aatgattgcc cataaaaatc accacctact gcacttaaaa
    45061 gccaggcatt tttccccagt aacgccagat tatgagcgat attccttccc acgccacctg
    45121 gggtaaattt aatttttccg ggattcgaat ccgcataatt caatggatga tgtgaatacc
    45181 cagccacatc aatattcgcc gaaccaataa ttactatgta gtccttttcg cgcatagcac
    45241 gccccctggc aataaattgc caacgaagaa attgggtaaa atagccatta cagtcaataa
    45301 gactattaga gcacgtgttc agatttaaac acatgttcat aataagcata tttaacgtaa
    45361 tgtaaatcga tcagaaaaaa cgaaatcggc cagatatgaa ggcggtcaca aagtttgtct
    45421 atttatgcag caacaaaaga gaaagttgtg atgtgtaaca cacaatatgg caggcaagaa
    45481 aaagggcaga gatgtttctg ccctccagat tgttgacaac gcgcgctttg ttcatcctgg
    45541 atgtggcgta aacgccttat ccgacctaca aaagcgcaaa aaattcaata tattgcaggg
    45601 ttcatgtagg tctgataagc atagcgcatc aggcaatttt gcgtttgtca tcatcggggc
    45661 agagatgtct catccccgat aatgaagata aattatgcgt ttttttctac tatttgtgtt
    45721 tccgggcgtt tcaggaaggc ataggccaaa cccgccacca gcgtaccggc aataattgct
    45781 accaggtaac ccaataccgg cgtaatagcg ccagggatca gcagaacaaa cagaccaccg
    45841 tgtggtgcca tcagtttcgc accaatcgcc attgagattg cgccagtcag cgccccacca
    45901 acgatacagc acggtagcac acgcatcgga tcacgagcag caaacggaat tgcaccttcc
    45961 gaaataaagc acagaccaag taccagagcg gctttgccac cttcctgctg cgctttgtcg
    46021 aatttgcgac gcgccaccat tgttgccaga cccattgcca gcggtggcac catacctgcc
    46081 gccataatcg ccgccatcgg gccataggtt tgagtactca gcagacccac accgaatgcg
    46141 tacgctgctt tgtttaccgg accgcccatg tcagtacaca tcatgccacc gaggatcgcc
    46201 cccagcagaa ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa cccatcgaga
    46261 atgccagcaa ccggtttacc gattaggtag atcatcgcca gaccgaccac cagactggaa
    46321 attagcggaa tgatcaggat cggtttcagc gcctccatac tctgtggcag tttcaattgc
    46381 gtactgatta acttcgcaat gtaaccagcc aggaagccag caattatacc gccaataaag
    46441 ccagaaccgg tgctgaccgc cagcataccg ccaatcagac ccggtgtgag gcccggacga
    46501 tcggcaatgg agaaggcaat ataacctgcc agtaccggca ccatcagcgc aaaggctgaa
    46561 ccaccaccaa tctgcatcag cgccgctgcc aacgtacccg gctctttaaa cgcttcgata
    46621 ccaaaagcaa aagaaagcgc gatacacaga ccacctgcaa caaccatcgg cagcatgtaa
    46681 gagacgcccg tcagcaagtg acggtaagcg cctgcactct ctttcttacc ttcagtggtc
    46741 gctgtttgag ctttgcccgc tggttcatac ggcgttgctt cagcaaccgc tttatccagt
    46801 tcctgcgcag ttttcttcag cgccagaccg gtagaggtgc gatacatcgg tttaccagca
    46861 aatttcgcca gatccacttc gatatctgcc gccacaatca ccagatccgc tgctgcgact
    46921 tcttcagggg tgattgcatt gcccgcgcca acagaaccac gggtttcaac tttcacccac
    46981 cagccacgtt ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt gtgtgctacg
    47041 ccagtcgggc aagcagtcac cgcaactacg cgtttcggac cgctggcagc aaccggtgct
    47101 gtcgcagcga ccggcgcagt gtaaggtttc gcatggcctt tggcttcact caggaacagc
    47161 tcagggtgcg caactgcccg ggaaatatca cccagccaga catttttacc gttcagcgcg
    47221 ctgtcattcg ggatggaatc accgagaaca atcgccattt ctgcgtcgtt cggattgtcg
    47281 atgatttcca gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc catataggcg
    47341 cgtgcctgac cgagattagc gtcaataatc agcagcgttt tcattatgcc tctcctgctg
    47401 tcagttaaaa ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg gacgatcggt
    47461 aatacccaca ttgctttgac ttaccgccag ggctgcaaca gctgtcgcca gacgcagtgt
    47521 gtgttcactg gattcacgca tcagcaagcc ataaatcagg ccaccaacca tagaatcccc
    47581 tgcgccaacg gtgcttacga catcgactga cggtggtttg gcgatccatt cgccggaggc
    47641 attaacccaa agcgcgcctt cggcacccag tgaaataaca acatgcgcaa taccttgttc
    47701 acgtagcgca tgtgcagctt caatcacatc tttcatttca ggcagtttac ggcctgccca
    47761 gatttccaac tcgcggcggt taggtttcac cagccacggt gccgctttca aacctgctac
    47821 taacgcttca cggctactat caaagataat gcaaggacac tgactacgca ggcgagtcat
    47881 ccagtcggtg aacgcttccg ggctgacgcc tgacggtaag cttccgctga cacagaccat
    47941 atcgaactga ccgagccagc tcagagaatc agtcacaaag cgttcccagt cggcgagggt
    48001 gacttcaaaa cccgagaagt tgaagtcggt cacttcgccg tctttttccg tcagcttaac
    48061 gttaattcgg gtgcgcccct gtacaacctg gaaacggttg gcaatgccca gctcgctgaa
    48121 cagttgctga aaaccatcct gattgtcttt acccaggaag ccgccaacgg tgacatcaat
    48181 tcccaggtct tttaatactt tggccacgtt gatgccttta cccgccgcat gcagaccggt
    48241 ggttttcacc aggttcactt cgccgcgttc aatttccggg cagaaaccaa caaggtcata
    48301 agccggatta agggtgatcg tagcaacacg tctgctcatt atgcgccctc cccaagacca
    48361 gcagcgatag cgtcgccaat ggctttcagc gcctgttcag catctgcacc ctgggcggta
    48421 aagcgtaggc gatgaccttt cttaacgcca agtgccacaa ctttcatcag actacgtccg
    48481 tttgccggtt tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt aaattgttta
    48541 atggtattga ccagcatggt acctggacga gcatgcaggc cgtgttcatt gcgcaccaca
    48601 aactccgcgc ttaacacgtc gtcggtcggc gcatcatcgc tggtcagcag cgccagcaac
    48661 gttgccgcat ccgctttcag caagcggtca gctttattgt cgagcaataa atcagcgaga
    48721 cgcttaagaa ccgcgatggg ctgatcgtca ttcatcgcca cactcaccag catggctgcc
    48781 gtttcgccgt ccacatcaaa agcatttgcc gcacggctta ccgcaatcgc gctacgcaga
    48841 ttgccttcgg cgctatcgct cagccagata ccctgtccga gattcagcgg ttgttcattg
    48901 atggctttgg tgacgaaagt ggcgtcaact gcccccacct ctttcagacg cgcagcgttc
    48961 agcgcctgaa gagtcagcag atcgctggcg acgatatcca gcgtcagcat ttcgttgtcg
    49021 agcttcagct gctcactctg cttttcgccc atcagtaatg cgcgaagttc ttctgctgtt
    49081 gttgctgact tcagttgttc agcaacggaa tcatcgctca gtacgtgggt cagctggcgt
    49141 agcaggccca gatgttcatc cgagctggca gcaataccga ttgccacgta cgctacctga
    49201 ccgtcacccc aggtgacgcc ttccgggaac tgaaatacct gaacgccggt tttcagcacc
    49261 tgatcgcggg tgtcggtagt gccgtgtgga atagcaatac cattgccgag gaacgttgag
    49321 gtttgctgtt cgcgcgccag catgccattg acgtagcctt ctgctacatt accggcctgc
    49381 accagcgccg cagcgacctg gcgaatcgcc tcttctttgt ctccggcctt ttcgcccgga
    49441 tggatgtcct gtacggataa ctggaacata gttctcctct cttgctgaat tgaaacgatt
    49501 cagcctctat gagaaaaaaa gcgccaacct ggcttagggt taaagacaag atcgcgctga
    49561 aacgtttcaa gaaagcataa tacttctgtt tcagcacgca aggaaagccg taaagttatg
    49621 agcaaaagtt tgatgtgctg cacaattttt tgtcattttt cctaattgct gatgggaaaa
    49681 tccgttatca gcaatttcat ttcagcagcg tcagcagcca atcagcaaca gtgaaatggc
    49741 attttgatta ttcgtggcgc ttttgatttc gctgtctgtt tattttctga caagcagcgt
    49801 aaactccgcg tcttcctctt ccagtgatcg acctgcatgc ataactcccc cgcagtctcc
    49861 agcgcgaaat cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt tctcaccggt
    49921 attgcgggtg ctctgcaaac cccgacactc agtattttcc ttaccgatga agtacatgcc
    49981 cgtccggcga tggtgggatt cttctttacc ggcagcgctg tcattgggat tctggtaagt
    50041 cagtttctcg ctgggcgctc tgataagcgc ggcgatcgca aatcgctgat tgtcttttgc
    50101 tgcctgttag gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta ctttgttttg
    50161 ctattcgttg gcgtctttct tagcagcttt ggctcgaccg ctaacccgca aatgtttgcc
    50221 cttgcccgtg aacatgccga caaaaccgga cgtgaggcgg tgatgttcag ctctttttta
    50281 cgcgctcagg tttcactggc atgggtcatt ggcccaccgc tggcttatgc gttagcgatg
    50341 ggtttcagct ttacggtaat gtatctgagc gcagcggtag cgtttattgt ttgcggcgtg
    50401 atggtgtggc tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac cggcacggtt
    50461 gaagcgccgc gccgtaaccg tcgcgatacg ctactgctgt ttgtcatttg tacattgatg
    50521 tggggctcga acagcctgta catcatcaac atgccgctat ttattatcaa cgaactgcat
    50581 cttcccgaga aactggccga tgtgatgatg gggaccgccg ccgggctgga aatcccgacg
    50641 atgttgattg ccggatattt cgccaaacgt ctgggtaagc gtttcttaat gcgcgttgct
    50701 gccgtgggtg gcgtctgttt ttacgcagga atgctgatgg cgcattcacc tgtcattctg
    50761 ttgggcttgc agctgctaaa tgctattttt attggcattc tgggcggcat cgggatgctc
    50821 tattttcagg atctgatgcc cggtcaggcg ggttcagcca ccacgctcta taccaacact
    50881 tcgcgcgtgg gctggatcat cgcaggatca gtggcgggca tcgtcgccga aatctggaat
    50941 tatcacgctg tgttctggtt tgcgatggtg atgattatcg ccactctgtt ttgcttactg
    51001 cggattaaag atgtttaagg cgcggtcagc atttccagat cgataagcca ggtcatggcc
    51061 tgctgacgcg agttaccgca catttcagcg ctggcttgta agcctgcaca gactttcggt
    51121 cgcagtggcg aggtgaaaat tttacagcgt tgctgttcat caagctgaat gcagggcgta
    51181 ttggcgggct tgccatctgg cataccggga atagggctgg aaattgaagg ggcagtgcaa
    51241 cacgccccgc aacccggacg gcattccata cgttgttctc ttaaggttaa ccaccggatt
    51301 gatgagcgcg cacagtacca ccttttttgc accagcaaaa gtgcgaatac cacttgcccg
    51361 aaaggcccgt cgcgagtact ttgtcgcgat atttttgaca ttttcgacta caggaatttt
    51421 tcgatgccaa gagcgaacga aattaaaaaa ggtatggtac tgaattacaa cggcaaactg
    51481 ctgctggtta aggatattga tattcagtcg cccactgccc gcggtgccgc tacgctgtac
    51541 aaaatgcgtt tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt caaaggtgat
    51601 gatatcgttg acaccgtgac gctgacccgc cgttacgttg acttctccta tgtcgatggc
    51661 aacgaatatg tctttatgga taaagaagac tataccccgt ataccttcac caaagatcag
    51721 attgaagaag agttgctgtt tatgccagag ggcggcatgc cggacatgca ggtgctgacc
    51781 tgggacggtc aactgctggc gcttgagctg ccgcagaccg ttgatctgga aatcgttgaa
    51841 accgcaccag gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc gacattgagc
    51901 actggtctgg tgattcaggt accagaatac ttaagcccgg gcgaaaaaat tcgtatccat
    51961 atcgaagaac gccgttatat ggggcgtgct gactaacttc agccgcatgc agaaaaggga
    52021 tagctcaggc tgtccctttt ttaatttatt ataccaatct tctattttgc gcttcctgaa
    52081 caccttacgc cctcaatttt cactcgttga ttgatcgccc tcacacttca tcgcattaac
    52141 aatccagacc aatttcaatt gctgtcatat aactttacac tgtcattgtt aatttatcgt
    52201 tactaagacg tgactcctat gaagacaatt gcctccgtta cgctcccgca tcatgtacac
    52261 gctccacgct acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg ctttggcgcc
    52321 tttcaccgcg ctcatcaggc gttactgacc gatcgtgtgc tgaatgccca gggcggcgac
    52381 tgggggatct gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca gctccgcgca
    52441 cagaaccatt tatataccgt gctggagaaa ggtgcggacg gcaatcaggt gataattgtc
    52501 ggtgccgttc acgaatgcct taatgcaaaa ctggattcct tagcggcaat tattgagaaa
    52561 ttttgcgagc cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg ctattgtatt
    52621 gacccggcca ccggtgcact cgacaccagt aatccgcgga ttattcacga tctacaaacc
    52681 cctgaagaac ctcactccgc accgggtatt ctcgtcgaag cactgaaacg ccgccgtgag
    52741 cgcggcctta caccgtttac cgtgctctcc tgcgacaata ttcccgacaa tggccatgtg
    52801 gtgaaaaacg cggtgctggg aatggcagaa aaatgttcgc cagaactcgc cgggtggata
    52861 aaaaaacacg tcagttttcc gggaaccatg gtcgaccgca ttgttccggc tgcaaccgac
    52921 gaatcactgg cagaaatcag ccagcatctg ggggtaaatg atccctgcgc gattagctgc
    52981 gaaccgttta tccagtgggt ggttgaagat aacttcatcg ctgggcgtcc tgcctgggaa
    53041 gtcgcaggtg tacaaatggt gaatgatgtc ctgccatggg aagagatgaa actgcgtatg
    53101 cttaatggca gccactcttt tctcgcttat ctgggttacc tctcaggatt cgtccatatc
    53161 agtgattgca tgcaggatcg cgtatttcgc catgctgcca gaacattaat gctggatgag
    53221 caagcgccga cactgcaaat taaagatgtc gatttaacac aatatgcgga taagttaatt
    53281 gcacgttttg ctaatccgtc gctgaaacat aagacctggc aaatcgcgat ggatggcagc
    53341 cagaaattac cgcaacgtat gctggcaggt attcgcatac atctggggcg tgaaacggac
    53401 tggtcgttgc tggcattagg cgttgctggc tggatgcgtt acgtcagcgg cgttgatgat
    53461 gccggaaatg ccattgatgt tcgcgatccg cttagcgata aaattcgcga acttgttgcg
    53521 ggcagcagca gtgaacaacg cgtaaccgcc ctgctttccc tgcgtgaagt tttcggtgat
    53581 gatctgccag ataacccgca ttttgtgcag gccatcgaac aagcctggca acaaatcgta
    53641 caattcggcg cacatcaggc gctattaaac accctcaaaa tttaacgatt tctgcggtta
    53701 aagcggatga agctcacctt cgtccgctct ccccttctct tttctgcctt ttttagccag
    53761 gattaacgct cagttaactt accagagtat tgcggagccg ttgtgaccag gaccaacctc
    53821 atcaccggtt ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt agcccataaa
    53881 gatcccaacg aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg aattgatggt
    53941 gctttgctcg ccgatagcgg cgcattgctg aaagagatcc ccggcggctg catgtgctgc
    54001 gttaatggtt tacccatgca ggtagggttg aataccttac tgcgtcaggg aaaaccagac
    54061 cgcttgttga tagagccgac cgggctgggc catccgaaac agatcctcga tctgttaacc
    54121 gcaccagtct atgaactgtg gatagatctg cgcgccacct tgtgcattct cgatccacgc
    54181 ctgctgctgg acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct ggctgccgca
    54241 gacatcattg tcgccaataa atccgaccgt gcgacgcccg aaagtgagca agcgctacag
    54301 cgttggtggc agcaaaatgg tggcgatcga caattaattc acagtgagca tgggaaagtt
    54361 gacggtcatc ttctggattt gccgcgtcgc aatttagccg agttgcccgc cagcgccgcg
    54421 cattctcatc agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa
    54481 cgctggcgtc gcagtctgaa cagcgggcaa ggatatcagg cctgcggctg gatattcgac
    54541 gctgatacgg tattcgacac cattggcatt ctggaatggg cgcgacttgc accggtggaa
    54601 cgcgtcaaag gcgtgctgcg tattcccgaa gggctggtac gaatcaaccg tcagggcgat
    54661 gacctgcaca ttgaaacgca aaacgttgcg ccaccggaca gccgtattga gctgatttcc
    54721 agcagcgaag ctgactggaa tgctttgcag agcgcgctgt tgaagcttcg tttagcgact
    54781 accgcgtaag gttgcctgcg tttttcagta agataattag agaaaatatg attaaaaatt
    54841 tgccgcaaat agtgttgttg aatattgtcg gcctcgcgct gtttctttcc tggtatattc
    54901 ccgttaatca tggattctgg ttgccgattg atgcggatat tttttatttc tttaatcaga
    54961 aactggtcga aagtaaggcc tttttgtggc tggttgcatt gaccaacaat cgcgctttcg
    55021 acggttgttc tctgctggcg atgggaatgt tgatgctgag tttctggctg aaagaaaacg
    55081 cccctggcag acgacgtatc gtgattatgg gtctggtcat gctattaact gcagtggtat
    55141 taaaccagct gggtcaggca ttaattcctg taaaacgggc cagcccaaca ttgactttta
    55201 ccgatattaa ccgcgtcagc gaactgctct ctgttcccac gaaagatgcc tcacgagata
    55261 gctttcccgg cgatcacggc atgatgctgc ttattttttc ggcattcatg tggcgttatt
    55321 tcggcaaagt tgcaggcctt atcgccctta ttatttttgt ggtttttgca tttcccagag
    55381 taatgattgg cgcacactgg tttactgaca tcattgtcgg ttcgatgacc gtgatattga
    55441 tcggtttgcc ctgggtgttg ctgacgccat taagtgatcg attaatcacc ttttttgaca
    55501 aatcactacc gggaaaaaac aaacatttcc aaaacaaata actcacagta attaacatca
    55561 tcaggcttat ttttatagtg aggataatcc tgatgatgcg caccgtgctt tcatccatcg
    55621 cgcgcaaaaa tcattctcta agtaaatgaa tggattgcat gcgtttcact caatcgtact
    55681 ttaattgacc aatcccgctt attaactttc tgtatcactt tttcttataa aaaatcatgt
    55741 aaaaccgctc gccaagaccg caccaatcgg gtaatctaga actcgttttg cctcggcggt
    55801 agattatcct cacagcatat aattttgtgc gttagtccac agatttggcc ttaaggaatt
    55861 gtttcaacat gcccaggtaa ttagtctcgt gtcgcttggc atttttttat aacgatattt
    55921 gtcgttaagg acttcaaggg aaaacaaaca acatggtcaa atctcaaccg attttgagat
    55981 atatcttgcg cgggatcccc gcgattgcag tagcggttct gctttctgca tgtagtgcaa
    56041 ataacaccgc aaagaatatg catcctgaga cacgtgcagt gggtagtgaa acatcatcac
    56101 tgcaagcttc tcaggatgaa tttgaaaacc tggttcgtaa tgtcgacgta aaatcgcgaa
    56161 ttatggatca gtatgctgac tggaaaggcg tacgttatcg tctgggcggc agcactaaaa
    56221 aaggtatcga ttgttctggt tttgtacaac gtacattccg tgagcaattt ggcttagaac
    56281 ttccgcgttc gacttacgaa cagcaggaaa tgggtaaatc tgtttcccgc agtaatttgc
    56341 gtacgggtga tttagttctg ttccgtgccg gttcaacggg acgccatgtc ggtatttata
    56401 tcggcaacaa tcagtttgtc catgcttcca ccagcagtgg tgttattatt tccagcatga
    56461 atgaaccgta ctggaagaag cgttacaacg aagcacgccg ggttctcagc cgcagctaat
    56521 aaaccgtttg gatgcaatcc cttggctatc ctgacgagtt aactgaaagc actgcttagg
    56581 cagtgctttt ttgttttcat tcatcagaga aaatgatgtt tccgcgtctt gatccaggct
    56641 atagtccggt cattgttatc ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca
    56701 tgttcatacg cgctcccaat tctggacgta agctcctgct tacctgcatt gtagcaggcg
    56761 tgatgattgc gatactggtt agctgccttc agtttttagt ggcctggcat aagcacgaag
    56821 tcaaatacga cacactgatt atcgacgtac aaaagtatct cgatacctat tttgccgacc
    56881 tgaaatccac tactgaccgg cttcagccgc tgaccttaga tacctgccag caggctaacc
    56941 ccgaactgac cgctcgcgcg gcgtttagca tgaatgtccg tacgtttgtg ctggtgaaag
    57001 ataaaaaaac attctgttca tctgcgactg gtgagatgga cattccactc aatgaattga
    57061 ttccggcgct caacattaat aaaaacgtcg atatggcgat cttacccggc acgccgatgg
    57121 tgccgaacaa acccgcaatc gtcatctggt atcgcaaccc tttgctgaaa aatagcggtg
    57181 tgtttgccgc tctgaatctc aacctgacgc cttctctctt ttatagttca cggcaggaag
    57241 attacgatgg cgtcgccctc attattggca atactgcgct atctaccttt tcttcacgtt
    57301 tgatgaacgt taatgaatta accgacatgc cggtccgtga aactaaaatt gcgggcattc
    57361 ctctgaccgt tcggctttat gcggatgact ggacatggaa cgatgtgtgg tacgcatttt
    57421 tactgggcgg catgagtgga actgtcgttg gcctgctctg ctattacctg atgagcgtac
    57481 gtatgcgccc cggcagagaa atcatgaccg ccatcaagcg cgaacaattt tacgtggcgt
    57541 atcaaccggt ggtggataca caagctttgc gagtaacggg cctggaagta ctgctacgct
    57601 ggcggcatcc tgtcgcggga gaaattcccc cggatgcctt cattaacttt gccgaatcgc
    57661 aaaagatgat tgtgccgctg actcagcacc tgtttgagtt aattgcccgc gatgccgcag
    57721 aattagaaaa agtgctgcca gtaggcgtca aatttggtat taacattgcg ccggaccatc
    57781 tgcacagcga aagctttaaa gcagatatcc agaaactgct cacttccctg cccgcacacc
    57841 atttccagat tgtgctggaa attaccgagc gcgatatgct gaaagagcaa gaagccacac
    57901 aactcttcgc ctggctgcac tcggtcggcg tagaaattgc tattgatgac ttcggcaccg
    57961 ggcacagcgc gcttatctat cttgagcgtt ttacgctcga ttatctgaaa attgaccgtg
    58021 gatttatcaa cgccatcggt acggaaacga tcacttcacc cgtacttgac gcggtgctga
    58081 cgctggcgaa acgcctcaat atgctgacgg ttgctgaagg ggtcgaaacg ccggaacagg
    58141 cgcgatggct aagcgaacgc ggcgttaatt tcatgcaagg ctactggatt agtcgccctt
    58201 taccgctgga cgattttgtc cgctggctga agaaaccgta tacgccgcag tggtaaggtg
    58261 tgcttacgtc ccttattatt catagtgaaa gcatgccgga ttgcggctaa tgatgagtaa
    58321 aaggaaatcc attgcagatg attgtgcgca tactgctgct gtttatcgct ctgttcacct
    58381 ttggtgtgca ggcgcaggct atcaaggaaa gctatgcctt tgccgtgctg ggcgaacccc
    58441 ggtacgcgtt taatttcaac cattttgatt atgtgaaccc cgccgcgcca aaaggtgggc
    58501 agataacgtt gtcagccctc ggcaccttcg ataatttcaa ccgctatgca ctgcgcggca
    58561 acccgggcgc acgcaccgag cagctgtacg acacgctatt tacgacttcc gatgacgaac
    58621 caggcagtta ttacccgctg attgctgaaa gcgcacgcta tgctgacgat tattcctggg
    58681 tggaggtcgc tattaatccg cgcgcccgtt ttcatgatgg ttcgcccatt actgcccgcg
    58741 atgtagagtt tacttttcaa aaatttatga ccgaaggcgt gccgcaattt cgtctggtct
    58801 acaaaggcac caccgtcaaa gccattgcac cgttaaccgt gcgcattgag ttagctaaac
    58861 ccagcaaaga agatatgctg agtctgtttt cgctgccgat atttccagaa aagtactgga
    58921 aggatcacaa acttagcgac ccgctcgcca cgcctccgct tgccagtggt ccgtaccgcg
    58981 ttacgtcctg gaaaatgggg caaaatattg tctattcccg cgtgaaagat tactgggcag
    59041 caaacttacc ggtaaaccgt ggacgctgga atttcgacac cattcgctac gattattacc
    59101 tcgatgataa tgtcgccttt gaagcgttta aagcaggtgc ctttgatttg cgtatggaaa
    59161 acgacgctaa aaactgggcc acgcgttata ccggtaaaaa tttcgataaa aaatacatca
    59221 tcaaagatga gcaaaagaac gaatcagccc aggatacgcg ctggctggcg tttaatatcc
    59281 aacgtccggt attcagcgat cgccgggtcc gggaagcaat cactctcgcc tttgactttg
    59341 aatggatgaa caaggcgttg ttttacaacg cctggagtcg cacgaacagt tattttcaga
    59401 ataccgaata cgcggccaga aattaccccg acgccgcgga gctggtgctt ctggcaccaa
    59461 tgaaaaaaga tctaccgcca gaagtcttca cacaaatcta ccagccgccg gtatctaaag
    59521 gcgatggcta cgatcgtgac aacctgttaa aagccgacaa acttctcaac gaaacgggct
    59581 gggtgctgaa gggtcagcaa cgcgttaatg ctacaacggg tcagccgctc agctttgaat
    59641 tattgcttcc cgcaagcagc aatagtcagt gggtattgcc gttccagcac agcctgcaac
    59701 ggctgggtat caatatggac attcgcaagg tggataactc gcaaatcact aaccgcatgc
    59761 gcagtcgcga ctatgacatg atgccgcgcc tatggcgggc gatgccgtgg cccagttccg
    59821 atttacagat ttcctggtca tcggaatata tcaattccac ttataatgcc cccggcgtgc
    59881 aaagcccggt tatcgactcg ctgatcaacc aaattattgc cgcgcaggga aataaagaaa
    59941 aattactgcc gttggggcga gcactggatc gcgtattaac gtggaattat tacatgctgc
    60001 caatgtggta catggcggaa gaccgtctcg cctggtggga taaattctcc caacccgctg
    60061 tacgccctgt ttacagcctg ggtatcgata cctggtggta tgacgttaat aaagcggcca
    60121 aactgccgtc agccagacaa cagggagagt agatgggcgc ttacctgatt cgccgtctgt
    60181 tgctggtgat cccaacgtta tgggctatta tcactatcaa ctttttcatc gtgcaaattg
    60241 cgcctggcgg tccggtcgat caggccatcg ccgccattga gtttggtaat gccggagtat
    60301 tacccggcgc aggcggtgaa ggtgttcgtg ccagccatgc gcaaacgggt gtcggcaata
    60361 tcagcgacag taattaccgt ggcggacgcg gattagaccc agaagtgatc gctgagatca
    60421 ctcatcgcta cggtttcgat aagccgatcc acgaacgtta cttcaaaatg ctctgggact
    60481 acatccgctt tgattttggt gatagcctgt ttcgcagcgc ctcggtgctg acgctgatta
    60541 aagacagtct gccggtttcc atcaccctcg gattgtggag cacgctgatt atctatctgg
    60601 tgtcgattcc gttaggcatt cgcaaagctg tttataatgg gagtcgcttt gacgtctgga
    60661 gtagcgcatt tatcatcatc ggctacgcca ttccggcctt tttgtttgcc atcctgctga
    60721 ttgtcttctt cgcgggcggc agctatttcg acctgttccc tctacgcggc ctggtttccg
    60781 ctaactttga ttcgctgccg tggtatcaga aaatcaccga ttatctgtgg catatcacgc
    60841 taccggtgct ggcgacagtg attggtggct ttgcggcgct gaccatgctg accaaaaact
    60901 cattccttga tgaagtgcgc aagcaatacg tggtgaccgc gcgtgcgaaa ggggtaagtg
    60961 agaaaaacat tctctggaaa catgtgttcc gcaacgccat gctgctggtg attgccggtt
    61021 ttccggcgac gtttatcagc atgtttttta ccggctcgct gctgattgag gtgatgtttt
    61081 cactcaatgg tcttggctta ctgggctacg aagcgaccgt ctcgcgcgat tatcctgtaa
    61141 tgtttggtac cttgtatatt ttcaccctga ttggcctgct gctgaatatt gtcagtgata
    61201 tcagctatac gctggttgat ccgcgtatag attttgaggg acgttaatgt cgcgactcag
    61261 ccccgtcaat caggcccgtt gggcgcgttt tcgtcataac cgtcgcggct actggtcgtt
    61321 atggattttc ctcgttttgt ttggtttgag tttgtgttct gaacttatcg ctaacgataa
    61381 accgttgctg gtgcgttatg acggcagttg gtatttcccg ttgttgaaaa actacagcga
    61441 aagcgatttt ggcggcccgc tggcaagtca ggctgattat caggacccgt ggctgaaaca
    61501 acggctggaa aataacggct gggtactgtg ggcaccgatt cgctttggtg ctaccagtat
    61561 caactttgct accgataagc ccttcccttc tccaccctcc cggcaaaact ggctgggaac
    61621 ggatgccaac ggcggcgatg tgctggcacg cattctctat ggcacgcgga tctcggttct
    61681 gtttggcctg atgctgactc tctgttccag cgtgatgggc gtgctggcgg gggcgctaca
    61741 aggctattac ggcggtaaag tcgatctctg gggacaacgc tttattgaag tatggtcggg
    61801 gatgccgacg ctgtttttga ttattttact ttccagcgtc gtacagccta acttctggtg
    61861 gctgctggca attactgtct tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga
    61921 gtttttacgt actcgtaatt tcgactacat tcgtgcggca caggcgcttg gcgtcagcga
    61981 tcgcagtatc atcctgcgtc atatgttgcc taatgccatg gtcgcgaccc tcaccttttt
    62041 accgtttatt ttatgtagtt cgataaccac cctgacctcg ctcgatttcc tcggcttcgg
    62101 tctaccgctc ggttcaccgt cactcggtga actgctgtta caagggaaaa ataaccttca
    62161 ggccccgtgg cttgggatca ccgccttctt gtcggtggcg atattattgt ctttgctgat
    62221 ctttattggt gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc
    62281 aaactctgtt agcgattgaa aatttgtcgg tgggttttcg ccatcagcaa accgtacgta
    62341 cagtagtcaa tgatgtttca ctacagattg aggctggcga aacgctggcg ctggtgggtg
    62401 agtcaggttc aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc
    62461 cggttgaata tctctccggc gatattcgtt ttcatggcga atcgctgctt catgccagcg
    62521 atcaaacgtt gcgcggtgta cgcggtaata agatcgccat gatttttcag gaaccgatgg
    62581 tgtcgttaaa tccattgcat accctggaaa aacagcttta tgaagtgctt tcactccacc
    62641 gcgggatgcg tcgggaagcg gctcgtggcg aaattcttaa ctgccttgat cgcgtcggta
    62701 tccgccaggc ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc
    62761 agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac
    62821 cgaccaccgc actggacgtc tctgtccagg cgcagatttt acagctgttg cgcgaactgc
    62881 aaggcgagct gaatatgggc atgctgttta ttactcataa cctcagcatt gtcagaaaac
    62941 tggcccaccg cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta
    63001 cgctatttgc ctcacccact catccttaca cacaaaagct actcaacagt gaaccgtcag
    63061 gcgatccggt accgttgcca gaacctgcct caacgttgct ggatgttgaa cagcttcagg
    63121 ttgccttccc cattcgcaaa gggattttga agcgcattgt ggatcataat gtggtggtga
    63181 aaaacatcag ttttacgcta cgggcgggtg aaacactggg tttagtgggc gagtctggtt
    63241 ccgggaaaag cacgacggga ctggcgctgc tgcgactgat tacctctcag ggcagcatcg
    63301 tctttgacgg tcagccactg caagatttaa atcgccgcca gctgttacct actcgtcatc
    63361 gtattcaggt ggtatttcag gatccaaact cctcgctcaa cccacgactc aacgttttgc
    63421 agattattga ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac
    63481 aacaagtgat agccgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc
    63541 cggcggagtt ctctggtggt cagcgacaac gtattgcgat tgccagggca ttaattctta
    63601 agccctcact gatcatactt gatgaaccga catcatcact cgacaaaacg gtacaggcgc
    63661 aaatattgac gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca
    63721 gccacgattt gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg
    63781 aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccgc accgcagcag gagtatacgc
    63841 gtcagctact ggcgttgagc tgacgcttaa aaaggattgt agtttgaaaa gggttcggca
    63901 attgccacac caaaattttt caatcggcat gttgcagcaa actcgtcctg gcgatttaca
    63961 aacaggcacg gctcaccttc acactccagc accgaacatg gaacttcgat gtcgcttaac
    64021 gcctggctta gtttatacat caccgaccat gcattatcac catccgggct aagcaattta
    64081 agcgccacca ggctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa
    64141 cctgcgaaac cagcagacca gcgaaagttc tgcggtagac gatggacgat tgagagttgt
    64201 aatgaagtca cttattttcc ccggaagcac atttacttca caatttattt acatcaattt
    64261 taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgcctgcat
    64321 ggcctctatg gctctgattt aagtaatatc tgcgggcgtg gtcacgtttg cggggctata
    64381 tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat
    64441 aaattgattt tacataaaat aaacatatat cgggggaatg atagatttgt ggttgacatg
    64501 catcaacaaa ggaagccttt tagcttcctc gttgtgcaat agatcaccgt tttttcggtc
    64561 gactggcgta cagacagaag agaatggagc tggttgcgca gaatgcaatt gaccaaatca
    64621 tcggccatgc agagttaaag gtcgcaagag aaagcaatgc gccaacaatt gcccctatgc
    64681 caaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa
    64741 gaatgaccgc catcgcattg gatgacacca tagacacgca gcccacaaac gccgcaacgc
    64801 caaccaccag cgaccagaat cccagcccca gcagcgcact gatgaccatc cacgctgcca
    64861 taataaattg tatccacaac cccgagcgga acatatttaa cgcgccaatg cggcggacga
    64921 agcggctgtt aaagatggtc atcacgaaca gaaaaacaat gtttagcgca aagtaataac
    64981 caaagttttc cggtgcaacg tggttgattt caatgtagac aaacggtccg gcgctcagga
    65041 acgagaacat tccggcaaag ctgaaaccgc tggccagcat ataactcagg acacgcttat
    65101 gacggaacag cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc tggcgaggat
    65161 ccagaagatg taatgccagc tcagccacac cagcacccag ccgccaacta tcggtgccat
    65221 cagcggtgca atggtcgtca ccagcatgac aaacgacatc atccgcgaga actcttcttt
    65281 cgggtaaata tcacgcatca aagcgttgat aaccacgctg gccgcagccg cagccagccc
    65341 gtggaagaaa cgcatcacaa tcagctgatc gatggtttgt gccaacgcac acgccaccgc
    65401 ggcggcggca aacaccagcg taccgccaag aaccaccggc ttacgcccga agctgtctgc
    65461 catcggcccg tagattaact gccccaacgc aaagcccaga atataagtac tcagggtcat
    65521 ctgcgtactg cccgccggta cgccaaactg cgctgaaatt accggtagcg cgggcagata
    65581 catatcaatc gacagcggca tcaacatggc cagcaggcca aggataaaaa caatagcaaa
    65641 cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa gttagacgac
    65701 gctggcaatt tcttcttcag ttaacggacg atattcaccg ggggctaaat cagcatccag
    65761 cgtaatgccg ccaatacgtt cacgatgcag ctcaaccaca tggttaccca cggcggcgaa
    65821 catgcgtttc acctgatgat aacgcccttc gctgatggtc agacgaacct gcgttggggt
    65881 aatcacttcc agcaccgccg gcttagtgag atctttttcg ttatgcagct gcacgccttt
    65941 agcaaattgt tctgccgtat cgtcagctac gggtgatacc agcgtcacca gataggtctt
    66001 ctcgcaatga tggcgcggag aagtaatgcg gtgcgaccac tgaccatcat ctgtcattag
    66061 taccagaccg gtggtatcga tatccagccg ccctgcggcg tgcagtttcc acgctaccgg
    66121 ttcatctaga aaatagagca ccgttgggtg atcagggtcg tccgtggagc aaacataacc
    66181 ctgaggctta ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc
    66241 gtaagcgaca tcatgttcag gaagcagttt gaacgctgca ttacggacga tttcgccatc
    66301 gacggtgaca cgattgccgc ggatttcacg cccggcaata gcacggctaa cgccgagttg
    66361 ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa acggaggtcg
    66421 ggtaatgacc cgaatatctg aactgttgtc tgcccagtat agcggtctaa taacatccct
    66481 caagggaaaa agatccgtgg catactatta gcagaataat ctacctacgc gagaccatga
    66541 tttttacact tcgcccatat cagcaagaag ccgtggatgc cacgctcaac cattttcgtc
    66601 gtcataaaac ccctgccgtt atcgtgctgc ccaccggtgc aggtaaaagc ctggtgatag
    66661 cggaactggc acggctggct cgtggtcgcg tactggtgct ggcacacgtt aaagaactgg
    66721 tggcgcaaaa ccatgcaaag tatcaggcgc tggggctgga agccgatatt tttgccgccg
    66781 ggctgaagcg taaagagagc cacggtaaag tggtatttgg cagtgtgcag tcggtcgccc
    66841 gtaatattga tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa tgtcaccgta
    66901 ttggtgacga tgaagagagc cagtatcagc aaatcctcac tcacctgaca aaagtgaatc
    66961 cccacttacg cctgctgggg ctgactgcca cgccttttcg attgggcaaa ggttggatct
    67021 accagttcca ttatcacggc atggtacgca gcgatgagaa agcccttttc cgtgactgca
    67081 tttatgagct gcccctgcgt tatatgatta aacacagcta tctgacgccg ccagaacgac
    67141 tggatatgcc agtagtgcaa tatgatttca gccgcttgca ggcacagagc aacgggctgt
    67201 tcagcgaagc cgatctcaac cgtgagctga aaaaacagca acgtattacc ccgcacatca
    67261 tcagccagat tatggagttc gccgaaaagc gtaaaggagt gatgattttc gccgcgacgg
    67321 tcgaacacgc aaaagagatt gtgggattac tgcctgccga agatgcagca ctgattactg
    67381 gcgacacccc cggcgctgag cgcgatgtgt taattgaaaa ttttaaagcc cagcgttttc
    67441 gctatctggt caacgtcgcg gtactgacca ccggatttga cgccccgcac gtcgatctta
    67501 tcgccattct gcgccctacc gaatcagtga gtctttacca acaaattgtc gggcgcggtc
    67561 tgcgtctcgc tccgggcaag actgattgtt taattcttga ttatgcgggt aatcctcacg
    67621 atctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg
    67681 ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc gccgacggga
    67741 cattgattga acactttggt cgccgctgtc agggatggtt tgaagatgac gacggtcatc
    67801 gcgaacagtg tgacttccgt ttccgtttta aaaattgccc gcaatgtaac gcagaaaatg
    67861 atattgccgc tcgccgctgc cgcgaatgtg acacggtact ggttgatccg gatgatatgt
    67921 taaaagcggc gctacgactg aaagatgcgc tggtattacg ctgtagcggc atgtctttgc
    67981 agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat gaagacggtg
    68041 cggatgtgag tgagcgtttc cgtctgcaaa cacctgccca gcgtaccgcc ttcgagcagc
    68101 tttttatccg cccgcatacg cgcacaccgg gcatcccgct gcgctggatc accgccgccg
    68161 atatcctcgc ccagcaagcc ttattgcgac ccccggattt tgtcgtcgcc cgcatgaaag
    68221 gccagtactg gcaggtgcgt gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc
    68281 acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag tatagaatct
    68341 cacccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc ctgtagcagg
    68401 attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa agagcagggt
    68461 aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat ctacggtggc
    68521 aaagaagcgc cgctggctat cgagctggat cacgacaaag tcatgaacat gcaagctaaa
    68581 gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat caaagttaaa
    68641 gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc agcacatcga cttcgttcgc
    68701 gcttaattgc tgaataagtt gtaaaaaacc ccgctcccgc ggggtttttt gtatctgcag
    68761 attatgcctg attacggtat tgctattttt tgcaggccag ataaggcgtt cacgccgcat
    68821 ctggcattca gaacacaaaa ccgattaatt gccgccagac gtccggcgct gcaattggtc
    68881 gcgcaaattc ggcggcgtgc ctttaatggt cagggtatcg gtcgccggct cccagaagat
    68941 gcgttcacct aacagcatcg catcaaagtt aatagtcaag ccaccgccgc taccggcaaa
    69001 cttcgtcaac tgacgcagtg tgctccggtc tgccgggaag ctctcttcca gttcgtagcc
    69061 tttttcagcc gcaaactccg tgaagctcac ttcgcttacg cctgccagct ctttcgacag
    69121 cgattccagt tcgatctctt cgcctgcctg cagctgctca ttacagtagc tataaacctg
    69181 ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga aatcatcaac
    69241 cgcttgcaac agcccacggt tttgtgcttt cgtgttcaat ccttcgctgg ccccgaggaa
    69301 atccataaag aaatccgcca ctttgcgccc tactcgccct ttcaggaaag tgagatagcg
    69361 ggtggactct ggattggttt cccattcggt taaatcgata cgcgcaacaa tatccgcatg
    69421 attgatatca agataatggg ttgggttgat gtcgagattt tcgttaacac gcatactgct
    69481 caggttgctc agaactgcca ccagcagata ctccaccgcc agataacggt agtggcaaaa
    69541 cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca ggcgaccagt
    69601 tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc gcagcgtctg
    69661 cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc tatagacccg
    69721 atgcagttct gccaccatct caacgacggt ttctgtcggt tccagcaatg aatcgcgcaa
    69781 caccagctca aggttctgct catcacgctt gataagctgg tgcagagcaa tctggttgat
    69841 atccagactc atgataaact ctcctttaag accgggcggt attcaaccac cgccggttaa
    69901 gcgacgcaat aaaagatggc ggtcatttta cagaaggcag cgtaacgaga atgatttaac
    69961 gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt catcaactaa
    70021 ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg aacaattgct
    70081 cgcagagctg ctcaacgtac tggaaaaaca taaggctccg accgaccttt ccctgatggt
    70141 gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc aacgccaggc
    70201 aattgccaac tcttttgccc gcgccttaca gtcctctatc aacgaagaca aagcgcacta
    70261 agggaaacag ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc
    70321 agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg ctcgtcattg
    70381 gcagccgtta cctgtttatc gccgactggc cgacaacgct tgctggtcgc atttattcct
    70441 acgtaagcat tatcggccat ttcagcttcc tggtgttcgc cacctacttg ctgatcctct
    70501 tcccgctgac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc gtcattctgg
    70561 caacggcggg aatgacgcta ttactgatcg atagcgaagt ctttactcgt ttccatctcc
    70621 atcttaatcc catcgtctgg cagttggtta tcaacccaga cgaaaatgag atggcgcgcg
    70681 actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaacta gtgtttgcga
    70741 cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg acgcttcgcg cgcccgctgg
    70801 ccgcattctt atttatcgcc tttatcgcct cgcatgtggt gtatatctgg gccgatgcca
    70861 acttctatcg cccgatcacc atgcagcgcg ctaacctgcc gctttcgtac ccgatgacgg
    70921 cgcgacgttt tcttgagaag catggtctgc ttgatgcgca ggagtatcaa cgccgtctta
    70981 ttgagcaagg taatccagac gccgtttccg ttcagtatcc gttaagcgaa ctgcgctatc
    71041 gcgatatggg caccgggcag aatgtgctgt tgattactgt cgatggcctg aactactcac
    71101 gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt tcgttcacgc
    71161 gccatatgag ctccggcaac actacagaca acggcatctt tggcctgttc tatggtatct
    71221 cgccgagcta tatggacggc attctgtcga cccgtacgcc tgcggcatta attactgcgc
    71281 ttaatcagca aggctatcag ctggggttat tctcatcaga tggctttacc agcccgctgt
    71341 atcgccaggc attgttgtca gatttctcga tgccgagcgt acgcacccaa tccgacgagc
    71401 agaccgccac gcagtggatc aactggctgg gccactacgc acaagaagat aaccgctggt
    71461 tctcgtgggt ttctttcaat ggcactaaca ttgacgacag caatcagcag gcatttgcac
    71521 ggaaatatag ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg ctcaatgcac
    71581 tgcgtgattc tggcaaactg gacaatacgg ttgtgattat cactgccggt cggggtattc
    71641 cgctgagcga agacgaagaa acctttgact ggtcccacgg tcatctgcag gtgccattag
    71701 tgattcactg gccaggcacg ccggcgcagc gtattaatgc gctgactgat cataccgatc
    71761 tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc gaatattcgc
    71821 aaggtcagga tttgttcaac cctcaacgcc gtcattactg ggttactgca gcggataacg
    71881 atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac ggtaaatacc
    71941 gcacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta agtttgttat
    72001 tgcaagtgct gacagacgag aagcgtttta tcgctaactg attaattata aatcagttag
    72061 cgaaatatct tacttgcaat cggtgtggaa aacggtagta ttagcagcca cgagtcggca
    72121 cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt tcaaatcctc
    72181 tcgtgccgac caaaaatccc aagaaaaaac caacctttac ggttggtttt tttatatctg
    72241 caattaattt gataaacaga ccgtgacaca tcacagcctg tttattttct gttatcagaa
    72301 cgtccagacc actcccgcct gagtattcca cggcgattct acccctgcgc cattgccata
    72361 gctgactgac aaatgaccgc ttaacgtcgg ggtaaacgat gaccttatcc cagcctgata
    72421 aacgccacgg gttccagcca catcattatt aaatttgcca tcgtcattaa ctttcacatg
    72481 gttagaatcg gcgtattcct gacgcacggc ggctttcagc cagggttcca acgtcgtacc
    72541 gttttgcagg tccatgtgat agcttaccgc cgttcccgct tcagcgcgta atatccgggt
    72601 atttcccaca tccgcgcgca tgccgtttga taacgtgtag tcctgaccat ctgtggtaaa
    72661 gccggtaaag gcgagatagg gtctaacact ccacaatccg tcaacccaac ggaacccgct
    72721 ctcaacatga gcacccgcgc cgttactatt gtaatcgcca aacgctgttg ccccattact
    72781 catcttgcca tggatggtgt tggcaaaacg gtcaactttc accaccccat caacataggc
    72841 accgttctga tgctcccaac cggcataagc ccccagggtg tagctatcaa tattgccttt
    72901 gccgccgcga tcaaaaccaa tatcagaatg agagtaatca aagaacaagc cgcgaattgt
    72961 gctgctttct tcacgggaga aacggctatc gataccgagc gtcaggcccg tcaatgtttg
    73021 ctcaaagcca gctcccgcat cagtggtcac gttgttgcgg gtgttaattg ccgaactcca
    73081 catcgccgta tcgtaattaa cgccttttac gctaccaaga cgctcacgca cggtgtccag
    73141 ttctgcatca aataccagcg gttgtgctgc tgccatgttc agcacatcag tggttgaagg
    73201 ggtaatttgc gcgcgattct ctgcgaggct ccagctatgg ttgccattat ccagcaaggt
    73261 atattcatac gtaccgatat caacaacgcc tccggcattg cccaacgtga atgcagcatc
    73321 accgccgccc gtcgttacca gtgtaaggct atctcctgct gccgggctgg cccccgtgtc
    73381 cgtcacgaat attttgaaat cacctgttgc ctgaccgatg acgttgagct gatcgctctg
    73441 atgattagcc atatcggtac gcatataaaa ttgccgtttc ccgacagggt attggtggtc
    73501 aacgtaatga aattttccgc acttgccgtc acagatgaac gagtcactgg ctcagccatc
    73561 ataatcacat ttgcgccgga catcgcaagg ttggctaagt tgaggcgagt gttttggttc
    73621 gtcgtagtga tatcggcaat gatggtccat gcgctatttt cgagcgaaac gtccgcttgg
    73681 ctggtatcca cggcaccatg cgttctaaaa gaagcgccct cagaaaccac taccttaccc
    73741 accaacatag cgggtgcata atttattttc ggcttcatga cctcaagaat gccatcattc
    73801 cctctgactg acacgttaac cattgtgcca gcccagacat tagcgcggcc attgttgata
    73861 accatatttt cagagatggc tttagaggaa tacgtgatac ttccattctg atctgatcga
    73921 ccgagatcat atacagcatt tgcctgcact ttcgtaccag tatcctttcc cagcgattgc
    73981 atagtggcat gctcatcaag gatagtgtca atggcctgag tgtcctccat aacaatgagc
    74041 ccggagccat catccagttc ataatttttt gacacaccat cctttatact aaattggcct
    74101 ttactgtttg taccactcac ttccagcgca ttcgttgaga caattaattt cccgcctgct
    74161 tttttatcaa cgccagttgc agtcccgcca tccataacct ccagtaatcc tcccttttcg
    74221 atagcggcaa agacggcaat cccatctttt tcaacaatta agcgcccgcc tttaatcgtg
    74281 ggatcattcg ccgcg
//
LOCUS       DAJTXQ010000004        70301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000004 DAJTXQ010000000
VERSION     DAJTXQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.004"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..673)
                     /locus_tag="OO515_000237"
     CDS             complement(<1..673)
                     /locus_tag="OO515_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR6574267.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OO515_000238"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="OO515_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6574268.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OO515_000239"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="OO515_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6574269.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OO515_000240"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="OO515_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6574270.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OO515_000241"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="OO515_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6574271.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OO515_000242"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="OO515_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6574272.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OO515_000243"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="OO515_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6574273.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OO515_000244"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="OO515_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6574274.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OO515_000245"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="OO515_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6574275.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="OO515_000246"
     CDS             complement(9727..9837)
                     /locus_tag="OO515_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6574276.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OO515_000247"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="OO515_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6574277.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GALITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OO515_000248"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="OO515_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR6574278.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OO515_000249"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="OO515_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6574279.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OO515_000250"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="OO515_000250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6574280.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="OO515_000251"
     CDS             complement(14170..14742)
                     /locus_tag="OO515_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6574281.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="OO515_000252"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="OO515_000252"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6574282.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="OO515_000253"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="OO515_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6574283.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="OO515_000254"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="OO515_000254"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6574284.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="OO515_000255"
     CDS             17911..18759
                     /locus_tag="OO515_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6574285.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="OO515_000256"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="OO515_000256"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6574286.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="OO515_000257"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="OO515_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6574287.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="OO515_000258"
     CDS             complement(21671..21871)
                     /locus_tag="OO515_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6574288.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="OO515_000259"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="OO515_000259"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6574289.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="OO515_000260"
     CDS             complement(23030..23386)
                     /locus_tag="OO515_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6574290.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="OO515_000261"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="OO515_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6574291.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="OO515_000262"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="OO515_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6574292.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="OO515_000263"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="OO515_000263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6574293.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="OO515_000264"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="OO515_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6574294.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="OO515_000265"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="OO515_000265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6574295.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="OO515_000266"
     CDS             31857..32900
                     /locus_tag="OO515_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6574296.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="OO515_000267"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="OO515_000267"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6574297.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="OO515_000268"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="OO515_000268"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6574298.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="OO515_000269"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="OO515_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6574299.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38830..39165
                     /gene="eutS"
                     /locus_tag="OO515_000270"
     CDS             38830..39165
                     /gene="eutS"
                     /locus_tag="OO515_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6574300.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39178..39657
                     /gene="eutP"
                     /locus_tag="OO515_000271"
     CDS             39178..39657
                     /gene="eutP"
                     /locus_tag="OO515_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6574301.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39632..40333
                     /gene="eutQ"
                     /locus_tag="OO515_000272"
     CDS             39632..40333
                     /gene="eutQ"
                     /locus_tag="OO515_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6574302.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40330..41133
                     /gene="eutT"
                     /locus_tag="OO515_000273"
     CDS             40330..41133
                     /gene="eutT"
                     /locus_tag="OO515_000273"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6574303.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41130..42146
                     /gene="pta"
                     /locus_tag="OO515_000274"
     CDS             41130..42146
                     /gene="pta"
                     /locus_tag="OO515_000274"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6574304.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42185..42478
                     /gene="eutM"
                     /locus_tag="OO515_000275"
     CDS             42185..42478
                     /gene="eutM"
                     /locus_tag="OO515_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6574305.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42585..42872
                     /gene="eutN"
                     /locus_tag="OO515_000276"
     CDS             42585..42872
                     /gene="eutN"
                     /locus_tag="OO515_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6574306.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42884..44287
                     /locus_tag="OO515_000277"
     CDS             42884..44287
                     /locus_tag="OO515_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6574307.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44298..45134
                     /gene="eutJ"
                     /locus_tag="OO515_000278"
     CDS             44298..45134
                     /gene="eutJ"
                     /locus_tag="OO515_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6574308.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45124..46311
                     /gene="eutG"
                     /locus_tag="OO515_000279"
     CDS             45124..46311
                     /gene="eutG"
                     /locus_tag="OO515_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR6574309.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46628..47854
                     /gene="eutH"
                     /locus_tag="OO515_000280"
     CDS             46628..47854
                     /gene="eutH"
                     /locus_tag="OO515_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR6574310.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     CIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47851..49254
                     /gene="eutA"
                     /locus_tag="OO515_000281"
     CDS             47851..49254
                     /gene="eutA"
                     /locus_tag="OO515_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6574311.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49266..50627
                     /gene="eutB"
                     /locus_tag="OO515_000282"
                     /pseudo
     CDS             49266..50627
                     /gene="eutB"
                     /locus_tag="OO515_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50648..51535
                     /gene="eutC"
                     /locus_tag="OO515_000283"
     CDS             50648..51535
                     /gene="eutC"
                     /locus_tag="OO515_000283"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6574312.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51545..52204
                     /gene="eutL"
                     /locus_tag="OO515_000284"
     CDS             51545..52204
                     /gene="eutL"
                     /locus_tag="OO515_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6574313.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52217..52717
                     /gene="eutK"
                     /locus_tag="OO515_000285"
     CDS             52217..52717
                     /gene="eutK"
                     /locus_tag="OO515_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6574314.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52763..53815
                     /gene="eutR"
                     /locus_tag="OO515_000286"
     CDS             52763..53815
                     /gene="eutR"
                     /locus_tag="OO515_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6574315.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="OO515_000287"
     CDS             complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="OO515_000287"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6574316.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="OO515_000288"
     CDS             complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="OO515_000288"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6574317.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55807..56232
                     /locus_tag="OO515_000289"
     CDS             55807..56232
                     /locus_tag="OO515_000289"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6574318.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56219..56668
                     /locus_tag="OO515_000290"
     CDS             56219..56668
                     /locus_tag="OO515_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6574319.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56729..57304
                     /locus_tag="OO515_000291"
     CDS             56729..57304
                     /locus_tag="OO515_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6574320.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57400..58299
                     /gene="yfeX"
                     /locus_tag="OO515_000292"
     CDS             57400..58299
                     /gene="yfeX"
                     /locus_tag="OO515_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6574321.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="OO515_000293"
     CDS             complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="OO515_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR6574322.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59666..59839)
                     /locus_tag="OO515_000294"
     CDS             complement(59666..59839)
                     /locus_tag="OO515_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR6574323.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59852..61090)
                     /gene="murP"
                     /locus_tag="OO515_000295"
                     /pseudo
     CDS             complement(59852..61090)
                     /gene="murP"
                     /locus_tag="OO515_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="OO515_000296"
     CDS             complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="OO515_000296"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6574324.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62154..63011
                     /gene="murR"
                     /locus_tag="OO515_000297"
     CDS             62154..63011
                     /gene="murR"
                     /locus_tag="OO515_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6574325.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63140..63931
                     /gene="ucpA"
                     /locus_tag="OO515_000298"
     CDS             63140..63931
                     /gene="ucpA"
                     /locus_tag="OO515_000298"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6574326.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64102..65118
                     /gene="cysP"
                     /locus_tag="OO515_000299"
     CDS             64102..65118
                     /gene="cysP"
                     /locus_tag="OO515_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6574327.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65118..65951
                     /gene="cysT"
                     /locus_tag="OO515_000300"
     CDS             65118..65951
                     /gene="cysT"
                     /locus_tag="OO515_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6574328.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65951..66826
                     /gene="cysW"
                     /locus_tag="OO515_000301"
     CDS             65951..66826
                     /gene="cysW"
                     /locus_tag="OO515_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6574329.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66816..67913
                     /gene="cysA"
                     /locus_tag="OO515_000302"
     CDS             66816..67913
                     /gene="cysA"
                     /locus_tag="OO515_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6574330.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68047..68958
                     /gene="cysM"
                     /locus_tag="OO515_000303"
     CDS             68047..68958
                     /gene="cysM"
                     /locus_tag="OO515_000303"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6574331.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68961..69329)
                     /locus_tag="OO515_000304"
     CDS             complement(68961..69329)
                     /locus_tag="OO515_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6574332.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69434..70285
                     /gene="pdxK"
                     /locus_tag="OO515_000305"
                     /gene_synonym="thiJ"
     CDS             69434..70285
                     /gene="pdxK"
                     /locus_tag="OO515_000305"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6574333.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16699 a        18349 c        19436 g        15817 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tggccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga gcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggttgtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa agtcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaagc
    38641 agtctatata atatctcgat attattttat ttatattcat gcgttgcata tgaaagttta
    38701 tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta aacgcgcttg
    38761 ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt ttttcacagg
    38821 cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa acaggtcaca
    38881 ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg cgttccggat
    38941 gcgggcgtaa ttggcattat gacgctaact cccggcgaaa cggcgatgat cgtcggtgat
    39001 ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag cggtgcattg
    39061 gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt cagcggttta
    39121 ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga ggcattcatg
    39181 aaacgaattg cgtttgtggg tagcgtcggg gcaggaaaaa caacgttatt taatgcgttg
    39241 caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga taagggcgat
    39301 attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt aattaccacg
    39361 ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga aagtcggttg
    39421 cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat cagtaaaacg
    39481 gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac gggtttcgaa
    39541 gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact ggtggattat
    39601 ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag cgaatgatat
    39661 tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg ccagcattat
    39721 taccccggaa gcccgtaaag tggcggatct actgggtttt accatcaccg aatgtgatga
    39781 atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa ccgaaagcca
    39841 gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg aaagcctggt
    39901 cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg gcgcaatgca
    39961 gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg gcagcagcgt
    40021 caagtttggt cgttttgatg gcgcacaacc gcattgcgtg ggcttgactg atcttgtgac
    40081 cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg cattcttccc
    40141 gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggagctcc atgttcgcca
    40201 cgaaggcgaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga aaggctccag
    40261 catcgaattt ggtacgacat ccagcgtgaa attcctgtat gtcgcctggc cggctaactg
    40321 gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca tacgctcagc
    40381 gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc ccgggagttg
    40441 ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct gtttgttgac
    40501 gatgaacagc agcagccgca gcccgttcat gggctgacca gtagcgatga acatccgcag
    40561 gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct gactcacctg
    40621 tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc ggttctcgac
    40681 agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg gcagccgtgg
    40741 ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct gggggaaccg
    40801 ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcgact ttctcaccag
    40861 ccgctgcgct atctcgacca cgatcatctg gtgccggaag ccagccacgg gcgcgatgcg
    40921 gcgttgctca atctgctacg taccaaagtg cgggaaaccg aaaccgtagc ggcgcaggtg
    40981 tttatcaccc gcagctttga agtattacgc ccggacatct tgcaggcgct gaaccgcctt
    41041 tcgagcacgg tctacgtaat gatgatcctg agcgtaacga agccgccgct aacggtgaag
    41101 cagatccaac aacgactggg ggaaacgcaa tgattattga acgttgtcgt gaactggcgt
    41161 tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg ctgaaagctg
    41221 cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgctaat ccgtttgaac
    41281 ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg attgatccgc
    41341 atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa
    41401 aaacgccgcc ggatgcgctg gaaaaactta ccgatccgct gatgttcgcc gcagcaatgg
    41461 tcagcgccgg taaagcggat gtctgtatcg cgggcaacct ctcttccacg gcgaatgtgc
    41521 tgcgtgccgg attacgtatt atcggcttgc agccaggctg taaaacgctc tcatccattt
    41581 tcctgatgct gccacagtac agcggtccgg cgttgggctt tgccgattgc agcgtggtgc
    41641 cacagccgac ggcggcgcaa ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg
    41701 ccctcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagtaac ggtagcgccc
    41761 gtcacccctg cgttgccaac gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa
    41821 agctggtggt tgatggcgag ttgcagtttg acgccgcctt cgtgccggaa gtggcggcgc
    41881 aaaaagcgcc tgccagcccg ctacagggca aagccaatgt gatggttttt ccgtcgctgg
    41941 aagccggaaa tattggctac aaaatcgcac aacgactcgg cggatatcgt gccgtcgggc
    42001 cattgataca aggacttgcc gcgccgatgc acgatctctc tcgtggttgt agcgtgcagg
    42061 aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa
    42121 gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata aagaggaaag
    42181 cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga ttgaggcttc
    42241 tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg
    42301 cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg
    42361 tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc cgcgtccgca
    42421 cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca acctgtaatc
    42481 accctgtgtg acgtcgattc gggcgtcacg ctctctcctt tttcatttta ccttctgcgg
    42541 agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg
    42601 tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac aaattgctga
    42661 tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc gccatcgaca
    42721 atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc gcccgccagg
    42781 cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg
    42841 tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc aacaggatat
    42901 tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc
    42961 cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc
    43021 ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc
    43081 aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg gcatgggccg
    43141 cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg
    43201 tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa acgcgccctg
    43261 gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa ttaataacgc
    43321 catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa
    43381 agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga
    43441 aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt
    43501 tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa
    43561 acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag tggtggatga
    43621 aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa
    43681 catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg atgaactgat
    43741 gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc agcagctgca
    43801 accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt
    43861 tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg
    43921 tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc
    43981 ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact
    44041 ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa acatgaacca
    44101 gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgcgca ttgccgggct
    44161 ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg gtgaaggggt
    44221 aactagcgtg cgtacgtttg tccgtctgcg tcgctgtgta ttagtcgatg cgtttcgcat
    44281 tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt ctgcaaacgg
    44341 cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg
    44401 atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag tcggtggcgg
    44461 tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg
    44521 tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc cgttttagcc
    44581 atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac gtgctggagt
    44641 ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg gatctgctgc
    44701 aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt gccatcgtga
    44761 aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac atctctctga
    44821 ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag cgcggtcacg
    44881 gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggtggac attgtcgccc
    44941 ggcatattga agggcagggc attactgatt tatggctggc gggcggctct tgtatgcaac
    45001 cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc
    45061 acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc
    45121 tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa
    45181 cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc ggtgagcagt
    45241 tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc agacagcttt
    45301 ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc
    45361 atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg
    45421 gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg
    45481 gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct ggcagagatg
    45541 tcagaaacca gcgttctgca accgcgcttg ccgctgattg ccattccaac gaccgccgga
    45601 accggctctg aaaccaccaa tgtaacggtg attatcgacg cggtgagcgg gcgcaagcag
    45661 gtgttagccc atgcctcgct gatgccggat gtggcgattc tcgacgccgc gttgaccgaa
    45721 ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg cgttaaccca tgccattgaa
    45781 gcatacagcg ccctgaacgc tacaccgttt accgacagtc tggcgattgg tgccattgcg
    45841 atgattggca aatcgctgcc gaaagcggtg ggctacggtc acgaccttgc cgcgcgcgag
    45901 agcatgttgc tggcttcatg tatggcggga atggcgtttt ccagtgcggg tcttgggttg
    45961 tgccacgcga tggcgcatca gccgggcgcg gcgctgcata ttccacacgg tctggcgaac
    46021 gccatgttgc tgccaacggt gatggaattt aaccggatgg tttgtcgtga acgctttagt
    46081 cagattggtc gggcactgcg aactaaaaaa tccgacgatc gtgacgctat taacgcggta
    46141 agtgagctga ttgcggaagt tgggattggt aaacgactgg gcgatgttgg tgcgacatct
    46201 gcgcattacg gcgcatgggc gcaggccgcg ctggaagata tttgtctgcg cagtaacccg
    46261 cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg cggcgcaata aatgccggat
    46321 gcgacgcttg ccgcgtctta tccagcctac gggattgcac atgtagggcg gataaggcgt
    46381 tcacgccgca tccgccagtg gcgcggtgca attgccggat gcgatgcttg ccgcgtctta
    46441 tccggcctac gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    46501 gcgcggtgca attgccggat gcgacgcttg ccgcgtctga tccggccttc gggattgcac
    46561 atgtagggcg gataaggcgt ttacgccgca tccgccaata aataacaact aacagggagt
    46621 aaaggcgatg ggaattaacg aaatcatcat gtacatcatg atgttcttta tgctgatagc
    46681 tgccgtagac aggatcctgt cgcagttcgg cggttctgct cgtttcctcg gtaagttcgg
    46741 taaaagtatc gaaggatcag gcggtcagtt cgaagaaggc tttatggcaa tgggcgctct
    46801 gggtctggcg atggtcggta tgaccgcgct ggcaccggta ctggctcacg tactcgggcc
    46861 ggtaattatc ccggtttacg aaatgctcgg cgcaaacccg tcaatgttcg ccggaacgct
    46921 gctggcgtgc gatatgggcg gcttcttcct cgccaaagag ctggcgggcg gcgacgtagc
    46981 agcgtggcta tactctgggt taattcttgg gtcgatgatg gggccaacga ttgtgttttc
    47041 cattccggtg gcgctcggca ttatcgaacc ttctgaccgt cgttatctgg cgctcggcgt
    47101 gctggcgtgc attgtgacca ttccgattgg ctgtattgct ggtggtctgg ttgctatgta
    47161 ctccggtgtg cagatcaacg gtcagccggt ggaattcact ttcgccctga tcctgatgaa
    47221 catgatcccg gtgatcattg ttgcgattct ggtggcgctg gggctgaaat tcatcccgga
    47281 aaaaatgatc aacggcttcc agatcttcgc caaattcctc gttgcattga tcaccctcgg
    47341 tcttgctgcc gcggtagtta agtttctgct tggctgggaa ctgatccccg gtctggaccc
    47401 tatctttatg gcccctggcg ataaacccgg tgaagtgatg cgcgccattg aagttatcgg
    47461 ttctatctcc tgcgttctgt taggggcgta tccaatggtg ctgctgctga cccgctggtt
    47521 tgaaaaaccg ctgatgagcg tcggtaaagt gctgaatatg aacaacatcg cggcagccgg
    47581 catggtggca acgcttgcca acaacatccc gatgttcggc atgatgaagc agatggatac
    47641 ccgcggcaaa gtcatcaact gcgccttcgc cgtttccgct gctttcgccc tgggcgacca
    47701 cttaggcttc gccgctgcca acatgaacgc catgatcttc ccgatgattg tcggcaagtt
    47761 gatcggcggc gtaacggcga ttggcgtggc gatgatgctg gtgccaaaag aagacgcgac
    47821 cacggctaaa accgaagcgg aggcacaatc gtgaacactc gccagctatt gagcgtcggt
    47881 atcgatatcg gcaccaccac cacccaggtg attttctccc acctggagct ggttaaccgt
    47941 gcggcggtgt cgcaggtgcc gcgctacgaa ttcattaaac gcgaaattag ctggcaaagc
    48001 ccggtgttct ttacccctgt cgataaacag ggtggtttaa aagaagcgga actgaaaacc
    48061 ttaatcctcg agcaatatca ggctgcgggc attgcgccgg aaagcgttga ttctggtgcc
    48121 atcatcatca ccggtgaaag cgcgaaaacc cgcaatgctc gtccggcggt gatggtgctc
    48181 tcccagtcgc tgggcgattt tgtcgttgcc agcgccgggc cgcacctcga atccgtcatt
    48241 gccggtcacg gtgccggggc acaaaccctt tctgaacaac ggctgtgtcg ggtactgaat
    48301 attgacatcg gcggtggcac tgcgaactac gccctgttcg atgccggaaa aatcagcggc
    48361 actgcctgcc tcaacgtcgg tggtcgcctg ctggaaaccg acagccaggg gcgcgtggtt
    48421 tacgctcata aaccggggca gatgattgtg gatgagtgct tcggcgcagg caccgacgcc
    48481 cgttcgctga ccggcgcgca gctggtgcag gttacccggc ggatggcaga actgattgtc
    48541 gaagtgattg acggaacgct ctcgccgctc gcgcaggcat tgatgcaaac cggtttgctg
    48601 cccgcaggtg ttacgcccga aatcattacg ctttctggcg gcgtgggcga atgttatcgc
    48661 caccagcccg ccgacccgtt ctgttttgcc gatattggcc cgctgctggc aacggcgctg
    48721 catgaccatc cgcgcctgcg tgagatgaac gtacagtttc cggcgcaaac cgtgcgcgcc
    48781 acggtgattg gcgcgggcgc gcataccctt tcgctctctg gcagcacaat ctggcttgag
    48841 ggcgtacaac tgccgctgcg caatttgccg gtggcgatcc cgattgatga aacggatctg
    48901 gtgagtgcct ggcaacaggc gctgcttcag ctggatcttg atcccaaaac tgacgcgtac
    48961 gtgctggcgc ttcccgcctc gctgcctgtg cgttacgccg cggtactgac ggtcatcaac
    49021 gcgctggtcg atttcgtcgc gcgttttcca aatccgcatc ccctgctggt ggtggccggg
    49081 caggactttg gtaaagctct gggcatgttg ttgcgcccac agctacaaca actcccgttg
    49141 gcagtcattg acgaagtgat tgtccgcgcg ggggactata tcgacattgg tacgcctctt
    49201 tttggcggat cggttgtgcc ggtgacggtg aaatcactcg catttccttc ctgagggaac
    49261 gacttatgaa actaaagacc acattgttcg gcaatgtata tcagtttaag gatgtaaaag
    49321 aggtgctggc taaagccaac gaactgcgtt cgggggatgt gctggcgggc gttgcagcgg
    49381 caagctcaca ggagcgcgtg gcggcaaagc aggtgttgtc ggaaatgacc gtagcggaca
    49441 tccgcaataa tccggtgatt gcctatgaag atgactgcgt gacgcggctg attcaggacg
    49501 atgttaacga aacggcctac aaccagatta aaaacaggag catcagcgaa ctgcgtgagt
    49561 atgtgctgag cgatgaaacc agcgtggacg acattgcctt tacccgcaaa gggctgacct
    49621 cggaagtggt cgcggcggta gcgaagattt gctccaacgc ggacctgatc tacggcgcga
    49681 agaaaatgcc ggtaatcaaa aaggccaata ccaccatcgg tattccgggc accttcagcg
    49741 cccgtttgca gccaaatgac acccgtgacg acgtgcaaag tatcgctgca caaatctacg
    49801 aagggctttc cttcggggtg ggcgatgcgg tgatcggcgt taacccggta actgacgacg
    49861 tggaaaactt aagccgcgtg ttggacacca tttacggcgt gatcgacaaa ttcaacatcc
    49921 caactcaggg ctgcgtactg gcgcacgtca ccacccagat cgaagcgatt cgtcgcggcg
    49981 cgcctggcgg actgattttc cagagtatct gtggcagcga aaaagggctg aaagagtttg
    50041 gcgtggaact ggcgatgctc gacgaagcgc gcgcagtggg tgctgagttc aaccgtatcg
    50101 ccggggaaaa ctgcctctac ttcgaaaccg ggcaaggttc ggcgctgtct gctggcgcta
    50161 acttcggcgc agactaggtg acgatggaag cacgtaacta tgggctggcg cgtcattacg
    50221 atccgtttat cgtcaacacc gtggtcggct ttattgggcc ggagtatctc tacaacgacc
    50281 gccagattat ccgtgctggc ttagaagatc actttatggg caagctgagc ggcatctcta
    50341 tgggctgtga ctgctgttac accaaccacg ctgatgctga ccagaacctc aacgaaaacc
    50401 tgatgatcct gctcgccacc gcaggctgca actacatcat ggggatgccg ctgggcgatg
    50461 acatcatgct caactatcag accaccgcat tccacgacac tgccactgtg cgtcagttac
    50521 tcaacctgcg cccgtcaccg gagtttgaac gctggctgga aagcatgggc attatggcaa
    50581 acggtcgcct gaccaaacgg gcgggcgatc cgtcactgtt cttctgatga cgcggagata
    50641 acacatcatg gatcaaaaac agattgaaga aattgtacgc agcgtgatgg cgtcaatggg
    50701 acaagcggcc cccgcgccgt cagaagcaaa gtgcgccacc actaactgtg cggcaccggt
    50761 gacctcggaa agctgcgcgc tggatttagg ttctgcggaa gcaaaagcgt ggattggtgt
    50821 tgaaaacccg catcgcgcag acgtattaac agaactgcgc cgcagcaccg tggcccgcgt
    50881 ttgtaccggt cgtaccggtc cgcgtccacg tacgcaggcg ctgctgcgtt tcctggccga
    50941 tcactctcgt tcgaaagata ccgtactgaa agaagtgccg gaagagtggg tgaaagcgca
    51001 gggcttgctg gaagtacgct ccgaaattag cgacaaaaat ctctacctga ctcgcccgga
    51061 tatgggccgc cgcctgtgtg cagaagctgt tgaagcgctg aaagcacagt gtgttgctaa
    51121 tccggacgta caggtcgtta tttctgatgg cctgtcaact gatgccatca ccgtgaacta
    51181 cgaagagatc ctgccgccgc tgatggcggg cctgaaacag gccgggctga aagtcggcac
    51241 gccattcttt gttcgttatg gtcgcgtgaa gattgaagat cagatcggtg agatcctcgg
    51301 cgcgaaagtg gtgatcctgc tggtggggga acgtccgggg ttgggtcagt cagaaagcct
    51361 ctcctgttac gccgtttact cgccgcgtat ggcgaccacc gttgaggccg atcgcacctg
    51421 catctctaac attcaccagg gcggcacgcc gccggttgaa gccgcagccg tcattgtgga
    51481 tttggcgaaa cgtatgctgg agcagaaagc atccggcatc aacatgaccc gataaggagg
    51541 catcatgcca gctttagatt tgattcgacc gtcggtaacc gctatgcggg tgattgcctc
    51601 cgttaacgct gaatttgcgc gtgaactaaa attgccgccg catattcgta gcctcgggct
    51661 gatttctgct gattccgatg acgtcaccta cattgcggct gacgaagcga ccaagcaggc
    51721 gatggtcgaa gtggtatatg gccgctcgct gtatgcggga gcagcacacg gaccgtcacc
    51781 gactgccggt gaagtgctga ttatgcttgg tgggccaaac ccggcggaag tgcgtgctgg
    51841 tctggatgcg atggttgcgc atattgaaaa tggcgctgct ttccagtggg ctaatgacgc
    51901 agaaaacacc gcattcctgg cacatgtagt ttcgcgtacc ggttcttatc tttcatcaac
    51961 cgccgggatc acgcttggcg atccgatggc gtatctggtg gcaccgccgc tggaagcgac
    52021 ctacggcatt gatgcagcgt tgaaatctgc cgacgtacag ctggtgacct atgtcccgcc
    52081 accgtctgaa accaactact cggcagcatt tttaaccggt agccaggccg cgtgtaaagc
    52141 agcctgtaac gcctttaccg atgcagtgct ggaaatcgcg cgtaatccaa tccagcgtgc
    52201 gtaacggagg ttgccgatga tcaatgcact gggattgctg gaagtggacg gcatggtcgc
    52261 cgcgatagat gcagcggatg ccatgctcaa agcagctaac gttcgtctgc tcagtcacga
    52321 agtgcttgac cctggtcgct taacgctggt ggtggaaggc gatctggcgg cgtgtcgtgc
    52381 ggcgctggac gcaggttgtg ctgccgcgat gcgtaccggg cgtgtcatca gccgcaagga
    52441 gatcggtcgg ccagacgatg acacccagtg gctggttact ggctttaacc gccagccgaa
    52501 gcaacccgta aaggaacccg acgcgccagt tatcgtcgcg gaatctgctg acgagttgtt
    52561 ggcgctgtta acatcagtac gtcagggaat gacggcagga gaagtggctg cccactttgg
    52621 ctggccgctg gaaaaagcca gaaatgcgct cgaacagctc ttttctgccg ggacgttacg
    52681 taaacgcagt agtcgttatc gtctcaagcc ccattaacct gtcggaggtg ccgggtgtcc
    52741 tacaacaccc ggcattaaca tcatgaaaaa gacccgtaca gccaatttgc accatcttta
    52801 tcatgaaccc ttacccgaaa acctgaagct cacgccgaag gtcgaagtgg ataatgttca
    52861 tcaacgacag acaacggatg tctatgaaca tgctttgacg attaccgcct ggcagcagat
    52921 ttacgatcag ctgcatccgg gcaagtttca tggtgaattt acggaaattc tgctcgatga
    52981 tattcaggtt tttcgtgaat acaccggtct ggcgctgcgt cagtcgtgcc tggtctggcc
    53041 gaactcgttc tggtttggca ttccggcgac gcgcggtgag cagggattta tcggttcgca
    53101 atgtctggga agcgcagaaa tagcgacgcg ccccggtgga accgagtttg aattaagtac
    53161 gccggacgat tatacgatcc tgggcgtagt gctgtctgaa gatgtcatca cccgtcaggc
    53221 taactttttg cacaatccgg atcgggtatt acatatgctg cgtagccagt cggcgctgga
    53281 agtgaaagag cagcataaag ccgcgctgtg gggctttgtc caacaggcgc tggcgacgtt
    53341 ttgcgagaac ccggaaaatc tccatcagcc tgcagtgcga aaagtgctgg gggataattt
    53401 gctaatggcg atgggggcga tgctggaaga cgcgcaacca atggtgacgg cggaaagcat
    53461 cagtcatcag agttaccgta gattactttc ccgcgcccgt gaatatgtgc tggaaaatat
    53521 gtctgagctg gtgacggtgc tggacttgtg taatcaactg catgtcagtc gccgcacgct
    53581 acaaaacgcg tttcacgcta ttttaggcat tggcccaaac gcgtggctga aacgcattcg
    53641 cctgaacgcc gtacgccgcg aactgataag cccgtggtcg caaagcacaa cggtaaaaga
    53701 cgccgccatg cagtggggat tctggcatct ggggcaattt gccacggatt accagcagct
    53761 gtttgccgag aagccgtcgt tgacgttgca tcagcggatg cgggaatggg ggtgagggag
    53821 ttacacccaa tccctgacct taataaactc acttaacgcc gcttctgggc tgccatcttt
    53881 tggctgataa tcatactccc agcgtaccag tggcggcatt gacatcagga tcgactcggt
    53941 gcgcccgcca gtttgcaggc caaacagagt gccgcgatcc cagaccagat tgaactcgac
    54001 ataacgaccg cgacggtaga gctgaaaatt gcgctcgcgc tcgccgtagg ccatcgcttt
    54061 acgtcgctct acaattggta aataagcgtc ggtgtagcct ttgcctaccg cctgcataaa
    54121 ggcaaaacag tggtcgaaat ctggcgtgtt cagatcatca aagaacagcc cgccaatacc
    54181 gcgctgttcg ttgcgatgtt tgaggtagaa gtaatcgtcg caccactttt tgtaacgggg
    54241 ataaacgtct tcaccaaatg gcaggcacag gtcacgggcg gtgcggtgcc agtgaatggc
    54301 gtcttcttca aaaccataga aaggggttaa atcgaagccg ccgccaaacc accagacggg
    54361 ctctgcaccc ggtttttcgg caataaaaaa acgcacattc gcgtggctgg tgggaacata
    54421 cgggttatgc ggatgcacta ccagtgaaac gcccatcgcc tcgaaactgc gcccggcaag
    54481 ttccgggcga tgagcggtgg cggaagcagg catcgcctcg ccgtggacgt gagaaaagtt
    54541 gacgcctgcc tgttcgaaaa caccaccatt acgcaacacc cgactacgcc cgccgccgcc
    54601 agcttcgcgc tgccaactat cttcgacaaa ttctgcgcca tcgacggcgg tcagctgctg
    54661 acaaatcgta tcctgaaggt tgagcagaaa ctgtttaacc tggtgtgcgt cgggtttcat
    54721 aacttatcgc tttttcgaat gtgctttctg gttgtcgaac cagtggaaat aactgatcac
    54781 gccttcagca atcgctgtgg cgattttctg acgaaacgcc gccgtgccta acagccgctc
    54841 ttcttccggg ttggtgataa acgaggtttc caccagcacc gaaggaaccg acggcgattt
    54901 caacaccaca aacgcggctt gttcggtgtt gcggctgtgc agtttatgca ccggcttaat
    54961 ctttttcaga atatgcgagc caagcgtcag gctgttttta atagtatcgg tttgcaccag
    55021 atcaaacagc acttgctgca ataggtgatc cttgtcagtc gcctttttac cggcaacttc
    55081 atcggcgcgg ttttcgcgtt cagacaggta tttcgccatt gcgctacttg ccccacggtt
    55141 agagagggca aataccgaag caccggcagc tttcgggttg gtaaagccat cggcatgaat
    55201 tgacataaac agatctgcgc catgtttatg ggcgatttca acgcgatcgt aaagtgggat
    55261 aaacgtatcg ccagaacgcg ttaaacgcgc atcaatccca tgattacgca aaatggaacg
    55321 gacgttttta gcaatcgcca gcaccacatg tttttctttc gaaccgttgc gtccgatcgc
    55381 tccggtatca attccgccgt ggcctggatc gagaacaacg acacgtttgc cgccagattt
    55441 tttggctttc ggcttgctgt gtccgttgct ggtttttaaa ggttcgtctt tggcgatggc
    55501 ttgcgacatt cctgacaacg tcagggcagc caaaccggct ttcagcacct ggcggcgcga
    55561 agtgagtgtt tttagtggtt taaaagtgct catacggcct gagttgtaat aataaagttc
    55621 cagatgttat atcgtatcgc gtaatccgtt acgacggttt gatgttgaga attgttttac
    55681 ttttcatttc aatagttgac aactccccat tatgccactt tttcaaccga ttttcgtcag
    55741 atattggcta attcgaccgt tcacgccata atcagccttt taacccgcaa aaaggtaacg
    55801 aataccatgg agatacgcgt atttcgccag gaagatttcg aagaggtcat caccctttgg
    55861 gagcgttgcg acttgctgcg tccgtggaac gatccggaaa tggacatcga gcgtaagatg
    55921 aaccatgacg tcagtttgtt tttggtcgct gaggtaaacg gtgaagtggt cggaacggtg
    55981 atgggcggtt atgacgggca tcgcgggtct gcttattatc ttggcgtgca tccagagttt
    56041 cgtgggcgtg ggattgccaa tgcgttgctt aatcggctgg agaaaaagct gattgctcgt
    56101 ggctgcccga aaattcagat caatgtgccg gaagataacg acatggtgct cggaatgtat
    56161 gaacgcctgg gatatgaaca cgccgacgtg ctgagtctgg gtaagcgttt gattgaagat
    56221 gaagagtact gagttccatc ctgtccatta tgatgcgcac ggacgcctgc gtttaccctt
    56281 gctcttctgg cttgtgctat tgcttcaggc gcgaacctgg gtgctgtttg tcattgccgg
    56341 tgcgtcgcgc gagcagggta ctgcgctgct aaatctgttt tatcccgatc acgataattt
    56401 ctggctgggg ctgattccag gcattccggc ggtgctggcg tttttgttga gcggtcggcg
    56461 ggctacattt cctcgcactt ggcatgtgct ctattttctg ttgctgttgg cgcaggtggt
    56521 tttactttgc tggcaaccgt ggctgtggct gaacggtgaa tccgttagcg gtatcggtct
    56581 ggcgctggta gtggcggata ttgtggcgtt aatctggctg ctgaccaatc gacgtttacg
    56641 cgcttgtttt aatgaagaaa aagaataacg gcactttttg gtgaatttgc actccaagca
    56701 acgttattga ataaccaaag gaagtgacat gaaatcgctg cgtttaatgt tatgcgctat
    56761 gccgttgatg ctgaccggct gttccacgat gtcgtcagtt aactggtcgg ccgctaaccc
    56821 gtggaactgg tttggatcgt ccaccaaagt gagcgagcag ggcgtgggtg aattaacggc
    56881 gtccacacca ctgcaagaac aagccattgc tgatgcgctt gatggcgatt atcgcctgcg
    56941 cagcggaatg aaaaccgcga acggcaacgt ggtgcgcttt tttgaagtga tgaaaggcga
    57001 caacgtggcg atggtgatta acggcgatca aggcacgatc agtcgcattg acgtgctgga
    57061 tagcgatatt cctgctgaca ccggtgttaa aatcggtaca ccgtttagcg acctttacag
    57121 caaagcattt ggcaattgcc aaaaagctga tggtgatgat aatcgtgccg tcgaatgtaa
    57181 agccgaaggc agtcaacata ttagctacca gttcagcggg gaatggcgtg gtcctgaagg
    57241 gttaatgcct tcggacgata ccctgaaaaa ctggaaagtc agtaaaatta tctggcggcg
    57301 ttaatttgcg tctgaacaaa ccgccgccgc gaaaaaacac gtaaaatagc gctcaacaat
    57361 gccacggatt gcgtggcatt cttattttca ggaggaacaa tgtctcaggt tcagagtggc
    57421 attttgccag aacattgccg cgcggcgatt tggatcgaag ccaacgtgaa aggggaagtt
    57481 gacgccctgc gtgcggccag taaaacattt gccgacaaac tggcaacttt tgaagcgaaa
    57541 ttcccggacg cgcatcttgg tgcggtggtt gcctttggta acaacacctg gcgcgctctg
    57601 agcggcggcg ttggggcaga agagctgaaa gattttccgg gctacggtaa aggccttgcg
    57661 ccgaccaccc agttcgatgt gttgatccac attctttctc tgcgtcacga cgtaaacttc
    57721 tctgtcgccc aggcggcgat ggaagccttt ggtgactgca ttgaagtgaa agaagagatc
    57781 cacggcttcc gttgggtgga agagcgtgac ctgagcggct ttgttgacgg tacagaaaac
    57841 ccggcgggtg aagagacgcg tcgcgaagtg gcggttatca aagacggcgt ggatgccggc
    57901 ggcagctatg tgtttgtgca gcgttgggag cacaacctga agcagctcaa ccggatgagc
    57961 gttcacgatc aggagatgat gatcgggcgc accaaagagg ccaacgaaga gattgacggc
    58021 gacgaacgtc cggaaacctc tcacctcacc cgcgttgatc tgaaagaaga tggcaaaggg
    58081 ctgaagattg ttcgccagag cctgccgtac ggcaccgcca gtggcactca cggtctgtac
    58141 ttctgcgcct actgcgcgcg tctgcataac attgagcagc aactgctgag catgtttggc
    58201 gataccgatg gtaagcgtga tgcgatgttg cgtttcacca aaccggtaac cggcggctat
    58261 tacttcgcgc cgtcgctgga caagttaatg gcgttgtaag cgtttctgat tcgatgatta
    58321 aaggccagcc agtaacaaaa ctgactggcc tttttattac ttttgcttca acgccgcata
    58381 cacctgatca actacccaac cataagttgc aatcggtaac tgaccgcttt cgaacatatt
    58441 gggatttttt tgtggatcgg caactggcga atggggcttg ttgcttaaca tcacaatcgc
    58501 catatgattg accggatcga taacggttac tgtcccggtc cagccagtgt gaccgtaggt
    58561 ttgcgggctt gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat tcacgcgcca
    58621 gccgaggcca aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc
    58681 actgaacagc tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc
    58741 aatatcgccg gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa
    58801 ggctttttca tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat
    58861 cacgccatcg cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc
    58921 tttcagcagc gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc
    58981 aacatagcgg tcaagcggct gaccggtaac cgactcgacg ataaatccaa gcagcatata
    59041 atcgacatcg ctgtagatat gtttgctgcc gggctgatat tccagcggcg tgcgcttgat
    59101 catctccagc gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg
    59161 gtattgcgga tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt
    59221 gtttttgcct ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat
    59281 ccgatcgtca ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc
    59341 gtacattttg gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg
    59401 ttccatcaac acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat
    59461 ctgattatct ttaatgatca gcaggtttac gccgggataa ccgacatcaa tttgctggct
    59521 aatccagcga tccatctggt taagccgttc gacgttaaac cctgctttct ctggcgagct
    59581 ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag
    59641 atagagcatt gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg
    59701 tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccatgg
    59761 ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc taccagacca gacggcccaa
    59821 aggcgctgtt taagcccatc ggcagacccc atcaggctat caggccgata aacaaaccgc
    59881 ccgccgcgcc gcctaaacag gcggtaacaa acggcttcat gcggggcagg gtgacaccgt
    59941 aaatcagcgg ttcaccaacg cccagcaggc cgggaataat cgccccgcgt acctgactgc
    60001 gtagcgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg
    60061 cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctagctgtcc attaacgcga
    60121 ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg
    60181 ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt
    60241 cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca
    60301 gggtgattaa cgaggtcagc agcatgtcga gatcgtccga cataaagcgg cgcaccatgc
    60361 cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat
    60421 cgatgggcag accaaagaaa tcgtgaaaac cggcgtagtc accggtggtt gcagcggggt
    60481 tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg
    60541 cctgggcggc gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca
    60601 ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca
    60661 ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca
    60721 gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg
    60781 cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc
    60841 ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct
    60901 gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat
    60961 caaccagtga actgtcatgt acacccagac gcaggcgcgt catacagtta ccacaactgg
    61021 cgatatttcc cagtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct
    61081 ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca
    61141 ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac
    61201 agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt
    61261 ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca
    61321 tcaggttgcc gaacactttg ccggatttaa tcatcagccc ggtggaaagc atattgagca
    61381 ccagtttctg cgctgtgcct gctttcattc gcgaagaacc agtgaccact tcggcaccta
    61441 caatcggtgt aatggcaaac tcggcggtgg tggaaacggc actccccgga ttacaggaaa
    61501 ttcccactgt gcggcaaccg agctggcggg cgtattccag tccggcaatc acatacggcg
    61561 tacgaccgct ggcggcaata ccgaccacca catcctgtgc cgttaaattt atatttttca
    61621 gatcgttaat gccgccttct cggctatctt ccgtgccttc caccgcgtgc tgaatggcat
    61681 attcgccgcc agcaatcaaa ccaaccacca gacccggttt cacgccgtag gtgggcggac
    61741 attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac
    61801 gaccgccgcc gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagga
    61861 cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca
    61921 gcgtcgatac gcggtcaatt tcagccgagg cggtgttcga gccttcagta atcatctttt
    61981 caagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc
    62041 atatatcccg aattgacaga cttaaaggaa tcattgagtc tacacttgca gagtgtgaag
    62101 agcgtcataa gaacttaagc ggaatttttg ctgccagccc gggagagtgg aagatgttgt
    62161 acctgacgaa aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg
    62221 actttctgcg ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac
    62281 agctcggcat cagtcagtcg agcattgtga aatttgccca gaagctcggc gcgcaaggat
    62341 ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg
    62401 ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc
    62461 gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgtgcgttg ttcgactacg
    62521 ctcggctgca aaaaatcatt gaggtcatca gtaaagcacc gtttatccag ataacgggcc
    62581 tgggcgggtc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc
    62641 gcgtggccta tgaagccgac acacacgtac aagccaccgt ttcccaggcg ttgaaaaaag
    62701 gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag
    62761 aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc
    62821 tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct
    62881 cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg
    62941 tgcaactcaa tgatgtggag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac
    63001 gcctgaaatg atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat
    63061 ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat
    63121 taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc
    63181 attgcaggga attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat
    63241 cttgctggat atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca
    63301 tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa
    63361 acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg
    63421 tctgggcagt ttcctcgata tgagcgatga cgatcgcgat ttccatattg acatcaatat
    63481 taaaggcgta tggaacgtca cgaaggcggt gctgccggag atgattgccc gcaaagatgg
    63541 tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc
    63601 gtacgcctta acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc
    63661 gcagtctggt attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga
    63721 aagcattgcc cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa
    63781 agcaatcccg ctgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct
    63841 cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac
    63901 actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg
    63961 gggaggattt cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg
    64021 gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca
    64081 ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgcgctg
    64141 gtcgcttctc tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat
    64201 gacgtctccc gcgagctgtt tgccgccctg aacccgccgt ttgagcaaca atggaccaaa
    64261 gaaaacggcg gcgacaaact gacaataaaa caatctcatg ccgggtcatc aaaacaggcg
    64321 ctggcaattt tgcagggctt aaaagcagac gttgtcactt ataaccaggt gactgacgta
    64381 caaatcctgc atgacaaagg caagctgatc ccggcggact ggcaaacgcg cctgccaaat
    64441 aatagctcgc cattctactc caccatgggc ttcctggtgc gtaagggcaa cccgaagaat
    64501 atccacgact ggaacgacct ggtgcgctcc gatgtgaagc tgattttccc gaacccgaaa
    64561 acgtcgggta acgcgcgtta cacctatctg gcggcatggg gcgcagcgga taaagctgac
    64621 ggtggcgata aagccaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg
    64681 ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg agcgcggcct gggcgatgtg
    64741 ctgatcagct tcgagtcgga agtgaacaac atccgtaaac agtatgaagc gcagggtttt
    64801 gaagtggtga ttccgaaaac caacattctg gcggaattcc cggtggcgtg ggttgataaa
    64861 aacgtgcagg ccaacggtac ggaaaaagcc gccaaagcct accttaactg gctctacagc
    64921 ccgcaggcgc aaaccatcat caccgactat tactaccgcg tgaataaccc ggaagtcatg
    64981 gataaactga aagacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc
    65041 tcctggccgg aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta
    65101 gcggcggggc gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct
    65161 taagcctcgg caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc
    65221 tggtgatgca gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc
    65281 aggtagtagc ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg
    65341 gcgtttttgg cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc
    65401 tgcttgatgc gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc
    65461 tagcctcgct cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca
    65521 aagtcaccta tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg
    65581 tggtacgtac cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcag
    65641 aaacgcttgg cgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gaactttctc
    65701 cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg
    65761 tgatttttat cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg
    65821 tgcgcttaca ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg
    65881 catctctgct gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg
    65941 taggtcatta atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg
    66001 gggcaaatgg tttctgattg gcatcgggat gctggtatcg gcgttcatcc tgctggtgcc
    66061 gatgatttat atcttcgtac aggcgttcag caaagggctg atgccggttt tacagaatct
    66121 ggccgatccg gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt
    66181 accggtaaac ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc
    66241 tggacgccag ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc
    66301 cggtctggtg tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga
    66361 acataatctg caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac
    66421 gtgtccgttt gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggaaga
    66481 cgaagcggcg attttgcttg gcgcgtctgg ctggcagatg tttcgccgcg tcacattgcc
    66541 gaacattcgc tgggcgctgc tttatggcgt ggtgttgacc aacgcccgcg caattggcga
    66601 gtttggcgcg gtgtcggtgg tttccggctc gattcgcggc gaaaccctgt cgctgccgtt
    66661 acagattgaa ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact
    66721 gttaacgctg atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga
    66781 gaatcaggaa aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta
    66841 agaagtcgtt tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc
    66901 agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg
    66961 ccgggctgga gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc
    67021 tgcacgcgcg cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata
    67081 tgacggtatt cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gatcgcccga
    67141 atgccgcagc catcaaagcg aaagtgacaa aattgctgga gatggtgcag cttgctcatc
    67201 tggcggatcg ttatccggca cagctttccg gcggccagaa acagcgcgtg gcgctggcgc
    67261 gcgcgctggc tgtagaaccg caaattctgc tgcttgatga accgtttggc gcgctggatg
    67321 cgcaggtgcg taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca
    67381 ccagcgtgtt cgtgacccac gaccaggaag aagcgacgga agtagctgat cgtgtagtgg
    67441 tgatgagcca gggcaatatt gaacaggctg atgcgccgga gcaggtatgg cgcgaaccag
    67501 cgacccgttt tgtgctcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg
    67561 gcgggcagtt ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg
    67621 ggccggtgga tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg
    67681 attcgccgct gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag
    67741 tggtgcagcc gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg
    67801 ccccgcagcg tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg
    67861 acgagcgtat cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg
    67921 agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt
    67981 gtaacctgta gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata
    68041 gagatcgtga gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga
    68101 atggggccgg ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt
    68161 tcggtgaaag atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt
    68221 aaaccgggtg atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg
    68281 attgccgcgc tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa
    68341 cgccgtgcgg cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc
    68401 atggaaggtg cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc
    68461 gatcagttca ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc
    68521 tggcagcaaa ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact
    68581 atcaccggcg tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg
    68641 cagccggaag aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg
    68701 gggattttca acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa
    68761 aacaccatgc gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc
    68821 gcggttgccg gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc
    68881 atctgcgatc gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagt
    68941 cagggggcgg ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattaag
    69001 gcatgtaaaa aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc
    69061 ttcccggtaa tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg
    69121 cggttacggg tattgccgag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt
    69181 tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg
    69241 ttgatgcggg cttcttcatg aacagttaac ttcgcgtaag cggggagtgt cattagtagt
    69301 gtaatgacca gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc
    69361 gatttgactg ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag
    69421 gagaaaaaat taaatgagta gtttgttgtt gtttaacgat aagagtaggg cgctgcaggc
    69481 ggatatcgtc gccgtgcagt cgcaggtagt gtacggcagc gtaggcaaca gcattgcggt
    69541 gcctgctata aaacagaacg gcttgaatgt ctttgccgtg ccgacggtat tgctgagcaa
    69601 tacgccgcat tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta
    69661 tttgcgtgcg cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta
    69721 tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga
    69781 ccatcctgac ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta
    69841 tgtcaaacct gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcaaggaat
    69901 tacccccaat atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag
    69961 tgccattgct gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag
    70021 cgcctccggt aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt
    70081 gaatgtcatt tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg
    70141 tgctcagctt atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc
    70201 ggggttgcgc gtactggaag tgatgcgcta tacccagcag catgagagcg atgaattgat
    70261 tttgccgccg ctggcggaag cataaaaaat ggcgccgatg g
//
LOCUS       DAJTXQ010000005        60565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000005 DAJTXQ010000000
VERSION     DAJTXQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.005"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            188..982
                     /gene="panB"
                     /locus_tag="OO515_000306"
     CDS             188..982
                     /gene="panB"
                     /locus_tag="OO515_000306"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6574334.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            994..1845
                     /gene="panC"
                     /locus_tag="OO515_000307"
     CDS             994..1845
                     /gene="panC"
                     /locus_tag="OO515_000307"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6574335.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2137..3033)
                     /gene="rpnC"
                     /locus_tag="OO515_000308"
     CDS             complement(2137..3033)
                     /gene="rpnC"
                     /locus_tag="OO515_000308"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6574336.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3307..3687
                     /gene="panD"
                     /locus_tag="OO515_000309"
     CDS             3307..3687
                     /gene="panD"
                     /locus_tag="OO515_000309"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6574337.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3691..4920)
                     /locus_tag="OO515_000310"
     CDS             complement(3691..4920)
                     /locus_tag="OO515_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6574338.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4984..5424)
                     /locus_tag="OO515_000311"
     CDS             complement(4984..5424)
                     /locus_tag="OO515_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6574339.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5529..6299)
                     /locus_tag="OO515_000312"
     CDS             complement(5529..6299)
                     /locus_tag="OO515_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6574340.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6296..7222)
                     /locus_tag="OO515_000313"
     CDS             complement(6296..7222)
                     /locus_tag="OO515_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6574341.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7331..7993
                     /gene="can"
                     /locus_tag="OO515_000314"
     CDS             7331..7993
                     /gene="can"
                     /locus_tag="OO515_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6574342.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8034..8570)
                     /gene="hpt"
                     /locus_tag="OO515_000315"
     CDS             complement(8034..8570)
                     /gene="hpt"
                     /locus_tag="OO515_000315"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6574343.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            8776..11166
                     /gene="gcd"
                     /locus_tag="OO515_000316"
     CDS             8776..11166
                     /gene="gcd"
                     /locus_tag="OO515_000316"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6574344.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11213..12763)
                     /gene="cueO"
                     /locus_tag="OO515_000317"
     CDS             complement(11213..12763)
                     /gene="cueO"
                     /locus_tag="OO515_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6574345.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12929..13276
                     /locus_tag="OO515_000318"
     CDS             12929..13276
                     /locus_tag="OO515_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6574346.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13382..14248
                     /gene="speE"
                     /locus_tag="OO515_000319"
     CDS             13382..14248
                     /gene="speE"
                     /locus_tag="OO515_000319"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6574347.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14264..15058
                     /gene="speD"
                     /locus_tag="OO515_000320"
     CDS             14264..15058
                     /gene="speD"
                     /locus_tag="OO515_000320"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6574348.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15096..15458)
                     /locus_tag="OO515_000321"
     CDS             complement(15096..15458)
                     /locus_tag="OO515_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6574349.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15633..18230)
                     /gene="acnB"
                     /locus_tag="OO515_000322"
     CDS             complement(15633..18230)
                     /gene="acnB"
                     /locus_tag="OO515_000322"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6574350.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18585..20342
                     /locus_tag="OO515_000323"
     CDS             18585..20342
                     /locus_tag="OO515_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6574351.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20413..21837)
                     /gene="lpdA"
                     /locus_tag="OO515_000324"
     CDS             complement(20413..21837)
                     /gene="lpdA"
                     /locus_tag="OO515_000324"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6574352.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22162..24054)
                     /gene="aceF"
                     /locus_tag="OO515_000325"
     CDS             complement(22162..24054)
                     /gene="aceF"
                     /locus_tag="OO515_000325"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6574353.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24069..26732)
                     /gene="aceE"
                     /locus_tag="OO515_000326"
     CDS             complement(24069..26732)
                     /gene="aceE"
                     /locus_tag="OO515_000326"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6574354.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26893..27657)
                     /gene="pdhR"
                     /locus_tag="OO515_000327"
     CDS             complement(26893..27657)
                     /gene="pdhR"
                     /locus_tag="OO515_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6574355.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28198..29571
                     /gene="aroP"
                     /locus_tag="OO515_000328"
     CDS             28198..29571
                     /gene="aroP"
                     /locus_tag="OO515_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6574356.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29614..30468)
                     /gene="ampE"
                     /locus_tag="OO515_000329"
     CDS             complement(29614..30468)
                     /gene="ampE"
                     /locus_tag="OO515_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6574357.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30465..31016)
                     /gene="ampD"
                     /locus_tag="OO515_000330"
     CDS             complement(30465..31016)
                     /gene="ampD"
                     /locus_tag="OO515_000330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6574358.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31104..31997
                     /gene="nadC"
                     /locus_tag="OO515_000331"
     CDS             31104..31997
                     /gene="nadC"
                     /locus_tag="OO515_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6574359.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32200..32640
                     /gene="ppdD"
                     /locus_tag="OO515_000332"
     CDS             32200..32640
                     /gene="ppdD"
                     /locus_tag="OO515_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6574360.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32650..34036
                     /gene="gspE"
                     /locus_tag="OO515_000333"
                     /pseudo
     CDS             32650..34036
                     /gene="gspE"
                     /locus_tag="OO515_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34026..35228
                     /gene="hofC"
                     /locus_tag="OO515_000334"
                     /pseudo
     CDS             34026..35228
                     /gene="hofC"
                     /locus_tag="OO515_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35263..36306)
                     /gene="guaC"
                     /locus_tag="OO515_000335"
     CDS             complement(35263..36306)
                     /gene="guaC"
                     /locus_tag="OO515_000335"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6574361.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36462..36506)
                     /locus_tag="OO515_000336"
     CDS             complement(36462..36506)
                     /locus_tag="OO515_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574362.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36531..37151
                     /gene="coaE"
                     /locus_tag="OO515_000337"
     CDS             36531..37151
                     /gene="coaE"
                     /locus_tag="OO515_000337"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6574363.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37151..37894
                     /gene="zapD"
                     /locus_tag="OO515_000338"
     CDS             37151..37894
                     /gene="zapD"
                     /locus_tag="OO515_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6574364.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37904..38101
                     /gene="yacG"
                     /locus_tag="OO515_000339"
     CDS             37904..38101
                     /gene="yacG"
                     /locus_tag="OO515_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6574365.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38201..38590)
                     /gene="mutT"
                     /locus_tag="OO515_000340"
     CDS             complement(38201..38590)
                     /gene="mutT"
                     /locus_tag="OO515_000340"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6574366.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38650..41355)
                     /gene="secA"
                     /locus_tag="OO515_000341"
     CDS             complement(38650..41355)
                     /gene="secA"
                     /locus_tag="OO515_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6574367.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41417..41929)
                     /gene="secM"
                     /locus_tag="OO515_000342"
     CDS             complement(41417..41929)
                     /gene="secM"
                     /locus_tag="OO515_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6574368.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42160..43077)
                     /gene="lpxC"
                     /locus_tag="OO515_000343"
     CDS             complement(42160..43077)
                     /gene="lpxC"
                     /locus_tag="OO515_000343"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6574369.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43178..44329)
                     /gene="ftsZ"
                     /locus_tag="OO515_000344"
     CDS             complement(43178..44329)
                     /gene="ftsZ"
                     /locus_tag="OO515_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6574370.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44390..45652)
                     /gene="ftsA"
                     /locus_tag="OO515_000345"
     CDS             complement(44390..45652)
                     /gene="ftsA"
                     /locus_tag="OO515_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6574371.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45649..46479)
                     /gene="ftsQ"
                     /locus_tag="OO515_000346"
     CDS             complement(45649..46479)
                     /gene="ftsQ"
                     /locus_tag="OO515_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6574372.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46481..47401)
                     /gene="ddlB"
                     /locus_tag="OO515_000347"
     CDS             complement(46481..47401)
                     /gene="ddlB"
                     /locus_tag="OO515_000347"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6574373.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47394..48869)
                     /gene="murC"
                     /locus_tag="OO515_000348"
     CDS             complement(47394..48869)
                     /gene="murC"
                     /locus_tag="OO515_000348"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6574374.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48923..49990)
                     /gene="murG"
                     /locus_tag="OO515_000349"
     CDS             complement(48923..49990)
                     /gene="murG"
                     /locus_tag="OO515_000349"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6574375.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49987..51231)
                     /gene="ftsW"
                     /locus_tag="OO515_000350"
     CDS             complement(49987..51231)
                     /gene="ftsW"
                     /locus_tag="OO515_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6574376.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51231..52547)
                     /gene="murD"
                     /locus_tag="OO515_000351"
     CDS             complement(51231..52547)
                     /gene="murD"
                     /locus_tag="OO515_000351"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6574377.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52550..53632)
                     /gene="mraY"
                     /locus_tag="OO515_000352"
     CDS             complement(52550..53632)
                     /gene="mraY"
                     /locus_tag="OO515_000352"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6574378.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53626..54984)
                     /gene="murF"
                     /locus_tag="OO515_000353"
     CDS             complement(53626..54984)
                     /gene="murF"
                     /locus_tag="OO515_000353"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6574379.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54981..56474)
                     /gene="murE"
                     /locus_tag="OO515_000354"
     CDS             complement(54981..56474)
                     /gene="murE"
                     /locus_tag="OO515_000354"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6574380.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56461..58227)
                     /gene="ftsI"
                     /locus_tag="OO515_000355"
     CDS             complement(56461..58227)
                     /gene="ftsI"
                     /locus_tag="OO515_000355"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6574381.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58243..58608)
                     /gene="ftsL"
                     /locus_tag="OO515_000356"
     CDS             complement(58243..58608)
                     /gene="ftsL"
                     /locus_tag="OO515_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6574382.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58605..59546)
                     /gene="rsmH"
                     /locus_tag="OO515_000357"
     CDS             complement(58605..59546)
                     /gene="rsmH"
                     /locus_tag="OO515_000357"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6574383.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59548..60006)
                     /gene="mraZ"
                     /locus_tag="OO515_000358"
     CDS             complement(59548..60006)
                     /gene="mraZ"
                     /locus_tag="OO515_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6574384.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14463 a        16776 c        14916 g        14410 t
ORIGIN      
        1 caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag tactttccag
       61 gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg taattgataa
      121 atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc atttatcagg
      181 acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg ataaaaagcg
      241 tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg atgaaggact
      301 taacgtcatg ctggtaggcg actcgctggg catgacggtt cagggtcacg actccaccct
      361 gccggtgacc gttgccgata tcgcctacca cactgcctcc gtacgtcgtg gtgcgccaaa
      421 ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac aagcctttga
      481 aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg gcggtgagtg
      541 gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg
      601 tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc gcggcgatga
      661 agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg cacagctgct
      721 ggtgctggaa tgcgtgccga ttgaactggc aaaacgtatt accgaagcac tggcgatccc
      781 ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga tgcacgacgc
      841 cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg ccgaaacggg
      901 cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttatccggg
      961 cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg
     1021 ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact
     1081 atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat
     1141 gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga agatctggca
     1201 cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa agtggattta
     1261 gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca tacttacgtt
     1321 gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca ttttcgcggc
     1381 gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc ctgcttcggt
     1441 gaaaaggact ttcagcaact ggcgctaatc cgcaaaatgg ttgccgatat gggcttcgat
     1501 attgaaattg tcggcgtccc gattatgcgc gccaaagatg gccttgcgct cagttcccgt
     1561 aatggttatc tgacggcgga acaacgcaaa attgcgcccg gtctgtacaa agttttaagt
     1621 tcaattgccg acaaattgca ggctggcgag cgggatcttg atgaaattat tgccattgca
     1681 gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg cgatgccgac
     1741 acgttgctgg aagtctctga aaccagcaaa cgagctgtaa ttctggtagc cgcctggctt
     1801 ggcgatgccc gcctgatcga caacaaaatc gtcgaactgg cgtaatgctt ttactctaaa
     1861 taattcaagt tgcaggaagg cggcaagcga gtgacaaatt cgtcaggaac gaatttgccc
     1921 agccgcaggc tggccttcgg tgagagacat ggatgtctct cattaatccc caggagctta
     1981 cataagtaag tgactggggt tagcgaacgc agacgcagca catgcaattt aaagtatgac
     2041 gagtataact ggcgctacgg ctgatggcgc caattattaa tttgccccac gtcatggcga
     2101 tggcgtctgt aagcaattat tcataacgca gacaacttag ttaatcagcc tatcaatctc
     2161 agcaagagat aaatttgtca tctctgcaac gtcttcccga gacatccctt tactcagaaa
     2221 acgctgggcg aattcttgcc tttcctcctg tcttccctgc tgaatcccct tctcaatccc
     2281 tttctcttca aaccactgcg ccagcgtcat catagactcc cctcccgttt ccctgtctct
     2341 cagcacacca taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg
     2401 catggcaact aactgacttc cgctagtgta cccttcgtca atcagcgtga ccagttgctc
     2461 cagcaataac attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat
     2521 ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg
     2581 actgttatag acgcgtcgtg ccagctccgg cgagtaaaat atatcaaacc agcacattga
     2641 gagcggataa ggtgtggcct cgccctgata aaacagtatc ggcaccacca gcggcagctt
     2701 atcgtgatcc gcctccagat gccggtgcat ggcagcaata gaataacgca tcatgcgaaa
     2761 gaccattttc ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt
     2821 gccctgcatt tgcacggaat agagcacgtc cgtgctgtgt cctttcaggc tctcttcaat
     2881 gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa agttctcgta attccactgg
     2941 caaatgtatc tccagaaagt cgcgagccgt ctccgcgtgc attaaaaatt gtttaaacac
     3001 tgcatcatgc ggtgtggtac tcggtgcatc catcttccct gccttccgtg ttgtcttgcg
     3061 tgaaggcagt atccagtcaa ggcgtgccat cgtcagccac ctctttagtg tttaccgagc
     3121 agcgttcaga gaaatttcaa ttggttacag ccgttacatg gcacgcttcg gcacggtacg
     3181 caaactgctg atgtaaatgc cgtcagatga gtagacacta aacaaaaatc gggcaatact
     3241 gcgtgagaat ttcctcaaag caggcatcgc ctgcttcgtt aacgacaggg tagaaaggta
     3301 gaagttatga ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg
     3361 gacctgcact atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt
     3421 ctcgaaaacg aagccattga tatctggaat gtcaccaacg gcaagcgttt ctccacttat
     3481 gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc
     3541 gccagtgtcg gcgatattgt catcatcgcc agcttcgtta ccatgccaga tgaagaagct
     3601 cgcacctggc gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg
     3661 aaagcgattc cggtacaggt tgcttgattg ttatccctgc ggctggttac tcaccagccg
     3721 cgacatcgtc tccagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt
     3781 atcccccggt tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt
     3841 tgctgccttc acggctttgt cattaaatcc gccaaacgga tacgaaagat accagacatg
     3901 cggattaaat tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc
     3961 actacggctc agtaatatgg gtcggcgata gccatccacc cgatgcaaaa aatgggtatg
     4021 tgactgaaaa tcaaaaacat cgcgaatttc gttaagctca gaaacgctca taaattgcag
     4081 cgattttggg ttccacttct gcgggtgacg tttgatgcgc gaggtaacaa caaacgccgt
     4141 cgccttcatg ccatattgtt tcaatacagg atacgcatag cggctcaccg acttgaggcc
     4201 atcatcaaag gtaatcacca ccgctcgcgc agggagattg atcttattct tcacgtaacc
     4261 ttccagctgc gccatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatttggtt
     4321 attgaaagcg cgtaccgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcgtc
     4381 gcgcagaata tggtgataag ttagcaccgg aaggccatta tcgggttggg catccagcgc
     4441 gctgatatag gccagtcgat cgccaatacg gacctgatac caggtttgat ttaacctgtc
     4501 tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc aataccccaa atggcgcact
     4561 gcccacactc ggcgcgttat agaccggcgt atctttccag gtaactaagt tctgattact
     4621 cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaaccggctc
     4681 gagatgccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt aataacttgc
     4741 ggcagtgggc tccacggcaa tgatttgtcc ggcccgaata ttccccacgg tcaccatctt
     4801 gtcgccaatt tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa
     4861 cgcggcactg acacttgcgg taaacagcat cagcaggaga ataacagctt gtttgtacat
     4921 gaaaaaataa ccgagggtga aacaacgctt ttattgtagc aaaagcgcta taagaatcgt
     4981 gggttaatac tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgttggtg
     5041 ccaaagacta ctcacctccg gcccacccag ttcgacaata cgctcgcgaa actctgaact
     5101 ggtcatggtt tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat
     5161 cacttcgcaa cgggagtgtt tgtggcttaa taatgaagcc acgcggtacg gtggcgctcc
     5221 ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca
     5281 catcatgcgg ctgagcatat tagagcttaa cccacgccag aaattcacgg cccggcactg
     5341 aagaagcgcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg
     5401 acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga
     5461 ccattgttga taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg
     5521 aaatagcctt agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa
     5581 aacgccacaa taaagaccac cagtacgcca aaggtagtga ccagcggaac atcattgata
     5641 ccgaggaagc cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc
     5701 ccttgccaga acggcggcaa caaagtcagt gagtaaaaga ccccacccaa atacgtgagt
     5761 ggcgttaaca caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacaccg
     5821 ttcagcaaac ccgcaaggga gaacaacacc gccgtgagca ccagggttaa ggcaacgaat
     5881 acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca
     5941 acaaacagac cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga
     6001 accggcgcta ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac
     6061 gcaacgttgg cgtaggcatt ggtgatcacc gacatcatga tcagccccgg tacgatgaac
     6121 tgcatatagc tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaagata
     6181 ataaagtaaa gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata
     6241 aagcgatgga tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg
     6301 cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt
     6361 tgttacgcat acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga
     6421 tcccctgctc acgcagcact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat
     6481 agccatcgag cttcggtaac ggacttttcg gtgcgagatc gagaataaag gtttccgatt
     6541 tcagcttcgc cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa
     6601 tgccgatatt gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg
     6661 tggtgccttt gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat
     6721 ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg
     6781 caatcattaa acggcgcttc atcccgccag ataacatacg cgcacgttcg ttgcgttttc
     6841 cccatagatc gagttgttta agatattttt cgctgcggat gtacgcctct ttgcgctcca
     6901 caccgtagta ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt
     6961 taaattcctg cggcaccagt cccaactgac gtttagcgtt cacgacatct ttctcgagat
     7021 cgtaaccaaa tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga
     7081 tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga
     7141 cctgcaaatc tattccacga agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt
     7201 gaagttccag tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt
     7261 ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc
     7321 gttaacctcc atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat
     7381 gctggtggaa gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt
     7441 tctatggatt ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc
     7501 gggcgaactc tttgttcacc gtaatgttgc taacctggtc attcacaccg acctgaactg
     7561 cctttccgtg gttcagtatg cagtggatgt actcgaagtt gaacacatta ttatctgtgg
     7621 ccactacggt tgcggcggcg tacaagccgc cgttgaaaac ccggaactgg ggcttatcaa
     7681 caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat
     7741 gccgcaagag cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtttataa
     7801 cctaggccac tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca
     7861 cggctgggcc tacggcattc acgacggctt gctgcgtgat ctggatgtca cagcgaccaa
     7921 ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc
     7981 caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg
     8041 tcccgcagaa tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg
     8101 ccgtaaccca ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc
     8161 acttcacgac gggacggttt atccagcagc gtacaaatcg ccagcgactt cggttcgcgc
     8221 aggcttaaga tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca
     8281 atcagcacgt ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg
     8341 gtggtggaca tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc
     8401 tgaacttcac ggcacaggtc cgccataaac ataaatgagc cacgcagcag acccaccagc
     8461 accatatcgc tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata
     8521 cgcgctttaa tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc
     8581 atatgattta aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa
     8641 gacacgcagt ataccagtaa aactattcat tcgcggaatc tgttaataaa gctcgaattg
     8701 tgatgacggt cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg
     8761 aaatggtgtc tctttatggc aattaacaat acaggctcgc gacgattact cgtcacgcta
     8821 acagcccttt ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc
     8881 gcgattggcg gctcctggta ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg
     8941 atgctgtggt gcagtaaacg cgccgcgctt tggctatacg cagccctgct gctcggcacc
     9001 atgatttggg gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgat
     9061 attctggtct tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt
     9121 cctgccagcg gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tagtatcctg
     9181 acctgggccg gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca
     9241 cctgcggaag ctatctcacc cgttgccgat caggactggc ctgcctatgg tcgcaatcag
     9301 gaaggtcaac gcttttcacc tctgaaacaa attaacgccg ataacgtcca taatctgaaa
     9361 gaagcctggg tgttccgtac tggcgatgtg aagcagccga acgatccggg cgaaatcacc
     9421 aatgaagtga cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc
     9481 ctgtttgcgc tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa
     9541 actaacgagt ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca
     9601 gaaacggctt cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat
     9661 gatggtcgac tgattgcgat taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat
     9721 aaaggcgtgc tcaatctgca aagcaatatg ccagacacca aaccgggtct gtatgaaccg
     9781 acttcgccac cgattatcac cgataaaacc atcgtgatgg ccggttcagt taccgataac
     9841 ttctcaaccc gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg
     9901 ctgtgggctt ttgatcccgg cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc
     9961 tttaccttta actcgccaaa ctcctgggca ccagcggcct atgacgcgaa gctggatctg
    10021 gtctatctgc cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa
    10081 caggaacgtt atgccagctc gattctggcg ctgaatgcca ctaccgggaa actggcgtgg
    10141 agctaccaga ccgttcacca cgacctgtgg gacatggatc ttccggcaca gccgacgctg
    10201 gcggacatca ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc
    10261 aacatttttg tgctcgatcg tcgcaatggc gaactggtgg tttcggcccc ggaaaaaccg
    10321 gtgccgcaag gtgctgcgaa aggcgattac gttactccga cccagccgtt ctctgaactg
    10381 agcttccgtc cgaagaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac
    10441 caactggtgt gccgcgtgat gttccaccag atgcgctatg aaggcatttt cacgccgcca
    10501 tctgaacagg gcacgctggt cttcccgggt aacctgggaa tgtttgaatg gggcgggatt
    10561 tccgttgatc caaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg
    10621 aaactgatcc cacgcggtcc gggtaacccg atggagcagc cgaaagatgc caaaggcacg
    10681 ggtacggaat ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg
    10741 ttcctctcac cgtttggtct gccgtgtaaa cagccagcat ggggttatat ctcggcgctg
    10801 gatctgaaaa tcaatgaagt ggtgtggaag aaacgtattg gtacgccgca agacagtatg
    10861 ccgttcccga tgcctgtacc ggtgccgttc aatatgggta tgccgatgct gggcgggcca
    10921 atctccacgg cgggtaacgt gctgtttatc gctgctacgg cagataacta cctgcgcgct
    10981 tacaacatga gcaacggtga aaaactgtgg cagggccgtt taccagcggg tggtcaggcg
    11041 acgccaatga cctatgaagt gaatggcaag cagtatgtgg tgatctccgc aggcggtcac
    11101 ggttcatttg gtacgaagat gggcgactat attgtggctt atgccctgcc ggatgatgtg
    11161 aaataaccat aatgcccgga gagatccggg catatttccg aatacggtct ttttataccg
    11221 taaaccctaa catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag
    11281 catgttcttt cggtgcgttg tgattaaact tcaccagcac ttcactgaca ttgccttcta
    11341 ccttaacggt atctttccag cccgcgcgat gagccgctgg cggtttgcca ttttctgaca
    11401 agatacggaa ctgcatgccg tgaatatgga acggatgcag catcatgtcg cccacgccag
    11461 agataaccca acgttcgtac tgccctttcg ccgccgcaaa catcggctta ttcatatcaa
    11521 acgcctgacc gttgatttta ttggcatggt ggaaatcgaa cttcccgccg tggttcatat
    11581 gattcatatt gccgtgcccc atatggccca tcatctggct gtgatccatt ccggccatcg
    11641 cctgatcgcc atatttctcc attagcatct gcatccccat catatcgagc atcgggtcca
    11701 tagaaagttg cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac
    11761 ttaatgtgtc aggcaaagca ccggaggcac taatagcaat cggctgaatc cgcattaccg
    11821 gatgcggctt gtcaaacggc gcaatcgcca tccccatctg gctgaccggt agcgtcacca
    11881 gatcaaaggg tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca
    11941 ccggcagctc gctcaccttc accggttcag gtagcagacc accgtcgctg gcaatcacat
    12001 acagcgggcg attgtcgctg gtggcgaaat tgagcgagcg ggcgttacag ccattgagca
    12061 aacgcaggcg cagccagcca cgcggggcag cgtgttgtgg gtagattgca ccgttggtca
    12121 gcagcgtatc gccaaaccag cccacggcgg cggtcatcac atccagttga taatcaatct
    12181 gcccatcagc gttaaatttt ttatcctgaa caatcaccgg aacatcatcg ataccccact
    12241 gttttggcag cattaatttc aggatctcgt catcttcaat taccaccagc ccagccaacc
    12301 ccatcgccac ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag caggtagcgg
    12361 caggttgatc aacgttcaac gtcaccgtac gtttgccgcc tggcggaata attccctgcg
    12421 ggccgccgtc gacgtcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg
    12481 ccagttggtt atagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc
    12541 ccagcagatt gccgttatag ccccaggtag ttgcagtttt ctcgccaaag gtagactgac
    12601 ctgcgccaat agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg
    12661 gtaacgttgg gcgttccgcc gcaaagactg cgcggctcca cagcggcaag gctgaagcca
    12721 cacccagcgc gacggaatat tttaagaaat cacgacgttg catagttatt tccttattct
    12781 taagcaggcg aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag
    12841 ccgcagacac aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt
    12901 tatcttcttt aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg
    12961 cagcctgatg gccgtctgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat
    13021 ggccgattta acggcagttt ttgtctttct gaagaacgat tgtggttacc agaacttacc
    13081 taacgggcaa attcgtcgcg cactggtctt tttcgcccag caaaaccagt gggatctcag
    13141 taattacgac accttcgaca tgaaagccct cggtgaagac agctaccgcg atctcagcgg
    13201 cattggcatt cccgtcgcta aaaagtgcaa agccctggcc cgcgattcct taagcctgct
    13261 tgcctacgtc aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt
    13321 caatgttagc tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc
    13381 catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc
    13441 ggtagataac gttctgtatc atgaaaagac cgatcaccaa gatctgatca tttttgagaa
    13501 cgctgcattt ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga
    13561 gtttatctat catgagatga tgacccatgt tccgctactg gcccacggtc acgcgaaaca
    13621 tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa
    13681 cgttgagtca atcacgatgg tggaaatcga tgcgggtatc gtgtcgttct gccgtcagta
    13741 tctgcccaac cataacgccg gtagctacga cgatccgcgc tttaagctgg tgatcgacga
    13801 tggcgtcaat ttcgttaatc aaaccagcca gacctttgat gtcattatct ccgactgcac
    13861 cgatcctatc ggtcccggcg aaagtctgtt cacctcagca ttttatgaag gctgcaaacg
    13921 ttgcctgaat cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga
    13981 agaagccatc gacagctatc gcaaactcag ccattacttc agcgacgttg gcttttatca
    14041 ggcggcgatc ccgacctatt acggcggtat catgactttt gcatgggcga cagataacga
    14101 cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa
    14161 atgccgttat tacaatccgg caatccatac ggcagctttt gccttacctc agtatctgca
    14221 agacgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact
    14281 gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc
    14341 caaaactgcc gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa
    14401 ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat
    14461 cgcccgccag gattacgaac cacagggtgc cagcgtcact attctggtga gtgaagaacc
    14521 ggttgacccg aaactcatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt
    14581 tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg
    14641 cggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct
    14701 gaaggcgctg aattacctga tccaccagct tgagtccgat atcgtaacca ttgattatcg
    14761 cgtgcgcggt tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa
    14821 ttcgattcag aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtgaa
    14881 cgtctatcag gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca
    14941 ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc
    15001 tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgccag ctgtttaacg
    15061 gctctggcgg agctcccagg ctccgccaga tttatttact tctgctgcac gaaattgcgg
    15121 taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct ttcttcgtcg
    15181 tagtagttca tcccttcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc
    15241 atcacctctt caccgtccat ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt
    15301 tcgctacctt tcagtgcgtg cgctgcttgt tccacttcat caagcaaagc caggttttct
    15361 ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat acgcaccttt
    15421 accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcccggc
    15481 aatgcgccga aatcgcattg cgctattgat gtaattattg cagccttacc ggagaaaaac
    15541 agcggaacgg cgatggttta gaaataaaaa atgcccggga atctcattca ccgggcattg
    15601 tgtcgtttat gcgcagcgcg tgcgcttact tattacaccg cagtctggaa aatcacccca
    15661 tcggcttttt cggtgtactg agaaagctgg ttgaagttca gataacgata agtatcaacg
    15721 gcagttttat ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca
    15781 atcagcgccg caacagccgc cagttctgca gaagccagga agacattcgc gccagtcccc
    15841 agacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc
    15901 gcctggttac ccatacacag agaacagccc gggatctcga tacgagcccc gctcttaccg
    15961 aagacgctgt agtagccttc ttcagtcaac tgtgcggcgt ccatgcgggt tggcggtgcc
    16021 acccacaggc gggtcggcaa ctggcctttg tgcgcatcca gcagtttacc cgcagcacgg
    16081 aagtgaccga tgttagtcat gcaagaaccg ataaacactt cgtcgatctt ctcgccctgt
    16141 accgcagaca gcggacgtgc gtcgtccggg tcgttcggtg cacagaggat tggctcttta
    16201 atatccgcca gatcgatgtc gatcactgcc gcgtattcag catctgcatc ggcttccagc
    16261 agctcaggat tcgccagcca tttttccatg ccctgaatac gacgttcgag ggtacgacga
    16321 tcgccataac cttccgcgat catccacttc agcaggacga tgttagagtt caggtattcg
    16381 atgatcggtt ctttgttcag cttgatagta caaccagcgg cagaacgctc agcggacgca
    16441 tcggtcagtt caaatgcctg ctcaactttc agatccggca gaccttcaat ttccagaatg
    16501 cgaccagaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc
    16561 gcatacagcg ggatcgcgtg taccagatcg cgcagggtga tgcccggctg cattttgcct
    16621 ttgaagcgca ccagaacgga ttccggcata tccagcggca ttacgcctgt tgcggctgca
    16681 aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg ggtatgggag
    16741 tcaccaccgg taccgacggt atccggcagc agcatacggt tcagccagga gtggattacg
    16801 ccgtcacccg gacgcagcga cacaccacca cggttcataa tgaagtccgg cagcgtgtgg
    16861 tgcgtgttca cgtcaactgg cttcggatac gccgcggtat ggcagaaaga ctgcatcacc
    16921 aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt catcgggccg
    16981 gtggtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc cggacgaata
    17041 cctttcacac cgcaggcacg acctaccatt ttctgagcca gcgagaagcc gcgatcgctc
    17101 tcagcgacat ctttcgcctg acggaacaca tcactgtgcg gcagaccaag tgcttcacgc
    17161 gctttggtgg tcaggccacg cccgataatc agcggaatac ggccaccagc acgcacttca
    17221 tcaatcagca cgtcggtttt cagttcgaag gtcgccagca gttcgccggt ttcgtggtta
    17281 cgcacttcac ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag gttagagacg
    17341 tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt taaagaagat gggtgcaatt
    17401 ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc
    17461 ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc cacaacgtca
    17521 ccgacgtacg ccagcgggaa acctttctgt tgcagagctt cgatttgctt gatcgggcca
    17581 acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg
    17641 tgcagtggga tatccgggcg cgaccacgca tccggtgccg gagaaaggtc atcagtgttg
    17701 gtttcgccag tgactttgaa gacggtaacg gtcagttttt cagccagcgc cgggcgattc
    17761 aggaaccatt cggcatccgc ccaggactgc attacctgct tcgcatattc gttgcctgct
    17821 ttggctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg agaaagtgct
    17881 ttggcagcga tcggtgccag tttggcatca tccagcgcgt cgatcagcgg atgaatgttg
    17941 taaccaccct gcatggtacc cagcagttcg atggcttttt ccggagtcag cagaggggat
    18001 ttggcttcgc ctttagcgat agcagccagg aagcctgctt tgacataggc ggcttcatcg
    18061 acgccagggg gaacacggtt ggttaataga tctaacagga attcttcttc gcccgcgggc
    18121 gggtttttca gcagctctac aagtgcggcc atttggtttg catccagggg tttgggcgca
    18181 atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc
    18241 ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt
    18301 ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga cgacgtgtta
    18361 atctgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg gtctagagat
    18421 tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat
    18481 cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattaac agcacctgcg
    18541 ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc
    18601 cgtttaaacc ccatgtgctg gcactaattt gcagtgccgg gctttgtgcc gcctctgccg
    18661 ggctatatat aaaaagccgc acagtggaag cgcctgtgga aacgcaatcg acacaacaga
    18721 ctgcgcctga catcaccgca gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc
    18781 ccgccgtcgt caaatccgca ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg
    18841 cgctgtaccc cgattctctg ctttcacaag tgttaatggc atcaacctat ccggcaaacg
    18901 ttgctcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat gctgctattc
    18961 aggcggtatc tgaccagccg tgggacgcca gcgttaaatc actggtggcc tttccacaat
    19021 tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg
    19081 cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg
    19141 gctcgctgaa gtcatcaacc gaacagaaag ttattaccac aacgaagaaa gctgtaccgg
    19201 taaaacagac agtcacggca cccgtcatac catccaatac cgttttaact gccaaccccg
    19261 tcattacaga gccggcaaca accgtcattt ccattgagcc cgccaatcct gatgtggtct
    19321 atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact gcgtatccgc
    19381 cggtttatct gccaccacca gccggagaac cgtttgttga cagctttgtg cgcggattcg
    19441 gctatagcat gggcgttgct accacgtacg cactatttag cagcatcgac tgggatgacg
    19501 acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat ggcggtcatc
    19561 gtgacggtaa tggctggcaa cacaacggcg acaacatcaa tatcgacgtc aacaatttca
    19621 accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac aatccaaact
    19681 accgtaatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat caaaccgatg
    19741 tcaacggcgg aatgagtgcc acgcagctac ctgctccaac acgcgacagc cagcgtcagg
    19801 cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattaca cgagataccc
    19861 aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc tatgacgact
    19921 tccgcgactt cagccgtcgc caaccactga cccagcaaca aaaggacgcc gctcgtcagc
    19981 gttatcaatc ggcctctcct gagcagcgcc aggcggttcg cgagaaaatg cagactaacc
    20041 cacagaacca gcagcgaaga gaggcagcgc gtgagcgtat tcagtctgca actcccgaac
    20101 agcgccaggt gtttaaggaa aaagtacagc agcgcccact gaaccaacag caacgtgata
    20161 acgcccgcca gcgtatacaa tcggcatcac ctgaacaacg tcaggttttt cgggaaaaag
    20221 ttcaggagag ccgcccacaa cgtctaaacg acagtaacca tactgtcagg ctgaataacg
    20281 agcaacggtc agcagtacgc gaacgtctct ctgagcgcgg agcaaggcga caggaaaggt
    20341 aaattgcaga cgtaaaaaaa gcggcgtggt tagccgcttt tttaattgcc ggatgttccg
    20401 gcaaacgaaa aattacttct tcttcgcttt cgggttcggc aggtcggtaa tgctaccttc
    20461 gaacacttct gccgccaggc ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag
    20521 tgcgatgtct tcagcatcac aacccatttc gattgccaga ccgatttcac ccagcagctc
    20581 accgccgtta gtaccgacaa tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat
    20641 cagcttggtc ataccgtctg cgcagtcgga agcgatagca cgaccagaag cagcccacgg
    20701 gaaggtggcg gtttcatagc tgatgccttt ctctttcgct tctttctcag tcagacctac
    20761 ccatgcaact tctggttcgg tataggcgat agacgggata actttcggat cgaagtagtg
    20821 ttttttaccg gcgataactt cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag
    20881 catcggctga ccaacgatat cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg
    20941 tttgtcaacg cggatgaaac cacggtcgtc cacttccacg ccagctttgc ctgcgtcgag
    21001 gtttttaccg ttcggcacac gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc
    21061 agcgggtgct tttttgcctt ccatcgtcac ataaataccg tcttctttcg cttcaacggc
    21121 ggtaactttg gtttccagca tcaggttgaa tttcttgctg atacgcttgg tgaagacttt
    21181 aacgatgtct ttgtcagctg ccgggataac ctggtcgaac atttcaacca cgtcaatctg
    21241 tgaacccagc gcgtggtata cggtgcccat ttccagaccg atgataccgc cacccattac
    21301 cagcaggcgt tctggtactt ctttcagttc cagcgcgtca gtggagtccc agatacgcgg
    21361 atcttcatgc ggaataaacg gcagttggat cgggcgagaa cccgctgcaa tgatcgcatt
    21421 gtcgaagttg atcacggttt tgccgttctc accttcaact tccagggtgt tagccccggt
    21481 gaatttaccc agaccgttga ccactttgac tttgcggcct ttcgccatac cagccagacc
    21541 accggtcagc tgattgatca ctttctcttt ccaagtacga atcttgtcaa tatcggtttt
    21601 cggttcgccg aagacgatac cgtgttcagc cagcgctttg gcttcttcga taacttttgc
    21661 tacgtgcagc agtgctttag aagggataca gccgacgttc aggcaaacac cgccaagggt
    21721 gttgtaacgt tctacgatta cggtttccag acctaaatca gcgcaacgga aggcagcgga
    21781 gtaacctgcg gggcctgccc caagtaccac gacctgagtt ttgatttcag tactcatcat
    21841 gacctctata tatttatctc cggcggtcat acccgtcgtc tttcaggccg cagcggcgtt
    21901 agcttcacgc cctcacccca gtcacttact tatgtaagct cctggggatt ctggcgcttg
    21961 ctaccttgct gcacccagaa atccataggg tatgtgaagt aataccctaa ccaccaccgg
    22021 gtcgttctat ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa caaacggcaa
    22081 ccgatttgtc tattcgctaa taacctcaat acattcatga gattaccaga aaaaagccgg
    22141 ccgttgggcc ggctctttta cttacatcac cagacggcga atgtcagaca gcgtgttgtt
    22201 aatgatggta atgaaacggg caccatcagc accgtcgatc acgcggtggt cgaaggagag
    22261 agaaatcggc agcatcagac gcggcacgaa ctctttacca ttccacaccg gctccatcgc
    22321 ggacttggaa acgccgagga tagccacttc cggcgcgttc acaatcggcg cgaagtgggt
    22381 agtacccagg ccgccgatgc tggagatggt gaagcaaccg ccctgcattt cgcccgcagt
    22441 cagcttaccg tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca gctcgatgat
    22501 gcctttcttg ttgacgtctt tgaataccgg aacaaccaga ccgttcgggg tatccaccgc
    22561 cacaccgatg ttgatgtatt tcttcagggt cagacgctga ccgtcttccg acagcgaact
    22621 attgaagcga ggcatctgct caagagctgc agcaacggct ttcatgatga agacaaccgg
    22681 ggtgagcttc acatccagct tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc
    22741 ttccagctcg gtgatatcgg ttttgtcgaa gtgggtaaca tgcgggatca tcacccagtt
    22801 acggctcagg ttagcaccag aaattttctg gatacggccc agttccactt cttcgatttc
    22861 gccaaacttg ctgaagtcca ccttcggcca cggcagcatg ccagggatac caccgccagt
    22921 cgccgccgga gctgcttctg cacgtttgat agcttctttc acgtaagcct gaacgtcttc
    22981 gcgcaggata cgacctttac gaccagtgcc cttcactttc gccaggttaa cgccaaactc
    23041 gcgtgccaga cggcggatca gcggagtcgc gtgaacgtaa gcgtcgtttt cagcaaattc
    23101 agatttgcct tcggctttcg cagccggagc tgctgccggg gcttcagctt tcgctgccgg
    23161 agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc
    23221 gaagatcata atcagcgagc cagttttcac tttatcgcca acgttgactt tcagttcctt
    23281 cacgacgcct gcaaacggag ccgggacctc catagaagct ttatcgcctt ctacggtgat
    23341 aagcgattgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac
    23401 ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagccgctg atgcaggggc
    23461 cgctgccgga gctgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac
    23521 ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc
    23581 tttcacggtg ccagcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt
    23641 gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc
    23701 aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc
    23761 tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc
    23821 gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac
    23881 tttgatctct ttaacgatac ccgcctgcgg agacggaact tccatagagg ctttgtcgcc
    23941 ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc
    24001 ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct
    24061 tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt
    24121 gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca
    24181 accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg gctgtcggaa
    24241 cgaccgaagc catcagtacc cagtacgcgg tagtcgtcag ccggtacgta agtacggacc
    24301 tgctcagcga acagtttcat atagtcggta gatgccactg ccggagcgtc gttcatcacc
    24361 tgagcgatat acggaacgcg cggagtttcc agcggatgaa gcatgttcca gcgttcacaa
    24421 tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc agaacctacg
    24481 ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat agaaccggag
    24541 cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta
    24601 cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta
    24661 gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc
    24721 atgatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc
    24781 agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta
    24841 cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtcgccagc cgcccagcac
    24901 agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaacgg aatcatcggc
    24961 agattgttgg tgctgtaaga ggtcgccgct gccagccagg aacaccctgc gcccagctcg
    25021 ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg
    25081 cggtcctgcg ggatgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga
    25141 ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc
    25201 gacttgttct tcagcattac gttcagggca cgaacgaaag cgatagtggt agagatctct
    25261 ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg
    25321 gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg tgcgtgcaga
    25381 taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca
    25441 tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc
    25501 ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg
    25561 gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg
    25621 tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag
    25681 tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca
    25741 ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc
    25801 agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg
    25861 ataacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta
    25921 ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaagac caggttatcc
    25981 agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg
    26041 tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg gtgttccaga
    26101 tatttcagga atttagcctg gtaaatagca ccaatcggcc ccagacccat agatacggtc
    26161 gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg
    26221 tgaacttcct gacggaagtt atccagctgc tcttgagtca gacgaccttc caggaaagca
    26281 cgagcgtaca cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc
    26341 tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg
    26401 aaggacgcca tatggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg
    26461 atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac
    26521 tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt gcctgcggct
    26581 acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc
    26641 tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc
    26701 ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt
    26761 tgagttttct ggaacctgtc ccattgaact ctcgccggaa agctcaataa gacaggttct
    26821 acgtttagtt gccgcgcttt tatatacgct tgatttacaa catcttctgg ataattttta
    26881 ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct
    26941 ctcttcacga cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc
    27001 ttcgcgcgct tcttccggct taccggccat aatcgcttca aatatgcggg tgcggtgact
    27061 actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt
    27121 ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc
    27181 ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca gatcgccaga
    27241 ctgctgcgcc agctctatgg cgtggtggag ttcacggatg cgttccttgt cttcatcggt
    27301 actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa
    27361 gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg
    27421 ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc
    27481 ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag
    27541 ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagaa tcaaaaactc
    27601 cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag
    27661 ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc
    27721 taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt
    27781 gcacatttta acaacttgac atatataacg tttcaaaatt gtaactatgc acaaatgtag
    27841 actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc gaattgaacc
    27901 gattcactta ccaatttttt gatttttaag tcaataaaaa cgaatttaaa ttcattctgc
    27961 atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca actcatcttc
    28021 aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat ctgacggaag
    28081 tctttttgta acaattcaaa cttctttgat gtaaaaaaat taatacaaca aacggaattg
    28141 caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg
    28201 atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag
    28261 cttatcgcgc tgggtggcgc gatagggaca gggctattcc tgggtagcgc ctccgtaata
    28321 cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg
    28381 atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac
    28441 tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta
    28501 ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat tcagttctgg
    28561 tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat taacgccatc
    28621 aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt
    28681 attgcgatag tagcgatgat catcttcggc ggctggctgc tgttcagtgg caacggcggc
    28741 ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca cggcttcacc
    28801 ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga actggtgggg
    28861 atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcgac taaccaggtt
    28921 atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc actgatgccg
    28981 tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga gttaggcgat
    29041 acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc cgtgtacaac
    29101 agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg taatgcgcca
    29161 aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct ggtgtctgcg
    29221 ctggttacag cattgtgcgt attgattaac tatcttgctc cggaatccgc atttggcctg
    29281 ttaatggcac tggtggtatc cgcactggtg atcaactggg cgatgatcag tctggcgcat
    29341 atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt
    29401 tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg
    29461 atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt
    29521 atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac
    29581 gccctcaccc atgcagggtg agggcaataa tctttacacc aacgctccgt aaatcgtcag
    29641 tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa ccgctgcctt
    29701 cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg ccagacgcgt
    29761 taacacctga tactgcgaag tatggaagtc acccagcgaa gcaaaccagg ccggtaacgc
    29821 tttctcccca tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc
    29881 cagtacatga agcacggcat caatgccgga ctgtaaacga tgatgcggcg tctggtatcg
    29941 cgccagccag tattgccatg cacgcaaaaa cgcatacccc atcagcgtaa cgggtcccca
    30001 ggttcccccc acaatcaacc agaacagcgg tgcaagataa aaacgaaagt taatccacag
    30061 caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg
    30121 aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc
    30181 tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat
    30241 cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag
    30301 taaaaaagtc acgcccatcg caataatggt catgcctaac gtgcgcccga gagaaaaatg
    30361 tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct cgcccaactt
    30421 aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg
    30481 ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt tttccgatcc
    30541 ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat cgggatagcg atctatcagt
    30601 gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag agtatcggtg
    30661 ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cacgcccctg atactgagag
    30721 actcccgcat gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcacgg
    30781 cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc
    30841 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
    30901 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg
    30961 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
    31021 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
    31081 cgttagctat ctggagtttt aacatgccgc ctcgccgcta taaccctgac acccgacgtg
    31141 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
    31201 aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa
    31261 actctcgctc tcatgccacg gtgagcaccc gcgagaatgg cgtattttgc ggcaaacgct
    31321 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
    31381 atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg
    31441 tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca
    31501 gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg ttggatacgc
    31561 gcaaaacctt acctggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag
    31621 cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaatcat attattgcct
    31681 ccggctcagt gcgccaggcc gtcgaaaaag cgtcctggct gcacccggat gcgccagtag
    31741 aagtcgaagt agagaatctg gaagaacttg atgaagccct gaaagcagga gccgatatca
    31801 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
    31861 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa
    31921 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt
    31981 caatgcgttt tcgctaattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc
    32041 ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tcttttgctg caacgaaata
    32101 aaaaacctcg gaagctgctt tcccgtttcg ttttttgccc ctcgcttgtg ctgatggcgt
    32161 ttgccaaagt agcgccaacc aaatcaagga gcgaaacaga tggacaagca acgcggtttt
    32221 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagcgc cattggtatt
    32281 cccgcttatc aaaactacct gcgcaaagct gcactcaccg acatgctaca aacctttgtg
    32341 ccttaccgta cagccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
    32401 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
    32461 gcaaaaggcg tggtgtcgct gaccgggcaa gaaagtctca atggactaag cgtcgtcatg
    32521 acaccgggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg caatattcaa
    32581 agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa
    32641 ggagcggcaa tgaatattcc acagctcacc gccctgtgtc tgcgttatca gggagtcttg
    32701 ctggatgcca gcgaagaggt ggttcatgtt gcggtagtcg atgcaccttc gcatgagttg
    32761 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
    32821 caaatggaag gccacgccag tcgcacacaa cagacattgc ccgtagctgt tcaggagaag
    32881 catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga acaacgtgcg
    32941 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc
    33001 gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac cgccagatta
    33061 aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
    33121 gtcgaactgg caggaaacgc cgtctcattt cgtattgcga ccttaccatg tcggggtggt
    33181 gaaaaggtgg tattaaggtt gttacagcag gtgaaccagg cactggatgt taacacgctg
    33241 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
    33301 gtgctggtaa ctggccctac aggcagcggc aaaacggtca cgctttatag tgccctgcaa
    33361 aagctgaata ccgctgacat taatatttgt agcgtcgaag atccggttga gatccccata
    33421 gccggactaa accagacgca aatccatccg cgtgccgggc tcacctttca gggcgttttg
    33481 cgtgcgttat tgcgccagga tcctgacgtc atcatgatcg gagagatccg ctatggcttt
    33541 aacggcagaa attgccatta aagccgcgca aaccggtcac ctggtgttgt ctaccctaca
    33601 cactaattcc acctgcgaaa cgctggtacg tttacagcaa atgggagtcg cccgctggat
    33661 gctctcatca gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33721 ttgtcgccag cagcaagggg agcccatcca tattccagtc aatgtatggc cgtcgccgct
    33781 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc
    33841 cttatttgaa gttctgccca taacgccggt cattcgtcag cttatttccg ctaataccga
    33901 cgttgaatcg ctggaaacgc acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33961 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    34021 gccgcatggc gagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    34081 aagatgggat gctatgagca gagagccgtg ctttgctgct catggcacta cagcaacaga
    34141 tggttacccc acttagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34201 aaagcgcgga agtcattcat caactggcga cgctactcaa agccgggtta acgctttctg
    34261 aagggctggc actgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34321 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34381 aggtatttcc gccactctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34441 atgaatgctg ctttgaactg gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag
    34501 tgaaatcagc gttacgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34561 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34621 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggagctggc
    34681 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgaccga
    34741 cctggcttat agtgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatgc
    34801 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34861 cttttttgca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34921 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34981 ataccgggga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    35041 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    35101 cggataacct cgcagcctta ctggaaccga cgttgctgat cataacggga ggaattatcg
    35161 gtacgctggt ggtggcgatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35221 tgggataacg ctggcgtgtt gctccacacc agcgttggaa gattacaggt tgttgaagat
    35281 gcggttttct tgttcctgca cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg
    35341 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35401 ttcaaccggg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc gatattccgc
    35461 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35521 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35581 gccaagcatg acgaaatcgg caccgccgcc aaaggctttt gcgacatcgc ccggcgtggt
    35641 gcagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35701 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35761 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35821 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35881 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35941 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtaccggt
    36001 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    36061 ttcgacagaa tagtgtttat gcacagcagt caaaatatca aaagaagcca gcgcagaggc
    36121 catagaaaat gtacctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36181 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36241 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36301 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36361 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36421 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca
    36481 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atgaggtata
    36541 tagttgcctt aacgggaggc attggcagtg gcaagagtac cgttgccaat gcgtttgctg
    36601 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36661 cacctgcgct acatgccatt gctgatcact ttggcgctaa catgattgct gctgatggaa
    36721 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36781 ttaacgccct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcta
    36841 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36901 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36961 gcgatgatgt aactcgcgag catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    37021 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    37081 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37141 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37201 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37261 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37321 cgaagtccgc actgagctgt tgaaagaact tgaccggcag caacgtaaac tccagacctg
    37381 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37441 ggcggggagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37501 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37561 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37621 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37681 gtcggccccc ttccgtaaac aaaccagtct gaatggtttt tatcaggata acggtggcga
    37741 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37801 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37861 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37921 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37981 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    38041 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    38101 acatttgtct gatgccgcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38161 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38221 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgact agcgacatcc
    38281 actcaccggg ttgcccttct ttaccccacg gctccccttc ccagctttcg accagccaga
    38341 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38401 gttgtggggt aatcccgact tcttcctgaa gttcacgcac taccgcctgt tccggcgttt
    38461 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38521 cgcgacgcgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38581 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38641 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38701 gcaaggatcg ttacgtccta ctttgcgctc accggtttgc gccgccagtg cagctgcggc
    38761 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc gctaaacgct cggcttccat
    38821 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgga ctttgctcag
    38881 cgtgctgata acttcatatt ttaacgactc cagcatcgct gcaaacatgg agaacgattc
    38941 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    39001 cagatagtcc atcgccgcta ggtgctcttt ccacagagag tcgagagttt gcagcatgac
    39061 gcctttttcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39121 ttcgatggac tgcgccagaa ttcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39181 atccagccac tcggcaattg gcaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39241 ccccggaata tcccacattt cttccagtga ctgtggcgga atgtaggcat caatggtcgc
    39301 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39361 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39421 cagttgctta cgaatgtcga agttacggct ttcaacttta cgctgggcgt tggcaatcgc
    39481 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39541 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39601 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39661 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39721 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39781 cgcggcaact tctgcctgcc agctaccacc gagcacaata tctgtaccac gacccgccat
    39841 attggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39901 ggcgtggaat ttggcgttca ggacgttgtg cttaataccg gctttggtca gttcgtttga
    39961 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    40021 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    40081 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagctt
    40141 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40201 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40261 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgacggcc
    40321 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40381 gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40441 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40501 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40561 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40621 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40681 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40741 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatattgt cgcgcaggta
    40801 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40861 tgccggtgct ggcatgcccg gcaggttgat accgacagtc aggccaagga attcaaacag
    40921 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40981 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    41041 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    41101 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgtaccacgg cgaaagcttc
    41161 cgggatcaga ttttccagca cttcgccttt ttccagacgt gcacgaaact ctgcggtttt
    41221 ccctttcagt tcttcgtcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41281 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41341 caatttgatt agcataataa aatctcaaac gccccgcgtt gcggagttaa taaaatgaag
    41401 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41461 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41521 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41581 caagatgttg cgcctgaaga ggcaaagact cttcagcaac gggcagtgtt tgcggtgcca
    41641 ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg ttgatgccag taatcaacgg
    41701 aatagttcga attcgggcgg cgtgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41761 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41821 cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat ccctaataag agatgcggcc
    41881 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41941 cccgatcgta ttcaaacgta tttagcgcac aaatcttatc attaaagcct gcagtccaca
    42001 gcacgaatga agataagttg tgcggaaaag tgcttaagaa aacagcgttc gcaccgtttt
    42061 ttatagctct tataaaattc aaaaaaaaat gactggttca cctggccgga gagagtgcca
    42121 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42181 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42241 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42301 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42361 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42421 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42481 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42541 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42601 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42661 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42721 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42781 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42841 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42901 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42961 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agagtgactt tcttgccggt
    43021 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    43081 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43141 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgcagga
    43201 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacga
    43261 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43321 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43381 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43441 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43501 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43561 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43621 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atctcagaca
    43681 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcag
    43741 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43801 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43861 catgcttgga cagttcagtg atcccctgct ccgcgaatgc catacgcttc ttgccttcaa
    43921 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43981 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    44041 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcgac
    44101 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44161 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44221 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44281 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44341 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44401 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44461 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44521 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44581 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44641 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44701 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag cagctcggta tagcgcggct
    44761 cgatcacctc tgccagggtc tgacgttgca gactccgtgg cggacgaccg cctacgctcg
    44821 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44881 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44941 cattgccagc ataaggaatt accttagtgt ggcgcaatgc cccaccggta taaacggcga
    45001 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    45061 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45121 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45181 cttttgcctg catccgcacg cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45241 caatcgcata ctcttgcggg atcacatgca gcacacgatg ttcatcgcgc acacgcaccg
    45301 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45361 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45421 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45481 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45541 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45601 cggtaccaat ctccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45661 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45721 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45781 ctgctgctgt aaaaccggat aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc
    45841 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45901 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45961 gccctgcaac acttcattgg cgctgccttc cgggccatac agcattggaa gcacctgctt
    46021 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tatgttgatc
    46081 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46141 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46201 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46261 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46321 ttgcgcatct tccatccagc ccaacacgac ccagccgctc accaacactg tcgttaaaac
    46381 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46441 ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46501 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46561 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcgctgt
    46621 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46681 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46741 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46801 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46861 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46921 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46981 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    47041 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagcagtt
    47101 tgctgcgtag tttatccatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47161 agcccatcag ctcaagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47221 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47281 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47341 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcaatt ttatcagtca
    47401 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47461 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccgattaa taccggtgcc
    47521 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47581 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47641 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47701 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttactaggtt tttatccggc
    47761 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47821 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47881 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47941 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    48001 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc
    48061 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48121 taagtcgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48181 cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa aattaataaa agtctgtttt
    48241 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48301 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48361 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48421 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt ggttgtcgtt
    48481 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48541 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttatcg
    48601 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48661 ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg tgaccggatt tggcgctaaa
    48721 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48781 gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48841 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48901 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48961 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    49021 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    49081 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49141 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcaccgtta
    49201 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49261 taaattccgt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49321 gttgcgaacc tttaccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49381 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accagcacac
    49441 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49501 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49561 ctttggtggc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49621 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49681 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49741 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcagac
    49801 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49861 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg
    49921 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49981 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    50041 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    50101 gatcagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50161 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50221 gtcaatttct aacgctttgc ggccaatcga catcgcgcga aaagcgacga agaataccat
    50281 taaaagtgcc agcaccacac cgacataccc cagttcttcg ccgataatgg cgaaaataaa
    50341 gtcagtgtgc gcttccggca gatactccag tttttgtacc gagttaccta aaccttgtcc
    50401 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50461 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50521 tatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50581 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50641 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50701 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatataaca
    50761 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50821 acgcgatgcc cctttaaccg agctacccac taccagcacg atcatcagca ggatgataga
    50881 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50941 gatcgccaga atgaatgcca ggatcagata gacgccatca cgcttcgcga agaagaacgg
    51001 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    51061 cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51121 atctttttcc cgcgagccca tcacccagcc ttttagcgcc gtggagatcc agaccaggat
    51181 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51241 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51301 ctggcacagg ccggggagag cagaaccata tcgcccagct gaacacgcgg agccagcaag
    51361 cgcatcgctt gttccatggt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51421 tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt cgccattcag atagcgcgcc
    51481 agtggactga agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51541 acctgcaagc cattcaacgc cgcttccgta ctgccgacgt tagtggcttt cgaatcgtta
    51601 atccagcgta cgccattgtg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51661 gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag ctgcgtctgc cagtgccagt
    51721 gctgccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51781 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51841 cccatgttga cgccaaagct gacgcagcgt tcatcagcac cgcgaatcgg cattgttaag
    51901 gcatcatcag cattgacgac acaaactttc gcattttcgt aaatacgcaa ttttgccgca
    51961 cggtattgct gcaaaccaaa cggatagcgg tccatatgat cttcagtcac gttcagaatg
    52021 gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct ggaagctcga cagttccagc
    52081 acgtacagtt cgcactcagc atcaagcagc atcaacgctg gcaggccaat attgccaccc
    52141 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52201 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca gaacagctcg
    52261 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgcctgt
    52321 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52381 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52441 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52501 aggcccaggc cgataatgac gacattttta ccctgataat cagccatgat taacgtacct
    52561 tcagcgttgc cagaccaatc agaaccagca tcagcgaaat aatccagaaa cgcacaatga
    52621 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52681 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52741 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52801 ctaacgcacc acctaacgcc agcgaaccta catcgcccat aaagacctgc gccggatagg
    52861 tgttaaacca caggaagccc agtcctgccc cgactatcgc cgtgcagaca atgaccagtt
    52921 cccctgcgtg tcgcagatac ggtatatgca agtagctggc aaagttcata ttgccggtcg
    52981 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccaggccgt
    53041 cgagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    53101 gaatgtagaa cagccccagc tgcggcatca catctttaaa gaatgggacc accagctgcg
    53161 ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc gacacccagc gcaatgaccg
    53221 acatccagaa atacttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53281 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53341 cgtacggatt ggacgggtaa gcccacagca gtacggagat cacaatcgcc gtcaggatca
    53401 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53461 gcaccacctg accaaaggaa agtttttgca aatgagctat catacgcggg cccatccaca
    53521 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53581 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53641 ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta cgtgaaccct taactaaaat
    53701 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53761 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccacgct
    53821 taacacgcgg tcaataccag cagcttttgc cgcctcgccc acctgtacat ggcaggcttc
    53881 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53941 agccagtacc tgaacagcgg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    54001 cagcaactgg ttttctgcca gttggatggg gaacagacgg cctggaacag ctttcagatt
    54061 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54121 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54181 ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc acatggatat tggtggcggt
    54241 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54301 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54361 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54421 atgcgccgct gccaggttgt tgaccagcgc agcttccggg cgagtcagac tcacagtcca
    54481 ggctatttcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54541 caagcgcaac aacgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54601 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54661 ggaacctgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54721 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatat ccagcggacg
    54781 gctgaccagt agtgcgcccg cgccgccagc tttcgcctgg tcggcaaaat catgagcatc
    54841 gaaacgttcg cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54901 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag
    54961 ttggctaagg gttacgctaa tcatgcaatc acccccagca gacgcgccac cgtgacgcga
    55021 tcggagtagt ccagacgctg attgccaaca atctggtaat cttcatggcc tttgcccgcg
    55081 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55141 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55201 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55261 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55321 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55381 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55441 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55501 ccgagtgcca gcagtgtcgc cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc
    55561 agatggcttt caatttcgcc atcgccccaa cttgagctaa agcgaatcgt cgcaccgctg
    55621 tcgtgatagt tcacgtcgat cgcttttaac cagcgtccgt gacagttcgg attaatatga
    55681 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55741 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55801 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55861 accgacgccg caaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55921 tccattgcgc aaaacgtcgc gccctgctcc accagccccg ccagctcatg ctgaacatcg
    55981 actgcggaac cggtagtatt ttctgtcggg atcactttcc caagcagacc gttaccaacg
    56041 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag cagctgggta
    56101 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56161 tggtaaaagc ggcccgccag tgcagataaa cgctcattga gctggctgag atagatgacc
    56221 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc tttcgcctct
    56281 gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc
    56341 tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc
    56401 atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa
    56461 ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt tatcgcccgt
    56521 tgtcagcgca tccggctcga tgttcatggt acgcaatacg ccgcccatga tggcaccaaa
    56581 gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac
    56641 aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt
    56701 attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa tggcgatacg
    56761 atagccttta atcgccgcct tcacgccgcc gccgcctggt agcgccacgc tttccatcat
    56821 atgcaccaca gtgcggacaa tggatcccgg gaagacacgt tcaccgggaa ccggggggtc
    56881 aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg
    56941 cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat
    57001 gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt
    57061 ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc
    57121 taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa
    57181 ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag gaatggtatt
    57241 gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg gtcattacca ccatcggttt
    57301 aaccgttgag cccggttcaa acacgtcggt gatggtacgg ttacgcatcg cctctttcgg
    57361 cgtgccgctc agattgttag ggttgtatga cgggctgtta gccatcgcca gcacttcacc
    57421 ggtgttgaca tccaccagca cggcgctacc agattcagcc ttgttaaagg ccaccgcgtt
    57481 gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg ccaggttgtg
    57541 cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg cgaccatagc ggtctttacg
    57601 cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct caacgccctc
    57661 aatcccttga ctatcgacgt tagtaaagcc gatgaggtga gcagtcactt cgccggacgg
    57721 atagtaacgg cgagactctt cacgcagatg aatccccggc agtttcagtt ttttgatgta
    57781 gtccgccatg tcagggttca cctgacgcgc cagataaata aaacgccctt tcgggttggc
    57841 gttaatgcgg gctgaaagct gatccagcgg aatattgagc gcgttagcca gcgccttcca
    57901 gcggtcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc
    57961 ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga
    58021 ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tctttcacca gcatatccgg
    58081 ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca
    58141 gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct gacgttttgg
    58201 tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat
    58261 attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga tcctttccac
    58321 ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa
    58381 agcatctcgc tccagcacca gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc
    58441 cgtggttacc acagtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt
    58501 cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct cgtggcttcc
    58561 catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc attcgtcctc
    58621 tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc ttcttcgccc
    58681 ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag ctgctcttca
    58741 gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc
    58801 acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc cggggccagc
    58861 acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg
    58921 cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac cggcgttgca
    58981 acagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg
    59041 acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccaa
    59101 gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg
    59161 tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgctc agcatcatca
    59221 agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc gataagatcg
    59281 cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga
    59341 tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc
    59401 ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca
    59461 tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc
    59521 gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc gctccgataa
    59581 gtctccggta gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt
    59641 ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc
    59701 ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc
    59761 gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca tgctcgacag
    59821 acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca gcaggcacgg
    59881 gtgatgaatg tcaatggtgc aaaccatttg accgacagcg ttctcaagca gctgttcccg
    59941 gtaacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg
    60001 gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt
    60061 ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc
    60121 gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgca gcaaattaac
    60181 gttatgaata gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa atgactcact
    60241 attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc
    60301 ttccccggtt aacagtccct gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca
    60361 aaagcaaaga aaagtgcttg ttaaatcggc aattcatctc tgcatgtaac aaaataatgc
    60421 aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc agctggtgag ttaattaacg
    60481 ggcgggttta aaagaactta tcaataatag tcaggaatag tcttatttac tttaaggata
    60541 ttgatgtcca ttcccgtact ctacg
//
LOCUS       DAJTXQ010000006        59841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000006 DAJTXQ010000000
VERSION     DAJTXQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.006"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..262)
                     /locus_tag="OO515_000359"
     CDS             complement(<1..262)
                     /locus_tag="OO515_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6574385.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OO515_000360"
     CDS             369..665
                     /locus_tag="OO515_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6574386.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO515_000361"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO515_000361"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6574387.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO515_000362"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO515_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6574388.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO515_000363"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO515_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6574389.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OO515_000364"
     CDS             complement(3472..3645)
                     /locus_tag="OO515_000364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574390.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OO515_000365"
     CDS             3673..3756
                     /locus_tag="OO515_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6574391.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO515_000366"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO515_000366"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6574392.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO515_000367"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO515_000367"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6574393.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OO515_000368"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OO515_000368"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6574394.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO515_000369"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO515_000369"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6574395.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO515_000370"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO515_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6574396.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO515_000371"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO515_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6574397.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OO515_000372"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OO515_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6574398.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO515_000373"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO515_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6574399.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OO515_000374"
     CDS             14052..15254
                     /locus_tag="OO515_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6574400.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OO515_000375"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OO515_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OO515_000376"
     CDS             16953..18560
                     /locus_tag="OO515_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6574401.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OO515_000377"
     CDS             18822..20444
                     /locus_tag="OO515_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6574402.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OO515_000378"
     CDS             20810..21877
                     /locus_tag="OO515_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6574403.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OO515_000379"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OO515_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6574404.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OO515_000380"
     CDS             24609..25733
                     /locus_tag="OO515_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6574405.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OO515_000381"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OO515_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OO515_000382"
     CDS             26076..26435
                     /locus_tag="OO515_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6574406.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO515_000383"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO515_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6574407.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO515_000384"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO515_000384"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6574408.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO515_000385"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO515_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6574409.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO515_000386"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO515_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6574410.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO515_000387"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO515_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6574411.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO515_000388"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO515_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6574412.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO515_000389"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO515_000389"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6574413.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OO515_000390"
     CDS             complement(32630..33679)
                     /locus_tag="OO515_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6574414.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OO515_000391"
     CDS             33811..35028
                     /locus_tag="OO515_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6574415.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO515_000392"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO515_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6574416.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OO515_000393"
     CDS             complement(35662..36327)
                     /locus_tag="OO515_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6574417.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OO515_000394"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OO515_000394"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6574418.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO515_000395"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO515_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6574419.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OO515_000396"
     CDS             complement(39167..39793)
                     /locus_tag="OO515_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6574420.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OO515_000397"
     CDS             39934..40293
                     /locus_tag="OO515_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6574421.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OO515_000398"
     CDS             complement(40296..40565)
                     /locus_tag="OO515_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6574422.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO515_000399"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO515_000399"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6574423.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OO515_000400"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OO515_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6574424.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OO515_000401"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OO515_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6574425.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OO515_000402"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OO515_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6574426.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OO515_000403"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OO515_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6574427.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OO515_000404"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OO515_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6574428.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO515_000405"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO515_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6574429.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OO515_000406"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OO515_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6574430.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OO515_000407"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OO515_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6574431.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OO515_000408"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OO515_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6574432.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OO515_000409"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OO515_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6574433.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OO515_000410"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OO515_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6574434.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OO515_000411"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OO515_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OO515_000412"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OO515_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6574435.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OO515_000413"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OO515_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6574436.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="OO515_000414"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="OO515_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6574437.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO515_000415"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO515_000415"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6574438.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="OO515_000416"
     CDS             complement(57808..58248)
                     /locus_tag="OO515_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6574439.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59841
                     /gene="ggt"
                     /locus_tag="OO515_000417"
     CDS             58368..>59841
                     /gene="ggt"
                     /locus_tag="OO515_000417"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6574440.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14593 a        15113 c        16140 g        13995 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataag gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtgg
    59581 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    59641 ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg aacatttacg
    59701 ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcatcgatgt
    59761 caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt cctggcggga
    59821 gtcggattat taccaccgtg c
//
LOCUS       DAJTXQ010000007        58119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000007 DAJTXQ010000000
VERSION     DAJTXQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.007"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..161
                     /locus_tag="OO515_000418"
     CDS             <1..161
                     /locus_tag="OO515_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6574441.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="OO515_000419"
     CDS             complement(199..1260)
                     /locus_tag="OO515_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6574442.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="OO515_000420"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="OO515_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6574443.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="OO515_000421"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="OO515_000421"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6574444.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO515_000422"
     CDS             complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO515_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6574445.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO515_000423"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO515_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6574446.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO515_000424"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO515_000424"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6574447.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="OO515_000425"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="OO515_000425"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6574448.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="OO515_000426"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="OO515_000426"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6574449.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="OO515_000427"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="OO515_000427"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6574450.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="OO515_000428"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="OO515_000428"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6574451.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="OO515_000429"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="OO515_000429"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6574452.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="OO515_000430"
     CDS             complement(14498..14650)
                     /locus_tag="OO515_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574453.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="OO515_000431"
     CDS             14668..14859
                     /locus_tag="OO515_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6574454.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15170..15688
                     /locus_tag="OO515_000432"
     CDS             15170..15688
                     /locus_tag="OO515_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6574455.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15704..16243
                     /locus_tag="OO515_000433"
     CDS             15704..16243
                     /locus_tag="OO515_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6574456.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="OO515_000434"
     CDS             complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="OO515_000434"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6574457.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="OO515_000435"
     CDS             complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="OO515_000435"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6574458.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19612..20982
                     /gene="xseA"
                     /locus_tag="OO515_000436"
     CDS             19612..20982
                     /gene="xseA"
                     /locus_tag="OO515_000436"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6574459.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20979..21194)
                     /locus_tag="OO515_000437"
     CDS             complement(20979..21194)
                     /locus_tag="OO515_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6574460.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21263..22735)
                     /gene="der"
                     /locus_tag="OO515_000438"
     CDS             complement(21263..22735)
                     /gene="der"
                     /locus_tag="OO515_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6574461.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="OO515_000439"
     CDS             complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="OO515_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6574462.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="OO515_000440"
     CDS             complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="OO515_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6574463.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="OO515_000441"
     CDS             complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="OO515_000441"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6574464.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="OO515_000442"
                     /gene_synonym="gcpE"
     CDS             complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="OO515_000442"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6574465.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="OO515_000443"
     CDS             complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="OO515_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6574466.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28508..29662)
                     /gene="trmG/rlmN"
                     /locus_tag="OO515_000444"
     CDS             complement(28508..29662)
                     /gene="trmG/rlmN"
                     /locus_tag="OO515_000444"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6574467.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="OO515_000445"
     CDS             complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="OO515_000445"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6574468.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="OO515_000446"
     CDS             complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="OO515_000446"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6574469.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="OO515_000447"
     CDS             complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="OO515_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6574470.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37873..38718
                     /gene="sseA"
                     /locus_tag="OO515_000448"
     CDS             37873..38718
                     /gene="sseA"
                     /locus_tag="OO515_000448"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6574471.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="OO515_000449"
     CDS             complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="OO515_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6574472.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="OO515_000450"
     CDS             complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="OO515_000450"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6574473.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="OO515_000451"
     CDS             complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="OO515_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6574474.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="OO515_000452"
     CDS             complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="OO515_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6574475.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="OO515_000453"
     CDS             complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="OO515_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6574476.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="OO515_000454"
     CDS             complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="OO515_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6574477.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="OO515_000455"
     CDS             complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="OO515_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6574478.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="OO515_000456"
     CDS             complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="OO515_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6574479.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="OO515_000457"
     CDS             complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="OO515_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6574480.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="OO515_000458"
     CDS             complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="OO515_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6574481.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="OO515_000459"
     CDS             complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="OO515_000459"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6574482.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48471..49274
                     /gene="suhB"
                     /locus_tag="OO515_000460"
     CDS             48471..49274
                     /gene="suhB"
                     /locus_tag="OO515_000460"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6574483.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49419..50273
                     /locus_tag="OO515_000461"
     CDS             49419..50273
                     /locus_tag="OO515_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6574484.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50464..51744
                     /gene="csiE"
                     /locus_tag="OO515_000462"
     CDS             50464..51744
                     /gene="csiE"
                     /locus_tag="OO515_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6574485.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51736..52875)
                     /locus_tag="OO515_000463"
     CDS             complement(51736..52875)
                     /locus_tag="OO515_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6574486.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="OO515_000464"
                     /pseudo
     CDS             complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="OO515_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54061..55422
                     /locus_tag="OO515_000465"
     CDS             54061..55422
                     /locus_tag="OO515_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6574487.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55419..55937
                     /locus_tag="OO515_000466"
     CDS             55419..55937
                     /locus_tag="OO515_000466"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6574488.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55937..56257
                     /locus_tag="OO515_000467"
     CDS             55937..56257
                     /locus_tag="OO515_000467"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6574489.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56254..57066
                     /gene="hcaB"
                     /locus_tag="OO515_000468"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56254..57066
                     /gene="hcaB"
                     /locus_tag="OO515_000468"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6574490.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57076..>58119
                     /locus_tag="OO515_000469"
     CDS             57076..>58119
                     /locus_tag="OO515_000469"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6574491.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLPLLPPPWFWSDQYSDNLQFIG
                     DMRGDDWLCRGNPE"
BASE COUNT        14193 a        15733 c        14494 g        13699 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgtggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
    15001 gcggaataaa tataaaatta atatatatgt tgtaatgata tatttttata aattattccc
    15061 tgcgtgaatt ttaataaatt taatctatcc ctttatacgc aatacattta ctttcctctt
    15121 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
    15181 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
    15241 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
    15301 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
    15361 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
    15421 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
    15481 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
    15541 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca
    15601 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
    15661 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
    15721 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
    15781 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
    15841 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
    15901 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
    15961 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
    16021 cagcttagag attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg
    16081 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
    16141 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
    16201 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
    16261 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
    16321 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
    16381 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
    16441 aaatcgtacg gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
    16501 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
    16561 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
    16621 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
    16681 acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg
    16741 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
    16801 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
    16861 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
    16921 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga
    16981 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
    17041 tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc
    17101 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
    17161 ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg
    17221 aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt
    17281 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
    17341 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
    17401 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
    17461 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
    17521 tctgcggtca gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc
    17581 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
    17641 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
    17701 ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg
    17761 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
    17821 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtctaga
    17881 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
    17941 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
    18001 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
    18061 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
    18121 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
    18181 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
    18241 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
    18301 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
    18361 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
    18421 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
    18481 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
    18541 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
    18601 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
    18661 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
    18721 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
    18781 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
    18841 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
    18901 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
    18961 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
    19021 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
    19081 cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg
    19141 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
    19201 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
    19261 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
    19321 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
    19381 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
    19441 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
    19501 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
    19561 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
    19621 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
    19681 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
    19741 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
    19801 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
    19861 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
    19921 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
    19981 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
    20041 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
    20101 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
    20161 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
    20221 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
    20281 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
    20341 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
    20401 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
    20461 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
    20521 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
    20581 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
    20641 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
    20701 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
    20761 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
    20821 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
    20881 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
    20941 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
    21001 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
    21061 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
    21121 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
    21181 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
    21241 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
    21301 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
    21361 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
    21421 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
    21481 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
    21541 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
    21601 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
    21661 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
    21721 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
    21781 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
    21841 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggtatcttca attgcctgca
    21901 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
    21961 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
    22021 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
    22081 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
    22141 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
    22201 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
    22261 gcacacgctc cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt
    22321 cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
    22381 tgtttgccac caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat
    22441 ctgccggcat caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
    22501 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
    22561 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
    22621 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
    22681 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
    22741 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
    22801 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
    22861 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
    22921 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
    22981 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
    23041 ccggcgaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
    23101 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
    23161 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
    23221 caagcgccgt caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg
    23281 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
    23341 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
    23401 cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
    23461 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta
    23521 actgaccgtt actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg
    23581 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
    23641 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa
    23701 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
    23761 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
    23821 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
    23881 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
    23941 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
    24001 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
    24061 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
    24121 catgcactac gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg
    24181 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
    24241 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
    24301 tcatcactcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
    24361 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
    24421 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
    24481 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
    24541 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
    24601 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
    24661 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
    24721 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
    24781 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
    24841 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
    24901 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
    24961 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
    25021 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
    25081 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca
    25141 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    25201 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    25261 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    25321 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    25381 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    25441 ccagcgaacc aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc
    25501 accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    25561 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    25621 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    25681 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    25741 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    25801 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    25861 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    25921 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    25981 tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga
    26041 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    26101 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    26161 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    26221 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    26281 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    26341 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga
    26401 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    26461 cgccagcgat acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat
    26521 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    26581 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    26641 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat
    26701 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    26761 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    26821 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    26881 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    26941 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    27001 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    27061 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    27121 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    27181 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    27241 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    27301 tactggatct gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt
    27361 aaattcaaat taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca
    27421 gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    27481 ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct
    27541 gccgttggta ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    27601 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    27661 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    27721 gtatttaacg ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga
    27781 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    27841 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt
    27901 tttttgcggc gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt
    27961 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    28021 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    28081 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    28141 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    28201 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    28261 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    28321 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    28381 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    28441 gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta
    28501 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    28561 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg atatcatcac
    28621 cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    28681 cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca
    28741 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    28801 cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg
    28861 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    28921 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    28981 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    29041 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    29101 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc
    29161 gctgaccggt gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa
    29221 tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    29281 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    29341 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    29401 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    29461 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    29521 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    29581 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    29641 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    29701 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    29761 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    29821 gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac
    29881 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    29941 accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    30001 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    30061 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag
    30121 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    30181 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    30241 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    30301 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    30361 gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga
    30421 tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    30481 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    30541 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    30601 tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg
    30661 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    30721 gttccagcgg tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg
    30781 cgacgcgctg ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct
    30841 gctctgacag taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc
    30901 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    30961 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    31021 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    31081 tgccgtcagg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    31141 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    31201 aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg
    31261 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    31321 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    31381 cagcgccgcc gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca
    31441 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    31501 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    31561 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    31621 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    31681 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    31741 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    31801 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    31861 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    31921 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    31981 cccttgactc ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca
    32041 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    32101 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    32161 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    32221 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    32281 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    32341 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    32401 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    32461 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    32521 tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg
    32581 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    32641 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    32701 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    32761 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    32821 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    32881 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca
    32941 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt
    33001 ccagacccac aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    33061 cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    33121 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    33181 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca
    33241 cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca
    33301 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    33361 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    33421 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    33481 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    33541 gctcatcact atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg
    33601 catcacccat ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat
    33661 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    33721 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    33781 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    33841 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    33901 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    33961 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    34021 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    34081 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    34141 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    34201 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    34261 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    34321 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    34381 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    34441 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    34501 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    34561 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    34621 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    34681 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    34741 ttaccgacag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    34801 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    34861 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    34921 gccacggatc tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca
    34981 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    35041 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    35101 tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    35161 atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt
    35221 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    35281 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat
    35341 gcaacttaat ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt
    35401 cgggtcgcac tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac
    35461 gcacgctact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    35521 ccgggaaact gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga
    35581 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    35641 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    35701 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    35761 tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc
    35821 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    35881 ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt tccttccact
    35941 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    36001 gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    36061 cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggcg
    36121 caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc gtaagcgggg
    36181 ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg acgtggaaat
    36241 cccacatccg gtactggtta tcgcccgtgt tggcgcgaat atgccacata ccggttgccg
    36301 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgt
    36361 tcctgagtac ctgcccgtca ggtttaatca cgtctaactt gatgggttga tcgggcaatg
    36421 ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac cgtttcacct gggcgataaa
    36481 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    36541 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    36601 gtgccagcag taatgccgca tttttatcat tttccagctg cacatgcccc tgagcgtcac
    36661 tggttgcctg agtcagagtc tgtcctttct catttaataa agagacttca attccttgct
    36721 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    36781 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    36841 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    36901 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    36961 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    37021 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    37081 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    37141 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    37201 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    37261 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    37321 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    37381 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    37441 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    37501 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    37561 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    37621 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    37681 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    37741 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    37801 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    37861 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    37921 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    37981 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    38041 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    38101 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    38161 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    38221 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    38281 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    38341 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    38401 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    38461 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    38521 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    38581 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    38641 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    38701 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    38761 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    38821 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    38881 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    38941 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    39001 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    39061 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    39121 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    39181 cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt taaagtcacg
    39241 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    39301 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    39361 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    39421 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    39481 cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    39541 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    39601 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    39661 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    39721 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    39781 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    39841 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    39901 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    39961 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    40021 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    40081 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    40141 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    40201 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    40261 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    40321 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    40381 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    40441 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    40501 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    40561 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    40621 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    40681 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    40741 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    40801 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ctggcgcttc
    40861 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    40921 tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    40981 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    41041 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    41101 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    41161 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    41221 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    41281 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    41341 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    41401 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    41461 tttttccgct aaatgattcg cgttgcagaa aggcggcaag tgagtgaagc cccaggagca
    41521 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    41581 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    41641 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    41701 ggtgaatcga accgttttcg gatcaagatc aggatacgca tcgtacagtg cttcgccaat
    41761 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    41821 ttgatagtgt aacgcgggat ttcgactact aaatcttcgt cggtaacacg tgcctggcag
    41881 cttaaacggc tttcgggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    41941 gagctttctg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    42001 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    42061 gtttcaccgc tattagcttc cagaacagcg ccatcaggac agagatcctg atgaggcaaa
    42121 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    42181 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    42241 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    42301 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    42361 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    42421 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    42481 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    42541 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    42601 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    42661 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    42721 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    42781 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    42841 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    42901 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    42961 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt cagcttctac
    43021 acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    43081 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    43141 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    43201 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    43261 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    43321 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    43381 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    43441 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    43501 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    43561 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    43621 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    43681 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    43741 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    43801 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    43861 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    43921 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    43981 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    44041 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    44101 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    44161 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    44221 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    44281 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    44341 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    44401 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    44461 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagaaggtg aagtaatcca
    44521 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    44581 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    44641 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    44701 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    44761 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    44821 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    44881 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    44941 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    45001 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    45061 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    45121 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    45181 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    45241 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    45301 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    45361 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    45421 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    45481 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    45541 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    45601 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    45661 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    45721 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    45781 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    45841 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    45901 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    45961 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    46021 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    46081 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    46141 aaacaaggat ggtgtcatca cgcatcgctg cttcaagttc tttcagatcg ataatgccgt
    46201 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    46261 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    46321 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    46381 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    46441 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    46501 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    46561 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    46621 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    46681 ttctaatatc ttgttatctt taacctaact gtttcgggtt tcagcaagtt gacaaactta
    46741 cgaatctccg ggagcttaca taagtaagtg accggagtga gtatgtgcag tcaacgcaga
    46801 tgcaacacga aacaggacgg ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta
    46861 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    46921 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    46981 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcaaccgcct ttaccctgac
    47041 aacgggtagc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    47101 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    47161 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    47221 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    47281 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    47341 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    47401 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    47461 ccattttttt atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg
    47521 caattattcg cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg
    47581 aggggaccgt ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct
    47641 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    47701 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    47761 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    47821 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    47881 tacgcacttc ataggcaata acctgaaccg ccattgccaa gttcagcgaa ctgtattccg
    47941 ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga
    48001 cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca
    48061 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc
    48121 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    48181 caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg
    48241 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    48301 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    48361 ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    48421 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    48481 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg
    48541 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag
    48601 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttactca cagcacacca
    48661 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    48721 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    48781 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    48841 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca
    48901 ccgctcgcga tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt
    48961 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    49021 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    49081 ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcag
    49141 gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt aacatcgttg
    49201 ctggtaaccc gcgcgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    49261 ctctgaagcg ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg
    49321 ctattctgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    49381 ccgattaatg gatgataaag aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac
    49441 gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    49501 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    49561 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    49621 cttcttcgac gggccctgct gacaatgcca tcgcaaccat cattcatgct cacggcaatg
    49681 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    49741 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    49801 tgctggacga tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    49861 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    49921 aggatgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa
    49981 ccatcgccaa tcagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    50041 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    50101 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt
    50161 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt
    50221 atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    50281 tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa
    50341 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    50401 ttcgccattt ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    50461 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    50521 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    50581 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    50641 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    50701 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    50761 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    50821 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    50881 ctgcaaaaac gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    50941 ttgctgcaac accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    51001 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    51061 ctccagcctg taccgcctga tgagccactg tttatggcat tacttttttc catgttgcgg
    51121 gttcccgatc cattgcgtga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa
    51181 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    51241 tgcgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    51301 attgataata ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc
    51361 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc
    51421 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    51481 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta
    51541 cgcgaactaa cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag
    51601 acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc
    51661 ttttcaccac cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc
    51721 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    51781 cgccaccagc gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    51841 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    51901 acggatgact tcgctaccct ggcgaacagc aatatagcgc atggcggcca ggtggcagac
    51961 cgtgaaggtg ccgcaatgca aaatttgcac cactatcaac cacggcaacg ccgtagttgc
    52021 tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgagc
    52081 actacaacgg cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc
    52141 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    52201 gctaaaaccg taataggccg catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    52261 ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact
    52321 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    52381 caacgtcaac agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    52441 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    52501 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    52561 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    52621 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg
    52681 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    52741 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    52801 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    52861 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt
    52921 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    52981 cacaagcact aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc
    53041 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    53101 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    53161 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    53221 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    53281 ctggctgttt tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    53341 cggatcggta ctgacgaaat tcacgccatc cagttacgcg gcggtgatct ctttttcatg
    53401 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    53461 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    53521 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    53581 tctgagacga aacattggta atacttttgg caataaattc acttccgccg atggcacaaa
    53641 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    53701 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    53761 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    53821 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    53881 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    53941 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccaac
    54001 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    54061 atgaccacac cctcagattt aaacatttac caactgattg atacccaaaa tggtcgggtc
    54121 actccgcgta tttataccga cccagatatt taccaactgg agcttgaacg tattttcggc
    54181 cgttgctggt tatttctcgc ccacgaaagc cagatcccaa aacctggtga tttctttaac
    54241 acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg
    54301 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    54361 cgcgccttta cctgtccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    54421 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac
    54481 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    54541 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    54601 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg
    54661 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    54721 gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa
    54781 accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac
    54841 ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt
    54901 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc
    54961 ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc
    55021 acgctccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    55081 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    55141 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    55201 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    55261 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc
    55321 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    55381 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    55441 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    55501 aatttcgtga ctggctggag cagctcgaca aagagattcg ttacaccatg cgcaccacag
    55561 ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta
    55621 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg
    55681 cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata agcgaaaccg
    55741 acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc
    55801 gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca
    55861 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    55921 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    55981 tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt
    56041 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    56101 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    56161 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    56221 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    56281 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    56341 ggcgcgcaag ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga
    56401 tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc
    56461 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcaggc
    56521 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    56581 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg
    56641 gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc caggtatcct
    56701 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa
    56761 ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc
    56821 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg
    56881 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt
    56941 acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    57001 atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat
    57061 ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg
    57121 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    57181 gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat
    57241 tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg
    57301 cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac
    57361 ggcgaaagct ggcactggga tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg
    57421 ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc
    57481 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    57541 ggtctggaac tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg
    57601 gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc
    57661 caccagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt
    57721 gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac
    57781 ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat
    57841 ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat
    57901 gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc
    57961 aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgctacc gctaccgcta
    58021 ctgccgccgc cgtggttctg gagcgatcag tacagtgata acttacagtt tattggcgat
    58081 atgcgtggcg atgactggct ttgtcgtggc aacccggaa
//
LOCUS       DAJTXQ010000008        57429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000008 DAJTXQ010000000
VERSION     DAJTXQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.008"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            144..1487
                     /gene="argG"
                     /locus_tag="OO515_000470"
     CDS             144..1487
                     /gene="argG"
                     /locus_tag="OO515_000470"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6574492.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1495..3102)
                     /locus_tag="OO515_000471"
     CDS             complement(1495..3102)
                     /locus_tag="OO515_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6574493.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="OO515_000472"
     tRNA            complement(3580..3666)
                     /locus_tag="OO515_000472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="OO515_000473"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="OO515_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6574494.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="OO515_000474"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="OO515_000474"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6574495.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="OO515_000475"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="OO515_000475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6574496.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="OO515_000476"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="OO515_000476"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6574497.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="OO515_000477"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="OO515_000477"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6574498.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="OO515_000478"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="OO515_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6574499.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="OO515_000479"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="OO515_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6574500.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="OO515_000480"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="OO515_000480"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6574501.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="OO515_000481"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="OO515_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6574502.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="OO515_000482"
     CDS             complement(13278..14243)
                     /locus_tag="OO515_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6574503.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="OO515_000483"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="OO515_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6574504.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="OO515_000484"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="OO515_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6574505.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="OO515_000485"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="OO515_000485"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6574506.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16418..16696
                     /gene="sfsB"
                     /locus_tag="OO515_000486"
     CDS             16418..16696
                     /gene="sfsB"
                     /locus_tag="OO515_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6574507.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16744..18003)
                     /gene="murA"
                     /locus_tag="OO515_000487"
     CDS             complement(16744..18003)
                     /gene="murA"
                     /locus_tag="OO515_000487"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6574508.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18058..18312)
                     /gene="ibaG"
                     /locus_tag="OO515_000488"
     CDS             complement(18058..18312)
                     /gene="ibaG"
                     /locus_tag="OO515_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6574509.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18472..18765)
                     /gene="mlaB"
                     /locus_tag="OO515_000489"
     CDS             complement(18472..18765)
                     /gene="mlaB"
                     /locus_tag="OO515_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6574510.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18765..19400)
                     /gene="mlaC"
                     /locus_tag="OO515_000490"
     CDS             complement(18765..19400)
                     /gene="mlaC"
                     /locus_tag="OO515_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6574511.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19419..19970)
                     /gene="mlaD"
                     /locus_tag="OO515_000491"
     CDS             complement(19419..19970)
                     /gene="mlaD"
                     /locus_tag="OO515_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6574512.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19975..20757)
                     /gene="mlaE"
                     /locus_tag="OO515_000492"
     CDS             complement(19975..20757)
                     /gene="mlaE"
                     /locus_tag="OO515_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6574513.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20765..21574)
                     /gene="mlaF"
                     /locus_tag="OO515_000493"
     CDS             complement(20765..21574)
                     /gene="mlaF"
                     /locus_tag="OO515_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6574514.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21784..22761
                     /locus_tag="OO515_000494"
     CDS             21784..22761
                     /locus_tag="OO515_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6574515.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22775..23761
                     /gene="kdsD"
                     /locus_tag="OO515_000495"
     CDS             22775..23761
                     /gene="kdsD"
                     /locus_tag="OO515_000495"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6574516.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23782..24348
                     /gene="kdsC"
                     /locus_tag="OO515_000496"
     CDS             23782..24348
                     /gene="kdsC"
                     /locus_tag="OO515_000496"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6574517.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24345..24920
                     /gene="lptC"
                     /locus_tag="OO515_000497"
     CDS             24345..24920
                     /gene="lptC"
                     /locus_tag="OO515_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6574518.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24889..25446
                     /gene="lptA"
                     /locus_tag="OO515_000498"
     CDS             24889..25446
                     /gene="lptA"
                     /locus_tag="OO515_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6574519.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25453..26178
                     /gene="lptB"
                     /locus_tag="OO515_000499"
     CDS             25453..26178
                     /gene="lptB"
                     /locus_tag="OO515_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6574520.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26226..27659
                     /gene="rpoN"
                     /locus_tag="OO515_000500"
     CDS             26226..27659
                     /gene="rpoN"
                     /locus_tag="OO515_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6574521.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27682..27969
                     /gene="hpf"
                     /locus_tag="OO515_000501"
     CDS             27682..27969
                     /gene="hpf"
                     /locus_tag="OO515_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6574522.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28087..28578
                     /gene="ptsN"
                     /locus_tag="OO515_000502"
     CDS             28087..28578
                     /gene="ptsN"
                     /locus_tag="OO515_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6574523.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28624..29478
                     /gene="rapZ"
                     /locus_tag="OO515_000503"
     CDS             28624..29478
                     /gene="rapZ"
                     /locus_tag="OO515_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6574524.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29475..29747
                     /gene="npr"
                     /locus_tag="OO515_000504"
     CDS             29475..29747
                     /gene="npr"
                     /locus_tag="OO515_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6574525.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29961..30593
                     /gene="yrbL"
                     /locus_tag="OO515_000505"
     CDS             29961..30593
                     /gene="yrbL"
                     /locus_tag="OO515_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6574526.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30590..31318)
                     /gene="mtgA"
                     /locus_tag="OO515_000506"
     CDS             complement(30590..31318)
                     /gene="mtgA"
                     /locus_tag="OO515_000506"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6574527.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31315..31968)
                     /gene="elbB"
                     /locus_tag="OO515_000507"
     CDS             complement(31315..31968)
                     /gene="elbB"
                     /locus_tag="OO515_000507"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6574528.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32198..34534)
                     /gene="arcB"
                     /locus_tag="OO515_000508"
     CDS             complement(32198..34534)
                     /gene="arcB"
                     /locus_tag="OO515_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6574529.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDIAVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34630..35559)
                     /locus_tag="OO515_000509"
     CDS             complement(34630..35559)
                     /locus_tag="OO515_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6574530.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36234..40694
                     /gene="gltB"
                     /locus_tag="OO515_000510"
     CDS             36234..40694
                     /gene="gltB"
                     /locus_tag="OO515_000510"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6574531.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40707..42125
                     /gene="gltD"
                     /locus_tag="OO515_000511"
     CDS             40707..42125
                     /gene="gltD"
                     /locus_tag="OO515_000511"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6574532.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42309..42788
                     /locus_tag="OO515_000512"
     CDS             42309..42788
                     /locus_tag="OO515_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6574533.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42805..43110
                     /locus_tag="OO515_000513"
     CDS             42805..43110
                     /locus_tag="OO515_000513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6574534.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43186..43650)
                     /locus_tag="OO515_000514"
     CDS             complement(43186..43650)
                     /locus_tag="OO515_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6574535.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43647..44522)
                     /gene="nanK"
                     /locus_tag="OO515_000515"
     CDS             complement(43647..44522)
                     /gene="nanK"
                     /locus_tag="OO515_000515"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6574536.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44519..45208)
                     /locus_tag="OO515_000516"
     CDS             complement(44519..45208)
                     /locus_tag="OO515_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6574537.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45256..46746)
                     /gene="nanT"
                     /locus_tag="OO515_000517"
     CDS             complement(45256..46746)
                     /gene="nanT"
                     /locus_tag="OO515_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6574538.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46856..47749)
                     /gene="nanA"
                     /locus_tag="OO515_000518"
     CDS             complement(46856..47749)
                     /gene="nanA"
                     /locus_tag="OO515_000518"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6574539.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47871..48662)
                     /gene="nanR"
                     /locus_tag="OO515_000519"
     CDS             complement(47871..48662)
                     /gene="nanR"
                     /locus_tag="OO515_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6574540.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49042..50364
                     /gene="dcuC"
                     /locus_tag="OO515_000520"
     CDS             49042..50364
                     /gene="dcuC"
                     /locus_tag="OO515_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6574541.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50407..50904)
                     /gene="sspB"
                     /locus_tag="OO515_000521"
     CDS             complement(50407..50904)
                     /gene="sspB"
                     /locus_tag="OO515_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6574542.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50910..51548)
                     /gene="sspA"
                     /locus_tag="OO515_000522"
     CDS             complement(50910..51548)
                     /gene="sspA"
                     /locus_tag="OO515_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6574543.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51943..52335)
                     /gene="rpsI"
                     /locus_tag="OO515_000523"
     CDS             complement(51943..52335)
                     /gene="rpsI"
                     /locus_tag="OO515_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6574544.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52351..52779)
                     /gene="rplM"
                     /locus_tag="OO515_000524"
     CDS             complement(52351..52779)
                     /gene="rplM"
                     /locus_tag="OO515_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6574545.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52998..54125)
                     /gene="zapE"
                     /locus_tag="OO515_000525"
     CDS             complement(52998..54125)
                     /gene="zapE"
                     /locus_tag="OO515_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6574546.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54319..54717
                     /locus_tag="OO515_000526"
     CDS             54319..54717
                     /locus_tag="OO515_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6574547.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54871..56238
                     /gene="degQ"
                     /locus_tag="OO515_000527"
     CDS             54871..56238
                     /gene="degQ"
                     /locus_tag="OO515_000527"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6574548.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56328..57395
                     /gene="degS"
                     /locus_tag="OO515_000528"
     CDS             56328..57395
                     /gene="degS"
                     /locus_tag="OO515_000528"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6574549.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14263 a        15303 c        14254 g        13609 t
ORIGIN      
        1 cgcattattt tacaaaaaac acactaaact cttcctgtct ccgataaaag atgattaaat
       61 gaaaactcat ttattttgca taaaaattca gtgagagcgg aaatccaggc tcatcatcag
      121 ttaattaagc agggtgttat tttatgacga cgattctcaa gcatctcccg gtaggtcaac
      181 gtattggtat cgctttttcc ggcggtctgg acaccagtgc cgcactgctg tggatgcgac
      241 aaaagggagc ggttccttat gcatatactg caaacctggg ccagccagac gaagaggatt
      301 atgatgcgat ccctcgtcgt gccatggaat acggcgcgga gaacgcacgt ctgatcgact
      361 gccgcaaaca actggtggcc gaaggtattg ccgctattca gtgtggcgca tttcataaca
      421 ccaccggcgg cctgacctat ttcaacacga cgccgctggg ccgcgccgtg actggtacca
      481 tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg gggtgacggt agcacctata
      541 aaggaaacga tatcgaacgt ttctatcgtt atggtctgct gaccaatgct gaactgcaga
      601 tttacaaacc gtggcttgat actgacttta ttgatgaact gggcggccgt catgagatgt
      661 ctgaatttat gattgcctgc ggtttcgact acaaaatgtc tgtcgaaaaa gcctactcca
      721 cagactccaa catgcttggt gcaacgcacg aagcgaagga tctggaatac ctcaactcca
      781 gcgtcaaaat cgtcaaccca attatgggcg tgaaattctg ggatgagagc gtgaagatcc
      841 cggcagaaga agtcacagta cgctttgagc aaggtcatcc ggtagcgctg aacggtaaaa
      901 cctttagcga cgacgtagaa atgatgctgg aagctaaccg catcggcggt cgtcacggcc
      961 tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc gaaaagccgt ggtatttacg
     1021 aagctccggg gatggcactg ctacatatcg cttatgaacg tctgttgacc ggtattcaca
     1081 acgaagacac cattgagcag tatcatgcac atggtcgtca gttgggccgt ctgctgtacc
     1141 aggggcgttg gtttgattcc caggcgctga tgctgcgtga ctctctgcaa cgctgggttg
     1201 ccagccagat cactggtgaa gttaccctgg agctgcgccg tgggaacgat tattcaatcc
     1261 tgaataccgt ctcagagaac ctgacctaca agccagagcg tctgacgatg gaaaaaggcg
     1321 actcggtgtt ctcgccagat gatcgtattg gtcaattgac catgcgtaac ctggatatca
     1381 ctgatacccg agagaaactt tttggttatg ccaaaactgg cctgctctcc tcctctgcca
     1441 cttcaggcgt gccgcaggtg gagaatctgg aaaacaaagg ccagtaattc gacatcaacc
     1501 ctgcccttca ggggtggggt taccttgctt atcctctgta aactcctttc tgagcatcac
     1561 ataatcgaaa acatcatggt ttgcatccac aacccgtgag tctcctttat agtgtgcaat
     1621 cacttcctcc agcgttttcg gttgttccgg ctttgttacc cccatccacg cattaataag
     1681 gtaattattg taagctgtcg aaaagatgtt gttttccgtt gtacgatcca cgccatcccc
     1741 aagaacgggg ctataccaga taaacatcgg gacatgatat gcctgctggc tagcctccct
     1801 gcctccatga aaatagacgt tcttcttcgt agggtcacgt tccaggccgt ggtcggcaaa
     1861 atacatgacc gaggcgcggc gatcttttaa taattcaaaa acctgaccta gcaaactatc
     1921 tgtgtaatga atggagttgt catagcaggc atcctgatcg tcctgcggtt gaaacacggc
     1981 gctggattgc ggataggcgc tacaagccgg ttcatggctt ccatttaaat gaagaacaat
     2041 cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat aaatgcggca acaacaattc
     2101 atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg gcgatactgg taactgctgt
     2161 accattctgc cgaaaagcgg attgcgagct tagccagaaa gtctgaaatc ctgcttgatt
     2221 agccatatta ataatgttgt cggggtaatt atgaatgtca tgactcaaaa cagaatcagc
     2281 agttaaagaa aggggaaccg acagcgcagt gtaaggtgcg ccgcttattg attgattaaa
     2341 cagtttgatc tgttttcttt gtgcttcaac ttgcggtgtc gtagagcgtg tatatccata
     2401 caaagacata ttgtcgacac gtacagactc ccccacaatc aacacgtagg tatcaatacc
     2461 tgtatccctg actgataatt gaaaatacgg caccgtgttt gcaattgaga gtaatctttg
     2521 atgctctttc gctgctaaag caaaataatt gagattgaaa aacggcgtat aggtagcaaa
     2581 tcgcgacgct agtatatatg gactgaatgc ttttttattt tttgcatctt tataagcaaa
     2641 ttgacaagca gaaaataaac tgcccgaaat gacaatcagc aataatattc ctgtcacttt
     2701 ttttgtcggc aaggaaacat catattttat tattactgcc aaaaaaagaa gggataaaaa
     2761 agcaaaagca catagataag ggatatacat ccccagcatt ttgacaactt catccggatc
     2821 actctgcagc acactaatcg caaatccatc attgaaagtt gtaccaaagg tacaccatga
     2881 atagaggcta atactcatgt ctgccgcagt cagtaacaca aatggtgcgg caacaagcag
     2941 gcgaagcgga agggatgata tacgttttac aaccaggatg gtcaggacga agaacagcaa
     3001 tcgctgaacc atatgcgcgc cggaagaggc gactaagttc gtaataccaa aaacaataac
     3061 acaaagatac aacaaccaat gagatttaaa atcttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga taaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtctacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatgaccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaagg gcgatgaatc tttatatatt ctgaatattc acactcttta
    16321 caggaacttt ttagagcaat aggccatcag gagtatagtg atgcccgaca gaagaagtgt
    16381 tctgaatgaa agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg
    16441 gcatcccgct gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggagtc
    16501 tcgcagaaat ggtttgagtt cctcaacgct ggcaaacgca ttatcgcgcc catggccgaa
    16561 aggagagatg attattgcga aagccctggg aactgacccc tgggttatct ggccatcacg
    16621 ctaccatgat ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac
    16681 taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga
    16741 cgattactcg cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat
    16801 gcgttcgtag ccacgatcga tgtgataaat acgatcaacc accgtcgtcc cttccgcaat
    16861 acagccagcc agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc
    16921 agaaagtttt tcaacaccgt gacaaataac ggtattgctt tcgatttcgg catgcgcgcc
    16981 catacggcta agctccggca catgcataaa gcggttttca aagaccgttt cggtgatgaa
    17041 tccggtccct tctgccacca ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa
    17101 tgccggatgc ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag
    17161 gctaatccag tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt tcgccagcac
    17221 ggcgtcgaga gtatctggct gcgcgttacg gcagataatt ttgccgcgag aaatcgccgc
    17281 cgccaccagg aaagtaccgg tttcgatacg atccggtagc acgcgataga caccgccgcc
    17341 taaacgttcc acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc
    17401 cagcgtaatc aggaagttcg cggtatcgac gatttccggt tcacgcgctg cattttcaat
    17461 aatcgtggta ccttccgcca gggttgcagc acacatgatg gtcaccgttg cgccaacgct
    17521 gactttatcc atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta
    17581 accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt gtagatcaac
    17641 cggacgcgca ccgatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc
    17701 taccagcggc cccagtgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc
    17761 gcagaataca ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc
    17821 acccagctgg cttagcagct tcattgatgt atcgacgtct ttcagttttg ggacgttctg
    17881 gatctctacc ggttcttccg ccagtagagc ggcaaaaagg ataggcagag cagcattttt
    17941 agcgccggaa attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc
    18001 catttagttt gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca
    18061 aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac
    18121 agcatgaatg cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt
    18181 tttaacccga ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc
    18241 atcgccggaa acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc
    18301 attattttcc atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa
    18361 agttgcgctg gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg
    18421 caggcaggtt tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg
    18481 cagaacatca gcaggcaaat tatacaattt cgcgagggta tacactttgt cgtttacccc
    18541 ctgaagcgtc acgttgttgc cctgcttttt cgccagatcg ataagatgga gcagcagtgc
    18601 cagtccaccc gtatccacgc gggacacacg gctaagatcg atgcaggtaa tccccttcac
    18661 cgcttcctca cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa
    18721 cgccagcgtg tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc
    18781 agagtgattt tctgttgaga aatcgatttc agttgcgcag tcaggccgtc gatacctttg
    18841 gtacgcagca gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca
    18901 atcatgtcgt aagcctgcca gtttcccgtc tgggagtttt tacgccactg gaagtccaga
    18961 cgcaccggcg gacggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta
    19021 tcgcccagcg gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc
    19081 tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa agtaggcttc acgttgagca
    19141 ggggtcgcac tcttgtaata ctggcccagc accagcgcac cggcgtattt cacctgtacg
    19201 tatggcagca gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgc
    19261 ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttataa
    19321 ggattggtct ggtctgccgc ggttgccgca ctcagaggtg caatcaccag caaagcgacc
    19381 atcattaaac gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt
    19441 tcagtggttt cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca
    19501 tcgcctttac taccgtaaag gaactgacca atgagatctt ccagcaccat cgcagactta
    19561 gtgtcctgaa ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa
    19621 ccgacgttta atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa
    19681 ctggtatctg gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag
    19741 gttttcgggt ccagcgtaat atccgccacc cgacccacaa caacgccacc aatactgacc
    19801 ggagagcgcg ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt
    19861 tcagtacgta tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagcagt
    19921 gctgctaata aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt
    19981 cccaaacatc aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac
    20041 aacggtgcga gtggttgccc ggctaatccc ggcagacgtc gggatggtgt cgtagccgtt
    20101 aaacaacgaa atccacgtca ccgtgatggc gaacaccacg ctcttaatca gacagttgac
    20161 cagatccata cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat
    20221 gcctttccaa ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac
    20281 cgtcaacagt ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa cccgacgcag
    20341 cggatccacc gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat
    20401 ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc
    20461 cagttcacgc agtagcgata acgccaccag catacccaga ctggtttccg cactataagt
    20521 ggtcagaacc agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac
    20581 aataatcagc atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg
    20641 tttgcgaaat tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa
    20701 cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag
    20761 tggcttaact ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa
    20821 caggcccgtc agctatcccg tccagaaact gacgtacgcg cggatcagga ttcgcctgca
    20881 acgcctgggc actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatccg
    20941 caatacttaa cacttccggc acatcgtgag aaaccaccac acaagtcacg cccagcgcgc
    21001 tgttcagctc agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa
    21061 aaggttcatc aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac
    21121 gccgcgccat cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca
    21181 cggcctccag cttcatcatc accgtactat gcaacaatgg cgcgggtagt tgggtatgtt
    21241 cgcgcagtgg ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gccccggact
    21301 gaaataacat gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa
    21361 tattctcacc atcgaaaagg atctcaccat gatctggtgc gatttgcccg ccaatcagac
    21421 ggagtagcgt cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg
    21481 gcacggtcag ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc
    21541 gcatatcgac taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt
    21601 ttctaagcat ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc
    21661 gtggttagcg aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa
    21721 tccgttacgg ctttctgaaa atcttcagcg gaccgacgag tatacctgaa gaaaggacgt
    21781 tagatgcttt tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc
    21841 gaccgcctgg tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctgatc
    21901 atcggcatga cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc
    21961 gcgtctctgc acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc
    22021 aatattttgc tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattccgat
    22081 gttctgcgtc gtgaattacc cttaatgttg ttggtcagcg tagtggccgg ttccgtactc
    22141 tatgacggac aacttagtcg cagcgatggt atctttctcc tctttctggc tgtgctatgg
    22201 ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc
    22261 agagagcagc ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc
    22321 ggcattgcgc ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg
    22381 ctggcgaatt actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga
    22441 accagcctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt
    22501 gctgtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc
    22561 gcgctgataa cgccaggaga gattgagcca ttggcgtaca gtcgtgacta cagcgtgatg
    22621 ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt
    22681 ggtgtagggg tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta
    22741 tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg
    22801 ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga
    22861 gcttgatcaa tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa
    22921 agggaaagtt gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc
    22981 aacgtttgcc agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg
    23041 tgatttaggc atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc
    23101 cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat
    23161 caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt
    23221 agcgaaagaa gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt
    23281 tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt
    23341 tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat
    23401 tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcgtt
    23461 gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat
    23521 tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg
    23581 tcagttaagt attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct
    23641 ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga
    23701 tggcgaccat ttactcggag tgttacatat gcatgattta ctgcgtgcag gcgtagtgta
    23761 aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc
    23821 cctgtcagcg ccgacgttat agcaaaagca gagaacattc gtctgctgat cctcgatgtc
    23881 gatggcgtac tgtcagatgg cctgatttat atgggcaata atggcgaaga gctgaaagcg
    23941 ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct
    24001 atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact
    24061 cacttgtatc aggggcagtc aaacaaactg atcgccttta gcgatctgct ggaaaaactg
    24121 gcgattgccc cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg
    24181 gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc
    24241 gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta
    24301 ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac
    24361 gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt gatatggccg
    24421 aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc
    24481 atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac
    24541 acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt
    24601 ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg
    24661 accggatgct ctatttatat ggacacgttg aagtcaacgc acttgtgcca aactctcaac
    24721 ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg
    24781 aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca
    24841 acttacgcag caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa
    24901 acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg
    24961 gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct
    25021 cttgatatgc aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc
    25081 atcaaaatta atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa
    25141 gtgattgacg gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc
    25201 gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg
    25261 ggtaatgctt atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg
    25321 gtaaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg
    25381 gtgccgtcgc agctgcagga caaaaacaac aaaggccaga ccccggcaca gaagaagggt
    25441 aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt
    25501 cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg
    25561 ccaaacggtg ccggtaagac caccactttc tacatggttg taggcattgt gccgcgcgat
    25621 gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg
    25681 cgccgcggta tcggctatct gccacaggaa gcctccattt tccgtcgcct cagcgtttac
    25741 gataacctga tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac
    25801 cgcgcgaacg agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag
    25861 tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg
    25921 aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt
    25981 aaacgcatca ttgagcacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac
    26041 gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc
    26101 gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg
    26161 gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc
    26221 tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc
    26281 tccaacaggc aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc
    26341 aggcgctgga gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc
    26401 gcgaaacgca agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc
    26461 cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg
    26521 gcaccagcgg tgactacatt gacgatgagc tgccggtcta tcagggcgaa acgacgcaga
    26581 ccttgcagga ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg
    26641 ctattgctac ctctatcgtc gatgccgttg atgacatcgg ttatctgact gtcccgctgg
    26701 aagatattct cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc
    26761 ttaagcggat ccaacggttt gatccggtcg gtgtagcggc aaaagatctg cgtgactgcc
    26821 tgctaatcca actctcccaa ttcgataaaa ccacgccgtg gctggaagag gccagactga
    26881 tcattagcga tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca
    26941 cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc
    27001 gccccgggca gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc
    27061 gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa
    27121 tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta
    27181 tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata
    27241 cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg
    27301 aagaatatat gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat
    27361 cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac
    27421 tgaagtattt cttttccagt cacatcaata ccgagggcgg cggcgaagct tcctccacgg
    27481 cgattcgtgc gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg
    27541 acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg
    27601 cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac
    27661 ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc
    27721 gaggcactgc gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga
    27781 atcaaccagg tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca
    27841 ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc
    27901 attgatggct taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa
    27961 caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg
    28021 ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt
    28081 gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt
    28141 acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg
    28201 gcggcgaaac aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa
    28261 aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa
    28321 gatactctgc gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc
    28381 atcgacaacc aaccggtgga cttacttttt gccctgctgg tgtcggcaga ccaaactaaa
    28441 acgcacctgc atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc
    28501 cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt
    28561 actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag
    28621 tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt
    28681 gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat
    28741 ctggctcgaa ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat
    28801 atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca
    28861 ccgcaactcc tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg
    28921 cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa
    28981 agcgatttgc tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg
    29041 tccgttcacg agctggctga aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa
    29101 ctgaccatgg tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac
    29161 gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca
    29221 ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc
    29281 taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc
    29341 tacttgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag
    29401 caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg
    29461 gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc
    29521 atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt
    29581 tacgtaatga cgaaggcacc gaggccgaag ccaacagcgt tattgcgctg ctgatgttgg
    29641 attctgccaa aggacggcag attgaagttg aagcgaccgg cccacaggaa gaggaagcac
    29701 tggccgccgt tatcgccctc tttaactctg gttttgatga agattaatct tcatcacttt
    29761 gacatacaac tcccttcaaa ctccccccga caataagaaa atcacgtact gaaatcgttc
    29821 tcaatcaacg tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa
    29881 ccttaagagg cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga
    29941 aaatatttcc aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa
    30001 gacatcggaa atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc
    30061 atcgtggcga tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg
    30121 gtcgccggtt aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt
    30181 gcggaaccgg ttatgtctac gatgtgattg cagattttga cggcaaactc tccattacgc
    30241 tgaccgaatt tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac
    30301 tgaaacagct gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc
    30361 agaatatcct ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca
    30421 ttggtgaaag cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc
    30481 aagagcggtt atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag
    30541 atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct
    30601 ggtgttgttg cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct
    30661 gacggctacg cacgtagccc gatggcgagg agactttgaa acgaagtgga ttaggtaata
    30721 cagctgccag taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac
    30781 gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa
    30841 ccgtcaggat acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca
    30901 gcccttttcg tacccagcta cgcccatccc ataaaaagag atttttggcc gtctgctgag
    30961 aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggctttct
    31021 caatggaagc gacatcaaag ccccagtgct cagaaaattt ctgatcttct gcggcaatca
    31081 ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt
    31141 aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc gctgagaagg
    31201 gaacaggcgc aacactaaac aacgcgatgc ccccgcccca gaaaacggcg aggacaacca
    31261 ttaacagcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc
    31321 agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt
    31381 ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa ccacgatatc
    31441 atcgacagga cagggcacat gctccgcgcc catctcttcc agcacttctg cggtatcgat
    31501 atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc
    31561 aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa
    31621 ttcacggtca acggtgcatt cgctaccaag agtggcaaaa ttgcttaaat tcttcgccgc
    31681 gccaaacccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg
    31741 acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acgttgcgcg tttccgtcat
    31801 cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac
    31861 cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat gaatttcaga
    31921 accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcatcct
    31981 ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat cacacattta
    32041 actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa
    32101 cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt
    32161 gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca
    32221 cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt
    32281 caccgacgtt atcttcccag gcaggaaggt caggagactg aatttgctga cccagctgtt
    32341 gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct tcctcaacaa
    32401 tgcctttttt atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca
    32461 tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact
    32521 gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg
    32581 tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa
    32641 cagaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct tgtttgtctt
    32701 tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctctcgcgga taacgcttcg
    32761 tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc agtaacacca
    32821 ggtcgtattc gcccggttta aacatctcta gcgccgcctt gccggtcatg gcgacatcaa
    32881 cgctgttacc taatttttcc agcacagaac gcgcaacaat cacgttcagt tcaatgtctt
    32941 ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga
    33001 cctcttctgc taccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt
    33061 cgctggtaac cgcaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac
    33121 caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg
    33181 cgaaaatttt atccagctca tcctgcggaa tgccgatacc agagtcttcc acttcaaaat
    33241 gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt
    33301 tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg tcggtaatga
    33361 cctgatgcgg taatggcagc gtcggctcca ggttaaagcg caatcctttt tgttgcgcct
    33421 gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggttgattat
    33481 caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta aagatattcc
    33541 ccagcgtgac ggccgaaaca tggatggtct tgagatattt ttcctgctcg gcggtgagtt
    33601 cggtatccag cagaatgcgg ctcagaccga cgataccgtt cagcggtgta cgcaattcgt
    33661 gactgatggt ggagataaac gtcgttttgt cgcggctggc ccgttcaagc gcatcctgat
    33721 accgcttacg ctcggtaatg tctcgaccaa agcccatcaa accgtgacgt ttacccacgc
    33781 ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca
    33841 gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga
    33901 cttttgcggc ggcttccggt gagtaaacat caacaggttt caggtgaacc agttgttttt
    33961 cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt
    34021 tacgataaaa aaccaggtcg ggtgaagcat caaggaagga acgtaagaat gaggattgct
    34081 gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg
    34141 tttcctgcag ttctgcttcc gctttttcac gaacggcgat ttcctgattt agctgggcaa
    34201 tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt
    34261 gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa
    34321 agtagaccgc ccacggcgta atcaacaaac caaagaagat agaacgaata acatcaatgc
    34381 tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga
    34441 cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa
    34501 cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc
    34561 cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc
    34621 ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc
    34681 atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt cgcaccacag
    34741 aggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg
    34801 tggcgtatgg cgaatcattt ctccggcagt gagcgtgtaa tcctccagtt caataccgtt
    34861 caaacgcccc gcttcccagg ctttcgccat aatgctgcct ttcacaatat gcaacggatg
    34921 tagctttata ccgtcaacac cggtttccac tacccgctcc agcgtttgca gacattcagc
    34981 cagcccttcg cctggcagac cgacaattaa atgcgagcac accttcagcc cgcgctggcg
    35041 tgccagctgc gtcgtgcgct gataacaggc gaaatcatga ccgcgattaa tgcgatgtag
    35101 cgttttgtcg tgcacggttt gcagccccag ctccagccac acttcgtagc cctggtcttt
    35161 atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcgcg tgccgacaca
    35221 caaaccgaca atattggcct ggctcaccgc ctgttgatac atcgaacgca gcacctgaac
    35281 ttcggcaaag gtgctggtat acgcctggaa ataggcgagg taacgtttcg cgcgattaat
    35341 taaattcgcc tgatgcgcca gctgttcggc aatggaacga tgctgctgcg cttcgtcggc
    35401 aaacgaggca acattacaga aggtgcagcc gccgcgcccg atggtgccgt cgcgattcgg
    35461 gcaactgaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag
    35521 atcaccacca aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg
    35581 aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctgga tcaatcgtcc
    35641 cggcccgctt ttatcaactg cataatcaat caaaattacc gaaatttcat gcataatcac
    35701 ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa
    35761 aaacagtagc atgaaacgtc attaccaatt aatgcagtat aaaatgctgg ttttctcgtc
    35821 aattcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg actgtttctc
    35881 taacgacttc ccttttagcc ttaaagataa aatctatttt aatttcagcc atttaataaa
    35941 gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct
    36001 ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt
    36061 tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg
    36121 ggcgaatgcg aggcgcgcgt atgacacgca acccccgtcg ccacgctctt tctgtacccg
    36181 tgcgcagcgg ttcggtagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt
    36241 acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg
    36301 aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg
    36361 gcgcgattct cgccgatggt aaaaccggtg acggttgcgg cttgctgtta caaaaaccgg
    36421 atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcgaaa aactacgccg
    36481 tcgggatgct attcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg
    36541 aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg
    36601 aaggcgtact gggtgaaatc gccctctcct ctctgccccg cattgagcaa atttttgtga
    36661 acgccccggc aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc
    36721 gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagccta tcgaatctgg
    36781 tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg
    36841 cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg
    36901 taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt gaaatcaaca
    36961 ccatcactgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctta
    37021 tccctgatct gcacgacgcc gcaccgttcg tcaacgaaac cggctctgac tccagttcga
    37081 tggataacat gctggaactc ctgctggcag gcgggatgga tatcatccgc gccatgcgtc
    37141 tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct
    37201 ttgactttaa ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtccg
    37261 acggtcgttt tgccgcctgt aacctcgacc gtaacggtct gcgtccggcg cgctacgtca
    37321 tcaccaaaga taagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg
    37381 acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggttatc gacacccgca
    37441 gtgggcatat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc catccatata
    37501 aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag
    37561 aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact
    37621 acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg
    37681 tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt
    37741 acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg
    37801 aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag
    37861 cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca
    37921 aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg
    37981 acgtcactaa aaccacgctc gaagcgacag tcaaagagct gtgcgacaaa gccgaaaaaa
    38041 tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc
    38101 tgccggttcc agccccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc
    38161 tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg
    38221 ccgtgttgct gggcttcggc gcgacggcta tttatccata ccttgcctat gaaacgctgg
    38281 gccgcctggt agacacccat gcgattgcca aagattatcg taccgtgatg ctcaactacc
    38341 gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg
    38401 cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat gtagtgggcc
    38461 tgtgcttcca gggggcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg
    38521 atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgtaa gcccatcagc cagggcggcc
    38581 tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc
    38641 tgcaacaagc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta
    38701 atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg
    38761 tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgcttcgat accgctgcga
    38821 tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaacagca
    38881 tcggcggtaa ctcaaactcc ggtgaaggcg gtgaagatcc ggcgcgctac ggcaccaaca
    38941 aagtgtcgcg catcaagcag gtggcttccg gtcgctttgg ggttactccg gcgtatctgg
    39001 tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc
    39061 agttgccggg tgataaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag
    39121 tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaggac ttagcgcagc
    39181 tcattttcga cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg
    39241 aaccgggcgt aggcaccatc gcgactggcg tggcaaaagc ttatgccgat ttaatcacca
    39301 tcgcaggcta tgacggtggc accggcgcaa gcccgctgtc gtcagtgaaa tacgcaggtt
    39361 gcccgtggga gctggggctt gttgaaaccc agcaggcgct ggttgccaat ggtctgcgtc
    39421 ataagatccg tttgcaggtc gatggcggcc tgaaaaccgg tgttgatatc atcaaagcgg
    39481 cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtcgcg ctcggctgta
    39541 aatatctgcg tatttgccac ctgaacaact gcgcaacggg tgtggcaact caggatgaca
    39601 aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gacgaattac tttgagttta
    39661 tcgcccgtga aacccgcgag ctgatggcac agctgggggt aacgcggctg gtggatctga
    39721 ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg
    39781 cgctgtcgaa gctgctggag accgccgaac cgcatccagg taaggcactc tactgcaccg
    39841 aaaacaaccc gccgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac
    39901 cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaat accgatcgct
    39961 ccgtgggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag
    40021 ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtatggaacg
    40081 cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg
    40141 cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa
    40201 gtattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc
    40261 gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg
    40321 gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg
    40381 ttaacttcgg tgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt
    40441 tccgcaaacg cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc
    40501 atgaagagca tctgcgcggt cttatcaccg agcatgttca gcatactggc tctcagcgcg
    40561 gtgaagagat tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga
    40621 agtccagtga tgtaaaagca ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg
    40681 tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc
    40741 agcgcgttga tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt
    40801 acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca
    40861 acccatactg cgagtggaaa tgtccggtac acaactacat cccgaactgg ctgaagctcg
    40921 ccaacgaggg gcgtattttt gaagcggcgg aactgtcgca ccagaccaac accctgccgg
    40981 aagtttgcgg acgagtctgc ccgcaagacc gtctgtgcga aggttcctgc actctgaacg
    41041 atgagtttgg cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg
    41101 aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta
    41161 tcggcgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag
    41221 ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct
    41281 tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg
    41341 aattcaaact caataccgaa gtgggccgcg acgtgcagct ggacgatctg ctgagtgatt
    41401 acgatgccgt cttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg
    41461 aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa
    41521 tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtgg
    41581 tccttggcgg tggcgacact gcgatggact gcgtgcgtac gtccgtgcgc cagggagcga
    41641 agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag
    41701 tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg
    41761 aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac
    41821 cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg
    41881 cagatgcagt gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac
    41941 acagcgtcga gctggattca caaggccgca tcatcgcccc ggaaggcagc gacaacgcct
    42001 tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg
    42061 tggtaaccgc tattgccgaa ggtcgtaagg cggcagacgg tattatgaac tggctggaag
    42121 tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc
    42181 tttctggaaa gcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg
    42241 ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg
    42301 gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg
    42361 gtattattca cctggtcgat agcgcccgga cggaaacggt acgtagcgtt aacgcgctga
    42421 tgaccgcgac atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg
    42481 caagggctgc gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt
    42541 ataagcgtgg tttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc
    42601 aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga
    42661 caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc aaattgagag
    42721 gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt gtatattcat
    42781 gcaaataaac gaaggaaagt tgccgtgatt tacattgaac agcttgaatt aatccataaa
    42841 agtggtgatg tgttgtatcc agtaaaaata accagaaaat catcaggaaa aactgccttc
    42901 caccttgttc cattcggctt aaataagact cacgaccttc tagaggtgga agatgcttca
    42961 gaagcaattc ggctggtgat tgatgaacgt cattccattc gctgcagtac acttacagca
    43021 actattacga ataaaaaggg caagcgaatt aagcggacag gtatatacag tatcaagggt
    43081 gttaacataa aagaatacaa cgtcaggtaa tggcacagag cggtccgtca gttcaggttt
    43141 ggctctgtgc caaagatggg tcaggtcaca cctgatctaa taaatttaag ccattaaatc
    43201 agccttaacc ttcaccacca cctttttaat ctcacccggc tcgccaacaa cgcatcccgg
    43261 cttatgcggc tctcctagca taaacacggc gaacattccc ggtttcagga tgatggcttg
    43321 ctcgttttca atggtgctgc aaagctggta atcatcctca tggtggaact cttcacactg
    43381 acgcgcagtg cctgccatgc caaacagaat ccgttcttca ccgtttaata acagctggat
    43441 atcaatgtat tgctcgtgca gctccgcttt tttctcaacg ggcgattgag tgttaaacgt
    43501 catgacattc ataaaaatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg
    43561 cggtctggca gctaatgcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga
    43621 cggtaatgac cgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgctgcc
    43681 ccaagtaaac ctgcatcatg gcggtagtgc gccgccagta aatcaacatg aaataccgct
    43741 ggctcctgcg ccagatacgt ttccaccaac gccagatacc cttctgccag accaacgctg
    43801 ccaccgacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgcc
    43861 agcacatgtg cggagcggtg aatcagttgc tgcgcctgct cgtcgccctg cccggcgcgt
    43921 gtgaaaatag ttctcgcatc cgcgcccatg agttcacctt gcgctgccgc tgcaataccg
    43981 cgaccagaag caatcgcttc cacgcaacct gtgcgtccac agccgcagac tgggccgtgt
    44041 ggatcggcaa gcgtatgccc gatatgcccc gccagaccgc cagggccggt aagcagttta
    44101 ccgccgctca ctacaccgcc gccgacgcca gtggaaacgg tgataaacac catctcggtt
    44161 acatcgcctt ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca
    44221 atggtcggca aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca
    44281 ccgagattat gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg
    44341 atggcaaccc gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc
    44401 aaggcttctg gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg
    44461 tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagtgtggtc
    44521 atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa
    44581 tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag
    44641 gcgtgttgta acgcccttcg gcaatcaccc gacatccggc ttcgctcaac gttttcacca
    44701 gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa
    44761 taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca
    44821 tcgccagtaa accgtgatga tgtatacgtg ccagcagcgt ttcaacaggc accggacgcg
    44881 ggcggtcggt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat
    44941 cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa
    45001 taatcggcac gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa
    45061 tggcaaccgc gcccgcctgc tctgccgcta atgccatggc tgcgacgatt tcgggtttat
    45121 cgagcgggct gtcaggaatc ggctggcagg agacaatcag gccaccgtta gcagcgattt
    45181 tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata
    45241 gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc tgccaaatgg
    45301 cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg
    45361 cagccaacgc tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt
    45421 caggctgaac gagagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc
    45481 gccgatgatt ggggccagtg cgccacccaa tgcgccaacg ttgtaggtaa agcccaggcc
    45541 cgctgcacgc tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat
    45601 cccttgtcca agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc
    45661 gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcaggctac aaacgtacgc
    45721 tttgcgggtt cccagccagt caccgaggaa gcctcctacg cagcatccca cggccgcgcc
    45781 aaagccacta aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt
    45841 cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac
    45901 caccatcagc attacgcccg ttggccagcg tttgcctgta ctctgcacca taaagctgat
    45961 aaagattgcg gcgcataaca gcccaagaac agcgacgatc gcggcatttt gcaggttacc
    46021 ggcgaagcag aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg
    46081 atgttcacca cggtagagaa tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt
    46141 ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgattgg
    46201 caaaatgccg ataaagaaca gcgcacgcca accccagacc ggaaccacca ggctatagac
    46261 ctgagcggca acgacggccc ccacagagaa gcctgaaatc aaaaaaccac tggctttgtt
    46321 acgcagatgt tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc
    46381 catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc
    46441 gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc
    46501 gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc
    46561 ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta cttcggtgag
    46621 taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga
    46681 aaatgcgcgc cattgtgcac ggttgagatg gcgataccac gggatattct gggttgtagt
    46741 actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg
    46801 gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc
    46861 cgcgctcttg catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta
    46921 ccggtccaaa cggtttgcgg cacagcggca cagaaacgac atccatataa tgcaggacag
    46981 ttttcaggcc gcggaatacg ccagttttga tcagtaaatc aatgactttg ttgcactcag
    47041 tttgcagttt ctgcgcggtc tggatatcgc cttctttcag cgccttcacg atcccctgat
    47101 atcgccagcc catgatgttg taagtactgc cgataccacc atcagcgcct gccagcaagc
    47161 cggaagcgaa gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga
    47221 tctgttccat ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg
    47281 taaccagggt gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt
    47341 acacaaccat cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt
    47401 cttcaaagct gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac
    47461 gtttagccga tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga
    47521 tgagtttaat cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg
    47581 aaaggctttg tacaaaggcc tcgccggtcg aaccacccac gtataaaccg tcgatgccct
    47641 gctgaatatt gaactgaacc aggcgacgca gactcgcttt atccagtgct tgttgttggt
    47701 caaaaggagt caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg
    47761 aagtgatact tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc
    47821 aacaccccat acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt
    47881 ttgttggtgg tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc
    47941 aacgcacgat cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc
    48001 tgttgatagc taacgttgtt atgctcgtgc agagcctgat cagccacggt tggacgtgcg
    48061 gcgatcagcc agtcgagcag ggcaacgtgg atcgccatga agattgggtt accagggatc
    48121 tccgccagca cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc
    48181 gactgactgt tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcatgt
    48241 tcagccgcat agcgcaccag actggattca aagaacagac gtaattgttc gaaatgggca
    48301 atcccaccgg gatgagaaag gaaatctttc gccatgccgg aaaactcacc gatgatagtg
    48361 tccgcagaag gacgcgagac gcgagcgcgt tcgccgttgt ttatttgcac cagacctttg
    48421 cgttttaacg ctgccagcgc ttcgcgcaca gaaggacgcc cgacgttaaa gaacgccatc
    48481 agttcgcgtt cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt
    48541 tccagctctt cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag
    48601 ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc
    48661 attctgtaag gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta
    48721 ggggaaaatg gcaggttttt ctctttgtgc ctcatcatta ccataattaa cggaataatt
    48781 aactattgcg aaaaattaat gtaacgcaga taaacacatc ccgattgaat tattcataag
    48841 actattcaca agcattatga atattatgaa tgtgttctta caaaataatc ataagcgcat
    48901 attttttaat gaaaaatcac atcacctaca attaaaaaca cgacatccgc accataaata
    48961 gacttgcaaa aaatataaca tcattgtttt caatctgccg tttatgggat tgaccgtttt
    49021 cttttgacac ggagttcaac aatgttcggc ataattatat ctgtcatcgt attaattacg
    49081 atgggctatt tgatcctgaa aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc
    49141 ttcctgatga tgtgcggagt ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc
    49201 agcggctact tgatcgtcga catttataat gaaatcctgc gcatgctgtc caaccgcatt
    49261 gccggattgg ggctgtcgat tatggcggtg ggcggttatg cccgctacat ggagcgcata
    49321 ggagccagtc gcgcgatggt gagcttgcta agccgcccgt taaaactcat tcgctcgccg
    49381 tatattattc tgtcggcaac ttacgtcatc ggccaaatca tggcgcagtt tattaccagc
    49441 gcctccggtc tgggtatgtt gctgatggtc accttatttc cgacgctggt gagtctggga
    49501 gtaagtcgcc tctctgcggt ggcggttatc gcaaccacga tgtccattga gtgggggatt
    49561 ctggaaacga actccatttt tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc
    49621 ttccactacc agcttccggt cgcctcttgc gtcattatct cggtggcgat ctcccacttt
    49681 ttcgtgcaac gcgcttttga caaaaaagat aaaaatatca atcacgaaca ggcagagcaa
    49741 aaaactctcg ataatgtccc gccgctctat tacgccattt tacctgtgat gccgttaatc
    49801 ctgatgctcg gctcgctgtt cctcgcccac gtcgggctga tgaagtcaga actgcatctg
    49861 gtggtggtga tgttactaag tttgacggtg acgatgtttg ttgagttctt ccgcaagcat
    49921 aacttgcgcg aaacaatgga cgatgtgcag gcgttttttg acggcatggg tacgcagttt
    49981 gccaacgtgg taacgctggt ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt
    50041 ggcactgttg atgcggttat caggggtgcg gagcattctg gtctgggcgg tattggcgtg
    50101 atgattatta tggcgctagt cattgccatt tgtgccattg tgatgggctc tggcaatgcg
    50161 ccgtttatgt catttgccag tcttattccg aatatcgcag ccggactaca tgtaccagcg
    50221 gttgtaatga ttatgccgat gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt
    50281 actgcggtgg tgaagcggac agcgatcccc atggcagtcg gtttcgtggt gaatatgatt
    50341 gccacaatca cgctatttta ttaagtcatt aaaaagacaa aacaggccgc ctgggcctgt
    50401 tttgtattac ttcacaacac gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc
    50461 atcgtcagga tgagtgtcat catcgtgatc tggcttgtcg ccatcaataa ccgacataac
    50521 ggtttcgttg tctgccgatg cctcttcatc attcatgatg ctggtatctt catcgtaggc
    50581 agcttcaggc tcaaacatcg tgcctgcgcc attttcacgg gcgtagatag ccagcacggc
    50641 agccagcggc acagaaacct gacgcggaat gccaccaaag cgcgcgttaa agcgcacctc
    50701 atcattcgcc agttccagat tgccgacagc acgcggcgca atgttgagta cgatttgccc
    50761 gtcacgcgca tattccatag gaacctgcac gccagggagc gtcacatcca ccaccaggtg
    50821 cggcgtgagc tggttatcca gcaaccactc atagaatgca cgcagcagat agggacgacg
    50881 tggtgttagc tgtgacaaat ccatacagat taactccggc ccagacgcat ttcacgttct
    50941 gcttcagtta aagaagcaag gaaagagtca cgctcaaaga cgcgggtcat atagcctttc
    51001 agctctttcg cacccgggcc gctgagctcg atacccagtt gcggcagacg ccacagcagc
    51061 ggagcaagat agcaatcgac caggctgaac tcatcgctca ggaagtacgg cttctgaccg
    51121 aagaccggcg caatcgccag cagttcttcg cgcagttgct tacgtgcggc atctgcttca
    51181 gaagctgaac cgttgatgat ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc
    51241 atgtacagac ggctttcacc gcgagctacc gggtaaacag gcatcagtgg cggatgcggg
    51301 aaacgctcat ccagatattc cataatgatg cgagattccc acagggtcag ctcacgatcc
    51361 accagggtcg gaacgctctg attcgggttg aggtcaatca gatcctgagg cggattgtcc
    51421 ttttccacgt gttcgatctc gaaacttaca cctttctcag ccagcacaat gcggacctga
    51481 tggctataga tgtcagtagg accggaaaac agcgtcatta ccgaacgttt gttggcagcg
    51541 acagccatga aaacctccag gtatagtcag aatttttact gctaccagcc accaggtggc
    51601 cagtcagaag ttgtgttacc caataaggaa cgactctctt tgttcgaaaa tcaaacaaaa
    51661 aatgagcaat acccgacatt tgggcagaaa attggatgat agtttaccag attttgcgac
    51721 cattgtggtg agtggatgcc ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt
    51781 tgacttagac ccttattggc gatgtggttt ttgttttgcc tgtctttcag acggcagcaa
    51841 aaagcaactt cccatttttt acgctgattc agaatttagc cataaaaaaa cccgccgaag
    51901 cgggtttttt cgtaaattgt tttctgccgg agcagaagcc aattaacgtt tggagaactg
    51961 cggacgacga cgtgctttac gcagaccgac tttcttacgt tcaacctgac gagcgtcacg
    52021 agtaacgaag ccagctttac gcagttcaga acgcagggac tcgtcgtatt ccatcagagc
    52081 gcgggtgata ccgtgacgga tcgcaccagc ctgaccagag ataccaccac ctttaacggt
    52141 gatgtacagg tccagtttct caaccatgtc gaccagttcc agcggctgac gaactaccat
    52201 gcgggcagtt tcacgaccga agtactgttc cagagaacgt tggttgatta cgattttacc
    52261 gttgcccggt ttgatgaaaa cgcgagctgc ggaatttttg cggcgaccag tgccgtagta
    52321 ttgattttca gccattgcct ataatcccga ttagatgtca agaacttgcg gttgctgtgc
    52381 cgcgtggttg tgctcgttac ccgcgtaaac tttcagttta cggaacatag cacgacccag
    52441 cgggcctttt ggcaacatgc ctttaaccgc gatttcaatc acacgctcag gacggcgagc
    52501 aatcatctct tcaaagctca cttgtttgat accaccgatg tggccggtgt ggtgatagta
    52561 cactttgtca gtacgcttgt tgccggttac agcaactttg tcagcgttca gaacgatgat
    52621 gtaatcaccg gtatctacgt gcggagtgta ttccgctttg tgcttaccgc gtaggcgacg
    52681 agccagttca gtagccagac ggcccagagt tttaccggtc gcgtcaacaa cataccagtc
    52741 gcgttttacg gtttctggtt tagctgtaaa agttttcatt aaaagcttac ccaataaata
    52801 gttacacgtt ggtgaacacc caaacgtctt caattgttga ggttcacacg acaaagtccg
    52861 gcaaacctac cccttcgaat agcctatgcc agcacacaaa aagttttggg aaaaaaactt
    52921 tcttgtaacg tggggtcgca ggattataga gaagtcgggg tcaaagatcg accccttttt
    52981 gtgatttgtg acaggtttta acccgccaaa tgctcgcgct tcagatactc ttcgctttgc
    53041 atctcttgca gacgtgacag gcaacgctgg aactcaaact tcagccgatc gccctgataa
    53101 atttcataca gcggcacttc tgcactcacc actaatttga catggcgctc gtaaaactca
    53161 tccaccagcg caataaagcg ccgcgcttcg ctctccatca accgcgtcat aactggtaca
    53221 tcaaacaaca tgaccgtatg aaagagacgt gagagcgcaa tatagtcatg ctgactgcgg
    53281 gcgtcgacgc acagcgtagt aaaagagacc gccagcgtct ggttctcgac gcccattgtc
    53341 gctaatggcc gatggttgat ttctaacgtc ggtgaatttt ctcgtttccc ccccgccagc
    53401 gccaaccata gtttatccat ttgcgcccgg gtttcatcgt ggagtggcga aagccacaga
    53461 tgcgcctgag tgagtgtacg cagtcgataa tcaacaccag cgtccacgtt cattacatca
    53521 caatgctgtt taatggcatc gattgcaggc agaaaacgcg cacgttgcaa gccatttcga
    53581 taaagttcgt ccggcggaat atttgacgtc gctaccaggg taataccgcg ggcgaacaag
    53641 gctttcatca gaccgccaag tagcatggca tcggtaatat cagaaacaaa aaattcgtca
    53701 aaacagagca cgtcagtttc ggctttaaag cgatcggcaa taatttccag cggatcggtc
    53761 tgcccctgta aggcagttag ctcttcatgc acccgcagca taaaacggtg aaagtgcagg
    53821 cgctgtttcc gctctcccgg caggctttga tagaaaaggt ccatcagcca ggttttcccg
    53881 cgtcctacac cgccccacat atataagcca cgcactggcg tatgctttgt gtcttcgcgt
    53941 ttaccccaca gcttaccgac ccgcgccatt agcccactcg tcctgggggc tggtggcgtg
    54001 ctattgatga gttcctgata aataatttcc aggcggctga cggcctcttt ttgaacgtcg
    54061 tcgggttgat ggctgccttc attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt
    54121 tgcatgatct tattgttatt ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa
    54181 aaaaggccgt tctacactac gcgatatgca gtcgggattc cacttctgtg gaattaacgg
    54241 ttatagtggc ataatcagcc gcaggcatgg agcctgaagc caacacccta cggaaacaaa
    54301 agacaacggg agatgttcat gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc
    54361 attattggtg ctgtggccat gcgttttggt aatcgtaaac tacgccagca acaggcgttg
    54421 cagtacgaac tggaaaagaa taaagctgaa ctggacgagt atcgcgaaga gctggttagc
    54481 cactttgccc gcagcgcgga attactggat accatggcgc acgactatcg ccagctgtat
    54541 cagcacatgg cgaaaagttc cagcagcctg ctgccggaac tgtctgctga agcaaacccg
    54601 ttccgtaatc gtctggcaga gtctgaagcc agcaacgatc aggcaccggt gcagatgcct
    54661 cgcgactatt ctgaaggcgc atccggcctg ctgcgtactg gcgcgaagcg cgactaattt
    54721 atttttcggg cgcagccatt gcgccctcct cttctctccc tccccgacta tcatttaatc
    54781 tggtgtctca ttgttagccg tctgaaaatt caataacatc aaactgtttt gaatctcttt
    54841 tcttatcatt caggtacgag agcaggaata atgaaaaaac aaccccagct gttgagtgca
    54901 ttagcgttaa gtgtcgggtt aactctctcg gcgtcatttc aggccgtcgc gtcgattcca
    54961 ggccaggttg ccgatcaggc ccctctcccc agtctggctc caatgctgga aaaagtgctt
    55021 ccggcagtgg tgagcgtacg ggtggaagga acggccagtc agggacagaa aatcccggaa
    55081 gaattcaaaa agttttttgg tgatgattta ccggagcaac ctgcacaacc cttcgaaggt
    55141 ttaggctccg gtgtcatcat caacgccagt aaaggctatg tgctgaccaa caaccatgtg
    55201 attaatcagg cacagaaaat cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa
    55261 ctgattggta gcgatgacca gagcgatatc gccctgttac aaattcaaaa cccgagcaaa
    55321 ttaacgcaaa tcgctattgc cgactccgat aaattgcgcg tcggtgattt tgccgtagcg
    55381 gtcggtaacc catttggcct tgggcaaacc gccacctctg gcattgtttc cgcattagga
    55441 cgcagcgggt tgaatcttga aggtctggaa aactttatcc agacagatgc ttccattaac
    55501 cgcggtaact ccggcggtgc actgttaaac cttaacggtg agttaattgg catcaacact
    55561 gcaatccttg cgcctggcgg cgggagcgtc gggattggat ttgccatccc cagtaatatg
    55621 gcgcgaacac tggcgcagca gcttatcgac tttggtgaaa tcaaacgcgg tttgttaggc
    55681 atcaaaggca ccgagatgag tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt
    55741 ggcgcgtttg tcagcgaagt gttgccaggt tctggctcgg cgaaagcggg cgtcaaagcg
    55801 ggcgatatta ttaccagcct caacggcaaa ccgctgaata gctttgctga gttgcgctct
    55861 cgtatcgcga ccaccgagcc gggcacaaaa gtgaaacttg gcctgctgcg taacggcaaa
    55921 ccactggaag tagaagtgac gctcgatacc agcacctctt cgtcggccag cgctgaaatg
    55981 atcgcgccag cgctggaagg tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa
    56041 ggtattaaga tcgatgaagt tgtcaaagga agcccagctg ctcaggctgg cttgcaaaaa
    56101 gacgatgtga tcattggcgt caaccgcgat cgggtgaact cgattgctga aatgcgtaaa
    56161 gtgctggcgg caaaatcggc catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc
    56221 tatctgctga tgcgttaatg tcgtaaaccg ggcatcaggc ttacgtgtga tgtccggtta
    56281 actcgtggta tgctgctgcc gttccctttt ttaatgacgc ctccatcatg tttgtgaagc
    56341 tcttacgttc cgttgcgatt ggattaattg tcggcgctat tctgctggtt gccatgcctt
    56401 cgctgcgcag ccttaacccg ctttccactc cgcaatttga cagtaccgat gagacgcctg
    56461 ccagctataa tctggcggtt cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg
    56521 gtttgaacac caactctcac aaccagcttg agatccgcac cctgggatcc ggtgtaatca
    56581 tggatcaacg cggttatatc atcaccaata aacacgtcat caacgacgcc gatcagatca
    56641 tcgtcgcctt acaggatggt cgtgtatttg aagcattgct ggtgggatct gactctctaa
    56701 ccgatctggc ggtacttaaa attaatgcca ctggcggttt acctaccatt ccaatcaatg
    56761 cacgtcgcgt accgcatatt ggcgacgtgg tactggcgat cggtaacccg tacaacctcg
    56821 ggcagaccat tacccagggg attattagtg ccacgggtcg aattggtctg aacccgaccg
    56881 ggcggcaaaa cttcctgcaa accgatgctt ccattaacca cggtaactct ggcggcgcgc
    56941 tggtgaactc gctgggcgaa ctgatgggca ttaacacgct gtcgtttgat aagagtaacg
    57001 atggcgaaac gccggaaggt atcggctttg cgattccttt ccagttagca accaaaatta
    57061 tggataagct gatccgcgat ggtcgcgtga tccgcggcta cattggcatt ggcggacgtg
    57121 agatcgcacc gctgcacgca cagggcggtg gtatagatca actgcaaggg atcgtggtta
    57181 atgaagtgtc acctgacggc ccggcggcga atgcgggtat tcaggtcaac gatctgatta
    57241 tttcggtgga taacaaaccg gccatctctg ctctggagac gatggatcag gtggcggaaa
    57301 ttcgccctgg ttcggtgatc ccggttgtag tgatgcgtga tgataagcag ttaacgctgc
    57361 aggtcaccat tcaggaatat ccggcaacca attaagtcga gtgctcaaaa caaaaaaccg
    57421 gagtctgtg
//
LOCUS       DAJTXQ010000009        55736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000009 DAJTXQ010000000
VERSION     DAJTXQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.009"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OO515_000529"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OO515_000529"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6574550.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO515_000530"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO515_000530"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6574551.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO515_000531"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO515_000531"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6574552.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO515_000532"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO515_000532"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6574553.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO515_000533"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO515_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6574554.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO515_000534"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO515_000534"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6574555.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OO515_000535"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OO515_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6574556.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO515_000536"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO515_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6574557.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OO515_000537"
     CDS             6537..6773
                     /locus_tag="OO515_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6574558.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OO515_000538"
     CDS             6776..7633
                     /locus_tag="OO515_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6574559.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OO515_000539"
     CDS             7654..9645
                     /locus_tag="OO515_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6574560.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO515_000540"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO515_000540"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6574561.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OO515_000541"
     CDS             complement(10248..11144)
                     /locus_tag="OO515_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6574562.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OO515_000542"
     CDS             complement(11193..11432)
                     /locus_tag="OO515_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6574563.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OO515_000543"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OO515_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6574564.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OO515_000544"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OO515_000544"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6574565.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OO515_000545"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OO515_000545"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6574566.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OO515_000546"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OO515_000546"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6574567.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OO515_000547"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OO515_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO515_000548"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO515_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6574568.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OO515_000549"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OO515_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6574569.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OO515_000550"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OO515_000550"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6574570.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OO515_000551"
     CDS             complement(21570..22739)
                     /locus_tag="OO515_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6574571.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO515_000552"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO515_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6574572.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OO515_000553"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OO515_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6574573.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OO515_000554"
     CDS             complement(24315..25247)
                     /locus_tag="OO515_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6574574.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPIHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OO515_000555"
     CDS             25360..26571
                     /locus_tag="OO515_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6574575.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OO515_000556"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OO515_000556"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6574576.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO515_000557"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO515_000557"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6574577.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OO515_000558"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OO515_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6574578.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OO515_000559"
     CDS             complement(30320..31576)
                     /locus_tag="OO515_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6574579.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OO515_000560"
     tRNA            31884..31960
                     /locus_tag="OO515_000560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OO515_000561"
     tRNA            31965..32041
                     /locus_tag="OO515_000561"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OO515_000562"
     CDS             32149..32454
                     /locus_tag="OO515_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6574580.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OO515_000563"
     CDS             32580..34184
                     /locus_tag="OO515_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6574581.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OO515_000564"
     CDS             complement(34196..35008)
                     /locus_tag="OO515_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574582.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OO515_000565"
     CDS             complement(35012..35797)
                     /locus_tag="OO515_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6574583.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OO515_000566"
     CDS             complement(35794..36462)
                     /locus_tag="OO515_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6574584.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OO515_000567"
     CDS             complement(36526..37164)
                     /locus_tag="OO515_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6574585.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="OO515_000568"
     CDS             complement(37177..39285)
                     /locus_tag="OO515_000568"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6574586.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="OO515_000569"
     CDS             complement(39306..39932)
                     /locus_tag="OO515_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6574587.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO515_000570"
     CDS             complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO515_000570"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6574588.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41154..42566
                     /gene="pykF"
                     /locus_tag="OO515_000571"
     CDS             41154..42566
                     /gene="pykF"
                     /locus_tag="OO515_000571"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6574589.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42876..43112
                     /gene="lpp"
                     /locus_tag="OO515_000572"
     CDS             42876..43112
                     /gene="lpp"
                     /locus_tag="OO515_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6574590.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO515_000573"
     CDS             complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO515_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6574591.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO515_000574"
     CDS             complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO515_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6574592.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO515_000575"
     CDS             complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO515_000575"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6574593.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO515_000576"
     CDS             complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO515_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6574594.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO515_000577"
     CDS             complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO515_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6574595.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO515_000578"
     CDS             complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO515_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6574596.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO515_000579"
     CDS             complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO515_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6574597.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50388..50576)
                     /locus_tag="OO515_000580"
     CDS             complement(50388..50576)
                     /locus_tag="OO515_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6574598.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO515_000581"
     CDS             complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO515_000581"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6574599.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51083..54139)
                     /locus_tag="OO515_000582"
     CDS             complement(51083..54139)
                     /locus_tag="OO515_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6574600.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54528..55640
                     /gene="ydiK"
                     /locus_tag="OO515_000583"
     CDS             54528..55640
                     /gene="ydiK"
                     /locus_tag="OO515_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6574601.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13420 a        14656 c        13638 g        14022 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtattggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaaa gcaagaaatt ataagtgaac tgatatttat tatcaattga
    40201 aataaattta acttaagcga caattatttg tgacgttcat tgcagaaaat aaaaataata
    40261 ttatcgttat agattaaaat acataccgca attcgtataa tttcagcagt tgttatttag
    40321 aatgattaac tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc
    40381 tgaatggtca ttttaccagt gcgttaatga cggtttccat cgcctgcttt tccagctcac
    40441 tgcgctgaat gcgtttattg gctaatgcag tacgtaatac gcctgcgata acaatatgct
    40501 ttggttcctg ccccagatcg cgcatttcca gcacaacttt accgacaact ctgcacattt
    40561 ctcggtacag ttcatcctct tttgtccgat ttcccattct ttatacctat ttatcatgcc
    40621 aactatcagc atatatcaat ctaaccaatt aaacaaaaat caaacaaaat cagacaaata
    40681 acgcgataaa ttattttaac tgtagcaatt gagcgatgat atatttatac accggatgaa
    40741 ctttcactta tcctcacact gacaacttcg gcaccagacg ttgcgcaaac agcgaagttt
    40801 ttgcgtaacc ttttccctgg aacgttaaat ctttgataac aatttattgt ctaacaagtt
    40861 gtatattttt tgaaacgctg tttttgtttt ccttttggat taatttcagc gtataatgcg
    40921 cgccaattga ctcttgaatg gtttcagcac tttggactgt agaactcaac gactcaaaaa
    40981 caggcactca cgttgggctg agacacaagc acacattcct ctgcacgctt tttcgatgtc
    41041 acctatcctt agagcaaggc accaccactt tcgtaatacc ggattcgctt tccggcagtg
    41101 cgcccagaaa gcaagtttct cccatccttc tcaacttaaa gactaagact gtcatgaaaa
    41161 agaccaaaat tgtttgcacc atcggaccga aaaccgaatc tgaagagatg ttagctaaaa
    41221 tgctggacgc tggcatgaac gttatgcgtc tgaacttctc tcatggtgac tatgcagaac
    41281 acggtcagcg cattcagaat ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta
    41341 tcctgcttga taccaaaggt ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg
    41401 tttctctgaa agcgggtcag acctttactt tcaccactga taaatctgtt atcggcaaca
    41461 gcgaaatggt tgcggtaacg tatgaaggtt tcactactga cctgtctgtt ggcaacaccg
    41521 tactggttga cgatggtctg atcggtatgg aagttactgc cattgaaggt aacaaagtta
    41581 tctgtaaagt gctgaacaac ggcgacctgg gcgaaaacaa aggtgtgaac ctgcctggcg
    41641 tttccattgc cctgccagca ctggctgaaa aagacaaaca ggacctgatc tttggttgcg
    41701 aacaaggcgt agactttgtt gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa
    41761 tccgtgagca cctgaaagcg cacggcggcg aaaacatcca catcatctcc aaaatcgaaa
    41821 accaggaagg cctcaacaac ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg
    41881 cgcgtggcga cctgggtgta gaaattccgg tagaagaagt tatcttcgcc cagaagatga
    41941 tgatcgaaaa atgtatccgt gcacgtaaag tcgttatcac tgcgacccag atgctggatt
    42001 ccatgatcaa aaacccacgc ccgactcgcg cagaagccgg tgacgttgca aacgccatcc
    42061 tcgacggtac tgacgcagtg atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg
    42121 aagcggtttc tatcatggcg accatctgcg aacgtaccga ccgcgtgatg aacagccgtc
    42181 tcgagttcaa caatgacaac cgtaaactgc gcattaccga agcggtatgc cgtggtgccg
    42241 ttgaaactgc tgaaaaactg gatgctccgc tgatcgtggt tgctacccag ggcggtaaat
    42301 ctgctcgcgc agtacgtaaa tacttcccgg atgccaccat actggcactg accaccaacg
    42361 aaaaaacggc tcatcagttg gtactgagca aaggcgttgt gccgcagctg gttaaagaga
    42421 tcacttctac tgatgatttc taccgtctgg gtaaagaact ggctctgcag agcggtctgg
    42481 cacacaaagg tgacgtcgta gttatggttt ctggtgcact ggtaccgagc ggcactacta
    42541 acaccgcatc tgttcacgtc ctgtaatatt gcttttgtga attaatttgt atatcgaagc
    42601 gccctgatgg gcgctttttt atttaatcga taaccagaag caataaaaaa tcaaatcgga
    42661 tttcactata taatctcact ttatctaaga tgaatccgat ggaagcatcc tgttttctct
    42721 cagttttttt atctaaaacc cagcgttcga tgcttctttg agcgaacgat caaaaataag
    42781 tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac ttgtaacgct
    42841 acatggagat taactcaatc tagagggtat taataatgaa agctactaaa ctggtactgg
    42901 gcgcggtaat cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc
    42961 agctgtcttc tgacgttcag actctgaacg ctaaagttga ccagctgagc aacgacgtga
    43021 acgcaatgcg ttccgacgtt caggctgcta aagatgacgc agctcgtgct aaccagcgtc
    43081 tggacaacat ggctactaaa taccgcaagt aatagtacct gtgaagtgaa aaatggcgca
    43141 cattgtgcgc catttttttg tctgctattt accactactg cgtcgcgcgt aacatattcc
    43201 cttgctctgg ttcaccattc tgcgctgact ctactgaagg cgcattgctg gctgcgggag
    43261 ttgctccact gctcaccgaa accggatacc ctgcccgacg atacaacgct ttatcgacta
    43321 acttctgatc tacagcctta ttgtctttaa attgcgtaaa gcctgctggc agtgtgtatg
    43381 gcattgtctg aacgttttgc tgttcttctg ctgatagtgg tcgatgtact tcaacataac
    43441 gcatcccgtt aggttccacg gaatatttca ccggttcgtt gatcactttc accggcgttc
    43501 ccgtccgcac gctggagaac aaggctttaa tatccggtgc attcatgcga atacaccctg
    43561 aactgacgcg caaaccaacg ctgtccggcg cactggtgcc atgaatgagg tattcgccat
    43621 taccatgcgc gaggcgcagt gcgtaacgtc ctagcgggtt atttggtccg gcaggaacga
    43681 ctggcggtaa tttaatgcca cgctccagcg aacgctgacg aatgcctgcc gtaggcgtcc
    43741 aggttgggtt agggattttc tgcccaacac gcgtttccat caccggcgtt tccagcccct
    43801 gcaatccaat acctattgga taaacctgca caatattttc tcccggcgga taataataaa
    43861 ggcgcagctc tgcaaggtta acgataatcc cctgacgcgg tgcatcaggt aataacagtt
    43921 gtgaaggaat agttatcgtc gtaccaggtt ttggcaccgg gacgatagtg ttattggctt
    43981 caaggatcaa cattgccgca gtatcaaaac gtcgggcaat agcctgaagg tttttatccc
    44041 cttcttgcac cgtatacgtt tgattttgcc caaccagtcg gcttccggtt ggtggtagcg
    44101 gataatcaac cgcccaggca gcctggatgg cgctaaaagc gccgataagc gtgagtgtaa
    44161 gcaaagacgc gcgtttcatt gtaaacctcc tgtatttgcc ggagactcac gctgaaacgt
    44221 cggatggcgc ttatgttcac ctgaaaccaa aacactcctg tgcaggtcag tgtaaacatt
    44281 gaccatccgg caatgtgagc caaccggatg aaagctgtcc ctttagttta gctaagtgca
    44341 gcggctttgg cgcgaattgc gcgaatcatc gcttccagac cttgtgaacg agatggggtg
    44401 agatgttggg tgagtgccat tttttcaaac cacggacgca catcgaaact gacaatatcc
    44461 tgcggcgtca tctgatcgta gagaataaag actaccgcaa taagcccttt cacaatcgcc
    44521 gcatcgctgt cgccctgcaa ttcaataatt ccgtgcgcat tctggcgcat gacaatccac
    44581 acctgactct ggcagccctg aatgctattt tgtgggcttc ggtcttcgtc gcgtaattca
    44641 gtcagacgct ggcccagctc aataatgtag agatatttct cttcccagtt ggcgcagcgt
    44701 aaaaaattac gcagcaattt ttctttatcc ggcaataaag ccatagtgcc tccctgttat
    44761 cccagcaaac ggtgaatacg ttgcaggccg gtcaccagac gatccacttc ttcatgggtg
    44821 ttatacatgg ccagcgacgc ccgacacatc gcagggacgt tgtaataggc cattaatggc
    44881 atagcgcagt gatgtccggt acgcacagca atgccgtaat tatcgagaaa actgccaaca
    44941 tcataggcgt ggtgtttacc gagattaaaa gcaataacgc caagcctgtt ttgtgggcca
    45001 tagagagtga gatccggtac agattccagc tgtgatagcg cataatgcat cagattctgt
    45061 tcatactcgg ctatgttatt aagccccagc gccgaaacat actccagcgc cgcgccaaga
    45121 ccaataatgc ccccggtatt gggtgtaccg gcttcaaacc gccatggtgc tttggtccag
    45181 gtagtgcctt cactcaggct gacggtggcg atcatagaac cgcccccttc ccacggcggc
    45241 atctcctgca acaaggcttc tttcacataa agaatgccaa ttccggtggg gccatacagt
    45301 ttatgcccgg agaacacgta aaagtcgcaa tccagcgcct gaacatccac cggatgatgc
    45361 atcaccgcct gagcgccatc caccagcact tttgcgccat gctggtgcgc aagcgtgatt
    45421 atttccgcca gcggattttc tgttcccagc acgttggaga catgagtaat tgccagcagg
    45481 cgagttttct catcaaacag cgtaggcagc gtctccagtt gcagcgtacc gtcggggttg
    45541 agagggatca cacgcagctc tgcgccaacg cgtgcgcaaa gcatctgcca gggaacaatg
    45601 ttagcgtggt gctccatctg actgatgatg atgttatcgc ccgcccgcac gttgctgttg
    45661 ccccaactat tggcgaccag attgatccct tccgtcgtgc cgcggacgaa caccagctct
    45721 tccgccgaac gggcattaat aaacagcgat gcccgcttgc gcacgttctc cattttctcg
    45781 gtcgcctggg cgcttaaggt atgaataccg cgatgcaccg ccgcgtagcc gtgacgataa
    45841 aactcagcct cggcgtcaat cacctggctt ggtttctgcg cactggcggc gctatcgaga
    45901 taagccagcg gcaaaccgtt cacctcacga gaaagcaccg gaaagtcggc ccgcactttg
    45961 tcgacggaaa aagtcatctt gcacctcctg gcagccgttg accgattcgg gccagcacct
    46021 gctgtttaag cccctcatca cgcagtgctt ccgtcagttc ggcagcaaag gcgtaaatga
    46081 tcatctgctg ggcatcctgc tgattgatcc cgcgcgagcg cagatagaac atctgttcat
    46141 catcaatacg ccccaccgtc gcgccgtggc tgcatttcac atcatctgca tagatttcca
    46201 gctgcggttt tgtatccact tccgccattt tgcccatcag cagattattg ttggtcatct
    46261 gaccatccgt tttgatggcg tgctgcgcga cgttgatcaa accgttaaat accgcgcggc
    46321 ctttgtcgct gacgatagtt ttgtgcaact gtcggctgtt acaaaaacct ttattgtgtt
    46381 ccagccaggt acgggtatca cacacctcgt ttttcaccgg catcgccagg ctattgatcc
    46441 gcagcgtgct gttttcgcca ttgagttgcg tactggtgtt gtgtcgtaac actgcgccac
    46501 ccagcaggaa actatggcta aatgcggtgg catcctcagc cagcaacaaa tcgttatgag
    46561 caaagtggtg actgagcggg ttttcaaacg ccagcttgat atgctgcaag tgggcgttcg
    46621 ctgcgacatt gatagtgaac cgtgccccgg taaaatgacg agcatcatta aggctgacaa
    46681 aatgttcgat caccgttgct tcggcacctt ccgccagatc cagatgatgc cggtaatggg
    46741 cagtgttcac ctcttcacct gccacgccct gggtgatatg cattaacagc aatggctttg
    46801 ccggccgttg accgcgcttc acggcgatat gcgtcacgct ttgtgccagg ctttccgtca
    46861 aatgcagaaa cacttccgcc tgaatagcgt cgggtaaccc ctgacggtcg tcgttaatgc
    46921 tcacttcata tccgctgcct tcaattgcat cgctcagcgc gggcacgtaa cgtccatcga
    46981 caaacaccag ccgcacggcg tctaacgtta acgctaaggc atcacgctgc tgtggggata
    47041 tatctcccgc aatgctgaca aactgactat tggtcagccc ttccagcggc gtatatttcc
    47101 agttttcatg tttacgtgtc ggcagtccgg tacgcagcaa ttgttgtaaa tgctgctgtg
    47161 cttgcggtga gcgttttgtc ccttcagctt caaacaagtg atgccactgt tgcagcgcat
    47221 tactgctgtt cggtaagcca gccataaccc tgctcctcca gttgtttgac caacgtgaaa
    47281 tcgccggatt tcacaattcg tccctgatat agcacatgga cgtaatcagg cttgatgtag
    47341 tcgagaatgc gttggtagtg cgtaacaatg atgaatgagc gcttgccgtc acgcagcgag
    47401 ttcacgccat cggcaaccac ttttaatgcg tcaatatcca gcccggagtc cgattcatca
    47461 agaatgcata actccggttc cagcaccgcc atttgcaaaa tatcgttgcg ctttttctcg
    47521 ccgccggaaa aaccaacgtt taccgaacgg gttaataaat cttccggcat cttcaggaga
    47581 gcgattttct cttccatcaa atcctgaaaa tcaaagcggt cgagcgtttc ctggccgcga
    47641 tagctgcgca ccgcattaag tgccgtttgc aggaaaaact ggttactgac gccgggaatc
    47701 tccaccggat actggaaggc cataaagatg ccttcgcctg cacgatcttc cggcgacagc
    47761 gcaagcaaat ctttgccttt gaactcaacc gtgccgcccg tcacttcata atcttctcgc
    47821 ccggcaagcg ttgccgataa ggtacttttg cccgaaccgt ttggccccat aatggcgtga
    47881 acttcgccgg gacgaacgtc gaggcttaat ccacgcagga tagctttatc ttccacgctg
    47941 acgtgtaaat ctttaatact taacatgttt attccttatc cgacgctgtg ttcaagactg
    48001 atggcgagga gtttttgtgc ttcaacggca aattccagcg gcagttctga gaacacgtct
    48061 ttgcagaaac cgttaacaat catcgagatg gcgtcttctt cgctaatccc gcgttgcagg
    48121 cagtaaaaca gttgatcttc accaatacgt gatgtcgttg cctcgtgctc cagttgtgcg
    48181 ctgttattgc gacattcgac atacgggaag gtatgcgccc cacaattagc gccaatcagc
    48241 attgagtcgc actgagtgaa attgcgcgca ttggttgccg tcggcatgat tttcactaag
    48301 ccgcgataac tgttctgact atgtccggca gagatccctt tcgagataat ggtcgatttg
    48361 gtgtttttac cgatgtggat catcttggtg ccggtatccg cttgctgatg accgctggtc
    48421 agcgccactg agtaaaactc accaatggag ttatcgccgc gcaaaatgca gctgggatat
    48481 ttccacgtaa tcgctgaccc ggtttctgat tgcgtccatg acattttgct gttttcgcct
    48541 tcgcacaaag cacgcttggt gacgaaattg agaataccgc cggtgttgtt atcgccaggg
    48601 aaccagtttt gtaccgtgga atatttcacc tcggcgtttt tatggatgat gacttccacc
    48661 accgccgcgt gtaactgata gctgtcacgc accggagcgg aacagccttc aatgtagctg
    48721 acgtagctgt cttcgtcggc caccagaatg gtgcgctcaa actgcccggt tttttccgcg
    48781 ttaatgcgaa aataggtgga aagttccatc gggcagcgca cgcctttagg cacataaata
    48841 aacgtaccgt cagaggctac cgccgcatta agcgcggcaa agaagttgtc atttcccggc
    48901 accacagtgc cgagatattt acgcaccagt tccggatgat cgtggatcgc ctcaccaaag
    48961 gaacagaaaa taattccctg ctccgccagt ttttcgcgat aagtagtggc aaccgaaaca
    49021 gagtcgaaaa tggcatccac cgccacctct ttgccttccc gcacgggaac gcccaactgc
    49081 tcaaacgccg cctccacctc tttacttaaa aaggcgttcg cgccagtttg ctgcaccgcg
    49141 ccaggttcag acgcgcaagt gtcgtcacaa ttaccgcacg atggtgctga gtagtaactg
    49201 taatcctgat aattcagctt gtcgtagtgc gctttcaacc agtgcggttc ttccatctcc
    49261 agccatgcgc gataggcgtt tagacgaaac tccagcatcc actccggctc attacgcttc
    49321 gccgaaattg cgcgcaccac ctcttcgttt atcccctttg ccagctcatc ggtggctaac
    49381 tgggtgaaga atccttcttt ataattcagc gggccgccgg tccaggtttt gacatcgtca
    49441 gttgcttcag tattacgaga catagtaccg cctatacccc aaagctttcg ccacagccac
    49501 attcattctg ggctttaggg ttgtgaaatt tgaatatctg attaagtcct tcacgaacga
    49561 aatcgacttc cgtgccatca ataaacggca tcgcttgcag cgggacaaac agcttcgcgc
    49621 cgtcgtgttc aaacagcaga tcgtctttgt ccggctcgct aacactgtcg agcacatagc
    49681 caaagcccgc gcagcccgtt tgcttcacgc ctaagcgcac gccgaccata cccggctgct
    49741 ttgccaccag ctcacggatg tgtatcgccg ctgcgggtgt cagcgttaag ccttgccagg
    49801 cgaaatcttg tgggttaaag gttcctgaat gcatgtccat cgatttacct cacttcatcg
    49861 ctttcagcgt ataacagcat gctagtgata atgattatca gttcaaccca gcaaacgcag
    49921 gggctttacc gtaaaacatg ctttttgcct gcttttaata agcatagacc ctgatgtgtg
    49981 ggttaacagg cacgctaaga attaggtatc tcattgttag ataatggtta ttatctaagg
    50041 agcattaagg ctgtgatgaa aaagaaaaag ttgtattgaa aatgactatt taagagatag
    50101 gtaaaaaagt gcagcgttca gaaataagaa aacccttaag tctgtgcgac acaggcttaa
    50161 gggtttctac cccatccggc gcttatctcc ggcactctca gtggcttagc tcttgaaggg
    50221 gcgataagaa taatctcata aagctaaccc gccgttttaa cacaaactgc gattagtatt
    50281 atttttgaac aatatcaggc ggtagataag cagtattaag aaggtcatcg aacctggacg
    50341 gaggttaatc caggtcgatt tggcgaactt gcggcattaa gtcaggatca atgcacgccc
    50401 agacgccagg caaagtagat ttcttctttt aattcagtag aagagagagt aagcaggtca
    50461 gcaaattcaa gttctagttg tttcagacgt ttgagatatt gggcaggtga aagattgctc
    50521 tggtcacggc gtaaaaattc aatggccagc tgggtgggat caagttgagt agacatagca
    50581 tcctcgcttt tagacaagac gtgcacagta taccaccgtt tactgtgcag ataatgacca
    50641 aaagcaatat gcgtcacact tttctggtga caacgtcaca aaatggcggt cgtcaatcgt
    50701 gacgaacagc acaaacgccc tttctcatcg aagatttcaa tctgccagac ctggtgacgc
    50761 gaaccgagat gcaacggttt acatatgccg cgcacccgcc cttctcgtgc cgagcggacg
    50821 tgattagcat tgatttccag accaaccact ttttgctcac cttcggtaca taaataaccg
    50881 gcaacggaac cgatactttc ggccagtacc acggatgctc ctccatgcag caacccgaaa
    50941 ggctgctttg tccgcgagtc tactggcatt gtcgcttcaa gggtgtcatc accaatatgt
    51001 tcaaagcgaa tatccaggaa ccccaccatg tttccttcac ccatagcatt cagtgcttcc
    51061 agggtgattt tccgtttcca tatcatttaa taatctccag taaagcctgc acaggatggc
    51121 gtacccccgt gccttcaacc cgttttacct ggctacggca ggaatatccg gtcgccagac
    51181 agcggttacg cggcagtcgc tgcatagcct gatgccagga taactcatag atcccgagcg
    51241 aattttcatg gtttttcgct tcatgtccgt aagtccctgc catgccgcag caacccacgc
    51301 tgacattttc cagtttcgcg ccaaaacggg caaatatcgc ggcccattgt gctggcgcgc
    51361 ccggcaaagc ggtaacctcg gtacagtgac caaagaaata ccatgattca ccgctgactg
    51421 tagccactgg atgtgactca agtgcgcttg ccagccattc attcgcaagt aagacgttaa
    51481 actcgccacg ttcctcgccc agcgccagtt tatattcatc gcgataacaa agtaccagcg
    51541 ccggatcgac gcccaccatt ggcatcccaa gcttagccat acggttgagg aaatccgccg
    51601 tctttttcgc cgtcttcgca aaacgattaa gaaagccttt gatatgctgg gctttgccat
    51661 ttggcgaaaa tggcagtaac acaggctgga aacctaattt ttcaaccaga cggacaaaat
    51721 ccgccaccac ttgcgcatcg taatagctgg taaaggggtc ctgcaccacc aacaccgtgc
    51781 gcgctttctg ctctgcattg agagcttcaa gctgttccag cgtcatgttt gccgagcgat
    51841 gccccaccat ttgttgttgt agcgagggga ccgacagcag tggcaaatca accatgccga
    51901 tatgtttttc cgagagtttg cgcaccagcg gctggttaat gaagaagtta aaggttttcg
    51961 gcgcgcgtgc catcagcggt gcgtagctct cgaccgtagc gacgaggtgg tcgcgcagcg
    52021 ggcgtaaata acgggtgtga tagagctgca ggaaacgaga gcgaaactcc ggcacatcaa
    52081 ttttgatggg gcactgggtc gaacacgctt tacaggccag acagcccgac atcgcctctt
    52141 tgacttcgtg tgagaagtca tattcgcctt tattcgcatg ccagctattg cgcgtgcggg
    52201 caattaacgt ccgtaaactg acgccggatt caggcagttc ttgttccagt ttgagtggat
    52261 caacgccgcg atccgccaac aaacgcagcc attcacgcac cagcgttgcg cgccctttcg
    52321 gtgaatgaat ccggttctgg gtgatcttca tcgacggaca catcggacta cgggcatcaa
    52381 agttgaagca taaaccgttg ccgttacact ccatcgcacc gcgccactgc tggcgtaccg
    52441 caatggggat ctgccgatcg aatgtaccgc gcttcaccgc gtccactttc atcatcggcg
    52501 catcgagacc ttctggcggg caaatcttcc ctgggttgag tcggttatgc gggtcaaatg
    52561 ccgctttcac tttgcgcagt tctgcaaaaa gttcctcacc gaaaaacgcc gggctgtatt
    52621 cagcgcgaaa acctttgccg tgctcgcccc acaacaaacc accgtatttc gcagtcagcg
    52681 ccaccacgtc atcagagatt tgcttcatca aaatctcttg ttgcggatcg cacatatcca
    52741 gtgccggacg gacgtgcaaa acacctgcgt cgacgtgacc gaacataccg tagcttaagc
    52801 cgtggctgtc gagcagcgcg cgaaattcag caatataatc ggccagatgt tccggcggta
    52861 cgcaggtatc ctcagcaaac ggaattggct tagcggcacc tttggcattg ccaagcagac
    52921 caacggcttt tttgcgcatt gcatagatac gttcaacccc cgccagctcg cggcacacct
    52981 gccagccaat cacacctgcc tgttggctgg caatcagctc atcaagccgc acacagagtg
    53041 cattaactcg ctcgtcaatc agcgcctcat catcaccagc aaattccaca atgttcagcc
    53101 cgagcatctc tttgtcaggc acatcggtaa tcaactcgct gacggaatgc cagacaatat
    53161 cttcccgcgc cagattcagc acttttgagt ccaccgtctc taccgaaagc gcccgcgcct
    53221 caaccataaa cggcgcgtta cgcagcgcgg agtcaaaaga gtcatatttg acgttcacca
    53281 gacggcgcac tttaggcaag ggcgtaatat ccagccgcgc ttcggtaata aaggccagcg
    53341 tcccttctga acccgtcaga atgcgcgtca ggtcgaactc ggtcatctca tcgttaaaga
    53401 catgacgcag atcgtaaccg gtaagaaagc ggttaagttt ggggaagttg tcgatgatta
    53461 actggcgttg cagacggcaa cgttgataaa ccgtgttata aattcgcccg attgtggtat
    53521 tggttttacc cagcgtttcc gccagttcga cgggtaaagg ttgcgtatcg agaatatcgc
    53581 cccccaacaa caccgcgcgt acgccaagta cgtgatctga cgttttgcca tagaccagcg
    53641 atccctgacc ggatgcatcg gtattgatca tcccgccgag cgttgcccgg ttgctggtcg
    53701 aaagttctgg tgcaaaaaag tagccgaacg gtttcaggta ctgattgagt tgatctttta
    53761 tcaccccggc ctcaacgcgc acccagccct cttcagggtt aatttcgatg atgcggttca
    53821 tatggcggga catatcaaca ataatcccct ggttgagcgc ctgaccgtta gtgccggtgc
    53881 cgccgccgcg gggggtaaag atcagcgatg aatagcgttc ctgcgcggca agacgggcga
    53941 tcagcgccac atctgcggtt gaacgcggaa ataccaccgc atcggggaga agttggtaaa
    54001 tactgttgtc ggtcgacatt gtcagacgat cggcataact tgtcgccgta tcgccggtaa
    54061 aaccttgttg ctccagctct tgcaaaaaat taagcaccag ttgaacgacg ccgggtgcct
    54121 gggaaatctg tggaatcatt atattgaccc tttcctgcgg tctgtgatgt aggtcgatac
    54181 actattcttt caggcggctg caatagcgca ctgaaaggtg atgtttattt actctatgga
    54241 tttcgagttg caggaaggcg gcaagcgagt gaactccagg agcttacaat agtaagtgac
    54301 tggggtgaac gaacgtagcc gcagcgcatg caacttgaaa tacgacgagt aaatcgtttg
    54361 cgtgttgcct gagttgttgt accacatttt tttctaacac gcccatcaga attaagggca
    54421 gaatcggcct gttaaaaacc gctgaaattg ctcatcatta tgcaggtgag tttcgcgtgt
    54481 tcacgtcgcg tcgacgattt gacgcacaaa aaaggtgaaa agtagttatg gtaaatgttc
    54541 gtcagcccag ggatgtcgca caaattctgc tttcggtgct gtttttagcc atcatgattg
    54601 tggcatgtct gtggattgtt caacccttta ttctcggctt tgcatgggcc ggtacggtgg
    54661 ttatcgccac ctggccggta ttgttacgtt tgcaaaagat catgtttggc cgccgctctc
    54721 tcgccgttct ggtgatgacg ctgttattag tgatggtgtt tatcatccct attgctttgc
    54781 tggttaacag tatcgtcgac ggcagcggcc cgctaattaa agccatttcc agcggcgaca
    54841 tgacgttacc cgatctggcg tggcttaata ccattccggt gattggcgcg aagctgtatg
    54901 caggctggca caacttgctg gatatggggg gtacggcgat catggcgaaa gtccgccctt
    54961 atattggcac caccaccacc tggttcgttg ggcaggcggc gcatatcggg cgctttatgg
    55021 tgcattgtgc gctgatgctt ctcttcagtg ccctgctgta ctggcgcggt gaacaggtgg
    55081 cacaaggcat tcgccatttt gcaacccgtc tggcaggcgt tcgcggagat gccgccgtgc
    55141 tgctggcggc acaggctatc cgcgcggtgg cgctgggtgt ggtggtgacg gcgttagtac
    55201 aggcagtgct tggcagtatc ggcctcgccg tatccggcgt accttatgca acgttgctaa
    55261 cggtgttaat gatcctctcc tgccttgtcc agcttggccc gttgccggta ctgattccgg
    55321 cgattatctg gctctactgg accggcgata ccacctgggg aacggtattg ttagtgtgga
    55381 gcggtgtggt tggcacgctg gataacgtca tccgcccaat gttaattcgc atgggtgccg
    55441 atttaccgct gatcctgatc ctctctggcg ttattggtgg tttgattgct ttcgggatga
    55501 tcggtctgtt tattggtccg gttctgttag ccgtttcctg gcgtctgttt gccgcgtggg
    55561 tggaagaagt cccgccgccg acggaccaac cggaagaaat tctcgaagaa cttggcgaaa
    55621 tcgagaaacc gaataagtaa tttctcatca ggcggctctg ccgcctgatt gttaatcacc
    55681 gctaattgat atttgcttgc tcttccccat attttcctgc ttaccgccat tcagct
//
LOCUS       DAJTXQ010000010        50054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000010 DAJTXQ010000000
VERSION     DAJTXQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.010"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..1274
                     /locus_tag="OO515_000584"
                     /pseudo
     CDS             54..1274
                     /locus_tag="OO515_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="OO515_000585"
     CDS             1247..1876
                     /locus_tag="OO515_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6574602.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO515_000586"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO515_000586"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6574603.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="OO515_000587"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="OO515_000587"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6574604.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="OO515_000588"
     CDS             complement(4244..4441)
                     /locus_tag="OO515_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6574605.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO515_000589"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO515_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6574606.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO515_000590"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO515_000590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6574607.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO515_000591"
     CDS             complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO515_000591"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6574608.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO515_000592"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO515_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6574609.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO515_000593"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO515_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6574610.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO515_000594"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO515_000594"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6574611.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="OO515_000595"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="OO515_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6574612.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO515_000596"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO515_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6574613.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="OO515_000597"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="OO515_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6574614.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="OO515_000598"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="OO515_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6574615.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="OO515_000599"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="OO515_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6574616.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO515_000600"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO515_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6574617.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO515_000601"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO515_000601"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6574618.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO515_000602"
     CDS             complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO515_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6574619.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO515_000603"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO515_000603"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6574620.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="OO515_000604"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="OO515_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6574621.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="OO515_000605"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="OO515_000605"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6574622.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO515_000606"
     CDS             complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO515_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6574623.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO515_000607"
     CDS             complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO515_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6574624.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28466..29584
                     /locus_tag="OO515_000608"
     CDS             28466..29584
                     /locus_tag="OO515_000608"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6574625.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29650..29898
                     /locus_tag="OO515_000609"
                     /pseudo
     CDS             29650..29898
                     /locus_tag="OO515_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            29963..30331
                     /locus_tag="OO515_000610"
     CDS             29963..30331
                     /locus_tag="OO515_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6574626.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30425..31078
                     /gene="nfsB"
                     /locus_tag="OO515_000611"
     CDS             30425..31078
                     /gene="nfsB"
                     /locus_tag="OO515_000611"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6574627.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31186..32433
                     /gene="ybdG"
                     /locus_tag="OO515_000612"
     CDS             31186..32433
                     /gene="ybdG"
                     /locus_tag="OO515_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6574628.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO515_000613"
     CDS             complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO515_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6574629.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO515_000614"
     CDS             complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO515_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6574630.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO515_000615"
     CDS             complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO515_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6574631.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO515_000616"
     CDS             complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO515_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6574632.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO515_000617"
     CDS             complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO515_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6574633.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40268..40951
                     /gene="cusR"
                     /locus_tag="OO515_000618"
     CDS             40268..40951
                     /gene="cusR"
                     /locus_tag="OO515_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6574634.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40941..42389
                     /gene="cusS"
                     /locus_tag="OO515_000619"
     CDS             40941..42389
                     /gene="cusS"
                     /locus_tag="OO515_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6574635.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            43126..45027
                     /locus_tag="OO515_000620"
     CDS             43126..45027
                     /locus_tag="OO515_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6574636.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            45055..45516
                     /locus_tag="OO515_000621"
     CDS             45055..45516
                     /locus_tag="OO515_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6574637.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            45536..49947
                     /locus_tag="OO515_000622"
                     /pseudo
     CDS             45536..49947
                     /locus_tag="OO515_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        12013 a        13761 c        13091 g        11189 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata
    27601 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    27661 tcaggcaccc gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa
    27721 gtcctgtttc ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc
    27781 agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa
    27841 ccggatatgc ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    27901 agccttaaac tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag
    27961 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt
    28021 cagcatgttt atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg
    28081 gagtggcagc cccagggttg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28141 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcaacaaa
    28201 atggctgttt atttatacag taaatcctgt ttcggacttc tcactcctca tttaatcaac
    28261 tgaagctgag cccgtttttt ctgcatgcct caatcgtcaa aattcttcgc catcagccag
    28321 ctctgccata acttgccaca ttgaaaaagt agccaaattg cttcagtcac catcatcaaa
    28381 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
    28441 gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
    28501 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
    28561 ggactcttca atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca
    28621 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    28681 tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    28741 ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    28801 taacgagcgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    28861 ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca
    28921 cggcttgtca cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca
    28981 aggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa
    29041 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtttgag
    29101 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    29161 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    29221 tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc cgttcaaaca
    29281 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct
    29341 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagacacctt
    29401 gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga
    29461 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    29521 cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccgatga
    29581 ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    29641 aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg
    29701 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    29761 ctggtgacca tgttccatct gatggatctt agctagcttt acactttatt gttttgtctg
    29821 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    29881 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    29941 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    30001 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    30061 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    30121 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    30181 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    30241 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    30301 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    30361 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    30421 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    30481 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    30541 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    30601 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    30661 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    30721 tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
    30781 aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat
    30841 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg ctctgggtct
    30901 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    30961 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    31021 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    31081 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    31141 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    31201 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    31261 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    31321 cggaccttcg aaaaacgagc catcgccagt tcacggcttt ggttgcaaat cataacccag
    31381 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31441 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31501 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31561 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31621 ctgatcggcg cgatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31681 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    31741 ccgattcttg gtctggtggc cggtattcag ctttccgcga acgatatgct gaaactgggc
    31801 gactggctgg agatgccgaa atacggcgca gatggcgcgg tgatcgatat tgggttaacc
    31861 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31921 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    31981 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    32041 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
    32101 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
    32161 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    32221 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    32281 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    32341 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    32401 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32461 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32521 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32581 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcag agatagtttc
    32641 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac
    32701 gaaaacgcag atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32761 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    32821 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    32881 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    32941 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    33001 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    33061 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    33121 tgcgatacac cacctgattt accgcttttg gaatgctttt atccggatcg cgcgcttcag
    33181 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    33241 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    33301 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    33361 tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt
    33421 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33481 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33541 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33601 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct
    33661 ggcgcataat caggaaagcg atgatcccgg cgacgccgta acccagcaat acagccggac
    33721 ccgccatctg aatcgccgga ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33781 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    33841 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33901 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    33961 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    34021 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    34081 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    34141 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg
    34201 atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc
    34261 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc
    34321 ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag
    34381 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34441 cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat
    34501 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34561 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    34621 caccatgtca cgatcgcggg catcaatata aatccagctc gtcgggcgcg cattctcggt
    34681 tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg
    34741 ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct
    34801 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    34861 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34921 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    34981 tagagcagaa gccacgcttg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    35041 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    35101 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    35161 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg
    35221 atcctgcggc ttaagctgga tggtcgtttc aaccatctcg agcggagcag aatcggtggc
    35281 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    35341 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    35401 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35461 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    35521 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35581 actgctttcc ggcggaattt tgccacggat ccagtacccc atcaggatag ggatcactac
    35641 gatcgccagc agcgccgcgc ccgccatcgc ataggtttta gtgaacgcca acgggccaaa
    35701 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag
    35761 cagactgata aatagcgccg gacccacttc aacagatgca tcggtgatca cctgccagcg
    35821 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35881 tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    35941 gcccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca
    36001 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    36061 cgcacagact accgccacca cgataaactc ttccagcaac ttgccgctga ggttgtcgat
    36121 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    36181 actacttttc agcgtttcca gtttgtcctt cacggaggcg atcacttctc gggcgttttt
    36241 gccggagcgc aggatcacca ccccgcccgc cacttcgcct tcgccattca gttcggcaat
    36301 gccccggcgc atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac
    36361 gccattttct ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36421 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36481 gtttgaagca tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg
    36541 ctggggatcg ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc
    36601 aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
    36661 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36721 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36781 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac
    36841 atacacataa gagtctccaa actgcgagaa accgcgcaca gtcttcgcgc caggcaccga
    36901 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc
    36961 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg
    37021 cgtgttaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
    37081 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    37141 gcattggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    37201 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagtgtaac
    37261 gcggtgacgc cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgtccgtcg
    37321 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg
    37381 agcagcatcg gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc
    37441 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37501 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg
    37561 agcgtaaact gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg
    37621 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37681 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag cgtaaagcga
    37741 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37801 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37861 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37921 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    37981 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    38041 aaagtcagcg gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    38101 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    38161 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    38221 taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt
    38281 gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata
    38341 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    38401 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca
    38461 ctcattttcg tctgcggggt gatggtaaag cgcatagtca tctccggcca gttcacggca
    38521 gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg
    38581 ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta
    38641 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    38701 ttcatttatt ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat
    38761 acaaagaaat ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg
    38821 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    38881 atgcccgcgc ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga
    38941 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    39001 ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat
    39061 tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca
    39121 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    39181 tcaggctgat agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca
    39241 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    39301 ccgccggtaa tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt
    39361 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    39421 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    39481 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    39541 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    39601 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    39661 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    39721 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    39781 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    39841 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    39901 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt
    39961 tctggtagtt atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca
    40021 cgggcattgc cgtacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    40081 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    40141 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    40201 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    40261 aggaaaaatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    40321 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    40381 tctggcgatg accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    40441 cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct
    40501 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta
    40561 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    40621 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    40681 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    40741 gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    40801 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    40861 acgcggcaaa atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg
    40921 ctatatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    40981 cccgtctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    41041 tcatgatcca ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    41101 ttagcgccac ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    41161 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc
    41221 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    41281 ccatacccga taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta
    41341 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc
    41401 cggttggccc gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    41461 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca
    41521 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    41581 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    41641 cgcagaccga gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    41701 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    41761 gcacgccgat taccaatctc attacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    41821 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    41881 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    41941 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg
    42001 aagatcgcgg cgtggagttg cgatttgttg gcgacaaatg tcaggttgcg ggcgatccgc
    42061 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaacca
    42121 gagagacaat tgtagtgcgc tgtcagacgg tcgatcacca ggtgcaagtt accgtcgaaa
    42181 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    42241 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    42301 cgattgttgt cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    42361 ttgtgattat gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat
    42421 cacctcctgg tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc
    42481 gaaggctttt atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg
    42541 tgtttcttcc aattgtagat cacaatgccc tttctaaatt ttcatcgtta taaacaagcc
    42601 atctatcata taaagcaatg aataatttaa tcgcgattaa tcatcattaa ttgttatatt
    42661 attcactcaa tctatttgtc aagtgaattt gataaattcc taatttaaaa tatccactat
    42721 tgagccagta tttaacaacg ttaagatgtg caatgtgcag atcatgttat cagggtggag
    42781 cggtagcata tatttcttat gcaatcattc attttaataa tgttaattgg attatttatt
    42841 gctgtgaata aaaatatatt atgaatattg gtttaacttt gcgtttttgt tactcgttgt
    42901 gatatgcgta cctgtcctga ataacgccaa gaaacaagag gttgagcttg tgattatttt
    42961 tttgactgaa cagacacccg tttctgtaaa gtaatattta aacaaaattc tcacataacc
    43021 atagcatttt ctgttccttc cctaataacg tctgaagtcg tcccgcttat cacattatcc
    43081 gcagttgtac ggtacggatc attattcatt ttactggagg ttgctatgtc aaccggatta
    43141 cgtttcacac tggaagtgga cggcctgccg ccggacgctt ttgcggtggt ttcctttcat
    43201 ctgaaccagt cactctcttc gctcttttcc ctcgatctct ccctggtcag ccagcagttt
    43261 ctctcccttg aatttgcgca ggtgctggac aaaatggcct acctgacgat atggcagggc
    43321 gatgaagtac agcgccgggt gaaaggcgtg gtgacctggt ttgaactcgg ggagaacgac
    43381 aaaaaccaga tgctgtacag catgaaggtg cacccgccgc tgtggcgtgc cggtctgcgc
    43441 cagaacttcc gtatcttcca gaacgaggac atcaaaagca tcctcggcac gatgttgcag
    43501 gaaaacgggg tgaccgaatg gagtccgctg ttcagcgagc cgcatccttc ccgtgagttt
    43561 tgtgtccagt acggtgagac tgattacgat ttcctgtgcc ggatggcggc ggaggaaggc
    43621 atcttctttt atgaggagca tgcttacaaa agtaccgacc agagcctggt gctgtgcgac
    43681 acagtccgcc atctgcccga atcttttgaa atcccatgga acccgaacac ccgtaccgag
    43741 gtgagcaccc tctgcatcag ccagttccgc tacagcgcac aaatccgccc ttcttccgtg
    43801 gtgaccaaag actacacctt taaacgcccc ggctgggccg gacgttttga acaggaaggc
    43861 cagcaccagg actaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag
    43921 ggtgctcacg ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caatgcagaa
    43981 accgcgcggg gaatgagtcg ctcgccggag atatggccgg gacgacgaat tgtgctgacg
    44041 gggcatccgc aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc
    44101 gaacagccac aggcggtgcc aggacggcag ggagcgggga cggcgctgga gaaccatttt
    44161 gcggtgatcc cggcagacag aacatggcga ccacagccgt tgctgaaacc gctggtcgac
    44221 ggcccgcaga gcgctgtcgt gacaggaccg gcaggcgagg aaatcttctg cgacgaacat
    44281 ggtcgcgtgc gggtgaagtt caactgggac cgttataacc cggcagacca agacagttcg
    44341 tgctggatcc gtgtggcaca ggcgtgggca ggcaccggtt ttggccacct ggcgataccg
    44401 cgtgtgggtc aggaagtgat tgtagacttc ctcaacggcg atccggacca gccgatcatt
    44461 atggggcgca cctaccacca ggaaaaccgc ccccccggca gcctgccggg aacaaagacg
    44521 cagatgacca tccgctccaa aacgtacatg ggcagcggat ttaatgagct gaagtttgat
    44581 gatgcgacag ggagagaaca ggtctacatc cacgcgcaga agaacatgga taccgaagtg
    44641 ctcaacgacc gtaccaccac cgtaaaacac gatcaccgcg aaaccgtaaa aaatgaccag
    44701 acggtcacga tccaggaagg taaccgcctt cttacggtgg aaaaaggcca caagatcacc
    44761 ggagtactga aagggtcttt atctgaggat gtctttcagg acagaggcac gattgccggt
    44821 tcggtgcatg ttgacgctgt aaacaatggt ggcgaaggcg acggtataca ggcttatacg
    44881 gcgattaagg aaattttgct ggccgtggag gaaagcaaaa ttgcgctgac gccggatagc
    44941 attcagctac aggtcgggga atcgacggta atcaggctgt cgaaggatgg catcaccatc
    45001 gtgggcggtt ctgttttcat caactgattc actcagatta tcagggagta ttttatgcag
    45061 ttcactttta atgaaggcca cattcaactg ccgtctcaat ggcaggatca gtcaatgcag
    45121 gtcctggtgt ccacagataa cagtggcatc aacctggtca ttacccgcga agccgttcca
    45181 cagggaaccc tgacaccaga gctgtaccag gaaacgctgg cgctgtacca gggcaaactt
    45241 gatgggtaca ccgaacatgc ctgccgggaa atcactctgg cagaggcccc ggcctggctg
    45301 ctggattatt cctggaacgg tccggaagat gaggggaatc aggggcggat tagccagata
    45361 gccgtattcc agcggcgtgg agatacgctg ctgaccttca ctttcagcac ctccttatcc
    45421 ctgaagaaca gtcaaaagac gatgctgctg gaggtgataa agagcttcac cccgttgcca
    45481 ccggagaatg acatccaaaa ggaccagccg cgctgacata aacgagggat aacacatgag
    45541 tgaaggacca ggcgggccac agggagcgac cgcaggcggt acgctggcaa tgcgaatgct
    45601 gtcacagcag gcgatggtcg ccagccagat gaaacgggca gccaacgaca aagccattgc
    45661 acagatgttg gcatcaaaaa agtctggccc ccccgccgcc aggctgggcg atgaaattca
    45721 gcacaagagt tttttggggg cgctggcagg ggccgtactg ggggcgatag tgaccatcgc
    45781 agaaggttgc ctgattatgg ccgcctgtgc caccggccct tatgcgctgg ttctggtacc
    45841 tgcgctgatg tatgccagct ataaggcgag tgattatgtg gaggagaaac agaaccagct
    45901 tgaatcatgg ataaacagct tttgtgacac ggacggcgcc atcaataccg gttctgaaaa
    45961 cgtaaaaatt aacggcgagc tggccgcacg tgcagcggtc acccttcccc ctcctccccc
    46021 acctggagca atacctgaag tcccacaggg ggaaccctca tggggtgata ttgccactga
    46081 cctgcttgaa tcggcagcgg aaaaagcagt accactggcg aaggcctggg ggaacgctgt
    46141 tatcaccctg acggacagca atgccggttt tatggatcgc gtcagcgccg gcgcatcgct
    46201 tctgtttccc gccggtccgg tattaatgga gtttgccacc atggtgggcg ggcgtggcga
    46261 aatcaaaaaa gatgtggatt tcccggaagc cggtgaggac acggcgctct gcgacaagga
    46321 gaacaaacca ccgaggatag cccagggcag cagcaacgtc tttatcaaca atcagcctgc
    46381 cgcgcgcaag ggcgacaaac tggagtgcag tgcggcgatc gtgggaggtt cgccggacgt
    46441 ctttattggc ggtgagcagg tcacatatct ggatatccag ccggagttcc cgccgtggca
    46501 gagaatgatc ctggggggaa taacgatagc cagctatctt ctgccgccag cgggactact
    46561 gggaaaactg aagaatctgg cgagactcgg caaactggga aactgctggg gaaaagcggg
    46621 aagctgctgg gcgcaaagct cggcgcgttg ctggggaaaa caggtaagtc gttaaaaagt
    46681 attgccaata aagtcatcag atgggtaaca gatcctgtcg atccggtaac cggcgcatac
    46741 tgcgacgaac gtaccgactt caccctgggc cagaccctcc ccctctcctt cacccgtttc
    46801 cacagttctg tactgccgct gcatggcctg accggcgtgg gctggagcga ctcctggagc
    46861 gaatacgcct gggtgcgtga acagggaaac cgggtggata tcatcagcca gggagccacg
    46921 ctgagatttg ccttcgacgg tgacagtgat acggcggtta acccgtatca cgcccagtac
    46981 attctgcgcc gccgcgatga ttatctggag ctgttcgaca gggatgcact gagcagccgc
    47041 ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc cggtgactga cgataccagc
    47101 gatgaccgcc tggcacacag ccccaatgac cggatgtaca tgctgggcgg gatgagcgac
    47161 accgccagca accgcatcac gtttgagcgc gacagccagt accggatcac gggtgtcagt
    47221 cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct gaaagccatt
    47281 caccgcacgg ataacgacat acagacgctg gcgacctacg aacaggatgc gcgggggcgg
    47341 ctgacagaag cggatgcgcg gctggactac cacctgtttt atgagtacga cgctgcggac
    47401 cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta cgatgaacag
    47461 ggccggtgcg tgaatgtcac cggggcggag ggctattaca acgccacgct ggactatggt
    47521 gacggctgca ccaccgtgac ggacggcaag ggcattcacc gttattacta tgatcctgac
    47581 ggcaatattc tgcgggaaga agcgccggac ggcagcacca ccatgtatga atgggatgaa
    47641 ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaaatt tgaatacaac
    47701 gccgcacacg gtcagttaag ccgttacacg gcggcagacg gcgcggagtg gcagtaccgc
    47761 tatgatgagc gcggcctgct cagcaacatc accgaccctg ccgggcagac gtggacacag
    47821 cagtgtgatg aacgcggcct gccggtaagt ctggtgtcgc cacagggcga agagacccgg
    47881 ctggcataca ccgctcaggg gctgctgtcg ggaatattcc gccaggatga acggcgtctg
    47941 ggcatagagt acgaccacca caaccggccg gaaacactca ccgacgtgat gggccgtgaa
    48001 caccacaccg aatacagcgg tcacgacctg ccagtgaaga tgcgcggccc cggcggtcag
    48061 tcagtgcggt tacagtggca gcagcaccat aaactgagcg gcattgaacg tgctggaacc
    48121 ggcgcggaag gatttcgcta tgaccgccac ggcaacctgc tggcctggac ggacggcaac
    48181 ggtgttgtct ggacgatgga atacggtccg ttcgattttc cggtggcgcg aacggacggt
    48241 gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcaactgac agaagtcatc
    48301 aacccgcagg gtgagtcata tctttatgtt ctggataact gcggccgggt gacggaagag
    48361 cgtgactggg gcggcgtggt ctggcgttac cgctatgacg ccgatggcct ctgtaccgcc
    48421 aaggttaacg gcctggagga aaccatcctc tacagccggg acgccgcagg ccgcctggca
    48481 gaaatcatct cgccggaagg caaaacgcag tatgcctatg acaaatccgg caggctgacg
    48541 ggtatcttca gcccggacgg cacatcacag cgcaccggct atgacgaacg cgggcgggtg
    48601 aatgtcacca ctcagggccg acgggccatt gaatacaact accccgatga acacaccgtc
    48661 atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccggtga atccctgctg
    48721 aaaaccacgt accgctacaa cgccgccgga gaactgacag aggtcattct gccgggggat
    48781 gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca cagtaaccgg
    48841 ggttttgcct gtgaacaggg ctggaatgca gccggtcagc ctgtcagtca gcgcgccgga
    48901 ttttttccgg cagaagccac atgggacggg ctggttccct cactggtacg ggagtaccgt
    48961 tacgacagcg cgggtaacgt gtcaggcgtc accagccggg aagattacgg acgggaaaca
    49021 cggcgggagt atcggctgga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc
    49081 gggctgggct atggcgaagg cgatgagtcc tatggctatg acagctgcgg ctacctgaag
    49141 gcgcagtctg cgggcaggca ccggataagt gaagagactg accagtatgc cggaggccac
    49201 cggctgaaac aggccggaaa cacgcagtat gactatgacg ccgcaggccg gatggtcagc
    49261 cgcaccagac accgtgacgg ctaccgaccg gaaacagagc ggttccggtg ggacagccgg
    49321 gaccagctga ccgggtattg cagcgcacag ggtgagctgt gggaataccg ccacgacgcc
    49381 agcggcagac gaacagagaa acgctgcgac cggaagaaaa tccgcttcac gtatctgtgg
    49441 gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta cagcgtaagg
    49501 cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt acggcaggcg
    49561 catccgtcgg tggccccgca gtgggtgacg cggacgaatc atgcggtgag cgacctgacg
    49621 ggccgcccgc tgatgctctt taacagtgaa ggaaagacgg tctggcgacc ggggcagacc
    49681 agcctgtggg ggctggcact cagcctgccc gcagacaccg gctacccgga cccgcgcggg
    49741 gaactggacc cggaagccga ccccggcctg ctgtatgcag gacagtggca ggatgcggaa
    49801 tccgggctgt gctataatcg gttccggtat tacgagccgg aaaccggaat gtacctggtg
    49861 agtgatccgc tggggttgca gggcggggag cagacttacc ggtatgtgcc gaatccttgt
    49921 gggtgggttg atccgctggg attggctgca agttctaaaa tcagcagttt gatggactat
    49981 attggcgatg gtcgtcgtgt tagtgggcat acgggtttcc tggatggggt tcgtttatca
    50041 cgtagtcaaa taaa
//
LOCUS       DAJTXQ010000011        49943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000011 DAJTXQ010000000
VERSION     DAJTXQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.011"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            37..1191
                     /locus_tag="OO515_000623"
     CDS             37..1191
                     /locus_tag="OO515_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6574638.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            1342..2166
                     /locus_tag="OO515_000624"
     CDS             1342..2166
                     /locus_tag="OO515_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR6574639.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINNSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
     gene            2252..3454
                     /locus_tag="OO515_000625"
     CDS             2252..3454
                     /locus_tag="OO515_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCR6574640.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPNDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            3514..4098
                     /locus_tag="OO515_000626"
     CDS             3514..4098
                     /locus_tag="OO515_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6574641.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKDPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            4493..4951
                     /gene="traH"
                     /locus_tag="OO515_000627"
     CDS             4493..4951
                     /gene="traH"
                     /locus_tag="OO515_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HCR6574642.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            4948..5766
                     /gene="traI"
                     /locus_tag="OO515_000628"
     CDS             4948..5766
                     /gene="traI"
                     /locus_tag="OO515_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HCR6574643.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRRNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            5763..6911
                     /gene="traJ"
                     /locus_tag="OO515_000629"
     CDS             5763..6911
                     /gene="traJ"
                     /locus_tag="OO515_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCR6574644.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVVPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAVSITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            6908..7198
                     /locus_tag="OO515_000630"
     CDS             6908..7198
                     /locus_tag="OO515_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6574645.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            7213..7764
                     /locus_tag="OO515_000631"
     CDS             7213..7764
                     /locus_tag="OO515_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HCR6574646.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            7854..11621
                     /locus_tag="OO515_000632"
     CDS             7854..11621
                     /locus_tag="OO515_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6574647.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESLASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATTASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTDTVE
                     PAVRDDPTPQQPVQSTSSSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PAPDSIVYAPERPDAPISLNLDEIIKSLEGEERADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATDFYGGVIELTGSDAFKQKAMQLIIEHNIKVRMKFPDQRAA
                     LEKLRKEMAVGKDTVVTHKPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            11639..11986
                     /locus_tag="OO515_000633"
     CDS             11639..11986
                     /locus_tag="OO515_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6574648.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            11983..12675
                     /locus_tag="OO515_000634"
     CDS             11983..12675
                     /locus_tag="OO515_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCR6574649.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            12686..13669
                     /gene="traN"
                     /locus_tag="OO515_000635"
     CDS             12686..13669
                     /gene="traN"
                     /locus_tag="OO515_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HCR6574650.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNT
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            13672..14961
                     /gene="traO"
                     /locus_tag="OO515_000636"
     CDS             13672..14961
                     /gene="traO"
                     /locus_tag="OO515_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCR6574651.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            14961..15665
                     /gene="traP"
                     /locus_tag="OO515_000637"
     CDS             14961..15665
                     /gene="traP"
                     /locus_tag="OO515_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HCR6574652.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAIVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            15665..16192
                     /gene="traQ"
                     /locus_tag="OO515_000638"
     CDS             15665..16192
                     /gene="traQ"
                     /locus_tag="OO515_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCR6574653.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            16243..16647
                     /gene="traR"
                     /locus_tag="OO515_000639"
     CDS             16243..16647
                     /gene="traR"
                     /locus_tag="OO515_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HCR6574654.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLASC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            16711..16899
                     /gene="traS"
                     /locus_tag="OO515_000640"
     CDS             16711..16899
                     /gene="traS"
                     /locus_tag="OO515_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HCR6574655.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            16883..17683
                     /gene="traT"
                     /locus_tag="OO515_000641"
     CDS             16883..17683
                     /gene="traT"
                     /locus_tag="OO515_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HCR6574656.1"
                     /translation="MQHSIKNLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLVKLIRRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTEPLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            17773..20817
                     /gene="traU"
                     /locus_tag="OO515_000642"
     CDS             17773..20817
                     /gene="traU"
                     /locus_tag="OO515_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCR6574657.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWALSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            20817..21431
                     /gene="traV"
                     /locus_tag="OO515_000643"
     CDS             20817..21431
                     /gene="traV"
                     /locus_tag="OO515_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HCR6574658.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            21398..22600
                     /gene="traW"
                     /locus_tag="OO515_000644"
     CDS             21398..22600
                     /gene="traW"
                     /locus_tag="OO515_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HCR6574659.1"
                     /translation="MQRKTLLAALIAILNGTAYQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            22629..23213
                     /gene="traX"
                     /locus_tag="OO515_000645"
     CDS             22629..23213
                     /gene="traX"
                     /locus_tag="OO515_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HCR6574660.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRRIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            23310..25478
                     /locus_tag="OO515_000646"
     CDS             23310..25478
                     /locus_tag="OO515_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCR6574661.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSDPTLIGSMFWVFNNIIAVLAIVWCIFISIRHVVRSGHQGQVFSV
                     GRDIVGILSFVAGFLMIVPTGNGWSLAQLIMLWGASIMGVGSANVMVQVAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGTASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQKAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYK
                     TQLQNTTYTPPLGTVTSANEQRMANAQDPKSALSEMMSFFVYRTNWIATEISGTGTTS
                     DQVNPLIKMKNIGDYTLGTTEILWSGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVA
                     LLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGREGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKNNTNGSDDKDGIKG"
     gene            25543..26205
                     /gene="excA"
                     /locus_tag="OO515_000647"
     CDS             25543..26205
                     /gene="excA"
                     /locus_tag="OO515_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCR6574662.1"
                     /translation="MKAKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGVPVAE
                     VF"
     gene            complement(26277..26486)
                     /locus_tag="OO515_000648"
     CDS             complement(26277..26486)
                     /locus_tag="OO515_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR6574663.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDITLAAIYALGEGRCQADNIIRE
                     LERLSQSDDMEIKIAAIKALGRIYR"
     gene            26818..26994
                     /locus_tag="OO515_000649"
     CDS             26818..26994
                     /locus_tag="OO515_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574664.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            27653..27904
                     /locus_tag="OO515_000650"
     CDS             27653..27904
                     /locus_tag="OO515_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574665.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(27976..28128)
                     /locus_tag="OO515_000651"
     CDS             complement(27976..28128)
                     /locus_tag="OO515_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR6574666.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            28401..29387
                     /locus_tag="OO515_000652"
     CDS             28401..29387
                     /locus_tag="OO515_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574667.1"
                     /translation="MRSRYVDRIIYMKKLLIRLIPDAIYEALEKTALHSERSLEAQAR
                     YILSCSVDNEKQLTGGERYQREITARLNQALSEANEVITAINLVPARIAEQLGHHDAI
                     ESENWFTGNAVPSFTELDELSDIFGCSPDWLKFGENVPYPKSSKGRINWNRGGEKDID
                     ALLEPDNKGRKVSSIHIFRVNESGNILILREFENSITTDFFSTNLYLSDKEKIGQGGF
                     HDLVDFLVILQSLYLKYINSNLLVKSYNISQYVLDSCYKSGEKHPITICSAGETSTWW
                     EDIWHEDMIAQSRNNESYFWDGDKPLIDSLHKELQKNNRLKPNSELDMPLIG"
     gene            29605..30813
                     /gene="trbA"
                     /locus_tag="OO515_000653"
     CDS             29605..30813
                     /gene="trbA"
                     /locus_tag="OO515_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCR6574668.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYTSCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPKKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            30832..31902
                     /gene="trbB"
                     /locus_tag="OO515_000654"
     CDS             30832..31902
                     /gene="trbB"
                     /locus_tag="OO515_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HCR6574669.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            31895..34186
                     /locus_tag="OO515_000655"
     CDS             31895..34186
                     /locus_tag="OO515_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCR6574670.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     LTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(34223..36403)
                     /gene="nikB"
                     /locus_tag="OO515_000656"
                     /pseudo
     CDS             complement(34223..36403)
                     /gene="nikB"
                     /locus_tag="OO515_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
     gene            36412..36714
                     /locus_tag="OO515_000657"
     CDS             36412..36714
                     /locus_tag="OO515_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCR6574671.1"
                     /translation="MQLAKAALSELPDLTAQRKTLRDFLDAIRDDIIVLVRTKGYTLA
                     DVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGRKDGIDMNNN"
     gene            complement(36800..37648)
                     /locus_tag="OO515_000658"
     CDS             complement(36800..37648)
                     /locus_tag="OO515_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6574672.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNNQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPSRV"
     gene            37997..38194
                     /locus_tag="OO515_000659"
     CDS             37997..38194
                     /locus_tag="OO515_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574673.1"
                     /translation="MQGAPHPARPEKTDGTTTACGGHWYTTPAQVKTEKTARHPQSKK
                     VKFLHFFTLIFRADLVIMVIK"
     gene            38228..38479
                     /locus_tag="OO515_000660"
     CDS             38228..38479
                     /locus_tag="OO515_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6574674.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMHKADIIRRLENELGETAEDV"
     gene            complement(38510..38755)
                     /locus_tag="OO515_000661"
     CDS             complement(38510..38755)
                     /locus_tag="OO515_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574675.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(38755..39669)
                     /locus_tag="OO515_000662"
     CDS             complement(38755..39669)
                     /locus_tag="OO515_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6574676.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(39734..40000)
                     /locus_tag="OO515_000663"
     CDS             complement(39734..40000)
                     /locus_tag="OO515_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574677.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(40093..40527)
                     /locus_tag="OO515_000664"
     CDS             complement(40093..40527)
                     /locus_tag="OO515_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCR6574678.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(41256..41756)
                     /locus_tag="OO515_000665"
     CDS             complement(41256..41756)
                     /locus_tag="OO515_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR6574679.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(42219..42815)
                     /locus_tag="OO515_000666"
     CDS             complement(42219..42815)
                     /locus_tag="OO515_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574680.1"
                     /translation="MMKSDEQYHVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(42812..43531)
                     /locus_tag="OO515_000667"
     CDS             complement(42812..43531)
                     /locus_tag="OO515_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCR6574681.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(43528..43962)
                     /gene="psiB"
                     /locus_tag="OO515_000668"
     CDS             complement(43528..43962)
                     /gene="psiB"
                     /locus_tag="OO515_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCR6574682.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(44017..45975)
                     /locus_tag="OO515_000669"
     CDS             complement(44017..45975)
                     /locus_tag="OO515_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6574683.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWGWCAGRMEPVGECREDARAYRNLPEPEAVLTEAEE
                     EHLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADHTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGCGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            complement(46034..46267)
                     /locus_tag="OO515_000670"
     CDS             complement(46034..46267)
                     /locus_tag="OO515_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCR6574684.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            complement(46325..46810)
                     /gene="ssb"
                     /locus_tag="OO515_000671"
     CDS             complement(46325..46810)
                     /gene="ssb"
                     /locus_tag="OO515_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6574685.1"
                     /translation="MGNDPEVRYIPNGGAVANLQVATSESWRDKQTGEMREQTEWHRV
                     VLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTVQMLGRAP
                     QQNAQAQPKPQQNGQSQSADATKKGGAKTKGRGRKAAQPEPQPQPPEGEDYGFSDDIP
                     F"
     gene            complement(46960..48861)
                     /gene="ltrA"
                     /locus_tag="OO515_000672"
     CDS             complement(46960..48861)
                     /gene="ltrA"
                     /locus_tag="OO515_000672"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6574686.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDI
                     KGFFDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISP
                     LLANIYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESE
                     KEAIKKEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQV
                     RNFITTSLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTG
                     IVQLFVPDNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYS
                     LALDMKYKLSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRK
                     IEVFKLKHVNRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGRCEVHHVRK
                     LKDLKKGRKAGYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQP
                     PVSSGDSAK"
BASE COUNT        12852 a        13079 c        12190 g        11822 t
ORIGIN      
        1 attgttggca ttattttttc ctcttttgag gttagcatgc cgtctccacg catccgtaaa
       61 atgtccctgt cacgcgcact ggataagtac ctgaaaacag tttctgttca caagaaaggg
      121 catcaacagg agttttaccg gagcaatgtt atcaagcgat atcccattgc ccttcggaat
      181 atggacgaaa taacaaccgt tgatattgct acatacagag acgttcgttt agcagaaatc
      241 aacccccgaa cgggtaaagc cattacaggt aatactgtac gtcttgaact cgcccttctg
      301 tcatctctgt tcaatattgc tcgtgttgaa tggggaacct gtcgtactaa cccggttgaa
      361 ctggttcgca agccgaaagt atcctccgga cgagatcgtc ggctaacgtc ttcagaagaa
      421 cgtcgccttt ctcgctattt ccgcgaaaaa aatctgatgt tgtatgtcat tttccatctt
      481 gcccttgaaa cagccatgcg gcagggcgaa atactggcct tacgttggga acacattgat
      541 ttgcgccacg gtgtggctca tttacctgaa accaaaaacg gtcactcacg ggatgttcct
      601 ctgtccagac gtgcccgtaa ctttcttcag atgatgcccg ttaatctcca cggcaatgtt
      661 tttgattaca ccgcatccgg ctttaaaaat gcctggagaa tagccacaca acgacttcgc
      721 atcgaggacc tgcattttca cgatctacgg catgaagcaa taagccgctt cttcgaactg
      781 ggtagcctga atgtaatgga gattgctgca atatcaggac atcgttccat gaatatgctg
      841 aaacggtata ctcatcttcg tgcatggcaa ctggtcagta agcttgatgc ccgccggcgg
      901 cagacacaaa aagtggcagc atggtttgtg ccgtatcctg cccatatcac gaccatcgat
      961 gaagaaaatg ggcagaaagc gcatcgtatt gagatcggtg attttgataa ccttcacgtc
     1021 actgccacaa cgaaagagga agcagttcac cgcgccagtg aggttttgtt gcgtacactg
     1081 gccattgcag cacagaaagg cgaacgtgtc ccatctcccg gagcgttacc tgttaacgac
     1141 cctgactaca ttatgatttg ccctctgaac ccgggcagca ccccgctgta aacatcattc
     1201 aaaacacata cagaaaggcg agttgcaaca actcgccaaa cacattcgat ctgcatgatc
     1261 gaaattattc gtttcaccag aaaaaacacc gaagatacaa taaccacatg agctgaaaag
     1321 actttttcag tcggaggaac aatgagactg aatacgaccg gtattgcggc ccgaatgatg
     1381 ctctcgctgg acaaaaaagc aatcggcgaa aaactcatca acggtcgtca gataaacccg
     1441 acacctctgc aggtccgtta cccacaaggg gtaagagaag ttctggggat aatgagcgaa
     1501 caattagcca tatccaccgc tgatttaacc cgaatcctgc ttgaggatgc tcttcataat
     1561 atgtttatgc ctgcggataa caccacagga aacataataa gcagaattga atacatcatg
     1621 ctgtcgcatg atataaatgc gccattactg gcaacattgc tgtcgccatg gaatatccgc
     1681 tccacggtta tacaggaccc ggccagactt gcagactatc tttcagctga cgctctcgaa
     1741 catcttgctg aatgttttaa tctcaatccg gactggctga atggccatga aaactatccg
     1801 atagcgttgt ccggtgaatg gccagatact gctgataatt tcagaatgct tattaataat
     1861 agcagcaaca ctgaagttat tttctggcat agtttcccct tcgcaggaaa cacgaaaaga
     1921 gaatattgcg gggtaatatt gagacaggaa aaagaaataa atggctctgt aatatatccg
     1981 gcactatcat tatcaccaac acttctgaac gatgaaaaaa gaaaatggtt aactgaatat
     2041 acaaccaggc agaatacaac aatgtctttg cgacgcgtca cgttgagacc tggccttgcc
     2101 gagaacctta ttacagggca aatactacct gttagcttat ttaatacgtc tttattaccc
     2161 tggtaaatac cagatagtac caaagtaaaa aattacggca catccgtgtc atagcgttgt
     2221 tttgtctaaa ttctgataaa caggaaatta aatgatgaag aaaaatcata ttacccgcac
     2281 catcattgca tctgctgtat tattttcctt taacgctgca gcagcgacgt catattttga
     2341 agcgcgtaac gatgcaatgg gtggcaccgg ggtagcatct tcccattatg gtgtggcacc
     2401 tctggcaaac ccggctttac tgacaaagca caacagcaat gatgatttca gccttcttct
     2461 gccgtctgtt ggggcacaag ttgctgatcc taatgatgta tcaaataaag ctgatgatgt
     2521 taaagatgac tgggatttgt ttgacagtgc tgtagataac cagcatggcg tacaacaggc
     2581 agcggcaaat cttaaacatc gtctgcagga attccgcaat atcaatgcag atgctcaggt
     2641 aggtgtatca gctgtagcag ccatggcaaa tgacactctg ccgtttgccc ttatggtcaa
     2701 gtcttacgga actgtcagcg taaacggaaa agtaaacgat gcagacctgg attatctgga
     2761 caaagttgcc aatggaacaa ttaccgacgt cgataagaat gccctgacct cacgcgcatt
     2821 cgggcgtgca gctgtaatta ccgacgtcgg aatttcattt gccaaagaac tggaaacggc
     2881 cggacaaaaa tggtctcttg gcgtcacacc aaaatatcag cgtgtggacc tgtttaacta
     2941 caacgtaacg gttcgtgatt acgacaaaga cgattttgac ggtgacaagt accacaacac
     3001 gaaaaacgga ttcaacgccg atattggtgc ttatacagac ctgaatgaca actggactgt
     3061 cggtcttgtt gcccagaaca tcattccacg cagtattgat actaaagtcg tcaatggctt
     3121 taaagaaacc ttcaaagttc gtccacaggc aaccgccggt gtatcctggc ataatgacct
     3181 gtttaccact gctcttgacg ttgatctgac tcctgccagt ggcttcactt ctgacagcaa
     3241 acgtcagttt gcaagtgttg gtgctgaatt taacgcatgg aaatgggcgc aattgcgtgc
     3301 cggttatcgt cagaacatgg catcaaactc cggaagcgcc ttcaccgctg gcgttggtat
     3361 ctctccattt gacgtcgtac atatcgatgt ttccggcctg gtaggaactg accacgatta
     3421 cggtgcaatg gcacaacttc agttcacttt ctgattactg tccccgcccc cggcactaaa
     3481 cccgggggta tttttatatt cattttaaat aacatgatga aaccacgttc ttcttattca
     3541 aagacagcat tcattctttt attttctgtt ttcctggtag ctgcagtgac aaaagcaaaa
     3601 agctcgcttc ccgatatcac cctagaacag gcaaaagaaa ttaatgctga taacaccgtt
     3661 attttccttt tccgccatgg agaacgttgc gaccgttcag atatgccatg ctattcggat
     3721 aaaagcggaa tcactattac cggtacagag aaagcacagc aggaaggtat taagttcgca
     3781 acaatcttca gtgaatatga tatttactcc agcaatgcgg taagaaccat acaaactgca
     3841 aaattcttct ccggtaaaga ccccgtcgtt atggacagtt tatcagactg caataatgat
     3901 ctttataaga cgcttgaatc aatcgcaaga gaatctcata aaaggaacat agtaataatg
     3961 acacataatc attgtttatc atttctggcc agagataggc tcggcaagaa attcaaaccg
     4021 gcgtaccttg atgccctgat catgcattac gatggtaccc ggctcatact tgacggaaaa
     4081 tacaacaaag aggcatagag gatatttgaa tttacataaa ttctgatata ctcagtaaga
     4141 tacaagagtg tacgggcaca tgtgcctgtc tgaattgcag gtcatccgga cgagggccgg
     4201 gaactgtttc agggacaagc gccggtacgt tcgattcata aatcgtcgag tcatccatca
     4261 tggatcgatt cggcgcggag cgttaaagat tacgctcata ttcgttttat gtctgacgac
     4321 acatcaacca gccagtatca ggaataatca ccttcagtaa actatgaggc tatttacctg
     4381 gccacactgg catttatttc cggtaacgca agtatagccg gaaacaaaca tcatcctgtc
     4441 tgtaccggag caccggtata tcccattctg atttaactgc tcaggaatta ttatgaaccg
     4501 tattatacct gcggggtgtc ttgccgccct ttttctggct ggctgcaact ccccttctca
     4561 gcatactcct gttccgcgta actacggtgc tgcctctgat gtggcggtta cccggcacac
     4621 tcagtacaca ttatgggaca aaggtgtgtt ttccggccaa cttccggttt caacggggtc
     4681 tcatcttctg acagctaaca gcgatcgcct ttcctttgac tgggaaggtg atgcaattga
     4741 attactgaac gaactggcca gagtcagggg gatgcagttt aactacaatg gtgtccgcct
     4801 gccgttacca gtcaaccttc acgtccggga tatgacgttt tccaatacgt tgagacttat
     4861 tgaagcccag acagcctgga gagcaacaat ccatcaatat ccgggcttgt tacaggtcag
     4921 cttcatgcag ccggagaacc ggaaaaaatg aaagcactca cctctctcat tttgccagta
     4981 atactgctta ctggttgttc ttcttcccct accatctcgc caggtggtgt caatacgcca
     5041 ccacctgaca ttaatgcatg gcttaaacca cgaagtgaaa agccggatag tctgtcagaa
     5101 acacgctgga aaatgctgac cgacgcaggt aaaacgcttg gatttcgtgg tggcaaggct
     5161 cagcgttcat gggagctgat tcaggcactc aacgcccggg aaagcacact taatgcctta
     5221 tacgatttcc ggccactgat tagcccggaa ggctggctac caccggttat tgatgaagca
     5281 caggacgtag ctcatatcac gccagatcaa atccggacct cttcccgggt ctggaccatc
     5341 attcgccctg aacgctttgt cagcaatcca ccgggctggc gtgactggct tctccggggg
     5401 ctttcaacaa cagccactcc gggtacagaa ggcagcgtcg ttccagaaga cagcgtgcaa
     5461 cgaaaagtgt gggaaaccgc attacggcag ggatggcagg aaggaagacg aaacgccgac
     5521 ctgacgcttg aagccaacca gaaaaccctc acccgtgatt accggggcat gatgctgtac
     5581 tcacttctct ggcgtcaggg catgatcacc cgtccggatg tttctgatca aatgcaaacc
     5641 gttacaggtg acgggaaaaa actggtcact ggagatcgtg ttcgtcgcct taaaaatcat
     5701 gcggaattca atcttcagaa atctcactgg cgtccgctta taggcactga aggaggctcc
     5761 cgatgaatat tcctgatgag tttggtcttt ttccgtttca gcgtttcact gcagatgaat
     5821 tacggcattt ctttgtctgg tgtgccgcac ataaagtcag tgatgtggat ctgaccggtg
     5881 ggtcacctgt atctgtcagt cgctttggcc gacgggtgcg ttgttccagt gcaacgttac
     5941 caaccacact catgtcctcc ctgattgatg aactgttcgg tcgggaagtc gttccccgcg
     6001 ttctggcagg caatccggtt gacaggacga tacagattaa cggggatgca tccggtcgct
     6061 atggtcttaa acgcggagaa cgcatccggt tacgctccca cctcattcag ggcacatctg
     6121 gtgcagagga gaaagcagtc tcaataacca tgcgtgtcat cccgacagaa ataccggaca
     6181 ttctctccat gaacattgaa ccagacctgc ttgaagcaat ggtctgcaaa agcggtctgg
     6241 gatttgtatg tggtgaaacc gggtcaggaa aatcaacgct gtgttcagca ctgtaccgtt
     6301 acattatgga caatttcccc gacgccaaaa tcgtgacata tgaagacccg gtggaatata
     6361 tcctgggaaa tgaaaacgat ctgctcccac cgcatcaggc agaaatcggg cgtgatgtgg
     6421 tcagttttgc tgccggactg cgttctgctg tacgacgtaa tcccgaaatt atcggtgttg
     6481 gtgagatcag ggataacgaa accgccgacg cagcggtaca ggcaggcaac accgggcatt
     6541 actgcctgag caccatgcat acgaaatctc ctggtgaaac gctggcacgt cttcttgggc
     6601 tgtttccccc tgtcatcagg gattcgatgg cctgggctgt cctgtcactg ttgcagttca
     6661 ttctggttca ggttctggtc agaacaaatg acggtggtcg taaagccgtc cgggaatata
     6721 tcgtcattaa cgatgagctt cgcgacaatc tttccggaat gccgcatgca gagtgggggc
     6781 atcacattga tgccataatc cggcaggaga agcgccgtat ccgggaccag attctggaaa
     6841 tgtatatccg gaatgaagtt gaccgaagag aagcgattct ttttatcccg ccaggggagt
     6901 tacggtcatg aaaagagaag gtttctggcg atatgcaaca ccgccagtca acatccaggg
     6961 cgtacctctg ccgttcacca tgatttatct gtttatatgc ccctttccct ccaaaacaac
     7021 cttctggatt tgcaccggca tcattttatt tttcgtcatt ctcgaccgtt caggctggac
     7081 tgtcaggacg atctgtactc gcattttcag cattctgaga ggggccatcg cctccggtcg
     7141 tccatggtgg tatcgccacc atacggaatc accacgagac tggactggcc tcgattaaat
     7201 tctggagtct ttatgtcagg taaaaaaact atcgttactc tgctccgggt atcgctactg
     7261 gcatgcccgt tactgttcac gacaccatcg tttgcaatga tcgacacgcc atccgtgaag
     7321 gtcggctttt ctcctgaagg aagcgcatcc gctctggtgc ttgacacaat caacagtgcg
     7381 gaaagctcca tcaggatgat ggcatattcc ttcaccgacc cggatgtaat gcatgcgctg
     7441 gcaaaagcga aaaaacgcgg agtggacgtc cgtattgttg ttgatgacaa ggggaatacg
     7501 aaccgggcaa gtcaggaagc gatgaaatat atcaacctgc tggacattcc tctccggact
     7561 gtagatgcct tccccatcca tcacgacaaa gtcatcattg ttgacggaaa cacggttgaa
     7621 actggctcct ataacttctc tcgtgctgct gcacgcaaga attcagagaa tgtcgttgtg
     7681 ctcaagaata tgccggatgt agccgcacaa tatcttgaac actggcagga tcgctggaat
     7741 aagggtacag actggagacc ctgacaaaca tttctccccc gattttctat ccccccagcc
     7801 acccccggct tccggcggtg gtttttttcc gtcttttttc aggagaagca ctcatgccgt
     7861 ctgtatcccg ctaccgcacc tggcttgccg tgcctgcaga tgaaatagaa gatctcaaaa
     7921 aagcacatcc cccgatgaac gggcacacgc ccgtcatctg ggacaaggag cacaaactct
     7981 ggttcgcccg cccgggggcg gacctgtcca ggctggaccg ctggctgccc cggccacagg
     8041 acgtctccat gaacggcagt gatccggtca ccgagtttgc ccaggttctg gagaatgccg
     8101 gactggttct gaaagaactg ccggtcatgg acgggaaaat ccaccgtgtc ccgaccgccg
     8161 atgacaaaaa aggacaaaaa agtggcgcat atcgcggatt cctggacggc agacctgccg
     8221 gctggtacag ggattatcgc agcgcggata attcgccgat cacctggacc ttctccggcg
     8281 gagaacagac agatccccgc gcaagactcc acctgaaagc gcattcaatg caacgcaggg
     8341 aggacgcgga gcgggaactg aaagcacagt acaaccgaca ggccgcgtat gcccgccgct
     8401 atatcaacaa atggccgcag gccacagcac atgaatacct gacccggaaa ggcattcagg
     8461 ctgcaccggg ggtgcgggta aacaacaaaa atgaactggt tattcccttc agtaaccgta
     8521 acggcgcgat acgctcttac cagcgtattc cggtcaccgg ggggaaagat gcccgcatcc
     8581 tgatagattc ggagaaaacc ggtaactggt ttgcgctggg cacaccgcgc aacggtcagc
     8641 cggtactgtt tgccgagggc tatgccactg cggcctccct gcacgaagcc acgggccttc
     8701 cggtactgat gacggtggat ggaggcaaca tgattgccgt tgccgaaaat gcccggcaaa
     8761 agtggacgca aagccccttt atcttctgtg ccgacaacga tcatgcaatc cgggttaaca
     8821 aaggtatcgt cagtgcaaca aaagccgcag aactgacagg aggatcggtc atctttcctg
     8881 cgttcaccga tgccgaaaaa gcgcaggggc tgaccgattt caatgacctg gacgcaagcc
     8941 ggggacgggc cgcttttcag catgtcatca acgcccagtt agaacacatc ggggtttcca
     9001 cccccaacag taacaccccg gaaatgcgcg aggcactggt gataggcaac ctcgttttta
     9061 caccggtcca cactgaggaa aaaaccatga ctccgacaga atatcctgaa acatcacccg
     9121 atacagggca cagccatgac cagggacctt ctctgccgtc cgccacgcag caggaacaac
     9181 cagctgcttc cagcagtaac attgctgatg aaacacagtc atttaccagt cacgccactg
     9241 aaaataacgg caaagatgag cggcacgctg ataacgttca ggcgcaaacg gaaagctctg
     9301 tagttcagga ctcccctgca gaatcactgg cgtccccgga aagtacaacg gcgtctgccc
     9361 ctgatgaacc cgctcatccg gcggaacctc cggaaaaagt tgtctcggtg gccacagata
     9421 aagactggcg tgaatttgaa gctgaactgt cacaaccgga aaaaaacgag tcacaacagg
     9481 aatccggcac ttcacctgaa ataccggccc ctgctgccac caccgcatcc cctgaagaca
     9541 gtccctcatc tgaaaccatg aatgaatcag ccgagccatc accttcccct gatgcagtca
     9601 aagaggaaat gaccgtgatg acggagcctg atgacaaaca aagcaccgga atatccccgg
     9661 atactccccg ggaagaggcg gtcaaccatg taactgaaga cattcccctt cagccagagc
     9721 cgttccttcc tgagtcagat ggccccgaag aatacgaaaa ctacagcgca tatcaggagc
     9781 tgatgaacaa tgaaaatcct gagcatctgc aggaaaacag tgatatgccg tcgcaaccgg
     9841 acacaggcca cgttcaggct gatgagtcag aacttcaggc cacaacagat acagtagaac
     9901 cggctgtccg ggatgacccc actccacagc aacctgttca gagcacatca tcttctgata
     9961 atacttcatc gtttctcgac aaagcgcgtg gcttttttac gcgtaaaaaa acggattcac
    10021 aggcgcatga gaacagtgat cccacaccct caccggaaac cacgacgacc acaccggcac
    10081 ctgacagtat tgtctacgcc ccggaacgcc ctgatgcacc gatatcgctc aatctggacg
    10141 aaatcattaa atcgctggaa ggtgaagaac gagcagacag aaccgtcctg tacaaactgg
    10201 atggcaaacc tgccttcatt gaccgggtaa accggctgga aatggtcaac ggtgccagca
    10261 atgacgatcg cagtgttctg gctgcacttg ctgtggccac tgatttttat ggtggtgtca
    10321 ttgagctgac gggcagtgat gcctttaagc aaaaagcgat gcagttgatc atcgagcaca
    10381 acatcaaagt gcgcatgaaa ttcccggatc agcgagcggc gctcgaaaaa ttacgcaagg
    10441 aaatggctgt cggtaaggat actgttgtca cccataagcc aacaccggaa cttaaccgga
    10501 atacccctga acaaccagca gtcccagacc ctgttcagga aaaagaagcc acacaatccc
    10561 cggcgtcccc agtagcccct gaagcatcaa ccgtcagcac cgtccctgca tcatcaccgg
    10621 ctgaacctgg caaaacggca gatgcggcac aaggtgaaga accaccagga aaacttcgcc
    10681 cgggagagag cgtgactgcc gtactgcaca attttggtcg tgctgaatat gcacccggca
    10741 aaggagagag tttctttgtt gagcttaaaa accgcagtgg cagcaaactc tactgggggg
    10801 aacaactgga aagtctggtc aaaaaccatc agaaaggtga cgtggtgaca ctgacacttc
    10861 agaaccgcga acaattcatc ctcccgggag agcaaaaagc acgtttccgg aataagtggt
    10921 ccatggagtc agttaccaac gggatctcgg tatcacatga taatccggat aaaggtcagc
    10981 gtattcaggc gattccggtc gaaaccttta tgaaagtggc tgcgcaaatc agccaggggt
    11041 ggcccgaaga gatgaaagcg ttacgtatgc cagaaaacgt cggctcacat ctgtttatcg
    11101 gtgaagaccg acatccggtc tccgcgccac agaacgcaaa tcaggtaaca gaaataacca
    11161 gcgcagcccc tgacaagctg acacctgttc tgggatcagt ggataaggat acgcgtgaac
    11221 tcaacctgct acttgtccag agtgccgatg aacatctgca gggtgtggtc cggctgaacg
    11281 gcacattgta tccggcactg gccaccccat cagccgacaa cagtcagctg gttatcaatg
    11341 cgctgaccga caaaggttta cgctttgcag gttatggtga agcggtaaac cacgatgctg
    11401 acagcacgaa tcgccctgca ccggagctta tgcagtttca tctgaaaacc cgggaagaac
    11461 cgctttttgc tgctgtctac acgccggaaa aacaacctga tgcgttgtac cggaacctgg
    11521 gcttcgagca gtcatggcag cagtggtcca acagccaaaa gccggaggac aggcaggaaa
    11581 aaactctgca tcaggatctc tcccacagcc ccggccgatg agccggtaag gaagccatat
    11641 gaacatgcat aagtctctgg ttgccgtgct cggccttatc ctgatttcat ccacggcaag
    11701 tgctgcaacc tgtcgtgcgg gtagcgcggc acaggccggt agtaatacag gatatgagcg
    11761 agcacgtgca gctgcggatg catggtcaca acgtgaaaac gatgtttcct catccctgca
    11821 gtcctgtctg tcacgaatca aaaaaatcag cataaacctc ccccagttcc ccagccttga
    11881 cgacattctg tcgcagctgg aaaaccaggt ctgcgacgcg gtcgtggaca aggtgaatga
    11941 aaagctgccg gggaatattg atccctggaa ggactacaac ctatgaccga aaataccgag
    12001 ccatcctcgc catcacatcc ggcacctgac ccggaaatac tgaagagcac aatcagggct
    12061 atgaagcagt cagaacagcg ggcaaataat gtccctgcgc tgataaaagc gcttttatgt
    12121 acgggcacat gtcttcttat cagtattact ggtaacgcca ttcagtactg gcactcaacg
    12181 aatgtggagc gcgaatattt cgctacagac aatggtcgtc tggtcaggct ggcaccaaca
    12241 agtcagccag catggagtca gaatgatgca atggcctttg gcagccaggc actggcgacc
    12301 gcattcaact tggattttgt gcactatcgt tcacagatct cctccctgtc tccgcgtttt
    12361 tctgacgaag gttttgtcgg atatgtgaat gcattacagg catctaacat tctggagacg
    12421 atcaagaaag agaagatgaa tctgaccgcc acgacaggtg ctggcgttct ggtacgacag
    12481 ggacaaatgt ccgacggcgt atggttctgg acattccagt atcctgtccg tatgcgtctg
    12541 gtcggccaga caaccagcaa gccagaacag tcttttgttt tcgaaatcac catccagcgt
    12601 gttgacccca gactgaaacc atccggtatg gaaatccggc agatgatctc ccgtaacgcc
    12661 ggaccgaact cctgagataa gtaatatgca atcacgttat ttactgagca cactgctcct
    12721 ggtgtgcagc gccgccacgt ctgcagataa cgcaggatgg cagaacgcac gcacaccaca
    12781 aacgacaaat tctgccagcg atcacgttca gaacacctcc caaaacaccg gaggtgttcc
    12841 ggcaacactg gtaaaaggtg agctgcctgc ccccgggcag gccagcccgc tggtacagga
    12901 tgcggcaagg cttgattctg agctctccgc cgatgagatt cgctcactac gcagtctcat
    12961 ggcggataac gagcgggcca tcaatgcgcc catcaccagt gttgtacccc gtatcagctc
    13021 tctgacagtt aatctgtccc ccggtgccag ccttcctctt gttcgcacag ccatgaacaa
    13081 cctgagcgtg gtgacattta ccgatatcaa cggttcacca tggccacaat ccgatcctcc
    13141 ttataacgcg gcaccaaaac tctttgatgt gcagtacaac gagaacatgg tgacaatcac
    13201 accattgcgg ccatgggcat ccggaaatat ttctgtctac ctgaaaggtc tgtcggtacc
    13261 ggtcatcctg aacgtaacca gtggcgaaac agatacgccg tcatccagtc aggaaatgga
    13321 cagtcgtctg gatctgcgaa taccacggca gggaccaacc agcccagttg tctccatccc
    13381 gacggataag atcgccctgc atgatgcaac attacaggca tttctggatg gtattcctcc
    13441 ccgggaccct tcagttaaga ggctgaaatt cacaggtaat gtcccggata caacgatctg
    13501 gcaacacggc gatgaccttc tggttcgttc ccgggcgatg ttgcgcgatg aatttgaaca
    13561 gaccttgtcc tctgccgacg gaactcatct ctggaaactt cccgtcaccc cactactgac
    13621 tttctctgtg aatggccagt ccgttcacgt gacaccagaa ctggagtaat catgagtgct
    13681 gaacaggatg ccggtaaatc cggaaagaaa ctggctgccc tgcttggact gggcagcatc
    13741 atcttatttg gcggcggcta tatcgcgttt tccaaactat cagggagtaa cagcgatatg
    13801 cagtcagccg tgaacattaa cagtgcagca agcggcggaa cgcgcagcgt taccgaaacg
    13861 cctcactatc gtgaactgtt aagagcggat aatgaacgag gggccgcagc tgcagcccgc
    13921 aataatcaga cgttcattgc cagtcttcca caggggcttg atattccgga cactccggaa
    13981 aagcaacaac agcctgcagc aaaaccggaa aactatgctc atcgccaggc ttccggcaca
    14041 ccacaggagg atcgagcagc cagtgaaaaa cgtatggagc ggctgcaaaa gctgattgta
    14101 agaataaaag atcagcatcc tgccggtagt acaccaacca tagcaacgac tatgtggaat
    14161 aaatctccgg cagaaacaac agggcagaat ggtacgcagc aattcgcatt acagaatgcc
    14221 agcctgtcca cgcctgtagc agaaaaaggg atacaactga tacctgctct gacccgtata
    14281 ccggcatata ttgatactgc agtggattct gataacccgt catccaaggt tatcgcaacc
    14341 attcccgctg gcccctgggc aggggcaacg ctgttcagtc cgggagtcaa actggtgggt
    14401 aacggcgtgg agatccattt cgaccggatg agctggaacg gcatggacct taaggtcaat
    14461 gcatatgctc aacgggaaga caacctgatg tcatccgtgg ccagcaatgt gaatacacgc
    14521 tggtttaaac atatcattct gccgtcagtg ttaggaggtg tgggcagtat tggcacactt
    14581 tataaagatg ccaacacgca ggttattcag ggtaactatg gcacggttac cggacgcgtg
    14641 ggaatgccca gtggtgaagc tgttgcgggc gttattgccg gtggtatggc ggaaagagga
    14701 agtcagatat taacccgcca ggcagaaagt gagccctata aacaggtaga agtgtatcag
    14761 catgaagtcg tatcaattct gtttgttgac ccggtaatga caaacgacgc caggtcatct
    14821 tccctttcca gtggcatttc cccttcagtt aatcgtacat cacaggcgga acaacgctcg
    14881 caggcacgaa tgcagacagc catggagcaa agaaaagctg tgatgcaacg tcggtatgat
    14941 gaacaaccgg agacaccata atgaaacctg aaacagaaat agatgaaacc ggcagcttcg
    15001 gtgaacccga agagaaaccc gcccccttct ggaaacgttc cgtctggggg atatctgtag
    15061 caacatgggg gctatgtgca atcgtgttgc tcgcagccat atggtatctg ttcctgagag
    15121 cgccatcgga aacaggcatg cctccgttta atgatgcgga tgctggcgta cagacctggc
    15181 aaaccacaca agaatcatca ccttccgttc agtccactga gactatgact gtccggacag
    15241 gagaagatat gagccagctt gcccgggatg taaaaacaga gctggataac cgcgatgaaa
    15301 aaattcaggc aacactgaat atgcttcacg acagcattaa caagctgggc gaagctataa
    15361 aaaaggatga agaatacgca caggaaacac gccgtcagct ggatgatatc cggagccgcc
    15421 tgaacggaat aatgacacag aaaagcgtca ctgaatcctc ttccacaccg catccagcaa
    15481 agaaaaaaac gtcgtctgta cttaacggaa tgaaaatcat gtcgatggaa actggtatgg
    15541 cctggattcg ctggcagggt agtacctggg ctgtcagaga aggtcagacg ctgggcaatg
    15601 tggtgatcca gcggattgat ccgactaccc ggaccatcat caccagtgca gggacgttac
    15661 gctgatgaac atggatgccc tgaccgccat tgagaacttt gccagcagta ttttcagtgc
    15721 cggaatggat ttcctgttta cctggggaga attcatcggg gtgatcagca tgattaccct
    15781 gtttgcccgc gcccgttcag ccggtccggt aaaaatgtct cccggaaaat ttattgcggg
    15841 gatgttaacc agctgcatgc tcgtatcact cccggcaatg attaacgccg gaggcgtaca
    15901 aatgggattc agggctgact catttggccc gattgcatat gtacaacctc agacgtttgg
    15961 cgctgctgct ggcgcagcaa atgcagttct gtcgctggcc aagctggccg gtgttggatt
    16021 tgtaatgaac gggatatcca tctggcgcaa ggccggactc gatggacata ccgcactgag
    16081 tgccagtgaa tccgtcagta agggaaacgt caaatttatt gccggcgtcc tgctggtctt
    16141 tatcgatcgt gtcctcaatg ccctgctggc aagcattgga atcgtcttct gatttttttc
    16201 accttcatat aaaccactga tatatccatt taaggagaaa ctatgtcacg tattcgtgtc
    16261 gtagcggccc gtctgtacgc cgcatttatc cacgcacaac tgtttgtaat gtcatgccgg
    16321 agcaaactga ctaagttcct gctgctcatt cccatgatgt tgctgcccaa agccgttctt
    16381 gccgatggag atcttgccga tatggttcgc aatgtcgaac aaggtgccaa aacagcacag
    16441 tcatcatcac tgactattgc gcagttcatc ggcgtaattc tgtttctcgg gggcctgatt
    16501 ggcctgaaaa aagttgggaa acaagggggg atgggactgg ctagttgcat tgtatctatc
    16561 gtcattgggg cggtacttgt cgccggacct gaaatgatga gtcgcagcca gaaacagttg
    16621 gggatttcct caatttccat tggctaatct tgtccagggc actggcattt atccggtgcc
    16681 cttcctgttc ggcaatacaa ggaggcacat atgtctgcct ggagcccgga gcacataacg
    16741 ttacgacgaa cagaaaacgg caatgtgatt gcccacgaca gtcgggacga tcaggaattc
    16801 atttttgcag aatgctgttc cgatgaaatc gcaaacatca ttctggaagc cgtgaaacgt
    16861 tacaccagta cggagcctgc tcatgcaaca cagcattaaa aacttgtggt tgtatccctt
    16921 tccagaaatt gatgtggttc atacacagga gccactgtta ccggaaccgg aattaacaac
    16981 gcccggtcga tgtatctgct gccggcaaaa cgtcaggcac cgtttcagac tggatgactc
    17041 atggccactc agacagctta ccgacacaat cagtgacaca cgagttcgtc ttaataaggc
    17101 cacagaacat ctggttaagt taatcaggcg tggtgaacca gtcgcaaccg gcgaaaagga
    17161 aaagtacaac acggcagtaa aagccgctga acgggcgctt gagcaggccc gtctgtctgc
    17221 cagacgtctt tcactccgcc atgtacagaa agctgaaatc accagtacag agccgttgtc
    17281 agagaaagag caggaactgt ttcatgaaga cggcccgcct tacagcttat gtgctttctg
    17341 ccacgcctgg cattcactga atggttatgc cgcagcacag ggcgttatgg tgtggctacc
    17401 cgaccttcat ccatccacag tcgtggccct taaccgccgt tcattacagg aagttttcag
    17461 taatgataag ttccgggtcc ggcggggacg ggaagcgctc tctgcactga tgcaaaacag
    17521 actggcggtt gaggataaat tccgcagttt ccgcccggcc gatttcgctg acgtttttcg
    17581 tcgctatcca ccttccggtc gttcgcccct gcgtgaaaaa atgaacggga ttgccctgat
    17641 tctgacacca gattcattca tcaaaaaaga atacgttgat tgatttttct tcatgccact
    17701 ccagcgcggg gtggcattaa aaaaatcaaa ctgcacaaac ctccctcctg taagtccgga
    17761 gttccctcac ctatgaacat aaaccgttta atcttcacta tcgaagactg tctgaacacg
    17821 ctatcccgtt tctctgtcgc aagcagtttt gttgaatatt gcgatttacg tactgtaatt
    17881 ggtctggaca gacaggatcg tgaacgccgc ccatggctga attcgcccta tattgcagcc
    17941 acaaagcgtg gagaatatct ctctgtcttt gaagtcagcg gtgctttcag ggaaatggat
    18001 gaagcatccg accagactgg cccaggttca ctggaatcgc tgatcaccag catgagtgac
    18061 agcctgaata cggcatataa aaacagcggt cataaaatca gcttcgtttt tgagcgtgat
    18121 cctgacatgg ggaaagaaga aatcgaagac atggtcgccc cacaaaagcg ctcactggct
    18181 aatacaggta tccagttaca ggatgtggtc gatgagaaag tcacaacact gtccccatgg
    18241 ctggtccggg agcgttgctg gctggcgatc tggagtggac ccgaccttat cagcaacagc
    18301 gatcgcaccg cccatgatga gcttgtcaga agactggctg aacgagtacc gaaagcacgc
    18361 tttgcccagt ccccctggca gtgggcctta tctgcactga aaatccgcca cgaagctttt
    18421 cttgataatg tggaacaggc actgagacat tcatctgatg gcctgattct ccggctactg
    18481 gatatacacg aggtcgggcg tgaaatacgt cgtcagacag aacggcacag cacaccgcgt
    18541 aactggcaac cccacctgcc tgaagatgcc cagccagcgg gatatcgctg gactgacgat
    18601 gaatccgtac tacatgctcc ttcattgcat ttgcagttat tcaacactca ggtgaccaca
    18661 cagggcaacc tggttcaggc gggcggactc tggcacggaa tggtatcgat tactttacct
    18721 ccgcaaaatc tgcagacgtt taacgaactg gtccgtgccg tcccccgcgc agtcccctgg
    18781 cgcatcagaa tggatctgat gcccggcggg atgaaggctc ttaatctgaa gaaaacgctt
    18841 ctgacttaca gcagcttcat ttctgccgtt cgtccaatgt atgaatccgt tatgaccctg
    18901 gctgcaacgg atgaaaaaga acctgtctgc ataatgacca tcatggcctc cacgtgggga
    18961 aaaacacgtg aaatatgcac acgaaaccag gccattctga aatcggccat tgaaggctgg
    19021 ggcgtatgtg gcaccacgac aacatttggc gatccccgac gcgcctgggt gaataccatt
    19081 ctcgcggcat ccggaggctc cggcccggtt cccctgtatc ccccgttgtc acatgccatc
    19141 tcgcttttcc cgcttaacag ggcaggctct gtctggcgag gcaaagggaa tctgatgtta
    19201 catacagaag acggttccgc atttgaggtt ggtctggcgt catcccaaca aaataaacat
    19261 acagaactgg caccaggcga tccgggactg ggtaaatcag ttctgattaa tacgttgtcg
    19321 gaaatacaga tatcatctgc tcagaaaaac ctgcccttca ttgcctatat cgataaggga
    19381 tacagtgctc agggactggt tcagcttatt cgcgacagtc ttcctccgga aagaaaagac
    19441 gaagctgtcg gtattattct gagcaatgat ccggaataca cccgtaacct ttttgacgtg
    19501 atgtacggcg ctaaaaagcc tattacgccg gaaaagaatt tcatgtcatc tgtgctgtgt
    19561 gctctctgtg tcgataccgg taccggtcaa ccctgtaacc ccggagacac ccgacaaatt
    19621 atcaaccagc ttatcgagct ggcttttaaa gagtacggtg aaaataatcc gcgactttat
    19681 cgcgcctcaa cggaagaact ggtggattca gcactgcagg actcgggact gtatgaaaaa
    19741 catgatgcag cctggtgggc cagatcaacc tggttcgaag tccgggatat gctccacaat
    19801 gccgggtata tcatggcggc tcagcgggca cattatcagg caatgcccca gcttcccgaa
    19861 gtgtcctcga tgctgggcca taccagtctc agggatgttt tcggtactgt tcagcgtgat
    19921 ggttcaaacg aacttctgct ggactacatc cgccgcgctc tcgagcaggg acataacgac
    19981 taccccatga tatccgggta tacccggttt atgatcaacc cggaaacccg ggtcatcgct
    20041 gtcgatctga ataatgttgc cggtgacaaa acgcctgcag gcaggcttaa aaccggcatc
    20101 atgtacctgc ttgcaggtca gatcgccggt ggtgacttca ccctgccaca atacagggat
    20161 gaagtgttaa agcagcttcc acgggaatac catgaaatcg ctcttaaacg tattaaccaa
    20221 cttgatcagg aggttaagac aaaagtttat gatgaactgc ataacgcccg gggcattgac
    20281 ttcatctggg aaaatctgga tacccaggag cgtgagcaac gcaagtttgc tatccgcacg
    20341 gttctgagta cgcagtatct gagggactac ccggaaagcg ttcttaagtc agcaaatact
    20401 ctctggttac tgcgctacaa acccgaagat atccccgtac tgagggataa cttcaacgtg
    20461 cctgaattta tgctgaaacg cttcctgaaa atgccggaag gcccggcacc agatggtagt
    20521 ggtgttcctg ttctgggagt attcagggta aaaagtggca cgcttgcgag aatactcaaa
    20581 tttaccgtcg gtccactgga gctatgggcg ctgaactcat cgccaaaaga cagtgccctg
    20641 cgtaaaacac tgacaaataa gttaggttca gttcgggcac gtaagattct ggccgaaaac
    20701 ttccccagag gttctgcaac gtcattaatt gagcaccggg cgggccaaca taacagtgac
    20761 aatgttattg aagaactggc ctcggagctc atccgaaaac agggatacaa tctctgatgt
    20821 gtacaattca tatcacccca cgctgccgga gacctgccag agggcaggta tttcttaaaa
    20881 aacttgaggt aaaaggtgac agtttctcat tcactctgcc gtcacgtgtc ttcaggttac
    20941 atccggcccc caatcccaga gtgatatacg cctcggatgc cagaacacag ggaaaggtta
    21001 caggcatgcg gctgttgttg ccgtccagca cagatgagct gataacaacg gcaatatggc
    21061 aggtttctgt cccggatgaa gcaccattcg acgttcagca tgtcatccag tggatactcc
    21121 cttcaccgct ggcttcgctg atgtccgatg atacctggtt atggccatcc gttcccggaa
    21181 cgccggcact gagtgccggt gactggcctg taatggatac tgacccttcc accttacggc
    21241 gcgtcagacg tcgttctttc agcatcatcg ataaccggaa caggaaaggt tatatcacca
    21301 ggcgatatca gtgtttccca ctggccccac tacctgaaga cagaaaatac gacagccttc
    21361 ctgaccttgt gctccacccc gagcaggaga caataaaatg caaagaaaaa cattactcgc
    21421 agcccttata gctatcctga atggaacggc ataccaggct catgcttatt cagtcaccgt
    21481 cgtcgccagc cgtcccgttg aacagcaggt cgtccccaga atggaggcca tcaaaggtgt
    21541 gctgagtgac atactgagta cccagacagc cacgggtacc gctatcaatc agaactctga
    21601 aaagctggca agtgtcattg cccagaacgg acaggcgaca cgtcagcaga tgattttcag
    21661 taatgaaact cagcgcctcg aggaggcacg gaaaagcttc accgtgccag actccatctg
    21721 cagtgaatcc gcatcaggga ttgcaacaga aagcaaaagt gcctcagcat ccgcagcctc
    21781 aaaactcagt aaaggtggcg gtgtgagcaa ccgtagcatc cgcgaccgtc tggccagcgc
    21841 agcaaactct cccgtgcggg aagcttatga tggcgcggcc atacacgcca gttactgtac
    21901 agaagcagaa tatgcacgtt ttggaggaac cgccgtttgt ccttctgtgg gcgaaatacc
    21961 aggtggagac agtcaggtac gctcgattta ccacggcgca ggtacagcgg acacacctgc
    22021 ggcactgaca tgggatcaga agcaaataga tgcagcgacg gcttacatga aaaacacttc
    22081 ccgcccctct gccggtcgtg ctctgggtaa aggagaagtg aatacccaga gtgggcgaac
    22141 ctacgtcgga ctccagaacg aatacaacgg catcattgat tctgcgtcga atccccaact
    22201 gacgttgatt gctgacagca cgcctaatga aagcacacgt aaagcactgg ctgaaacact
    22261 gcagtcagac tcagcggccg cttacttcga ccaggtggct tcaccagaag caaaagcacg
    22321 gggatatatg agtacccgtg agtttgaagc ttttgaggct ggccggcgct acgccaacac
    22381 agcatacctg gtggatttac aggaaatgca gggggataac ctgctccgtg aactggtaag
    22441 aatcactgcg caaatgaact ggcagctgaa cgatctgaaa gaacagatcc gccagggtaa
    22501 tgttatctct ggccagcaac tggctctgac tgcccgccag tattatgaaa aacaactcgg
    22561 tagccttgag aaaacaatca atcaggcaaa cgcacgttga aagctgacgt cagaacagga
    22621 ctgaaaaaat gaataagtta ccggaaaatt cagaccagac gaaaaacgtc aaaaaatcac
    22681 catccataac ccgcatagcc acccggagga ttattaacat ttttgttccg ctgatggaga
    22741 ctcgcctgat aacgaattct gtacagcata tgctggaaca acaaaaacgc aggctggaca
    22801 gatttcgtca ggtaaataat aaaaaggcgc aactccacct cacgtgggaa gaagctctgc
    22861 aggccagcca tatgtcagta gacgatctgg atcgtcgttt ccgccggcgc agaacagtat
    22921 ggcgattctg ttgctggagt ctgctggcca tcgctttgtt tctcagtggt atgctctttg
    22981 ccgcctcttc attacctctt acgactctgg tacgcgccat cagcactctt gtgttgatac
    23041 tgtcaggcgt cgctctttgt gcttccagag cactgattgt tacataccgc ctctggcagt
    23101 tgcatgaacg aaaagtcagc gaacccgagc aggggacttt ccgtgatttt ctgaacgacc
    23161 gcaatggctg gcgcaatgcc acattaattg ccgtaaccag taaacaatac tgatcacgtc
    23221 cagatgccag taccagtggc atgtcgccat cccccttaat taaatcctgt gtggcctttg
    23281 cccatacgca attctattcc ggagttactg tgaaaatttt acttcgcgca ctctgtgcag
    23341 gccttgctat atcctcactg ccggcaatgg cttcagtgac ctatcaggat attgtgtctg
    23401 ccgccaccaa tcctgacgat ctgtcccgcc aggcgctggt cacgatattc ggtgatgtgg
    23461 tcaccaatcc actgtcaacc agcgacccca ccctgatcgg aagcatgttc tgggtgttca
    23521 acaatatcat tgccgtactt gctatcgtct ggtgtatctt cattagtatc cgccatgttg
    23581 tgcgttcggg gcatcaggga caggtattca gcgtcgggcg cgatattgtc ggcatactga
    23641 gtttcgtcgc aggttttctg atgatcgttc ctaccggaaa tggctggagt ctcgctcagc
    23701 tcattatgtt atggggcgca tcgataatgg gggttgggtc cgccaatgtg atggtgcaag
    23761 ttgccgctga taatatcgca aatggttatt caatgactgt acagccagtt caggcatcca
    23821 cacgaacagc agcgcgtggc atttttgaaa tggaactgtg taagtatgcg gttaacgcag
    23881 ggctaaatga ttttaatcag acggccaaat cctcgacttc actgatgaca gaatcagcta
    23941 aaaccgccag tggtaattac actgtcacag tatccaatgg cagcggtatc tgtggtactg
    24001 ccagcctgtc agtagaaggg aacggaacaa cagatcagtc gacgattgga aaattcttta
    24061 atcccttcag taaaaacgaa tacagcggtg ttatctcagc acaacgtgca gccatggata
    24121 atatgattag cgacatggac aacgctgcca gtgagtttgt gacaacattt ctggaaaaaa
    24181 gaaattctgg caacggaaca ctgccggata tcgagacacg aattcagaaa gcagccgacg
    24241 aatacgaacg tgctgtgcaa aaatcgctac ccactgataa cggagagcaa tccagaaaag
    24301 aggctcttaa atcttatctc acaacctacg ggtgggtaac gcttggcgcg tggtatcaga
    24361 cctttgcgac agctaaccaa cgtctggcag agctggctga cagagctcca gctgtaacct
    24421 caatgtcatc actgggagaa gttggtgata ctgacttatt cagtgcagtc atgagtgcct
    24481 acaagactca attacagaac accacctata caccacctct gggcactgtt acatcagcca
    24541 atgagcaacg aatggccaat gcgcaagatc ctaagtctgc cttatctgaa atgatgagct
    24601 tctttgtcta tcgaaccaac tggatagcaa cagagataag tggcacagga acaacaagcg
    24661 atcaggtcaa tccattgata aagatgaaaa atattggtga ttatacatta ggaacaacag
    24721 agattctctg gagtggatat acaacagtta gagttctcgc gacagtaggt gataactcta
    24781 ttttcggaaa ggttgccaat ttattctctg gattacctaa aggattagtt gctctgctgg
    24841 atgctgcagc tcccccaata tatttcttgt tgttcctgtt gttttgtgcg ggtttttcct
    24901 tatcaattta cctaccattt ataccattca ttttctggat gacaggcatt gggaactgga
    24961 tagtcagtgt attaatagga tgtaccgccg gacctctgtg ggctgcaaca catctcggaa
    25021 catcagaaga cagaggaagt cgggcagcct acggttatat atacttaatt gatagtatga
    25081 taaggccgcc catcatggtc tttggttttt tctttgcttc tgttgccatc atcgccgtag
    25141 gcacaatact gaatgcatta tttggcgctg ccctggttaa cgtacagttc aattcgctga
    25201 ccggtatttt cagtcttgct ggtttcttgc tgatatacgc cagaatttgt acaaccactg
    25261 tggctgccat atttgccctt caggcctacc tacctgatca tgttattaat ttccttggtg
    25321 ggcgtgaggg agccaacact cttggcagca tggcaaattc tgttaaagaa atatttattg
    25381 gaggaagtcg caatatccga catactcctg gaatgaaaac agatcgtctt aaaaataata
    25441 caaatggaag tgacgacaag gacggaataa aaggataata taaatattgc acaacctccc
    25501 catcggcatt aaaataatag atagtacgag gggaggcatt gtatgaaagc taaaaaagag
    25561 acacctgaca gatttccaac ttggtggttg ttatattatg tattaaggaa ggcttacttc
    25621 tttttaggaa tacctttctt tttattttgc gcactgacat ctacactgat gttatttagt
    25681 tctcgttatt atggtgacaa cattgaagat tatgttgtca cttttggtag ttggttctta
    25741 ttacttgcac caggtatatg gatgtattca cgtgcaaaaa caaggagaga aaagataaga
    25801 aaagttgtac aaacaataaa agagtcaggc ttctactctc cagagaaggg gtatgaagga
    25861 ttatctttaa ctcagggagc ttattttggt attgacctta aaaatggcac aatgttgtat
    25921 gtccgtatct atccaggcaa cattatggat gttatcggtt ttgacattca taatttcact
    25981 cgtactgtaa cagacgacaa aacactggaa attcatacga aatacatcaa tctgccaatg
    26041 gttcctattc catcatggtg tactcatcca gaaaccgcat caaacaccat gcacgctatg
    26101 gcaagtcgag gatatgatta ccctgtcgac tttcctcgtc tgattcagga aaagcgtaag
    26161 gagtgggagc agattgcagg cgtgcctgta gccgaagtat tttaagataa gctcccccca
    26221 taacagccgg ttcaaatgag ccggcttttg ttcattacaa cagataactt atgccgttac
    26281 cgataaattc gaccaagtgc tttaatcgca gcaattttta tttccatatc atcactttga
    26341 ctcaatctct ctaattcacg aatgatgtta tccgcctggc atcgaccttc acctaatgca
    26401 tagatcgcag caagcgtaat gtcttttgat ttactcttca tcatttcaaa caagaaagta
    26461 ttagctatcg caggaggcat gctcataatc aatcctcttc atgaaataaa caatggcact
    26521 cacttacaaa caggagtcca ctatcgcaag cgactcaact tagtaatatc tctcaatttg
    26581 aatgaaaaaa ccagatggca tgtgacaggg aaccgtccgc atgatgtccg tgaccattta
    26641 accccataaa ctgataaaaa atgaataccg gtatctcaat gcgaagaaat aatctccgta
    26701 gaatccccca tgagaaaaat atcatatggg agaaaatcgc aattatcaca gccagcgcat
    26761 tcattatttt cgctgtgttc tggcattaat tattttcaac acaaaaagga aaaaacaatg
    26821 aatctggtag acgcctttgt caaaaaagta atatccggtc catatgaaga atatggaaaa
    26881 tggtggatag atgttgaata catttcatgg ggtgttcccg ggaaaacaag actgatgttt
    26941 gagtcaaagg agcaggcact ggaagttaaa gaagggtaca aatttttaac gtgagatctc
    27001 tttttgttaa agggcaggaa gcggtaactc cctgccctta gttacgtttc caggcttatc
    27061 agtcgacaag ttcccggatc agttccagaa cagcaatcag cgcgtttgca acggcaacta
    27121 ctgccctgag aacgatgatt aacagattaa tcatccgctt ctccatcact ggaggaaatc
    27181 accgttgctt actcttacat tgcctgtcac aacggcactt tcagtgttag ttgaaaagag
    27241 acttcctcct gccagagcac tgaacacagc accacctgtt tgttcagcca ggacttaaaa
    27301 tcttgcatcc atagtcccga tggatatcac ggcagaagtg tcgccagaca gagctaacct
    27361 gcggattata aggatggttg ctatcaaatg aacagctcgc tattataaga acgttagttg
    27421 aaaagagatt tcctccggtc agggccaccc actctgtaac ggaacagtaa aaaaaccgac
    27481 ctcaccagtc ggttttttta catctgttgt ttctcaaaat ccgcccaaca tgcccgtcac
    27541 accggggaaa ctcatgctct gaatcagaac caaccatctc aactttgccg tctcctgaaa
    27601 aaactggcac attcagttcg ataactaccc ataaatacag agagaaaatc cgatgtcaga
    27661 aaagtggagt aaagaattcg actcttgctt tcaggtgggg agcatagaag aaattgcaga
    27721 tgctctcgag aaacgcagcg gaagcgagga aaatgcgtta gccatgctgg caatgacagc
    27781 cttcacactc atgacccgac gtggtatctg cgaaatgcag aacgtggccc ccgatggcaa
    27841 aggcattcgg ctggagctaa tcggcttcag ggaaaatgaa acaataccgg atacgcttca
    27901 ttgaagaaga aagggcgcaa tgaggtcagc cttcgcaaca aagcggcggc agaaaacctg
    27961 ccgccgtgtt gcccgttaac gtttaacttc gtaggctaac gttgccacaa gcactgtgtt
    28021 tccctgctgg agccggagtc cgcaaagcga atccctcacc atccagacaa aacacagaat
    28081 cgtcacacag atgacgatta acatcattaa aaacgttcgc tgtggcatca tagcccctcc
    28141 attgccttac ggcggttatc gggctactat aatgttgtct aggcattaca gtagagccct
    28201 tggttgattg ataaaaaaat ctccaggggc tttctgctgt atatggttta gttaacaaag
    28261 agagccagtg ttccactgag caaaaccagt agctattcat gcttacaatt atcttctcca
    28321 tatctgaaat aactcaaaca gcccaataga aacatattgt tacatctaag atcttgaaaa
    28381 gacctctttg agaaatcaat atgagatcac ggtatgttga taggataata tatatgaaga
    28441 aactattgat tcgattaata cctgatgcaa tatatgaggc tctggaaaag acagctctgc
    28501 attctgaacg ctccctggaa gcacaggcca gatatatcct tagttgctcc gtagataatg
    28561 aaaaacaatt aactggtggt gaacgctatc aaagagaaat aaccgcgcgt cttaatcaag
    28621 cgttatcaga agctaatgag gtcataacag ccattaatct tgttcccgca agaattgcag
    28681 aacaattagg ccatcatgac gccatagaat ccgaaaattg gttcaccgga aatgctgttc
    28741 ctagctttac agagcttgac gaattaagcg atatctttgg atgctctcca gattggttaa
    28801 agtttggcga aaatgtacct tatccaaaga gttctaaagg aaggataaac tggaatcgtg
    28861 gcggagaaaa agatatcgat gcacttttag agcctgacaa caaaggtcgt aaagtctcat
    28921 ctattcatat tttcagggta aatgaatctg gcaatatact aatactgaga gaatttgaaa
    28981 acagcataac tacagatttt ttctcaacaa acctttactt atcagacaaa gaaaaaatag
    29041 gacagggcgg attccacgac ctggtcgatt tccttgtaat tttacaatcc ctttatttaa
    29101 aatatataaa tagcaatctg ttagtgaaaa gctataacat tagccaatat gtactggatt
    29161 cctgttataa gagcggagaa aagcacccca taactatatg tagtgcaggt gagacaagta
    29221 cctggtggga agacatatgg catgaagaca tgatagctca atcgaggaat aatgagtcct
    29281 acttctggga tggtgacaaa cctcttattg atagccttca taaagaactt caaaaaaata
    29341 acagattaaa gccaaacagt gagttagata tgccactaat tggttaaaca ccaattaaag
    29401 attatctagc taaaacaaca tcgtacacgc atactgcaaa gcccttggtg agttaataga
    29461 agaattacta agggcttata aaaaatatat acccccacat tggtgacaca ttaaaatatt
    29521 ttttgttgat gtgttaccag cctgacccaa aaacaatttt taatctttcc gttcgattta
    29581 aattccagaa atggagactt tgtcatgtct tacaatcgac agcctgtcgc agaagatcct
    29641 atgcagatat ggggcgctgt tggtgtcctt cttatcctgc tgctattcgt catatggtta
    29701 ttcttgccgg aagtggttta caccagctgc ctgattcttc ataccttatg gggactggtc
    29761 gactggggac catttcataa ctatgcagca ccacgataca acctgcttgc catgaccggt
    29821 aacaacgccg ccaacataag ctatagccag tgggttaacg tcatggaaca gaccataggt
    29881 attctgtgga tgtacctctt acctgtgaca ctctggtgtt tgtgggaatg gtatcaacat
    29941 ccgggacaaa gccgtttcac ccgcagacca gtagatatta cccgactgcc acacatcttt
    30001 gcttcgttat cccctgctat cgcccctgta ctggcagatg gtgatccgaa aaaactcttt
    30061 catggtggta aacgacctga acggcgggtt gctctcaccc cggaagcatt tgtcgaacag
    30121 catactctca tcaccaatat gcagctcgat gttgcagctg caagacgatg tttcatggcc
    30181 cagttgggta aacctttaac gtcctggaaa gacatggcac cacatgaaaa agcgctcttt
    30241 gccatatttg gccttcagta tttcctggat gatcggaagg cagcattaaa gctcatggat
    30301 acactgaacc tctcatgcag aattaaaagc aaacgggaca gcgggaaatt ctgtacccct
    30361 gtttattcac tggccaaatc agctttccag agagtgatta aaagcaacgg agcacagcaa
    30421 tggctgaaac aacatcgcta cgtccgcagt gggctggtct ggctgtatgc acatgacctg
    30481 cggcttacgc cacccaactg gatttggctg aaaggcgtgg acagaacgct gttttatgca
    30541 ttacaccgcg ccaataccac gaaaggattt attgaaggcg ctggcgtggt ggccgttgca
    30601 cgtgcagagg ccgaagccat gcgttttggt cttccctgcc ctgaaccctg tgttgatgaa
    30661 gcggtggaag gcttacggcg ggatatgctc agtctcggtc ttatctggga tgaaccacaa
    30721 ccagatcgcg accggaaacg ccgcatcctg accaactggt ccctcaccga cgacatcctg
    30781 ccccgcacac ctgctacaga caacgaattc tgattatccg gagaactgaa aatgacaatc
    30841 gagtactttg caacacgcat tgcaaaacat atttccgtgc aggcaatctg gccggatggc
    30901 aggacagaaa ttattgctgt ccttccacgg gatgccctgt cagggctggt aacacgaaat
    30961 aatcaactga ttcatatagc ctttgacgcc atgggcaccc gtaacagaga aaccgtcctg
    31021 attgataaac aggagcataa cgctgaccag atacttacag ctattgattc ctgcctgcgc
    31081 cttgaatatc tgatggaacg ccgcttcagc agaacacctt tgttccgtgg cataatcgcc
    31141 agcgttgtgc tgttcgtaat ggcaacgatt ggcttctctc tgttccgcta tgttgatcgt
    31201 gtattctggg atgatacaac gcctgaagca gtacagacag caggtgagcc acgactgcta
    31261 ccccctcacc tgaatcacac tgtgccatta aatgaaggca ttcaactccc ggttcccccg
    31321 aaaaaagacg tccaggtccc ggagaaaaca atcacaagca aaaatccgga agccgcagct
    31381 gcgcgacata atctggctac tgttctgaag cggaacgccg atcggggcat gtttaccatc
    31441 aacctgtcat ccggtcatga acgaaccctg ttcgcatttc tcgatcctgc ctgtcctaac
    31501 tgcagacttc tggagccggc cttaaaacgc ctggccagcg acttcaatgt cgttatctac
    31561 ccggtttcag ttattggtgg agaggaatcc accgaccgtg tggcaccact gctgtgtgaa
    31621 aaagatgctc agaaacgtgc tgccggctgg caccgactgt attccgcaga taacggaatg
    31681 atgacgccat cagaagaaac aacgcctgca gatgaaacat gcctgaaagc agccagagct
    31741 gcgattgatg ttaacaatgt cgccttccgt aagttcggat ttgccggtac cccatgggtg
    31801 ctgtccgaca ccggctggca tctgcctaca ggaattctgc aggaaactgg cacgcttaat
    31861 ttattcctga aaaccaccga cagcgagtct ggccatgagt gaacatcgcg ttaaccccga
    31921 gcttttacac cgtacagcct ggggaaatcc tgtctggaat gcactccaga gtctcaatat
    31981 ctatggcttt tgcctggtgg caagtcttgt ggccagtttt atctggccac ttgcattacc
    32041 ggcatgtctg ttgttcacac tgataacaat gctggtattt agtctgcagc gatggcgctg
    32101 ccctcttcgt atgcccatga cgctggaatg tgctgacccg tcacaggaca gaatgattaa
    32161 aagaagtctt ttcagcttct ggccaaccct gttccagtat gaggtcattc tggaatcacc
    32221 ggccagcggc atattctatg ttggttatca acgcgtcagg gatattggca gggagttatg
    32281 gctgagtatg gacgatctga cccgccatat aatgtttttc gccaccaccg gtggcggtaa
    32341 aacggaaacc atctttgcct gggcgattaa cccactttgc tgggcaagag gattcactct
    32401 tgtcgatggc aaggcacaga atgatacggc aagaacgatc tggtatctgg ccagacgctt
    32461 tggacgtgaa gacgatgtgg aagtcattaa cttcatgaac ggaggaaaat cccgcagtga
    32521 aatcattctg agtggtgaaa aaacgcgtcc tcagtcaaat acctggaatc ccttctgtta
    32581 cagcactgag gcattcacag cggagacaat gcagtcaatg ctgccccaga acgtccaggg
    32641 gggcgagtgg cagtcccggg ccattgccat gaataaggca ctggtatttg gtactaaatt
    32701 ctggtgtgtg cgcgaaggca aaacaatgtc gctgcagatg ctccgtgaac atatgactct
    32761 cgaagggatg gccaaacttt actgtcgtgg cctcgatgat cagtggccgg aagaagctat
    32821 cgcaccgtta cgtaactacc ttcaggacgt tcccggtttc gacctgtcgc tggtcaggac
    32881 accatcagcc tggacggaag aacccagaaa acaacatgct tatctctccg ggcagttttc
    32941 tgaaactttc agcacattca cggaagcatt cggggatatt tttgctgaag attccggtga
    33001 tatcgatatc cgtgacagca ttcacagcga caggattctg atggtaatga tccccgctct
    33061 ggatacatca gcgcatacaa cgtctgctct tggacgaatg tttatcaccc agaaaagtat
    33121 gattctcgcc cgtgatttgg gctaccgcct cgaaggaact gattccgatg cactggaagt
    33181 caaaaaatat aaggggcgtt tcccttatct gtgtttcctt gatgaagtgg gggcatacta
    33241 tacggaccgt attgctgtgg aagccactca ggtgcgttca ctggattttg ctctgatttt
    33301 gatggcacag gaccaggaac gtatagaagg acagacaaca gccacgaata ctgcaacact
    33361 gatgcagaac acggggacaa aatttgccgg caggattgtc agcgagggta gtacggccag
    33421 aacgctgaaa agcgccgccg gtgaagaagc cagggcgagg atgaacaacc tgcagcgtca
    33481 ggacggcata tttggtgaat catggattga cagcccacaa atcagcatcc tgatggaaag
    33541 caagataaat gttcaggagt tgattgaact tcatcctggc gagttctttt ctattttccg
    33601 gggagaaacc gtaccatcag catcattctt catccctgac gatgagaaaa gctgcagcag
    33661 cgatcccgtt gttataaaca ggtatatcag cgtggatgcg ccacgcctgg accgtctccg
    33721 ccgtcttgtt ccccgtacta ctcaacgtcg aataccgtca ccggaaaatg tcagtgccat
    33781 cattggcgtc ctgactgcaa aaccctccag aaaaagacga aaaattcgca cagagccgca
    33841 cacgatcgtg gatacattcc agcaacgcat tgcaggacgt caggcggcta tggcaatgct
    33901 ggaagagtac gacacagata tcaacgcacg tgaaagtgcc ttgtgggaaa cagcggtgaa
    33961 tacgcttaaa acgaccacac gggaagagcg acgaattcgc tatatcacgc tgaacagacc
    34021 agaactacct gaaacaaaag aggaaaatca gatatcagtg agagcagaaa gagcaggtat
    34081 aaacctgtta acgcttccac aggacaataa ccacctgaca gggcgtcctg taaacgggtt
    34141 tcatcataaa aaaaccaacc gtccggactg ggacggaatg tactgaacag tggccgcgcc
    34201 ggattctggc gcggcctttt ttttaacctg gagaaggcgg ttcccagcgt ttatcctgct
    34261 ccggcggagg cagtgatgcg tcatcgttgc tgttcgccgc gatataatcg cggtagtagc
    34321 gttcacttcc ttcacggtga tgatccacgt ccggtcgcgt aatcctgtag tcttcgtgac
    34381 cggtacggcc tgtcacatta tgccaggcaa ccatttcagc aaagacctgg ttaaattgtt
    34441 tgtcgtttcc ttctggttca aaacccatgc gtggatcacg cccgaataat accggtgcaa
    34501 tcaaatccag tttatctgcc agcgcattgc ggtcatggtg attacggaaa acaaccctgt
    34561 caccataatc cgtacagaca aactctcccg tccgacggtc acggaaacga acactcccgt
    34621 tcttctgcaa acgggcctcg agatcaccgg tattaccgta taccggcgtt ccgcccggtt
    34681 cacaaagcac gacacagcgg tctgttgtcg ttgtgcgtga cttatcttta cggcgatcgc
    34741 gataatccca gcctttcagc tgcgatacag cagccctgtc tccctgagca gcctgaattt
    34801 cgacccactg acggtaagag ggtggatacc acttcccgtc agcaataagt ttctgccgct
    34861 catccctgat gtcttctttc agcctgatca atgcctgcat tcgctgaact tctgcaatgt
    34921 gataatgcag cttacgtaat gccggatcat cgtactgcgc acgaatgtgt gacttccgca
    34981 gacggcatgc ctgatgaatt tcccgacaac gttccccata acgcagatct ggtttacgcc
    35041 attgttcacg ccatgccaga tagcgggcac gcaggtcagc acgggcctca gctctggctt
    35101 cacgtcgctg ccggcgcagc attgggtcac gtttactgct gaccccgtac ctgtcactga
    35161 cagccagctc cgggttatag cgactttcag gttgcaccct gtcaaacaaa tctgctgggg
    35221 cactcacaaa aggtccggcc tgtggctctg cacgccccag agtcagatca ggatgaatgc
    35281 tgctggcctt caccggcgtt tgctcatgat taaaggcatc aacgacaaca agcccgtgat
    35341 gttgtttctg tagcatcagt ccctgttgtg caaacaactc atgacaacgc tgccaggtaa
    35401 actcaccctg tgtgatcatt tcttcaatct gagggctaca atggctgatg ctgtatcccc
    35461 acagactttc tttgtcagaa aacagttcgc gtttgatagc cccctgcggc cccgtacctg
    35521 tacgcatacg gtgccattct ttatcggtcg caggacagct ctccatacga cgaccacgga
    35581 agcgttctgt tggcgtaacc tggcggaaaa tatctgttgg tacaggctgc cagccaccat
    35641 cgagttgatt cacattgtcg agcgtcagca gtgaccatac gctgtcagca cgaacgggag
    35701 tctggttatg atcataaccg tcacagataa ccaggtgacc atgctgaaca cgcatccata
    35761 atcccgcttc agccagtgtg cggtgaatag cctggcagtt ttccagccgt ccttcccgtg
    35821 ccatttccgg cagacgttct ccaagatgac gacaggcata ctgctgcaga ctttctgttt
    35881 cagcgatttt ttccgggcga acatcagctg caatgaagtc cggattatag cgtcctggtg
    35941 cttccacctg gctgaaaatg tcttttggca ctggcgtgta gtcacccatt tttttcatga
    36001 gtttttctgc acaccaggag ggaccaaacg agtcaagctg tacaccttcc ctgttgcgat
    36061 cccagccatc catcaccaga aattttccgt ccatctgaga caggtacaga ccatcttccg
    36121 caagacgacg atgcagggat aaccagtcat gtacaggttc actgcgtaaa ccagcgaccg
    36181 ctgtctgcgc aacatattcg cgaaaagttt gtttttttcc gcgtgtccaa gcgttctggc
    36241 gatcacgttc aacggcagtt ttgcgaacga tacgatttcc cggcgcatgg acccagcaac
    36301 cgttatccgg agcaaaacca tgcttcagtt caagctcacg gcaggcacgg ctgagttttt
    36361 cctgactcca tgaaaggcaa ttgagataac ctgtaaccgg atgttgatga catccagctg
    36421 gcaaaagctg ctttatcgga attgccagat ctcaccgctc aacggaaaac attgcgtgat
    36481 tttcttgatg ccattcgtga tgacattatt gtcctggtaa ggacaaaggg ttatacactg
    36541 gcagatgttc gtgacacgct caaaaatgca gggtatgaag taggagagaa ggcacttcgt
    36601 gacattattc gggaagcaga atcgaaaaaa ccatcccgcc gttcttcctc taaaacagca
    36661 cctaaaaaga cagattcagg caggaaagat ggcatcgata tgaacaacaa ttgatgtgac
    36721 aatgtcacat tgtgttctga gattatgatg attacagaaa aaaaagcccc ggcataaata
    36781 ccagggcttt tctgtttaat cagactcgtg acggcaatac acctggataa tgccgtgcaa
    36841 taatatcatt cagtttttca acaaggtcag aacgcttaaa gacaatgtgt acggtccctt
    36901 tctggtagcc acgcaccgta aaatattccc cttctaacag cttaccgaca ttgtcgccgt
    36961 tcccgagaaa ttcaccatac tgcgccccga tgctgttcct gaagtcagga acgttgcgcc
    37021 cctccagcag ataaaacgga cgtaccaggt catccagtgc ctcccgtccg ctgcagtcca
    37081 gcgtcacacg cccgttactc cagcggtaca gcaggttttc aataatgatt ttcttaccca
    37141 gacggcaggg attattggtt ctgtaatccc atgacaattt ccggtagaca tcaatcagtc
    37201 cctgttcaaa tgtctgcatc ttactggcat tcagatggcg gaatgttgcc agtacattgt
    37261 ccagggtgat ttcaggacag gtgtcgctca tcagctggtt gttccactca tcacgctggc
    37321 atgaactcat gaaggtgtac atgcccgtat ctgtcatcag gcggtcccag attttccggt
    37381 cggcaagacg ggtgatattc cttttcgctt cttccggctt atccatgcga cgaacggaat
    37441 cagtcacgca gtcttcaaac ccgtaaagaa aaatatcacc tgacgcatcg agcagaatcc
    37501 tgcgcgctgt cgccaggcat tccatcgcct gcatgaacag tgcaatacct tcgttacgct
    37561 gcgcgatgat gcggtcgata gccacagacg gaataacatc tgtcgtgccg gcagtattca
    37621 ggtcagcagt gggtaaagtc tgattcatca cattgtctcc tcatcgtcac gactgccggg
    37681 tcagccaggt catttcccgg aggagcgaag ctccttctgc cgtccgggtc ttagggtatt
    37741 tccccgcagt ggacctttcc gacgggggac gctcaccacg tcacggggca gttgtccggg
    37801 ggcagacgca aaagtttttg acgcctgcgg caaaaacttt tgtggctggt acatctctcc
    37861 cctggacgac tgaaacggga tgtggtcgtg tatgtacccc cggaaagtcc gtctgcggag
    37921 aacaggccgc agtgcggcgg cagacaggca gagatagcag cgccatcagg cgcagtggcc
    37981 ttacacaccg tcaggtgtgc agggagcgcc gcatccggcg cgaccggaga aaaccgacgg
    38041 cacaacaacg gcatgcggtg gtcactggta caccactccc gcacaggtga aaacagagaa
    38101 aacagcacga catccacaat caaaaaaagt gaaatttctt cactttttca cacttatttt
    38161 tcgcgctgat ttggtgatta tggtgataaa ataaagtgaa gttttttcac tttttcaaag
    38221 gtattttatg accggctggg aactgcgaat ctggcgaaaa agcatgctct ggtcacgcga
    38281 aaaagcggcc agagaatttg gcgtgacaca gagaacatgg cacgcctggg aaaatgcgga
    38341 gcaggtggat gtcaccgtct ggaggaccac ccaggcactt tcagtccgtg acctgctgcc
    38401 tcacatgcag gggatgcaca aggcagatat catcaggcga ctggaaaatg aactggggga
    38461 aacggcagag gatgtgtgag cgtaattcac agcctggctg aatgccaggc tactgctcat
    38521 caggaaacag ttttttcagt tcttcaccgg aaagtcccgt gacctcctga atccgttccg
    38581 gcggccagcc tatatcccga agccggcagg ctatttcccg cgccacttca agagcccctt
    38641 gtttaaaacc ctcgtcgaaa cctttctgta taagacgttc tgcaatcgtc atgctgtttg
    38701 ctctgctcct gctgacaaaa aagcccggca ttttgccggg ctttgtggtt ctcatcagtg
    38761 agtgattttc ttcagttcgt catcggaaag tccggtagcc tgcttaatct gctccggcgt
    38821 catccccatt ttctgaagct ggcacgcaat agccagggct ttttcaagcg ctccttccat
    38881 gcgtccttcc atcctgcctt cctggcgacc tttttgctca agacgttcag cgatagtcat
    38941 cagtttatcc tcatgctgcg gcagacgctc agccagcgcc cgcataaatt ccggtgatac
    39001 agagtcccct gcgttaacga tgtaatgaat cagaacacgc acctgctcat ctgaaatttc
    39061 caccatgacc tgcgggagtt tgtccagcag ctccatcatg tcacgatggc ggatatgctt
    39121 catcagcagc gtcagtgcgg ccatccggcg atggttcatg atttcattgt catcaacgac
    39181 agtgatatca accagcggaa acggctttgt gtatatttta gccgcaagtg ccggattctc
    39241 aaaacagtcc agccagttca tgctgtacgg gtacgggctg cgctcaccat gatagaacag
    39301 tacaggaatc accagtggca acgttttgtg ctgctccaga tgtctctgca tcgccgccac
    39361 actgtaacgt aacatgcgga aaggcatgta cagattttcg gtgctctggt gctcgatgag
    39421 cgtataaaca tagccatctt cacccccggt ggttttcacg ctccagagga tatcgctggc
    39481 gtactgatgc aggtccggct caacaaaggt ggcgggttcc agcttcagcg tggacaggtc
    39541 gcacaactgc cggtactccg ccggaagatg cagttccaga aaatctctgg cgacgtcggg
    39601 attcgccagg aacgaccgga acgcggcatc atgcggcgtg ggcgttgtgg tgtttttctt
    39661 cttactcacg gttcctcctg gtgagatgtc tgaacgtatc ctgccgcctg cacaggcaga
    39721 cggcaagggt taatcagtgg cagacccgct caatttcaag cgcgcggagc aggtggcccc
    39781 attcgctgac tttcatggcg gggatacgca cccgcttctg ctcactgcag cgtctggcga
    39841 tatggccgct catcgccagg acgtccgggt taatctcatc ctgtgccagc tcctgacgtc
    39901 tgaagcgggt atggcggtgc aggggtttat tctcccgcag gcactcttca cgcagacgct
    39961 ttacatactc ggcggttgcg aagataattg atggtctcat ggcataatct ccatagatag
    40021 tgattgagat gccggacttc ctggcgggga agcgccggcg gagagaggca gggtttatcg
    40081 ccctgccttt tttcatttcg gacagcgacg cagtgccagc gcgagcgctt tcatcttcca
    40141 gccttccggc gtgcggtaca tcaccttcca gtagccgtcg atgaacacat aatggaagcg
    40201 cacctcttca gtgctttcag tggcaatagc ctccctgaaa gaacggtact cacgcgcctc
    40261 actgtccatc cggaaaccac tgtcacgtcc gtaataagcc gtcacaccgt ccaccgggtt
    40321 ctgaagcgta tgcccggcgg gtttgtcgca acggggaccg aggcaacgga tatcaccgtg
    40381 ttcaaccagg gcttcggcct gctgaccggt ggcatagtgg gtatgcagga tgcgaccgtt
    40441 ccaggtcggc tcaccgtgca ggtggcagta aacggtacgg aacaccccgt cgcggcaaaa
    40501 aacggaaatc gtgctgctga cagtcatgaa aacgtctcct tctgctgaat caggtcgccg
    40561 ggccgaccgg ttgctccccg gaggggcgaa agccccttct gccgtccact tcttcggggt
    40621 ttctccccgc agtggacctt tccgatgggg cgtccgtcac cacgtcacgg ggcagctgtc
    40681 cgggggcgga cgcaaaattt tttgccgggt ttatggctaa aaattttgtg gacggtatat
    40741 cttcccctgg actgctgaaa cgggatgtgg ttactccagc ccccggaaag tccacagcgg
    40801 gaaacaggcc gcaggggtgg cggcggaagg gttgactgcg ccatcaggcg cagtggcctt
    40861 acacaccgtc aggtgtgcag ggagcgccgt ttccggcgcg acaggggcgg cgcgaccgga
    40921 acggtcgctg caagcctggt ttttcagaca tcggaccgta gcggcagccc gaaaccgcgc
    40981 cagcgggttc ggcggagact ggcgggtgcg ccagcagctg cctgtcggag tcgggcctga
    41041 cgaccggatg ccgccaggca gccgggagcc gaaggtgtcc ccgcctccgg aaatcagaac
    41101 aacagaaacg acataacaga aaaatgcctc acaccatgac gggcaggaag ggcagcgccg
    41161 caggcgcggc cctcctgcat ttcaggcagg cgtaagcctg ccagggtttt ccggcggcct
    41221 ggcaggccat acccccgccg gagcggggga gtaaatcacc gacggaacac atgaccgtca
    41281 ataaaggtga aggagtccag gaataaatcc cgcgcatacg cctcatagtc aaaatagagc
    41341 gccaccgtct ccggcacgtc agccagcagg ccggtatcac tgacgaactc aaccgcaaaa
    41401 gcctcctcac tgtcagcctc gccccaccag gcatcgcgga aggtgtcaaa atccgtctca
    41461 ccggtatcat ccacccagag acgataagcc tcttcgcagc cttcatcccg tgcccggcgg
    41521 aagccttcaa cccaggccca gttgatatgg cattcagagg ccatattccc cgggaatccc
    41581 tcataatcct gaaacatcag ttcaggatcg gcttcatcct ggtgaagagc acggcaggcg
    41641 gcgaaaaagt cgcgctcatc atcaaacgtg gtcaggtcaa accagcgtcc ggcgatgctt
    41701 ccacagttgt atttgtgcca ggttccaacg tatacagcag gtgcaacaac agacattccc
    41761 gaatcctcca tagtcacatg ttctgcggca ccgggcggtg ccgtgcctcc ataaaagccg
    41821 ggcgataagg cggggatgca cagggcgcaa gggaagccgc agcctgacgc acacggctgg
    41881 cgggagttgg agggtgagac cgcgaaatct gagcgggtga acgaccggaa aatcacggca
    41941 gacgggaagg agggaccggg tgaccgcagc ggcgaagcgt acccttgcat ccctgccagc
    42001 ccggctaaga tggaggcacg gcacagcccg cacacgacgc tgaggccaca gagctgaaaa
    42061 cccacaaggc catgccccgc atggccgggt aaaagcggag ccgccggcga cttccttatt
    42121 ccggcgagtg cagaggacac gccgcatgac ctccagacca cccctgatat gaaacgatca
    42181 tgatcggcgt gtccctgcgt aaaatataca tgtctggttc accggagaat atctgtgata
    42241 tccacaagtt ttctgtcgca cagccagtcc tgcgcttcac gcaccacgct actgtcgggt
    42301 ttcaccgctt tcagcgatga tgccagtcgc ttgatcaaca taaccagttc ccgtgtctgt
    42361 atgtccggca ttgtctccgg cggagcagag ggactctcat ttccgacgcc catcccatgc
    42421 gcttccagtg tggcacggac aaaccaggcg gcatcgacca gttgctggcg aatccaggtc
    42481 agatcggcat ttctgtcacg ggctgccatt cgctcacccg acagcgcctt gctgatatgg
    42541 ctgcgcacca gattatccag gagttcctgc gcttcttccg gtgaaaagcc acctggtatc
    42601 tctgtgcgca acagtccttt cctgtacagt agctgcagca gatcaatctg cgaccgcact
    42661 gcaactatca gtacataacg gacaatattc gcactggcgg tggtttctgt atcattcagc
    42721 agttcctccg gatcattacc accggtattg cagagcaacg gcaacagagg tcgcatccac
    42781 acgggaacgt gatattgttc gtctgatttc atcatattgc gttctccagc ttgttcggga
    42841 tcatccactg ctcaagtgca cgtacctgta ccggtgcacc acgaccggcc tcaccggctt
    42901 caaaaatcag ccgccagagt ggttcctcct gccagaaaaa ccggtcaaat gatgacagtg
    42961 atggaaaacg cccgcaccac ccccagaaaa tcgtttccag atcatgctcc tcaacaacac
    43021 cagaccagcg ggacaaccag cttccgaccg tggcgggagt atgaaacgca aggtcaatct
    43081 gcgcctgagc aaaacatgtc tggtattcca gttcccgtcg ttctgcctca cgttcccgct
    43141 ggcggctgta tttctgctct gtcagcgtgg ttttctgacg acgccgctca ccttcacgaa
    43201 aaggaacgga cgggaacaga cgatgccccg catcggatgg caggggaaga acacagactt
    43261 caccaaaact ttcaatcagt ttatccacgg cagccagcca caggagacgg ttgccatgaa
    43321 attcctcctt cgtcagcgca ggagcaaaaa agcggagatc ggtcagcgaa atccggcgac
    43381 tgccattcag gcagcgaaag aaagcgctgg cataaggcca tgctgaaagt gtgcgtccct
    43441 gacgacgacg ttgttcggtg acagccacag cctgcatggc ggcctgttgc tctgcgctga
    43501 gggggatcag tgcccgtgaa cgtgcgctca tgcctgacct ccttctgcca tcaggatatg
    43561 gatgatgtcg ttaacactgt acccctgcgc atcaagtgtc gcggcaagcg ccagcgtgtg
    43621 actgatagcg acaggcgtga agatatgttg cacctgaacg acggcgaaag gttgtccacc
    43681 acaattcacc agaaccagca tccagagctg agagacgtct cccggcgaac ataatgccag
    43741 gtggaaacgt tcgtgggctg gcgtgaaacg aacctggacg gggaaaaagc ccccgaactc
    43801 actgccatac tcgccattca tcatccagtt agccggtgcg tccacttcac gcatcacctc
    43861 gtgagtgaga ttacggcgca tatcgcttcc cgcagcacgg atatcttcat attcctgtgc
    43921 gttcatggac tgcagggcat tcagggtcag ttcagttttc atcacggtat ctcccttgct
    43981 gccgccccgt ctccggcggg gcggtgtggt tgttcatcag gctgcatcag ccaggttatc
    44041 ggtgcgttca gtagcatccg tctgtggatg tggtgcacac atccagccag gcacccagcg
    44101 gttgtctttc atgtggaact ctgcatgttc agccgcatcg cctttcttca ttttctccgc
    44161 gtcccgcgca gcaccggaca gtccggcctc attcagggct gcgataatct gcggcttttt
    44221 caggtgtccg aaaaagtttg ctttcgtcgg ctgccaccag tcgcgcatgt gaaagccgat
    44281 ggcggtttcc agcgagtcca gcggactgcg tgacgtgtga ccacactcac gggtctgaac
    44341 gccgtggatg ctgcatgcag tgcagaaact gagcagggac aacagtacct cctggctgag
    44401 tgacaggaac gtcgtcatat cccgtgccca tccctccggt aacagggctg caagacgagc
    44461 tttttctgcc atcagcgcca tgaatgcggc accttccttc cccgacggcg catcgctggt
    44521 cagcgagtaa tgctcacatt ccaggcggat tctggcggga ttgctgtacg ccccacagcc
    44581 aaatacattc agacagagtg tccatgccag cagtgccaga gatttgtccg gctgctgcat
    44641 cagtgctgcc tggactgcca gcgtgcgctc tgaggacatt ttcgtcagca gtggcagact
    44701 gatttcctcc acggaggctt tctcctgcac ctgttccgtg tggtcagcgt cgtcagccac
    44761 atcatcctca ctgcgtaact gcacaccacg ctggacacat acgtttccat aacgccagga
    44821 caccaccacg ccacttccgg cacgcatctc cggcgtccac gccctgacct tcgccatgca
    44881 gtcaatcagc ttcatttctg cttccagcag gtcggattcc tcacactggt tttccagcgc
    44941 gtcgtaacgt gccatcagtt cgttcaggtg ttcttcttcc gcctccgtca gcaccgcttc
    45001 cggctccggc aggttgcggt atgcccgggc atcttcacgg cattcaccga caggctccat
    45061 gcgtccggcg caccatcccc agccctcggc ttcccgaagg tgttcagcga cagcctggag
    45121 tttttccagc agggcggcat cgagcgccac gcggtcgaca taaccgtccc cgtcatcgct
    45181 gaacaaatcg gtgcgcagtt cgtctggcga gaaggcatca gcccccacga agcggaattt
    45241 actgttcccc gccaccgcca cttcactttc ggtaatcagg ttgcggataa cccgcacatc
    45301 cggtttaccg ccccatcctg actggcaggc ggcttcaaac acctgcacct gacgcgcggt
    45361 gtcgttctcc agcgccagcg cctgacagtg ttcggtggtg atgcggtctt ctgccagcgc
    45421 atcaaggatg acgggtgcaa ggtctgccag tttcagcatt cgctggacgt ggcggggtga
    45481 atagcccagc aggtcgccag tctgcgcagg tgttttgcct tcctgcgcca ttgcgcggaa
    45541 tccggcaatc tgttcggctg ggtgcatatc ccgacgatga ccgttctcgg tcatcgatgc
    45601 agccgtcgcc agttcctgcg gaataatttt cacgcggaca ggccagtcag ccggaatgat
    45661 gccacgctct gccagcatgt tgagtgctgc cagtcggcga ccacctgcgg cgacaccgta
    45721 acggtcacct ggcagggcat gaacaaccag attctgcagc aggccgactc ccttaatgga
    45781 atcagccagt tcactgacgg actccgcaga atacggcacc gtgcgcacat tcagcggtga
    45841 tttaatcagt gaatccagcg gcacgctcac ttcctccgtc tgcgccagca gggcagacag
    45901 tgcagtttcc tgcgttttta cagatttacg gcttgctttg cgggtagttt ctggttcaca
    45961 cttcgttaca ggcataatca tccccttata attgcttatg tcctgccgga cgttcgcgcg
    46021 tccggcacct ctcttactta agaatcccga aatccctgat gtactggttc atccagtacc
    46081 cggcaccgtc ctcaaaattc caggagcgcc agatcaacgt gccatcctga cggacaacca
    46141 ggcgaaaatg cgttccctga tcatcctcga taaagacgtt ccggtactgt gcggcaacgg
    46201 cttccgcctg ttcgcgggta aaggagccgt ccggcagtcc ctgaagtatt ctgaaatatt
    46261 cgctcatcct catatctccg gtgatgcccc gcacagggcg gggcggttgt cacagtcagc
    46321 ccgatcagaa cgggatgtcg tctgaaaacc cgtaatcctc cccctccggc ggttgcggct
    46381 gaggctctgg ctgcgcggcc ttacgtccac ggcctttcgt tttcgcgcca ccttttttcg
    46441 tcgcgtcagc actctgtgac tgcccattct gctgaggctt cggttgcgcc tgagcgttct
    46501 gctgtggtgc acgtcccagc atctgcacgg tgcccgtggt cttaacaaga atttcagtga
    46561 cgtaacgggt gataccgtta tcttcccagc tacgggtgcg aagctgacct tcgatgtaga
    46621 cctgcgcacc cttgcgcaga tattcacctg ccacttccgc gagcttgccg aacagcacca
    46681 cgcggtgcca ttccgtctgc tcccgcatct cccccgtctg tttgtcacgc cagctttctg
    46741 atgtggccac ctgcaggttt gccactgcgc ccccgttggg gatgtaacgg acttccggat
    46801 cattgcccag acggtagggt agggtacgtg tggtctttca agataaatgg cgaatatgtc
    46861 ctgcttatct tgagttagat caccgttacc ccccctccga accgtacgtg cacctttcag
    46921 tgcatacggc tctccattgc tagcttattg gggataaact catttagcag aatccccgct
    46981 tgagacaggt ggttgcagga gtattcttcc cttttcctgt ctcaaatcgg gtagttttcc
    47041 gctatgtaac ttgtcgtggc aacttgcgca caaaatcatc gttttccggc ggcgtgcgat
    47101 catcattagt tgccacaggc tgggtttata tccggcctta cgacctttct tcaggtcttt
    47161 tagctttctg acatgatgta cttcgcagcg cccagtcttc gcacaatact cgcagatcct
    47221 tgcccctaat cgacgcatga tctctgttgt gcgggttgtg atatgtgcgg ttctcggttc
    47281 cgtatcgaca atcgttgttc ggttgcgatt gacatgtttg agtttgaaaa cctcaatctt
    47341 tcgggatcca tttttggcct gaacggtaat agccagatct ccattatttt gccgtagcga
    47401 tttggcgact ttattcactg aactacgtcg tttattcgcc agggtcttaa aaaggcttat
    47461 ttgccatatg aaatataatt ttgataattt atatttcatg tccagcgcca gactgtagta
    47521 gttcgccaat ccgcgcattt ccgcgttgaa agtcagaaca atttcagctt cactgagatt
    47581 ttgtagactt gaacgatgta cagccttcac attctcataa cagccatatt tcttttggtg
    47641 acaaaattta gcggctatat tgtcaggaac gaagagctgc acaattcctg tggtggatct
    47701 ccggagagcg tgccgccctt gcattttctg cttcagcaat ttctttgcat ctgcctgtct
    47761 gatttcatag cctaaaaaat taactccttc tgagatatgg cggatcctgg tctttgcttc
    47821 attaacttcc aatcccagtg aagtagtaat aaagtttctg acctgtcttg atatatgttc
    47881 agcatctttc ttcgagccga tgacaccgat tacgaagtca tcagcatatc gaacatagta
    47941 catacgcctg aagtttggat cgttcatgac ctgagatggt atagaacgtt tttcttcaag
    48001 cagtcgttca atttctttct ttatggcttc tttttctgat tcagaatcag ttgccatacc
    48061 gtctactttc tttcgtagtt tatgaatctt atgggccagc ctgttcatat ctggattcgc
    48121 tcttcgtttt gtaccgcttg tatatgcagg aattaaattc tgttctacaa aacggtccag
    48181 tttgtcgagg tatatatttg ccagtaaggg agaaattatc ccaccttgtg gcgtgccaga
    48241 atatgagcga tggtatttcc aattgtccac atatcctgcc ttcagccact gttcgattaa
    48301 tttcaggaaa ttcttatcct taattttctc tgccagtgta ttcagcaata gtttgtgggg
    48361 gatattgtca aagaaacctt tgatatcaac atccacgatc catttaactc cgttccagac
    48421 tggacgaata gatctgagcg cagtttcaca cgaacgtttt ggtctgaatc catgagagtg
    48481 ttcagagaat actggttcat aaaccctttc cagaataatt cttgctactt cttgcgccag
    48541 tttatcgtct ccacctggaa tccccagcgg tctggttttt ccgtcctttt ttgggatctg
    48601 aactctgcgt acaggtttcg ctacgtattc tctactccgc aatgtttttg ccagcttctc
    48661 tgctctttcg atagaaaagc catcaatggt atctgttgat acaccttgag tcagtgcacc
    48721 tgcattggcc cgtatattca cgtaggcttg cagccacagt ccgggctcac acgcaatttt
    48781 gtatagatta cgtactggat accctttttt tgaagcatcc tcaattccat ctaatctttt
    48841 ctcaagttca tcaggcagca ttggctaacc ctcctcttag agattgatgt atctaacaac
    48901 tgtctccctt ccccgcagca ctgtgaggct ttcatcttcc ttccggttga tttatacctt
    48961 tgctgcttcc gtataatgcg gactgccgga acccattaca ggccgacctt gagtactatg
    49021 gagactccgc taccatgctg ggtataaatc cagtttaggt agtcccgtag tttcacggtg
    49081 tggagatgcg atgtacttag aatgtctttc gatcgtttaa ctatttcatc aatagctggc
    49141 ccgttagagt ggtcacaatc aacgtcggac ggatttcttg tgctagtacg ggaataacgc
    49201 ttcaatcgtt ctgcgttatt ggtttcctgt ttcccacata gatctcgact tcaacaaacc
    49261 cagtttatcc atatacatct tgcattgaat ggtgatactt ataacgattt ccatattcac
    49321 cacccttttc tgtggatgct atgttcacct ctccttttca ggatctccga gcctgttacc
    49381 aagcaaggct aaccacagta tgcaatggct ataccactaa gccatgttgc ttttcaggca
    49441 acactccata aaacaccgcg cttcaacggc gcaccccgac gaggatgacc ttgttgatac
    49501 cacgtgcact cataagaacc tcctgactga aagatgtgag ataaaccgga taccggcgac
    49561 gggacgttca tgtgcccctt cagaggagcg aaagctcctc ctgccgtcca cttcttcggg
    49621 gtttctcccc gcagtggacc tttccgatgg gggcccgttt accgcctcac gggacagctg
    49681 tccgggggcg gacgcaaaat tttttgccgg gttcatggct aaaaattttg tggccggtgc
    49741 atctcaccct ggacagatgg aacgggaggg ggttacgtct gccccccgga aagtccacag
    49801 cgggaaacag gccgcagggg tggcggcagg agggaggact gctgcgccat caggcgcagt
    49861 ttcctcacac accgtcaggt gtgcagggag cgcaggtcac ggcgcgacag gggcggcgcg
    49921 accgtaacgg tcgctgcaag ccc
//
LOCUS       DAJTXQ010000012        49641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000012 DAJTXQ010000000
VERSION     DAJTXQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.012"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..340
                     /locus_tag="OO515_000673"
     CDS             <1..340
                     /locus_tag="OO515_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6574687.1"
                     /translation="QIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVI
                     KLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAI
                     TDFMTARGYH"
     gene            complement(389..1888)
                     /gene="pitB"
                     /locus_tag="OO515_000674"
     CDS             complement(389..1888)
                     /gene="pitB"
                     /locus_tag="OO515_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6574688.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2180..4039)
                     /gene="gss"
                     /locus_tag="OO515_000675"
     CDS             complement(2180..4039)
                     /gene="gss"
                     /locus_tag="OO515_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6574689.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4244..5110
                     /gene="yghU"
                     /locus_tag="OO515_000676"
     CDS             4244..5110
                     /gene="yghU"
                     /locus_tag="OO515_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6574690.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5233..5481)
                     /gene="hybG"
                     /locus_tag="OO515_000677"
     CDS             complement(5233..5481)
                     /gene="hybG"
                     /locus_tag="OO515_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6574691.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5494..5835)
                     /gene="hypA"
                     /locus_tag="OO515_000678"
     CDS             complement(5494..5835)
                     /gene="hypA"
                     /locus_tag="OO515_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6574692.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5828..6316)
                     /gene="hybE"
                     /locus_tag="OO515_000679"
     CDS             complement(5828..6316)
                     /gene="hybE"
                     /locus_tag="OO515_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6574693.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6309..6803)
                     /locus_tag="OO515_000680"
     CDS             complement(6309..6803)
                     /locus_tag="OO515_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6574694.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6803..8506)
                     /gene="hybC"
                     /locus_tag="OO515_000681"
     CDS             complement(6803..8506)
                     /gene="hybC"
                     /locus_tag="OO515_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6574695.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8503..9681)
                     /gene="hybB"
                     /locus_tag="OO515_000682"
     CDS             complement(8503..9681)
                     /gene="hybB"
                     /locus_tag="OO515_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6574696.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9671..10657)
                     /gene="hybA"
                     /locus_tag="OO515_000683"
     CDS             complement(9671..10657)
                     /gene="hybA"
                     /locus_tag="OO515_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6574697.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10660..11778)
                     /gene="hybO"
                     /locus_tag="OO515_000684"
     CDS             complement(10660..11778)
                     /gene="hybO"
                     /locus_tag="OO515_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6574698.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(11967..12254)
                     /locus_tag="OO515_000685"
     CDS             complement(11967..12254)
                     /locus_tag="OO515_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6574699.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12373..13260)
                     /locus_tag="OO515_000686"
     CDS             complement(12373..13260)
                     /locus_tag="OO515_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6574700.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13417..14457
                     /gene="gpr"
                     /locus_tag="OO515_000687"
     CDS             13417..14457
                     /gene="gpr"
                     /locus_tag="OO515_000687"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6574701.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14497..14991)
                     /locus_tag="OO515_000688"
     CDS             complement(14497..14991)
                     /locus_tag="OO515_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6574702.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15182..16066
                     /gene="yghA"
                     /locus_tag="OO515_000689"
     CDS             15182..16066
                     /gene="yghA"
                     /locus_tag="OO515_000689"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6574703.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16226..16651)
                     /gene="exbD"
                     /locus_tag="OO515_000690"
     CDS             complement(16226..16651)
                     /gene="exbD"
                     /locus_tag="OO515_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6574704.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16658..17392)
                     /gene="exbB"
                     /locus_tag="OO515_000691"
     CDS             complement(16658..17392)
                     /gene="exbB"
                     /locus_tag="OO515_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6574705.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17644..18831
                     /gene="metC"
                     /locus_tag="OO515_000692"
     CDS             17644..18831
                     /gene="metC"
                     /locus_tag="OO515_000692"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6574706.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            18971..19630
                     /locus_tag="OO515_000693"
     CDS             18971..19630
                     /locus_tag="OO515_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6574707.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19670..20569)
                     /gene="yqhC"
                     /locus_tag="OO515_000694"
     CDS             complement(19670..20569)
                     /gene="yqhC"
                     /locus_tag="OO515_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6574708.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20763..21926
                     /gene="yqhD"
                     /locus_tag="OO515_000695"
     CDS             20763..21926
                     /gene="yqhD"
                     /locus_tag="OO515_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6574709.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22031..22858
                     /gene="dkgA"
                     /locus_tag="OO515_000696"
     CDS             22031..22858
                     /gene="dkgA"
                     /locus_tag="OO515_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6574710.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23058..23984
                     /locus_tag="OO515_000697"
     CDS             23058..23984
                     /locus_tag="OO515_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6574711.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24035..24292
                     /gene="yqhH"
                     /locus_tag="OO515_000698"
     CDS             24035..24292
                     /gene="yqhH"
                     /locus_tag="OO515_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6574712.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24335..26554)
                     /locus_tag="OO515_000699"
     CDS             complement(24335..26554)
                     /locus_tag="OO515_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6574713.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26665..28077)
                     /gene="ftsP"
                     /locus_tag="OO515_000700"
     CDS             complement(26665..28077)
                     /gene="ftsP"
                     /locus_tag="OO515_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6574714.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28152..28889)
                     /gene="plsC"
                     /locus_tag="OO515_000701"
                     /gene_synonym="parF"
     CDS             complement(28152..28889)
                     /gene="plsC"
                     /locus_tag="OO515_000701"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6574715.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29122..31380)
                     /gene="parC"
                     /locus_tag="OO515_000702"
     CDS             complement(29122..31380)
                     /gene="parC"
                     /locus_tag="OO515_000702"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6574716.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(31926..32408)
                     /locus_tag="OO515_000703"
     CDS             complement(31926..32408)
                     /locus_tag="OO515_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6574717.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32461..32853)
                     /gene="ygiW"
                     /locus_tag="OO515_000704"
     CDS             complement(32461..32853)
                     /gene="ygiW"
                     /locus_tag="OO515_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6574718.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33005..33664
                     /gene="qseB"
                     /locus_tag="OO515_000705"
     CDS             33005..33664
                     /gene="qseB"
                     /locus_tag="OO515_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6574719.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33661..35010
                     /gene="qseC"
                     /locus_tag="OO515_000706"
     CDS             33661..35010
                     /gene="qseC"
                     /locus_tag="OO515_000706"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6574720.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35120..35701
                     /gene="mdaB"
                     /locus_tag="OO515_000707"
     CDS             35120..35701
                     /gene="mdaB"
                     /locus_tag="OO515_000707"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6574721.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35732..36046
                     /locus_tag="OO515_000708"
     CDS             35732..36046
                     /locus_tag="OO515_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6574722.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36094..37986)
                     /gene="parE"
                     /locus_tag="OO515_000709"
     CDS             complement(36094..37986)
                     /gene="parE"
                     /locus_tag="OO515_000709"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6574723.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38015..38596)
                     /gene="yqiA"
                     /locus_tag="OO515_000710"
     CDS             complement(38015..38596)
                     /gene="yqiA"
                     /locus_tag="OO515_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6574724.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38596..39423)
                     /gene="cpdA"
                     /locus_tag="OO515_000711"
     CDS             complement(38596..39423)
                     /gene="cpdA"
                     /locus_tag="OO515_000711"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6574725.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39448..39870)
                     /locus_tag="OO515_000712"
     CDS             complement(39448..39870)
                     /locus_tag="OO515_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6574726.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39871..40500)
                     /gene="nudF"
                     /locus_tag="OO515_000713"
     CDS             complement(39871..40500)
                     /gene="nudF"
                     /locus_tag="OO515_000713"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6574727.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40705..42186
                     /gene="tolC"
                     /locus_tag="OO515_000714"
     CDS             40705..42186
                     /gene="tolC"
                     /locus_tag="OO515_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6574728.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42334..43005
                     /locus_tag="OO515_000715"
     CDS             42334..43005
                     /locus_tag="OO515_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6574729.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43011..44171
                     /locus_tag="OO515_000716"
     CDS             43011..44171
                     /locus_tag="OO515_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6574730.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44209..44997)
                     /gene="ygiD"
                     /locus_tag="OO515_000717"
     CDS             complement(44209..44997)
                     /gene="ygiD"
                     /locus_tag="OO515_000717"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6574731.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45140..45913
                     /gene="zupT"
                     /locus_tag="OO515_000718"
     CDS             45140..45913
                     /gene="zupT"
                     /locus_tag="OO515_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6574732.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(45971..46141)
                     /locus_tag="OO515_000719"
     CDS             complement(45971..46141)
                     /locus_tag="OO515_000719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574733.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46402..47055)
                     /gene="ribB"
                     /locus_tag="OO515_000720"
     CDS             complement(46402..47055)
                     /gene="ribB"
                     /locus_tag="OO515_000720"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6574734.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47159..47307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47429..47719
                     /gene="ubiK"
                     /locus_tag="OO515_000721"
     CDS             47429..47719
                     /gene="ubiK"
                     /locus_tag="OO515_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6574735.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48002..48553
                     /locus_tag="OO515_000722"
     CDS             48002..48553
                     /locus_tag="OO515_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6574736.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48613..>49641
                     /locus_tag="OO515_000723"
     CDS             48613..>49641
                     /locus_tag="OO515_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6574737.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGPVYISMT"
BASE COUNT        12451 a        13161 c        12318 g        11711 t
ORIGIN      
        1 ccaaattcct ggcgcttacg atggtacggt gttcccacct aacgttgaag gtggtcgttt
       61 tgtctcatgg ctggcaagcc aggaacgtaa agcgtttcac tggatggcga gccataagcc
      121 tgatctggtc atcaaactca atgttgacct tgaagttgcc tgtgcacgta aacccgacca
      181 taaacgggaa tcgctggcga ggaagattgc cataacgcca cagttaacct ttggtggtgc
      241 acaactggtt gatatcgatg ccaatcagcc actggaacag gtgttggttg atgcagaaaa
      301 agcgattacg gattttatga ccgcgcgtgg ttatcactag tcaaaaatgg aaatgcccga
      361 tcgccaggac cgggcatttt caggaaggtt aaatcaactg caatgctatc cagtacagcc
      421 caccagaaag aaaaattgcc gccggtaaag taaacaccca cgccatcagg atgctggtta
      481 ccgttttacg ctgtaaccca ccgccgtcca ccaccatcgt ccctgctacc gcagacgaga
      541 ggacgtgtgt tgtggagaca ggcatcccaa tataactggc aaggccgata gacactgccg
      601 ccgtcatttg tgccgccatg ccttgcgcat acgtcatgcc gcgcttacca atcttctcac
      661 cgatggtcat cgctacacga cgccagccaa tcatggtgcc aatgccgagc gccagtgcta
      721 ccgccatgat gatccacacc ggagcgtact caatggtgct taacatatcg ctgcgcagtt
      781 ttttcagcag gttctggtct tctttactga cgcctggcag tttcgccagc ttcgcggagg
      841 tatcagagat gcacagcata atgcggcgca gctggctgcg ctgactcacg cttaacggct
      901 cgtaactttc catattgcct ggcagcatcg ttttaacgcg cgcaatcgca tcaaaggtat
      961 ttgccggatg acagtgaaac tctgttactt gcgtgccatc agtcgatgct gcaggcaatg
     1021 gaggttccat cgcaatcaac ttctgcggca gttcaggatg ctgttgcagg tagtgttcga
     1081 agttggtgac ggcatcgcgg gtacgggtaa tttcatagcc ggacgcgttc atattgacga
     1141 cgaagccagc aggggcaatc cccaccagta ccagcatcac caggccgatc cctttttgtc
     1201 cgtcgttcgc gccgtgcgaa aacgccacgc ccgcagcgga aacaatcagc gcaatacgcg
     1261 tccagaatgg cggtttacgt ttgccttttt tctttttgcg atcttccgga atgcggtgaa
     1321 tacggtcacg ctttttcgtc ccgctccagt agcgtcgcag caggaatatc aggcctcccg
     1381 caatgaccag gccgacgata ggggaaacaa tcagcgagga gaaaattttg gtcacttcac
     1441 gcaggtttaa cgcatccatc accgatgagc cggttaacag cgcgttggtt aaaccgatgc
     1501 cgataatcgc accaatcaag gtgtgcgaac tggaggccgg taaaccgaag aaccacgttc
     1561 ccaggttcca gataatcgcc gccagcagca tggaaaagac catcgccagg ccgtgggttg
     1621 accccatatt cagcaacaaa tcggttggca acatatggac aatggcatag gcaacgctaa
     1681 gtccgcccaa taacacgcca aaaaagttaa aaaatgccgc catcaccaca gcaagttgtg
     1741 gttgcatggc acgagtataa ataacggttg ccaccgcatt cgccgtgtca tgaaaaccat
     1801 tgattgcttc gtagaacaac acaaatgcca gagcaagcaa taacaaaagc cctgtgtata
     1861 tatcaaggcc aacaaataaa tttagcatat attagattac gccattttga atttacgaac
     1921 ggacgcatta tcagtgactt taacggcatg ggcaaagtga aatatcattt tttaacgata
     1981 aaaagagtgt tatttcttgt gtatcagaca ataagcattc attcaaaacc tttagaaata
     2041 atcaaaacac aggtattcca ctggttaata tattcacata tgaaataaat aaatattaga
     2101 acatataaat attgaatatt ttgattaatc cccgtactga ttattcttca taatcagtac
     2161 ggggttgcta caacatgtat tactttttca ccacaattaa cggttcaata tcactctctt
     2221 ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg ccgcccacgg
     2281 tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac agttgctgat
     2341 agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct tcatgatggc
     2401 tgaccaggtc gatattgcta ccacagcgac cggcaatcgg tttcactgcg taacctgttt
     2461 tcaccaattc atcattaaca gtgaaatcgg tatccagcag gtaacgatgg tgcgggaaca
     2521 gcgaccagag gatcggcaga atcgctttgt tgccggggat caccgtccac agcggctcaa
     2581 agactagcac ttccgggcgc agcaatacgt cgataagacg cacttcgttt tgcggatgac
     2641 cggtacggat tggcaccgca gcaaactcac ggtcgctaac ttcacgaatc tgatcaaacg
     2701 cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc ccatcaatca
     2761 gttgcccggc agcatcccag cccagttcat ccaatccacg caagatacgc gtttcaaagc
     2821 ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg atatctttgt
     2881 cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca gccaattcgt
     2941 taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc gcccaacgtt
     3001 cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc ggcgttgtac tcgtaaacct
     3061 tcaggccacg ctcatccatg cagaaatcca tacgaccagt gatcatatgg tgacggcgac
     3121 gctgccagga gagacgcaaa cgtggccaga ggattttcgg gatgtcgaac agcgccagca
     3181 ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata catcaggtgc agctcgttgg
     3241 tggctttaat tagctcctgc tcggcactct cggtaatggt gtagtaatga taaggatcct
     3301 gattgatcac ctgaccgttg gcctgcacat aggcgttttg cagcggatct ttttcatcca
     3361 gccatttacc gtcaaactgg cctttgtttt ccaggcgcgc tccgctgatt ttcagcagct
     3421 cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg atcatccagc
     3481 ccagaatggt ggtgtcatca aaagtgtctt tcagggtata gcagccgttt tcgaccacca
     3541 tctccagctc acgcgtccac tgttgccctt gcggcaacgg ggaatgaatc acgttctgtt
     3601 ccgcaatacg gactttgttg ccatgcaatt gggtaatgat ggcgacatgg ccagtgtctt
     3661 taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca cgcggcgagc
     3721 cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttctcgc aggaagcgca
     3781 gcgagaaaat ctcccacgcc atacccacgt cagtaaagac cacaccgtaa ttcagaaaga
     3841 gaaaacggcg agcaaattca acgcattgcc acttgtggcc catatattcg tcgtcgatat
     3901 agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg taatctgaag
     3961 agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg gcatcctggc
     4021 tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag ttggcgtaat
     4081 tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga ctaatcatac
     4141 acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac agcaagtgac
     4201 ctgctacact gcttcaacac taccactcag aaggcaactc actatgacag acaatactta
     4261 tcagcccgcg aaagtctgga cgtgggataa atccgctggc ggcgcgttcg ccaatatcaa
     4321 tcgcccggtt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac acccattgca
     4381 actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg aggagctgct
     4441 ggcgctgggc gttactggtg cagagtacga cgcctggctg attcgtattg gcgatggcga
     4501 tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg cgctgcgcga
     4561 tcatacgcat aatccgccga tccgcgtgtt tgaatctggt tcgatcctgc tttatctggc
     4621 ggagaaattt ggctacttcc tgccgcagga tttggcaaag cgtactgaaa cgatgaactg
     4681 gctgttctgg ttacagggcg cggcaccgtt cctcggcggt ggttttggtc acttttacca
     4741 ttacgcgccg atgaaaattg agtacgccat caaccgcttt accatggaag ccaaacgtct
     4801 gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcgggcg atgagtacac
     4861 cattgcggat atggcgattt ggccgtggtt tggcaatgtg gtgttaggcg gcgtgtatga
     4921 tgccgcagag tttcttgatg cgggcagtta taagcatgta caacgctggg cgaaagaagt
     4981 aggcgaacgt ccggcggtga aacgtgggcg tattgttaac cgcaccaacg gaccgctgaa
     5041 tgagcagttg catgagcgcc atgacgccag tgatttcgag acgaatacgg aagataagcg
     5101 tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca ggccggataa ggcgttcacg
     5161 ccgcatccgg caatcgtgca caatgcctga tgcgacgctg ccgcgtctta tcaggcctac
     5221 gaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg cgtctaatgt
     5281 ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta ccagcaccca
     5341 ctggcccagt agatcggcag ggttaccttc acaaatcagg gcgatattca cgtcgcgctt
     5401 gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag ccagcacctg
     5461 gcctggaacg ccaatacaca ttattaactc cggttattca acttcaatac ttttgacgat
     5521 cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga cagagcggac actgcgcatc
     5581 gtgctgatga atctccacca cctggctgca atcccagcac caagcctggg cgggtttata
     5641 gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt caaaactaaa
     5701 acggacggcg ctctcctcaa cgcaggagag cgcgccaatt tccagccaca cggcggtgac
     5761 gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcgc tctggcaaag
     5821 agacaactca tgcattttcg ccactcctgc gaccaaacag cagggcgcga cgccctgcgt
     5881 gtggtacatc cggattcgtg actggcagcg aaaggatcat tcgtgcgcag tcatccgtca
     5941 ggcgttggcc ctcttcaatc gacatgctgt gcgaaagcgg cgacatcagc gaacaggaga
     6001 gatattgcga aacaccgtcc agttcgccaa cggtaaaggt catagtgcca tacggcagtt
     6061 gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg ccggggaaga
     6121 taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct tcgaacagcg
     6181 taaaatcaga aacatacacc ggcattgaag gatgcagaaa agagagatcg tgcatcgaac
     6241 gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt cggggaggtc tggaaacctg
     6301 ctatctcttc agtcatgaat cgcctcccgt gggatggctt ccacgcctga ttcgcgcagc
     6361 gcagccagaa cctgctcaag cgcaggttca atcattgctt caaccgtcgg cgttaagccg
     6421 atgtgtggct ccagcgattc cgggatcacg ccgaccaggg tcagcttttt cggaaactca
     6481 ccggtgaagc gcagggccga caatacgtcg gccaggccaa gctgatgcgg agagattttg
     6541 ttggtaaaca acgccggaac ttcttcatcc cgcaggatca tcatcgttcc cggcgtgttc
     6601 tttttcgaca caatggcatc cgcaataatc agatgatcgc gatttgccat gtcgccaagc
     6661 agctccattc ccgccgtgcc gccatcgagg atctcaacat aatccggcag aatgtatcgt
     6721 tgctctaacg cttcgacaat ccgcacaccg atggcttcat cggtcagcaa aatattgccg
     6781 acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg ttgccgtcgg
     6841 catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg gtacgcacca
     6901 cttccagcgg tttattcgga tcagcaaccg gtgtacccac cagcgactgc tcgtaaggac
     6961 cgacgtcatc attgaagtta cgcggaccag agttccaggt tgatggaaca actgcctggt
     7021 agttactgat aataccgtct ttaataacca tccagtgaga gagcataccg cgcggcgctt
     7081 cgaggaagcc aacacctttg aactcacccg ttgccggaat gttcggtttc acaaaggtgg
     7141 tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttgattttgc aggatatcct
     7201 gcaattcgca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag tgcagctgcg
     7261 ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca ttcagtttgt
     7321 tctgggtaga ttcgcgacct gccgccagtt tcaccagcat attagccagc ggacccactt
     7381 ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga atatttcccg tcgtcagacc
     7441 aaccatcata agccggaatg gtggtacctt cccacggtgc ctgcggcgct tcgtctttat
     7501 accaggagtg cttcgcgctt tcctgaatgc ctttgatcag gtattcatcg gaatgagaag
     7561 tgatcggacg atacgaggac agatccgcat tctcaatgta gccgcccggg aacaggaagc
     7621 tgccgttttt actgtcggtc gggaattccg gcacgctcag gtagttcacc gcacctttac
     7681 cgcgcgtcag ccattccggg tagaacgcgg caataactgc ggtatcaacc ttataaacct
     7741 gttcaacaaa gtcgctcagc ttgtcgatga aagacttgat gtacatcagg cgctcaaggt
     7801 tcagcacgcc caaaccgtcg aggttgatcg ggttcgcgac accacctacc gccaggttct
     7861 gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg gttagcgtca cgctggcact
     7921 ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc agtttcatcg
     7981 ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg accgctggca accagatctt
     8041 tgatcttgtt ctgaacttta gtgaactctt ccggactgtt cagatgccag gtcgaaacgc
     8101 ctttcagcat ttcggaggct ttggttgggt cagcttgcag tgcagaagtg atgtccaccc
     8161 agtccagcgc cgaaagctga tagaaatgaa cgatatggtc atgcgtggtg tgcgcagcca
     8221 gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagc gcactttctg
     8281 ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac gccacagata cgttgcacaa
     8341 tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg cgccacatgg
     8401 taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg atgcgtaaat
     8461 gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt tgctcgcctc
     8521 atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac gaatgagtac
     8581 gatgtaagcg caaatctcaa tagccacaaa accaatagaa atcaacagtt cttcccaggt
     8641 cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc agcgaatagg tcagacgcca
     8701 ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac agcatgcggg aatcattacg
     8761 cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt caatccagaa
     8821 catcacggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat agataagctc
     8881 gccaaagcgc agcacgatga aaatcgccag caacacactg atggtgttgg tcagcttgac
     8941 gaacagactc ttttcatccg gaccgttgcc acgcagaccc gcctgcacca gcgaaccttc
     9001 aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca gcggcaacat
     9061 ttcatagctc tgccacaacg gatgcacctt gtagcccgcc gagatcatca gtgaccccat
     9121 tgaagactgg tgcatggtcg gcaacagcgc gccaagtgca atgatgaaga acataacctt
     9181 gttcagacgc ttgagagaaa ccttccagcc cagacgttca aacagtgccg gagcaaactc
     9241 cagtgccatc acgccgatat agatggtcat acagaccgcc gtctcgaaca gtaccgagtt
     9301 cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac ccacgtcgat
     9361 agtgatcgac aagccaccca gtgagtaacc aaacagactc gccaacagcg ccggacgcac
     9421 cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg cccagccgcc
     9481 acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc cacacgcccc acgggaagcc
     9541 gccgttcagg tcagagacag agcccagacc gaacaccaga cgcttcacaa tcaggagcat
     9601 acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt tgccgcccag
     9661 cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt ttggtgttgc
     9721 gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg cctttataca
     9781 gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt ttcggcaggt
     9841 cgagattttc ataaggcaca cccgtcagta ccagaacctg agtaccgccg ccctctttct
     9901 cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg ccagatttca
     9961 gcgtctgacg cggatagtgg tattcgctgc caggcttcag cgccagacgt tttttcgcct
    10021 ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc cgccgggcac acttctacgc
    10081 agccaggcag accgccttta tcgagacgtt ccacaccttt ctggttgcac agctcgcact
    10141 tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg cacgttgtac ggacaggcga
    10201 ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta atggacaatg ccggttttcg
    10261 gatctttttt cagtgcagag accgggcaca cagagacaca gttcggatcg acgcagtgca
    10321 tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg acccctgtgc
    10381 cgctggtcca cacctgaatg atgttattgg tatacggcga cagtttgtcg ttgttcgacc
    10441 aggtctgttc cccttgcggg ttacgttcag ggaagttgat atcctgacac ttggtgacgc
    10501 aagcctggca gcctacgcac aaggtcgagt cgtacaacat ccccagcgat cccggaattg
    10561 gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc agcaatgccc
    10621 cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc gtgagtcagc
    10681 gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga caccggcaac
    10741 cagcccaacc acaccgccga gcaaaccaat agcgcctgca gagacgttgc cgccctcttt
    10801 agcgttaaca tccggtttct gtgaacgcgg agtctgattt tcgacgttgg caagctgatg
    10861 gatgccttta tggaagccga taccttcttc gttacagcca tagcatgggt gaccaatcgc
    10921 taccggccac acgccgccaa catcgcagaa ttgcagcgtt gagcagttgc cgtaagtttc
    10981 tggcccttta cagccgaggt ggtacaggca ccagccttca cggtggcctt catcaccgaa
    11041 ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt cgtgaatcag
    11101 acggccatag gcgaaggtcg gacggttttt gtcatccagt ttcggcggtt tgccgtaagt
    11161 gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc ccggaatatt
    11221 gataacggtt ttgcctggca gaacttcttg caggctgact gcgccagttg ggttaactcc
    11281 agctgcggca acaccgcccc acgcagagca ggaaccgata gcgataatcg ctgctgcgcc
    11341 ttctgccgct ttgcggatgt gatccacaat cggctcacca gcaaccatgc aataaatacc
    11401 gttatctttt aatgggatgg aaccatccac aaccaacaca tactgccctt tgtacttctc
    11461 aagtgcgtta tgtttgttct cttcgacctg atgaccgaag gcggcggaaa gcacttcgtg
    11521 atactccaga gagatagtct ccagcacgag gttttctacc gttggatgcg ttgcacgaag
    11581 cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg gcggacgctg
    11641 cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc ccatggtggc
    11701 ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag aatggatgag
    11761 ggtgttatct ccagtcattt atagttattc cgttgcgaag acctggcata tattttgcct
    11821 caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat tatggttatc
    11881 gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag tgtaattaat
    11941 taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc cataaaaaag
    12001 cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc tttatccagt
    12061 ccatagcggc gcgtcagaat acccgcttcc caggcactaa gctggcgatc tccggtcttt
    12121 tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt tataatcggc acagaatttt
    12181 gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa gcctttacca
    12241 aaatggttat tcatcgttca cctcattcgc tatatatcgc catatatagc gatattttag
    12301 gcaaagaacg acttttgcgc acgctatttt ttcatgcttg tttaagacgt aatccagtaa
    12361 gagataaacc tatcaggaga gatatttatc gaaccatttc agtgtccttt gccaggcaag
    12421 atcggcggca gatttgtcat aacggggcgt ggaatcatta tggaatccgt gattaacccc
    12481 cggatagata tacgcctcat aaaccttatt attggctttc aacgccgcct cgtaagcagg
    12541 ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg agtagtaaag gcgcttcaat
    12601 cttcgccaca tcggcagtgg gtgcctgacg accataaaac ggcaccgcgc aggccagttc
    12661 cggatacgcg acagccgccg cgttcgatac gccaccgcca tagcaaaatc cggtaatacc
    12721 cactttgcct gccgcttgtg gatagcgttg cataaactca attgcggcaa agaaatcatt
    12781 catcagtttg gttggatcaa cctgctgttg cagctcacga cctttatcat catttcccgg
    12841 ataacctcca acggaactta agccgtcagg tgccagggcg atataccccg ccttcgccac
    12901 tcgccgtgcc acatcttcga tatacggatt cagtccacga ttctcatgca ccaccaccac
    12961 ggctggcgtt ttgccgctca tttttgcggg tttcaccaga taaccccgta cctcgccgtg
    13021 accatttggc gaaggataca tgatgtactc agcaacaatc tccgggtcgg taaactctac
    13081 ctgagtcgcc agcgcataat ttggcttgag caaatcaaac aacgctaatg ccgtcatccc
    13141 gcccaccgca tacttcgccg caaggttgag gaactcacgt ttcgaaattt tcccgtgagc
    13201 gtaatagtcg tagtaatcca acaactcttg tgggaaatct ttggcggtta aacgcggcat
    13261 cgttgcactc ctcagttggt gtttttttaa gcaaagcata agcacgtgtt tttgcccagt
    13321 ttttcgtcac tctgtgagcc agactacggg atacgcgctg gcgaatcgct aaactagaaa
    13381 cattgtttcg aaattgaacg gtggaaagga gaggtcatgg tctggttagc gaatcccgaa
    13441 cgttacgggc agatgcaata ccgctattgc ggaaaaagcg gtttacgcct gcccgcgtta
    13501 tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc tggaatcaca gcgtgcgatc
    13561 ctgcgtaaag cgtttgattt gggcattacg cactttgatt tagccaacaa ttacgggccg
    13621 cctccaggaa gcgcagaaga gaactttggt cgcctgctgc gggaggattt tgccgcttat
    13681 cgcgatgaac tgattatctc taccaaggct ggctacgata tgtggcccgg cccttacggc
    13741 tctggcggtt cacgtaaata cctgctcgcc agcctcgacc aaagcctgaa gcgtataggg
    13801 cttgagtatg tcgatatctt ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa
    13861 accgcctctg cgctggctca tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc
    13921 tcttactcgc cagagcggac gcaaaaaatg gtcgagttgc tgcacgagtg gaaaattccg
    13981 cttttaattc atcaaccttc gtacaattta ctgaaccgct gggtggataa aagcggcctg
    14041 ctggataccc tgcaaaataa cggcgtgggc tgtattgcct ttactcctct ggctcaggga
    14101 ttgctgaccg gaaaatatct caacggcatt ccgcaagatt cacggatgca tcgtgaaggg
    14161 aataaagttc gtggtctgac gccgaaaatg cttaccgaag ccaacctcaa cagcctgcgc
    14221 ttattgaatg aaatggcaca gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg
    14281 ttgctgaaag atgatcgcgt gacgtcggta ttgattggtg ccagccgcgc ggagcaacta
    14341 gaggagaacg tgcaggcgct gaataatctg acatttagca ccgaggagct ggcgcagatt
    14401 gatcagcata tcgccgatgg cgagctgaat ctgtggcagg cgtcgtccga taaatgacct
    14461 gttaataacg ggcgcgcgaa ccgcgcccga atcagatcag tgattatgac gagtcagtcg
    14521 gtcaagatag cccatcacaa atgcagaaag cacaaacgtc agatggataa tgacgtacca
    14581 catcagtttg ttatccggga catttttcgc atccataaag acgcgcagta agtgaatgga
    14641 agaaattgcc acaatcgacg ctgctacttt gtttttcagc gacgttgcgt ccattttccc
    14701 cagccagttc agcttctctt tgttctcgga gatatccagc tgtgagacga aattctcata
    14761 accggaaaac atcaccatca ccagcaaacc gccaaccagc gtcatatcca ccagcgacag
    14821 caacacgagg atcaaatctg attccgccat cgagaagata ttcggcagta cgtgaataat
    14881 ctcctggaag aacttcagcg ccagggcaac taacgccagc gaaaggccaa agtacacggg
    14941 ggcaagcagc cagcgagaag catacattgc attttcaaga aaacgttcca tacattcctg
    15001 tcgtgtagtt aaaacgggca acagtatatc gcaattgagt aaacagcacg caacagcaaa
    15061 tcaacatata cgttaaacct ttgtcgcata attctgaggt ttagggatct tcctggttcc
    15121 cttatcaacg catcgagagc gtctaccctt aaaggacgtt gaacctgaag ggagaaaaac
    15181 gatgtctcat ttaaaagacc cgaccacgca gtattacact ggtgaatatc ccaaacagaa
    15241 acaaccgacg ccaggcatcc aggcgaagat gacaccggta ccggattgcg gcgagaaaac
    15301 ctatgttggt agcggtcgcc tgaaagatcg taaagcactg gtgacagggg gcgattccgg
    15361 aataggtcgc gctgccgcca tcgcttacgc gcgtgaaggg gctgacgtgg cgatcagtta
    15421 tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag atcattgaag aatgcggacg
    15481 caaagccgtt ctgctgccag gcgatttaag cgatgagaaa ttcgcccgtt cgctggttca
    15541 cgaagcgcac aaggcgttag gcgggctgga tattatggcg ctggtcgccg ggaaacaggt
    15601 tgccattccg gatattgcag acctcaccag cgaacagttt caaaagacct ttgccattaa
    15661 cgttttcgcg ctgttctggc taacccagga agcgatcccc ctgctaccga aaggtgcaag
    15721 tatcatcacc acttcgtcaa tccaggcata ccagccaagt ccgcatttac tggactatgc
    15781 ggctacgaag gcggcgattc tgaactacag ccgtggcctg gcaaaacagg tcgcggagaa
    15841 aggtattcgg gtgaatattg tcgcgccagg ccctatctgg acggcactgc aaatttccgg
    15901 cggacaaacg caggataaga tcccgcagtt tggtcagaaa acgccgatga agcgtgcggg
    15961 gcaaccggcg gaactggccc ctgtatatgt ttatctggca agtcaggagt cgagctacgt
    16021 caccgcagaa gtgcacggcg tgtgcggcgg cgagcattta ggttaaaaaa atgcccggtt
    16081 gtgaaaagca accgggcatc attgtgaatt cactcttacc tgatgtggcg aaaacgcctt
    16141 atccggccta caaacgcacg aagattcaac atgttgcaat agattttgta ggcctgatga
    16201 gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac
    16261 ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat
    16321 cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat
    16381 tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt
    16441 ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag
    16501 caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca
    16561 gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat
    16621 cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta tcctgcgcgt aatttttgtg
    16681 cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca
    16741 acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa
    16801 atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca
    16861 gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac
    16921 caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg
    16981 cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc
    17041 gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg
    17101 acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa
    17161 aatcagcggc gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct
    17221 cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc
    17281 aagtgactac ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt
    17341 gctgatacat accccagacg gaaaggtccg tctgcatcaa attattaccc actgtgtatc
    17401 tccaggacgc aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag
    17461 tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc
    17521 agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag
    17581 gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc
    17641 gagatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac
    17701 actctcggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgactgtgtg
    17761 gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc
    17821 ggaacgttaa cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc
    17881 tgcgtgctat ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa
    17941 cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc
    18001 aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat
    18061 atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc
    18121 accatggaag tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat
    18181 gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt
    18241 ggcatcgatg tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg
    18301 attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg
    18361 atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta
    18421 ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa
    18481 catccgcaag tagcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc
    18541 tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc
    18601 agtaatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg
    18661 tggggcgggt atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc
    18721 ccacaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat
    18781 gtcgacgatc tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac
    18841 ttttggacaa ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc
    18901 aaggcgtccc ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac
    18961 aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc
    19021 cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt
    19081 tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc
    19141 aggcgcattg attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc
    19201 cgcagcaagt ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa
    19261 aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat
    19321 gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac
    19381 gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa
    19441 ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat
    19501 gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat
    19561 tgccttgtta accgctggct tgttaggcac gctgtttgta gtgattaaaa aaaaatactg
    19621 taacgcctga cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct
    19681 gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact
    19741 cacggctaaa ttgcgatgcg ctttcatagt cgacgcgcat cgctgctgcg ctggccttca
    19801 tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca
    19861 acggcgaggt gctggtgaca gacttaaaat tatgatggaa cgccgatacg ctcatgttgg
    19921 cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca
    19981 gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc
    20041 cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac
    20101 gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt
    20161 ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt
    20221 cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaatcctg
    20281 ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca
    20341 gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc
    20401 cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct
    20461 cgccatacaa caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca
    20521 gtttattaac tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt
    20581 tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct
    20641 ggagaaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac
    20701 aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag
    20761 taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg
    20821 ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggcggcggca
    20881 gcgtgaaaaa aatcggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc
    20941 tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac
    21001 tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg
    21061 gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc
    21121 tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc
    21181 cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca
    21241 agcaggcgtt ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata
    21301 cctacaccct gccgccgcgt caggtggcta acgacgtagt ggacgccttt gtacacaccg
    21361 tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca
    21421 ttttgctgac gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg
    21481 tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg
    21541 taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg
    21601 atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca
    21661 agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg
    21721 atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc
    21781 cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac
    21841 tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc
    21901 gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat
    21961 ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa
    22021 ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca
    22081 gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc
    22141 gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt
    22201 cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct
    22261 gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca
    22321 gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt
    22381 cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt
    22441 gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt
    22501 gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc
    22561 gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt
    22621 cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaagccccgg cgcagattgt
    22681 tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg
    22741 tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat
    22801 tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca
    22861 tgcaaattct cccggtgacg gtaatgttcc gctaccggac ttttcagaaa tcatttattc
    22921 ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc
    22981 atcatgcaga gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac
    23041 ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta
    23101 ccgtacacgc gcagcctcct tcacagaccg tagaacaaac agtccggcag atttatcaga
    23161 actataaatc agatgccagt acaccttatt tcggtgaaac cggagagcgg gcgataactt
    23221 cagcgcgtat tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg
    23281 gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa
    23341 gcgttgcgat aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct
    23401 ttaaagatga taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt
    23461 gggtcattga cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
    23521 atgaaaaaac gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg
    23581 ccgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
    23641 cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg
    23701 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
    23761 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
    23821 gcattcatgt tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac
    23881 agcggcgcga aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
    23941 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca
    24001 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    24061 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact
    24121 ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg cttgagcagg atataaaagc
    24181 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    24241 tgctcaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
    24301 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    24361 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    24421 caggcgtctg acgctgggtc gccatcggcg tatgtttagt caacgccgga cgggtattgc
    24481 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    24541 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgccgggcgg attaacggcc
    24601 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    24661 tcggtacgaa gacgtcttcg ctcttataac caatcttcgc cagcggtttt ttcccggtgt
    24721 aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac
    24781 ggaaacgatg ctttttcagc cacagcgcca aattcaccat atcttcatca cgcgtaccgg
    24841 ggtgcgcaga gatgaaatac gggatcagat actgctcttt acctgcctgt ttcgagtagg
    24901 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgaca
    24961 acggcccttc ttcggtatgt tccggagcaa tcttcagata accgccgacg tgatgggtcg
    25021 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
    25081 tgaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
    25141 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
    25201 tttgttcagc gcgtggcgat ttgcagtgca acatatacat gttggcagtt ggcccaccga
    25261 gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa
    25321 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga
    25381 aagaacagcc gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag
    25441 ccggaatacg ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa
    25501 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    25561 caccgtgttt ttgcatcaat gcgcggacgc agcctgggtt ggtttcgtgg tgcagaatac
    25621 gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca
    25681 cgtaggtttt ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct
    25741 gctttttcgg tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg
    25801 ggattgggtc gatttttcca ggggtatcaa gacgggggga atccacgccg ctccagccag
    25861 gcagcgcctc tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct
    25921 cgcccatcgc cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca
    25981 gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat
    26041 aatgtgcggt acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc
    26101 acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga
    26161 cgttatccgg cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga
    26221 tcatcgaatc catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca
    26281 taaagtcgtc tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgtg
    26341 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    26401 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    26461 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    26521 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    26581 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaagta ttccatactc
    26641 gccctcctcg ggcgagtatg aagattacgg taccggattg actaacagtt gcccaatcga
    26701 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    26761 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    26821 gcctctgtct tccgggaacg gcatcgcacc attcacgtta cggatctgga acatcacgcc
    26881 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    26941 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    27001 acccagactg atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa
    27061 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    27121 cagggtagaa accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct
    27181 cgccgcttcg ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc
    27241 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    27301 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    27361 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    27421 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    27481 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    27541 cacatcataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    27601 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    27661 cagagtagct gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat
    27721 catccgtgcc ggaccgccca tcagcgggcc aggtacctgt agcccggcca ccgtcattga
    27781 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    27841 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    27901 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    27961 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    28021 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    28081 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    28141 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    28201 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    28261 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    28321 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    28381 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    28441 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    28501 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    28561 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    28621 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    28681 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    28741 ccgaacagcg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    28801 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    28861 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    28921 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    28981 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    29041 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg
    29101 aaacaggccc tccccggcat attactcttc gctatcaccg ttgctggcac ggcgaggaga
    29161 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    29221 ttcaccagtg actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg
    29281 aatggtcagc gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg
    29341 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    29401 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    29461 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    29521 agccttacct gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc
    29581 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    29641 cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg
    29701 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccgattg
    29761 gttgctctta cctttcaccg ccactttgaa gctatcaccc gctttataat tcaggcccgg
    29821 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    29881 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    29941 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    30001 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    30061 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    30121 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    30181 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    30241 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    30301 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    30361 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    30421 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    30481 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    30541 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacacgcg cacctgaaac
    30601 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    30661 acgtactgaa ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat
    30721 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt
    30781 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    30841 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    30901 acgagcaggc agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    30961 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    31021 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    31081 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    31141 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    31201 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    31261 aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    31321 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    31381 taattctgat tcctcaactg attcgccagc cttcaaaggg gcaatattgc cggagatact
    31441 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    31501 cgacggcgat aaaggcatca atgtgccttg atatacaacg ctttgctgta gacttgatcg
    31561 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    31621 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    31681 gactataagt atatggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    31741 tgccataagt gcggcttgct catgggtacc cccgagacgg cttacggcgc accggataat
    31801 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    31861 ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    31921 ccccatcacg ctaacggcac ataaacatcc gtttccagtc gctgctccgt caccccttcg
    31981 gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc
    32041 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    32101 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    32161 ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    32221 gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    32281 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    32341 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    32401 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    32461 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    32521 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    32581 gtggtcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    32641 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    32701 gctttctaca gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt
    32761 tgccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcac tgcacagggc
    32821 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    32881 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttaatg
    32941 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    33001 aaaaatgcga attttactga tagaagatga catgctgatt ggcgacggca tcaaaactgg
    33061 ccttagtaaa atgggtttta gtgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    33121 gctttatagc gcaccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    33181 tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt gagccggtac tgatcctgac
    33241 cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    33301 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaggctctga tgcgccgaac
    33361 caacggccag gccagcaacg agctgcgcca cggtaacgtt atgctcgacc ccggcaaacg
    33421 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    33481 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    33541 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    33601 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    33661 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    33721 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    33781 gatgaattgt tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc
    33841 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    33901 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaat
    33961 gatggcgata acggagaaga tattccctat agctatcaac gggaaggttt tgctgacggg
    34021 caactggtcg gtgaagacga tccttggcgt tttgtctgga tgacctcacc tgatggcaaa
    34081 tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    34141 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    34201 ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg tatgcgtgac
    34261 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg accactggtt
    34321 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgaga acgacgcttt
    34381 acctccgatg cagctcacga actacgtagc ccgttaacag cgctgaaagt gcaaaccgaa
    34441 gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa aagcactgct ccaattacat
    34501 tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc gcggctggac
    34561 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg
    34621 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    34681 gcccacagca tcaaacgcac tggacaaccg ctattgctaa gtttgttggt gcgaaacctt
    34741 ctggataacg cagtgcgcta cagtccacag ggcagcgtgg tggacgtcac gctgaatgcc
    34801 gataatttca tcgtgaggga taacggcccc ggtgtgacac cagaggcact ggcgcgaatt
    34861 ggcgaacgct tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg
    34921 attgtccagc gaatcgccaa attgcatggc atgaatgttg aatttgggaa tgcggaacaa
    34981 ggtggatttg aggcgaaggt gagctggtaa gggtaagact tttgctaaat tcgcaaaaga
    35041 ctttgcacat tttgctaatt ttaccgtacc gctctgtgac gtactatagt cgacaaacgt
    35101 ctcaccttga ggttaaaaaa tgagcaacac cctgattatc aacggcgcga aaaaattcgc
    35161 ccactccaat ggtcaactga acgacaccct gaccgaagtc gcggatggca cactgcgcga
    35221 cctcgggcat gatgttcgca tcgttcgcgc cgacagcgac tacgatgtca aagcggaagt
    35281 acaaaacttt ctctgggctg atgtggtgat ctggcagatg ccaggctggt ggatgggcgc
    35341 gccgtggaca gtgaaaaaat acattgatga tgtattcacc gaaggtcacg ggacgctgta
    35401 tgccagcgat ggccgtaccc gcaaagatcc gtcaaaaaaa tacggttccg gcggcctggt
    35461 acagggcaaa aaatatatgc tttctctgac ctggaacgca ccaatggaag ccttcaccga
    35521 aaaagatcaa ttcttccacg gcgttggcgt cgacggtgtg tatctgccgt tccataaagc
    35581 aaaccaattc cttggcatgg aaccgctgcc gacatttatc gctaatgacg tgataaaaat
    35641 gcctgatgtt ccccgctata ctgaagaata tcgcaagcat cttgtggaaa tttttggtta
    35701 actagagctc aggctttaga aggagttaac catgcttacc gtaatcgcag aaatccgtac
    35761 tcgtcctggt caacatcacc gtcaggcggt attggatcag tttgctaaaa tcgttccaac
    35821 cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg gtggattgcg ctgctggcgt
    35881 gagtttccag tctatggcac cggattctat cgtgatgatt gagcagtggg aaagcatcgc
    35941 gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg tacagcgaag ccgtaaaagg
    36001 cgacgtgctg gagatgaata ttcgtattct gcagccaggg atttaatcct gccttgtttg
    36061 cccggccatc ctgaccgggc aatgttcttt cctttaaacc tcaatctccg ccatgtcgcc
    36121 tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca gcatatccat
    36181 catcgcgtca gtacgctgat cgtcttcatc atcgatagtc aactgcacca gacggcgagt
    36241 gttcggatca agcgtggttt cacgcaattg catcgggttc atttccccca gacctttaaa
    36301 acgctggacg ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc cctctttctc
    36361 ttcttccgtc agcgcgtaat aaacctcttt cccgagatca atacggtaga gcggtggcag
    36421 tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga acaaagcgca
    36481 gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc gcgaggatac agattttgcc
    36541 ataacgaagc tggctcagat cgtcgctgtt aggatcgata ccgatcgcta ccgaaatatc
    36601 atgcacttcc tgcgaggcca gcacttcgtc ggaagaaact tcccaggtgt taaggatctt
    36661 acctttcagc ggcatgatcg cctgatattc acgatcgcgc gcctgcttgg cagatccgcc
    36721 tgcggagtca ccttccacga ggaacagttc ggtacggtta aggtcctgcg cggtacaatc
    36781 agccagtttg ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca cttttttagc
    36841 cgcacgcata cgacgctggg cgctggaaat cgccatctct gccagcaact ccgccgcctg
    36901 aacgttctgg ttcagccaca ggataaaggc atctttcacc acgccagaaa cgaatgccgc
    36961 gcattgacgc gaagagagac gctctttcgt ctgcccggca aactgcggat cctgcatttt
    37021 caccgacaga acataggcgc agcgatccca gatatcttcc gccgacagct tcacgccgcg
    37081 cggcagaatg ttgcgatatt cacagaactc acgcatcgcg tccaacaggc cctgacgcag
    37141 gccattaaca tgggtaccgc cctgcatcgt tgggatcagg ttgacgtagc tttcggtcag
    37201 cagttcaccg ccttccggca gccacagtag cgcccagtcc acagcttcag tatcgccagc
    37261 gaaattaccg ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg cttccgccag
    37321 gtaatcattc agaccgtcct gatagcacca gcgctgttcg gtgttgttga tctcatcttt
    37381 aaaggtaatc tcaacgccgg ggcacagtac cgctttggct ttcagcacat gcgtcaggcg
    37441 tgaaacagaa aatcgcgggc tgtcaaagaa ggtttcatcc ggccagaagt gcacgctggt
    37501 accggtattg cgtttaccgc aagtgccgac aacctgtaaa tcctgcacct tttcgccatt
    37561 ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg ttaacttcaa cgcgcttcga
    37621 cagggcgtta accaccgaaa tccccacgcc atgcaggccg ccagagaact ggtaattttt
    37681 gttagagaat ttaccgcctg catgcagacg gcaaagaatc aattcaaccg ccgggacacc
    37741 ctcttccggg tgaatatcca ccggcatccc gcgcccatcg tcaataactt ctaacgactg
    37801 gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc gccagtgctt catcgacact
    37861 gttatcaata acttcttgcc ccaaatggtt agggcgagtg gtatcggtat acatccccgg
    37921 acggcggcga accggctcaa gcccggtgag tacctcaatg gcatcagcgt tataagtttg
    37981 cgtcatggtt taagttagca attcgagttg atcgtcagag atggtgcaga ccaagaaaat
    38041 cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga
    38101 cagtctggcg gcaggaagcg tagtacgcca ccgcctggcg gtaatccagc acttcatctc
    38161 ccgtctgttg cagcagccag atcaaatccg gcgcttccag cgggtcaatc tgcatgactt
    38221 tgagatcgta aatatggcgt gactctagca catattgctg cccggtgtat gggttctcgt
    38281 tctgaccgag atagtccgtc agcagttcaa acgggcgcac cgccgggttt accaccactg
    38341 cgggcagcat aaaacattgt gacaaccagg tggcgtaata tccccccagt gacgaaccga
    38401 caatacccag cgaatcgccg ccatgttcca ggacaatgga ttccagcagt tctgccgcgt
    38461 cggaaggata cggcggcaac tgcggaatga tcatctcaac gtcagggtga tgttccgcca
    38521 gccagttttt taacaagctc gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat
    38581 aaagaagcgt agacatcagt agccttctga agcggtatca ggttggaaac gtgtgtccgc
    38641 caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt aactcgagag tacgccagcc
    38701 gggcgcgata gtatccagcg taaagttgga acagtgcggc ttaaactgca cacaggtcga
    38761 cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc agctcctgat gaatatgacc
    38821 gcacagcaag tatttgacgt gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt
    38881 acgcagactg tgttgatcga gccaactaca acccgcaggt agcggatgat gatgcagcag
    38941 cagcaacgta tggcgttctg gcgcatcggc cagtttacgt tccagccact caagctgaaa
    39001 ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca ggatttgcca
    39061 ttgctcacca ataaacaccc gcttcgccgg ggagataccc gcatcctgta acgcgctgta
    39121 catcgcgggc tggaaatcgt ggttgcccgg cagccagacg cagggcgcac gaaaacttgc
    39181 gatgccttca gcgaaatgct gataggccgc agaggattga tcctgcgcta aatcacctgt
    39241 cgcgacaatc aggtcgaatt cgtgctgatg tggacgaatc gcctccagca ccgcctggta
    39301 gctttcccag gtgtttaccc ctaacagagc ttcgtgcttt tgtgcaaaca ggtgagtgtc
    39361 ggtaatttgt aaaatcctga ctctggcctc accagccaga ggaagggtta acaggctttc
    39421 caaatggtgt ccttaggttt cacgatgcta gtaaaccgga atcgccatcg ctccatgtgc
    39481 taaacagtat cgcaaccagt ccgctaaaaa ctgattaatt tgatgctttt cgtcgcgttg
    39541 atgcaacttt ttattaggat aatcataccg cgctttgaag cgaaaaatct gctggcttga
    39601 acacacttca gccaccatcg cgtcatgata cagacgcacc gtcattgacg gaaggctcca
    39661 gtaactgatc gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc gggtcgattc
    39721 cacaatcgtc agccgatatt gtgcgtttgc cacctgatag cttacagttt cgccgggtgc
    39781 gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat
    39841 ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac tcatttttta acgcttgatg
    39901 atgcagctgc agccattgca aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc
    39961 tacccactgg tatgcctgtt cccggcttac cacatgaacg cgaatatctt cgttttcatc
    40021 agccagacca tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca taattgacga
    40081 acgctcactg gtgccccccg ggcttgccag gaaacttaac accggtttgg tccgtttgac
    40141 tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac tttcaccctc
    40201 ttctatcatc ccggcaacca tctccagtag ccagggggtt tcactggtgt cgtatgcggc
    40261 aatccgaatc tgctcaatca gcacaacttc atcgcgcact gggtcaaagg gtagcaagac
    40321 tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca tgactcattt gcccgttgaa
    40381 tagacgatga cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg
    40441 tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat
    40501 tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg
    40561 tttgagcagt catgtgttaa attgaggcac attaacgccc tatggcacgt aacgccaacc
    40621 ttttgcggta gcggcttctg ctagaatccg caataatttt acagtttgat cgcgctaaat
    40681 actgcttcac cacaaggaat gcaaatgaag aaattgctcc ccattcttat cggcctgagc
    40741 ctttctgggt tcagttcgtt gagccaggcc gagaacctga tgcaagttta tcagcaagca
    40801 cgccttagta acccggaatt gcgtaagtct gccgccgatc gtgatgctgc ctttgaaaaa
    40861 attaatgaag cgcgcagtcc attactgcca cagctaggtt taggtgcaga ttacacctat
    40921 agcaacggct accgcgacgc gaacggcatc aactctaacg cgaccagtgc gtccctgcag
    40981 ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca ggaaaaagca
    41041 gcagggattc aggacgtcac atatcagacc gatcagcaaa ccttgatcct caacaccgcg
    41101 accgcttatt tcaacgtgtt gaatgctatt gacgttcttt cctatacaca ggcacaaaaa
    41161 gaagcgatct accgtcaatt agatcaaacc acccaacgtt ttaacgtggg cctggtagcg
    41221 atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa cgaagtgacc
    41281 gcacgtaata accttgataa cgcggtagag cagctgcgcc agatcaccgg taactactat
    41341 ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca gccggttaac
    41401 gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg cttgagccag
    41461 gacctggcgc gcgagcaaat tcgccaggcg caggatggtc acttaccgac gctggattta
    41521 acggcttcta ccgggatttc tgacacctct tatagcggtt cgaaaacccg tggtgccgct
    41581 ggtacccagt atgacgacag caatatgggc cagaacaaag tgggcctgag cttctcgctg
    41641 ccgatttatc agggcggaat ggttaactcg caggtgaaac aggcccagta caactttgtt
    41701 ggtgccagcg agcaactgga aagcgcgcat cgtagcgtcg tgcaaaccgt acgttcctcc
    41761 ttcaacaaca ttaatgcatc tatcagtagc attaacgcct acaaacaagc cgtagtttcc
    41821 gctcaaagct cattagacgc gatggaagcg ggctactcgg tcggtacgcg taccattgtt
    41881 gatgtgttgg atgcaaccac cacgctgtac aacgctaagc aagagctggc aaatgcgcgt
    41941 tataactacc tgattaacca gttgaatatt aagtcagctc tgggtacgtt gaacgagcag
    42001 gatctgctgg cactgaacaa tgcgctgagc aaaccggttt ccactaatcc ggaaaacgtt
    42061 gcaccgcaaa cgccggaaca gaatgctatt gctgatggtt atgcgcctga tagcccggca
    42121 ccagtcgttc agcaaacatc cgcacgcact accaccagta acggtcataa ccctttccgt
    42181 aactgacgac gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt aaagatacgg
    42241 gttatctgcc gcattcttcc cccttctcgc ttcaatttcg accagccatc ctctattctg
    42301 atgggtattt accactggtc ccggaagaca aaaatgaaac ggacaaaatc catacgccac
    42361 gcatcgttcc gcaaaaactg gagcgcacgc catctgacac cagtcgctct cgcggttgcc
    42421 actgttttta tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct ctatcaaaat
    42481 gctgacgact gttcagctgc aaacccaggc aaaagcgccg aatgtaccac cgcgtacaac
    42541 aatgcgctga aagaagccga acgtactgcg ccgaaatacg ccacccgtga agactgtgtt
    42601 gctgaatttg gtgaaggtca gtgccagcag gcaccagccc aggctggcat ggcaccagaa
    42661 aaccaggcgc aggcccagca atccagcggg agtttctgga tgccgctgat ggccggttac
    42721 atgatggggc gtctgatggg cggcggcgcg ggatttgcac agcagccgct gttctcctcg
    42781 aaaaacccag ccagcccggc ttacggtaaa tataccgacg cgacgggtaa aaactatggc
    42841 gcagcccagc caggccgcac catgaccgta ccgaagacag ccatggcacc aaaaccggcg
    42901 accactacta ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca aagcactatg
    42961 cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg gctgataccg atggaaagag
    43021 tcagtattac cgagcgcccg gactggcgcg aaaaagccca cgaatacggt ttcaattttc
    43081 acaccatgta cggcgagccg tactggtgtg aagatgctta ctacaagttg accctcgccc
    43141 aggttgaaaa gctggaagaa gtcaccgccg aactgcacca gatgtgcctg aaagtggtgg
    43201 aaaaagtgat cgccagcgat gagctgatga ccaaattccg cattccaaaa cgcacctgga
    43261 gttttgtgcg tcagtcatgg ctgactcacc agccatcgct ttattcgcgt cttgatctgg
    43321 catgggatgg cactggcgaa cctaaactgc tggaaaataa cgccgataca ccaacgtcac
    43381 tatacgaggc ggcgttcttt cagtggatct ggctggaaga tcagcttaac gccggtaact
    43441 tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa gctaatcgat cgctttgtcg
    43501 agttgcgtga acagtatggc ttccagttgc tgcatctcac ctgctgtcgc gacacggtgg
    43561 aagatcgcgg aaccattcag tatttgcagg actgcgcgac ggaagctgaa atcgctaccg
    43621 agttcctcta cattgacgat atcgggttag gtgaaaaagg tcagttcacg gatttacagg
    43681 atcaggtaat ttccaacctg ttcaagctgt atccatggga atttatgttg cgtgagatgt
    43741 tctccaccaa gctggaggat gcaggcgtac gctggctgga accggcgtgg aagagcatta
    43801 tctccaacaa agcgcttcta ccgctactat gggagatgtt cccgaatcac ccgaacctgc
    43861 tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg gttaaaccga
    43921 tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc attgaagcag
    43981 cggaaggtcc gtatggcgaa gaagggatga ttgttcagca attccacccg ttaccgaaat
    44041 tcggcgacag ctatatgctg attggtagct ggctggtgaa cgatcaacct gccggaattg
    44101 gcattcgtga agaccgtgcg ttgatcaccc aggatatgtc tcggttttat ccacatattt
    44161 ttattgagta atcaatgatg ccggatggca ttcgccatcc ggtaattgtt aacctatctg
    44221 caccgacagc atactcaggc tgcccatttc tataccatcg accggaatgg taattggctc
    44281 ctgcccatcc cacgcaccta agacatacaa caacggcaga tagtgctccg gcgttgggtt
    44341 cgataacgcg ccaccttcat ggtcaaggta attcaccaga ggatgctgct ctaccgcccc
    44401 ttgccacgtc agattcgctt tcacatactc attaaacgac atcgcccaag gatatggtga
    44461 actatcaccg tgccacttca ctgtacgcag gttatgcacc acgttgccac tggcgaccaa
    44521 cattattcct tcatctcgca gcgctgccag tttgcgcccc atttcgaaat gccaggcggc
    44581 aggtttgcta ctgtcgatac tcaactgcac catcgggata tcggcgtcag gatacatctt
    44641 aatcagcacg ccccaggaac cgtggtcaaa gccccaggct tctttatcca gcgtcaccgg
    44701 gaccgacgct aacagctcaa ccagatgctg tgccagcaca ggcgaacccg gagcaggata
    44761 atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg gcggcgtctc
    44821 catcgcggtc actcctgttc cacgggtaaa ccagtgagcc gaaaccacca caatcgcttg
    44881 cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg gtatacaaat tatcttccag
    44941 cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg aagacatgat
    45001 gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt cggcggagta
    45061 agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc tgaaggtgaa
    45121 gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg cgggggcagc
    45181 cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc gcttactggc
    45241 gttttcgcta ggatttgcgg cggggatcat gttgctcatc tcattaatgg aaatgcttcc
    45301 tgccgcacta gcagctgaag gaatgtcgcc tgtgttgggt tatggaatgt ttatcttcgg
    45361 tctgcttggc tattttggcc tggaccgcat gttgccacat gcgcatccgc aggatttaat
    45421 gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc tgctcactct
    45481 cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg cgagcagcaa
    45541 cctggagctg ggatttggca tcgcactggc cgtcgcgttg cacaatatcc ctgaaggtct
    45601 ggcagtggca ggcccggttt atgcggcaac ggggtctaaa cgcaccgcga ttctgtgggc
    45661 ggggatttct ggactagcag aaattcttgg tggtgtgctg gcgtggttaa tcctcggtag
    45721 catgatttcc ccggtggtca tggcggcaat catggcggcg gttgcaggaa ttatggtggc
    45781 gctctcggtt gatgaattaa tgccgctcgc caaagagatt gaccctaata ataaccccag
    45841 ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc tacaaacggc
    45901 gggaattggt taaaaacaac atcagaagtt gtccagcaca tggacaactt ctgccagctt
    45961 cacgaaagcc ttattctacc ttttcggctg tctcttctct cgtactgttt aagtatttgt
    46021 tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc ccgacaataa cgtttcaaat
    46081 gcaggaaata acccaccaca accagagcaa tcaatacaat ccagtaccag gcgataaaca
    46141 tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt cacatatcct
    46201 ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt ttgatcataa
    46261 tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat
    46321 cttgcatagc agggcaaaat aaaaaccgaa gccagctaca acttcggttt cttgattaag
    46381 gcagtaatta agcagcggtt ttcagctggc tttacgctca tgtgcctgac ggtatgccac
    46441 caggtcttca atagtcacga gcgccatatt gtgtttattg gcaaactcaa tgcactctgg
    46501 tgcacgcgcc atcgtgccat cgtcattagt cagctcacac agcacaccag ccggtttaaa
    46561 gcctgccagc gtcatcaaat caatagtcgc ttcagtatga ccgccacgcg tcagtacacc
    46621 acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag aaggttttgc
    46681 gccatcggca atcgctgcgc gaacggtcgt aatacgatca gcggcagaaa caccggtagt
    46741 cacaccttca gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc tggtgttatt
    46801 ttctaccatc attggcagat caagttgttt acggcgatct tcagtgatgc acaggcaaac
    46861 aataccgcta ccgtggcgaa tggtcagcgc catctgctca acggtcatgg tttctgccgg
    46921 gaagatcata tcgccttcgt tttcacggtc ttcatcatca agcaccatta caccgcgtcc
    46981 ttcacgcagc gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa aagaggaaag
    47041 tagcgtctga ttcatggtaa aaaaacctca ctaaaattat ggttaccaga atcagggcag
    47101 tcttaggagt ggcggcatat agccaaaata acgtgagcgg gtccatgccc gacagaatcg
    47161 ttactctctc ccatccggac tctaaccgtc ggccccggaa ttacaccgga tctgctgtcc
    47221 tttgagttcg cacccaaagc gctcgcgggc tttcaactga gttgatttac cgccggtggg
    47281 gaatttcgcc ccgccctgag aataagcgga ttcactataa cgctaatgat tagcggcagc
    47341 aacgcatagc ttcacataat tctggtttat gacttaccct tatcgcacta caatggtact
    47401 caacacctat cagtacaggg aaactacaat gattgacccg aaaaaaattg agcaaatcgc
    47461 tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag ttcggggaag atgtggagaa
    47521 aaaaatccgc caaaccctac aagctcagct gacgcgcctc gaccttgtaa gccgtgaaga
    47581 gttcgacgtc caaacgcaag tcctgttacg gactcgtgag aaactggcgc ggcttgaaca
    47641 aagaatgagc gaactggaaa atcgcagtac tgagattaaa aagcagccag atccagagac
    47701 tctcccccca acgctgtaaa catttgtctt taatgcactg taaattatga attaatcttc
    47761 taatttacag tgctttctat taccttatcg cttaattcca ttcagaatat catcacaaaa
    47821 atgaatgagt gcaattgaaa atataattgt tatgtatttt ttaagtttta cttaatccat
    47881 atttagataa actaatttca taaaacccga aaagtaaaat ataatatttg aatgattagc
    47941 aatccttaaa taacagttac taatcattca ttttctactt atatttatat aaaggaatat
    48001 tatgtctgct tttaaaaagt cacttcttgt tgctggcgta gcaatgattc tttccaataa
    48061 cgtatttgct gacgaaggtc acggcattgt taaatttaaa ggtgaagtta tctctgctcc
    48121 atgttcaatt aaaccaggtg acgaagactt aactgtaaat ctgggtgaag tagcagatac
    48181 cgttctgaaa agcgaccaga aatctttggc tgagcctttc actattcacc tgcaagactg
    48241 catgttgagc cagggtggaa ccacttattc aaaagcgaag gttactttca ccacagcgaa
    48301 tactatgacg ggtcagactg acctgctgaa aaacaccaaa gaaactgaaa tcggtggtgc
    48361 cacaggcgtt ggcgtacgta ttctggatag ccagagcggc gaagtgactt taggtactcc
    48421 ggtagtaatt acattcaaca atactaacag ctatcaggaa ctgaacttca aagctcgtat
    48481 ggaatccccg ggcaaagatg ctaccccggg caacgtttac gctcaggctg actataagat
    48541 tgcttacgag taatcacatt aggccgggtt cgcccggcca catcattaac ggattaatga
    48601 taagtggaac agatgtataa aaaattaaaa ttaaccacaa taagcgaatt gattaaaaat
    48661 atttattgtt cattatccgt tctcatcatt ggttgtgcgt cagcttatgc cgttgaattc
    48721 aacaaagatt taatcgaagc cgaagatcgt gaaaacgtta acctttccca atttgaaact
    48781 gatggccaat tacccgtagg taaatactca ttaaacgctc ttattaataa taaaaggacg
    48841 ccaatccatc tggacctgca atgggtatta attgataacc aaactgcagt ttgtctgact
    48901 ccagagcaat taacattatt aggctttact gatgagatta ttgaagaggc tcagcaaaac
    48961 ctgatcgatg gttgttaccc tatcgaaaaa gaaaaacaaa ttacaactta tctcgataaa
    49021 gggaaaatgc aattatccat atctgcacct caggcatggt taaaatacaa agatgcaaac
    49081 tggacgcctc ctgaactttg ggatcatggt attgctgggg catttcttga ctacaattta
    49141 tatgcctctc attatgcacc acatcagggc gataattcac aaaatataag ttcctatggg
    49201 caggctgggg ttaatcttgg ggcctggcgc ctgcgtactg attaccagta cgatcagtca
    49261 tttaacaatg gcaaaagcca ggcgaacaac ctggattttc cgcgtattta tttgtttcgc
    49321 ccaatcccag caattaatgc aaaactaact ataggtcaat acgatactga atcctctatt
    49381 ttcgactctt tccatttttc tggcgtttcg ttgaaaagcg atgagaatat gttgccgcct
    49441 gatctacgtg gttacgcacc gcaaatcacg ggtgttgcac aaacgaatgc aaaggtcact
    49501 gtctcacaga acaaccgtat tatttatcaa gaaaatgttc ctcctggccc atttgctatt
    49561 actaatttat tcaatacatt acaggggcaa ctggacgtca aggttgaaga agaggacggg
    49621 ccagtctata tatcaatgac a
//
LOCUS       DAJTXQ010000013        46284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000013 DAJTXQ010000000
VERSION     DAJTXQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.013"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..472)
                     /locus_tag="OO515_000724"
     CDS             complement(<1..472)
                     /locus_tag="OO515_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6574738.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVT"
     gene            543..1256
                     /gene="bdcA"
                     /locus_tag="OO515_000725"
     CDS             543..1256
                     /gene="bdcA"
                     /locus_tag="OO515_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6574739.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1387..1782
                     /locus_tag="OO515_000726"
     CDS             1387..1782
                     /locus_tag="OO515_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6574740.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2063..2197
                     /gene="pyrL"
                     /locus_tag="OO515_000727"
     CDS             2063..2197
                     /gene="pyrL"
                     /locus_tag="OO515_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6574741.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2201..3136
                     /gene="pyrB"
                     /locus_tag="OO515_000728"
     CDS             2201..3136
                     /gene="pyrB"
                     /locus_tag="OO515_000728"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6574742.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3149..3610
                     /gene="pyrI"
                     /locus_tag="OO515_000729"
     CDS             3149..3610
                     /gene="pyrI"
                     /locus_tag="OO515_000729"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6574743.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            3683..4069
                     /gene="ridA"
                     /locus_tag="OO515_000730"
     CDS             3683..4069
                     /gene="ridA"
                     /locus_tag="OO515_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6574744.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4275..6971)
                     /gene="mgtA"
                     /locus_tag="OO515_000731"
     CDS             complement(4275..6971)
                     /gene="mgtA"
                     /locus_tag="OO515_000731"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6574745.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7112..7165)
                     /gene="mgtL"
                     /locus_tag="OO515_000732"
     CDS             complement(7112..7165)
                     /gene="mgtL"
                     /locus_tag="OO515_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6574746.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7350..8297
                     /gene="treR"
                     /locus_tag="OO515_000733"
     CDS             7350..8297
                     /gene="treR"
                     /locus_tag="OO515_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6574747.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8416..9837
                     /gene="treB"
                     /locus_tag="OO515_000734"
     CDS             8416..9837
                     /gene="treB"
                     /locus_tag="OO515_000734"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6574748.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            9887..11542
                     /gene="treC"
                     /locus_tag="OO515_000735"
     CDS             9887..11542
                     /gene="treC"
                     /locus_tag="OO515_000735"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6574749.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            11936..14074
                     /gene="nrdD"
                     /locus_tag="OO515_000736"
     CDS             11936..14074
                     /gene="nrdD"
                     /locus_tag="OO515_000736"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6574750.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14232..14696
                     /gene="nrdG"
                     /locus_tag="OO515_000737"
     CDS             14232..14696
                     /gene="nrdG"
                     /locus_tag="OO515_000737"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6574751.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(14741..15127)
                     /gene="cybC"
                     /locus_tag="OO515_000738"
     CDS             complement(14741..15127)
                     /gene="cybC"
                     /locus_tag="OO515_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6574752.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15311..16663)
                     /gene="pmbA"
                     /locus_tag="OO515_000739"
     CDS             complement(15311..16663)
                     /gene="pmbA"
                     /locus_tag="OO515_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6574753.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            16757..17308
                     /locus_tag="OO515_000740"
     CDS             16757..17308
                     /locus_tag="OO515_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6574754.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17391..18764)
                     /gene="mpl"
                     /locus_tag="OO515_000741"
     CDS             complement(17391..18764)
                     /gene="mpl"
                     /locus_tag="OO515_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6574755.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            18940..19938
                     /gene="fbp"
                     /locus_tag="OO515_000742"
     CDS             18940..19938
                     /gene="fbp"
                     /locus_tag="OO515_000742"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6574756.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(19971..20966)
                     /gene="yjfF"
                     /locus_tag="OO515_000743"
     CDS             complement(19971..20966)
                     /gene="yjfF"
                     /locus_tag="OO515_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6574757.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(20953..21975)
                     /locus_tag="OO515_000744"
     CDS             complement(20953..21975)
                     /locus_tag="OO515_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6574758.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(21989..23491)
                     /locus_tag="OO515_000745"
     CDS             complement(21989..23491)
                     /locus_tag="OO515_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6574759.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(23631..24587)
                     /gene="ytfQ"
                     /locus_tag="OO515_000746"
     CDS             complement(23631..24587)
                     /gene="ytfQ"
                     /locus_tag="OO515_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6574760.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24897..25427
                     /gene="ppa"
                     /locus_tag="OO515_000747"
     CDS             24897..25427
                     /gene="ppa"
                     /locus_tag="OO515_000747"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6574761.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(25806..26147)
                     /locus_tag="OO515_000748"
     CDS             complement(25806..26147)
                     /locus_tag="OO515_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6574762.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26150..29929)
                     /gene="tamB"
                     /locus_tag="OO515_000749"
     CDS             complement(26150..29929)
                     /gene="tamB"
                     /locus_tag="OO515_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6574763.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(29926..31659)
                     /gene="tamA"
                     /locus_tag="OO515_000750"
     CDS             complement(29926..31659)
                     /gene="tamA"
                     /locus_tag="OO515_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6574764.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            31865..32503
                     /gene="msrA"
                     /locus_tag="OO515_000751"
     CDS             31865..32503
                     /gene="msrA"
                     /locus_tag="OO515_000751"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6574765.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            32826..34169
                     /locus_tag="OO515_000752"
     CDS             32826..34169
                     /locus_tag="OO515_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6574766.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34249..34455)
                     /locus_tag="OO515_000753"
     CDS             complement(34249..34455)
                     /locus_tag="OO515_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6574767.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            34780..35334
                     /locus_tag="OO515_000754"
     CDS             34780..35334
                     /locus_tag="OO515_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6574768.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35424..36335)
                     /locus_tag="OO515_000755"
     CDS             complement(35424..36335)
                     /locus_tag="OO515_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574769.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36547..37287)
                     /gene="cysQ"
                     /locus_tag="OO515_000756"
     CDS             complement(36547..37287)
                     /gene="cysQ"
                     /locus_tag="OO515_000756"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6574770.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37477..39420
                     /gene="cpdB"
                     /locus_tag="OO515_000757"
     CDS             37477..39420
                     /gene="cpdB"
                     /locus_tag="OO515_000757"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6574771.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39538..39918)
                     /locus_tag="OO515_000758"
     CDS             complement(39538..39918)
                     /locus_tag="OO515_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6574772.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40007..40867
                     /gene="qorB"
                     /locus_tag="OO515_000759"
     CDS             40007..40867
                     /gene="qorB"
                     /locus_tag="OO515_000759"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6574773.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            40975..41940
                     /locus_tag="OO515_000760"
     CDS             40975..41940
                     /locus_tag="OO515_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6574774.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42048..42710
                     /gene="ytfE"
                     /locus_tag="OO515_000761"
     CDS             42048..42710
                     /gene="ytfE"
                     /locus_tag="OO515_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6574775.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(42755..44167)
                     /gene="cycA"
                     /locus_tag="OO515_000762"
                     /gene_synonym="dagA"
     CDS             complement(42755..44167)
                     /gene="cycA"
                     /locus_tag="OO515_000762"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6574776.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44476..45096)
                     /gene="fklB"
                     /locus_tag="OO515_000763"
     CDS             complement(44476..45096)
                     /gene="fklB"
                     /locus_tag="OO515_000763"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6574777.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45315..45953
                     /gene="ytfB"
                     /locus_tag="OO515_000764"
     CDS             45315..45953
                     /gene="ytfB"
                     /locus_tag="OO515_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6574778.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(45937..>46284)
                     /locus_tag="OO515_000765"
     CDS             complement(45937..>46284)
                     /locus_tag="OO515_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6574779.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT        11363 a        12308 c        11549 g        11064 t
ORIGIN      
        1 cagtcacaca ttgtgcgctt tgtggatgcc gcctggcgat atagtcataa atggccgttt
       61 ctgcggcgtg ataatattga acggcaatat cacgcgcttg tggatcatga ctatgaatgc
      121 cttcaagaac catacagcca gcgcagccgc cgttttggct atatctgcgc gccgcttctt
      181 ttaatacctc agccaggcac tcccctaccg gacgatcatc acgaagaata tcggcaagcg
      241 gaatagcttc cgtaccgaca tattcattga gtacacggct aaataaccca gccttactgc
      301 caaaagccgc gtagaggctc ggggggttaa taccaagata atcagtaact tcagcaacac
      361 tgacagcatc gaaacctttt tggtgaaaaa gcacttttgc cgcagagact gcctgctcag
      421 gagcgaaacg tctgggacga cctggaacgc gagattgttt tttagtgacc attacaaaac
      481 ttgttgacag aaagttaaaa cagttttgta atgcatgtta cataataaat caaggagtcc
      541 ttatgggcgc ttttacaggt aagacagttc tcatcctcgg tggcagtcgt ggtatcggtg
      601 ccgctatcgt acgtcgtttc gtcaccgatg gggccaatgt acgattcacc tatgcggggt
      661 cgaaagatgc cgctgaacac ctggcacaaa agactggagc gacagcagta ttcacagata
      721 gtgctgacag agacgctgtc attgatgtcg ttcgtaagag cggcgcattg gatatcctgg
      781 tggtaaatgc aggtattggc gtctttggcg atgccctgga attgaatgcc gacgatattg
      841 atcgcctttt caaaatcaat attcatgctc cttatcatgc ctctgttgaa gccgcccggc
      901 agatgcccga aggcgggcgc atcttaatca tcggctccgt gaatggcgat cgtatgcctg
      961 ttgcaggcat ggctgcttat gccgccagta aatctgccct gcaaggcatg gcgcgcgagc
     1021 tggcccgtga tttcggaccg cgtgggatca ccatcaacgt cgtccagcca gggccaattg
     1081 ataccgacgc taatcccgcc aacgggccaa tgcgcgatat gttgcatagt tttatggcta
     1141 tcaaaagaca tgggcaaccg gaagaggtcg ctggtatggt cgcatggtta gcagggccag
     1201 aagccagttt tgttaccggc gcgatgcata ccattgatgg cgcgtttggc gcataaccga
     1261 ctacgctcaa ttaagcccag ccatttccca tgatgtctgg gttttgttta ctcacgtcgt
     1321 ccgctaaaag cggctcctgg taaatataaa tcttctgttc atgtcgtaaa tcttgaggat
     1381 aaccaaatgg tagaaagaac cgctgttttc cctgcaggcc gacattcact gtatgctgaa
     1441 catcgttatt ctgcggctat tcgttccggc gatttgctgt ttgtttccgg gcaagtagga
     1501 agtcgagagg acggaacacc agaacccgat tttcagcaac aagtcagact ggcatttgat
     1561 aatttgcatg cgaccctggc tgctgcggga tgcacttttg acgatatcat tgatgttacg
     1621 agcttccata ccgatccaga aaaccaattt gaagacatca tgacggtgaa aaatgaaata
     1681 tttagcgccc caccttatcc aaactggacg gcggtaggtg ttacatggct ggcaggcttt
     1741 gattttgaaa ttaaagtgat agcgcgcatc cctgagcagt aagcaatagt gttagccgtt
     1801 cgttttcaca ctccgcccta taagtcggat gaatggaata aaatgcatat ctgattgcgt
     1861 gaaagtgaaa aaggaaaaag caggaaatgt ctgcaattat tgataccgaa cgaccgttcc
     1921 cctgcagaat cacaacaaat aaaaatgcat gtattttgac ttataattca aataaaccgt
     1981 ttgcgctgac aaaatattgc atcaaatgct tgcaccgctt ctgacgatga gtataatgcc
     2041 ggacaatttg ccgggaggat gtatggttca gtgtgttcga cattttgtct taccgcgtct
     2101 gaaaaaagac gctggcctgc cgtttttctt cccgttgatc acccattccc agcccctcaa
     2161 tcgaggggct tttttttgcc caggcgtcag gagataaaag atggctaatc cgctatatca
     2221 gaaacatatc atttccataa acgaccttag tcgcgatgac cttaatctgg tgctggcgac
     2281 agcggcgaaa ctgaaagcaa acccgcaacc agagctgttg aagcacaaag tcattgccag
     2341 ctgtttcttc gaagcctcta cccgtacccg cctctctttc gaaacttcca tgcaccgcct
     2401 gggtgccagc gtggtgggct tctccgacag cgccaataca tcactgggta aaaagggcga
     2461 aacgctggcc gataccattt cggttatcag cacttacgtc gatgcgatag tgatgcgtca
     2521 tccgcaggaa ggtgcggcgc gcctggccac cgagttttcc ggcaatgtac cggtactgaa
     2581 tgccggtgat ggctccaacc aacatccgac gcaaaccttg ctggacttat tcactattca
     2641 ggaaacccag gggcgtctgg acaatctcca cgtcgcaatg gttggtgacc tgaaatatgg
     2701 ccgcaccgtt cactccctga ctcaggcgtt agcgaagttc gacggcaacc gtttttactt
     2761 catcgcgccg gacgcgctgg caatgccgca atacattctg gatatgcttg atgaaaaagg
     2821 gatcgcctgg agtctgcaca gctctattga agaagtgatg gcggaagtag acatcctgta
     2881 catgacccgc gtgcaaaaag agcgtctgga cccgtccgag tacgccaacg tgaaagcgca
     2941 gtttgttctt cgcgccagcg atctccacaa cgccaaagcc aatatgaaag tgctgcatcc
     3001 gctgccgcgt gttgatgaga ttgcgacgga tgttgataaa acgccacacg cctggtactt
     3061 ccagcaggca ggcaacggga ttttcgctcg ccaggcgtta ctggcactgg ttctgaatcg
     3121 cgatctggta ctgtaagggg aaatagagat gacacacgat aataaattgc aggttgaagc
     3181 tattaaacgc ggcacggtaa ttgaccatat ccccgcccag atcggtttta agctgttgag
     3241 tctgttcaag ctgaccgaaa cggatcagcg catcaccatt ggtctgaacc tgccttctgg
     3301 cgagatgggc cgcaaagatc tgatcaaaat cgaaaatacc tttttgagtg aagatcaggt
     3361 agatcaactg gcattgtatg cgccgcaagc cacggttaac cgtatcgaca actatgaagt
     3421 ggtgggtaaa tcacgcccca gtctgccgga gcgcatcgac aatgtgctgg tctgcccgaa
     3481 cagcaactgt atcagccatg ccgaaccggt ttcttccagc tttgccgtgc gaaaacgcgc
     3541 caatgacatc gcgctcaaat gcaaatactg tgaaaaagag ttttcccata atgtggtgct
     3601 ggccaattaa ttgcggttgg taataaaagt ctggctccct ataatgagcc agacttttta
     3661 ccgctgtaat aaaggagaaa tcatgagcaa aactatcgcg acggaaaatg caccggcagc
     3721 tatcggtcct tacgtacagg gcgttgatct gggcaatatg atcatcacct ccggtcagat
     3781 cccggtaaat ccgaaaacgg gcgaagtacc ggcagacgtc gctgcacagg cacgtcagtc
     3841 gctggataac gtaaaagcga tcgtcgaagc cgctggcctg aaagtgggcg acatcgttaa
     3901 aaccaccgtg tttgtgaaag atctgaacga cttcgcaacc gttaatgcta cctacgaagc
     3961 cttcttcacc gaacacaatg ccaccttccc ggcacgttct tgcgttgaag ttgcccgtct
     4021 gccgaaagac gtgaaaattg agatcgaagc gatcgctgtt cgtcgctaat cttgatggaa
     4081 atccgggcta tcatgcccgg attaagtctg atgacaaaca caaaatcgcc tgatgcgcta
     4141 cgcttatcag gcctacgtga ttcctgcaat ttattgaatt tgttggccgg ataaggcatt
     4201 tacgccgcat ccggcatgaa caaaactcac tttgtctgca atctgaatcc aggccaccat
     4261 gcccagttga ttctttattg ccagccgtaa cgacggctat aaaatccttt caccagttgg
     4321 gttaacgtca tataccctgc cagaatcgca accagccacg ggaaatagct taacggcagc
     4381 gcctgcagtt gcagataact ggccagcggt gaaaacggca atgcgatccc gacaatcatc
     4441 acgatcacgg tcatgatcat taacggccac gatgcacagc tctgaataaa cggcacacgg
     4501 cgggtgcgga tcatatgcac aatcagcgtt tgcgacagca agcccaccac aaaccatccc
     4561 gactggaaca gcgtttgcgt ttccggcgtg ttggcatgga atacccacca catcaggcaa
     4621 aacgtcagaa tatcgaagat cgagctgatc ggtccgaaga agatcataaa gcgccccaga
     4681 tccgccggat tccaacgctg cggcttttga atttgctcgt cgtcgacgtt atcaaacggg
     4741 atcgccacct gtgacacatc atacagcagg ttctgaataa gcaagtgtaa cggcaacatc
     4801 ggcaggaagg gcaagaaagc actcgccacc agcacgctga acacattacc gaagttggag
     4861 ctcgccgtca ttttgatgta tttcagcatg ttggcgaaag tgcgacgtcc ttcaataacc
     4921 ccctcttcca gcaccatcag gcttttttcc agcaggatga tatcagccgc ttcacgggca
     4981 atatctaccg cgccgtccac agaaatgccg atatccgcag cgcgtaaggc gggcgcatca
     5041 ttaataccat cgcccataaa gccaaccaca tgcccttcgc gcttcagtaa ggtcacaatg
     5101 cgttctttat gcatcggcgt cagacgagca aacagcgtgg tgcgctgtgc gagatttgcc
     5161 agttcgtcgt cagatagcgt ttcaatatca ctaccaatga ccacctctcc cgcgtccagc
     5221 cccacttcat ggcacacttt cgctgcaact aactcactgt cgccagtaag gatttttacg
     5281 gtaattccac tcgcttttaa tgccttcagt gccggagcag ttgtctcttt tggcggatca
     5341 aggaaagcaa tatatccttc gaggatcagg tcggattcat ccgcccgctg gtaatctcct
     5401 tcacgcgctg gcaggtattt cgtcgccacc gcaaccacgc gcagcccctg acgattcagc
     5461 gtatcagtaa cccgcttaat cttacgcagc atgatgtcat cgagcggcac aatctcgcca
     5521 ttgtgacgca cctgcgaaca cacattgagg atttcctgca atgcaccttt gcaaaccagc
     5581 tgatggtgct cggtattttc tgccactacc accgacatcc ggcgacgctc gaaatcaaac
     5641 ggaatctcat caattttttg ccaacggctg gccagagagc gcgctgactc ttcatccgta
     5701 ccttcgagca ccgctgtatc gagtaggttt ttaagtccgg tctgataatg actgttcaac
     5761 cacgcgctat gcagcacgcg ttcgctggtt ttaccggaga tatcggtatg attctccagc
     5821 acaattttgt cctgtgtcag ggtgccagtt ttatcagtgc acagaatatc cattgcgcca
     5881 aagttctgaa tagcatccag atgtttgacg atcactttct gtttcgacag ctttactgcg
     5941 ccgcgcgcca gcgtcgaggt aacaatcatc ggcaacattt ccggcgttaa gcctaccgcg
     6001 accgaaagcg caaacagtgc cgcttcccac cagtcgcctt tggtgtaacc gttgataagc
     6061 aacaccaccg gagccatcac cagcataaag cgaatcagca gcatactgac gcggctgatc
     6121 ccctgctgaa aggcattcgg ctcgctttcc tgctcactaa cacgccccgc cagttgacca
     6181 aaccaggtgt tggctcctgt agcaatcacc attgcttgtg ccgtgccgct caccacggtg
     6241 gtgcccataa aacacagggt gtcacactcc agcggattgc tgtgctccgg ctggcgagtg
     6301 gtagcggctt tttccacggg cagagactca ccggttaacg acgcctgagc gacgaacaga
     6361 tcccgcgcct gcaagatacg taaatctgcc gggatcatat cccccgccgc cagttttata
     6421 atatcgccgg gcaccagctg gtcgatcggg atctccagcc agccattttc gcctttgtcg
     6481 ttaattacgc gcagcaccgt cgcggtattg ctgaccatcg ctttcagggc atctgccgct
     6541 ttagtggaac gtgcttcctg aataaagttc agcaacgtag aaatagcgac cattagcgcg
     6601 ataacgcccg cggcaaataa atcttcagtg gcgtaagaaa tagcgccgag aatggtgagt
     6661 aaaatattaa atgggttgcg atagcagacc cataaatgta cccaccacgg cgacggttgt
     6721 tgtgcgggta atttattttc accatgttgt tcgcgggcag attccacttc cgcctgattt
     6781 aacccttccg gatgggtgtc gaacgttttc cacaattctt cttcgggcat caccgccatt
     6841 ttcaggcaat gcgcacttaa ggacggcggg accgccgtat tcactgtctg ctgcgcgcca
     6901 ggcaatggat cgcgatgaac cagacgggaa ggtaaatggc gaatgagccg ggtaaaaatt
     6961 tctttaaaca taaggagtcc ctccgcactg tctgaaacag tacagaaatg tcttcaggcg
     7021 tggcgaagcc ttacctgaag gtaataaagc tgagcaggga cgttaaaacg tcacggcctt
     7081 acgcatccgg tagcgccgtg aaaagacggg cttaccggaa aagttttaat ctccgtcgag
     7141 ggagaggcgt gggatcaggt tccatataac ctccggtaag tgaaaaaagc gccgcaaata
     7201 ataaggagaa tccaataata ttcgtagcat ttatggcgat ataatacttg ctggcaacta
     7261 aaccaacgat aaaccagact ttacccattg ctgaatgcac gggtaaagtt aggctcaaat
     7321 aattaaacaa cacgttacag gacaacagga tgcaaaatcg gctgactatc aaagacatcg
     7381 cacgcttaag cggcgtgggg aaatctacag tttcccgggt gctgaataac gaaagcggcg
     7441 tgagccagcg cacccgcgag cgtgttgaag cagtgatgaa tcagcatgga ttttcccctt
     7501 cccgctctgc gcgcgctatg cgtgggcaaa gcgataaagt ggtcgccatc attgttaccc
     7561 gtctggattc gttgtcagaa aatctcgccg ttcaaaccat gctgccagcg ttctatgaac
     7621 aaggttacga cccaatcatg atggaaagcc agttttcccc gcaattagtt gccgaacatt
     7681 tgggggtgct gaaacggcgt aatatcgacg gcgtagtgct gttcggtttt actggcataa
     7741 cagaagaaat gttagcccac tggcagtcat cgctggttct gctggcgcgt gacgcaaaag
     7801 gctttgcttc ggtctgttat gacgacgaag gggcaatcaa gatcctgatg caacggctgt
     7861 atgaccaggg gcatcgtaat atcagttatc tcggcgtgcc gcatagtgac gtgacaaccg
     7921 gtaagcgacg tcacgaagcc tacctggcgt tctgcaaagc gcataaactg catcccgttg
     7981 ccgctctgcc agggcttgct atgaagcaag gctatgagaa cgtggcaaaa gtgattacgc
     8041 ctgaaactac tgcgttactg tgcgcaaccg acacgctggc acttggcgca agtaaatacc
     8101 tgcaagagca acgcatcgac accttgcaac tggcgagcgt cggtaataca ccgttaatga
     8161 aattcctcca tccggagatc gtcaccgtag atcccggtta cgccgaagct ggacgccagg
     8221 cggcctgcca attgatcgca caggtaaccg ggcgcagcga accgcaacaa atcatcatcc
     8281 ccgccaccct gtcctgatcg tttcctgaac gataaattgt gatcttcgct gcatttcggg
     8341 aacgttcccg tttttaaatt tttccgcgca atatattctg cagccaacca aaaatgtcat
     8401 ctgccatggg gctttatgat gagcaaaata aaccaaacgg atatcgatcg gttgattgaa
     8461 ctggtcggcg ggcgcggcaa tattgcgacg gtgagccact gtattactcg cctgcgcttt
     8521 gtcctcaacc aaccggccaa tgccagaccg aaagaaattg agcaactccc catggtgaaa
     8581 ggctgtttca ccaatgccgg gcaatttcag gtggtgattg gcaccaacgt gggtgattac
     8641 tatcaagcac taatagcgtc aaccggacag gcgcaggttg ataaagagca ggtaaaaaaa
     8701 gccgcccggc agaatatgaa atggcatgag cagttgatct ctcatttcgc ggagatcttc
     8761 ttcccgttgc tgcccgcgtt gattagcggc ggtttgatcc tcggttttcg caatgtgatc
     8821 ggcgatttgc ccatgagcaa cggtcagacg ctggcgcaaa tgtacccttc cctgcaaacg
     8881 atctacgatt ttctgtggtt gatcggtgaa gcgattttct tctacctgcc ggtcgggatt
     8941 tgctggtcag cggtgaaaaa aatgggcggc acgccgatcc ttggtatcgt gcttggcgtg
     9001 acactggttt ccccccagct gatgaacgct tatctgctcg ggcagcagct gccggaagtg
     9061 tgggactttg gcatgttcag catcgccaaa gtgggctatc aggcgcaggt gatcccggca
     9121 ctgttagccg ggctggcgct gggcgttatt gaaactcgcc ttaaacgcat cgtaccggat
     9181 tacctctatc tggtggtggt gcccgtctgt tcgctgatcc tcgcggtgtt cctcgcccat
     9241 gcgctgattg gtccgtttgg tcgcatgatt ggcgatggcg ttgcctttgc ggtacgtcac
     9301 ctgatgaccg gcagctttgc tccgattggt gcggcattgt ttggcttcct gtacgcgccg
     9361 ctggtgatca ccggcgtaca ccagaccacc cttgctattg atttgcagat gattcaaagc
     9421 atgggtggca cgccagtgtg gccgctgatt gcgctgtcga atatcgctca gggctccgcc
     9481 gtgataggca ttatcatttc cagccgcaag cacaatgaac gcgagatctc cgtgcctgcc
     9541 gctatctccg cctggcttgg ggtcactgag cctgcaatgt acggcatcaa cctgaaatat
     9601 cgcttcccga tgctgtgcgc gatgattggt tctggtctgg caggattgct atgcggcctg
     9661 aacggcgtta tggcgaatgg tatcggcgta gggggcctgc cgggaattct ctcgattcaa
     9721 ccgagctact ggcaggtatt tgcgctggca atggctatcg ccatcatcat cccgattgta
     9781 ctcacctcgt ttatctatca gcggaaatac cgcttgggca cgctggacat tgtttaattt
     9841 tcttcggggc gcaattgcgc cccctcgcat ttgcaggaat aacgtaatga ctaatcttcc
     9901 ccactggtgg caaaacggcg ttatctacca gatttatcca aagagttttc aggacaccac
     9961 gggtagcggc accggcgatt tacgtggcgt tatccaacgc ctggactatc tgcataaact
    10021 gggcgttgat gccatctggc taaccccctt ttatgtctct ccccaggtcg ataacggtta
    10081 cgatgtagcg aactatacgg cgattgatcc cacctacggc acgctggacg attttgacga
    10141 actggtgacg caggcaaaat cgcgcgggat tcgtatcatt ctcgatatgg tgtttaacca
    10201 tacctctacc caacatgcct ggtttcgcga ggcgctgaac aaagaaagcc cttaccgcca
    10261 gttttatatc tggcgcgatg gcgaaccaga aacgccaccg aacaactggc gttcaaaatt
    10321 tggcggtagt gcgtggcgct ggcatgcgga aagcgaacag tactatttgc atctctttgc
    10381 accagaacag gcggatctca actgggagaa tccagcggta cgcgcagagc tgaaaaaagt
    10441 ctgtgaattc tgggccgatc gtggggtcga cgggttgcgc ctggatgtgg tgaatctgat
    10501 ctccaaagac ccgcgtttcc ctgaagacct ggatggcgac gggcgtcgct tctacaccga
    10561 cgggccacga gcacacgagt ttttgcacga gatgaaccgc gatgtgttta cgccacgcgg
    10621 gttaatgacc gtaggtgaaa tgtcctccac cagccttgag cattgccagc gatacgcggc
    10681 actgacaggc agtgaattgt cgatgacctt taattttcat cacctgaagg tcgattatcc
    10741 cggtggtgaa aaatggacgc tggctaaacc tgactttgtg gcgttgaaaa cattgttccg
    10801 ccactggcaa caaggaatgc acaacgtggc atggaatgcc ttgttctggt gtaaccacga
    10861 tcagccgcgc attgtttctc gctttggtga tgaaggtgaa taccgcgtgc ctgcggcaaa
    10921 aatgctggcg atggtgcagc atggcatgca gggaacgccg tatatctacc agggcgaaga
    10981 gattggcatg accaacccgc atttcacgcg cattactgac tatcgcgacg tggagagcct
    11041 caatatgttt gccgagctgc gcaacgatgg tcgtgatgcc gacgagttat tggcaatcct
    11101 cgccagtaaa tcccgtgaca acagccgcac gcccatgcaa tggagcaacg gcgataatgc
    11161 cgggtttacg gctggcgaac cgtggattgg cctgggcgat aactatcaac aaatcaacgt
    11221 agaagccgcg ctggccgatg actcctcggt gttttacacc taccaaaagt taatcgcact
    11281 gcgtaagcaa gaagccatcc tgacatgggg caattaccag gatctgctgc caaacagccc
    11341 tgtattgtgg tgctatcgcc gtgaatggaa ggggcaaacc ttgctggtca ttgccaacct
    11401 tagccgtgag atccaaccct ggcagccagg gcaaatgcgc ggcaactggc agcttgtgat
    11461 gcataactac gaagaagcct caccacaacc ctgtgccatg aatttacggc cttttgaggc
    11521 tgtctggtgg ttacagaagt aaatcttccc taagccccgg taatgccggt cattccgggg
    11581 ttttgtctgt cagcatgttg tttttgttga tttttcaacc agcaaattca ttaaaaaatt
    11641 tacataacgc tgtagcgccc gtcatccgta cgctctgctt tttactttga gctacatcaa
    11701 aaaaagttaa aacatccttg atgcaaagca ctatatatag actttaaaat gcgtcccaac
    11761 ccaatatgtt gtattaatcg actataattg ctactacagc tccccacgaa aaaggtgcgg
    11821 cgttgtggat aagcggatgg cgattgcgga aagcaccgga aaacgaaacg aaaaaaccgg
    11881 aaaacgcctt tcccaatttc tgtggataac ctgttcttaa aaatatggag cgatcatgac
    11941 accgcatgtg atgaaacgag acggctgcaa agtgccgttt aaatcagagc gcatcaaaga
    12001 agcgattctg cgtgcagcta aagcagcgga agtcgatgat gccgattatt gcgccactgt
    12061 tgccgcggtt gtcagcgagc agatgcaggg ccgcaaccag gtggatatca acgagatcca
    12121 gaccgcagtt gaaaaccagc tgatgtcggg tccatacaaa caactggctc gtgcttacat
    12181 cgagtaccgt cacgatcgcg acattgaacg tgaaaaacgc ggtcgcctga accaggagat
    12241 ccgtggtctg gtcgagcaga ccaacgcctc gttactcaac gaaaacgcca acaaagacag
    12301 caaggtgatt ccaacccagc gcgacctgct ggccgggatc gtggctaaac actatgcacg
    12361 ccagcacctg ctgccgcgtg acgtggtgca ggcacatgag cgtggcgata ttcactatca
    12421 cgatctcgat tactcgccgt tcttcccgat gttcaactgc atgttgatcg acctgaaagg
    12481 catgctgacc cagggcttta aaatggggaa cgccgagatt gaaccgccga agtcgatctc
    12541 tacggcaacc gcggtaactg cgcagattat tgctcaggtc gccagccata tttatggcgg
    12601 caccaccatt aaccgtatcg atgaagtgct ggcgccgttt gttaccgcca gctacaacaa
    12661 acatcgcaag accgcagaag agtggaacat cccggacgcc gaaggctacg ctaactctcg
    12721 taccatcaaa gagtgctacg atgccttcca gtcgctggag tacgaagtaa acaccctgca
    12781 caccgccaac ggtcagacgc cgtttgtaac ctttggtttt ggtctgggca ccagcaggga
    12841 atcgcgcctg attcaggaat cgatcctgcg taaccgtatc gcaggcctcg gtaaaaaccg
    12901 taaaactgcg gtgttcccga aactggtgtt tgcgattcgc gatggcctga accataaaaa
    12961 aggcgatccg aactacgaca tcaaacagct ggcgctggag tgcgcaagca agcgcatgta
    13021 tccggatatc ctgaactacg atcaggtagt gaaagtcacc ggttcgttta aaaccccgat
    13081 gggctgccgc agcttcctcg gcgtgtggga aaatgaaaac ggcgagcaga tccacgatgg
    13141 tcgtaacaac ctcggcgtga tcagcctgaa cctgccgcgt attgctctgg aagcaaaagg
    13201 cgatgaagcc accttctgga agctgctgga tgaacgtctg gtgctggcac gtaaggcact
    13261 gatgacccgt atcgctcgtc tcgaaggcgt gaaagcgcgc gtcgccccga tcctctatat
    13321 ggaaggtgct tgtggcgtac gtctgaatgc tgacgatgat gtttctgaaa tcttcaaaaa
    13381 cggtcgtgcg tctatttcgc tgggttacat cggcatccac gaaaccatta acgcgctgtt
    13441 cggcggcgag catgtttacg acaacgagca gcttcgcgcg aaaggtatcg ccattgttga
    13501 acgtctgcgt caggcagtgg atcagtggaa agaagaaacg ggttatggtt tcagtctcta
    13561 cagcacgccg agtgaaaacc tgtgcgaccg cttctgccgt ctcgatactg ctgagtttgg
    13621 cgtggtgccg ggcgtgaccg ataaaggtta ctacaccaac agtttccacc ttgatgtgga
    13681 gaagaaggtg aacccgtacg acaagatcga ctttgaagcg ccttacccgc cgctggcgaa
    13741 cggtggtttc atttgctacg gcgagtatcc aaacattcag cacaacctga aagcgctgga
    13801 agatgtctgg gattacagct atcagcatgt accgtattac ggcaccaata caccgattga
    13861 tgagtgctac gagtgtggct ttaccggtga gttcgagtgc accagcaaag gcttcacttg
    13921 cccgaaatgt ggtaaccatg acgcctcccg tgtgtcggta actcgccgcg tgtgcggata
    13981 tttaggtagc ccggatgcgc gtccgtttaa cgccggtaag caggaagaag ttaagcgccg
    14041 cgttaaacat ttggggaatg ggcagatagg ttaatcttca ctctgcgtta agcaaatgcc
    14101 ggatgcggcg taaacgcctt atccggccta catttggcaa cggctgtagg cctgataaga
    14161 cgcgacagcg tcgcatcagg cattgattac cggatgcagc ctacacgcgg actgataagc
    14221 caggcgaaat aatgaattat catcagtact atcctgtcga catcgtcaac ggccccggca
    14281 ctcgctgcac cctgtttgtc tccgggtgtg ttcatgaatg ccccggttgc tataacaaaa
    14341 gcacatggcg ggtaaattct ggtcagccat ttaccaaagc aatggaagac aagatcatta
    14401 aagatctgaa tgacactcgt atcaaacgcc aggggatttc cctctccggc ggcgatccgc
    14461 tgcatccgca aaacgtgccg gatattctga aactggtaaa acgcatccgc gccgagtgtc
    14521 cgggtaaaga catctgggtg tggacaggct ataaactcga cgaactcaac gctgcgcaaa
    14581 tgcaggttgt tgatttgatt aacgtgctgg tcgacggcaa atttgtgcag gatttaaaag
    14641 acccttccct gatctggcgc ggcagcagca atcaggtggt gcatcatttg cgatgataag
    14701 ttccctgtta cctgagtgca ggcaacaggg aaatgaggaa ttaacgatac ttctggtgat
    14761 aggcgttgcg ggtcgttttc agttgctctg cagcagcctg cgcttctttt actttacctt
    14821 catttgccag cttcagcgcg tcgtcaatct gaccgaccag aatgtcgaaa ccgtggcgga
    14881 aatctttcat ttccgggctg tccggtgatt tatcttcgag cttcggcggc gttgcttttt
    14941 gcgcatccag cgctgcggcg cgcatcttcg ttaacgcgtc tttgacttgc gtcgcgttat
    15001 cggctttttc gaccactttt aaattgtcgt tgagggtttc catattgtct tcaagatcag
    15061 cggcaaacga cgccgaactg aataccaacg aggagactgc aagaatagct aacaggcttt
    15121 tacgcattgc tcacttcctt ttttatttac acttcatcct tcacactgcc tcatcgacag
    15181 ctacgctcat tcgaccgggt cacgtactac gagtacgctc cccggctctc attcactttc
    15241 cgtctcgatg cagtgcgaat gattttgtgt aattttttag tttatagcgc ggcaggtcgc
    15301 gccagttttt ttactgtccg gcgattttca tctccggcaa cagcacagaa ccacactgta
    15361 tattactgcg tgtttcaata tcgttaccga cggtgacaat attgcgccac atatctttta
    15421 aattacctgc gatggtgatt tcgctcaccg gatactgaat ttcgccattc tctacccaga
    15481 agcccgcagc accacgggaa taatcaccgg taatggcact cacgccctgg cccatcagct
    15541 ccgtcaccac cagcccggtg cccatctctt tgagcatttg ttcgaagctt agcccttgtc
    15601 cggcaatccg ccagttgtga ataccgcccg catgtccggt gcttttcagc ccaagttttc
    15661 gcgccgaata gctggtcagc agccactgag tcaggatgcc atctttaata atatcgcgac
    15721 gctcggtgcg cacaccttcg ctgtcgaatg gcgtcgacgc cagccctttc agcagatgcg
    15781 gatgctcttc aatggtcagc cagtccggca gaatttgttt acccagcgaa tccagcagga
    15841 aggtagattt acgataaacc gatccacccg ctatcgcccc caccagatgc ccaaaaagcc
    15901 cagttgccac ttcattggca aaaatgactg gcgctttcat ggtggagagt ttacgcggta
    15961 acaggcgcga taaagtacgg cgagcacagt cggccccaac ccactctggc gtttgcagat
    16021 cgctcatcgc gcgcccaatg gtgtaggcgt aatcacgctc catatcgcca ttttcttcgg
    16081 caattacaca gctggagagc gaatgacgcg ttgagcagta accctgcaac atgccgtggc
    16141 tgttgccaaa aactttgaca ccgtagtggc tgttaaagct gccaccttcg gtattggtga
    16201 tacgtttatc cgcctgtaat gctgcctgtt ctgcgcgggc cgccagttca atggcttcat
    16261 ccggggaaac ttccgcaggg tggaacaagt cgagatctgg tgcgtcaaag gccagcagct
    16321 ctttgtctgc caggccggca cagggatctg gcgaggtata acgggcaata tccagcgccg
    16381 cctgtacagt acgggcaatg gcctgcgggc ttaaatcggt ggatgatgcg ctacctttgc
    16441 ggttctggtg ataaacagtg atccccagcg cgccatcgct attgaattca acattctcca
    16501 cttcaccata acgcgtgctt acgctaatgc cggtggtctt gctgacggca acttccgcac
    16561 cgtccgattt gcctgaggcc aactccagcg cagtggaaac tgcttcttcc agaatcttgc
    16621 gctgcgcttc aacttgagag attactttca ttgcaagtgc cataatgtag agagtttctc
    16681 tgaagtctaa cagagaaccg tttttcagtg cgcatcttaa ctggtaacat tagcctcttt
    16741 ttttaaggag cctgagatga ctaagcagcc cgaagactgg ctcgacgacg ttcccggtga
    16801 tgacatcgaa gacgaagacg atgaaattat ctgggtcagt aaaagtgaaa ttaaacgtga
    16861 tgccgaggag ctaaaacgcc ttggcgcgga aattgttgat ctggggaaaa acgcgctgga
    16921 taagatcccg ttagatgcgg atctgcgcgc ggctattgag ctcgcccagc gtattaagat
    16981 ggaaggtcgc cgccgccagt tgcagctcat cggtaagatg ctgcgccagc gcgacgtaga
    17041 gcctattcgt caggcgctgg ataagctgaa aaaccgtcac aaccagcagg tggtgctgtt
    17101 ccataaactg gaaaacctgc gcgatcgtct gatcgatcaa ggtgatgacg ccatcgctga
    17161 agtattgaat ctgtggcccg atgccgatcg tcagcaactg cgtacactga tccgcaacgc
    17221 gaagaaagag aaagaaggga ataagccgcc gaaatccgca cgccagattt tccagtatct
    17281 gcgcgaactg gcggagaatg aagggtaatt ttcgttgttg atgtgagtat ggtttgccgg
    17341 atgcggcgta aacgccttat ccggcaatgc tatatcaggc tgaagccgaa ttactgcgca
    17401 gcttccgcct tcttcgccag accatccagc agtttctgat ggatcccacc aaaaccgccg
    17461 ttgctcatca ccagaatatg gtcgccaggc tgagcggttt tcaccaccat atctgccagc
    17521 gtatccacat cgccactcca gtgtgcaggc tgaacgcagg cttctgccac ttctgccacc
    17581 tgccacggaa tatgcgccgg ttgcagcagg aagacttcat cggcacgacc taatgaaggt
    17641 gccagatcgt ctttgcagat ccccattttc atggtattcg agcgcggttc cagcacagca
    17701 ataatgcgcg ccgtaccacc aactttgcca cgcagcgccg ccagcgttgc cagaatcgcc
    17761 gtcgggtgat gggcaaaatc gtcatatacc gtgacgccat tcgcttcacc acgcaactcc
    17821 agacggcgac gagcattaat aaacgaaccc agcgcgttag cggcatctgc cgacgctaca
    17881 ccaacatggc gagccgctgc aatcgccatc aggccattgt gcatattatg ttcgcctacc
    17941 agcgaccatt tcacttcgcc cactttttcg ccatccagca aaacttccca ttcggaagca
    18001 tcggtggtca gctttttcgc ctgccagtga ccctgctcac ccaccagctc ctgctcgctc
    18061 cagcagccca tcgccatggt ctgtttcagg ttgatgtcat tttccggcca gataatacgg
    18121 ccctgccccg gaacgatacg caccagatgg tggaactgtt tctggatcgc tttcaggtcg
    18181 tcaaagatat cggcgtgatc gaactcaagg ttgttgagga tcagcgtacg cgggcagtaa
    18241 tggacaaatt tagagcgttt gtcgaagaag gcgcagtcgt actcatccgc ttcgataaca
    18301 aagaagtcgc tttcgcccag acgcgccgaa acctcaaagt tccccggcac accgccgatc
    18361 acaaatcccg gtttgtaacc acactgttcc agaatccagg tcgccattcc cgcggtggtg
    18421 gttttgccgt gtgtaccggc aacggccagc acccagcggt cgcgcagcac aaaatcgtgc
    18481 agccactgtg gacctgacat ataagggatg tttttttcca gtaccgcttc cacacacgga
    18541 tttccacggg tcatggcgtt gccaataatc accagatccg gctgcggctc gagctggctg
    18601 gcatcgtaac cctgaatcag ctcaatgcct tgcttctcaa gtaaggtgct catcggcgga
    18661 tacacattgg cgtccgaacc cgttacttca tggcctaact ggcgcgccag catcgccaga
    18721 ccgcccataa atgtgccaca aattcctaaa atatgaatgc gcatacgtca ctatcctttt
    18781 ttaatctgcc gctcatttta cgcatatgtc gggcaagtga gaaacgcatt tcaggataat
    18841 ccgcaatttg ctggcgcgat tcacctgagc gcaacattga gattatttgt taagattgtt
    18901 gcggtcgctt tactccataa acattgcagg gaaagtttta tgaaaacgtt aggtgaattt
    18961 attgtcgaaa agcagcacga gttttctcat gctaccggtg agctcactgc tttgctgtcg
    19021 gcaataaaac tgggcgccaa gattatccat cgcgatatca acaaagcagg actggttgat
    19081 atcctgggtg ccagcggtgc tgagaacgcg cagggcgagg ttcagcaaaa actcgacttg
    19141 ttcgctaatg aaaaactgaa agccgcactg aaagcacgcg atatcgttgc ggggattgcc
    19201 tctgaagaag aagatgagat tgtcgtcttt gaaggctgtg aatacgcaaa atacgtggtg
    19261 ctgatggacc ccctggatgg ctcgtccaac atcgatgtta acgtctctgt cggtaccatt
    19321 ttctccatct accgccgcgt tacgcctgtt ggcacgccgg taacggaaga agatttcctc
    19381 cagcctggta acaaacaggt tgcggcaggt tacgtggtat acggctcctc taccatgctg
    19441 gtttacacca ccggatgcgg tgttcacgcc tttacttacg atccttcgct gggcgttttc
    19501 tgcctgtgcc aggaacggat gcgcttcccg gagaaaggca aaacctactc catcaacgaa
    19561 ggaaactaca ttaagtttcc gaacggggtg aagaagtaca ttaaattctg ccaggaagaa
    19621 gataaatcca ccaatcgccc gtatacctcg cgttatatcg gttcactggt tgcggatttc
    19681 caccgtaacc tgctgaaagg cggtatttat ctctacccaa gcaccgccag ccacccggac
    19741 ggcaaactgc gtttgcttta tgagtgcaac ccgatggcat tcctggcgga acaagcgggc
    19801 ggtaaagcga gcgatggcaa agagcgtatt ctggatatca tcccggaaac cctgcaccag
    19861 cgccgttcgt tctttgtcgg caacgaccat atggttgaag atgtcgaacg ctttatccgt
    19921 gagttcccgg acgcgtaatc tcctgcgcct ctccacacgg agaggcgttt ttattgctgc
    19981 gcaatgttga ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaatcca
    20041 cgctgtaatg ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccaccaggaa
    20101 ctcagcgtgc catcaaagtt tatgtaagtc tgaatcagtc cctgaatcgc cacgccaaaa
    20161 agcgtcccta ataccgttcc aacgccaccg ctcaaaagcg taccgccaat taccactgag
    20221 gcgatagcat ccagctccac acctacgccc gccagcgcat atccggcctg ggtataaatc
    20281 gagaagacaa tccccgccag cgttgccagt ccggtggaga gcatataaat gcgaatagtg
    20341 gtgctgcgag tggaaatccc catcagattc gccgacgttg cgttgccgcc aatggcgtat
    20401 acctgattac caaaacgggt acgatgcgcg aggaatatgc cgataaccac caccgccaac
    20461 atcagcagtc ccatcgcact taagcgaccg ccgccaggga ttttccacgc aaggcttgag
    20521 agcgtgtcat aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg
    20581 ccgcgcaaaa agaacatccc cgcaagcgta atgatgaacg ccgggatctt cagggcgtcg
    20641 atcagaagcc ccataaatgc gccgaaggca cagcccatca ccagcaccag cggaaacgcc
    20701 agcagcggcg agaggccgaa atcgccaatc acttttgcca gaaacacgcc ggtaaaggcg
    20761 atcaccgaac cgacggagag atcgatccca ccggagagga tcacaaaggt catgccaacg
    20821 gcaatgatcc caagaaaggc gttatcggtc aggatattgc agatcactct tgtggaagca
    20881 aaaccaggaa actgcgtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc
    20941 ataagcggca agttacgttt tatcacggct acgcactcct ttaatcagac tgataaagcg
    21001 ttgcgactga acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc
    21061 tggcggaaag cccgaaagca aaattccggt attcattccc tgaataatca gcgccccaac
    21121 tcccgaaagc agcaggttaa agcgaccgcc catcagcgat ccaccgccaa tcaccaccgc
    21181 gagaatggca tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc
    21241 cgccgccacg ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat
    21301 gacgatgatt cgtgtgttga ctccggcatt ttttgccgcc cgaatgttga taccaacggc
    21361 ttcgataaac atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag
    21421 caccgcgata atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag
    21481 atccggcgag ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc
    21541 ggcgaccatc aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat
    21601 gccgttccac aatcccgcta ggatgccggt gcccagggcg cttaacaaaa caatcggcag
    21661 gctgaatccc gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc
    21721 cacagagaga tcgataccac cggtggcgat caccagcgtc ataccaatcg ccagtaacgc
    21781 aacgggagct gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag
    21841 caccacctgc cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc
    21901 cgccagctgc ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg
    21961 gagagattga ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa
    22021 cggaaagctg tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc
    22081 ggtcggcata gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt
    22141 cagcacacag cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac
    22201 gggttggctc atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca
    22261 ctttttgctg attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa
    22321 tgccaagctg gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc
    22381 gtagccagcc gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg
    22441 cgatgatgcc atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct
    22501 gatgtggcga tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag
    22561 gtttgatacc gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca
    22621 gaccgacgat ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc
    22681 cgtaattttt gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta
    22741 gcacgtgggt atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct
    22801 cgcgcgtttc ccgacagcct acgaaactgc cgttgcgtaa gacagtgatc cgatcgctga
    22861 cctgatagac ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca
    22921 actgacgcat caggccaaat aatagctcca cttcctgggt gtcgaggctg gcggtgggtt
    22981 catcgaggat cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct
    23041 gcatcgcgac tgaaaagcgg ttaagcggtt cgcgcacgtc gaggaagaaa ccgtaagatg
    23101 ccatcagttc ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg
    23161 gttcgcgacc tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct
    23221 gatagacggt gccaataccg agttgttgtg catgggcggt atttttcggt gagatagcct
    23281 ggccttccag ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta
    23341 gcgttgattt tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc
    23401 tgaaatcaac gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc
    23461 ggaggatctc ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca
    23521 gtaggcatga taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc
    23581 ggcgcgagcg ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc
    23641 atatttttct tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg
    23701 gttaacgtca gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa
    23761 gcgggtcccg ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc
    23821 gctttgtaga tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc
    23881 aggcccgctt ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa
    23941 accatgcaga tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg
    24001 cctttactgc gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc
    24061 ttaatggctt cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc
    24121 tgcagctcca ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc
    24181 aacttacctt cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc
    24241 acatcgatgg aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat
    24301 accggttccc aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttgtgca
    24361 acgaaggaac gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc
    24421 aacgtaattc cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag
    24481 ccggattccg atccgacctg cgaaaatcct ccggttaatg gagctgccaa cgccatagac
    24541 gacatggctg ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg
    24601 tcggggtaat tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg
    24661 taacggattg cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt
    24721 aatcgctttc atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat
    24781 accgtcttaa aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg
    24841 cgagttggct ataatactcg gcacttgttt gccacatatt tttaaaggaa acagacatga
    24901 gcttactcaa cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga
    24961 tcccggctaa cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg
    25021 ttgaccgctt catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca
    25081 ccctgtctct ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc
    25141 ctggttctgt gatccgttgc cgtccggttg gcgttctgaa aatgaccgac gaagccggtg
    25201 aagatgcgaa actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta
    25261 aagacgtaaa cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact
    25321 acaaagacct cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg
    25381 ctaaagctga aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc
    25441 aataaccctg aacgccgggc ttcggttagt aagggttttt tatgcccgcg ataaataaac
    25501 tctctattcc accatcatta ttctcagcgg ttgcaaggct tgaacggtaa gaacaagcaa
    25561 acccgaccac cattttgctg ttcatagcca cttgctggaa gttagccgac ctcactcata
    25621 ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat ttgtgaacgc cagcagagtt
    25681 ttttatgcga ttgcagatat ggaaagcggc tcggagagaa aagcaaaaga tggaaaagtt
    25741 acaaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaaggt ggcgtgcttt
    25801 tattattact tatccctgtc taaccagtcg ccgctttcaa ttagctttaa tccatcgacg
    25861 ggtcgttgat aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg
    25921 tattcaccgc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt gtcaatacga
    25981 taaacttcac cgtgtaccgt tccgttcccc ggaactgcgc ctggatagtg gcccaggcta
    26041 tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt catccagtga
    26101 ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc taaaactcga
    26161 actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga tatagcttag
    26221 gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc acgccgtatt
    26281 tcacttgcag acctggcaat acatagccgc tgaccactac ctgggaggag tcgcctactc
    26341 cctgggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta cccacaatct
    26401 ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca ctgtcgctct
    26461 gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt tgcgacatcg
    26521 ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg cgaactccgg
    26581 caattacgtc gtcttctgta gcatccgggt tacgaatagc ttcaatatta agatacggtt
    26641 gatccggcgg accagagaac agtaactcac ccttacgcac aatcagatcc tgaccatagg
    26701 catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct tgtttgtcct
    26761 gtacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat
    26821 tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt ttcggctctt
    26881 ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact gcgctttccg
    26941 gcagatcgtg caccacgatg cgcgcccacg ggacatccac gcgaccatcg agggtaaaca
    27001 ggtttggtgt agcctcgaat acaacatctg gcgatacatc cattcgtacc atcggcggca
    27061 cggtgatccg cactttactg cctttcgccg ttactcgtgc ccgccagttt tcaatttggc
    27121 tccagtcggc atcaccgttc aggtagattt caccctgctg ggtccgtact gtaccggcaa
    27181 gcgtcgagcg cataccgtta aagttgaccg caagctggct cggctgcata tcaaacggca
    27241 taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac tgcgggcttt
    27301 gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt tccccacggg
    27361 taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg ccaccaagat
    27421 tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg ttattggtca
    27481 ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc agattcagtg
    27541 tctgaaacgc caccggcagc gccgcatcgt tgacggtttg cgttacctgc acgttacgcc
    27601 ccgaaagggt gatactgccc tgcggcagcc cctctttcgt ggtgtcccag gcaacatccg
    27661 ctttacccgt gaagataccg ctggcctgag tggtttctgg cataaacggt ttcagcatgg
    27721 cgaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcccccgca tcgatagttt
    27781 gcggcacgca cagttccgca ttcgggttaa gccagcagtg tggcccgatg ctgatttttt
    27841 gctccttatt gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg ccgaccggcg
    27901 tctggaaacg ggtattgcta agagttccct tccagcgttc ttctttgcga tcaaaacttc
    27961 ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt agctcgtgct
    28021 gcttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc ggctgcgaaa
    28081 tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta atgtcgcctt
    28141 ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg gtgatatctg
    28201 ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc gctgtaccgc
    28261 caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca agattgagat
    28321 cttttacccc cagctcgcct ttcacttcgg cactgtttgg cccgagttcc agatgaagcc
    28381 cagggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg ttcactttgt
    28441 tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag gtgccgccgt
    28501 acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactcc gggaactctt
    28561 tggcggtgtt aatgccatta agcgttagct caccgcgcca actaatggcc tgctgccagt
    28621 cgagcaacgc cttgagttca gttttccctt ccagcgccgc gacggtgagt ttgtcgagat
    28681 tgacctgctg ttcattacct ttggcatcaa gggtaatggt ggctggcggg atctcctgtc
    28741 ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta agtttcagtt
    28801 tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaagc tgtttgctgt
    28861 tcacttccac gttgagtggc aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca
    28921 tatccaccgg accggaaagg ttaacgccta tctccagctg ttcgcgcagc gcgccgccca
    28981 ctttcagctt caccttttca cctttcaacg gctccacgtt cagtgtgctg ttgaggatga
    29041 tatccaccgg ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg atcccctggt
    29101 tggaatcgat atccagggcg tccagtttag tattgccgtc aatgctgctc actttcagca
    29161 gcatggtgcg cacggtgatg tccgtgtcgc ccgtcacgcg cagctgctcg cccttaaact
    29221 cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat accgggcgag
    29281 aaaagagatc tttcagcgtt tcgccgagcg gcttttcatc cggctgcgga ttttcaatct
    29341 tcggttcgac gacctcttcc tgcgccactt ccgccacttt cggcagagca atcagcaggc
    29401 ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcagg ccggaggtga
    29461 agtccatcac cgacaccgtg gtgtcatcaa tcttgatgtt gacgttatcc agcgccaccc
    29521 gtgtcagggt gatgggatac ggcgtggaga gatccagcgg accgctatct tcctcttctt
    29581 caacctgttc agaaggaggc atttttttac tgtcgatgtt gacctgaatg tctttcagcg
    29641 ccaggtcatt aatacaaacg ctactgttcc acaggcactc aagaccgacc gccagatgca
    29701 gattacccgc ttttaccgcc acgcctggct gctcataacg aacgtcagac aaggtgagat
    29761 cgcgccagcc gccggtgacg ttgccaatat ccagtcctgg cacccagcga tccgccgctt
    29821 taaatacaag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca
    29881 gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata attctggccc
    29941 cagaccgatg taaaactgta acccgtgttc gtctttatcc gcgaccggta cggcaaaatc
    30001 gagtttgatt ggcccgaccg gcgattccca gcgtacgccg cccccggtac cggttttaaa
    30061 gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg cgccccacca
    30121 ttttccggtc acgttgtact ggtattccag cgatccggtt atcagcttcg aggccccttt
    30181 caggtcaccg ttggcgtatt tcggggcgat agatttatat ttgtagccgc gaatactacg
    30241 atcgcctccg gcgaagaaac gcagatccgg cggtactttg tcgaaatcac cggtttcaat
    30301 ccagcccagc gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc ggatccagac
    30361 gttctgcgcc tggaaaacgg agaaatcaac atctgaaccc caggccgtgt tggagtagtc
    30421 gatagagtag cgttgcgagt cgccccaggt tggcatcagg ccaccacgag aacgcgtgcg
    30481 gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac cctgggtaaa
    30541 gtggtcgagg ctccagcgca ggttaatggc acgctgccag tcgctggaga gatcccagta
    30601 gcgagaagcc accagcgtag tggagtcaga ttcggtatcg ttcaggtcag tgcgcttaaa
    30661 accgccctgc accaaataat attgttccag tggattcttc agcagcggca ttttatagct
    30721 gaagtcgagg atctgttccg gcgcggaaat actggtactg gtggtcagac tgtgaccata
    30781 agagttcatc cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta
    30841 accgaccccg gtttcgatgg tgttttctgt tcgcggcgaa accacgcccg tcaatggtaa
    30901 tactttcgtt tcgcgcgctt tatcaaattg cggtgccact accaccgaat taaaccagcc
    30961 ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcatact catcgccctc
    31021 tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt caaaggtcac
    31081 atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg ctttatgcag
    31141 gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac ctttacgcaa
    31201 cgcaatactg gttaaggact ttttgaaatt ttcataatcg ccctggttca gtaccgtacc
    31261 aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg cgccgccgcg
    31321 caataccaca tcggtgccgc caattaacac cggtacacct ggcgtgactt tggcgatcaa
    31381 tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg gctggtaata
    31441 acccagcgct ttcagacctt cacggatggc atcatcgacg cgtgcgcgaa agcgacggtc
    31501 tggcgtcact tcatcacttt caatcgtaga aagctgcgca cgaacgttct tttccagctg
    31561 tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc cgcttaagca
    31621 gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg aatatccttt
    31681 tttcctaccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata tggattaaaa
    31741 agcagactaa accccaaata tttcttatgt tttactttag acctattcac ggtggttatt
    31801 gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg accgattttc gggagagcga
    31861 caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc tgcctggacg
    31921 taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa tgaccaatgt
    31981 acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg tggagcgtct
    32041 gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggcg gctatacgcc
    32101 aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag cggtacgcat
    32161 tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt gggagaatca
    32221 cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc gttcagcgat
    32281 ttatccgctg acccctgaac aggatgccgc agctcgcgcc agtctggaac gttttcaggc
    32341 ggcgatgctt gccgccgatg acgatcgtca catcaccacg gaaatcgcta acgccacacc
    32401 gttttattat gccgaagatg accaccagca atatctgcat aaaaacccat atggttactg
    32461 tggaattggc ggaattggcg tctgtctgcc gccggaagca tagcgttacg ggtacaaatg
    32521 tagtttgttg ataaagtgct ctttgtctat gcctgatgcg gcttaaacga cttaccaggc
    32581 ctacaaaatc gtccaaattc aatatattgc aaggactgcg taggcctgat cggcatagcg
    32641 catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg actttacagt
    32701 accttacgct atactagcca ctgaaaatgt cggttcactt tcttcgaatc ggctttcaat
    32761 gtgtatttca cacaaattaa tcaactttcc cttccgagga tctggcctga aaggtcggat
    32821 aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt gcgttcttct
    32881 cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg ctggctgatg
    32941 aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc atgttcttta
    33001 ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg
    33061 cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagag ctctctgagc
    33121 aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg tttgcggatt
    33181 taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt atcatcaacc
    33241 cgatgcgctt ctgcctgtac gtttgcaccc cactggtgtg gttcttcaac ggcctggcga
    33301 acatgatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc acttctgatg
    33361 acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg
    33421 agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca atgacaccgc
    33481 gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag aataaggtgg
    33541 cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac atcatcggtt
    33601 atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca
    33661 gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt tcagaggcgt
    33721 tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag tacgcgctgg
    33781 tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat ctggtcggtc
    33841 aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt gacggcggca
    33901 ctccgattga cgacgtcatg cgcgtgctgg atattgacga attcccgcag tcgggcaact
    33961 acgaaaccat cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt
    34021 cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac cgcatcgacc
    34081 agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg
    34141 ctaaagataa agaagaaagc gtcgcgtaac cccccagaaa catcaacggc tcctgaatca
    34201 ggagccgtct tattactgca tagcactttt tactgcatag cactttggtt aagccatctc
    34261 tgtttgcaga cgcataacct gacggttaac ttcggacatc actgacaaat gcagcttatc
    34321 cttcactttt gggataagaa tcttaccctt atcaaattca aaagcgccaa cgtccttgat
    34381 gtataaccgt ccacggaaca ggatctttac gtacttcgcc acctgaagtg ggttgtaccg
    34441 ttggaaaatt ttcattgtta tctcctgctg agtattacgc ctgtgcggtg ccacaatcgg
    34501 cccaactatt atgaggcgca aattttaatg cttagtgact atagactatc cgggcaatgt
    34561 ttccaccgcg tataactttt tttacctaaa ggttacaatt attcagaatt atctttttac
    34621 cgaagcgcgg tcttcagtat aagcattcat ttttcatatg atttgtgcgc ttgaccgcaa
    34681 actggcatca cacttgcggg aaattcgata aatagcacat atgattaaaa ctcagaccca
    34741 agtggtcgga tcacctgcat atcataagaa ggaaacacca tgaccctacg caagattctg
    34801 gcactcacct gcctgctgtt gccgatgatg gcttccgcac atcagttcga aaccggtcag
    34861 cgagtgccgc cgattggcat taccgatcgg ggcgagttgg tgcttgataa agatcagttt
    34921 agctacaaaa cctggaacag cgcgcagtta gtgggaaaag tgcgagtact gcaacatatt
    34981 gctggtcgca cctctgcaaa agagaaaaac gcgacgctga ttgaagcgat taaatcagcg
    35041 aagttaccgc acgatcgtta ccagaccacc actattgtta acaccgacga cgcgattccg
    35101 ggttcaggga tgtttgtgcg cagcagtctg gagagtaata aaaagcttta tccctggtcg
    35161 cagtttattg tcgatagcaa tggcgtcgcg cgcggtgcct ggcagctgga tgaagaaagt
    35221 tccgctgtag tggtgctgga taaggatggt cgcgtgcaat gggccaaaga cggggcgctc
    35281 actcaggaag aggtgcagca agtgatggac ctgctgcaaa agttgctcaa ataatggcaa
    35341 caacatagat tctgcaaaaa taattttccc ctatcgttat ctatgggata ggggaattaa
    35401 gtctctgagt tcttacgccg aactcatact ggtaacgcta gttgctcttt tttaaacgcc
    35461 tcaatggtcg acttatattt cttgcaaatc ttttctttgg taatattact atctgcggcc
    35521 agattcgcac ttactatcgc atccacttgt tcggctaatt ccacttgttg gcaacctgca
    35581 ttaaccagaa aattataaat aagggagtaa tcttcatcct ccaaaacaac agaggaacca
    35641 ttacgaaaac gttcatcccc tccatagatt ttaagcgcaa agcccaaaga ttctatgact
    35701 atggcaaaat tatcatcaat tattttttcg tgaatatacc atccatcgca ttctttaatt
    35761 ccacctcctg gtatttggag ttcaaattta ccccgtttta atttgcttac tatatcctcc
    35821 aataaggcat aacctaaaga cacgtcacca atcgcctggt gaatataacc tttaatcctt
    35881 ttcggtatgt cagactgttt tttctgcaga atatcacagg catacaatct tctgttttgt
    35941 tctaacggaa actcgcggtt tttgaaatcc atgaatgagt tatactcatt tattgttatg
    36001 ggaatttctt cgtattcaaa tccactacct cgcaatgtca ttgtatattc ggcaccatta
    36061 tctttaaggc tgaaactgat aaggaactta tccatggcgt taacagttcc attttttgat
    36121 gaaagtacct gataactatt tttatcaatc tgagttgcgc aacgaaagaa ctccctcaaa
    36181 gttaacaggc tatcataatt cgaaatccaa tcttttttct taaatgcatg ctggatattg
    36241 ttaaaaaaat tgtttgaatg tgtttttgta tacgatgact ttacaaataa ctccaccgca
    36301 ccaataaaat tattgtgtct gatcctgggt aacatatgac aaccttattt tatttggtta
    36361 ttttatgtcg ccattaaata tctcattaga gaagtaaact atcaaaaaac gctcattatt
    36421 aaaatgtaac ttattagcaa ttgaattttt gcactttaaa tacaatctct ttttatttat
    36481 aactttttag gttgggagaa atcaggatgg cagaatcagg aaatacactg tttctgccat
    36541 ctgaatttag taaatagaca ctctgaaccc cggattcagg aacgactcgc gcggagtgta
    36601 atccagcggt ttaccctgcc agtcgtgaac gtgcgctccg gcagctgcag ccacagcatg
    36661 tccagcggcg gtgtcccaaa tattcgttgg tccgaagcgc gggtacagct gcgcctgtcc
    36721 ttccgccacc aggcagaatt tcagcgaaga gccgatggac gtggtctgat gttcgccaag
    36781 ctgttgcaga tactctttca gctccgcatc tgcatgggaa cggctgataa ccaccagcgg
    36841 cgggcgcgca tcgcggacct gaatctgctt gcgcacaccg cactcttctt tccaggcctt
    36901 gccttctgcc gcgctgtaca ttacgttcat taccggcgca tacaccacac ctaaaatcgg
    36961 tttgccatgg tcaatgagcg caatgttaac ggtgaattcg ccattacgtt taataaactc
    37021 tttagtacca tccagcgggt ctaccagcca gtaacgctgc cagtgctgac ggacttccca
    37081 accgggagga tcttcttcag aaaggaccgg aatatccggt gtcagcgtac gtaaaccgtc
    37141 catgataacg gtgtgtgcgg caatatccgc tgccgttacc ggagaattgt ccgctttgct
    37201 gacgacgtcc atcggtttcg tcccgtcgta gacctgcata atggcatcgc ctgcattccg
    37261 tgcaagctgg catacttgat ctaacatttc tccacctcgt ctctgtgagc ggtgttaact
    37321 tattgtttta cttataccct atcgttaatg aatgcgccaa ctgtgatagt gtcatcattt
    37381 tcaaagcgta aaattgtggc attcttcact gttctataag taagacgttt attctccctt
    37441 ttctttcgta ttcccgatga taaaaggatg tccctgatga ttaagtttag cgcaacgctc
    37501 ctggccacgc tgattgccgc cagtgtgaat gcagcgacgg tcgatctgcg tatcatggaa
    37561 accactgatc tgcatagcaa catgatggat ttcgattatt acaaagacac cgccacggaa
    37621 aaattcggac tggtacgtac ggcaagcctg attaacgatg cccgcaatga agtgaaaaac
    37681 agcgtactgg tcgataacgg cgatttgatt caggggagtc cgctggcaga ttacatgtcg
    37741 gcgaaaggat taaaaacagg tgatgttcat ccggtttata aggcgctgaa tacgctggat
    37801 tatacggtcg gtacactcgg caatcatgaa tttaactacg gtctggatta cctgaaaaat
    37861 gcgctggcgg gagcgaaatt cccttatgta aatgccaacg tcattgacgc cagaaccaaa
    37921 cagccaatgt ttacaccgta tttaattaaa gataccgaag tggtcgataa agacggaaaa
    37981 aaacagacgc tgaagattgg ctatattggc gtcgtgccgc cgcaaatcat gggctgggat
    38041 aaagctaatt tatccgggaa agtgacggtg aatgatatta ccgaaaccgt gcgcaaatac
    38101 gtgcctgaaa tgcgcgagaa aggtgccgat gtcgttgtcg ttctggcaca ttccgggctg
    38161 tctgccgatc cgtataaagt gatggcggaa aactcagttt attacctcag tgaaattccg
    38221 ggcgttaacg ccattatgtt tggtcatgct cacgccgttt tcccgggtaa agattttgct
    38281 gatatcgaag gggctgatat caccaaaggc acgctgaatg gtgttccggc ggtaatgccg
    38341 ggcatgtggg gcgatcatct tggggtggtc gacttacaac tcagtaatga cagcggtaaa
    38401 tggcaggtga cgcaggcgaa agcggaagct cggccgattt acgacatcgc caataaaaaa
    38461 tccctcgcgg cggaagacag caagctggta gaaacactca aagccgatca cgatgccaca
    38521 cgccagttcg tcagcaagcc aatcggtaaa tctgccgaca atatgtatag ctatctggcg
    38581 ctggtgcagg acgatccgac cgtgcaagtc gtgaacaacg cgcaaaaagc gtatgtcgaa
    38641 cattacattc agggcgatcc ggatctggca aaactgccgg tgctttcagc tgccgcaccg
    38701 tttaaagttg gtggtcgcaa aaatgacccg gcaagctatg tggaggtgga aaaaggccag
    38761 ttgaccttcc gtaatgccgc cgatctttat ctctacccca atacgctgat tgtagtgaaa
    38821 gccagcggta aagaggtgaa agagtggctg gagtgctccg ccggacagtt taaccagatt
    38881 gatcctaaca gcacgaaacc acagtcactc atcaactggg atggtttccg cacttataac
    38941 tttgatgtta ttgatggtgt gaattatcag attgatgtta cccagcccgc ccgttatgac
    39001 ggcgagtgcc agatgattaa taccaatgcg gaaaggatta agaacctgac ctttaatggc
    39061 aagccgattg atccgaacgc catgttcctc gttgccacca ataactatcg cgcttacggc
    39121 ggcaaatttg ccggtacggg cgacagccat atcgcttttg cttcaccgga tgagaaccgc
    39181 tcggtgctgg cagcgtggat tgctgatgag tcgaaacgtg cgggggaaat tcacccggcg
    39241 gcagataaca actggcgttt agcaccgata gctgccgata agaaactgga tatccgtttc
    39301 gaaacttccc cgtcagataa agccgcagcg tttattaaag agaaagggca gtatccgatg
    39361 aataaagtcg cgaccgatga tatcgggttt gcgatttatc aggtggattt gagtaagtaa
    39421 aacacttctt tttcggccta taaatcatca atcgcatccg gcatttattg gcggatgcga
    39481 tgctggcgca tcttatccgc cctacaagtc atacaccgta ggccgcgcat ccggcagtta
    39541 tgccgcacgt tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc
    39601 cagtgcagca accttttcgc tcacctgctc gcccagcggc gtgaggctgt attcaacatg
    39661 cggtggcacc accggatacg cgatacggtt aagaaaacca tcctgttcta acgcctgtaa
    39721 cgactgcgca agcatctttt cgctcacccc gccgattttg cgccgcaggt cgctaaagcg
    39781 atgagtacct tcgcgtagcg ccaccagaat caacaccccc caacggctgg tgacgtgttt
    39841 caacacctcg cgcgacgggc actgttccgc aaagagatta ccctctttca gttgttgcga
    39901 caggctaacc tgactcattt catacttacc tttttgtacg tacttactaa aagtaagttt
    39961 aggtgttagc gtatttaaac acaagacaaa acgatggaga cttcccatga tcgctattac
    40021 tggtgccact ggccaacttg gtcactatat tattgaatcc ttgatgaaaa cggttcctgc
    40081 cagccaaata gtggctatcg ttcgtaatcc ggcaaaagcc caggctctag cagcacaagg
    40141 cattaccgtg cgtcaggctg actacggcga tgaagccgca ctgacatctg cacttcaggg
    40201 agtggaaaaa ctactgctga tctcttccag cgaagtgggt caacgtgccc cgcagcatcg
    40261 taatgttatt aatgccgcaa aggcggctgg cgtgaaattt atcgcttata ccagcctgct
    40321 acatgcagat acctccccgc tcggcctcgc cgatgagcac attgagacgg agaaaatgct
    40381 ggctgattct ggcatcgttt acaccctgct gcgcaacggc tggtacagcg aaaactacct
    40441 cgccagcgcc ccggcagcac tggaacacgg cgtatttatc ggtgcggcgg gcgatggcaa
    40501 aatcgcctca gcaacgcggg cagattatgc ggcagctgcg gcacgcgtga ttagcgaagc
    40561 cggtcacgaa ggcaaggttt acgaactggc gggcgatagt gcctggacgt tgacacagtt
    40621 agcggcagag ctgaccaaac agagcggcaa acaggttacc tatcaaaatc tgagcgaagc
    40681 cgatttcgcc gcggcactga aaagcgtcgg actgcccgac ggactggcgg atatgctggc
    40741 ggattctgac gttggcgcat cgaaaggtgg tctgtttgat aacagcaaaa cgcttagcaa
    40801 attgattggc cgcccaacga caacgttagc cgaaagcgta agccatcttt ttaatgttaa
    40861 taactagtta attaaagtgg catcctcccg catcctctct gataatgacg ggatgccggg
    40921 agcaatcatg tctgcttcct gaactttctt ctgacagacc aatggatgcc agtaatgatt
    40981 agcggcgtgc tgtacgccct gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg
    41041 ttgatcgtac cggaataccc ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg
    41101 ttaattgcgc tgcccattgc ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgc
    41161 gactggctta ccgccttgat gctcaccatg atgggcaacc ttatctatta cttctgcctt
    41221 gccagtgcca ttcaacgtac tggcgcgcct gtttccacga tgattatcgg caccctgccg
    41281 gtggtgattc ccgtttttgc caatctgctt tatagccagc gcgacggcaa actcgcatgg
    41341 ggaaaactcg ccccggcact ggtttgtatt ggcatcggcc tggcgtgtgt gaatattgct
    41401 gagttaaacc acggactccc cgattttgac tgggcacgtt atacctctgg catcgtgctg
    41461 gcgttagttt ccgtggtctg ctgggcgtgg tatgccctgc gcaacgcccg ctggctgcgg
    41521 gaaaatcccg acaaacatcc gatgatgtgg gcaacggcgc aggcgctggt cacactgccg
    41581 gtttctctca tcggctatct cgtcgcctgt tactggctga atatacaaac gccggacttc
    41641 tccttacctt ttggcccccg tccgctggtg tttattagtc tgatggttgc gatagccgtg
    41701 ctttgctcat gggttggcgc actctgctgg aacgtcgcca gccagcgatt accgacagtg
    41761 attctcgggc cgctgattgt tttcgaaacg ctggcaggtt tgctgtacac ctttttactc
    41821 cgccagcaaa tgccgccgct aatgacgctg agcggtatcg cgctgttagt gattggcgtg
    41881 gtcattgcgg tcagagcaaa accagaaaaa cctttaactg aatctgtctc agaaagttga
    41941 cacgctggca gtgagttaaa taagcctctg ctacgtaagg gttatagctt ttgccttaaa
    42001 gatgcattta aaatacatct tatattatta agaatgaggt atcagctatg gcttatcgcg
    42061 accaaccttt aggtgaactg gcgctctcta ttcctcgcgc ttcagctctg tttcgtaaat
    42121 atgatatgga ttactgctgt ggcggtaagc aaacgctggc gcgcgcggcg gcacgtaaag
    42181 aactggatgt tgaggtcatt gaagctgaac tggcaaagct tgctgaacaa ccgattgaga
    42241 aagactggcg tagcgccccg ctggcagaaa tcattgacca tatcatcgtg cgctaccacg
    42301 atcgtcaccg cgagcaactg ccggagctga tcctgcaagc gactaaagtc gagcgcgttc
    42361 acgccgacaa accgagcgtg ccaaaagggc tgacaaaata cctgactatg ctgcatgaag
    42421 agctatccag ccacatgatg aaagaagagc agatcctctt cccgatgatc aaacaaggca
    42481 tgggcagcca ggcaatggga ccaatcagcg tgatggaaag cgagcacgat gaagcgggcg
    42541 aactgctgga agtgattaaa cacaccacca ataacgtcac accgccgccg gaagcctgca
    42601 ccacctggaa agcgatgtat aacggtatta atgaactgat tgatgacctg atggatcaca
    42661 tcagcctgga aaacaatgta ctgttcccac gcgcgctggc gggtgagtaa tctaaaagct
    42721 ggatggcatt gcgccatcca gcatgataat gcggttattt ccgcagttca gcagcccgct
    42781 tcttaccaat aaacaaccag cccagcccca gtgcgataaa ccacagcggg gtgaccaaca
    42841 gcgcctggcg agtgtcatct tccagtgtca gcaacacgac cacgaacaca aagaacgcca
    42901 tacataccca gcacatcagc ttgccgagcg gcatcttgta gatcgacttc tcatgcagat
    42961 gaggacgctg tttgcggtaa accaggtacg agcaaaggat aattgtccag acgaacataa
    43021 acagaatcgc ggaaacggtt gtaatcatcg tgaacgcgcc aatcacgcta ggattcacat
    43081 acaacatcac cacgccgccg agcagacaga tacacgagaa ggtcaaccct ttcgcgggta
    43141 ctgcgcgctt agaaagttta gcgaacgctt tcggtgccac accttcctgc gccagaccaa
    43201 acagcatacg gctggtagag aagacgccgc tgttagcgga agacgccgca gaggtcagca
    43261 ccacaaagtt gatcacgctg gcagccgcag gcaaacctac caacacaaac agttcaacaa
    43321 acgggctctt ctccgggact accgaactcc acggcgtcac ggacataatc acaatcagcg
    43381 cgaagacgta gaacataatg atacggatcg gaatggagtt aatcgcgcgt ggcagtgatt
    43441 tctccggatc tttggtttcc gcagcggttg tacctaccag ctcaatccct acgaaagcga
    43501 aaaccgctat ctggaatccg gcaaagaagc cacttaaacc tttcgggaac cagccgccat
    43561 cattccacaa atgcgcgaat gacgcttcca caccagtcgg tgactgaaag tgcatcgcca
    43621 ccataaccag gccgacgaca atcagcgaca caatggcgac gattttgatc atcgcaaacc
    43681 agaactccat ctcaccgaac attttcacgg tggcgagatt gagcgtcagc agcagcacta
    43741 tcaccgccag cgaggcgacc cagtcggaga gatcggggaa ccagaactga gcataagcag
    43801 tgatcgccac cacgtctgcc ataccggtta caacccagca gaaccagtaa gtccagccgg
    43861 tgaaatatcc tgcccacggc ccgagtaaat cggaagcgaa gtcactaaaa gatttgtatt
    43921 ccagattcga aagcagcaat tcccccattg cccgcatcac gaaaaagagc ataaaaccga
    43981 tgatcatata aacgaaaatg atcgacggcc cggcaaggct aatcgttttg ccggacccca
    44041 taaacaaccc cgtaccaatg gcaccgccaa tggcaataag ctgaatatgt cggtttgtga
    44101 gattgcgccg tagcgactgt tcagccggag cctgatcatc ggcaacgact tttacctgat
    44161 ctaccatgtt tttttcttcc tgtacctgtc tgtgttgttc aggctctacg gcctttagtc
    44221 agtctatgat acgacgaatc tgtatcggga ctcatcgata ttaggtaaca atacgcggga
    44281 tgaatactaa gatttagatc taatgttaat tttatgttta aagtgagtgt catatcacgg
    44341 tgataacgcg aaacagctca caaaaataca cgcttacggg gtctttgcaa cacaaaatag
    44401 cgccgtttgg caaactgaag ggtttattgc tgaatgcctg ctcccctctg gttcgagggg
    44461 aacagaatgc gctgcttaga ggatttccag cagttcgact tcaaacacca gggtgctgaa
    44521 cggagggatg gatgcgcctg cgccgcgctc gccatatgcc agttcctgcg ggatagtcag
    44581 ttcccattta gaacctaccg gcatcagagt cagtgcttca atccagccag ggatcacgcc
    44641 attaaccggg aattcagcgg gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc
    44701 gatcagttta ccggtgtaat gaacacgaac gcggtcggta cgtgccggaa ttgcgccttc
    44761 accctggttg atcacgcgga attgcaggcc agattcggtg ctattcacac cttctttttt
    44821 ggcgttttct tccaggtatt tcacaccttc agcagccatc gcctggaaac gctgacgacg
    44881 aacggcatcg gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat caaccggcac
    44941 agccggatgt ttgccttcca gcgcatcggc aatacctgca accagtgctt ctggcagcag
    45001 cccttccagg ccagattcac tcagttgttg ccctacctgc aaaccaatgc cgtagcttgc
    45061 ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta tttcctttct tagggtataa
    45121 aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc aggaatgatg
    45181 acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat aaaggcgggt
    45241 ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa caagacgcgg
    45301 agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag aaagtctggc
    45361 acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt cgggggatca
    45421 ttattgccgc cattgtattg gtggtcggat ttctgcttcc atctgatgat acgcccaacg
    45481 cgccggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa ccgccgacag
    45541 aagaacagct gcgggcgcaa ttagtcacac cacaaaatga tcctgatcag gtggctccgg
    45601 tcgctccaga acctatacag gaaggccagc cagaagaaca gccgcaaact acccaaacgc
    45661 aaccattcca gccagatagc ggaattgata atcaatggcg ctcttaccgt gtcgaacccg
    45721 gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc cgctaccgat gtctatgcaa
    45781 tggcgcaggt agaaggcgcg ggtaagccgc tgagtaattt gcaaaatggg cagatggtga
    45841 aaatccgcca gaacgccagc ggcgtggtga cgggcttaac cattgatacg gggaataatc
    45901 aacaggtcct gtttacccgc cagccggatg gcagttttat tcgtgcgcgg tagatgaagg
    45961 gtcaaccaaa agccagctaa ggattgtctc tttgatgacc ttacgtctgg cttccagtgc
    46021 ctggggtgaa accatactga tgtgataaac caggccaagc gtatgctggt tgatcaaata
    46081 atatccccca agcgcagcaa tattaatatt cacctgcaat gggtcgatat ctggcttaaa
    46141 aatattgtgt ttcttccctt catccaataa ggattccatt aactgcaagt gcgcatggtt
    46201 aatttctaag agacgcagtg attttgcata atgcacacct ttactttgat tttcactatg
    46261 cacaattttt aaaaaccaag ggtt
//
LOCUS       DAJTXQ010000014        45922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000014 DAJTXQ010000000
VERSION     DAJTXQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.014"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..715)
                     /locus_tag="OO515_000766"
     CDS             complement(<1..715)
                     /locus_tag="OO515_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6574780.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="OO515_000767"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="OO515_000767"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6574781.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="OO515_000768"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="OO515_000768"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6574782.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="OO515_000769"
     CDS             complement(3649..4704)
                     /locus_tag="OO515_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6574783.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="OO515_000770"
     CDS             complement(4714..5502)
                     /locus_tag="OO515_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6574784.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="OO515_000771"
     CDS             complement(5520..6572)
                     /locus_tag="OO515_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6574785.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7206)
                     /locus_tag="OO515_000772"
                     /pseudo
     CDS             complement(6603..7206)
                     /locus_tag="OO515_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7193..8074)
                     /locus_tag="OO515_000773"
     CDS             complement(7193..8074)
                     /locus_tag="OO515_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6574786.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8095..9405)
                     /locus_tag="OO515_000774"
     CDS             complement(8095..9405)
                     /locus_tag="OO515_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6574787.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9440..10950)
                     /locus_tag="OO515_000775"
                     /pseudo
     CDS             complement(9440..10950)
                     /locus_tag="OO515_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="OO515_000776"
     CDS             complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="OO515_000776"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6574788.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="OO515_000777"
     CDS             complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="OO515_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6574789.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="OO515_000778"
     CDS             complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="OO515_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6574790.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="OO515_000779"
     CDS             complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="OO515_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6574791.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="OO515_000780"
     CDS             complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="OO515_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6574792.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13253..14230
                     /gene="pspF"
                     /locus_tag="OO515_000781"
     CDS             13253..14230
                     /gene="pspF"
                     /locus_tag="OO515_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6574793.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="OO515_000782"
                     /pseudo
     CDS             complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="OO515_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="OO515_000783"
     CDS             complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="OO515_000783"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6574794.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="OO515_000784"
                     /pseudo
     CDS             complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="OO515_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="OO515_000785"
     CDS             complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="OO515_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6574795.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="OO515_000786"
     CDS             complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="OO515_000786"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6574796.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20133..21494
                     /gene="puuA"
                     /locus_tag="OO515_000787"
     CDS             20133..21494
                     /gene="puuA"
                     /locus_tag="OO515_000787"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6574797.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21643..21807
                     /locus_tag="OO515_000788"
     CDS             21643..21807
                     /locus_tag="OO515_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574798.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21797..23182
                     /gene="puuP"
                     /locus_tag="OO515_000789"
     CDS             21797..23182
                     /gene="puuP"
                     /locus_tag="OO515_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6574799.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23316..23561
                     /locus_tag="OO515_000790"
     CDS             23316..23561
                     /locus_tag="OO515_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6574800.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23874..25517
                     /gene="sapA"
                     /locus_tag="OO515_000791"
     CDS             23874..25517
                     /gene="sapA"
                     /locus_tag="OO515_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6574801.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25514..26479
                     /gene="sapB"
                     /locus_tag="OO515_000792"
     CDS             25514..26479
                     /gene="sapB"
                     /locus_tag="OO515_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6574802.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26466..27356
                     /gene="sapC"
                     /locus_tag="OO515_000793"
     CDS             26466..27356
                     /gene="sapC"
                     /locus_tag="OO515_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6574803.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27356..28348
                     /gene="sapD"
                     /locus_tag="OO515_000794"
     CDS             27356..28348
                     /gene="sapD"
                     /locus_tag="OO515_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6574804.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28350..29156
                     /gene="sapF"
                     /locus_tag="OO515_000795"
     CDS             28350..29156
                     /gene="sapF"
                     /locus_tag="OO515_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6574805.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29224..29577
                     /locus_tag="OO515_000796"
     CDS             29224..29577
                     /locus_tag="OO515_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6574806.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29945..30733
                     /gene="fabI"
                     /locus_tag="OO515_000797"
     CDS             29945..30733
                     /gene="fabI"
                     /locus_tag="OO515_000797"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6574807.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30878..32004
                     /locus_tag="OO515_000798"
                     /pseudo
     CDS             30878..32004
                     /locus_tag="OO515_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32072..34006
                     /gene="rnb"
                     /locus_tag="OO515_000799"
     CDS             32072..34006
                     /gene="rnb"
                     /locus_tag="OO515_000799"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6574808.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34084..34187)
                     /locus_tag="OO515_000800"
                     /pseudo
     CDS             complement(34084..34187)
                     /locus_tag="OO515_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34241..36231
                     /gene="pdeR"
                     /locus_tag="OO515_000801"
                     /pseudo
     CDS             34241..36231
                     /gene="pdeR"
                     /locus_tag="OO515_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36378..36551
                     /locus_tag="OO515_000802"
     CDS             36378..36551
                     /locus_tag="OO515_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574809.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36641..37390
                     /locus_tag="OO515_000803"
     CDS             36641..37390
                     /locus_tag="OO515_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6574810.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37659..37877
                     /gene="osmB"
                     /locus_tag="OO515_000804"
     CDS             37659..37877
                     /gene="osmB"
                     /locus_tag="OO515_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6574811.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="OO515_000805"
     CDS             complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="OO515_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6574812.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="OO515_000806"
     CDS             complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="OO515_000806"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6574813.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="OO515_000807"
     CDS             complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="OO515_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6574814.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="OO515_000808"
     CDS             complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="OO515_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6574815.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="OO515_000809"
     CDS             complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="OO515_000809"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6574816.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41826..42416
                     /gene="ribA"
                     /locus_tag="OO515_000810"
     CDS             41826..42416
                     /gene="ribA"
                     /locus_tag="OO515_000810"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6574817.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="OO515_000811"
     CDS             complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="OO515_000811"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6574818.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45319..45414)
                     /locus_tag="OO515_000812"
     CDS             complement(45319..45414)
                     /locus_tag="OO515_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574819.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45528..45695)
                     /locus_tag="OO515_000813"
     CDS             complement(45528..45695)
                     /locus_tag="OO515_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574820.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45698..45862)
                     /locus_tag="OO515_000814"
     CDS             complement(45698..45862)
                     /locus_tag="OO515_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6574821.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10845 a        12142 c        11561 g        11374 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctccccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7081 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7141 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7201 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7261 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7321 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7381 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7441 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7501 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7561 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7621 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7681 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7741 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7801 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7861 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7921 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7981 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8041 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8101 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8161 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8221 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8281 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8341 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8401 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8461 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8521 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8581 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8641 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8701 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8761 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8821 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     8881 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8941 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9001 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9061 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9121 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9181 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9241 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9301 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9361 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9421 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     9481 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9541 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     9601 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9661 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9721 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9781 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9841 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9901 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9961 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10021 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10081 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10141 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10201 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10261 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10321 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10381 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10441 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10501 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10561 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10621 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10681 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10741 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10801 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10861 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10921 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10981 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11041 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11101 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11161 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11221 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11281 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11341 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11401 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11461 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11521 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11581 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11641 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11701 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11761 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11821 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11881 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    11941 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12001 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12061 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12121 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12181 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12241 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12301 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12361 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12421 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12481 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12541 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12601 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12661 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12721 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12781 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12841 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12901 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12961 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13021 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13081 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13141 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13201 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13261 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13321 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13381 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13441 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13501 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13561 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13621 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13681 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13741 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13801 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13861 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13921 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13981 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14041 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14101 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14161 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14221 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14281 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14341 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14401 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14461 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14521 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14581 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14641 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14701 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14761 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14821 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14881 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14941 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15001 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15061 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15121 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15181 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15241 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15301 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15361 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15421 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15481 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15541 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15601 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15661 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15721 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15781 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15841 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15901 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15961 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16021 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16081 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16141 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16201 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16261 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16321 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16381 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16441 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16501 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16561 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16621 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16681 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16741 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16801 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16861 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16921 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16981 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17041 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17101 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17161 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17221 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17281 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17341 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17401 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17461 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17521 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17581 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17641 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17701 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17761 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17821 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17881 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17941 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18001 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18061 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18121 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18181 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18241 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    18301 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18361 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18421 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18481 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18541 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18601 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18661 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18721 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18781 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18841 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18901 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18961 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19021 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19081 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19141 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19201 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19261 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19321 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19381 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19441 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19501 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19561 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19621 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19681 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19741 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19801 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19861 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19921 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19981 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20041 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20101 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20161 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20221 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20281 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20341 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20401 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20461 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20521 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20581 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20641 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20701 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20761 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20821 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20881 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20941 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21001 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21061 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21121 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21181 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21241 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21301 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21361 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21421 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21481 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21541 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21601 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21661 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21721 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21781 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21841 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21901 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21961 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22021 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22081 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22141 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22201 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22261 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22321 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22381 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22441 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22501 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22561 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22621 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22681 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22741 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22801 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22861 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22921 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22981 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23041 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23101 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23161 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23221 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    23281 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23341 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23401 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23461 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23521 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23581 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23641 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23701 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23761 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23821 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23881 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23941 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24001 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24061 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24121 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24181 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24241 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24301 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24361 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24421 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24481 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24541 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24601 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24661 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24721 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24781 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24841 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24901 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24961 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25021 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25081 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25141 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    25201 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25261 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25321 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25381 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25441 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25501 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25561 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25621 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25681 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25741 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25801 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25861 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25921 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25981 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26041 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26101 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26161 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26221 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26281 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26341 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26401 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26461 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26521 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26581 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26641 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26701 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26761 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26821 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26881 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26941 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27001 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27061 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27121 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27181 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27241 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27301 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27361 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27421 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27481 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27541 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27601 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27661 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27721 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27781 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27841 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27901 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27961 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28021 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28081 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28141 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28201 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28261 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28321 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28381 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28441 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28501 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28561 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28621 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28681 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28741 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28801 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28861 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28921 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28981 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29041 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29101 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29161 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29221 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29281 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29341 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29401 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29461 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29521 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29581 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29641 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29701 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29761 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29821 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29881 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29941 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30001 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30061 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30121 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30181 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30241 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30301 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30361 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30421 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30481 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30541 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30601 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30661 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30721 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30781 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30841 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30901 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30961 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31021 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31081 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31141 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31201 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31261 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31321 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31381 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31441 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31501 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31561 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31621 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31681 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31741 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31801 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31861 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31921 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31981 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32041 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32101 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32161 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    32221 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32281 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32341 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32401 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32461 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32521 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32581 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32641 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32701 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32761 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32821 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32881 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32941 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33001 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33061 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33121 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33181 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33241 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33301 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33361 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33421 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33481 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33541 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33601 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33661 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33721 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33781 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33841 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33901 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33961 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34021 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34081 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34141 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34201 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34261 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34321 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34381 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34441 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34501 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34561 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34621 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34681 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34741 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34801 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34861 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34921 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34981 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    35041 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35101 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35161 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35221 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35281 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35341 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35401 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35461 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35521 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35581 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35641 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35701 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35761 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35821 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35881 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35941 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36001 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36061 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36121 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36181 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36241 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36301 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36361 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36421 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36481 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36541 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36601 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36661 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36721 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36781 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36841 gagcaactat acgctgaagc gtgaactcgc cgagttcgcc gcgtcactgg ttcaaccggg
    36901 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36961 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37021 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37081 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37141 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37201 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37261 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37321 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37381 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37441 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37501 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37561 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37621 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37681 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37741 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37801 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37861 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37921 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37981 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38041 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38101 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38161 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38221 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38281 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38341 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38401 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38461 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38521 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38581 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38641 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38701 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38761 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38821 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38881 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38941 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39001 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39061 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39121 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39181 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39241 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39301 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39361 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39421 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39481 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39541 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39601 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39661 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39721 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39781 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39841 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39901 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39961 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40021 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40081 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40141 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40201 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40261 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40321 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40381 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40441 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40501 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40561 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40621 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40681 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40741 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40801 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40861 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40921 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40981 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41041 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41101 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41161 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41221 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41281 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41341 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41401 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    41461 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41521 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41581 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41641 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41701 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41761 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41821 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41881 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41941 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42001 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42061 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42121 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42181 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42241 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42301 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42361 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42421 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42481 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42541 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42601 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42661 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42721 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42781 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42841 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42901 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42961 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43021 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43081 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43141 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43201 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43261 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43321 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43381 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43441 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43501 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43561 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43621 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43681 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43741 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43801 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43861 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43921 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43981 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44041 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44101 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44161 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44221 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44281 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44341 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44401 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44461 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44521 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44581 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44641 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44701 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44761 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44821 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44881 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44941 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45001 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45061 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45121 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45181 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45241 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45301 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45361 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45421 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45481 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45541 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45601 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45661 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45721 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45781 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45841 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45901 caaatcctaa agaaacttta gt
//
LOCUS       DAJTXQ010000015        44615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000015 DAJTXQ010000000
VERSION     DAJTXQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.015"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2319
                     /gene="torZ"
                     /locus_tag="OO515_000815"
     CDS             <1..2319
                     /gene="torZ"
                     /locus_tag="OO515_000815"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6574822.1"
                     /translation="ILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHT
                     TTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIF
                     AGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQ
                     TSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSE
                     TIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGK
                     SDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTL
                     AAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPV
                     ARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVV
                     SECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVF
                     ADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEM
                     RRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEW
                     KGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIANGDL
                     VRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLA
                     NACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(2484..3455)
                     /gene="cmoB"
                     /locus_tag="OO515_000816"
     CDS             complement(2484..3455)
                     /gene="cmoB"
                     /locus_tag="OO515_000816"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6574823.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3452..4195)
                     /gene="cmoA"
                     /locus_tag="OO515_000817"
     CDS             complement(3452..4195)
                     /gene="cmoA"
                     /locus_tag="OO515_000817"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6574824.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(4236..4631)
                     /locus_tag="OO515_000818"
     CDS             complement(4236..4631)
                     /locus_tag="OO515_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6574825.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(4684..4923)
                     /locus_tag="OO515_000819"
                     /pseudo
     CDS             complement(4684..4923)
                     /locus_tag="OO515_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5173..5703)
                     /locus_tag="OO515_000820"
     CDS             complement(5173..5703)
                     /locus_tag="OO515_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574826.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            5894..6142
                     /locus_tag="OO515_000821"
     CDS             5894..6142
                     /locus_tag="OO515_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6574827.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            6144..8234
                     /locus_tag="OO515_000822"
     CDS             6144..8234
                     /locus_tag="OO515_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6574828.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            8317..9249
                     /locus_tag="OO515_000823"
     CDS             8317..9249
                     /locus_tag="OO515_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6574829.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            9252..9473
                     /locus_tag="OO515_000824"
     CDS             9252..9473
                     /locus_tag="OO515_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574830.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            9486..9740
                     /locus_tag="OO515_000825"
     CDS             9486..9740
                     /locus_tag="OO515_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574831.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            9742..10023
                     /locus_tag="OO515_000826"
     CDS             9742..10023
                     /locus_tag="OO515_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCR6574832.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            10020..10292
                     /locus_tag="OO515_000827"
     CDS             10020..10292
                     /locus_tag="OO515_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574833.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            10297..10590
                     /locus_tag="OO515_000828"
     CDS             10297..10590
                     /locus_tag="OO515_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574834.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            10602..11132
                     /locus_tag="OO515_000829"
     CDS             10602..11132
                     /locus_tag="OO515_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCR6574835.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            11278..11811
                     /locus_tag="OO515_000830"
     CDS             11278..11811
                     /locus_tag="OO515_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574836.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            11811..12326
                     /locus_tag="OO515_000831"
     CDS             11811..12326
                     /locus_tag="OO515_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574837.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFYFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            12330..12881
                     /locus_tag="OO515_000832"
     CDS             12330..12881
                     /locus_tag="OO515_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCR6574838.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTHISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            12878..13207
                     /locus_tag="OO515_000833"
     CDS             12878..13207
                     /locus_tag="OO515_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574839.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAAVLIVQYGHRQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREVNA"
     gene            13204..13554
                     /locus_tag="OO515_000834"
     CDS             13204..13554
                     /locus_tag="OO515_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574840.1"
                     /translation="MRSYIDNEKLETISDCLSLLAKIKETIEEIKFQLEYAPCGDDAW
                     RNAARKALAVFQKQRRAVEYRLAVLRQEEKERNIRRHERVNDFLVRELKERVPESVFF
                     ECEAIARSKALETD"
     gene            13570..13878
                     /locus_tag="OO515_000835"
     CDS             13570..13878
                     /locus_tag="OO515_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574841.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKFVSFMDKECDKRDMPEI
                     IMDDMILDSAVALSPLWIVHTLAEIAKRSDKQAAVAALRLLAELSCSVNPNTFFDEPG
                     "
     gene            complement(13840..14250)
                     /locus_tag="OO515_000836"
     CDS             complement(13840..14250)
                     /locus_tag="OO515_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCR6574842.1"
                     /translation="MKIDYDEVLSILTTFQDAETPFLTLQDLGMAEAEGEEKDKKVFH
                     LMLLADNGSIVNGDMQSETPKYIGFFFHSLGVGFRNTPIMLTQQGHDLANALRKKPIL
                     ERIKKEFTDAPFDLIKEVTKSMLTRFVKERIGID"
     gene            14315..14827
                     /locus_tag="OO515_000837"
     CDS             14315..14827
                     /locus_tag="OO515_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCR6574843.1"
                     /translation="MNRASLITLIHVAKRDLQLDHETYTSVLLAATGKPSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            14898..15320
                     /locus_tag="OO515_000838"
     CDS             14898..15320
                     /locus_tag="OO515_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6574844.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            15392..15892
                     /locus_tag="OO515_000839"
     CDS             15392..15892
                     /locus_tag="OO515_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6574845.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            15927..16355
                     /locus_tag="OO515_000840"
     CDS             15927..16355
                     /locus_tag="OO515_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574846.1"
                     /translation="MCRCAAAGCAVSAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAAAVPVPDDVADRVRRAVCEIRAGEAGADPR"
     gene            16339..16557
                     /locus_tag="OO515_000841"
     CDS             16339..16557
                     /locus_tag="OO515_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574847.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            16568..16795
                     /locus_tag="OO515_000842"
     CDS             16568..16795
                     /locus_tag="OO515_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCR6574848.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            16776..17087
                     /locus_tag="OO515_000843"
     CDS             16776..17087
                     /locus_tag="OO515_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCR6574849.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            17080..17370
                     /locus_tag="OO515_000844"
     CDS             17080..17370
                     /locus_tag="OO515_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6574850.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            17373..17954
                     /locus_tag="OO515_000845"
     CDS             17373..17954
                     /locus_tag="OO515_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCR6574851.1"
                     /translation="MDKPARGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            17965..18213
                     /locus_tag="OO515_000846"
     CDS             17965..18213
                     /locus_tag="OO515_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574852.1"
                     /translation="MATDNRSLMSSAHQKAFSLATQLPPETIKVCRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            18213..19877
                     /locus_tag="OO515_000847"
     CDS             18213..19877
                     /locus_tag="OO515_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574853.1"
                     /translation="MNKARETSPSLMQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALQERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            19877..21466
                     /locus_tag="OO515_000848"
     CDS             19877..21466
                     /locus_tag="OO515_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCR6574854.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHSGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDDTGD"
     gene            21450..22781
                     /locus_tag="OO515_000849"
     CDS             21450..22781
                     /locus_tag="OO515_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCR6574855.1"
                     /translation="MTQGIDLGYAATLPSKEAVAYFRVKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(22783..22862)
                     /locus_tag="OO515_000850"
     tRNA            complement(22783..22862)
                     /locus_tag="OO515_000850"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            22903..23376
                     /locus_tag="OO515_000851"
     CDS             22903..23376
                     /locus_tag="OO515_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6574856.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            23554..24678
                     /locus_tag="OO515_000852"
     CDS             23554..24678
                     /locus_tag="OO515_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCR6574857.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            24678..25625
                     /locus_tag="OO515_000853"
     CDS             24678..25625
                     /locus_tag="OO515_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCR6574858.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            25669..26022
                     /locus_tag="OO515_000854"
     CDS             25669..26022
                     /locus_tag="OO515_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574859.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            26019..26438
                     /locus_tag="OO515_000855"
     CDS             26019..26438
                     /locus_tag="OO515_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCR6574860.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDARKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            26435..26998
                     /locus_tag="OO515_000856"
     CDS             26435..26998
                     /locus_tag="OO515_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCR6574861.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLISAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHISLPGATAAGEKAGPSEE"
     gene            27002..27289
                     /locus_tag="OO515_000857"
     CDS             27002..27289
                     /locus_tag="OO515_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6574862.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMKPLAQEGEEKPFTPFWCRRLNDGD
                     VIVVEKAAEAAPAAASATTDAAGKPAGAAAEKPATEKPATEKPAASDKGTS"
     gene            27289..28791
                     /locus_tag="OO515_000858"
     CDS             27289..28791
                     /locus_tag="OO515_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6574863.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGVGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADNGTPVRPGQNIVTPEIMTLQLIALGEEWV
                     ELGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            28800..29165
                     /locus_tag="OO515_000859"
     CDS             28800..29165
                     /locus_tag="OO515_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6574864.1"
                     /translation="MSGKQHQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            29180..29659
                     /locus_tag="OO515_000860"
     CDS             29180..29659
                     /locus_tag="OO515_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6574865.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDVLEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLIASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            29787..31844
                     /locus_tag="OO515_000861"
     CDS             29787..31844
                     /locus_tag="OO515_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6574866.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSEAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFARDVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            31831..33189
                     /locus_tag="OO515_000862"
     CDS             31831..33189
                     /locus_tag="OO515_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6574867.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSMAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            33173..34297
                     /locus_tag="OO515_000863"
     CDS             33173..34297
                     /locus_tag="OO515_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6574868.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRSKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            34287..34901
                     /locus_tag="OO515_000864"
     CDS             34287..34901
                     /locus_tag="OO515_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6574869.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            34894..35331
                     /locus_tag="OO515_000865"
     CDS             34894..35331
                     /locus_tag="OO515_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6574870.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            35331..36413
                     /locus_tag="OO515_000866"
     CDS             35331..36413
                     /locus_tag="OO515_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6574871.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            36404..36964
                     /locus_tag="OO515_000867"
     CDS             36404..36964
                     /locus_tag="OO515_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6574872.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            36964..37854
                     /locus_tag="OO515_000868"
     CDS             36964..37854
                     /locus_tag="OO515_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6574873.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLATPRNINGVPFDGTQD
                     INITSGITQQDADSRYIQDIRLGAENRQVMYGGAYYSAYGNVMAGLQIDSKVDASNDF
                     IAFRPLQKLVGGTWITVSQL"
     gene            37906..38427
                     /locus_tag="OO515_000869"
     CDS             37906..38427
                     /locus_tag="OO515_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6574874.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
     gene            complement(38459..38641)
                     /locus_tag="OO515_000870"
     CDS             complement(38459..38641)
                     /locus_tag="OO515_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6574875.1"
                     /translation="MTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYYR
                     PLQYLLNGAWVTAASI"
     gene            38889..39461
                     /locus_tag="OO515_000871"
     CDS             38889..39461
                     /locus_tag="OO515_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6574876.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGCHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPASPAPSPKVP"
     gene            39547..41601
                     /locus_tag="OO515_000872"
     CDS             39547..41601
                     /locus_tag="OO515_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6574877.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            41746..41928
                     /locus_tag="OO515_000873"
     CDS             41746..41928
                     /locus_tag="OO515_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR6574878.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            41964..42218
                     /locus_tag="OO515_000874"
     CDS             41964..42218
                     /locus_tag="OO515_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR6574879.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(42386..42646)
                     /locus_tag="OO515_000875"
     CDS             complement(42386..42646)
                     /locus_tag="OO515_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574880.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            42936..43136
                     /locus_tag="OO515_000876"
     CDS             42936..43136
                     /locus_tag="OO515_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6574881.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            43090..43827
                     /locus_tag="OO515_000877"
     CDS             43090..43827
                     /locus_tag="OO515_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6574882.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRTNIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT        11007 a        11149 c        12546 g         9913 t
ORIGIN      
        1 atcctcaccg cggaacgttg gggagcgatg aacgtagaag tgaaggacgg caagattgtt
       61 tcttcaacag gcgcgctggc gaaaaccata ccgaattcct tacagtctac ggcggcggat
      121 caggtacaca ccacgacgcg tattcagcat ccgatggtga gaaaaagcta tctcgataat
      181 ccactgcaac cgacgaaagg tcgtggcgaa gatacctatg tacaggtgag ctgggaacaa
      241 gcattaaaac tgattcatga gcaacacgat cgcattcgca aagctaacgg accatcggct
      301 atttttgccg gatcatacgg ctggcgctct tctggcgtgt tgcataaagc gcaaacctta
      361 ctgcaacgtt atatgaacct ggcgggcggt tattccgggc atagcggcga ttattcaacc
      421 ggggcggcac aggtgatcat gccgcatgtg gtcggttctg tagaggtgta tgaacagcag
      481 acctcctggc cgctgattct ggaaaacagc caggttgtgg tgctgtgggg gatgaacccg
      541 ctgaatacct taaaaattgc ctggagcagt accgatgagc aagggctgga atacttccat
      601 cagctgaaaa aatctggcaa accagtgatt gccattgatc ctatccgctc cgaaaccatc
      661 gacttctttg gcgataatgc cacctggatc gcgccgaata tgggcaccga cgtggcactg
      721 atgttaggga ttgcgcatac cctgatgaca caaggcaaac acgataaagt gttccttgaa
      781 aaatacacta ccgggtatcc gcagtttgaa gagtatctga gaggtaagag cgataatacg
      841 ccgaagagcg cggcctgggc ggcggaaatt actggcgtac cggaagcgca gatagtcaaa
      901 ctcgctgaac tgatggcggc gaatcgcaca atgctgatgg caggctgggg aattcagcgc
      961 cagcaatatg gtgaacagaa acactggatg ctggtgacgc tggcggcgat gttagggcag
     1021 attggtacac cgggcggcgg ttttggtttc tcttatcatt actccaatgg tggcaacccg
     1081 acacgtgttg gcggcgtgct gccggaaatg tctgcggcga ttgccggaca ggccagcgag
     1141 gccgctgatg atggcggaat gagggcgatt cctgttgccc gtatcgtcga tgcgctggaa
     1201 aatcctggcg gcaaatatca gcataacggt aaagagcaaa cgtatccgaa tatcaaaatg
     1261 atctggtggg cgggtggcgg taactttacc catcaccagg atactaatcg tctgattaaa
     1321 gcatggcaga aaccggagat gatcgtcgtt tctgaatgct actggaccgc agctgctaaa
     1381 cacgcagata tcgtattacc gatcaccaca tcgtttgagc gcaatgactt gacgatgacc
     1441 ggtgattaca gcaaccagca tattgtgccg atgaagcagg ctgttgctcc gcaatttgaa
     1501 gcgcgaaacg attttgatgt gtttgccgat cttgcggaat tactcaaacc tggcggaaaa
     1561 gagatctata ccgaaggtaa agatgaaatg gcgtggctga aatttttcta tgatgccgct
     1621 cagaaagggg cccgtgcgca acgcgtcact atgccaatgt ttaatgcctt ctggcagcaa
     1681 aataaactga tcgaaatgcg ccgcagcgag aagaacgaac agtacgttcg ttatggtgat
     1741 ttccgcgccg atccggtgaa aaatgcgctg ggtacgccaa gtggcaaaat tgagatttac
     1801 tccaaaacgc tggaaaaatt tggctataag gattgcccgg cacacccaac ctggcttgcg
     1861 cctgatgagt ggaagggtac cgccgacgag aagcagttgc agcttctgac cgcacatccg
     1921 gcacaccgtt tacacagtca gcttaactat gcggaactgc gtaaaaaata tgcggttgcc
     1981 gatcgtgaac cgatcactat tcacaccgaa gatgctgctc gatttagtat tgcgaatggc
     2041 gatctggtgc gtgtctggaa caaacgtggt cagattttga caggcgcggt ggtgactgac
     2101 gggatcaaaa aaggcgtggt atgcgtgcat gaaggtgcat ggccagatct ggaaaacggc
     2161 ttgtgtaaaa acggcagtgc gaacgtgtta acggcggata tccccagctc gcagctggca
     2221 aatgcctgtg ccggtaactc tgcgctggtg tatatcgaaa aatatacggg taacgcaccg
     2281 aagttaacgg catttgataa gcctgctgtt caggcgtaag aagtgaatag cgcccggaaa
     2341 cgggcgctga ggttatgcaa acgtaaaact gcctgatgcg ctatgcttat caggcctaca
     2401 tagtccctgc aatagattga attagcaagg gtttgtaggc cggataaggc attcacgccg
     2461 catccggcag taataccaga cctttacggc ttgcgcgcaa tcagcaccgc gcgtttaggc
     2521 gcaggataac cttcaactgt cttactcgga tcatgtgggt cgaggaaatc ggacagagat
     2581 tcggtaacca tccattcagt gcgtcgctgc tcttcagtag tggtaacgca cacatccaca
     2641 atgcggatat ctacaaaacc acacttcttc agccagtttt tcagcgccag cgcggaaggg
     2701 atgaaataga cattacgcat ttgcgcgtaa cgatcgcctg gtaccagcac cgtgttctca
     2761 tcgccatcaa taaccagcgt ttccagcacc agttcaccct cattcaccag ttgatctttc
     2821 aactgccaga gatgctccag cggcgaacgg cgatggtaaa gcacgcccat cgaaaagacg
     2881 gtatcaaagg ctttcagtgc cggaagctgt tcaataccta acggtaacaa atgtgcgcgc
     2941 tgatcgttac ccagtagttt acgcaccgct tcaaactggc acagaaacag ttgcgtaggg
     3001 tcgatcccca ccgcgaggtg cgcgcctgcg ccaatcatgc gccacatgtg ataaccgctg
     3061 ccgcagccga catccagaat ggtgcgtccg gttaaatcag aaagatgggg cagaacacga
     3121 tcccatttcc agtcggaacg ccattcggta tcgatgttga cgccatacag tgagaacggc
     3181 cctttgcgcc atggcatcag gttccgcata agcgtttcaa tgcgtttaat ttgcccggtg
     3241 ctcagtggct cttcgctttc ggcggttacg ctatgcaata aatccagacg atacggttta
     3301 atttcaggca gaaattccac cgcgttggac cactgcttaa acagcccgtg ctgctgctcg
     3361 cgctgccagt tagcaatctg cgcgggcagc gtttcgagcc agtgtgaaag atgatttttg
     3421 gcaatcagag aataaaagtt accaaagtcg atcatgcagc gtcctctgct tttaatgcca
     3481 ccagtgaacc aaagttaaag cactggaacc acagctcgct atgctcaaaa ccggctttat
     3541 gcaggcgtgc tttatgggtt tccacggaat cggtcagcat cacgttttcc agcatgctgc
     3601 gcttctggct gatctccagt tcgctgtaac cgttggcacg tttaaagtcg tggtgcatgt
     3661 tgaacaacag ttcaccaact ttggcatctt cgaaactgaa tttttccgaa agcaccagcg
     3721 cgccgcccgg gttcagtcct tgataaattt tatccagtaa tgcctggcgc tcggaaggtt
     3781 ccaggaattg cagggtaaaa ttcagcacca ccatcgatgc gttttcaatg gcgatatcgc
     3841 gaatatcacc ttcaatgacg tctactggcg taggggcttt ataggcgtca atatgacgac
     3901 tgcagcgttc aatcatcgcc ggggagttgt cgatggcaat aattttgcaa ttatcatgat
     3961 gaatgttgcg acgcaccgag agcgtcgccg cgcccagaga acaacccaga tcgtaaacct
     4021 gcgtaccagg ttgaacgaag cgctcggcta acataccaat catggaaata atattggaat
     4081 agccgggaac ggaacgctgg atcatatccg ggaagacttc agctacccgt tcatcaaagg
     4141 tccagtcgcc cagtctggcg ataggggcag aaaatagcgt gtcgcggtga gacataacgt
     4201 aaaaatccgg gaaaaagaaa gtggcgtatt gtgcgctaac gcagggagaa aaccaactcc
     4261 cagggcatat accaaagatt cgccagcacc atcagcaaca gcgcacacca ggtggcgctc
     4321 atgccggaac gtcgccagcg aaacagacgg tgatgaaaac cgtaataatg catcagacga
     4381 ccagcaagca aaacgatgcc gcaaatatgc accatccagg tttctgcgcc attcatttcc
     4441 ataaacagca tcagcacaat cgcgatggga atatattcca ccgcgttacc atgaatgcga
     4501 atagcgcttt gcagttcgct aaaaccaccg tcaccatagg caacgcggta ctgcattcgc
     4561 aggcgaacga catcaaaaga gaacttcatt aataacaacg cacttaaaac ggcatatagc
     4621 gcgcttacca tacaaactcc ctttaaaatg gccgatggca cctgtctatg ataggtggca
     4681 aattcagaaa agagaagatt gctgtggaat agccggaact gctccgatct ctggaagcgt
     4741 tttacgtaag tcttcccaca gggtatgtac cagttccggg gcctgggcaa tgtctggcgt
     4801 atgtaaaaaa agataaggcg tagtggtctg atgccactgc gctaatttct gtaaccagac
     4861 ctgaaataat tcccggtttt gcgtcatatc atcactaccg ataaaacgaa tcagcggatt
     4921 tgtgtaacca ggtgaatcat ttgataccaa cgagggtttt agtgaaagga ggtgaaatca
     4981 cctgtaagat actgaaataa aacaggtgat gtctggatga gcgcttaaag tctatttgat
     5041 atcgattgtg caataattaa tcgtttattc taaaatcggc aaaacttttg atactgcgat
     5101 ctcaactttt taaagcgttt aaaacttcat taaatctata tggcaactaa catacgggcc
     5161 agcctcgcca aactaacccg ctttagattc taccggattt gattgcctac ttgtctcggt
     5221 tttttccaaa atctctctga cgcaatcgtc tggcatatct acaatcatca acgcagccag
     5281 gcgcttcctg ccttctagtt gtaataaatc ctggttttct ttattcagca gtgtcagaac
     5341 cgtctctaca cctttgcgag caaggagatt ggttaactgt tctcgttcgt ctttcgtaag
     5401 gaatgaaagc atctgtgtaa gctgatctcc cttatcacca gaacaaccaa aattaccata
     5461 aataccaata tcgtttggta attcaccctt gccctcttta agccactcaa gactaacgcc
     5521 ttcactttca gaaattcgta taaggatact ttctctgggg gctggttgtg tttgtcctcc
     5581 cttgaaatag ctgtttaatg tgttgatatt gattccccac gctctggcag ctgcacttct
     5641 tgaagggtag cgctttgtaa gcattccaat acgttcaata acaggttctt ttccctgcga
     5701 cataaagata cctcttggtg cttttctaag gaaaggtttc tttctattta tccaattgaa
     5761 aaataaggat aaaagcaaaa attagccaaa ataccaaaaa taccttgatt tggtattttt
     5821 ggtgatgcat tatttacccg tacagatatc cgcacgaata tccgcgcggg taacttttta
     5881 gaatagagga agtatgagta gaagagaagt ttcagatcac gattggccgc ctgaaatcat
     5941 caaggcgcgc ttacacatgg ctggcttatc actgcgctct ttatctttaa aggctggtta
     6001 cagcagggat tcgctgaaaa gcgtattacg tactccttgc cgaccgtatc agcaaataat
     6061 tgctgatgct cttggggtat cacctgaaga aatctggccc agtcgatacc aggttaagag
     6121 ctatatgaga aaggcgtcat gatatgtatg tcattgcaaa agaactgatt ggcgcgcccg
     6181 gaatgcctgc tacaacaaaa ggtattcgcc aggcattaca acgttacgta caagggaaaa
     6241 gctgttgttc ccgtcgtcgc tcaggctcta aagcaactga atacagcatc gactgtttac
     6301 ctgaagtgac gcagcaggca ttacgtgaac gctacgccct gcaactgatg acgcaaaaag
     6361 ccgatgaatc accggctccg gtggtgacaa aggccagacg ctcacctgcc gtggttgatg
     6421 cggtggaggc atatcgcgga tcaccccaac tgatggtcga acgcctcaat gccctgactg
     6481 aaaaccaacg taaagtggca gaggcacgaa tagcgatcgt cagcgaggtg ctgaaaatcg
     6541 cgcaacaacc cggctttagt tgcgctaagg ctatccggtt cctcgttaac agcctggcac
     6601 gatcacaact ggacgagcgc attgtggcaa tggttgagac ggcgaacgcc aaaaagggaa
     6661 acagccgcgc gttgagtgaa atcacgctga aacgctggat tgcggccttt aacaaggcac
     6721 agaacgccgc tgaacgcctg cttttactgg caccgggtaa acgccaggaa ataaaagccg
     6781 aagatattaa ctggctgccc gaatttctgg cgcagtatcg ccagtcaaac ggccgaccaa
     6841 tgaccgaggc ttacgaggat tttgttgctg aatggcagca ccggcacgct gatgagcctt
     6901 atatgctcga tatcatgccc tcttatgaca ccattcgccg cgcaatgaag aaactgccgg
     6961 aagtggtgaa acaaaaaggc cgggtaactg gcagtgaata ccgtcagctt gagggattca
     7021 cgcgccgcga ctggtccaga atgccggtga attatgtctg gattggtgac ggtcacggca
     7081 tgaagctgaa atgcgcgcac ccggttcacg ggcggccatt cgcaccggaa gtgacctttg
     7141 ttatcgacgg tggcacgcgc tttgtggtgg gctggagcct tgacctggct gaaaatgttt
     7201 tcgccgtagc cggtgccata cagcacggta ttcgccatca tggcaaaccg tttctgtatt
     7261 actcggataa tggctccggg gaaaccgccg acgtcctgga taaggagatt gtggggatac
     7321 tgccacgact ggggattaat cacccgacag ggattgccgg taatccgcag ggacggggca
     7381 ttatcgaacg gcttaaccgc acattaccga tgcgcatagc ccgtaaatac cgcacctatt
     7441 tcgggaaagg agcagatcgc gagacgttac gtaaaaccaa ccgcgattta cgctcggcat
     7501 tcagtgccct gcaacagggc aaacggctga acgcccggca gcagtcagcg atgcgtgatt
     7561 taccgtcctg gtctgaactg attgatgcca ttcgtgacgg tgttgagtgg tacaacaacc
     7621 ggccgcacga tgaattaccc atgaagccga acggtaaaca ttacagcccg gcggagttca
     7681 gaaaaaaacg cctggcggaa gaggacacgg aaattgaatg gctgtccgat gttgaactgc
     7741 gggatatgtt ccggccgatg gtggagcgcc ctgtaagacg ctgtgaaata cgctggctga
     7801 ataatattta ctacgcgccc gagctgcgtg acgagcatgg ccgtaaggta cttatcagct
     7861 atgacattca tgatgccgaa cgaattaccg tgcgccgccc ggatggcagc gtgatttgcg
     7921 aggcggtatg ggacggcaat aaacgcgaag ccttccctgt cagcgcggaa tactacaaac
     7981 agcagcagcg ccttaaaggc atgcgtaaac gcgcagagga aaaaatccgt gatgccgagg
     8041 atgaggttgt caacgtgctg gagcacaagc cgcaggagcc ctggctggaa aacatatacc
     8101 gccctgtggg taatacggtg accgttcagc aaccggtcgc tgacgatgaa cctgatgaag
     8161 aatacgagcg taatttccag cggggattgc aactgctcga agcgaaatta aaagaaagtg
     8221 atccgctggc ctgaaataaa aaaataaccc gaggggtgac tcaggttatt tgaggtttca
     8281 gtctcggaga atgctgtctc aagcgaggtg aataatatga ccgatattaa cgatgtaatc
     8341 aagaccattg atgaacttat tgatggcggc gtactgacgc agtatgccat cgccagagag
     8401 gcgggaattt ccgacggcac attatcggct ttccgtaagg gaaaatataa aggcgataac
     8461 gctgctgtgg ctgcttccct gcgctcctgg tatgagaact ggaataaaca aagcgcactg
     8521 ccggaaccgc cgcagtttgt ggaaactcag acagtccagg agctgcgcgc actgtttcag
     8581 gcggttcgcc tgatgggctg tattaacgtt attgtgggcg taccgggtgt gggtaaaacg
     8641 gccactgccc gtaattactg ccaggagcaa ccaaacacct ggatgatcac cctgtcaccc
     8701 gcgcactcca gcgtcacgga gtgtctgctg gagctggccg atgcgctggg gattgattac
     8761 acccgcgcga acaaaggggc attatcccgc gccatccgcc gtcgcctgat gggaacgcgt
     8821 ggactggtga ttgtggatga ggcggatcat cttggtattg acggtctgga gcaactccgg
     8881 gcaattcagg acgccacggg gatcgggatg gtgcttattg gtaacccgcg cggattgttt
     8941 aaaggtggac gccgcgcctt tgatgattta tcgcgcctgt tcagccgtct tgcccgtaca
     9001 aaacaacttc gcaaggccaa aaaggcggat gtgctggcca ttgccatggc gtgggggatc
     9061 agtggtgagg ccgagctggc cgtcatgcag gctatcgctg aaaagccggg agcgttacgc
     9121 gttctgacac atacgcttaa ccaggcgtgg ctcaccgcca gcggtgaagg cgcggcgctg
     9181 acagaaaaac atattaatgc ggcctttaaa gaggtttata ccaaccctga attactctca
     9241 caggtgtgat tatggcggta tttaatattc ctgatattta cggacgcttt tacctggtta
     9301 atttcgataa cgtgaaggtg atttcactgg ccgaaaataa agaatgtggc gatttacttt
     9361 ttgaatttaa tgaccgcaca cgaatggtga tatctgccgg acttgatcgc gaaggtgcga
     9421 cagaagttta cagcggaata tgccgttctg ttggtgcgaa acaagtcagc taaatgaggt
     9481 gttatatgaa tatgcaatcc tgcggtaaca aaatgaattt attcgactcc ctgaacagcg
     9541 cgcgccgtct gaccgaactt gccggtgcgg tactggaacg cagtaagcgc tatccgcaac
     9601 gttttgcact gaaaaccacg ccgccggtag gcaacgtgca gggaaccggt gaaattgaaa
     9661 tcaccataca gaccaacggc ctgcgccgcc gtgtgaaggc cacccgcatc agcggctgca
     9721 cggtttactg ggaggtgtga ggtgaaaaaa aatctcattg catgggcgtg gtcgagtggt
     9781 cttattgagt ttggttacgt cctgccggaa ggtgcattgc cgatagttgc cggaaagcct
     9841 gccacggtac ggcatgtgat tgaggttatg gcgcgtcatg gacgtgatga acaggagcag
     9901 ttactggttc cggggatacc ggaagcggtg acggaggaag aagcctttaa tgccatgatt
     9961 cggttctgcc gtgaggttag acgccgggtc agttatccaa acagaacgag gaccagagga
    10021 tgagtaaagt cgtacgcatt attttcgaat acaaggaaga tgttatctgc aaaaactctg
    10081 atggcagcac gcacagaggg gtaagtctgg atatacgttc aaccggaata aagaagaaag
    10141 ataatggacc cgccatgatt tttggggtgg ttatgctggc agaaagcaga aacttcgcag
    10201 agtttgtggc aatgaaagcc agtgcattca tgaaagacag aggcatggct tccggggtta
    10261 ttaacggtaa tgaatttaat caacaggggt aattccatga gcaaagtacg cgttattttt
    10321 gaatttaatc atgtttcgca tgacgaaaag ttggcaggca atgactgtgt tgaagtgcat
    10381 gaaaagattg gagtggatgt gaaaacagaa cgtgatacgg ataacaggcc gacgtcactc
    10441 tgtgacgttt atgcaagtat tctccagtat cacagtcctg caattattca gtttctctca
    10501 gcggaatttc aggcatctgc acagtctttt ggagcggatg ccatcattaa acgccaccgc
    10561 gtgcataaag catcaggcac actgcaataa ggaaaaacaa aatggctaaa cgcgttacaa
    10621 aattaaaggc cgcagccgag gcggcaccgc agacccggga agaagtcagc cgtgatatcc
    10681 gcaccctggg agatattcag cgagaggcgc tgcgcctgga aacggcgatg aatgacgaag
    10741 tggcagaaat caccgcccgt tatacgccgc agattgaaaa tcttaaaaag caaatcaaag
    10801 tgctttttaa agggattcag gactggtgca aaaccaaccg tgatgagctg acgaacggcg
    10861 gcaaaaccaa aacagccaat ctgaccaccg gaacggtgtc atggcggctg ggaacgccat
    10921 catgcagcgt cagccgtgat gtggaaggtg tgattgaaat gctgcgccgt atggggcttg
    10981 agcgtttcat ccgcacgaaa gaggaagtga acaaggaagc cgtcctggcg gagccggatg
    11041 ctgtgaaagg cattgccggt atcaaggtga ataaaggcgc tgaaagtttt tatgtcgagc
    11101 cttttgaaca ggacgccgga ctgaataaat aacaccgcat taatcaatta aatatcactt
    11161 cattttaatt atggcgctcg cgtcagggga ctgcttgcgc ctgaaaacag aaaataaggg
    11221 ttaagaaata tgaaatacgt ttatatcgtc attaataatg tggtggggaa ataaatcatg
    11281 attgatgcaa aagtgcttga aggggttaaa aactggctgt gtatttatgg ccgcctgacc
    11341 tgcggtgtac tggctgaaaa aatgaatatg ccgccatcct ccatggttta ttttttgcgt
    11401 gatgcggttg atgcaggcgt gctgacggaa tgtaacggtt tttatgatat tccgcgtccc
    11461 cgcccggtgc agccggttcg tcgcaaatgc agccaggaat ctgcggctga tgatgttcag
    11521 tggtgcggct tcagaaaatc cctgccatgg attgaggggc atgatattcc gtcgatggcg
    11581 tgggaatttg ctcagggcgt tctgacctgt gaaaccgttt atgtggtggc tgaagttgat
    11641 gagcaggcca tgaaagaagg cgtgccccag tttgtgatgg cgtatatcga catacgcctg
    11701 ggcgtcatta tctgcggttt aagcggctgg aatatcactg aacatgttct gcgctacctg
    11761 attgttgacc ggacagcagc gcctgccggg atatctgcgg aggtggcgta atgttcttta
    11821 aaacatcaaa cccttccgcg ctggctgcgt ggcaaaaata ccagcaggac tgccagaaag
    11881 ttaaggatga ggcaaaacgc cttgaggccg tgctgaatgt tgcgtgccgg tcggtatttg
    11941 tatccggtat cagtggattt tactttaagg gactgcgttt tacggaggac aaatatcctt
    12001 ttcatcgcga cttatggcga aaaccgactg cgtcgaatgg ctggagctgc acaccgcgca
    12061 catcacgtat ccctaaaacc ctgcgtgttg cctctgatga actgaacagt ctgtggcgtg
    12121 aatattcgcc cgtcacgtat gccagaaccg atgcgctgtt gttctggttg ggtattgatt
    12181 tctcggcaat cctgtttggc cctgtgaagt ggttctgcgt tgacgatgtg atttaccttc
    12241 agtgcggcgt aaaacccgca aaacagaaaa tgaccgaaat actgtctgat gagttttatg
    12301 ctgctgaaaa gcgagtcagg gggtgatgca tgatgaaatt acaacccatg ggactaaaag
    12361 gtcgcgcccc cgctcatgtc cgtccgtgga cacctgaaga agatgcgctg ctgattgcgc
    12421 tttattcatc caccccggtt aaggatattg ctgccagaat aaaaagaact gtctgggctg
    12481 tatataaccg gactggtgta ttgcgcagtt catacccgga gttactgaaa tataaacacc
    12541 caagatttac acctgatgaa gataagttta ttcgaaaaaa tgccagaaca atgacctgcc
    12601 agcaaatggg agaatatctc ggacgtaata aagattctgt cagatgtcgg gcaggaatga
    12661 ttggtgctgg attaacaaag tgcggagagt tacgccccgg aacgcacata tctgatgatg
    12721 atgtacgtct tatacgtgcg ctgcgtgatt ccgattaccc acgccgtctg tcattccggg
    12781 aaattggcga aaagtttgga atatctgaac attctgctca cgcagtttat taccgtcgcc
    12841 ggactgccga ggacgctgta ttacgggagt taacgccatg ataacgacct tatttgttga
    12901 atcagatgaa cctcttgtgt gtgccgccgg aatgccgctc tgtggcggaa cgctgaccgg
    12961 tgtttatttc ggggatttac gcggttatcc ctggcattca ctgaatgatg ctttcccacc
    13021 tgatatggag gctgccgtgc tgattgttca gtatggtcac cgtcaggagc tgcgcatcgg
    13081 ccatatgggg tatgaaggct tttttgttga tgaagaaacc ggagcctgcc ttgaagatga
    13141 agacggacag gtgacgcact ggtgtcatat ttcggcttta ccggaattac gggaggtgaa
    13201 tgcgtgagaa gttatattga taatgaaaag ctggagacaa taagcgactg tcttagcttg
    13261 ctggcaaaaa tcaaagaaac tattgaagag attaaatttc agcttgaata cgcaccatgt
    13321 ggcgatgatg catggcgtaa tgctgccaga aaagcactgg ctgtctttca gaaacaacgc
    13381 cgggctgttg aataccgcct tgctgtattg cgccaggagg aaaaggaaag aaatataaga
    13441 cgtcatgaac gcgttaatga tttccttgtt cgtgaattaa aagagcgcgt gccggagtcg
    13501 gtgttttttg aatgtgaggc tatagcccgt agcaaagcac tggaaacaga ttaagcaggc
    13561 aggtgaataa tgagtattga attaagatcg tcttatgaat atcgcaaaat ccttatcgcc
    13621 ggaggcatga aaccggaaga tgcagagaaa ttcgtttctt ttatggataa agaatgtgac
    13681 aagcgggata tgccagaaat tattatggat gacatgattc tggattcagc cgtggcgtta
    13741 agtccgttat ggattgtgca tactcttgcg gaaatagcta aaagaagtga caagcaggcg
    13801 gctgtcgccg ccctgcgatt actggctgaa ttaagttgtt cagtcaatcc caatacgttc
    13861 tttgacgaac ctggttaaca ttgatttggt aacttcttta attagatcaa atggtgcgtc
    13921 tgtaaactcc tttttgattc tttcaaggat tggttttttt ctcagggcgt tagctaaatc
    13981 atgcccctgc tgggtaagca taatgggcgt gtttctgaaa ccaacgccca gagaatggaa
    14041 aaagaaacca atatatttgg gtgtttctga ctgcatatca ccattaacaa ttgagccatt
    14101 atcagcgaga agcataagat gaaaaacttt tttatccttt tcctctcctt ctgcctcagc
    14161 catgcccaga tcctgtagtg taaggaaagg tgtttcagca tcctgaaaag ttgtgagaat
    14221 acttaatact tcatcgtaat ctattttcat atcgatacct ttagttttat ccgggcggcg
    14281 ttgccatgcc gcctgttgtt acggagacac gataatgaat cgcgcttccc tgattacctt
    14341 aatacatgtc gcaaaacgcg atctacagct tgaccatgag acctacacat ccgtactact
    14401 ggcagccacc ggcaaaccca gttgccgtga tatgtcaccc ggtgagttat cccgcgtgct
    14461 ggatgttttc aaaaaacgcg gttttaaagt gcgtcagaag ccggttaacc gggccttaaa
    14521 accgggtacg gtgaccgcca aaattcgcgc catctggaaa gtgatgcacc ggcagggatt
    14581 tatctctgat ggcggggaaa cggcccttaa ccgctgggtg aaatcgcaga cggccgcgca
    14641 gaacgacggc gaaggtgtgg ctaactggca gtggctggag caacaacccg cgctggcctc
    14701 tgatgtgctg gagcgtctca aacgctggca ccgccgcaaa atgctggcag ccctggatat
    14761 gtcggaacgt gcagtgatga gttatgagcg tgtttgcagg ctgtatgaaa aatcattccc
    14821 ccgttaaccc caaatcccgc cacagcgcgg gatttttatt ttaaacttgc cctgtgtaat
    14881 gatgaccgga ggcgggtatg gctgaaacac agatgagcat gtttggtgac agcgaacagc
    14941 tgcacgcgct gattgaccgt ctggatgata ttcctgatga tgagctgaaa aagaactggc
    15001 ccggcacgct gcgggacctg gttgatgtga tcagtgctga actgcaccgt caggggattg
    15061 aacctgctca ggcgggaatg cttgcccgta aggttgcagc cgcacaggcc gggtacatgg
    15121 gcgggcgtgg ttattatctc ccggtcggtg aatccctttt caccgaactg cgcaataatg
    15181 aaattttttc ccgctggagc cagggcgaaa aaattgaaac cctgcgccgc cactaccaga
    15241 tgtcagagac gcagatttac tccgttatcc gcaaccagcg tcgcctgcat caggagcgca
    15301 cgcagccccg gcttttctga tattccgcaa atcctgcccc cttttcctca cggttacgct
    15361 gactcagaga acaccatgag acagcgtaac aatgccaaaa ctccccgcac ccctgcgtaa
    15421 aaagctgatt gcccttgttc tggccggagc cgggacgttc accattgcca cgcactacac
    15481 cggctactgg gaagggaaag aaaacaccac gtacattgat cccaccggca cccccaccat
    15541 ctgttacggc cataccggcc cggatgtgaa accggggatg acaaaaactg atgaagagtg
    15601 tctggagctg ctggaaaagg acatgaaatg ggcctttgcg gctatcgatc gtcatgttca
    15661 ggtgccgctt acccgtggtc agacggtggc gctggcctcc tggattttct gggccggtga
    15721 aacgaacttt cgcaactcca cgctcctgcg ccttatcaat gccgggcaga tgcccgcgtc
    15781 ctgtaagcag tatatccgct ggatttattc aaagggggta aaactccccg gtcttgaggc
    15841 ccgccgttcg gcggatgaat ggttatgtcg ttacgatttg ccgaaagtct gaaccgcttc
    15901 tggcgaccgc tcatgattgc gctgctgtgt gccgttgtgc tgctgcgggg tgtgctgtgt
    15961 ctgcggtgaa caatccctta ccggccctga ttgccggagg ctgtatggcg gcgctggggg
    16021 tgatgagcgt gctggctgcc gtggtatggg gaatgcacca gaagacgcag cgccttgagg
    16081 acaataatca tgtgcttgtg cgtgaacggg acgaagcccg tctggtgctg gcaaaccagc
    16141 aacgcaccct gcaactcatt tcacaaatca gcaaggcggc cacgaatgaa aaacagcaga
    16201 acatgcagca cagcgaggaa cagcagagca ttgtccgccg gtcactggca acggtgcctg
    16261 ctgccgctgt gcctgtgcct gatgatgtgg ctgaccgggt gcggcgggcc gtctgtgaaa
    16321 tacgtgccgg tgaagccggt gccgatcccc gctgaatggc tggctgactg cctggtccct
    16381 cccgcgccgc agccgttcac gtttggtgca tcggtcactt acaacctgca actgctggcg
    16441 gtgattaaaa actgcaacgt ggacaaggcc agcatccgcc gtctggagac acagcgacag
    16501 catgaattta ctgatatggc cggaacgact gctgttccgg caggaaagac gaagtaagga
    16561 aaaaggtatg gatgattcag attgtgctca ggcagtcatg gagcgtgcaa cagaacgcgc
    16621 cctttgtgac cggctgacac gtaagcgccg ggtggcggag acaccgggca ggcgtgtatg
    16681 cgcggactgc gggggtgaca ttccggctgc ccgtctggtt gccgtgccgg atgccatccg
    16741 ttgtgtgaac tgccagaaca tcatggaggc cggtcatgtg ggtcagcatc gttaaagatt
    16801 atgtcgtgcc gatcctgtcg gcaacggcca cagccggggg gatcttcatg gcgctgatgc
    16861 gcaaaacctt tgtcccccgc gaggcctttg aaaaactctc cgaccgggtt gagcgggtgg
    16921 agagccggat ttccggcctg cccacgcagc aggaggtgaa cagactcaat atcgaaatca
    16981 ccaccctgcg cggtgagctt aaggccacca atgccaccct gaaatccgtc tcccatcaga
    17041 acgaactgct gctggagcag gctgtaagga aaaaacacaa tgagtgattt cattactgaa
    17101 gatcagcgtc ttgtcattct gcggtcgctg gcggattaca acggcgaact gggcgaatcc
    17161 gtgcttcagg actgcctgga tgattacggt caccgcgtgt cccgcgacac cgttcatacc
    17221 cacattgcct ggcttgccga gcagggactg gtgcgtaagc gcacgcttgt taacggttat
    17281 ttcatcgctg aactgaccgg ccggggtcag gatgtggcgg aaggccgcgc caccgttccg
    17341 ggtgtgaaga aaccacgcgc aagggggtaa cgatggataa gccagcgcgt ggccgtgtac
    17401 gcaaggtgga tttgctcccc gacagcatcc gtaagccgct gctggaaatg ctgcgtgaaa
    17461 aacgtctgac gcaggtccag atccgcgagg aaatcaaccg tctgatccgt gaagcaggtc
    17521 tgccggaaga gcagcagctc tcaccggcgg ccatcagccg ggaagcctcc cgtaatgagc
    17581 tgattgcccg taatctgcgt gatttacgcg agcagacaaa agccatgatg gccgaactgg
    17641 gggataagcc gaccggtgaa accacggcac tgattctgga gatgtcccgt gccctgatgt
    17701 accgccgtct gcgtgccgcc accgaatccc tgaacagcga cagcgatgtg gatatgcgcc
    17761 ttattaagga catcctcctg tcggcacagc gtgcagaaag tgccgccgag cgcagcatta
    17821 agcgtgaaaa agaaatccgg gccgcatttg ctgaagagat ggcgaatgcg gtcaccgatg
    17881 agttgcgcgg cgtggatggc atgagcgagc agatcgaatt ccgtatcagg cagatgctgc
    17941 tgggtaaggc gtaggaggtg aatcatggca acagataacc gctcactgat gagcagtgcc
    18001 catcagaaag cattcagtct tgcaacacag ttacctcctg aaaccataaa ggtatgtcgt
    18061 gagttgactg aactcagcat tgccggggaa atatccctgg aacaactgca ttcgctgctg
    18121 gcagaggctg ccactgatga gttgagcggc gtggacgtga tgactgaaga gcttgagtcc
    18181 cgaatcaaaa gcattctgac aggtaaagca tgatgaacaa agccagagaa acgtcgcctt
    18241 cactgatgca aatgacgcct ccccgcaaaa ttgatctgtc aggtgaaaaa gccctgctgg
    18301 gcgtggatgt gccggacagc cttgatttgc ccggtgatat gccggtgttt ctggattatc
    18361 aggcccgctg gtttgcagat gaaagtcagg tctgcatcgc ggagaaatcc cgccgcaccg
    18421 gtcttacctg ggcggaagcc gggcgtaacg tcattactgc tgcaaaaccg aagcgtcgtg
    18481 gtggccgcaa tgtgttttat gtgggatcaa agcaggagat ggcgctggaa tatatttctg
    18541 cctgcgcgct gttctcccgt gcctttaacc agctggcaga tgctgatgtg tatgagcaga
    18601 cattctggga tcgggataaa aaagaagaaa ttttgaccta catgatccgc tttccgaaca
    18661 gtggattcaa aattcaggca ttgtcttccc gtccgtcaaa cctgcgcggc cttcaggggg
    18721 atgtggtgat tgatgaagcc gcgttccacg agtcactgga tgagcttctc aaggcggcaa
    18781 tggcgctcac tatgtggggc gcgcgcgtgc gtattatctc cacgcataac ggcgtcgata
    18841 atctgtttaa tcagtacatt caggaagccc gcgaggggcg caaggattac agcgtccacc
    18901 gcataaccct ggatgatgcc attgcggacg ggctgtaccg tcgtatctgt tacgtcaccg
    18961 atcaggaatg gtcaccggaa agcgagcaga aatggcgtga tgatctctac aaaaacgccc
    19021 cgacccgtga ggatgccgac gaggaatacg gctgtatccc gaaaaaatcc ggcggtgcct
    19081 atatccccca cgcgcttatt gaaatggcga tgatccgcga catcccgatt ctgacgtttg
    19141 aagccccgga caacttcatc agccgtgccg catggctgcg tgaatcggaa gttttaacct
    19201 ggtgtgaaga acatttaaaa ccgcttttag agaaattaaa tccccgttcc cgcttcagct
    19261 tcggggaaga ctttgcccgc accggtgacc tgtcctgctt tgtgctgctg gaaatcaccg
    19321 aatccctggc aaagcgcgag gtgtttcgcg tggagctgcg caacctgccg tatgcccagc
    19381 aggaacaggt gatgatgtac atcctgaccc gcgttccggc gctggtcggt gcggcgttcg
    19441 acgccaccgg taacggcggc tatctggcag aggccgcact gctggccttt ggcccggaca
    19501 tcatcgactg cgtgatgttg tcgccgaagt ggtacggcga gtggatgcca aaactgaaag
    19561 ccgagtttga agatcagaat atcctcgtcg cccgccatca gaccacgctt gatgatttgc
    19621 gccatgtgaa ggtagtgaat ggtatcccgc aaatcgacaa ggggcgcacg aaggaccaga
    19681 acgcaacggc tgcgaacgcc cgccgccatg gtgattttgc cgtggcgctc tgtatggcaa
    19741 accgggcgtc atacatggag ggcttcatcc tggatgaatc agcctgtcag gcgctacagg
    19801 agcggtcgcg ggcaatggag ggcggttatc gtgatgacga tgaggcatat catgaatttg
    19861 atcggggggg ctggtagtgg gacgcatcat tgatactgac ggaaaacctt tctcctttga
    19921 cccggagatg cagagtgccg cgctggatat tccgcagatt gccagccgtt atattgaaca
    19981 tccggcttcg ggtatcaccc cgaaccgggc ggcgcagtgc cttcgcgggg ctgaacgtgg
    20041 cgatctgatt gcccagtccg atctggccgc tgatattgaa gaaaaggaca cccacctttt
    20101 tgcggagctg ggcaagcgac gtcttgccat tcagggggtg ccatggagta ttgagccgcc
    20161 accgaacgcc agcgcgaatg agaaaaagga cgcggaaatg ctcgacgaat atctgcattc
    20221 cgcagactgg tttgatgcca tgctgtttga cgccacggat gccatcctga agggctattc
    20281 ctgcatggaa attgagcacg ggatgctcgg taaaatgcac atcatccgcg ccatccgctg
    20341 gcgtgacagc gggcatttct gccttaaccc ggatgatttg agcgaactgc ggctgcgtga
    20401 cggcagccat tccggggtgg cgtttcagcc ctttggctgg atagtgcatc agtcgcgttc
    20461 acgcaccggt tacggtggtg caaccgggct tgtccggacg cttatctggc cgttcatttt
    20521 caaaaactat tccgtgcgcg atctggcgga atttctggag gtgtacggcc tgccgatgaa
    20581 ggtcgggaaa tacccgtccg gggcaacacc ggagcagaaa agtgccctga tgcgggcggt
    20641 gatggatatc gggcgacgta caggcgggat catcccggcc gggatgtcgc tggagtttca
    20701 ggcggcagcg aacggtcagg ccgatccgtt tgaaaccatg atttcgtggg gggagcgttc
    20761 catctccaaa gcaatactcg gcggcacgct gaccacggaa gccggagaca aaggcgcgcg
    20821 ctcactgggt gaggtacata acgaggtgcg ccgggaaatc agggattctg atttacgaca
    20881 gctggctgca accctgaacc gcgatctggt gtatccgctg tacgccctga acaccacgca
    20941 cactattgat atccgccgtc tgccacgtat ctgcttccag acaaaagaac caggtgatat
    21001 caccaaaatc accagtgcgg tgatgcagct cagtacgggg atggatattt ctgatccctg
    21061 gatacgtgag cagaccggta ttccgcagcc cacgcccggt gaagccatat tccgtgtccg
    21121 tcagagtggc aatgaaccgg ctcagaccga cagggaaaca ccaccggaaa aacaggaaaa
    21181 aacagagcag acggcgctgt cagccagact gccggaagcg aaaagcacgc cgcgtgatga
    21241 actggatgat atgggggacg cggtgcctgc ccgccgctta caggaggcca tcgacccggt
    21301 actggagccg gtcattgatg ccatcagaac gcgggggctg gcggatgcgc tggcagacct
    21361 gcctgccctt tatcgtgaaa tggatgattc ccgcctgatg acgctgctca gtgatgccat
    21421 gtttgctgcg gaaatgaagg ggatgctgga tgacacaggg gattgattta ggttatgccg
    21481 ccacgcttcc ctcaaaagag gcggtagcat acttccgtgt caaaggggcg catatcagct
    21541 ggaactggtt cgaaacagcc gcggatgttc atgcccgctc attcacggca gcaaaagcgg
    21601 cacgcctgga tgtggtgacc acgttacagg ccgaagtgca gcgggccatt gatgagggaa
    21661 tttcacagaa agcatttatc cgcacgctga cgccacgcct gcaaaagctg ggatggtggg
    21721 gaaagcagat tgtggtggac agcgacggta acgcggaaga agtgcaactg ggcagtcccc
    21781 gtcgtctggc gctgatttac aacgtgaaca cccgggtggc ttacaatgcc gggcgttaca
    21841 cgcagatgat gaacaacacg gacacgcatc cgttctggca gtatgtggcg gtcatggaca
    21901 gccgtacccg cccgtcgcat tccgccctta acggtctggt attccgctat gacgatccgt
    21961 tctggaaaac acactaccca cctaatggct ggaactgccg ctgccgtgtg cggccattgt
    22021 ctcaggctcg tctggatgca atggggttat ccgtttcatc cggtgaggat catctctcca
    22081 cccgcaatgt tgaggctggc gtggataaac agaccggaga agtcagagaa atgccggtga
    22141 ccacatattc agatggcacc agaaccatga caccggatgt gggctggtca tataaccccg
    22201 gctcggcagc gttcggcaca gaccaggcgc tgatccgcaa actgatcgag gtgaaaagcc
    22261 cggcgttacg ggaaatggtg gttcaggaga tgaacaacag cccggagcgg cagctggcgt
    22321 tccgcatctg ggcaaaaaac atcatgcaga cccggcgagg tggtaacgat atccgcacgc
    22381 tgggctttat gactgaaagc attgcgcagg cagtggaaag ccggacggga acgccaccgg
    22441 cccgcctgct ggcgatgagc ggtaaaaatg tgctccatgc agacagtgtg aaacatcaga
    22501 atgacggcat cgccctgatg ccagaggact tcgggcgctt accggcaatg ctggcaaagc
    22561 caaaagcggt gctgtgggac aaacggcaca aaaatctgat gtatatcgtg gaaaataagg
    22621 atggcagtgt gcagattgcc gttaatgcac cgtacagtct gaaacgtcag cccgacaaac
    22681 tggatgtgat tgtgaatgct taccgggtga tcaatatgga taagctgaaa tcggatatcc
    22741 ggggcggaat gcttgaggtg ctggaaggac aaattgatta acggtcagtg gcgggggtcg
    22801 aacccgcata catgatccac ccggaggcag gcaccgactt taccagttaa gcgtacactg
    22861 accgcctgtt tattttatat tcatccttta tgcggaggca atatgtcatc cattgatgcc
    22921 gctgtcgttg ttgacgttac gcgtctccag cgggtgtttg cccggcttca gtttgtgggc
    22981 ggcggaaaag acctggcccg cagtgtggcg gcgagtcttc tgtcatcgtc agaaatggcg
    23041 tttgaacagg aaaaagagcc ggacggcgaa cgctggcatg actggtcaga tccttaccgc
    23101 aagtggcgta cccgtaaggg atacacgccc ggcaaaatcc tgacactgaa cggcgatctg
    23161 gcccgccgcc tgaccacgga ttatggcgat acctgggcgc tgattggatc aaatgaacct
    23221 tatgcggcca ttcatcagtg gggtggcctg cctggtatgc caccgggacc ggcggccatt
    23281 ggcgcacgtc cgtatatggg ctttgatcag gtggcagaac aggagatcat ggacgaaatc
    23341 agaaaacgct ttaaaaaggc cacagaaacg ccgtaatggt ttaaggtata caaacgcatt
    23401 accttaccct cttcaggcgc gtgtcgtgat tttctaacct gtatttaacg ggctttaaaa
    23461 tctgcgtgaa gcgcctttct catttttccc ctgtcagcac tttctgaagt cccgcaaaac
    23521 cggccccgaa aaacaccggc ttatgctgcc ggaatgaaga cgaaaaacac gcccaaactt
    23581 gcttatgcca ttctgaatgc catcagcctg tccgcagacg gggacggtga ctggtgtcag
    23641 atcatgccag ccggtcgggt aaaggcccgt gacggtcgcc cggaaaaacc ggcggaaggc
    23701 tggctgatta accatgcggc cgttgagcgc atggtatccc gggttgtggc gctcaatcag
    23761 ccggtaaaaa ttgactacaa ccaccagacc ctgattaagg ggcatccggc accggctgcc
    23821 ggttttgtca tggcctcacc ggaaaacttc cgcttcagtg aagagcgggg ttttgaagtg
    23881 cgtccggact ggaaccctcc ggccattgaa cacctgcgca ataacgaatt tccctggttt
    23941 tccccggtga ttggttatga cgaaatcacc ggtgaacctg ttgagcttcg gatgctggct
    24001 attaccggtg accccggtct gactggcatg aatcctgtcg ccgcactgtc ggcggatgac
    24061 ctttataacg ccttaaaccc tcctttaaag gacacctcca tgaatgagca attacgccag
    24121 ttgctgacgg cgctcggtct gaccgtggcc gacggtgacg aatttacacc ggaactgggc
    24181 acggcggcgc tgtctgccct taccgggatc aaaacccgtg cggatgcgca cgacaccctg
    24241 aaaacgcagg ttgccagcct gtcggcagag ctggaaaccg caaaaggcac accaactggc
    24301 ggcactattg atctgacgaa atacgtgccc gttgagacgt ataacgccct gcgtaccgaa
    24361 tatgtcgcgt tgtcggcaca acacggcagc accacgctgg agcagttgct ggataaggcc
    24421 gagtccgaag gacgcatttt caaaagcgaa cgcggataca tggaaggact cggacaacag
    24481 attggtgttg cggcactttc tgcacagctt gacgcccgcc agccggttgc ggctctgacc
    24541 actcttcaga ccgacaccgt aaccgtgccg gataaaaaga ccgccaccgc tgcgctgtcg
    24601 gctgaagata tcgccgccgc ccatctgctg ggtaaaaccg aagccgagtt cctgaaaatg
    24661 aaagaggaaa tgcaataatg cctaccccga tcacaccggc gatgatcacc gccctgatga
    24721 cgggctaccg ttctgatttt caggccggga tgtccatggc tccgtctcag taccagaaaa
    24781 ttgcgatgac cgtaccgtcc acgtcgaaat ccaacaccta cggctggctg gggcagttcc
    24841 cgcagttccg tgagtggatc ggctcccgcg tcatcgagaa gatgaaagcc tatggctacg
    24901 ccatcgtgaa caaaaccttt gaaggcaccg ttgccattag ccgcgatgac tttgaagacg
    24961 acaatcttgg catctactcc ccgttgttcc aggagatggg gcgcgctgcg gcggcacaac
    25021 cggatgaact ggtctttgct gcactgcgtg atggtctgaa tgcggcctgc tatgacggcc
    25081 agaacttctt tgataccgaa caccctgttt accccaaagt ggatggctcc ggtgatgcac
    25141 agatggtcag caatatgttt gtggcaaaaa ccggctctgt cggtgcacag gcggattaca
    25201 gcggcccggc ctggtatctg cttgactgct cccgcgcggt aaaaccgctg atttatcagg
    25261 atcgccgcaa ggccgaactg gttgcccaga caaaagtcga tgaaggccgt gcgtttactg
    25321 ataacgagtt tgtgttcggt gcttccgccc gtcgcaatgt gggctacggc ttctggcaga
    25381 tggcctacat gatgcagtca ccgctgacgc tggatgcgct gtggcacggc tggtcagcca
    25441 tgcgtgactt taaggctgac ggtggccgca aactcggcat caaacccacc catattgttg
    25501 tccccacctc gctggaaaaa caggcggtgc agctgctgga gcgcgagctg ttcgcggacg
    25561 gtaacaccac cgtctctaac gagatgaagg gcaagctgga gctggttgtc gcggattatc
    25621 tgtaaacggt gagccgggct tacggcccgg cccctgcgga gggcaaatat gaatgaacat
    25681 cacactgtgg cagcaacaga tgattcaggc cttcaggtca gtggtgacag tcctgtcacg
    25741 gtactggcag aagtgcgttg cagtcgtgcg cagtttcgtc gtgcggggtt cctgttcatg
    25801 cggggacgcc agcaggttga cgtcaccccg gagcagcttg cccgactgga agcggagcca
    25861 tgcctcactg tacgaatact acagacgcct ggtgatgatg cgggagccgt ggcgggtgtg
    25921 gttcatgcag agaccggtgc agatttagcc gaagccggac aggatgctcc ccggaaaaag
    25981 accggcaata acgccagaca ggcgaaaaag gctcgcgcat gaattacgcc accgaaaccg
    26041 atatgcgggc acgttaccgc gaggatttgc tcaggccatt actggccgtg ccccgttcgg
    26101 atgagccgga cgcgcgcaag ctgaaccggg cactgacgga cgcgtctgcc cttatcgaca
    26161 gttacctgtc tgcccgttac acgctgccgc tggcggtggt tccggcggtc cttgttcagc
    26221 attgctgcac gattgccttt tattacctgt gcgatcagcg ggcatccgat caggcgcgtg
    26281 accgttaccg tgaggcgctg gcctggctga aagatgtcat gaacggtaac gtgccggtcg
    26341 gcgtggatac agacggtgcg gcccctgaat ccggggattt accgcaggtg cagtctgatg
    26401 cggcggtgtt cgggcgcaac cagaagggct ttatatgatt acggaaaccg agcaagcgta
    26461 catcgcccgt atccgtgagt atttcgggaa tgaactggtg tcagttgaca cgcatcccgg
    26521 cgactggagc gacagtgtgc tgcgcaccat gctgattagc gccccggcga tctacgttgc
    26581 ctggctgggt gccggtgaag gccgtacccg tgggcgtctg gtcagccact gggtgttcta
    26641 cgtcatcggc gacatgctca atggccgtga ggtcagccgt cccgggcttt atcagattgt
    26701 ggcccgcctg attaccgtgc tggacggctt cagaaccgaa aaaacctcac cgctttactt
    26761 tgaaaaggcg gtcaacggtt acaccgaaac gcaggccgac agtggcgcgg tgatgtatgc
    26821 gctttatttc tcctgcgagg aaatgatcgc accgctgact gatatcagct cgctggatga
    26881 tttcctgcgc cattacgaaa ccttcaccga accggaaggc acaccggaat ttaaggcgca
    26941 catcagcctg ccaggtgcaa ccgccgccgg tgaaaaagcc ggaccgtcag aggaataaca
    27001 gatgacaacc atttttatca aacccgcgcc gggatgcctg atccgcgatc cggacaccat
    27061 gaaaccgctg gcgcaggagg gagaggaaaa acccttcacc ccgttctggt gccgccgtct
    27121 taatgacggg gacgtcattg tggtggaaaa agcggctgaa gcagcaccgg cagcagcgtc
    27181 tgcaacgact gatgccgccg gaaaacccgc gggagcggca gcagaaaaac ctgcgactga
    27241 aaaacccgcg actgaaaaac ccgcagcatc agacaagggg acgtcctgat gattaacttt
    27301 gattatatcg gcgataacaa tcgcattccg ctggtacaga ttgaaatcaa taattcaatg
    27361 gccgtgaccg gcactccacc acagcgtcag gcggtactgt tgtttggtca ggctgcaatg
    27421 aaagacacca ccattcaggg gcgcggtcag cttgatgtac cagtacgtat tacccgagct
    27481 tcacaagcaa gagaactctg ggggcgaggt tccatgattg cactgatggt agaggaattt
    27541 atcgccatta accctgacac agagttatat gccattgcac agggggttgg aacaggtcag
    27601 tccagggcat gcgtgatgaa tatcattgga acagcattgg cagacggggt gttaagtgtt
    27661 tacatcggcg gtcgccgtta cctcctgccc gtcagtaagg ggaaaaaagg taaagagctt
    27721 gtggaagagc tggctaaggt cattaacgct gacactgatg cgccattcac tgctatcgca
    27781 gctgaagtca gtggtgataa tgctgaagga cttaaaggga gcatgggggt taacgcccgc
    27841 tttatcggtg aatgctcagc tcatgattta cgggtgaact attatgacgg tgaaactaca
    27901 ccggccggtc ttacgctgga gctcgtcgcg ccaacacaga aggcaacaaa ccccgatatc
    27961 acccgtagcg tctcagggat ggggaatcgt cagtacaact acatcgtcat gccctataag
    28021 gatgcggcaa atctcaaaat tctggcggat gaactgctga aacgctgggg accggtaaaa
    28081 atgtcggacg gtatggtctg gatggcgcat accggcacct tcggtacggc aaccagtttt
    28141 ggtgaatccc gtaatgactt cctgtttacc tgcacttcta ttcctaaagc accagagccg
    28201 gattatgtct gggcggcctc catttgtgcg gtctgtgcgc cctccctgtc aacggatccg
    28261 gcccgaccat tacagacact ggcgctgcct gcccgtatgg cacctgaagc accggctcgt
    28321 ctgacgcggg aagagcgaaa cagcctgctt tataccggca tgtccacggt gaccgttgcg
    28381 gccgctgatg tggtgcagat tgaacgtcag gtgacgatgt accgtcagaa tgcatacggc
    28441 gaaagcgatc ccagctatct ggatgtagaa accatctaca ccctgtcata tctgcgttat
    28501 tccctgcgca cctttatcac gcagcgtttc ccgcgccaca aactggccga taacggtact
    28561 cccgtccggc ccggtcagaa tatcgtgacg ccggagatta tgacgctcca gctgattgca
    28621 ctgggggaag aatgggtgga actcgggctg gtcgaaaatc tggacacctt taaaaagaat
    28681 ctgctcgtgg agcgcaacac aaaagaccgt aaccgtctgg atgtgatgtg tacaccggat
    28741 ctggttaacc agttccgctt catggccgcg cagatccgtt tcattttgtg aggtaaggca
    28801 tgagcggaaa acagcatcag ggcacggcca ccatccgtgt gaacggacag gagtacgcca
    28861 cccttgaggg agccacgttc tccccgtccg gctttgagcg tgaagtggtg aaaggcgcga
    28921 aagtctatgg ctatcgtgag aaaccccgtg aggcgacgct ggactgtaaa tttccggctg
    28981 gcggagaagg ttcaccggct gccgatgaaa tcaacacctg gactgcggtc acgattgagt
    29041 tcgtggccga taccggtgaa gtccatatga tgacgaaggc ctggagcagt gaaccggcct
    29101 cgcttgacgg tggcggtgat atctccgcga agttcgccag cgccaccagt acccgtgtcc
    29161 agtaatcagg aaaaatgcaa tgaccacacg taagaaaaaa acggcggttt ctgacgaggc
    29221 cgtgatggag gctatccggg atgtacttga agacggcgat ccccgcaccg ccgggctggc
    29281 tgaacagctg gcgaacggct atgtggatct gctcgacggt ctgccgttcg gggaatcccg
    29341 tgaataccgc gtcaccttcc gcgaactgac ggcgaaagac agcattgacg cggaaaccga
    29401 ggccgagcgt cttatggaaa cccgaaatgg cggcccggtt ctgatagcct cgccggcact
    29461 gcgcggcatt gcactgctgc gccgtcagat tgccgctgtc ggccagattg aggggccatt
    29521 gtctccgcgt cagatcggac agctcagcga gcgcgatctc tcccgtctga tggcggcggt
    29581 aagcctgctt gattccgcga tggcaggaaa gctggtcgct gaacgggggc gaccagatgc
    29641 agtgccggga tcagattgaa gaagccgcaa ttgtgctggg gatggtgaca aaaagcggcc
    29701 cggagtgggc gcttaacctc cccttatcgc agctttaccg gcactgccga cagaccgaaa
    29761 aaattctcag aacgaagcag taacccatgg caaaaaacct gaaagcctcc ctgattgtcg
    29821 atttactcgg caatatttct gccaaatccc gccagtggtc acaggagctg ggggcattct
    29881 cccgttccgg caggacgggg ctgggtggtc ttggaaatgc agcacgtcgt gccggtcagg
    29941 agacagaact tgtcggctcc cgtatgcagc gaacgcttgc cggtgttcgt ggcagtatcc
    30001 gcacggtgac gtcagatttt gaccgccttc agggcagcat taccggcacc atcgggcgga
    30061 taagcaacct ttacgggatg ctggcgggag gggctgcggt ctacgggttt aataaagcct
    30121 ttatccgccc ggccgcagag atggagaact atattctgcg tctcaacgcc attaatcatg
    30181 gcgataaggc aaaaacggaa gcagtgaaag cctgggcggt acagaacgcc aaagacacca
    30241 cctgggggct ggcgggggtt atgcaggaat acgcctccag tcgcgggttt ggcatgagtg
    30301 acagggaagc ccgtcgcttt attaccatgc ttcaggatca gggcggctat cacggctggt
    30361 cactgtcaga ggcacagggt gcgtccctgc aactcaaaca gatgtttgcc cgccagagta
    30421 tccaggcagc ggacgccaat attctgaccg gatacgggat taacgtttac cagttactgg
    30481 ccgataagct gggtgtaaac cagaagatca tccgtgagaa aggcgagaaa ggaaaactgg
    30541 gaccggacag cattcgcctg ttatttcagg tgatggccga acaggcaaaa ggtgcccaga
    30601 aaaacgccat gaactcatgg acgggaatga cgtccatgat gggtgacgtc tgggatcagt
    30661 ttgcccgtga cgtgatggcg aagggaccgt ttgacagcct gaaaaagagc ctgaaaggct
    30721 ttctggatta tgccgatgcg gcacagaaaa gcggcttaca ggataagctg gccacacaga
    30781 cggcctcagc actgaatcag ggatttgagt atgccagaga tgctgcgacc ggcttttacc
    30841 gggccattca gaaagtcagg gaaacgcttc aggcactgcg cgacgccggt tatggcgatg
    30901 cgctggatcg tattggtcag ggtgcgcaga ccgccgcaaa gtacctgatg tatatgtatc
    30961 tcgcctcccg tgcgctgaag gtgctcaggt ttgcagggac gggggcatta cgtctcggcg
    31021 caaccccctt acgctatggc atggcgatga cctccgtgct gacgtcgccc ttccgcaaac
    31081 cgcagaccac tgtaccggga acgcagcccg gacgcgctgg ccggttcctg aatttcctta
    31141 ccggggtaaa tccggctgcc gttcagcccg tgctggtcac caactggcct gcgggtggcc
    31201 tggcctcagg tggcggggat gtggtggtca gcggtgacgg caaaaccgtg cgcggccgta
    31261 aaaaacgcgg ccccggacgt ggacggggtg tcacgaccgt cgtgactgcc ggtgagcaac
    31321 tggcggagag tgccggtaaa cagggcttct tcggtcgtat gatgagccgt gccggtggtc
    31381 tgctaacggc ggccggaaac cgcatggggc tggggcgttt tgccgggctg ttccgtggtg
    31441 ccggtcggct gggtggtggt gccctgtggg ccggtgccat ggcggctcct gtactgctgg
    31501 acagcagcgc cagtgcagcc gataaagccg gtgccgtggg ttctcttgcc ggaagtatcg
    31561 ccggtggcgc gctgggggcg gctgccggtc cggtcggggt tgccattggt tccacggtgg
    31621 gcagctatct cggtgattat ctggggggct ggctgacgca ggcctggcag aaactgcgtg
    31681 gcagcagtga cgaaaacggc ggacaggcca ccgcgaaaac cgccgcacgg gtggagcttg
    31741 tggcccctga aggctggcgg gcgcgcagta ttgatgtgga tgataccgca cagcatggcc
    31801 tggatgtgaa cgtctggaac ggagggaact atggcctcta ctgacacgcc ggggcgtggc
    31861 tcctttcgtg gcgtaccgtt cctggtttat caggagcagc gggaacgggg cggacgtaac
    31921 attgtgcgcc gtgaataccc actgcgtgaa agcggcggtg cggatgatct ggggccaaaa
    31981 ctcccggagt tcacattcac ggttctggtg accggagacg atcttcagac acagcgaagc
    32041 cgcctgcgtg atgcgctgcg tgcacccggt gccggtgaac tgatgcaccc ggattacggc
    32101 acgttaaacg tgctgataaa cagctttgaa agccgttata acgccagcga gcagggaacg
    32161 gttgagttca cgatcaacgt gatccccgcc agtgacgata ccgcccctgc ggtggcagaa
    32221 gatacggcag ctgcgctgga gcagaaaagc ggctcagcaa tgaatcagct gtttaacacg
    32281 ctttcagacg gctggacggt gatttcagac ggtctgcatg atgtacaggc catgaccgac
    32341 accatcagcg ataagattga tgcactggag aatgccgttt cggggatggg gattgtgcag
    32401 gatatcagtg cctttacggc cagttttact gccctgaaag gcaatgccgc cgccctgatt
    32461 aatgcgcccc gtcgcatggc tgaatcgctg gccggaatgt ttgccgtgct gaccgggctg
    32521 cccggcgatc cttccctgtc tctgaccgga aaagccggaa cgccgtccgg cagcctgacc
    32581 acaaaccgtg acagcatggc agagcaggcc atgccgcagc tgtaccgcac actttcctcc
    32641 ctgcgttaca ccctcagcga acaggatgat ccacagcgcc tgattggcct gacgcctgcg
    32701 gcacagaaaa atatccgtct gctgcggagc gtgatgcaga gtgcaaccct ggtgtcgcag
    32761 gcacagacgc tcggaaaact gctggatcag gtcatccggc agaatacgac gcacgttcag
    32821 gatactgacc gttcgggcgg actggcctgg ctggaaagtt ctgctgatgt gcagcgtatc
    32881 aaccgggatt tgagcgatgc catggaacaa caggtgctgg atctgtccgc acagggctat
    32941 accgggactg cccttgcatt acgtgatgca gggcttgcgc tgacagaaga tttaaccaca
    33001 cggagcgtgc atcttcccgg tgcttttcag gtcatggttc gcaccaccga accggcactg
    33061 gtcacgctgt accgtgccac cggcaacagc agtcgctggc agtattttgt ccgcagaaac
    33121 aatatccccg atccggtttt tgttccgggt ggccgcagcg tggaggtgat cagtgagcag
    33181 caggattgaa ctgtatatcg gtggcagtat tttttccggc tggctgacgg tcagcgtccg
    33241 tcgctcgctg gagcatctgg ccgggtcgtt tgaactgggg ctgatgctgc ccggtgagcg
    33301 catcccctca gccctgcgaa ccggtcagtc cctgacgctc agaattaacg ggcagacggt
    33361 catcagtggc tggctggatc aggtcagtca gcggatcagt gcaacccgtc atcagatcag
    33421 catcagcggt cgggataaaa ccggcgacct ggtggactgt gccgccatcc atcccggcag
    33481 ccagtggcgc aaccggacgc tggcacagat tgccgcagat ttatgtgcgc ccttcgggat
    33541 agccgtgcgc tggcaggtta acgacgacac ggctgcacgc cccttcagct cttttacgct
    33601 ggaaaactca gaaaccgtgg cggatgcgct gacccgtgct gcccggcatc gcggggtgct
    33661 ggtgaccagt aatgctgacg gcgatctggt gttcacccag gcaggaagcc agcagacgga
    33721 cagactggtg ctgggagata acctgcttga tgccgattac aacacggact ggcgaggacg
    33781 atacagcgaa taccgtgtcc ggggacacgg gcgcggtggt ggcaaacggg gagacagcga
    33841 atccgccgcc cgtctggcag cacctgtggg cgtcatcagt gatgagcaga tcggccgcta
    33901 ccggtcgaaa atcatcctcg ccgatcaaca gacagacacc accggtgcac ggcagcgtgc
    33961 cctgcgtgaa atgcgccgtg cgattgcccg ttcagaacgg ttttctgcca ccgtgcgtgg
    34021 ctggttccgg gatgatggcc ggttatggga tgtcaatctg ctgaccggtg tttcagccct
    34081 gcgtttcggt atagaacaga ctgaactgct ggtctgtcag gtggagtttt tacttgatga
    34141 acataacggg gaagtcaccc ggctggtact ggcaccgcgt gacggcttta tcgttccggc
    34201 agagccggac agtaagggcc ggggtggttc cggtgacgat gttgatgcct ttattcgcca
    34261 gcagatgaaa aaacagggga tcagctttga tgaatgatga agtgttcagc cgcctgattg
    34321 ccccggtaac gcgcggtatt cgcctgttgt ttggccgggg cgttctgacc ggcacacatg
    34381 acgaactgaa aatgcagaat gtgcagctca ccggcatgga cggcgaaacc tttgatgatg
    34441 tggagcgtcc ccagcagtac gggcagatca gcgttcccct gccgggtgcg gaaacctttt
    34501 ttgcctgtct gggcggacag cgggatcaga ctgtggtgct tgtggtggaa gaccggcgca
    34561 gccgcccgac cggactcaca gccggagata cgggggtgta tcaccatgag gggcaccgga
    34621 tacggttaac aaaggatggt cgcattattg tgacatgcaa aatgctggag atttacgccg
    34681 atgaggggat gcgggtggat acgccggagg ccacctttac gggcaatgtg acggtggata
    34741 aaaacctgca tgttaagggt aatttcgtgc ttgatggtac aggtaaatca gacgggctgt
    34801 tcacgatgtc tgatgccgtt attgccggga tcacctattc cggccatgtg catcaggata
    34861 acggcaaagg cagtaagaca ggagcgccgg agaatggctg atattgcaat tgtatgggat
    34921 caggggtgcg gttcgctgca actgaacggt gccgacttgc tgacggatga cagtctgctg
    34981 acggccgttc tgatttcgct gtttactgac cgccgggcgc tggcatcgga tgaaatccct
    35041 gacggtacgc gcgaccgccg gggatggtgg ggagacagtt ttcgcccgcg ccccattggc
    35101 tcccgtctgt ggcttctcag ccgggaaaaa acgctggcct cggtgataag ccgtgcccgt
    35161 gcttacgcgg atgaggcgct gggctggctc agtcaggatg gcgtggcgtc atccgtggtc
    35221 tgtcatgcag aacgtgtggg acacgcgcgg ctggcgcttt cggtgcgcat aaccctgcct
    35281 gatggttcag tgaggcccat gattttttat gctgatctta agggggagta atgccttatc
    35341 agcctttacc gctggcacag ttaatcacgc agacacagca ggatatcagc cagcgcctgc
    35401 ccggctcgca gccgggtgtg aatgaaacca ccctgaatgc cattgcttac gctcaggcag
    35461 ggttatctgc tcaggagcat gagcatctgg cctggattgc gcgtcagatc atcccgaccg
    35521 aagccgatga agccgaactg ctgaaacact gtgcattctg gggcgtcatg cgtaagcctg
    35581 cctctcgggg ggacgggccg gttcagctga tgctgaccac ggatgcgggg atcaaggaag
    35641 gtgtgcttct ccagcgaagt gatggcgtgg tttaccgtat caccacctcc ctgaccggta
    35701 aggccggtac gctgaatgtc agtgtggaag ccgaaagtgc cggtcgtgcg gggaatgccc
    35761 cggcagggac aaaactgaca tttatcacac cgcaggcggg gatcaaccag acggccacgg
    35821 tgaccggaac ggggatcacc ggtggtgcgg atgtggaaac cgtgccggag ctgctttccc
    35881 gtctggtttt ccgggtgcag aatccaccgt ccggcggcac gcagtatgat tttgaacgct
    35941 gggcgcgtga agtgccgggc gtgacgcggg catggtgccg cccggaatgg ccgcaggcgg
    36001 gcagcgtggg ggtaacgttt gttcaggata ataacccgga tattttcccc ggtgacggtg
    36061 atgttcagcg ggtggcggat tatatccgca gtcatgatga tccggcgacc ggccagcctg
    36121 tcggacagcc tctggggccg acggtgaccg tgtttaaact gaccaataag ccggtgccct
    36181 tcagcatcag gatcatcccg aaaacaccgg agaatcaggc ggccgtaaaa caggcgctga
    36241 ccgacctgtt gtataacgaa tcccggcccg gcggtctggt tctgccgtcg tctttctggc
    36301 gggcggtggc aggggtgaaa aatctggagg attttgaagt gcgcagtccg ctgacgtcag
    36361 ttcaggcggg agacagtgag ctgctgacgg taggagaaat cacatggctg taaccctgac
    36421 cccgcatcag cgcgccctgt tgcagttact gcctgacggg ctggcatggg acaagcgtcc
    36481 gtcatccgta ctggcatcat tatgtctggg cctcagtcat tcgacagcgc gtgtatcctg
    36541 gacgggtaaa cagctgcttg ccgagcgttt ccctgacacg tcgcgcctgc tgctggaaga
    36601 ctgggagcgt tatctcgggc ttcccgagtg tgatatggcc ggagccaccc tcacggagcg
    36661 acagcgttat gccggaaata aataccggat gaagccctcg ctgaaccgtg aattttatat
    36721 ccggtttgcg gcggagtttg gttatcagat tgacattcag ccctcaccgg attcgcagtg
    36781 ggtcagtatt gtcacgatta acagtgaaac cggctaccgg aatatgaatg tgctggatga
    36841 tattctcacg ccgctgcgta tttatgaagg cggtgcgctg gaatgtattc tgaaccgtta
    36901 taagcctgca tggcagacgt ttatttacgt ttatgcaaac agccatgaag aggagactat
    36961 ttaatgtttc atgttgataa taattccggc gtggcgaata tgcctgcgct ggcaccggcg
    37021 cagagtaata ccaccacctg gtttaccgaa ggtgacggac aaaaaggtat cagctggatt
    37081 ggtcaggact ggctgaatat tctccaggcc gaactgctga atattctggc tgaagccagt
    37141 attcagccgg ataaggcgca gttaaaccag cttacgctgt ccattaaagc cattatcgct
    37201 gcgaatgcct tttcccggaa aaataacctg aaagaaattg ctgatgccgg tgcggaggcc
    37261 cagcgtctgg cccgtggtta tcttggtctg gggacgcttg ccacaaaaaa cagtcttggt
    37321 cccgctgacg ttaatgccct ggcgaaggat cagaatctcg ccgacctgga gaataaggga
    37381 accgcccgta ataatctgga tgtttacagc aaaagcgaag gtgataaccg ttacctgcgc
    37441 agggagcaga acggcgcaga cattccggat aaaggggctt ttatcgataa cgtcggttta
    37501 cgggaaacgg taaacagggc ggcgaatgct ctgccatcgg acggcaccgc cgttgccgcg
    37561 aatagactcg ccaccccaag aaatattaat ggggttccct ttgacgggac gcaggacata
    37621 aacatcactt ctggtataac acagcaggat gcggattccc gatatatcca ggatatcagg
    37681 ctgggagcag agaacaggca ggtaatgtac ggtggggcat attactctgc gtacggtaat
    37741 gttatggcag gcctgcagat tgacagtaaa gttgatgctt caaacgattt tatagcattc
    37801 agacccctgc aaaaattagt cggaggtaca tggattacgg tatctcagtt ataaatagtc
    37861 agtaaataat atattttaaa acagatttat ttccaggagg attttatgga actaaaaaat
    37921 ataacccgtt attatcctga aaatatgcca tatggtaata atgttcagta tttccagagt
    37981 gaagatggta aggatttcta cgaatccctg ccattattca ccaaaaaata taaactttgc
    38041 ataacacctg acagcggcgt tatctgctca atatcacagg acgcctcggc gctgtatcct
    38101 gcggggtttt ctgtggttga ggtcgatgaa ctccctgaag gcacggatat ttcaggaaac
    38161 tggaagtttg ataatggcat aatctcccgt attccggtta actacgcaag aaaactggaa
    38221 gcaatgcgcc agtcatatct taatcaggca tatgaaaaaa ttaatgactg gagaacggag
    38281 ctacaactgg gcaccatcag cgatgaagac agagcagcac ttacccaatg gatggcatac
    38341 atcagtcagg tgaagaaaat ggaactaccc gccattaaaa cagaagctga atttaacgcc
    38401 attaaatggc ctgagcagcc acagtaataa agaaggccgg aaggccttct gaatatcatt
    38461 atatacttgc agccgtcacc catgctccat ttaacagata ctgcaacggc cggtaataca
    38521 cgccgccgac gttatctgcc gtattcttac cgacatcctg tactttaatt ccggtaagaa
    38581 cattgccgcc ccccagattc agatcccagc tttcccatgt tccgggcata tgtgcggtca
    38641 ccgaccccag tcttatggcg gttactgttc ctgatgagat agtgatatcc tgcgtcccgt
    38701 caaagggaac accattaatt gtgtgggcat ttgccagcct gttcgcggca acggcggtgc
    38761 cgtccgatgg cagggcattc gccgccctgt ttaccgtttc ccgtaaaccg aggttttcag
    38821 gaaagccagg attaactctg ttttattcct catgcgacaa ttccggcttt ttataataag
    38881 gaaatcgtat gctgattggg tatatcaggg tgtcaacaaa tgaccagaat acggcgctac
    38941 aaagaaatgc gctggaatgt gcaggatgtg agctgttttt cgaagacaga ataagcggta
    39001 agacaaggga gcgtcccgga cttaaacgcg cattaaagcg actccggcca ggggataccc
    39061 tgctggtctg gaaactggat cgccttggac gcagtatgca gcaccttgtc cttttaacag
    39121 aagaactccg gcaacgcggc gttaacttcc gcagcattac ggacaatatt gatacatcca
    39181 ctcccatggg acgtttcttt ttccatataa tgggcgcgct ggcggaaatg gagcgggaac
    39241 tcattattga acgtacacgc gcgggtcttg ctgctgcccg tgctcagggg tgtcatggcg
    39301 gcagacctca gaaactgaca gagaaagagc gggagcaaat cgcccgtttg ctggctaatg
    39361 gcgagtcccg taaacaaatc gcactgatat ttgatattgg cctttccacc ctttatcgtt
    39421 attttcctgc ctcaccagct ccgtcaccaa aagtaccgtg acgctttttt gaatttccgc
    39481 aaaacacata tttttcagcg gtcgctattc tgactctgtt taattcagaa acaggcagaa
    39541 aagacgatgt cagactccaa gtggaaaatg ctctccgcca tgcccggaga gttttcagtc
    39601 aaggttgccg gtggcacggt ggcatttatt gaaagccctt tccgcccttc gggtaataaa
    39661 ggggggatca ccttcgcaga ctgcgtgatc cgcttcagca cgaaagagcc attatgggta
    39721 atgccggtat ccggtaatcc cagtgcagaa atcaccagtt caggcgtgac tggcatcatt
    39781 ccaatcacat ctgacgtggc cggaacgctc acgccctccg actggaatgc accggataac
    39841 gccgggcctt ccggtagcac gaatggcagc agttcagagc cggaatatta ctttgttgtc
    39901 attctcgcgg gtcagtcaaa tggcatggcc tacggcgagg ggctgccgtt accggattcg
    39961 tttgaccgcc cggacccacg cattaagcaa ctggcacgcc gcagtacggt aacgcccaac
    40021 ggtacgtcgt gtacatacaa cgacatcatt ccggcagacc actgcctgca tgatgttcag
    40081 gatatgagcg ggataaacca cccgaaagct gatctggcaa aaggccagta cgggactgtc
    40141 ggtcaggggt tgcatattgc caaaaaactg cttccctata tcccacagaa tgccgggata
    40201 cttctggtgc cctgttgtcg tggcgggtca gcattcacca ctggagccga cggctcattc
    40261 agtgaggcca gcggtgcttc cgctgattct tcacgctggg gggccggtaa acctctgtat
    40321 caggacctgg taagccgcac caaagtcgcg ctggcgaaaa acccgaaaaa caaacttctg
    40381 gctgtggtct ggatgcaggg agaggcagac cttgcctcag gaagccagca gcataatagt
    40441 ttattcacgg ccatggttca gcagttcaga actgacctgg ctccgcttgc tgcgcagtgc
    40501 gtgagtggaa atgccaccac ggtgccgtgg atttgcggtg ataccacgta ttactggaaa
    40561 aacgctggca ccgataaata tgaggcagta tacggtggct acaaaggcaa ggaagcacag
    40621 aaaattttct ttgtaccgtt cctgacggat gagaatggac agagcacgcc aacgaatgct
    40681 ccggcggaag acccggatat tgtggctgtc gggtattacg gtgcagcatc ccgaacccag
    40741 ggcagttttg tttcgacaca gcgtgacagc catttcagct catgggcacg caggggcgtc
    40801 atttcagacc gtctggcctc agctattatg ctccatgcag ggcgcacggc tgaactgatg
    40861 cgcgggcaga ccgtgacacc accggatgag aagccgtcac ctgatacacc atcaaaaccg
    40921 tccacgccac ccgcagacac cacgacgatg agtacgctat ttgcttaccg ggcatctgag
    40981 tctgaaggac ggttgacacc acagggttgg gctgccggag gtggcaaggc tcaaatcgtg
    41041 gatgatgccg gagccagcgg gggtaaggca atgaaactga ccaaggaaac aggaagagcc
    41101 ccctggtacc ttgagcatga tgctggtaat ggtgcggacc tgctgggtaa aggtggtctt
    41161 gtcagttgtc gttttaaact cgatggcgcg cttacggcta atcagtacgc actggcgctg
    41221 tactggccgg tttctgccct gccgcaaggg gttactctgg aaggaaatgc cggtcataac
    41281 ctgctggcat cgttctacgt acagagcgat gccacagacc ttaacgtgat gtatcacaaa
    41341 ggaaacacgg cgcagaacac gaagctgggg tcattcggcg catttaataa cgaatggcat
    41401 acgctgggct tccgttttgc cggtaacaac agtattgagg tgacgccggt cattgatggt
    41461 aaggacggga caccattcat gctgtcgcag tctccggtcg gcacgtttac ggcagacaag
    41521 ttgcgcgtga ccgatatcac taaaaatgcg acttacccgg tactgattga cagtatcgtg
    41581 gtggaagtga ataacgcgta agcaggataa aaaaatgccg ccggtaaggg aaaacaagaa
    41641 accggcggca aaaagatcgt tatatccaaa gcaaaaaatg cagtgcacct ttttacctaa
    41701 cagatattac cggtgtcaac accatgtcaa taattgggag ggataatgac atttttacag
    41761 cttattttat tgtatttctg cacggcagta tgtgcgttat atctggtgag tggtggttat
    41821 aaggccatca gaaattatgt tcgccgcaaa attgaggacg cagcagctga aaaactcagt
    41881 aaaacagcgc aggtaactcc gctcccaaaa gacccgaccc agccctgatt gcagggcttt
    41941 attcccgcat aaggaggata tacatgccag aaattaaagg caccgtcact gaagacctgg
    42001 taaaacaggc actgtattcc ggtgaagtaa ataagttgct gaaagcgcag gtccgcaagg
    42061 attttgaggc acaaatcgac acttacgttg atgaagtgct ggcaaaactt attgggccag
    42121 cagttgcggc cagtgatgct gataatgagc cgaaaaccga cgcgcagcca gagcaaccag
    42181 acccggctca gcccggaact gacagtacca tgatgtaaca atacaaaccg gtggtgtcct
    42241 ctgccggttt gacactaatg aaaacatctt tgccgctatc cagccatatg taatcgctgg
    42301 atagtatttc gtattcagaa atatagctgc ttatggagca gtcaatcctg cttccccatc
    42361 cgggcaggca aatgaaccgg tatcattagt ccgattcacg cttttgtaga atcgttccca
    42421 gaatgctata cagctcatta gcctgttgct ccatcattga gacggaggct atacggtgca
    42481 aagtgatttc aggaggtggc acacctccat cttcttgctg tggaggaaaa acataagatt
    42541 taataaaaaa gttgaagcat atgaactctc ccgcactcga actgtatttg caggcatctg
    42601 aataatcgga aaaataatca ttatagattt ccgcattttc aatcattttt cgatttctcg
    42661 tcgttacatg attgtccata gcagcctctt acgctggcaa ttaaccaatt gacttctata
    42721 aattagttga tttgtttgct ttgcggtatt caaatgtaca atcattcagc acttatgcgt
    42781 tacagcaccg tttaaaatta gtgatcaaac tcataaattt tcgctaatga catttcgtgt
    42841 tatttgttgt atcaattaaa taaatcaaat cgatcgtttt tgtcgatcga ttgagtgata
    42901 aagatatcaa caacaaagca ggaggttatg cggccatgta tcgaaacata cggtgccgac
    42961 attgtaacaa gctactggca cgagccagct tcagttatct ggaagttaaa tgtccgcgct
    43021 gtaaaaccct caatcaaatc acatctcaga gcgccacaga gcaccccaca tacacaagga
    43081 aatcctgtcg tggggaacag aaagcaagtt acatcccgta tcatcagcac gcctgaactt
    43141 attcactata acgacaatat cgtcggttat ggctcccgtg agttacgggt tgagacaata
    43201 agttgctggc tggcccggct ggtcgtcgtc aataaacatt acagccatcg tttcgtaaat
    43261 aattcctacc ttcatctggg catattttca gaacgggagc tggttggcgt aatgcaatgg
    43321 ggttatgccc ttaatccaaa caatggtgca cgcgtcgtaa ctggcacgca gaaccgcgaa
    43381 tatatggagc ttaaccggtt atggctgcat gactgtatgc cgagaaactc cgaatcaagg
    43441 gcaatcagtt acgcgctcaa actaatcaga caactttatc cgcaggtgca gtgggttcag
    43501 tcgtttgcag atgagcgttg tggctgtctt ggtgtcgtgt accaggcaag caattttgat
    43561 tatgtaggca gtcatgaaac aacattctat gaacttgatg gcgagtggta tcacgagatt
    43621 tgcaagaatg caatcaaacg aggaggacag cgaggtgaac atctgcggac taatatcgac
    43681 agagccagtg ttcataaatt ccgtcagttt cgttacatcc ggtttctgga caagagagcc
    43741 agaaaacgcc ttaacacaaa gctattcaaa gtccagcctt acccaaaacc acaaacagtt
    43801 aaaactggtt tgaaagagag tgaatgaacg cttaaaaact cattctaaaa ctcaaaagaa
    43861 aagcgccgat ttagtatcaa ataagttgcg tatcggcgtt tttcagcggt atcaaatgaa
    43921 atgcaaatcg gtatcaaaaa aatcgccgcg ctataatttg tcgccgtcag tacagcatgt
    43981 accggaactt taggtttttt tcgttgagcg tcgcgaatag cttcactgtg tggacgtgct
    44041 gcatgaacag ggcggctgtc taaaatcatc aaggtctaaa tacactatga ggttgattag
    44101 gggttggtac aagcccaact aaccgaatca actatgagga tgattcctag aacatccgaa
    44161 tcaactacct gtaaaaacag gacaatatca ggtgaatgac tatgcaattt aacggtgttt
    44221 ttccaagtat ctatgcaatc ctacacaaat tctatgcagt atcggtatag tctcttttta
    44281 gcataactta ttgaaatata taaagaatct gtgaagtctc ttttcagggt aaactgtatc
    44341 gtgttagtcg tccctctcca gacactacat atagtgctat atacagggct atataacgat
    44401 atatagatag ttggtcacat ggtgtaacta tcaagaaact taacgatttc ctacgtgaaa
    44461 gttagaaagt gatggttata gttaccctcc tgaatcttca tagcgctcta ttaaccagtc
    44521 tcataaggaa gtccttttga gacactttca acgtaacggt agaatgcacc ctcaagtagt
    44581 ctcaaaaggt tgtttttgga ttatgagaca gctaa
//
LOCUS       DAJTXQ010000016        43714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000016 DAJTXQ010000000
VERSION     DAJTXQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.016"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..559)
                     /locus_tag="OO515_000878"
     CDS             complement(<1..559)
                     /locus_tag="OO515_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6574883.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO515_000879"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO515_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6574884.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO515_000880"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO515_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6574885.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO515_000881"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO515_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6574886.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="OO515_000882"
     CDS             complement(4001..6322)
                     /locus_tag="OO515_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6574887.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO515_000883"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO515_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6574888.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="OO515_000884"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="OO515_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6574889.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="OO515_000885"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="OO515_000885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6574890.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO515_000886"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO515_000886"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6574891.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="OO515_000887"
     CDS             11268..11582
                     /locus_tag="OO515_000887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6574892.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="OO515_000888"
     CDS             11595..12968
                     /locus_tag="OO515_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6574893.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO515_000889"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO515_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6574894.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO515_000890"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO515_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6574895.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO515_000891"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO515_000891"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6574896.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO515_000892"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO515_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6574897.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="OO515_000893"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="OO515_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6574898.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO515_000894"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO515_000894"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6574899.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="OO515_000895"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="OO515_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6574900.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="OO515_000896"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="OO515_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="OO515_000897"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="OO515_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6574901.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="OO515_000898"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="OO515_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6574902.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="OO515_000899"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="OO515_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6574903.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="OO515_000900"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="OO515_000900"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6574904.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="OO515_000901"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="OO515_000901"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6574905.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="OO515_000902"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="OO515_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6574906.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="OO515_000903"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="OO515_000903"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6574907.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="OO515_000904"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="OO515_000904"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6574908.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="OO515_000905"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="OO515_000905"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6574909.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="OO515_000906"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="OO515_000906"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6574910.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="OO515_000907"
     CDS             31280..32185
                     /locus_tag="OO515_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574911.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="OO515_000908"
     CDS             32202..32564
                     /locus_tag="OO515_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6574912.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="OO515_000909"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="OO515_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="OO515_000910"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="OO515_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="OO515_000911"
     CDS             35034..35912
                     /locus_tag="OO515_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6574913.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="OO515_000912"
     CDS             35923..36276
                     /locus_tag="OO515_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6574914.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="OO515_000913"
     CDS             36288..37592
                     /locus_tag="OO515_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6574915.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="OO515_000914"
     CDS             37604..38689
                     /locus_tag="OO515_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6574916.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO515_000915"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO515_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6574917.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO515_000916"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO515_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6574918.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO515_000917"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO515_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO515_000918"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO515_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6574919.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43714)
                     /gene="frlA"
                     /locus_tag="OO515_000919"
     CDS             complement(42378..>43714)
                     /gene="frlA"
                     /locus_tag="OO515_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6574920.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10158 a        11126 c        11988 g        10442 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcttggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgactgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcatgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccca
//
LOCUS       DAJTXQ010000017        43479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000017 DAJTXQ010000000
VERSION     DAJTXQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.017"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..77)
                     /locus_tag="OO515_000920"
     tRNA            complement(1..77)
                     /locus_tag="OO515_000920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(81..173)
                     /locus_tag="OO515_000921"
     tRNA            complement(81..173)
                     /locus_tag="OO515_000921"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Ser,seq:gct)
     gene            complement(489..674)
                     /gene="csrA"
                     /locus_tag="OO515_000922"
     CDS             complement(489..674)
                     /gene="csrA"
                     /locus_tag="OO515_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6574921.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OO515_000923"
     CDS             complement(909..3539)
                     /gene="alaS"
                     /locus_tag="OO515_000923"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6574922.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OO515_000924"
     CDS             complement(3667..4167)
                     /gene="recX"
                     /locus_tag="OO515_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6574923.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OO515_000925"
     CDS             complement(4395..5456)
                     /gene="recA"
                     /locus_tag="OO515_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6574924.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OO515_000926"
     CDS             complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="OO515_000926"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6574925.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OO515_000927"
     CDS             complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="OO515_000927"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6574926.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7519..8082
                     /gene="srlA"
                     /locus_tag="OO515_000928"
                     /gene_synonym="gutA"
     CDS             7519..8082
                     /gene="srlA"
                     /locus_tag="OO515_000928"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6574927.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8079..9038
                     /gene="srlE"
                     /locus_tag="OO515_000929"
                     /gene_synonym="gutE"
     CDS             8079..9038
                     /gene="srlE"
                     /locus_tag="OO515_000929"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6574928.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9049..9420
                     /gene="srlB"
                     /locus_tag="OO515_000930"
                     /gene_synonym="gutB"
     CDS             9049..9420
                     /gene="srlB"
                     /locus_tag="OO515_000930"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6574929.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9424..10192
                     /gene="srlD"
                     /locus_tag="OO515_000931"
                     /pseudo
     CDS             9424..10192
                     /gene="srlD"
                     /locus_tag="OO515_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10298..10657
                     /gene="gutM"
                     /locus_tag="OO515_000932"
     CDS             10298..10657
                     /gene="gutM"
                     /locus_tag="OO515_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6574930.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10724..11497
                     /gene="srlR"
                     /locus_tag="OO515_000933"
                     /gene_synonym="gutR"
     CDS             10724..11497
                     /gene="srlR"
                     /locus_tag="OO515_000933"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6574931.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11490..12455
                     /gene="gutQ"
                     /locus_tag="OO515_000934"
     CDS             11490..12455
                     /gene="gutQ"
                     /locus_tag="OO515_000934"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6574932.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OO515_000935"
     CDS             complement(12452..13966)
                     /gene="norR"
                     /locus_tag="OO515_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6574933.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14153..15592
                     /gene="norV"
                     /locus_tag="OO515_000936"
                     /pseudo
     CDS             14153..15592
                     /gene="norV"
                     /locus_tag="OO515_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
     gene            15589..16722
                     /gene="norW"
                     /locus_tag="OO515_000937"
     CDS             15589..16722
                     /gene="norW"
                     /locus_tag="OO515_000937"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6574934.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OO515_000938"
     CDS             complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="OO515_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6574935.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OO515_000939"
     CDS             complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="OO515_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6574936.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OO515_000940"
     CDS             complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="OO515_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6574937.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIT
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21200..22657
                     /gene="ascF"
                     /locus_tag="OO515_000941"
     CDS             21200..22657
                     /gene="ascF"
                     /locus_tag="OO515_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6574938.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22666..24090
                     /gene="ascB"
                     /locus_tag="OO515_000942"
     CDS             22666..24090
                     /gene="ascB"
                     /locus_tag="OO515_000942"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6574939.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24204..24623)
                     /locus_tag="OO515_000943"
     CDS             complement(24204..24623)
                     /locus_tag="OO515_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6574940.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24620..24931)
                     /locus_tag="OO515_000944"
     CDS             complement(24620..24931)
                     /locus_tag="OO515_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6574941.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25093..25866)
                     /locus_tag="OO515_000945"
     CDS             complement(25093..25866)
                     /locus_tag="OO515_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574942.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OO515_000946"
     CDS             complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="OO515_000946"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6574943.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OO515_000947"
     CDS             complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="OO515_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6574944.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OO515_000948"
     CDS             complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="OO515_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6574945.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OO515_000949"
     CDS             complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="OO515_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6574946.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OO515_000950"
     CDS             complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="OO515_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6574947.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OO515_000951"
     CDS             complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="OO515_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6574948.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OO515_000952"
     CDS             complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="OO515_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6574949.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OO515_000953"
     CDS             complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="OO515_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6574950.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OO515_000954"
     CDS             complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="OO515_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6574951.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33953..34303
                     /gene="hypA"
                     /locus_tag="OO515_000955"
     CDS             33953..34303
                     /gene="hypA"
                     /locus_tag="OO515_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6574952.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34307..35179
                     /gene="hypB"
                     /locus_tag="OO515_000956"
     CDS             34307..35179
                     /gene="hypB"
                     /locus_tag="OO515_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6574953.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35170..35442
                     /gene="hypC"
                     /locus_tag="OO515_000957"
     CDS             35170..35442
                     /gene="hypC"
                     /locus_tag="OO515_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6574954.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35442..36563
                     /gene="hypD"
                     /locus_tag="OO515_000958"
     CDS             35442..36563
                     /gene="hypD"
                     /locus_tag="OO515_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6574955.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36560..37570
                     /gene="hypE"
                     /locus_tag="OO515_000959"
     CDS             36560..37570
                     /gene="hypE"
                     /locus_tag="OO515_000959"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6574956.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37644..39721
                     /gene="flhA"
                     /locus_tag="OO515_000960"
                     /pseudo
     CDS             37644..39721
                     /gene="flhA"
                     /locus_tag="OO515_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39758..40111)
                     /locus_tag="OO515_000961"
     CDS             complement(39758..40111)
                     /locus_tag="OO515_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6574957.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40188..40322)
                     /locus_tag="OO515_000962"
     CDS             complement(40188..40322)
                     /locus_tag="OO515_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574958.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40398..42959
                     /gene="mutS"
                     /locus_tag="OO515_000963"
     CDS             40398..42959
                     /gene="mutS"
                     /locus_tag="OO515_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6574959.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43065..43479
                     /locus_tag="OO515_000964"
                     /pseudo
     CDS             43065..43479
                     /locus_tag="OO515_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10294 a        11937 c        11327 g         9921 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga tgcagctttt
      121 gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc accacacgcc
      181 tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt
      241 actttctgag acttataagt caaacaattt ttcccacact tttatcgttt gcacacttca
      301 cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa aacggaggtc
      361 tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg
      421 aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa agatgagacg
      481 cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg gtagatctct
      541 tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg gttgcccttt
      601 acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc aactcgacga
      661 gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc
      721 ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta catgtaagca
      781 gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact tcaacctgac
      841 tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg cattgcctga
      901 cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa cgctgcaggt
      961 aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc acccttgccg
     1021 cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc tgtgacgtcc
     1081 ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag cacgataatt
     1141 gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt cggctcaaca
     1201 ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt actggaaaga
     1261 tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt ttctaactga
     1321 cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc gcctttcagc
     1381 agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat agcgccttct
     1441 ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga gatgatgcgg
     1501 aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc ggtggagaaa
     1561 tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag cgccatcgca
     1621 cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa attgcgacga
     1681 atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat cgcttcgttg
     1741 tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg cgatacatga
     1801 gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg attcagacga
     1861 atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac tttcagagaa
     1921 cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt atcttccacc
     1981 gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc gccggattcc
     2041 gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt gatggcatca
     2101 accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag atggtcatag
     2161 cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc accaaagccg
     2221 ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc agcttcgtca
     2281 actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa gccataggtg
     2341 tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt tgccagctct
     2401 tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc ttcagtcttc
     2461 agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga gcccataacg
     2521 tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag catattgccg
     2581 tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc attggacggc
     2641 attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc
     2701 gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat caacgtgcgg
     2761 aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat acgctccaga
     2821 cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc tgcctggcgg
     2881 ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga
     2941 cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg
     3001 ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg cgcacctttg
     3061 ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc ccagatttcg
     3121 taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg cagggcaaac
     3181 catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt gaaatagtcg
     3241 ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt gtaaccgaca
     3301 ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt ggtagcgcgg
     3361 gaataattac gcttgtcgag tccaaggaac acatccttga actggttcat cccggcgttg
     3421 gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac tacctgatgt
     3481 cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct cttgctcata
     3541 attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg
     3601 ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt aaaataccgt
     3661 atgcgttcag tcggcaaaat ttagccaaat atcctggata tcttccatca gatagccacg
     3721 atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca aaggttcacc
     3781 atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac attcacgcat
     3841 cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac gaatacgtgc
     3901 aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc ggctgtcatc
     3961 gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg ccgtagcatc
     4021 aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc gcagttcttg
     4081 ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc gagcatatgc
     4141 cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa agcaaaaggg
     4201 ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga gtgtgcagcc
     4261 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc tgttcccctc
     4321 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4381 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4441 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4501 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     4561 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     4621 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     4681 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     4741 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     4801 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     4861 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     4921 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     4981 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5041 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5101 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5161 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5221 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5281 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5341 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5401 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5461 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     5521 tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     5581 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
     5641 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     5701 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     5761 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     5821 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     5881 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     5941 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6001 ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc aaaaatttat
     6061 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6121 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6181 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6241 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6301 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6361 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6421 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6481 tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     6541 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     6601 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     6661 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     6721 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     6781 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     6841 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     6901 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     6961 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7021 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt cgatgaactg
     7081 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgaggctc
     7141 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7201 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7261 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
     7321 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
     7381 gctatccttt tattcaaact ttcaaattaa aatatttatc tttcattttg cgatcaaaat
     7441 aacactttca aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
     7501 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
     7561 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
     7621 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
     7681 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
     7741 ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat gccggaaaag
     7801 tacaaaccca gctactacgc ggcagcctct tatagctgcc actcaatgaa tggcctcttt
     7861 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
     7921 ctgaacctgc cacttggccc actggcggtg agctatctgc tggttggtct ggtcaccaac
     7981 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
     8041 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
     8101 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
     8161 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     8221 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     8281 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     8341 ttaatatcca ctcgacgggc aagtccagtc cgctggcgca gtacattgtg gaagatattt
     8401 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     8461 cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc gatggtttac
     8521 tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg
     8581 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc tcggcgctca
     8641 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
     8701 cgagccatcc actaggtctg gtcatgctgg cgctcatctg ctccttcccg ctgctttcac
     8761 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     8821 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     8881 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     8941 ttcgcgtcgg tgtcccttct gtactggtga gccacttttt aaccggcgca ccgactgtac
     9001 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     9061 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     9121 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     9181 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
     9241 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     9301 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     9361 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     9421 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     9481 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     9541 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta tagttttggt
     9601 gctgacgcca ccagcgagca aagcgtcctg gcgctctctc gtggggtaga tgaaatcttt
     9661 gggcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac
     9721 ttccagctcg gcgattttga ccgtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
     9781 ttctcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
     9841 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
     9901 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
     9961 gctcggtaac ctgctgaaat cgcctatgtt ccagtcactg ttaccccaat acgcgaccaa
    10021 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10081 cggctgcgat tatcaggatg tgctgaatat gctgctgctc tatgccagtc ctaaggcgtc
    10141 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttgt aatcaacagc
    10201 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    10261 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    10321 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    10381 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    10441 ctttaaacca cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgtcgacac
    10501 cttgtttatg aaaggactga ccgtcttcgc acgaccgcaa aaaattcccg caattaccgg
    10561 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    10621 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    10681 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    10741 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    10801 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    10861 aggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    10921 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    10981 tagttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    11041 ggttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagtc tgcatatcgt
    11101 caatgcgcta tctgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11161 taaaaaatcg gcctcatttc acggacagct ggcagagaat gccttcgaac atttcagttt
    11221 cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacctttaa
    11281 cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    11341 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    11401 taagctgatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    11461 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    11521 agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    11581 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    11641 gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacatcgg
    11701 ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg
    11761 atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc
    11821 gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    11881 gcctggcggc aaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    11941 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    12001 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccca gccggggcac
    12061 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12121 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12181 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12241 tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga
    12301 caggcggcac cacgttacag gcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    12361 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaatta
    12421 acctgcagga tttctatcag gccgggatta tttaatcctt cattcccaga cgtttcgcca
    12481 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttatgat
    12541 gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    12601 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa
    12661 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    12721 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    12781 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagccccagc cgcaaacgac
    12841 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    12901 gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca
    12961 tcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13021 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13081 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag agtgccgtta tccgccattt
    13141 caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    13201 actcactttc cgccacactt tccggcagtg cagcacagtt gagatagacc agcggattca
    13261 ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    13321 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    13381 gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg
    13441 catcgcctgg catcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    13501 ctcccgccgc cagcgcagca atcagccgta gctcttcgtc gctgaaaaca tcgaactgat
    13561 cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca
    13621 gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat
    13681 cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca
    13741 gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga
    13801 tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca
    13861 gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    13921 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    13981 caatttgact atagatattg tcatatcgac catttgattg atagtcattt tgactactca
    14041 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgtaat
    14101 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    14161 tgtggtgaaa aataacattc attaggttgg tcaacgtgac tgggaagtgc gtgattttca
    14221 cggtacggaa tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga
    14281 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    14341 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    14401 ccacgctggg gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac
    14461 tgccaacgct atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt
    14521 ggtgaaaact ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    14581 gccaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    14641 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    14701 tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag
    14761 ccgcctggta acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    14821 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    14881 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    14941 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    15001 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    15061 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    15121 cgccggtctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    15181 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    15241 gggtttcgaa atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga
    15301 gttatgccgt gaacatggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    15361 gagcacggtg aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg
    15421 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    15481 gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga
    15541 atgctctctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    15601 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    15661 gacgccagta ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg
    15721 gacctcagcc atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg
    15781 ggtgaatttg ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc
    15841 gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    15901 ctggcaactg gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    15961 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    16021 ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    16081 aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa
    16141 gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    16201 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt
    16261 agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca
    16321 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    16381 aatgacgata tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc
    16441 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    16501 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    16561 gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg
    16621 gtggctcgcg gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg
    16681 atgaaagagg catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa
    16741 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccag
    16801 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    16861 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    16921 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    16981 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    17041 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    17101 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg
    17161 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg
    17221 aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    17281 cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg
    17341 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    17401 ctgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    17461 gccacggctg cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    17521 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct
    17581 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact
    17641 gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca
    17701 gattcatttc acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
    17761 attgcggggt aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    17821 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    17881 ccaggcagaa ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa
    17941 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    18001 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    18061 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    18121 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    18181 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    18241 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    18301 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    18361 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    18421 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    18481 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    18541 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    18601 cacggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    18661 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    18721 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    18781 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cggatggtga
    18841 actcagtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    18901 gcggcgggca gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga
    18961 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    19021 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    19081 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    19141 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    19201 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    19261 catcagcgcc gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac
    19321 acaaatcagc gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    19381 gcacaggtca catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct
    19441 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    19501 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    19561 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    19621 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    19681 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacagca
    19741 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    19801 cagttaatgc gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga
    19861 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    19921 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    19981 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    20041 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    20101 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    20161 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    20221 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    20281 accgttagtg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    20341 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    20401 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    20461 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    20521 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    20581 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    20641 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    20701 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt
    20761 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    20821 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    20881 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    20941 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    21001 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    21061 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    21121 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    21181 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    21241 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt
    21301 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    21361 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    21421 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    21481 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    21541 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    21601 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    21661 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    21721 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    21781 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    21841 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    21901 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    21961 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    22021 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    22081 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    22141 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    22201 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    22261 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    22321 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    22381 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    22441 agcgccaggg ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    22501 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    22561 cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt
    22621 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    22681 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    22741 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    22801 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    22861 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    22921 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    22981 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    23041 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    23101 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt
    23161 gaagcatttg atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg
    23221 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    23281 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    23341 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    23401 agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt
    23461 atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa
    23521 ggggtaacca tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    23581 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc
    23641 agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    23701 tggggtattg atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag
    23761 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    23821 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    23881 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc
    23941 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    24001 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    24061 attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct
    24121 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    24181 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    24241 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    24301 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    24361 agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca
    24421 aactcttggt tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga
    24481 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    24541 tctgagtcac tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    24601 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat
    24661 actcggcgtg attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg
    24721 caatcaccct taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa
    24781 aattgtcgag gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct
    24841 taataacccg atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact
    24901 cttcaaatgg cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt
    24961 aaacagccac atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc
    25021 aagttcacaa tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca
    25081 ctctgcaaaa agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg
    25141 ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag
    25201 tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca
    25261 gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc
    25321 ggaatcaaac tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc
    25381 atctacgcca gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc
    25441 ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact
    25501 tggtaaccag ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg
    25561 ccctttgccc gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg
    25621 atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc
    25681 agcaacgccc acttttctaa aacgccaggt ttgctcggca tcataaggca tggtcaggag
    25741 aggtttcagc gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa
    25801 gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat
    25861 attcatttcg ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa
    25921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    25981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    26041 ttcgccaatg tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt
    26101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    26161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    26221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    26281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    26341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    26401 gaatatcatg cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg
    26461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    26521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    26581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    26641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    26701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    26761 tcatcggata cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg
    26821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    26881 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    26941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc
    27001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    27061 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg
    27121 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac caccgctgtt
    27181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    27241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    27301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    27361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga
    27421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    27481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    27541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    27601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    27661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg
    27721 cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt
    27781 tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    27841 aagatgcagc gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca
    27901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc
    27961 gggttctgct ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    28021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    28081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    28141 actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    28201 gggtccaagc taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag
    28261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    28321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    28381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta
    28441 tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga
    28501 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg
    28561 acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca
    28621 acgttactga aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt
    28681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    28741 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    28801 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag
    28861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg
    28921 agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    28981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg
    29041 gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    29101 taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag
    29161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    29221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    29281 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    29341 ttacgcagcg gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc
    29401 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    29461 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    29521 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    29581 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    29641 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    29701 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    29761 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    29821 ctaaaaatgc aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac
    29881 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    29941 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    30001 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    30061 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    30121 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    30181 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    30241 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    30301 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    30361 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    30421 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    30481 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    30541 aggcatccgg gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca
    30601 acacacccgg cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    30661 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    30721 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    30781 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    30841 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    30901 agcggtaatc accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga
    30961 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    31021 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    31081 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    31141 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    31201 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    31261 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    31321 ggcaacaaac gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc
    31381 ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    31441 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    31501 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    31561 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    31621 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    31681 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    31741 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    31801 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    31861 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    31921 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    31981 ttgcatacgc atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca
    32041 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    32101 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    32161 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    32221 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    32281 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    32341 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    32401 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    32461 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    32521 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    32581 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    32641 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    32701 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    32761 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    32821 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    32881 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    32941 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    33001 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    33061 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    33121 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    33181 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    33241 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    33301 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    33361 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    33421 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    33481 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    33541 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    33601 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    33661 gtagatgtgc cactggtcct gtaggcgaca atgccgctgt gcgatgtaat cggctttctc
    33721 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    33781 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    33841 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    33901 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    33961 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    34021 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    34081 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    34141 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    34201 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    34261 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    34321 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    34381 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    34441 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    34501 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    34561 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    34621 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    34681 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    34741 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    34801 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    34861 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    34921 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    34981 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    35041 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    35101 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    35161 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    35221 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    35281 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    35341 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    35401 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    35461 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    35521 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    35581 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    35641 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    35701 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    35761 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    35821 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    35881 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    35941 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    36001 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    36061 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    36121 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    36181 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    36241 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    36301 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    36361 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    36421 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    36481 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    36541 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    36601 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    36661 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    36721 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    36781 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    36841 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    36901 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    36961 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    37021 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    37081 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    37141 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    37201 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    37261 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    37321 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    37381 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    37441 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    37501 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    37561 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    37621 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    37681 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    37741 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    37801 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata
    37861 tgaagacgaa actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac
    37921 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct
    37981 ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
    38041 tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct
    38101 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa
    38161 caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat
    38221 taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
    38281 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
    38341 caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag
    38401 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
    38461 gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta
    38521 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
    38581 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct
    38641 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
    38701 ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa
    38761 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
    38821 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
    38881 gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat
    38941 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
    39001 tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga
    39061 taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
    39121 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
    39181 ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga
    39241 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
    39301 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
    39361 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
    39421 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
    39481 cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
    39541 tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct
    39601 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
    39661 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
    39721 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac
    39781 gatatttttc cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta
    39841 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    39901 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc
    39961 cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg
    40021 gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    40081 gtttttcacg agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta
    40141 tattacaact taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac
    40201 gcaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc
    40261 ggaaccagca tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc
    40321 atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg
    40381 gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc
    40441 agcagtatct caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg
    40501 atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac
    40561 tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg
    40621 cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac
    40681 aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta
    40741 cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg
    40801 ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt
    40861 ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc
    40921 cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg
    40981 gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc
    41041 tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt
    41101 gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata
    41161 ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc
    41221 gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc
    41281 tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag
    41341 tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca
    41401 ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc
    41461 tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac
    41521 tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg
    41581 agaagatggg cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac
    41641 cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg
    41701 agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc
    41761 gtgaacgtac cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca
    41821 ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc
    41881 tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca
    41941 cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc
    42001 tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc
    42061 tggtgaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata
    42121 aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc
    42181 catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc
    42241 cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct
    42301 acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta
    42361 cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga
    42421 ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga
    42481 tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc
    42541 tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc
    42601 taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag cacggcgaca
    42661 ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac ggtctggcgg
    42721 ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa aaactgcgtg
    42781 agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg cagatgtctt
    42841 tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg
    42901 attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat
    42961 aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa
    43021 aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct
    43081 atcaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg
    43141 aatatcagtt attacaatcc cgttaacatc aactctcttt ttaccctgaa accgacttac
    43201 ttatttccac aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc
    43261 taaaccaacc ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat
    43321 tcgaaactgg cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa
    43381 atgattcaga gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata
    43441 ttattgaaat cattaatgac aacataaaat atgccatcg
//
LOCUS       DAJTXQ010000018        40826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000018 DAJTXQ010000000
VERSION     DAJTXQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.018"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..687
                     /locus_tag="OO515_000965"
     CDS             <1..687
                     /locus_tag="OO515_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCR6574960.1"
                     /translation="RREYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYY
                     DPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMAPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
     gene            689..925
                     /locus_tag="OO515_000966"
     CDS             689..925
                     /locus_tag="OO515_000966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6574961.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1098..1773)
                     /locus_tag="OO515_000967"
                     /pseudo
     CDS             complement(1098..1773)
                     /locus_tag="OO515_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1918..2526)
                     /locus_tag="OO515_000968"
     CDS             complement(1918..2526)
                     /locus_tag="OO515_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6574962.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2710..3027)
                     /gene="metJ"
                     /locus_tag="OO515_000969"
     CDS             complement(2710..3027)
                     /gene="metJ"
                     /locus_tag="OO515_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6574963.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3304..4464
                     /gene="metB"
                     /locus_tag="OO515_000970"
     CDS             3304..4464
                     /gene="metB"
                     /locus_tag="OO515_000970"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6574964.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4467..6899
                     /gene="metL"
                     /locus_tag="OO515_000971"
     CDS             4467..6899
                     /gene="metL"
                     /locus_tag="OO515_000971"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6574965.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7248..8138
                     /gene="metF"
                     /locus_tag="OO515_000972"
     CDS             7248..8138
                     /gene="metF"
                     /locus_tag="OO515_000972"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6574966.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8467..10647
                     /gene="katG"
                     /locus_tag="OO515_000973"
     CDS             8467..10647
                     /gene="katG"
                     /locus_tag="OO515_000973"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6574967.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10741..11646
                     /gene="yijE"
                     /locus_tag="OO515_000974"
     CDS             10741..11646
                     /gene="yijE"
                     /locus_tag="OO515_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6574968.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11673..12290)
                     /locus_tag="OO515_000975"
     CDS             complement(11673..12290)
                     /locus_tag="OO515_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6574969.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12564..13667)
                     /gene="gldA"
                     /locus_tag="OO515_000976"
     CDS             complement(12564..13667)
                     /gene="gldA"
                     /locus_tag="OO515_000976"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6574970.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13678..14340)
                     /gene="fsa"
                     /locus_tag="OO515_000977"
     CDS             complement(13678..14340)
                     /gene="fsa"
                     /locus_tag="OO515_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6574971.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14352..16853)
                     /gene="ptsP"
                     /locus_tag="OO515_000978"
     CDS             complement(14352..16853)
                     /gene="ptsP"
                     /locus_tag="OO515_000978"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6574972.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17162..18241
                     /locus_tag="OO515_000979"
     CDS             17162..18241
                     /locus_tag="OO515_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6574973.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18256..18576
                     /locus_tag="OO515_000980"
                     /pseudo
     CDS             18256..18576
                     /locus_tag="OO515_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18627..20925
                     /locus_tag="OO515_000981"
                     /pseudo
     CDS             18627..20925
                     /locus_tag="OO515_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20891..21769
                     /locus_tag="OO515_000982"
     CDS             20891..21769
                     /locus_tag="OO515_000982"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6574974.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21771..22112
                     /locus_tag="OO515_000983"
     CDS             21771..22112
                     /locus_tag="OO515_000983"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6574975.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22099..22950)
                     /locus_tag="OO515_000984"
     CDS             complement(22099..22950)
                     /locus_tag="OO515_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6574976.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23165..24898)
                     /locus_tag="OO515_000985"
     CDS             complement(23165..24898)
                     /locus_tag="OO515_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6574977.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25081..27732)
                     /gene="ppc"
                     /locus_tag="OO515_000986"
     CDS             complement(25081..27732)
                     /gene="ppc"
                     /locus_tag="OO515_000986"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6574978.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28084..29235)
                     /gene="argE"
                     /locus_tag="OO515_000987"
     CDS             complement(28084..29235)
                     /gene="argE"
                     /locus_tag="OO515_000987"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6574979.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29389..30393
                     /gene="argC"
                     /locus_tag="OO515_000988"
     CDS             29389..30393
                     /gene="argC"
                     /locus_tag="OO515_000988"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6574980.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30401..31177
                     /gene="argB"
                     /locus_tag="OO515_000989"
     CDS             30401..31177
                     /gene="argB"
                     /locus_tag="OO515_000989"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6574981.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31238..32611
                     /gene="argH"
                     /locus_tag="OO515_000990"
     CDS             31238..32611
                     /gene="argH"
                     /locus_tag="OO515_000990"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6574982.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32878..33795
                     /gene="oxyR"
                     /locus_tag="OO515_000991"
     CDS             32878..33795
                     /gene="oxyR"
                     /locus_tag="OO515_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6574983.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33778..35178)
                     /gene="sthA"
                     /locus_tag="OO515_000992"
     CDS             complement(33778..35178)
                     /gene="sthA"
                     /locus_tag="OO515_000992"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6574984.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35499..36149
                     /gene="fabR"
                     /locus_tag="OO515_000993"
     CDS             35499..36149
                     /gene="fabR"
                     /locus_tag="OO515_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6574985.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36149..36508
                     /locus_tag="OO515_000994"
     CDS             36149..36508
                     /locus_tag="OO515_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6574986.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36548..37648)
                     /gene="trmA"
                     /locus_tag="OO515_000995"
     CDS             complement(36548..37648)
                     /gene="trmA"
                     /locus_tag="OO515_000995"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6574987.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37762..37952
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38017..39861
                     /gene="btuB"
                     /locus_tag="OO515_000996"
     CDS             38017..39861
                     /gene="btuB"
                     /locus_tag="OO515_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6574988.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39806..40663
                     /gene="murI"
                     /locus_tag="OO515_000997"
     CDS             39806..40663
                     /gene="murI"
                     /locus_tag="OO515_000997"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6574989.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9434 a        10168 c        11213 g        10011 t
ORIGIN      
        1 cgccgggaat atgatgaatg gggcaacctg ctgaatgaag agaacccgca tcagctgcag
       61 cagcttattc gtttgccggg gcagcagtat gatgaggagt ccggcctgta ttataaccgc
      121 caccgctatt atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag
      181 gggggatgga acctgtatac atatccgctg agcccggtga atagcatggc tccattagga
      241 ttatatgaat ttaaatcaaa aaatattgat gatattggaa tatttgcatt ggcaatgtgt
      301 aatggagaat caattaacga gaataaagaa tatggtggac taatatgtaa gaagcaaggt
      361 gaatatttcc ccatgaatcc gataagttca aatgataatg atagtgtaga cttgcgaaat
      421 ataaaatgcc ctgaaggttc agagagagta ggcgattatc acactcacgg tttttactct
      481 gacgataaag gaaataaagt aacaaaagaa aatgatgttt atgatagtct aaatttttca
      541 agcaaagatt taacgaattc ttatatgaat ggaatgggaa aaaaagaata cagtagttac
      601 ttgggaacac caaataacac ctatctaaaa tataatccca aagctaaagg gaatggagtt
      661 acaattatca ggcaagggag taattaaaat gaaggcaata caatgttgtt atttccttgc
      721 tttagtctcc agtcatttaa tggctggagt tatgggtgat gataatatat ttttaagtaa
      781 ttcggattca gctgctttat attgtgtggc taaaatattt ccagatggag aaaatatttt
      841 aatatataaa gtaaatataa ttaatgctag ttcaaaaaaa gatgtttttt ttatgaaagc
      901 ccaacatcaa cccacaatga cataatgggg ggcgggggaa tgtcatatgc ctatgaatat
      961 gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg
     1021 gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa
     1081 ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa
     1141 cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg
     1201 ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct
     1261 ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg
     1321 cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac
     1381 cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc
     1441 tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg
     1501 ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag
     1561 cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt
     1621 aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg
     1681 gccgcggaat cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct gaacggactg
     1741 gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata
     1801 tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt
     1861 cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta
     1921 ccacgaaaac agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc
     1981 ttgtggtgaa actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt
     2041 agcctggacc agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg
     2101 cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc
     2161 gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag
     2221 tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt
     2281 ttcaccatgg gcgatccact gcttgagcgg ggggtatttc accgggccga ccgcttcaaa
     2341 aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt
     2401 cgccagttgg atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt
     2461 ttgtggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt
     2521 tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt
     2581 tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat
     2641 tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc
     2701 ttttttgctt tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt
     2761 tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg
     2821 ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg
     2881 caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg
     2941 aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct
     3001 gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag
     3061 accagaccac acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc
     3121 gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct
     3181 atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc
     3241 attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat
     3301 cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag
     3361 tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat
     3421 gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt
     3481 gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg
     3541 attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac
     3601 tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg
     3661 ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa
     3721 ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc
     3781 tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg
     3841 gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat
     3901 ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc
     3961 actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat
     4021 ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg
     4081 caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg
     4141 ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg
     4201 ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg
     4261 ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc
     4321 atgacacatg caggcatggc accagaagcg cgtgctgccg ccgggatctc cgagacgctg
     4381 ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc
     4441 ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc
     4501 gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg
     4561 cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta
     4621 gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc
     4681 atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg
     4741 ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc
     4801 tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat
     4861 ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg
     4921 atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt
     4981 cttacccgtt gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg
     5041 ggtttatcag ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact
     5101 attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg
     5161 tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc
     5221 tgcgtctgga tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta
     5281 ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg
     5341 atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca
     5401 ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac
     5461 tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg
     5521 gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg
     5581 cgctgaaaat cctcgacgaa gcggggttac ctggcgaact gcgcctgcgt caggggctgg
     5641 cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct
     5701 ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc
     5761 tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg
     5821 tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc
     5881 gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat
     5941 ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg
     6001 ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt
     6061 tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc
     6121 agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca
     6181 acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa
     6241 aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca
     6301 ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg
     6361 gcacgctctc ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg
     6421 atcaggcgtg gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag
     6481 acgtgatgcg caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc
     6541 aggtacgcgt ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct
     6601 ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa
     6661 tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg
     6721 tggaagcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg
     6781 ccatcgaaag ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc
     6841 gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat
     6901 ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata
     6961 tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca
     7021 ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt
     7081 ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt
     7141 tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag
     7201 atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc
     7261 acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg
     7321 tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca
     7381 tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg
     7441 gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag
     7501 cggcaccgca tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg
     7561 actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa
     7621 gtggtaagcc agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt
     7681 tcgatatctc cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg
     7741 atttgcttaa tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct
     7801 tcttcgatgt cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg
     7861 tggaaattat tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg
     7921 atatgaccaa cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg
     7981 atgccgaaac ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa
     8041 gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg
     8101 cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat
     8161 cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga
     8221 aaataaggtt gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc
     8281 ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat
     8341 ttgacctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
     8401 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
     8461 acattgatga gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc
     8521 catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca
     8581 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
     8641 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
     8701 aaagccctgc tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt
     8761 ctgtttattc gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc
     8821 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
     8881 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc
     8941 tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc
     9001 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
     9061 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
     9121 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg
     9181 ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa
     9241 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca
     9301 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     9361 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg
     9421 acccagacgc cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg
     9481 gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt
     9541 atcccggatc cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     9601 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     9661 gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg
     9721 aaatctcgct acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg
     9781 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     9841 gattctggtc tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc
     9901 cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc
     9961 gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa
    10021 gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag
    10081 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
    10141 gcgcgtcagg atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt
    10201 ttccgtaact atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa
    10261 gcccagcaac tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta
    10321 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
    10381 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
    10441 tcgaaagagc tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc
    10501 cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc
    10561 agcagcgatg ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg
    10621 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag
    10681 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
    10741 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
    10801 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
    10861 tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
    10921 ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc
    10981 tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg
    11041 atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa
    11101 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
    11161 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
    11221 ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc
    11281 gtggatttat tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg
    11341 gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg
    11401 gcctacagtg cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa
    11461 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
    11521 ttttcctggt ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg
    11581 attgtgatgg cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg
    11641 atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc
    11701 accatgcgcc agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc
    11761 accagcggcg taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg
    11821 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg
    11881 gggcgtgttt tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg
    11941 atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc
    12001 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
    12061 acatcggaac ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata
    12121 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca
    12181 aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
    12241 tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc
    12301 cgtgaaaata agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat
    12361 gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag
    12421 tgccggatgc ggcgtgaacg ccttatctgt cctaaaaaaa aagaaaattc aatatattgc
    12481 agagattgcg taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg
    12541 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
    12601 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
    12661 atggtttcac attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct
    12721 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
    12781 acggtttcga tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat
    12841 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
    12901 actgcgtgcg ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc
    12961 gcttcaatca cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct
    13021 ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg
    13081 gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac
    13141 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg
    13201 attttggtgt cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac
    13261 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
    13321 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg
    13381 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
    13441 agacggtcga tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt
    13501 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
    13561 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
    13621 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
    13681 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
    13741 taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca
    13801 tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat
    13861 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
    13921 tccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
    13981 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
    14041 tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc
    14101 cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat
    14161 ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc
    14221 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
    14281 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
    14341 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
    14401 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
    14461 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat
    14521 tgcgcttcat catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat
    14581 tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag
    14641 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct
    14701 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt
    14761 tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc
    14821 gccagcaggt gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg
    14881 cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg
    14941 ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct
    15001 cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa
    15061 tcgagcgccc acaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta
    15121 tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
    15181 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
    15241 agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc
    15301 agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc
    15361 aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg
    15421 tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg
    15481 tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
    15541 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
    15601 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
    15661 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
    15721 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc
    15781 tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc
    15841 gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata
    15901 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
    15961 gtgccgccac ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg
    16021 ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg
    16081 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
    16141 gcacgttctt gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca
    16201 ctggcgacaa tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga
    16261 cgtaaagatg tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg
    16321 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
    16381 gtggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
    16441 aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg
    16501 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
    16561 acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
    16621 tggtgcgctt cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc
    16681 gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg
    16741 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
    16801 cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
    16861 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
    16921 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
    16981 acgccgtttt gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt
    17041 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
    17101 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
    17161 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
    17221 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
    17281 tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat
    17341 cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat
    17401 tgcagaacgt tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg
    17461 tgcgggcttc tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
    17521 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
    17581 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
    17641 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
    17701 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
    17761 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc
    17821 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
    17881 tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt
    17941 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
    18001 ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
    18061 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
    18121 ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa
    18181 aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta
    18241 attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgcc
    18301 catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg
    18361 aaagtagaaa cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg
    18421 agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
    18481 ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg
    18541 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
    18601 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
    18661 tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
    18721 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
    18781 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
    18841 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
    18901 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
    18961 gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
    19021 tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata
    19081 aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc
    19141 tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
    19201 cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa
    19261 cgatggcggc aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga
    19321 tctctcgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
    19381 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
    19441 tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt
    19501 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
    19561 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg
    19621 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
    19681 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
    19741 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
    19801 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
    19861 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
    19921 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
    19981 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
    20041 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
    20101 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
    20161 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
    20221 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
    20281 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
    20341 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
    20401 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt
    20461 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    20521 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    20581 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    20641 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccccct ggaaggtgaa
    20701 gcgggattac gcaaactggc cgacttctta cgggcgttta cccagcttaa gttgcagcat
    20761 attcagttta acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg cccacaagat
    20821 tatgccgggc tggtggtgcg cgttgccgga tacagcgcct tctttgtcga actgtcgaag
    20881 gagatccagg atgacatcat ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg
    20941 ccgcgatgat gtggcgcgca ttttcaacat tcagcgttat tcactgaatg acggcgaggg
    21001 cattcgtacg gtggtctttt ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc
    21061 ggagtcgatc tccggcaaaa tccagacggt acgcagagag gcgaaatgtc tgcactgtgc
    21121 gaaatgtttg cgtgatgcgg atgaatgccc ctccggggcg tttgaacgga ttggtcgcga
    21181 tatcagcctt gacgctctgg aacgggaagt gatgaaagat gacattttct ttcgcacgtc
    21241 cggcggcggc gtcacgcttt ctggcggcga agtgttaatg caggcggagt ttgctacccg
    21301 ttttttacag cgactgcggc tgtggggtgt gtcatgcgcc attgaaactg ccggagacgc
    21361 gccagcaagc aagctgttac cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa
    21421 aattatggac gcgactcagg cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga
    21481 gaatctgcgt ttgctggtga gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc
    21541 tggtttcacg ctcagcaggg agaatatgca gcaggcgctg gatgtactga tcccgctgaa
    21601 aatcaggcag atccatctgt taccgtttca tcagtacggc gaaccgaaat accgcctgct
    21661 ggggaaaaca tggtcgatga aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat
    21721 gcgcgaaatg gcagaacggg ccggatttca ggttaccgtg ggaggttaaa atggcatacc
    21781 tggtggcagt aaccgcctgc gtaagcggcg tggcgcatac ttatatggcg gcggaacggc
    21841 tggaaaagtt gtgccagtta gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg
    21901 gaacggagaa tcgtttagcg gacgaggata tccgtcgggc ggatgttgct ctgttgatta
    21961 cggatatcga gcttgccggt gccgagcgat ttgaacattg ccgctatgtg caatgcagca
    22021 tctacgcatt cctgcgtgag ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg
    22081 cgccgcagca aacccatctt attctggagt agtcggtttt tctgtcagtt ggctgtggta
    22141 ctgccggcga tattccgacg gcgagcgttc ggtgttttta cgaaacagac ggcagaagta
    22201 gttgctgtcg acaaagccgc aggcgtgcgc cacttctttt accttcaggt catagccttt
    22261 caataacgtc ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc
    22321 ccccgttttt tgaaacaggt gcgagagata gtttggtgaa atatagaatg cctgtgcgac
    22381 agattcgcgg gtaagcgcgg aggcatagcg ttcgtcgata taatcgcgaa tagcttcaaa
    22441 tagtgcctgg ctgcgtgagg cggtctggat ttggctgccg agcaaatcgc ggcagtggct
    22501 aagcaggctg gctacgataa gccttgccgt ttgctgctcc tgcggctgca tttgcatttc
    22561 attgagcgtt tgtagcagaa aagaacctat acgtgggccg cgtcgggcga cgtgttgctt
    22621 cgccagattt tgatattgtt tgccatccca ttgcacgacg ctgaagccga gttgctgttt
    22681 gccaaacagc acgctaaagg tggtagcggg tgttttccat tgtgggaaat tccagccacc
    22741 agcggcaacg tacagcacat cgccgggaac taacgttgct ccagcgccgg tatcaacaaa
    22801 ctcaccttcc agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac
    22861 cgggccgtta ctgctggcaa agtaaatctg gcgcagggac agcgggccgt tgatcaggcg
    22921 ggagagcaga tagctgacgc cgtgatacat gttattccct taggtatgta gacgggaaaa
    22981 cagtaactgg cattattaaa aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg
    23041 taaacattgc ggcagttgta ttgccggatg tggcgtaaac gccttatcca gcctaccgcc
    23101 gcgatctgta ggccggataa gacgcgtcaa gcgtcgcatc cggcaaatag cgcctggctg
    23161 ataattactg attacctacc tggtcgccat acggcagagt gtcgtaatcg atcagcgcgt
    23221 ttttcttgta cgggttacca atccagcggg tagtttcttt gaactgcgga ttcaccactg
    23281 aacgggttgg atcgtaaccg tcgtaagata agcccgccaa atctgaccag gtgtggatca
    23341 gttccgccag gctgtattta cgatcgacat cctgtgagaa atcacgggga tgagtcgctt
    23401 gccatttttc cgacgtccac agcaggaacg gaatggtgta catgtggcgc gtcgggttgt
    23461 cttcgttacg cccctgagtt ttgtgcggcg gcgtgtcgta aacctcttca ccgtggtcag
    23521 agaagtaaac caggaatccg ttcgggtctg ccgctttaaa gtctttaatc aggctggcaa
    23581 ccacatgatc gttatacagg ttagcgttgt cataatcgtt atatgattcc agctcttctg
    23641 cgcttaatcc tggcggaaca tgatcggtat tgccatcaaa cttgccctga ttttccgggt
    23701 agcggtattt gtatttgata tgcgtaccca gcagatgaac gatgatcagt ttcttcggcg
    23761 cagggtcatt cagcacatcc tggaacggct tcagcacgtt ggtgtcgtat tcacgcgcac
    23821 tctgcgtacg ttgctggttc atgtagtact gcttgtcggt ctggcgcgaa aataccgtca
    23881 gcatggtatt gcgggcggtc atcgtctgct ggttggtgat ccagaaggtt ttataacccg
    23941 cctgtttcat catgttcatc agcgacggct gcgtcagata cagatccggg ttcttttcat
    24001 tggcaaaggt cagcgcctgt tgcaggattt caatggtgta cggacgagac gtaactacgt
    24061 tattaaacac ggtcagattc ggatcggttt tatgcagcgc atccagctcc ggcgtggttt
    24121 cacgcggata accgtacaga ctcatgcgtc cgcgctgggt cgactcgcca atcaccagca
    24181 ctaaagtgcg cggttcgtta cccgattcat ctttgaaatt agccagtggc ggcaaggcat
    24241 tattttcatt cagtaacttt gttagcgagt ttagttgctg acgatactga taatagccgg
    24301 tcaggaattg ccacggtgcg gcaggctcca tacgcgaggc caggttatcc aacgttttct
    24361 caaacggctt gtttttgata aacgtattca tggcgatcgg atgcagaatc aagccataaa
    24421 gcagggcaaa agagacaaca taacgccacg gctttggaat atagaccggg cgcaggcgtg
    24481 tccacagcag aactgccacc gccgtatagg ccagcgcgat aagcacaatt ttcaggctga
    24541 aatactggct taaatactcg ctggcttcgt tggtgttggt ttcgaacatc acaaacagaa
    24601 cgctctgcga gaactcctgc ccgtagatga cgtagtagca tagcgccgcc agagaggccg
    24661 cccatagcac cacgccgatt actgcggcaa taattttaat ccgcttcgga aagaggaata
    24721 ccgggatcaa ccacagcgag ctgaataaca gcgagtcgcg aatgccgttg gtgccactat
    24781 aaccactgat gtaaataatg gcctgtagca gagtagagaa aaaccaaaag tagagcagtg
    24841 cccaacccag ggctttccag ctaaaaaggg gtttagcctg gacttctgtg gaatgcatag
    24901 taagaacctg tcttgaaaaa aatatcgccg aatgtaacga caattcctta aggatatctg
    24961 aaggtatatt cagaatttga ataaaatgca gacagaaata tattgaaaac gagggtgtta
    25021 gaacagaagt atttcagaaa accctcgcgc aaaagcacga gggtttgcag aagaggaaga
    25081 ttagccggta ttacgcatac ctgccgcaat cccggcaata gtgaccatta acgcttgttc
    25141 gacgcgagga tccggttcct ggccttcttt ttctgcctgg cgggagcggt gcagcaactc
    25201 ggcctgcaat acgttcagcg ggtcggtgta aatattccgt agctgaatag actctgcaat
    25261 ccacggcaga tcggccatca gatgggaatc gttggcaatc gccagcacca ctttgatgtc
    25321 ttcttcttgc aggttgcgta actctttacc taacggccac agtgctttgt ctaccagacg
    25381 ttgatcatag tattccgcca accacaggtc tgctttggcg aagaccatct ccagcatgcc
    25441 gagacgcgtc gagaagaatg gccaatcgcg gcacatggct tccagctcgc tctgtttgcc
    25501 atcttcgacc actttttgca gcgccgtacc tgcacccagc caggcgggga gcatcaggcg
    25561 gttttgcgtc caggcaaaaa tccacggtat ggcgcgcagc gactcgacgc cgccggttgg
    25621 gcgacgtttc gccggacgtg aacccaacgg cagtttgccc aattcttgtt ccggcgtagc
    25681 ggagcggaag taaggcacaa aatctttgtt ttcacgtacg tagccacggt agagatcgca
    25741 ggagatgact gacagttcat ccataatgcg acgccagctc tctttcggct caggtggtgg
    25801 cagcaggttg gcttccagaa tcgccccggt gtaaagcgac aggctgctga cggtgatttc
    25861 tggcagacca tatttaaagc ggatcatctc gccctgttca gtcacgcgca ggccaccttt
    25921 caggcttcct ggcggttgtg acagcagcgc cgcatgggca ggtgcgccgc cgcgaccaat
    25981 ggaaccgccg cgaccgtgga acaacgtcag ctcaataccc gctttttcgc aggttttgat
    26041 taatgcatcc tgtgcctgat attgcgccca ggaagctgcc atcacgcccg catcttttgc
    26101 tgagtcggaa tagccaatca tcaccatctg tttgccctga atcaggccgc gataccagtc
    26161 gatattgagc agctgggtca tgacatcgtt ggcgttgttc aggtcatcga gggtttcaaa
    26221 cagcggagca accggcatcg caaacccgat acccgcttct ttcagcagca ggtggacagc
    26281 cagtacgtcg gacggcgttt tcgccatcga gatcacgtag gcggcaatgg agccttgcgg
    26341 cgcttcggca atcacctggc aggtatcgag cacttcgcgc gtttcggcgc ttggttgcca
    26401 gttgcgcggc agaagcggac gtttggagtt cagttcgcgg atcaggaacg cctgtttgtc
    26461 ggcctctgac cagctttcgt agtcgccgat accgaggtag cgggtcagct cgcccagcgc
    26521 ttcggtatgg cgggtgctct cctgacggat atcaatacgg accagcggta cgccgaaaca
    26581 tttcacgcgg cgcagggtgt cgagcagatc gccgttggcg ataataccca tgccacacgc
    26641 ctgaagtgac tggtagcaag cgtagagcgg ttcccacagt tcttcgtttt gtgtcagcag
    26701 cccttctggt tttggcagtt cttcgccttt caggcgcgct tccagccatg cctgtgtcgc
    26761 catcaggcga gaacgcaggt ttttcatcag atagcgatac ggttctgcgg caccttcttc
    26821 gccaaccagc gccagcagtt caggggtcgc ttcaaccatc gacagttcag aaaccagaac
    26881 ctgaatatct ttcaggaaca gatcggtggc tttccagcga ctgagtagca ggacgtggcg
    26941 ggtgatatcg gcagtgacgt tcgggttgcc gtcgcggtca ccgcccatcc acgaagtaaa
    27001 acggaccgga acaaattcga cgggcagttt gtagccgagg ttctcttcca gttgttcgtt
    27061 cagttcgcgc aagtaattcg gtacgccttg ccacaggctg ttttctacta cggcaaagcc
    27121 ccatttggct tcatctaccg ggcttggacg cagcttacgg atttcatcag tatgccatga
    27181 ctgggcgatt aactggcgca ggcgacgcat cagctggttg tgttcgtagt cagcgatatc
    27241 tttgttatcg agctgtttta agcaggcgtt cacttccacc attttgtgga tcagtgtacg
    27301 acgggtaatt tcggttgggt gagccgtgag gaccagttcc agcgacagcg attccactgc
    27361 ttttttgatg gtgtcttcgc tcagttccgg ctggtttttc agtttgcgca gggtgcgggc
    27421 gatcacttcc gggttgctgg cagcttcgcc tttcggcgaa atgctgtggt attgctcggc
    27481 ggtgttggcc aggttcagga actgactaaa cgcacgcgca acgggcagca gctcgtcgtt
    27541 cgacaaattt tgtaaggtgg tgagcaactc ctggcggtta gcatcattgc cagcgcgtga
    27601 agatttcgac aacttacgga tagtttctac gcgttcaaga atgtgttctc ccaacgcatc
    27661 cttgatggtt tctcccagca ctttgccgag catactgaca ttactacgca atgcggaata
    27721 ttgttcgttc atattacccc agacacccca tcttatcgtt tgatagccct gtatccttca
    27781 cgtcgcattg gcgcgaatat gctcgggctt tgcttttcgt cgtcttttat aaagccacgt
    27841 aaaagcggtg acgtcaaatg ctgcgaaatc gcttcagcaa acgaataaat agcaggaatt
    27901 tacgccatta aattcacgac gctttaaata agcgtaactt atggaaatgt taaaaaatcg
    27961 ccatacataa caccaaaggt gtaggtcgga taagatgcgc aagcatcgca tccgacatta
    28021 ttgcggcacc ggagtttggc aacggtgccg gatgcggcgc gagcgcctta tccggcctac
    28081 gttttaatgc cagcaaaaat ggtgaattac ctggataatc aactcacggg tgggtttaat
    28141 aaaccgcgtc tccagatatt catcaggctg atgagcttga ttgatggaac ccggaccgag
    28201 caccagcgtc gggcataacg tttgaataaa cggcgcttcg gtacagtaat tcaccacttc
    28261 ggtttttgct ccgagcaatt tctcaaccac ttcaaccagt tgatgattcg gtgggcattc
    28321 atagccaggg atcggcggat gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct
    28381 caccggagcc aatgcatcgt tgagcaaacc attaagttca ttgagtgtca tgctaggcag
    28441 cggacgaata tccatatgca actcacagca agcgcaaata cggttagaag cgtcgccacc
    28501 gtgaatatgc ccgaggttga gcgtagggta tggcacggta aatgcttcgt agtgataacg
    28561 ttctttcagg ttatcgcgca attgcaaaat atgcccgatg gcgtcgtgca ttagttcgat
    28621 agcgttaact ccgcgtgctg gatcgctgga gtgccccgac tggccctgaa tacggatggc
    28681 gttagagata tgacctttat gtgcgcgtac cggttgtagt gacgtcggtt cgccaatgat
    28741 ggcgcaatcc gggcgcaggg cggtagtttc ggcaaaataa cgcgctccgg ccatactggt
    28801 ttcttcatca gcagtcgcca gaatgtagag cggttttttc agtttcgtga cgtcgacatc
    28861 gcgtagcgta tcaaggataa acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta
    28921 aagcttgccg tcatgctccg tcagtgtaaa cggatcgcgc gtccagcgac cgtcatcaaa
    28981 tggcaccgta tcggtatgcc ccgccagcaa caagccgcca gccccctgtc cgatacttgc
    29041 cagcatattg aatttgttgc gagttcctgg cacaggctgc acttccacat tgaagcctaa
    29101 atctttaaac cagtccgcca gcagagtgat taaatctgca ttgctttgat cgagtgcctc
    29161 ttccgtggcg cttattgaag gtgtggcaat cagagcacgg taaatctcga taaatggcgg
    29221 taatttgttt ttcattgttg acacacctct ggtcatgata gtatcaatat tcatgcagta
    29281 tttatgaata aaaatacact aacgttgagc gtaataaaac ccaccagccg taaggtgaat
    29341 gttttacgtt taacctggca accagacata agaaggtgaa tagccccgat gttgaatacg
    29401 ctgattgtgg gtgccagcgg ctacgctggc gcagagctag tgacctatgt aaatcgccat
    29461 ccgcatatga acataaccgc tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta
    29521 atctccgatt tgcatccgca gctaaaaggc atcgttgagc tgccgttgca gccgatgtcg
    29581 gatatcagcg agtttagccc aggggtggac gtagtgtttc tcgccaccgc ccatgaagtt
    29641 agccacgatt tagcgccgca gtttcttgaa gcgggctgcg tggtgttcga cctttccggt
    29701 gcgtttcgtg ttaacgacgc caccttctat gaaaaatatt acggctttac ccatcaatac
    29761 ccggaactgc tggaacaggc agcctacggt ctggcggagt ggtgcggtaa taaattaaaa
    29821 gaagcgaatt tgattgcggt gccaggctgt tatccgacgg cggcacagct ggcgctgaaa
    29881 ccgttgattg atgccgatct tcttgacctc aatcagtggc cggtgatcaa cgccaccagc
    29941 ggcgtgagcg gtgcaggacg taaggcagcg atttcaaata gtttttgtga agttagcctg
    30001 caaccgtatg gcgtctttac tcatcgccat caaccagaga tcgccacaca cctcggtgct
    30061 gacgttatct tcaccccaca tctgggcaat ttcccgcgcg gcattctcga aaccattacc
    30121 tgccgcctga aatcgggtgt gacccaggcg caagtcgcgc aagtgttaca gcaggcgtat
    30181 gcccataaac cgctggtgcg gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt
    30241 gggctgccat tttgcgatat cgggtttgcc gttcagggcg agcatctgat tattgtggcg
    30301 accgaagaca acttactgaa aggcgcggcg gcacaagcgg tacagtgcgc caatattcgt
    30361 ttcggctatg cggaaacgca gtctcttatt taagggtgca atgatgaatc cattaattat
    30421 caaactgggc ggcgtactgc tggatagtga agaggcgctg gaacgtctgt ttagcacact
    30481 ggtgaattat cgtgagtcac atcagcgtcc gctggtgatt gtgcacggcg gcggttgcgt
    30541 ggtggatgag ctgatgaaag ggctgaatct gccggtgaaa aagaaaaacg gcctgcgggt
    30601 gacgcctgct gatcagatag acattatcac cggagcactg gcgggaacgg caaataaaac
    30661 cctgttggca tgggcgaaga aacatcagat tgcggccgta ggtttgtttc tcggtgacgg
    30721 cgacagcgtc aaagtgaccc agcttgatga agagttaggt catgttggac tggcgcagcc
    30781 aggttcgcct aagcttatca actccttgct ggagaacggt tatctgccgg tggtcagctc
    30841 cattggcgta acagacgaag ggcaactgat gaacgtcaat gccgaccagg cggcaacggc
    30901 gctggcggca acgctgggcg cggatctgat tttgctctcc gacgtcagcg gcattctcga
    30961 cggcaaaggg caacgcattg ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca
    31021 gggcattatt actgacggca tgatagtgaa agtgaacgcg gcgctggatg cggcccgcac
    31081 gctgggccgt ccggtagata tcgcctcctg gcgtcatgcg gagcagcttc cggcactgtt
    31141 taacggtatg ccgatgggta cgcggatttt agcttaagtt ttgttggccg gaggcgcagc
    31201 tttccggcat tgaatttcaa aataaggaaa cagagttatg gcactttggg gcgggcgttt
    31261 tacccaggca gcagatcaac ggttcaaaca attcaacgac tcactgcgct ttgattaccg
    31321 tctggcggag caggatattg ttggctctgt ggcctggtcc aaagccctgg tcacggtagg
    31381 cgtgttaacc gcagaagagc aggcgcaact ggaagaggcg ctgagcgtgc tgctggaaga
    31441 tgttcgcgcc aggccacaac aaatccttga aagcgacgcc gaagatatcc atagctgggt
    31501 ggaaggcaaa ctgatcgaca aagtgggcca gttaggcaaa aagctgcata ccgggcgtag
    31561 tcgtaacgat caggtagcga ctgacctgaa actgtggtgc aaagataccg ttagcgagtt
    31621 actgacggct aaccgacagc tgcaatcggc gctggtggaa accgcacaaa acaatcagga
    31681 cgcggtcatg ccaggttaca ctcacctgca acgcgcccag ccggtgacgt tcgcgcactg
    31741 gtgcctggcc tatgttgaga tgctggcgcg tgatgaaagc cgtttgcagg atgcgcttaa
    31801 gcgtctggat gtcagcccat taggctgtgg cgcgctggcg ggaacggcct atgaaatcga
    31861 ccgtgaacag ttagcaggct ggctgggctt tgcttcggcg acccgtaaca gtctggacag
    31921 cgtttctgac cgtgaccatg tgttggaact gctgtcggct gccgctatcg gcatggtgca
    31981 tctgtcgcgt tttgctgaag atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga
    32041 gctttctgac cgcgtgactt ccggttcatc attaatgccg cagaagaaaa acccggatgc
    32101 gctggagctg attcgcggta aatgcggtcg ggtgcagggt gcgttaaccg gcatgatgat
    32161 gacgctgaaa ggtttgccgc tggcttacaa caaagatatg caggaagaca aagaaggtct
    32221 gttcgacgcg ctcgatacct ggctggactg cctgcatatg gcggcgctgg tgctggacgg
    32281 cattcaggtg aaacgtccac gttgccagga agcggctcag cagggttacg ccaacgccac
    32341 cgaactggcg gattacctgg tggcgaaagg cgtacctttc cgcgaggcgc accatatcgt
    32401 gggtgaagcg gtggtggaag ccattcgtca gggcaaaccg ctggaagatc tgccgctcga
    32461 cgagttgcag aaattcagcc cggtgattgg cgaagatgtc tatccgattt tgtcgctgca
    32521 atcgtgcctc gacaagcgtg cggcaaaagg cggcgtctca ccgcagcagg tggcccaagc
    32581 gattgctttt gcgcaggcgc ggttggggta agaacattta tatgtacaaa tttgagcctg
    32641 gcttatcgcc gggctttttt atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc
    32701 acgttggctt tagttattcg agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt
    32761 aaaagaggtg ccgctccgtt tctgtgagca attatcagtc agaatgcttg atagggataa
    32821 tcgttcattg ctattctacc tatcgccatg aactatcgtg gcgatggagg atggataatg
    32881 aatattcgtg atcttgagta cctggtggca ttggctgaac accgccattt tcggcgtgcg
    32941 gcagattcct gccacgttag ccagccgacg cttagcgggc aaattcgtaa gctggaagat
    33001 gagctgggcg tgatgttgct ggagcggacc agccgtaaag tgttgttcac ccaggcggga
    33061 atgctgctgg tggatcaggc gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg
    33121 gcaagccagc agggcgagac gatgtccgga ccgctgcata ttggtttgat tcccacggtt
    33181 ggaccgtacc tgctaccgca tattatcccg atgctgcacc agacctttcc aaagctggaa
    33241 atgtatctgc atgaagcaca gacccaccag ttactggcgc aactggacag tggcaaactc
    33301 gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt
    33361 gatgagccaa tgttgctggc tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta
    33421 ccgatggccg atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc
    33481 gatcaggcaa tgggtttctg ttttgaagcc ggggcggatg aagatacaca cttccgcgcg
    33541 accagcctgg agacgctgcg caacatggtg gcggcaggta gcgggatcac tttactgcca
    33601 gcgctggctg tgccgccgga gcgcaaacgc gatggggttg tttatctgcc gtgcattaag
    33661 ccggaaccac gccgcactat tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc
    33721 tatgagcagc tggcagaggc catccgagca agaatggatg gctatttcga taaagtgtta
    33781 aaacaggcgg tttaaaccgt ttaacgcagc tacccgatag gcttccgcca tggtcgggta
    33841 gttaaaggtg gtgttgacga agtactcaat agtgttgccg ccacctttct gttccataat
    33901 cgcctgaccg atatgaataa tttcggcagc gcgctcgcca aagcagtgaa tacccagaat
    33961 ctcttttgtt tcccgatgga acaaaatttt cagcgtgccc acgttcatgc cgacgatttg
    34021 tgcgcgtgcc agatgtttaa actgggcgcg gcccacttca tatggcactt tcatcgcggt
    34081 cagctgctgt tcggttttgc ccacagagct gatttccggg atggtgtaaa tgccggtagg
    34141 gatatcttca atcagatgcg cggtggcttc gccttttacc agcgcctgcg cggcaatgcg
    34201 cccctggtca taggccgctg atgccaggct cggataacca atcacgtcgc ccaccgcgta
    34261 aacgtgcggc tgtgcggtct gatacatgct gttgaccttc agctgtccgc gactgtcagt
    34321 ttccagccca atgttctgta acgccagcga atcggtatta ccggtacgac cgttggcata
    34381 gagcaggcag tcagctttca gttttttacc cgacttcaga tgcatgatca caccgtcgtc
    34441 acagccttcg atcttctcgt actcttcgtt gtgacgaatc actacgccac tgttccagaa
    34501 gtgataggag agagaatctg acatctcttg atcgagaaat gccagcaggc gatcgcgggt
    34561 gttgatcaga tccactttta catccatacc gcggaagatc gacgcatatt cacagccgat
    34621 cactccagca ccatagataa gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc
    34681 gctgtcgtaa atgcgtggat gggtgaaatc aacatctgtt ggatgatatg gacgagagcc
    34741 gcaggcaata acaaattttt cagcggttag tgtttcgacg ctgccgtccg ggcaatccag
    34801 cgccaacgta tgctcgtcaa caaagcgagc gttcccctgc aatatttcac agtgattacg
    34861 ttcgtaaaat ccctgacgca tgcgcgtttg ttgattaatc acgttatcgg catggttaag
    34921 gatatcggca aaagaagagc ggagcagtcg cgaatggtcg ctgtatagtg ggttttgatt
    34981 gaattctata atgcggctga cggcgtgacg gagagctttc gacgggatgg tgccccagtg
    35041 ggtgcaaccg ccgccaacat tttgataacg ctcgataact gcgacgcgca caccttgctt
    35101 aaccaggccc attgcagcgc cttcgccgcc ggggccggaa cctattacta tggcatcgta
    35161 atcgtaggaa tgtggcatgg tagggcttac ctgttcttat acataaaagc aacagaatgg
    35221 taacattcta tcgcgggtaa gccaattgat ccccgtcatt tatctggcta tatcctgagc
    35281 ggcctttgct ttgtctgttt cttacttttg ccctgacgtt ttattggatt tttatcgacg
    35341 atactctccg tttaagcggc aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg
    35401 ttttattgcg ttaccgttca ttcacaatac tggagcaatc cagtatgttc attctctggt
    35461 atagtgccag cagtactttt ggcaaggatt cagacatcgt gatgggcata agagcgcaac
    35521 aaaaagaaaa aacccgccgt tcgctggtgg aagccgcatt tagccaatta agtgctgaac
    35581 gcagcttcgc cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct
    35641 ctttttatcg gcatttccgc gacgtagacg aactgggtct gaccatggtt gatgagagcg
    35701 gtttaatgct acgccaactc atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg
    35761 tgatccgcac ctcggtctcc acatttatgg agttcatcgg taataatcct aacgccttcc
    35821 ggttattatt gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg
    35881 aaattcagca cttcattgcg gaacttgcgg actatctgga actcgaaaac catatgccgc
    35941 gtgcgtttac tgaagcgcaa gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg
    36001 aggcgttgga cgtcggcgtc gaacaacgtc ggcaattaga agagcgactg gtactgcaac
    36061 tgcgaatgat ttcgaaaggg gcttattact ggtatcgccg tgaacaagag aaaaccgcaa
    36121 ttattccggg aaatgtgaag gacgagtaat gaaacaagca aatcaagata gaggtacgct
    36181 gctgctggcg ttagttgctg gcttatcgat taatggtact ttcgcagcgc tgtttagctc
    36241 cattgtgcca ttttctgtat tcccgattat ttccctggtg ctgacggttt actgcctgca
    36301 tcaacgttat cttaatcgca ctatgccggt aggcttgccg ggtctggcag ctgcctgttt
    36361 tattctcggc gtactgctgt acagcacggt agttcgtgcg gaatatccgg atatcggctc
    36421 taacttcttc ccggcagtac tctccgtcat tatggtgttc tggattggcg cgaagatgcg
    36481 taaccgtaag caggaagttg ctgagtaatc ggtattatgc cggatgctgg cgcatccggc
    36541 atgggtttta cttcgcggtc agtaatacgc cgcactccat atggtgcgtg taggggaact
    36601 gatcaaacag agccagacgt tcgaccttgt gcgtctggct taatgtttcc agattcttgc
    36661 ataacgtttc cgggttacag gagatgtaca aaatacacgg atacgcctgc accattttct
    36721 cggtttcact gtccagaccg ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac
    36781 tctttaagtc gatcccttgc aggcggttaa actcgcgcac accattcatc gcctgagtaa
    36841 attcttctgc cgccatacga ataatttgta cgttatcaat atggttagct gcgatgttgt
    36901 attgcgcagc agcaaccgac ggcttagcga tttcggtggc taatacccga tcaaaattgc
    36961 gcgccagcgc taatgaaaag ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg
    37021 agcctttggt tacgtccagc gcccattcca gcatctgaat attcatcgcc gcgttcggct
    37081 gggtaaagct gttttctacc tgacggtaga tcatctcttt ccctgcgacc ggcagacgct
    37141 cgtcgatgta atcttgatcc agctcgattt tggttttcgt tgcccgacca atcagatgca
    37201 cattcagatt ctgcgcgcgc agtgcatcgc gcagggcctc cgcttcctga cgccactcat
    37261 catccagctt cttatggtat agcagggaaa ccaccgcctg attactcagc gtggtgaggt
    37321 aatcaatctg gaacaacttg tggcgcagaa cgggattatt acgcacaccc gcaatcatcg
    37381 ccgtcatcaa ctggttgata agttcactgg cggcggggaa gctatccacg cggatacggc
    37441 ttttggtttg ttgatcgaaa atgatgtgat acaggtcatc gccatcgtgc cagatgcgga
    37501 actccgcacg catccggtaa tgactgaccg gcgagcgaaa cacttccgga accaggtcag
    37561 aaaacggtgc catcatactt tgcaaacgta ccactttttc ggctaactgc gcttcatact
    37621 gttctgttgg aaggtgttcg ggggtcatga tgtatcctga aaaattaaag tacgcggcga
    37681 ttgtagggat tgctcatcag atgtccagat cttgatgaat tcctatttgt gagctacgtc
    37741 tggacagtaa cttgttacaa cctgtagcat ccacttgtcg gtcctgtgag ttaataggga
    37801 atccagtgcg aatctggagc tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat
    37861 gcggacactg ccatccggtg ggaagtcatc atctcttagt atcttagata cccctccaag
    37921 cccgaagacc tgccggccaa cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa
    37981 cctgcggcat ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg
    38041 acggcgtgtt ctgtcacagc cttttccgct tgggcacagg ataccagccc ggatactctc
    38101 gtcgttactg ctaaccgttt tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt
    38161 gtgacccgtc aggatatcga ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt
    38221 cttccgggcg tcgatatcac ccaaaacggc ggttcaggtc agctctcatc tatttttatt
    38281 cgcggtacaa atgccagtca tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg
    38341 ggggtgagtg gttctgccga ccttagccag ttccctattg cgcttgtcca gcgtgttgaa
    38401 tatatccgtg ggccgcgctc cgctgtttat ggttccgatg caataggcgg ggtggtgaat
    38461 atcatcacga cgcgcgatga acccggaacg gaaatttcag cagggtgggg aagcaatagt
    38521 tatcagaact atgatgtctc tacgcagcaa caactggggg ataagacacg gggaacgctg
    38581 ttgggcgatt atgcccatac tcatggttat gatgttgttg cctatggtaa tacaggaacg
    38641 caagcgcaga cagataacga tggtttttta agtaaaacgc tttatggcgc gctggagcat
    38701 aactttactg atgcctggag cggctttgtg cgcggctatg gctatgataa ccgtaccaat
    38761 tatgacgcgt attattctcc cggttcaccg ttgctcgata cccgtaaact ctatagccaa
    38821 agttgggacg ccgggctgcg ctataacggc gaactgatta aatcacaact cattaccagc
    38881 tatagccata gcaaagatta caactacgat ccccattatg gtcgttatga ttcgtcggcg
    38941 acgctcgatg agatgaagca atacaccgtc cagtgggcaa acaatgtcat cgttggtcac
    39001 ggtagtattg gtgcgggtgt cgactggcag aaacagacta cgacgccggg tacaggttat
    39061 gttgaggatg gatatgatca acgtaatacc ggcatctatc tgaccgggct gcaacaagtc
    39121 ggcgatttta cctttgaagg cgcagcacgc agtgacgata actcacagtt tggtcgtcat
    39181 ggaacctggc aaaccagcgc cggttgggaa ttcatcgaag gttatcgctt cattgcttcc
    39241 tacgggacat cttataaggc accaaatctg gggcaactgt atggcttcta cggaaatccg
    39301 aatctggacc cggagaaaag caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg
    39361 gtgaactggc gtatttccgg atatcgtaac gatgtcagtg acttgatcga ttatgatgat
    39421 cacaccctga aatattacaa cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc
    39481 aattttgata ccggaccact gacgcatact gtgagttatg attatgtcga tgcgcgcaat
    39541 gcgattaccg acacgccgtt gttacgccgt gctaaacagc aggtgaaata ccagctcgac
    39601 tggcagttgt atgacttcga ctggggtatt acttatcagt atttaggcac tcgctatgat
    39661 aaggattact catcttatcc ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat
    39721 cttgcggttg cgtatccggt cacctctcac ctgacagttc gtggtaaaat agccaacctg
    39781 ttcgacaaag attatgagac agtctatggc taccaaactg caggacggga atacaccttg
    39841 tctggcagct acaccttctg aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg
    39901 tgggttgtcg gtctatgacg agatccggca tctcttaccg gatctccatt acatttatgc
    39961 tttcgataac gtcgctttcc cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt
    40021 ggtggcaatt gtcaccgcgg tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg
    40081 caacactgcc agtaccgttt cacttcctgc attacgcgaa aagttcgact tcccggttgt
    40141 tggtgtcgtg ccggcgatta aacctgctgc acgtctgacg gcaaatggca ttgtcggatt
    40201 actggcaacc cgcggaacag ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc
    40261 taatgaatgc cagatagaaa tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa
    40321 gctacatggc gaagatgttt ctctggatgc actaaaacgt atcctacgcc cgtggttaag
    40381 aatgaaagag ccgccagata ccgttgtatt gggttgcacc catttccctc tactacaaga
    40441 agaactgtta caagtgctgc cagagggaac ccggctggtg gattctggcg cagcgattgc
    40501 tcgccgaacg gcctggttgt tagaacatga agccccggat gcaaaatctg ccgatgcgaa
    40561 tattgccttt tgtatggcaa tgacgccaga agctgaacaa ttattgcccg ttttacagcg
    40621 ttacggcttc gaaacgctcg aaaaactggc agttttaggc tgatttggtt gaatgttgcg
    40681 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    40741 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
    40801 ctcaacagag aaaagcaaaa aaatgc
//
LOCUS       DAJTXQ010000019        40665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000019 DAJTXQ010000000
VERSION     DAJTXQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.019"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            209..1306
                     /gene="mltA"
                     /locus_tag="OO515_000998"
     CDS             209..1306
                     /gene="mltA"
                     /locus_tag="OO515_000998"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6574990.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1383..2189
                     /gene="tcdA"
                     /locus_tag="OO515_000999"
     CDS             1383..2189
                     /gene="tcdA"
                     /locus_tag="OO515_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6574991.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OO515_001000"
     CDS             complement(2240..2683)
                     /gene="csdE"
                     /locus_tag="OO515_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6574992.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OO515_001001"
     CDS             complement(2683..3888)
                     /gene="csdA"
                     /locus_tag="OO515_001001"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6574993.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4080..4307
                     /locus_tag="OO515_001002"
     CDS             4080..4307
                     /locus_tag="OO515_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6574994.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4658..5575
                     /gene="gcvA"
                     /locus_tag="OO515_001003"
     CDS             4658..5575
                     /gene="gcvA"
                     /locus_tag="OO515_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6574995.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5594..5989
                     /locus_tag="OO515_001004"
     CDS             5594..5989
                     /locus_tag="OO515_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6574996.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5982..7082
                     /gene="rlmM"
                     /locus_tag="OO515_001005"
     CDS             5982..7082
                     /gene="rlmM"
                     /locus_tag="OO515_001005"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6574997.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OO515_001006"
     CDS             complement(7126..7857)
                     /gene="fucR"
                     /locus_tag="OO515_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6574998.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OO515_001007"
     CDS             complement(7915..8337)
                     /gene="fucU"
                     /locus_tag="OO515_001007"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6574999.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OO515_001008"
     CDS             complement(8339..9757)
                     /gene="fucK"
                     /locus_tag="OO515_001008"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6575000.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OO515_001009"
                     /pseudo
     CDS             complement(9866..11641)
                     /gene="fucI"
                     /locus_tag="OO515_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OO515_001010"
     CDS             complement(11674..12990)
                     /gene="fucP"
                     /locus_tag="OO515_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6575001.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13537..14184
                     /gene="fucA"
                     /locus_tag="OO515_001011"
     CDS             13537..14184
                     /gene="fucA"
                     /locus_tag="OO515_001011"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6575002.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14212..15360
                     /gene="fucO"
                     /locus_tag="OO515_001012"
     CDS             14212..15360
                     /gene="fucO"
                     /locus_tag="OO515_001012"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6575003.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OO515_001013"
     CDS             complement(15465..16220)
                     /gene="xni"
                     /locus_tag="OO515_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6575004.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OO515_001014"
     CDS             complement(16332..17699)
                     /gene="sdaB"
                     /locus_tag="OO515_001014"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6575005.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OO515_001015"
     CDS             complement(17757..19046)
                     /gene="sdaC"
                     /locus_tag="OO515_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6575006.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OO515_001016"
                     /gene_synonym="ygdH"
     CDS             complement(19602..20966)
                     /gene="ppnN"
                     /locus_tag="OO515_001016"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6575007.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OO515_001017"
     CDS             complement(21078..21926)
                     /gene="queF"
                     /locus_tag="OO515_001017"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6575008.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21994..22539
                     /gene="syd"
                     /locus_tag="OO515_001018"
     CDS             21994..22539
                     /gene="syd"
                     /locus_tag="OO515_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6575009.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23161..23490
                     /locus_tag="OO515_001019"
     CDS             23161..23490
                     /locus_tag="OO515_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6575010.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23490..24272
                     /gene="truC"
                     /locus_tag="OO515_001020"
     CDS             23490..24272
                     /gene="truC"
                     /locus_tag="OO515_001020"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6575011.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24290..24739
                     /locus_tag="OO515_001021"
     CDS             24290..24739
                     /locus_tag="OO515_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6575012.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25174..26526
                     /gene="gudP"
                     /locus_tag="OO515_001022"
     CDS             25174..26526
                     /gene="gudP"
                     /locus_tag="OO515_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6575013.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26528..27868
                     /locus_tag="OO515_001023"
     CDS             26528..27868
                     /locus_tag="OO515_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6575014.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27889..29229
                     /gene="gudD"
                     /locus_tag="OO515_001024"
     CDS             27889..29229
                     /gene="gudD"
                     /locus_tag="OO515_001024"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6575015.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OO515_001025"
     CDS             complement(29461..32217)
                     /gene="barA"
                     /locus_tag="OO515_001025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6575016.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32274..33575
                     /gene="rlmD"
                     /locus_tag="OO515_001026"
     CDS             32274..33575
                     /gene="rlmD"
                     /locus_tag="OO515_001026"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6575017.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33623..35857
                     /locus_tag="OO515_001027"
     CDS             33623..35857
                     /locus_tag="OO515_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6575018.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35935..36183
                     /gene="mazE"
                     /locus_tag="OO515_001028"
     CDS             35935..36183
                     /gene="mazE"
                     /locus_tag="OO515_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6575019.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36183..36518
                     /gene="mazF"
                     /locus_tag="OO515_001029"
     CDS             36183..36518
                     /gene="mazF"
                     /locus_tag="OO515_001029"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6575020.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36589..37380
                     /gene="mazG"
                     /locus_tag="OO515_001030"
     CDS             36589..37380
                     /gene="mazG"
                     /locus_tag="OO515_001030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6575021.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37608..39245
                     /gene="pyrG"
                     /locus_tag="OO515_001031"
     CDS             37608..39245
                     /gene="pyrG"
                     /locus_tag="OO515_001031"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6575022.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39333..40631
                     /gene="eno"
                     /locus_tag="OO515_001032"
     CDS             39333..40631
                     /gene="eno"
                     /locus_tag="OO515_001032"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6575023.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10072 a         9840 c        10816 g         9937 t
ORIGIN      
        1 tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt taccccgatc
       61 acggatctgg catgattttg aacaaaaaca aatcactaaa gcctattttt tgtgcaaaat
      121 ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc ttcgttgcgc
      181 cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt accttcttat
      241 gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca
      301 atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag atgccgttgg
      361 cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta attcgtcacc
      421 gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc tgcgcgcagg
      481 cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg aaggtgccga
      541 caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg cgcgccatac
      601 ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg gtcgtctgcc
      661 gtctcgtgcg gagatctacg cgggggcatt gagtgataaa tatattctcg cttacagtaa
      721 ctccctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg actttggtga
      781 tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct atcgcagcat
      841 tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat
      901 tcgtcactgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg aacagaaccc
      961 gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc
     1021 gctggttggt cgcgcgtcag ttgcctctga tcgttccatt attccgccag gtactacctt
     1081 gctggcagaa gtgccgttgc tggataataa cggcaaattt aatggtcagt acgaactgcg
     1141 tctgatggtg gcgctggatg tcggtggtgc aatcaaaggc caacacttcg atatctatca
     1201 agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg gacgtgtctg
     1261 ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc ggctgatgtg ttattctgta
     1321 taccgttttt acggatacga cagggtgagg gataacctca ccctttttaa ttctgaggtg
     1381 ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt
     1441 atggtgaaaa agcgttgcaa ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg
     1501 gtgtcggttc ctgggcggcg gaagcgctgg cgcgcacggg gattggcgca atcacgctta
     1561 tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca aattcacgct ctgcgcgata
     1621 acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat tcgccagatt aacccggagt
     1681 gccgtgtaac ggtggtggat gatttcgtga cgccggataa cgtagcgcag tatatgagcg
     1741 tgggttattc gtacgtgatt gatgccattg atagtgtacg gcccaaagcg gcgctgattg
     1801 cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg tggcgcgggt gggcagattg
     1861 acccgacgca gattcaggtt accgatctgg cgaaaacgat tcaggatccg ctggcggcga
     1921 agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg
     1981 gcgtcgattg cgtgttttct actgaagcgc tggtgtaccc gcagtcagac ggtactgtgt
     2041 gtgcgatgaa agccacggca gaagggccga agcggatgga ttgtgcatct ggatttggcg
     2101 cggcaacgat ggtgaccgcc acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga
     2161 tgatggcgaa agcggcgcgt cagggttaaa caatcaggtc ggatgagacg cgaacgtctt
     2221 atccgacccg gttctatgct taaacctgct tcgtcgcagc gataatcgcc tcgcttaacg
     2281 catttaaccc ctggctgcgt gaggcgctaa gttgcgcacg caatcccagc tcatcaaaca
     2341 atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt cccctcaacg gcagtcaaca
     2401 gcaccgccag caggccgcgc acaatgcgcc cttcgctatc gccaaagaag tgcattttac
     2461 cgttttcagt cacggtatat cccagccaga cgcggttttc gcatccggca atctctttag
     2521 cctgcgcttt taactcgtct ggcaaagccg gaagctgttt ccccagcatg atcaactggc
     2581 gatatttatc ttcccattgc gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta
     2641 cggttgtgcc gaacggatgt ccggcgaatt gcgggttcgt cattaatcca ccaataattc
     2701 cagcgcgcgg tcaacggcat tcaccagcgc atccacatca ctctttgtat tatatggcgc
     2761 aaaagaggcg cgcagtgtgc cggttacgcc taattctgcc agtagcggct gagcgcaatg
     2821 ttgcccggcc cgcagggcaa taccgtactc cgccagcagc gtcaccatat cgctgtgatg
     2881 aacgccagca aaatcaaagg ccagcaggct ggaatcctgg cagcggaatg agcggaagcc
     2941 aggacgtttt gccagcgcat cttccgccag cgtcgccagg ctgcgactcc aactttctgc
     3001 ctggttgata tcgtaatctg ccagccattc cagcgccgcg cttaatccta tgacaccagc
     3061 cacatttggc gttcctgctt ccagtttcca cggcgcagat tgagtcgtga agccgtcaaa
     3121 actcacttcg tgaatcattt tgccgccacc cagccagggc gacatcgctt ccagcagttc
     3181 tgatttgcca tacagcacgc cgatacccgt cgggccatac agtttgtgac cagagaaagc
     3241 atagaaatcg atatctagtt gctgaacatc tgcggggaaa tgcaccgccc cctgagcacc
     3301 atcaaccatc accaccatcc cggctgaatg agcaaaggta atcgctcgcg ccagatccgg
     3361 gcaaccgcca gtaacgttcg acatctgacc caacgccaga atccgactac ggggagtaat
     3421 cagttctggc aacaaatcga catccggcag tcgctgcgca ttaagcggca atttcaccac
     3481 tttggctcca gtttgttggg cgaccatcag ccaggggacg aggttagcgt ggtgttctgc
     3541 cacgctgaca ataatctcat cgcccggttg cagacgcgga cgcgcatagc attgtgccac
     3601 catgttgatg gattcagtgg tgccgcgcgt ccagacgata gttttatcat ccggtgcatt
     3661 cagtaattgc gccactttct ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc
     3721 ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg taaaactgtc gagtggcttc
     3781 aaccacggct tcaggtttaa gcgcggtcgc ggcgctatcg agatagacgc ccgcatcctg
     3841 tagtgcggga aactgggcgc gaaactgcgc gggattaaaa acgttcatgg tactcctcgg
     3901 cttgatgcac cgatcgtgac gcaaaattca ctttgataca aggagagtgc taaccatcag
     3961 aattatctgg atatcctggc gaagccgcgt aagtaacata tgctgaatag tgttaggcgt
     4021 taattttagc ctgttaatca atactattaa gatttattaa tcgctttaag gagaagatga
     4081 tgaaaaagac tgccgcaatt atttctgcct gtatgctgac ttttgccctg agcgcctgtt
     4141 ccggttcgaa ctatgtgatg cacaccaatg acggacgtac catcgtctct gacggcaaac
     4201 cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc aacaaacagc
     4261 agatcaaccg tactgacgtg aaagagatgg tcgaactgga tcagtaattc gcgatcgcaa
     4321 ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac agacagggta
     4381 aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc agaccaattg
     4441 caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac acgcattccg
     4501 ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc tggtagaagc
     4561 tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac agtgtgatct
     4621 aattgttaaa ttaatttaac atcaaagttt aaaagccatg tctaaacgat taccaccgct
     4681 aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc gcgcagcaga
     4741 agagcttttc gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg aggatttttt
     4801 ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag
     4861 ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc gtaaactcca
     4921 ggcccgtagc gccaaggggg cgttgacggt cagtttactc cccagtttcg ccattcattg
     4981 gttggttccg cgactttcca gctttaattc agcttatccg ggaattgacg ttcgaatcca
     5041 ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga tattttatgg
     5101 tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt tattgccggt
     5161 gtgttcgccg ctactgctga caggcgaaaa acccttgaag accccggaag atctggctaa
     5221 acatacgtta ttacatgatg cgtcacgccg tgactggcag acatataccc gacagttggg
     5281 gttaaatcat atcaacgttc agcaagggcc aatttttagt catagcgcca tggtgctgca
     5341 agcggctatt cacgggcagg gagtggcgct ggcaaataac gtgatggcgc aatctgaaat
     5401 cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa acgcttttta
     5461 tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc gccaatggat
     5521 cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac aataatttac
     5581 gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta gcggcttcat
     5641 ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg gggccgttga
     5701 gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg cgatcttagg
     5761 tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta gcgttttcct
     5821 cgcgttaggc acggtgttgt tcagcggcag cctttattgc ctggcgctgt cccatctgcg
     5881 tttgtgggca tttgtcacac cggttggcgg cgtgagcttc ctcgcgggct gggcgttaat
     5941 gttagttggt gctatccgtt taaagcgcaa gggcgtaagt catgaataag gttgtattgc
     6001 tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa gccggccagc
     6061 gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt
     6121 atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca ttaatttttg
     6181 cccgccagtg gtttgtggtg ggggaactct tgcagcattt gccgccagaa gatcgtatta
     6241 cccccattgt cgacatgttg cagggcgtag tagagaaggg cggtgaactg cgtgttgaag
     6301 ttgccgatac caacgaaagc aaagagttgc tgaaattctg ccgtaaattc accgtgccgc
     6361 tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta tgaaacgccg aagcgtccgg
     6421 ttgtgcatgt attctttatt gcaccaggct gctgctatac cggttactca tacagcaaca
     6481 ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagag gcgccgagtc
     6541 gttccacgct caaactggaa gaggcatttc atgtgtttat tcctgcggat gagtgggatg
     6601 aacgcctggc gaacgggatg tgggcggtgg atttaggcgc ttgccctggc ggctggacct
     6661 accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg atggcgcaaa
     6721 gtctgatgga taccgggcag gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga
     6781 cacgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggct aaagttgcgg
     6841 cattgatggc gcagtggctg gttaatggct ggtgccgtga aaccattttc aatctcaaac
     6901 tgccgatgaa aaaacgctac gaagaagtat cacacaatct ggcgtatatt caggcacagc
     6961 ttgatgaaca tggcataaat gctcagattc aggcgcggca gttgtatcac gatcgcgaag
     7021 aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat
     7081 aacaggcaag gccggggcgt cgtcttcggc ctgctaccct ttccgtcagg ctgttaccaa
     7141 agaagttgca acctggcgct catcagaaat aatgtgcgtt acctcatcca gatgcccgat
     7201 gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg attgcgcggc
     7261 acgttttaat agcatcgatt tgtaatcagc gttgatcgca ttggagtccc acagtgcgcc
     7321 gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg atttaagttg
     7381 ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact
     7441 gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc tattggtgaa
     7501 gacctgaatg ttgatgtcag gcaactggcg tgccagatac cagcaagttg aactggcgtc
     7561 taaggctatc accatccctt cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt
     7621 atgcgcataa tggcttttca gcctgatgtg aaagggatcg ccactgtctt gattttgacg
     7681 gtggatatat ttagcgcgtc catgattgcg cagaatttta ccctgcgtct gtaattcatt
     7741 gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag cttccgtggt
     7801 caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt
     7861 tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccgatat gagattacgg
     7921 tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg tgataacgat
     7981 cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg
     8041 acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag gatcgagagt
     8101 gtcaccttct accgctgcca tcatcaccag cggcggggca taactgtcca gctcaaataa
     8161 tgggataatc gcttggagca agtcgctcac caacaggcca tcagcgcgga tcacctgcgg
     8221 tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc catgtcccat
     8281 ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt tcagcatttc
     8341 acacttcctc tataaattca ggttcagttt gcgggtagaa ataacggtac tgataatgaa
     8401 tctgtgcacg ggcttcttcc gggctgttaa attcccctac gccataccaa ccgaacagcg
     8461 cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt taccggaata tcaagcatat
     8521 tggctttaat ctgattccac aatgtgttgc gacttcctcc accgactaac aataattcag
     8581 aggctttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat tgcgcagtta
     8641 acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtc actccttgcc
     8701 agccagcgtt ctgacacgac aataaatcac actgcatttt cacaccatcc gcgccaggtg
     8761 cgatcagacg agcttcttca atcaacattt gccagggtgt ttcagccgtc cagaacagtt
     8821 ttctcaccca ttccagcacg ccggatgcca gccattgcat acctgggtta tacaaccctg
     8881 cctggctatc cagttcgcag gtagaatcgg cgtactgact taacagcgaa gtatcaacct
     8941 gggcgctacg gaccattaaa atttcccatg taccggaaga gagcacgggt tcattttggt
     9001 cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc accggtatgc
     9061 caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc tgttcacccg
     9121 cttccaccag acgagggaag agtcggcgtg gaataccggt ggcttgtaaa atttgcggac
     9181 tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgtg atatcagtag
     9241 tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag gcgtgcgcgc
     9301 gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac gtattgaaac
     9361 taaaggctcc gacgccagaa atagcctgca accgctgtgc ggagatcaac tgttcaatat
     9421 tgtccataac cgctgctgtt cgcggacatt tccagctaat gatcggatag agcagattgc
     9481 cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata ccgcggatgt
     9541 ggcattcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg
     9601 cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg gcattaggcg
     9661 ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg acattggtcg
     9721 cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca atatagccgg
     9781 ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc cggagcgacc
     9841 gggcatcaca tcagggagta atgtattaac gcttgtacaa cggaccgtag ttctggcaag
     9901 cgcggtaatc ctggccttca atatccatgc cgtgcgcagc ccaggcagaa ggacgataca
     9961 ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag gcgagagtga
    10021 taaagtctgc gccaacgtgg ccgatggtca gaaccccatg gttagcgccc cagttcgcca
    10081 ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca aaccaggtgg
    10141 ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc ggcaattcca
    10201 cgctccagcc ttccgcgatt tgcagtaccg atcccaggcc tttgatgatg ttgacacgag
    10261 tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg ccgccacgga
    10321 agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca gcctcttgct
    10381 gagagatttc ccagtgtggc ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg
    10441 aaccgtccag cgcagcagaa cctgagttga tcaaatggat gatgccgtgt tctgccagtc
    10501 catcgagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag gtacgcacat
    10561 cggcaaatac ctgagcggtg ccggtgagct ggtgacccat tagcattgcc acgccgttaa
    10621 gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac gccattccag tcaaatgaac
    10681 tgttgaggat cgcttcggcg gtgtcaccat tgggatattg atcggtccag tgacgttgcc
    10741 cctggaagcc cgcagcgatg gcgttgtagc caagtgattc ttccacgcga ccaatatcgg
    10801 ccagtttgct gttgccttgc atcatgtcgc ggatacacat cgccatcagt aaactttcgc
    10861 gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt tcatcttcgc
    10921 catagcggaa gtttttatca gcccaggtca gtgccatttc cagttcggct tcgtcgtaaa
    10981 tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact ttcattccca
    11041 gccaggattc aaagaagttg tgatcaacaa tagaaccggc gatacccatc gaaacgccgc
    11101 ccagcgacag atagctttta cctttcatgc tggcgacggc caaaccggcg cgggcaaagc
    11161 gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat
    11221 gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga gccgctgcca
    11281 ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc gggcgggttg
    11341 gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta atggtgaggc
    11401 ctacattcta gctgctgaat ttttcttcgc aagcagcggc ttcagccata cccgcgatac
    11461 aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt ttctcggtca
    11521 gcagtgcggc cgtagctttc gccatattca ttgtttgttc ttcaagcgac tcacgaacac
    11581 ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg atttttttca
    11641 ttacagattc ctcactttat tcggaaaata tgttcagtta gttgccgttt gagagcggaa
    11701 acgggcaaag ataaagatga ccgcgaagca gagtgtgggg atcagttcag cagtggggat
    11761 gttgcccgcc gcgtcactga caaaacccat gaccggagtg acaataccgc cgccaataat
    11821 ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct taatgcccag
    11881 cgagaagatt gttgggtact gaatcgacat aaaggcgctg cataaagtca gggctattaa
    11941 gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga ttaatgcgta
    12001 ggcggccagg actttgtgtg gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc
    12061 aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg cagtcatacc
    12121 tggaatttct tctacagcgt agcgaatcaa atagctccag caggccgttt gtgcgccaac
    12181 gtagcagaat tgcgctaata ctgcccagcg ccagtggcga atacgcgcta ggcgagaaag
    12241 cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca atgccgggaa
    12301 tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga tcatataagg
    12361 tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg gagacatttt
    12421 atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagtcttt gcccaaagac
    12481 aaccgcgata attgcgccaa acgagttaaa tgtttgcgca agatttaagc ggaagtgacc
    12541 actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa
    12601 tccggctgca ataataaata ggccaactaa aaataaggtg tagttcatta tttctgcagc
    12661 gggccagaat aatgcagcgc ccagggcata taaaaataac ccagtaataa ttcctgcttt
    12721 ataactgagt tttttcatca atatcccagc aggaattggg ataatgaaat aaccaaagta
    12781 aaaggccgat tggatcaggc cagcctggaa atttgtcagc gtaaaagcct gctggaattg
    12841 aggtaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca gtgagcacag
    12901 cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta ccgcacggta
    12961 actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga agcttaagca
    13021 cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca tatcagtgaa
    13081 tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg ctctgtaata
    13141 tgacggcggt cacacttatt agctggaagg tgactttatg tgactaccat cactttaata
    13201 atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc agatgactgt
    13261 attaattgtt tatgccgaaa acgggcattc ctggcttggg tgaatagaaa aatgaccgtt
    13321 ttgataaata tttttaagag cggtcatttt taatggggtt ataaaaagag aacttgcagt
    13381 tatttactgt gattagttga gctaggtcac aaaatataag cttgtgaatt tctttcttgt
    13441 taatcaggaa gaagatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac
    13501 attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt
    13561 cagattattg acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg
    13621 aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa
    13681 aaactgacgg agtcgcatat tgtctttatt gatggcaacg gtaaacatga ggaaggaaag
    13741 ctcccctcaa gcgaatggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac
    13801 gcggttgttc acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc
    13861 cccgctattc actacatgat tgcggcggca ggtggtaatt ctattccttg cgcgccttat
    13921 gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag
    13981 gcaactttgt tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta
    14041 tggctggcgc atgaagttga agtgctggcg caactttacg tggcgaccct ggcgattacg
    14101 gacccggtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc
    14161 tatgggttac ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac
    14221 agaatgattc tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat
    14281 gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa
    14341 tgcggcgtgg tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt
    14401 tacgacggcg tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc
    14461 cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt
    14521 aaagcgattg gcattatcag caacaatccg gagtttgccg atgtgcgtag cctggaaggg
    14581 ctttccccga ccaataaacc cagtgtaccg attctggcaa ttcctaccac agcaggtact
    14641 gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg caagtttgtt
    14701 tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt
    14761 atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg
    14821 tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc
    14881 attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg
    14941 ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt
    15001 atggcgcatc cactgggcgc gttttacaac actccacacg gtgttgcgaa cgccatcctg
    15061 ttaccgcatg tcatgcacta taacgctgac tttaccggtg agaagtaccg cgatatcgcg
    15121 cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt
    15181 gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt
    15241 gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt ttgtaccggt
    15301 ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac cgcctggtaa
    15361 atgcgctgat gtgataatgc cggatacgac gctagccgcg tcttatccgg tatataagtg
    15421 cattaaattc aatgtgttga taatcgtttg catccggctc gccgttaccg taccaaccgc
    15481 aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac
    15541 agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc
    15601 agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt ggcgcttttt
    15661 ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa
    15721 tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa
    15781 cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta
    15841 tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg
    15901 gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag
    15961 gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct
    16021 ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc atcaaaaacg
    16081 gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg ctggcaggtt
    16141 tcgacacagg gcgatccctg aacggcatga atgcgacgaa taagattcag tgcatcgaca
    16201 ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg
    16261 atgccggaac aggctacggc tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct
    16321 ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg
    16381 cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac tttatcgagg
    16441 cagacgcgcg gctcgctggt acggcgtagc gccatacgtg cggcgttcac cgcttttact
    16501 gccgcaatgg cgttacgctc gatgcatggc acctggacct gtccggcgac cgggtcacac
    16561 gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg cgccgggctt
    16621 gcacctaaca gttctgccag accagccgcc gccattgagc acgccacacc aacttcaccc
    16681 tggcagccga cttcagcacc agaaattgac gcgttcatct tataaagaga accaatggca
    16741 ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat gaacttgtcg
    16801 tagtacgcca gcactgccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg
    16861 cgtccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca
    16921 accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca
    16981 gcacggcgtg gaacgcgcag tttgccaggc aacacgcctt cggtggaaat accgcgctca
    17041 ataccgccac gcatgacttc ccatacgttc gccaggtgct gttccagctc ttctttgctg
    17101 tgcagcgcca gctcattttt catcatcagg ccagagagtg acagcccggt ttcctgacaa
    17161 tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctattc
    17221 tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctgg
    17281 ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa
    17341 aggttgtctg catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg
    17401 tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg gatggaatcg
    17461 atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat atcagtgtgg
    17521 tggcctttac cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct
    17581 ttaagcagat tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca
    17641 acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata
    17701 ggaaatacat cgtgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag
    17761 ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg
    17821 ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc
    17881 atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg
    17941 ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg
    18001 ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg
    18061 ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc
    18121 gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac
    18181 agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtaca
    18241 cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc
    18301 ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa
    18361 gagatgatcg gagagtggtt gaaagagaac accataaccg gaattgccag ccacagggtc
    18421 atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg
    18481 ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat
    18541 accagaatac tcattgcttt aacgatcatc tgctcaccga agcgaacgat ggtcatcata
    18601 cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc cagctggtga
    18661 gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag gatcgggtag
    18721 atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct
    18781 acaacctcgg tgatgtcttc gcctgggttt ttaccagaca gtacgaagcg agtcaggccg
    18841 cggtgagcaa aaaatgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata
    18901 ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa
    18961 aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca
    19021 atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct
    19081 tttagccagt tcagctcggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga
    19141 tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta
    19201 tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat tatcccaacg
    19261 ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga
    19321 catctgactt cttccggggc gcgcaatata tgtaggttga actgaaaaaa tcaatacttt
    19381 tagttaatca tggcgataat ttctttttca tagcgataaa atgtggttta tgaaaatagg
    19441 gaaacgtttg cgttacaaca taaattgtat gacatattaa ctgcaatgat atttatcgcg
    19501 tgagggtggt aagaaacatt catataacaa atgaaataaa ttgatgggta tgtgaccaaa
    19561 aacgagaaaa cccggcaaca aacgtcaccg ggggagggag attacgtgca gatttcgtag
    19621 caagggatgt aggctgagcc tggcaacttc atacgatgct gggcaacaaa accctgtagc
    19681 aggtcgtcca tacgacgcat aatctcttta tcgccgttga ttttgtaagg accaaactct
    19741 tcaatggcgc gaataccgac ttcttttacg ttacccgcca caataccgga gaacgcacgg
    19801 cgcagatcgg cagccagcac ttcaacaggt tgatccgggt aaagcttcag attagccata
    19861 ttctcgtgag acggctcaaa cggcatttgc aaatctggcg caatgcgcat tgaccagtta
    19921 aagctgtagg catcgcctgt gtcgcggcga ttttctttca ccagcggcat cgattttttc
    19981 atctgacgag cgacttcagc ggcgtcatca atgatgatgc ggtaatggcg gcgcgcgttt
    20041 tcacccaacg tatgcacgac aaactcgtcc agtacgcgga agtagtcggc gctctctttc
    20101 gggccggtga ggaacaatgg taaaacctga tctttgttgg ccgggttcat taaaattccc
    20161 agcaaataca gcaactcttc tgccgtaccc acaccgccag ggaagataat gatgccgtga
    20221 gcgatacgga caaacgcttc cagacgtttt tcgatatctg gcatgatgat caattcgttg
    20281 accagcgggt taggcggttc agcggcgata atcgacggct ctgtcatacc aataaaacga
    20341 ctgtctttgt aacgctgctg tgcgtgtccg accgcagcac ctttcatcgg cgcttccatc
    20401 gctcccggac cacagccggt gcagatattc agctcacgca ggcccagctg attcccgacg
    20461 cgacgggcat acaaatactc gttttcgtta attgagtgtc cgccccagca gaccaccata
    20521 tttggcgctt caccgacatg cagtgcgcga gcgttacgca agatggaaaa gaccaggtta
    20581 gtgatgtgga cggagttgtc gagattcagg ttgggaaaac gaacggtgtt atggatttgc
    20641 ccgtaaacga agagaatgtc acgcagaacc gcgaacaagt tggcctgcaa cgcgcgaata
    20701 attcgcccat cgacaaaagc ctcttccggg ggattaatca gttccagctt tacgccgcgt
    20761 tcacggcgca ggacgttaat atcgaaattt tcaaaacgag acagcaattc tttgctgtta
    20821 tcggtcaaac taccggagtt cagtacggca agtgaacagt tgcgaaacag ttgatagagg
    20881 tcgctgctgg cggtgcgttt aagcatatcc acttccagct gcgacaacat atccatggag
    20941 ccaagcgggc taatatgtgt aatcaagaaa aactccttat gggacgaata tcgtcattcc
    21001 ctttgattac aatagccctg cctgatgtcc tttcacaacc ttctgcgtga atccagcacg
    21061 caaaattgag aaaaaattta ttgccgaacc agtcttgtgg tcgatgggac aaaatcgcta
    21121 ttactgcgcc acgggttaat gtccagaccg ccacgacggg tataacgtgc gtaaacgctc
    21181 aatttttctg gctggcagaa gcgtaacagg tcattaaaga tgcgttccac gcactgttcg
    21241 tggaactcgt tgtgatgacg gaatgagacc aggtaacgca gcagtttttc tctgtcaatt
    21301 tggcgtccac gatactgaat ttggatcgaa ccccaatctg gttgatgggt gatcaggcag
    21361 tttgatttca gcaggtggct gacaagcgtc tcttccacta ctttttcacc acaggtggca
    21421 ttctccagat agtcagtagt gaattcatag ttatcgatag tgatatcctg gtcatcaatg
    21481 caagtgccat taaaatgacc tatcggctgg ccttccagtt catcaagacg atataacgcc
    21541 acgctaatct taccctgagc gcaagtgctt aagtcgcgct ccagcgtctg gcgcacctca
    21601 tcccagttat taaaacgcgt ctggttaaaa ctgttgagat agagcttaaa actcttcgac
    21661 tcaatcagat ttacgctggt gtaatcaagt tcaacatgac cgacagcgac ctgcggcaaa
    21721 cctttcgcat tcagccagga aagttcatac agcgtccaga tatccgtacc gtgaaaaggc
    21781 aggttatccg ctttcagacc cagcgggtcg cgattcaggc tgcgtggaac gccttgcagt
    21841 aggctggcgt cataggtatc ccggtaatcg gttgattttc caagagtcag gcccgcaagt
    21901 gcctgatggt ttgcataaga agacatgttt caccgtcata aaccagataa actaaacgac
    21961 aagtgtatct ggtttaagtg aagagagaaa ttagtggacg atttgaccgc acaagccctg
    22021 aaagatttta ctgcacgcta ctgcgatgcg tggcatgaag agcataaaag ctggccgtta
    22081 agtgaggaac tgtacggtgt tccttcaccg tgcattattt caactaccga agatgccgta
    22141 tactggcaac cccagccgtt taccggggaa caaaatgtaa acgcggttga acgcgctttt
    22201 gatattgtga tacaacccac aattcatacc ttttatacga ctcagtttgc cggggatatg
    22261 cacgcgcagt ttggcgatat caaactgaca ttgctgcaaa cctggagtga agacgacttc
    22321 cgtcgggtgc aggaaaatct gattggtcat ctggtgactc agaaaaggct aaaactgccg
    22381 cccacgctat ttatcgcgac gctggaagaa gagttagagg tcatctcggt gtgtaatctc
    22441 tccggtgaag tgtgcaaaga gacgctggga acacgtaaac ggacgcattt agcctcaaat
    22501 cttgcggaat tccttaatca acttaagcct cttctgtaat ccaaataccc catctggttg
    22561 tgagagatct cttacagacc ctgtaggaga tcgccaggaa ataagcgaat acttaaaaag
    22621 ataagaatcg ttattttcat ttaaaatcaa aatgttgatt gttaattctt aactttcata
    22681 tgaaattttc cttaaggcat tgtctgtaag cgccttgtaa gacttcgcga aaaagacgat
    22741 tctatcttcg tcgacaggga gtcagacaac gaagtgaaca tcaggatgat gacacttctg
    22801 caggacacac caggatggtg tttcagggaa aggcttctgg atgaagcgaa gaggatgacg
    22861 caggacgcgt taaaggacac ctccaggatg gagaatgaga accggtcagg atgattcggt
    22921 gggtcaggaa ggccagggac acttcaggat gaagtatcac atcggggtgg tgtgagcagg
    22981 aagcaatagt tcaggatgaa cgattggccg caaggccaga ggaaaagttg tcaaggatga
    23041 gcagggagca acaaaagtag ctggaatgct gcgaaacgaa ccgggagcac tgtgaataca
    23101 gtgctccctt tttttattcc tgctatcctt cgcggcagtt tttcttattg aagttgcttt
    23161 atgaccactc atgaccgcgt tcgtctccag ttgcagacgc ttgaagcgtt actgcgtgaa
    23221 catcagcact ggcgaaacga tgaaccccag ccgcatcaat ttaatagtac ccaaccgttc
    23281 tttatggaca ccatggaacc actggagtgg ttgcagtggg tgctgatccc gcgaatgcac
    23341 gatttgttaa ataacaacca gccgttaccg ggcgcttttg ctgttgcacc ctattatgaa
    23401 atggcgctgg cgacggacca tccgcaacgc gcgcttattc tggctgagtt ggaaaaactg
    23461 gatgctcttt ttgcggatga tgcgagctaa tgctggaaat actctatcag gatgaatggc
    23521 tggttgcggt aaataaaccc tccggctggc tggttcaccg tagctggctg gatcgcgacg
    23581 agaaagtagt ggtcatgcaa accgtgcgtg accagatagg acagcatgtt tttactgctc
    23641 atcgtctgga ccgacccact tctggtgtgt tgttgatggg attatccagc gaggccggac
    23701 ggctgctggc acaacagttt gaacagcacc aaatccagaa acgttaccat gcgattgtgc
    23761 gcggctggtt gatggaagaa gcggtgctgg attatccact ggtggaagaa ctggacaaaa
    23821 tcgctgataa atttgcccgc gaagataaag gcccgcagcc agcagtgacg cattatcgcg
    23881 gtctggcgac cgtagaaatg ccggtagcga ccggacgtta cccgaccacg cgctacggcc
    23941 tggtggaact ggagccgaaa accggacgca aacatcagct tcgccgacat cttgcccatt
    24001 tgcgtcatcc gattattggc gatagcaaac atggcgattt acgccagaat cgcagcggtg
    24061 ctgagcattt tggcctccag cggttaatgc tacatgccag tcagttgtca ttgacgcatc
    24121 cttttactgg cgagccgctg actattcacg cgggcctgga cgacacctgg atgcaggcgt
    24181 tatcacaatt tggctggcgc ggtctgcttc ctgaaaatga aagggttgag ttcagtgcgc
    24241 catcgggtca ggatggagag ataagttcct aatccaggga gaaaacggta tggcggaaat
    24301 tggtattttt gtcggcacca tgtacggaaa ttcgctgtta gtggcggaag aagcagaagc
    24361 aattctgacc gcacagggcc acaaagcaac ggtatttgaa gatcctgaat taagcgactg
    24421 gctgccttat caggataagt atgttctggt ggttacgtcc acgaccgggc agggcgacct
    24481 tcctgatagc attgtgccgc tctttcaggg aatcaaagat agtctgggct tccagccgaa
    24541 tctgcgttat ggcgtgattg cgctcggcga cagcagttat gtaaatttct gcaatggcgg
    24601 caaacagttc gatgccttgt tgcaggaaca gagcgctcag cgggttggcg aaatgctgtt
    24661 gattgacgcc agtgagaacc cggaaccgga aacggaatca aatccgtggg tcgaacagtg
    24721 gggcacgctg ttgtcctgat atgttcagcg agcggtaaat gtcgttttag cggtgctgaa
    24781 tcgaatcttt tcaggcaaat gccagtaaaa actgcttcat agcgcggatt tttactggcg
    24841 tttgcctgga gtcaagcgat ccatttcata ctcttcttta tttcttcgtt ttaacccttc
    24901 ctttcttgtt cttgttttca tttccgtgaa gtggattcca ccgtccaggg ctaatgccaa
    24961 aatcgggcct cattgaacgc attaatgttg tgttgttgca cggtgagccg ctatggcgcg
    25021 ctttttatac tgctattgcc agatataaac acgcgccgta ttcggcgaac gacctataaa
    25081 aacggcaaaa aacaccctac gtcacctctg atttcctggc tatgtcgcag tccagagtga
    25141 gcgtggctaa cgcgaatttt caggagtgca acaatgagtt ctttaagtca ggctgcgagc
    25201 agtgtggaaa aacgcacgaa tgctcgttac tggatagtgg tgatgttgtt tatcgtcaca
    25261 tccttcaact acggcgaccg cgctacgctt tctattgccg gttcggaaat ggccaaagat
    25321 atcggccttg atcccgtggg aatgggctat gtgttctctg ccttctcatg ggcttatgtt
    25381 atcgggcaga tccctggtgg ttggttgctg gatcgttttg gttcaaaacg cgtctacttc
    25441 tggtcgatct ttatctggtc gatgtttacc ttgctgcaag gcttcgtcga tatctttagt
    25501 ggattcggca ttatcgttgc cctgtttact ctgcgcttcc tggtcgggct tgctgaagcg
    25561 ccatctttcc ccggcaacag tcacattgtt gcggcctggt ttccggcgca ggaaagggga
    25621 acggcggtgt cgatttttaa ctccgctcaa tacttcgcaa cggtgatctt cgcgccgatt
    25681 atgggctggc tgacgcatga agtgggctgg tcacacgtct tcttctttat gggcggtctg
    25741 gggattgtca tcagctttat ctggttgaaa gtcatccacg agccaaatca acatccgggg
    25801 gtaaatcaga aagagctgga gtacatcgcc gcgggtggcg cgctgatcaa tatggatcag
    25861 caaaacacca aagttaaagt gccgttcagc gtgaagtggg ggcagatcaa acaactgctc
    25921 gggtcacgga tgatgatcgg cgtttatatc ggtcagtact gtatcaacgc cctgacttac
    25981 tttttcatta cctggtttcc ggtttatctg gtgcaggcgc gtgggatgtc gattctgaaa
    26041 gcgggctttg tggcttccgt tccggcggtt tgcggtttta tcggcggtgt gctgggtggg
    26101 attatttccg actggctgat gcgtcgcacg ggatcgctga acattgcgcg taaaacaccg
    26161 atcgtaatgg gcatgttgct gtcgatggtg atggtgttct gcaactacgt caacgttgag
    26221 tggatgatca tcggctttat ggcgctggcc ttcttcggta agggcatcgg ggcgctgggt
    26281 tgggcagtaa tggcagatac cgcgccaaaa gagatcagcg gtctttccgg tggcctgttc
    26341 aacatgttcg gtaacatttc tggcatcgtc acgccaatcg caattggtta tatcgttggc
    26401 acgactggct cctttaatgg ggcgctgatt tatgttggtg ttcatgcctt aatcgcggta
    26461 ctgagctacc tggtgctggt gggcgatatc aagcgtatcg agctgaaacc tgtcgcgggg
    26521 caataagatg acgacacaat ccagtcctgt tattactgat atgaaagtca ttccggtcgc
    26581 cgggcacgat agcatgttgc ttaatattgg tggggcacat aacgcatatt tcacccgcaa
    26641 tattgtggta ctcaccgata acgccgggca taccggcatt ggtgaagcgc cgggcggaga
    26701 ggtgatttat caaacactgg tcgatgctat tccgatggtt ctgggccagg aagttgcgcg
    26761 cctgaataaa gtggttcagc aggtgcataa aggtaatcag gcagccgatt ttgatacctt
    26821 cggcaaaggt gcctggactt ttgaattgcg cgttaatgcc gtggcggcgc tggaagccgc
    26881 cttgcttgac ctgctaggta aggcgctgaa tgttccggtc tgcgaactgt tggggccagg
    26941 caagcaacgc gagactatta ccgtcctcgg ttatctgttt tatatcggtg atcggaccaa
    27001 aaccgatctt ccttatctgg aaaatacgcc gggcaaccat gagtggtatc agttgcgcca
    27061 tcagaaagcg atgaacagcg aagccgttgt gcgtctggcg gaagcctcac aggatcgcta
    27121 tggctttaaa gatttcaaac ttaagggcgg ggtgttacct ggcgagcaag aaatcgacac
    27181 tgttcgtgca ttgaagaaac gcttccctga tgcgcggatt accgttgatc ccaacggtgc
    27241 ctggctactt gatgaagcca tttctctatg caaagggctg aatgatgttc ttacctatgc
    27301 cgaagatcca tgcggcgcag aacagggttt ctccggtcgt gaagttatgg cggagtttcg
    27361 gcgggcgacc ggcttgcccg tcgcgactaa catgatcgcc accaactggc gcgaaatggg
    27421 tcatgcggtg atgctcaatg cggtagatat tccacttgcc gatccgcact tctggacgct
    27481 ttccggtgca gtccgtgtgg cgcagctttg cgacgactgg gggctgacct ggggctgcca
    27541 ttctaataac catttcgata tctctctggc gatgtttacc catgtgggcg cggcggcacc
    27601 gggtaatcct accgctatcg atacccactg gatttggcag gagggcgatt gtcgcctgac
    27661 caaaaatccg ctggagatta aaaacggaaa aattgccgtt cctgatgcgc ccggtctggg
    27721 cgtggaactg gactgggaac aggtacaaaa ggcacatgag gcctataaac gtctgcctgg
    27781 cggtgcgcgt aacgacgcag gtccgatgca gtacctgatc cccggctgga cctttgaccg
    27841 taaacgtccc gttttcggcc gtcattgatt ctgaaaaagg acataaatat gagttctcaa
    27901 tttacgacgt ctgttgttac tgaaatgcag gtcatcccgg tggcgggtca tgacagtatg
    27961 ctgatgaatc tgagtggggc acacgcaccg ttctttacgc gtaatattgt gattatcaaa
    28021 gataattccg gtcacactgg cgtaggggaa attcccggcg gcgagaaaat ccgtaaaacg
    28081 ctggaagatg cgattccgct ggtggtaggt aaaacgctgg gtgaatacaa aaacgttctg
    28141 acgctggtgc gtaatacttt tgccgatcgt gatgctggtg ggcgcggttt gcagacattt
    28201 gatttgcgta ccactattca tgtagttacc gggatagaag cggcaatgct ggatctgctg
    28261 gggcagcatc tgggggtaaa cgtggcatcg ctgctgggcg atggtcaaca gcgtagcgaa
    28321 gtcgaaatgc tcggttatct gttcttcgtc ggtaatcgca aagccacacc tctgccgtat
    28381 caaagccagc cggatgactc atgcgactgg tatcgcctgc gtcatgaaga agcgatgacg
    28441 ccggatgcgg tggtgcgcct agcggaagcg gcgtatgaaa aatatggctt caacgatttc
    28501 aaactgaaag gcggtgtact ggccggggaa gaagaagccg agtctattgt ggcactggcg
    28561 caacgcttcc cgcaggcgcg tattacgctc gatcctaacg gtgcctggtc gctgaacgaa
    28621 gcaattaaaa ttggtaaata cctgaaaggt tcgctggctt atgcagaaga tccgtatggt
    28681 gcggagcaag gtttctccgg gcgtgaagtg atggcagagt tccgtcgcgc gactggcctg
    28741 ccgactgcaa ccaatatgat cgccaccgac tggcggcaga tgggccatac gctctcgtta
    28801 caatccgttg atatcccgct ggcggatccg catttctgga ctatgcaagg ttcggtacgt
    28861 gtggcgcaaa tgtgccatga atttggcctg acctggggtt cacactcaaa caaccacttc
    28921 gatatttccc tggcgatgtt tacccatgtt gccgccgctg caccgggtaa aatcaccgcg
    28981 attgatacgc actggatttg gcaggaaggc aatcagcgct tgaccaaaga accgtttgag
    29041 atcaaaggcg gtctggttca ggtgccggaa aaaccggggc tgggtgtaga aatcgatatg
    29101 gatcaagtga tgaaagccca tgagctgtat cagaaacacg ggcttggcgc gcgtgacgat
    29161 gcgatgggaa tgcagtatct gattcctggc tggacgttcg ataacaagcg cccgtgcatg
    29221 gtgcgttaag acagaaaacc aaaaccggct ctatctggag ccggcttgag actgatgcta
    29281 aacgcaaaat agcctgttgt gctatgtatc aggtctacat cattttttaa ttcattgaat
    29341 ttaattggtt ttgtaggccg gataaggcgt taacgccgca tccggcataa acacaggcac
    29401 tttgtcacca atctgaaacc agcgtcataa aaagccgatt gctactcgac aagacgtccg
    29461 ttacccgaga attttgctgg cttcgcgcgc gacattatcc atctcgtcca acagttccag
    29521 cagctccggc tccaaatctt cttctttagt accgctacgt agctgttgtt cgatcagttg
    29581 gcagagattc ttcatacgcg gcacaccgct atagccgcaa ctgccatgca gtttatgaat
    29641 caaatcaacc aggccttccg ggttttctcc aaccagctgc tcctcaactt tgttgcgaac
    29701 ttcaggcagg aaatcgagta acatttgcag catatcgcgc gctagatcgg tttttcctgc
    29761 tgcctggcgt agtgccagtt gccagtcgag ggtcgcattc gggttcacca caatttcgtt
    29821 gacttcgggc gtcacgacgc gagaggaaat accgctgcca ggcttgtagc gcaacaacaa
    29881 attatgcaat cgctcttctt caatcggttt cgccagataa tcgctcatcc ctgcgccaag
    29941 cagcttctct ttttgcccgg ccattgcatg cgccgttacc gcgataaccg gcgtttgttg
    30001 ctgatggggg agctggtgga tgagctcgca ggcccgaatg ccatccatgt caggcatttg
    30061 aatatccatt aagatcaaat cgaacggcat ctgtttcgcc cgttcaaccg cctgatgccc
    30121 gctatcgcaa agttccacat gttgcaccat atcttccagc aatgcgccga taagtttcag
    30181 gttagcgggg ttgtcatcaa ctgccatgac tgtcattgcc agcttgcttt catcggttac
    30241 aggcaaaagc gtgttttgtt tgtgatgaca aaattccgtc agggcaggca acaggcgcgt
    30301 aggtgttaat ggtttcagca gacacgcgcc gataccatct tgcttgagtt tttctgcatt
    30361 gacttgtgca tggcaaggaa gtgccagcat caggaaatcg gtcatcgata ccgctttcgc
    30421 taatcgctca tgttgcattg ttagcggctc gcggaaggtc accgcgatgc ctaacaacat
    30481 catgtcgtaa tgcgcgggag gcagcgcgga gaacgttggg ctataaacca cttccagcgg
    30541 cgtttcactt aaaatatcca gcgtgcattg cgctgctgcg gagtttggtt cgacataggc
    30601 caggcgctta ccggcgaggc actgggtgga tggcccttcg ataataatgt tcgggttcag
    30661 atcgagatta atgtggaacc agaaagttga accgcgattc ggctggctat ggaacgaaat
    30721 atcgccgccc atttcattaa ccagtttttg tgtaatcacc agccccagac cggtgccacc
    30781 atgacggcgg gaaatactgg catcagcctg tcggaaggcc tggaataagc gcgattgatc
    30841 gcgttcagga ataccaatgc cggtatcccg aatctgcact tcaatctgca ctttggtatt
    30901 actcagcgca cgtttttcta ccagaatatc aatgttgcca ttctcggtga atttaattgc
    30961 attccccacc aggttagtga tgatttgctg taatcgcagt gggtcgccga tcacgttatc
    31021 aggcacgtcg cttttaatat tgagcgtcag ttccaaccct ttatcgtgag aagaatgtgc
    31081 cagcagagta acgacttcat ccagcgtgct gcgtagtggg aatggaatac tttccagaat
    31141 cagcttacct gcttccagtt tcgagaagtc gagaacatca ttaataattg ccagcaaatt
    31201 atttgccgaa cgttcaatcg tattcaggtg atcgcgctgc gttggtgtta attctgtttt
    31261 cagcgtcagg cgggtaaagc caataacacc attcagtggt gtacgcagct cgtgtgacat
    31321 atttgccaga aactcggatt taatacgcgc cgcttcctgg gcgcgctttt tcgccagatc
    31381 taactcaacg ttctgaattt ccatctgctc cagcgtttca cgcagatcgg acgtcgcctg
    31441 gtcgatattg tgctgcatct cttcgtgata agcagccagc gacattgcca tcgagttgat
    31501 accgtttttc agcatatcca gctcgccgag cataaatcct tccacccggc tgtcgagttg
    31561 cccgcgacgg atgcggtcga cggtattcac catgttgcga atcggaccgg ttacatcgcg
    31621 cattaagcgc cagccaaaaa taagcgcaat accgatacaa aacagcatca tcacgctgga
    31681 aataaagatc tctttatatt gctgcaagcg aaccgattta agatccagct ccagcgcaat
    31741 atatcccaac atattttgac tatttttggc atcgctactg ggcgattcgt cgggggagta
    31801 actctcagaa ataatcggcg tgcggaggat cataatatcg ccgtcacgag tgacagtgag
    31861 ctggcgagga aacggcacgt tgctgccgag ctgcattgat gagggatcaa gatgaaaatt
    31921 ggaggtgaca aagagtcggt tattttcatc ataaaccgaa atcgcgcgaa caatatcgga
    31981 atggcgacga tgcagtacgc ttattaactg accgatagat tcgcgatttt gcaggctcat
    32041 gccatattca gtagaaactg caagcggctc aataatgctg gctccggcat cttccagttg
    32101 acgctgcaag tcgttatagc gatgcacaac gaaaaagata ctcagcaata aaccaataag
    32161 gacggtcggt gccaggatca gaatcatcat gcgtgcgcgc aggctgtagt tggtcatgga
    32221 gttccgttat gggacaatta aggtcacact gttaaattga gaaagtctcg ccaatggcgc
    32281 aattctactc tgcaaaacga cgcacgacga cgcgtcagat cataaccgtt tcagtcaacg
    32341 acctcgactc ttttggtcag ggcgtggcgc gacataacgg caaaacgcta tttatccccg
    32401 gattattgcc gcaggaaaac gcggaagtta ctgttactga agataaaaaa cagtatgccc
    32461 gcgctaaagt cgtacgccgg ttaagcgata gcccgggacg cgaaacgcca cgctgtcctc
    32521 attttggcgt atgcggcggc tgtcagcaac aacacgccag cgtggattta cagcagcgaa
    32581 gcaaaagtgc ggcactcgcc cgattaatga aacacgaagt ctctgaagtg atcgccgatg
    32641 ttccctgggg ctatcgccgt cgcgcgcgtt taagtctgaa ctacttaccg aaaacacagc
    32701 aacttcagat ggggtttcgc aaagcgggct ccagtgacat tgtcgacgtt aaacaatgcc
    32761 ccattttagt gccccaactt gaagcattgc tgcccaaagt catggcatgc ttgggcagct
    32821 tacaagttat gcgccatctt ggtcatgttg aactggtaca ggcaaccagc ggcacgctga
    32881 tgattttgcg ccataccgca ccgctaagtt cggcagatcg cgaaaaactg gaacgctttt
    32941 cgcattctga aggcctggat ctgtatctcg cccccgatag tgagatactc gaaaccgtct
    33001 ctggtgagat gccctggtat gactcaaatg ggttgcgctt aacttttagc ccgcgcgatt
    33061 ttattcaggt caatgcgggt gtgaaccaaa aaatggtagc gcgtgcgttg gaatggctgg
    33121 atgtgcaacc tgaagatcgc gtactggatt tgttctgcgg tatgggcaac tttacactgc
    33181 cattggcgac acaagctgcc agtgtggtgg gtgtagaagg cgttccggcg ctggtggaaa
    33241 aaggccagca gaatgcgcgt cttaatggct tacagaatgt gacgttttat cacgaaaatc
    33301 ttgaagaaga tgtcacaaag cagccgtggg cgaaaaacgg cttcgataaa gtgttgctgg
    33361 acccggcgcg agcaggtgcc gcaggtgtta tgcagcaaat tataaaactg gaacctattc
    33421 gtatagttta tgtatcctgt aaccctgcaa cgctggctcg ggatagcgaa gcgttattaa
    33481 aagcaggata taccattgcg cgactggcga tgctggatat gttcccacac acgggacatc
    33541 tggaatcgat ggtacttttc tcgcgcgtta aatagttgcg atttgccgat ttcggcaggt
    33601 ctggtcccta aaggagagga cgatggttgc ggtaagaagt gcacatatca ataaggctgg
    33661 tgaatttgat ccggaaaaat ggatcgcaag tctgggtatt accagccaga agtcgtgtga
    33721 gtgcttagcc gaaacttggg cgtattgtct gcaacagacg caggggcatc cggatgccag
    33781 tctgttattg tggcgtggtg ttgagatggt ggagatcctc tcgacattaa gtatggacat
    33841 tgacacgctg cgggcggcgc tgctgttccc tctggctgat gccaacgtag tcagcgaaga
    33901 tgtgctgcgt gagagcgtcg gtaagtcggt tgttaacctt attcacggcg tgcgtgatat
    33961 ggcggcgatc cgccagctga aagcgacgca cactgattct gtttcctccg aacaggtcga
    34021 taacgttcgc cggatgttat tggcgatggt cgatgatttt cgctgcgtag tcatcaaact
    34081 ggcggagcgt attgctcatc tgcgcgaagt aaaagatgcg ccggaagatg aacgtgtact
    34141 ggcggcaaaa gagtgtacca acatctacgc accgctggct aaccgtctcg gaatcggaca
    34201 actgaaatgg gaactggaag attactgctt ccgttacctc catccaaccg aatacaaacg
    34261 aattgccaaa ctgctgcatg aacggcgtct cgaccgcgaa cactacatcg aagagttcgt
    34321 tggtcatctg cgcgctgaga tgaaagctga aggcgttaaa gcggaagtgt atggtcgtcc
    34381 gaaacacatc tacagcatct ggcgtaaaat gcagaaaaag aacctcgcct ttgatgagct
    34441 gtttgatgtg cgtgcggtac gtattgtcgc cgagcgttta caggattgct atgccgcact
    34501 ggggatagtg cacactcact atcgccacct gccggatgag tttgacgatt atgtcgctaa
    34561 cccgaaacca aacggttatc agtctattca taccgtggtt ctggggccag gtggcaaaac
    34621 cgttgagatc caaatccgca ccaaacagat gcatgaagat gcagagttgg gtgttgctgc
    34681 gcactggaaa tataaagagg gcgcggcagc tggcggcgca cgttcgggac atgaagaccg
    34741 gattgcctgg ctgcgtaaac tgattgcgtg gcaggaagag atggctgatt ccggcgaaat
    34801 gctcgacgaa gtacgcagcc aggtctttga cgaccgggtg tatgtcttta cgccgaaagg
    34861 tgatgtcgtt gatttgcctg cgggatcaac gccgctggac ttcgcttacc acatccacag
    34921 tgatgtcgga caccgctgca tcggggcaaa aattggcggg cgcattgtgc cgttcaccta
    34981 ccagctgcag atgggcgacc agattgaaat tatcacccag aaacagccga accccagccg
    35041 tgactggtta aacccaaacc tcggttacgt cacaaccagc cgtgggcgtt cgaaaattca
    35101 cgcttggttc cgtaaacagg accgtgacaa aaacattctg gctgggcggc aaatccttga
    35161 cgacgagctg gaacatctgg ggatcagcct gaaagaagca gaaaaacatc tgctgccgcg
    35221 ttacaacttt aatgatgtcg acgagttgct ggcggcgatt ggtggcgggg atatccgtct
    35281 caatcagatg gtgaacttcc tgcaatcgca atttaataag ccgagtgccg aagagcagga
    35341 cgccgccgcg ctgaagcaac ttcagcaaaa aagctacacg ccgcaaaacc gcagtaaaga
    35401 taacggtcgc gtggtagtcg aaggtgttgg caacctgatg caccacatcg cgcgctgctg
    35461 ccagccgatt cctggagatg agattgtcgg cttcattacc caggggcgcg gtatttcagt
    35521 acaccgcgcc gattgcgaac aactggcgga actgcgctct catgcgccag aacgcattgt
    35581 tgacgcggta tggggtgaga gctactccgc cggatattcg ctggtggtcc gcgtggtggc
    35641 taatgatcgt agtgggttgt tacgtgatat cacgaccatt ctcgccaacg agaaggtgaa
    35701 cgtgcttggc gttgccagcc gtagcgacac aaaacagcaa ctggcaacca tcgacatgac
    35761 cattgagatt tacaacctgc aggtgctggg ccgcgtgctg ggtaaactca accaggtgcc
    35821 ggatgttatc gacgcgcgtc ggttgcacgg gagttaggca gaaatttgct cgtatctaca
    35881 atgtagattg atatatactg tatctacata tgatagcggt ttgaggaaag ggttatgatc
    35941 cacagtagcg taaagcgttg gggaaattca ccggcggtgc ggatcccggc tacgttaatg
    36001 caggcgctca atctgaatat tgatgatgaa gtgaagattg acctggtgga tggcaaatta
    36061 attattgagc cagtgcgtaa agagcccgta tttacgcttg ctgaactggt caacgacatc
    36121 acgccggaaa acctccacga gaatatcgac tggggagagc cgaaagataa ggaagtctgg
    36181 taatggtaag ccgatacgta cccgatatgg gcgatctgat ttgggttgat tttgacccga
    36241 caaaaggtag cgagcaagct ggacatcgtc cagctgttgt cctgagtcct ttcatgtaca
    36301 acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac gcaatcaaaa ggatatccgt
    36361 tcgaagttgt tttatccggt caggaacgtg atggcgtagc gttagctgat caggtaaaaa
    36421 gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac agttgcccca gaggaattac
    36481 aactcattaa agccaaaatt aacgtactga ttgggtagtg ttactaactc acttctattc
    36541 tggtcacagg tttccacctg acagacccgc aattttcagg acaattcaat gaatcaaatc
    36601 gaccgtttgc tcactattat gcagcgcctg cgcgatccgg aaaacggctg cccgtgggat
    36661 aaagagcaga catttgccac cattgcgcct tacacccttg aagaaaccta cgaagtgctg
    36721 gacgccatcg cccgtgaaga ttttgacgat cttcgcggtg aactgggcga tctgctattc
    36781 caggtggtgt tttacgcgca aatggctcag gaagaagggc gctttgactt taatgatatt
    36841 tgcgctgcta ttagcgataa attagagcgt cgccatccgc atgtttttgc tgatagttct
    36901 gccgaaaaca gtagtgaagt gcttgcccgt tgggagcaaa tcaaaaccga agagcgcgcg
    36961 cagaaagcgc agcattcggc gctggacgat attcctcgta gtttaccggc tttaatgcgg
    37021 gcgcaaaaaa tccagaaacg ttgcgccaac gttggcttcg actggacgac gcttggcccg
    37081 gtggttgata aagtctacga agagatcgac gaggtgatgt acgaggcgcg gcaggctgtt
    37141 gtcgaccagg ctaaactgga ggaggaaatg ggggacctgc tgtttgccac ggttaatctg
    37201 gctcgccatt tagggacgaa agcagaaatc gcattgcaaa aagcgaacga aaaattcgag
    37261 cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg gactggaaat gacaggtgtt
    37321 gacctcgaaa caatggaaga agtctggcaa caggtaaaac ggcaggaaat tgatctctaa
    37381 gggaattacg cggtcaagcg ccatttgtgt cattttttaa atgacaagcg cttgatttgc
    37441 gtcaaaaaca tttaccccaa aggggctatt ttctcactcc tgatttcaat agtgcgctgg
    37501 cgaagaggag ggataatgaa agtttgtggc acaggtcatg ttcgggtata ctgctttccc
    37561 gtcctggtta ttccatcgtc ttttcaacct aacttctcag gttcagcatg acaacgaact
    37621 atatttttgt gaccggcggg gtcgtatcct ctctgggtaa aggcattgcc gcagcctccc
    37681 tcgcagccat tcttgaagcc cgtggcctca atgtgaccat catgaaactg gatccgtaca
    37741 tcaacgtcga tccaggtact atgagcccaa tccaacacgg ggaagtgttc gttactgaag
    37801 acggcgctga aaccgacctg gacctggggc actacgagcg tttcattcgt accaaaatga
    37861 gccgccgcaa caacttcacc acgggtcgta tctactctga cgttctgcgt aaagaacgcc
    37921 gcggtgacta cctcggcgca accgtgcagg ttattccgca catcactaac gcaatcaaag
    37981 agcgcgtgct ggaaggtggc gaaggtcatg acgtagtact ggtagaaatc ggcggtacag
    38041 taggtgatat cgaatccttg ccgttcctcg aagcgattcg ccagatggct gttgaaattg
    38101 gccgtgagca cactctgttt atgcacctga cgctggtgcc gtacatggca gcgtctggtg
    38161 aagtcaaaac caaaccgact cagcactctg taaaagagct gctctccatc ggtatccagc
    38221 ctgacatcct gatttgtcgt tcagatcgcg ctgttccggc gaacgaacgt gcgaagattg
    38281 cattgttctg taatgttccg gaaaaagcgg ttatttctct gaaagacgtc gattccatct
    38341 ataaaattcc gggcctgttg aaatctcagg ggctggacga ttatatttgt aaacgattca
    38401 gcttaaactg cccggaagca aatctgtccg aatgggaaca ggttatcttc gaagaagcga
    38461 atccggtaag tgaagtcacc atcggtatgg tcggcaagta cattgaactg ccggatgctt
    38521 ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa gaatcgtgtc agcgtcaaca
    38581 tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt tgaaatcctt aaaggtctgg
    38641 acgcaatcct cgtacctggc ggtttcggct atcgtggcgt agaaggcatg attacgaccg
    38701 cgcgttttgc gcgtgagaac aatattcctt atctgggcat ttgcctgggt atgcaggtgg
    38761 cgttaattga ttacgctcgc catgttgcca acatggagaa cgccaactct acggaatttg
    38821 tgccagactg taagtacccg gttgtggcgc tgattaccga gtggcgcgat gaaaacggca
    38881 acgttgaagt tcgtagcgag aagagcgatc tcggcggtac catgcgtctc ggcgcacagc
    38941 agtgccagtt ggttgacgat agcctggttc gccagctgta caatgcgccg acaattgttg
    39001 agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt gaaacagatt gaagatgcag
    39061 gtctgcgcgt tgcgggccgt tccggggatg atcagttggt cgagatcatc gaagttccga
    39121 atcacccgtg gttcgtggct tgccagttcc atccggagtt tacttctact ccacgtgatg
    39181 gtcacccgct gtttgcaggc tttgtgaaag ctgccagcga gttccagaaa cgtcaggcga
    39241 agtaagtaaa aaagttagaa cggcaacgcg taccttgggt acgcgttgtt tgtctggagt
    39301 ttcagtttaa ctagtgactt gaggaaaacc taatgtccaa aatcgtaaaa atcatcggtc
    39361 gtgaaatcat cgactcccgt ggtaacccga ctgttgaagc cgaagtacat ctggagggtg
    39421 gtttcgtcgg tatggcagct gctccgtcag gtgcttctac tggttcccgt gaagctctgg
    39481 aactgcgcga tggcgacaaa tcccgtttcc tgggtaaagg cgtaaccaaa gctgttgctg
    39541 cggtaaacgg cccgatcgct caggcactga ttggcaaaga cgctaaagat caggctggca
    39601 ttgacaagat catgatcgac ctggacggca ccgaaaacaa atccaaattc ggcgcgaacg
    39661 caatcctggc tgtatctctg gctaacgcca aagctgctgc tgccgataaa gggatgccgc
    39721 tgtacgagca catcgctgaa ctgaacggca ctccgggcaa atactccatg ccggttccga
    39781 tgatgaacat catcaacggt ggtgagcacg ctgacaacaa cgttgatatc caggaattca
    39841 tgattcagcc ggttggcgcg aaaactgtga aagaagccat ccgcatgggt tctgaagttt
    39901 tccatcacct ggcaaaagtt ctgaaagcga aaggcatgaa cactgcagtt ggtgacgaag
    39961 gtggctatgc gccgaacctg ggttccaacg ctgaagctct ggctgttatc gctgaagctg
    40021 ttaaagctgc tggttatgaa ctgggcaaag acatcactct ggcgatggac tgcgcagctt
    40081 ctgaattcta caaagacggt aaatacgttc tggctggcga aggcaacaaa gctttcacct
    40141 ctgaagaatt cactcacttc ctggaagaac tgaccaaaca gtatccgatc gtatctatcg
    40201 aagacggtct ggacgaatct gactgggacg gtttcgcata ccagaccaaa gttctgggcg
    40261 acaaaatcca gctggttggt gacgacctgt tcgtaaccaa caccaagatc ctgaaagaag
    40321 gtatcgaaaa aggtatcgct aactccatcc tgatcaaatt caaccagatc ggttctctga
    40381 ccgaaactct ggctgcaatc aagatggcga aagatgctgg ctacactgca gttatctctc
    40441 accgttctgg cgaaactgaa gacgctacca tcgctgacct ggctgttggt actgctgcag
    40501 gccagatcaa aactggttct atgagccgtt ctgaccgtgt tgctaaatac aaccagctga
    40561 ttcgtatcga agaagctctg ggcgaaaaag caccgtacaa cggtcgtaaa gagatcaaag
    40621 gccaagcata agactgactt tatctgattt aaaaatgcca gcctc
//
LOCUS       DAJTXQ010000020        39811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000020 DAJTXQ010000000
VERSION     DAJTXQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.020"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..435
                     /locus_tag="OO515_001033"
     CDS             4..435
                     /locus_tag="OO515_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6575024.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OO515_001034"
     CDS             518..1420
                     /locus_tag="OO515_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6575025.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO515_001035"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO515_001035"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6575026.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO515_001036"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO515_001036"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6575027.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO515_001037"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO515_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6575028.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OO515_001038"
     CDS             complement(5330..6037)
                     /locus_tag="OO515_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6575029.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO515_001039"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO515_001039"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6575030.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OO515_001040"
     CDS             complement(6895..7098)
                     /locus_tag="OO515_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6575031.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7603..7923
                     /gene="cyaY"
                     /locus_tag="OO515_001041"
     CDS             7603..7923
                     /gene="cyaY"
                     /locus_tag="OO515_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6575032.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7963..10509)
                     /gene="cyaA"
                     /locus_tag="OO515_001042"
     CDS             complement(7963..10509)
                     /gene="cyaA"
                     /locus_tag="OO515_001042"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6575033.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10896..11837
                     /gene="hemC"
                     /locus_tag="OO515_001043"
     CDS             10896..11837
                     /gene="hemC"
                     /locus_tag="OO515_001043"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6575034.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11834..12574
                     /gene="hemD"
                     /locus_tag="OO515_001044"
     CDS             11834..12574
                     /gene="hemD"
                     /locus_tag="OO515_001044"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6575035.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12596..13837
                     /gene="hemX"
                     /locus_tag="OO515_001045"
     CDS             12596..13837
                     /gene="hemX"
                     /locus_tag="OO515_001045"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6575036.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            13840..15036
                     /gene="hemY"
                     /locus_tag="OO515_001046"
     CDS             13840..15036
                     /gene="hemY"
                     /locus_tag="OO515_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6575037.1"
                     /translation="MLKVLLLFVLLIAGVVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15698..15859
                     /locus_tag="OO515_001047"
     CDS             15698..15859
                     /locus_tag="OO515_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575038.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15943..17178)
                     /locus_tag="OO515_001048"
     CDS             complement(15943..17178)
                     /locus_tag="OO515_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6575039.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17325..17401)
                     /locus_tag="OO515_001049"
     tRNA            complement(17325..17401)
                     /locus_tag="OO515_001049"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17365..17367),aa:Pro,seq:tgg)
     gene            complement(17444..17530)
                     /locus_tag="OO515_001050"
     tRNA            complement(17444..17530)
                     /locus_tag="OO515_001050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17494..17496),aa:Leu,seq:cag)
     gene            complement(17551..17626)
                     /locus_tag="OO515_001051"
     tRNA            complement(17551..17626)
                     /locus_tag="OO515_001051"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17591..17593),aa:His,seq:gtg)
     gene            complement(17685..17761)
                     /locus_tag="OO515_001052"
     tRNA            complement(17685..17761)
                     /locus_tag="OO515_001052"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17725..17727),aa:Arg,seq:ccg)
     gene            complement(17862..19247)
                     /locus_tag="OO515_001053"
     CDS             complement(17862..19247)
                     /locus_tag="OO515_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6575040.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19438..20178)
                     /gene="rffM"
                     /locus_tag="OO515_001054"
     CDS             complement(19438..20178)
                     /gene="rffM"
                     /locus_tag="OO515_001054"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6575041.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20181..21533)
                     /gene="wzyE"
                     /locus_tag="OO515_001055"
     CDS             complement(20181..21533)
                     /gene="wzyE"
                     /locus_tag="OO515_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6575042.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21530..22609)
                     /gene="rffT"
                     /locus_tag="OO515_001056"
                     /gene_synonym="wecF"
     CDS             complement(21530..22609)
                     /gene="rffT"
                     /locus_tag="OO515_001056"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6575043.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22606..23856)
                     /gene="wzxE"
                     /locus_tag="OO515_001057"
     CDS             complement(22606..23856)
                     /gene="wzxE"
                     /locus_tag="OO515_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6575044.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23858..24988)
                     /gene="rffA"
                     /locus_tag="OO515_001058"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23858..24988)
                     /gene="rffA"
                     /locus_tag="OO515_001058"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6575045.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24993..25667)
                     /gene="rffC"
                     /locus_tag="OO515_001059"
                     /gene_synonym="wecD"
     CDS             complement(24993..25667)
                     /gene="rffC"
                     /locus_tag="OO515_001059"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6575046.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25645..26526)
                     /gene="rfbA"
                     /locus_tag="OO515_001060"
     CDS             complement(25645..26526)
                     /gene="rfbA"
                     /locus_tag="OO515_001060"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6575047.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26545..27612)
                     /gene="rffG"
                     /locus_tag="OO515_001061"
     CDS             complement(26545..27612)
                     /gene="rffG"
                     /locus_tag="OO515_001061"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6575048.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27612..28874)
                     /gene="wecC"
                     /locus_tag="OO515_001062"
     CDS             complement(27612..28874)
                     /gene="wecC"
                     /locus_tag="OO515_001062"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6575049.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28871..30001)
                     /gene="wecB"
                     /locus_tag="OO515_001063"
     CDS             complement(28871..30001)
                     /gene="wecB"
                     /locus_tag="OO515_001063"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6575050.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30057..31103)
                     /gene="wzzE"
                     /locus_tag="OO515_001064"
     CDS             complement(30057..31103)
                     /gene="wzzE"
                     /locus_tag="OO515_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6575051.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31115..32218)
                     /gene="wecA"
                     /locus_tag="OO515_001065"
     CDS             complement(31115..32218)
                     /gene="wecA"
                     /locus_tag="OO515_001065"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6575052.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32458..33717)
                     /gene="rho"
                     /locus_tag="OO515_001066"
     CDS             complement(32458..33717)
                     /gene="rho"
                     /locus_tag="OO515_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6575053.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33802..33903)
                     /gene="rhoL"
                     /locus_tag="OO515_001067"
     CDS             complement(33802..33903)
                     /gene="rhoL"
                     /locus_tag="OO515_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6575054.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34044..34373)
                     /gene="trxA"
                     /locus_tag="OO515_001068"
     CDS             complement(34044..34373)
                     /gene="trxA"
                     /locus_tag="OO515_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6575055.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34504..35769
                     /gene="rhlB"
                     /locus_tag="OO515_001069"
     CDS             34504..35769
                     /gene="rhlB"
                     /locus_tag="OO515_001069"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6575056.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35903..37387
                     /gene="gppA"
                     /locus_tag="OO515_001070"
     CDS             35903..37387
                     /gene="gppA"
                     /locus_tag="OO515_001070"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6575057.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37434..39455)
                     /gene="rep"
                     /locus_tag="OO515_001071"
     CDS             complement(37434..39455)
                     /gene="rep"
                     /locus_tag="OO515_001071"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6575058.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9870 a        10831 c        10047 g         9063 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctgctt
     7141 tctgctttct gctttctgct ttctgctttc tgctttctat catcgcagga gaagacgtaa
     7201 aagcaagcgt ttagcgactc tttatgaagt ctgaaaagtg aggaaatgct gcgagtataa
     7261 cgcttactcg tggggattta tgttttgctg gtagtttcgt gatcggcacc tcgcgccagg
     7321 atggctctat gcactatcgc aatgtggttt gtttgttgtc ttttttagtt ccgtcagcgt
     7381 gaggatgctc gctcggtaaa cctgatcata atgtttacca gaaacaggga aaggcgtggt
     7441 gtaactgcgc cctaatgaag agacaaatct ttcgctacac agagacatca ttattccggt
     7501 gacggatgaa tcgtgccgga acgcagccgc agaggtgcaa actttgaagc cctgacccca
     7561 tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga cagtgaattt
     7621 catcgcctgg ctgatcaact ctggctgacc attgaagaac gcctggacga ctgggatggc
     7681 gacagcgata tcgactgcga aatcaacggc ggcgtgctga ccattacctt tgaaaacggc
     7741 agcaaaatca ttatcaaccg ccaggaaccg ctgcaccagg tatggctggc aaccaaacaa
     7801 ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgcag cggcgaaacc
     7861 ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt cagtttccgc
     7921 taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa aatattgctg
     7981 taatagcggc gtatcgtgat cctgattggc aggcggcatg ttaccgatag attttgtgcg
     8041 gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact gcggcaggtt
     8101 gaagttgatg aagcttgagc cgtaggtgaa gcggtcgtgc gatgacgagt agaagcgact
     8161 gacgtcacgc accagttcct ctttgctgcc ttcgcagtgg tgatacacct caacccggtt
     8221 gctttcgtca agaatgtaga tattaaagcc attctcgtct tgcgtttctt cgaagaagaa
     8281 ctggatgatc ccttcactag caaagccgtc caccaccgcc ggtaatttga cgtgattggt
     8341 ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atagcgccat aaaactcgat
     8401 ggcgttctcc agcttctgta ccgatacatt caggcgttcg aagaacaagc cccaggtttg
     8461 accagaaacg cgcagcgcct tgaaacgccc ggtttcctgg cgggtgctgg aaaggcgcag
     8521 ttcaatacac tcagaaacca gttgctgcac gcgagtacgg attaacccgc gcagatgctg
     8581 gctataacag aagacttcca cgctatctgg cggtgcggcg tcctgatgca ttttgccgag
     8641 aatagttttc agggcttcga tcatcgattg ctcgccgttg aagtgcagcg tacgcacttc
     8701 gttccacgag ttgcggtaca gcaggtcaac gctacctacc aggcaatttt gattctcgcc
     8761 aaagctgaag acatccagct tacggaaatc aaaatgcacc acctgattgc ggaacgccgc
     8821 tgtcgggtca tattccaggt taacgataat cgccagatgg cggatctcac acgggctgta
     8881 gagcgccttc ggtgtcggtg caggtaagcg cagcgggaaa tggtgcgaca cgtcggcgac
     8941 catctcctgc aacttaggca aatcgacaat gccgttacct ttaatataca aacgggtgcg
     9001 cgaggtcagc agaccgttaa accatgccca cgccaccagt ttattcaggt aacggttata
     9061 ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat acagatacca
     9121 acctgaacgg ttagcccggc ccggcggcac ataaataaag gtcagattcg gttccgagag
     9181 atcgggtgaa atctgcgggt ttaccagcgt cactttacct ggtaatgctt caaacgcggc
     9241 atacagctta cgcgtcagca cgccgatatc ctgcggactg gcggagacgc taaggttatt
     9301 gcgacgcgca aagcggatca gattacggta gctctgcatc atcgcgtcga gcaactcgtt
     9361 gtgcgcctca cgcacctgat caatcttcca gttagcacgg ttatcgagca ttgccagacg
     9421 agcttcgtcc caaccccact cgctcactaa ctggctcaac actgcgcgac gccagcctac
     9481 acaggcgcgt tcacggctga gtttttcgca cacttttaaa tagaagcagc gacgtactaa
     9541 atccagacgg gtgaaatctt caatcgccgt caggtattca gtaacacgct ccagcatcat
     9601 gcagtatgga tcgaggccaa acgatacaat ctcgccgtcg tgcaaacgct gtttgatatc
     9661 tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca gcagtgtttt
     9721 cagcaccgct ttgtatgggg aatcgatact cttgtagagc tgccaaaggc tggcaccaaa
     9781 gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat ttggcgtcag
     9841 cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgctcttctt cgtcgcacgg
     9901 caccatattc cacagaatac gcttaccggc gagacgcacg gcggtacgat aaaattcgtc
     9961 aagcagcagt atatgctggg tggagccaca atcttcgccc cccaggctgc cgctttcatt
    10021 atgacgaaag cggttttcat caatcaggaa gaagctgact tccacaccca gcgaggcggc
    10081 ccagctttcc agcaggctgc atttacgttg tagtaactgg cgctcttcgc tatcgagcca
    10141 ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta cggacgaggt
    10201 gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggtggat cctgtactga
    10261 cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat caggcgtgta
    10321 aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg gatggtgata
    10381 gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca tagcagcaag
    10441 cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag tctcaatata
    10501 gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc gccaacatca
    10561 acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta ccacttttcg
    10621 cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca atttaacacc
    10681 ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt ttattctctg
    10741 taaattcctt attacgacag cgtgaaacgt ctgtcaggat ccactgccag acctcatttt
    10801 acggtttgcg caggcgtcta cgtttcacca caacactgac atcactctgg caaggatgtt
    10861 aggatggacc acggatgata atgacggtaa caagcatgtt agacaatgtt ttaagaattg
    10921 ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac aagttgatgg
    10981 cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc gatgtgattc
    11041 ttgatacgcc gctggcgaaa gtaggcggaa aaggcttatt tgtaaaagag ctggaagtcg
    11101 cgctcctcga aaatcgcgcc gatatcgccg tacattcaat gaaagatgtg ccggttgaat
    11161 tcccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc gatgcctttg
    11221 tgtccaataa ctatgacagt ctggatgcgt taccagcagg cagtatcgtc gggacgtcca
    11281 gtttacgtcg ccagtgccaa ctggctgaac gccgcccgga tctgattatc cgctccctgc
    11341 gcggcaacgt cggcactcgc ctgagcaaac tagataacgg cgaatacgat gccatcattc
    11401 ttgccgtagc cggactaaaa cgtttaggtc tggagtcccg cattcgcgcc gcgttaccac
    11461 ccgagatttc tcttccggcg gtaggacaag gtgcagtcgg tattgaatgc cgccttgatg
    11521 atacgcgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca ctgcgcgtta
    11581 ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtgcca attggtagct
    11641 acgccgagct tattgatggc gaaatctggc tgcgtgcgct ggtcggcgcg ccggacggtt
    11701 cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaaa tgccaaacaa atggggattt
    11761 cgctggcaga agagctactg aataacggcg cgcgcgagat cctcgctgaa gtctataacg
    11821 gagacgcccc ggcatgagta tccttgtcac ccgcccgtct cccgctggag aagagttagt
    11881 gagccgtctg cgcacactgg ggcaggtggc ctggcatttt ccgctgattg agttttctcc
    11941 gggtcaacaa ttaccgcaac ttgctgatca actggcagcg ctgggggaga gcgatctgtt
    12001 gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctacatc agcaagatcg
    12061 taaatggccc cgactaccta attatttcgc cattggacgc accaccgcac tggcactaca
    12121 taccgtaagc ggacagaaga ttctctaccc gcaggatcgg gaaatcagcg aagtcttgct
    12181 acaattacct gaattacaaa atattgcggg caaacgtgcg ctgatattac gtggcaatgg
    12241 cggtcgtgag ctaattgggg ataccctgac ggcgcgcggt gctgaggtca ctttttgtga
    12301 atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc gctggcaatc
    12361 ccgcgaggtg acgacggtcg ttgttaccag cggtgaaatg ttgcagcaac tctggtcgct
    12421 gatcccacaa tggtatcgtg agcactggtt actacactgt cgactattgg tcgtcagtga
    12481 gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaaggtcg ccgataacgc
    12541 tgccaacgat gcgcttttac gggcattaca ataactctca taacaggaag ccataatgac
    12601 ggaacaagaa aaaacctccg ccgtggttga agagactagg gaggccgtgg acaccacgtc
    12661 acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc tcagcgcggc
    12721 ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta aacaacaggc
    12781 cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat tgcaaaaagc
    12841 ccaggagagc caaaaagccg aactggaagg cattattaag caacaggcta cgcagcttga
    12901 acaggcgaat cgtcagcaag aaacgctggc aaaacagctg gatgaagtcc agcaaaaggt
    12961 cgccaccatt tccggcagcg atgctaaaac ctggctgctg gcccaggccg attttctggt
    13021 gaaactcgcc ggacggaagc tgtggagcga tcaggacgtc acgaccgctg cagcgttgct
    13081 gaaaagtgca gacgccagcc tggcggatat gaatgacccg agtctgatta ccgttcgtcg
    13141 ggcaattacc gatgatatcg ccagcctttc tgcagtatcg caggtggatt atgacggcat
    13201 catccttaag cttaatcagc tttcaaatca ggtagataac ctgcgtctgg ccgataatga
    13261 cagcgatggt tcgccgatgg attcagacgg tgaagagctt tccagttcca tcagcgaatg
    13321 gcgtatcaat ctgcaaaaaa gctggcagaa ctttatggac aacttcatta cgattcgccg
    13381 tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc tgcgcgaaaa
    13441 tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcatcagg aagagactta
    13501 tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata ctgatgatgc
    13561 caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata tctcgatgga
    13621 tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga cccgcgtgcg
    13681 taacctgctg gcacaaccgg cagcggggac aacggaagct aaacctgcac ctgcacctgc
    13741 acctgcacct gcacctgcac ctgcacctgc acctgcacct gcacctgcac ctgcaccgca
    13801 aggtgatact ccggcagccg cgccgcaagg agaataacca tgctaaaagt gttattactc
    13861 tttgttttgc tgattgcggg ggtagtggtt ggcccgatga ttgccggtca tcagggttac
    13921 gtgctgatcc agactgacaa ctacaatatc gaaaccagcg tcacgggcct ggcgattatt
    13981 ttgattctgg cgatggtagt gctgtttgcc attgagtggc tactgcggcg gatcttccgc
    14041 acaggcgcac acacccgtgg gtggtttgtc ggacgtaagc gtcgccgtgc ccgtaagcag
    14101 accgaacagg cgctgctgaa actggcggaa ggcgattatc agcaagttga aaagctgatg
    14161 gcgaaaaatg ccgatcacgc ggaacaaccg gtggtgaact atctactggc tgccgaagcc
    14221 gcgcaacaac gtggtgatga agcacgcgcc aaccaacatc tggaacgcgc agcagagctg
    14281 gccggcaatg acaccattcc ggtagaaatc acccgtgtac gtctgcaact ggcccgtaat
    14341 gaaaaccatg ctgcacgcca cggcgtggat aagctgctgg aagttacgcc acgccatccg
    14401 gaagtattac gtctggcgga acaggcgtat atccgcacag gtgcatggag ttctctgctg
    14461 gatatcatcc catcaatggc gaaagcccat gttggtgatg aagaacatcg tgcaatgctg
    14521 gaacaacagg catggattgg cctgatggat caggcgcgtg ccgataacgg tagtgaaggt
    14581 ttgcgtaact ggtggaaaaa ccaaagccgg aaaacgcgtc atcaggtagc gttgcaggtg
    14641 gcaatggcgg aacatcttat tgaatgtgac gatcatgata ctgcccagca aattatcatc
    14701 gatggcctga aacgccagta cgacgatcgc ctactgctgc cgattcctcg actgaaaacc
    14761 aacaatccgg aacagctgga aaaagtgctg cgccagcaga tcaaaaacgt cggcgatcgc
    14821 ccgctgttgt ggagcacact gggccagtca ctgatgaagc acggagaatg gcaggaagca
    14881 tcgctcgcct tccgcgcagc gctgaaacaa cgtccggacg cctacgatta cgcatggctt
    14941 gccgacgcgc tggacagact gcacaagccg gaagaagctg cagcgatgcg tcgcgacggt
    15001 ttgatgttaa cgttgcagaa caacccacca cagtagttcc ttctcacccg gaggcaagca
    15061 cctccgggac ctttctgatg cataaaaaaa cgcctgctct tattacggag caggcgttaa
    15121 aacaggtctg tatgacaaca gtgggtgctt cactcaacgt tgtgtccatg gtgtctgatg
    15181 aggcataagc gacatctgtc agtggacgat aagcaccgta aacggctctg cgtcattccg
    15241 gagtttatga ggcactaagg cgaacataag agatggaatg agcatctacc cgtttattat
    15301 gccacagaga atcgggaaat aacatccctt aacacttgtt atgagataat tctgtaatcc
    15361 tctttgcttc ctgagtgaat atttaacctg agcttgatcc tacacatact tattatgaat
    15421 gataaaattc attcaattaa taacacatat attaattgcc gttaaaacta aaaaagcatt
    15481 aataatcaac gcaatataat aaacccgcct tacatatcaa ctgcgccaga ggtaggattg
    15541 aaaacgctct cctgattttc caattcattt tctggataaa taaataattt atttttgtca
    15601 ctattattta tgtaatcatc ctgtcaggga gagggatctc aattatcaat gcttaattac
    15661 gtcatcattt tgatgacatg aaaccggagc attttgcatg gaattttcgt tttcacccaa
    15721 acttcttgtt gttgctgtcg ccgccgctct tcctctcatg gccagcgcag cagatacccc
    15781 gtcaactgcc accgcacgca aaggctttgc cggatacgat cacccaaacc agtatccaca
    15841 gaacgctgca atttattgaa tttgcacgat catgtaggcc ggataaggcg tttacgccgc
    15901 atccggcaat caaccgcagg cggccacaaa atggccgctt ttttacttat tcaccaccag
    15961 caaatgtgcc tgcataatgt cactggccgg gcgaccgtgc gccagcaaat cagccattgc
    16021 tttaagatat ggcggtagat ggcggaaata acgctgatac ccggcacaca aataattcag
    16081 ccccggtttg ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc cccagcacgc
    16141 ttttaacacg ttacaactgc gacactgcgc cggtaattgc ttaaatttat cttcaccaaa
    16201 cacctgctgt tgcggggaat cgatcatttc tgcaattgtt tgctgatgca tattccccag
    16261 ccgatattgc ggataaacat agtgatcgca ggcgtaaacg tcgccgttgt gctcaacaat
    16321 caccgagcgc ccacaggttg gctgatgatg gcaaaccgca cccggcgcac cgacaaaatt
    16381 ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt tgatccagtg
    16441 gtcgaatatc gccaccagaa attcaccaaa ttcatcgggg cgcaccgacc attccgtcag
    16501 ttccccctga atatcaccag gcgcatgcaa tttcagtcca tcgcgggcag ctgtttcatc
    16561 agccagacgc tcgaccaccg gaataaactg gatgaattcg actcccgcat cgcacaaaaa
    16621 atcataaacc tgcaacggtt gctgcgcgct ggtgcggttg acgcagacca gcacgttata
    16681 gtcgacatga tgtttttgca gcagcgtcag ggcacgcatc accagcttat gcgttggtct
    16741 gccacctttg gtcacgcgat attgattgtg gatctcagcc ggaccgtcca gcgataaccc
    16801 aacaagaaaa tgattttctg ccagaaatgc acaccattta tcatcgagca gcacgccgtt
    16861 agtctggaag ctgttactta tcttcctgcc agcaccgtat ttcgcctgta actttacggc
    16921 acggcggtaa aaatccagcc cgagtaacgt tggttcgccg ccctgccagg taaaagccac
    16981 ttcgttttgc ggttcgctgg cagcgatata gtgacggaca tacgcttcca gcgtgtcatc
    17041 gtccatatgc gtgactggct tttcgcggta aagggattgt ttttcgagat aaaaacagta
    17101 gtcacagttc agattacaat cggaaccact cggtttcgcc atcacatgaa aagcacgcgt
    17161 tggaacctgt tgcagcatgg tcactccttg cctgaagtca tccttcaata cgttaagggc
    17221 gaaatcatac cgtggggcaa tgagttatta cgggcgttta ttgcgaaggg agggtgacga
    17281 aatgcacaga aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg gcgagagagg
    17341 attcgaacct ccgacccact ggtcccaaac cagttgcgct accaagctgc gctactcgcc
    17401 gaaatactgc tttttgaatt tttagttcaa ttctttaaag tcgtggtgcg agggggggga
    17461 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
    17521 ccaccttcgc attgtcacaa cttctaataa tggggtggct aatgggattc gaacccacga
    17581 caactggaat cacaatccag ggctctacca actgagctat agccaccact acaaatcttg
    17641 ttacgcggta ttactaccac cgcagctcaa gcgccgggac taaatggcgc gcccgacagg
    17701 attcgaacct gagacctctg cctccggagg gcagcgctct atccagctga gctacgggcg
    17761 cttagcgccg ttgcgggggg ataatacgga cttcccaccc ctctgtctag tcccttttta
    17821 aataaattgc gcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc
    17881 gctttcccgt ggcgattaag gccaaaaact ttataaatcg ccgtcaccgc cagcataaag
    17941 atgatgccaa caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg
    18001 caaatcagga atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga
    18061 tggctggcaa tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca
    18121 aaccatggca ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc
    18181 ggattgggaa tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc
    18241 acacccgcaa ctggtacgcc gtgacgggaa actttcgcca tcgccgccgg taactgacgg
    18301 tttttcgcca gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag
    18361 agcgcagccg tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg
    18421 gcaaaagtca gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg
    18481 acgataacga aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctaca
    18541 gcactgcgca gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc
    18601 acgccctggt aggacgccac cacaatacac agcgcggtca ggaacccttt ccagccaccc
    18661 gcaaagaaac cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca
    18721 aagccaaaga aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg
    18781 atcatcgcga accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac
    18841 gccaccagcg ccaccgcgat caatgcaggt atccactgcg ccatctccgg gaaccagaac
    18901 tggacataaa cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag
    18961 taagaccagg cggtgagata accaaaaaac gggctcatat aacgatgcgc ataaacggcg
    19021 aacgaaccgg taacaggttc aaggaacaac atttcgccca ttgaacgcat gatgaaaaag
    19081 acgaacagcc cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta
    19141 ctggcggccc ccataaacag gccgacgcca atagtgcccc ccagggcgat gagttcgata
    19201 tgtcgagctt ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt
    19261 gtgtttgcat gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat
    19321 gttttcgtaa ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa
    19381 taaaaaagca gacaggcgac ggagtgacca ctccgtcgct ttacaaagag aggaaaatca
    19441 gaggttgccg gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg
    19501 gctcggctgc gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg
    19561 tgcgcgtttt acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc
    19621 atctggatgc accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc
    19681 aacggtgacg atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc
    19741 gggtttaaaa taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt
    19801 agcttcagtt tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc
    19861 tttgcctgcg cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg
    19921 cgcctgcggg tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa
    19981 ttcggcagcg ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc
    20041 agcattaatg gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag
    20101 ggcgtgctgc atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc
    20161 cgtggtgttg ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt
    20221 ttatgaatga gtccggcgct ttcaaaaagc cagtacagca gttttgcgat catcagacat
    20281 gcgccgaaga ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgc
    20341 gccagcacga tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta
    20401 tagcgattag gctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcaga
    20461 ccaaccacga tcgccccgag cgggatgaac aacgcgccgc ccatcaccac cagtgagcct
    20521 ataagcgtag gcgagatcgc cagtccggag tggttattca gcacttccca ggtaaagtag
    20581 ttggctgagt tcagcaccat actcgggcga cccggccaca gccaggaagg gataaagaca
    20641 tagaaatcgc ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac
    20701 agcaacgcca gattctccca cggggagaag gtgtcgcgag tgagatagag aaacgtatag
    20761 aacgcttcat cgccgctcac attcattcca tagcgtttta gcgccagcca gaacatgcca
    20821 acaatcccca gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg
    20881 ccaataaaca ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc
    20941 atataagtca gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg
    21001 tcctggcgca gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt
    21061 aacgccacgc cggagacttc actggagaag atctgactgt aggagttaag ccggaacagc
    21121 aaaaagccgt tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc
    21181 acccacgtaa gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca
    21241 gcaacgcgtt tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc
    21301 gcagaaagca acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat
    21361 accagcacgc tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag
    21421 aagacattga agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac
    21481 agcgtgcaga gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct
    21541 ccccggcggc aatagccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg
    21601 caatggtgtt tttatccacc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt
    21661 tgagatcgtc ggtagtaaac agcaccggca aatgctgttc cgtcatatcc tgccagaacg
    21721 gattttcccg gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa
    21781 tgccctgctg gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat
    21841 aggcgtcaaa ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca
    21901 gccccgcctg acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca
    21961 ccaccacttt taccgtatcg ccaaattgct gatgaacggc gcgcaaggca gcaatatggt
    22021 cattgctgcg gtcaccggag ttccccacca gaatggtcat tttcccttca cgttgccgat
    22081 cattcgccat cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttcgcccc
    22141 gcacctttgg atgcgttttg gcaaaaaagc tcaaatcacc gcgggtggca aatacacagc
    22201 cgactcgctt ttgcgccagg cgacgtagcg ggtaaaaaag cttatatctc aagccactgg
    22261 aaagctcata caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac
    22321 cactcagcag agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct
    22381 gacggttagc ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa
    22441 attgcacaga aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa
    22501 actcgcgcgc gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc
    22561 ggttatggtg agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg
    22621 ccagagtaaa aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc
    22681 ctgcgccgcg cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa
    22741 agtgaactgg ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag
    22801 ataaccaaag acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag
    22861 atcgcgcata gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa
    22921 ccagacggta aaactcgccg ccgccactgc cggtaagacg aatttcagcg atttaaccac
    22981 ttcccgggtg atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct
    23041 gaacgatgcc gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat
    23101 ccccacttca tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac
    23161 aggcaaggtc accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag
    23221 accgttatcc cagctgggtt tcagatagct taacgggatg acaccacgtt tgatcaacat
    23281 gatggcggca ggaatcacca ccagcgcggg aatcagcgcc agacccagca acgccccttc
    23341 ataaccgccc aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat
    23401 cagcgataac gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt
    23461 gccccaggcg atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt
    23521 attaccaaac agtccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag
    23581 cgtagagaaa ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg
    23641 attatcatgg tactgggcaa cgtatttggt tacaccgtta aagatgccag ctccggcaag
    23701 cacgccgagc acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc
    23761 aaatgacaca gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact
    23821 ggccgccgtc cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag
    23881 tcgcaattac cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca
    23941 ggcgctcgct ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagcgttccc
    24001 ccgcagggca accgtgcagc ggaatgtaat ggaacaccgc catgatttcc gcttctttca
    24061 gaaagttaat caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg
    24121 cgttctgcac gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag
    24181 gcgctaacgc atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg
    24241 ctgcttccag ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc
    24301 caatatcgcg ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc
    24361 ccttttcacg gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc
    24421 cgccttcacc acccgccgta tagtttttgg tttcatggaa gctaaagcag ccaatatgac
    24481 caatggttcc cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcta
    24541 ccacaaacag attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac
    24601 ccgcgtaatg gaccggcacg ataacgcgcg ttttgtcggt gatcgccgct tcaatcagcg
    24661 tttcgtcgat gttcatggtg tccgggcgaa catccacaaa aacgattttt gcgccacgca
    24721 gcacaaaggc attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag
    24781 gctggatatc gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta
    24841 acactttggc gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc
    24901 catcgccaca cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca
    24961 ccaccggcgg tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt
    25021 tctacattcg caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc
    25081 gccacccgca aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc
    25141 tcagcacctg caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc
    25201 cgtaaagaga cataaccgcg aataccgccg gacgccgcac gtaaaatcag acattgatga
    25261 tcaaaggtgc cgcgcacggc attttcaatc cactgtgcat aaaagcgtcc gctggcgtca
    25321 ggcgcatacc acggcgcacg aaaacggctt tgcgcaaatg cggcgctggc taactgacgt
    25381 aatgcgggaa tatcggtctc ttgtgccact acagcaccac tatcactgac attgttcacg
    25441 ggtagtgcca aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc
    25501 agttcacccg tatttgatgc cgcaattttg gcctgcaccc gtgaccacgg cgctaacacg
    25561 tctggcgtca ggagctgtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag
    25621 aaggcgtttt cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag
    25681 cagatattgg ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc
    25741 atcgagccag ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg
    25801 tttttctact gtctgcacaa aggtactggc ttcaatcagg ctgtcgtgag tgccagtgtc
    25861 cagccaggca aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat
    25921 ctggttgatg gaggtaatct ccagttcacc acgctccgac ggcttcacct gctttgcgta
    25981 ctccacgact ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg
    26041 ttttggcttt tcttccagcg agatagcgcg gaaattgtcg tcaaactcca ccacgccaaa
    26101 gcgttccggg tccatcacct gatagccaaa taccgtcgcc ccttccgtgc gcgccgcaac
    26161 atgacgcagc ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca
    26221 agaaggttca ccgttgagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg
    26281 gctgggctgt tcggcgtatt ccagctggat accgaactca ctaccatcgc ccagcaggcg
    26341 ctggaaataa cctttatctt ccggcgtagt gatgatgaga atttcgcgga tatcggccaa
    26401 catcagcacc gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt
    26461 cgatacgccg cgcgtaatcg gatgcaatcg ggtgccggaa ccgcccgcca ggataatacc
    26521 tttcatgttt gcctccgctg aaaattagcc tttcagacct aaacgctcgc cctgatagct
    26581 gccgtcctgc acctgcttcc accagctttc attagccaga taccactgca ccgttttacg
    26641 cattccactt tcaaaggttt cctgtggcag ccagccaagt tcacgggcaa ttttcgaagc
    26701 atcaatggca taacgcagat catgccccgg acggtcagcg acaaaggtga tcaagtcacg
    26761 atagtgcacc acgccgtgcg gcttgttcgg agccagttct tccagcagct cgcaaatggt
    26821 ttccacaaca tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac
    26881 tttcccggtg gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc
    26941 acggatttgc tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat
    27001 catcagcggg atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt
    27061 gataagcgtc ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc
    27121 ttttgacgcg gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc
    27181 cgtcgagtgc aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga
    27241 ttttttatct tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt
    27301 cccgacaatg ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct
    27361 ttcggctgcc agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc
    27421 tgcccgatcg cagatatcaa ctttctcaaa ggcaaagcgc tcgctttgcg cgaccggtgc
    27481 cagcgacatc aggtttccgg cgtaggtcag cttatcgacc actaccaccg cgtcgctcgt
    27541 ttcgttgatg atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag
    27601 aatttttctc atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg
    27661 tcgccattga taactttaaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt
    27721 gccagcgcct cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg
    27781 tggatgttag gctcaacaac cagagtttcg ccgctatgcc actgggcgat cagttcagcg
    27841 atttccatcg ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag
    27901 caggcgattt tcagttcact ggcgcgttta tcggtagccg ccaggcaatc agccaccgcc
    27961 gctttcacct gatcgataac ccagaacggt ttgtgatcgt tcacttcgcg cgcggtacgg
    28021 ataagccgcg cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag
    28081 tgaccgccca cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg
    28141 cgaatcagtt cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca
    28201 aaagcgatat tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc
    28261 gagttagtga cgacacactc accttcgagg aaaattttgt acagttcgct ggcgcgggtc
    28321 gaacaaaccg gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc
    28381 tggcccggta acacgcgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc
    28441 tgctgtggga aagtgagatc cggacgcatc tctgctaacc attctgccat cttctcggtt
    28501 gaccccaccg gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca
    28561 atggagcgag cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc
    28621 gtgggtacag caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa
    28681 ccgccttcta cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg
    28741 ccacgattga tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc
    28801 cgtgaggcaa acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg
    28861 gtcgcaaaac tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg
    28921 cctgaccatc accatacggg ttatgggcgc ggctcatagt ttgatattcg ttttcgtctt
    28981 ttaaaagacg cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg
    29041 tacccgccgt caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc
    29101 ccagcgaagg cgcttcttcc tgaatgccgc ctgagtcggt caaaatcagc caggcgtggt
    29161 tcatcagcca gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat
    29221 gccccagaat gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa
    29281 tctggatgtc ctggtgcgtg gtggcaatgt ctgccaacgc gtggcagatt tcttcaaagc
    29341 cacgaccgaa actttcacgc ctgtgaccgg tcaccagaat catcttttta tcggggtcga
    29401 taaacgggta atttgccgcc agttctgaac gcagcgtatc gctgctcatc acctggtcac
    29461 gcacccataa cagtgcatca ataactgtat taccagtaat gaagattcgg ctatccgcaa
    29521 cgttttcacg cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca
    29581 gatgcccggt caatgtacgg ttagcctctt ccggccacgg cgaataaaga tcgcccgtgc
    29641 gcagaccagc ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg
    29701 ccagcgtcgt cgttgtgtcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa
    29761 taggttttag cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca
    29821 ttatgttgag atcgtagtca ggtacaatgg aaaagagttt cagcacctga tcgagcatct
    29881 cccgatgctg cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat
    29941 gcaccaacgg cgccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca
    30001 catcgattct cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat
    30061 ttcgagcaac ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac
    30121 ataatcatca ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc
    30181 aaatagcgat aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac
    30241 atggcccgat tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc
    30301 agtcgagcct ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg
    30361 gcaggcacat ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgt
    30421 tcaatactgt tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc
    30481 tgtgctttca tctggatggt acgcgccgcc catgcgcctt tcagctcatc attcagatgg
    30541 ctggctgcac gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc
    30601 gcggtttcag caataagctt cacgctgtca ttgaccgcgc gggtaaagtc tccggggata
    30661 aactggatgt tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc
    30721 atccgctgtt tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc
    30781 agttgcataa caaactcttt ataggcttcg tccatgaccg atggttggtc ggcagaagcc
    30841 atgtttgaac ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc
    30901 atattcaccg ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa
    30961 gtatacgcca gcgcgattaa cgcaaatgcc agccccatgc caataatcca tagcttccca
    31021 gcccacaagg tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt
    31081 ttcccaggca ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac
    31141 cacgatttct acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac
    31201 gcttaatgca atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact
    31261 ccgggacaaa atgagaatat tctgccagca cgccaatgga agcgagcagt gccgcagcaa
    31321 gggtaatcag cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa
    31381 tatgctgacg gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg
    31441 ccaccatatc cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat
    31501 gggttttgcc ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac
    31561 tgcccgcatc acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga
    31621 tgtatggcag gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt
    31681 cgaaccacaa aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc
    31741 catcaatgcc atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca
    31801 ggaagtaacc aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac
    31861 tgagataaag tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac
    31921 ggatttttac gctgatatca aaacggtcat ccagcgcgcc aatgaaaaca agcacaccgg
    31981 cacaagcgag atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc
    32041 aaatccctgc gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc
    32101 ggaagtttgg tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa
    32161 acagtgtcgt gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa
    32221 tgcatgctct cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag
    32281 ggaaagtccg ttccgaaccc accagtcctg tgcaaaaatt aaccatttga tatgcaaatt
    32341 gcataacacg ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt
    32401 acagatataa aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta
    32461 tgagcgtttc atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat
    32521 gaggaattcc attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat
    32581 tttctgcagt tcttcctgag tagtgagcag ttcttcttta cgggtaccgg aacggttgta
    32641 gtcgatagcc gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt
    32701 gcctgtacct ttaaactctt cgtagataac ttcgtccatt ttagaaccgg tatcgataag
    32761 cgccgtcgcg ataatagtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa
    32821 gcgtttcgga cgatgcaggg cgttggcatc cacaccaccg gtcaacactt tacctgacgc
    32881 cggaacaacg gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac
    32941 gtctttcttg tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac
    33001 gtggcgagat gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg
    33061 catctcggtt acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg
    33121 gtggttgtaa gcaatgctct gggcaatgtt ctgcagcagc atggttttac cggctttcgg
    33181 cggtgccaca atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg
    33241 agcagttaaa tcttcagtag aaccgttacc acgttccata cgcagacgag aatttgcgtg
    33301 cagcggggtt aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt
    33361 aacttcgtta actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt
    33421 accagagatg gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta
    33481 gatgtcatca ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc
    33541 ctgcaatatc tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt
    33601 caggatggca aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt
    33661 ttcgccgaga gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt
    33721 ggtgtgagtt cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg
    33781 atcatccatg cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga
    33841 gtacgcagaa ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg
    33901 catttaagat aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt
    33961 taatacgaag tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg
    34021 gggcacccga acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga
    34081 cctttagaca gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga
    34141 gtcgggatac cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt
    34201 tttgcaacgg tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc
    34261 attttgcacg gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg
    34321 agtacatccg tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac
    34381 tccacaggaa taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag
    34441 gttgacttta tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac
    34501 actatgagca aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag
    34561 gttgtagaag cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc
    34621 cttccgctga cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa
    34681 acgatggcgt ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat
    34741 cgcaaggtga atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag
    34801 atccatgccg acgcagaacc gctggcggaa gctactggcc tgaagctggg tctggcttac
    34861 ggtggtgatg gctacgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt
    34921 ggcaccacgg gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt
    34981 caggtagtgg ttctggatga agccgatcgc atgtacgatc tgggctttat taaagatatc
    35041 cgctggctgt tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc
    35101 acgctttcgt accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt
    35161 gaagtggaac cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct
    35221 aacgaagaaa aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg
    35281 attattttcg ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat
    35341 ggtcatcgtg tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt
    35401 gatgaattta cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt
    35461 ttgcatattc cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat
    35521 tacgttcacc gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg
    35581 gcgtgtgaag agtatgcatt gaatttgcct gctattgaaa cctatattgg tcactcaatt
    35641 ccggtaagca aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc
    35701 acgcgcccac gtacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt
    35761 tcaggttaaa taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa
    35821 tgaatcccga tgagcatact gtagtaagtg attcaggtga aagaatgcag ccaacacaga
    35881 gacagattga aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct
    35941 cggttcgaat agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct
    36001 gacgcgaata aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc
    36061 taatgaagca atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga
    36121 tatccctccc tcgcaaattc gcgttgtcgc tacggcgaca ttacgtcttg ccgtcaatgc
    36181 gggtgatttt attgccaatg cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg
    36241 tgaagaggaa gcacgtctga tttatcaggg cgttgctcac acaactggcg gtgccgatca
    36301 gcgcctggtg gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca
    36361 aaccacctcg ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc
    36421 cgatcgtaat ctggggcagg aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt
    36481 acgtccggtt gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg
    36541 caccgtgcag gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct
    36601 ggaaaagttg cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga
    36661 gattgacggg ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat
    36721 cgccattttt accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga
    36781 aggcctggtc tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacgct
    36841 gcgtaatatt cagcgccgct ttatgatcga tattgatcag gcgcagcgcg tagccaaagt
    36901 tgcggctaac ttcttcgatc aggtggaaaa ggaatggcat cttgaagcaa taagccgcga
    36961 tttgctcatc agcgcctgtc agcttcatga aatcggcctg agcgttgact ttaaacaagc
    37021 gccgcaacac gctgcttatc tggtgcgtaa tctggatctt cccggtttta cccccgcaca
    37081 gaaaaaattg ctggcgacgc tactgctcaa ccagactaat ccggtcgatc tctcatcgct
    37141 gcatcagcaa aatgccgtac cgccgcgcgt cgcagaacaa ctctgccgtt tactccgcct
    37201 ggccatcatt tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc
    37261 taaccatgaa ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg
    37321 taaagagatt attgctcagg aaagccagtg gcagagctat gtccactggc cgctggaagt
    37381 gcattaatga gtaagtgccg gatgcgatgc tgacgcatct tttccggcct tgattatttc
    37441 cctcgttttg ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc
    37501 cgttcttcgg cgctgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc
    37561 tccagcaaaa agcggctcgg ctccgggcgc accagctcgc cgtactgacg gcgttcttta
    37621 cacagcgtaa aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc
    37681 tcctcatcga tattatcttc atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata
    37741 ccgaccatgt agacataagg aaactccagc ccttttgacg cgtgcagagt catcagttgc
    37801 acctgatcca gctcttcttc actctcacca cgctccatca tgtcgcgcaa agtaaagcgc
    37861 gtcaccacct gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc
    37921 atccagctaa acagttggtt gacgttcttc atgcgcattt cggcggcttt cgggctgggc
    37981 gatgtttcgt acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca
    38041 atcggctccc gctccgccag acgctggatt tctgccaacc agtgagtaaa gcgggtcaat
    38101 gcttcataac cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac
    38161 atgcttttat tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc
    38221 tctctcttcg gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc
    38281 acgcgcagat aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca
    38341 gaaattttgt acgggatgcg gttttgcatc aggaattttt caaacactcg cgactgatgg
    38401 ttaccgcgat aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca
    38461 atcagttcac cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaacttta
    38521 agctctgtgc cgtaacccag ttcggagaac agacgcttct caaagacgtg cgggttattg
    38581 gcgatcagga tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc
    38641 ttaatcacct tcagcgccgg aaaatcctga ctcagcagca ccaggttttg tggacgtgcg
    38701 ccgcgccagg agtagatcga ctggtcgtca tcgcccacca cggtaaagcg cgcgcggctg
    38761 cccaccagca gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ctcatccacc
    38821 agcagatagc gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagcagc
    38881 aacgtcggca ataaaatcag atcatcgaag tcgagaacat tgcacgcttt caggtgtgca
    38941 tcatacagcc cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc
    39001 gcctgggacg gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc
    39061 aggagaactt tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga
    39121 tcggtatcgt caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg
    39181 atgatatcca gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga
    39241 cccagcgtct gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc
    39301 gccgcgatat gtcgcgcctg ataaccgcaa ccgcggatca gatgggcgat tttattggtg
    39361 atcacacgag ttttaccgga acccgcgccc gccagcacca ggcaggggcc ggtaacgaat
    39421 tcgacagctt gttgttggcc ggggtttaga cgcataggtg tattgctcaa tcttcgaacg
    39481 gggggaggat tgtagcagaa tcgcatttga atgtgaccgc gtgtaagccg ctaaccgatt
    39541 ggctgcggat gtttaaatca gagagggaag accgaaaccg gagttcatcg tgaatccatt
    39601 agacttagaa aatatcgggt cgtaacgttg tgttattact ttttcagagt ttactaagga
    39661 gaggtgtact cgtggcgatt aatttcagtc ctaaggttgg tgaaatactg gattgcaact
    39721 ttggtaacta tcctgttagc cagaatggac agttcagcac cacgtattac gatggccgca
    39781 ttcctcctga aatgataaaa aacaggttag t
//
LOCUS       DAJTXQ010000021        39566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000021 DAJTXQ010000000
VERSION     DAJTXQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.021"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..164)
                     /locus_tag="OO515_001072"
     CDS             complement(<1..164)
                     /locus_tag="OO515_001072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575059.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGA"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="OO515_001073"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="OO515_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6575060.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="OO515_001074"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="OO515_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6575061.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="OO515_001075"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="OO515_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6575062.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="OO515_001076"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="OO515_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6575063.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="OO515_001077"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="OO515_001077"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6575064.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="OO515_001078"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="OO515_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6575065.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="OO515_001079"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="OO515_001079"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6575066.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="OO515_001080"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="OO515_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6575067.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="OO515_001081"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="OO515_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6575068.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="OO515_001082"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="OO515_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6575069.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="OO515_001083"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="OO515_001083"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6575070.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="OO515_001084"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="OO515_001084"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6575071.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="OO515_001085"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="OO515_001085"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6575072.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="OO515_001086"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="OO515_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6575073.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="OO515_001087"
     CDS             complement(14282..14581)
                     /locus_tag="OO515_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6575074.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="OO515_001088"
     CDS             complement(14793..15233)
                     /locus_tag="OO515_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6575075.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="OO515_001089"
     CDS             15334..15933
                     /locus_tag="OO515_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6575076.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="OO515_001090"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="OO515_001090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6575077.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="OO515_001091"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="OO515_001091"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6575078.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="OO515_001092"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="OO515_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6575079.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="OO515_001093"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="OO515_001093"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6575080.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="OO515_001094"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="OO515_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6575081.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="OO515_001095"
     CDS             complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="OO515_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6575082.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21877..22575
                     /gene="cpxR"
                     /locus_tag="OO515_001096"
     CDS             21877..22575
                     /gene="cpxR"
                     /locus_tag="OO515_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6575083.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22572..23945
                     /gene="cpxA"
                     /locus_tag="OO515_001097"
     CDS             22572..23945
                     /gene="cpxA"
                     /locus_tag="OO515_001097"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6575084.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="OO515_001098"
     CDS             complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="OO515_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6575085.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="OO515_001099"
     CDS             complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="OO515_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6575086.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="OO515_001100"
     CDS             complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="OO515_001100"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6575087.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27022..28056
                     /gene="rhaT"
                     /locus_tag="OO515_001101"
     CDS             27022..28056
                     /gene="rhaT"
                     /locus_tag="OO515_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6575088.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGAGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="OO515_001102"
                     /pseudo
     CDS             complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="OO515_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="OO515_001103"
     CDS             complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="OO515_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6575089.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30099..31568
                     /gene="rhaB"
                     /locus_tag="OO515_001104"
     CDS             30099..31568
                     /gene="rhaB"
                     /locus_tag="OO515_001104"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6575090.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31565..32824
                     /gene="rhaA"
                     /locus_tag="OO515_001105"
     CDS             31565..32824
                     /gene="rhaA"
                     /locus_tag="OO515_001105"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6575091.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33066..33890
                     /gene="rhaD"
                     /locus_tag="OO515_001106"
     CDS             33066..33890
                     /gene="rhaD"
                     /locus_tag="OO515_001106"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6575092.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33900..34214
                     /gene="rhaM"
                     /locus_tag="OO515_001107"
     CDS             33900..34214
                     /gene="rhaM"
                     /locus_tag="OO515_001107"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6575093.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34515..34961
                     /locus_tag="OO515_001108"
     CDS             34515..34961
                     /locus_tag="OO515_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6575094.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34972..36423
                     /locus_tag="OO515_001109"
     CDS             34972..36423
                     /locus_tag="OO515_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6575095.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36413..37483
                     /locus_tag="OO515_001110"
     CDS             36413..37483
                     /locus_tag="OO515_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6575096.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37483..39231
                     /locus_tag="OO515_001111"
     CDS             37483..39231
                     /locus_tag="OO515_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6575097.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39280..>39566)
                     /locus_tag="OO515_001112"
     CDS             complement(39280..>39566)
                     /locus_tag="OO515_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6575098.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9615 a        10281 c        10274 g         9396 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacagcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgccca
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agctaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccacgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactccca ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg
    21181 tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg
    21241 agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca
    21301 actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca
    21361 ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg
    21421 ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc
    21481 gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca
    21541 taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc
    21601 tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt
    21661 cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta
    21721 tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt
    21781 caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc
    21841 tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat
    21901 gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg
    21961 attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt
    22021 ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag
    22081 acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc
    22141 ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg
    22201 gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca aaacaacgac
    22261 aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg tcaggaagcc
    22321 agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg
    22381 gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa
    22441 cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg tcgtaaactg
    22501 ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg
    22561 gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc
    22621 tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg
    22681 agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc gaagcggagc
    22741 tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata
    22801 agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg
    22861 ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc
    22921 atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg
    22981 gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca
    23041 ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg gtcagtacgc
    23101 cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg
    23161 ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg
    23221 aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga
    23281 cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc
    23341 tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctcgagcgta
    23401 ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta
    23461 atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg tggagtgaag
    23521 tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc
    23581 cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca
    23641 ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata
    23701 aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg
    23761 aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa tctggcggta
    23821 caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg
    23881 cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga
    23941 gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag
    24001 gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc
    24061 cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc
    24121 gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc gtcatcaaac
    24181 ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc
    24241 aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata caaccacccc
    24301 accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt
    24361 ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca gcggaaaata
    24421 tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc gccaaacgca
    24481 ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag atagtgctca
    24541 cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg
    24601 tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat tttggcaatt
    24661 gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat
    24721 cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg
    24781 cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg acgaacgtta
    24841 gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta aaatttcgat
    24901 tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat
    24961 cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca tcggtttaaa
    25021 cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga
    25081 agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg
    25141 aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac cagtctgggc
    25201 aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa tcagcgtttg
    25261 cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg caaagcccac
    25321 caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt
    25381 gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc
    25441 agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat
    25501 atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa cttcaacacc
    25561 atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt
    25621 taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc
    25681 ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt
    25741 gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa
    25801 tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc
    25861 catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga gcatcagtgt
    25921 tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac cacatcaatt
    25981 gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa
    26041 ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac gcctcattgc
    26101 agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg tcccagttca
    26161 ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc aggtagtaag
    26221 catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa atagcttcac
    26281 ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg cctttcagca
    26341 ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga
    26401 agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc tgcagggtgg
    26461 tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc
    26521 gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg
    26581 gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct
    26641 gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat
    26701 cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt attgtcgggc
    26761 ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa
    26821 aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat
    26881 gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg gcggcaacag
    26941 gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg tgaagcgacg
    27001 aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt ttggcatttg
    27061 atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa aaaatggtca
    27121 tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc gtggaccatc
    27181 agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct ctctacgctg
    27241 ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta tggcctgacc
    27301 atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac gttgattgtc
    27361 ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa caccgaaggc
    27421 ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgcagggat tgtaactcga
    27481 gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa tctgaaaaaa
    27541 gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac
    27601 gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact gtatgtcgct
    27661 ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg tttctgtttt
    27721 attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg
    27781 ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta tctgcaattc
    27841 tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat cagttggatg
    27901 ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg
    27961 aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc
    28021 gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt gagatgacgc
    28081 cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata gttactatct
    28141 tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg gagaagatat
    28201 tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc agtctgctgg
    28261 cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa tttatccagc
    28321 gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag caacgtttcg
    28381 ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa catcaccaac
    28441 tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact actttcatgc
    28501 tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt
    28561 ggttgccctg cgctggcgct aaatcccgga atcgctccct gtcagtcaag attcagcttc
    28621 agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga agcgtaggag
    28681 tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata aggacgatcg
    28741 ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa atcatgtgta
    28801 tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg ctggtcgctg
    28861 gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac ctcggccaga
    28921 gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt cgcgttattg
    28981 cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa
    29041 aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt cagtaacgct
    29101 ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg
    29161 ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga
    29221 aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct ccagccaggc
    29281 cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg
    29341 cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt
    29401 ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca atacgctgtg
    29461 gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca actgattcag
    29521 cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca gacacagatt
    29581 atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg tgccaccggt
    29641 gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga caatcacaat
    29701 ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg gttctatcgc
    29761 cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca tactggcctc
    29821 ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc ttaaattttc
    29881 gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat
    29941 gcggtgagca tcacatcacc acaatgaagc taattgtgaa catcatcacg ttcatctttc
    30001 cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt
    30061 tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc aattgtgtcg
    30121 ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc
    30181 gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc cagaacggtt
    30241 atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta aacaaggtgt
    30301 gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg gactttgtgc
    30361 tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat agccgctcca
    30421 atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat caacgtagcg
    30481 gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg gagcaacaac
    30541 ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc agctatcgcc
    30601 tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg gtcaatatca
    30661 atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa gcctggtttg
    30721 gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag ggtaatgaga
    30781 ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc tcgccgttaa
    30841 acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc ttcgaaagcc
    30901 agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa ggcggggcgg
    30961 aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag cgagtgcttc
    31021 aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca cttccggcct
    31081 gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg atgtgcagcg
    31141 aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat gctgaactgg
    31201 cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc
    31261 agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc cagaacacgc
    31321 tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg cctgttgaag
    31381 cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc aacaatgtgg
    31441 atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc cctaatcctg
    31501 acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca aaggagcttt
    31561 gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt
    31621 ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt caatgcactg
    31681 ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga ccggtgggat
    31741 tcaggctatt ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac gagccgatct
    31801 ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg ccatctatct
    31861 ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca aaaactgggt
    31921 tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt tctcgcatcc
    31981 gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc agttctggat
    32041 tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc
    32101 atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg accgtctcgc
    32161 cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc tggatccggc
    32221 gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga gctacacggt
    32281 tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc tgtgcctgga
    32341 cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg ccatgctgta
    32401 tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg atcacgtggt
    32461 gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg atctgttcga
    32521 ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg ccgcgtgggt
    32581 cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga
    32641 gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac tggaagaaca
    32701 gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg atacgccagc
    32761 aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg
    32821 gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga atgaccatgc
    32881 gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa acatagcctc
    32941 gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg gggagagggt
    33001 tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt acaggaacac
    33061 agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg
    33121 atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg
    33181 acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat attcccctca
    33241 gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct
    33301 tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg
    33361 gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact tccgaacttc
    33421 cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga
    33481 tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg
    33541 cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg
    33601 gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac
    33661 aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt
    33721 cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag
    33781 tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc
    33841 tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa ggagcaaaca
    33901 tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc
    33961 ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact
    34021 acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg
    34081 aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg aaatatatga
    34141 ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg caagaagtgt
    34201 tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat tttaaatttc
    34261 gatttgaaaa attaaacata ttacatctcc cggtaagtta tatttccctg atacattgtg
    34321 agtaaatcac aaaaataatg aataacccat taatgattca tgtggtttat ttaaataacc
    34381 attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt cattgtttac
    34441 cgacatatcg cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt
    34501 atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat attcagggca
    34561 atggcgctta ttccgttctg aagcagttgg cgacaatagc gttacaaaac ggttttatca
    34621 ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat
    34681 ttggttccgg tgtcgccgtg ccgcacggta aaagcgcctg cgttaaacaa ccgtttgtat
    34741 tattcgcccg caaagcgcag gctattgact ggaaagccag cgatggcgaa gacgtcaatt
    34801 gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa atcatcggca
    34861 cactgtgtcg caaaattatc caccaggatt ttattcatca actgcaacag ggcgacgccg
    34921 accaggtgct tgccttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca
    34981 tccttatgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg
    35041 gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa
    35101 gggtccagtg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc
    35161 attctcgcta ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc cgggaagaaa
    35221 gtttatgaga ttgccatctc ccaggcgttg aaaaatatcg accagatttt cagcgaatta
    35281 ccgacaaact cgcagctttt tgccgcagat agcggcgtga agcttggcaa acaggaagtg
    35341 caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccgtttgtc
    35401 atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccgtat
    35461 accgatatgt cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat cggttacctt
    35521 ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa
    35581 ccggctttcg ctccggcgtt tctggtttgc tatctggcga acgacaaagc gctgctcggc
    35641 acccagtcgg gtgcgggatt cctcggcgcg gtggtgctgg ggctggcgat tggctatttc
    35701 gtcttctggt tccgcaaagt gcgccttggc aaagcattac aaccgctgct cggttcaatg
    35761 ctgatcccgt tcgttactct gctggttttc ggcgtgctca cttactacgt tatcgggccg
    35821 gtgatgtccg acctcatggg cgggctgctc cacttcctga ataccattcc tccatcaatg
    35881 aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg cggccccatc
    35941 aataaaaccg cctggttctt ctgcttctca ctgctggaaa aacacattta tgactggtac
    36001 gccatcgtcg gcgttgtcgc gttgatgccg cccgttgcgg cgggtcttgc gacgttcatt
    36061 gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca gtagtgccat tgtggtcggt
    36121 gccacagtcg caaccgaacc ggctatacct tacgctctgg cagctccgtt gccgatgatt
    36181 accgccaata cgctggcggg tggcattacc ggcgtgctgg tgatcgcttt cggaatcaaa
    36241 cgtctggcac ctggtttggg tatctttgac ccgctgatcg gcctgatgtc gccggtgggt
    36301 tcgttttatc tggtgctagc gattggcctc gcgctgaaca tttcattcat tatcgtcctg
    36361 aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt ccatgaacat
    36421 tgagttactg caacagttgt gcgaagccag cgccgtcagc ggcgatgaac aggaagttcg
    36481 cgacattctg ataaacacgc tggaaccttg cgttaatgag atcacctttg atggtctggg
    36541 cagctttgtt gcccgtaagg ggaataaagg tccaaaagtt gccgttgtca ggcatatgga
    36601 tgaagtcggc tttatggtca cccacatcga cgagagcggt tttctgcgct ttaccaccat
    36661 tggcggctgg tggaatcagt caatgctcaa ccaccgggta accatacgca cacacaaggg
    36721 atttaaaatc cctggtgtga ttggttccgt cgcacctcat gcgttaacgg aaaagcaaaa
    36781 gcaacagccg ctgtcatttg atgagatgtt cattgatatt ggcgcgaaca gtcgcgatga
    36841 ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc ccggaagcca attttgcctg
    36901 ctggggcgaa gataaagtgg tcggcaaggc gctggataac cgcatcggct gcgcaatgat
    36961 ggctgaacta ttgcagacgg tgaataatcc cgaaattacg ctgtatggcg tcggcagcgt
    37021 ggaagaagaa gttgggctac gcggggcaca aacctcggcg gaacacatta aaccggatgt
    37081 ggtgattgtg ctggataccg ctgtcgcagg tgatgttccg ggcattgata acattaaata
    37141 cccgctgaaa ctgggccagg ggccggggct gatgctgttt gacaagcgct acttccccaa
    37201 ccagaaactg gtggcggcgt taaaaagctg tgccgcacat aacgatttac cgctgcaatt
    37261 ttccaccatg aaaaccggtg cgacggatgg cgggcgctac aacgtgatgg gcggcgggcg
    37321 tccggttgtt gcgctgtgcc tgccaacccg ctatctgcac gctaacagcg gcatgatttc
    37381 aaaagccgat tacgatgctc tgctcacgtt gatacgggat tttctgacca ccttaaccgc
    37441 ggagaaagtc aacgcgttta gccagttccg tcaggtggat taatgctaaa cgaacgccag
    37501 ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc ctggtgagct ggcgcaacag
    37561 actggcgttt caggcaggac catcctgcgt gatattgact atctcaactt tacccttaac
    37621 ggcaaagccc gcattttcgc cagtggcagt gcgggctatc agctggaaat cttcgagcgc
    37681 cgcagctttt ttcagttgct gcaaaagcac gataacgacg atcggctgct ggcgctgtta
    37741 ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg cgcttaattt gccagaaacc
    37801 tgggtagcag agcgtctgcc ccggttaaaa cagcgttatg aacgcacttg ttgcctggcc
    37861 agtcgccccg gtttgggcca tttcattgat gagacagaag agaaacgcgt tatcttgctg
    37921 gcgaacttgc tgcgcaaaga tccgttttta attccgctgg cgggcgtaac acgagacaat
    37981 tttcagcatt tatccacggc ctgcgacaac caacaccgct ggccgctcat gcagggtgat
    38041 tatctctcca gcctgattct ggcgatttac gccctgcgta atcaactgac cgatgagtgg
    38101 ccgcaatatc ccggtaacga gataaaacaa atcgttgaac agagcggcat gtttcttggt
    38161 gataacgctg taagaaccct gacaggtttg atagagaaac agcatcagca agcgcaggta
    38221 atttcagccg atcatgtgct ggggttgctg caaagggtgc caggcatcgc gtcattgaat
    38281 attattgata cgcagctggt tgagaatatt accgggcatt tattacgttg ccttgccgca
    38341 ccggtgtgga ttgccgagca ccgccaaagc agcatgaata acctgaaagc cgcctggcct
    38401 gcggcctttg atatgagtct gcactttatt acgctactgc gtgaacagct cgatattccc
    38461 cttttcgata gcgatctgat cggtttgtat tttgcctgtg cgctggaacg gcatcaaaac
    38521 gaacgccagc cgatcatttt gctctcggac cagaacgcga ttgccactat taatcagctc
    38581 gccattgagc gcgatgtttt acattgtcgg gtgattattg cccgtagctt aagcgagctt
    38641 attgccattc gcgaagagat tgagccgtta ttgatcatta acaacagcca ttatttactg
    38701 gatgacgcgg taaataatta catcaccgta aaaaatatca ttacggctgc cgatatcgaa
    38761 caaataaaac actttctggc gacggcattt attcgccaac agccggaacg ctttttctct
    38821 gcccccggaa gttttcatta ttcgaatgta cgcggtgaaa gctggcaaca tattacccgg
    38881 caaatttgtg cgcaattagt ggcacaacac catattaccg ccgatgaagc gcaacggatc
    38941 atcgcccgcg aaggcgaagg tgaaaacctg attgttaatc gcctcgcaat cccacattgc
    39001 tggagcgaac aggagcgacg ttttcgtgga ttttttatta ccctcgccca accagttgag
    39061 gtgaataacg aagtcattaa ccatgtcttg atcgcctgcg ccgccgccga tgcgcgtcat
    39121 gagctaaaaa tatttagcta tctggcaagc gtattgtgtc agcatccggc agaagttatt
    39181 gccgggttaa caggatatga ggcatttatg gagttacttc acaaggggtg aactgtgtcg
    39241 gatgcgccgc atccgacact gcattttcat caaaaacgtt taacgcaggc tattatagtc
    39301 atcaaccatt tcgttgtact cgcgtaacgc tcgtggaaat gagtcttcga ccatccagct
    39361 tgtgtttgca tcctgaagat gctctttatc ccagtcagag tacgggactt tatctctgac
    39421 gcggcgaaca tattttttag tctcgagttg atactggtct gccgaattaa tgaacgagaa
    39481 attcataccg cctttatccg cttctttggc ctgcgctacc agcgtttcca gttcagagac
    39541 tttcttcatt gccgcatcaa catcaa
//
LOCUS       DAJTXQ010000022        39256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000022 DAJTXQ010000000
VERSION     DAJTXQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.022"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO515_001113"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO515_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6575099.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO515_001114"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO515_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6575100.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OO515_001115"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OO515_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6575101.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OO515_001116"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OO515_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6575102.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OO515_001117"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OO515_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6575103.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OO515_001118"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OO515_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6575104.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OO515_001119"
     CDS             complement(6574..7338)
                     /locus_tag="OO515_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6575105.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO515_001120"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO515_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6575106.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OO515_001121"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OO515_001121"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6575107.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OO515_001122"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OO515_001122"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6575108.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OO515_001123"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OO515_001123"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6575109.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OO515_001124"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OO515_001124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6575110.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OO515_001125"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OO515_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6575111.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OO515_001126"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OO515_001126"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6575112.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO515_001127"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO515_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6575113.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OO515_001128"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OO515_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6575114.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OO515_001129"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OO515_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6575115.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OO515_001130"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OO515_001130"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6575116.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OO515_001131"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OO515_001131"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6575117.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OO515_001132"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OO515_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6575118.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OO515_001133"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OO515_001133"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6575119.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO515_001134"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO515_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6575120.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO515_001135"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO515_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6575121.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO515_001136"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO515_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6575122.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OO515_001137"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OO515_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO515_001138"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO515_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6575123.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OO515_001139"
     CDS             complement(31717..33003)
                     /locus_tag="OO515_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6575124.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OO515_001140"
     CDS             complement(33054..33995)
                     /locus_tag="OO515_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6575125.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OO515_001141"
     CDS             complement(34010..34780)
                     /locus_tag="OO515_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6575126.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35254..36768
                     /gene="caiT"
                     /locus_tag="OO515_001142"
     CDS             35254..36768
                     /gene="caiT"
                     /locus_tag="OO515_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6575127.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36799..37941
                     /gene="caiA"
                     /locus_tag="OO515_001143"
     CDS             36799..37941
                     /gene="caiA"
                     /locus_tag="OO515_001143"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6575128.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38070..39256
                     /gene="caiB"
                     /locus_tag="OO515_001144"
                     /pseudo
     CDS             38070..39256
                     /gene="caiB"
                     /locus_tag="OO515_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9394 a        10790 c        10280 g         8792 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaaatg
    35101 tgataacaat cacagaatgc agcttattga atacccatta tgagttagcc attaacgcgt
    35161 ccacgaggtt aataataatt atatcaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    35221 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    35281 atagaaccga aggttttctt tccgccgtta ataatcgtcg gcatactttg ttggcttaca
    35341 gtcagagatc tggatgcagc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    35401 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    35461 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cacctgaatt tagtaccgcc
    35521 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    35581 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacaggg
    35641 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    35701 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    35761 cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggc
    35821 actatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    35881 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    35941 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    36001 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    36061 ctgggttggg tgttcattgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    36121 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    36181 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttatgctatc
    36241 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    36301 ggcatggtgc tggggctgac agcgtcaacc tggatcctgt ggactgtact cggtagtaac
    36361 actctgctgt tgatagataa aaacatcatc aacattccaa atctgatcga acagtacggt
    36421 gtggcgcgcg ccatcattga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    36481 ggcttcttca tcctctgctt tattgccacc gttacactgg ttaacgcctg ctcttatacc
    36541 ctggcgatgt ccacttgccg cgaagtacgc gatggtgaag aaccacctct gctggtgcgt
    36601 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    36661 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    36721 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    36781 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    36841 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    36901 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    36961 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    37021 atggagctgg gacgtctggg ggcaccaacc tatgtgctgt accagttgcc gggcgggttc
    37081 aacaccttcc tgcgcgaagg cacacaagag cagatcgaca aaattatggc tttccgcggc
    37141 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    37201 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    37261 tttattacca gcagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    37321 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    37381 accaaacttg aaaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgac
    37441 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    37501 gaagagttcg accatgaacg tttcctggta gccctcacca actacggtac ggcgatgtgc
    37561 gcttttgaag atgcggcgcg ctacgccaat cagcgcgtgc agtttggcga ggctattggt
    37621 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    37681 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    37741 gcgatgtgca aatacttctg cgccaatgcg gcatttgaag ttgtggatag cgcaatgcag
    37801 gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgatctg
    37861 cgtgtagacc gcgtctccgg gggatctgac gaaatgcaga tcctgacgct gggtcgcgcg
    37921 gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctaat gcgtcttatc
    37981 aggcctacgg gagcacgaat gtaggccgga taaagcgttt acgccgcatc cggcagtcat
    38041 gcactacata aatttttcag gagctaataa tggatcatct acccatgccg aaattcgggc
    38101 cgttggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccgggccg ttcgccgggc
    38161 aaatgttcgc agaatggggt gcggaagtta tctggatcga gaacgtcgcc taggccgaca
    38221 ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt
    38281 taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata
    38341 tcttcatcga agccagcaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac
    38401 tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca
    38461 ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc
    38521 tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact
    38581 tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg
    38641 gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact
    38701 tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc
    38761 cctactacgc cggttgcggt ctgtataaat gcgccgacgg ctacatcgtg atggaactgg
    38821 tgggcattac ccaaattgaa gagtgcttta aagatattga cctcgcacat ctactcggta
    38881 cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac
    38941 tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac
    39001 gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca
    39061 atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct
    39121 gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa
    39181 tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg
    39241 acattcagga gttggt
//
LOCUS       DAJTXQ010000023        39204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000023 DAJTXQ010000000
VERSION     DAJTXQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.023"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..108)
                     /locus_tag="OO515_001145"
     tRNA            complement(33..108)
                     /locus_tag="OO515_001145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Lys,seq:ttt)
     gene            complement(157..232)
                     /locus_tag="OO515_001146"
     tRNA            complement(157..232)
                     /locus_tag="OO515_001146"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Lys,seq:ttt)
     gene            complement(236..311)
                     /locus_tag="OO515_001147"
     tRNA            complement(236..311)
                     /locus_tag="OO515_001147"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Val,seq:tac)
     gene            complement(461..536)
                     /locus_tag="OO515_001148"
     tRNA            complement(461..536)
                     /locus_tag="OO515_001148"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Lys,seq:ttt)
     gene            complement(701..1492)
                     /gene="cpoB"
                     /locus_tag="OO515_001149"
     CDS             complement(701..1492)
                     /gene="cpoB"
                     /locus_tag="OO515_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6575129.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1502..2023)
                     /gene="pal"
                     /locus_tag="OO515_001150"
     CDS             complement(1502..2023)
                     /gene="pal"
                     /locus_tag="OO515_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6575130.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2058..3350)
                     /gene="tolB"
                     /locus_tag="OO515_001151"
     CDS             complement(2058..3350)
                     /gene="tolB"
                     /locus_tag="OO515_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6575131.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3483..4715)
                     /gene="tolA"
                     /locus_tag="OO515_001152"
     CDS             complement(3483..4715)
                     /gene="tolA"
                     /locus_tag="OO515_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6575132.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4780..5208)
                     /gene="tolR"
                     /locus_tag="OO515_001153"
     CDS             complement(4780..5208)
                     /gene="tolR"
                     /locus_tag="OO515_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6575133.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5212..5904)
                     /gene="tolQ"
                     /locus_tag="OO515_001154"
     CDS             complement(5212..5904)
                     /gene="tolQ"
                     /locus_tag="OO515_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6575134.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5901..6305)
                     /gene="ybgC"
                     /locus_tag="OO515_001155"
     CDS             complement(5901..6305)
                     /gene="ybgC"
                     /locus_tag="OO515_001155"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6575135.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYVPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6455..6748)
                     /gene="ybgE"
                     /locus_tag="OO515_001156"
     CDS             complement(6455..6748)
                     /gene="ybgE"
                     /locus_tag="OO515_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6575136.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6748..6861)
                     /gene="cydX"
                     /locus_tag="OO515_001157"
     CDS             complement(6748..6861)
                     /gene="cydX"
                     /locus_tag="OO515_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6575137.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6876..8015)
                     /gene="cydB"
                     /locus_tag="OO515_001158"
     CDS             complement(6876..8015)
                     /gene="cydB"
                     /locus_tag="OO515_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6575138.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8031..9599)
                     /gene="cydA"
                     /locus_tag="OO515_001159"
     CDS             complement(8031..9599)
                     /gene="cydA"
                     /locus_tag="OO515_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6575139.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10195..10422)
                     /locus_tag="OO515_001160"
     CDS             complement(10195..10422)
                     /locus_tag="OO515_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575140.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10447..13003)
                     /locus_tag="OO515_001161"
                     /pseudo
     CDS             complement(10447..13003)
                     /locus_tag="OO515_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(13021..14937)
                     /gene="mngA"
                     /locus_tag="OO515_001162"
     CDS             complement(13021..14937)
                     /gene="mngA"
                     /locus_tag="OO515_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6575141.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15106..15828
                     /gene="mngR"
                     /locus_tag="OO515_001163"
     CDS             15106..15828
                     /gene="mngR"
                     /locus_tag="OO515_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6575142.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15932..16801)
                     /gene="sucD"
                     /locus_tag="OO515_001164"
     CDS             complement(15932..16801)
                     /gene="sucD"
                     /locus_tag="OO515_001164"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6575143.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16801..17967)
                     /gene="sucC"
                     /locus_tag="OO515_001165"
     CDS             complement(16801..17967)
                     /gene="sucC"
                     /locus_tag="OO515_001165"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6575144.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18061..19278)
                     /gene="odhB"
                     /locus_tag="OO515_001166"
     CDS             complement(18061..19278)
                     /gene="odhB"
                     /locus_tag="OO515_001166"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6575145.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19293..22094)
                     /gene="sucA"
                     /locus_tag="OO515_001167"
     CDS             complement(19293..22094)
                     /gene="sucA"
                     /locus_tag="OO515_001167"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6575146.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22395..23111)
                     /gene="sdhB"
                     /locus_tag="OO515_001168"
     CDS             complement(22395..23111)
                     /gene="sdhB"
                     /locus_tag="OO515_001168"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6575147.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23127..24893)
                     /gene="sdhA"
                     /locus_tag="OO515_001169"
     CDS             complement(23127..24893)
                     /gene="sdhA"
                     /locus_tag="OO515_001169"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6575148.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24893..25240)
                     /gene="sdhD"
                     /locus_tag="OO515_001170"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24893..25240)
                     /gene="sdhD"
                     /locus_tag="OO515_001170"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6575149.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25234..25638)
                     /gene="sdhC"
                     /locus_tag="OO515_001171"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25234..25638)
                     /gene="sdhC"
                     /locus_tag="OO515_001171"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6575150.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26019..26126
                     /locus_tag="OO515_001172"
     CDS             26019..26126
                     /locus_tag="OO515_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575151.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26332..27615
                     /gene="gltA"
                     /locus_tag="OO515_001173"
     CDS             26332..27615
                     /gene="gltA"
                     /locus_tag="OO515_001173"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6575152.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28005..28571
                     /locus_tag="OO515_001174"
     CDS             28005..28571
                     /locus_tag="OO515_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6575153.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28622..31080
                     /locus_tag="OO515_001175"
                     /pseudo
     CDS             28622..31080
                     /locus_tag="OO515_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31095..31826
                     /locus_tag="OO515_001176"
     CDS             31095..31826
                     /locus_tag="OO515_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6575154.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31823..32884
                     /locus_tag="OO515_001177"
     CDS             31823..32884
                     /locus_tag="OO515_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575155.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33036..34082
                     /locus_tag="OO515_001178"
     CDS             33036..34082
                     /locus_tag="OO515_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6575156.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34079..34870)
                     /gene="nei"
                     /locus_tag="OO515_001179"
     CDS             complement(34079..34870)
                     /gene="nei"
                     /locus_tag="OO515_001179"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6575157.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34906..35640)
                     /gene="pxpA"
                     /locus_tag="OO515_001180"
     CDS             complement(34906..35640)
                     /gene="pxpA"
                     /locus_tag="OO515_001180"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6575158.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35630..36562)
                     /gene="pxpC"
                     /locus_tag="OO515_001181"
     CDS             complement(35630..36562)
                     /gene="pxpC"
                     /locus_tag="OO515_001181"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6575159.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36556..37212)
                     /gene="pxpB"
                     /locus_tag="OO515_001182"
     CDS             complement(36556..37212)
                     /gene="pxpB"
                     /locus_tag="OO515_001182"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6575160.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37235..37978)
                     /gene="ybgI"
                     /locus_tag="OO515_001183"
     CDS             complement(37235..37978)
                     /gene="ybgI"
                     /locus_tag="OO515_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6575161.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38249..>39204
                     /gene="dtpD"
                     /locus_tag="OO515_001184"
     CDS             38249..>39204
                     /gene="dtpD"
                     /locus_tag="OO515_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6575162.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINA"
BASE COUNT         9527 a        10551 c         9514 g         9612 t
ORIGIN      
        1 ttgcgggatt atgctgcttt aatcattcag gatggtgggt cgtgcaggat tcgaacctgc
       61 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccat acgggtgtta
      121 cctgaaagat ttctactcgg ggccaatctt taagattggt gggtcgtgca ggattcgaac
      181 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccgagtggtg
      241 ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga
      301 gctaatcacc cgatactgcg ctggatacta caaactactt ccaccgaacc caccgtaatt
      361 agtggtgggt cgtgcaggat gactcgcttc gctcgcccta cgggccgttg ctgacgcaac
      421 gttatcctac acgcttaaca tctgtcttcg atgctaaaac tggtgggtcg tgcaggattc
      481 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt
      541 tttcgttgct ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc
      601 agacttgagc gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac
      661 acgaccagaa ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt
      721 tttgtgcctg tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg
      781 ctttcgcttt tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat
      841 cagcagcctt tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat
      901 catctttttt acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt
      961 aagttgaatc agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc
     1021 gggatttatc ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt
     1081 tcaccggcgc gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgccg
     1141 caccgctttg atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga
     1201 tctgcaacag gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct
     1261 gaatctgacc acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt
     1321 gggttaaaag ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga
     1381 ccgagcctga gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta
     1441 taccaaccag taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct
     1501 cttagtaaac cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta
     1561 ctgcaggttt ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac
     1621 cctgcaggta catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg
     1681 gagtaccacg ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca
     1741 ggaagtttgc atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca
     1801 gatcgaagta aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt
     1861 cggaagacat gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc
     1921 cttcgctgcc gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag
     1981 gcagagcaat catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac
     2041 tattcaatta attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca
     2101 gttgccggaa gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc
     2161 atcccctgag aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca
     2221 tccaggaacg tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg
     2281 tgctgctgcc caccgttgga gctgaccatt accataaatt taccgtcgct gctgacatcc
     2341 gcatcctggt tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc
     2401 actttataaa cctgtggacg accggcctgg tcagaagtaa atgccaggtt ctggctgtcc
     2461 gggaaccagg tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca
     2521 gaagccaaat ccattacgta caggttcagg ctaccggttt tcgacaaggc gaatgccagt
     2581 ttgctgccgt ctggcgagaa tgcaggcgca ccgttgtgac gcgggaatga agccacctga
     2641 cgtacagcgc cgtttgccag cgtttggata actagcgcgg aacgaccgct ttcgaaggtc
     2701 acataagcca gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgtggtgaa
     2761 cggtgaacga caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac
     2821 tgaccgccgt tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg
     2881 gtcagttttt caaacacttc atcactggcg gtatgaccag catagcgcag ccactgcttg
     2941 ttcactttgt acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt
     3001 tgataagcaa cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg
     3061 tcaataccca gcgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt
     3121 ggcagacgag cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc ggcagcaacg
     3181 atgccgccaa tatcttcagg tgccgcacca gggcccgccc actggaaagg aacaacacca
     3241 ataggacgac cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact
     3301 gatgcccaca gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc
     3361 ttatctggac cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa
     3421 actaccagaa ccccgtggca accggtgcct gatgttgacc gtcggaacag tcaacatcgc
     3481 gattacggtt tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt
     3541 ggtttcggga tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg
     3601 ccaccttcag gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta
     3661 caggttttgc ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc
     3721 tgcccggcat agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc
     3781 ccggcagacg aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca
     3841 gagcttagct caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt
     3901 tcagctgctg cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct
     3961 tttgccgctg ccgctttttt gtctgcggcg gctttttctg ccgctgcttt cttatcagct
     4021 gctgccttat cagcagccgc tttttctgct gcttttttgt cggctgcagc tttctctgct
     4081 gccgctttct ttcttgcttc agctgcagca gcttctgccg cttccgcttt cttcttcagt
     4141 gccgcagcgg ctgcctcggc ttttttctgc gcttcggctg cggctttggc ggcttctgct
     4201 tctgcttttt tcttcgcgtc tgccgccgct ttcttcgctg cttcttccgc agctttagca
     4261 tccgcttcgg ccttcgcttt agcatctgcc gccgctttcg ctgccgcctc ttccgcttgc
     4321 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ttcctgagca
     4381 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
     4441 agttcttcag cagcctgctg ttcctttatc ttgcgctgct catcagaacg cttcgcgctt
     4501 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
     4561 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
     4621 ctccagagca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
     4681 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
     4741 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
     4801 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
     4861 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
     4921 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
     4981 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
     5041 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
     5101 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gtacaatgtt
     5161 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
     5221 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
     5281 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg aggcggttat aagccattac
     5341 cgccggaatt gcggcgaaca gaccaattgc agtcgcaatc aacgcttctg cgataccggg
     5401 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
     5461 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
     5521 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
     5581 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
     5641 tttgaacccg ctgtaaaata tttgttccga accagtcaga ttatcccgtt tcccctgact
     5701 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
     5761 ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata ataatggccc aggatgcgat
     5821 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
     5881 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
     5941 caaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
     6001 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
     6061 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtacgtaata ttccaccgtc
     6121 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
     6181 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
     6241 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
     6301 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
     6361 cgcgcgattg aggtttggga atgagggcaa gttaagggag cgtaaaaatt tatgggcctg
     6421 gagcggccca taatttttta aagaaaattc tgatttaaaa gaagaagaaa atcagcccga
     6481 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
     6541 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
     6601 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
     6661 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
     6721 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
     6781 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
     6841 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
     6901 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaagc ggtgtagagc
     6961 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
     7021 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
     7081 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
     7141 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcgg aagaaccaca
     7201 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
     7261 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
     7321 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
     7381 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
     7441 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
     7501 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
     7561 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
     7621 agcggcggaa cgaaactacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
     7681 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
     7741 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
     7801 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
     7861 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
     7921 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
     7981 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
     8041 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
     8101 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
     8161 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga gttcgccaca
     8221 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
     8281 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
     8341 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccaga
     8401 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
     8461 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
     8521 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
     8581 accgcctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
     8641 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
     8701 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
     8761 tcctcttcct gatcaggaat gccgaacaga gtaaaggcag caggcgcagg ttgcgtttcc
     8821 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
     8881 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
     8941 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
     9001 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
     9061 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
     9121 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
     9181 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
     9241 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
     9301 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
     9361 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
     9421 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
     9481 tagaccgttt ccataatggc cagcaggaac gccataccga gcgtcagtgg cacaaaaagg
     9541 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
     9601 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
     9661 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
     9721 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
     9781 ttacgctgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
     9841 cggtgatcaa tttatagcta aattaccgcc tttcagccaa tttgatcgag aacaatttat
     9901 ctctttttga tgccaatttc caagacttat acattgataa atatcaattc ctacaaatta
     9961 tgttaatcat gtttcaataa ggtaacttta gtgacattta tgtttaaaat gtgtgagtta
    10021 taaggctgaa attagtttcg gttggcaatt taaagataaa aagagacgaa gcaaaaacag
    10081 cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt
    10141 actaataaag gcataaatcc accccgccga tattaacatt taattaactt gtaattatat
    10201 tataattaac tcacccaggg ttattatttt gttaaaaata aagccacata tacgtggctt
    10261 gagagtgaag ataaacgcaa aattacctga tgcgctacgc ttgccagtgt ctacatcatc
    10321 tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc
    10381 ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat
    10441 tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga
    10501 tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca
    10561 agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt
    10621 aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca
    10681 tcccactggg ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt
    10741 gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg
    10801 cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg
    10861 acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg
    10921 cccaggcctt aaaagcagat cttctttgcc cagtaagccc acgccacgca gcaacgtaat
    10981 ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtaaccctt cgctaaagac
    11041 agccatgccg ttacgccctt cctgtaaggc aacatagttg agcatattcc ataccggaac
    11101 cggcgcttct ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg
    11161 cgttagcgaa ccaaactgcg tatctgccag aacactgtca gtgttaaaag gtgtagggac
    11221 caggacacga aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatacg
    11281 cctgctgtta tgactaagag tgacaaccaa caccccccct actctgccag gcttcatgaa
    11341 taatatcgca ttgcggtttc gcgttcgctg cggtaattac ccactcttct tttgcgggtg
    11401 aatagtcata ttcatcacca tcatcagagc tttcttcaat ttgcaatacc cggtcatagc
    11461 gcacaccgct gtctttatct accagttgca gagaaccatc ctcattgagc gcaatctgcc
    11521 agaaagcatt ttccagaatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct
    11581 gattcgcttc aatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgaa
    11641 tatcaaactc cataaaggga tcataattgc cataatgaac aatttgccga tcgattaggc
    11701 caggatcgat ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctt
    11761 gtagattaaa ctggctggcg cgcagccgca cagtggtgtt aataacttct tcccgcggcc
    11821 agggcatcag gttaaacagc acgagtttgt cggcatcact ctgtggcatg ttatcggcaa
    11881 ttttgcgcat gtagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg
    11941 cgacaatttc gcgatgaact ttgtcactgc aacaacaacc gatactgtcg tgggcatgat
    12001 tttttaagat ctctttccac attttctcca gcaagccgtg gtggtattca aaacccaacg
    12061 tccaggccag cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt
    12121 gggcaatttt gatatccatg cgcgtggaac cgatggtgcg atgtacgcgc atatatttgc
    12181 catcgataaa ttcacctttc agggtggcca gactttcacg ctgcgcttcg atcttctcaa
    12241 atacctcttc aaagcggctc atcacaaact tacgttgagg gtagatctca cgtagcttat
    12301 ccatcacttc gaagatattt tgctgcaatg gcatctggtc atgaccattc ggcagcaaaa
    12361 tctctttggt tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttacgta
    12421 atccgttttc gtcggcaggt aagtacttac cgatggcgta gcccagcggc agcacctgcg
    12481 ccgtcacttc gctaccgtca ctgctttgcc acaaaaactc ggttttatca gtaccgtggc
    12541 gctccgaaca tccgcgccag aacatagtgc gggtaatgcc aaatccattg taaatatgcg
    12601 gcagttgccc ggacatgcca aaggaatccg gtaaataacc tattttcatc ggctcgccaa
    12661 acgcgaggca gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa
    12721 tcgtggtatc ggtctgggta taccaggggc cgataatcaa cttaccggct tctacctgtt
    12781 tcttcacgcg gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt
    12841 gcccgtcgag tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt
    12901 ccatattatt gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc
    12961 aatgcatatg cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt
    13021 ttatggcatt acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat
    13081 caggagaatt gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc
    13141 cgccataaca ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccggcacc
    13201 gggtgtcgaa aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag
    13261 cacaaacgaa ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt
    13321 aatacctgcc agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa
    13381 caggcgcggt gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga
    13441 ggcgaaaatg gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata
    13501 agcggctttg ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc
    13561 cagcaacagc gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat
    13621 ccatgcgaca ggttctccca ccacaaacag catcagactg cccgctccca acgtaccgag
    13681 caccgggtag agataaaaag tcaggaatcc attgaattta ctgctaagac gcaagtgatt
    13741 tttcacccag cgcatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc
    13801 ggagccgatc atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc
    13861 tgccagagaa taggtggtat aggccgcgag caccggtacc atcaaaattc cgagcagccc
    13921 gccgcccagc ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag
    13981 cccgaagatt tgcgacagta ataccgcgac cgccagcacc gtgccccccg cgacaatcaa
    14041 cggtacggca aaagagattc cgctcaacag tgcctgtttc agctccgttt tgacactttt
    14101 caccggttgc gtatcttgct gtacggtacg cgtctcatcg ctacgcttga gggtaagcgc
    14161 ttgttggatc aacgcttctg catggcgaat cggctcggca acaggcactg aaagcgcggg
    14221 aatgccatta aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcggt
    14281 tgcactattg agttgatccg ccgttaaacg cccttcaatg ccgttagcgc cttgtttttc
    14341 aacgtaaaca tttacgccga gtttgcgtcc ggctttttcc agatattccg cagccatata
    14401 ggtgtgagca atacccgccg gacaggccgt tacgcagacg atagttggcg cgttggaaaa
    14461 agaggcagaa ggttgcgtgc ctcccttgtc atccagcgcc gagagcaact catcaggcgt
    14521 cgtcgccgac tgtatacgcg cccgaatctc atcatccgca aggcgcgtgg tcagcgctgt
    14581 cagcagttgc atatgcgtag tacccgcttc attgggggga atcgccagca gcaccactaa
    14641 atcaactgct tccgggccat caacgccttc ccactgaagc ggctcgctga gtgtggcgac
    14701 cgcaaacgcc gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcacc
    14761 taaggcggtc gggccaaggc tttcacggcg atacacttct tccagaaatt gctcagtact
    14821 ggaaattttc cccagagcag caagacgttg agtcaacgcg tggatggcct cttcacggct
    14881 ggtaaagcgc gcattcagac acaacgcatc gcggtgggtc agagtcgtca gattcatcat
    14941 gatcctcact tgatcgtttt tatagtcgcg ccagtgtgcc cgataaaaca ataccatcat
    15001 gtgatcaatt tcacttgatt gaaattattt gtattgtatt tgtattattt acaggatgaa
    15061 tttatcgggc ataatggcta aattcatatt aatgagatac taaaaatggg acacaagccc
    15121 ttataccggc agattgccga tcgcattcgt gaacaaattg cgcgtggcga gcttaaacct
    15181 ggcgatgcgt tacccactga atcagcgcta caaacagagt ttggcgtcag ccgggttacg
    15241 gtgcgtcagg ctttgcgcca gctagtcgag cagcagatcc tcgaaagcat tcagggcagc
    15301 gggacttacg tcaaagaaga gcgggtcaat tacgatattt ttcagttaac cagttttgat
    15361 gaaaaactgt cggaccgtca cgtcgataca cacagtgaaa tcctgatatt tgaagtgatt
    15421 ccggctgacg attttcttca gcaacagcta caaatcaccg cgcaggatcg tgtgtggcat
    15481 gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc tggaagaaac ctggatgccg
    15541 cttgcgttgt tcccggatct cacctggcag gtcatggaaa attcgaaata tcactttatt
    15601 gaagaagtga agaagatggt tatcgatcgt agcgaacagg aaatcattcc tctgatgcca
    15661 accgaagaga tgagccgtct gcttaatatc agccagacaa agccgattct tgaaaaagta
    15721 tcgcgtggat atttagttga tggtcgggtg tttgaatata gccgcaacgc ttttaatacc
    15781 gatgactata aatttaccct gatagcgcag cgaaaatcat cgcgataagc acaaaaaagg
    15841 ccatcatacg atggccaacc atgtcgaaac cgacagccct tcgccattcc tgtagaggga
    15901 agcggcgagg gctatttctt attacagata tttatttcag aacagttttc agtgcttcac
    15961 cgatatccgc caggctgcga acggttttca cgcctgcggc ttccagagca gcgaatttct
    16021 catccgcagt ccctttccca ccagcaatga tcgcacccgc gtggcccata cgtttgcctt
    16081 tcggcgcagt cacaccagcg atgtaaccta caactggctt ggtaacgtgc tctttgatgt
    16141 acgctgccgc ttcttcttca gcgctaccgc cgatctcacc gatcatcacg atcgcttcgg
    16201 tctgcggatc tttttcgaac atttcgagga tgtcgataaa gttagagcct gggatcgggt
    16261 caccgccgat accaacacag gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg
    16321 cttcataggt cagtgtaccg gaacgggaaa cgatacccac tttacccggt ttgtgaatgt
    16381 gacccggctg gataccgatt ttgcattcac ccggagtgat aacgcccggg cagttcgggc
    16441 cgatcatacg aacgccggct tcgtccagtt tcactttcac agtcagcata tccagcgtcg
    16501 ggatgccttc agtgatggtg ataatcagtt tgatgcctgc atcaatagct tccagaatgg
    16561 agtctttgca gaacggtgct ggtacgtaga taacagaagc ggtagcgcca gtagcagcaa
    16621 cagcttcacg cacggtgttg aacaccggca ggccgaggtg ggtagtaccg cctttacctg
    16681 gggttacgcc gccaaccatt ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc
    16741 cctggctacc agtaaagccc tggcagataa ccttggtgtt tttatcaatt aaaatggaca
    16801 ttatttcccc tccactgcgg caacaacctg ctgagctgca tccgtcagac cttttgctgc
    16861 aataatattc aggccgctgt cagccagttt cttcgcgccg agttcggcgt tgttaccttc
    16921 cagacgtacc acgaccggta cgttaacacc cacttctgct accgcgccga tgataccgtc
    16981 agcgatcagg tcgcaacgaa cgataccgcc gaagatgtta accagaacgg ctttcacttt
    17041 gtcgtcagag aggatgattt tgaacgcttc ggttacacgt tctttggttg cgccgccgcc
    17101 aacgtcaagg aagttagccg gttcgccgcc gtgcagttta acgatgtcca tcgtacccat
    17161 cgccagacct gcgccgttaa ccatacaacc gatgttaccg tccagcgcaa cgtagttcag
    17221 ttcccactgt gcagcctgtg cttcacgcgg atcttcctgc gactggtcac gcatttcgcg
    17281 cagatcaggc tggcggaaca gtgcgttgcc gtcagcgccc agtttgccgt cgaggcaaat
    17341 cagatcgccc tgtttggtga tgaccagcgg gttgatttcg atcaacgcca ggtcgcgctc
    17401 caggaaaatg gtcgccaggc ccatgaagat tttggtgaac tgctgaacca gtttaccttc
    17461 cagacccagt ttgaacgcca gctcgcgtcc ctgatacggc atcgggccag tcagcggatc
    17521 aagcgcaact ttgtggatca ggtgcggagt ttcttccgcc actttttcga tttccacgcc
    17581 gccttcggtg gaggccataa agaccacacg acgggaacta cggtctacaa cggcaccgag
    17641 atacagctct ttagcgatat cggtcgctgc ttcaaccaga atctggttaa ccggttggcc
    17701 attggcatct gtttgatacg ttaccagacg cttgccgagc cagttttctg caaaagcacg
    17761 gatgtcttct ttgctgttta caactttcac accgcccgct ttaccgcggc caccagcgtg
    17821 aacctgacat ttcactaccc acgggccggc accgattttt gaagcggctt cttctgcttc
    17881 gcgcggagta gtacaggcat aacccaccgg tgctggtaag ccatagcggg caaaaagttg
    17941 ttttgcctga tattcatgta agttcatgtg ttctgtccat ccttcagtaa tcgttatctt
    18001 ttaaaccgta gaccgacacc ctatccacgt cggtctacag tgcaggtgaa gcttaaacta
    18061 ctacacgtcc agcagcagac gcgtcggatc ttccagcaac tctttgatcg ttaccaggaa
    18121 gcccacggat tcgcgaccat cgatcagacg gtgatcgtag gacagcgcca ggtacatcat
    18181 cggcaggatc tcaacctgac cattcaccgc catcggacga tctttgatag cgtgcatacc
    18241 cagaattgcg ctctgcggcg ggttgatgat cggcgtagac atcagggaac cgaacacacc
    18301 accgttggtg atggtgaagt taccaccggt cagatcttca accgtcagct tgccgtcacg
    18361 gcctttaact gccagctctt tgattttctt ctcgatgtct gccatgccga gggtatcgac
    18421 atcacgcagt accggcgtca ccaggccgcg cggcgtggaa accgccatgc tgacgtcgaa
    18481 atagttgtgg taaaccacgt catcaccgtc gatagaagca ttcacttccg ggtaacgttt
    18541 cagggcttca accaccgctt tcacgtagaa ggacataaag cccagacgga tgccgtggcg
    18601 tttttcaaac gcttcaccgt actgcttacg cagatccata atcggcttca tgttgacttc
    18661 gttgaacgtg gtcagcatgg cggtggagtt tttcgcttcc agcagacgct ctgccacacg
    18721 cttacgcagg cgagtcatcg ggacacgttt ttcactacgt gcagccagag ccggttgcgc
    18781 cgccggagca gccgctgccg gagcagactc tttcgccggg gctttcgcca gatgtttttc
    18841 cacatcttca cgagtcagac gaccacccac accggtgcct ttaatggcgc tggcgtcgag
    18901 attgtgttca gccagcaggc gacggatcgc cgggcttaac gcatcgttgt tttgctcttc
    18961 cagagacgcc tgctggcgtt gcgccggagt ggacgctttc tcttcagatt tggcgctggt
    19021 ttctttaccg gcgctgttgc cttcacgcag gcgaccaagg atctgacgag acgttaccgt
    19081 tgtaccttca tcttccagaa ccgcatccag aatgccgtct gctgatgccg gtacttccag
    19141 taccactttg tcagtttcga tttctaccag cacttcatca cgtacgactg cgtcgccggg
    19201 ttttttatgc caggttgcga cggtggcatc ggctacggat tcaggcaggt cagggaccag
    19261 aatatctacg ctactcattg tgtatccttt atttattcga cgttcagcgc gtcattaacc
    19321 agatcttgtt gctgtttctg gtgaacggac atatacccta ccgccggaga ggcggaggcc
    19381 gtgcggcctg cataacgcag agaagcccca aacggaatca cttcacggaa atgatgctgg
    19441 ctgcagtacc atgcgccctg gttgagcggc tcttcctggc accagacaaa atccttgacg
    19501 tgagcaaact gctgcaacac ttcctgcatc gctttatgcg ggaacgggta gagttgctcg
    19561 atacgcacaa tggcgacatc gtgttgattg ttcttacgac gctgttccag caggtcgtaa
    19621 taaaccttac cagaacacat cactacgcgc ttcacgccct tcggatcaag ctcgtcgatt
    19681 tcaccgatgg ctggcaggaa ggtgccgttc gccagttctt cgaggctgga aaccgccagc
    19741 ggatgacgca gcagggattt cggcgacatc actaccagcg gacgacgcat cccgcgcagc
    19801 gcctgacgac gcagcatgtg gtaaacctgt gccggggtag acggaacgca aacctgcatg
    19861 ttttgctcag cacaaagttg cagataacgt tccagacgcg cggaggagtg ctccggcccc
    19921 tgcccttcgt aaccgtgcgg cagcaacatc accagaccac acatccggcc ccatttctgt
    19981 tcgccagaag agatgaactg gtcgataacc acctgcgcac cgttggcgaa gtcaccgaac
    20041 tgcgcttccc agatggtcag agtgcgtggt tctgcggtgg cataaccgta ttcaaacgcc
    20101 agcactgctt cttcagacag tacggagtcc cagacgcgga acgcgccctg cccgttatgg
    20161 atgtgttgca acggcgtgta agtggaaccg ttagactggt tgtggatcac cgcgtggcgg
    20221 tggaagaagg taccgcgacc ggagtcttca cccgacaggc gaaccggaat gccttcgtca
    20281 accagcgtgg cgtaagcgag gttttccgca ccgccccagt cgaacagttt ctcaccggca
    20341 gccatcgcct ggcgatcgcc ataaatcttg gcaacgcgag actgcatttc aactgcttcc
    20401 ggcaccgtgc tgatgcgttt cgccagctcc tgcaggcgct tcatctcaac tttgttcggg
    20461 tactcttcgt cccattcgtg gttgaggtac ggcgaccagg tgaaagagtg catgttcatc
    20521 ggacgccact ctgccactac gcaatcgcca gcatccagcg catcgcggta caggttaacc
    20581 atctcggtgg catcttccag cgtcgccact ttttcctgct ccagcttgtc agcgtagatt
    20641 ttgcgcggtg tcggatgttt tttgattttc tgatacatca gcggctgggt tgcgctcggc
    20701 tcgtcggctt cgttgtggcc gtgacggcgg tagcacacca ggtcgatgaa gacatcacgt
    20761 ttaaaggtgt tacggaaatc gagcgccaga cgggtcacaa aggcaacggc ttccggatca
    20821 tccgcgttaa cgtggaaaat cggtgcctga accatcttac cgatatcagt acagtacggt
    20881 gtagaacgag catccagtgg attagaggtg gtgaaaccaa cctggttgtt gataacgata
    20941 cgtaccgtac cgccaacttc ataaccacgc gctttcgaca tgttcagagt ttcctgaacc
    21001 acgccctgcc cggtcactgc ggcgtcaccg tggatggtga ttggcagcac tttgttgctg
    21061 ctcggctcat caagtctgtc cagacgggca cgaacagaac cgataactac cgggcttaca
    21121 atctcaaggt gagacgggtt aaacgccagc gccaggtgca ccaggccgcc atcggtctgg
    21181 aagtcagacg agaagcccat gtggtatttc acgtcacccg tgccgaggtg ttctttatgt
    21241 ttaccggcaa actcgtcgaa caagtcttgc ggttttttac ccagcacgtt caccagcacg
    21301 ttcagacgac cacggtgcgc catcccaaga accacttcgc gggtgccgct gttgccagcg
    21361 tggcggatca tctctttaag catcgggatt aacgcgtcac cgccttccag cgagaagcgt
    21421 tttgcgccag ggaattttgc gccgaggtaa cgttcaaggc cttcagcggc ggtcagttcg
    21481 cttaagaagc gttttttctc ttcgctattg aaagtcgcgc gaccagactc gatacgctgt
    21541 tggatccagc gtttttcttc ggtgctggta atgtgcatat actcggcacc aatcgggcca
    21601 cagtaggttt gcttgagggc ttccagcagc tcgccgagtt tcatgttttc tttgccgctg
    21661 gcaaatgaac cgacgttgaa ggtctcctgg aagtctgctt cggtcagatc gtggaaagac
    21721 ggatccagat cggccacttt atcttgctgc cacagtccca gcggatcgag attcgcatgc
    21781 tggtgaccac ggaagcggta tgcgttaatg agctgcagga ctttaacctg cttcacattg
    21841 gtgtcagggt cggagatcgt tgaagagtaa cgtgaagcgt ctttcgccag gcggcggaaa
    21901 tattcacgcg tttgagagtg gaattgatcc ggtttgactc ccgtaccagg taactgctgg
    21961 aacgtcgaac gccagttagc gtcaaccgag tcaggatcgg ttaagaagtc ttcatagagc
    22021 tgttctatcc agctctggtt tgcgccagag aggtaagaag agtccaacca ggctttcaaa
    22081 gcgctgttct gcatcgtgat cccttaagca tcttttttat gcttacttcg ccgtggatac
    22141 taccacgcac agtgatgtgc gtgccggggt tcacttgcga gctcttgtct atgtagtggc
    22201 tcgcgaagga acctttaaaa actgtcttag tgtcaggggc agtttttaaa gatttcctgc
    22261 atttctttgt tacgcctgat gcgctgcgct tatcaggcct acgggtaatg acttgtaggc
    22321 cggataaggc gttcacgccg catccggcac tggttgcctg atgcgacgct tgcgcgtctt
    22381 atcaggccta cggtttacgc attacgttgc aacaacatcg acttaatatg gccgatggcg
    22441 cgcgtcgggt tcagcccctt cggacataca ctgacgcagt tcatgatgct gtgacagcgg
    22501 aatacgctga atgcatcgct caaaccgtcg aggcggctgt cagtctcggt atcacggcta
    22561 tcgatcagga aacgatatgc cgctaacaag cctgccgggc cgataaactt atcgggattc
    22621 caccagaaag acggacaaga ggttgaacaa catgcgcaga gaatacattc atacagcccg
    22681 tcgagttttt cgcgctgctc tggcatctgt aaatgctcgc gagctggcgg attttgtcca
    22741 ttattcaaca ggtaaggctt aattttctca tattgcgcat agaattgtcc catgtctacc
    22801 accaaatcgc ggatcaccgg taaacctggc agcgggcgaa tcacaatctt cttgcccggc
    22861 tggttgagtg ccgaaatcgg ggtaatacag gccagaccat tcttgccgtt catgttcaga
    22921 ccgtcggaac cgcacacacc ttcacggcag gagcggcgga acgacaggct gggatctttc
    22981 tctttcagct ggataagcgc atccagcagc atcatgtcgc gaccttcttc cgcttccagg
    23041 gggtaatcct gcatacgcgg agcatcatca acatccgggt tatagcgata aattgaaaac
    23101 tcgagtctca ttttcctgtc tccgcattag taagtacgaa tcttcggcgg gaatgccggg
    23161 cgcagtttcg gttccatgtt gacgcttcgg cgcgtcatgg attccgactc tggcagatac
    23221 agggagtggc acagccagtt ttcatcatca cgatccggga agtcgaagcg gctatgtgcg
    23281 ccacggcttt cggtacggaa gttggcagaa acagccgttg catacgccgt ttccatcagg
    23341 ttatccagtt ccaagcactc aacgcgctgg gtgttgaact cgctggaagt gtcatccaga
    23401 cgggcatttt tcagacgctc gcggatcact ttcaactgct caagcccttt cgccatcgca
    23461 tcaccttcac ggaagaccga gaagttatgc tgcatacatt cttgcagcgc tttacgaatc
    23521 gccaccggat cttcaccgtt acgattattg ttccagcggt gcagacgatc cagagatgct
    23581 tcaacatcag actcgctggc atcgcgcaat gcgccctgtt cggcgataga ctcttgcaga
    23641 tgcagacctg ccgcacgacc aaagaccacc aggtccagca gcgagttgcc gcccagacgg
    23701 ttagcgccgt gtaccgatac acaagcgatt tcaccaacgg caaacagccc cggaacaacc
    23761 acatcttcgc ctttctcgtt caccgtcagc gcctgaccgg tcactttggt cggaataccg
    23821 cccatcatgt agtgacaggt tgggataacc ggaatcggct ctttcaccgg atcgacgtga
    23881 gcaaaggtac gggaaagctc caggataccc ggcagacggg attcgagaac ttctttaccc
    23941 aggtgatcga gtttcagttt cgcgtgtggc ccccacggac catcacagcc gcgaccttca
    24001 cggatttcaa tcatgatgga acgcgccacc acatcacggc ccgccaggtc tttggcgttc
    24061 ggcgcataac gctccataaa gcgttcgcca tgtttgttca gcagataacc gccttcacca
    24121 cggcaacctt cggtgaccag tacgcccgca ccggcaatgc cggtcgggtg gaattgccac
    24181 atttccatat cctgcaccgg tacgcctgcg cggatagcca tgccgacacc gtcgccagtg
    24241 ttaatgtggg cgttagtggt ggactgataa atacgccctg ccccgccagt cgccagcacg
    24301 gtagcgcggg ctttgaaata aaccacttca ccggtttcga tgcacagtgc ggtacaaccc
    24361 accaccgcgc catcctggtt tttcaccaga tccagcgcat accactcgga gaaaatggtg
    24421 gtgtggtttt tcaggttctg ctgataaagc gtgtgcaaca gtgcgtgacc ggtacggtca
    24481 gccgccgccg cagtgcgtgc cgcctgctcg ccgccgaagt ttttcgactg accgccaaac
    24541 ggacgttgat agatacgacc atcatcaaga cgcgagaacg gcaggcccat gtgttcgagt
    24601 tccagaatcg cttccggccc ggttttacac atatattcaa tcgcgtcctg gtcaccgata
    24661 tagtccgacc cttttacggt gtcgtacata tgccattccc agttatcttc atgggtatta
    24721 cccagcgcaa cggtaatgcc gccttgcgca gaaacggtat gggaacgggt cgggaagact
    24781 ttagagagca gcgcacaggt ctggccgctc tgggaaattt gcagcgccgc gcgcatacct
    24841 gcgccaccgg caccaatcac aactgcatca aattctctga ctggcaattt catcacacac
    24901 cccacaccac aacgaatcca taaatcacgt aaaccaccag tgcaacgaca atcaccagtt
    24961 gcagcatcag gcgcaatgcc agcggtttaa cgtagtcggt caacacctgc cacatgccga
    25021 tccaggcatg aatcaagata gaaaacagcg ccagcagggt gaacactttg gtgaacgcag
    25081 aggcgaagaa accgatccag acttcatatg tcagttcgcc actggtggcg aaaaaaccga
    25141 ccatataaat gatgtagagc gtcaggacga tagcggtagc gcgaacgagg atgaaatcat
    25201 gtacgccatt gcgtcctaat gcggaggcgt tgcttaccat acgaggactc ctgcgagaag
    25261 tgaaagcacg acagtaataa caaaggagat tttggcggag cgtttacccg cttcgaatgt
    25321 ttcttccaga tagccaaaat ccatcatcat gtggcgaata cctacgacga cgtgatacgc
    25381 cagagcggta aggatgcccc acatgataaa tttgacgaag aagctgccca taatcgcgga
    25441 agcttgctcg aaaccttcag gggaagagag gctggtaccc agaagccaca gcaggatgcc
    25501 cactgcaaca aaggtgatca caccggaaac gcgatggaga atggacgcta tcgccgtgat
    25561 ggggaaccgg atggtctgta ggtccagatt aacaggtctt tgttttttca catttcttat
    25621 catgaataac gcccacatgc tgttcttatt attccctggg gactacgggc acagaggtta
    25681 actttctgtt acctggagac gtcgggattt ccttcctccg gtctgcttgc gggtcagaca
    25741 gcgtcctttc tataactgcg cgtcatgcaa aacactgctt ccagatgcga aaacgacacg
    25801 ttacaacgct gggtggctcg ggattgcagg gtgttccgga gacctggcgg cagtataggc
    25861 tgttcacaaa atcattacaa ttaacctaca tatagtttgt cgggttttat cctgaacagt
    25921 gatccaggtc acgataacaa catttattta atttttaatc atctaatttg acaatcattc
    25981 aacaaagttg ttacaaacat tgccaggaaa agcatataat gcgtaaaagt tatgaagtcg
    26041 gtatttcacc taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga
    26101 cagttattag tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt
    26161 attcgtcatc aatggatcct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc
    26221 ctctttcaca gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg
    26281 caaatttaag ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat
    26341 acaaaagcaa aactcaccct caacggggac acagctgttg aactggatgt gctgaaaggc
    26401 acgctgggtc aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt
    26461 gacccaggct tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat
    26521 gaaggtattt tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac
    26581 ctggaagttt gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa
    26641 tttaaaacta cggtgacccg tcataccatg atccacgagc agattacccg tctgttccat
    26701 gctttccgtc gcgactcgca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg
    26761 gcgttctatc acgactcgct ggatgttaac aatcctcgtc accgtgaaat tgccgcgttc
    26821 cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag
    26881 ccatttgttt atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc
    26941 tccacgccgt gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt
    27001 ctgatcctgc acgctgacca tgaacagaac gcctctacct ccaccgtgcg taccgctggc
    27061 tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct
    27121 gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa
    27181 cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc
    27241 ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc
    27301 catgaagttc tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg
    27361 gaaaacatcg cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat
    27421 ttctactctg gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatt
    27481 ttcgcaatgg cacgtaccgt tggctggatc gcccactgga gcgaaatgca cagtgacggt
    27541 atgaagattg cccgtccgcg tcagctgtat acaggatatg aaaaacgcga ctttaaaagc
    27601 gatatcaagc gttaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcaaatg
    27661 gcgggttaat ttttatatgc ccaaacacaa aaaattgtaa aaataaaatc cactatcgga
    27721 cattcatata ttctaaaaaa aatatatcgg tataaattat aaacaaatcg atgattttta
    27781 atcatctatc ttcctctcag ttacatgaat aaaccaccaa atatagcgca cccctataat
    27841 ttccaatact ttattcatca aacatttaat tcataccatc aattaattac ttttacattt
    27901 aataaaatta ataatatttt taagaaaaac taaaaacatt cgacaatcaa ataagatatt
    27961 cttgcaccga tatacttttt tcttttctgt taaaggactc agttatgttt aaaggacaaa
    28021 aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat gcggctgatg
    28081 aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt gaaatacatc
    28141 aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac attaatcagt
    28201 cacatcacag cgatgccgtt gctgtcgacc tgcgcttagt caactgtgat ctggaaaact
    28261 ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat agctcagcga
    28321 aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc accggcgtcg
    28381 gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct actccagata
    28441 tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa
    28501 ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc tacgtgctgg
    28561 attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat atttatcagg
    28621 tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc
    28681 ttgcgcattg gcggtcgaat tcaaccttaa tgttctcgat aaatcgatgc gcgaccgcat
    28741 tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt
    28801 tgcggtaaat aataaccaaa tcagtaacgg gcaaaagatt aactggcaca aaaatgacga
    28861 taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa aacctgaagt
    28921 ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac ctgaaatgct
    28981 cttcaatttc gatcaagcca atcagcaact gaatattagt attccgcaag cctggctggc
    29041 gtggcactca gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg ccggtatcct
    29101 gatggattac aacttgtttg ccagcagcta ccgcccacag gacggcagca gcagcactaa
    29161 cctgaacgcc tacggtacca ccggaattaa cgccggggca tggcgcttac gtagtgatta
    29221 tcagttgaat cagactgata gcgatgataa ccatgaacag tcaggcggaa tatcgcgcac
    29281 ctatcttttt cgtccattac cgcaattagg ctctaaatta accctcggcg aaacggattt
    29341 tagttccaat attttcgacg gtttttctta taccggcgcg gcactggcaa gtgacgagcg
    29401 aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg cacagaccaa
    29461 tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag tcccaccagg
    29521 cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg tcaaagtgac
    29581 ggaagaagat ggtcgggtga acaatttcca ggtttcggca gcatcgacgc ccttcctgac
    29641 tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct ccatgtcaca
    29701 tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac
    29761 ctcgctgtac ggcggcctgc tgctttctgg tgatgactac cattctgccg caatgggtat
    29821 tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca
    29881 ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca gcaaacaagt
    29941 ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc gtcattttca
    30001 cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg acgaaaaaca
    30061 gacgatcagc ttattgtggg ccaaccgatt accccactaa acctcaatct ttacgccaac
    30121 ctgctacatc aaacctggtg gaatgcagac gcctccacga ccgccaacat cacggcaggt
    30181 tttaatgttg atattggtga ctggagagat atctctattt cgacgtcatt caatacaacc
    30241 cattacgaag ataaagatca cgacaaccag atttacctgt cgatttcgct ccccttcggt
    30301 aacggtggtc gggttggcta tgacatgcaa aacagtagcc acagcaccac acaccgcatg
    30361 tcgtggaacg atacgctgga tgaacgtaat agctggggca tgtctgccgg actgcaatcc
    30421 gaccgtcctg acaatggagc ccaggtgagc ggtaactatc agcacctgag ttcagcgggt
    30481 gagtgggata tttctggtac ctatgccgcc aatgattaca gttccgtcag cagcagctgg
    30541 agcggttctt tcaccgcaac ccaatatggt gcagcgtttc atcgccgcag ctccaccaat
    30601 gaaccacgcc tgatggtcag caccgatggc gtggcagata ttccggttca gggcaatctc
    30661 gactacacca accattttgg cattgcggtg gtgccgttga tttccagtta ccagccttcc
    30721 accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa cagttgcaga aaacgttatc
    30781 aaagaaacgt ggattgaagg cgcgataggt tacaaatcac tggcttcccg ttccggtaaa
    30841 gacgttaacg tcatcattcg caacgccagc ggtcagtttc ctcccctcgg agcggatatc
    30901 cgccaggatg acagcggcat tagcgtgggg atggttggcg aggaaggaca tgcctggtta
    30961 agcggagtcg ctgaaaatca aaagtttacc gtggtctggg gtgatagcca gcattgctcg
    31021 ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc tgattttacc ttgtcattaa
    31081 ttaaggaaat gaaaatgaca tttatgaaag gactacccct tttgttgttg gttgccagtc
    31141 tgtgcagcca cgctgcacta caacccgatc gcactcgtat tgtgtttaac gctaatgata
    31201 aagctaccag tctgcgggtg gataatcgta gcgataaact cccctacctc gcttattcct
    31261 ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct ggtcgccctg ccgccgatac
    31321 aacgtctgga accgaaagca acaacgcagg tacggatagt aaagcaagcc tcgaccacaa
    31381 aattaccggg cgatcgcgaa acgctatttt tctacaatat gcgtgaaatt cctcccgccc
    31441 cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat tcagagtcgt atcaaggtgt
    31501 tctggcgacc ggctgcatta cgcaagaaag caggagaaaa ggtcgaactg cagttacagg
    31561 tcagccagca gggcaaccaa ctgacactga aaaatcctac cgcgtattat ctgaccattg
    31621 cctacctggg acgaaatgaa aaaggcgttc tccccggctt caaaaccgtg atggttgcgc
    31681 cttttagtac ggtcaacaca aacaccggaa attatagcgg cagccagttt tatctcggtt
    31741 atatggatga ttacggcgcg ttacgcatga cgacgctcaa ctgcagcgga caatgccatt
    31801 tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga gtgttgctcg tcatctgtct
    31861 gttatttctg ccgttgaagt ctgcgcttgc cctgaactgc tactttggtt catccggtgg
    31921 ctcggtagaa aaatcagaag cgattcaacc ttttgctgta ccaggcaatg ccaaactcgg
    31981 cgataagatt tgggaatctg acgatattaa aattcccgtc tattgtgaca acaataccaa
    32041 cggcaatttt gaaagtgagc atgtttatgc ctgggtaaac ccctacccgg gggtgcagga
    32101 tcgctattat caactcggcg taacttacaa tggcgttgat tatgacgcca accagggaaa
    32161 aagccgcatc gacactaacc agtgtattga cagtaagaac attgacattt atacccctga
    32221 gcagattatc gcgatgggat ggcagaacaa aatttgctcg ggtgatcccg ctaatattca
    32281 catgtcgcgt acctttctcg ctcgcatgcg gttatacgtc aaaatacgag aaatgccgcc
    32341 gcatgattat caaagtacgc ttagcgacta tatcgtcgtg caatttgacg gtgccggtag
    32401 cgttaacgaa gaccctactg cccaaaacct gaaatatcat attactggtc tggaaaacat
    32461 tcgcgtgctg gattgcagcg tcaatttttc catttcaccg gaaacacagg tgattgattt
    32521 tggtaaattt aatttgctgg atattcgtcg ccacacaatg tcgaaaacat tcagtattaa
    32581 gacgacaaaa tcacaaaatg accaatgcac cgacggattt aaagtcagtt cctcctttta
    32641 taccgaagaa acgttggttg aagaagataa agcattgttg attggtaatg gtctgaagct
    32701 acgtttgctg gatgaaaacg cctcgcctta taccttcaat aaatatgctg aatacgccga
    32761 tttcaccagc gacatgttgg tctacgaaaa aacctataca gctgaacttt cgtccattgc
    32821 aggtaccccc atcgaggctg ggcccttcga tacagtggtg ctttttaaaa ttaactataa
    32881 ctgatttgcc atgatgccct ttgcagccgg aaagggcatc tctttcagaa aattgctgat
    32941 tttccccttt tccttgattg ctgttatttt ttcctggccc ggctttcttt atgctgtatc
    33001 gatacaccaa agagaaaata atgagggagc gaaggatgcc agttttgcag tggggaatgt
    33061 tatgtgtgct atcacttctc ctttctattg gtttcctcgc ggtacatctc ccggcggcgc
    33121 tattacttgg gccgatgatc gccgggatca tcttcagtat gcgcggaatt actctgcaac
    33181 ttccccgctc cgcttttctc gccgcgcagg ccattcttgg ctgcatgatt gcgcaaaacc
    33241 tcactggttc gattctcacc acgctggccg ccaactggcc gatcgtgctt gctattttac
    33301 tggtgacgct gctttccagc gccatcgtgg gttggttatt ggtgcgctat agctcactgc
    33361 ctggaaatac cggtgcctgg ggttcttctc caggcggcgc agcggcaatg gtcgctatgg
    33421 cgcaagatta cggcgcagat attcgcctgg ttgcatttat gcaatatctg cgagtgctgt
    33481 tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct gggtgataac gctgaagcgg
    33541 ttaaccagca aatcgtctgg ttcccgccag tgagcattaa tctcctgctt acgattttgc
    33601 tcgcagtcgt tgccggtacg gcgggatgca tgctgcgtct tccttccggc acgatgctca
    33661 tcccgatgct ggcgggcgca gtgctccagt ctggtcagct catcaccatc gaactaccgg
    33721 aatggctgct ggcgatggcg tatatggcaa ttggctggcg gattggtctt ggtttcgata
    33781 aacaaatctt actgcgggca ctacgcccgc taccgcaaat cctgctgtcg atttttgctc
    33841 tgctggctat ttgtgcgggt atggcgtggg ggctgacccg gtttatgcac attgatttta
    33901 tgactgccta cctcgccacc agtcccggcg ggcttgatac cgtggcggtc atcgccgcag
    33961 ggagcaatgc cgatatggcg cttattatgg cgatgcaaac cctgcgcctg ttcagtattt
    34021 tgctgacggg gccagccatt gcgcggttta tttcaaccta tgcgccgaag cggtcggcct
    34081 agtgctggca gccagggcac cagtaaaacg ggcgagatga cagcgtggtt ttctcaatga
    34141 tgctgccaca gcgttcgcac ggttcgccat cacgatgaaa aaccttaaag cgaaacagcg
    34201 ccccatgatg cttattctca tccaccagcc ccctcgtagc gtaggaaaat cgaggaatat
    34261 ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc gctttatgat
    34321 ttccagtcaa cccaacctgc cagaggatct ccacccgcaa ataattgcca agcccagcca
    34381 gaaacgcctg atcgagcagt aatcctgcaa actgacggtt acgaaagcgc ggtgacaata
    34441 accgttcctt taccacctcc ggcgtcagat tcggatccag cacatcggga ccaacgcgtt
    34501 gtaaaaacgg atgcgtggtc agttgttccg gcctcaacat ctcaatatcc gaggcgctat
    34561 aaagcagaat ggttttgtca gccgtttgca gttttacccg caatactcgc gtggtctgcg
    34621 gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg ctgtagagcg
    34681 ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg cgtttccaca tgggtaacgt
    34741 gttgaccgat aagttgtgat tgataagttt ttaactgcgg gaaggcaaac cagacatcag
    34801 ttagtggttt gcctttgatc gccgcctcca ggttatccgc tgcacggcgg atctccgggc
    34861 cttcaggcat ggtgatatcc ttttttcatt ctttattaat cggggttatg ctgcgacaac
    34921 aatctccttt tcggcaaatg tagagcgtag tcggcgggcg aacgccagtg cgtgctcgcc
    34981 gtcgccatgc aggcagactg tttgcgccgt gaccgttgcc cattcgccgg tgatactttt
    35041 gactctgccg tgttgcacca tctctagcgt ttgcgccaac gcctgttctt cgttttcaat
    35101 caacgcgcct cgctgacttc gcggcaccag cgagccgtca gcctgataac cgcgatcggc
    35161 aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct gcacgaatca gctcgcttcc
    35221 tgccagccca acgagaatca atgccggatc gcaagcgtat accgctctgg cgatggcgtc
    35281 tgccagttgt gcttctttcg ccgcctggtt gtacaacatg ccgtgcggtt tgacatgacg
    35341 cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg ccaatttgat acagcgtctg
    35401 ggcgtaaacg gtttctggcg gcagctgcat ggcgctgcga ccaaaatttt ccctgtcggg
    35461 aaaactcggg tgagcgccaa tcgcgacacc attttttatt gcttcacgca cgcaagcctg
    35521 catgatttgc gcatcgcctg catgaaatcc acaggcaata ttggcagagg aaaccagcgt
    35581 taatagctct gcgtcgctgg cgcagccttc gcccagatcg gcgttcaggt caattttcat
    35641 tgtgcagccg ccacactaat tgttcgaaat aacgttgctg atcttgccgc gctttcagtg
    35701 cctcttccag tgaacactgg acaaaatgaa tcggctgacc gagcggaatt tgcgccagat
    35761 ggtacatatc agcctcaatg atacaggcaa tacgcgggta accatcggtg gtctgtgcgt
    35821 cgttcatcaa cacaatcggc tgcccgttat gtggcacctg caccacgccc ggtaacaaac
    35881 cgtgagataa cagttcgcga tcggtggtgc gttttaaaat ttgcccctgt aagcgatagc
    35941 ccatgcggtt actttgcgag ctaagctgcc agggcgaacg ccagaatgca tcctgcgagg
    36001 cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg aatgcggttg ccccacagca
    36061 gctgtttaac gccctgcgct tccataaaat cacgcttcga tttgccaatc gggagtcggt
    36121 caccatcctt cagtaaacgg ccttccagcc cgccaatccc cactttgaga tcggtgctgc
    36181 atgaccccat taccggcgga acatcaatac cacccgcgac cgccagataa ctgcgcatcc
    36241 cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt catcggcaat cgccagccgg
    36301 tccagacggc attatcatcc agccgcgctt cgcaaccggc acccgtcaga gcaaaccacc
    36361 catcagtttc gaactcaacc gttaactgac cgagcgtgat ctccagcgcg ggggcattgg
    36421 cgtcattacc caccagcagg ttagcaatgc gtaacgcagg catatccagt gcgccgcagt
    36481 ggctgattcc cgactggcga aaaccgtgac gaccgccatc ctgcacagtg gtatacatgc
    36541 ccgcacgaat aatcttcagc atactccctc cttctgcggt acaaagcgca cgctgtctcc
    36601 cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac aggctgagtg aggtatgacc
    36661 aatcaactgc cagccacccg gcgttgccag cggataaaca ccagtctgcg gcccgccgat
    36721 cccgacagaa cctgccggaa cgagcaagcg cggttcagcg cgccgtggcg tgtgtagttg
    36781 ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc agaccacgta
    36841 ttccacggag gagtgcaatt caacaacctg tttttcgcta aacccgcaat gcgccgcgac
    36901 caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc ggaatttcaa taaagcgaga
    36961 ctcaggttcc agggcctcgc tctcctccca ccagcgttgc aaacgctcta tggcatccag
    37021 cgccagcgac tcaggattac gcaaaatcac cgtgatattg ttcatgccgg gaatggcttc
    37081 aaccacattc ggcatatcca ccagacgctg cgccagtcgc cagatccgtt tctggctagc
    37141 cagcgtcacc ggcggttcca gttccagcac taccgccgtt tcacctatca gataacaacg
    37201 cgctcgttgc actttcgatc cctctgatta ttcgttatgc aggattagga atatcaataa
    37261 aggtcacatc aagatcggta ttttcattca gccactcgct caatgcgcga ataccaccac
    37321 gttcagtggc atggtgacct gcagcataaa aatgcaaccc ctgctcgcgg gctgaatgaa
    37381 tggtctgttc agaaacttcg ccagtaataa aagcatccac gccaaaacgc gcggcgctat
    37441 cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg ctggaccacc tcaggtccgg
    37501 tatcgccaca ccataacggc ttacgtccca gacgcgcttc aatccaggag gctagttcca
    37561 gtcccggcac aggcatggtc agctctcccc acggcaccaa cggctcaatt tcgcccatga
    37621 ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc cagctcagga tgtacgtcaa
    37681 gcggcaaatg ccagccatac aggttgatat cattcgccag caacgttttt aaacggttac
    37741 gcttcattcc gcgaatgacc ggagactctc ctttccagaa gtagccatga tgcacaatga
    37801 ccgcatccgc gcccagacgc actgcctcat cgagcaaagc ctggctggcg gtgacaccgg
    37861 taacaatttt ttgcaccgtc tctttgcctt ctacctgcaa accattcggc gcgtaatcac
    37921 taatcgccgc gctgttcagt ttttcgttaa tcagttgttc cagttcggtg tttttcatct
    37981 tcgcgccttc cgtattctga tagctacacc gttaacatag cgttacctcg cctgttcgtc
    38041 gatagccaat cttttgacat attaagaaac aaacccgacg ccaaaagacg caaattgttt
    38101 atatcatcac gcttcagcta atgtatattt atgcaatcag acagcatatt gcataaataa
    38161 tcagatctgt atcaaagttt taactttaag gaaacacaag cacgccgcgc aactattacg
    38221 tgacgttgca gtcaattgtg gaattattat gaataaacac gcatcacaac cgcgcactat
    38281 ttactatgtc gttgcgctgc aaatctggga atattttagc ttttacggca tgcgtgccct
    38341 gctgattctc tatctcacca atcaactaaa atacaacgat actcacgcct acgagttatt
    38401 tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc ggtggctttt tggcggataa
    38461 agttctcggc aatcgcatgg cggtgatgct gggggcgttg ttgatggcga tcggtcatgt
    38521 ggtgctgggt gccagtgaga tccatccgtc attcctctat ctgtccctgg cgattatcgt
    38581 ttgtggctat ggtctgttta aatccaatgt cagttgcctg ctcggcgagc tgtatgagcc
    38641 aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat gcagcgggta acgtggggtc
    38701 tattatcgca cctatcgcct gtggttacgc ccaggaagag tacagctggg cgatgggctt
    38761 tggcctggcg gcggtgggta tgatcgcggg tctggtcatt ttcttatgtg gcaatcgtca
    38821 tttcacacat acccgcggcg ttaataaaaa agtactgcgt gcaacaaact ttctcctgcc
    38881 taactgggga tggctgctgg ttctgctggt ggcaacgcct gcgctgatta ccgtactgtt
    38941 ctggaaagag tggtcggtat acgccttaat tgtcgcgact attattggcc tgggtgtact
    39001 ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgt aaggagctgg ggctgattgt
    39061 gacgctcacc ttcttcagta tgttgttctg ggccttcgca caacagggcg gcagctcaat
    39121 tagcctttat atcgaccgct tcgttaaccg cgatatgttt ggttataccg ttccgactgc
    39181 tatgttccag tcgattaatg cctt
//
LOCUS       DAJTXQ010000024        39162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000024 DAJTXQ010000000
VERSION     DAJTXQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.024"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(224..1564)
                     /gene="fadL"
                     /locus_tag="OO515_001185"
     CDS             complement(224..1564)
                     /gene="fadL"
                     /locus_tag="OO515_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6575163.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1936..2220
                     /locus_tag="OO515_001186"
     CDS             1936..2220
                     /locus_tag="OO515_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6575164.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2400..3710
                     /gene="fadI"
                     /locus_tag="OO515_001187"
     CDS             2400..3710
                     /gene="fadI"
                     /locus_tag="OO515_001187"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6575165.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3710..5854
                     /gene="fadJ"
                     /locus_tag="OO515_001188"
     CDS             3710..5854
                     /gene="fadJ"
                     /locus_tag="OO515_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6575166.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6057..6542
                     /gene="sixA"
                     /locus_tag="OO515_001189"
     CDS             6057..6542
                     /gene="sixA"
                     /locus_tag="OO515_001189"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6575167.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7226..7789
                     /locus_tag="OO515_001190"
     CDS             7226..7789
                     /locus_tag="OO515_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6575168.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7871..10513
                     /gene="yfcU"
                     /locus_tag="OO515_001191"
     CDS             7871..10513
                     /gene="yfcU"
                     /locus_tag="OO515_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6575169.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10533..11284
                     /locus_tag="OO515_001192"
                     /pseudo
     CDS             10533..11284
                     /locus_tag="OO515_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11259..11807
                     /locus_tag="OO515_001193"
     CDS             11259..11807
                     /locus_tag="OO515_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575170.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11804..12274
                     /locus_tag="OO515_001194"
     CDS             11804..12274
                     /locus_tag="OO515_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575171.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12271..12795
                     /locus_tag="OO515_001195"
     CDS             12271..12795
                     /locus_tag="OO515_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575172.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12782..13654
                     /locus_tag="OO515_001196"
     CDS             12782..13654
                     /locus_tag="OO515_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6575173.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13776..14327)
                     /gene="smrB"
                     /locus_tag="OO515_001197"
     CDS             complement(13776..14327)
                     /gene="smrB"
                     /locus_tag="OO515_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6575174.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14493..15425
                     /gene="prmB"
                     /locus_tag="OO515_001198"
     CDS             14493..15425
                     /gene="prmB"
                     /locus_tag="OO515_001198"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6575175.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15460..16545
                     /gene="aroC"
                     /locus_tag="OO515_001199"
     CDS             15460..16545
                     /gene="aroC"
                     /locus_tag="OO515_001199"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6575176.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16549..17373
                     /gene="mepA"
                     /locus_tag="OO515_001200"
     CDS             16549..17373
                     /gene="mepA"
                     /locus_tag="OO515_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6575177.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17373..18182
                     /locus_tag="OO515_001201"
     CDS             17373..18182
                     /locus_tag="OO515_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6575178.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18182..18730
                     /gene="epmC"
                     /locus_tag="OO515_001202"
     CDS             18182..18730
                     /gene="epmC"
                     /locus_tag="OO515_001202"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6575179.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18764..19042
                     /locus_tag="OO515_001203"
     CDS             18764..19042
                     /locus_tag="OO515_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6575180.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19163..21169)
                     /gene="mnmC"
                     /locus_tag="OO515_001204"
     CDS             complement(19163..21169)
                     /gene="mnmC"
                     /locus_tag="OO515_001204"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6575181.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21328..22548
                     /gene="fabB"
                     /locus_tag="OO515_001205"
     CDS             21328..22548
                     /gene="fabB"
                     /locus_tag="OO515_001205"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6575182.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22823..24001
                     /locus_tag="OO515_001206"
     CDS             22823..24001
                     /locus_tag="OO515_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575183.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(23998..24993)
                     /gene="flk"
                     /locus_tag="OO515_001207"
     CDS             complement(23998..24993)
                     /gene="flk"
                     /locus_tag="OO515_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6575184.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25092..26228
                     /gene="pdxB"
                     /locus_tag="OO515_001208"
     CDS             25092..26228
                     /gene="pdxB"
                     /locus_tag="OO515_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6575185.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26294..27307
                     /locus_tag="OO515_001209"
     CDS             26294..27307
                     /locus_tag="OO515_001209"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6575186.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27307..28119
                     /gene="truA"
                     /locus_tag="OO515_001210"
     CDS             27307..28119
                     /gene="truA"
                     /locus_tag="OO515_001210"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6575187.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28202..28861
                     /locus_tag="OO515_001211"
     CDS             28202..28861
                     /locus_tag="OO515_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6575188.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29017..29931
                     /gene="accD"
                     /locus_tag="OO515_001212"
     CDS             29017..29931
                     /gene="accD"
                     /locus_tag="OO515_001212"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6575189.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30001..31269
                     /gene="folC"
                     /locus_tag="OO515_001213"
     CDS             30001..31269
                     /gene="folC"
                     /locus_tag="OO515_001213"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6575190.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31259..31921
                     /gene="dedD"
                     /locus_tag="OO515_001214"
     CDS             31259..31921
                     /gene="dedD"
                     /locus_tag="OO515_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6575191.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32180..32668
                     /gene="cvpA"
                     /locus_tag="OO515_001215"
     CDS             32180..32668
                     /gene="cvpA"
                     /locus_tag="OO515_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6575192.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32705..34222
                     /gene="purF"
                     /locus_tag="OO515_001216"
     CDS             32705..34222
                     /gene="purF"
                     /locus_tag="OO515_001216"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6575193.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34317..34886
                     /gene="ubiX"
                     /locus_tag="OO515_001217"
     CDS             34317..34886
                     /gene="ubiX"
                     /locus_tag="OO515_001217"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6575194.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35152..35934
                     /gene="argT"
                     /locus_tag="OO515_001218"
     CDS             35152..35934
                     /gene="argT"
                     /locus_tag="OO515_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6575195.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36155..36937
                     /gene="hisJ"
                     /locus_tag="OO515_001219"
     CDS             36155..36937
                     /gene="hisJ"
                     /locus_tag="OO515_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6575196.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37027..37713
                     /gene="hisQ"
                     /locus_tag="OO515_001220"
     CDS             37027..37713
                     /gene="hisQ"
                     /locus_tag="OO515_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6575197.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37710..38426
                     /locus_tag="OO515_001221"
     CDS             37710..38426
                     /locus_tag="OO515_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6575198.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38434..>39162
                     /gene="hisP"
                     /locus_tag="OO515_001222"
     CDS             38434..>39162
                     /gene="hisP"
                     /locus_tag="OO515_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6575199.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNP"
BASE COUNT         9269 a         9600 c        10540 g         9753 t
ORIGIN      
        1 aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat atgcctttcc
       61 cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc caacaggctg
      121 tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg ggggagttaa
      181 agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac gcgtagttaa
      241 agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg ccttcgttaa
      301 ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca tctttattaa
      361 atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg gagatagaac
      421 gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag gtccagttat
      481 catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag ccttcatgtt
      541 tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac tgactccagc
      601 tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta taacctgaca
      661 cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg ccacctgtcg
      721 cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg ctgtagttac
      781 ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta tttttatcca
      841 gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc agatgagcga
      901 ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct tgaggagttt
      961 gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg cctgcgaaac
     1021 gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc catgcattat
     1081 ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt gtacccccga
     1141 cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta gaggtaatag
     1201 aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc ggaacccatg
     1261 ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt ccgctgatat
     1321 tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca aacatggtaa
     1381 tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct tcccctgaat
     1441 aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc gaccaggcct
     1501 gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg gttttctggc
     1561 tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc tgtaacagga
     1621 gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga gagtataggt
     1681 cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga tctaagaaac
     1741 gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga attttgtttt
     1801 agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta acaactggag
     1861 cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg ggtgcacata
     1921 cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg ctgctgtatg
     1981 gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg cgtgtttgca
     2041 aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg tagcgtggaa
     2101 tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt gcgcctggat
     2161 atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg tctgcgttaa
     2221 ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct tttctttctc
     2281 ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta aatgtaaaaa
     2341 aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag gggagtgtta
     2401 tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt gttagcggtt
     2461 tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg gttgatttag
     2521 ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa gtgattgaac
     2581 agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg cgtgaaattg
     2641 ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc gcttgcgcta
     2701 ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact atccgagcgg
     2761 ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt aaaaaactgg
     2821 cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg aaactcttct
     2881 ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa tattctactg
     2941 gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc acccgagaac
     3001 agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg tcagacggaa
     3061 aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg cttgtcgaag
     3121 acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc ccggcgtttg
     3181 atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat ggcgcggcag
     3241 cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg ctggggtatc
     3301 tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc ggtccagcct
     3361 ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg acattgatcg
     3421 atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg ggtagtgaac
     3481 gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac gatagcaaat
     3541 ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc ggcgcgcgga
     3601 tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt ttagttaccg
     3661 cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa tggaaatggc
     3721 atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca tcgatgtacc
     3781 ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg ccattattaa
     3841 gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga aaccggacaa
     3901 ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc aagaagcgga
     3961 agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc ccattccggt
     4021 tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc tggcgtgcca
     4081 cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag tacaacttgg
     4141 attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg tcagtacagc
     4201 attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa agctggggct
     4261 ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc tggcgaagaa
     4321 ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg ggccgttagg
     4381 tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc aaggcaatta
     4441 tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg gcaccagcag
     4501 cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc aatcgcaagc
     4561 tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta gtgatgcacc
     4621 gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg gcggtattgc
     4681 ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca acccgcaggg
     4741 cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc gccgtcgtca
     4801 tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga cggactatcg
     4861 cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg aattaaaaca
     4921 acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg cttcgaacac
     4981 gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc aagttattgg
     5041 cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc ctcatgcggg
     5101 tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg gcaaaacgcc
     5161 cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc cttacattaa
     5221 tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg ccgcgctagt
     5281 gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa tcgacaccgg
     5341 gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg cgcctgcaaa
     5401 tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc ggggtttcta
     5461 tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca tttacccgct
     5521 cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac ggtgtgtgat
     5581 gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta gcgtgcgtga
     5641 cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg gaccgttccg
     5701 ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac tggccacgca
     5761 gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc gtggggaaag
     5821 tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata tgaatccgcg
     5881 ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta cgccattgag
     5941 gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca ccgttttctt
     6001 tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg tgcaatatgc
     6061 aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat tcggttcgtc
     6121 ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg aaaggtcaaa
     6181 aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa acactggaag
     6241 aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag ttaacgccct
     6301 gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa ggtgttgctt
     6361 cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag ttatgtccgg
     6421 gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt gatgaaagcg
     6481 gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca aaagctatct
     6541 gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc tcatcaggca
     6601 gcggatatct gtcaatctca ctactatcga agactctccc catctataaa taaaacactt
     6661 tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca aagttacatg
     6721 ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga taatagagaa
     6781 aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct ggctatagtg
     6841 tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt tcattgtttg
     6901 ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa atacttattc
     6961 cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt aattaaatct
     7021 acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca ctatgcggcc
     7081 ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg ccgtattcaa
     7141 tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt tatattttta
     7201 attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta ttgctgcggc
     7261 aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca acggtactgc
     7321 acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg atcacaaact
     7381 ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca ccaccacgcc
     7441 gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa caatgaccac
     7501 tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca ttttcaatac
     7561 cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc tgggtacctc
     7621 ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta ccaataaagg
     7681 caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg atgcgccaga
     7741 tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag gcataccccc
     7801 atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat aaaaacggat
     7861 agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt ggtgcattgc
     7921 gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc agtttgattc
     7981 ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt ccagccaggg
     8041 atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc cattggcgga
     8101 agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct atgcttgtct
     8161 gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa atctgcaatg
     8221 gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa ttaaggctga
     8281 tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat atacttatcc
     8341 cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg cggactacag
     8401 catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg agatcagcgg
     8461 caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact ggcagactaa
     8521 ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa ctcaaaaaaa
     8581 atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag ccaaactggc
     8641 gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg ttggtggcag
     8701 tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc cagacatttc
     8761 cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg tgatttacga
     8821 aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg tctccggtac
     8881 gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca tcagcaccgc
     8941 ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga tgggccgtcc
     9001 gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag cttcctgggg
     9061 gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact atcagtctgc
     9121 ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg atgttactca
     9181 ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg acggtaactc
     9241 cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta cttttgctgg
     9301 atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg ccagtgacag
     9361 cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata accagaactt
     9421 ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact gggatcgcga
     9481 ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca gtattcgtaa
     9541 tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg acaaaggcat
     9601 gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata acggtaacta
     9661 tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg acgcgactca
     9721 ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct attacagcca
     9781 tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc aatacacctc
     9841 tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg cacttcaccg
     9901 tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg ccgatgttcc
     9961 ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg tcgtttctga
    10021 cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc cggaaaacgc
    10081 cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg gctaccgcaa
    10141 atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag acggcagcca
    10201 tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc ttgtcgatga
    10261 tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg tgttctggag
    10321 tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc tgtttaacgg
    10381 cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg atgacataaa
    10441 gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg tttctgtttc
    10501 agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc tctgccaaat
    10561 tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc aggcttctgt
    10621 aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca gcgtgacgtt
    10681 gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag atgaaaaggg
    10741 taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg attcaatgat
    10801 gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg ccgatcgtga
    10861 aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag ccaacacgct
    10921 gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc tggagaacgt
    10981 cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc aggaatttac
    11041 cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac agaaaaatgg
    11101 caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg caccgctgaa
    11161 tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg ctcggatgcc
    11221 gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga gccgcaaagg
    11281 ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta actgggttac
    11341 tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta agtatgaatt
    11401 tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca gttgagtttg
    11461 gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt gactactcgc
    11521 ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg acaacggtcg
    11581 tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt cgggttcaga
    11641 aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca tttaattttt
    11701 cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca acgttagcgg
    11761 cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac ggatctcgct
    11821 tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc acggttattt
    11881 agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa cttttcagga
    11941 tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt attccattac
    12001 ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga gtggcacact
    12061 aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca ttcagctcaa
    12121 acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa ccgatttacc
    12181 ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg cggactttga
    12241 ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt gttttttatt
    12301 agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt gcactttacc
    12361 ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt ggcagaagta
    12421 cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga gcgagtgcca
    12481 ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa agtaacgttg
    12541 acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc gtcatcagca
    12601 tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat caacaataca
    12661 acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa tacctggtta
    12721 caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt gacggcaacc
    12781 tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa tgctgctcgt
    12841 tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga gtgcgagttt
    12901 tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag cgagcagtgt
    12961 tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg attcttttgc
    13021 gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag ctccaacttt
    13081 ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag atacgaaaat
    13141 ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt atggttgccc
    13201 ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct atacggcccc
    13261 tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata tctcctggag
    13321 tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt caacagtgac
    13381 caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca gtaatttttc
    13441 tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa cctttacctc
    13501 ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg taacggataa
    13561 agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga ttgattcgac
    13621 gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct gatgcgtctt
    13681 atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta ggccggataa
    13741 ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg cggcaaccac
    13801 tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt cggtgcctgg
    13861 tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg cttcaaaata
    13921 tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg acaggcggca
    13981 atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa atccaaaaac
    14041 aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct gacatccggg
    14101 cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc ggagaaataa
    14161 tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct gattttttta
    14221 cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc catcaactgg
    14281 cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta tctgtctcta
    14341 tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt aaacgtattt
    14401 ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa actagccgcc
    14461 gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt gatgaagcag
    14521 taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc ttcagcgcgg
    14581 caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta cagctggtgt
    14641 taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg cgtctgacct
    14701 ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag cgcattccgg
    14761 tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc gatgaacgcg
    14821 tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc ggactcatta
    14881 gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc gccattgcct
    14941 gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc gacgcgctgg
    15001 cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt ccgattcgtt
    15061 ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact aacccgccgt
    15121 atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag ccggaactgg
    15181 gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga aacgcggcag
    15241 attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg gtacatctta
    15301 tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc ggcgatggtg
    15361 tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg atttataaag
    15421 attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa cacaattgga
    15481 caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg ctgcatcgtc
    15541 gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga cctcgaccgt
    15601 cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca ggtcaaaatt
    15661 ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt gatcgaaaac
    15721 actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc aggccatgct
    15781 gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg acgttcttcc
    15841 gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata tctcgccgag
    15901 aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc gctggaaatc
    15961 aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga caaaatcgac
    16021 gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg cgcgaaagtc
    16081 accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga ccgcctggat
    16141 gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga aattggcgac
    16201 ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac caaagacggt
    16261 ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca gcaaatcatt
    16321 gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac tattaaccgc
    16381 tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt cgggatccgt
    16441 gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct gttacggcag
    16501 cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat gaacaaaacc
    16561 gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac gccgtggcaa
    16621 aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc taatggctgt
    16681 attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat gcgtaccgat
    16741 cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct gagtagccag
    16801 gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc cgctggtggg
    16861 cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat ctttctgcaa
    16921 ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact ggacttagtt
    16981 tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt cagcttgatc
    17041 aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc gattaaacaa
    17101 caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg accctggttc
    17161 cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct ggagtgtgaa
    17221 gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag ctggtttgca
    17281 cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt gccgccttcc
    17341 tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc ctgtttatgg
    17401 tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga tttatcgact
    17461 cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg ggaatgtctc
    17521 ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt tccgccacta
    17581 tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat attgccatga
    17641 cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct gatgtcttac
    17701 ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg atgccaaagc
    17761 tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta attgccggtg
    17821 gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt tacgcgctgg
    17881 ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc aaattactca
    17941 acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa gtgatttggg
    18001 cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt tcacggctgg
    18061 tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg gcggtgatga
    18121 gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg gggatgaact
    18181 aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct gctttgccga
    18241 tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc ctgctgatgc
    18301 ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg caattcatga
    18361 gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt tcggctactg
    18421 gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg ttgaagtgaa
    18481 gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta acgtcagttg
    18541 cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc gggtacatgc
    18601 gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct tcattaaagc
    18661 actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt ggccggaagc
    18721 gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg cggagtttga
    18781 atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg atgagttgtt
    18841 tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg aaagtggtga
    18901 tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa aagccattgg
    18961 tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg aaaacctgtt
    19021 tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga tgcgacgctt
    19081 atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc acgccgcatc
    19141 cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc aacaatttcc
    19201 gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc ggaatcggtt
    19261 cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac aaaccgcgag
    19321 aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc gcctcatctt
    19381 tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga acattgccta
    19441 ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct ttatcactga
    19501 catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc tggcgattct
    19561 gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa ctggcaccaa
    19621 tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag cacagcacct
    19681 gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg gcaaccgaat
    19741 acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc gccagtacca
    19801 ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag tcatccttac
    19861 gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt tgcgccagtt
    19921 ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga taagtaatgc
    19981 cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca acggctacag
    20041 ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg cttttctcat
    20101 cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt aattggtcgt
    20161 aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc tcatcgtgtt
    20221 tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc gcgggggctt
    20281 catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc gacaacaacg
    20341 cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc gtacgattaa
    20401 accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa agcatttccc
    20461 gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc aaaccgcggc
    20521 ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc aaccttgcca
    20581 tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca aagccgtcca
    20641 gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc agttcgttaa
    20701 tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa cgatggcaac
    20761 cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc agttccggcc
    20821 agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca aaactaatga
    20881 aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca aatgcctgcc
    20941 atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca aacagaggat
    21001 gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca taacgcgtct
    21061 cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa acaggtgtac
    21121 cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg taagttactc
    21181 gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa tttctctatt
    21241 aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt cgaaacttac
    21301 tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg cctgggcatt
    21361 gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga aggacgttca
    21421 gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt ctggggcaac
    21481 gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat gagcgacgca
    21541 tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct ttctccggaa
    21601 gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg ttccccgcgt
    21661 ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc ggttggcccg
    21721 tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac gccgtttaaa
    21781 attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca ctgtatcggt
    21841 aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg cggcggcgaa
    21901 gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc tactaaatac
    21961 aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg tttcgttatc
    22021 gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc gcgtggtgct
    22081 cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga catggttgct
    22141 ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt tgatacccca
    22201 atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa agagctggca
    22261 gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa agccatgacc
    22321 ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct gatgctggaa
    22381 cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc tgcgggtctg
    22441 aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa cagcttcggc
    22501 ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt cgcagtaggc
    22561 cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc aatgcggcct
    22621 ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg acaatcgcca
    22681 ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt acgcgttaaa
    22741 tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac tcctcgcctt
    22801 gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac gatcttctgc
    22861 caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga ccgtagggct
    22921 gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca ataccatggt
    22981 cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct atgccgggcg
    23041 actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag cttgtggtct
    23101 ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt tcaaatttgc
    23161 cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac tgacaggcgc
    23221 tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga tgtcatggaa
    23281 tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt tgattcatag
    23341 ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac tggcgtgggc
    23401 ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat cgctgtggag
    23461 cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg ttggttttgc
    23521 ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga tggcgggctt
    23581 tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg gctggatgcc
    23641 ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa cggtgggctt
    23701 gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg cgttaaccgg
    23761 agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac gcgtcccctc
    23821 acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg ccctcggcgt
    23881 ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg tatttcttgc
    23941 cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc gtcggggcta
    24001 acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca gcgcgggacg
    24061 cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca gcggttgcat
    24121 atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa tatcctcggc
    24181 ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga tatacgccga
    24241 taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta gtgattccag
    24301 cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca ccagatggtt
    24361 aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca gcatcgattg
    24421 ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca gctgttttag
    24481 cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct gtggttgcgg
    24541 aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct ccggtgagag
    24601 ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg cctgacgatt
    24661 attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag aatggctttt
    24721 ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt gacgcgaaag
    24781 cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca gttctgctct
    24841 ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag tacgttgctg
    24901 actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac ctggtggttg
    24961 cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct gaaatcttcg
    25021 caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg tctcatacag
    25081 gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc gacttattta
    25141 gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct caactggcag
    25201 acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg ctggcaggaa
    25261 aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat gaagcatggt
    25321 tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt gcggtggtgg
    25381 aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca ctgcacgacc
    25441 gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg cgactggaag
    25501 cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg gatgagggtg
    25561 atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc catacgccac
    25621 tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg atccgtagcc
    25681 tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat aatactgcgt
    25741 tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc tgggaaggcg
    25801 aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg catatcgcag
    25861 gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc tatagcaagt
    25921 ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca gagtttggtc
    25981 gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg catttggtgt
    26041 atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg ggtgagttcg
    26101 ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat gtaatttgtg
    26161 atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt catcatccgg
    26221 cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt tgctatttct
    26281 ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa ctggcgctgt
    26341 gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg aaatttatgc
    26401 actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa caatcactgt
    26461 gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg tcgcaggcaa
    26521 agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg tgatcgacag
    26581 cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag taaacccgtt
    26641 tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc tgaccagcca
    26701 gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta tcagtgttac
    26761 cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag cggggcagag
    26821 tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc gtcaactggc
    26881 gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag aacgtcgtat
    26941 cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg caagcgtcgt
    27001 ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac tgcgtccgct
    27061 ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc tctctgaaga
    27121 gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt ctgttggctg
    27181 cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg ccgataacgt
    27241 tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc aggagtatct
    27301 gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg cattgagtac
    27361 gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt gcaggagaag
    27421 ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg cgccgggcgt
    27481 actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac cgcgcagcgc
    27541 aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat cgccgtgcgt
    27601 tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc tcgccgttat
    27661 cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg ggtaacccat
    27721 ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt gctgggcgag
    27781 aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg gcgcaacgtt
    27841 atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa agcgaatgcc
    27901 tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg tgcccacaac
    27961 cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct ggcggcagca
    28021 acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg gtatgatctt
    28081 ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg ctcggaacat
    28141 ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg cataggcaaa
    28201 tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc atctggcaga
    28261 gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc tgttctgcga
    28321 aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg ttgctggtgc
    28381 attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc ttatgttgat
    28441 tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg gtgacaaact
    28501 gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga ctcatcagtt
    28561 ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga tcgtcagaac
    28621 gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg ccgcttataa
    28681 cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt tcttcggcac
    28741 gctccccttc attcagagca accttaagct gatgattgtt ggcattattt ttgtttcaat
    28801 attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg ccgcaaaata
    28861 aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt tatgttttaa
    28921 tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc aaagataaaa
    28981 ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga acgaattaaa
    29041 agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac taagtgtgat
    29101 agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt ctgtccgaag
    29161 tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt agatgaagga
    29221 agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt tcgtgactcc
    29281 aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa agatgcgctg
    29341 gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt cgagttcgcc
    29401 tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc cgttgagcag
    29461 gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc acgtatgcag
    29521 gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc aaaaatgcag
    29581 gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg tgtttctgca
    29641 agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat tggctttgcc
    29701 ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt ccagcgcagt
    29761 gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga aatgcgcctg
    29821 aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc tgaagcgccg
    29881 cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg ataactgata
    29941 agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc agcggatacc
    30001 atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct ttcttatctg
    30061 gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca ggtcgcggcg
    30121 cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac gaatggcaaa
    30181 ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa agtgggcgtc
    30241 tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg gcaggaattg
    30301 ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg tgatatttcc
    30361 ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca ggcacaactt
    30421 gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa tattgtcgat
    30481 gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct gggaccagat
    30541 cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc ggcaattgtc
    30601 ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg tgcactgtta
    30661 caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc gtttagcgat
    30721 gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa tgccgcaaca
    30781 gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat tcgcgacggg
    30841 attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc acgcgttatt
    30901 tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat gaaagcgcta
    30961 ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga tattgccgga
    31021 actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact ggaagggccg
    31081 cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc atttgatagc
    31141 gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac cgtgctggtg
    31201 tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag gagaagcggt
    31261 ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc tgggggtgat
    31321 tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt ttgcggctat
    31381 cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag ccgccaccca
    31441 ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag gtgatgccgc
    31501 agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg aaccggaacc
    31561 tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga aggttgaagc
    31621 accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag cgccaacggg
    31681 taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga atgagattgt
    31741 cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc cagtgcaggg
    31801 taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga aaggttcgct
    31861 gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata cgccgaatta
    31921 atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag tgttagaacc
    31981 gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc cgcaaaggca
    32041 taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc gtgggaagga
    32101 aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg atgacagggc
    32161 gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg tgattgcttt
    32221 ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc tggtgacatg
    32281 gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct ggtttacggg
    32341 ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta tcgctaccct
    32401 gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa cggggttgtc
    32461 aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt tgattgttgc
    32521 cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag actggagcaa
    32581 atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt atctgcaaag
    32641 ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac gaggaaaaag
    32701 acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt cgatttatga
    32761 tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca ccatagatgc
    32821 caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat ttgaagctcg
    32881 ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc ctacggctgg
    32941 cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg gcattacgct
    33001 tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt ttgaagaaaa
    33061 acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct tcgctagcga
    33121 gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg ccattgctgc
    33181 cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg gccacggtat
    33241 ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac gtgatattga
    33301 cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata cgctgggctt
    33361 tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag aagggcagtt
    33421 gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg agtatgtata
    33481 ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc gtgtgaatat
    33541 gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata tcgacgtggt
    33601 gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta ttctgggcaa
    33661 accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta tcatgccggg
    33721 ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg ccgagttccg
    33781 cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt ctgagcagat
    33841 tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg cggcaccgga
    33901 aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac tgatcgctca
    33961 cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga ttttccagga
    34021 tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc agtttgaatg
    34081 ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc tcgatttcct
    34141 cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag tggaaaatct
    34201 cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca ccctgacttg
    34261 caactcccgc cgaaatacta caaagtctgc ctgcaagtct gacagggcaa ctatttatga
    34321 aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg cgcttattac
    34381 aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag gcggcgcgcc
    34441 agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta gctgatgtca
    34501 cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag acgctgggga
    34561 tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc tatactgatg
    34621 gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg gtgctctgcg
    34681 tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg gcagaaatcg
    34741 gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc cttgatgatg
    34801 tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt cctgaagatc
    34861 tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg caattttgca
    34921 accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct gcgattcaac
    34981 cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta acatatttaa
    35041 cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga gcctgcaaac
    35101 acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg tatgaagaag
    35161 tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag ctatgcggcg
    35221 ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc gtcgaaagat
    35281 gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg caaacggatg
    35341 caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc actgaaagcg
    35401 aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca gcaggagatt
    35461 gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa aggttcaccg
    35521 attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca gggatcaacc
    35581 caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt ggcctatgcc
    35641 aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc gttacaagat
    35701 gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt cgcctttgct
    35761 ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg gctacgtaaa
    35821 gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg tcaggacggc
    35881 acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga ctgatacgtc
    35941 gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt gttgcactat
    36001 cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg tctaacgggg
    36061 cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa acctgtaaat
    36121 attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc tatcgctctc
    36181 tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc aaaacatccg
    36241 catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag gcgaactggt
    36301 tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc aatgtacgtt
    36361 tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga ttgacgccat
    36421 catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca ccgacaaact
    36481 gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc cgacagtcga
    36541 gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga cgttcggtaa
    36601 tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg acaacattta
    36661 ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg ctgccagcga
    36721 aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt ctgttaaaga
    36781 tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata acgaactgcg
    36841 cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg agaaattagc
    36901 gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga aagaaagccc
    36961 gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca cgacaggaca
    37021 ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt cacgctggag
    37081 ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc tggcggtaag
    37141 ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct gattcgtggc
    37201 gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc gctaaacacg
    37261 gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc tggtattatc
    37321 actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc ttttatggca
    37381 gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg gcaagtgttt
    37441 cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg caacaactgg
    37501 caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga tgtggtcaaa
    37561 gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat cgtctgtggc
    37621 gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct tgagcgccgc
    37681 tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa tactggaaac
    37741 cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg tggctgctta
    37801 ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt cgtgtctcca
    37861 gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc ggtacgccgc
    37921 tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt gttaagggaa
    37981 ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg gcgctgacgc
    38041 ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg gttccgcatg
    38101 gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat cgttgcatta
    38161 ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg atcctgatgc
    38221 tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa atagcccgcg
    38281 atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg gtgctctatt
    38341 taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc tggttgcagc
    38401 atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt aaacgttatc
    38461 gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact gcaagcgaat
    38521 gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac ctttctgcgc
    38581 tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg ccagacgatc
    38641 aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca actgcgctta
    38701 ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca tatgacggtg
    38761 ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca ggaagcgcgc
    38821 gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca ggggaaatat
    38881 ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc gctggcgatg
    38941 gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga actggtaggc
    39001 gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt ggtagtgact
    39061 cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca tcaggggaaa
    39121 atagaagaag agggcgcgcc ggagcagtta tttggcaacc cg
//
LOCUS       DAJTXQ010000025        38853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000025 DAJTXQ010000000
VERSION     DAJTXQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.025"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            148..546
                     /gene="fadM"
                     /locus_tag="OO515_001223"
     CDS             148..546
                     /gene="fadM"
                     /locus_tag="OO515_001223"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6575200.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(598..1293)
                     /gene="queC"
                     /locus_tag="OO515_001224"
     CDS             complement(598..1293)
                     /gene="queC"
                     /locus_tag="OO515_001224"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6575201.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1358..3058)
                     /locus_tag="OO515_001225"
     CDS             complement(1358..3058)
                     /locus_tag="OO515_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6575202.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3158..3976
                     /gene="cof"
                     /locus_tag="OO515_001226"
     CDS             3158..3976
                     /gene="cof"
                     /locus_tag="OO515_001226"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6575203.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4128..4586
                     /gene="decR"
                     /locus_tag="OO515_001227"
     CDS             4128..4586
                     /gene="decR"
                     /locus_tag="OO515_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6575204.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4616..6388
                     /locus_tag="OO515_001228"
     CDS             4616..6388
                     /locus_tag="OO515_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6575205.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6381..8162
                     /locus_tag="OO515_001229"
     CDS             6381..8162
                     /locus_tag="OO515_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6575206.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8343..8681
                     /gene="glnK"
                     /locus_tag="OO515_001230"
     CDS             8343..8681
                     /gene="glnK"
                     /locus_tag="OO515_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6575207.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8711..9997
                     /gene="amtB"
                     /locus_tag="OO515_001231"
     CDS             8711..9997
                     /gene="amtB"
                     /locus_tag="OO515_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6575208.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10046..10906)
                     /gene="tesB"
                     /locus_tag="OO515_001232"
     CDS             complement(10046..10906)
                     /gene="tesB"
                     /locus_tag="OO515_001232"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6575209.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11124..11696
                     /locus_tag="OO515_001233"
     CDS             11124..11696
                     /locus_tag="OO515_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6575210.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11727..12038)
                     /locus_tag="OO515_001234"
     CDS             complement(11727..12038)
                     /locus_tag="OO515_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6575211.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12201..12297
                     /gene="ffs"
                     /locus_tag="OO515_001235"
     ncRNA           12201..12297
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO515_001235"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12417..12770
                     /locus_tag="OO515_001236"
     CDS             12417..12770
                     /locus_tag="OO515_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6575212.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(12812..14360)
                     /gene="pdeB"
                     /locus_tag="OO515_001237"
                     /pseudo
     CDS             complement(12812..14360)
                     /gene="pdeB"
                     /locus_tag="OO515_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14524..14994)
                     /locus_tag="OO515_001238"
     CDS             complement(14524..14994)
                     /locus_tag="OO515_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6575213.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15110..15661)
                     /gene="maa"
                     /locus_tag="OO515_001239"
     CDS             complement(15110..15661)
                     /gene="maa"
                     /locus_tag="OO515_001239"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6575214.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(15832..16050)
                     /gene="hha"
                     /locus_tag="OO515_001240"
     CDS             complement(15832..16050)
                     /gene="hha"
                     /locus_tag="OO515_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6575215.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16076..16450)
                     /gene="tomB"
                     /locus_tag="OO515_001241"
     CDS             complement(16076..16450)
                     /gene="tomB"
                     /locus_tag="OO515_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6575216.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(16996..20145)
                     /locus_tag="OO515_001242"
     CDS             complement(16996..20145)
                     /locus_tag="OO515_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6575217.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20168..21361)
                     /gene="acrA"
                     /locus_tag="OO515_001243"
     CDS             complement(20168..21361)
                     /gene="acrA"
                     /locus_tag="OO515_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6575218.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21503..22150
                     /gene="acrR"
                     /locus_tag="OO515_001244"
     CDS             21503..22150
                     /gene="acrR"
                     /locus_tag="OO515_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6575219.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22278..25640
                     /gene="mscK"
                     /locus_tag="OO515_001245"
     CDS             22278..25640
                     /gene="mscK"
                     /locus_tag="OO515_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6575220.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(25852..26013)
                     /gene="rsmS"
                     /locus_tag="OO515_001246"
     CDS             complement(25852..26013)
                     /gene="rsmS"
                     /locus_tag="OO515_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6575221.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26027..26554)
                     /gene="priC"
                     /locus_tag="OO515_001247"
     CDS             complement(26027..26554)
                     /gene="priC"
                     /locus_tag="OO515_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6575222.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26624..27001
                     /locus_tag="OO515_001248"
     CDS             26624..27001
                     /locus_tag="OO515_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6575223.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27154..27705
                     /gene="apt"
                     /locus_tag="OO515_001249"
     CDS             27154..27705
                     /gene="apt"
                     /locus_tag="OO515_001249"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6575224.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            27834..29759
                     /gene="dnaX"
                     /locus_tag="OO515_001250"
     CDS             27834..29759
                     /gene="dnaX"
                     /locus_tag="OO515_001250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6575225.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29092..29156
                     /gene="dnaX"
                     /locus_tag="OO515_001250"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            29812..30141
                     /locus_tag="OO515_001251"
     CDS             29812..30141
                     /locus_tag="OO515_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6575226.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30141..30746
                     /gene="recR"
                     /locus_tag="OO515_001252"
     CDS             30141..30746
                     /gene="recR"
                     /locus_tag="OO515_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6575227.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            30856..32730
                     /gene="htpG"
                     /locus_tag="OO515_001253"
     CDS             30856..32730
                     /gene="htpG"
                     /locus_tag="OO515_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6575228.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            32911..33555
                     /gene="adk"
                     /locus_tag="OO515_001254"
     CDS             32911..33555
                     /gene="adk"
                     /locus_tag="OO515_001254"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6575229.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33687..34649
                     /gene="hemH"
                     /locus_tag="OO515_001255"
     CDS             33687..34649
                     /gene="hemH"
                     /locus_tag="OO515_001255"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6575230.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34646..35605)
                     /gene="aes"
                     /locus_tag="OO515_001256"
     CDS             complement(34646..35605)
                     /gene="aes"
                     /locus_tag="OO515_001256"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6575231.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35757..37061
                     /gene="gsk"
                     /locus_tag="OO515_001257"
     CDS             35757..37061
                     /gene="gsk"
                     /locus_tag="OO515_001257"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6575232.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37194..>38853)
                     /locus_tag="OO515_001258"
     CDS             complement(37194..>38853)
                     /locus_tag="OO515_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6575233.1"
                     /translation="LITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAGPFTPGF
                     VADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLLGMALSA
                     VLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLVMVLTLV
                     LLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARSAATGSR
                     ELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLPLRDAFA
                     VLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTALTIAAS
                     LAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLAKTETLE
                     EQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSRTRVDEL
                     RERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPDIEIIAR
                     AHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9354 a         9944 c        10267 g         9288 t
ORIGIN      
        1 ccggggatgg gcaattcgct ggtggaacgt aatctggcgg tattaaccct gtaattaatt
       61 tgcatagtgg caattttttg ccagactgaa gaggtcatac cagttatgac ctctgtactt
      121 ataacaacaa cgtaaggtta ttgcgctatg caaacacaaa tcaaagttcg tggatatcat
      181 ctcgacgttt accagcacgt caacaacgcc cgctaccttg aatttctcga agaagcccgc
      241 tgggatgggt tggaaaatag cgacagtttt cagtggatga cggcccataa catcgccttc
      301 gtcgtggtca atatcaatat taactatcgt cgcccagcgg tattaagcga cctgttaact
      361 attaccagtc agttgcagca attaaacggt aaaagcggca tcttaagcca ggtcattaca
      421 ctggagccgg aagggcaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt
      481 aaaacgcaga aagcattagc tctggaaggg gaattgcgcg aaaagctgga gcagatggtt
      541 aagtaaacgt tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta
      601 cctcaacccg gttttctgct tcatcgctgc catcaccgtc ggtttatcgg ccagataatg
      661 attcaaaccg ttggcgcgta aattacatgc cgcacaatga ccgcaaccgt cgcctttaat
      721 gccgttatag caggtcaacg tttcgttacg gactaaatcc agtttgccgt aataatctgc
      781 cagcgcccag gtttccgctt tatcaatcca catcagcggc gtttcaaaac gaatatcttt
      841 cgccatgccc aaactgacgg catggtttag tgctttcaca aactcatcgc ggcaatccgg
      901 gtagccggag aaatccgttt cacagacgcc ggtaattacg gcttctgctt ttacctgata
      961 cgcatatatt gccgccagcg tcaggaacaa aatattacgc ccagggacaa acgtattcgg
     1021 gatgccatcg gcttcaggtt cataatcagg caccggaatg ctgtcacgtg tcaggctgct
     1081 gaccgccagc tcgttgagca gcgttacatc aagaacctta tgcgcgcgtg ctcccagttt
     1141 cagcgccagt tcgcgtgcca cgtcgatttc tgcgcgatgt cgctgaccgt aatcgaacgt
     1201 cacgcaatgg acttcatcat attgttgtaa tgcctgcacc agacaggtgg tggaatcctg
     1261 gcctccactg aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc
     1321 gttatggtaa cgcctgcgat taacccggac cagctattca ttgcgacggc gcgggaagcc
     1381 aggcttcggt aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag
     1441 gggcactaat gcgatagtga tagttgaaca gcggcgtcag cgtcgcgtct gccattaact
     1501 ggctaaaaac cgctttcagg gcattaaatc ggttttcctc atcaggcatt acttgcaccg
     1561 cgtccagcgt cgattgcaga tgtgcatatg ctggagcgtc gaaaacatgt ggccatagtg
     1621 gatcgcaacg tagccattgc tccagagtat attccggtgc ttcgccaatt aatctgtcgc
     1681 ccatcatgag gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa
     1741 aaataattgt gagttcacag ccttccgctg ccagtgtcgc ctgtaggcgt tctgccatgg
     1801 tatgaagttc tatcggtagg tgataaacca gcgtcaaggt tttcggtagt ttgacttcat
     1861 ccggtacttg ccagtgcgga atagtccagc ctggcagtaa tgcatgactg gcggtgatca
     1921 ggttttctcc gacttctaac gtttgtaata aaccggattg atgaataatg gagatcactt
     1981 ttcgcgcctg ccagagggag agtcggggac ttttgcgcaa cgttaaatag caaaaaccta
     2041 aactgatgcc actactgacc tggctgaccc gttgcagctc ctccggtttg ccgatggtga
     2101 tttgcacggg atgccgacaa ctggttccca aatctttttc gaaaagcggc ggagttatcc
     2161 agtactcaac cgctttaagc agcggatgac gcaaatggta ataatcatgg ctctccaggc
     2221 gcaccagctc cgctgtgaat tgtgttaatc gaaaaggacc agtgccgatc agtgggaatt
     2281 gcggatgcgc cagatggctg caatagctcg ccagccggtg cgcaagccag taatcggggc
     2341 gatgtaaaaa gaaggtcaga cactgcggat gggtgacttc aatacgcttc acgctaataa
     2401 ataattgatc cagtgctggc agttgtaaca gcatcaataa tcgctggtgt aagtgtgagg
     2461 cttttactgc atcgccgtta tgccagtgta gggttgaacg aagataaaag tcccagcgta
     2521 acccgtcagt agaggtttcc ctgtgatgcg ctaaatcgcc aatcgggcgc tgagtattat
     2581 tatcgaagcg ggtcaggccg gaaaatatct gcccggcgag atgctgctcg gcacggccgg
     2641 gcaaaaagcc tggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggtg
     2701 tatcgttttg ccattgtccg cccataaacg gctgtaacag agtgcgcagc tcacctgggg
     2761 ccagttgcgc cagctccagc acatcttgct gctttccggt ttccagtgcc tgttccatca
     2821 tcgcattgcg tagcgattcc ggcgtgacca gaaagcgtaa ttgtccgcgc tttccgcgtc
     2881 ctgactgcgc ctgccactcc agccatcccg cctcctgtgc ctgacgcaac agcgtacgaa
     2941 catggcgttc gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct
     3001 ttccggctga aggttgccac agacgttgat actggttaag acggttgagc aatcgcatat
     3061 aaacccggaa caatattatt taactattca ctattacttc cgtatatatc aggtgatact
     3121 caatctccat taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga
     3181 tatggatggc actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt
     3241 ggcgcgactg cgtgaacgcg acattaccct cacttttgcc acggggcgtc atgcgctgga
     3301 gatgcagcat attctcgggg cgctatcgct ggatgcatat ttgattaccg gcaacggaac
     3361 gcgcgtgcat tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc
     3421 ggagctggtg ctgtatcagc aatgggatac ccgagccagc atgcatatct tcaatgacga
     3481 cggttggttt accgggaaag agatccctgc gttgttgcag gcatttgtct atagcggttt
     3541 tcgttatcag ataatcgatg tcaaaaaaat gccactcggc agcgtcacca agatctgctt
     3601 ctgtggcgat cacgacgatc ttacacgctt gcagatccag ctatacgaag cattaggcga
     3661 gcgtgcacat ttgtgttttt ccgccacaga ttgcctcgaa gtgctgccgg tgggctgcaa
     3721 taaaggcgct gcattgacgg tgctgaccca acatttaggt ttatcgttgc gcgattgcat
     3781 ggcctttggt gatgcgatga acgatcgcga aatgttaggc agcgtcggta gcgggtttat
     3841 tatgggcaat gcgatgccgc aactgcgcgc ggagctcccg catttaccgg tgattggaca
     3901 ttgccgaaat caggctgtct ctcactattt gacgcactgg ctggactatc cacatctacc
     3961 ttattccccc gaataacgag atcccttcca gcaccgggca attgcccggt ttttttgcgt
     4021 tgaatttgtc attttgtgcc gtggtgttta aaccgcacag aataaattgt cgtgatttca
     4081 cctttaaaat agaattaaaa gagaaaaaaa ttctccgtgg aagggctatg ttagataaaa
     4141 ttgaccgtaa gctgctggcc ttactgcagc aggattgcac cctctctttg caggcactgg
     4201 ctgaagccgt taatctgaca accacccctt gctggaagcg cctgaaacgg ctggaggacg
     4261 acggtatcct tatcggcaaa gtcgccctgc tggatccgga aaaaataggc ctcggcctga
     4321 ccgcctttgt gctgataaaa acgcaacatc acagcagcga atggtattgc cgctttgtca
     4381 cggtggttac cgaaatgcca gaagtgctgg ggttctggcg catggctggt gaatacgatt
     4441 atctgatgcg cgtccaggtt gccgacatga aacgctacga cgagttttat aagcgtctgg
     4501 taaacagcgt gccggggctg tcggacgtca cttccagctt cgcgatggaa cagattaaat
     4561 acaccacttc tttacccatc gaataaatat ccagaatcag gtcaggacac aacgcgtgcg
     4621 attatttgct caattaagct ggtatttccg tcgggaatgg cgtcgctatc tcggggctgt
     4681 cgccttgctt gtcattatcg cgatgctgca actggttccg ccaaaagtgg ttggtattgt
     4741 tgtcgatggc gtgacagaac aacactttac taccgggctg atcctgatgt ggatcgccac
     4801 catggtgctg attgccgttg tggtttatct cctgcgttat gtctggcgcg tattgctgtt
     4861 tggcgcgtct taccaactgg ctgttgaact gcgtgaagat tattaccgtc agctaagccg
     4921 gcagcacccg gagttttacc tgcgtcatcg cacgggtgat ctcatggctc gtgcgaccaa
     4981 tgacgtcgat tgcgtcgtgt ttgccgccgg agaaggggtg ctgacgctgg tggattcact
     5041 ggtaatgggc tgcgctgtgt tgattatgat gtctacgcaa attagctggc agttgacctt
     5101 atttgccctg ttgccgatgc cagtgatggc gatcatgatt aagcgcaacg gcgatgcttt
     5161 gcatgaacgc tttaagctgg cacaggcggc gttttccagt cttaatgacc gcacccagga
     5221 aagcctcacc agtatccgca tgatcaaagc ctttggtctg gaagatcgcc agtcggcgtt
     5281 atttgccgcg gatgccgaag ataccggcaa aaaaaacatg cgggtggcgc gtattgatgc
     5341 tcgtttcgac ccgaccatct atatcgcgat tggtatggcg aacttgctgg cgattggcgg
     5401 cggtagttgg atggtggtgc agggcagttt aacgctgggc cagctcacca gttttatgat
     5461 gtatttaggt ctgatgattt ggccaatgct ggcgctggca tggatgttta acattgtgga
     5521 acgtggtagt gctgcgtaca gccgtattcg tgcgatgctg gcggaagcgc cggtggtgaa
     5581 cgatggtagc gaacccgtgc cggaagggcg tggcgaactt gatgtaaata ttcaccagtt
     5641 cacgtatccg cagactgacc atcctgcgct ggaaaacgtc aatttcgccc tgaaacccgg
     5701 tcagatgctg ggtatctgcg ggccgactgg ttccggcaaa agtaccctgt tgtcgctcat
     5761 tcagcgtcat ttcgacgtta gcgaggggga tattcgcttt catgatattc ctctgacgaa
     5821 gttacaactc gatagctggc gtagccgcct ggcggtggtc agccagacgc cattcctttt
     5881 ttctgacacc gtggcaaata acatcgcgct gggttgcccg aatgccaccc agcaagagat
     5941 tgagcatgtc gcgcggttag ccagcgtaca tgacgatatt ttgcgtttac cgcaaggtta
     6001 cgatacagag gtgggcgagc gcggcgtgat gctttccggc gggcaaaaac agcgtatctc
     6061 tattgctcgt gcgttattag tcaacgcgga aatcctcatc cttgatgatg cgctttcggc
     6121 ggtggacgga cgcacagagc accagatcct gcataacctg cgtcagtggg gacagggaag
     6181 aacggtaatc atcagtgccc atcgcctttc ggcactgacg gaagccagtg aaattattgt
     6241 gatgcagcac ggacatatcg cccagcgtgg caatcatgat gtgctggcgc aacaaagcgg
     6301 ctggtatcgc gatatgtatc gctatcaaca actggaggcg gcgctcgacg acgctccgga
     6361 aaatcacgag gaggccgtcg atgcgtagtt ttagccaact gtggccgact ctcaagcgcc
     6421 tgttagcgta cggttcaccg tggcgtaaac cgctggggat tgcggtcctg atgatgtggg
     6481 ttgcggcggc ggcagaagtc agtgggccgc tgcttatcag ctattttatc gacaatatgg
     6541 tagcgaaaaa taacctgccg ttgaaagtgg ttgcagggct ggctgcggcg tatgttgggc
     6601 tacaactgtt tgccgccggg ctacattacg cgcagtcgct gctgtttaat cgggcggcag
     6661 taggcgtagt gcaacagttg cgtaccgacg tgatggatgc tgcgttacgc cagccattaa
     6721 gcgagtttga tacccaaccc gtcgggcagg tgatttcccg cgtcactaat gacactgaag
     6781 tgatccgcga tctctacgtt accgtagtgg caactgtcct gcgcagtgcc gcactggtgg
     6841 gcgcgatgct ggtggcgatg ttcagcctcg actggcgaat ggcgctggtg gcgataatga
     6901 ttttcccggt agtgctggtg gtaatggtga tatatcaacg ttacagcacg ccgattgtcc
     6961 gtcgtgtgcg cgcctatctg gcggatatca acgacggctt taacgaaatc atcaatggca
     7021 tgagcgtcat ccagcagttc cgtcagcagg cgcgatttgg cgaacgtatg ggtgaggcca
     7081 gtcgttcaca ctatatggcg aggatgcaaa ccctgcgcct cgacggtttt ctgctgcgtc
     7141 cgctgctgag tctgttttca tcgctcattc tttgtggatt gttgatgctg tttggcttct
     7201 ccgccagcgg caccattgaa gtgggcgtgc tgtatgcctt tatcagctat cttgggcgac
     7261 ttaacgaacc cttaatcgaa ctgaccacgc aacaggcgat gctgcaacag gctgttgttg
     7321 ctggtgagcg cgtgtttgaa ctgatggacg gaccgcgcca gcaatatggc aatgatgatt
     7381 acccgttaca gagtggcacc atcgaagtcg ataacgtgtc atttgcttat cgcgatgaca
     7441 atctggtgct aaagaacatt aatctctctg tgccttcgag taactttgtg gcgctggtcg
     7501 ggcataccgg cagtggtaaa agtaccctcg ccagtttatt gatgggctat tacccgctaa
     7561 cggaaggtga gattcgcctt gatggtcgtc cattaagttc gctaagtcac agcgcgctgc
     7621 gccagggcgt ggcaatggtg cagcaagatc cggtggtgct ggcggatacc ttcctcgcca
     7681 acgtgacgct ggggcgggat atctccgaag aacgcgtctg gcaggcgctg gaaaccgtgc
     7741 aactggcgga gctggcgcgt agcatgagcg acggtattta cacgccgctg ggcgagcagg
     7801 ggaataatct ctcagttggg caaaagcaac tgctggcact ggcgcgcgtg ctggtcgaga
     7861 cgccgcaaat cctgatcctt gatgaggcaa ccgccagcat tgactccggg actgaacagg
     7921 cgattcaaca tgctctggcg gcggtgcgtg aacataccac gctggtggtg attgctcacc
     7981 gcttatcgac cattgttgat gccgacacca ttctggtgct tcatcgtggg caagccgtgg
     8041 agcagggcac tcaccagcaa ctgctggctg cccagggacg ctactggcag atgtatcaac
     8101 tgcaacttgc gggcgaagag ctggcagcca gcgtgcgtga agaggaatca ttgagcgcct
     8161 gaatagcgca atatttcatc gttggtgcaa aaatgtaacg cactgtgcac tgtcatagtg
     8221 cgttttcatt ttcaaacttc ttaacttcct gctctctttc tcgtttttca tttctggcac
     8281 accgcttgca ataccttctt cgtgtagcag aaccattacc gaattctgac cggaggggat
     8341 ctatgaagct ggtgaccgtg ataatcaaac cattcaagct ggaagacgtt cgtgaagcgt
     8401 tatcttccat tggtattcag ggcctgaccg tcaccgaagt gaaaggtttc gggcgtcaga
     8461 aagggcatgc cgagctgtac cggggggcgg aatacagcgt caacttcctg ccaaaagtaa
     8521 aaattgatgt ggcgattgct gatgaccaac tcgatgaagt gatcgatatc gtcagtaagg
     8581 cggcttacac cggaaaaatt ggcgacggca aaatcttcgt cgctgaattg caacgcgtca
     8641 ttcgtattcg taccggcgaa gccgacgaag cggcgctgta atctctggca cacagcaaca
     8701 ggaacgaaaa atgaagatag cgacgataaa aactgggctt gcttcactgg cgatgctgcc
     8761 gggactggta atggctgcac ctgcggtggc cgataaagcc gacaatgcgt ttatgatgat
     8821 ttgtactgcg ctggtgctgt ttatgactat tccggggatt gccctgtttt acggtgggtt
     8881 gattcgcggc aaaaacgtgc tgtcgatgct gacgcaggtg acggtgacgt ttgcactggt
     8941 ctgtattctc tgggtggttt acggttactc gctggcgttt ggtgagggca acaacttctt
     9001 cggcaacatt aactggttga tgctaaaaaa catcgaactg acggcggtga tgggcagcat
     9061 ttatcagtat atccacgtgg cgtttcaggg ctcgtttgcc tgcattaccg tcggcttgat
     9121 cgttggggcg ctggcggaac gaatccgctt ctcagctgtg ttgattttcg tggtggtatg
     9181 gctgacgctc tcttacattc cgattgcgca tatggtgtgg ggcggtggtt tgctggcttc
     9241 tcacggtgcg ctggatttcg cgggtggcac ggtggtgcat attaacgccg caatcgccgg
     9301 tctggtgggc gcgtatctga taggcaaacg cgtgggcttc ggcaaagagg cgtttaaacc
     9361 gcacaacctg ccaatggtct tcaccggtac tgccattctc tatattggtt ggtttggctt
     9421 taacgccggg tcagcgggca cggcgaatga aatcgcggca ctggcatttg tgaatactgt
     9481 ggtcgcaacg gcggcggcaa ttcttggctg gatcttcggt gaatgggcgc tgcgtggtaa
     9541 gccttctctg ctgggagcgt gttctggcgc gattgccggt ctggtcggcg tgacgccagc
     9601 gtgtggctac attggggttg gcggcgcgtt gattatcggc gtggtagctg gtctggcggg
     9661 cttgtggggc gttactatgc tcaaacgctt gctgcgggtg gatgatccct gcgatgtctt
     9721 cggtgtgcac ggcgtttgtg gcattgtcgg ctgtatcatg actgggattt ttgccgccag
     9781 ttcgctgggt ggcgtgggct tcgctgaagg tgtgacgatg ggccatcagt tgctggtaca
     9841 gctggaaagc atcgccatta cgatcgtctg gtccggtgtg gtggcattta ttggctacaa
     9901 actggcggat ctgacggttg gtctgcgtgt gccggaagaa caggagcgag aagggctgga
     9961 cgtcaacagc cacggcgaga atgcctataa cgcgtaacaa gcactgcaaa aaacagccgg
    10021 acggttttca cctccggcta tttttttaat tgtgattacg catcacccct tcctgaacgg
    10081 tcgaggcaac cagcacgccg tcctgagtat aaaactcacc gcgcacaaag ccgcgtgcgc
    10141 tggacgccga ggtgctctcc acgctataca gcagccattc attcaaatta aacgggcgat
    10201 gaaaccacat ggaatggtca atggtggcaa tctgaatccc cggttcgaga aaaccgatgc
    10261 cgtgcggctg tagagctacc ggcaggaagt taagatcaga agcgtaaccg agcagatact
    10321 gatgaacgcg caggtcatcc ggcacgctac catttgcgcg gatccacacc tgacgatgtg
    10381 gttctgcgac gtgacctttc agtgggttat gaaactccac cggacggact tccagcggac
    10441 gatcgcagat gaatttatct ttcagcactg gcggcagcag gtgcgccagc gattgggcga
    10501 tttgtgtttc cgaaggcagg ccatcaggcg ctggcgcgga cggcattgtt ttttgatgtt
    10561 cgaaacccgc ttcaggtgcc tggaaagagg ccgtcatata aaaaatcggt ttgccgtttt
    10621 gaatagcagc aacccggcgg gcgctgaagc tgttaccgtc acgcagcgtt tcgacatcat
    10681 aaataatcgg cttcttacta tcgccagggc gaagaaagta gctgtgaaac gaatgtacca
    10741 gccgctcttc agggacggtc tcttttgcag catacaaggc ctgacccacg acctggccgc
    10801 caaacacctg gcgtaaacct aaatcttcac tctggccgcg aaagagtcct tcctcaattt
    10861 tttccagatt taacaatgtc agtaaatttt ttagcgcctg actcatataa ctctccagta
    10921 actaagctgc cgcagcaagc caaagtgagt tgagtataac gcaaatttgc cactggtccg
    10981 atgggtgcaa tagtctgaat tacgggctaa ttacaggcag aaatgcgtga tgtgtgccac
    11041 acttgttgac gttactattt tgttaaccac tcttccggcg aggaaagtta gcccgctggt
    11101 gcattgataa taaggagaag tgaatgaaac tcgtgcacat ggccagttgt ttagcggttg
    11161 cgattgcgtt ggcggcttgc gcagataaaa gcgcggatat tcagacgcca gcaccggcgg
    11221 caaatacgtc tatttcagca acacaacaac ctgctatcca gcaaccgaat gtctccggta
    11281 ccgtttggat ccgtcagaaa gtcgcactgc cgcctgatgc tgtgctgacc gtgacacttt
    11341 ctgacgcgtc gttagccgat gcgccgtcaa aagtgctggc gcagaaagcg gtgcgtaccg
    11401 aaggtaaaca gtcaccattc agctttgttc tgccttttaa cccggcagat gttcagccga
    11461 acgcgcgtat tctgttgagt gcggcgatta ccgtgaatga caaactggta tttattaccg
    11521 ataccgttca gccggtgatc aacaagggcg gaactaaagc cgacctgaca ttggtgccga
    11581 tgcagcaaac cgccgtgccg gttcaggcca gcggtggtgc aacgactacc gtaccttcga
    11641 cttcaccaac tcaggtgaat ccgtcttcgg cagttccagc tcctacgcaa tattaagcga
    11701 cgttaaccct ctccgcccgg agagggttag tagttccagc gataacgctg caagtcgatt
    11761 tgcccgcttc ccgataccat cacaccttct gccagtaatg cctgtcgctg acgctgtaaa
    11821 tccggtccgg ttagcgaaat tgtgccgtgg cgattaacca cccggtgcca gggtaaggtg
    11881 ctgccttcag ggagacgctt taacacaccg cccacctggc gcgcggcgcg gggcgatccc
    11941 gccagtttcg ccacatcacc gtaagtggtg acatagcctt cgggaatagc ggcgacgatt
    12001 tgccagacgc gttggggaaa tgaatcttct ttttccatct tttcttcctg aggtaatttt
    12061 tcagcataat ctggaaaaac gcccgagtga agtcgcattg cgcaagaaac cagcatctgg
    12121 cacgcgatgg gttgcaatta gccggggcag cagtgataat gcgcctgcgc gttggttctc
    12181 aacgctctca atgggggctc tgttggttct cccgcaacgc tactctgttt accaggtcag
    12241 gtccggaagg aagcagccaa ggcagatgac gcgtgtgccg ggatgtagct ggcagggccc
    12301 ccacccattt ctgcctccca ccgtttcgtc aaaaaatccc aacatagcta aactttaacc
    12361 acaactgacg tcgcaagaat tgtctggctg cgcagtacgc ttcggaggta tgtctgatga
    12421 agtatgttga tggttttgtg gttgccgttc ctgccgataa aaaggatgcc tatcgggaaa
    12481 tggccgctaa ggctgcgcca ttgtttaaag agtttggcgc gcttcgtatt gtcgaatgct
    12541 gggccagcga tgtaccggat ggcaaagtga ccgattttcg tatggcggtg aaagcggaag
    12601 agaatgaaga ggttgtcttt agctggattg aatacccttc aaaagaggtc cgcgacgctg
    12661 ctaatcaaaa gatgatgtcg gacccacgga tgaaagagtt cggcgagtcc atgccgtttg
    12721 acggcaagcg aatgatctat ggcggattcg agtcaatcat cgacgaatag gtagcgtgac
    12781 gggctgtgcg gcgtgccgtc agcccaaaaa ttcacaaatg ttgctcggcc cagcgtaaga
    12841 aatcttcttt cggtaatgcc ttgctgtaga gccagccctg accgtagtgc acgccatgct
    12901 ggcgtagcca ctcttcttgt ttactggttt cgattccctc cgctaccatt ttcagtttca
    12961 gtgtttttgc catttcgatg atatgcggcg tgacattttt atattccagc gcatcaacga
    13021 aagatttatc gatcttcaga atgtcgacat ccagatcctg taaataactt aaacttgaat
    13081 atcccgtacc aaaatcatca agataaattt catggcccgc ctcccggtag cgagaaatta
    13141 tcggggcgct ggttttcgga tcggcaaact cgcgttcagt gagttcaagc gcgatctgtc
    13201 tgggattaac ctgattgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg
    13261 agcacggtgg attcaagatt gatcgaaata tgctgctgtg gatgctgacg cagccagtcg
    13321 cccatatctt caaaggcgct tcttataatc agtagcgtca atggctcaga aaggcccgtt
    13381 tgctgtgcca gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag
    13441 cgcgccagtg cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag
    13501 tgcacgcaaa tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata
    13561 cggcgcaaaa tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgagcggt
    13621 aaccaaataa agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg
    13681 atgatcgaaa tattcatctc cgggaagggc aggatatcgt aaatgattcc attattttcg
    13741 atatgctcac cgggtgtttt ttgtagcctg gtaataattc cctgagcaat ttcatcgctg
    13801 ctggttatga caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta
    13861 tagggaatga catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc
    13921 atggcgacca tgtaacggat aatgcctaaa tcgttatgcg atgttaacca gacacgataa
    13981 ccatctttcg aaattttacc tggctcgggg aaggtatcgg gcggactttc atgctccaga
    14041 gacgaacact ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg
    14101 taagataccc gacgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt
    14161 tccagctctt gcagcgcatc tttcccttgt gtcgccacct tattggcccg aatagcgacg
    14221 cgggaggaat aggtatccag ctcttcaata aacgatgttt ctacctgctg atgggccagc
    14281 cagatgctta agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg
    14341 accagatgtc gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct
    14401 gattgtggaa aaaggctggc aggagataag gaggtagagc tggtatttca tgaagtgatg
    14461 ggtggaaacg atcattgtca tttgaccaag cgcagagctt tacgcctggt atgtaaccct
    14521 aacttaagat gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag acagttcacc
    14581 tttacggacg atcatttttt gcagttgttc cttatgaaca tccgcgacac gcggagagtt
    14641 caagatggct tcaaccagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata
    14701 gcaaacgcgg aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa
    14761 aaagaagaaa ccattaagca ggataaataa tacaaccagt agccagacag agcctgacag
    14821 cgtttcggac tgcaacatca ccgccagggt ggcaaaaaaa gcaacgtaca taccgataaa
    14881 cagccccgga tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc
    14941 gcgggtattc agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg
    15001 ccttcgtggt atctgcaact ttccagggaa caccctattt taacgtggga atagtagagg
    15061 ggaagtaaca gatttaccac aattttgcat aacagttgcg aaaaaccgat tacaattttt
    15121 taattattct ggctggatta ccgcccacga caacgttgtc cgggacatct tttgtgacaa
    15181 ctgcacctga ggctaccacg acgttatcac caatggtcac accagggtta atgaccgcgc
    15241 gtccgccaat ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat
    15301 tacgtgctac agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca
    15361 tacagttatc accgatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga
    15421 aaaaattatt accgagaaaa atgttatagc catagtcaca gcgaaacgtt ggctcaatat
    15481 aagcctctgt cacctgaccg aatagatcag cgagaatttg ctggcgtaat gtgtgctctt
    15541 ccgccaggga atgattgtat cggtgaataa gctgacgagc gcgcaggcga tcgcgagata
    15601 acgtctcatc tgccgagcga tacaactcac cagcaatcat cttttctttt tctgtgctca
    15661 ttactgacct ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag
    15721 aacgttccca ttacatctgt gtgataaaga tcacataggg tatgtcttca tggtgaaaag
    15781 tataaaattc ttaataaaca gccggttata gctccgaaag cgaattattt attagcgaat
    15841 aaatttccat actgaggaag ggatcttgtc gtatagttta ttcatggtca attcggcgag
    15901 gcggtgatct gcggctgagt aaaataccgc cagttcatta tctgataatt cgtatttatt
    15961 tttctcgata acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata
    16021 atcggttttc gttaaaggtt tttcggacat acttctaccc atggttgtaa taattctaac
    16081 aagataaact cgcaggattc tctttcgtcg cattgacaaa acaacggaat agtcgattac
    16141 ctgacttccg ccatttctga agatcctgca tattaatacc ataactactg aacaacataa
    16201 aggtgtcatc cagatattcg tcgatctgct caatgagctt attgtcttca ttatacttaa
    16261 ttttgtaatt aagtgcgaag gtctcaatat gctcaatcag ttcattcaac tggaggttga
    16321 tcgccgaggt tgggtcgtta acccagccat gattgctttc ttcaaggttt gcaaggcagt
    16381 catgatacag ggtttcacag agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt
    16441 attcatccat aacgcgtccc cttcttagcg gttgaactaa cggacacctt tcgggatgga
    16501 aaaaacttac tgacctggac ttgccctctc gtttgttagc ttaactataa gccactcttt
    16561 gcaggttttc atcgcattat tacgaaaaat tacaattagg aaggaatcgg gacaggactc
    16621 actttttcat actagcacta acccgcagca ggtaaaagca gtctgctcga actgttcata
    16681 acatttcagg attatcgcca gatgaaattg gtgacactaa taccaggatt gctctgaata
    16741 tgacgtaata accgaggaat gaataaagaa ttaccgcaaa taattaagaa tagcctctaa
    16801 atgattatgg attattaaat ctattagcgc tacttaatat aaattaacta acaatcagta
    16861 ttttttatga attttatccg tggttaatac tggttttcgt atgagatcct gagttggtgg
    16921 ttcaattact ccttaatgtt cgtaggttat gcataaaaaa ggccgcttgc gcggccttag
    16981 tgattacacg ttgtatcaat gatgatcgac agtatggctg tgctcgatat cttcattctt
    17041 gcggctaaag cggcggcgaa ccaccacaaa gaataccgga acgaagaaga ttgccagtac
    17101 cgttgcggtc accatcccgc ccattacacc ggtacctact gcgttctgcg cgccggaacc
    17161 agcaccagta ctgataacca gcggcataac gccgaggata aacgccagcg aggtcatcag
    17221 gatcggacgt aaacgcatcc gcactgcatc aagcgtcgct tcaatcagac ctttaccttc
    17281 tttatccatc aagtctttgg cgaattcgac gataagtatc gcgttcttcg ccgacaaccc
    17341 aatggttgtg agcaggccta cctggaagta aacgtcattg gtcaggccac ggaaggtggc
    17401 agccagcaac gcaccgataa cccccagcgg aacgaccagc ataacggaga acggaatcga
    17461 ccagctctcg tacagcgccg ccagacacag gaatacgaca atcaacgaaa tcgcgtacag
    17521 tgaaggtgcc tggttgccgg agagacgttc ctgataggac atccccgtcc agtcatagcc
    17581 aacaccggta ggcagtttgc tcgccagttg ttccatcagc tccattgctt caccggtact
    17641 tttacccggt gccgcctggc ctaagatttc catggatggc aggccgttgt aacgttccag
    17701 acgcggcgaa ccgtactccc aacgagaaga ggagaacgcc gagaatggca ccatctgacc
    17761 atcagcagca cgaacatacc agtcgccgat atcatccggc agcatacggt atttcgcttc
    17821 tgacatgacg taaactttct tcacacgacc gcggtcgata aagtcgttca catagctacc
    17881 gccccatgca gcgcccagag aggtgttaat gtcgttgata gaaacaccca gcgcctgcgc
    17941 tttttcctgg tcgatatcaa tcttaaactg cggggtatct tccagaccgt ttggacgtac
    18001 gctggtcagc atatcagggt gcttcgctgc ttctgcaagc agctggttac gcgcctgagt
    18061 cagtttttcg tgaccaaggc cagcctggtc aatcagctca aagtcaaagc cggttgcagt
    18121 acccagttcc acgattgcgg gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga
    18181 gaaagcgcgt gttgcacgca tggtaatcgc ttcaactttg ttttcttcgc ccggacgatc
    18241 ggcccagtcc ttcaaggaaa cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc
    18301 gaagccgtta acggcgaaca ccgactcaac gttgttcttt tctttggtca gatagtaatg
    18361 cgttacctca ttgagcactt tctgcgtacg ttcctgcgtt gcacctgctg gcagctgaac
    18421 catggtcata aatacgccct ggtcctcatc tggcaagaag gagcttggca gacgcacgaa
    18481 cagataggcc atgccgacca cgatgatcag atacagcacc aggtaacgcc ccgtactgcg
    18541 cagaataccg cctacgctgt cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttaaa
    18601 ccagccgaag aagccttttt taccttcccc gtgatcgcct ttggcaatcg gtttcagcat
    18661 ggtggcacaa agagccggag tcaggatcaa cgctaccagt accgacagcg ccattgctga
    18721 aacaatggta atagagaact gacgatagat agcaccagta gaaccgccaa agaaggccat
    18781 cggtacgaat accgccgaca gtaccatcgc gataccgacc agagcgccct gaatctgccc
    18841 catcgactta cgggtagctt cttttggcgg caaaccttct tccgccataa cacgctccac
    18901 gttttctacc acaacgatag cgtcatccac caacaagccg atggcgagca ccatcccgaa
    18961 cattgttagc gtgtttatcg agaagccaaa ggcggcaagg acggcaaagg tcccgagcaa
    19021 taccaccggt acggcaatgg tcggaatcaa cgtcgcgcgg aagttctgca ggaacagata
    19081 cataaccagg aacacgagga tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga
    19141 gattttcacg aacggcgtgg tgtcgtacgg gtaaacaatt ttcagacccg acgggaagaa
    19201 cggttccatc ttcgccagtt cagcacggat tgccgcagcg gtatccagcg cgtttgcacc
    19261 ggtcgccagc ttgatcccca gaccggaagc cggttggccg ttaaactctg cgatgatgtc
    19321 gtagttctca ccacccagct caatcttcgc cacatcacgc agcagtacgc gggaaccatc
    19381 ctgattcact ttcagcagga ttttgccgaa ctcttcagta gaggtcagac gcgtctgagc
    19441 aataatagag gcgttaagct gttggccttt caccggcggc gtaccaccga gctgacccgc
    19501 cgcaacctgg gcgttctgcg ctttgatggc ggtaatgaca tcaaccggcg ttagctggaa
    19561 tttgttcagc tcattcgggt tcatccagat acgcatcgcg tactgtgaac cgaacaactg
    19621 aacatcaccc acgcccgacg tacggctgat ggcatctttc atattcgccg ctacgtagtc
    19681 agagatatcc tcctgcgtca tggtgccatc ggtgttgata acgccgacaa ccatcaggaa
    19741 gctgctggat gatttctcaa cgctcacccc ttgctgctga acttcttgcg gcagcaacgg
    19801 catcgccagc tgcagtttgt tctgcacctg aacctgcgcg atatccgcat cagtaccaga
    19861 ctcaaaggtc agggtgatct gcacggtacc cgtggagtca ctgttagagg acatgtacat
    19921 caggttatcg ataccgttca tattctgttc gataacctgt gtcaccgtgt cctgcactgt
    19981 tttcgcatca gcgccggggt aggaggcgga gatcgttact gccggcggtg caatcgtagg
    20041 atattgcgcc accggcagtt tgaggatcgc cagcccccct gccaacatga tgataatggc
    20101 gatcacccac gcaaaaatcg ggcgatcgat aaagaaatta ggcatgtctt aacggctcct
    20161 gtttaagtta agacttggac tgttcaggct gagcaccgct tgcggcttgc tggttattat
    20221 cagcggtaac ttcttgtgct tttacctgga caccaggacg cactttctgc agcccactta
    20281 ttactacgcg atcgcctgct ttcagacctt ctgtcaccag ccacttatcg ccaatagcct
    20341 ggcttgcaac gatcggacgg gtttccactt tgtcatccgc gccaactacc agtacggtgg
    20401 catcaccacg cggcgtacgg gttacgccct gttgcgggac taaaatagcg tttggattaa
    20461 gcccttcttc cagacgtgcg cgcacgaaca tacccggcag cagagtgtga tccgggttcg
    20521 ggaagatagc gcgtagggtg atagacccag tggtctgatc aacggtaacg tcagagaatt
    20581 ccagcgtacc gtcctgcggg aacttaatgc cgtcactggt gatcagcgac actttggctt
    20641 tgccgttctc ttgtttcagc gtgccattcg ccagttcctg tttcaggcgc aggaagtcgt
    20701 tgctggactg ggtcacatca aggtagatcg gatcaagttg ctgcacggtt gccagcgcag
    20761 tcgcctgacc gttctgtacc aatgcgcctt ccgtcacgtt cgacttacca atgcgaccgc
    20821 taatcggaga ggtgactttg gtgtaagcca gattgatccg cgcagtttca acggcagctt
    20881 tcgccgcagt taccgcagca ttcgcctgtt gcgcatcagc cagagcctga tcgtactctt
    20941 gcttactgat gtactgagta ccgagcagtt tctgataacg attcaccgtc aattgcgcga
    21001 tattggctgc agcctgggct ttcgccagat cacctttcgc actgtcgtat gtcgcctgat
    21061 aggtcgcagg atcaatctga tagagagaga caccagcttc gatgtcgcta ccttctttga
    21121 aattacgctt caggataatc ccgctaactt gaggacgaac ttctgcgatc cggtaggcac
    21181 tggtgcgacc cggaagctcg gttgtgatct gcagaggttc agttttgact gttactacgc
    21241 caacggcggg catctgctgg ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg
    21301 ctaagctgcc tgagagcatc agaacgaccg ccagaggcgt aaaccctctg tttttgttca
    21361 tatgtaaacc tcgagtgtcc gatttcaaat tggtcaatgg tcaaaagtta ataaacccat
    21421 tgctgcgttt atattatcgt cgtgctatgg tacatacatt cacaaatgta tgtaaatcta
    21481 acgcctgtaa attcacgaac atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg
    21541 ccaacacatc ctcgatgtgg ctctacgtct tttctcacag cagggggtat catccacctc
    21601 gctgggcgag attgcaaaag cagctggcgt tacgcgcggt gcaatctact ggcattttaa
    21661 agacaagtcg gatttgttca gtgagatctg ggaactgtca gaatccaata ttggtgaact
    21721 agagcttgag tatcaggcaa aattccctgg cgatccactc tcagtattaa gagagatatt
    21781 aattcatgtt cttgaatcca cggtgacaga agaacgacgt cgattattga tggagattat
    21841 attccacaaa tgcgaatttg tcggagaaat ggctgttgtg caacaggcac aacgtaatct
    21901 ctgtctggaa agttatgacc gtatagaaca aacgttaaaa cattgtattg aagcgaaaat
    21961 gttgcctgcg gatttaatga cgcgtcgggc agcaattatt atgcgcggct atatttccgg
    22021 cctgatggaa aactggctct ttgccccgca atcttttgat cttaaaaaag aagcccgcga
    22081 ttacgtcgcc atcttactgg agatgtatct cctgtgcccc acgcttcgta atcctgccac
    22141 taacgaataa ccctgaatct gactccagga tttttcctgg acattttcgt cgttgctatt
    22201 ctggttcact gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt cttcaggttc
    22261 agaaaccttc attcatcatg actatgttcc agtattacaa acgatcacgg cattttgttt
    22321 tttcagcatt tattgctttt gtttttgtct tgttatgcca gaacacggcg tttgcgcggg
    22381 cgtcatcgaa tggtgatctg ccgacaaaag cggacctgca ggcgcaactt gactcactaa
    22441 ataaacaaaa agatctttct gctcaggaca aactggtgca gcaggatctg acagatacat
    22501 tagccaccct cgataaaatc gatcgcataa aagaagagac agttcagcta cggcaaaaag
    22561 tcgctgaagc gccagaaaaa atgcgccagg cgaccgcggc gttaacagca cttagcgatg
    22621 tcgataacga cgaagaaacg cgcaaaattc tgagtacgct ttcgttgcgc cagctggaaa
    22681 ctcgcgttgc ccaggcgctg gacgatttgc aaaacgcaca aaacgatctg gcgtcttata
    22741 acagccagct ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg atgtataacg
    22801 cttcgcagca gctgcaacaa attcgcagtc gtctggatgg gactgatgtc ggcgagactg
    22861 ccttacgtcc cagccagaaa gtgttaatgc aggctcagca ggcgttgctg aatgcggaga
    22921 ttgaccagca gcgtaaaagc ctggaaggga acaccgtctt gcaggatacc ttgcaaaagc
    22981 aacgtgatta cgtgacggcg aacagcgccc gtctggagca ccagttgcag ctgttgcaag
    23041 aagcggtaaa cagcaagcgc ctgactttaa ctgaaaaaac ggcgcaggaa gccgtctccc
    23101 cggatgaagc cgcgcgtatt caggctaatc cgctggtgaa acaggaactg gaaattaacc
    23161 agcagttaag tcagcgtctg attaccgcga ctgaaaacgg taatcagttg atgcagcaaa
    23221 acattaaagt caaaaactgg ctggagcggg cgctgcaatc ggaacgcaat attaaagagc
    23281 agattgccgt cctgaagggc agcctgctgt tgtctcgtat cctttaccag caacaacaaa
    23341 cgctgccctc ggcggatgaa ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg
    23401 aacagtttga agttaaccag cagcgtgatg cactcttcca gagcgatgcg ttcgtcaaca
    23461 aactggaaga aggtcacacc aacgaagtca acagcgaagt tcacgatgcg ttattgcaag
    23521 tggttgatat gcgtcgcgaa ttgctggatc aactcaacaa acagttgggt aaccagctga
    23581 tgatggccat taacctgcaa atcaaccagc agcagttaat gagcgtatcg aaaaacctga
    23641 aatccatcct gactcagcaa atcttttggg tgaacagtaa ccgtccaatg gactgggact
    23701 ggatcaaagc gttcccgcaa agcctgaaag atgaatttaa gtcgatgaaa atcacggtga
    23761 actgggaaaa agcctggcct gccgttttta tcgctttcct cgccggtttg ccgctgctgt
    23821 tgattgccgg gctgatccac tggcgtctgg gctggctgaa agcgtatcaa cagaaactgg
    23881 cttccgctgt gggttccctg cgtaacgata gccagctcaa cacaccaaaa gcgatcctta
    23941 tcgacctgat ccgcgcgctg ccggtgtgcc tgattattct cgcggttggc ctgattctgt
    24001 tgaccatgca gctcaacatc agcgaactgc tatggtcgtt cagtaaaaaa ctggcgattt
    24061 tctggctggt gtttggcctg tgctggaagg tactggagaa aaatggcgtt gccgtgcgtc
    24121 acttcggcat gccggagcag cagaccagcc actggcgtcg gcaaattgtc cgcatcagtc
    24181 tcgcgttgct gcctatccat ttctggtctg tggtggcaga actttccccg ctgcatctga
    24241 tggatgatgt gctggggcag gcgatgattt tcttcaacct gctgctgatt gccttcctgg
    24301 tatggccaat gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg cgactggtca
    24361 ccattaccgt gctgtcgatt atcccgattg cattgatggt gctgactgcc acgggttatt
    24421 tctacaccac gctgcgcctc tccgggcgtt ggattgaaac cgtttatctg gtgatcatct
    24481 ggaacttgct ctaccagacg gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct
    24541 ggcgtcgtgc gctggcgcgt cggcagaatc tggtgaaaga gggcgcagaa ggtgccgaac
    24601 cgccggaaga acccaccatt gcactggaac aggttaacca gcagacgctg cgtattacca
    24661 tgttgctgat gtttgcgctg ttcggtgtca tgttctgggc aatttggtcc gatttgatca
    24721 ccgtgttcag ctatctcgac agcatcacgc tctggcatta caacggcact gaagctggcg
    24781 ctgcggtggt gaaaaacgtc accatgggca gtctgttgtt tgcgattatc gcctcaatgg
    24841 tggcctgggc gttgattcgc aacctgcctg gtttactgga agtgctggtg ctctcgcgac
    24901 tgaatatgcg tcagggcgcg tcgtatgcca ttactaccat ccttaactac atcattattg
    24961 ccgttggtgc gatgacggtg ttcggatcgc tgggcgtctc ttgggataaa ctccagtggc
    25021 tggccgcagc attatccgta ggtcttggtt ttggtttaca agaaattttc ggtaacttcg
    25081 tctccggttt gatcattctg ttcgaacgtc cggtgcgtat tggcgatacg gtaaccattg
    25141 gtagcttctc ggggacggta agtaagatcc gtattcgtgc gacaacgatt accgatttcg
    25201 atcgcaaaga agtgatcatc ccgaacaaag cgtttgttac cgagcgtctg atcaactggt
    25261 cgttgactga cactactacg cgtctggtga tccgtctcgg cgtggcctat ggctccgatc
    25321 tggaaaaagt gcgtaaagtg ttactgaagg cggcgactga gcacccaagg gtgatgcacg
    25381 aaccaatgcc ggaagtcttc tttacggcgt ttggtgccag cacgttggat catgagctgc
    25441 gtctgtatgt gcgtgaactg cgtgaccgta gtcgtactgt cgatgagctg aaccgtacta
    25501 tcgatcagct gtgccgtgaa aacgacatca acattgcctt taaccagctt gaagtgcatc
    25561 tgcacaacga gaagggcgat gaggtgacgg aagtcaaacg cgactacaaa ggcgatgacc
    25621 cgacgccagc ggtagggtaa taaccaaagg gggcgacatt tagttgcccc gagattactg
    25681 acaaagtgcg cgttgttcat gccggatgcg gcgtgaccgc cttatccggc ctacgaaacc
    25741 gcaagaattc aatgtattgc aggagcggtg taggcctgat aagcgtagcg catcaggcag
    25801 ttttgcgttt gcccacaatc ttaggggaca tttagcggcc ccatttattt ctcacttttc
    25861 cgccttatca tcgcgcgtta atttcttttc ataatcgcgt tttacaatat ccagcgcgcg
    25921 cagcacggtg cgggctggga tctgattttc ttccagcagc acaatcaaat cgacagccag
    25981 tttgacatcg tcaggggcat tttcaagtga catactctct ccattgctag cgggttaagc
    26041 gcgctagcct gttttcgatt ttttccagcg catggcggca gcgtgccagg cgcgcttcat
    26101 aggcttccac ttcacgatgc agcgtttgct gttccacgag atcggtcacc cgcgccaggc
    26161 gggctctgcg ttcggcaacc atctcacgta gccgccgctc aaaatcctga tgctgaatac
    26221 gtttacgctg ccagcgggca attttcggtg gcgcactgtc ccactcgcgc aatgaccagg
    26281 cggaggcttc acgcgcgatg gcttccagtt gtgccgccag atgttccgcc agccaggcca
    26341 cttgcggcag ctgttgctgc tcaaccgcat gacaaagcgc agccagatta tcgcccgcct
    26401 cgtcgagaca agcctgtagt gttgtcgcac gagtctgaaa aagatgcctg tcgaaacgag
    26461 cacttagcgt ggcgaactgt gacaccgggg cacaacgctg acgcagcgta gcgagctgtc
    26521 cttccagttt ttccagcagc agggcggttt tcacgataag acctcaatga aaatgataat
    26581 tgttatgcta aagtagccac tctgtaagct gacaacactc aatatgcaac gaatcatttt
    26641 aatcatcatt ggctggctgg cggtagtgct gggtacgctg ggcgtggtat taccggtatt
    26701 accgacgacg ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt
    26761 tcacgcctgg ttgctgtacc gctcatggtt tggcagctat ctacgtttct ggcagaaaca
    26821 tcatgcgatg ccgcgcggcg tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc
    26881 catttctctg tggttcgtcc agatgccatg ggtgcgcatc atgttgctgg taattctcgc
    26941 ctgtttgctt ttctatatgt ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg
    27001 aagcacaaca atcgcagttg caattattgc gtacagccag tacattctgg cgttttcgag
    27061 cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc
    27121 gtgagtacca ccgtaacaag caggcataca cttatgaccg cgactgcaca gcagcttgag
    27181 tatctcaaaa atagcatcaa aagcattcag gactacccaa aacccggcat tcttttccgc
    27241 gatgtcacca gcttactgga agacccgaaa gcttacgctc tcagcatcga cttgctggtt
    27301 gagcgttaca aaaatgcggg cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg
    27361 tttggcgctc cggtagctct gggtctgggc gttggctttg taccggtccg taaaccgggc
    27421 aaactgccgc gtgaaaccat cagtgaaact tacgacctgg aatacggcac cgatcagctg
    27481 gagatccacg ttgatgccat caaaccgggc gacaaagttc tggtggtgga cgatctgctg
    27541 gcaaccggcg gcactatcga agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg
    27601 gctgacgctg cgttcattat caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa
    27661 cagggcatta ccagctacag ccttgtcccg ttcccgggcc attaattatc gccagtcttg
    27721 tgctgcccac gctacggaca gcacaagatg tgcattcagc ctcgccgttc tgacggggct
    27781 gtgttagcat taccccttcg tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt
    27841 atcaggtctt agcccgaaaa tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac
    27901 atgtgctgac cgcactggcg aacggcttgt cgttagggcg tattcatcat gcttatcttt
    27961 tttccggcac ccgtggcgtc ggaaaaacct ctatcgcccg actgctggcg aaggggctaa
    28021 actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg
    28081 agcaggggcg ctttgtcgat ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag
    28141 atacccgcga cctgctggat aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt
    28201 atctgatcga cgaagtgcat atgctgtcgc gccacagctt taacgcactg ttaaaaaccc
    28261 ttgaagagcc gccggagcac gttaagtttc tgctggcgac gaccgatcca cagaaattgc
    28321 cggtgacgat tttgtcgcgc tgtttgcaat ttcatctcaa ggcgctggat gtcgagcaaa
    28381 ttcgccatca acttgagcac atcctcaacg aagaacatat cgctcacgag ccgcgggcgt
    28441 tgcaattgct ggcgcgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc
    28501 aggcgattgc cagcggtgac ggccaggttt caacccaggc agtcagtgcg atgctgggta
    28561 cgcttgatga cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc
    28621 gcgtaatggc gctgattaat gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg
    28681 tggaaatgct cggcctgttg catcgtattg cgatggtaca actttcgcct gctgcacttg
    28741 gcaacgacat ggccgccatt gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga
    28801 cggatattca gctttactat cagacgctgt tgattggtcg caaagaatta ccgtatgcgc
    28861 cggaccgccg catgggcgtt gagatgacgc tgctgcgcgc gctggcgttc catccgcgta
    28921 tgccgctgcc tgagccagaa gtgccacgcc agtcctttgc acccgtcgtg ccaacggcag
    28981 taatgacgcc aacccaggtg ccgccgcaat cagcgccgca gcaggcaccg actgtaccgc
    29041 tcccggaaac caccagccag gtgctggcgg cgcgccagca gttgcagtgc gtgcagggag
    29101 caaccaaagc aaaaaagagt gaatcggcag ccgctacccg cgcgcggccg gtgaataacg
    29161 ctgcgctgga aagactggct tcggtcaccg atcgcgttca ggcgcgtccg gtgccatcgg
    29221 cgctggaaaa agcgtcagcc aaaaaagaag cgtatcgctg gaaggcgacc actccggtga
    29281 tgcagcaaaa agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa
    29341 aaacgccgga actggcggcg aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg
    29401 cacaggtgag ccaactttcg ctaccaaaac tggtcgaaca ggtggcgtta aatgcctgga
    29461 aagaggagag cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca
    29521 accgcggtgc acagcaaaaa ctggctgaag cgttgagcat gttaaaaggt tcaacggttg
    29581 aactgactat cgttgaagat gataatcccg cggtgcgtac gccgctggag tggcgtcagg
    29641 cgatatacga agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc
    29701 agaccctgcg tcggtttttc gatgcggagc tggatgaaga aagtatccgc cccatttgat
    29761 cgtaagcaca gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt
    29821 aaaggcggtc tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa
    29881 atgcaggaag agatcgcgca gctggaagtc accggcgaat ctggcgcagg tctggtaaaa
    29941 gtgaccatca acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa
    30001 gacgacaaag agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt
    30061 attgaagaaa cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct
    30121 ggctttaaga tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac
    30181 tgcgctgtct gccgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc
    30241 agcgcgatcg tagcggcggg atgcgtttgg cgcaggcgct cacccgggcg atgtcggaaa
    30301 tcggccactg cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga
    30361 atccgcgtcg tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatctacg
    30421 ccattgagca gacggggcag ttttctggtc gttattttgt gttgatgggg catctgtcac
    30481 cgctggacgg catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag
    30541 aggaaaaaat cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg
    30601 ctaactacat tgccgagctt tgcgcgcaat atgacgtgga agccagccga atcgctcatg
    30661 gcgtaccggt tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactcccttg
    30721 ccgggcgtca taagattcgt ttttaagcaa acgagagcag gatcacctgc tctcgcttga
    30781 aattattctc ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta
    30841 ttgaggtaga cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag
    30901 cttctgcacc tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt
    30961 atctctaacg cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac
    31021 ctgtacgaag gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg
    31081 ctgaccatct ctgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg
    31141 actatcgcta aatccggtac caaatcattc ctcgaatccc tgggttctga ccaggcgaaa
    31201 gacagccagc tgatcggtca gtttggtgtt ggtttctact ctgcgtttat cgtggccgac
    31261 aaagtgaccg tgcgtactcg cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa
    31321 tcggctggcg aaggtgaata taccgttgcc gacatcacca aagaagatcg tggtactgaa
    31381 atcaccctgc atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc
    31441 atcatcagca aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag
    31501 aaagacggcg aaaccgttat ctcctgggag aaaatcaaca aagcgcaggc gctgtggact
    31561 cgtaacaagt cggaaatcac cgatgaagag tacaaagagt tctacaaaca catcgcccac
    31621 gactttaatg atccgctgac ctggagccac aaccgcgttg aaggtaagca ggagtacacc
    31681 agcctgctgt acattccgtc ccaggctccg tgggatatgt ggaaccgtga tcataaacac
    31741 ggcctgaaac tgtacgtcca gcgtgtgttc atcatggacg acgcagaaca gttcatgccg
    31801 aactatctgc gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc
    31861 cgtgaaatcc tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt
    31921 gtgctgcaaa tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg
    31981 caacagtttg gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg
    32041 atcgccaaac tgctgcgttt tgcttctacc cataccgatt cttctgcgca gaccgtatct
    32101 ctggaagact acgtttcccg catgaaagaa gggcaggaga aaatctacta catcaccgca
    32161 gacagctatg cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc
    32221 gaagttctgc tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc
    32281 gacggtaaac cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac
    32341 gaagttgatg agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgtgtg
    32401 aaagccctgc tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg
    32461 ccagcgatcg tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct
    32521 gcggcgggcc agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta
    32581 ctggtgaaac gtgcggcaga tactgaagat gaagcgaagt tcagcgagtg ggtagaactg
    32641 ctgctggatc aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt
    32701 cgtcgtatga accagctgct ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg
    32761 gcatttttct tttcatcatc tgcactttcc gcaaattatc tcgccattaa ccgtttcagc
    32821 ctcaggtgcc tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa
    32881 aaatcgacac attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc
    32941 gcggggaaag ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc
    33001 actggcgata tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa
    33061 gacattatgg atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag
    33121 cgcattgctc aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt
    33181 ccgcaggcag acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac
    33241 gtaccggacg aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt
    33301 cgtgtttatc acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt
    33361 gaagaactga ctacccgtaa agacgatcag gaagaaaccg tgcgtaaacg tctggttgaa
    33421 taccatcaga tgactgcacc gctgatcggc tactacttca aagaagcgga agcgggtaac
    33481 accaaatacg cgaaagttga cggcaccaag tcggttgctg aagttcgcgc tgatctggaa
    33541 aaaatcctcg gctaatcaag ccgcagcaga tctgctttcg ggcaggtctg ctcatccacc
    33601 tcaggcaata cctctcacag caattagttc ttcttcctca cttttccgct acaattatca
    33661 acaagttgaa tcgataagag gcggtaatgc gtcagactaa aatcggtatc ctgctggcaa
    33721 acctgggtac gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt
    33781 taagcgacag acgcgtggtt gatacctcac ggttgttatg gtggccattg ctgcgcggcg
    33841 ttattttgcc gctgcgctcg ccgcgtgtgg cgaagctgta cgcctcggtc tggatggaag
    33901 gtggctcgcc gctgatggtt tacagccgcc agcaacagca ggcgctggca caacgtttac
    33961 cggagacgcc cgtagcgctg ggaatgagct acggctcgcc atcactggaa agcgccgtcg
    34021 atgaactcct ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct
    34081 cctgttctac ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta
    34141 gcattccggg gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac
    34201 tggcgaacag cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct
    34261 cttatcatgg cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc
    34321 gcacaacgac tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga
    34381 cctttcagtc gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga
    34441 aaatgctcgg agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg
    34501 attgtctgga gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgcgg
    34561 gcgggaaaaa atatgaatat attccagcgc ttaatgccac gccggaacat atcgaaatga
    34621 tggctaatct tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc
    34681 gaagagcctc gtcggcggtt ttcatcatcc gtgaataatg caaaaaggca tgcagcgtac
    34741 ctgggtagag tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac
    34801 ggctgtcatc cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc
    34861 gagtgagatc attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta
    34921 aatacgcctc ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca
    34981 acagacgacg agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg
    35041 caattttacc gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca
    35101 tggcacctgc ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg
    35161 cctgctggtg gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg
    35221 cttcaggtga aagggtgtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca
    35281 ggcgcatgat gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat
    35341 aaaatagcgt cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat
    35401 attttgttgg aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc
    35461 ggcgctcaag cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg
    35521 gtaaatccgg ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa
    35581 caggtagttt gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga
    35641 accactgcca tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg
    35701 ctcccgtcag attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga
    35761 aatttcccgg taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc
    35821 ttcagcaatt ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga
    35881 cgctggtcga tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg
    35941 ccgggcattc actggtgatt gaggatgacg tagccgaagc gctttatcag gagctaaaac
    36001 agaaaaacct gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact
    36061 actcggtgct cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa
    36121 ttggcagtta tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc
    36181 tacaaggcgt ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac
    36241 gtacctttgc tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag
    36301 atgtgattgc cggagcctcg gcactggttc tcacctctta tctggtgcgt tgcaaaccgg
    36361 gtgaacccat gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac
    36421 cggtggtgct gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc
    36481 aattcctcaa agatcacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga
    36541 ccggagaaag cgatccgttg ttggcatctg acaaggcgct ggactgggta gatctggtgc
    36601 tgtgcaccgc cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac
    36661 gtaaaaccca gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta
    36721 gccgcgccat gcgccacaag gattgccaga atccgctgcg tgtctattcg cacattgcgc
    36781 cgtacatggg cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg
    36841 cagcgttgct gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca
    36901 gcaaacataa attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta
    36961 accgtgtgag ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg
    37021 agcgtgaaga cagcctggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg
    37081 ccagataagg cgttcacgcc gcatctggca tttggctctc gatgcctgat gcgacgctgg
    37141 cgcgtcttat catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc
    37201 gtcaccactt cacccgccgg tggcgtttcc agcagttcca gcatagtacg ggcaatttca
    37261 cgttcaccca tcactacctg attcgcgcca cgttcggtga tatacgtcac ttcatcgtca
    37321 taatgggcgc gggcgataat ctcaatatcc gggtttttcg cgcgggcaga cgccacaatc
    37381 tcacccgctt cataaccgtt ggggatcgtc aggatcagcc attttgcaca ttccagatgc
    37441 gccagttgca taatttcttc gttcgctgca ttgcccaata cagcgcggac cccacgctct
    37501 cgcagctcat caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg
    37561 agcaatttct cccccagcag gctgcctaca cgaccgtaac ccaccaatag cgcatggttg
    37621 caaatatcca ctgggatctg tttctcttct tcgattgcct cttccagcgt ctgctcttcc
    37681 agcgtttcgg tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata
    37741 atcgacagga tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat
    37801 gccattccca gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg
    37861 atggttaatg ccgtacgttg ggagtgacca aacagacgga caaggaacag ggccgcgacc
    37921 gatttaccaa acagaataat tgccagcgtc gccagcactg ccagcggttg ctgaatcaga
    37981 attaacggat caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc
    38041 aatggcagcg tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg
    38101 gcaaagaacg cgccaagtgc gaaggagacg tcaaacaact ccaccgcacc aaaggcaatc
    38161 cctaacgcca gcgccagcac cgacagggta aatagctcgc gagaaccggt tgccgcgctg
    38221 cgtgccataa tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg
    38281 atcactttgc cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc
    38341 tgttccatca ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct
    38401 tccacaatca accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct
    38461 tcaagtgcgc gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata
    38521 ccggtcatta acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg
    38581 gctatctggg cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc
    38641 gaaaagtgca aaccgacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc
    38701 gcaagcttgg tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac
    38761 agatatccca ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg
    38821 agcacaaggc cgccaacaat ggtggtgata agc
//
LOCUS       DAJTXQ010000026        38364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000026 DAJTXQ010000000
VERSION     DAJTXQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.026"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            5..81
                     /locus_tag="OO515_001259"
     tRNA            5..81
                     /locus_tag="OO515_001259"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Met,seq:cat)
     gene            130..204
                     /locus_tag="OO515_001260"
     tRNA            130..204
                     /locus_tag="OO515_001260"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:162..164,aa:Gln,seq:ctg)
     gene            241..315
                     /locus_tag="OO515_001261"
     tRNA            241..315
                     /locus_tag="OO515_001261"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:273..275,aa:Gln,seq:ctg)
     gene            complement(469..1644)
                     /gene="ubiF"
                     /locus_tag="OO515_001262"
     CDS             complement(469..1644)
                     /gene="ubiF"
                     /locus_tag="OO515_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6575234.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1790..3214
                     /gene="miaB"
                     /locus_tag="OO515_001263"
     CDS             1790..3214
                     /gene="miaB"
                     /locus_tag="OO515_001263"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6575235.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDMAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3367..4407
                     /locus_tag="OO515_001264"
     CDS             3367..4407
                     /locus_tag="OO515_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6575236.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4404..4871
                     /gene="ybeY"
                     /locus_tag="OO515_001265"
     CDS             4404..4871
                     /gene="ybeY"
                     /locus_tag="OO515_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6575237.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4961..5839
                     /gene="corC"
                     /locus_tag="OO515_001266"
     CDS             4961..5839
                     /gene="corC"
                     /locus_tag="OO515_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6575238.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5864..7402
                     /gene="lnt"
                     /locus_tag="OO515_001267"
     CDS             5864..7402
                     /gene="lnt"
                     /locus_tag="OO515_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6575239.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7480..9357)
                     /locus_tag="OO515_001268"
     CDS             complement(7480..9357)
                     /locus_tag="OO515_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6575240.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9812..10720
                     /gene="gltI"
                     /locus_tag="OO515_001269"
     CDS             9812..10720
                     /gene="gltI"
                     /locus_tag="OO515_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6575241.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10890..11630
                     /gene="gltJ"
                     /locus_tag="OO515_001270"
     CDS             10890..11630
                     /gene="gltJ"
                     /locus_tag="OO515_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6575242.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11630..12304
                     /gene="gltK"
                     /locus_tag="OO515_001271"
     CDS             11630..12304
                     /gene="gltK"
                     /locus_tag="OO515_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6575243.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12304..13029
                     /gene="gltL"
                     /locus_tag="OO515_001272"
     CDS             12304..13029
                     /gene="gltL"
                     /locus_tag="OO515_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6575244.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13147..14082
                     /gene="rihA"
                     /locus_tag="OO515_001273"
     CDS             13147..14082
                     /gene="rihA"
                     /locus_tag="OO515_001273"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6575245.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            14174..15844
                     /gene="hscC"
                     /locus_tag="OO515_001274"
     CDS             14174..15844
                     /gene="hscC"
                     /locus_tag="OO515_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6575246.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15904..17355)
                     /gene="djlC"
                     /locus_tag="OO515_001275"
     CDS             complement(15904..17355)
                     /gene="djlC"
                     /locus_tag="OO515_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6575247.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17352..18059)
                     /locus_tag="OO515_001276"
     CDS             complement(17352..18059)
                     /locus_tag="OO515_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6575248.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            18161..18715
                     /locus_tag="OO515_001277"
     CDS             18161..18715
                     /locus_tag="OO515_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6575249.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18725..20152)
                     /locus_tag="OO515_001278"
     CDS             complement(18725..20152)
                     /locus_tag="OO515_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6575250.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(20149..20856)
                     /locus_tag="OO515_001279"
     CDS             complement(20149..20856)
                     /locus_tag="OO515_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6575251.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            21020..21997
                     /locus_tag="OO515_001280"
     CDS             21020..21997
                     /locus_tag="OO515_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6575252.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(22067..22549)
                     /locus_tag="OO515_001281"
     CDS             complement(22067..22549)
                     /locus_tag="OO515_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6575253.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22784..25366
                     /gene="leuS"
                     /locus_tag="OO515_001282"
     CDS             22784..25366
                     /gene="leuS"
                     /locus_tag="OO515_001282"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6575254.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25381..25962
                     /gene="lptE"
                     /locus_tag="OO515_001283"
     CDS             25381..25962
                     /gene="lptE"
                     /locus_tag="OO515_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6575255.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25962..26993
                     /gene="holA"
                     /locus_tag="OO515_001284"
     CDS             25962..26993
                     /gene="holA"
                     /locus_tag="OO515_001284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6575256.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26995..27636
                     /gene="nadD"
                     /locus_tag="OO515_001285"
     CDS             26995..27636
                     /gene="nadD"
                     /locus_tag="OO515_001285"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6575257.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27660..28271
                     /locus_tag="OO515_001286"
                     /pseudo
     CDS             27660..28271
                     /locus_tag="OO515_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28531..28848
                     /gene="rsfS"
                     /locus_tag="OO515_001287"
                     /gene_synonym="rsfA"
     CDS             28531..28848
                     /gene="rsfS"
                     /locus_tag="OO515_001287"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6575258.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28852..29319
                     /gene="rlmH"
                     /locus_tag="OO515_001288"
     CDS             28852..29319
                     /gene="rlmH"
                     /locus_tag="OO515_001288"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6575259.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29350..31251
                     /gene="mrdA"
                     /locus_tag="OO515_001289"
     CDS             29350..31251
                     /gene="mrdA"
                     /locus_tag="OO515_001289"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6575260.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31254..32366
                     /gene="mrdB"
                     /locus_tag="OO515_001290"
                     /gene_synonym="rodA"
     CDS             31254..32366
                     /gene="mrdB"
                     /locus_tag="OO515_001290"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6575261.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32377..33465
                     /gene="rlpA"
                     /locus_tag="OO515_001291"
     CDS             32377..33465
                     /gene="rlpA"
                     /locus_tag="OO515_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6575262.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33604..34815
                     /gene="dacA"
                     /locus_tag="OO515_001292"
     CDS             33604..34815
                     /gene="dacA"
                     /locus_tag="OO515_001292"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6575263.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34925..35188
                     /locus_tag="OO515_001293"
     CDS             34925..35188
                     /locus_tag="OO515_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6575264.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35289..35930
                     /gene="lipB"
                     /locus_tag="OO515_001294"
     CDS             35289..35930
                     /gene="lipB"
                     /locus_tag="OO515_001294"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6575265.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            36190..37143
                     /locus_tag="OO515_001295"
     CDS             36190..37143
                     /locus_tag="OO515_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6575266.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37352..38317
                     /gene="lipA"
                     /locus_tag="OO515_001296"
     CDS             37352..38317
                     /gene="lipA"
                     /locus_tag="OO515_001296"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6575267.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9617 a         9789 c         9623 g         9335 t
ORIGIN      
        1 atctggctac gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt
       61 cgaatcccgt cgtagccacc aaattctgaa tgtgtcgaat acgttcggca aattcaaaaa
      121 ccaatttgtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
      181 gttcgaatcc tcgtacccca gccaatttat tcaagacgct tacatcgtaa gtgcaccagt
      241 tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc
      301 ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc
      361 attcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg cgacgctgcc
      421 gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct acaaccctaa
      481 cgcatatttc agcgcctgac gtttcaacac gccagcacgc tctgccgcca ttaacccgag
      541 attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa acagatccat
      601 accactttgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct tgaggacagg
      661 ataactggcc cacgcttcac cgtagctgcg ggcattcacc agaacatcaa tcagggcatc
      721 gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg tatgcgcggc
      781 atcgcccacc agcgcaagcc ccggctgcac atactgcaac gcatgtcggc gcgtcagcgg
      841 aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat gcttcgcgat
      901 ttccgcctgg aattgcgcca tattcatatt ctgcaactgg cgaatacgcg ccggagagtc
      961 ataccacacc agcgatgccc agttatcaaa caacggcaga aacgcacgcg gtccgtccgg
     1021 ggtaaattgc tgccaggtgc tgtcgccggg atcgttctca cactgtacgc taatcaacat
     1081 gcacgactgc gcatactgcc aggcatgaac gccaattccc gccatctgcc gcacctgaga
     1141 atttgcgccg tcggcaccaa tcaccagttt cgcgcggatc gtttcaccgc ccttcagttc
     1201 cagctcctga agatcattat ctcgatgtaa tgcaatcagc gagcctggca cacgtaacgt
     1261 tactttcgga tgcgcttcca gcgcctgcca cagcgcctgt tgcaggacgg tgttttccac
     1321 catatagcca agcaacggta gcttaagttc agcggcgtca aacaccacat gcgccgtttc
     1381 ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta ctgcatccca
     1441 gacccctaac cctttaagca atgataccga tgcggcgctg atcgccgaga tccgcacgtc
     1501 cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg ttaccgaaaa
     1561 tccgtgctgt gccagcccca gcgccagcgc gccgccgacc attcctccgc cgacaatggc
     1621 aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc tttaagttta
     1681 caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc agcaaagcat
     1741 tacactatac gccctgcatt cctggctact atttcgcaag agcaagtcga tgaccaaaaa
     1801 actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga agatggccga
     1861 tctgctggat gccacccacg gctatcaact gaccgacatg gcggaagaag cggatgtgct
     1921 gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg
     1981 tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg gtggctgcgt
     2041 ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata ttatttttgg
     2101 gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg accgcagccc
     2161 ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctaccgg aaccgcgcgc
     2221 cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt gcacctactg
     2281 cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata ttctgtttga
     2341 gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc agaacgtgaa
     2401 cgcctggcgt ggtgaaaact acgacggctc caccggatcg tttgccgatc tgctgcgtct
     2461 ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc cgatcgaatt
     2521 caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct tcctgcatct
     2581 gccagtacaa agcggttccg atcgcattct gaacctgatg ggccgtactc acacggcgct
     2641 ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccggatattc agatcagttc
     2701 tgacttcatt gttggcttcc ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct
     2761 gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac gtccgggtac
     2821 accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcaac gtctgtatat
     2881 tctgcaagaa cgtatcaatc agcaagcgat ggcatggagc cgccggatgc tcggcaccac
     2941 ccagcgtatt ctggtagaag ggacttcgcg taagagcatc atggagcttt ccgggcgtac
     3001 cgaaaataac cgcgtggtca acttcgaggg cacaccggat atgatcggta aattcgtcga
     3061 tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc gtactgaaga
     3121 tgaaatgggt ctgcgcgtgg cagaaacacc ggaatcggtg attgcccgta cccgcaaaga
     3181 aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt tccggtgggc
     3241 cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc
     3301 gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac agataagagg
     3361 aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa cgcgcgtctg
     3421 ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg tctcggcatc
     3481 gagatcaatc gccgcgacaa ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct
     3541 gcggcagaca ttctgcgtag cctgtatgtc gatacagccc cgatgcgcgg tcagattcag
     3601 gatatcgaac cggaacagat ccaccttgcg attaaagaag cgcgagtact ggagcaaagt
     3661 gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt
     3721 aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca tgacattacc
     3781 ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc ggcagttgat
     3841 gccctggagc gtcaggagat tcgccgtatt ctgctgactc gtccggcggt cgaagccggt
     3901 gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta tctgcgccca
     3961 ctgtacgacg cgctgtttga aatgctgggc tttgagaaag ttgagaaact gattgagcgc
     4021 aacgttattg aagtcgcgcc gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt
     4081 atcattctcg atgagagcca gaacaccacc atcgaacaga tgaagatgtt cctgacccgt
     4141 atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga cctgccgcgt
     4201 aatactaaat caggcttacg tcacgccatc gaagtgctgg ccgatgtcga agagatcagc
     4261 tttaacttct tccacagcga agacgtggtt cgtcaccctg tggtggcgcg tatcgttaac
     4321 gcctatgaag cctgggaaga agccgaacaa aaacgaaaag cggcgctggc ggcagaacgc
     4381 aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg
     4441 aagataattc cgggttaccg gaagagagcc agtttcaaac atggctgaat gcggtgatcc
     4501 cgcagtttca ggaagaatcg gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca
     4561 gtctgaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg
     4621 aagtgccgcc tggcatggaa atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg
     4681 ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg catatggtgg
     4741 tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa gcagaagaaa
     4801 tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg tacattgccg
     4861 agaaagaata atttgccagg catttgactg gcagacgtcc ccgccgttgg tgattaacga
     4921 cgacgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg acaattcaca
     4981 cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca gccaactttt
     5041 ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt ccgggcagaa
     5101 cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca
     5161 acgcgttcgc gacatcatga tcccccgctc ccagatgatt accctgaaac gcaaccagac
     5221 gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc cggtgattag
     5281 cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc cgtttatgcg
     5341 cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg tcgttcctga
     5401 aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc acatggcgat
     5461 cgtcattgac gaattcggtg gggtttccgg tctggtgacc attgaagaca tcctggaact
     5521 gattgttggt gagattgaag acgagtatga cgaagaagat gatatcgact tccgtcagct
     5581 gagtcgtcat acctggaccg tgcgtgcact ggcttccatt gaagacttca acgaagcgtt
     5641 cggcacccac tttagcgatg aagaggttga cactatcggt ggtctggtga tgcaggcatt
     5701 tgggcatctt ccggcgcgtg gcgaaactat cgacatcgac ggttaccagt tcaaagtggc
     5761 gatggccgac agtcggcgta ttattcaggt tcatgtcaaa atcccggatg actcacccca
     5821 gccgaagctg gatgaataaa accgaaactg gatagataac tacatggctt ttgcctcatt
     5881 aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct gcggaacgct
     5941 ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgttgatgg ggcttcaggc
     6001 gttgaccttt aaccgccgtc cactccagtc tgccgctatt ggcttttgct ggggatttgg
     6061 cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg gcggaatgcc
     6121 tggcccggtt aacatcttcc tggtggtgct gctggcggcg tatttgtcgc tgtataccgg
     6181 actgtttgct ggcgtgctgt cgcgtctgtg gccgaaaacc acctggctgc gcgtggcgat
     6241 tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtac tgacaggctt
     6301 cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc tggcaccgat
     6361 aatgggcgtg gaagccatta acttcctgct gatgatggtt agtggcctgc tggcactggc
     6421 gttggtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg ccctaccctt
     6481 cccgctgcgt tacatccaat ggtttacccc gcaaccggag aaaaccattc aggtttcgat
     6541 ggttcagggc gatattccgc aatcactgaa atgggatgga gatcagcttc ttaatacgct
     6601 gaagatttac tacaacgcaa cggctccgct gatgggcaaa tcatcgttga ttatctggcc
     6661 ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag cactggacgg
     6721 tgagttgcgc gataaaggta gctcgctggt aaccgggatt gtcgacgcgc gtctcaataa
     6781 gcagaaccgc tacgatacct acaacaccat catcacactg ggtaaaggtg caccatacag
     6841 ctacgaatca gccgatcgct ataacaaaaa ccatctagtg ccgtttggcg agtttgtccc
     6901 gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt cgtcgttcag
     6961 ccgtgggcca tatatccagc cgccgctgtc ggtaaatggt attgagctta ctgcggctat
     7021 ttgctacgag atcattctcg gcgagcaagt gcgcgataac ttccgcccgg ataccgacta
     7081 tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat ggcagcactt
     7141 ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa
     7201 cggcattacg gcggtgattg gcccgcaggg ggaggttcag gcgatgatcc cgcagttcac
     7261 ccgcgaggtg ttgaccacta atgtgacacc gaccaccgga ctcacaccgt atgcacgtac
     7321 cggcaactgg ccactgtggg tgctgacggc gctgtttggt tttgccgctg tgttgatgag
     7381 tctgcgagca cgtaaacgtt gatgtgcctg atgtatcctg catgagcaaa acgtactttc
     7441 tcattgagct ttggggcatg gttatcatgc ccctttttct tacggttcag ggatacaagc
     7501 cacattgcca tattggggat gacgaataat tgcctgcgga tcaagcgagc gcgtcctgtc
     7561 catttggatc aacccgttct tcgccatgac aaatattttt ctaccatccg ctgaaaaacg
     7621 aaggtgatta acgtagctgg caggataaac gccaataatc tgcccggcat taatattgag
     7681 caggatgaca taaccttgca ggccatatga caccaacgct aaatattgac cgtccgggct
     7741 aataccctca acggaataaa cgctcctgct acaccattgc gtcataatgc tggcttgttg
     7801 atcccctacg cttatacgtg aaatcccctg actatcggca aaaataacct cgccgttttc
     7861 aggtcgaaaa cgcatcgccg atatcgttcc acgcgcatca cctgcgggaa agtcttcttt
     7921 ctgatgcgtg atggtattga tccgcacgat gttacctctg cctgctcccg gaaccagtaa
     7981 tgttctttca tccggggaaa gtgccataac ccaggcgcct ggtttaccgc tcgtccccgt
     8041 cccgtcgtat ttctccagcg ggatatccgc cacatgctga taagtcacca gatcaatcgc
     8101 tggcacacta ttactggtgc gattcatcac ccaggctttt gtgccatcac gcgagaggat
     8161 aaggctgtca ggatgtgcac cggtggtaat cgactgaata atctcccctg tcgccacgtt
     8221 aatgaccgat aacgcattcg cttcaaatga agtcgcataa atcaactttt catccgggct
     8281 taatgccagc gatctgacgc cctgatcttt acagccacca caatgtttga ctgacttttc
     8341 tttcgctatg ggcgctgtaa aggtatgcag gattttcttt tgtccgaggt cgaaaacaac
     8401 aatgctgttc gtgttttcaa ttgcggcata caacatatta ccatcccgac tgatcgccat
     8461 atcatggata tcggcaatct cagcgagcaa atcaccactg gcctgttcac gacttaccgg
     8521 agcggataat gtttccctga gggcgttgcg ttcctccgct aactgctgct ttttttgccg
     8581 tcgctcttgt tcaatctcct gtggataaaa cgcttcacgc aggctttgcc tgacctgtaa
     8641 aactgactca tccatctgct gctgcgcaag ccagatagcc ccggtcagaa gaagactgcc
     8701 cgcaacaata atcccgcctt ccgtaacgag tcggttttgc ccagataaac gcgcgctcag
     8761 ccaacccaac gcgccacccg caatcaggcc gccaatgtgg caagcgttat ctattcctgt
     8821 ttgccgggca ccgtacaaca gtgtcagcgc caccataccc aacagaggaa atactcgccg
     8881 ctggttttta tggtatgtgc ccgtacctga agcaaactgt gttgctatcg ctgcaccagc
     8941 tattcccaga atcgccccgg atgccccaac gctgatgact atctgctcac tttcgcgtaa
     9001 cgcataacag gcgctgatga gcccaccaaa gacgccggag aataaccagg taatcaggag
     9061 tcgccatttc cccagaatct gttccgccac gcctcccacg accaggaatg caaacatatt
     9121 catcagcaga tgagaaacac tactgtgcag aaagatactg ctcaataagc gccagggttc
     9181 acccgacaaa gtaagaggcg caacgtttgc gccgagatgg ataagtgcgg attgatcgac
     9241 ctcttcataa ctcatccatt gtgcgagata acaaaatatc ccaatacaga gtgcaaaaag
     9301 gataagggtt attgcgggta attgaacatt taacggctta accgaagatg cagacataag
     9361 gtgtttcttc cttgaaaaga caggtaaaaa gcatttttcc cgtgtgcagt tatcatcccg
     9421 gcaaacgtcc catgccgaaa agataattct gtccgaaata tgactttttc agcctaaaat
     9481 tacaatcgta attaattcgc ccctaagtat tttttggcat gtaagttgct ttatttactg
     9541 tgtaagcgat gaaatcagcc gcgacgcaac caggttgcac caccacaaat cagcggtagc
     9601 accaaaaagg tgcggtttaa gaattttgct tcggaatggt gcggcgcatc tcgcaaaaac
     9661 aaacaataaa acagcaatat ctttacattt agccaaacta aatgttacct aacaggcaca
     9721 acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg gtatccatgc
     9781 gttcttaacg cagaagataa aggagttgga tatgcaatta cgtaaacctg ccacagcaat
     9841 cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc cggcagcagg
     9901 cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc
     9961 agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc aggattactc
    10021 caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact
    10081 gattccgatt acctcacaaa accgtattcc actgctgcaa aacggcactt tcgattttga
    10141 atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct ctgatactat
    10201 tttcgtggtc ggtacgcgcc tgctgaccaa aaagggcggc gatatcaaag attttgccga
    10261 cctgaaagac aaagccgtag tcgtcacttc cggcactacc tctgaagttt tgctcaacaa
    10321 actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc acagtgactc
    10381 tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgatg ccctgctggc
    10441 cggtgaacgt gcgaaagcga agaaaccaga caactgggaa atcgtcggca agccgcagtc
    10501 tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa agctgatgga
    10561 tgacaccacc gcgcaggtgc agacctccgg tgaagcggaa aaatggtttg ataaatggtt
    10621 caaaaatccc attccgccga aaaacctgaa catgaatttc gaactgtcag acgaaatgaa
    10681 agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac caggggcgga
    10741 aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga tggtcgttcc
    10801 ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag cagttaacgc
    10861 tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg ggtatttttt
    10921 tacaacaagc cccgttcggc aacaccacct atcttggttg gatctggagt ggttttcagg
    10981 tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg
    11041 gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg tatgtcgaac
    11101 tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg atcccggagc
    11161 tgttgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat attcagtttt
    11221 tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac
    11281 gcgccgccat tcagtcgttg ccgcgtgggc aaaaaaatgc cgcgctggcg atgggcctga
    11341 cgctaccgca ggcttatcga tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc
    11401 cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg actattggtc
    11461 tggtggatat ggcggcgcag gcgggtaaat tgcttgatta ctcagcccac gcctgggagt
    11521 cgtttaccgc tattacgctg gcgtatgtgt tgattaacgc ggttatcatg ctggtaatga
    11581 cgctggttga acgtaaagtt cgcctgcctg gcaacatggg gggcaaataa tgtacgagtt
    11641 tgactggagt tccattgtcc cttccctgcc atatctgctc gacgggctgg tgatcacgct
    11701 gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggcaccatgc tggcggtgat
    11761 gcgcttgtcc agctttgcgc ccgtagcgtg gtttgccaaa gcctacgtta acgtattccg
    11821 ctcgattcct ttagttatgg ttttgctgtg gttttacctg atcgtgccag gttttctgca
    11881 aaacgtgctg ggattatcac caaaaaatga tatccgttta atttcggcga tggtggcgtt
    11941 ttcgatgttt gaagcggcct actattcaga gattatccgt gctggtattc aaagtatttc
    12001 tcgtggtcag tcgagcgccg cgctggcgtt gggaatgact cactggcagt cgatgaaact
    12061 gattattctg ccgcaggcgt tccgcgcgat ggtgccgctt ctgctcactc agggcatcgt
    12121 actgttccag gatacctcac tggtgtatgt gttatctctg gccgatttct tccgtaccgc
    12181 ctcaaccatt ggtgagcgtg atggtacgca ggttgagatg atcctgtttg ccggatttgt
    12241 ttatttcgtt attagtctta gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc
    12301 ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt gctgaccgac
    12361 tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc tggttccggc
    12421 aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg tgaaatcacc
    12481 gtcgatggta tcgtggttaa tgacaagaaa accgatctgg caaagctgcg ttcccgcgtc
    12541 gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga aaacctgacc
    12601 ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa agcattgaaa
    12661 ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca gctttccggc
    12721 ggtcagcagc agcgtgtggc aatcgctcgc gcattgtgta tggatcctat tgcgatgctg
    12781 ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct ggacgtgatg
    12841 gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat gggctttgcc
    12901 cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga agactcgccg
    12961 aaagacgctt tcttcgatga tccgaaatcg gaccgcgcga aagacttcct cgcgaaaatc
    13021 ctgcattaat cactctggcg cgcataccca agtgcgcgcc atctcacgct ttatcgtttc
    13081 gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac acaacaagga
    13141 gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgacga cgctatcgca
    13201 atagttctcg ccctcgcctc accagagctt gatgtcaaag caattacgtc ttccgccgga
    13261 aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt gcttaatcgc
    13321 accgatattc cggtagcagg cggcgcggta aaaccgttaa tgcgtgagtt gattatcgcg
    13381 gacaatgtgc acggcgaaag cggtctggat ggcccggcat taccggaacc gacgttcgca
    13441 ccgcaaaact gtacggcggt agagctgatg gcgaaaacgc tgcgcgaaag tgcggaacct
    13501 gtcacgattg tgtctaccgg accgcaaact aacgttgcct tgctgctcaa tagccatccg
    13561 gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac
    13621 tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga aattgtcttc
    13681 cagtcaggga tcccagtggt gatggccggt ctggatgtta ctcacaaagc acaaatccac
    13741 gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa
    13801 ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt cggtgcaccg
    13861 ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac cactgttgag
    13921 cgctgggttg gcgtggaaac acaggggaaa tatagccagg gtatgacggt tgttgattat
    13981 tattatctga ctggcaataa accgaatgcc actgtaatgg tcgatgttga tcgtcagggc
    14041 tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacacacgtc attgattaag
    14101 atcaccacgc gttacctggg tggtcttaat ctgtttatct gtttatctct ttttctttca
    14161 agatataaat gcaatggata atgcagaact cgccattggt atcgatctcg gcactaccaa
    14221 tagtttaatt gccgtctgga aagacggtgc cgcgcaatta attccaaata agttcggtga
    14281 atatttaaca ccatccataa ttagcatgga tgaaaataat catattttag tcggaaaacc
    14341 ggctgtatca cggcgtactt cgcatccgga taaaacggca gcgttattta aacgtgcaat
    14401 gggcagtaat accaactggc ggttaggcag cgacacattt aacgcgccag aactgtcctc
    14461 tttggtatta cgctcattaa aagaagatgc cgaagaattt ctgcaacgtc cgattaaaga
    14521 tgtggtgatc tccgttccgg cttatttcag cgatgaacaa cgcaagcata cccgtttagc
    14581 agcggagtta gccgggttaa atgcggtacg cttaattaat gaacccacag cagctgcgat
    14641 ggcatatggc ctgcatacgc aacaaaatac ccgctcgctg gtttttgatc tcggtggcgg
    14701 cacgtttgac gtcacggtgc tcgaatacgc cacgccggtg attgaagttc acgcctccgc
    14761 tggcgataac ttcctcggtg gcgaagattt tacccatatg ttggtcgatg acgttttaaa
    14821 gcgcgcggat gtcgccagga ccacgcttaa cgagagtgaa ctggcagcct tgtacgcctg
    14881 tgtggaagcg gcaaaatgta gcaatcaatc gccattgcac attcgctggc agtaccagga
    14941 tgaaacgcgg gagtgcgaat tttacgagaa cgaactggaa gatttgtggt tgccgctgct
    15001 caatcgcttg cgagtgccga ttgaacaggc gttgcgcgat gcacgtctga agccgagtca
    15061 aatcgacagt ctggtgctgg ttggcggcgc gtcacaaatg ccgctggtgc agcgaatcgc
    15121 cgtgcgtctg tttggcaaat taccgtatca aagttacgac ccgagcacca ttgtcgcgct
    15181 gggcgcagca atccaggccg cctgccgctt acgcagtgaa gatattgaag aggtgatcct
    15241 cactgatatt tgcccttact cgttgggcgt tgaagttaac cgccagggcg tttccggcat
    15301 tttctcgcca attattgaac gaaacaccac agtacccgtg tcgcgtgtag aaacttactc
    15361 gaccatgcac ccggaacagg attctattac ggttaacgtc tatcagggag aaaaccacaa
    15421 agttaaaaat aacattctgg ttgaatcctt cgatgtgccg ttgaagaaaa ccggggctta
    15481 tcagtcgatt gatattcgct ttagttatga tatcaacggc ttgcttgaag ttgacgtgct
    15541 tctggaagat ggcagcgtta agtccagagt cattaatcat agcccggtaa cattgagcgc
    15601 gcagcagatt gaagagagtc ggacgcggtt atccgcattg aaaatttatc cgcgcgatat
    15661 gctaatcaac cgcaccttta aggccaaact ggaagagttg tgggcgcggg cgctgggtga
    15721 cgagcgagaa gagattggcc gggtgatcac cgattttgat gcggcgttgc agtcaaacga
    15781 tatggcccgc gtcgatgaag ttcgtcgacg ggcgagcgat tatttggcta ttgagatccc
    15841 ttaaacacgg ctgccggatg cggcataaac gctgcatccg gcatcggcgc aaaaaacaca
    15901 tcctcagcta aacaacatcg cgatttttcc gagcgcataa cctatcatca gcaccgttaa
    15961 gctgattttg ataatactgg taatgctctg tggtttttca tcctcatcat ttttttcttc
    16021 atggttttca ggtgttggct catcgacatc ttcatcctcc ggctcgagtt tcactttcgt
    16081 caacagcatc tgggtgataa tggcctctaa tggatgagca tctccacctt ccagccttgt
    16141 attaagacgg gccagcatct cttgtttcgg gaacgcttcc agcacaacct gttccgctac
    16201 ccgatacaaa tccagtaacg gtgaatcttg ctctgtctcg ctaccgactc tcgatttcca
    16261 ccacgcccat gctttgcgct tgaagaataa cgactcaata aacgattgac ctaacggcga
    16321 taattttccg ctatgtaaaa tctccgccca taacagccgc gtccgggcgc tctcaccagg
    16381 aacatatttc aacggtttac cttgcgcatc taccatgctt cgcgcttcga taaccatcac
    16441 taataacgcg aaataatccg ggcaatgacg cttgctccac tgcaacaaca aattatcagc
    16501 ttgtgtagat gggtaacttt cccagtacgc gcagagatcg gcaagcaggc tttccccctc
    16561 gccgcagtac aaacgttgtg cacactggta ataccgggca tcttcgcttt ctggttcttc
    16621 cgcttgccac tgtttggcta caggaatgag ttccgccata cctgtttgca ccgagctgta
    16681 ccagcgcaac agtttgcgat gtaactttgc atcatcagga atgaaccacg gtccttccat
    16741 cgccaaaaac gcagtgacat agtcaggatg gtaacggcag caacgttcca gcgcgaagta
    16801 atattcaaca gtctggttct gtaacgcgac gggatattcc agaaggctaa ggaaatcgaa
    16861 acaatcacca gcctgaatgg cctcaagaaa agcctccctt tcttcctcat ccattccttc
    16921 gctgtcatta aacgaatgcc agttcaaacg ctctgccaac aaagaagcac aatttaatga
    16981 aagatatcgc gcttctatag cgattgcaca cagcggccag cgtaattgat cgacatcctc
    17041 catgttccag gtatttaatt gctgaataaa tttctgccag gcggagggct gaaaacgatc
    17101 actttctgaa tccagtaatg tcctgaatgc acgtagtatt tcatgttcag cggtggcatc
    17161 tttttcttca tcatcggctt cctcgacagg atttaccgcc agtcgcagcg cctcttcata
    17221 ggcctggcgt aatgctttaa acccctgcgg atcggtttcg ggatgacaca acggtaagcg
    17281 agcaagataa gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt cgaggatttg
    17341 ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta ccgggcattc aggctggaca
    17401 gttaatggtt gccagggaag cgtggcatag aaacggtcgc ttcccgctgc cagctcttcg
    17461 aacatccgac tcccggcgct atcgtatttg tacagggtta gcgcctcgcg tagcgaagcg
    17521 ttgtcttcgc actgagaaga ttgccagtag agacgcccta gtgaataggc agaaaaatag
    17581 tgcatccagc tgtgatagta atggtgagct cgtgcataga cgcgcgattg cagccataaa
    17641 ctctcttctt ccgtcagcca gttgttgagc acaccgaccc gtgacagaaa ccccattcga
    17701 acataatccc aggctttgat tcccccttct ccacagcaca tagcggtttg ggcaacgaat
    17761 tgggtataaa tcaaccctct ctccgagcca ccagcaatta atgctttcct ctcttcaggg
    17821 gaagagcgtt gccatgtcag atataagcca gctaaatcat tggcatgacc atcatcagtc
    17881 atctgataaa tagtctgaat taaatcatca cgattattta ttccccagga tgatgagaga
    17941 tcgaattctt ttatcccagt atacattttt ggcgagtcat gcgcgggttt gcaactctga
    18001 ttaagtattt ccatgggcgc agaaagcgca aaaagaagat attgttcttc cttattcatt
    18061 ttttaataat cccttaagtg cattaactga ctactttttt attattgatg agtataaact
    18121 tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac atgaataaaa aatcaatgta
    18181 tatattcgca atttttatag ttgcagcaat tacctgtatt agccaaccca agaaaacgac
    18241 gttgcgtgat aaagccatgg tgaattatgc ctttgattat ttaagatcac caggcagtct
    18301 accgttaacc acggcagcca cggagctttc cgcgattcat ggtcactcaa catcgcaata
    18361 tcgccttggc gaattttatc ttcatggtag cgacggtaaa ccactggatt atacacaggc
    18421 gagatactgg tatgagcaat cagcagaaca ggaaaatccg cgcgcacaaa gcaaactggg
    18481 gtggatctac ctgaaaggcc tgggagtcaa acccgatacc cgtaaagcaa ttctctggta
    18541 taaggaagca gctgaacaag ggtatgctca tgctcaatat actttgggtt tgatctacag
    18601 aaatggcact ggtattaatg ttaaccatta tgaatctcaa aaatggttaa aactggccgc
    18661 caaacaacat tacaaaaatg cggaaagatt acttgccgga cttcccgcac attaacgtcg
    18721 actgtcaccc gaagagatgg agtatttttc ctatcaaacc ggcaaagata aaaatgtaga
    18781 aaataatttt gatgacgccc agacagccca gcccgccatc gtcaacgggc attggctgcg
    18841 gttgcggctc cggctcgacg gtttcctgac cggtaaaatt cgccgcctga attaataatc
    18901 gacgaataat taatgcttcc agtggacaac gatcttctgc atcaaggcgg ctcacaatcg
    18961 gcattaatga ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata
    19021 atggccataa gggatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt
    19081 gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt
    19141 cgtataaatg aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct
    19201 gatcgttaac cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc
    19261 gatgctgacg gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc
    19321 agtagtcaca aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt
    19381 attcataata acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat
    19441 ccagaagttc ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca
    19501 gttttgcatc atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt
    19561 ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca
    19621 cggcaacggg cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat
    19681 ataaaaagga ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc
    19741 gcaatctttc tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga
    19801 ttgtgcacag cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct
    19861 gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc
    19921 gaaaggcgag cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt
    19981 tagcaggcga ctgcgcaatc cggagcgcct cctcatacgc ctgacgaagt tgtttaaaac
    20041 cctgcggatc ggtttctgga tgaaaggaca gtaacagcgc cagataagcg cggcggataa
    20101 tatcgacgtc agtcgtttcc tctatttcga ggatcttcca gcaatttttc ataggtcgct
    20161 catatcctta agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggc
    20221 agggtaatac gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc
    20281 gtattcttta cgagctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg
    20341 ccaatattgc cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg
    20401 tagagcccgt agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt
    20461 attaagtacg cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc
    20521 tccctcacca cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc
    20581 atttagttca gcaagatagt cacgccattc acatggcgaa taacgaaacc agcggtgata
    20641 aaaccctgcc aaccgagcgg catgaccgtt atcgatcatt cggtgaattg tgcgcagcag
    20701 gtcatcacgg tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata
    20761 cattttgggg gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga
    20821 aagtgcaaac aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt
    20881 taatgaatgt aaactacatt tcgagactca gttatttata cttttctgac gcaacataat
    20941 acgcataaaa taaggcttac gcaaaaatgg aacacataaa aatataatat gttttatcta
    21001 caaccataag gaaatgataa tggtttttac gtcaagttgc tgcgataatt tatcaataga
    21061 tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct caatatattg ttgggtttta
    21121 ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa aaggcctttt actggctgaa
    21181 gctggccgct gagcaaggtc attgtgaagc acagtattcc ttagggcaaa agtataccga
    21241 ggataaaagc cgtcataaag ataatgagca agccatcttc tggctgaaaa aagctgccct
    21301 acaaggccat actttcgctt ccaacgccct tggctggaca ctggatcgtg gagaagcccc
    21361 caattataaa gaagcggttg tctggtatca gatagccgcg gagagcggaa tgtcttatgc
    21421 gcaaaataat cttgggtgga tgtacagaaa tggcaacgga gtcgcaaaag actatgcgct
    21481 ggcatttttc tggtacaaac aagctgcatt acaaggccat agtgacgcgc aaaacaatct
    21541 ggccgatctt tatgaagacg gaaaaggcgt tgctcaaaac aagacactcg ccgcattctg
    21601 gtatttgaaa agcgcacagc agggtaatcg gcacgcccag tttcaaattg catgggatta
    21661 taacgctggc gaaggggtgg accaggacta taagcaagcg atgtactggt atctgaaggc
    21721 tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt tatatgtata aacacggaca
    21781 aggcgttgag aaagattatc aggctgcctt tgaatggttt acgaaagccg ctgaatgcaa
    21841 tgacgccact gcctggtata acctggccat tatgtatcac tacggagaag gaagacctgt
    21901 cgatctccga caggctctcg acctgtatcg taaagttcag tcatccggaa ccagagatgt
    21961 cagtcaggaa attcgtgaga ctgaagattt actgtagaga taaaaacaag ggccggggaa
    22021 tttcattcgg cccatattct ctttattgct gtgcgaaatc agactattac ggctcaaacg
    22081 ggcgtctctg gaactggtca tgaccacatt tcgggcatag cgtcagcact tccggtgtgt
    22141 agatcgggag atggaagtga catttctcgc agaccagatt tcccagcccg accacttctc
    22201 cgctgtgata aaccccatga tgattgaggt cctggaaaac ttcgcgccat tcaagctgcg
    22261 ttttatcggt gatatctgcc agctcctgcc ataagctttc tttaatcacc cgcataaaga
    22321 cgctgtcaga ttcttctttc aggctctctt catagctcat ggcgaactct tccaggtcac
    22381 gtcttacagc tcgcgtcagc tcatcgacct cggttcgcgt taactcccct gtttttatta
    22441 cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg ttcgccattg cgcaggcgtt
    22501 cgctcagtga cgcgaccagt tcacggtaat attgagcaac cttgttcatc gtttcatctc
    22561 ccgggtcgtt aactgactat aataatagac gttctttgtt ccttgctttg ctaatacgcc
    22621 cacaaactct gtaaattaat gtatgaataa tccgggcatc atttcaacgt cgagtgcgcg
    22681 aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg gatctgaaaa accacctcaa
    22741 cgctacattt gtagccgtat tgaaaacagg accactggct gccatgcaag agcaataccg
    22801 cccggaagag atagaatcca aagtacagct tcattgggat gagaagcgca catttgaagt
    22861 aaccgaagac gagagcaaag agaagtatta ctgcctgtct atgcttccct atccttctgg
    22921 tcgactacac atgggccacg tacgtaacta caccatcggt gacgtgatcg cccgctacca
    22981 gcgtatgctg ggcaaaaacg tcctgcagcc gatcggctgg gacgcgtttg gtctgcctgc
    23041 ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg tggacgtacg acaacatcgc
    23101 gtatatgaaa aaccagctca aaatgctggg ctttggttat gactggagcc gcgagctggc
    23161 aacctgtacg ccggaatact accgttggga acagaaattc ttcaccgagc tgtataaaaa
    23221 aggcctggta tataagaaga cttctgcggt caactggtgc ccgaacgacc agaccgtact
    23281 ggcgaacgaa caagttatcg acggctgctg ctggcgctgc gataccaaag ttgaacgtaa
    23341 agagatcccg cagtggttta tcaaaatcac tgcttacgct gacgagctgc tcaacgatct
    23401 ggataaactg gatcactggc cagacaccgt taaaaccatg cagcgtaact ggatcggtcg
    23461 ttccgaaggc gtggagatca ccttcaacgt taacgactat gacaacatgc tgaccgttta
    23521 cactacccgc ccggacacct ttatgggttg tacctacctg gcggtagctg cgggtcatcc
    23581 gctggcgcag aaagcggcgg aaaataatcc tgaactggcg gcctttattg acgaatgccg
    23641 taacaccaaa gttgccgaag ctgaaatggc gacgatggag aaaaaaggcg tcgatactgg
    23701 ctttaaagct gttcacccat taacgggcga agaaattccc gtttgggcag caaacttcgt
    23761 attgatggag tacggcacgg gcgcagttat ggcggttccg ggtcacgacc agcgcgacta
    23821 cgagtttgcc tctaaatacg gcctgaacat caaaccagtt atcctggctg ctgacggctc
    23881 tgagccagat ctttctcagc aagccctgac tgaaaaaggc gtgctgttca actctggcga
    23941 attcaacggt cttgaccatg aagcggcctt caacgccatc gccgataaac tgactgcgat
    24001 gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac tggggtgttt ctcgtcagcg
    24061 ttactggggc gcgccgattc cgatggtgac gctggaagac ggtaccgtaa tgccgacccc
    24121 ggacgaccag ctgccggtga tcctgccgga agatgtggtg atggacggca ttaccagccc
    24181 gattaaagca gatccggagt gggcaaaaac taccgttaac ggtatgccag cgctgcgtga
    24241 aaccgacact ttcgacacct ttatggagtc ctcctggtac tatgcgcgct acacttgccc
    24301 agagtacaaa gaaggtatgc tggattccga agcggctaac tactggctgc cggtggatat
    24361 ctacattggt ggtatcgaac acgccattat gcacctgctc tacttccgct tcttccacaa
    24421 actgatgcgt gatgcaggca tggtgaactc tgacgaacca gcgaaacagt tgctgtgtca
    24481 gggtatggtg ctggcagatg ccttctacta tgttggcgaa aacggcgaac gtaactgggt
    24541 ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc cgtatcgtga aagcgaaaga
    24601 tgcggcaggc catgaactgg tttataccgg catgagcaaa atgtccaagt cgaagaacaa
    24661 cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg gacaccgttc gtctgtttat
    24721 gatgtttgct tctccggctg atatgactct cgaatggcag gaatccggtg tggaaggggc
    24781 taaccgcttc ctgaaacgtg tctggaaact ggtttacgag cacacagcaa aaggtgatgt
    24841 tgcggcactg aacgttgatg cgctgactga agatcagaaa gcgctgcgtc gcgatgtgca
    24901 taaaacgatc gctaaagtga ccgatgatat cggccgtcgt cagaccttca acaccgcaat
    24961 tgcggcgatt atggagctga tgaacaaact ggcgaaagca ccaaccgatg gcgagcagga
    25021 tcgcgctctg atgcaggaag cgctgctggc cgttgtccgt atgcttaacc cgttcacccc
    25081 gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa ggcgatatcg acaacgcgcc
    25141 gtggccggtt gctgacgaaa aagcgatggt ggaagactcc acgctggtcg tggtgcaggt
    25201 taacggtaaa gtccgtgcca aaatcaccgt tctggtggac gcaacggaag aacaggttcg
    25261 cgaacgtgct ggccaggaac atctggtagc aaaatatctt gatggcgtta ccgtgcgtaa
    25321 agtgatttac gtaccaggta aactcctcaa tctggtcgtt ggctaagcgc gggaggaagc
    25381 gtgcgatatc tggcaacatt gttgttatct ctggcggtgt taatcaccgc cgggtgtggc
    25441 tggcatctgc gtgataccac gcaggttcct tccactatga aggtcatgat cctggactca
    25501 ggcgatccga acgggccatt aagccgtgca gtgcgtaacc agttacgtct gaatggtgtc
    25561 gagttgcttg ataaagaaac cacgcgtaag gacgttccat ccctgcgttt gggtaaagtg
    25621 agcatcgcga aagataccgc atcggtattc cgtaacggtc aaacagcaga gtatcagatg
    25681 atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg atatctaccc gattagcgcc
    25741 aaagtcttcc gttcgttctt tgataacccg caaatggcgt tagcgaaaga taacgaacaa
    25801 gacatgatcg taaaagagat gtacgaccgt gctgccgaac agctgattcg taagctgcca
    25861 agcatccgtg ctgcggatat tcgttccgac gaagaacaga cgtcgaccac aacggatact
    25921 ccggcaacgc ctgcacgcgt ctccaccacg ctgggtaact gatgattcgg ttgtacccgg
    25981 aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc gtatctttta cttggtaacg
    26041 atcctctgtt attgcaggaa agccaggacg ctgttcgtca ggtagctgcg gcacaaggat
    26101 tcgaagaaca ccacactttt tccattgatc ccaacactga ctggaatgcg atcttttcgt
    26161 tatgccaggc tatgagtctg tttgccagtc gacaaacgct attgctgttg ttaccagaaa
    26221 acggaccgaa tgcggcgatc aatgaacaac ttctcacact caccggactt ctgcatgacg
    26281 acctgctgtt gatcgtccgc ggtaataaat taagcaaagc gcaagaaaat gccgcctggt
    26341 ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca gacaccggag caggctcagc
    26401 ttccccgctg ggttactgcg cgcgcaaaac agctcaactt agaactggat gacgcggcaa
    26461 atcaggtgct ctgctactgt tatgaaggta acctgctggc gctggctcag gcactggagc
    26521 gtttatcgct gctctggcca gacggcaaat tgacattacc gcgcgttgaa caggcggtga
    26581 atgatgccgc gcatttcacc ccttttcatt gggttgatgc tttgttgatg ggaaaaagta
    26641 agcgcgcatt gcatattctt cagcaactgc gtctggaagg cagcgaaccg gttattttgt
    26701 tgcgcacatt acaacgtgaa ctgttgttac tggttaacct gaaacgccag tctgcccata
    26761 cgccactgcg tgcgttgttt gataagcatc gggtatggca gaaccgccgg ggcatgatgg
    26821 gcgaggcgtt aaatcgctta agtcagacgc agttacgtca ggccgtgcaa ctcctgacac
    26881 gaacggaact caccctcaaa caagattacg gtcagtcagt gtgggcagag ctgaaagggt
    26941 tatctcttct gttgtgccat aaacccctgg cggacgtatt tatcgacggt tgatatgaaa
    27001 tctttacagg ctctgtttgg cggcaccttt gatccggtgc actatggtca tctaaaaccc
    27061 gtggaaacgc tggcgaattt gattggtctg acgcgggtca caatcatccc taataatgtt
    27121 cctccgcatc gtccccagcc ggaagcgaac agcgtgcagc gtaaacacat gcttgaactg
    27181 gcgattgccg acaagccatt atttactctt gatgaacgcg agctaaagcg caatgccccc
    27241 tcttacactg cgcaaacact gaaagagtgg cggcaggaac aaggaccgga cgtgccgctg
    27301 gcgtttatta ttggtcagga ttcactgctg acctttccga cctggtacga atacgaaacg
    27361 atactcgaca atgcacattt gatcgtctgt cggcgtccag gttacccact tgaaatggcg
    27421 caaccgcaat accagcaatg gctggaagat catttgacac ataacccgga agatcttcac
    27481 cttcagcctg ccggtaaaat ttatctggct gaaacgccgt ggtttaacat ctcggcgacc
    27541 atcatccgcg aacgtttgca aaacggtgaa tcgtgtgagg atttattgcc ggaaccggta
    27601 ctgacttaca ttaaccaaca aggcttgtat cgctgatacc tgtcgttacg gagggcatga
    27661 tgcgactgtg gttaattcgt catggtgaaa cgcaagcgaa tatcgatggt ctttacagcg
    27721 gtcatgcgcc cacccccctg accgcgcgcg gtattgagca agcgcaaaat ctgcatacgc
    27781 tgctacatgg tgtttccttt gatctggttt tatgcagtga actggaacgg gcacagcata
    27841 ccgcacgact ggttctcagt gaccgccagc tccccgtgca aatcatccct gaactcaacg
    27901 aaatgttttt tggcgactgg gagatgcgac atcatcgcga cctcatgcaa gaagatgccg
    27961 aaaactatag cgcgtggtgc aatgactggc agcatgcaat ccccacgaac ggtgaaggat
    28021 ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag gcttagtgaa tttcagcact
    28081 atcagaatat tttagtcgtc agccatcagg gtgtactgag tctgttaatc gcccgtttaa
    28141 ttggcatgcc tgccgaggct atgtggcatt ttcgcgttga ctaggggtgt tggagcgcca
    28201 ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa tagccgtgcc atcggggtcg
    28261 agaatgcatg acttttctgt ttttttacgg gtaagccgca acgaccattg acagacccgg
    28321 gcaggctgat attctccgca gccagacttt tttcgccaga cacgactttg tagaaattgt
    28381 tttacaaaaa tggcgatgca atctgcggcg cggggtggga tgatagccca ctttcgaaag
    28441 ccgattcggc gacaattgtc ccgaaatcgc ctctggttca ggtatactga cagaccattt
    28501 ttatctattt gattcaccca gggggaaaac ttgcagggta aagcactcca ggattttgtt
    28561 atcgacaaaa ttgatgacct caaaggtcag gacatcatcg ccttagacgt tcaaggcaaa
    28621 tccagcatca ccgactgcat gatcatctgt acgggtacgt ccagccgtca tgttatgtcc
    28681 attgctgacc acgttgtgca ggagtctcgc gcagcgggcc tgttaccgct cggcgtagaa
    28741 ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg atgtgattgt ccatgtcatg
    28801 caggaagaga gccgtcgcct gtatgaactg gaaaaactct ggagttaatg cgtgaagctg
    28861 caacttgtcg ccgtgggaac gaaaatgccg gactgggtac aaaccggttt taccgagtac
    28921 ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg aaattccggc cggaaaacgc
    28981 ggcaagaatg cggacatcaa gcgcatactc gacaaagagg gtgagcagat gttggcggcc
    29041 gcaggcaaaa accgcattgt caccctcgat attccaggca agccctggga tacgccgcag
    29101 ttagccgctg agctggaacg ctggaagctg gatggtcgcg acgtcagtct actgattggc
    29161 gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg agcagagctg gtcgctgtcg
    29221 gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg cagagagtct gtaccgggcg
    29281 tggagcatca ccaccaacca tccttatcac cgtgagtgat aagggagctt tgagtagaaa
    29341 acgcagcgga tgaaactaca gaactctttt cgcgactata cggcagagtc cgcgctgttt
    29401 gtgcgccggg cgctggtcgc ctttttgggg attttgctgc tgaccggcgt gcttatcgcc
    29461 aacctgtata atctgcaaat tgttcgcttt accgactacc agactcgctc taatgaaaac
    29521 cgcattaagc tggtgcctat cgcgcccagc cgcggcatta tctacgaccg taatggtatc
    29581 cctctggccc tcaaccgcac tatctaccag atagaaatga tgccagagaa agtcgataac
    29641 gtgcagcaaa cgctggacgc tttgcgcagc gtggttgatc tgaccgatga cgatattgct
    29701 gcattccgaa aagagcgcgc acgttcacac cgtttcacct ctattccggt gaaaactaac
    29761 ctgaccgaag tacaagtagc tcgctttgcc gtcaatcagt accgttttcc gggtgtcgaa
    29821 gttaaaggct ataaacgtcg ttactatcct tacggttcgg cgttgaccca cgtcatcggc
    29881 tatgtgtcga aaatcaacga taaagacgtc gaacgcctga ataatgacgg caaactggcc
    29941 aactatgcgg caacgcatga tatcggtaag ctgggcattg agcgttacta tgaagacgtg
    30001 ctgcacggtc agaccggtta tgaagaggtt gaagttaaca accgtgggcg tgttatccgc
    30061 cagttaaaag aagtaccacc gcaagccgga cacgatattt acctgacgct ggatctcaaa
    30121 ctccagcaat atattgaaac gctgctggcg ggtagccgcg cagctgtggt tgtcaccgat
    30181 ccgcgtacag gtggggtgct ggcgctggtt tccacgccta gttatgaccc aaacctgttt
    30241 gttgacggta tctccagcaa agattattcc gccttgttga acgccccgaa tacaccgctg
    30301 gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta cagttaaacc ctatgtggcg
    30361 gtttcggcat tgagcgccgg ggtgatcacg cgcaatacga cgctgtttga cccaggctgg
    30421 tggcaactgc caggttcgga aaaacgttat cgtgactgga aaaaatgggg ccacgggcgt
    30481 ctgaatgtca caagatcgct ggaagaatct gcggatacct tcttctatca ggtggcctac
    30541 gatatgggga tcgatcgcct ctccgaatgg atgggtaaat tcggttatgg tcattacacc
    30601 ggtatcgacc tggcggaaga acgttccggc aacatgccta cccgcgaatg gaaacagaaa
    30661 cgctttaaaa aaccgtggta tcagggtgac accattccgg ttggtatcgg tcagggttac
    30721 tggacagcga ccccaatcca gatgagtaag gcactgatga tcctgattaa tgacggtatc
    30781 gtgaaggttc ctcatttgct gatgagcacc gccgaagacg gcaaacaggt gccatgggta
    30841 cagccgcatg aaccgcccgt cggcgatatt cattccggtt actgggagct ggcgaaagac
    30901 ggtatgtacg gtgttgctaa ccgccctaac ggtacggcgc ataaatactt tgccagcgca
    30961 ccgtacaaaa ttgcggcgaa atccggtacc gctcaggtct tcggtctgaa agcgaacgaa
    31021 acctataatg cgcacaaaat tgccgagcgt ttacgtgacc acaaactgat gaccgccttt
    31081 gcgccataca acaatccgca agtggctgtc gccatgattc tggagaacgg tggtgcgggt
    31141 ccggcggttg gtacactgat gcgccagatc ctcgaccaca ttatgctggg tgataacaac
    31201 accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg aggaccatta atcatgacgg
    31261 ataatccgaa taaaaaaaca ttctgggata aagtccatct cgatcccaca atgctgctga
    31321 tcttactggc attgctggtt tacagcgccc tggttatctg gagcgccagc ggtcaggata
    31381 ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg tctagtcatc atggtggtga
    31441 tggcgcaaat tcctccacgc gtttatgaag gctgggcccc ctatctctat atcatctgta
    31501 ttattttgct ggtggcggta gatgctttcg gtgccatctc taaaggtgct caacgctggc
    31561 tggacctcgg tattgttcgt tttcagccgt cggaaattgc caaaatagcc gtaccactga
    31621 tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc gttgaagaac actggcatcg
    31681 cgctggtgct gatatttatg cccacgctgc tggtggctgc acagcctgac ctgggaacat
    31741 caatcctcgt tgcgctttcc ggtctgtttg tactgttcct ctctggcctt agctggcgtc
    31801 tgattggcgt cgcagtagtg ctggtggcgg cgttcattcc gattctgtgg ttcttcctga
    31861 tgcatgatta ccagcgccag cgcgtaatga tgctcctaga cccggaatca gacccactcg
    31921 gcgcgggcta tcacattatt cagtctaaaa ttgctattgg ctccggcgga ttacgcggca
    31981 aaggctggct gcacggcact cagtcacagc ttgaatttct ccccgaacgc cataccgact
    32041 ttatcttcgc ggtactggcg gaagagctgg gattagtggg cattctgatt ctgctcgctc
    32101 tctacattct gctgatcatg cgcgggctgt ggatagccgc cagagcgcaa accacctttg
    32161 gtcgcgtcat ggctggtggc ttaatgctga tattattcgt ttatgtcttc gtaaatattg
    32221 gtatggtaag cggtattctg ccggttgtag gggttccgct cccactggtc agttatggag
    32281 gatcggcgct aattgtgctg atggctgggt tcgggattgt aatgtcaatc cacacccaca
    32341 ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc gtaagcagtg gctcgggatc
    32401 tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg atggtcagca acagacggta
    32461 agtgtaccgc agcctgcggt atgtaacggc cccatagttg aaattagcgg ggcggacccg
    32521 cgtttcgaac cactgaacgc gacggcaaat caggattacc agcgcgacgg taaaagctac
    32581 aaaatcgtgc aggatccgtc tcgatttagc caggcgggac tggcggcaat ctatgatgcc
    32641 gaacccggca gtaacctgac ggcctctggc gaagctttcg atccgacaca gctgacggcg
    32701 gcccatccaa cgcttccgat ccccagctac gccagaatca ctaacctggc taacgggcga
    32761 atgatcgtgg tgcgcattaa tgatcgcggc ccttacggca acgacagcgt tatttcactt
    32821 tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca ccaaagttcg tatcgatccg
    32881 attattgtcg cccaggatgg ttcgctttct ggtcctggta tggcttgtac cacagtcgcc
    32941 aaacagactt acgccctgcc agcacctccc gatttaagcg gtggcgcggg aacaagttca
    33001 gtgtctggcc cgcagggtga cattcttccg gtcagtaatt cgacgctaaa aagcgaagat
    33061 ccgaccggcg cgccggtaac cagtagcggt ttcctcggcg ctccaacgac cttagcgcct
    33121 ggtgtactgg aaggcagcga accgacgcct gcgccacagc ccgttgttac agctccgtcg
    33181 acaacgcctg caacctcgcc tgcaatggtg acaccgcaag ccgcctcgca aagcgccagc
    33241 ggcaacttta tggtgcaagt cggggccgta agcgatcagg ctcgtgcgca acagtaccaa
    33301 cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa ctcaaaatgg cgcggtctgg
    33361 cggatccagc ttggcccatt cgccagcaaa gccgaagcca gtaccttgca gcaacgtttg
    33421 caaaccgaag cccaattaca gtcatttatt actaccgcgc agtagcgtaa agcaggcatc
    33481 tgatgtgtca atttctgtaa atgacgttaa caaagtcacg cggaaagtca gatgcctgcc
    33541 ggtagtgcat ttgctatagt agggcacttt tttaattcca tcacggatgt cgtagttcag
    33601 accatgaata ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt
    33661 tgcacagcct ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg
    33721 ggtgtaccgc agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg
    33781 ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc
    33841 agttacgtta tcggccaggc aatgaaagcc ggtaaattta aagaaactga tttagtcact
    33901 atcggcaacg acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc
    33961 ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg
    34021 ggtaacgatg cttgtgtcgc catggccgat tttgccgctg gtagccagga cgcttttgtt
    34081 ggcttgatga acagctacgt taacgcactg ggcctgaaaa atacccactt ccagacggta
    34141 catggtctgg atgctgatgg tcagtacagc tccgcgcgag atatggcgct gatcggccag
    34201 gcattgatcc gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt
    34261 aacggtattc gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac
    34321 ggcatcaaaa ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa
    34381 ggccagatgc gcttgatttc tgcggtgatg ggcggacgta cttttaaagg ccgtgaagcc
    34441 gaaagtaaaa aactgctaac ctggggcttc cgtttcttcg aaaccgttaa cccactgaaa
    34501 gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta
    34561 ggggttgata aagacgtgta cctgaccatt ccacgtggtc gcatgaaaga tctgaaagcc
    34621 agctatgtgc tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga
    34681 actatcaact tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa
    34741 gaaatcccgg aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat
    34801 cactggtttg gttaaaaatt gaacacttga aagtgtaatt tccgtcccca tatactaagc
    34861 atcagtaaaa aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc
    34921 tgacatgaaa accaaactta acgaactgct tgaattccct actcctttta cttacaaagt
    34981 tatggggcag gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc
    35041 gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc
    35101 tatcactatc aacgccactc atatcgagca ggttgaaaca ctgtatgaag aactaggcaa
    35161 aatcgatatt gtccgcatgg ttctgtaact cgcttctccg ttacccggcc cctggtcggg
    35221 taactcccct ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag
    35281 atgcagtttt gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc
    35341 caatctccca ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa
    35401 tctggctggt cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca
    35461 ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc
    35521 acgggccggg gcaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg
    35581 tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta
    35641 tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct
    35701 cactgggttt acgtattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata
    35761 tggatctttc accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta
    35821 aaatatcaca atggaaaccc gaagcgacga ctaataatat tgcaccacgt ttactggaaa
    35881 atattttagc gctactaaac aatcctgact tcgaatatat taccgcttaa ttgcatacct
    35941 caatggccca atttacactg ggtcattact cataatacct gctaatgctt tacatctcct
    36001 tcgcgtttgt atactttctt tatcacttgc agaaatgcgg tgctacgcct tgctccgtaa
    36061 ctcaccccat tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc
    36121 caattagaca taagattatt gtaatttttt taaatattat ttttccatga ataaaattgg
    36181 agtaagtgcg tggatagtaa taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa
    36241 ggtaaaccac aaatatttac aaccttacga aatattgatc ttaaccttct gactattttt
    36301 gaagctgtat atgtacataa agggatcgtt aatgcagcga aagtgcttaa tctgaccccc
    36361 tcggcaatca gtcagtctat tcagaaactg cgcgttatat tccctgaccc attgtttatt
    36421 cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc atctacatga gtatatcagt
    36481 cagggccttg agtccattct tggcgcgctg gatatcgaag gaagctatga taagcaacga
    36541 acgataacta ttgcgaccac tccctcggtc ggagccctgg tccttcctgt catctatcgg
    36601 gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa
    36661 aaccaactca gtcagtttca aaccgatctc atcatcgata acatgttttg caccaatcgt
    36721 acggtgcaac atcatgttct gttcaccgac aatatggtgt taatttgccg cgagggaaat
    36781 ccactactct ctttagaaga tgacagagag actatcgaca acgctgcgca tgtactcctg
    36841 ttaccggaag ggcaaaattt cagcggtctg cggcagagag ttcaagagat gtttccggac
    36901 cggcaaatca atttcaccag ctacaacatt ttgacaatcg ctgcactggt tgccaacagt
    36961 gacatgttag cgattattcc cagccgtttt tataacctgt ttagtcgttg ctggccgctg
    37021 gaaaaactgc cttttccgtc cttaaatgag gagcaaatag atttctctat ccactacaac
    37081 aaattcagcc tgcgtgatcc catcctgcac ggggtaatag atgttatccg taatgcattt
    37141 tgaaaaagta ttgttacagc cagatcacag cgcagaacga taaaatggca caatccaaca
    37201 acaattttac attttcgcga ccgctttggc tgctttcagg tccgtttcaa tgatatactg
    37261 ccagtcgtta attcaaaaat agttgataat tacaacaatc tattgaattg aaacgctttc
    37321 cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg
    37381 tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tggcaacaga
    37441 gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac
    37501 acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga
    37561 agcctcctgc cctaacctgg cggaatgctt caaccacggc acagcaacgt ttatgatcct
    37621 cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc
    37681 tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg cgctgcgtta
    37741 tgtggttatc acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc
    37801 ggattgcatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc
    37861 ggatttccgt ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt
    37921 gttcaaccat aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga
    37981 ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac
    38041 caagtctggt ctgatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg
    38101 cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg caatatttgc agccaagccg
    38161 ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga
    38221 agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgct cttcttacca
    38281 cgccgatttg caggcgaaag ggatggaagt taagtaatcc tgtaataatt acttacatct
    38341 gtctgataaa aaacccgctt tttg
//
LOCUS       DAJTXQ010000027        37741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000027 DAJTXQ010000000
VERSION     DAJTXQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.027"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..827
                     /locus_tag="OO515_001297"
     CDS             <1..827
                     /locus_tag="OO515_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575268.1"
                     /translation="LTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLNFGIMCL
                     HMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRKMKQVFV
                     FCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAGYKGTAM
                     GVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPPYVSSLR
                     IEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQAIRQA"
     gene            complement(955..1446)
                     /gene="yajQ"
                     /locus_tag="OO515_001298"
     CDS             complement(955..1446)
                     /gene="yajQ"
                     /locus_tag="OO515_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6575269.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1614..2525
                     /gene="panE"
                     /locus_tag="OO515_001299"
     CDS             1614..2525
                     /gene="panE"
                     /locus_tag="OO515_001299"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6575270.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2488..3078
                     /gene="yajL"
                     /locus_tag="OO515_001300"
     CDS             2488..3078
                     /gene="yajL"
                     /locus_tag="OO515_001300"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6575271.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3132..4580)
                     /gene="thiI"
                     /locus_tag="OO515_001301"
     CDS             complement(3132..4580)
                     /gene="thiI"
                     /locus_tag="OO515_001301"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6575272.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4786..5028
                     /gene="xseB"
                     /locus_tag="OO515_001302"
     CDS             4786..5028
                     /gene="xseB"
                     /locus_tag="OO515_001302"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6575273.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5028..5927
                     /gene="ispA"
                     /locus_tag="OO515_001303"
     CDS             5028..5927
                     /gene="ispA"
                     /locus_tag="OO515_001303"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6575274.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5952..7814
                     /gene="dxs"
                     /locus_tag="OO515_001304"
     CDS             5952..7814
                     /gene="dxs"
                     /locus_tag="OO515_001304"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6575275.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7869..8843
                     /gene="yajO"
                     /locus_tag="OO515_001305"
     CDS             7869..8843
                     /gene="yajO"
                     /locus_tag="OO515_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6575276.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(8897..9415)
                     /gene="pgpA"
                     /locus_tag="OO515_001306"
     CDS             complement(8897..9415)
                     /gene="pgpA"
                     /locus_tag="OO515_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6575277.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9393..10370)
                     /gene="thiL"
                     /locus_tag="OO515_001307"
     CDS             complement(9393..10370)
                     /gene="thiL"
                     /locus_tag="OO515_001307"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6575278.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(10448..10867)
                     /gene="nusB"
                     /locus_tag="OO515_001308"
     CDS             complement(10448..10867)
                     /gene="nusB"
                     /locus_tag="OO515_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6575279.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10887..11357)
                     /gene="ribE"
                     /locus_tag="OO515_001309"
     CDS             complement(10887..11357)
                     /gene="ribE"
                     /locus_tag="OO515_001309"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6575280.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11446..12549)
                     /gene="ribD"
                     /locus_tag="OO515_001310"
     CDS             complement(11446..12549)
                     /gene="ribD"
                     /locus_tag="OO515_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6575281.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12553..13002)
                     /gene="nrdR"
                     /locus_tag="OO515_001311"
     CDS             complement(12553..13002)
                     /gene="nrdR"
                     /locus_tag="OO515_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6575282.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13153..13692
                     /locus_tag="OO515_001312"
     CDS             13153..13692
                     /locus_tag="OO515_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6575283.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13991..14875
                     /gene="tsx"
                     /locus_tag="OO515_001313"
     CDS             13991..14875
                     /gene="tsx"
                     /locus_tag="OO515_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6575284.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15052..15399)
                     /locus_tag="OO515_001314"
     CDS             complement(15052..15399)
                     /locus_tag="OO515_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6575285.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15528..16499)
                     /gene="secF"
                     /locus_tag="OO515_001315"
     CDS             complement(15528..16499)
                     /gene="secF"
                     /locus_tag="OO515_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6575286.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16510..18357)
                     /gene="secD"
                     /locus_tag="OO515_001316"
     CDS             complement(16510..18357)
                     /gene="secD"
                     /locus_tag="OO515_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6575287.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18385..18717)
                     /gene="yajC"
                     /locus_tag="OO515_001317"
     CDS             complement(18385..18717)
                     /gene="yajC"
                     /locus_tag="OO515_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6575288.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18740..19867)
                     /gene="tgt"
                     /locus_tag="OO515_001318"
     CDS             complement(18740..19867)
                     /gene="tgt"
                     /locus_tag="OO515_001318"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6575289.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19923..20993)
                     /gene="queA"
                     /locus_tag="OO515_001319"
     CDS             complement(19923..20993)
                     /gene="queA"
                     /locus_tag="OO515_001319"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6575290.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21086..21667
                     /gene="acpH"
                     /locus_tag="OO515_001320"
     CDS             21086..21667
                     /gene="acpH"
                     /locus_tag="OO515_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6575291.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(21672..23489)
                     /gene="malZ"
                     /locus_tag="OO515_001321"
     CDS             complement(21672..23489)
                     /gene="malZ"
                     /locus_tag="OO515_001321"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6575292.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(23645..25018)
                     /gene="proY"
                     /locus_tag="OO515_001322"
     CDS             complement(23645..25018)
                     /gene="proY"
                     /locus_tag="OO515_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6575293.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25094..26413)
                     /gene="brnQ"
                     /locus_tag="OO515_001323"
     CDS             complement(25094..26413)
                     /gene="brnQ"
                     /locus_tag="OO515_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6575294.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26820..28115)
                     /gene="phoR"
                     /locus_tag="OO515_001324"
     CDS             complement(26820..28115)
                     /gene="phoR"
                     /locus_tag="OO515_001324"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6575295.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28173..28862)
                     /gene="phoB"
                     /locus_tag="OO515_001325"
     CDS             complement(28173..28862)
                     /gene="phoB"
                     /locus_tag="OO515_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6575296.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29052..30254
                     /gene="sbcD"
                     /locus_tag="OO515_001326"
     CDS             29052..30254
                     /gene="sbcD"
                     /locus_tag="OO515_001326"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6575297.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30251..33394
                     /gene="sbcC"
                     /locus_tag="OO515_001327"
     CDS             30251..33394
                     /gene="sbcC"
                     /locus_tag="OO515_001327"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6575298.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33520..34704
                     /gene="araJ"
                     /locus_tag="OO515_001328"
     CDS             33520..34704
                     /gene="araJ"
                     /locus_tag="OO515_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6575299.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(34847..35755)
                     /gene="mak"
                     /locus_tag="OO515_001329"
     CDS             complement(34847..35755)
                     /gene="mak"
                     /locus_tag="OO515_001329"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6575300.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            35880..36791
                     /gene="rdgC"
                     /locus_tag="OO515_001330"
     CDS             35880..36791
                     /gene="rdgC"
                     /locus_tag="OO515_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6575301.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36869..37324)
                     /locus_tag="OO515_001331"
     CDS             complement(36869..37324)
                     /locus_tag="OO515_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575302.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37321..37656)
                     /locus_tag="OO515_001332"
                     /pseudo
     CDS             complement(37321..37656)
                     /locus_tag="OO515_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9366 a        10161 c         9557 g         8657 t
ORIGIN      
        1 cgttgaccat ttgggttgtg cccaacagta gcactcacgt acttaatcgt gagtccggaa
       61 tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg gctgctgaaa ctcaactttg
      121 gcattatgtg tctgcacatg cttctgatgt ctacgtttgt tgccctgccc ggacagctag
      181 ctgatgcggg gttcccggcg gctgaacact ggaaggtcta tctggcgacg atgttaatcg
      241 cctttggctc gatcgtgcct ttcattatct acgctgaagt taagcgcaaa atgaagcaag
      301 tctttgtctt ctgcgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcacaaa
      361 cgcagttctg gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg
      421 aagcccttct gccctcactt atcagtaaag agtcgccagc aggttacaaa ggtacggcga
      481 tgggtgtcta ctccaccagc cagtttcttg gcgtggcgat tggcggttcg ctgggcggct
      541 ggattgacgg catgtttgac ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag
      601 tgtggctggc agtcgccagt accatgaaag aaccgccgta tgtcagcagt ttgcgcattg
      661 aaatcccggc ggacattgcc gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag
      721 gcgtcaaaga agtgttgatt gcagaagaag agcattcagc ttatgtgaaa atcgacagca
      781 aagtgacaaa tcgctttgag gttgaacagg caattcgtca ggcataaaaa acgcggcgaa
      841 ttgcctgctg cgctacgctt accaggccta caaagttctt gagttacaaa ccgttgtagg
      901 ccgaatcagg cgttcatgcc gcatctgaca tgaacaaaac gcacattgtc gcgattaatc
      961 gcggaagttt ttgaactgga acggctggcc gagatcgcca ccacgtacca tcgccatgac
     1021 agcctgcaaa tcatcacgag atttgccagt cacacggatc tcatcgccct gaatttgcgc
     1081 ctgcactttc agtttgctgt ctttaatcat cttgacgatt ttcttctggg tcgcgctctc
     1141 aatgccctgt ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattttc
     1201 cggtacatcc agtgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc
     1261 cagcaactga ttgacctgga aatcggactc gctcaacact ttgatggttt tgctggcgtc
     1321 gttcagctca aatgaggctt caacattacg gaagtcaaaa cgggactcca cttcgcggct
     1381 cgcgttatcg accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga
     1441 tggcatcttt tcctctccct tcatttttga tgcgaagcat aatacccgca aagttaaata
     1501 accgacagtc ctgcaccagg acgtatccct taattgtgca tagtcacaac agcatgcaca
     1561 attaagggat agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa
     1621 ttaccgtatt gggatgcggt gccttagggc aattatggct tacagcactt tgtaaacagg
     1681 gtcacgaagt tcagggctgg ctgcgcgtac cgcaacctta ttgtagtgtg aatctggttg
     1741 agactgatgg ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttcttgcca
     1801 ccagcgatct gctcctggtg acgctgaaag catggcaggt ttccgatgcc gtcaaaagcc
     1861 tcgcgtccac actgcctgta accacgccaa tactgttaat tcacaacggc atgggcacca
     1921 tcgaagagtt gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc
     1981 gccgcgacgg caatgtcatt attcatgtgg caaacggaat cacgcatatt ggtccggcac
     2041 ggcaacagga cggcgattac agctatctgg cggatatttt gcaaaccgtg ttgcctgacg
     2101 ttgcctggca taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga
     2161 ttaatccact gaccgccatc tggaattgcc cgaatggtga attacgtcat catccgcaag
     2221 aaattatgca gatatgcgaa gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt
     2281 cagcagaaga tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct
     2341 cgtcgatgtt gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt
     2401 ttctcttacg ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg
     2461 aaatggtaaa aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct
     2521 ggtagtgaag agactgaagc cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa
     2581 gtcaccactg ccagcgtcgc cagcgatggc aacctggcca ttacctgctc gcgcggcgtg
     2641 aagctgctgg cggatgcgcc gctggtcgag gtggctgatg gcgaatatga cgtgatcgta
     2701 ctgcctggtg gcattaaagg cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc
     2761 gttaaacagt tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc
     2821 gtgctggtac cgcacgatat cttcccgatt ggcaatatga ccggcttccc gacgctgaaa
     2881 gacaaaattc ccgccgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa
     2941 ttgctgacca gccaggggcc gggtacggct attgattttg gtctgaaaat tatcgacctg
     3001 ctggttgggc gcgagaaaac ccatgaagtg gcatcacaac tggtgatggc ggcagggatt
     3061 tataattatt acgagtagtg tcggatgcgg caaacgtcgc atctgaccag atgcgacgta
     3121 aaaaccacaa attatgggcg atacaccttc acattgttaa agccctgctc gcgcagatag
     3181 agcgcctgca gacggctcat caccccgcgc tcacaccaca gtagccaggt tctgttctgg
     3241 tcgagatcgc caaatttggt gctcagttta tagaacggca gagaaaccac gtcgatacct
     3301 tcaaccttca gcggcttatc ttcctgctca tcgacagaac ggatatcgag gatcacgtcg
     3361 ttcgggccaa agccattgac ggtttccact tccaccactt cctgctcggt ctgctgggcg
     3421 atttcgcgga tatcaacgtt attcgcttcc tcaaccactt tatcgagaat actgaagtcg
     3481 aacttctctt cttccgcttc aatcttcgat ttaaccgctt tcaccgtcgg gcttttggag
     3541 atcacgccac aatattctgg catcgtgcga gcaaagtctt cagtgccaat ctggcgggcc
     3601 aggttgatga tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtatcagag
     3661 acgttatcaa tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg
     3721 ccggtgacca gcgcctgtac gccgtaacgt tcagccactt tagacgcggc acgcaccatc
     3781 atacgtttga ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgacg
     3841 accggttcga aattaatggc gacaaaacgc acgcggtggg agctgccaaa gcggttccac
     3901 agataatgcg ccacctgacg cacgccaatt tcatgcgctg cgccgccgag gttaaagaag
     3961 cagtaatgca cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa
     4021 ccaccggaaa tgagcgacag cacatcttcc tgggtgccaa tcgggaaacc gccaatacct
     4081 tcgtagcggc ctttaatcag cagaagacga tcgtcttcca cttccagatg gacagttaca
     4141 tccgggttag tcagcttcac gcgcgcggat tcaatatgct gatttaaacc gccgccgacg
     4201 taacgttcca catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag
     4261 aaggttttgc cttccagctg atcgcgatat tgaaccaacg ctttctcgaa aatatcgtgc
     4321 atgtcggtaa acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc
     4381 agggcgtcgc gaatagccag acgctgtttt tcatcttttg cgcgaacttc gatgttatcc
     4441 cagtggcgga cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg
     4501 gtaaggattt ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac
     4561 aatttaatga taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat
     4621 agggaaaaat acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa
     4681 catcgctttg ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc
     4741 cgttaccatg acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat
     4801 gaggcgcccg ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg
     4861 gaaagtggcg acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg
     4921 gcacgtcagg ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct
     4981 gacaatgaag acacctctct accccctttt acaccggaca atgagtaatg gactttccgc
     5041 agcaacttga agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac
     5101 tgccctttca gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta
     5161 agcgcctgcg accttttctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca
     5221 cgctggacgc acccgctgcc gccgttgagt gtatccacgc ttactcatta attcatgatg
     5281 atttaccggc gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt
     5341 ttggcgaagc aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattt
     5401 taagcgatgc cgatatgccg gaagtgtcgg accgcgacag aatttcgatg atttcagaac
     5461 tggcaagcgc cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagatgcgg
     5521 aaggcaaaca cgtacctctg gacgcactcg agcgtattca tcgtcataaa accggcgcat
     5581 tgattcgcgc cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc
     5641 tgccagtact cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca
     5701 tcctggatgt ggtgggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac
     5761 ttggtaaaag tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg
     5821 atctgatcga cgatgcccgc cagtcgctga aacaactggc tgaacaatca ctcgatacct
     5881 cggcactgga agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta
     5941 ataggcccct gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca
     6001 cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc
     6061 gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg
     6121 tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg
     6181 atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca
     6241 ccatccgtca gaaaggcggt ctgcatccgt tcccgtggcg cggcgaaagc gaatatgacg
     6301 tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttactg
     6361 ccgagaaaga aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg
     6421 ctggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtgg
     6481 tcctcaacga caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaat aaccatctgg
     6541 cacagctgct ttccggtaag ctttactctt cgctgcgcga aggcgggaaa aaagttttct
     6601 ctggcgttcc gccaattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg
     6661 tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg gttgacggtc
     6721 acgatgtgct ggggcttatc accacgctga agaacatgcg cgacctgaaa ggcccgcagt
     6781 tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca
     6841 ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg
     6901 gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca
     6961 acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac
     7021 gtaaattccc ggatcgttac ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg
     7081 ccgccggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc
     7141 aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagctcccg gtcctgttcg
     7201 ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggc gcttttgacc
     7261 tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat
     7321 gtcgccagat gctctatacc ggctatcact ataacgacgg cccgtccgcg gtgcgctacc
     7381 cgcgcggtaa cgcggttggc gtggaactga cgccgctgga aaaactgcca attggcaaag
     7441 gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag
     7501 acgcggcgaa agtcgctgaa tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac
     7561 cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag
     7621 aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgcta atggcccatc
     7681 gtaaaccagt acccgtgctg aacattggcc tgcctgactt ctttattcca caaggaactc
     7741 aggaagaaat gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg
     7801 cctggctggc ataatcccta ctccactcct gctatgctta agaaattatt catggcagga
     7861 gtggcagcat gcaatacaac cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc
     7921 tcggctgtat gacctttggc gagccagatc gtggtaatca cgcatggaca ctaccggaag
     7981 agagtagccg tcccatcatt aaacgcgcgc tggaaggcgg cataaatttc tttgataccg
     8041 ccaatagcta ttccgatggc agcagcgaag agatcgttgg tcgcgcactg cgggatttcg
     8101 cccgtcgtga agacgtggtc gttgcgacga aagtgttcca tcgcgttggt gatttaccgg
     8161 aaggattatc ccgtgcacaa attttgcgct ctatcaacga cagcctgcgc cgtctcggca
     8221 tggattatgt cgatatcctg caaattcatc gctgggatta caacacgccg atcgaagaga
     8281 cgctggaagc cctgaacgac gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat
     8341 ccatgcacgc ttcgcagttt gctcaggccc tgaagctaca aaaacagcac ggctgggcgc
     8401 agtttatcag tatgcaggat cactacaatc tgatttatcg cgaagaagag cgcgagatgc
     8461 tgcctctgtg ttatcaggag ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc
     8521 gactgacgcg tccgtgggga gaaactaccg cacgactggt gtctgatgag gtggggaaaa
     8581 atctctataa agaaagcgat gaaaatgacg cgcagatcgc agagcggtta acgggcgtca
     8641 gtgaagaact cggtgcaaca cgagcacaag ttgcgctggc ctggttattg agtaaaccgg
     8701 gcattgccgc accgattatc ggtacatcgc gggaagaaca gcttgatgag ctgctgaacg
     8761 cggtggatat cactttgaag ccagaacaga ttgccgaact ggaaacgccg tataaaccgc
     8821 atgcggtcgt aggatttaaa taagagtcca ggcctgatga gacgtgacaa gcgtcacatc
     8881 aggcatcggt gcacaactac gacagaatac ccagcggcca gtgatgaccg ataaagtaca
     8941 ggatgcctgc ggaaatcacc ccggcgacaa tatcgtcgat catgatcccc atgccgccat
     9001 gtacattgcg atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa
     9061 tcacaaaccc ggcggcaacc cactgccagt cattggtcgg cagcgccatg agcgtgatcc
     9121 acataccaat aaattcgtcc cagacaatgc tgccgtgatc gtgcacgccc atatctttcg
     9181 ccgtttgatg acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga
     9241 gctgccaggg taaaaaggtc atcagatacc agaacggaat cgctgccagc gatcccatcg
     9301 tgccaggaac gattgggctt aatccactcc cgaatccgac agcaagcaga tgccacggat
     9361 tactcatctt caggcgactt ttcgcgacat ctttatggcg tggcaaaatg gtcatatcct
     9421 ttccagtcaa acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg
     9481 gtcatttgcc cgatacaggt aaacggtacg ccaaggtgtc cgagagcaac atccagcgcg
     9541 ccacggttca gttccggcac cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc
     9601 cagcgcagcg cctgttccgg ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat
     9661 gccaggtcaa tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc
     9721 agaccgtcag agagatcgat agctgaattt gccagatcgc gcagtgcctg cccctgtaaa
     9781 atacgcggcg atggacggag atgacgtttg atcaagtagt cagcatcttt agcatcggca
     9841 acctgcaaac ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc
     9901 acatagatcc agtcacccgg tttcgcccca gagcgcgtta aggctcgtcc catcggaaca
     9961 aagccgtgga tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt
    10021 tgcatatcgt aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct
    10081 tcgtctacgt ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct
    10141 gccagatcgc ttaggttcac cgccagtgct ttataagcca gatcagcagg atcgatatca
    10201 gggaggaaat ggttacccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg
    10261 gggatattga gaagtgcgca atcgtcgccg atgcccagtt cgacatcaag acgagaactt
    10321 cttacacggt caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc
    10381 tcagcaggta aaaaagaaaa ggccgacgat catggaacgg tcttccgtga atctaccggc
    10441 ctggatatca ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga
    10501 cgaacttatg gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg
    10561 ccactttgta tggcacatcg ctacgtttag acagttcgta cagcgcaatg cgcagtactg
    10621 ctttttctac ctgtcccagt tcttccagca ggcgggacag gtatggcttc atcagtccgt
    10681 cgaggtatgc ggtattagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa
    10741 cgtcttttac atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg
    10801 acaactgcca ggagtagagc gcctggacgg cacactcacg agcgcggcga cgagcagcag
    10861 gtttcacgga tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt
    10921 tcaagcgcgg tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg
    10981 atcgcttgtt caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc
    11041 tgggcaacat gcgccaggcc gttgcttgca ccaccagcga catattcaaa gtgggcagtg
    11101 ccaccacgaa taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt
    11161 gcacccgccg ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca
    11221 tcttttacct gaccgatacg tttcagtgcg tcaattgcac cttccagcag gctgtcattg
    11281 ataaagttgt tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta
    11341 gcttcaataa tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat
    11401 tttagcataa tatttcgtgc gctgcttccc tttcgagccg ggagatcatg cacccactaa
    11461 atgcaggcaa acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc
    11521 ggctaatttc tcaagccctg gcagcgtgca taatccgcgg gcgtcgctgc ctaatagttt
    11581 aggtgcgata tagacaatca gctcatcgac taaacccgcc tgcagcaatg cgccagcgag
    11641 cgttggcccc gcttccaccc agatgctgtt aatttgctgt ttacccagtt gcatcatcag
    11701 tacaaccaga tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg
    11761 ccactcacta gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat
    11821 gcgatgttcc ggcgtcacgc gattttggct atcaatcaca atacgtactg gctggcggag
    11881 attttgttgc gggtagagcg cctgagtttg ttcatccagt tcagaccaac gcactgttaa
    11941 ggctggatca tccgccagca ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg
    12001 ctgtcgttgt acgtcgcgcc gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc
    12061 catcgccgtg cggccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt
    12121 gcgcatccgc ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcagcccgtg
    12181 gctgacgtca atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt
    12241 aggatcctgc atcgctgcga ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca
    12301 cggtggcgta cggccatgat ggctacaggg ttcgagtgtc acatacgccg ttgcgccttt
    12361 ggctttttca cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg
    12421 gtggtaacct tcaccgacaa tttcgccatc tttgacaatg acgcccccga cattcgggtt
    12481 ggggtgggtg gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc
    12541 gtcctgcacg gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc
    12601 gaaactgcgg tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg
    12661 ctccatcacc agattgccaa tcatcttgct cggtacttcg cgctcaccgg tggcgcgcag
    12721 ctgcgattta atatgattga ttgccatttc aacgtcatcg gaactcaccg gacgtttttc
    12781 cagcgcccgc agcattccgc tacgcaattt ctcttcatta aacggttcac gcacgtcgtt
    12841 gctttttaca acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt
    12901 acacaccaga cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc
    12961 aattacctta gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt
    13021 ggttaacgga aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga
    13081 gataaaggat cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga
    13141 aagggacgac ctatgaatac taacgttttt cgactgctcc tgctgggaag cctgttcagc
    13201 cttagcgcct gtgtgcagca aagtgaagtg cgacagatga aacacagcgt cagcacgctg
    13261 aaccaggaga tgacgcaact caatcaagaa acagtcaaaa tcactcagca aaacaggctg
    13321 aatgcaaaat ccagcagtgg ggtttacctt ctgcctggag cgaaaacacc ggcaagactg
    13381 gaaagccaga tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tacagatggc
    13441 accaccctta cactacgtat tcagggtgag tcgaatgatc ccttgcccgc attcagcgga
    13501 actgttgaat atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtgcag
    13561 aatcaattga ttaatgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta
    13621 caattaaagg ggatttctgt ggatcagtta ggctttgtac gtatccacga cattcaacct
    13681 gttatgcagt aaacgtattt cgggacgatt ttgtgcgtcc cgcaacatct ttccccgtca
    13741 ttttgttact ctgcttacat cacctggatt gatagtaaaa gtttgcaaca agggcgaaag
    13801 tcagtacaat ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata
    13861 gaactgtgaa acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat
    13921 acgaaatatt tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag
    13981 tggcatacat atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc
    14041 ttttactgtc aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca
    14101 gagcgttaac gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac
    14161 ctaccttgag tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga
    14221 tgcgccggta ttcttcggcg gtaactccga tgctaaaggt atctggaacc acggttctcc
    14281 gctgtttatg gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag
    14341 cttcggtccg ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa
    14401 taaagatggt cgccagagca cctggtacat gggtctgggt accgacatcg atactggtct
    14461 gccgatgagc ctgtccatga acgtctatgc gaaataccag tggcagaact atgacgcagc
    14521 gaacgaaaac gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga
    14581 tctgtggggc ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt
    14641 aggggatgac agcggtaacg caatcaacgg tattaagacc cgtactaata actctatcgc
    14701 ttccagccat attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta
    14761 ctggcacgac ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt
    14821 caacgttcgc tctaccggct ggggtggtta cctggtagta ggttacaact tctgattatg
    14881 aaaatgccgg gatttattcc cggcatttct gattgttaac aacgtgtgct ttgttcatgc
    14941 cggatgcggc gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa tatattgctg
    15001 aaataacgta ggcctgataa gcgtagcgca tcaggcaatt ttacgtttta atcacttctt
    15061 cttgttcatc atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt
    15121 ctgcgcatct tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga
    15181 atgctcatga tcgtggcaat agagacaaag caattcccag ttactgccat cttccgggtt
    15241 attggtatgg tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttgg aataaacaaa
    15301 ctcgcgggaa cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc
    15361 gctttctaac cgcgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat
    15421 gtagggttac gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa
    15481 aaatcccgat cttctgaccg ggattttcaa catcaacggg aacttgatta cggcagaatt
    15541 gacggctgat ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata
    15601 cccagtttca gagccaacgc agatgctacg tagatggaag atgcagtgcc gatggaaaca
    15661 ccgataagca tggtcagcga gaagccttcc agtaccggac caccgaagag atacagcatc
    15721 aggataacca tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag
    15781 gacacgttaa agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaatt
    15841 cggtccgata ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc
    15901 acaatggtca ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg
    15961 tcgtgcgcca gcgcaataac cacccctgcc gccagtcgcc actcaaagcg gaaacctacg
    16021 tacacgagga tagacagcag cgctgccatc aacgccatcg caccggtttg cgcaaggtct
    16081 gcccccacgc tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat
    16141 tcgttaatca ccttcagaac ctggctgccc agcacctgac cgccggtttc gccttcagca
    16201 ggcggcatac ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca
    16261 aaaccggctt tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta
    16321 atttcgataa ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg cacgcccata
    16381 ataacgatag cagcgattaa cagcagacca gagatgccga aagcccagta gtcccagcgc
    16441 ataaagtcat agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca
    16501 tcgcactcct cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat
    16561 ggcacgcgta ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat
    16621 cgcgaaccct ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt
    16681 ggtgatgttc gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg
    16741 ttgaacagta cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc
    16801 atcgaccgct accgcaaggg ttaagacgat acccgcgata cctggcatac tcagtgttgc
    16861 gcctggcaac agcgacataa taccgacgat caagatcaag ttggcaatca gagcactggt
    16921 cgcaatcaga ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc
    16981 ggcaaggcaa gcctccagcc cctgctcaat gttctgcata cccagggtcg ggccaattgt
    17041 acgttcttca acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag
    17101 ctgacgggct tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga
    17161 ctggatgttg gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc
    17221 atctttctta ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt
    17281 gtccttggtg aagttagaca taatgttgcc accagcgcta tcgagcgaga tgttaacctg
    17341 cggctggttg tattcgtcct ggctggaagt ggagtcggtg atatggtcac cggtcagaat
    17401 tacgcgtttg tacagcacaa ccggctgacc ttcgcgggtc tgtttcactt cagagtcgcc
    17461 cggtacgcga ccggatgccg cagcggcctg atcaacattg gtgtttacca gacggaattc
    17521 cagcgttgcc gtcgcgccca gaatctcttt cgcacgcgca gtgtcctgaa taccaggcag
    17581 ttcaacaacg atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg
    17641 gtttacacgg ttacgcagga tattaatgtt ctgctgcacc gcatattcac gcgcttcact
    17701 cagacgagca tcgctcatta ccgcacgcag ctggttgctg ccctggctgc taatcaccag
    17761 gtccggatgg cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa
    17821 agtgatgctc aggccgtagt tgttttcttt acgaacagtg gtatacggga tgcccttttc
    17881 gcgcaggtca ctgcgtaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc
    17941 catatccact tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg
    18001 ctcagcatga atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac
    18061 gtatttgtca cccataacgc ccattaatgc ttcacgagcg cgcaactggg tgtcagtgga
    18121 gtcgaagcgc gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc
    18181 ttcttgtaac gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc
    18241 accagtgatc tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc
    18301 gatgacaatc accacgatca gcatgacgta cttccacaaa ggataacggt ttaacacggc
    18361 aattccctta gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc
    18421 agctacgaag tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta
    18481 gccgttttcc gctactttgg ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc
    18541 acctttggca atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg
    18601 caggatcatg aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg
    18661 gctaccttgc gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa
    18721 atttccctca ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct
    18781 ggtaaaaatc agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg
    18841 ccatcaaacg ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt
    18901 cgttgcaacg gtcaagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat
    18961 cacactcagg atcaagtggg ccagtatcgc tcttatactt cgcattgcgg attttcacca
    19021 cgccatcggt cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa
    19081 acatatcgat accacgacgt acgccttcaa ccaggtcttc tggtttacca acgcccatca
    19141 ggtaacgcgg tttgtctgcc ggaatttgcg ggcatacatg ctccagaatg cggtgcatat
    19201 ctgctttcgg ctcacccaca gccagaccgc cgacagcgta gccatcaaaa ccgatatcta
    19261 ccagaccttt aacagaaata tcacgtaaat cttcgtaaac gctgccctga atgataccaa
    19321 acagcgcatt tttgtttccg agactgtcaa aacgctcacg gctacgcttc gcccaacgca
    19381 gagacatctc catggagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat
    19441 caaagatcat gacgatatcc gaaccaagat cgtactgaat ctccattgat ttttcaggat
    19501 cgaggaaaat cggatcgccg ttgatcgggt tacggaagtg cacgccctgt tcggtgattt
    19561 tacgaatatc gccaaggctg aagacctgga agccgccgga gtcggtgagg atcggcccct
    19621 tccactgcat aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca
    19681 gccacaggtg gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg
    19741 gcgtcatccc ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta
    19801 cgccacgatc aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa
    19861 atttcatttt ttctccacta cgtcagaaaa acagtccaac gttttaaacc agcgccacgg
    19921 aattactccc cgacgcgctc attaattgcc tgcggattgt acgtgataaa catcgcatca
    19981 ccgtaactaa aaaagcgata tttctcttct accgctgctt tataggcgtt catggtgtgt
    20041 tgataaccgg caaaggccga aaccagcata atcagcgtcg attccggcag gtggaagttt
    20101 gtcaccagcg catcgaccac tttgtactga aagcccggat agataaagat ttgggtatcg
    20161 tcaaagaacg gttcgataag atcgtttttc gccgcctgag ccgcgctttc cagcgaacgt
    20221 actgaggtgg tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg
    20281 tctaccacat cctgcggcac ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg
    20341 tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa cgccatctcc
    20401 acgcctttgg cgcgtaattt ttccagcaaa ggttcgtcaa aatgcagacc tgcggtcggg
    20461 gctgcaaccg cgcccggttt ttcgctataa acggtttgat aaagttcgcg gtcagcgtct
    20521 tcgtccggac ggtcgatata cggcggcagc ggcatatggc cgatgctgtt gagaatatcc
    20581 agcaccgagc gttcatcatt aaattcgact tcaaacagtg cgccgtggcg cgcggtcatt
    20641 gttgcgttaa tactttcgtc atcgcccagc agcagttctg cgccaggttt tggcgctttc
    20701 gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgttcaac cagcacttca
    20761 atcttgccgc cgctggcttt acgcccaaac aggcgcgccg ggatcacgcg ggtattatta
    20821 aaaaccagaa gatcgccggg gttgagctta tcaagtaaat cggtgaaagt accgtgcgtc
    20881 agcgcgcccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc aggcatggga
    20941 tagtgggcaa tcagggattc gggcaattca aaggagaaat cggtaacgcg catgacactg
    21001 actcagacta aaataagagg cgggtagtct agtgccgggg cgctctccct gcaaccttta
    21061 cccccttttg gatagaatat aatcgatgaa ttttttagct cacctgcatt tagcccatct
    21121 cgcggaaagc tcgctttccg gcaatttact ggctgatttc gtacgcggaa atcctgaaga
    21181 aagttttccg cccgacgtcg tggctggcat tcatatgcat cgacgtatcg acgtattaac
    21241 cgacaatctg ccggaagtcc gcgaagcacg ggaatggttt cgtagtgaaa ctcgccgcgt
    21301 tgcgcctatt acgctggatg tcatgtggga tcactttctt tcccgccact ggtcgcagct
    21361 gtcgccggac tttccgctac aggaatttgt ctgttatgcc cgcgagcaag tgatgacgat
    21421 tttgccggac tcaccgccac gttttatcaa tctgaacaat tacttgtggt cagagcagtg
    21481 gctggtgcgc tatcgcgata tggatttcat ccagaacgtg ttaaacggca tggcaagccg
    21541 ccgcccacgt ctggatgccc tgcgtgactc ctggtacgat ttagacgctc attatgacgc
    21601 cctcgaaacc cgcttctggc agttttatcc gcggatgatg gcgcaggcgt cacacaaggc
    21661 gttataaaac gttagttcat ccataccgta gccgaaatgg caggcaaagc cagaatcccg
    21721 tcagtcagtt gcccatgcct ttctttgcat tgccattgca cggcattgag caacggtgac
    21781 gcgggtagca cgacttcaca agcttcgcca cggttgattg ccaccagtac acgctgctga
    21841 ttcagcacgc ggacaaatac caccacgtta tcttccgcat acagcacctg acagccgcca
    21901 tgacgtagcg cctgactttt cttacgcagc gcaatcattc gctggtacag cgcgaataac
    21961 gccgtatcct gcttttccac ctgccagggg aacggtttac ggcaaaacgg atcgtttttg
    22021 ccatccagtc ctacttcatc accgtaataa atgcacggta caccaggcca ggtgaacagc
    22081 cagaccaccg ccagcggcag gcgcgcaata tcccgaccga gcagcgtttt aaatcgcgca
    22141 gtatcgtggc tgtcgagctg attaaacata cgtaattgtt gttgatgaga aagccctgcg
    22201 cggtaattat ccatccaggc catacaggtt tgggcatcaa tttgctgcgg atcgtaagag
    22261 atatcggtat tggcaagaaa tccccacaac gggaatgtga agccacgata gttcatggcg
    22321 gcatcttcca catcggcctg taaccattgc cgtgcatcgc caaaatgttc gccgacaata
    22381 tacgcttccg gctgggtttc tttcgccgct tcggtgctcc cagcaacgtg ctgcatatta
    22441 ttgcgcgccc cacccgcctc ccctagcata tgcaccacat ccagccgcca gccgtccata
    22501 ctccacggcg ctttcagcca atggcggaca atactgtctt ccccgcgata aatttcattc
    22561 accagacttt ccgactgata atccagcttc ggcaagctgg catagccaag ccagtcgagc
    22621 gccgtgccat catcactaaa cgagtaccag tcgcgccagg gcgattcagg gttgtgacaa
    22681 gctccccccg tgccacgatt gtgcctatca aaccaggcat gggaatcgcc actgtggtta
    22741 aacacgccat ccagccccag ccgcattccc agctgctgcg tattgtggcg taagcgcagc
    22801 aacgccccat caccgccaaa ctgcggatcg acatggcgat aatcctcggt atcgtattta
    22861 tgtacgctgg gagctttaaa caccggattg agatacagcg ccgtcacgcc aagctttttc
    22921 agatacggca gtttttcgct tatcccgtcc agatcgccgc catagaacgt tgatccgccc
    22981 gcctgcgccg tgaccggttc atcccagtca cgcaagatga tctcttgtcc ggctgcatga
    23041 tggtaataga catgatcctg ttcagcttca cgaggaagac tacgcgcaaa acgatcaggg
    23101 aagatctgat aaaaaatctg atccgcagcc cattgtgggc cgatatccgg tacatcgacg
    23161 gcaaactgct ccagtcgtgc cggcggcatt cggctgaagc cctgcggtgt aaaccaacgc
    23221 tggcgatcgt gccacagcag tttgaaactg taacgccgcc ggggttgtcc gctggagaga
    23281 tcaatcgccg cacgccatgc ggtgacgcca ggctgctgct gactgcgatg cttatgcatc
    23341 gatactgaca tttcttcgtt atcgtgttct gtacgcagca taatgcgctg cggtgggtct
    23401 tcgcccgtca gccacagtgt aatgagcagt tgatctttgc tttgtttaac aaatgggggc
    23461 accggcaggt gccatgcatt taacatcata attcccctgt gatgaaaatc tgatcacctt
    23521 gccatagcct aatgcaggat cactcatcat ctgaaggata tttgggggag gaggacgggg
    23581 gcggataact ttcggaatag ccggatcagg atttcctcct gaaccggctt acagaacgca
    23641 aggattactg gttttcagcc agctgacgat cgtggcgacg cttaaacatc cagccaatca
    23701 acagcacaac aatccacgcg aaaccgacat acagcgagat acgcgtatcc gggtgataac
    23761 caatcaaccc gataataaag agcaggaaaa tcagcccgcc gatggtcgtt gctaccccac
    23821 ccggcacttt aaatttcagc gccttaactt cttctggcgg caaacagcga cggaaggcaa
    23881 tttgcgacag caggatcata atccacaccc acaccgtggc gaaggttgcc agcgaagcga
    23941 tcaccaggaa gacgttttcc ggcatgatgt agttcagata caccgcaaac agcagcgcgg
    24001 tagtcatcac cagcaccgta acccacggaa taccgcgacg cgacgttttg ctgaaaattt
    24061 tcggcgcgct gccctgctct gccataccgt ggagcatacg gcctacgcca aagacgtcac
    24121 tgttaattgc cgatagcgaa gcggtcagca caacaaagtt aagaatgcta gcggcaaagg
    24181 taatgcccat atgctggaac gtcagcacga acgggctacc ggcagtgcca acctgattcc
    24241 acgggtagat agacataatg acgaacagcg taccgacgta gaacaccaga atacgcatcg
    24301 gcacggagtt aatcgcacgc ggtatcgatt tctcaggatc tttcgcttca ccggcggtaa
    24361 tcccgataat ttcgatccca ccgtaagcaa acatcaccat ttgcaacgac atcaccatcc
    24421 caagccagcc gttactgaag aagccgccgt tgctccacag attatgaata ccggtcggtt
    24481 gcccgccgtt gccaatcccc cagatgatga tgccgaaacc agcgacaatc atgatgatga
    24541 tggtggcgac tttaaagaac gagaaccaga attccagctc cccgaagacc ttcacgctca
    24601 tcaggtttac ggcgcaaatg atcagcacca cgctcagtac ccaaatccag tgcggcaccg
    24661 tcgggaacca gacacccata tagataccaa aagcggtcac atcggcgatg gcgacaataa
    24721 ggatttcaaa gcagtaggtc cagccggtaa tgtaacctgc cagcgggccg aggttttcct
    24781 gcgcataacg cgagaaagag ctggcggccg ggttatgtac cgacatttcc cccagcgcac
    24841 gcataatgat atacgccgcg ataccaccga taatataggc caacaacacg ctcggaccgg
    24901 ccattttgat ggcgtctgcc gaaccgtaaa acagcccggt accaattgct gaacccagtg
    24961 ccataaagcg tatgtggcgg gtacttagcc cacgctttag cttgttctta ctttccatca
    25021 attcctaacc catcaacaca ataaaaaacc acggggcatc gcagccccgt ggttaaaaca
    25081 aatgttcagt gatttagtga gcgctggagg tcacctgacg acctgccgcg cgatcccaga
    25141 taatggccag aaccaccatc accactgttg gcattaacca cgccagacct tgttcggcca
    25201 gcggtaaacg ctgcgcccag gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt
    25261 cgagaatacc aaaaagcagg ctgataaaca tcggcggagc aatcacgcgg gacgaattat
    25321 gccaccatga gcgtgtaaaa cttaatacaa ccagtgcgat acacggcgga taaatagcgg
    25381 tcagcaccgg tacggagatc tggatcagct ggcttaagcc gaggttagaa accaccatcg
    25441 agaagccgcc gaggataaac accagcgtac gataagagag cggtacgtac tgggcaaaga
    25501 attctgcaca agcacaggtc aggccaactg ccgttaccaa gcaggcgatg aagattaacg
    25561 ccgccagcag gaagctaccg ccgccgccaa aggtgtgctg aacgtaagca tgcagaatag
    25621 cagcgccgtt tgcagactga tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg
    25681 ccaggtacag cagagtcaga ccaacacccg ccatcaggcc agcccagacg gtataacggg
    25741 tcagcagacg cgcttcggta acgccacgag aacgcgccgc gttaacaata acgataccaa
    25801 acaccattgc gcccagcgta tccatggtca gatagccgtt aacgaagccg ttagaaaacg
    25861 cagcgttttg ataagcctca gtcgccgtgc tgatagaacc cgccggccag acaatcgcgg
    25921 caacagacag gatgaccagc gcgataattt tcagcggcgc aaggaagttg cccacggtat
    25981 ccagcagctt gcccggatag agcgaaacca gaataacgat agcgaaatag accaggctgt
    26041 agataaacag cggcagcgcg gaatcaccag tcagcggcgc aatccccact tcaaaggaaa
    26101 cggtagctgt acgcggcgta gcgaaaagcg gccccaccgc caggtaacaa accgttgcca
    26161 gcagcacgcc agcgacttta ccgattggcg tgctgaggct gtcaacaccg ccgccaactt
    26221 ttgccagcgc cactaccgtt aataccggca ggccaacggc agtaatgagg aagccgaatg
    26281 ccgcagtcca gacgtgttcg ccagcctgca agccgaccat tggagggaaa ataatgttac
    26341 ctgcgccgac gaacaacgca aatgtcataa agcccagagc gatgatatcg cgcgatctta
    26401 attgatgggt cataaatcat actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg
    26461 ccaatccctg gggatgatac agcggtatat ttaaacaatt ttagcgggaa atactcccaa
    26521 ccaggcgcgg cgaagaatag tttttcgatt taccgcgtta atgcagtcag tatgacagca
    26581 cctgaagcgc aatttaaacg cttataacgt tttaaagcaa ggagggatct aaaaaccaga
    26641 ggtatatagc gataagaaat caccaaccag tataaaacac tatttccgtg aaaaagacat
    26701 ggctaatcat gcgaacaaaa aatcaccaac cgttgtaaaa tctaacgccc gtattttgac
    26761 tgcaaagaaa ataagccagc atcgactggc ttatggcaat aaaagatgac aaaggcggat
    26821 taatcgctgt ttttggcaat taaacgttcc gggataacaa aactgaaacg tgttcctttt
    26881 cctactgtac tctcaatatt caggcgactt tcgtgatgat tcacagcatg tttcacgatc
    26941 gctaacccta atccgctacc gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa
    27001 cgctcggtca ggcgcggaat atgctccggt gcaatgcccg gtccgttatc ttcaacgcta
    27061 aattcggcac cgtgcggcac tcgctgccag cgtacggtga tatgcgtgcc ttctggagta
    27121 tgattcacgg cgttatagac caggttcgaa atcgcactgc gtagctgatc ttcgttgcca
    27181 gacactttga ggccgttatc tatctcaaag gtaaatgtct gttttttctg actcagagtc
    27241 tgagcctcgc gctcaacaac gcgcagcatc atcggcacat caaccttttc attgagcaat
    27301 tgcgtcggcg cggcttcaat tttcgacagc gttagcaact gcttcaccag cccttccatc
    27361 cgctgggtct gctcgcgcat ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc
    27421 tgctcatcca tcatctccag gtaaccctgt aacacggtca atggcgtacg taactcatgg
    27481 ctcacgttgg caaaaaagtt acgccgcgcc ccttccagtt gatgcatttg cgtgacatca
    27541 cgcgccacca tcagcaactg tttgtgggta taaggcatga cgcgaatttc cagatgccgc
    27601 ccggtgttga gcaccagatt gagcgggcga gaaaaatcac gcgttttcag atattgcgta
    27661 aactccgggt aacgcagtag gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa
    27721 ccaagaattt gttgcgccag accgttacac cagaaaatac cgctctcttc cgtggtcagc
    27781 accaccgcgt cgggcagcga ctccgcgccg ctacgaaagc gtttaatcag attgcccagt
    27841 tcacggcggc gttttttatt tcgcagctgc atctggtgta agccgtatag tagcggttcc
    27901 cagctaccac gccccggtgg cggggtcata ctgcgatcca cccacagcca ccatgaaagg
    27961 cgcaataaat tccagaaatg ccagataagc agtcctgtta ccgatgccag caaaaaccag
    28021 ggcaggtaac caaaaaatgc acccaggatg aaagccggga ggcagcaaag tagcagctcc
    28081 agcaccagcc ttttccacga cagccgttcc agcacgtaag atactcccgt taagaaatca
    28141 taagccctgc tctgcgtccg atgagcaagg cgttaaaagc gggttgaaaa acgatatcct
    28201 gtaccgcgca cggtctgcac catgcggtca tgcccgccgg gctccagtgc tttacgcagg
    28261 cgacgaatgt ggacatcgac cgtgcggtct tccacataca cgttagttcc ccagacgtgg
    28321 tttaacagct gctcgcggct gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt
    28381 ttaaattctg tcggccccat ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc
    28441 gggtcgagac ttaatccctg catctcaatc acctcttcca ccgccattgg cgaaatacgg
    28501 cgcattaccg ctttgattcg cgccaccagc tccttcggcg aaaacggctt ggtgatatag
    28561 tcatccgcgc cggtttcaag gccgcgcacg cgatcttctt cttcccctct ggcggtcaac
    28621 atcaccactg gaatatcccg ggtcatcgac tcgcgcttaa ggtgtttgat gaactggata
    28681 ccggagccgc caggtaacat ccagtcaagg agaattaaat ccggccaggg ttcattcagt
    28741 tgattcacag cacagtcata atcttccgct tcgaccggct gaaagccatt ttgttcgagc
    28801 acgaagcaga ccatttcacg aattggagct tcatcttcta cgaccagaat acgtctcgcc
    28861 atgatttgcc ctgttgtaat aaataggttg cgatcattaa tgcgacgtca ttatgcgtca
    28921 gatttatgac agatttatga aaagctcgtc gcacatatct tcaggttatt gatttccgtg
    28981 gcgcagaaaa aagcaaatgg cacatctgtt tgggtataat cgcgcccatg ctttttcgcc
    29041 agggaaccgt tatgcgcatc cttcacacct cagactggca tctcggccag aacttctaca
    29101 gtaaaagccg cgaagctgaa catcaggctt ttcttgactg gctgctggag acaacacaaa
    29161 cccatcaggt ggatgcgatt attgttgccg gtgatgtttt cgataccggc tcgccgccca
    29221 gttacgcccg cacgttatac aaccgttttg ttgtcaattt acagcaaact ggctgtcatc
    29281 tggtggtact ggcaggaaac catgactcgg tcgccacgct gaatgaatcg cgcgatatca
    29341 tggcgttcct caatactacc gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc
    29401 gtcgcgacgg gacgccaggc gcagtgctgt gccccattcc gtttttacgt ccgcgtgaca
    29461 ttattaccag ccaggcgggg cttaacggta ttgaaaaaca gcagcattta ctggcagcga
    29521 ttaccgatta ttaccaacaa cactatgccg atgcctgcaa actgcgcggc gatcagcctc
    29581 tgcccatcat cgccacggga catttaacga ccgtgggggc cagtaaaagt gacgctgtgc
    29641 gtgacattta tattggcacg ctggacgcgt ttccggcaca aaactttcca ccagccgact
    29701 acatcgcgct cgggcatatt caccgcgcac agattattgg cggcatggaa catgttcgct
    29761 attgcggctc ccccattcca ctgagttttg atgaatgcgg taagagtaaa tatgtccatc
    29821 tggtgacatt ttcaaacggc aaattagaga gcgtggaaaa cctgaacgta ccggtaacgc
    29881 aacccatggc agtgctgaaa ggcgatctgg cgtcgattac cgcacagctg gaacagtggc
    29941 gcgatgtatc gcaggagcca ccagtctggc tggatatcga aatcactact gatgagtatc
    30001 tgcatgatat tcagcgcaaa atccaggcat taaccgaatc attgcctgtc gaagtattgc
    30061 tggtacgtcg gagtcgtgaa cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc
    30121 tcagcgaact cagcgtcgaa gaggtgttca atcgccgtct ggcactggaa gaactggatg
    30181 aatcgcagca gcaacgtctg cagcatcttt tcaccacgac gttgcatacc ctcgccggag
    30241 aacacgaagc atgaaaattc tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga
    30301 atggaagatt gatttcaccc gcgagccgtt cgccagcaac gggctgtttg ctattaccgg
    30361 cccaacaggt gcggggaaaa ccaccctgct ggacgccatt tgtctggcgc tgtatcacga
    30421 aactccgcgt ctctctaacg tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc
    30481 cgaatgtctg gcggaggtgg agtttgaagt gaaaggtgaa gcgtaccgtg cattctggag
    30541 ccagaatcgg gcgcgtaacc aacccgacgg taatttgcag gtgccacgcg tagagctggc
    30601 gcgctgcgcc gacggcaaaa ttctcgccga caaagtgaaa gataagctgg aactgacagc
    30661 gacgttaacc gggctggatt acgggcgctt cactcgttcg atgctgcttt cgcaggggca
    30721 atttgctgcc ttcctgaatg ccaaacccaa agaacgcgcg gaattgctcg aggagttaac
    30781 cggcactgaa atctacgggc aaatctcggc gatggttttt gagcagcaca aatcggcccg
    30841 cacagagctg gagaagctgc aagcgcaggc cagcggcgtc acgttgctca cgccggaaca
    30901 agtgcaatcg ctgacagcga gtttgcaggt acttactgac gaagaaaaac agttaattac
    30961 cgcgcagcag caagaacaac aatcgctaaa ctggttaacg cgtcaggacg aattgcagca
    31021 agaagccagc cgccgtcagc aggccttgca acaggcgtta gccgaagaag aaaaagcgca
    31081 acctcaactg gcggcgctta gtctggcaca accggcacga aatcttcgtc cacactggga
    31141 acgcatcgca gaacacagcg cggcgctggc gcatattcgc cagcagattg aagaagtaaa
    31201 tactcgctta cagagcacaa tggcgcttcg cgcgagcatt cgccaccacg cggcgaagca
    31261 gtcagcagaa ttacagcagc agcaacaaag cctgaatacc tggttacagg aacacgaccg
    31321 cttccgtcag tggaacaacg aactggcggg ttggcgtgcg cagttctccc aacaaaccag
    31381 cgatcgcgag catctgcggc aatggcagca acagttaacc catgctgagc aaaaacttaa
    31441 tgcgcttgcg gcgatcacgt tgacgttaac cgccgatgaa gttgctagcg ccctggcgca
    31501 acattccgag caacgcccac tgcgtcagcg cctggtcgcg ctgcatgggc agattgttcc
    31561 ccaacaaaaa cgtctggcgc agttaatggt cactatccag aatgtcactc tggaacagac
    31621 gcaacgtaat gtcgcactta acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact
    31681 tgccgatgtg aaaaccattt gcgagcagga agcgcgcatc aaaacgctgg aagctcaacg
    31741 tgcacagtta caggcgggtc aaccttgtcc actttgtggt tccaccagcc acccggcggt
    31801 cgaggcgtat caggcgctgg agcctggtgt taatcagtct cgattactgg cgctggaaaa
    31861 cgaagttaaa aagctcggcg aagaaggtgc ggcgctgcgc gggcaactgg atgcattaac
    31921 gaagcagctt cagcgcgatg aaaacgaagc gcaaagcctc cgacaagatg agcaagcact
    31981 tactcaacaa tggcaagccg tcacgaccag cctcaatatc accttgcagc cacaggacga
    32041 tattcaaccg tggctggatg cacaagatga gcacgaacgc cagctgcggt tactcagcca
    32101 acgacatgaa ttacaagggc agattgccgc gcataatcag caaattatcc agtatcaaca
    32161 gcaaattgaa caacgccagc agcagctctt aacggcattg acgggttatg cactgacatt
    32221 gccacaggaa gatgaagaag agagctggtt ggcgacacgt cagcaagaag cgcagagctg
    32281 gcagcaacgc cagaacgaat taaccgcgct gcaaaaccgt attcagcagc tgacgccgat
    32341 tctggaaacg ttgccgcaaa gtgatgatct cccgcacagc gaagaaactg tggcgctgga
    32401 taactggcgg caggttcatg aacaatgtct cgcattacac agccagcagc agacgttaca
    32461 gcaacaggat gttctggcgg cgcaaagtct gcaaaaagcc caggcgcagt ttgacaccgc
    32521 gctacaggcc agcgtctttg acgatcagca ggcgttcctt gcggcgctaa tggatgaaca
    32581 aacactaacg cagctggaac agctcaagca gaatctggaa aaccagcgcc gtcaggcgca
    32641 aacgctggtc acacagacag cagaaacgct ggcacagcat caacaacacc gacctgacgg
    32701 gttggctctc actgtgacgg tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa
    32761 gttgcgtgaa aacaccacga gtcaaggcga gattcgccag cagctgaagc aggatgcaga
    32821 taaccgtcag caacaacaaa ccttactgca gcaaattgct caaatgacgc agcaggttga
    32881 ggactgggga tatctgaatt cgctaatagg ttccaaagag ggcgataaat tccgcaagtt
    32941 tgcccagggg ctgacgctgg ataatttagt ccatctcgct aatcagcaac ttacccggct
    33001 gcacgggcgc tatctgttac agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga
    33061 tacctggcag gcagatgcag tacgcgatac ccgtaccctt tccggcggcg aaagtttcct
    33121 cgttagtctg gcgctggcgc tggcgctttc ggatctggtc agccataaaa cacgtattga
    33181 ctcgctgttc cttgatgaag gttttggcac gctggatagc gaaacgctgg ataccgccct
    33241 tgatgcgctg gatgccctga acgccagtgg caaaaccatc ggtgtgatta gccacgttga
    33301 agcgatgaaa gagcgtattc ctgtgcagat caaagtgaag aagatcaacg gcctgggcta
    33361 cagcaaactg gaaagtacgt ttgcagtgaa ataactattc agcaggataa tgaatacaga
    33421 ggggcgaatt atctcttggc cttgctggtc gttatcctgc aagctatcac tttattggct
    33481 acggtgattg gtagccgttc tggtggttgt gatggtggta tgaaaaaagt cattttatct
    33541 ttggctctgg gcacgtttgg tttggggatg gccgaatttg gcattatggg cgtgctcacg
    33601 gagctggcgc ataacgtagg aatttcgatt cctgctgccg ggcatatgat ctcgtattat
    33661 gcactggggg tggtggtcgg tgcgccaatc atcgcactct tttccagccg ctactcactc
    33721 aaacatatct tgttgtttct ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc
    33781 tcttcgtctt acctgatgct tgccattggt cgcctggttt ccggctttcc gcatggcgca
    33841 ttttttggcg tcggcgcgat cgtgttatca aaaattatca aacccggaaa agtcaccgcc
    33901 gccgtggcgg ggatggtttc cgggatgaca gtcgccaatt tgctgggcat tccgctggga
    33961 acgtatttaa gtcaagaatt tagctggcgt tacacctttt tattgatcgc tgtttttaat
    34021 attgcggtga tggcatcggt ctatttttgg gtgccagata ttcgcgacga ggcgaaagga
    34081 aatctgcgcg aacaatttca ctttttgcgc agcccggccc cgtggttaat tttcgccgcc
    34141 acgatgtttg gcaacgcagg tgtctttgcc tggttcagct acgtaaagcc atacatgatg
    34201 tttatttccg ttttttcgga aacggcgatg acctttatta tgatgttagt tgggctaggg
    34261 atggtgctgg gaaatatgct aagtggcagg atttcaggac gttattcacc actgcgcatt
    34321 gcagcagtga ctgactttat cattgtactg gcactgctga tgctcttttt ctgcggcggc
    34381 atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg cgggattatt tgccctttca
    34441 gcacctctgc aaatattgtt actgcaaaac gccaaaggcg gagagttatt aggtgccgca
    34501 ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg catattgcgg aggtatgatg
    34561 ctgacgctgg ggctggcata taattacgtg gcgctgcctg ccgccctgct ttcgtttgct
    34621 gcgatgtcat cgttgctgct gtatggtcgc tataagcgcc agcaagcggc ggatagtccg
    34681 gtgctggcga aaccactggg gtagattact gtcacggtgc gcgttgctta ttgccggatg
    34741 cggcgtgaac gccttatccg ccctacgcgg ttctggcaca ttttgtaggc ctgataagac
    34801 gcggcaagcg tcgcatcagg catgatgcgc caattgccta cgttttttac tcttgtggcc
    34861 ataaccacgc agcgccgcgt acgccgctgg aatcaccgtg cttcgcctta cgcaccggcg
    34921 tttcacattc gccgccgaag acaaattgtt taatcaactg cccaaccgtt tgatataaac
    34981 ggtctacatt gctcatcccg ccccccagga caatcacatc cggatcgaga atattcacga
    35041 catgtgccag cgattttgcc agccgcagct cgtagcgacg caatgccagt tccgctaccg
    35101 gatcgctttc ttcaaccagg cggataattt cactgccttt cagcgcatgt ccgctcaaac
    35161 gacgataatc cgtcgcgaat cccgtgcccg aaataaaggt ttcaatacaa ccttgtttac
    35221 cgcaataaca agggacttcc tcgcgataac gcagctcgtc ttcatccatc cacggtagcg
    35281 gattgtgtcc ccactcgcct gccgtgccat tgccgccgat atgcgcccgc ccatttaatg
    35341 ccacgcccgc gccgcatccc gtgccgataa tcacggcaaa taccgtctgc gctcccgctg
    35401 ccgcgccatc tactgcttct gaaaccgcca gacagttggc atcatttgcc agccgcactt
    35461 cccgctgcaa cctcgcgctt aagtctttat cgaatggctg accgttgagc caggttgaat
    35521 tggcattctt caccacaccg gtgtagggcg aaatcgaacc tggaatgccc atacctaccg
    35581 ttccgcgctg ccctgtcgcc tgctccgcca tatcaaccaa cgtggcgatc gtttcaatag
    35641 tctgccggta atcatcacgc ggcgtgggca gacgatggcg gtacaactgc tcccctgcat
    35701 cgcccagtgc aatcacttca gttttggtgc cgcctaaatc gatacctata cgcactgtac
    35761 tctccttatt tttttcaata tcaatagcgt agagacggac aaccggattg gcaatgcaag
    35821 gccgccgaca attcgttatc atgcccgcta aatttaacga caaggccgtg gaaattatca
    35881 tgctgtggtt caaaaattta atggtttacc gtcttagccg cgagatttcg ctgcgtgcag
    35941 aagagatgga aaaacagcta gcctcgatgg catttacccc atgcggcagc caggacatgg
    36001 cgaagatggg ctgggttcct ccgatgggat cgcacagcga tgcgttaacg cacgttgcca
    36061 atggtcaaat tgttatctgc gcgcgcaaag aagaaaaaat cctcccgtct ccggtgatta
    36121 aacaggcgct ggaagcgaaa atcgccaaac tggaagcgga acaggcgcgt aagctgaaga
    36181 aaaccgaaaa agattcgctg aaagacgaag tgctgcactc tctgctgccg cgtgctttca
    36241 gccgttttag ccagacaatg atgtggatcg acacggttaa cggtttgatt atggtggact
    36301 gcgccagtgc caaaaaagcg gaagatacgc tggcattact gcgtaaaagc ctggggtcgt
    36361 taccggttgt accattgagc atggaaaacc cgattgaact gacgctgacc gaatgggttc
    36421 gctccggtag tgcggcacag ggcttccagc tgcttgatga ggccgagctg aaatcgttgc
    36481 tggaagatgg cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa gagatcacca
    36541 atcacattga agccggaaaa gtggtgacta aactggcgct cgactggcag cagcgcattc
    36601 agtttgtgat gtgcgacgat ggttcgctca agcgtcttaa gttctgcgac gagctgcgcg
    36661 atcaaaacga agatatcgac cgtgaagatt tcgccctgcg ttttgatgcc gatttcatcc
    36721 tgatgactgg tgaactggca gcgttaattc aaaacctgat tgaaggatta ggtggcgaag
    36781 cacaacgtta attgctgatt ttcctttaat gccggatgcg acgcctgccg cgtcttatcc
    36841 ggcgtacgaa gccacaccag gcatataatt attcgctacg gcgagcaata atttttagcg
    36901 cagcaatatt atgcgtttta cgccgtaact gatctaatcg cctcatccct attcgcctgt
    36961 cagcagcacg cagaatattc actaaccccc atttatcgct ggtaaattgt tttgttaaca
    37021 gctccgcttt tggggtatca atatattcac gcaataaatc agaaattgaa caaatatccg
    37081 tcaggtaagg ataccagcaa gtttcaccgt gaaaattacg cactccagcg ttacctgcaa
    37141 taaagtcttt cgggtaatcc cagatcacgt ttttatccaa cgtgatccaa taacgcggta
    37201 aatcggtact tccaccattt tggctgcgca tgagataacg ggtacagtga atttgcaggt
    37261 tgatcgttgg cgtcagtaag tcatagatct cccgctgtaa cttgctccat ggatgttgtg
    37321 tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga attttccgcg ttgccccggt
    37381 tttcactaaa tcacgtaact gcgaaagatc gggttgttcg acaaataaga gtaatgatag
    37441 atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtggattat taatagccag
    37501 cgaccgatcc tgagactcag tcaatgtcgt taacaacgac gccggggtat gtgcgttgcg
    37561 taatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc accattcatc
    37621 atctgattta tcttttatcg tagccagttc cgcaaaaaat acctggagat cttcactgat
    37681 atttatatca accttgatga attatgaata tatcggaaat tgaaaaaatc gcagcaaagg
    37741 a
//
LOCUS       DAJTXQ010000028        37282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000028 DAJTXQ010000000
VERSION     DAJTXQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.028"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1089
                     /gene="dacC"
                     /locus_tag="OO515_001333"
                     /pseudo
     CDS             1..1089
                     /gene="dacC"
                     /locus_tag="OO515_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1136..1894)
                     /gene="deoR"
                     /locus_tag="OO515_001334"
     CDS             complement(1136..1894)
                     /gene="deoR"
                     /locus_tag="OO515_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6575303.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1952..2548)
                     /gene="ybjG"
                     /locus_tag="OO515_001335"
     CDS             complement(1952..2548)
                     /gene="ybjG"
                     /locus_tag="OO515_001335"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6575304.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2833..4060
                     /gene="mdfA"
                     /locus_tag="OO515_001336"
                     /pseudo
     CDS             2833..4060
                     /gene="mdfA"
                     /locus_tag="OO515_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4101..4385)
                     /locus_tag="OO515_001337"
     CDS             complement(4101..4385)
                     /locus_tag="OO515_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575305.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4471..5286)
                     /gene="ybjI"
                     /locus_tag="OO515_001338"
     CDS             complement(4471..5286)
                     /gene="ybjI"
                     /locus_tag="OO515_001338"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6575306.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5286..6494)
                     /locus_tag="OO515_001339"
     CDS             complement(5286..6494)
                     /locus_tag="OO515_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575307.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6578..7114
                     /gene="rcdA"
                     /locus_tag="OO515_001340"
     CDS             6578..7114
                     /gene="rcdA"
                     /locus_tag="OO515_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6575308.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7289..8974)
                     /locus_tag="OO515_001341"
     CDS             complement(7289..8974)
                     /locus_tag="OO515_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6575309.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9244..9621
                     /locus_tag="OO515_001342"
     CDS             9244..9621
                     /locus_tag="OO515_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6575310.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9651..9908)
                     /gene="grxA"
                     /locus_tag="OO515_001343"
     CDS             complement(9651..9908)
                     /gene="grxA"
                     /locus_tag="OO515_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6575311.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10068..10355
                     /locus_tag="OO515_001344"
     CDS             10068..10355
                     /locus_tag="OO515_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6575312.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10339..11061
                     /gene="nfsA"
                     /locus_tag="OO515_001345"
     CDS             10339..11061
                     /gene="nfsA"
                     /locus_tag="OO515_001345"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6575313.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11122..12024
                     /gene="rimK"
                     /locus_tag="OO515_001346"
     CDS             11122..12024
                     /gene="rimK"
                     /locus_tag="OO515_001346"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6575314.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12112..12588
                     /locus_tag="OO515_001347"
     CDS             12112..12588
                     /locus_tag="OO515_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6575315.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12939..14051
                     /gene="potF"
                     /locus_tag="OO515_001348"
     CDS             12939..14051
                     /gene="potF"
                     /locus_tag="OO515_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6575316.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14146..15279
                     /gene="potG"
                     /locus_tag="OO515_001349"
     CDS             14146..15279
                     /gene="potG"
                     /locus_tag="OO515_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6575317.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15289..16242
                     /gene="potH"
                     /locus_tag="OO515_001350"
     CDS             15289..16242
                     /gene="potH"
                     /locus_tag="OO515_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6575318.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16239..17084
                     /gene="potI"
                     /locus_tag="OO515_001351"
     CDS             16239..17084
                     /gene="potI"
                     /locus_tag="OO515_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6575319.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMVRAEKQRIRDIQ
                     RARHG"
     gene            17144..17632
                     /locus_tag="OO515_001352"
     CDS             17144..17632
                     /locus_tag="OO515_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6575320.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17673..18800
                     /gene="rlmC"
                     /locus_tag="OO515_001353"
     CDS             17673..18800
                     /gene="rlmC"
                     /locus_tag="OO515_001353"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6575321.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18975..19706)
                     /gene="artJ"
                     /locus_tag="OO515_001354"
     CDS             complement(18975..19706)
                     /gene="artJ"
                     /locus_tag="OO515_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6575322.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19998..20666)
                     /gene="artM"
                     /locus_tag="OO515_001355"
     CDS             complement(19998..20666)
                     /gene="artM"
                     /locus_tag="OO515_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6575323.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20666..21382)
                     /gene="artQ"
                     /locus_tag="OO515_001356"
     CDS             complement(20666..21382)
                     /gene="artQ"
                     /locus_tag="OO515_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6575324.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21389..22120)
                     /gene="artJ"
                     /locus_tag="OO515_001357"
     CDS             complement(21389..22120)
                     /gene="artJ"
                     /locus_tag="OO515_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6575325.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22138..22866)
                     /gene="artP"
                     /locus_tag="OO515_001358"
     CDS             complement(22138..22866)
                     /gene="artP"
                     /locus_tag="OO515_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6575326.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23084..23599)
                     /locus_tag="OO515_001359"
     CDS             complement(23084..23599)
                     /locus_tag="OO515_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6575327.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24248..26017
                     /locus_tag="OO515_001360"
     CDS             24248..26017
                     /locus_tag="OO515_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6575328.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            26228..26551
                     /locus_tag="OO515_001361"
     CDS             26228..26551
                     /locus_tag="OO515_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6575329.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26548..27378
                     /gene="amiD"
                     /locus_tag="OO515_001362"
     CDS             26548..27378
                     /gene="amiD"
                     /locus_tag="OO515_001362"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6575330.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(27375..28388)
                     /locus_tag="OO515_001363"
     CDS             complement(27375..28388)
                     /locus_tag="OO515_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6575331.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28487..29917)
                     /locus_tag="OO515_001364"
     CDS             complement(28487..29917)
                     /locus_tag="OO515_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6575332.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29928..30929)
                     /gene="ltaE"
                     /locus_tag="OO515_001365"
     CDS             complement(29928..30929)
                     /gene="ltaE"
                     /locus_tag="OO515_001365"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6575333.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30966..32684)
                     /gene="poxB"
                     /locus_tag="OO515_001366"
     CDS             complement(30966..32684)
                     /gene="poxB"
                     /locus_tag="OO515_001366"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6575334.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32817..33785)
                     /gene="hcr"
                     /locus_tag="OO515_001367"
     CDS             complement(32817..33785)
                     /gene="hcr"
                     /locus_tag="OO515_001367"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6575335.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33797..35449)
                     /gene="hcp"
                     /locus_tag="OO515_001368"
                     /gene_synonym="priS"
     CDS             complement(33797..35449)
                     /gene="hcp"
                     /locus_tag="OO515_001368"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6575336.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(35593..36492)
                     /gene="lysO"
                     /locus_tag="OO515_001369"
     CDS             complement(35593..36492)
                     /gene="lysO"
                     /locus_tag="OO515_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6575337.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36987..>37282)
                     /locus_tag="OO515_001370"
     CDS             complement(36987..>37282)
                     /locus_tag="OO515_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR6575338.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         9287 a         9218 c         9634 g         9143 t
ORIGIN      
        1 cgcgtgcatg gattttaatg gattacgcca gcggtaaagt gctggcagaa ggcaacgcgg
       61 atgagaaact ggatcccgcg agcctgacta aaatcatgac cagctatgtg gttgggcagg
      121 cgcttaaggc cgataagatt aaactcaccg atatggtgac ggtcggtaaa gatgcctggg
      181 cgacgggaaa tccggcactg cgtggttcat cggtaatgtt cctcaaaccg ggcgatcagg
      241 tttcggtggc agacttgaac aaaggtgtga ttatccagtc cggtaatgac gcctgtattg
      301 cgctggccga ttacgttgcc gggagccagg agtcatttat tggtttgatg aatggttatg
      361 ccaaaaaaac tgggtctgac caacactacc ttccagacgg tgcacggtct ggatgcgccg
      421 gggcagttca gtaccgcgcg cgatatggca ttgctgggta aagcattgat ccacgatgtg
      481 ccggaagagt acgccattca taaagagaaa gagttcacct tcaacaaaat tcgtcagcct
      541 aaccgtaacc gtctgctgtg gagcagcaat ctgaatgttg atggcatgaa gacaggaacc
      601 acggcaggtg cgggatataa tctggttgct tcggctaccc agggtgatat gcgtttaatc
      661 tccgtagtgc tgggggcgaa aactgaccgt atccgtttta atgagtctga gaaattattg
      721 acctggggtt tccgcttctt tgaaactgtg acgccaatta aacctgatgc cacctttgtg
      781 actcagcgcg tctggtttgg tgataagagc gaagtgaatc tcggggcagg cgaagcgggc
      841 tccgtgacca taccgcgtgg gcagctgaaa aacctgaagg cgagctacac gttaacggaa
      901 ccgcagctta ccgcaccgct gaaaaaaggt caggttgtcg ggaccattga tttccagctt
      961 aacggtaaat ccattgagca gcgtccgctg atcgtgatgg aaaatgtgga agagggcgga
     1021 ttctttggtc ggatgtggga tttcgtgatg atgaaattcc atcagtggtt cggcagctgg
     1081 ttctcttaat cttctgataa ccggatggcg cgaaacgtca tccggttata cgtcattaat
     1141 acatcaactt aatacggtgc gtctgcgcgt acttcacata ctcatcttcc gggcaacaat
     1201 cactcaccac aatatcaaag cgtttcaggt cacccatgcg cgccggacgc accttgccaa
     1261 atttactgtg gtcgacaacc agcacatgct tttgcgccat cgacatggcc cagtgtttta
     1321 ccggcaactc ttcaagatta aaacaggtag cgcctttact gacatgcacg cccgccgcgg
     1381 aataaaaagc gatatccggg caaaaattat tcaacgtttg ctgaaaatcg atggggttga
     1441 aaatggcgtt gctggcgtga aattcaccac cgcacagaaa tgcgcggcaa tggggtttct
     1501 ctttcagcgc cagaaaggta tttagcgaat aacaaacggc ggtaaaaggg atttcattat
     1561 caatcgcttc aataatccac ggcgtggtgg tgccacagtc aaaaaagagg gtctgatcgg
     1621 gttctaccag tgtcgcagcc agttttgccg cccggcgttt ttcttccacc aggcgggatt
     1681 tttgatcgct taacaggtaa tggctggcac tgcgcggttc cagaacaata tagccgccga
     1741 gcaaaacgac gggcgcactg tggttgttca gatcgcgacg aatcgtcatc tccgaaaccc
     1801 caagcagggc ggcggcgtct ttaagatgta acttatcgct gcgttttaat tcttgcagca
     1861 gctgcccgat acgctcttcg cgacgtgttt ccataatccc tctgaatagt tattgaagcg
     1921 agccgctcaa tactacactt tttagcagag atcagtcacg cacccagcct ttgcggatcg
     1981 gtaatgcaaa acagacgcga taccacgatt gcagacgttg atagagttta tgccccatcg
     2041 cttgccagat aatctgggca ctaaggcagc caatcatacc tgccagcaat ccaccgagca
     2101 tatccagcgg ccagtggacg ccaagataaa cgcgcgacca ggcaatgacg acggccagaa
     2161 ccattaaaag tgagccggac cacaggcgat gccagcataa aaatgccagt gcaaaggtga
     2221 aaatcaccgt accgtgatcg ctcgggaatg agtcatccgc cgcatgatgc aggaagttat
     2281 agccgatatt ttcgacaaag ggtcggtcgt gcggaaaaag atgtcccatc gtccaggaca
     2341 caaacaggct gaccgccagc gcgatagcga tttttatcac cagttgccgt tgtgctgtaa
     2401 gcccccacaa ccaaagtacc gcggccagca acggcaccac ggtaatcaaa tctttagcaa
     2461 taaaaatcgc caacgagatc atccacggag ccgagtctgg cgtcgcgtta ataagagaga
     2521 atagagagag attcaaattt tccagcataa cttcccgacg caaagtgatt aaaagggagc
     2581 caatacaggc aagtcgttga gaataaagtg caggttaaac tgggtaaagc ggcatcgtct
     2641 tatttccctc aagcggcctg tttacggtgg gtgattgtaa cgggcatagg ttaaataaaa
     2701 cttaaagaaa gcgtagctat actcgcaata atgtaagaat gtgcttaacc gtggtttcag
     2761 ctacaaaatt cgctttctcg ttagctgcgc ttttattaaa ctctgcgcga ttattattgg
     2821 cgaagaaatt gcatgcaaaa taaattagct ttcggtgcca ggcttggacg tcaggcgtta
     2881 cttttccctc tctgtctggt gctttacgaa ttttcaacct atatcggcaa cgatatgatc
     2941 caacccggta tgttggccgt ggtggaacaa tatcaggcgg gcattgattg ggttcctact
     3001 tcgatgaccg cgtatctggc gggcgggatg tttttacaat ggctcctggg gccgctgtcg
     3061 gatcgtattg gtcgccgtcc ggtgatgctg gcgggtgtgg tgtggtttat cgtcacctgt
     3121 ctggcaatat tactggcgca aaacattgaa caattcaccc tgttgcgctt cttgcagggc
     3181 ataagcctct gtttcattgg cgctgtggga tacgccgcaa ttcaggaatc cttcgaagag
     3241 gcggtttgta tcaagatcac cgcgctgatg gcgaacgtgg cgctgattgc tccgctactt
     3301 ggtccgctgg tgggcgcggc gtggatccat gtgctgccct gggaggggat gtttgttttg
     3361 tttgccgcat tggcagcgat ctcctttttc ggtctgcaac gagctatgcc tgaaaccgcc
     3421 acgcgtatag gcgagaaact gtcactgaaa gaactcggtc gtgactataa gctggtgctg
     3481 aagaacggcc gctttgtggc gggggcgctg gcgctgggat tcgttagcct gccattgctg
     3541 gcgtggatcg cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa
     3601 tatggcttgc tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg
     3661 cgtctgacct cgcgccgcac cgtacgtgat aattatgggc ggctggccga ttatgattgg
     3721 tctgttggtc gctgctgcgg caacggttat ctcatcgcac gcgtatttat ggatgactgc
     3781 cgggttaagt atttatgctt tcggtattgg tctggcgaat gcgggactgg tgcgattaac
     3841 cctgtttgcc agcgatatga gtaaaggtac ggtttctgcg gcgatgggaa tgctgcaaat
     3901 gctgatcttt accgttggta ttgaaatcag caaacatgcc tggctgaacg ggggcaacgg
     3961 acagtttaat ctcttcaacc ttgtcaacgg aattttgtgg ctgtcgctga tggttatctt
     4021 tttaaaagat aaacagatgg gaaattctca cgaagggtaa aaaaatgcct gactgctttg
     4081 tgcgatcagg cattctcgaa ttaatggtga tggtcgtcaa tctggtgttc gataaccatc
     4141 ccttcaccta cgctggcaag atggcgagca taaggatgcg ggcggtaagc cggagctggc
     4201 gcaggagcca catagacggt ttgcggcaca gtcgcgacgc tgaccgcttg tggaacgctg
     4261 actgaatcag gaactacaac cactttgtat ccactcggca catcgacggt gacactttgc
     4321 gccatcgcca caccagtaaa gcccattaaa agcgcgccca acagtagaca atttttcata
     4381 atcatttcgc ctgaaataat gcagcgggtt aatttccgcg aattatgcag ccttcattca
     4441 ggtattgtgt tgccgggcgt gctgccgttt ttattggtca aatggcgctt cgtgtttaag
     4501 cactttatcg atcacatcca gtacgccttc acggttattg gagcctgccc ggtattttgc
     4561 cgctgcgacg accgcgctgc cggcattttc cattgcaaaa ctaaagcctg cctgacgcag
     4621 catctcaata tcgttaccgc catcgccaaa gaccaccact tcgctgtcgt ctattcccca
     4681 taatttctgc agttggcgaa ggccattggc tttatgtacg ccggggataa tcagatcgat
     4741 gctgccgttg ccggtgtgga ctggcaccat aatatcgccg atggcctcat gtaatgcttt
     4801 ttgtacttgt ggaatcagtt catcggaaag attcaggcca aacttaaaga aaatatcttc
     4861 taagttgtca aagttatcga cgtattccag acggtgataa tacatttctg ccaccgtttt
     4921 catggcatcg tcatattttt tgagcgtata ggcgctattt tttccgcagg caataatttc
     4981 cacttccggg cgcgtcagca aatgttccac gacagtagta aaggcatcct tcgacagctc
     5041 gccattaaaa acatctttgc cttcactcac tacccagccg ccgttttcag ccacaaaggc
     5101 aatttcatta gcaatttcag ggaagaaaga gagcaactga taatattgat tcccgctggc
     5161 gaccacaaag cgaatgcctt gtgctttcat ttgctgatac tgagccataa accgctcacg
     5221 gttataggtt ttttgatcgc ttaagaaagt accatccatg tctaccgcaa ttaatttaat
     5281 gctcatcaac tattctccat cgccgtccgc gttttggtat cgggtttggc gacggctttc
     5341 gcgacaatag ccgcgagaat aaccagcgcc agtacaacca gcattgcact acgtaatcca
     5401 taatgttcgc cgagatagcc cagcagcggc ggcccgacga ggaaagccag ataaccggtc
     5461 gttgctacca cactgacgcg ggttggtgca tcggggccgg tatcgctggc ggcagaaatg
     5521 gtcagcggga agcccagcga ggcacccagt ccccagagta caacagacac cccagcgacc
     5581 caggcgctat cgacaaaaat aatcagccca atacccaacg cccccattag tgcactggcc
     5641 cgaaccacgg caacgcgact gtaacggtcg atgaaccaac cgccagtgaa gcgtccaacg
     5701 gtcatcccta gggtaaaacc ggcataaatc agcgagccgg aagtagggct aaaaccgtga
     5761 ccgtcaacca ttaataaggg taaccagtcg ttggcagaac cttcggcaaa ggccatcgcc
     5821 agcactacaa caccaatcaa cagcaactgg atatcgcgat aaaaaggaat gcctttttcg
     5881 ccatgctggg tgccatcggc agcatttttg cccgtaccgt cagggattgc ctgaatggcg
     5941 atataaatag gcgcgatacc taccagcgcc gccaataaaa tgtgcaccgt tgccggaacg
     6001 ccaaaggccg tcagtgccat cccgacacca gcgcctgcca gcgtgcctag gctataaaaa
     6061 ccgtgcatca tcggcaaaac cgttttattc atttctcgct caacggcggc accttcaacg
     6121 tttatcgcca cttccgcaga accaaaactt gccccaaaga cgccgagacc aacggcaaag
     6181 agcaggggcg atgtcagcca gagtgccaga cttaatatca tcatcccgat caatgcgcag
     6241 gacatcgtga ccaggatgac attacgtgtc ccaaagcgtt tcactaacca cgccgagcag
     6301 agaataccgc tcatcgaacc gatcgacaga ccaaagagaa caccgcccat ttcagcgatc
     6361 gagacagaga gaatatcgcg gatagcaggc gtacgggttg cccaggacgc cattaacagg
     6421 cctggcaaaa agaagaacat aaacagcgcc caggttcggc gtttcaatgc attacgtgaa
     6481 gaatttacgg tcatagatca cgtcaaaata agaagaggga agacaacatt agcaaggttg
     6541 tgtacatttg tacacaattg cagacagagg aaatgacatg cgtcgcgcta acgatccgca
     6601 acggcgagaa aaaattatcc aggccacgct ggaggcggtg aaactttacg gaatacatgc
     6661 tgttacgcac cgcaaaattg ctacccttgc cggggtaccg ttgggatcga tgacctacta
     6721 tttttcagga attgatgagt tgttgctgga ggcgttcagc agttttactg agattatgtc
     6781 ccggcaatat caggcatttt ttagcgatgt tagtgatgct caaggcgcat gccaggctat
     6841 caccgatatg atctacagct cacaggttgc aacgccggat aatatggagc tgatgtacca
     6901 gctctacgcg ctggctagcc gaaaaccgct attaaaaacg gtaatgcaaa actggatgca
     6961 gcgcagtcag caaacgctcg aacaatggtt tgaacccgga accgcccgcg cgcttgatgc
     7021 gtttattgag gggatgacgc tgcattttgt caccgaccgt aagccgctat cgcgcgagga
     7081 gattttgagg atggttgaga gggttgcagg gtagtagata aattttagat aacaaaaaac
     7141 ccatcaacct tgaaccgaaa tggcggggtt gatgggctcc acaaaatggg gacatcaaag
     7201 aaaagcagtg gcactaatta agactgatgc cctgcggaaa agttctgcgg ttgtgcaaaa
     7261 aaatttcatt ttcagggcaa cttcagtttt atcctaatcc tggccatacc atgacgatga
     7321 ttgtccctgc cagcgtcagc aggacgttgg cgattgcgta ggtgcccgca tagcccagcg
     7381 cagggatgtt actgcgagct gtatcactga tgatctccat tgccggcgcg caggtgcgtg
     7441 cgcccatcat tgcgccgaac aacagtgcgc ggttcattcg caatacataa gcaccgaaca
     7501 agaaacagat aaccacgggc acaagactga caattaatcc ggcaatcaac atctgaccgc
     7561 caatcgcgcc caggccgtta ttaataccgc taccggcgct cagaccaacg cctgccataa
     7621 acaccatcaa gccgaactct ttcaccatgc ttaatgcacc ttgcggaatg taaccgaagg
     7681 tcgggtggtt agcacgcata aagcccagca taattccggc gaataacaac ccggcagcgt
     7741 tccccatgcc gaaactgaat gtgctgaact ggaaggtgat catcccgatc atcagcccaa
     7801 taacaaagaa ggcgcaaaat gccagcaggt cagtgacctg gctgtgaatc gagataaagc
     7861 cgatgcgatc ggcgatggtt tttacgcgac gggcatcacc gctgacttgt aaaacgtcac
     7921 ctttgttaag cacgacgttg tcatctatcg gcatctcaat ctggctacga atgacgcggt
     7981 taaggaagca accgtgatcg gtcaacttca gttgtgcgag acgtttacct acagcgttat
     8041 ggtttttaac gaccacttct tcagtgacga tacgcatgtc gagaaggtca cgatcgaaaa
     8101 cttctttacc gttacggaag ctgggatcga gtcgggcatg ggcgtcggga tagcctacca
     8161 acgctatttc atcgcccatt tgtagcacgg catcaccgtc tggatttgcc agaatcccgt
     8221 tacgtcgaat acgttcaatg tagcagccgg tttgtcgata aatacccagt tcacgcagat
     8281 ttttgccgtc ggtccaggcc accagctccg ggccgacgcg ataggcgcgg atcaccggta
     8341 aataaacctt acggttggca tcagtgtcca ggccacgttc gcgggcgatt tgctgggcgc
     8401 tggtctgtaa gtcctgatgc tgcaatttcg gcaagtaacg cgcaccaaca atcaaactca
     8461 ccagaccgat taaataggtt aaggcatacc cgaggctcag attatccagt gccagtgaga
     8521 gctgcctgct ttccatgccg gaatgacgca gtgtatcgcc agcaccgacc agaaccggtg
     8581 tcgacgtcat agagcctgct aacataccgg ccgtcaggcc aatatcccag ccaaacagct
     8641 tacctaaccc taaggcgatc accagcgcac tgccaaccat caccagtgct aacattaggt
     8701 aatttttccc atcgcgaaaa aaaatggaaa aaaagttcgg tccggcttcg accccgacgc
     8761 agaaaataaa cagcataaag ccaagattaa gcgcatcggt gttaatgctg aaatgttgtt
     8821 ggcctaataa cagcgatacg actaaaacgc caatggaatt acccagttgg atcgaaccaa
     8881 gtcgtaactt tccgagacat agcccaagcg tgaggaccac aaataataac agaatgtaat
     8941 tcccatttaa caattcggcg acgtttatat tcacggaggc taacttcttg tttactagta
     9001 agctgttgaa agaaatggta atttacgata atgtttttta ccagaattca gggcgcagat
     9061 tcattcagcg cacctaaacg atagtaaagt aacaatatat tttactagtg taatcacatt
     9121 aggtatcaac ggctatatga attgcgttgg cctatattag catggaatgc gaagcggctt
     9181 tatcttactg aacgccacac tggcgaaaaa tgtgttcgat agacgcagtg tcaggaggaa
     9241 cgagtgaaac ataaacaacg ttgggcgggg gcaatctgct gttttgtcct cttcattgtg
     9301 gtgtgccttt ttctggcgac gcacatgaaa ggcgcttttc gggctgccgg gcatcctgaa
     9361 atcggcttgc tgtttttcat tcttcctgga gcagttgcca gtttcttttc acagcgtaga
     9421 gaagtcctga aacctctatt tggcgcaatg ctggcggcac cctgttcaat gctcattatg
     9481 cggctgtttt tttcaccgac gcgctcattc tggcaagagc tggcatggtt actaagcgcg
     9541 gtgttctggt gtgcgctggg ggcactgtgt ttcttattta tcagtagttt gtttaaacca
     9601 cagcacagaa aaaatcagta aagccctcaa cgcgagggct tgtcagacga tcaggcgtcc
     9661 agattttctt tcacccatgc agcaaaatcg gtatagccgc cgatatgttg ctgatcgaca
     9721 aaaatctgcg gcacggtttc tacgggttta cctgcctttt gttgtagatc ttctttagtg
     9781 atcccttccg cacgaatatc tacatactga tactgaaaat catcgcgttc attgctcaat
     9841 ttctcagcca gatcttttgc acgcacacag taagggcaac ccgaacgacc aaaaataacg
     9901 gtttgcatta tttctctcct catagattta tgcctgtaat gatcacgcta aaatgtattc
     9961 gctgaaagta ggtttaacct gttgcattaa ttgctaaaag ctataactgt taaacacaat
    10021 acagtgaaaa gttttagact gaaggctcac tttgcagagg gaagcgtatg cgcgcgatcg
    10081 gtaaattgcc taaaggcgtg ttgatactgg aatttatcgg aatgatgcta ctggcggtgg
    10141 cgctgctgtc ggtaagcgac tccctgtcgc tgcctgagcc attttctcgg ccagaagtgc
    10201 agattctgat gatttttctc ggtgttttgc tcatgcttcc cgctgcggtg gtggttattc
    10261 ttcaggtggc aaaacgtctt gccccacagc tgatgaaccg tccaccgcaa tattcacgtt
    10321 cagaaagaga aaaagataat gacgccaacc attgaactta tttgtggcca tcgctccatt
    10381 cgccatttca ctgatgaacc catttccgaa gcgcagtgtg aggcgattat taacagcgcc
    10441 cgtgcgacgt ccagttccag ttttttgcag tgtagtagca ttattcgcat taccgacaaa
    10501 gcattacgtg aagaactggt gacgctgacc ggcgggcaaa aacacgtagc gcaagcggcg
    10561 gagttctggg tgttctgtgc cgactttaac cgccatttac agatctgtcc ggatgctcag
    10621 ctcggcctgg cggaacaact gttgctcggt gtcgttgata cggcaatgat ggcgcagaat
    10681 gcattaaccg cagcggaatc gctgggattg ggcggggtat atatcggcgg cctgcgcaat
    10741 aatattgaag cggtgacgaa actgctgaaa ttaccgcagc atgttctgcc gctgtttggg
    10801 ctgtgccttg gctggcctgc ggataatccg gatctcaagc cgcgtttacc ggcctccatt
    10861 ttggtgcatg aaaacagcta tcaaccgctg gataaagacg cactggcgca gtatgacgag
    10921 caactggcgg aatattacct cacccgtggc agcaataatc gccgggatac ctggagcgat
    10981 catatccgcc gaacaatcat taaagaaagc cgcccattta ttctggatta tttgcacaaa
    11041 cagggttggg cgacgcgcta aaaccgccac gtcgatgtat gatacgcggg cttttgacca
    11101 ggtctgacag agaggtgcag ggtgaaaatt gccatattgt cccgggatgg aacgctctat
    11161 tcgtgtaagc ggctgcgtga agccgctata cagcgcggtc acctggttga aattcttgat
    11221 ccgctttctt gctacatgaa cataaatcct gcggcgtctt ctattcacta caaaggccgc
    11281 aagttacccc attttgacgc agtgatcccg cgtattggca ccgccattac cttttatggg
    11341 acggcggcac tgcgccagtt cgagatgctg gggagctatc cgctcaatga gtcggtcgcc
    11401 attgcccggg cgcgtgacaa attgcgttcc atgcaactgc tggcgcgtca gggcatcgac
    11461 ctgcctgtca cgggcattgc gcattcgccg gatgatacca gcgatttaat cgacatggtc
    11521 ggtggtgcgc cgctggtggt caagttggtt gaaggcacgc agggaattgg cgtcgtgctg
    11581 gcggagacgc gtcaggcggc ggaaagcgtg attgacgctt tccgcggtct gaacgcgcat
    11641 attctggtgc aggaatatat caaagaggcg caagggtgcg atatccgctg tctggttgtt
    11701 ggcgatgaag tggtcgctgc gattgaacgg cgggcgaaag agggcgattt tcgttccaat
    11761 ttgcatcgtg gcggcgcggc aagcgtcgcc agtatcacac cacaggagcg tgaaatcgcg
    11821 ataaaagccg cgcgaacgat ggcgctggac gttgctggtg tggatattct gcgtgctaat
    11881 cgcgggccgt tggtgatgga agtgaatgcg tcgccggggc tggaaggaat agaaaaaacc
    11941 actggtatcg acatcgcggg taaaatgatc cgctggatcg aacgccacgc tacgacagaa
    12001 tattgcctga aaacgggtgg ttagtcgcaa tcacattact gatcatggtt ttgcctgcgc
    12061 tttttgcgta agctgtgccg gtctttttat cgaaagaggt tgtacaaaat tatgacatcg
    12121 ctggtcgttc ctggtctgga tacgctgcgt caatggctcg atgacctggg gatgagtttt
    12181 tttgaatgtg ataactgtca ggctctgcat ctgccccata tgcagaattt cgacggtgtc
    12241 tttgatgcca aaatcgatct gatcgataac acgatcctgt tttctgccat ggcggaagtc
    12301 cgaccttcag ccgtattgcc gctggcggcg gatttatctg ccatcaatgc cagttcgctg
    12361 accgtgaaag catttcttga tatgcaggat gataatctgc caaagctggt ggtttgccag
    12421 tctttatccg ttatgcaggg cgtaacctat gagcagtttg catggttcgt gcgtcagagc
    12481 gaagagcaga tttcgatggt cattcttgaa gctaatgccc atcaactgct gttaccgact
    12541 gacgatgaag ggcaaaacaa cgttaccgaa aactatttcc tccactgata actcctttcg
    12601 agcacgcagt cgctggtgca gtggctgcgc gctgcaaaat tatctgctgt ttttaacctt
    12661 ttcttaaaga ttatttcact tctcttgtgt cgatttggct ttatcacata gagcaaatac
    12721 gcataaaaat ttgttaaata ccgtttttta atccgagcta tagtctcaaa ccctggctaa
    12781 agttattctt gcgatgcttt tatatagcga gcagtgctgg ccgggagaga gttctctttt
    12841 cttacacccc gccgataaaa aatatgcacg tttattgcat atcttttagt gtgacaactt
    12901 ttgttcgttt gttaacgaac tttcagaagg aaagagatat gaccgcctta aataaaaaat
    12961 ggctttcggg tctggctgcg ggtgctctga tggccgtctc tgtcggcacg ctcgcggctg
    13021 aacaaaaaac actccacatt tataactggt ctgattatat cgccccggac acggtggcca
    13081 attttgaaaa agaaaccggt attaaagtcg tctacgatgt tttcgactct aacgaagtac
    13141 tggaaggcaa attaatggcg ggtagcaccg gctttgactt ggtggttcca tctgccagct
    13201 ttcttgaacg ccagttgact gcgggagttt tccagccgct ggacaaaagc aaattgccgg
    13261 agtggaagaa tctcgacccg gagctgctga agctggtcgc caaacacgat cccgacaata
    13321 aatttgctat gccctatatg tgggcgacga ccgggattgg ctataacgtt gataaagtta
    13381 aagctgtgct gggcgaaaac gcgcccgtcg atagctggga cttgatcctc aaacctgaaa
    13441 atctggaaaa actgaaaagc tgcggtgtct ctttcctgga tgcgccagaa gaagtttttg
    13501 ctaccgtgtt gaattatctc ggcaaagatc ccaacagcac taaagcggat gattacaccg
    13561 gaccggcaac cgatctgctg ttaaagctgc gcccgaacat tcgttatttc cattcatctc
    13621 aatacattaa cgacctggca aacggcgata tttgcgtcgc tatcggctgg gcaggtgatg
    13681 tctggcaggc gtcaaaccgc gcgaaggaag cgaagaatgg agtgaatgtc tcgttctcga
    13741 ttccaaaaga aggggcgatg gcgttctttg atgtattcgc catgcctgcg gatgccaaaa
    13801 acaaagacga agcctatcag ttcctgaatt acctgctgcg cccggatgta gtagcgcata
    13861 tttccgacca tgtgttctat gccaacgcca ataaagcagc cacgccgctg gtgagtgcgg
    13921 aagtccgtga gaacccgggt atttatcctc cggcggacgt tcgtgcgaag ctattcactc
    13981 tgaaagtaca ggatccgaaa atcgaccgtg tgcgcacccg cgcgtggacc aaagtgaaga
    14041 gcggaaaata atccgcagcc ttagatgccg ggcgggcgca ccacaccagc cggcaattcg
    14101 caccatcatg gtgcgcttgc acacattcaa tgccggagag cagccgtgaa tgacgctatc
    14161 cctcgcccgc aggcgaaaac ccgtaaggcg ctgacgccgc tattagaaat ccgcaacctc
    14221 accaaatcct acgatggtca gcatgcggtc gatgatgtca gcctgaccat ctacaaaggt
    14281 gaaatcttcg cgctgctggg cgcatccggc tgtggcaagt ccacgctgct gcgtatgctg
    14341 gcaggttttg aacaaccttc tgccgggcag ataatgctcg atggcgtcga tttgtcacag
    14401 gtgccgcctt acctgcgccc catcaatatg atgtttcagt cttacgcgct gtttccgcat
    14461 atgaccgtgg aacagaacat cgcttttggc ctgaaacagg acaaactacc gaaagcggaa
    14521 attgccagtc gggtccatga gatgctcggc ctggtgcaca tgcaggagtt cgccaaacgc
    14581 aaaccgcatc agctttccgg tggtcagcga cagcgtgtgg ccctggcccg aagccttgcc
    14641 aaacgcccga aactattact gctcgatgag ccgatgggcg cgctggataa aaagctgcgt
    14701 gaccggatgc agcttgaagt ggtggatatt ctggagcgcg tcggtgtgac ttgtgtgatg
    14761 gtcacccacg atcaggaaga ggcgatgacc atggcggggc gtatcgccat tatgaatcgc
    14821 gggaaatttg tccagattgg cgagccggaa gagatctacg agcatccaac tacccgctac
    14881 agcgctgaat ttatcggttc ggttaatgtc tttgaaggtg tactcaaaga gcgtcaggaa
    14941 gatggcctgg tgcttgattc gccggggctg gtgcatccac tgaaagtcga tgcggatgct
    15001 tcagtggtcg ataacgtgcc ggtacacgta gcgctgcgcc cggaaaaaat catgctttgc
    15061 gaagagccgc ccgccaatgg ttgtaacttc gcggtggggg aggtgataca cattgcctat
    15121 ctcggcgatc tttcggtgta tcacgttcga ctgaaaagtg ggcagatgat cagcgcccag
    15181 ctacaaaacg cccatcgtca tcgtaaaggg ttaccgacct ggggcgacga agtgcgtttg
    15241 tgctgggaag tggacagctg tgtggtgctg acggtttaag gagcaaagat gagtacactt
    15301 gaacctgctg cccagtcgaa accgccgggc ggatttaagc tgtggttgtc gcagctgcaa
    15361 atgaagcatg ggcgcaaact ggtcattgcg ttgctatata tctggttgat cttgctgttt
    15421 ctgctgccat ttctgattgt ctttaaaata agcctgggag agatggcgcg cgctattcca
    15481 ccttataccg aactgatgga gtgggctgac gggcaacttt ccatcactct taatctcggt
    15541 aattttctgc aactgaccga cgatccgctc tattttgatg cttatctcca gtcgttacag
    15601 gtggcggcga tttcgacatt ttgctgttta ctgatcggct atccgctggc gtgggcggtg
    15661 gcgcacagta agccttcgac ccgtaatatt ttattactac tggtgatcct gccgtcgtgg
    15721 acctcgtttc tgatccgcgt ttatgcctgg atgggaatat taaaaaacaa cggtgtgctg
    15781 aataattttc tgctgtggct gggggttatc gatcaaccgc tgaccattct gcataccaat
    15841 ctggccgttt atatcggcat tgtttacgct tacgtgccgt ttatggtact gccgatttat
    15901 accgcgttga ttcgtattga ttattcgctg gtggaagcag cgctggatct cggtgcacga
    15961 ccgctgaaaa cgttctttac tgtgatcgtg ccgctgacta aaggtgggat tattgccgga
    16021 tcgatgctgg tgtttatccc ggctgtgggc gagtttgtga tcccggaact gctcggtggc
    16081 ccggacagca tcatgatcgg gcgcgtgtta tggcaagagt ttttcaataa ccgcgactgg
    16141 ccggtggcct cggcggtagc gatcatcatg ttgctgctgc taattgtgcc gataatgtgg
    16201 ttccacaaac accagcaaaa aagcgtggga gaacacggat gaataattta ccggtagttc
    16261 gttcgccctg gcggattgtg attttgctgc tgggctttac ctttctctac gcgccaatgc
    16321 tgatgctggt tatctattcg tttaacagct cgaagctggt gacggtgtgg gccggctggt
    16381 caacgcgctg gtatggtgag ttattgcgcg atgacgcgat gatgagtgcg gttggtttaa
    16441 gcctgacaat tgctgcctgt gcggcaacgg cggcggcgat cctcgggact attgcggcgg
    16501 tggtgctggt gcgttttggc aggtttcgcg gatcaaatgg ttttgccttt atgatcaccg
    16561 cgccgctggt gatgccagat ggcatcacgg gcttgtcgct gttgttatta ttcgtcgcgc
    16621 ttgctcatgc cattggctgg cctgcggacc gcggtatgct caccatctgg ctggcgcatg
    16681 tcactttttg tacggcttat gtggcggtcg ttatttcttc aagattgcgg gaactggatc
    16741 gctcgataga agaagcagcg atggatctcg gtgcgacgcc gctgaaagtg tttttcgtca
    16801 ttacgctacc gatgatcatg cccgcgatca tttctggctg gttactggct tttactttgt
    16861 cgcttgatga tctggtgatc gccagctttg tttccgggcc gggagccacc acgttaccga
    16921 tgctggtctt ttccagcgtg cggatgggag tgaatccgga aatcaacgcc cttgcaacgt
    16981 taattctcgg tgcggtcgga attgtcggat ttatcgcctg gtatctgatg gttcgcgcag
    17041 aaaaacagcg gatacgcgat atccagcgtg caagacatgg ctgaagacac taaaatttgc
    17101 caacctggct acataatgcc gcgcatgccg cggcattgtt ttcatggaag acgaaacgtt
    17161 gggatttttt aagaaaacat cttcatctca tgctcgcctg aatgtgcctg cgctggtgca
    17221 ggtggcggcg ctcgccatta ttatgatccg tggcctcgac gtgctgatga ttttcaatac
    17281 gctgggcgtg cgcggtattg gcgagttcat tcatcgcagc gtacaaacct ggagtttaac
    17341 gctggtcttt ttaagcagtc tggtgctggt tttcattgag atctggtgtg cgttttcact
    17401 ggtgaaaggg cgtcgctggg cgcgctggct atatctgctg acacaaatca ccgccgcaag
    17461 ttacttgtgg gcggcttcgc tggggtacgg ttatccggag ctgttcagca ttcccggtga
    17521 atcaaaacgt gaaatcttcc atagcctgat gctgcagaag ctgccggata tgctcatcct
    17581 gatgctgctg ttcgttccct cgaccagtcg gcggttcttc cagttgcaat aatgtgtata
    17641 atcgtcgccc ctgatgatgt gaaggtcaat gtatgcagtg cgcactttac gacgcgggtc
    17701 gctgtcgttc ctgtcagtgg ataacgcagc cgattccaga gcaactctcc gctaaaaccg
    17761 ccgatcttaa aaatctgctc gccgattttc cggttgagga atggtgcgcg ccggtgtcag
    17821 gcccggaaca agggtttcgt aataaagcca aaatggtggt gagtggtagc gttgaaaaac
    17881 cactgctcgg tatgctgcat cgcgatggca cgccggaaga cctttgtgac tgcccgcttt
    17941 atcctgcctc ttttgcgccc gtttttgcgg cgctaaaacc gtttatcgcc cgtgcggggt
    18001 taacgcccta caacgtggcg cgtaagcgtg gcgaactgaa atacattctg ctgactgaaa
    18061 gccagagcga tggcggcatg atgctgcgct ttgtactgcg ttctgaaacc aaactggcac
    18121 aactgcgtaa ggcgctgccg tggttacagg aacaactacc gcaactgaaa gttattaccg
    18181 tcaatattca gccggtacat atggcgatta tggaagggga gacggagatc tacctgactg
    18241 aacaacaggc gctggcagag cgttttaatg acgtaccgct gtggatccgt ccgcaaagtt
    18301 tcttccagac taatccggcg gtcgccagcc agctgtacgc taccgcgcgc gactgggtac
    18361 ggcagctgcc agttaaacat atgtgggatc tgttctgcgg tgtgggtggc tttggtttac
    18421 actgcgcgac gcctgacatg cagttaaccg ggatcgaaat tgcatcagag gccattgcct
    18481 gtgcaaagca gtcagccgct gaactgggct taacgcgttt gcaatttcag gcgctggact
    18541 ccactcagtt tgccaccgct cagggggatg tgccggagct ggtgctggtt aacccgccgc
    18601 gccgcggcat tggtaaaccg ctgtgtgatt atctctcaac gatggcaccg cgttttatta
    18661 tctactccag ctgtaacgcc caaaccatgg cgaaagatat ccgcgaactg ccaggttacc
    18721 gcatcgaacg ggtacagctt tttgatatgt tcccgcacac cgcgcactat gaagtgatga
    18781 cgctgctggt gaagcaataa aaaagccgca ggtgcggctt cagattgctg atcaggtgcg
    18841 ctttgttcat gccggatgcg gcgtgtacgc cttatccggc ctacaaaatc atgctaaatc
    18901 aatacattgc agagttgatg tgggccagat aagcgcagcg catcaggcaa tcttactttt
    18961 gtcatcagtc tcaattactg cgggaaccac tggtcattga ttttctgata tgtaccgtca
    19021 gctttaattg ctgccagcgc gttattcagt ttttccagca gagctttgtt atccggacgt
    19081 acagcgatgc ccagaccggt gccgaagtat tgcggatcgg tcactttttc agtagcaacg
    19141 cccagttgcg gattggtttt cagccattcg tttaccaccg ctgtgtcacc aaatacccca
    19201 tcaatacgac catttttcag atcgataaag gcattctgat aactgtcata ggagacagtt
    19261 ttcacttcgg ggtgctgatc ctgaatatat ttctggtgcg tggtgccgtt ttccatccca
    19321 atacgtttgc ctttcagatc ggcaaacgtt ttgtaggtat cttttttggc aatcacgacg
    19381 gctgagtttt catagtaggg cgtggtaaac gatacctgct tgctacgctc gggcgtgata
    19441 tccataccgg agattacgga gtcatatttt ctgaatttca gtgacgggat caggctgtcg
    19501 aacgcgtgat tagtaaaagt acattctgcc tgcatttgtt tgcacaaggc ttttgccaga
    19561 tcgatatcaa agccgacaat ctcattatta gcacctatag attcaaaggg tggataggtg
    19621 gctgaaacgc caaaattgat tttctctgcg gcagaagcac cgaaagtaaa ggaagcaagt
    19681 aaagcggcaa gaactaactt tttcatgatg gaactcccgt ctgtcaatct tatgattttt
    19741 ggccgtgtct gcggcatggg ataacaatgc catcaagtga atttatatgc aataaatatg
    19801 attaaataat ttaaatgaaa taaaaaaaga cggataactt agtgagttgt ccgtcttcat
    19861 tataagaatt tatgcactat gtaggccgga taaggcgtcc acgccgcatc cggcacaggc
    19921 accgtgctga tgtctgatgc gacgctggcg cgtcttatca gacctacaaa cccccccggc
    19981 gaatgtacgc agctacatta attccgccgt tcgaatgcca gtgctttgcg ctcgatcaga
    20041 cgcatcatca gcgtcagcag gccgttgacg accaggtaaa taatccctgc cgcaccgaac
    20101 accattacat cgtaggtgcg tccgtacaac aactggctgt atcccatcac ttccatcagc
    20161 gtaatggtgt aagccagaga ggtacttttg aacaccagca ccacttcgtt ggaataagaa
    20221 gagagcgagc gtttaaaggc atacggcagc aggagcgcca gcgtatcttt tttgctcatt
    20281 cccagggcgc tacaggactg ccactgacct tctgggatcg cacgaattgc accgtaaaac
    20341 agctgcgtgg tatacgccgc actgttcagc gataacgcaa tcagcgcaca taaccacggt
    20401 tctgacaaca aatgccacag tgccggatac tcctgcaacg tcggaaactg gcccggcccg
    20461 taataaatca ggaagatctg caccagcagc ggcgtaccgg taaacagcgt gatataaccc
    20521 cgcaccagcc acaccagcac cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc
    20581 agaatcagtg ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg tagccctttc
    20641 atcagttcgg gtaaatactc aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca
    20701 ggtcaatgcg tttgagaatg tactgactga gcagggtgat caccaggtaa atcgccgccg
    20761 ccacaatgta ccaggtaaat ggttcctggg tacgagtagc gatgcttttt gtttgcagca
    20821 ttaaatcatt cacactaatc aaactgacca gcgcggtatc tttcagcagc accagccact
    20881 ggttaccgag gccaggcagc gcatgacgcc acatctgcgg catcaccaga cggaaaaaga
    20941 tagccgattt cgacagcccc agcgcctgac cagactccca ctgacccacc ggcaccgctt
    21001 tcaacgcgcc ccgaagcgtt tgcgaggcat aggcggcata cagcagtgac agagcgatga
    21061 caccacaaag gaacgggcta acgtcgaagt tctcaatgtc catctgcact gggatctgca
    21121 cgaacccaag attgatagtg aagccatccg aaagcgtcag cagcagctgc gaggagccaa
    21181 aatagataaa cagcaccacc agaatttctg gcaggccacg cagaatggtt accagcgctg
    21241 aacctgccca cgcgacagga cgccattttg ccgactccca taccgcaaag aacatcgcca
    21301 gcgccagccc gacaatcaat gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg
    21361 ctaaaggaaa aaattcattc atcaggaatt acttctggaa ccatttgttg tagatggttt
    21421 cgtaagtgcc atctttcttc actttttcca gcgcagtgtt gagtttctgc tgcagctcag
    21481 tgttgccctg acgtaccgcg atgccgaggc cagtgccgaa gtaatcttta tcggtcactt
    21541 tgtcgcccac cgccgccagt ttcgggttat ctttcagcca ctcagtgacc actgcggtgt
    21601 caccgaagac gccgtcgata cgcccgtttt gcagatccag ttttgcgttc tggtagctgt
    21661 catacggaac ggtagtgatt tccgggtgct tatccataat gaatttctgg tgtgtcgtcc
    21721 cgttctgtac gccgactttt ttgcctttca gctgatcaac actggtgtat ttgccttgct
    21781 gacccacaaa cagggcagag ttgtcatagt acggggtggt aaacagcacc tgcttttcac
    21841 gctccggagt gatatccatg cccgccatca cggcttctac gcgacggaat ttcaggcttg
    21901 ggatcaggct gtcaaacgcc tggttagaga aagtgcaggt tgcatcaatc tctttacaca
    21961 gcgcttgtgc caggtcgacg tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa
    22021 acggaggata ggatgcttcg gtagcaaaac gaatggtttc ggcagctgtg gcggaaagac
    22081 taaaacctgc aattaacgcg gcaatcagaa cttttttcat tgttgttatc ccgaatctta
    22141 gtgagagaga tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc
    22201 ttgttctacg atatgaccat tttccatata caccactcgg ctggcggttt tacgcgccac
    22261 ttcaacttcg tgggtgacga tcacctgggt aatattcgtt tctgccagct cacgaatgat
    22321 gctgacgatt tgtgccgtaa tttccgggtc cagtgcggcg gtcggttcat cgaacagcag
    22381 tacctgcggt tccatcatca acgcacgggc aatagcaaca cgctgctgct gaccaccaga
    22441 aagatgcagc gggtaacgat cgctataagg tttgagacgc agacgttcca gcagtttttc
    22501 tgcacgggcc agcgcctgat ctttactcaa ccccagtaca cggcagggcg cttcaatcag
    22561 gttttgctgc acggtcagat gcggccacag gttgtattgc tgaaacacca tgccaacgtt
    22621 acgacgcaaa tcgcgaatcg ctttgtcaga gggtgttttg gtgaaatcga aatggttgcc
    22681 tgcaatgttg agcgtaccgg agcgtggcat ctcaagcaga ttgagtacac gcagcagcga
    22741 gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag
    22801 cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat
    22861 actcattgac actcgtatac tggcagtctg atagctattg aggtcgaaga tagtaccttt
    22921 gacagaataa ttatgcaata tttctgcttt aaaagttaaa agcaaagcgc attattcaat
    22981 aaacatagca caaaataacg ggggcggtgg tcggcgagca taaatgtcgg cattcctcac
    23041 gaaatgccgg acaatttacg gggtttattg gttgatcaag gcgttagcga ttctcgatgg
    23101 actgacggag cgtacccgcc gtggcatgaa cgctgccgcc taagtagcgc acatcgtcga
    23161 tgacccaaca ctggccttcc tgaatcatta acacttcatc ctgccaaccc tggtcaccct
    23221 gtttgagatc tacgcgcaat ggaatgttac gggcatcacg attagggata gtcgatgcac
    23281 tggcaacgtg ggcgctatct ggcaaggtgg ttcgactgga gaacggatcg ctgcttaaca
    23341 attcacgatg actattatcg cggctggcat cgctaagaag ggttgccagt ttgtcactca
    23401 aataggggcg cagggcggta atgtcattgc tgcgatgttg aatgcggtag tcatagaatt
    23461 gttgagccac gctgtcaggc cccccctgga cacaggaacc ggtgcgggga ccgttatctt
    23521 tataagctgg cgtgacggtg gtacaggcac tcagcagcag tgcacatgga ataagtagag
    23581 atagtgaacg gtagcgcata atcatttcct tattagcttg ctgtctgcgt cacactacga
    23641 ccaaacgcta tcagattatt taatacattg tttttaatac aattctgaaa caatctgaac
    23701 acctgattgg gtttgagtcc attgctggct atccactcaa ggttgctcgt agctgactta
    23761 cttaaattac ccaaacaata gacagtgtaa accatttgtg aggttgagcg tgttatatgg
    23821 ctgaaatgat catttaaggt aaatatgata gttctgaaat ggtggtataa atcctcctgt
    23881 agaggttaag agcatctgca agaagccagt ttcacattta taacgcgtga taaatgaacg
    23941 cggttattgt ctaatcatta caaaattttt cacatgcgat aagagatcct gcgattaaga
    24001 tgattgccaa aattacgttg tatttttgaa aatgagcgaa atttgatagt cccagctttg
    24061 actctcgata atattattta tgctacaatt gtggctaaag aatttaagct aatgaatttt
    24121 agcgaagaat aaattaagtt gtttcagtat ctcgttaatt aatatgaata taaaaggcgt
    24181 tgtgctttta taattataaa gcacgaggag ttcggtggat tatcgttttt cttgggtgat
    24241 ttttaatatg ataaacaata tatctgatca agcatcatca tttccaggca cacaactaaa
    24301 tcaaagtgat aatttcctgg attcactgag ggaatttttc gcgatattaa attccagtcg
    24361 taagggtgag ttgtctacat gggataccat ctacttgcat ctgatattag caataaatgc
    24421 tgacagtgat ctcataaaaa atgatgtgtt gctcgcggaa aatatgccat cagcgaatta
    24481 ccaatttaat acttttttta gtaatgcttt cgagactgat gttaaaaaat atctgggtaa
    24541 atcagaagat aacgaagttg aaataaaggc gggtaatgaa agaatctcta ttggaatacg
    24601 taatgtatcg aatgggaaac ttgaaagaca acagtttctt ttccctctgg actatgagaa
    24661 taaattacaa gatcaattag acaagcattt tacgattgaa tcgcacccat tattatacag
    24721 gcataccatt ggtagtaaaa ttgcgaatgt gatttttgaa aaattataca gccgtattga
    24781 ctttaacaaa gaacaatata tatcctttat aaagggcgcg tttattcatt tttatgatta
    24841 tagcaggcga tatgcaatta gtgagaatat cgaaaaagac gctgtaacaa acaatatcgc
    24901 attgatgaac actttttatg acagtgataa tacgtcaggt gaggttttaa ataatgattt
    24961 tacagaagaa gagccatttg aaactgcgtt agatgtagag catacaatag tgcttggatt
    25021 tgctgatgat aattttgaaa caaaacctgt tcattatcag gatttattaa cacgatttag
    25081 tgcctttcaa gataccgtgt ttaatctgtt tcctgaaatg cgctcttcgc attaccacga
    25141 tatttgtagc gtatgtgtgg atatgacaaa gggaacgcaa tgcatgattc acttgatggt
    25201 gaatgaagaa gttttcatgt ctctgcctgt tccagttgcc acgatggtac gtgaagatgc
    25261 aagtaattta gtcaatctta aaacactact caatgatggt tttttcatta aatatagtca
    25321 ttttaatgac gtggcattaa taaagcaaaa cataagtaat ctctacttat cacacactgt
    25381 tgtgaatgaa agcatattga aaaaatgttg ttttgaaaac ggcagtttag gtgatgttaa
    25441 aataacaaat agcaatataa taaataatgt attcaaaaat gtttcgttta gatctgttaa
    25501 aattaacaac gtaaatactc attctttaaa atttattaat tgccattttt ttaatgttga
    25561 tatgataaga gttaatttca gcaaatgtct ttttcatgac tgtagtatgc atggtgtcaa
    25621 gataaaacca tggttgccag taaaatggac aaaagagtta ataaatgatt atttgtatgg
    25681 ctgtttacta tctctgtact ctatctgtgc aagggatata tataatatga gcgccgggaa
    25741 taacgtgaag gttgctgcgg atgctttcct tgaaattatt ttctctctta agaataaata
    25801 ttgcattaaa ctattaagcg ctcaggaacg ggcgtttatt tacgaatttg caagaatgat
    25861 cttcgcttat attaatgaca aaagcattga gatcttatta ctttcttgtt ttgcggcgtc
    25921 tgaccaaaaa gcaatccaga ggtatatacc tcagtcacaa gatggggaag actttaggag
    25981 tcatttacaa tataagttgc ctctttccgc tcattaatgt tgttggtgca acataataag
    26041 acaggtaagc ggtaccctat aatgaaacca atatcgggtt atgcaacgga taatcgtaaa
    26101 gacttaagtg ggtgtttttg acacaatgag atgatttcct aaactgatca aaaccttctt
    26161 cccaacatgg catatcagtt cgataatgct ttagagatcg aaggcttaac aaacaaggag
    26221 atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg ttgaatattg
    26281 cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt tctttgccgg
    26341 tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg
    26401 ggagatcgcc tttgaggaat taggctccca ggcgcgggcg ctgggggccg atgccgtcgt
    26461 cggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga tggttagcgt
    26521 cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctggt cgctgccgct
    26581 ctgttattgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt
    26641 gatacccgac gccaggcgca ggcggcgtat ccgcgcatta aagtgctggt gatccactac
    26701 accgcagatg attttgatag ctcgctggcg acactgaccg ataagcaggt cagctcgcat
    26761 tatctggtcc ctgcggtacc accgcgatac aacggtaaac cgcgcatctg gcaactggtg
    26821 ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt
    26881 aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc ggccggagtg
    26941 aaatattttg ccccgtttga accgacacag attcaggcgc ttattccact ggcgaaagat
    27001 attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga tatcgcaccg
    27061 cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca gcaggggatt
    27121 ggtgcctggc cggatgcgca gcgggttaac ttttaccttg ccgggcgcgc gccgcacaca
    27181 ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga cgttaaacct
    27241 gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca tttccgcccg
    27301 acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc attgctggag
    27361 aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc
    27421 tcgatacctt catctagggt gatgatcggc tgataaccta actcttcctg cgcacgcgta
    27481 atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag cggcggctct
    27541 tttgctgact tgcggcctaa acgctccatg ctgcgggcga tcatatccag catcgggtag
    27601 gggacggaac gaatacgaca gtcaatattc aactcgtcga tcagcttctg cacgatgctg
    27661 cgcagtgtgc gatgctcgcc gttggtgata ttgtacacac gcccggaagg tagcttatcg
    27721 caggcttcct ggcttgccag ccacattgcg tgcacggtat tttcatagta ggtcatatcg
    27781 accagcgcac tgccgccatg cggtaacaga atactgccgt agtggtgcat catatgcgcc
    27841 agacggggaa taaagacttt atcgtgcggt ccgaacagac tttgtgggcg cagaatagta
    27901 aagcgcgttt gtggattcgc ctgcgaaagc atattgatca cttcttcgct ggctgctttg
    27961 ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat cttctttaat atcgcgatgg
    28021 tggtgataat cgaagtacag ggagggggaa gagatatgaa taaagttacg cacaccccag
    28081 gcgacagccc attcacccag gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt
    28141 tgtgtccccc agggtgaggt aaagctggag cagtgccaca gcgtatcaat gcccgcgagc
    28201 atcactttag cttgtgatga aaccagctcg gtcagatccg ccggaacaaa ctctgcgccc
    28261 attttttcca gcaatttgcc cattgcctcg ttgcgaccgg tcgctcgcac gctgatgcct
    28321 ttctggcata aaaactctac cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt
    28381 accttcatat caatccactg ttgttgagaa aatgacgtgc gcattcttcc gtgatttccc
    28441 ccatgatgca atgggaaaca tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt
    28501 gttctgccag tctggcgatt tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga
    28561 tcatcaataa ccagtaaaac agccccggca taccgtgcgg atgccagaaa gcgcggacat
    28621 cgatagtacg atagtcgcct ttatcttcca ggctaaaaca cagtcgtccc agccccgacg
    28681 ctttcatgcc aaataacaac gtaagttgtt tttccggttc aacgacaatc actttccagc
    28741 tatccaccgc atcgccagtc tgtaaatatt cgcgctccgg gcggcctttc gccagcttat
    28801 gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa aatattgcca aagaaataac
    28861 gctctttacc gccgatttgg ttcactacct gccataaagc agcaaggctg gcggacgttt
    28921 taacggtaaa ccccgcctgt ttggcaaaat aaccgtactc cggtcgccag cgggcaaagg
    28981 cctgagcgtc gtagccccag tcgctggagt tgaccagttt ttcctcctct ttcaacgtgc
    29041 tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat
    29101 catccgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg gtgggcggta
    29161 cggaagtaat cacattgaga aaccacaccg aaatccagcg ggtggggagg gggatgggga
    29221 tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc tgataactga
    29281 gcacctctgg tccggcggct tcgaagatgc ggtgttcgct ggccggatga tctaacaacg
    29341 ccaccagata gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc
    29401 gtggcggcgt taacactggc aggttgtaga ccatatcgcg catgacttcg aacgccgctg
    29461 aacctgcgcc aacgataatt ccggcccgaa gttcggtcac aggtacattc gcttcacgaa
    29521 gaatgtccgc cgtagcctga cgagcacgca gatgatccga ctgctcatgt ggcggggcct
    29581 gcaacgaact gagaaagatt aattgcttaa ctggtacttc acgtagcgca tcgcggacgt
    29641 tgagagccac ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca
    29701 gaaaatagac ggtatcgata tcctgcaaca gggccggaag gttatccggc cagctgagat
    29761 cgactttatg gcaactgacg tttgccagtt gcagctttgc aagcctgtcg acatgacgtg
    29821 ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt
    29881 agccactggc accgagaact aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg
    29941 aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc
    30001 accaggcgga caatcggcga ggcgttaatc agcacgtttc tcgctttcat gtattcgcct
    30061 aacgcggcag cattttcttc cccgacgcga acaaacagca tattagtgtc ctgacgcatc
    30121 acatccgcgc ctgcttcacg cagttgctcc gccatccagg cggcgttgtc gtgatcttcc
    30181 tgcaaccgtg cgacgttatt tttcagggca tacatcccgg cggcagccag aatcccggac
    30241 tgacgcatcc cgccaccggc cattttccgc cagcggatgg cacgtttaat gtaatcacga
    30301 ttaccgacga gtaatgaacc gactggcgtc ccaagacctt tcgacaggca aatggtgaac
    30361 gaatcacaat attgcgtgat ctctttcagt tcgcagccgt aagccaccac ggcattaaag
    30421 atgcgcgcac cgtcaacatg cagcgccaga ttgcgcttgc gggtaaattc ccatgcttct
    30481 ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt tttctagact gagtaatttg
    30541 gtgcgggcga aatggatatc gtcgggtttg attttcatcg ccactttatc cagcggtagc
    30601 gtgccgtcgg cagccgcgtc gatgggttgc ggttgaatac tgcccagcac cgctgcgcca
    30661 ccggcttcaa acagataatt atgcgcggcc tgaccgacaa tatactcttc gccgcgttcg
    30721 cagtgactga gcagagcgac caggttagcc tgggtgccgg tcggcagaaa aatggcggct
    30781 tctttaccgg aaagctctgc ggcgtagtcc tgcagagcat taacggtagg gtcgtctccg
    30841 taaacgtcgt ccccaaccgg ggcggccatc attgcttcga gcatggcgcg gctcggtcgg
    30901 gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt attatgacgg gaaatgccac
    30961 cctttttacc ttagccagtt cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg
    31021 atgattgcgc gcaacatata caagctaaat cctttggcct gttcgagttt gatctgcggt
    31081 ggaatggcta actcttcttt ggcgaccacc acatccacca gcaccggacc gtcgatggag
    31141 aaggcgcgtt gcagggcttc atcaacttca gacgcttttt ctacacggat acccgtaatg
    31201 ccgcacgctt cggcaatgcg ggcaaagttt gtgtcgtgta gttcggtgcc gtcagtcaaa
    31261 tagccaccag ctttcatctc catcgccaca aagcccagca cgctgttgtt aaagacgaca
    31321 attttcactg gcagtttcat ctgcactact gagaggaaat cgcccatcaa catgctaaaa
    31381 ccgccatcgc cgcacatggc gaccacctga cgttctggct ctgtcgcctg cgcacccagc
    31441 gcctgcggca tggcgttagc catcgaaccg tggttaaacg aacctaacag gcgacgcttg
    31501 ccgttcattt ttagataacg tgccgcccac accgttggcg taccaacgtc acaggtgaaa
    31561 atagcgtcat cggcggcaaa atgactaatt tgctgcgcca gatattgcgg gtgaatggct
    31621 ttctcgctcg gtttagctaa atcgtccagc cctttgcggg cgtcgcggta atcttccagc
    31681 gctttatcca gaaacttgcg atcggctttt tcttccacca atggaagcaa tgcacgcaga
    31741 gtcgacttga tatcgccgac cagtgccata tccaccttgc tgtgagcgcc gatgctggct
    31801 gggttgatat caatctgaat gattttggca tcggtcgggt agaaggcgcg gtagggaaat
    31861 tgcgtgccga gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaag
    31921 ccgattaacc cggtcattcc aacatcatac ggattatcgt attcgacatg ttctttaccg
    31981 cgcagggcat gaacaatagg cgctttaatt ttcccggcaa actcaactaa ctctttatgc
    32041 gcccccgcgc agccgctgcc acacatcagg gcgatattgc tggaataacg cagcagttgc
    32101 gccagtttgc gtaactcttc ttcttccggc gtcacgactg gttgtggcgc atgataccag
    32161 tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt cgcctggtaa cacgacaacc
    32221 gaaacgccac ggttaagcac cgctttgcgc atggcaatcg ccagtacttg tgggatctgc
    32281 tccgggctgg aaaccagctc gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg
    32341 gtttcctgga aatagccgct gccaatttcg ctggagggaa tatgagcggc aatcgccagt
    32401 accggaacgt gattgcggtg gcaatcgaac aggccgttga ttaagtgcag gttgccgggg
    32461 ccgcacgatc cggcgcagac cgccagttct ccgctaagtt gtgcttcagc gccagcggca
    32521 aaggccgcca cttcttcgtg gcgggtggac atccactcga tggtgcccat gcgattaaga
    32581 ctgtcactaa gaccgttcag agagtcgcct gtgactcccc agatgcgttt cacccctgcc
    32641 gattcgagtg ttttggcgat ataagctgca accgtttgtt tcatggttct ccatctcctg
    32701 aatgtgataa cggtaacaag tttagttcat ctgacggagg gggaagggat gggagagaaa
    32761 ggaggcacta acggttaaat agcccgatga aaggaatatc atcgggcata aggcgattat
    32821 gcgagaacca aatccccctg cggatggcag gagcaggcca gtacgtaacc ttcagcgatt
    32881 tcggcgtcgg tcagcgtcat tgtactgctc accgtatatt caccggaaac cacttttgtc
    32941 ttacagcagc cgcaaacacc cgcacggcag gcagcgacaa ccggaacgtt attgctttcc
    33001 agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg
    33061 gtgaatttca gaccgctggt cgccgcttct gctactggag tgaagaattt ctctttaaag
    33121 aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc aatccatata cggagccggg
    33181 ccgcaggtca tcacggtacg tgaagctaag tcaggtacac ctgccagcag ttcgcgagtg
    33241 agacgaccag cgataaagcc ttcggtaacg ttattttctg ccaccagcgt taccggatag
    33301 ttacgccact catcggcgaa aataacatcc tgcggcgtac gcacgttgta gatcacccgc
    33361 acatcggcct gtggacggtt cttcgcaagc cagcgacgca tcgacataat cggcgtgacg
    33421 ccgcagcctg ccgccagcaa caggaattta tcttctgctt tatcgtcgca ggtaaattcc
    33481 cccatcgcgt ccgaaagcca gagataatca ccgcgtttta catcgcgcgt cagccactgg
    33541 gagccgacac cgtcatcaat ccgccgcacg gtcagggtga tatattcact cacgcctggc
    33601 gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca
    33661 tattgcccgg cgcgatatgg gtagtaatcg tggcaaatca gggaaatcgt ccacacatcc
    33721 ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg ggcattgatt cgttggcatc
    33781 gtcatcgaca aactccttac gcgctcaaca gttgcttcat gtcttcttca acagtggtga
    33841 tagaacgcag gccgaatttc tcgttcagca ccgccagcag gtctggtgtc aggaaaccag
    33901 gtgcagtcgg gccggtgacg atatttttca cgcccagaga aagcagcgtc agcagaatga
    33961 cgatcgcttt ctgttcaaac caggagagca ccagcgacag cggcagatcg ttcacaccac
    34021 agcccagttt ctctgccaga gtgacagcca gaataatcgc tgagtaagca tcgttacatt
    34081 gacctgcatc taccagacgc ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc
    34141 gatatttacc acaggcgagg gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat
    34201 cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac
    34261 gcagtttttc acggctcacc agatcaatca gcgtatcagc agcgccaagc agcgtctggc
    34321 gaccaaaacc cacggtgata aggtgcggaa tttcgctgta cgggaagcct gccatctgtt
    34381 gcgcctgggt gataaccgca gagaaatcat caccgtccag atgacgcacg ccaggccagc
    34441 caacaatgct gcgggtccag atacgatcgt cataagcgcc tacggttggg tcgatgatgc
    34501 agttcgaggt catcacgatg gggccaggga aacgagcgaa ctccacttgc tgattctgcc
    34561 agccgctgcc gtagttaccg accagatgct tgaatttacg cagctccggg tagccatgcg
    34621 caggcagcat ttcgccgtgg gtgtagacat taacgcccgt gccttcggtc tgttccagca
    34681 ggttgtagag atctttgaga tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt
    34741 tgacgttgac ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa
    34801 tgctcatcac tttgaagttc atctggccga tttccattga acactcaaga agcgcgttca
    34861 tatcggcagg ccaggtcccc agccacgcca tgattttatg gtactgggca taaatatcgt
    34921 tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat
    34981 acaggcacag cagacgcagg ccgagaatgt tttcgccaat cgccgcttta tctttgttag
    35041 gggtaaattc tgctgcctga cgttgcagct cgccgagatc atcgctcacc agttgcaggt
    35101 cagccatcgg gttatcgacg cgcgcgttgg catctacagc caggcattgt gctttcagcg
    35161 cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat acgcggagaa tcgaagttaa
    35221 cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga
    35281 tgccgtattc acacgctttt accgcccagg cagaaagccc ttgcagcgcc gcgatgagta
    35341 aatcctgaag gtcagaagtt tccgccgttt taccacacat cccctgcgcg tatgagcagc
    35401 cgtttcctgc cggagtacgg atagtttgtt cacattgcac acaaaacatg atcacacctt
    35461 ttaaagttat atttaatata catgtttaag gttaagacgc ttaacgcggg gataaaaggg
    35521 atttttcatg caactttaag ggagattgat ttagcgcaat tttggcggca gggatctacc
    35581 gccagagagg tattacgcag agaaaaaggc gatgaggatc ggcactaaca ggctaagaat
    35641 aaaaccgtga acaattgccg ccgggaccat atccagcccg ccagtacgtt gaagaacggg
    35701 cagggtgaaa tccattgatg tggcaccgca taagcccagt gcagtagagc ggctgcggcg
    35761 aatcagccca gggatcaaca taatagcaat cagttcacgg gccagatcat taaaaaacgc
    35821 cgcgctcccg attaccggac caaaagattc ggtcaataaa ataccggaaa gagaatacca
    35881 gccgaaaccg gaggccattg ccagcgcggt attgatgggg agatcaagaa taaaggcgtt
    35941 aattaaacca ccaattaatg aactgacaac caccaccacg gcgacaatca ttccccggcg
    36001 attaaggaca atctgcttta aggtcatgcc attattgcgc aactgaatac caacgaggaa
    36061 aagtagcaaa attaacgtgt attcactggc ttcggtcgcg tgttgtaaga aagccagtcc
    36121 actcagacca atggcaaaac caatcactac tacgccgcac agttttagcg actccagcgc
    36181 catcgcaata cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg
    36241 ctccagccac atcagggcgg caatattaca cagtaaaata acggtaatac tgacggcaga
    36301 ataatgcaga atcgccaaca ggttactggc gaggttatcg agaaacgcca gactgatacc
    36361 cataaaaaag agaataaggt aaaccatcca gcttaatagc tgattaataa cttttaacgc
    36421 agcttgttgg cgaagcggaa tgaggtaacc tacaatcagg ggaaccagaa tgattaacag
    36481 cccagaaaac atgaaaaccc agtccttgca aagatgaagt cgaaatgcgc gatgacacac
    36541 tactgaaagc ggaaggacga gtaaagttgc aattaaaagg aaatgttatg cataaggagc
    36601 agtagagtat tcgttttcat ttaaagatat tcttgcgctt taattacaaa ctgcaccgat
    36661 gttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc acatggatgt
    36721 gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccggtag ccgtaaggga
    36781 taagtcgagg gcaccgcgca gcggcgattt tgttcgccag agcccggggg ggcagggggc
    36841 ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagctc
    36901 atgaaaggag cgcaacaaga tgatgaatca acatataaca acatcttaaa aaaaggcctg
    36961 acattacgcc aggccttctg cgttaattaa tcacgctttt ccagcagggt ccggtaaatc
    37021 agaccaccga taatgccgcc gacaattggc accacccaga agaaccacag ttgttctaat
    37081 gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta
    37141 gtcaccggaa tactaattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca
    37201 aaacctgccg gcgcgaattt gtcggttgcg ccgtggatca ccaacaggaa acctgcactc
    37261 aataccagtt caactaccag cg
//
LOCUS       DAJTXQ010000029        37180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000029 DAJTXQ010000000
VERSION     DAJTXQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.029"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..487)
                     /locus_tag="OO515_001371"
     CDS             complement(<1..487)
                     /locus_tag="OO515_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6575339.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NP"
     gene            complement(665..1576)
                     /gene="gsiD"
                     /locus_tag="OO515_001372"
     CDS             complement(665..1576)
                     /gene="gsiD"
                     /locus_tag="OO515_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6575340.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1579..2499)
                     /gene="gsiC"
                     /locus_tag="OO515_001373"
     CDS             complement(1579..2499)
                     /gene="gsiC"
                     /locus_tag="OO515_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6575341.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2517..4055)
                     /gene="gsiB"
                     /locus_tag="OO515_001374"
     CDS             complement(2517..4055)
                     /gene="gsiB"
                     /locus_tag="OO515_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6575342.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4075..5946)
                     /gene="gsiA"
                     /locus_tag="OO515_001375"
     CDS             complement(4075..5946)
                     /gene="gsiA"
                     /locus_tag="OO515_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6575343.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5933..6898)
                     /gene="iaaA"
                     /locus_tag="OO515_001376"
     CDS             complement(5933..6898)
                     /gene="iaaA"
                     /locus_tag="OO515_001376"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6575344.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7102..8337
                     /gene="moeA"
                     /locus_tag="OO515_001377"
     CDS             7102..8337
                     /gene="moeA"
                     /locus_tag="OO515_001377"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6575345.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8337..9086
                     /gene="moeB"
                     /locus_tag="OO515_001378"
     CDS             8337..9086
                     /gene="moeB"
                     /locus_tag="OO515_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6575346.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9265..9927)
                     /gene="fsa"
                     /locus_tag="OO515_001379"
     CDS             complement(9265..9927)
                     /gene="fsa"
                     /locus_tag="OO515_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6575347.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10058..10957
                     /locus_tag="OO515_001380"
     CDS             10058..10957
                     /locus_tag="OO515_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6575348.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10963..13395
                     /locus_tag="OO515_001381"
     CDS             10963..13395
                     /locus_tag="OO515_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6575349.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13541..14356
                     /gene="ybiV"
                     /locus_tag="OO515_001382"
     CDS             13541..14356
                     /gene="ybiV"
                     /locus_tag="OO515_001382"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6575350.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14508..15773
                     /locus_tag="OO515_001383"
     CDS             14508..15773
                     /locus_tag="OO515_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6575351.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15740..16900)
                     /locus_tag="OO515_001384"
     CDS             complement(15740..16900)
                     /locus_tag="OO515_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6575352.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17138..18439
                     /locus_tag="OO515_001385"
     CDS             17138..18439
                     /locus_tag="OO515_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575353.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18454..20817
                     /locus_tag="OO515_001386"
     CDS             18454..20817
                     /locus_tag="OO515_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCR6575354.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20997..22592)
                     /locus_tag="OO515_001387"
     CDS             complement(20997..22592)
                     /locus_tag="OO515_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6575355.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22811..23731
                     /gene="ldtB"
                     /locus_tag="OO515_001388"
     CDS             22811..23731
                     /gene="ldtB"
                     /locus_tag="OO515_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6575356.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23790..24908)
                     /locus_tag="OO515_001389"
     CDS             complement(23790..24908)
                     /locus_tag="OO515_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6575357.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24905..25372)
                     /gene="mntR"
                     /locus_tag="OO515_001390"
     CDS             complement(24905..25372)
                     /gene="mntR"
                     /locus_tag="OO515_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6575358.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25558..25686
                     /gene="mntS"
                     /locus_tag="OO515_001391"
     CDS             25558..25686
                     /gene="mntS"
                     /locus_tag="OO515_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6575359.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25958..27541
                     /gene="opgE"
                     /locus_tag="OO515_001392"
     CDS             25958..27541
                     /gene="opgE"
                     /locus_tag="OO515_001392"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6575360.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27590..28105)
                     /gene="ompX"
                     /locus_tag="OO515_001393"
     CDS             complement(27590..28105)
                     /gene="ompX"
                     /locus_tag="OO515_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6575361.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28158..28223)
                     /locus_tag="OO515_001394"
     CDS             complement(28158..28223)
                     /locus_tag="OO515_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575362.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28458..29345
                     /gene="rhtA"
                     /locus_tag="OO515_001395"
     CDS             28458..29345
                     /gene="rhtA"
                     /locus_tag="OO515_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6575363.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29644..30147
                     /gene="dps"
                     /locus_tag="OO515_001396"
                     /gene_synonym="pexB"
     CDS             29644..30147
                     /gene="dps"
                     /locus_tag="OO515_001396"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6575364.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30551..31297
                     /gene="glnH"
                     /locus_tag="OO515_001397"
     CDS             30551..31297
                     /gene="glnH"
                     /locus_tag="OO515_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6575365.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31436..32095
                     /gene="glnP"
                     /locus_tag="OO515_001398"
     CDS             31436..32095
                     /gene="glnP"
                     /locus_tag="OO515_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6575366.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32092..32814
                     /gene="glnQ"
                     /locus_tag="OO515_001399"
     CDS             32092..32814
                     /gene="glnQ"
                     /locus_tag="OO515_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6575367.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32931..35156
                     /gene="ybiO"
                     /locus_tag="OO515_001400"
     CDS             32931..35156
                     /gene="ybiO"
                     /locus_tag="OO515_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6575368.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35153..36079)
                     /gene="rlmF"
                     /locus_tag="OO515_001401"
     CDS             complement(35153..36079)
                     /gene="rlmF"
                     /locus_tag="OO515_001401"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6575369.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36355..36615
                     /locus_tag="OO515_001402"
     CDS             36355..36615
                     /locus_tag="OO515_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6575370.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36880..>37180
                     /locus_tag="OO515_001403"
     CDS             36880..>37180
                     /locus_tag="OO515_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6575371.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9008 a        10045 c         9323 g         8804 t
ORIGIN      
        1 ccggattcca gctgttgtta gagtcatctg aagccaccga ccacggtcgt ttcgatgggt
       61 taaaatcggc aaaaataacc cgccctgact cgtcgacaag cggtccattt acttgttcct
      121 gatgataaac aacgatttta tcattcgccc tccgcgaaaa gagataatat ttattatcat
      181 caagtaccag tcccaccgag tctaaaaatg gcatcatttc cattttcaga atcacggcgc
      241 gttgaatctc ctctgtattt tttaataaat acagtgagtc ctttaatgtc agggccgaac
      301 gttcaatatc tttaaagaaa tagccaacgt ttttctgaat aagttcaaag cggctatcgg
      361 cacgttttac cgcgcgttcc gataccagtt gatagttaat gatgaaaaaa agaccaataa
      421 atgacagtgc tgccaataaa aataaaatta tcagccttat ctttatcttt tcgtataaac
      481 tcagcatgtt aatacattta acaaaataac tatctgaacg ctgtgccgct gcaaaaatag
      541 tcccttgctg cggcttttta accaaattac ccgattctac aaaatcagca gaaagttaca
      601 acacttgctt agggcaataa tttcctgtga ggtgattacc ctttcaagca atattcaaac
      661 gtaactatcc tttaattttc ggatccagcg catcgcgtaa accatcgccc aacaaattga
      721 acgccagtac ggtcagaaaa atagccaggg ccggaaaaac agcgacatgc ggcgcgataa
      781 ccatatccgc tcgtgcctca ttgagcattg ctccccactc tggtgtcggc ggctgcgcac
      841 cgaggccgag aaatgacagg ctggcggcag agataatcga ggtgccaatg cgcatggtga
      901 aaaacaccac gatagacgag acggtcccag gcaagatatg acgcaataaa atggtcatat
      961 cgctggcacc aatactgcgt gctgactcaa taaaggtttg ctgtttcaac accagcgtgt
     1021 tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt
     1081 tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc cagtaaaata cccgggaagg
     1141 caaacagcac atcgcaaatg cgcatgatca gccgatccca ccagccttca taatatccag
     1201 ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa cacggcaaac acgcccgccg
     1261 ccagcgagat ttgcgcacca accaggacac ggctgaaaat gtcgcgcccc agtgaatcga
     1321 cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt gtcataatca aaataatttt
     1381 cggcgtcata gggagcgatc cagcgtgcaa aaatggccac cacaatcaat aaaataacga
     1441 ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg tcgccagaat tcatgccacg
     1501 gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc
     1561 agttaaatag tcgcatcctt acttgtacct gatagccggg ttaatggcgg cgtaaagcac
     1621 atccaccact aagttgataa gaataaattc cagcgagaaa agcagaattt ccgcctgaat
     1681 caccgggtaa tcgcgcattt ctacggagtc aacgagtaag cgtccaagtc cgggccagtt
     1741 gaaaactttc tccaccacga tagaaccacc gagcaaaaag ccaaactgta agcccatcat
     1801 ggtcactacc gggatcatcg cgttacgtag cccgtgtttg aggacaaccc aggtttcgct
     1861 cacccctttc gccctcgcgg tacgcatata atcttcgctt aaaacatcga caaacgacgc
     1921 gcgggtaaag cgcgccatca cggctgccac tgccgcgccg agcgtcaggg agggtaaaat
     1981 gtagtgctgc caactgtctg ctcccacggt aggcagccag cccagttcaa cggagaatac
     2041 ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag atccccgaca ccgcaatggt
     2101 catactcaat cgatccggcc aacgattacg ccagacggcg gcgataattc ccgccgccat
     2161 accaaatatc accgcccaga ccatactggt tatggtcagc cacagcgttg gcataaagcg
     2221 gctggcaatc tcatcggcaa ccggacgacg cgacaccatc gacaggccaa aatccccctg
     2281 cacagcattg ctgatatagt gccagaactg gtgatacagc ggctgatcca accccagctg
     2341 ctgacgcacc agttctataa cctgcgcatc agcttcgagc ccggcaatca atcgcgccgg
     2401 atcgccgggc agcatatgca caaataaaaa caccagcacc gagacgataa acagcgtcgg
     2461 aatcaacccc agtaagcgtt tgataacgta attaagcatt ccactccctg cgttgcttat
     2521 tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca tgatccaaaa accggtcagg
     2581 tttttactgt gtgccgacac cagtttttct accaccagcg ggatccacgg cgactcttgc
     2641 cagatgatat cctgcgccgc cttatacaag cgggtctttt ccgccggatc attagttttc
     2701 agtgcctgag ccaggaagtc gtccacctgt ttattgctgt aaaatgcggt attaaacagc
     2761 gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg cccagtccgc ttcgccggtt
     2821 gaagccgacc agccagtgta gaacatccgc acgccgctct ctttttgccc tttaccttca
     2881 acttctgccg cccgctgtcc ggcatccatc gcggtcacct gggctttaat cccgacctgc
     2941 gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac
     3001 cacagcgtgg tactgaaacc gttgggatat cccgcctctt tcagtaattc gcgcgctttc
     3061 actggatcgt aaggccacgg tttataactt tgcgcgtagg cgatacttgg cggtaccaca
     3121 ccagtagctg gcgttgcata gcccgcaaag gcaactttca ccagcgccgg acggttaatg
     3181 gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg gcttttgcgt cacgttcata
     3241 ctgatataac gctgcataat tgacggactg gccatcaact cgatattttt gtttttctcc
     3301 agcagtgtgg cctgctcgta gggaatgggg aaagcaaact gcgcttcacc ggtttgcagc
     3361 attgccgcgc gggtgttgtt atccgccacc ggacgccagg ttatgctgtc cagtttgggc
     3421 aatcctggct gccagtaacc cgcgaatttt ttcaccttca caaaatcggt ctgattccag
     3481 gtatccagtt catacggtcc ggttcccacc ggatgaaaac caatctcctt gccatatttt
     3541 tccagcgctg ccggtgaaat catcgcggtc gccggatggg caagaatatt aataaacgct
     3601 gagaacggct gtttgagggt aatctttacc gttgtcggat cgatcgcctc cgttttggca
     3661 atattcttat acaggttata gcgtttaaga tgattcgccg gatcgctggc ccggtccaga
     3721 ttcgctttta ccgccactgc gttgaaatcg gtgccatcct ggaatttaat gccttcccgc
     3781 aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat aactctccgc cagcacgttt
     3841 ttcagtttca tctctttatc cagaccgaac agcccctggt aaaacgattt cgctacggcc
     3901 tgagataacg tatcattcgc gtcatacgga tcgagcgtgg tgaaattcga tcctaccgcc
     3961 accaccacat ctttggcagc gaatgcacaa gatgccatca acgctgtcgc aatgcccagc
     4021 gccactaacc cactacggtg tacagctctt gccattttat tctccgcctg aatgttatct
     4081 acgcatgaat gcgtattctg attgtggttg tgcgacgtaa tgccccggcc cgacgcattg
     4141 caacgagacg gctgccacct cttcgccacg cagatgaata ttgctgggaa gatcgtccga
     4201 cagcagtaca cgctgcggtc gttgtcggga cggttcagcg accggaactg ccgccagtaa
     4261 tttacgcgta taaggatgct gcgggttttc gaagaccgcg cgccgtggac caatttcaac
     4321 aatttgcccg agatacatca ccgccacacg atgactaatc cgctctacca cggccatatc
     4381 gtgggagata aacagatacg caatgccgaa atcacgctgg agatcgagca acaagttgat
     4441 aatctgcccg cgaatagaaa catccagcgc cgaaacggct tcgtcggcaa tgatcacttt
     4501 tggattcaat gccaacgcgc gagcaatgca gatgcgctgg cgctgaccgc cggaaaactc
     4561 atgcgggtaa cgccaggcat gttcaggtaa caggcccacg cgctccagca accacgcaac
     4621 gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca cgcagcggtt cgataatcga
     4681 atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg tcctgaaaaa taaactgaat
     4741 atcccggcgc aatgcctgaa gtttgccggg tgacaaggta tcgattctct gaccgttaaa
     4801 gataatttcg ccgccctgcg attcgaccag gcgcagcaac gcccgcccgg tagtggattt
     4861 accgctgcca gactcgccca ccagcgatag cgtttcgcca ggccagagat caaaactgac
     4921 tttctcaacg gcatgcactt cccgcgttac gcgattcaac aaaccgctgc gcaaagggaa
     4981 acgggtgaca agattacgca ctcgtaaaac aggttcgcca tccaccaccg ttttctgctc
     5041 gatggggggg gcctgtttcg ctggatgttc aagcgatatc aacgggaaac gtcggggata
     5101 atccaaccct ttcatcgcac caagttgcgg aacagcagct aacagcgcac gggtgtaagg
     5161 atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt tccaccgcct cgccctgata
     5221 catcaccagt acccgatcgg caatctctgc caccacgccc atatcgtgag tgataaagat
     5281 aacgcccatc gacatctctt tttgcaatac tttgattaat tgcaggatct gcgcctgaat
     5341 agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc accgccgggc ggcatgacag
     5401 cgccatcgca atcatcactc gctggcgcat cccgccagag agttgatgcg gataacgtga
     5461 aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc atccgcttcg cctcgaccat
     5521 cgcttcttca cgactggcgt tctgatgcag acgaattgat tcggcaatct gttcacccac
     5581 agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat atcatcgcca tatccgcacc
     5641 gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt tcaatcactt cgcgactgcg
     5701 ccgctgcaac agcattttat cgcactgtac taaaccgccc gcctgttcca acaggcgcat
     5761 caatgccaac gcagtcactg acttaccgga gccggattcg ccaacaattg ccagcgtctc
     5821 accgcgttgc agactaaaag agagattgcg gaccgcagct attttctgct ggtcctgcat
     5881 aaaggcaata ttcagatttt caaccgccag cacattaccg gcatcaagtt catcactgtg
     5941 tggcaacggt gtcccctttt tcacggtaga taccggtggt tggcgtatcg cctgcgtagc
     6001 cccaggcgcg atacattcct tcggtgttaa acggtagcgc gacattccct tcatggtcga
     6061 tagcgattaa gccaccgcta ccgccaagcg cagggagttt ttccattact acccgctcgc
     6121 aggcttccgc gagacttaat ccgccgtaat ccattaacgc ggcgatgtca tatgccgcca
     6181 gcgcgcggat gaagacttcg cccgtgccga tacaagaaac cgccacactg gcgttattgg
     6241 cgtagcatcc ggcacccact aagggactat cgccaactcg tccgggtaat ttattggtca
     6301 ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa atccaacgcc acggccccca
     6361 cggtgcccat tttctgtttt tcatccagtg gcgcaccgct atggtcgagg acggttgccc
     6421 cttccttgcg cgctgccagt agttgttcat aacgcaaagg cgtggagaaa atctccggcg
     6481 agacgcgctc catgccacga gcaaacgcaa aattttctgc cccttcgcca atcatcatca
     6541 catgcgggct ttgctccatc accagccggg cggcaagaac cggattacgc agatgactaa
     6601 cgcccgccac cgcaccggct ttcagggtgt taccatccat cacacaggcg tccagttcat
     6661 gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt aaacagtgga cactcttcca
     6721 gcagacgcac cgcttccgtc accacatcca gcgcactttc gcccgcttcc agcattttct
     6781 gcccggtttc aacaatggca gacaacgcct caatgtagcg taattcctgt tgcagactca
     6841 tctgcgcgcg gctaattgca cctgcgccac catgaattgc aatgactgct ttgcccatag
     6901 tattcgtcca ttaagggtgg ctcgccactt ttgctataaa tatcagaatt tttatgaatt
     6961 actcattcaa tttttgaata tagaaagatg gatatggcat gtaaaggcag gagccaacgc
     7021 tacgcccgag gtggcataca gtctgtggcg tttttctgac ataataggca aattcgattt
     7081 tgcctccgca ggagtgtttt catggaattt accaccggat tgatgtcgct cgacaccgcg
     7141 cttaatgaga tgctttctcg cgtcacccca ctgaccgccc aggaaacgct gccactggta
     7201 cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc cgcttgatgt tccggggttt
     7261 gataactccg caatggacgg ctacgcggtg cgtttagccg atattgcctc cgggcaaccg
     7321 ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc atggtgaatg gcctgcgggc
     7381 acctgcattc gtattatgac cggtgcgccg gtgccggaag gctgcgaagc ggtggtgatg
     7441 caggagcaga ctgaacaaac ggacaatggc gtgcgtttta ctgctgaagt gcgtagcggg
     7501 caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg cggttgtttt cccggcggga
     7561 actcgcctga ctaccgcaga gctgccagta attgcttcac tggggattgc cgaagttccg
     7621 gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg atgaactcca gttgcccggt
     7681 cagccgctgg gcgacggcca aatctacgac accaaccgtc tcgccgtaca cctgatgtta
     7741 gaacagctgg gttgcgaggt aatcaactta gggattatcc gcgacgatcc ccatgccctg
     7801 cgcgccgcat ttattgaagc cgacagccag gcggatgtag tgatcagttc cggcggtgtt
     7861 tcagtgggtg aagcggatta caccaaaacg attcttgaag agctggggga gatcgccttc
     7921 tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg gtaaactcag caatagctgg
     7981 ttctgcggcc tgccgggcaa cccggtttca gcgacgctga ccttctatca actggtacag
     8041 cctttgctgg caaaactaag cggcaacacc gccagcggcc tgcccgcgcg ccagcgcgta
     8101 cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg atttccagcg cggcgtgctg
     8161 caacgcaacg ccgacggtga acttgaagtg acgaccaccg gacatcaggg ttcacatata
     8221 tttagctcct ttagtctcgg caactgcttt atcgtgctgg aacgcgatcg cggcaatgtg
     8281 gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt tgttcggagg cctgtaatgg
     8341 cggaactcag cgatcaggag atgctgcgct acaaccggca aatcattctg cgcggttttg
     8401 attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct gatcgtcggc ctcggtggac
     8461 tcggctgcgc agcttcgctg tatctggcaa gcgccggtgt cggcaacctg acgctgctcg
     8521 acttcgacac ggtttcgctc tcgaatctgc aacgccagac actgcacagt gatgccacgg
     8581 tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac gcggatcaac ccacatatcg
     8641 cgattacgcc agtcaatgca ctgctagatg acgcagaact tgcagcattg attgctgaac
     8701 acgatctggt gctcgactgt acggataacg ttgcggtacg taatcaactg aacgcaggct
     8761 gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat tcgtatggaa gggcaaatca
     8821 cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg ccttagccgt ttgtttggcg
     8881 agaacgcatt aacctgcgta gaagcaggcg tgatggcacc gttgatcggc gtaattggtt
     8941 cgttgcaggc aatggaagcg atcaaactgc tggcaggtta tggcaaaccg accagcggga
     9001 aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga aatgaaactg atgcgcaatc
     9061 cggggtgtga ggtgtgcggg cagtaattgc cacctccagg cctgataaga cgcgacaagc
     9121 gtcgcatcag gcatttgctc cagacgccag atgcggcgtg aacgccttat ctggcctacg
     9181 gttcgggcac agatttccaa gcgcggcagg catcggtgca ctccgccacg tcatcagcaa
     9241 tttatgagga tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc cctgccagtc
     9301 ctgctcaaac ttcgccacag cggcttccac cgccggatag ctaatcatct gttgtgccac
     9361 atccagtggc agagtaattg attcacatcc tgccagtaag cagtccagcg cctgacgcgg
     9421 ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca tgcattttca ataactggtg
     9481 taagtcggtc acagtctgaa tgccgctacc gccctgagca tcaatacgat taacgtaagg
     9541 cgcaacatat tccgcacctg ccagcgccga cagcaaccct tgtgctgcgc catataccgc
     9601 ggttcccagc gttggaatcc cttccgcttt taacatctta atagctgcca gcccctcggc
     9661 ggtcaccgga actttcacca cgatatccgc aataatagaa cgcaatttac gtgcgtcatt
     9721 caccatccct tcagcagtgg tagccattac ctgggcaaac agacgcccct gaccgcccat
     9781 cgcttcatga agttgcggaa gcacaacatc cagcggtttt ttacccgcgg cgataatgct
     9841 tgggttagtg gtcacacccg ccagcggaaa aatacgtgac agcgccttca ccgcaacaac
     9901 gtctgaagta tccagataca gttccataac catcctcaaa atgtactttt tgtctgaatg
     9961 tagactatca cgggaaaact ctctgaacag ttgacgcaca tcaatctaac tttcattcga
    10021 aagtatttta atgtttgaac gaaacagaga ggcgactatg attttcaata ttcagcgcta
    10081 ctcgacccat gatggccccg gtatccgcac ggtcgtattt cttaaaggct gttcgctggg
    10141 ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg caggatctgc tgtatgacgc
    10201 acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag tgattgagcg
    10261 cgcgctgaat ggtttgctta ttcatcggga aaagttaacc ccggagcatc tgacggcgtt
    10321 aaccgactgc tgtccgacac aggcattaac cgtgtgtggt gaagtgaaaa gcgttgagga
    10381 gatcatgacg accgttctgc gcgataaacc gttttacgat cgcagcggcg gcggtttaac
    10441 gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg atggcgctac tgcaagccag
    10501 ccacgaggca ggcattcata ctgcggtaga aacctgtctg catgtgccgt ggaaatatat
    10561 cgccccttct ctgccctata tcgatctgtt tcttgccgat ttaaaacacg ttgccgacgc
    10621 gccgtttaaa cagtggaccg acggtaacgc cgccagagtg ctggataacc tgaaaaaact
    10681 cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg attcagggct ttaatgccga
    10741 cgaaacctct gtaaaagcta ttaccgattt tgccgccgac gagctgcacg ttggcgaaat
    10801 tcatttcctg ccctaccaca cgctaggcat caacaaatat cacttactta atctgcccta
    10861 tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc gactttgccc agcagtatgc
    10921 ctgccagaaa gggttaaccg cgaccttacg aggataacaa ccatgaccac actgaaactg
    10981 gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat tgtgaaaccg
    11041 ccagtctgta ccgagcgcgc gcagcactat accgagatgt atcaacaaca tctcgataag
    11101 ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc tggcgaatcg caccatctgg
    11161 atcaaacacg atgagttgat cattggcaac caggcaagcg aagttcgcgc cgcgccgatc
    11221 ttcccggaat atactgtctc gtggatcgaa aaagagattg atgatctggc agatcgtccc
    11281 ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc tgcatgaagt gtgcccgtgg
    11341 tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt ttaccgatga gcaaaaaggt
    11401 ctgctggcga ccggaatcat taaagcggaa ggcaatatga cctccggcga tgcgcacctg
    11461 gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg gtctgcgcga gaaagtagcg
    11521 gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt tgcacggcga acagttcctg
    11581 aaagcgattg atatcgtgct ggtggcagtc agcgaacaca ttgaacgttt tgcagccctg
    11641 gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc gcgatgaact gctggcgata
    11701 gcagaaaact gcgatcttat cgcccaccag ccgccgcaga ctttctggca ggcgctgcaa
    11761 ctgtgttact tcatccaatt gatattgcag atcgaatcta acggtcactc ggtatcgttt
    11821 ggtcgtatgg accagtatct ctacccgtac tatcgccgcg acgttgaact caaccagaca
    11881 ctggatcgcg aacacgccat cgaattgctg catagctgct ggctgaaact gctggaagtg
    11941 aacaagatcc gctccggctc acactcaaaa gcctctgcgg gaagtcctct gtatcagaac
    12001 gtcaccattg gcggacaaac tctggttgat ggtcaaccaa tggacgcggt gaatccactc
    12061 tcttacgcta tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa cctcagcgtg
    12121 cgttaccatg caggaatgag taatgatttc ctcgacgcct gcgtacaggt gatccgttgc
    12181 ggcttcggga tgccggcgtt caacaacgac gaaatcgtga tcccggaatt tattaaactc
    12241 ggtattgaac cgcaggacgc ttacgactac gcagcgattg gttgtatcga aaccgccgtc
    12301 ggtggcaaat ggggctatcg ctgtaccggc atgagcttta tcaacttcgc ccgcgtgatg
    12361 ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca aagtgttcct gccacaagaa
    12421 aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag taatggacgc gtgggatacg
    12481 caaatccgtt attacacgcg taaatcaatc gaaatcgaat atgtcgtcga caccatgctg
    12541 gaagagaacg tgcacgatat tctctgctcg gcgctggtgg atgactgtat tgagcgagcg
    12601 aaaagtatca agcaaggcgg cgcgaaatat gactgggttt ctggcctgca ggttggcatt
    12661 gccaacctcg gcaacagcct ggcggcagtg aagaaactgg tatttgaaca gggcgcgatt
    12721 ggtcagcaac agctggctgc cgcactggcg gatgacttcg atggcctgac tcatgagcaa
    12781 ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca acgacgatga taccgtcgat
    12841 acgctgctgg ctcgcgctta tcagacctat atcgacgaac tgaaacagta ccataacccg
    12901 cgctacggtc gtggtccggt tggcggcaac tattacgcgg gtacctcgtc tatctccgct
    12961 aacgtaccgt ttggcgcgca aactatggca acaccggacg ggcgtaaagc ccacaccccg
    13021 ctggcagaag gcgcaagccc ggcctccggt actgaccatc ttggccctac tgcggtcatt
    13081 ggctcagtgg gtaaactgcc tacggcagcg attctcggcg gcgtgttgct caaccagaaa
    13141 ctgaatccgg caacgctgga gaacgaatct gacaagcaga aactgatgat cctgctgcgt
    13201 accttctttg aagtgcataa aggctggcat attcagtaca acatcgtttc ccgcgaaacg
    13261 ctgctggagg cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg tgtcgcgggc
    13321 tattccgcct tcttcaccgc gctctctcca gacgcacagg acgatatcat cgcccgtact
    13381 gaacatatgc tgtaatctcc tcggcccggc ggcgtcgtcg ccgggctaaa tcattcacat
    13441 catcaatttc atccttactt tcattcgaaa tataatttgt gctctgcgtc acattgttat
    13501 tacactgtta cggtttcatt ttcgagccag gagcacacct atgagcgtaa aagttatcgt
    13561 cacagacatg gacggtactt ttcttaacga cgccaaaacg tacaaccaac cacgttttat
    13621 ggcgcaatat caggaactga aaaagcgcgg cattaagttc gttgtcgcca gcggtaatca
    13681 gtattaccag cttatttcgt tctttcctga gctaaaggat gagatctctt ttgtcgcgga
    13741 aaacggcgca ctggtttacg aacatggcaa gcaactgttc cacggcgaac tgacccgaca
    13801 tgaatcgcgg attgttattg gcgagttgct aaaagataag caactcaatt ttgtcgcctg
    13861 cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg cactgatggc
    13921 aaaacactac catcgcctga aagctgtaaa agattatcag gagattgacg acgtactgtt
    13981 caagttttcg ctcaacctgc cagatgaaca aatcccgtta gtgatcgaca aactgcacat
    14041 agcgctcgat ggcattatga aacccgtcac cagtggtttt ggctttatcg acctgattat
    14101 tcccggtcta cataaagcaa acggtatttc gcggttactg aaacgctggg atctgtcacc
    14161 gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg gagatgctga aaatggcgcg
    14221 ttattccttt gcgatgggca atgctgcgga aaacattaaa caaatcgccc gttacgctac
    14281 cgatgataat aatcatgaag gcgcgctgaa tgtgattcag gcggtgctgg ataacacatc
    14341 cccttttaac agctgacctc tctaccggag ccgtgcgctc cggttttccc ttctttttac
    14401 atcctgtaca tcacatcaaa tcattaaact tgcattaact tatttataac ttatattgtc
    14461 gcatgcaaat cagaatggtt tcgaatgaaa gttaatgaga ggtggttatg gcttctactt
    14521 ttaccagcga cacattgcct gccgatcaca aagcagctat ccgtcagatg aagcacgcgc
    14581 tgcgggcgca gcttggcgac gtccagcaga tctttaatca gctaagcgat gacattgcca
    14641 cgcgagtggc tgaaatcaac gcactcaaag cacagggcga tgccgtctgg ccggtgctgt
    14701 cttatgccga tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag attaaacgtc
    14761 gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc tgggatcagt
    14821 cgatgctgga ctatctggac cgcaaccgct ttgacgaggt ctacaaaggc cccggcgata
    14881 atttcttcgg gacgctcagc gcttcacgtc ccgagattta ccccatctac tggtcgcagg
    14941 cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt ctcaatcgtc
    15001 tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc gtcatctacc
    15061 ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa aggtcttgga gcgcataccg
    15121 actccggggc gctggaacgc tggctgcttc cagcgtatca gcgcgttttc gccaacgtct
    15181 ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc acatcgtacg gaagttgaag
    15241 agtacacggt ggacaacacc accaaatgtt ccgtgtttcg gacattccaa ggctggacag
    15301 cgctctctaa tatgctgcct ggtcaggggc tgctgcacgt tgtgcccatt cctgaagcca
    15361 tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa ctgtgcggcg
    15421 tagcgcccgg aagagtgttg ccggtatcag agcaatggca tccactgtta attgaggcgt
    15481 taaccagcat tccaaaactc gaggccggag actccgtctg gtggcactgc gacgtcatcc
    15541 attccgttgc ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac attcctgcgg
    15601 caccaatgtg cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca ctggaaaaag
    15661 gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac aaactgggaa ggacgcttta
    15721 cgcttgccga cctcaacatt cacggtaagc gagcgttggg aatcgacagc tgagtgtcgt
    15781 acagcccttc ccgttccacg gcggtgttcc cttttcctgg gcgaacccgc cgcactgact
    15841 cccgcaccgc caatcgtccg tgcagcagca gcgagcccac cggagcatcc gggcgaatca
    15901 gccgctgttg caacatttgc accgcttcat tgcctagctc atcgcgtggc acatgcacag
    15961 ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc gtcgatactc atcaccgaga
    16021 catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt taccgttccc gccgccataa
    16081 aatccccgcc gaccagaaac gccgtcggca acgtcttgcc attggcggca tccagccacg
    16141 ctccgacctt ttcttccgct tctttagcgc taaaactgct gatattcagc aaatgacggt
    16201 catggtgaaa ggccagatta tgctgacgcc atgattcttt aatccctgca aaccgctgct
    16261 ccatagtgta gcggcgcagg cacatcacat tcatcacctc ccggtgaccc atttcaaaca
    16321 gatagcgggc ggcgaacgcg ccaattaacc gatgatccgg cgcaatcgcc gggaggcgca
    16381 ttcgttcatc acggcagttg atcagcacac aaggcttagc gagatcggcg gccagatcat
    16441 ggatgtgcgg atcgtcaatc cccagcagga tcgctgcctg cgtatctgct tcattcatcc
    16501 gcgagagaaa gagcgcggga tcgctgtcaa actcctccag cgcgcagtaa cgcagccgca
    16561 cttcatgcgg cgataacgct ttgctgatgc tctggatcac acggtaataa aagatatcag
    16621 atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag gttgttcagc aacatacgac
    16681 ctgctgccat gccatccatc acgcccaact cacgcgcgca ggccagcacc tgttcgcgag
    16741 cgcgtgcact ggtattcgct ttgccagcca gcacccgcga aaccgtactc ggcgatagcc
    16801 cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc cattttgtga tctgcctcta
    16861 attcggaaat gacagaattt tcatgagcaa aaaagcccat tctgtctggc attaaatcca
    16921 tttcagtaat gtatatcagt cactcatgga aaaacgtgca caaaacccgc tttctttcgc
    16981 ttttagggtc tcttactgtg aattggtaga acaacttcca tacaaaaata atggaaaaac
    17041 gaacaataaa gactttattt atcaaaaagt taaaagtggt gtaacaactc cattctgacg
    17101 ttgccgcaca aaccctatac cattctgtgg agtgcatatg agtcagggta tcaataacga
    17161 tatgactgcc agtaaaccgc gccatatcgt caaaaatctg cgttggtgga tactggtcct
    17221 ttttttgctc ggtgttactg tcaactacat cacgcgtaac tcgctgggta ttctcgcccc
    17281 cgaactgaaa agcagcctag ggattaccac cgaacaatat tcctggattg taggtgcttt
    17341 ccagctcgcc tataccctat tccaaccgtt gtgcggatgg ctgattgacg tcattggcct
    17401 caaactgggc tttatgatct gcgcgggact gtgggcactg gcctgtctga tgcatgcggg
    17461 ggcgggaaac tggggacatc tggcgctgtt gcgcttcttt atgggcggtg cggaagccgc
    17521 ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt ccaaaatcag aacgcccgat
    17581 cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc atgctggccc caccgattat
    17641 ctattttgcc cacgcttcat tcggctggca gggtgcattt atgttcactg gcatcctggc
    17701 gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacagc ccggataagc atcctaacct
    17761 cagcaaagcc gagctggact ttattcgcca ggataacgaa gcgcccccgg tcaagttacc
    17821 ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc ttttacggta tcgctatccc
    17881 ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc tgggtgcctc tatacctgtc
    17941 caaaacctat ggcatggacc tgaagcagat cgcattattc gcctggttgc cgttcctcgc
    18001 cgctgatctt ggcagtattg ccagcggcta tctgaccaaa ctttacaccc gcctgtttgg
    18061 gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt ggcgcattct tgatggtttc
    18121 gctggcggtg atggccttta ccaataaccc gtatatcgcc atcgtcctta tctccatcgg
    18181 cggcttcggt caccagatca tctcctgcat gctgagcgca ctggtggtgg aatccttcga
    18241 caaaggtcag atggccactg ttaacggcat gcgcggttct gccgcgtgga tcgcaagttt
    18301 cctgttctcc ctgattatcg gggttaccgc tgacaaaatc ggctttaacc ccctgtttat
    18361 cgccatgggc ttctttgacc tgattggcgc tttcttcctg gtcaccttta ttgctgaacg
    18421 tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa cgctgaaaaa ctggaccctg
    18481 cacaaacagt ctgcccacca cgttgaactg ctggtcgatg gccagcatat cctgtgcctg
    18541 tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac gcaaaggcga gctggcgcta
    18601 gatcgcacct ggagcattgc tccggcgcag gatgtgccat gggaagggcg cagccgcgaa
    18661 gatctcagcg gctttagcct gcccgcgtgg acagtgatcc agcacgacga caccgtggtt
    18721 atcgccaccg agtcactgcg tgcaaccgtt caccagccgc tgtggctgga gtggcactat
    18781 cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc gcccgaccag cgcttatctg
    18841 ctgaatgccc acggcgatgg cgtggcacac tatcagagcc gtcgtaaaga tgaacgcttt
    18901 tatggcctcg gagataaagc cggcgatctg caacgcaacg gtaaacgtta cgagatgcgt
    18961 aacctcgacg cgatgggcta caacgccgtt agtactgacc cgctgtacaa acacattcca
    19021 ttcaccatca cccgccgcga tgacattagc ttcggcctgt tttatgacaa cctcagcagc
    19081 tgctggctgg atctgggcaa cgagatcgac aactaccaca ccgcctatcg ccgctggcag
    19141 gcggaagcgg gtgatatcga ctactacctg tttaccggcg aacgcgtact tgatatcacc
    19201 aaagccttcg tacgcctgac cggaaaaacc ctgttcggac cgaaatggag cctgggctac
    19261 agcggttcaa ctatgcacta caccgatgcg ccggacgcac aaaatcagtt gatgaacttt
    19321 atccgcctgt gtgaagaaca tgcaattccc tgcgattcgt tccagctctc ttccggttat
    19381 acctcaatta acggcaaacg ctatgtgttc aactggaact acgacaaagt gccgcagccg
    19441 aaagtgatga gccaggcatt tcacaatgct ggtttgcacc tggccgccaa tatcaagccg
    19501 tgcctgttgc aggatcaccc acgctataac gaagtggcag aaagaggact gttcatccgt
    19561 gattcagaaa cagatgaacc agaacgttcc agcttctggg atgacgaagg ttctaacctc
    19621 gagtttacta atccgcagac catccagtgg tggcaggaag gcgtgaccac acagcttctg
    19681 gagatgggta tcgactccac ctggaacgac aacaacgaat tcgaagtatg ggacggtgaa
    19741 gcacgctgtc acggctttgg tcagacgatc gcgatcaaac atattcgccc agtgatgcca
    19801 ctgctgatga tgcgagcctc gatggaagcc cagcagcgtt tcgctccgga aaaacgtccg
    19861 tacttgatct cccgctccgg ctgcgcggga atgcagcgct acgtgcagac ctggagtggc
    19921 gataaccgca ctagctggga cactctgcgc tacaacaccc gcatgggtct ggggatgagc
    19981 ctctccgggc tgtataacct cggtcacgac gtcggcggtt tctccggtga taagccggac
    20041 ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc atccgcgctt tacgattcac
    20101 tcatggaacg atgaccatac cgtaaacgaa ccgtggatgt atccgggcgt cactccggcg
    20161 atccgcagcg ctattgagtt gcgctatcgc ctgctgccct acttttatac cctgatgtgg
    20221 caggcatacg ccgatgatga accaatgcta cgtccgactt tccttgacca cgagcacgac
    20281 gttcagactt ttgaagagtg tgatgacttc ctgcttggtc gcgatatttt ggttgccagc
    20341 gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc cagataacga aactggctgg
    20401 tacgatttct acaacggcga atggttcagt ggcggtcagt ggatcaccct cgacgcaccg
    20461 ctggagaaac tgccgctgat ggttcgtgcc ggggctggtc taccgctcag caaacgcatc
    20521 acctatgtga gcgcagaaca agacgatacc cgcgaactga aactgttccc gctgaaaggt
    20581 gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg aaagctgggg ctaccaaact
    20641 ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg gggcgaccat caacctgaaa
    20701 gtcaacgcgc gcggggatta tcgtcctgca tggagtgcac tgaaagtgtc attaccattg
    20761 gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga gtgaatggat gcgttaaatc
    20821 attaccgggg ccgggaggcg actgcacctt acccggtcca gccatcgaat cctttacctc
    20881 agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc cgggagctta aataagtaag
    20941 tgaccggggt gaacaggtga agccaatgca tctgccgcat gaaagacggc ggggaattaa
    21001 gcgtcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcgcgcgt
    21061 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
    21121 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
    21181 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
    21241 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
    21301 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
    21361 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
    21421 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
    21481 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
    21541 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
    21601 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
    21661 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
    21721 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
    21781 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
    21841 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
    21901 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
    21961 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
    22021 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatctc cggatcagca
    22081 aacagcgcct gcgccagaag cacacgcagt ttccagccag gagcaacttc actcatcggg
    22141 ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc
    22201 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
    22261 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
    22321 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
    22381 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
    22441 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
    22501 ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg
    22561 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
    22621 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
    22681 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
    22741 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
    22801 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
    22861 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
    22921 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
    22981 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
    23041 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
    23101 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
    23161 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
    23221 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
    23281 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
    23341 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
    23401 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
    23461 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
    23521 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
    23581 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
    23641 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
    23701 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
    23761 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
    23821 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
    23881 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
    23941 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
    24001 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
    24061 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
    24121 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
    24181 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
    24241 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
    24301 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
    24361 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
    24421 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
    24481 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
    24541 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
    24601 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
    24661 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
    24721 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
    24781 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
    24841 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
    24901 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
    24961 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
    25021 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
    25081 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
    25141 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
    25201 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
    25261 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
    25321 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
    25381 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
    25441 gcgaaggtac aaaaaattaa cgttttagca atagctacat aatatagcct gtgctatatc
    25501 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
    25561 aatgagttca agaagtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
    25621 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
    25681 aaatagcggc gtcgcggtac gccgcttcac tcctgctttt atgcaggcat aacgcgtttt
    25741 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
    25801 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctacgt atattgcggc
    25861 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
    25921 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
    25981 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
    26041 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
    26101 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
    26161 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
    26221 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
    26281 tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa tagcaatcag
    26341 gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
    26401 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
    26461 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
    26521 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
    26581 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggcg aaagcgcgcg
    26641 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
    26701 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcacttgg
    26761 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
    26821 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
    26881 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
    26941 taatttcgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
    27001 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
    27061 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
    27121 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
    27181 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
    27241 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
    27301 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
    27361 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
    27421 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
    27481 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
    27541 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
    27601 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
    27661 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
    27721 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
    27781 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
    27841 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
    27901 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
    27961 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
    28021 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
    28081 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
    28141 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
    28201 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
    28261 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
    28321 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
    28381 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
    28441 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaatag
    28501 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
    28561 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
    28621 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
    28681 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
    28741 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
    28801 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
    28861 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
    28921 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
    28981 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
    29041 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
    29101 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
    29161 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
    29221 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
    29281 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
    29341 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
    29401 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
    29461 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
    29521 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
    29581 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
    29641 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
    29701 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
    29761 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
    29821 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
    29881 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
    29941 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
    30001 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
    30061 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
    30121 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
    30181 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
    30241 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
    30301 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
    30361 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
    30421 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaagga gttgcaaaac
    30481 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
    30541 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
    30601 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
    30661 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
    30721 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
    30781 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
    30841 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
    30901 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgttaa
    30961 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    31021 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    31081 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    31141 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    31201 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    31261 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaagcgca
    31321 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    31381 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    31441 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    31501 cctgtggatt tcggtcctcg gtctggctgg cggtctggta atcggattgc tggcaggttt
    31561 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaggtgat
    31621 ccgcggcaca cctatcgtcg tccaggtgat gtttatttat ttcgccctgc cgatggcgtt
    31681 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    31741 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct attcacaaag gttttcgtga
    31801 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    31861 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    31921 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    31981 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac
    32041 cctggtgcta agctttattt tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    32101 tttaaaaacg tctccaagca ctttggccca acccaggtgc ttcacaatat cgatttgaac
    32161 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    32221 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    32281 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    32341 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    32401 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    32461 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    32521 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    32581 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    32641 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    32701 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    32761 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    32821 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    32881 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    32941 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    33001 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    33061 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    33121 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    33181 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    33241 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    33301 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    33361 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    33421 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    33481 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    33541 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    33601 gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    33661 tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca ggacgagagc gcccgttact
    33721 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    33781 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    33841 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    33901 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    33961 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    34021 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    34081 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcaaaa accatcactc
    34141 tctcgccaca tactcagcgt aactatccag agttgcaaaa acggctgaat ggctggcttt
    34201 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg ttgctgttga
    34261 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    34321 tagatatcct gatccgtatc gcacttattc tttttttctc ggcggttggc tggacggtgc
    34381 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    34441 cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca
    34501 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    34561 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta
    34621 ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    34681 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    34741 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    34801 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    34861 aggcactgaa agatgcggta gcggaattaa tggaaaacga agaaattcgc gggctgatta
    34921 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    34981 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    35041 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    35101 ctactccggg cgcgaccccg gctgaaccgt taccgccggg ggagccaacg ctttaacgct
    35161 ggcgattgac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    35221 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    35281 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    35341 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    35401 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    35461 ccaggttacg gcgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg
    35521 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg
    35581 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    35641 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    35701 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    35761 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    35821 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    35881 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    35941 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat
    36001 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    36061 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    36121 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    36181 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttaatttgtc ttattttgta
    36241 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca tttgcatcgc
    36301 gcaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    36361 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    36421 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    36481 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgaacaa
    36541 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    36601 accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct
    36661 gttttgtaat caaaaaacat caaatatgaa ctcaatgtaa ataaatgtat ttctttttcg
    36721 cgcaatgggt gatagaaaat cgcgccaagt gataatgctt atcaaaatta ttatcacttg
    36781 catgaacact atcacgggat taacagtggc atcgcatccg cagagatgct ttctcgtggc
    36841 agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc
    36901 cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg catcacgcct
    36961 gtggctcagg cactcgccgc cgaagggcaa actaacgcgg atgacacgct ggttgtcgaa
    37021 gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg
    37081 gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc
    37141 gcaaccaacc ttaccgacgc gctcaaaaac gcccccggcg
//
LOCUS       DAJTXQ010000030        36326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000030 DAJTXQ010000000
VERSION     DAJTXQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.030"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OO515_001404"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OO515_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6575372.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OO515_001405"
     CDS             1867..1992
                     /locus_tag="OO515_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575373.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OO515_001406"
     CDS             complement(1989..2060)
                     /locus_tag="OO515_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575374.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OO515_001407"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OO515_001407"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6575375.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO515_001408"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO515_001408"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6575376.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OO515_001409"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OO515_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6575377.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO515_001410"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO515_001410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6575378.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO515_001411"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO515_001411"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6575379.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO515_001412"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO515_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6575380.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OO515_001413"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OO515_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6575381.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OO515_001414"
     CDS             complement(9465..9872)
                     /locus_tag="OO515_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6575382.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO515_001415"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO515_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6575383.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO515_001416"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO515_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6575384.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OO515_001417"
     CDS             complement(11538..12197)
                     /locus_tag="OO515_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6575385.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OO515_001418"
     CDS             complement(12361..12672)
                     /locus_tag="OO515_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6575386.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OO515_001419"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OO515_001419"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6575387.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OO515_001420"
     CDS             complement(14207..14950)
                     /locus_tag="OO515_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6575388.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO515_001421"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO515_001421"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6575389.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO515_001422"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO515_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6575390.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO515_001423"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO515_001423"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6575391.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OO515_001424"
     CDS             20005..20088
                     /locus_tag="OO515_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575392.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO515_001425"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO515_001425"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6575393.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO515_001426"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO515_001426"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6575394.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO515_001427"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO515_001427"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6575395.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO515_001428"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO515_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6575396.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OO515_001429"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OO515_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6575397.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OO515_001430"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO515_001430"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OO515_001431"
     CDS             25234..25836
                     /locus_tag="OO515_001431"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6575398.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OO515_001432"
     CDS             complement(25937..25996)
                     /locus_tag="OO515_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6575399.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO515_001433"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO515_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6575400.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO515_001434"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO515_001434"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6575401.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO515_001435"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO515_001435"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6575402.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OO515_001436"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OO515_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6575403.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OO515_001437"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OO515_001437"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6575404.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OO515_001438"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OO515_001438"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6575405.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OO515_001439"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OO515_001439"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6575406.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OO515_001440"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OO515_001440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6575407.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36326)
                     /locus_tag="OO515_001441"
     CDS             complement(35716..>36326)
                     /locus_tag="OO515_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6575408.1"
                     /translation="IGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDR
                     QLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRF
                     QNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPAT
                     LKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8701 a         9741 c         9170 g         8714 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatca
//
LOCUS       DAJTXQ010000031        35778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000031 DAJTXQ010000000
VERSION     DAJTXQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.031"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..581)
                     /locus_tag="OO515_001442"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OO515_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO515_001443"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO515_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6575409.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO515_001444"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO515_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6575410.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO515_001445"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO515_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6575411.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OO515_001446"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OO515_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6575412.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OO515_001447"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OO515_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6575413.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OO515_001448"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OO515_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6575414.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OO515_001449"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OO515_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6575415.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO515_001450"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO515_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6575416.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OO515_001451"
     CDS             complement(10994..11137)
                     /locus_tag="OO515_001451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575417.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OO515_001452"
     CDS             11138..11416
                     /locus_tag="OO515_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575418.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OO515_001453"
     CDS             complement(11463..13559)
                     /locus_tag="OO515_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6575419.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OO515_001454"
     CDS             complement(13559..14296)
                     /locus_tag="OO515_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6575420.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OO515_001455"
     CDS             complement(14293..14931)
                     /locus_tag="OO515_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6575421.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OO515_001456"
     CDS             complement(15045..15287)
                     /locus_tag="OO515_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575422.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO515_001457"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO515_001457"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6575423.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OO515_001458"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OO515_001458"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6575424.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OO515_001459"
     CDS             19524..19796
                     /locus_tag="OO515_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6575425.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO515_001460"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO515_001460"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6575426.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OO515_001461"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OO515_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OO515_001462"
     CDS             22056..22862
                     /locus_tag="OO515_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6575427.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OO515_001463"
     CDS             22872..23279
                     /locus_tag="OO515_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6575428.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OO515_001464"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OO515_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OO515_001465"
     CDS             23839..24636
                     /locus_tag="OO515_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6575429.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OO515_001466"
     CDS             24647..25471
                     /locus_tag="OO515_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6575430.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OO515_001467"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OO515_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OO515_001468"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OO515_001468"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6575431.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OO515_001469"
     CDS             complement(27914..29545)
                     /locus_tag="OO515_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6575432.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO515_001470"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO515_001470"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6575433.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OO515_001471"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OO515_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6575434.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35778
                     /gene="espL4"
                     /locus_tag="OO515_001472"
                     /pseudo
     CDS             34789..35778
                     /gene="espL4"
                     /locus_tag="OO515_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9230 c         9100 g         8850 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaaggat
//
LOCUS       DAJTXQ010000032        34981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000032 DAJTXQ010000000
VERSION     DAJTXQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.032"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /locus_tag="OO515_001473"
     CDS             complement(<1..815)
                     /locus_tag="OO515_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6575435.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO515_001474"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO515_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OO515_001475"
     CDS             complement(1397..1873)
                     /locus_tag="OO515_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6575436.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO515_001476"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO515_001476"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6575437.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OO515_001477"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OO515_001477"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6575438.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OO515_001478"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OO515_001478"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6575439.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OO515_001479"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OO515_001479"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6575440.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OO515_001480"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OO515_001480"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6575441.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OO515_001481"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OO515_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6575442.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO515_001482"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO515_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6575443.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OO515_001483"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OO515_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6575444.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OO515_001484"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OO515_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6575445.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OO515_001485"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OO515_001485"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6575446.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OO515_001486"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OO515_001486"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6575447.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OO515_001487"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OO515_001487"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6575448.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OO515_001488"
     CDS             13848..14552
                     /locus_tag="OO515_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6575449.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OO515_001489"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OO515_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OO515_001490"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OO515_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO515_001491"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO515_001491"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6575450.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OO515_001492"
     CDS             complement(17484..18245)
                     /locus_tag="OO515_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6575451.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OO515_001493"
     CDS             18378..18788
                     /locus_tag="OO515_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6575452.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OO515_001494"
     CDS             complement(18750..19856)
                     /locus_tag="OO515_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6575453.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OO515_001495"
     CDS             complement(19867..21000)
                     /locus_tag="OO515_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6575454.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OO515_001496"
     CDS             complement(20993..22729)
                     /locus_tag="OO515_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6575455.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO515_001497"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO515_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6575456.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO515_001498"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO515_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6575457.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OO515_001499"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OO515_001499"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6575458.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO515_001500"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO515_001500"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6575459.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OO515_001501"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OO515_001501"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6575460.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OO515_001502"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OO515_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6575461.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OO515_001503"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OO515_001503"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6575462.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OO515_001504"
     CDS             complement(31413..31673)
                     /locus_tag="OO515_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6575463.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO515_001505"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO515_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6575464.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO515_001506"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO515_001506"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6575465.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OO515_001507"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OO515_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8274 a         8975 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAJTXQ010000033        33999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000033 DAJTXQ010000000
VERSION     DAJTXQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.033"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..514
                     /locus_tag="OO515_001508"
                     /pseudo
     CDS             12..514
                     /locus_tag="OO515_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO515_001509"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO515_001509"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6575466.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO515_001510"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO515_001510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6575467.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO515_001511"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO515_001511"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6575468.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO515_001512"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO515_001512"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6575469.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO515_001513"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO515_001513"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6575470.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO515_001514"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO515_001514"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6575471.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO515_001515"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO515_001515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6575472.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO515_001516"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO515_001516"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6575473.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO515_001517"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO515_001517"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6575474.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO515_001518"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO515_001518"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6575475.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO515_001519"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO515_001519"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6575476.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO515_001520"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO515_001520"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6575477.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO515_001521"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO515_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6575478.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO515_001522"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO515_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6575479.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="OO515_001523"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="OO515_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6575480.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="OO515_001524"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="OO515_001524"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6575481.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="OO515_001525"
     CDS             complement(20046..21878)
                     /locus_tag="OO515_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6575482.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO515_001526"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO515_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="OO515_001527"
     CDS             complement(22624..23118)
                     /locus_tag="OO515_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6575483.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="OO515_001528"
     CDS             complement(23201..23656)
                     /locus_tag="OO515_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6575484.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="OO515_001529"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="OO515_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6575485.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="OO515_001530"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="OO515_001530"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6575486.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="OO515_001531"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="OO515_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6575487.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO515_001532"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO515_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6575488.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO515_001533"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO515_001533"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6575489.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO515_001534"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO515_001534"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6575490.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="OO515_001535"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="OO515_001535"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6575491.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="OO515_001536"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="OO515_001536"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6575492.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="OO515_001537"
     CDS             32232..33125
                     /locus_tag="OO515_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6575493.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO515_001538"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO515_001538"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6575494.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8480 a         9277 c         8564 g         7678 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agtacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatagccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAJTXQ010000034        33988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000034 DAJTXQ010000000
VERSION     DAJTXQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.034"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..2594)
                     /gene="acrF"
                     /locus_tag="OO515_001539"
                     /pseudo
     CDS             complement(1..2594)
                     /gene="acrF"
                     /locus_tag="OO515_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2606..3763)
                     /gene="acrE"
                     /locus_tag="OO515_001540"
     CDS             complement(2606..3763)
                     /gene="acrE"
                     /locus_tag="OO515_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6575495.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4162..4824
                     /gene="acrS"
                     /locus_tag="OO515_001541"
     CDS             4162..4824
                     /gene="acrS"
                     /locus_tag="OO515_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6575496.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4827..5006)
                     /locus_tag="OO515_001542"
     CDS             complement(4827..5006)
                     /locus_tag="OO515_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6575497.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5090..5974)
                     /gene="yhdJ"
                     /locus_tag="OO515_001543"
     CDS             complement(5090..5974)
                     /gene="yhdJ"
                     /locus_tag="OO515_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6575498.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6060..6356)
                     /gene="fis"
                     /locus_tag="OO515_001544"
     CDS             complement(6060..6356)
                     /gene="fis"
                     /locus_tag="OO515_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6575499.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6382..7347)
                     /gene="dusB"
                     /locus_tag="OO515_001545"
     CDS             complement(6382..7347)
                     /gene="dusB"
                     /locus_tag="OO515_001545"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6575500.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7676..8557)
                     /gene="prmA"
                     /locus_tag="OO515_001546"
     CDS             complement(7676..8557)
                     /gene="prmA"
                     /locus_tag="OO515_001546"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6575501.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8569..10020)
                     /gene="panF"
                     /locus_tag="OO515_001547"
     CDS             complement(8569..10020)
                     /gene="panF"
                     /locus_tag="OO515_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6575502.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10010..10252)
                     /locus_tag="OO515_001548"
     CDS             complement(10010..10252)
                     /locus_tag="OO515_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6575503.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10361..11710)
                     /gene="accC"
                     /locus_tag="OO515_001549"
     CDS             complement(10361..11710)
                     /gene="accC"
                     /locus_tag="OO515_001549"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6575504.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11721..12191)
                     /gene="accB"
                     /locus_tag="OO515_001550"
                     /gene_synonym="fabE"
     CDS             complement(11721..12191)
                     /gene="accB"
                     /locus_tag="OO515_001550"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6575505.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13169..14143)
                     /locus_tag="OO515_001551"
     CDS             complement(13169..14143)
                     /locus_tag="OO515_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6575506.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14295..16235
                     /gene="csrD"
                     /locus_tag="OO515_001552"
     CDS             14295..16235
                     /gene="csrD"
                     /locus_tag="OO515_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6575507.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16540..17583
                     /gene="mreB"
                     /locus_tag="OO515_001553"
     CDS             16540..17583
                     /gene="mreB"
                     /locus_tag="OO515_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6575508.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17649..18752
                     /gene="mreC"
                     /locus_tag="OO515_001554"
     CDS             17649..18752
                     /gene="mreC"
                     /locus_tag="OO515_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6575509.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18752..19240
                     /gene="mreD"
                     /locus_tag="OO515_001555"
     CDS             18752..19240
                     /gene="mreD"
                     /locus_tag="OO515_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6575510.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19249..19842
                     /gene="yhdE"
                     /locus_tag="OO515_001556"
     CDS             19249..19842
                     /gene="yhdE"
                     /locus_tag="OO515_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6575511.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19832..21301
                     /gene="rng"
                     /locus_tag="OO515_001557"
     CDS             19832..21301
                     /gene="rng"
                     /locus_tag="OO515_001557"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6575512.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21369..25169
                     /gene="yhdP"
                     /locus_tag="OO515_001558"
     CDS             21369..25169
                     /gene="yhdP"
                     /locus_tag="OO515_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6575513.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25325..26770
                     /gene="tldD"
                     /locus_tag="OO515_001559"
     CDS             25325..26770
                     /gene="tldD"
                     /locus_tag="OO515_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6575514.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26898..27763)
                     /locus_tag="OO515_001560"
                     /pseudo
     CDS             complement(26898..27763)
                     /locus_tag="OO515_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27946..28149
                     /locus_tag="OO515_001561"
     CDS             27946..28149
                     /locus_tag="OO515_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6575515.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28157..29089
                     /gene="aaeA"
                     /locus_tag="OO515_001562"
     CDS             28157..29089
                     /gene="aaeA"
                     /locus_tag="OO515_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6575516.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29095..31062
                     /gene="aaeB"
                     /locus_tag="OO515_001563"
     CDS             29095..31062
                     /gene="aaeB"
                     /locus_tag="OO515_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6575517.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31154..31426
                     /locus_tag="OO515_001564"
     CDS             31154..31426
                     /locus_tag="OO515_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6575518.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31482..31745)
                     /gene="yhcN"
                     /locus_tag="OO515_001565"
     CDS             complement(31482..31745)
                     /gene="yhcN"
                     /locus_tag="OO515_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6575519.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32110..32580)
                     /gene="argR"
                     /locus_tag="OO515_001566"
     CDS             complement(32110..32580)
                     /gene="argR"
                     /locus_tag="OO515_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6575520.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33015..33953
                     /gene="mdh"
                     /locus_tag="OO515_001567"
     CDS             33015..33953
                     /gene="mdh"
                     /locus_tag="OO515_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6575521.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8251 a         8451 c         8897 g         8389 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacgtaaa aacaacacca ccattcctgc aacaatcagc
      961 gcatagatca gtaaatatcg tcctgtggaa ccgaggattt tgccgacgct gttggtgtag
     1021 tggttaacgc tatgatcgaa ggtggtatta aaccagccga agaaaccgcc cttattttcg
     1081 tgatgctcag cagagacggg tttaagcagc gttgcacata acgcaggggt aagaatcaat
     1141 gccaccagaa cagaaagcgc cattgccgaa acgatggtga tagagaactg gcgataaatt
     1201 gccccagtag aaccgccgaa gaatgccatc ggaataaata ccgctgacag caccatcgcg
     1261 atacccacca gtgcgccctg aatttgcgac atcgattttt ccgtcgcttc ttttggcggg
     1321 agcttatcct ccatcatcac gcgctcgacg ttctccacca ccactatcgc atcatcgacg
     1381 agcagcccga tggcaagcac catcccgaac atcgttagtg tgttgatgga gtaaccaaaa
     1441 gcggcgagga tggcaaacgt ccctaacaac accacgggta ccgcaatggt ggggatcagc
     1501 gttgctcgca tattctgcaa gaacagatac atcaccagga acaccagcat aatggcttcg
     1561 aacagcgttt ttaccacttc gtgaatagaa agctggacga atggcgtggt gtcataaggg
     1621 tagagaacct tcattccctg cgggaagaat ggctgtaatt ccgccagttt tgccttaatg
     1681 gctttcgcgg tattgagagc gttcgcgccg gttgctagct taatccccag gcccgccgcc
     1741 ggttttccgt tgatacgagc gataacgtta tagttttcac cgccaagttc aactcgtgcg
     1801 acatctttca ggcgtaccac cgagccgtca ctgtttacgc gcagggttac tttgccgaat
     1861 tcttccggat tttttaaccg cgtctgagca ataatcgagg cgttcaactg ttgccctggt
     1921 aacgctggcg ttccgcccaa ctgcccggca gcgatctgat cgttctgtac cttcaactgg
     1981 ttaatcacat caaccggtgt cagtttatat ttgtttagca gatcggcgtc cagccagata
     2041 cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga cgccattcag acggctaagc
     2101 gtatctttaa cgttagaggc cacatagtcc gagatatcgt cctgtgtggt gtctgggtta
     2161 tcagagacaa agcccgccac catcaaatag ctgctactgg acttttcaac actgatcccc
     2221 tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct ggagtttgtt ctgcacctgc
     2281 acttgcgcga tatcaggatc ggtcccggac tggaaggtaa gggtaattgt cacgctaccg
     2341 gcggaatcgc tggtggagga catatacatc aggttatcga taccattcat attctgttcg
     2401 ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag cacctggata gtttgcctaa
     2461 acagaaaccg caggcggtgc aatggttgga tactgagcga cgggcaattg taggatcgcc
     2521 agtgcgcccg ccatcatcag aataatggcc agcacccatg caaatatcgg tcgtcgaata
     2581 aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt atctgcgggg gtatcggtag
     2641 tggctttaac ctgctctccc ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc
     2701 cagatttcag accttcgcta atcaaccatt tatcgccaat cgcctgactg gcaacgacag
     2761 ggcgcgcttc cacctgactt ttatcgttaa caatcagcac ggttgcatca ccacgcggtg
     2821 tgcggctaac gccttgttgc gggataagaa tggcgtcagg ttggacgcct tcatcaatcc
     2881 gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg gttagggaag acagcacgta
     2941 gggttatgga gccggtgcat tcatcaacgg tcacatcaga gaattgcagc gtacctttca
     3001 ggggataggt ttgaccgttt tccatgacca actctacgtt gctggtggcg ttttccttat
     3061 gcaaatttcc ttgctctacg gattgcttca gcctcataaa atcgttgctg gattgggtca
     3121 catcaacgta gataggatcg agctgctgga cagtcgccag ttcagtcgtt tgcccattag
     3181 tgacaagagc gccttcggtc acggtcgatt tgccgatacg tccgctaatt ggcgcagtca
     3241 ctttggtata agcgagattg atgcgagcgc tttcgactgt ggcttttgcg gcaatcacgg
     3301 cggcatcggc ctggcgagca tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt
     3361 tcgtacccac gagcggaacg taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt
     3421 cacttttcgc cagttcgcct ttcgcgctgt cataatttgc ctgataggtc gcgggatcga
     3481 tctggtacag ggactggcct gcttgtacat cgctgccttc agtgaaatta cgattcagta
     3541 cgatcccgct aacctgtggg cgaacttcgg ctatacggta agcattggtg cggcctggta
     3601 attcagtctt aacttctaac ggggccgttt tcacaatatg aacggtaacc tgcggttcac
     3661 cgacgtgagc tttctcttct cccttatcgt tacaaccgac gattaaagcc gcggagatca
     3721 gaataaagga gggcaggagg aaaaacctgg catgtttcgt cattactatt cctcaaaaaa
     3781 acaaaagcgc gttatttacc cagaaaggca acgcgttaac tcgcagaaag aaaaatacag
     3841 tttgctatcc tacaaattat cattcgtcga tgtaaggaat agttatgaat actggcgtct
     3901 caaggcacat aaacacaaaa aaagattaat attctactgt tttattttga cgcgggttga
     3961 aagaggcaga attaaaacct cgtaaattga aatatatatt catgtggcga atgtatttta
     4021 ggtaaataat atatattatt tatttgacga tttatagcaa ctcaatgtta ccctctcgta
     4081 taaatacaca ttagatgata gattaacctt cgctattttc tcaccctgtg tcgaatatat
     4141 ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct gaagacccgg
     4201 caagaactga ttgaaactgc catcgcccag tttgcgcaac atggcgtaag caagacgacg
     4261 ttcaacgaca ttgccgatgc cgctaacgtt acgcgtggcg ctatctactg gcacttcgag
     4321 aacaagaccc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc
     4381 caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg
     4441 attgtcggct tgcaatatat tgccaaaatt ccccgccagc aggcgttgct gaaaatctta
     4501 tatcacaaat gtgaatttaa tgatgggatg ctggccgagg gagtgatacg cgaaaagatg
     4561 ggctttaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta
     4621 gcaaataacc tcgatttaga tgttgtgatg attattattg atggtgcctt cagcggaatt
     4681 gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc acccgcgctg
     4741 gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa
     4801 acaaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag
     4861 ttaaccagtt ttagtggata aactgagttc atattacggc atttgtctgt ttctgttgtc
     4921 agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg
     4981 acgagccacc aatacttgcg aatcattacc tgagcctgtg catgtttaac caagtctggc
     5041 aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa
     5101 tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt
     5161 ttttcacttt cgccagttct tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc
     5221 ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg
     5281 ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg
     5341 aagaggcgag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat
     5401 attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat
     5461 gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt
     5521 tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca
     5581 tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat
     5641 atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg
     5701 gcatgttttc cgtactgttc ataatgtaca tgctgccctg ctttttcaga acgcggtggc
     5761 actctacaat cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca
     5821 gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagatcaga tcgacgcttt
     5881 cagtaggtag ctttttaagt tcggcaaggg catcaccgtg aataatggtc ttagcttcat
     5941 taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa
     6001 aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat
     6061 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     6121 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     6181 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     6241 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     6301 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     6361 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     6421 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     6481 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     6541 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     6601 cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     6661 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     6721 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     6781 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     6841 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     6901 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     6961 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     7021 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     7081 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg
     7141 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     7201 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     7261 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     7321 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     7381 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     7441 gatggatcaa agaattgcat ttaaaatgag cgtggtgaga taattactca taaaaatcat
     7501 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     7561 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataaccggca aatttacgtg
     7621 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat
     7681 tcttacgacc ggtaatgcgg caccactctt ctttttccac gaccgggtcc agtgcgaagc
     7741 tgtcgacata agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca
     7801 gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg
     7861 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     7921 agagttccag acggtcagaa acgccattac gttcggcgtt gtcgcggcta gcctgaatcg
     7981 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatag
     8041 ccagaatgcc ggaaccacag ccaaagtcga tgactatttt accggttaaa tcgaggctgt
     8101 cgagccactg caggcacaga gaggtagttg gatgggtacc cgtaccaaac gccagccctg
     8161 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     8221 acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     8281 tatcttccag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     8341 gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt
     8401 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     8461 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tcagcgttcg
     8521 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     8581 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     8641 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     8701 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     8761 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     8821 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     8881 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     8941 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgtag
     9001 caattgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc
     9061 agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag
     9121 atcggggatc actgcacgac ctaacgctcc ggctaggtgc ataccgaaca tcaggattgc
     9181 gactacaatc gtaccgatga tgatcccccg gtgtaccgct ttgctgtctt tataagagat
     9241 acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga
     9301 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
     9361 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
     9421 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
     9481 ggcgcgaaag ccgccaaagg cggtatacaa cgcaatgctg ataccgaaaa tcagcagccc
     9541 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
     9601 ggtcattgcc ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
     9661 actctggtag cgggcaaaca gcatgtcgtt cagcgtcact gcattgtagc ggcgcgcaag
     9721 aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat
     9781 cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga
     9841 actggcactg atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg
     9901 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
     9961 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
    10021 cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
    10081 taaacagtag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
    10141 cgggggcaac gccaggtaag taagcggcta ctaaccaaac tgccagatac aaaagggtca
    10201 gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
    10261 cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa
    10321 aaaaggccgg aaaatccggc cttttggcgc ttttgcagtc ttatttttcc tgaagaccga
    10381 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
    10441 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
    10501 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
    10561 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
    10621 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
    10681 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
    10741 tgatcgacag cggttgaccg gcagcgatac ggagctgttc tttgatcagg tcaacgccag
    10801 tgatcatttc ggtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
    10861 actcgccgtt ttcgaacaga aactcgaaag tacctgcacc gcgatagccg atatcaacac
    10921 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
    10981 gcgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
    11041 agatagcgtt gccccgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
    11101 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
    11161 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
    11221 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
    11281 tatccatatc gtcgcccaac gggccgtcag aacccggtac gcaagggacg cccgcttttt
    11341 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
    11401 cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
    11461 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
    11521 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
    11581 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
    11641 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
    11701 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
    11761 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
    11821 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
    11881 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
    11941 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
    12001 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
    12061 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
    12121 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
    12181 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
    12241 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
    12301 gggacgccgg tctattttgc cccagagtat caatgttaga cttgacggac attgtgcagc
    12361 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
    12421 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
    12481 caattggcag ctaaatactg gtctcatcag ggaagataat caacagctaa catgtaaata
    12541 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
    12601 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
    12661 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
    12721 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
    12781 tagatgtgaa tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg
    12841 ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg taccagaaat
    12901 cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
    12961 aggcgtatca ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg
    13021 aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga
    13081 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt
    13141 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
    13201 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
    13261 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
    13321 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
    13381 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
    13441 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
    13501 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
    13561 tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
    13621 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
    13681 cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
    13741 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
    13801 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
    13861 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
    13921 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
    13981 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
    14041 gaacatcgac cgtgacatcg ccctccggca ggcgactttc gtccagagtc tgtactgatg
    14101 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
    14161 atgatggatt ggaaaataat ttctgaagat tatactcgct aatggaaaag caaagccgat
    14221 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
    14281 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
    14341 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt
    14401 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
    14461 atgacttcag cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc
    14521 gtgtagacct gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc
    14581 gtccggttgg ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg
    14641 ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga
    14701 ctaccgcgcc gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac
    14761 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
    14821 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
    14881 gcagtgcgct agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
    14941 ttgatacgct gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac
    15001 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
    15061 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca
    15121 gccaggttga agaacagttc ttcactctga ctaatctgct gtcgacattt atgatgcgct
    15181 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc
    15241 ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc
    15301 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta
    15361 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    15421 tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta
    15481 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    15541 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    15601 tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    15661 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    15721 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc
    15781 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    15841 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    15901 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    15961 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca
    16021 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga
    16081 ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag accctgattc
    16141 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    16201 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    16261 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    16321 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    16381 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    16441 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    16501 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    16561 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    16621 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    16681 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    16741 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    16801 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    16861 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    16921 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    16981 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    17041 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg
    17101 cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    17161 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg
    17221 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    17281 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    17341 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    17401 gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    17461 atggaagaaa ccggcattcc agtagttgtt gctgaagacc cgctgacctg tgtggcgcgc
    17521 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    17581 taatcggatg caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg
    17641 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    17701 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    17761 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    17821 aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc
    17881 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    17941 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    18001 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    18061 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    18121 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    18181 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    18241 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    18301 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    18361 tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg
    18421 caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta
    18481 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    18541 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    18601 tcgctcagcc gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc
    18661 ccacacagcc tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc
    18721 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg
    18781 ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa
    18841 cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt
    18901 gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    18961 cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    19021 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    19081 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    19141 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    19201 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    19261 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg tgtgaccttt
    19321 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    19381 gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc
    19441 ccggtgctgg gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc
    19501 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    19561 acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg
    19621 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    19681 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    19741 ggcagctatc acgccgtagt cggcttacct ctggttgaaa cgtatgaatt attaagtaat
    19801 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    19861 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    19921 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    19981 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    20041 atccgatatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    20101 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    20161 tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    20221 ggtctttatg ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga
    20281 acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    20341 ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    20401 acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta
    20461 cggcgaactg gcgctggctc agcgtgttct gcgtgatttc gccgacgccg aactggaccg
    20521 cattcgcgtt gactcacgcc tgacttacga agcattactt gagttcacct cggagtacat
    20581 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    20641 tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg
    20701 ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    20761 tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc
    20821 tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga
    20881 tatgaataat gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa
    20941 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    21001 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    21061 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    21121 tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga
    21181 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    21241 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    21301 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    21361 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    21421 tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc
    21481 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    21541 cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa
    21601 aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    21661 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    21721 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    21781 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt ttcctgacgc
    21841 catccggtca gcgtgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    21901 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    21961 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac
    22021 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc
    22081 tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    22141 gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata
    22201 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct
    22261 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    22321 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    22381 ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    22441 aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca
    22501 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    22561 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    22621 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    22681 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    22741 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    22801 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    22861 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcactatttc ccggaaaact
    22921 tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata
    22981 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    23041 agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    23101 cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    23161 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    23221 cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    23281 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    23341 acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgag ctggtaaccg
    23401 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc
    23461 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    23521 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    23581 cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc
    23641 ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    23701 atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg
    23761 tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg
    23821 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc
    23881 atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg
    23941 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc
    24001 cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg
    24061 gtgcagcgag tttcccacaa tacataacgt tacgtacgcc tatgttgtca ctgggaaatc
    24121 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg
    24181 cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga
    24241 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    24301 catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac
    24361 gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca
    24421 tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta
    24481 ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg aaaggaaaac
    24541 tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc
    24601 agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg cagccagatg
    24661 aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    24721 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc
    24781 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    24841 ccgcgtggat taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg
    24901 cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg
    24961 ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca
    25021 ttgttggcgc ggcagttttt gccgctagta aagtgctggg gccgctgtgg agcaaagtct
    25081 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    25141 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    25201 atatgataat cgttgccaaa ggccaacgag ccagaacata accgtaggcc ggatagggcg
    25261 ttcatgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    25321 aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacagcc tgaaacatca
    25381 ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    25441 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    25501 ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc
    25561 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    25621 cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    25681 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    25741 tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga ctgccagcct
    25801 cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt
    25861 ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg
    25921 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg
    25981 cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc
    26041 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    26101 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc
    26161 agtatttagt gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    26221 cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca
    26281 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    26341 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    26401 catgccgcgt atgactaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat
    26461 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac
    26521 ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac
    26581 gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc
    26641 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    26701 aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg
    26761 tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg
    26821 gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg
    26881 taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta
    26941 ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc
    27001 ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc
    27061 aggatctcca gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg
    27121 atcccggcac ccgccgtcag ccagcgcacc agcgtcatcg gatcattagt cacaaatctt
    27181 ccttgtggga tcaggcgagt cgagatccct tccggtgcga tcagttcaaa ttcattgtcg
    27241 ggccgcacgc tgtattcaag ccatgaatga ctactcaaat cggcgggttt ttccggtatg
    27301 ccgtattgtg tgagatagct tttcgcggcg cacaccacca tcggcatcgc gcccagacgg
    27361 cgggaaaaca ggctggaatt ccggtaacca gattgacgct caaacctggg tattctttca
    27421 gcatttttgc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc
    27481 gcagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt
    27541 catgaagcat ccgacggcag ccctggtagt aaattctgcc ggcttcggtc aggccaatgc
    27601 tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct
    27661 gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt
    27721 caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa
    27781 aagtgattta gatcacataa tatagataac agcataacag ttacgctaat atattaaata
    27841 tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat
    27901 gctgaaaata ataacgcctg ctctctcttt acaaccaagg tcaacatgag tctgtttccc
    27961 gttatcgtgg tgtttggact gtccttccca ccgatatttt ttgaattgct tttatcactg
    28021 gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga ctttgtctgg
    28081 catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg
    28141 ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt
    28201 cgtattagtc attctagcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc
    28261 cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc
    28321 tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag gacagatact
    28381 gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc
    28441 ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg
    28501 tgtgcaggcg atgtctcgcg aagagatcga ccaggccaac aacgtactac aaacggttct
    28561 gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgcac
    28621 ggtgatccgc gcgccagcag atggctgggt gaccaacctc aacgtctata ccggtgagtt
    28681 tattactcga ggatcaacgg cggttgcgct ggtgaaacag aactccttct atgtactggc
    28741 ctatatggaa gaaactaagc tggaaggggg gcgtccgggg tatcgtgcag agatcacgcc
    28801 gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgcag gggtcaccaa
    28861 cgccagcagc acgcgtgacg acaaagggat ggcgactata gactctaatc ttgaatgggt
    28921 gcgtcttgcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag agaacatctg
    28981 gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg acgaaagcca
    29041 ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat catgatgggt
    29101 attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac cgccattgta
    29161 ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca
    29221 gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct
    29281 attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg
    29341 gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg
    29401 gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta cgcgtggggg
    29461 ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc attgcttacg
    29521 ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcgattatg
    29581 gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tagatcgaga gctggaaagt
    29641 ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt
    29701 gtcgataaag cctggggcga cctggtgcga cgcaccacgg cactacaagg catgcgcagc
    29761 aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat
    29821 acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc
    29881 ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga aaccgcgcag
    29941 gacgtccaca agcagctcaa acgcctgcgg agagttatcg cctggaccgg ggaacgggaa
    30001 acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc
    30061 ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg
    30121 gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg ttaacttctg gcgaaccaca
    30181 ctttcctgca ttctgggcac gcttttctgg ctgtggacgg gctggacttc cggcagtggt
    30241 gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa tccacgcatg
    30301 gtggcgatcg actttatcta tgggacgctg gccgcgctgc cgttagggct gctctacttt
    30361 ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag cctggcagtg
    30421 ctgggattct tcctcggtat agaagtacag aaacggcgac tgggctcgat gggggcactg
    30481 gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt cagtcagttt
    30541 ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg
    30601 gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttttgcc
    30661 gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct cccggcactt
    30721 tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa atttcgcctg
    30781 gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc ggttaacgag
    30841 gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat atctgcccgt
    30901 agcgatgata aacgtcgtcg gtactttggc cagttgctgg aagaactgga aatctaccag
    30961 gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccggttca tcggctggcg
    31021 gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac cgacgccaaa
    31081 agcgtcggtt ttttcatggc tatacttagc gatgcacggc agaactcgcc gcgaaacgtg
    31141 acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga
    31201 tttttatcgt gactttagcc aaacctttgg tctggctaaa gataaggtac gcgatctcga
    31261 ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt
    31321 tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc
    31381 agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaaagcc
    31441 cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta cagttcagcc
    31501 gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt tgcgcctttc
    31561 tcttccgctt ttttgttcag catttcacgc atatccattg gcgaagacgc cacaccactt
    31621 acggatacgg tcccgattgc ttcacgattt tgtgcttgtg cagtatcaat ggagtcggca
    31681 gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagctacagt ggttttgatt
    31741 ttcatgattt ttacctcgac ataatctttt agctgggcct ttgtttcgtg atccatatca
    31801 caaaatcaag catacactaa ttattgaact aattaatacc acactaactg tttcagttga
    31861 tactaagtgt taaaaatgtg aattttaata acaaaaaaga attaaaaaca cggttattta
    31921 ttgttaattc tagtattttc aattagataa tgaattttac ataatgtgct ttttttaccg
    31981 tatgtattca ttgtgtgaat gatatgtcgc ggtaaaacgc actattcgtt aaattaatcg
    32041 caggggaaag cagggggttg agaggaataa gcaacatttt ccccgccgcc agaagcgacg
    32101 gggcagagat taaagctcct ggtcgaacag ctctaaaatc gcttcgtaca ggtctttgac
    32161 ggtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg ccagcgatgg tgcccagaat
    32221 accttctgct ttgcccagtg agtccagcag gcgagcaatt aactgcgccg cgccagggct
    32281 ggtatgaatc acgacaactg catcgttgta gtcgatatcc agcaccagat tcttcaatgg
    32341 actggaggtg gttggtacac ccagttcagc tggcaggcag taaaccattt ccattttggc
    32401 attgcgtgta cgtacagcac caaacttggt caacatccgc gagactttag actgattaat
    32461 attgtcaaag ccttgctcct gcaacgcggc gacgatttcg ccctgggagc taaatttctc
    32521 ttctttaagt aatgctttaa atgctttaac tagttcttct tgcttagccg agcttcgcat
    32581 aagtcacccg atatggtggt tgatacaaca ttattgtgca tacagatgaa tttttatgca
    32641 aacagtcagc cctgaagaag gctgaaataa tgttatgaaa gagcgggatt ttatcaaatt
    32701 tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt ctgtttaaaa gtaaattaat
    32761 tgttatcaaa ttgatgttgt tttggctgaa cggtagggta tattgtcacc acctgttgga
    32821 atgttgcgct aatgcataag cgactgttaa ttacgtaagt taggttcctg attacggcaa
    32881 ttaaatgcat aaacgctaaa cttgcgtgac tacacattct tgagatgtgg tcattgtaaa
    32941 cggcaatttt gtggattaag gtcgcggcag cggagcaaca tatcttagtt tatcaatata
    33001 ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc tggcggtatt ggccaggcgc
    33061 ttgcactact gttaaaaacc caactgcctt caggttcaga actctctctg tatgatatcg
    33121 ctccagtgac tcccggtgtg gctgtcgatc tgagccatat ccctactgct gtgaaaatca
    33181 aaggtttttc tggtgaagat gcgactccgg cgctggaagg cgcagatgtc gttcttatct
    33241 ctgcaggtgt agcgcgtaaa ccgggtatgg atcgttccga cctgtttaac gttaacgccg
    33301 gcatcgtgaa aaacctggta cagcaagttg cgaaaacctg cccgaaagcg tgcattggta
    33361 ttatcactaa cccggttaac accacagttg cgattgctgc tgaagtgctg aaaaaagccg
    33421 gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct ggacatcatt cgttccaaca
    33481 cttttgttgc ggaactgaaa ggcaaacagc caggcgaagt tgaagtgccg gttattggcg
    33541 gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt tcctggcgtt agttttaccg
    33601 agcaggaagt ggctgatctg accaaacgca tccagaacgc gggtactgaa gtggttgaag
    33661 cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca ggcagctgca cgttttggtc
    33721 tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt cgaatgtgcc tacgttgaag
    33781 gcgatggtca gtacgcccgt ttcttctctc aaccgctgct gctgggtaaa aacggcgtgg
    33841 aagagcgtaa atctatcggt accctgagcg catttgaaca gaacgcgctg gaaggtatgc
    33901 tggatacact caagaaagat atcgccttgg gcgaagagtt cgttaataag taattgatta
    33961 gcgaataata aaaaaccgga gcacagac
//
LOCUS       DAJTXQ010000035        33975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000035 DAJTXQ010000000
VERSION     DAJTXQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.035"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..246)
                     /locus_tag="OO515_001568"
     CDS             complement(55..246)
                     /locus_tag="OO515_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575522.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(243..665)
                     /locus_tag="OO515_001569"
     CDS             complement(243..665)
                     /locus_tag="OO515_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCR6575523.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(712..1137)
                     /locus_tag="OO515_001570"
     CDS             complement(712..1137)
                     /locus_tag="OO515_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6575524.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(1554..2324)
                     /locus_tag="OO515_001571"
     CDS             complement(1554..2324)
                     /locus_tag="OO515_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575525.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(2375..2809)
                     /locus_tag="OO515_001572"
     CDS             complement(2375..2809)
                     /locus_tag="OO515_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCR6575526.1"
                     /translation="MYGTCETLCRELAAKYPGNTPLMLLIWSPEEIQALADGMEISLT
                     DHEIRTVLAHLEDIPEDQRMESGISSAAAMEIISNVSENRLVTVSAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            complement(2822..3043)
                     /locus_tag="OO515_001573"
     CDS             complement(2822..3043)
                     /locus_tag="OO515_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575527.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            complement(3044..3727)
                     /locus_tag="OO515_001574"
     CDS             complement(3044..3727)
                     /locus_tag="OO515_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6575528.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQKPLPDVLGWKYSGNHHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3803..4108)
                     /locus_tag="OO515_001575"
     CDS             complement(3803..4108)
                     /locus_tag="OO515_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575529.1"
                     /translation="MTETGGKPPVSFPTKDVAVLLFLLRRLTRRGINAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(4112..5014)
                     /locus_tag="OO515_001576"
     CDS             complement(4112..5014)
                     /locus_tag="OO515_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6575530.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQDGVLLDEQNCLMLHKLWLQSG
                     TGQCRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            5432..5680
                     /locus_tag="OO515_001577"
     CDS             5432..5680
                     /locus_tag="OO515_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6575531.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5677..6114
                     /gene="umuD"
                     /locus_tag="OO515_001578"
     CDS             5677..6114
                     /gene="umuD"
                     /locus_tag="OO515_001578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6575532.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            6114..7388
                     /gene="umuC"
                     /locus_tag="OO515_001579"
     CDS             6114..7388
                     /gene="umuC"
                     /locus_tag="OO515_001579"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HCR6575533.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGGNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGELQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(7390..7806)
                     /locus_tag="OO515_001580"
     CDS             complement(7390..7806)
                     /locus_tag="OO515_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6575534.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(7799..8779)
                     /locus_tag="OO515_001581"
     CDS             complement(7799..8779)
                     /locus_tag="OO515_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6575535.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(9193..9501)
                     /locus_tag="OO515_001582"
     CDS             complement(9193..9501)
                     /locus_tag="OO515_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HCR6575536.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(9588..10232)
                     /locus_tag="OO515_001583"
     CDS             complement(9588..10232)
                     /locus_tag="OO515_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR6575537.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(10412..11191)
                     /locus_tag="OO515_001584"
     CDS             complement(10412..11191)
                     /locus_tag="OO515_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCR6575538.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(11193..11606)
                     /locus_tag="OO515_001585"
     CDS             complement(11193..11606)
                     /locus_tag="OO515_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575539.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVEKLPDVSPWSEH"
     gene            12174..12428
                     /locus_tag="OO515_001586"
     CDS             12174..12428
                     /locus_tag="OO515_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575540.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDSQSARCLNELRASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            12428..13018
                     /locus_tag="OO515_001587"
     CDS             12428..13018
                     /locus_tag="OO515_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575541.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            13231..14283
                     /locus_tag="OO515_001588"
     CDS             13231..14283
                     /locus_tag="OO515_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575542.1"
                     /translation="MKDTITPLNYTKEAIKDLSVMEKGLRSNAPLTSYIMRQVIDMLD
                     SSVKFILPNCCDIIEPEEYRQTHFDLARLPYPVVTFEIPWFKDNVETQIGDFNISPSS
                     RRIALCWEARQSFEPIPGCNSILNTYIDGGVFILPVSWSDDLKIWILGVGGMFFPYNN
                     KLTKYEPDRTLPASRLVIDTLKENGVAKYNAAHFKAEPFITSMEFKDDLIKQVGSIER
                     LYAQIIMDTRDELQAFIQACSVLNCENVCPVTLSTKPERKFINGRKVQPPEKNKRPSY
                     TYKVLQLSETKVQSNHTGTGKSGGTKRMHLRRGHIRRKNNKLIWIRPAMINANSRAGI
                     VDKDYQINIRKEENKP"
     gene            14459..15061
                     /locus_tag="OO515_001589"
     CDS             14459..15061
                     /locus_tag="OO515_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCR6575543.1"
                     /translation="MGKYQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            15117..15611
                     /locus_tag="OO515_001590"
     CDS             15117..15611
                     /locus_tag="OO515_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575544.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIIY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNNDTPKKSTVPNEDK
                     KGIR"
     gene            15611..15877
                     /locus_tag="OO515_001591"
     CDS             15611..15877
                     /locus_tag="OO515_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575545.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNKANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(16069..16350)
                     /locus_tag="OO515_001592"
     CDS             complement(16069..16350)
                     /locus_tag="OO515_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575546.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(16456..16731)
                     /locus_tag="OO515_001593"
     CDS             complement(16456..16731)
                     /locus_tag="OO515_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6575547.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(16731..17015)
                     /locus_tag="OO515_001594"
     CDS             complement(16731..17015)
                     /locus_tag="OO515_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6575548.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(17919..18950)
                     /locus_tag="OO515_001595"
     CDS             complement(17919..18950)
                     /locus_tag="OO515_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6575549.1"
                     /translation="MAGLKNTPYNAVHWCQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPKLEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            19923..20186
                     /locus_tag="OO515_001596"
     CDS             19923..20186
                     /locus_tag="OO515_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6575550.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            20104..20391
                     /locus_tag="OO515_001597"
     CDS             20104..20391
                     /locus_tag="OO515_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HCR6575551.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            20833..21366
                     /locus_tag="OO515_001598"
     CDS             20833..21366
                     /locus_tag="OO515_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCR6575552.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            21620..22303
                     /locus_tag="OO515_001599"
     CDS             21620..22303
                     /locus_tag="OO515_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HCR6575553.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFSSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            22475..22729
                     /locus_tag="OO515_001600"
     CDS             22475..22729
                     /locus_tag="OO515_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCR6575554.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            23481..24548
                     /gene="pilL"
                     /locus_tag="OO515_001601"
     CDS             23481..24548
                     /gene="pilL"
                     /locus_tag="OO515_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HCR6575555.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSATAPAQDTATTISPAAMVTTSTSPESSTTANSQN
                     KPRNPFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            24548..24985
                     /gene="pilM"
                     /locus_tag="OO515_001602"
     CDS             24548..24985
                     /gene="pilM"
                     /locus_tag="OO515_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCR6575556.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQQMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            24999..26681
                     /locus_tag="OO515_001603"
     CDS             24999..26681
                     /locus_tag="OO515_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCR6575557.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            26674..27969
                     /gene="pilO2"
                     /locus_tag="OO515_001604"
     CDS             26674..27969
                     /gene="pilO2"
                     /locus_tag="OO515_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCR6575558.1"
                     /translation="MADENINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGEISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTA
                     EEIAARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIQEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            27956..28408
                     /gene="pilP"
                     /locus_tag="OO515_001605"
     CDS             27956..28408
                     /gene="pilP"
                     /locus_tag="OO515_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCR6575559.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVNLSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            28419..29972
                     /gene="tadA"
                     /locus_tag="OO515_001606"
     CDS             28419..29972
                     /gene="tadA"
                     /locus_tag="OO515_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR6575560.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDITPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     VMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSED
                     DRAYLERHCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDA"
     gene            29985..31070
                     /locus_tag="OO515_001607"
     CDS             29985..31070
                     /locus_tag="OO515_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCR6575561.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPVLSKI
                     SSPDSWSGALGFLYGLSLFVDNYGAICAVLFAVVTGVISWSLKNWKSRSLADNIMPWS
                     IYKDIQGAAFLLNMAALLKAKMTTLNSLNVLQEFASPWLSTRLDSIIYRVRQGDHLGL
                     ALRQCGYHFPSRESANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLI
                     FLVMSLLLLVMAVVDIQSIGDNSMGSI"
     gene            31087..31701
                     /locus_tag="OO515_001608"
     CDS             31087..31701
                     /locus_tag="OO515_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HCR6575562.1"
                     /translation="MLIQNINANVLKKNEKETSHDKGWGIMEQGAIALVVIIVLALVF
                     GGLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGAKMTGALIQMGGVPKTM
                     TVRGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTG
                     LTNGITLNSTAHSDGKVTTEEASAQCTADNGSTGTNKLIFTING"
     gene            31711..32271
                     /locus_tag="OO515_001609"
     CDS             31711..32271
                     /locus_tag="OO515_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6575563.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIK
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKVWRIYRDMKGICLPGQGGRQCRQS"
     gene            32256..32912
                     /locus_tag="OO515_001610"
     CDS             32256..32912
                     /locus_tag="OO515_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6575564.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWY
                     KTHTAVLSGIWLYSIAVALIILSSSPLIYRIHAVLILSFMLQMAVTDALTGLLPGTFT
                     RRFLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI
                     "
     gene            32900..>33975
                     /gene="pilV"
                     /locus_tag="OO515_001611"
     CDS             32900..>33975
                     /gene="pilV"
                     /locus_tag="OO515_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCR6575565.1"
                     /translation="MGNLMKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGW
                     QAEARLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSE
                     GQRLQAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATG
                     ALRSWSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAI
                     DMGSNNLNNIGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGW
                     LITRNSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQ
                     LERTAVAGASCSPNGLVGRDNTGA"
BASE COUNT         9000 a         8499 c         8122 g         8354 t
ORIGIN      
        1 ctgccagggg ttttcgcgcg ggctgacagg cggcctccac cgcctgcccc tgtatcacct
       61 gtttctgctg ccctgtaaga tgtagtgacc gcccaggaaa accgcctgct gattcgccac
      121 aaaatcgcag atttcatcaa caatctgcgc ggtggtgagc ggtttctgcc ccatttcacg
      181 acgccgcgca ttaatcacgg ccctgtaatt acgcagaatt tcgcgggttt ctgtgctgat
      241 attcatgaca tcgtctcccc gtccagcgcc tgtaacacaa cgtccattgc ttcgcgttcc
      301 tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata caggcgcttc
      361 atttcactgc ctgccagcgc catcactttt tccagcatca ccgccgggac tgtcacatga
      421 cggctggcgc gcttttcttc catcagttca cgaacaacgt cgtgaacaac gctgacatcc
      481 gcaccatgct caaaggcatc gtccagccgc tgcatgacgg tttccagctc atcgtctgtc
      541 agactccagt cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaacg
      601 cattcactgt ccggcacgtc tgtatccagc acttcgcgga taatttcttt aacagtacag
      661 tacatgttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag
      721 agccgcgtaa atcgcgttgc attcggggtg aaattcgatg tgacgccaca actgcgcatc
      781 gcgcatccgg taaagctggg tcagtcctgc atccccgctg tcgtggtaca gccacaactg
      841 gcggttaatc acaagggagg taaggatgat gcctgcggca tcagcactca cggtacggtc
      901 aaaccagttt gcaccgttca ccatgtggaa acggtcgcca tccggcatca tgtaaccgcc
      961 accgtccggc aggcggatga agtgccagaa gcctccctga tattccggca tcattttttg
     1021 cgccagcgcg tacacatacg cctcggcata cagaaaatcg ttgccaaaca gatacggcag
     1081 aaaggagaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat attgcatgat
     1141 gttttcctcc gttgttcaca tattccgcga caccgggcgg tgccgtgcct ccataaaagc
     1201 cgggcgaaca ggcggggatg caggggcgca agggaagccg cagcctgacg cacacggctg
     1261 acgggagttg cagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc
     1321 agacgggaag gagggaccgg gtgaccgcag cggcgaagcg tacccttgca tcaacgccag
     1381 cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggccac ataactgaaa
     1441 aaccacaagg ccatgccccg catggccggg taaagcggag ccggcggcga cttcctttgt
     1501 gctctgtccg gggaccggca ggaaaaaggc ggggatttac cccgccagtg tcatcatttc
     1561 cggatttttg ccagggcatc cgccatgctg cggatttcgg tccgggcttc ccggaccacc
     1621 tcccgcagaa caccacggtc aaaccagcgg cggggcggtt catcgggtat acgcagcaca
     1681 tcgcaggcca gcccactgga caggatatgc tcaatacaat cgctgaccag gtacatttcc
     1741 accgacgaaa aacgtgctcc ccggttcacg atggcatcga gcaccacggg caggttttcg
     1801 tcatcttcat cgagccagca ggggtgatcg ctttccttca cgaccaggcg aaagcggcgc
     1861 gtctggcggg tcagcaacgt ctgaccggtg gtggtcagcg ccgcacggat acgccgcgca
     1921 tcttctgcca cctggtaaca gatgcttcgt accgcatcgt gcaccgcatc aagaacagcc
     1981 ctgatttcgt cttcggtcag ccattctccg gcatacggga aagacacctg cgttccgtct
     2041 gccggaagta ccggcagcga ctgccactga ctgccggaca gtaccagttc cgccccggtt
     2101 ccggtcagtc tctgaatggc ggaggccagg agcggtggca gtacgccgcc gtgacggttg
     2161 ataagtaacg tcgccagccg gggcgcatcc agccgcacgt aaatgctgat ccagcccgga
     2221 tagttcggat ttcgctggtc aacatccgtc tcttccggcc agccctgcgc cagcagcagg
     2281 ttacgggcgt gacgacaaac cagtgcattc agcgttttgt tcatgattaa gcgttctccc
     2341 tgtatcgccg cccgcagggc aggcggcgat aacatcagtt gttttcgtgt gtgttgtttt
     2401 tcagcagggt gcgggcctga ttaatcactg cctgacggcg ggtaacacat tccgggacgg
     2461 caaggccgtt atcacgggcg gcccattcac gtttccacag tgcctgttca gcggtctgaa
     2521 tcagggacgc cagcagttca gcagagacgg tcaccaggcg gttttcgctc acgttgctga
     2581 taatctccat cgcagcggcg gaagaaatac cggactccat ccgctggtct tccgggatgt
     2641 cctccaggtg cgccaggaca gtcctgattt catgatcggt cagagaaatt tccataccgt
     2701 cggccagtgc ctgaatctct tccggggacc agataagcag catcagcggc gtgtttcccg
     2761 gatactttgc tgccagctca cggcagagcg tttcgcatgt tccgtacatt tcgtctcctc
     2821 cttaatcttt gaagcacgca tccagttcaa gaatttcggt atatgcctcc atcaggaggc
     2881 ggtttgcgcg aaacaacgtc tgacgcacca gacgttccgg cagggtgtca gcattaaagc
     2941 tgtaccgccg ctccatctca ttcatggttg tacgcaggtc gcacatggca ttggccacct
     3001 ccgccacttc ggcgcgaagt ttttcgtgtc cggcatagtt catttacgca gcctccggca
     3061 taaaccagtc atcattcgcg gccccctgct gcatggcccg ttgtacggca gccagtcgtt
     3121 gctgtccggc acggtgatac tgctcaagca gctcgatacc gatataccgg cgtccggact
     3181 ggagggcggc aacgcaggtt gagccgctgc ctgcaaacgg gtccagcaca attgcgttcg
     3241 ggtgtgtgaa gctctcaatc agcggttgca ggctggtaac aggcttttcc gtcgggtgat
     3301 gatgattgcc tgaatatttc cagcccagca cgtccggcag cggtttttgt ggcagacgtg
     3361 gacggccttt tgccaggatg taggcgcatt cgtggcgata gcccacatat gcggccttcg
     3421 atgtgtagtt tttggtgaag accaggtgac caacaacgct gaatcccgca tttttccagg
     3481 cggccataaa gcgatcgacg cggttccagc cgtagaagct caccattaac gcgtcttttt
     3541 tcagcacgcg gaacatttca ttacaggccg gttgcagcca ctcatcggtt ttatcgccgg
     3601 cgatggtgcg cccggaacgg tcacggaaac cgacgagata tggagggtcg gtgaggatga
     3661 aatcaatggc gttatcagga atacgggcca taacatcgat gcagttaccg aggacaaaac
     3721 gggacatacg aaactccttc tcttacagag ggcaccggga gggcacccct gtggtgcccg
     3781 ttccccgctg cctgtgggct gttcaccgcg tccggtaacg ggtcaggccg accgtggaat
     3841 atcgcagccg tcgtcttttt gacggcgaga atatgccgcg aaagcggctt gaagggcgtt
     3901 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga tgccagccgg gagcggtaaa
     3961 agcgcccgtc ccgtgcccgg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     4021 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     4081 cgggcggttt cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     4141 ctatccacgc aggtccggtg acttccggca gctcatcgtc atctgtcggg gaagaccatt
     4201 ccagttcccc ccacagcaca tcaacgagct ctccgcgctc atacaactgc cagccacaga
     4261 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     4321 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgaagt
     4381 tgtctccctg actactgata ttcacctcat accccacagg ccacttcacg ccatactgtt
     4441 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccgtta aagccattca
     4501 cttcaacatc caggcgggtg ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4561 gcagtacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ctccagaagc
     4621 cacaccagtc acctcttttt gcggtgaaaa gtgcggtgat ggtatccctg acatcgtccg
     4681 gtaatccttc ccagcgacac tgtccagtac cgctctgcag ccagagttta tgcagcatca
     4741 ggcagttttg ctcatccagc agtacaccgt cctgcaggta tgtcagccag cgggtgaacg
     4801 cgatattctc cggcgataca acgccacgcc cggcagccgt cagtccgggg catggttcaa
     4861 accgcacatc ttcagtggtc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4921 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4981 cagcaatctg tgccggttca ccagaaaaat gcatacgatt tgagcaccag tttggcatgg
     5041 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     5101 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaacg acgaaacgtg
     5161 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     5221 ccggcacggc ctgggtaacc aggtatttta ttcacagaac cgtgcgcaaa atgctgtgga
     5281 taagctggac acagcagtcc acagcaggca taccgccaca ccctcagaga attgagcgtt
     5341 ttttagtcac aataatgaca tttgactgtg tgtggattca gttattagaa tactgtatat
     5401 gcatacagca atagaaaagg gagatgagaa catgatccgc attgaaattc ttttcgaccg
     5461 ccagagcaca aaaaacctca aatcaggcac acttcaggcg ctacagaatg aaatcgaaca
     5521 acgtctgaaa ccacactacc cggaaatctg gctgcgtata gaccagggca gcgccccgtc
     5581 tgtatctgtc acaggagccc gcaacgacaa ggataaagaa cgtatcctgt ctctgctgga
     5641 agaaatctgg caggacgaca gctggctgcc tgcagcatga gtaccgttta tcaccgtccg
     5701 gctgaccctt caggcgatga cagttacgtg cgcccgttgt ttgccgatcg ttgccaggcc
     5761 ggttttcctt cgcctgccac tgattatgct gagcaggaac tggatctgaa cagctattgc
     5821 atcagcagac ctgcagccac cttctttctg cgcgccagcg gtgaatcgat gaaccaggct
     5881 ggcgtgcaga atggtgatct gctggtagtg gacagggccg aaaaaccaca acacggggac
     5941 atcgttatcg ctgagatcga cggtgagttc accgtcaaac gactgctgtt gcgcccacgc
     6001 ccggcactgg agccggtttc agacagcccg gagttccgca cactgtatcc ggaaaacatc
     6061 tgtatttttg gtgttgtcac tcacgtgata cacaggacgc gggagttacg ctgatgtttg
     6121 cactggctga tatcaacagt ttctacgcct catgtgaaaa agttttccgc ccggaccttc
     6181 gcaacgaacc ggtcatcgta ctcagcaata acgatggctg tgtgatcgcg cgcagcccgg
     6241 aggcaaaagc ccttggcatc agaatggggc agccctggtt tcaggtgaga caaatgcgcc
     6301 tggagaagaa aatacatgta ttttccagca attatgcgct gtaccacagc atgagccaac
     6361 gggttatggc tgttctggag tcgctttctc ccgcagttga gccctactca attgatgaaa
     6421 tgttcattga tttgcggggg gggaaccgtt gcatctctcc ggaggttttt ggtcatcagc
     6481 tcagggaaca ggtaaagagc tggacaggac tcaccatggg ggtgggcatt gcgcctacaa
     6541 aaacgctggc taaaagtgca cagtgggcaa caaagcaatg gccacagttt tccggtgtgg
     6601 tcgcgctgac ggcagaaaac cgtaatcgga tcttgaagct actggggctg cagccagttg
     6661 gtgaggtctg gggagtagga cgcagactga cggaaaagct gaatgcactg ggtattaaca
     6721 cagcactgca gctggcgcag gctaacacgg cattcatccg gaaaaacttc agcgtcattc
     6781 ttgagcgtac ggtacgcgaa ctcaacggcg agtcctgcat atccctggaa gaagcaccac
     6841 cggcaaaaca gcagattgtc tgtagtcgca gttttggtga acgaatcaca gacaaagatg
     6901 ccatgcacca ggctgttgtt cagtatgcag agcgggccgc agagaaacta cgtggggagc
     6961 ttcagtattg ccggcaggtg acgacatttg tacggacatc cccctttgca gtaaaagaac
     7021 cctgttacag caatgccgct gtggaaaagc ttccattgcc cacacaggac agccgggaca
     7081 ttattgccgc cgcatgcaga gccttaaacc atgtctggcg tgaagggtac cgctatatga
     7141 aggcaggagt catgctggct gatttcacac catcgggtat agcgcagccg ggattatttg
     7201 atgaaatcca gccccgtaaa aacagtgaaa agttaatgaa aacactcgat gaactgaacc
     7261 agtcgggaaa agggaaagtg tggtttgcgg ggcgaggaac cgcccctgaa tggcaaatga
     7321 aacgggaaat gttgagtcag tgttatacaa ctaaatggcg agatattccc ctggccaggc
     7381 tgggttagtt cagtcatctc cgaacatatt ttcagcgttt cttctggtct ggtcttcacc
     7441 gtcatttctc gacagactct gctctgtaag aggagtactg caatccacag acgtatggag
     7501 accagattgt ttttccagta attcaagcaa tttcttttca accgaaaaag cacctggtat
     7561 cacgctggaa atcaccttat taatatcatc cagagtcgta ttttctgtca gtaactccga
     7621 tatcagcaaa ggaagacgtt tatctatagc tgcaaacgcc gcaccagtaa ctaacatcgc
     7681 gcgcatgaaa cgtccccgct ccttcagttt ttcatttgca atgagctcat ctgctatctt
     7741 atctcctgtc cgttcatccg ggaaaagttc aaatgagcag cgtctggatt tattttcatc
     7801 actcattagt cacaccacct ttcattttta tcattcccat gactaaatca aactgcgggg
     7861 atgagctcag gtaaaagcgc tcatcaggaa ccttagtcag gttcttcaca gcctcggcca
     7921 caatttcagc cccaccacca acacacatta catgtgtata gtcggtgaaa cggctgatga
     7981 catcattaac tcgtttaata agcaacttct gccgttcatt gataacatta atgatagact
     8041 cacgctgttc agcattataa atgcgacggg aaaggtagtc aggttcatta cggtgcacaa
     8101 tgatattgtc agcctggaat gagctcactc ttgtattagc atgtacagcc atctgttcct
     8161 taaccccgga tgtaatcagg gacacgccga tatttggatc acaccaggtt tttgtaatcc
     8221 ctgtcatttt gctacgaaca tgcgacacat caagagttgt accaccgaga tctacgatca
     8281 aaagggattc atcatcctca agccccgcaa gaacactgaa accagcggga atactttcag
     8341 gaagaacgga aacactgcga ataacaaatg catcactccc ctgcacccgt acttcacgca
     8401 tcacattttt tttctttctt tcaatgtttt gcttattttt ctgattgttg gcgtcaaggt
     8461 attcagagat cggcaatgtg acgatgacat caacaggctg cgctttcaga ccggtctgct
     8521 gtagcgcatg ctgaatggcc acaacattca catcactgta ctgatagcgt gtttcagtcg
     8581 ttacgacagc atcagcactt aatggatcaa atgaaaactt ctcgccgtcg atctcgtaat
     8641 tagccggggc agcatcatcc agtaaactaa aagaccattc cggtttaaag ctgttagggc
     8701 tgattaacgt tttcacgtca ccatcctcca accaggcaag tttaatgttg gtagaaccat
     8761 catcaataaa gattttcata accagtctcc gttgctcata aacaaataga aaaaacaaac
     8821 atgcttcata catgcaatgt atattcaatt ataatgaaaa aacggtcttt ctgaaaggac
     8881 aaatgcaaca taaaaacacg ataaattgca tgtacattgt acacacaact ttaagaccag
     8941 aggaaatcat agatacaaaa acattatgaa ggttatatgt gatacataaa tgcaatataa
     9001 aaacatcgtg gacatcatgg ttcaggttag gggaaaagga atgaaatggt gaaactgaga
     9061 ggaaaggaaa gcggtgacga atcatgtcac cataaacaga aagatgtcta atggggactc
     9121 tttaggtacg tgaagaatcc ccataacgca tttaaaagac acacatcaga tacccttaag
     9181 gtatccgata gtttattgac ctattgcacc atcacgttca agcttctctc ttatagcctc
     9241 aataatatat gagctaaaat ccagagatgt tttactggtc gctttaagtt ccgcatgagc
     9301 ttcaaaatac gacagcggta tggccttaag attcttagcc ttcgtcctgg cagccacggg
     9361 acgacctgaa acttcctctg caccttcatt tggctgagac ggggctgttg ctgttacggc
     9421 agaagaagaa gtttgtaatc tggcagaatc caccaggttt ttcagttttt cagcctgtgc
     9481 actaaatttc gctttgctca tgagataacc caggatgttt gttagatact taaaagatat
     9541 ccattaaaag cccaaaggat actatatgga tacccaatag acacacctta ctgtttgtta
     9601 ttttcaatca gatgattgat ttcttcgatc agggcaataa cttccttacc tcctgccgaa
     9661 tagcgcccat gaacagactc agtgattccc atcccagtct caatgacatc ctcaaattct
     9721 gcacgagcag aaatgcggct cttcatcagc ttcagatgtt tgaacgatgg caggattgca
     9781 tcaagcttag ggaatttctt cttgttagga ttaactttac agagatacag gtgagcagtt
     9841 atatcggtcc ccatgataga gctgatttcg tccagtactc catcaaaatg catcaaacca
     9901 atacgttcag tcagggagtc ttttgaaggg acaataacgc agtctgcaaa ggcaatcgca
     9961 gtacgggtaa gatctgagtc aaatccccca cagtcaataa gtaacgtctt atcgctgttc
    10021 ttgtagggag tcataatttc gattaactca tccactgttt tgggtacttt cacggaaatt
    10081 tcttttcctt caggtctcag ccccagaatg atagaaagcc cggtatgagt atccagatca
    10141 actgtatcag acttcatttc accaacaatg tttacggatg aagttgtctt acccacacca
    10201 cctttgggat gcgtattagc aataacaggc atagtgtatc tccagaatcc acatgtaata
    10261 cccataaagc ccccataaga cacctttcgg atatccaaga ggtactcaat agatgttttt
    10321 ttactctata ccggtcctgc atgtcaacag aatatgaaat accgataata tatccatgag
    10381 atatccgaaa gggatttaaa aaagtcagag cttatggaat tcttttgagc atagacaccg
    10441 catcggactc aggcatcgaa aactgcaccc ggtgccgtgc agccacatcc agtgcaaaca
    10501 ccttcgtgta gacctccgtt gagctgatgg acttatgccc catcagactc tgcaggacct
    10561 tcagcggtat accggcatac agcatatgca tcgcatagga atggcggaac gtatgtggcg
    10621 tgaccgggac agagaacgtc acaccatcag cggcagcggc ggcaaccgcc tccccaatcc
    10681 aggtcctgac cgttctgtcc gtcacttccc agatccgcgc tttctctgtc ctgcctgttc
    10741 gtttattacg ccgttccatg gggattttca gtgtggccac catcgtctgc agctggctga
    10801 cgtaccaggc gtcggagagc ggaaccagcc ggtgagtctg ctgaccggcg ggcattcttc
    10861 ctgccgtcct ggcggctttt tcggtccgct gcttcagagt ggccagctgc acaaacggat
    10921 acggaggcgt gagcgaaaaa tccccccgcg tcagcgccag tgcttcatta atgcgcgcac
    10981 cggtgttcca cagtgtggcc agcagcatct tgcggcgcag atccgggacg taatggagca
    11041 gggcgctcac ttcaggggcc agcaggtatt ttggcagttc atcatgaacc atcgacatct
    11101 ggcggagtgc cagcgctgcc ggataatcga tggcaacagg aagctgagcg gcatcccggg
    11161 catggtggat cggtgccggc aggtgctgca tatcagtgtt cactccacgg ggaaacgtcg
    11221 ggcagttttt ccacaacccg gtactttccc ggataacgga gctggttacc gccgcgcgtc
    11281 cagacggtga caacaaaacc cccgccctgg ttgttttcgg ggagcaggcg atcgcactgc
    11341 tcactcagca tgtggatcac gaacggcgca tcgctgccgt cttcaaatac cagttccagc
    11401 gcatcgcggc catccagcgt tcccttactg atgatgacgt actcagcccc ccgcatctcc
    11461 ctgagtaaat gttttgccgc atccgggaca agcagcctgg ctgcgccggc attccagctg
    11521 aggtagacat atccggcctg cgcctgtaca gactgccagt aatccgttcc ggcgacagcc
    11581 tgaccttcat tctcaataaa aaacatgggt gactcctttt ttcttttccg ggcagggacg
    11641 gtcatgcgat aacgtcatcc gtcatcactg cccttaccgt cacgccgtct gataacatca
    11701 tgccgttatc atcatcacca tgataacaag catccatata accacgccgt taaattaccg
    11761 gagagccgct tccgtcagat ccgactttcc tgaatatcac aactttccgg aagtgtatca
    11821 cgctgaggaa ataatcatat cgctgttttc cgcccctgcg cggcgcatca caggtcaggg
    11881 agatgacaca acatatagtg tcatcagcag ctgaatcagg caccacatgt tgtgctgacg
    11941 ggaggtggcg ttgtgtgggg ggagtgggag atccgtctcc gccggaaacc ggatctgcgc
    12001 ggcagaaaag acgcaaataa tatgataata aaaaacacac ataaaatcat atctgatatg
    12061 attttcatca ttgatgaaaa aaccatatca gatattatca taaatcaaga gcacacaaat
    12121 catgcatgat atgattttta gctgtcggaa cattatcaga tggagcatgc aggatggatg
    12181 aagaaagggt gttttcactc agctatgagc agttgacccg aatagcggaa aaaaatatcc
    12241 gtgaatgtgg gctggacagc cagagcgcca ggtgcctcaa tgaactgagg gcctcagctc
    12301 tgctgtggtt gtggtatgaa ctggccattc atggtgcgcc acagaacaat cacgcacagg
    12361 cccgggaacg catcgatacc gattatcagc gcctcaaaaa actcatctgg tcggagggtg
    12421 acagttaatg actcagagcc gccgaccgtc gccactgcag cggagagtgc tgattgtgct
    12481 ggctgccctt gatgaaaaac ggccgggacc cgtgctgacg cgggacatag aacgggtgct
    12541 ggagcgaagc ggggaagccc cggtctacgg gccaaacctg cgcgcctcct gccgtcgtct
    12601 ggaggatgcc ggctggctgc gtaccctgcg cgctccgaac ctgcagctgg cagtggagct
    12661 gacggacgcc ggccgggccg tggcacagcc tctgctgctg gcagaacagg accgcctccg
    12721 ggcagaacag cgtgcggcag aagtcgtcgt actgccgctg gtcccggcag caggtctgcc
    12781 cgcagacggc acttctgcca ctgatctggc ggtggagctg aacggcataa cgtatcaggc
    12841 ctgccgtggg gattttgtgg tgcgtctcga cggcagcacc tgtctgcagt tatggaataa
    12901 agaaggcagg gtggtacgcc tggagggcga tccgctggaa gtggcgcagt ggctgcaggc
    12961 ctgtcatgac gcaggaatag aggttcgcgt acagataaat gaaagtagcg tcccgtaaag
    13021 ggggcctgag taaagcggcg cggatggctg attatttcca agaataatcg tcagttcagg
    13081 tatcgtggat acctgtaaca gcctcctgtg tacgagctaa atccacacat cttctgccgt
    13141 agccaggaac ctactggctg agtatttctg gtggcagagc tacgtaagta gccgtacctg
    13201 tatagtaatc agatatccag agaaaacgta atgaaagata caattactcc actaaattac
    13261 actaaggaag ccataaaaga cctgtctgtc atggaaaaag gattaaggtc aaatgcacct
    13321 cttacatcat atattatgcg tcaggtcatt gatatgctgg acagtagtgt taagttcatc
    13381 ttaccgaact gctgcgatat tattgaacca gaagaatacc gccagacgca tttcgatttg
    13441 gcacgattac catatccagt tgtaacattt gaaataccat ggttcaaaga caatgtagaa
    13501 acccaaatag gtgattttaa tatatcacca tcatccagaa gaattgccct gtgctgggag
    13561 gccagacaga gttttgaacc catcccggga tgtaattcaa tccttaacac ttatattgat
    13621 ggcggagtgt ttatccttcc tgtttcatgg tctgatgatt taaaaatatg gattcttggt
    13681 gtcggtggaa tgtttttccc atataacaac aaactcacta aatacgaacc tgacaggaca
    13741 ttgcctgcat ccaggcttgt aatcgacaca ttaaaagaga atggagtagc caaatataat
    13801 gcagcacact tcaaagcaga gccttttata acaagcatgg aatttaaaga tgatttgata
    13861 aagcaggtgg gaagtattga aagattatat gcgcaaatta ttatggatac tcgcgatgaa
    13921 ctacaggctt tcatacaggc atgtagcgtg ttaaattgcg aaaatgtgtg tcctgtcaca
    13981 cttagcacta aaccagaaag aaaattcata aatggtcgta aagttcagcc acctgaaaaa
    14041 aacaagcgcc cttcatatac ttataaagta ttgcaactgt ctgaaactaa ggtgcaaagt
    14101 aatcatactg gaacaggaaa atccggtggg acgaaaagga tgcacttacg cagaggacat
    14161 attcgcagga aaaataacaa actaatatgg atcagaccag caatgattaa tgcaaacagt
    14221 agagcaggca ttgttgataa ggattaccag ataaatataa gaaaagaaga aaacaaacct
    14281 tagtaaatct tactgtgtaa gtaacaatcc gttgtatcct gctataaacg ttcctgattc
    14341 acatagcagg gtacagagtc atcttcgtaa attatcaagg gatattacat aacagaaagt
    14401 ttattaaatt ttgaactatg atattataag gaagtagaac aacatagccg aggtatttat
    14461 gggaaaatat caggaagaaa ggaaaactcc cgtcattgtg gtgaaaaaac gtcgtacttt
    14521 ttcgccaccg tccttatccg aaaaaacaga tattatcgca cctgtattta ctgaacagac
    14581 ggcagaatca gcccccgccg gaataaattc atctgccgtc gaaacacata tccccgaagc
    14641 gccagccagg aagaagaaaa agaaaagaca ccgttttcca cgtccctccc actggacccg
    14701 tgagtatact cacgagtgcg tggaaaaaat aaaggccctg tttcctcatc ttcgtgctga
    14761 aggtggtgga tttattcccc tgaaaattgg cataaacaat gatatttcgg catttctggc
    14821 agaacacccg gagacagaac tgaccatgga cgaatggctc tgtgcagtgt cctgcatcac
    14881 atcccgccgc gtatatctgc agcggacagc tgtcgccggc gtcccacgtt atggcctgga
    14941 tggtcacccc aaagggcagg tctcagactc tgaagcacag agtgccggtc gcagactggc
    15001 gacattagaa caaaagtggc tcaggatgca ggcacagcag gagaatattt ctgggcaata
    15061 aacactcctg ataacaggtg gacattacgt atcagaaaaa atgggaagag aatgttatgt
    15121 gtgattcaga agcggagaag attttattca tcctaaataa acatgaacgg gcatacagac
    15181 gcctgatccc cgtatatatt tttttagcta tagtattatg gattttacct attctcctga
    15241 ttatatacct gtatttctgt atagttgaca atgaatttct cagaatcggt ttctgtattg
    15301 catggacagc atactacttt ctttcgcttt tcaagtattt tgtaaaatac ttctttcctc
    15361 tcgatcgtga agatttgctt aacctgatgg atctcgccaa agattctcct gatgcgaaac
    15421 aggagcttct tgagcgtctt ttgtccggaa aaagaatgac cgggcgggat gaaatggata
    15481 tcaagcgcat tctaaaaaca aaggaagcac ttgataacat caggaatttt attgataaac
    15541 agcagttaac gaataatgat actccgaaga aaagcacggt cccaaatgaa gataaaaaag
    15601 gcataagatg atgatcacag gaaatggaat aaacactgta actgtcaatg gaaaagtgaa
    15661 acacatcaca gaactggatt atataacact ttgtttagag tggacaaaac tacgagagga
    15721 aaacaatcgt ctttatgaaa ttaataacaa ggcaaacaga ggatggcgtg gtttcatcct
    15781 aagattgata ggggttaacc tgccagataa aaggactgag tttacacaac gtctcttgtt
    15841 gacgcgcaaa atttcgggtt cggtcatgaa aaagtaacat attctaggct ggaacagacc
    15901 aattttgcat tcgccgtcca ggagtttgag gggaaagtag tcagtgtttt acgctcagtc
    15961 agggaaagtg tgcaatgata aatgacacga aaggaatcgt agtacatggg caccgcgatt
    16021 caatattcaa ggaaaaaaca atacagcaat gtacttgttc tgtttctatt attctggcga
    16081 tttcaataaa ggcacttgct cgcgctctgt cattttctga aactcttcat gctgcatttc
    16141 gattttcctt ctaagatcat aactcgcata cctgttcttt ttatgtatat ctatatctat
    16201 ttttgtgacg agttcaaacc acaatgttcg cactccccat gcagcgttat gaaacatttc
    16261 cgcaacggct atagagtctg tattaactgc ttcagtcatg tagtcgctaa tacgttcagc
    16321 ggtaattttc gttatttcat cgtaattcat tttatctcct acaagattat ttaatggaat
    16381 gcaaaatatc tggcctgata ataatgttcc agaattaata tctgcaggct atgttaagtt
    16441 gtccccacac acaatctacc tgttttctcg tgtgtgccac aggcgcaata cataaatagt
    16501 ctggccagta agttcgtaac ggatttcgta ctcgccagca aaaatccgtc tgacctccct
    16561 gggttcaaac tgaaataact gttcccccat acgtggatga gttaacagaa tgaccggagc
    16621 ctgtgtcagg gactgcaccg ttctggcggc cgcaggttta ctggccagca ccagaaaatc
    16681 atataaccgc gccaaatcag aaagcgcctt actggtccac ttcagttcca tcagcgtggc
    16741 accggtaacg gattgtcagt actgaggctg tccgcccagg cctgtacagc ctgatggtcg
    16801 ataacctgtc cggatgtcac atcgtccagg gcttccagcg tcaggcgatt acgctcctcc
    16861 tcctgggcaa gccatgcaga aagcgcctgt ttgataatcc agccccggga gcgttccagt
    16921 ctggcggcca tctggtcgac tttatcagcc ataggtaatg gcacatgcgc ggtaattact
    16981 tttgttgcct gtgcggtatt gtttttcatt tgcatatccg attagctgtt aatcatattt
    17041 aatcatagtg attactttca gacgaaacaa ggcaaataat gcgcgtcagc gcatgtaagg
    17101 cggcactctg ttgttacata tggcacagaa acaaaaccgc cgaagcccgg cgtcagccgg
    17161 tactgattga tagatttcac cttacccatc cccatcctgc taagccatac ccgctttcag
    17221 ccatgaaata gcttctgtgc gcggtcggag tggtcccgac aagggtttac ccgcagtcgg
    17281 ggcgtatctc cgcgttagcg ggccgtcagg gccgcttacg agcgtgtact gaggacttcc
    17341 agccagaaga ctggcagcga tgacaatgta gttacaacat tcataattaa aagcgatgtt
    17401 gttcccgccc ttcgggcggg ggcggcttcg cttttcagtt atgaggggtg ggcacggtgc
    17461 tgcgtttgtg tggtgttttt tattcctgat gttttcgcgg tggtttcaga acggtgaggt
    17521 tcggggcccg cgcgttagcg tgggaggtgc ttttagattc tgagcggcga ggccttggcc
    17581 gctgcaagcg aaggcctctt tgtatttgtt cacagtggtt tcagagataa agactttccc
    17641 tgcggcgaag caacgcaggg gattaaataa tggggttagg actatattgc aggtttgctg
    17701 tttcaggttt gcagcttttt aaagttcttt gtttgcggat ttatgtggat aactgaaaca
    17761 acgtggccat gttctgatga ctgatacgtg gttttatggg cagatatgcc cctctgaggg
    17821 gatttaaggt gattactgca gattttgggc tgagcggaat cagcgccgaa agccccggag
    17881 gggctggcga tgattcagtg ctgaaggaat ggtgctgtct agtgcggcgg tggcgcagcc
    17941 agcgtcagtt caagctgacg caactccctg atggccagtc gcttcagatg gtcatcggaa
    18001 caaaaatagg cttcatccgg cggcagacgc ttcctgagat actctgccat ctcatgaatc
    18061 tgctggtcgg caggcagttt cttcagacgt ctggcgcgct tactggctgc cgcttttgca
    18121 cgacggtgtt tcagtgcatc ctggctgcgc tgcagatacc agcgttttct ggccgcatgt
    18181 acggagatat cttcatcctc acgcagtacg ccttcccgga tgagttgctg gcggatttca
    18241 ctttcacgca accgcttctg ctgctgttcg tgaagtttca ccatgtcgac gcccagcatc
    18301 tgccagcctg ccggtgttat ccagacgtaa cgtggcagac gctggcgggt ttcacggtcc
    18361 cacagggttt cctctgaaac acccagcaca ccaaaacgta cctgttcggc cagcaaacgg
    18421 gaaaggcggg agaccgtcac ttccagtttc ggaataacct tccccttgct gtctttcggg
    18481 ctgatttctc tggccagacg ggaaacactc atgcctacgg tatgtgtgcc cgcatcactg
    18541 aagctgacca gaaccggcca gacggcatcg aggaggcgtt gtttttccgg acggaaagca
    18601 tatttgcgac cggcatacgt tcgtttctgc acgtaaaaag gatgcagaga catgtgcatg
    18661 cgcaggctct gctcgccggt caccgggtcc tttttcacca gacggttgta ggcgtgcccg
    18721 agctgcccgc tcagcgcctt atagcgctca ggacgatacc aggacggatt ttcgcatttg
    18781 ggtttggtgg agtgctcacc acgacgacgc ttcgtgcgct tcctttccgg ttcaaccagg
    18841 aaagtggtcg cccttcccgc ttcatagtct tcccagaaac ggatctgctc ttcgggtgct
    18901 aactgacacc agtgtactgc gttatatgga gtattcttca gacctgccac aagctgtcgc
    18961 ttatggcgga gcgattgtgc cgtgtattta tacactgcac agggttgaat ctctggtacg
    19021 gtttcattat actttacccg tattctgtga ggcccccact atctttcgtc gttccgccaa
    19081 gttcgtaaga aagatagtgg gggttttcct tttatggctt ccggaacatc ttcagtcttc
    19141 tgacttactg cccgtacatg ccatctctcc tgaatgcctt atggcgcatt ctgccgtccg
    19201 actttcgccg ctttgctcca cagagtgcca ccctctgcac agctaataac agccgaaata
    19261 tacccgcttt acgttaattt gaaaagcatt acaacgatct cttctcttcc agtgcctcct
    19321 cactgatacc tctaataagt taatcttgac ctgcgtcaaa aaaataaaat ctatttttaa
    19381 aagcagattt ttctgcttta gatccttctt ttggattaaa agatcgactt ttgtcctact
    19441 cacgatcatt tatcttacaa ccgatccacc taacagaata atattctgtt ttaatgcaat
    19501 taaacgatct aaaaaacctt taaaaaatca ttttgtagat cattcgatcg taaatagaat
    19561 aattcttaga tgataaaacc atttgttaca caacccgcca attgtgtaac aacagcctct
    19621 acaagagcat atttgttcat atgttcgtca gaactgcgga ttgtctggac gagggtcaga
    19681 caccgtttcg tgcatatgga ccagcatgtt cgaacgccac aaagcagaag catcacgcct
    19741 ggatcgcttc ggcgcggagc gttaagtttt gacgcttttt tgtatgtttt ttaagctgtt
    19801 agttgactcg gtgcgttata ctaagtaccc tttactggtc acaggcaaaa ctgtgatcgg
    19861 gcgtaggaac ccgcgtagtc atcgtcggcg cagaagttaa tttcttcatt tggcgtcgtt
    19921 ttttgccata ccataaagac aggaacttgt atttcttgtc gtcatctatg gtagctcagg
    19981 cggggcaggc attagcctgg ccggtttccg atgacgccgg tattcctacc cccgtctggg
    20041 ctatcaccaa tgagcgtagg aactcagtga tagcgttttt agttgtcatc ggagaccttt
    20101 gctatgtcac ctcaactgat acagaaactc ccggccatca ctctgttgga aggcatgttt
    20161 cccgagctat caacaaacca gcttaaagtc tgcgtttttt acgcaatggg tgttccttac
    20221 gacgccattg ctcagaactg tcggctttct cccgaaacgg tacgaaccta cctgaagcga
    20281 agcctgaaga acttaaatct ggaaggatat gatgcacttc gctctgctgt gctgatgcga
    20341 acgttcgttt tcatgattag caatacagct aaagaaaacg aaaaaatgtg aacaggtgtc
    20401 cccattaaag gggacacttt catggaaatt gcattttgca ctttacaggc gatcccaaat
    20461 tgataaacat aaacaccaag cacaagttac tttaataaag caaatcactt cagacggtgt
    20521 cccaattatt tgtatctggc tcatgcccga tatatggcga ccttactctg aactgacgtg
    20581 ctgatgtttt atttcgcgta tcacaatact gtgccactgt attgctgata ttagtgctta
    20641 gccgttcttt atctgtgagt tcgccaaatc accgatacag aacgtactag agtctgctgg
    20701 ttatatgcct gtcagaactg cagtttatcc agatatgggc tggacactgc ttcagacata
    20761 tgatttagca tattcgcctg acatcgcaaa atcgtccatc atggatcggt tttgcgcgga
    20821 gcgttgaact tcgtgaatat agaacacctg aataaccgga actggtatct tgcgcaatat
    20881 aatacagccg gcaaaaacag agaatcttta ttctcatggc tgaatgagca aaacgtcgta
    20941 ccatggactc cattaataac aagaaagata agacgggcag actccaggtg ctgttaccgt
    21001 gaaagaatat tcgctatttt tccagggtac ttttttattc tggctaattt tgacattcaa
    21061 ccagtatcgg cattacgtcg ccatagcgcc ttcattgatt tcgtgaagtt tggcggtgaa
    21121 ataaaacccg tcaataaaga tattgttgat ggattaatga agatttaccc ggaccctgtg
    21181 ctaaatccgg gagccaggga agagctgaat gcggcttcca gcatatggct gacaaaagca
    21241 caatatcagt atctcctcag aatggaaaat actctccagc ctgagtcgag gatatccctg
    21301 ctcctggaac tggtatcaaa tgccgaacat cacggcttta tggagcgcct tgtaaacatc
    21361 ccatagccag tatttcccgg ccttcgggcc gggctttttt caattcaaga gaatattccg
    21421 gagtgaagcc tgcacgaagc catccccacc atggactccc cctgtctttt gatggaaccg
    21481 tgtatttctt aatggcgaaa gaaataaaag gcttcacccc agagtattga ttcgttttat
    21541 caaaggccgg cccgtgccat cacgtgtggc ggagagaacg gataccggtc ttcttttcat
    21601 ctcacagtag gacattcgca tgaccacgac agaacatccc gtgcgccagt tgctctggtg
    21661 tgcactgaat gccctgaaaa cggcacagga aaaccacaat ataaccagtg aaaccggatt
    21721 acggcattac ctgcttgaat ggctttctgg tgccagcaga catcctgagt tcagtagtct
    21781 ccctggcgaa atcatggcgt taaaagcatt gaccgaaaaa gatcgcaata ttcccatcat
    21841 cggaacgctg aatacgctct ttctcagctc tgccactgtc ggggaatgtc cgttgtttcg
    21901 ttttcgggct gcgctgggta aattacagaa agcaggctgg cgcacttatg tctgcccatg
    21961 gcctgagagg gtgttcaatg aatccattga acgggcctgt tccgggaaac gccatctgct
    22021 acaactaagt cgcaccgaag agtgttttct accgacaggc gatatgaggg caccagttac
    22081 gttgcagctg atcactccac tacaccgcaa aactatgcag cttctggaaa cggcagagtt
    22141 aatattctcc gacgaaggat ttcaggtggt gagaggatac gaacatcaat tcgggatcga
    22201 acgcagagaa accctctttc acaccctatt tatcgggctg cccagcttgc cagaagatgt
    22261 ctggggtgca acacaggagc agacgactat cacattacac tgaggatgtg taatccccgg
    22321 cataccccgt ttccagtctt cgcagcctgt accgcccttc ctgccgtttc cgacattctt
    22381 tctgtcacta ctcgcttgtg ccgttttttc gcaaccggta cgtgcagtta tgacggtaca
    22441 gctgcttctt ttcccgttcc ggaaggagtc tttgatgcca cagcaacatc ccggacgact
    22501 gcaaatactg gtcgtcgacg cacactgcaa acgcaggctg ttttcaacga aaacaccgac
    22561 agaccccgat gagcttgccc gacgtttctg cacgccggat aactgtctcg tggtggtact
    22621 acgcgacaac cgatttctgt tccgactgga gcgtgctccc ggcagccact gccgctggca
    22681 caagggcagc agctcccgcc atcagcatct acaggactgg ctatcatgaa cgcaaccgaa
    22741 gcaaaatgtt tattcaatgg tgtgtgatga tcaggaactg catttttttg ttccaaagaa
    22801 ttaaacagtt aatcctcctt agtctgttat tttagtcgcc tgcctcagcc aacatgaaaa
    22861 ataataacgt accattatat ctctggttgc tttggtgtac gctttgtctg tacatcacta
    22921 ccccatggca atacttacgt tatccataca ctgacaactt atcattgcct tatatgttat
    22981 atttctgtgt ggtggttgtg atttgcattt tcccactact tttcagatgt gtacttctta
    23041 ttgtaaggtc tgcatctaaa tacgacttca cattctttgt gaagaagaaa aagggaagca
    23101 agaaatcaac tatacatctt agcccctatg ctgacacacg ttgcccgaag tcaatgagga
    23161 aacactggga tgtattgctt tctctgatat caaaagcaat taataatgat gaaatcattg
    23221 tcatgcattc acatctcttt actccagcaa gaataaataa acttataaag agactgaaat
    23281 cttatgattt gagtttttca gttattacat acccacggaa aacaagtctg tacgaaaaat
    23341 tatcaattcc ttttatatat ctcttatttc agtggtcttt cccgcatttg cattcggacg
    23401 gaatgacagt cgtcattcgt cctctccagc cgcctcacat caattaacaa actcgtatat
    23461 acgaacacgg agtctccgtt atgaaaaaaa actgtctgac acttatactg ccgtcattat
    23521 tgtccggctg tgccggaatt tccggcggca ataacgtggc accatcagat acgcctgctc
    23581 tggtttttgt tgacggacaa atcagtgaga gtgctggcgt cctggcactt acgcaacaac
    23641 gactttctcc acctaaaata actccgcccc cgagagtcag cgtgccagag acagtaaaaa
    23701 cagcacagcc agtaagcgaa tcccgaccgt taccttcacc aacaataagt cagaatttct
    23761 cccggttaat cattacaggc aagcctggta gcgcccctgt aacactatcg ccgacggccc
    23821 gtaaccggac cgtcagtcag tggataaaaa cattactacc ggaaggatgg aaattcagac
    23881 aagagaatgc agccacgccc aaactgaata ccaaactggt ttcatggtct gccaatgatc
    23941 agtggacccg ttctctgaac cggttgctgg aagaacaaca cctctggggc catctggact
    24001 gggataataa aacgttgacc gtcacaacat cggctactgc accggcacag gacacggcaa
    24061 ccactatctc gccagcagcc atggtaacca catccacttc cccggagtct tcaacgacag
    24121 caaatagcca gaacaaacca cgaaacccat tcaggggaaa cagcgtatct ccgtctacgc
    24181 ccgctgcaat cccgactccg accggctcca cagtcaagtc tatcccactc atgaccggga
    24241 cacctgtcaa accggtatct cagggtaaag agtggcgtgc gccggcaggc actacattaa
    24301 gggaaaacat tattaagtgg gcagaagaaa caaaatgcga aagcatggca tcaacgcact
    24361 ggatggtcat atggccactt tctgtaaccg attaccgtct tgatgcgcct ttgatgttcc
    24421 ggggatcatt tgaatcagcc atggagcagg tatttgagtt atatcggacc gcgcaaaaac
    24481 ctttatacgc acaggcctcc cgcatgcagt gcatcattac agtttctgat acaagggatg
    24541 gtcgctaatg ggatggctgg tcatggccgt cggcctgacc attctgatta tcactggaag
    24601 ctaccagaat cagcagatgt ctgaaaccac gaacgcccag caatatgcaa gtgcgtctgt
    24661 atgggcaagt cagatactaa tgattgctaa cagaatcaat gatatcagat acgtatcagg
    24721 ccagcaggac ggagtaattt cttcagataa actggctctc cctgtcactc cggactcccg
    24781 aattaaacat cagttacaac agggacgtct gtgggtatgg atgcctgaac aaccaggact
    24841 tgttgaaact ttgcgttcaa aaagtcgtgg ttctgcacta atagggatct tccaaaacgg
    24901 gcaactcacc tggctatcag gaactgcaac cggtctgacc cccccggcag gcattactgc
    24961 tggttctgtc gtctacgtta actaatctgg atatattcat gaaaaaatca caccagcgtt
    25021 caatgaagct ggcggtgctc ccctgcatga tcgccgtcgc tctttccatc tccggatgta
    25081 ctttcagcga aatcaataaa atgcagaaaa aagcacagga ggactcagca catgcacggg
    25141 aaaaggtatc agccctttct gcccgcaaat cacaggctct tacctggctc gataatcaat
    25201 ggataaaccc tgttccggtc gctcaggtat caagagagaa aaaacaaaca gctccggcct
    25261 gctacatcac gcaggcaaga aaaggagaga tcactctaca ggaactgggg caacgtatta
    25321 ctgccgtatg cggcatcccc gtaatcatca cccctgacgc agccaattca actcttgaag
    25381 gaggcgctac ccgccagatg actgggacac taccagcacc tgatgaaaat ggtcgtctgc
    25441 cgttaagcag cctgggcagc acaacaatga ctacctccac tcagccatta acgctgaata
    25501 acctcatgtg gcagggagat atcaatggtc ttctggattt gatggccagc cgaagcggtc
    25561 tgtactggcg catggataat ggtcggattg tattctatct gactgaaacc agaacgtatc
    25621 cacttcatat gctgaacacc aaaaccagca gcagttccag cgtcagctct ggctcaacaa
    25681 gcacaatggg ggcaacagga ggccaggata actcagcatc cggtgatgct acgtcctctc
    25741 agagcacaac cgtcggtcag gaatacgacc tgtatgaaga catccggaaa actattgaag
    25801 cgatgctgac accagaaaaa ggccgttact ggttatctgc atcgagctca acgctaacag
    25861 tcactgatac tcctgctgtc caggaagccg tcgcacgata tgtggacgaa caaaacagta
    25921 ttatgaaccg ccaggtagcc ctgaacgtac aggttctgag cgtcagcaat accagaaacg
    25981 agcagttcgg tctggactgg aaccttgttt acaaatcgct acattccgcc ggagcaacgt
    26041 tgaacaatgc aagcggagat tttacaggcg ctacatctgc aggcgtatca attctggata
    26101 cggcaacagg gaatgccgcc aaattcagcg gttccagcct tctgattaaa gcactgagtg
    26161 aacaaggcga tgtcagcgtt gtaacttcac aagaaagcac ggtcacaaac ctgacgccgg
    26221 tacctatcca gatggcagat cagacagttt acgtcgccca gtcagcaaca actacgacta
    26281 cggatgtagg agcaacaaca acattaacac cgggcatgat caccactgga ttcaatatga
    26341 ccctgctgcc tttaattcag aaaacaggca atctccagtt gcagatgaac tttaatctgt
    26401 ctgatccccc aactatccgt agctttacgt caaaagacgg aaacagttac atcgaaatgc
    26461 catataccaa actgcgttca ctgagccaga aggtcaatct gaaagaaggg caatcacttg
    26521 tcgttactgg tttcgatcag aacaatacga cgacaagtaa agccggtacg tttacgccag
    26581 caaatccatt atttggtggt tcacaaaccg ggaaaaatga acgcagcacg cttgtgatca
    26641 tcattacccc gactttcccg tcaggaggca ataatggctg atgaaaatat caacccggta
    26701 gtgcttctcg cagatcctaa agtaaatcat cgcgtgtggg ctgcctgtct taaatggact
    26761 cctgtcgtaa aaaaacagcg cgtcccctca catcataaac acaagtctca tgtaaaaccc
    26821 agacggctta cctccctgaa agtgactgta ggcagcagaa attcccgtgg agagatcagc
    26881 cgccttaccg gcacaggaat actgaccagg ccagaacgca atcattattt ttccctggca
    26941 ctggctttct gctcatgggt cagaaatgga tacggtgtct tccggtacag cgataaggag
    27001 ctcttattcc ttgcttccat taatggccag cctgccgtta tggctgactt atcaggaaat
    27061 gatgccgacg ttgcccaaaa ggtcagcctt tttctggcga tgaacgaaga gccccccgaa
    27121 aaatggcagg ttgtatcgtc tctggaacat cctgataact gggaaagtat tattacccgg
    27181 ttatcatcag cagacctccg ccgttgcaaa cttactgtcg gcaatcgcag caaattcaca
    27241 cttcctgccg tactgttttt agtagcagcc ggcgcaggca cagtattctg gatgactcag
    27301 ccagagcctg atgtaggacc gaccgccgag gagatagctg cacgggcaag attacagttt
    27361 aaaaagcccg aaccaccacc ggagctcccg catccatggg cgtcacaacc tgtaatcagt
    27421 gattttctga aagcctgcgc ggaccttcga aaaccatcac cggtcgcgct agaaggctgg
    27481 aaacttaccg gtggaacatg tactcccgag acattcacac tgatttacga acgacagcca
    27541 ggaggaacta ttgaaggctt tctggcaaga agcaaagaaa tctttaatgt tattcctgac
    27601 tttaacctta aagatggtgc acgcctggct tctgtaaccc ggccactgcc ttccctgccc
    27661 cgcagggatg aggcagtccc tgccccctca gaacaactga tgcgggtgtt cacgtggttt
    27721 cagaaaaaac agttaacgcc ggccattaat gaaatagcca tacaggaacc attaccaggt
    27781 aacgatggtg aaccagcccc tgttcagaaa tggaaagaat atcagttcag tttatcaacg
    27841 cctgtaaatc ctgatgagtt gttcccttta tttcaggata ccggcgttcg tctgagcaat
    27901 atccattttg aattaaacgg cggtactttc agttattcct cagaaggtca tatatatgcg
    27961 tccaggtaaa ttactcatta ttccatccat atttttcttc tctggttttt catttgcaac
    28021 aacacaacct cttgtcacaa taggagaact tgaagcacaa caaaaccgga atattctttt
    28081 acaggcaaaa gttcagggcg cacagctaca gaaacaactc gaagaatcag atgttaatct
    28141 gtcatcagaa actgtttcag gtttctctgg tgtgactgcc tccttgccat cagtttcaga
    28201 gcagccgaca tctaaaaaca gaaaggaatt gcccgttatt atggaaataa acggcaaaga
    28261 taaacgtctt catgccgttc ttcgcatggc tgatggacgt cagacaagcg tcaccactgg
    28321 tagccagctt ccgggtacat ccgtcacggt gaagtcgatc tcattgtctg gcgtcacgtt
    28381 aagtgatggc accaccctta ctttctgagt gatgtgatat ggacaaatat aatctgaagg
    28441 atgctctcct ttttatatcc cggggagaca cccatgaaat tctcatcgaa acaaatcagc
    28501 gaacaagacc tgacgtacag agtaatctcc aggaactgct aaagttatac cctgacatca
    28561 ccccaaaagt agtctcgctg tctgagttac aggaagccgg tcaggatgat gaaaataaag
    28621 gtccgaaaga acggattatt catctgaaag atcttgcaga tgtcagtgaa agtgagaaga
    28681 aagttctctc ctattttgaa acggccagaa aacttggtgc ttcggatatt cattttttaa
    28741 tttcagagtc tattttcaaa gttcgcatga gaatattcgg tgaactgcag actgtagatg
    28801 aagatcaacc tgcattgggt tattcacttt gcgcaacagc cattctgtca atggctgatg
    28861 tcactgaaac cagtttcttc cctcaaaggg aacaggatgc gcgcctgtcc ccacaattaa
    28921 tgcgaaaaat aggaatattt ggtgcacgat acagtcacag acctacggga gatggtctga
    28981 ttgcggttat gcgcctgata cccgatgacg gagacaaagt tcccacgttt aagcaactgg
    29041 gctttatacc ggagcaaatc cggttgctga acgtcatgct gcgccgcccg gaagggaaaa
    29101 ttgttctgtc tggtccaaca ggttcgggta aatcaacaac gttacgcagt gcctgccgtg
    29161 tatatcttga cgataatcag gggcgacacc tgctgacgat cgaagatccg ctggaaggac
    29221 agatactggg ggcaacacaa accccgatta tctgtgacaa atccgacgaa gaggccgtca
    29281 aactggcatg gagtcgtgct atttcatcgg caatgcgact tgaccccgat gccatcatgg
    29341 aaggggaaat gcgcgatctt gtctcgatga tgtcaaccac ctacgcggct cagaccggtc
    29401 acatcgttct gacgacatta catacaaact cagctctggg tataccggag cgaatgatta
    29461 cgatgggtat gaatgccgat cttatttgtg atgcgcaatt actgattggg atgataagcc
    29521 agcgccttgt accaacgctg tgtccctcct gccgtattcc atgggaaaag cgagtacctg
    29581 aacttagcga agacgaccgc gcttaccttg agcggcactg caataaagat tcactctgca
    29641 gtacagataa tatctggttc cgaaatcctc acggatgctc cgactgtaat cacgacgtaa
    29701 ttattaacgg cagaaagcgc ggtgaaatag gtaaaggcct caccggcagg accgtaattg
    29761 ctgaagtgat agaacctgat aatcgcttat ttcagattct gaaaacacgc ggcaaagttg
    29821 cagcacgtaa atactggctt gaaaacatgc atggcatcag tcgtgtggag catctgcttc
    29881 gccgtatcaa tgaaggcctg gtggaccctc ttgaagccga cagaatcatc cctctggacg
    29941 aagacgaacg cctcagcatc gatgatgcct aacaggagaa caacatgcgt gaaatgaatt
    30001 tcagtcaacg gctgagacgt ttcattgtca gaaaaacgtt cagcgccccc tacagggtgc
    30061 agttttacga agctctgcgt tttctgctgg aaaataaaca gccattaaag accgcactgg
    30121 aacagatgcg ggatgcctgg acagactttg gacgaaaatg gcaccccttt gctgaactgg
    30181 ccacagactg tattgagtct cttcgtgaaa acagtggcga aaactctctg gaatatactc
    30241 ttggtttatg ggttccccag gaagaggctg cagtaatcag tgccggaata agaagtggtt
    30301 ccattgtgga tgcattacaa tttgccacca cgcttacaga tgccaaaaag caactccatc
    30361 aggccatatg gcaaatggct atttacccgg tgggattgtt aatcatgatg acggggacat
    30421 tatatgttct taatacagag ctaatacctg tactgagcaa aataagctcc cctgattcat
    30481 ggagtggtgc tcttggtttt ctctatggat tatctttgtt tgtcgataat tacggcgcta
    30541 tatgtgccgt tctttttgca gtggtcacag gtgtaatatc atggtctctg aaaaactgga
    30601 aaagccgctc tttggctgac aatataatgc catggagtat ttataaggat atacaggggg
    30661 cagcattcct cctgaatatg gctgcacttc tgaaagccaa aatgacaacg ctcaactcac
    30721 tgaacgttct tcaggaattt gcatcaccat ggttaagtac acgccttgac agcattattt
    30781 accgcgtccg ccagggggat caccttgggc tggctttacg acagtgcggt tatcatttcc
    30841 cttccaggga gtctgccaac tttctgtctt tattacaggg agatggtgca acggagctaa
    30901 tcggcaatta tggccaacgg tggcttgtac aaacgctgga gcgtgtaaaa aaacgagcag
    30961 ctgttgtgcg tttaattatg ctgatatttc ttgtcatgtc actcctgtta ctggtcatgg
    31021 ccgttgtaga cattcagtct attggcgaca acagtatggg tagtatttaa ataaaacgag
    31081 gtatttatgt taattcaaaa tattaatgct aacgttctga agaaaaatga gaaagaaacg
    31141 tctcatgaca aaggctgggg tattatggaa cagggtgcaa tagcacttgt tgtaattatc
    31201 gtacttgccc ttgtttttgg tggattgtat atgttgcgtt cacgaaccgg tgtggcaaat
    31261 gaaagttcga acatacagac aatcatcaca agtactcagg ggctgctaaa aggtagcgat
    31321 ggatatacgt ttaccagtgg agccaaaatg acgggtgctc tcattcagat gggtggcgta
    31381 cccaagacca tgacagtgcg tggtactcca tcatcaggta cagcaacgct ctataactca
    31441 tggggcggtg ccgtaactgt tgctccggct tcaacatcag gtttcaacaa tggttttacc
    31501 gtcacctatg aaaaagtccc acaggatgcc tgtatccaga ttgccacaca aatcagtcgt
    31561 actggtttaa ctaacggtat taccctgaac agtacggctc acagtgatgg aaaagtcact
    31621 actgaagaag ccagtgcgca atgcacagct gataatggca gcaccggtac aaacaaacta
    31681 atcttcacca tcaatggcta aggagtttta atgtccgggg gtaaagtgcc cctcagactt
    31741 atttcattaa tcctgctttg ttctcttatt tatcccgccc gagcttcata catcgccccc
    31801 aaacaaatct ggactaacaa gtgggacaat tgctttgccg ccgccggcgc acgctatcaa
    31861 attgaaccat tattgttgaa ggccatttct gccggggaat cttcattgaa gccgggcgca
    31921 attaacataa ataaggacag aaaaacaggc aaagccagta gtacggatta tgggttgatg
    31981 caaataaact caacccatat tccaaagctt ataaagatgg gcgtaatcaa aaagtctgag
    32041 gatttaatta ccaaaccatg tcttaatatc catataggaa gctggatact ggcaagacat
    32101 tttcagatat gtggcgtcag ctggaattgc cttggatcat acaatgccgg attccgtaag
    32161 gacagacatg aaacaagaga gcaatatgct aataaagtat ggagaattta tcgtgacatg
    32221 aaagggatat gccttcccgg gcagggaggc agacaatgca gacaatcgta ggcataagtc
    32281 tctcccctgt ttatatcctc ccattgatgt tcatattttc cattattgga cgaactctgt
    32341 tattcagagt tcgttatttt ttatctgata cagaacatct ctggtataag acacacactg
    32401 cagttctgtc aggaatatgg ctatatagta tagctgtggc attaattatt ttatcgtcat
    32461 ccccacttat ctaccgtatt catgctgttc ttattctttc gttcatgctg caaatggcag
    32521 taacggatgc actaacaggc ctcttaccgg gtacattcac caggcgtttt ttaatcgctg
    32581 gaatattatc acaaatcaca actgatatat ggtggctccg tgcaacagaa attgtctctg
    32641 ccgcaattgt cttattctgt ctgcataaac tggttaaccg ccatcgtctt aatattggca
    32701 caggagacct gtggcttatt gcaggtatta ccgcatggtc agggctatat aacgccatat
    32761 ggagtgtgtt gctgggcgca ggaggatttg tcttatggca ttcaacatgg tgcataaaag
    32821 gacataaaga aggcccactg ggtccatggc tgtgtcttgg ccatgtcctt ttgctactgg
    32881 ataatcttta tcaaccacta tgggtaatct gatgaaaaaa tatgaccgag gctgggcatc
    32941 gctggaaatt ggcgcggctt tattgattgt tatgatcata gttgcctggg gtgcaggcat
    33001 atggcaggac tatttaaaaa ccaaaggttg gcaggctgaa gctcgtcttg tcagcaactg
    33061 ggccagtgca gcccgctctt atatagggaa aaactacaca actctccagg ccagtagtac
    33121 cacaacaacc cctgccgtta tcacgacaac catgctgaaa aataccggct ttttgtccag
    33181 cggctttact gagacaaaca gcgaggggca gcggttacag gcatatgtag ttcgaaacac
    33241 ccaaaacccg gaattactac aggcaatggt tgtatccagt ggtggcaccc cttatccagt
    33301 gaaagcactt atccagatgg ctaaggatat taccactggt cttggtggat atatccagga
    33361 cggcaaaaca gccacaggtg cattacgttc ctggtcagta gctttaagta attatggtgc
    33421 caaaagcggt aacgggcata ttgccgtact gttatcgaca gatgaactta gtggtgcagc
    33481 agaggacact gatcgtcttt acagattcca ggtcaatggt cgccctgact taaacaaaat
    33541 gcacacggcc attgatatgg gatcaaataa cctgaataac attggggcag taaatgccca
    33601 gactggtaat ttcagcggca atgtgaatgg tgtaaatggc actttcagcg gtcaggtaaa
    33661 aggcaatagc ggaaactttg acgtaaatgt caccgctggc ggtgatatca gaagtaataa
    33721 tggttggtta attactcgta acagcaaagg ctggctcaat gaaactcacg gtggcggatt
    33781 ttatatgtcc gacggatcat gggttcgaag tgtaaacaac aagggcatct ataccggcgg
    33841 tcaggtgaaa ggcggtactg ttcgtgctga tggtcgcctt tatactggtg aatacttaca
    33901 actggaaaga actgccgttg ctggcgcatc atgttcgcct aacggccttg taggccgcga
    33961 taatacaggg gcaat
//
LOCUS       DAJTXQ010000036        33237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000036 DAJTXQ010000000
VERSION     DAJTXQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.036"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..664
                     /locus_tag="OO515_001612"
     CDS             <1..664
                     /locus_tag="OO515_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6575566.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            734..1510
                     /gene="yaaA"
                     /locus_tag="OO515_001613"
     CDS             734..1510
                     /gene="yaaA"
                     /locus_tag="OO515_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6575567.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1663..1959)
                     /locus_tag="OO515_001614"
     CDS             complement(1663..1959)
                     /locus_tag="OO515_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6575568.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OO515_001615"
     CDS             complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="OO515_001615"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6575569.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OO515_001616"
     CDS             complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="OO515_001616"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6575570.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OO515_001617"
     CDS             complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="OO515_001617"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6575571.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6883..7002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OO515_001618"
     CDS             complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="OO515_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6575572.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7217..7903)
                     /locus_tag="OO515_001619"
     CDS             complement(7217..7903)
                     /locus_tag="OO515_001619"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6575573.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8303..8443)
                     /locus_tag="OO515_001620"
     CDS             complement(8303..8443)
                     /locus_tag="OO515_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575574.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8539..9255
                     /gene="arcA"
                     /locus_tag="OO515_001621"
     CDS             8539..9255
                     /gene="arcA"
                     /locus_tag="OO515_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6575575.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OO515_001622"
     CDS             complement(9315..10667)
                     /gene="creD"
                     /locus_tag="OO515_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6575576.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OO515_001623"
     CDS             complement(10725..12149)
                     /gene="creC"
                     /locus_tag="OO515_001623"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6575577.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OO515_001624"
     CDS             complement(12149..12838)
                     /gene="creB"
                     /locus_tag="OO515_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6575578.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OO515_001625"
     CDS             complement(12851..13324)
                     /gene="creA"
                     /locus_tag="OO515_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6575579.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13535..14404
                     /gene="robA"
                     /locus_tag="OO515_001626"
     CDS             13535..14404
                     /gene="robA"
                     /locus_tag="OO515_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6575580.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHNFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OO515_001627"
     CDS             complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="OO515_001627"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6575581.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15100..15621
                     /gene="yjjX"
                     /locus_tag="OO515_001628"
     CDS             15100..15621
                     /gene="yjjX"
                     /locus_tag="OO515_001628"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6575582.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OO515_001629"
     CDS             complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="OO515_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6575583.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OO515_001630"
     CDS             complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="OO515_001630"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6575584.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18270..19937
                     /gene="ettA"
                     /locus_tag="OO515_001631"
     CDS             18270..19937
                     /gene="ettA"
                     /locus_tag="OO515_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6575585.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="OO515_001632"
     CDS             complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="OO515_001632"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6575586.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21497..22879)
                     /gene="radA"
                     /locus_tag="OO515_001633"
     CDS             complement(21497..22879)
                     /gene="radA"
                     /locus_tag="OO515_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6575587.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22928..23896)
                     /gene="serB"
                     /locus_tag="OO515_001634"
     CDS             complement(22928..23896)
                     /gene="serB"
                     /locus_tag="OO515_001634"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6575588.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24002..24646
                     /locus_tag="OO515_001635"
     CDS             24002..24646
                     /locus_tag="OO515_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6575589.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24674..25690
                     /gene="lplA"
                     /locus_tag="OO515_001636"
     CDS             24674..25690
                     /gene="lplA"
                     /locus_tag="OO515_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6575590.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25722..25871
                     /locus_tag="OO515_001637"
     CDS             25722..25871
                     /locus_tag="OO515_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575591.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="OO515_001638"
     CDS             complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="OO515_001638"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6575592.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="OO515_001639"
     CDS             complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="OO515_001639"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6575593.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="OO515_001640"
     CDS             complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="OO515_001640"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6575594.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="OO515_001641"
     CDS             complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="OO515_001641"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6575595.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30706..32256
                     /locus_tag="OO515_001642"
     CDS             30706..32256
                     /locus_tag="OO515_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6575596.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32228..33091
                     /locus_tag="OO515_001643"
     CDS             32228..33091
                     /locus_tag="OO515_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6575597.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         7904 a         9121 c         8372 g         7840 t
ORIGIN      
        1 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
       61 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
      121 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
      181 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
      241 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
      301 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
      361 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
      421 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
      481 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
      541 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      601 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      661 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      721 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      781 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
      841 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      901 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
      961 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     1021 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     1081 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     1141 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     1201 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     1261 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     1321 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     1381 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     1441 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1501 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1561 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1621 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1681 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1741 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1801 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
     1861 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1921 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1981 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     2041 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
     2101 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     2161 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     2221 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     2281 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     2341 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
     2401 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     2461 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2521 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2581 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2641 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2701 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2761 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2821 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
     2881 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2941 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     3001 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     3061 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     3121 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     3181 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     3241 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     3301 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     3361 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     3421 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3481 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3541 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
     3601 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
     3661 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3721 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3781 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
     3841 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
     3901 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3961 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
     4021 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
     4081 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
     4141 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
     4201 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     4261 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
     4321 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     4381 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     4441 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4501 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4561 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
     4621 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4681 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
     4741 gcagcacagg ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga
     4801 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
     4861 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
     4921 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
     4981 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     5041 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
     5101 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
     5161 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     5221 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
     5281 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
     5341 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
     5401 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     5461 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5521 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5581 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5641 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
     5701 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
     5761 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5821 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
     5881 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
     5941 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
     6001 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     6061 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     6121 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     6181 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     6241 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
     6301 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
     6361 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
     6421 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6481 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
     6541 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
     6601 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6661 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6721 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6781 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6841 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
     6901 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
     6961 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
     7021 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
     7081 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
     7141 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
     7201 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
     7261 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
     7321 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
     7381 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
     7441 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
     7501 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
     7561 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
     7621 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
     7681 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
     7741 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
     7801 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
     7861 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
     7921 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
     7981 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
     8041 tgactgtact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
     8101 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
     8161 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
     8221 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
     8281 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
     8341 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
     8401 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
     8461 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
     8521 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
     8581 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
     8641 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
     8701 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
     8761 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
     8821 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
     8881 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
     8941 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
     9001 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
     9061 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
     9121 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     9181 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt
     9241 gatctggaag attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt
     9301 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     9361 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
     9421 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg
     9481 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     9541 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     9601 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     9661 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     9721 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     9781 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     9841 aggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     9901 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
     9961 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    10021 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    10081 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    10141 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    10201 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg
    10261 atatttggcg cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    10321 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    10381 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    10441 gttttatcct cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    10501 acaagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    10561 tcggcacgtt cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact
    10621 gcaccaaaca gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt
    10681 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    10741 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    10801 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    10861 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    10921 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    10981 gttatacgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    11041 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg
    11101 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc
    11161 acatcaacag cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    11221 gtttccacca gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc
    11281 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    11341 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    11401 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    11461 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    11521 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa
    11581 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    11641 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    11701 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac
    11761 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    11821 tttgccgaat cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg
    11881 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    11941 gcctgagcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    12001 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    12061 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    12121 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    12181 gatgagtatt aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    12241 taatgtgggt atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    12301 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    12361 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    12421 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    12481 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    12541 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    12601 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga
    12661 tatcaggcaa accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    12721 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    12781 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    12841 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    12901 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    12961 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    13021 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    13081 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg
    13141 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    13201 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    13261 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    13321 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    13381 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    13441 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    13501 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    13561 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    13621 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    13681 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    13741 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    13801 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    13861 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    13921 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    13981 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcaca attttctcgg
    14041 caacgcgccg accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    14101 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    14161 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    14221 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    14281 aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    14341 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    14401 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    14461 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    14521 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    14581 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    14641 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    14701 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    14761 cagagaatcg atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc
    14821 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    14881 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    14941 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    15001 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    15061 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg
    15121 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    15181 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc
    15241 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    15301 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    15361 gtggtgattg aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct
    15421 gcggtgattc tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat
    15481 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    15541 ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat
    15601 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    15661 acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag
    15721 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    15781 acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc
    15841 gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    15901 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    15961 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    16021 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    16081 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    16141 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa
    16201 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    16261 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    16321 ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg
    16381 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    16441 ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg
    16501 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    16561 gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg
    16621 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    16681 acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt
    16741 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    16801 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    16861 tgcggcgctt tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc
    16921 gggtagaaac cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt
    16981 ccgcgttcca gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc
    17041 atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt
    17101 cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat
    17161 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    17221 tcctgaatct gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt
    17281 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta
    17341 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt
    17401 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    17461 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    17521 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    17581 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    17641 catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag
    17701 gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    17761 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    17821 tgattcatca gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    17881 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    17941 atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    18001 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    18061 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    18121 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt
    18181 gatcgaccaa ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    18241 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    18301 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    18361 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca
    18421 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    18481 ttggttacct gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg
    18541 aagaagcggt ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt
    18601 acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga
    18661 tcattcaggc tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc
    18721 tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg
    18781 tagcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca
    18841 accacctgga tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca
    18901 ccgtggtggc gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg
    18961 aacttgaccg cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga
    19021 aagatcagcg cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga
    19081 aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc
    19141 tggcacgctt tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac
    19201 tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta
    19261 aatcctacgg tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa
    19321 tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg
    19381 gtcaggaaca gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg
    19441 ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg
    19501 ggctggatat tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct
    19561 ttaactttaa aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg
    19621 gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac
    19681 caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc
    19741 cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca
    19801 tcctggacta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg
    19861 aagagtacaa gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca
    19921 agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta
    19981 caaaccaaga aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc
    20041 gcatcaggca ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac
    20101 agccttagta tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt
    20161 aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc
    20221 gcctttgagc cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc
    20281 acgcagcgca ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg
    20341 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    20401 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    20461 aaacggtatt catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg
    20521 cagaacgcct gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc
    20581 actgcaaaat caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc
    20641 aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca
    20701 ctggtggtat tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    20761 ccaccaagga tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc
    20821 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    20881 ggatcgacca gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct
    20941 tccgaggtgt agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg
    21001 ttgctccaca catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga
    21061 atgcgaatat ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc
    21121 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    21181 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    21241 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga
    21301 aattcaagcc ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt
    21361 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    21421 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    21481 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    21541 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    21601 cccggcgaaa accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc
    21661 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    21721 cacgcaggct ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct
    21781 tcacgccgcc gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac
    21841 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    21901 gcggattcgc catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    21961 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    22021 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    22081 gctcattcac cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg
    22141 catcaccatc cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca
    22201 gcgagccatc tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga
    22261 agcgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    22321 catccgccat atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt
    22381 cggcaatcag gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    22441 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat
    22501 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc
    22561 ccgcacctgg gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta
    22621 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    22681 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    22741 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    22801 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    22861 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg
    22921 cgagcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    22981 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    23041 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    23101 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg
    23161 cgtctacgat gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat
    23221 tggctaccac ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc
    23281 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta
    23341 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    23401 tcagcgtcgc cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    23461 gttcggttac ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac
    23521 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    23581 gcgggatttt ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc
    23641 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    23701 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    23761 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    23821 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    23881 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    23941 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    24001 gatggctcgc acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct
    24061 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    24121 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    24181 tgcaccgctg atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca
    24241 gttaacggat gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    24301 cgcccgttca ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc
    24361 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    24421 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    24481 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    24541 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    24601 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    24661 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    24721 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    24781 tctctggcgc aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg
    24841 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg
    24901 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    24961 cgataaaacc atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    25021 cgaagcgtcc ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc
    25081 aggctcggcc tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa
    25141 tgccgacctt agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    25201 aggcattacc tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac
    25261 ccatgagcag gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt
    25321 agaagcggaa atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt
    25381 tgcccgtcag agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct
    25441 ggatgaacgc tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat
    25501 cacccgcgcc caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg
    25561 gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt
    25621 ggttgacttc ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc
    25681 ggtaaggtaa ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc
    25741 aggagcggga gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc
    25801 gtatcaggca taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt
    25861 ctctaaaata gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag
    25921 aaatttatgg ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata
    25981 aatgaataga ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat
    26041 aatagtaaat ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc
    26101 ccccactccg gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc
    26161 cagcagaacg gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc
    26221 ggcagtggtc tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt
    26281 cgcgccaaat tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat
    26341 gccgtatttt tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga
    26401 gaacaggtta ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg
    26461 caccatgtca aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac
    26521 tttggaatcg gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag
    26581 aactgcgcca caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc
    26641 tttggtgtag atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg
    26701 gcctttgtaa gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc
    26761 ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac
    26821 gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc
    26881 aacatgtttt caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc
    26941 attatcagaa catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag
    27001 tctggccgat atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc
    27061 cgtaaaccag taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac
    27121 aaccgtggtc agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg
    27181 gcagacggcg gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc
    27241 cccaggaaga gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt
    27301 tcatctcttt gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg
    27361 tgataccgca gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt
    27421 tttcatcaac cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac
    27481 ggttaccggt acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac
    27541 gaccgatgtt gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga
    27601 gtttatccag gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg
    27661 tatagaaaat cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg
    27721 taccggaaga gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat
    27781 ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa
    27841 ccgggacacc ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt
    27901 gcgcccatgc gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac
    27961 cggtagaacc ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg
    28021 gacctttacg accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg
    28081 tgtcagcccc gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt
    28141 ccagcaccat aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa
    28201 tgatcagatc agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg
    28261 gtgctttatc ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt
    28321 tttcgtcttt cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac
    28381 gcgccatatc agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc
    28441 cgcccattgc aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt
    28501 cggtatcagc atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct
    28561 caacgaagtc ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt
    28621 taccgttgtc cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt
    28681 tgccggagat aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac
    28741 gatactcacc cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg
    28801 aggccagtac ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag
    28861 ccacgccaac aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc
    28921 cgctacccac tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca
    28981 gaatagaggc ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt
    29041 agaaacgttt atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt
    29101 ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt
    29161 ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat
    29221 agccgccgca ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac
    29281 cagtggaatg tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg
    29341 ttcctgaatc tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga
    29401 agaaaatggt catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc
    29461 cgttgataaa gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa
    29521 taatttcttg tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg
    29581 ccggatgcgt ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc
    29641 gcagcgcatc aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt
    29701 ggcccagcgc tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg
    29761 catctgccca gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt
    29821 ccgcagtacg cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac
    29881 ggatcacttc catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg
    29941 tagaggtttt gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca
    30001 gcgcttcgtc tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg
    30061 cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca
    30121 gcgcgcggta cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg
    30181 tttctgccag cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga
    30241 ttcggatttc cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag
    30301 gatagataca gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa
    30361 ttactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac
    30421 gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc
    30481 accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac
    30541 aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc
    30601 actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa
    30661 acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat
    30721 gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag
    30781 cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct
    30841 gccgaagccc gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg
    30901 ccgtacaaac cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa
    30961 tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt
    31021 taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg
    31081 ttgcaaccat atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc
    31141 tggcgttacc tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct
    31201 gattcgccca ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg
    31261 aacctgacct ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg
    31321 aagaacatct gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt
    31381 ttcacaaaag ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt
    31441 ggcagcacgc tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat
    31501 gacttcttta cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg
    31561 cggtgtgaat tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa
    31621 gggctgatta accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg
    31681 gttaacttgc tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca
    31741 aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg
    31801 aaatatggct ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc
    31861 ggcaccacgc cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg
    31921 caaactgtcg ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc
    31981 gacgaaacca tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa
    32041 gccggaatgc gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt
    32101 tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc
    32161 acctggctgg gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg
    32221 ataagccatg aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga
    32281 cgggccaggc agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg
    32341 tcacaatccg tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc
    32401 gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca
    32461 gtgtgatacc tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag
    32521 cgtggacgaa gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt
    32581 aagcggcggc gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa
    32641 aaacgatccg caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga
    32701 aaccggctgg gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg
    32761 ggggagcgaa tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat
    32821 ctgtttgctt gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca
    32881 ggtggattat ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt
    32941 tccggttcgc ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc
    33001 gagcgccacg ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag
    33061 ccggttgata tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac
    33121 agacttgtag gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc
    33181 ggatgcggcg tgaacgcctt atccggccta cggttcggca cagacttgta ggcatga
//
LOCUS       DAJTXQ010000037        32532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000037 DAJTXQ010000000
VERSION     DAJTXQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.037"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..93
                     /locus_tag="OO515_001644"
     CDS             <1..93
                     /locus_tag="OO515_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6575598.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(135..1211)
                     /gene="glpQ"
                     /locus_tag="OO515_001645"
     CDS             complement(135..1211)
                     /gene="glpQ"
                     /locus_tag="OO515_001645"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6575599.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1216..2574)
                     /gene="glpT"
                     /locus_tag="OO515_001646"
     CDS             complement(1216..2574)
                     /gene="glpT"
                     /locus_tag="OO515_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6575600.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2847..4475
                     /gene="glpA"
                     /locus_tag="OO515_001647"
     CDS             2847..4475
                     /gene="glpA"
                     /locus_tag="OO515_001647"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6575601.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4465..5724
                     /gene="glpB"
                     /locus_tag="OO515_001648"
     CDS             4465..5724
                     /gene="glpB"
                     /locus_tag="OO515_001648"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6575602.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5721..6911
                     /gene="glpC"
                     /locus_tag="OO515_001649"
     CDS             5721..6911
                     /gene="glpC"
                     /locus_tag="OO515_001649"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6575603.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7104..8006
                     /locus_tag="OO515_001650"
     CDS             7104..8006
                     /locus_tag="OO515_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6575604.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8047..8850)
                     /gene="yfaU"
                     /locus_tag="OO515_001651"
     CDS             complement(8047..8850)
                     /gene="yfaU"
                     /locus_tag="OO515_001651"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6575605.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8868..10157)
                     /locus_tag="OO515_001652"
     CDS             complement(8868..10157)
                     /locus_tag="OO515_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575606.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10214..11418)
                     /gene="rhmD"
                     /locus_tag="OO515_001653"
                     /pseudo
     CDS             complement(10214..11418)
                     /gene="rhmD"
                     /locus_tag="OO515_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11433..12215)
                     /locus_tag="OO515_001654"
     CDS             complement(11433..12215)
                     /locus_tag="OO515_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6575607.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12435..13637)
                     /locus_tag="OO515_001655"
     CDS             complement(12435..13637)
                     /locus_tag="OO515_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6575608.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13737..14279)
                     /locus_tag="OO515_001656"
     CDS             complement(13737..14279)
                     /locus_tag="OO515_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575609.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14558..14983
                     /gene="nudI"
                     /locus_tag="OO515_001657"
     CDS             14558..14983
                     /gene="nudI"
                     /locus_tag="OO515_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6575610.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15022..15624)
                     /locus_tag="OO515_001658"
     CDS             complement(15022..15624)
                     /locus_tag="OO515_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6575611.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15932..17071
                     /gene="arnB"
                     /locus_tag="OO515_001659"
     CDS             15932..17071
                     /gene="arnB"
                     /locus_tag="OO515_001659"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6575612.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNRERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17075..18043
                     /gene="arnC"
                     /locus_tag="OO515_001660"
     CDS             17075..18043
                     /gene="arnC"
                     /locus_tag="OO515_001660"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6575613.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18043..20025
                     /gene="arnA"
                     /locus_tag="OO515_001661"
     CDS             18043..20025
                     /gene="arnA"
                     /locus_tag="OO515_001661"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6575614.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20022..20912
                     /locus_tag="OO515_001662"
     CDS             20022..20912
                     /locus_tag="OO515_001662"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6575615.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20912..22564
                     /gene="arnT"
                     /locus_tag="OO515_001663"
     CDS             20912..22564
                     /gene="arnT"
                     /locus_tag="OO515_001663"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6575616.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22561..22896
                     /gene="arnE"
                     /locus_tag="OO515_001664"
     CDS             22561..22896
                     /gene="arnE"
                     /locus_tag="OO515_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6575617.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22896..23282
                     /gene="arnF"
                     /locus_tag="OO515_001665"
     CDS             22896..23282
                     /gene="arnF"
                     /locus_tag="OO515_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6575618.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23276..23542)
                     /gene="pmrD"
                     /locus_tag="OO515_001666"
     CDS             complement(23276..23542)
                     /gene="pmrD"
                     /locus_tag="OO515_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6575619.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23586..25007)
                     /gene="menE"
                     /locus_tag="OO515_001667"
     CDS             complement(23586..25007)
                     /gene="menE"
                     /locus_tag="OO515_001667"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6575620.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25004..25966)
                     /gene="menC"
                     /locus_tag="OO515_001668"
     CDS             complement(25004..25966)
                     /gene="menC"
                     /locus_tag="OO515_001668"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6575621.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25966..26823)
                     /gene="menB"
                     /locus_tag="OO515_001669"
     CDS             complement(25966..26823)
                     /gene="menB"
                     /locus_tag="OO515_001669"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6575622.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26838..27596)
                     /gene="menH"
                     /locus_tag="OO515_001670"
     CDS             complement(26838..27596)
                     /gene="menH"
                     /locus_tag="OO515_001670"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6575623.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27593..29263)
                     /gene="menD"
                     /locus_tag="OO515_001671"
     CDS             complement(27593..29263)
                     /gene="menD"
                     /locus_tag="OO515_001671"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6575624.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29352..30647)
                     /gene="menF"
                     /locus_tag="OO515_001672"
     CDS             complement(29352..30647)
                     /gene="menF"
                     /locus_tag="OO515_001672"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6575625.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30726..31031)
                     /gene="elaB"
                     /locus_tag="OO515_001673"
     CDS             complement(30726..31031)
                     /gene="elaB"
                     /locus_tag="OO515_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6575626.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31086..31547)
                     /locus_tag="OO515_001674"
                     /pseudo
     CDS             complement(31086..31547)
                     /locus_tag="OO515_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31612..32529
                     /gene="rbn"
                     /locus_tag="OO515_001675"
     CDS             31612..32529
                     /gene="rbn"
                     /locus_tag="OO515_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6575627.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7897 a         8804 c         8139 g         7692 t
ORIGIN      
        1 tcgttgttgg ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaatgtgc
       61 atggaacaat tagtggcgat tggaaaaatt tgatatgaga caggatggcg catgaacgcc
      121 atcctgcagt agcattactc tttattaagg aattttactg ccttatcagg gaaatcagta
      181 aacagcccat ttacacccgc tttgttatac agagcatcat ataactgatt cacatcagta
      241 gtgtattcag gcagtttatc cgaccgcacg gtataaggat gcactaccag tttattctgc
      301 tgagcatctt gcaccatgcc agtgagtttg atattacccg gctgcgatgt ttcctcaatc
      361 aacatatggt aatccggccc gataccgtcg gcatattccg cgacttgctt catggcacct
      421 ggcttaaaca tccagtcata gctgtaatta acccagcttc cgtccggctg tttctgctgc
      481 gtttcattcc agtcggtata ggcaatcagc tgtaccagat tgagatccat gcccatttta
      541 ggttccagct cattcttaat acgcttcagc tcatcagcat caaaacattg caaataaact
      601 ttatcgtcct taccggtgta accatatttc ttcagcactt ccagcgtttt tgccgcaata
      661 tccttccctt cctgatgatg gaaccacggc gctttgattt ctggatagat accgatattt
      721 ttcccggtag agtgatttaa cccctgaaca aattcaatct cttcttcaaa ggtgtgcccc
      781 cggaagtcgg acttacccat tgggaaacgc cccggataag tctgtacttt tttaccgttt
      841 tcaatatcga aaccttcggt aaatttcagc gacttaattt catccagcgt gaaatctatc
      901 gcgtagtaac gaccgtcttt gcgcgcccga tccgggaaac gatcggcaac atcagtaaca
      961 cgatcgagat aatggtcatg cagaacaacc agatggtcgt ctttggtcat caccaaatcc
     1021 tgttccagat aatccgctcc ctgcgcatac gccatcgctt ttgctggcag cgtatgctcc
     1081 ggcaaatatc cactggcacc gcgatgggcg atgactattt tttcgttgct gtccgccgcc
     1141 attgcactgc ttcccatgac tatagtgctc atcatgatcg ccatgctaag gtttttcagc
     1201 ttcaatttca tgccattagc ctccgttgcg tttttgcagt aattgttcat ggcgacgttt
     1261 ttcgccaatc atcacaacaa tcaacaagat aaccgccaga atgctgccgc caatcattac
     1321 cataaagccg ccatcccagc cgaagaagtc cacggtgtag ccaacaatcg cgctcgccgc
     1381 caccgaaccg cccaggtaac caaacagccc ggtaaagccc gctgccgtac ctgccgcttt
     1441 tttcggtgcc agttccagcg catgcagacc gatcagcatc acaggaccgt agatcaggaa
     1501 gccgataaca atcatacaaa tcatatcgac ggttgggtta cctgccgggt tcatccagta
     1561 aacgatagtc gcgatggtca ccagtgtcat aaagaaaacg ccggttgccc cacggttgcc
     1621 acggaagact ttatccgaca tccagccgca cagcagagtg cccggaatac ctgcatattc
     1681 ataaaggaag taggcccagg aggatttatc cagcgcgaaa tgcttaacct ctttcagata
     1741 agtcggtgac cagtcgagga tgccgtaacg cagcagataa acgaacacgt tggcgatggc
     1801 gatataccac agcagtttgt tcggcagtac gtactgcatg aagatttgct tcgccgtcag
     1861 ctcctgttcc gctttttcgt tatagtcgtc cggataatca tttttgtact cttcgatcgg
     1921 cggcaagcca caggattgcg gggtatcgcg catcatcgca aaggcgaata atgccaccag
     1981 aatggcgcag aaagcaggca tatagagcgc cgcatgccag tcattgaacc aggccatccc
     2041 cagcaggaac agcagcggcg gaataccacc accgacgttg tgcgcacagt tccacactga
     2101 cacaatgccg ccacgttctt tctgcgacca ccagtgcacc atagtacgac cacacggcgg
     2161 ccaccccatc ccctggaacc aaccgcagag gaacaacagt acaaacatca ccgcaatgct
     2221 cgacgtcgcc catggcacaa agcccataaa caacatcact gccgccgcca gaatcaaacc
     2281 tgcgggcagg aaaacgcgcg gattcgagcg atccgatacc gaacccatga tgaatttcga
     2341 aaatccataa gcaatcgaga tccccgaaag ggcaaaacct aaatcaccgc gtgagaatcc
     2401 ctgctcaacc agataaggca tagcaagcgc aaagttctta cgaaccaaat agtaagccgc
     2461 atagccaaag aatatcccca ggaaaatttg ccagcgcaat cgacgataag tcggatcgat
     2521 ctccgcggca ggtaagcgcg ctttgtgtgg cgctggttta aaaatactca acattgatag
     2581 cctccgtggc ccgtggtctt atttatgatt aacagcctga ttcagtgaga gaacctgccg
     2641 tttcttgagt tgccgcgatg ttaagaaaac attcataaat taaatgtgaa ttgccgcaca
     2701 cattattaaa taagatttac aaaatgttca aaatgacgca tgaaatcacg cttcactttc
     2761 gaatcatgag cgaatatgcg cgaaatcaaa caattcatgt ttttactatg gctaaatggt
     2821 aaaaaacgaa cttcagaggg ataacaatga aaactcgcga ctcgcaatca agtgacgtga
     2881 ttatcattgg cggcggcgca acgggagccg ggattgcccg cgactgtgcc ctgcgcgggc
     2941 tgcgcgtgat tttggttgag cgccacgaca tcgcaaccgg tgccaccggg cgtaaccacg
     3001 gcctgctaca cagcggtgcg cgctatgcgg taaccgatgc ggaatcggcc cgcgaatgca
     3061 ttagtgaaaa ccagatcctg aaacgcattg cacgtcactg cgttgaacca accaacggcc
     3121 tgtttattac cctgccggaa gatgacctct ccttccaggc cacttttatt cgcgcctgcg
     3181 aagaagcagg gatcagcgca gaagctatag acccgcagca agcgcgcatt atcgaacctg
     3241 ccgttaaccc ggcattgatt ggcgcggtga aagttccgga tggcaccgtt gatccatttc
     3301 gtctgaccgc agcaaacatg ctggatgcca aagaacacgg tgccgttatc cttaccgctc
     3361 atgaagtcac gggactgatt cgtgaaggcg cgacggtgtg cggtgttcgt gtacgtaacc
     3421 atctcaccgg cgaaactcag gcccttcatg cgcctgtcgt ggttaatgcc gccgggatct
     3481 gggggcaaca cattgccgaa tatgccgatc tgcgcattcg catgttcccg gcgaaaggat
     3541 cgctgctgat catggatcac cgcattaacc agcatgtgat caaccgctgc cgtaaacctt
     3601 ccgacgccga tattctggtg cctggcgata ccatttcgct gattggcacc acctctttac
     3661 gtattgatta caacgagatt gacgataatc gagtgacggc agaagaggtt gatattctgc
     3721 tgcgtgaagg ggaaaaactg gcccccgtga tggcgaaaac gcgcattttg cgggcctatt
     3781 ctggcgtgcg cccgctggtt gccagcgatg acgacccgag cggacgtaac gtcagccgtg
     3841 gcatcgtgct gctcgaccat gctgaacgcg atggtctgga cggatttatc accatcaccg
     3901 gtggcaaact gatgacctat cggctgatgg ctgaatgggc taccgacgcc gtgtgccgca
     3961 aactgggcaa cacgcgcccc tgtacgaccg ccgatctggc acttcctggt tcacaggaac
     4021 ccgctgaagt taccttgcgt aaagtcatct ccctgcctgc cccgctgcgc ggttctgcgg
     4081 tttatcgtca tggcgatcgc acgcctgcct ggctgagcga aggtcgtctg caccgtagcc
     4141 tggtatgtga gtgcgaagcg gtaacagcgg gtgaagtgca gtacgcggta gaaaatttaa
     4201 acgttaatag cctgctggat ttacgccgtc gtacccgtgt ggggatgggt acctgccagg
     4261 gcgaactctg cgcctgccgc gctgccggac tgctgcaacg ttttaacgtc acgacgtccg
     4321 cgcaatctat cgagcaactt tccaccttcc ttaacgaacg ctggaaaggc gtgcaaccca
     4381 tcgcctgggg agatgcactg cgcgaaagcg aatttacccg ctgggtttat cagggactgt
     4441 gtggtctgga gaaggagcag aaagatgcgc tttgatactg tcattatggg cggcggcctc
     4501 gccggattac tctgtggcct gcaactgcaa aaacacggcc tgcgctgtgc cattgtcacg
     4561 cgtggtcaaa gcgcgctgca tttctcatcc ggttcgctgg atttgctgag ccatctgcct
     4621 gatggtcaac cggtgacaga cattcacagt ggactggaat ctttgcgtca gcaggcacca
     4681 gcccatcctt acagtcttct cgaaccacaa cgcgtgctcg atctcgcttg ccaggcgcag
     4741 gcattgatcg ctgaaagcgg tgcgcaattg cagggcagcg tggaactggc ccatcagcgc
     4801 gttaccccgc tcggcacttt gcgtgcaacc tggttaagtt cgccagaagt tcccgtctgg
     4861 ccgctacccg cgaagaagat atgtgtagtg gggataagcg ggctgatgga tttccaggcg
     4921 catcttgcgg cggcttcgtt gcgcgaactg gatctttcgg ttgaaaccgc agaaatagag
     4981 ctgccggaac tggatgtcct gcgcaataac gccaccgaat ttcgcgcggt gaatatcgcc
     5041 cgtttccttg ataatgaaga aaactggccg ctgttacttg atgcgcttat tcctgtcgcc
     5101 aatacctgcg aaatgatcct gatgcccgcc tgcttcggcc tggccgatga caaactgtgg
     5161 cgttggttga atgaaaaact accttgttca ctgatgcttt tgccaacgct gccgccttcc
     5221 gtgctgggca ttcgtctgca aaaccagtta cagcgccagt ttgtgcgcca gggtggcgtg
     5281 tggatgccgg gcgatgaagt gaaaaaagtg acctgtaaaa atggcgtagt gaatgaaatc
     5341 tggacccgca atcacgccga tattccgcta cgtccacgtt tcgcggttct cgccagcggc
     5401 agtttcttta gtggcggact ggtagcggaa cgtaacggca ttcgcgagcc gattctcggc
     5461 cttgatgtgc tacaaaccgc cacgcggggt gaatggtata agggcgattt ttttgcgccg
     5521 caaccgtggc agcagttcgg tgtaaccact gatgagacgc tacgcccgtc gcaggcaggg
     5581 caaaccattg agaacctgtt tgccatcggt tcggtgctgg gcggatttga tcccatcgcc
     5641 caaggatgcg gcggcggtgt ttgtgccgtc agtgctttac atgccgctca acagattgcc
     5701 caacgcgcag gaggccaaca atgaacgaca ccagcttcga aaactgcatt aagtgcaccg
     5761 tctgcaccac cgcctgcccg gtgagccggg tgaatcccgg ttatccaggg ccaaaacaag
     5821 ccgggccgga tggcgagcgt ctgcgtttga aagatggcgc actgtatgac gaggcgctga
     5881 aatattgcat caactgcaaa cgttgtgaag tcgcctgccc gtccgatgtg aagattggcg
     5941 atattatcca gcgcgcgcgg gcaaaatatg acaccacgcg cccgtcgctg cgtaattttg
     6001 tgttgagtca taccgacctg atgggtagcg tttccacgcc gttcgcacca atcgtcaaca
     6061 ccgctacctc gctgaaaccg gtgcggcagc tgcttgatgc ggcgttaaaa atcgatcatc
     6121 gtcgcacgct accgaaatac tccttcggca cgttccgtcg ctggtatcgc agcatagcgg
     6181 ctcagcaagc acaatataaa gaccaggtcg ctttctttca cggctgcttc gttaactaca
     6241 accatccgca gttaggtaaa gatttaatta aagtgctcaa cgcaatgggt atcggtgtac
     6301 aactgctcag caaagaaaaa tgctgcggcg taccgctaat cgccaacggc tttaccgata
     6361 aagcacgcaa acaggcaatt acgaatgtag agtcgatccg cgaagctgtg ggagtaaaag
     6421 gcattccggt gattgccacc tcctcaacct gtacatttgc cctgcgcgac gaatacccgg
     6481 aagtgctgaa tgtcgacaac aaaggcttgc gcgatcatat cgaactggca acccgctggc
     6541 tgtggcgcaa gctggacgaa ggcaaaacgt taccgctgaa accgctgccg ctgaaagtgg
     6601 tttatcacac tccgtgccat atggaaaaaa tgggctggac gctctacacc ctggagctgt
     6661 tgcgtaaaat cccggggctt gagttaacgg tgctggattc ccagtgctgc ggtattgcgg
     6721 gtacttacgg tttcaaaaaa gagaactacc ccacctcaca agccatcggc gcaccactgt
     6781 tccgccagat agaagaaagc ggcgcagatc tggtggtcac cgactgcgaa acctgtaaat
     6841 ggcagattga gatgtccaca agtcttcgct gcgaacatcc gattacgcta ctggcccagg
     6901 cgctggctta aactcctttc tgatgcccgg taagcatgtg gttaccgggc atttttgcgt
     6961 acacgattcc gtgcccaatg tatgcgttgc aacgcagtga aaattcctct gaaaacgtct
     7021 cgcaaaggct gaaactggca gatgtcaaag gcctgggata accgtaatgt cgcgtcatca
     7081 taaatatcag gtgacggaca accatgaccg aatcaacaac ctcctccccg catgatgcgg
     7141 tatttaaaac ctttatgttc acacccgaaa ccgcacggga ttttctcgaa atacatttgc
     7201 cagaaccact gcgcaagctt tgcaacctgc aaaccttacg cctggaaccc actagtttta
     7261 ttgaaaaaag tttacgcgct tactactcgg atgttttgtg gtccgtggaa accagcgacg
     7321 gtgacggcta tatctactgc gtgattgaac atcaaagctc tgcagaaaag aatatggctt
     7381 ttcggctaat gcgctatgcc actgccgcca tgcagcgtca cctggataaa ggctatgaca
     7441 gagttccgct ggtggtgcca ttgctgtttt atcatggcga aacatcgccc tacccgtact
     7501 cacttaactg gctggatgag tttgacgatc cgcaacttgc ccggcagttg tacaccgaag
     7561 cttttccgtt ggtggatatc accatcgtac ctgacgatga gatcatgcaa catcggcgta
     7621 tagctctgct tgaactgatt caaaagcata ttcgcgaccg cgatttaatc ggtatggtcg
     7681 acaggatcac cacgcttttg gttaaaggct tcactaatga cagccagcta caaacactgt
     7741 ttaattatct gctgcaatgc ggcgatacct cccgtttcac ccgttttatt gaggagattt
     7801 ccgaacgttc accactacaa aaggagagat tgatgactat tgctgagcgg ctacggcagg
     7861 aaggacacca aattggctgg cagaaaggta tgcatgaaca agccattaaa attgctttgc
     7921 gcatgctgga acagggcatt gatcgtgacc aggtgctcgc ggccacccag ctaagcgaag
     7981 ccgatctggc agcgaataac cactaattaa cacaggccca cagccgatcc ccatgggcct
     8041 ttgatatcaa taactacctt ttatgcgtgg cccatttttg cctgatttaa acatcgccag
     8101 tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg ccaacagcga caaagttcgc
     8161 tccccacgcc aggcattgct gcgccatatc aggagccaca gccagaaaac cagccgcttt
     8221 acccgcagca cggatccgcc gaatgctggt ttcaataatt cgctgcactt ccgggtgccc
     8281 ggcgttatcc gggtagccca acgacgcaga aagatccgct ggtccaataa acacgccatc
     8341 aatcccttcg acgtcgagga tctcgtccag gttatccagt gccgttttac tttccacctg
     8401 cactaacaga caaagcgaat cgttgacttg cgccatgtaa ttctcaatgc gtccccagcg
     8461 cgcagcccgt gccacactgg ccccaacacc acgctcaccg tagggaggat agcgcgtggc
     8521 agacgccacc tgacgagcct gatcggcagt atcgaccatc gggatcaata gagtttgcgc
     8581 gccaatatcc agaacttgtt taatcagcga tttactgcct tccaccggac ggatcacggg
     8641 ttggctggca tagggcgcta ccgcctgtag ctggtgataa agatcctgaa tggtgtttgg
     8701 cgcgtgctcc ccgtcaatca gcaaccagtc ataaccggaa gtagcggcaa tttctgccat
     8761 ataagaagta gtcgagctta accacagacc aatttgcact tcgcccttgc gtaaacgttc
     8821 tttaaaggga ttggttaata atgcgttcat cgtgttcctt aacctattta atgatgtgcc
     8881 acgtcggtct gcgcaacagt acgatttaca cgcagagtga aaataatcag cgaaccgatg
     8941 accgccaccg ctgccagtgt cagtaatcct gccgcatcgc tggcaaataa cgtttctgct
     9001 ttcacgcgca ggatcggggc aataaacccg cccactgcac caaacaggtt tacaaaacca
     9061 atccccgcag ccagcgccgt accggaaagc agttgtgtcg gcatcgtcca gaacaccggc
     9121 tgcacggcaa taaacccgat agctgcaaca cacagcgcta cgatcgccat tactggagaa
     9181 agcagcccgg acagaccaat accaatgccc gccgccagta atgtcagcgc tgcgacatta
     9241 cgccgttcgc cggttttatc ggaatagcgc ggaataagcc aggtcccaaa caaggccgca
     9301 acccacggaa tggcggtgac caccgacgct gtaaagccca cttttgttcc cagcaatgcc
     9361 gcaacctggg tcggcaggaa gaaaattaat ccgtaaaccg ccacctgaat ggtcaggtag
     9421 ataatcgcta gttgccagac tcggccatta cgcagcgcat cgctcagccg agaagtcact
     9481 ttctgttgtt cttcacttgc cagttgattg ataagcaacg ttttttcttg tttactcaga
     9541 aaacgtgcct gctccggtgt gtcatcaagc caaaagaatg tgaatacccc agcaccgact
     9601 gccaacaatc cttcaatcac aaacatccag aaccagccgg gatgccccat aaatccatgc
     9661 atctccaaca gcgcgccgga aagcggtgat cccagtgtta acgccagcgg tgcgcccata
     9721 tagaacagcc ccataatgct ggcgcgatta cgctgcggaa accattgcga ggtgagataa
     9781 atcataccag ggaaaaatcc ggcctccgca gcaccaagca gagtgcgaac tatcagaaat
     9841 ttcgcttcag tatcggccca tgccatggct gccgaaagaa atccccacag cagtgttgtc
     9901 gtaccaatcc aggttctggc ccccagtttg cgcatcaaaa gattcgccgg aacacccaga
     9961 aacgcatata ccacaaagaa aatgcctgct cccagcgcat aagcttcatt actcaacccg
    10021 gtatcaatct ggtaggtctg tttggcaaaa ccgatattcg aacggtcgag aaacgccagc
    10081 acatacagcg ccaacataaa cggaattaaa cgcacacggt ttttcttcac cacggcgtca
    10141 agcaaagcgg tgctcataaa aagctcctta gaatatgtgg gcgttaccgt tgtcacagca
    10201 acgcccggag tgattagtgg ctgtaggggc gtttcaaatt gcagtcacga ttgagttcga
    10261 cgccaaaacc gggtttatca agcactgatt tatgaatacg accattcacc ggaaccggct
    10321 cattgagcag aatcgggtca aactgcggac gcatcgttga acaatccggg ctggtcatca
    10381 ggaattcgct gaatggcgta ttggtgaagg tgatcaccgc atggtgagag taaacagacg
    10441 aaccgtgcgg caccaccagt tgccccctgg atttggcgat tgcggcaatt tccaccagcg
    10501 tggttaaacc accgcaccag ccaacatccg gctgcataat gtcgataccg gtttctgaaa
    10561 gcgtacgaaa agattgcagt gtgccgtggt gctcaccgct ggtgaccatc attccgactg
    10621 gcgcgttgcg tttcagttcg cgataacctt catactgctg tggcggcagg cactcttcga
    10681 tccatttcag gttatagggc gcgcaagcgt gggccagttt ggtggcatag ttcacgtcct
    10741 gactcatcca gcagtcgagc attaaccaga aatcctcacc gcatttttca cgcatatccg
    10801 cgaccataac ggcatctttg cggatccccg catcgccatc atgtggcccc cagtgcgtcg
    10861 gcattttgcc accgataaag cccatctctt ttgccagatc cggacgcgcg cctgtggcgt
    10921 agaactgaat ctcatcacga actgcgccgc ctaaaagttt ataaaccgga agcccgacca
    10981 ctttgccgaa cagatcccac agagccagat cgacacagga aatcgtgttc atcaccaggc
    11041 caccagagcc ggagtaatac agggtggcat tgagcatttg atcatggatc agtttgatat
    11101 cactgacaca tttaccctca atgaaacggt taagatgttt ttcgacaata aaacagccca
    11161 tttcaccggc tgtcgaaaca gcgaatccgg tctggccgtt ttctgcttcg acttcaacaa
    11221 tcaacgtgcc aagaacgtta atgccaaatg actggcgtga ctgctcgtaa tcgcggtatt
    11281 tactcatcgg ggtggcaata tgatcgtcaa tccagtgatt cgccccctgg tcgtgataat
    11341 caccgcgcca gcaccttttt ctgctgtcgc accgccagta aaccaggcgc gaacctgttt
    11401 gatttttggt agggtcatga tgttctccat tgttatgagg cctgtaagtc aaagggactt
    11461 ttccatccca acagacgtga aatatccctg gcgcaggcaa tggccttgcc cgccagataa
    11521 tcacggtatt cttcattgat ttgtaagcgg gtaccgacca ccgagatcgc agcggtaagc
    11581 tcgttattgg cgttaaacac cggcgcagcg acacaacgga catcggggta atcttcgccg
    11641 ttgtcatagc tccagccctg acggcgaata cgcgccagtt cttcgtgaag ttgctgtgga
    11701 tgagtaatcg ttgtgggtgt cgcctgctcc cagaccagcc cttcgataat actttgctgt
    11761 accgctgcag gttgccaggc aagcaggcat ttacctattc cggagcgata aagcgaaagg
    11821 ctttttcctt catgggaacg cacgctgata gtggctgatg actccacttt taaaatgtaa
    11881 taagcgctgc cgttatcaat aatccccagg tgacataaca gcccggtggt atccatcagt
    11941 tgcgtcaaac gcggtcgagc cagttcccgg agatccatct tgcttaacgc atgaccagaa
    12001 agctccacca gcctggtcca caaacaaaaa ttttcctggt gatcaaggct aagaaaacgc
    12061 tgacgcctga gttcattaag cagcaaatag gccgtgcttt tagggattcc caatgtgtca
    12121 atgatcgtcg cagcactaca ggggccaatg cgggcaatca aattgagaat atcaatagcc
    12181 cgtgtcaggg cgggaacttt gcttgattcc aacatactgg actccagtct tagatactgg
    12241 aatcagtctc gttgctcagg gggagtaaaa ttgtgaagcg catcaaatcc cgctcctcgt
    12301 ccagcggact ggagaaatta cgcacaaatt tgtgaatcag tgcacggatt ttttcttatt
    12361 gagtacaaca ggttggacaa gttttgccgg agggggaatg tgccggtgca gtaaaccggc
    12421 acagagtggc ggaatcagac agataaggtc atggactcaa caacctcaat ccagccatgc
    12481 tcactggcga tatcctggcc gtttaaccaa cggcgcagca tattcagtgc catcattgcg
    12541 cacacttcct gacggatagc caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg
    12601 aaagtgccgt ctggcgtggc tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca
    12661 gccagtgcca gcccggcaaa atggttggcc cgccgttctg taatccagtg cgcagtttgc
    12721 gccagggttt cttcctgtga aggaaccact tcacacgcca gcaatggagc acctgcgcga
    12781 gaaagttgca aagccaataa accaccggtg aattgctcgc tcaacgtcag gctgaactgg
    12841 cggttttgca attcgcgact gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg
    12901 ctctgtccgg caacacgttt aacgtccagc cacagttttt ccatcgcctg ttgctcgctt
    12961 gccggtccgg tgagtttcag ttcgatgatc ggcattgagg agcgatagcc cattgttacg
    13021 cccggcggca gttgtagagt gtccaggctt tgtgccagat cgctttccga acgaccaaag
    13081 gtagttaaac gcagacaaac cggcgactgt ggtaaagaaa aacgctcgcg caggcgcggc
    13141 aggatttcgt gctcgaccat caccttaaat tctgacggta cgccgggagt aaagaacatc
    13201 aggcaacgat taagccgcac ggcaaaacca caggcggtgc ctaccgggtt attgataaat
    13261 tcagcactgg caggcagctc cgcttgttta cggttgctcg gtgccattac tcgtccacgt
    13321 tcgtggaaat agcgttccat ctctttgagc caggcttcat gcagcaccag gccttcacct
    13381 tttgctgtcg cagcggcgag tgcacttaaa tcatcactgg tcggtcccag cccgccgtta
    13441 acgatcagca catcggcgtg ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg
    13501 ttatccccca ccgtattgcg gcgagataat ggcaacccct gatgaaagaa aaaatcggcc
    13561 agccaggcag cgttagtgtc aacgatttgc ccgtgtaaca cttcatcccc ggtggataac
    13621 atttccactt ttaacatggt tatctcctgc ttttgttatc gaatcactat aacgcaaagg
    13681 gagataaggc gtagtaagaa actgaatggc gcgacagaat gccgcgccgg aagggatcag
    13741 aaactggcgt taaccccaac atacgggccg tcagccacgg cgttgtcgcg gttaccgtct
    13801 ttacccgaca gattcaggta gcgataaccc gcctcaatac tgactggatg cataatggtg
    13861 taacgcgcgc cagcattcgc ttcctcataa cttttaatac cgctggagag tgaatccgga
    13921 gagtagtaat actcgccaaa caaacggaag ctgttgccaa ttttccactg caaaccacct
    13981 cctaccgctg cggcataacc ttcatcgccg taattcgggt tggtgtaaac gccttttccg
    14041 ccaacggtcg ccattaacgg cccgagaggc agattcaacc ccagccccac gccagcgacg
    14101 tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaacg ccaggcccgt cgattcagtc
    14161 ccaaaaccga caccaatatt ggtgtattct ttacccgcct gaccgctgat gctcattgca
    14221 ttgaccgatg cagaaaccaa cagcattccg gccagacctg ctagcgcaat ttttttcatt
    14281 atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc tgcgggattg tactgcctgg
    14341 cacagaggat tcaatgagcg aaaaaaggga tctagtcact tattgtaact aaatacttct
    14401 taaataatta aacgccgaaa aaataaacac actttaccga tacacacaaa aataataata
    14461 agtgcagtta tcatcgcaaa agagattact gtcactttca catatttctg agttaccgta
    14521 ttctcatggt ttctttttat ttataaggaa tacccctgtg cgacaacgga ctattgtatg
    14581 ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa atggccgacg atcgcggcgt
    14641 tttccccggt caatgggcgc tttcgggtgg cggcgtggag tctggcgaac gaattgaaga
    14701 ggcactacgc cgcgaaattc gcgaagaact gggagaacag ctgcttttga cagaaatcac
    14761 gccgtggacc ttcagcgatg atattcgcac caagacgtat gcagatggtc gcaaggaaga
    14821 gatttatatg atttacctga tttttgactg cgtttctgcc aaccgagaag tgaaaataaa
    14881 cgaagagttt caggactacg cgtgggtaaa acctgaagat ctggtgcatt atgatttaaa
    14941 tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt taacaatgat gacagccatc
    15001 actggctgtc agattgaaaa tttagtggtt aacgaattcc ccatccagat aaactttgcc
    15061 tttttccaca tgcatgacta aaccatccag ataatcaggt ttaaatgtcg catcacgctt
    15121 atttttagca atatatgtca ggcaatgatt atgggtgaaa ataacgatat ttttatcagg
    15181 cgctttgctt tgtaagtcct taattgcact ataaatctca ttaccgcact gaagaagtcg
    15241 tttatctacc gtcagttttt tacccgcgga aaaccaggtc gccgactgaa tggtccggac
    15301 ggtattactg gaataaagat cgaaatcagg gatgtcagcg ctaaaagcgt tgcccagttc
    15361 acgggcatcc tgggtacctt taaccgtaat acctgtttta tctgacaagc attgattggt
    15421 tgaacgatcg caacgttcag catggcgaaa caaaacgaca accgggtgct gctgtgccag
    15481 tctggccaat gttttattgt cgatacgtgg caaaccattg ctactccagg cggcatgagt
    15541 acccagtccg gcaattgcag cgagcgccag taaaatgatg aaatattttt ttgacttcaa
    15601 tgatgagcgg caaaaagcta acacgtaaac tccacctata gacaagcgca accagacaat
    15661 taccgtgaaa ttgagcgaca tttctggcga taattagcag ttggtgtaat attaaaaatc
    15721 ctacgatgtc ggcaaaatgc ctcaaaattt tgccaaatgc aaagcctaaa taagaaaaaa
    15781 tataaaaatt tcaatattta cgtctaatat tagtttctta aggttaagtt aatattctat
    15841 ccttaaaatt tcgctccaaa tggcaaaata tacacaacac tctttatagc aaatataagt
    15901 ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt tttcgcgacc
    15961 agcaatgggc gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg gttggatcac
    16021 aaccggtccg aagaatcagg cgcttgagca agccttttgc cagttgacgg gaaatcagca
    16081 tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc acgctaatgg cgttggaaat
    16141 tggcaagggc gatgaagtga ttacgccttc cctgacctgg gtttcaaccc tcaatatgat
    16201 ttccttgttg ggtgcaacgc cggtaatggt ggatgtcgac cgcgatacgc tgatggtcac
    16261 gcctgaagcg atcgagtcag ccattacgcc acgcactaaa gccatcattc cggtgcatta
    16321 tgccggtgcg ccagcagata ttgacgccat tcgcgccatt ggcgaacgtt acggcatcgc
    16381 agttatcgaa gatgctgccc atgccgtcgg tacgtattac aaagggcgac atattggcgc
    16441 aaaaggtacc gctatttttt catttcatgc cattaaaaat attacctgtg cggaaggtgg
    16501 cctgattgta actgataatg aaaaccttgc ccgccagcta cggatgctga aatttcacgg
    16561 tctgggtgtc gatgcctatg acagacaaac ctggggccgt gcaccgcagg ctgaagtctt
    16621 aacaccgggc tataagtaca atctgaccga tattaacgcc gcgattgccc tgacacagtt
    16681 agcaaaatta gagcacctca acacccatcg gcgcgaaatt gcccagcaat atcagcaagc
    16741 actggcagct ctcccctttc agccattaag ccttcccgcc tggccgcacg ttcacgcctg
    16801 gcatctgttt attattcgtg tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat
    16861 ggaagcgtta aaagaaagag gcattggtac cgggttacat ttccgcgccg ctcacacaca
    16921 aaaatattat cgcgagcgtt ttcccacgtt gtcgttaccg aataccgaat ggaatagaga
    16981 acgcatctgt tctttgccgc tgttcccgga tatgactacc gccgatgccg accgtgtcat
    17041 cacagccctt cagcaactcg caggacaata agccatgttt gaaatccacc ctgttaagaa
    17101 agtctcggtg gttattcccg tttataacga gcaggaaagc ttaccggaat taatcaggcg
    17161 caccaccaca gcctgtgaat cgttggggaa agagtatgag atcctgctga ttgatgacgg
    17221 cagtagcgat aattccgcgc atatgctggt cgaagcctca caagcggaga acagccatat
    17281 tgtgtctatt ttgcttaacc gcaattacgg gcaacattca gcgattatgg cgggattcag
    17341 tcacgttact ggcgacttaa ttattaccct tgatgccgat ctccagaatc cgccagaaga
    17401 aatcccccgc ctggtggcaa aagccgatga aggttacgac gtggtaggga ctgtacgcca
    17461 gaaccgccag gacagctggt ttcgtaaaac agcttcgaag atgattaaca ggcttattca
    17521 gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca
    17581 tattgtcgat gcgatgttgc actgccatga acgtagcacc tttatcccga ttctggcgaa
    17641 tatcttcgcc cgccgtgcca ttgaaattcc agtacatcat gccgagcgtg agtttggtga
    17701 atccaaatac agttttatgc gcctgattaa tttgatgtac gacctggtga cctgccttac
    17761 cacaacgccg ctacgtatgc tgagtctgct cggcagcatt attgcgattg gaggttttag
    17821 cattgcggtg ctgctggtga ttttacgcct gaccttcgga ccacaatggg cggcagaagg
    17881 cgtctttatg ctatttgccg tgctgtttac ttttattggc gctcagttta tcggcatggg
    17941 attgctcggt gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc cccgctattt
    18001 tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt
    18061 gcctaccacg atatgggatg cctcggtatt gaagctctgc tggctgccgg ttacgaaatt
    18121 agcgccattt ttacccatac cgataatccc gctgaaaaag ccttttatgg ttcggtggct
    18181 cgtctggcgg cggaaagagg cattccagtt tatgcgccgg ataacgttaa tcatccgctg
    18241 tgggtggaac gcattgccca actctcacca gaggtgattt tctcttttta ttatcgccat
    18301 cttatttgcg acgagatttt gcagctcgct cccgcaggtg catttaatct gcacggttcg
    18361 ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaacg
    18421 gaaacgggcg ttacattgca ccgaatggta aaacgtgccg atgctggggc cattgtggcg
    18481 caactgcgcg ttgccattgc gccagacgat atcgctatta cgctgcatca taaattgtgc
    18541 catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg taatattctg
    18601 gaaattgccc agcgcgaaaa cgaagccacc tgttttggtc acagaacgcc agatgacagt
    18661 ttccttgaat ggcataaacc ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat
    18721 ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt
    18781 gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc gccactgctg
    18841 attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aggcgggcga cggcattact
    18901 atgcagggct cgcaattagc gcagatgctg ggcctggtgc aaggttcacg cttgaatagc
    18961 cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt gaatggcttt
    19021 attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt ttacggtctg
    19081 gatattggca gcgatgcgat aagccgtttt ctgaatcatc cgcattttca ctttgtcgaa
    19141 ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc
    19201 ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct gcgcgtattt
    19261 gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgttaagta ccgtaagcga
    19321 atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata cttcgatgag
    19381 gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta ttcggtgtca
    19441 aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt acagttcacc
    19501 ctcttccgcc cgtttaactg gatgggacca cgactggata accttaatgc agcgcgaatt
    19561 ggcagctccc gcgctattac gcaactcatt ctcaatctgg tagaaggttc accaattaag
    19621 ctaattgatg gcggaaaaca aaaacgctgc tttactgata tccgggacgg tatcgaggcg
    19681 ttgtaccgca ttatcgaaaa tgcgggaaat cgctgcgacg gcgaaattat caacattggc
    19741 aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa
    19801 aaacatccgc tgcgccatca cttcccaccg tttgcgggct ttcgtgttgt cgaaagtagc
    19861 agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
    19921 cgccgctgcc tggactggga accgaaaatt gatatgcagg aaaccatcga cgaaacgctg
    19981 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtcggcttac
    20041 gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccacgtctg ctggaaatct
    20101 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
    20161 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg
    20221 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
    20281 gtcatgccaa tgccgatatc attcgtgaag cggctaaaca tcacgaagtc ggcctgcacg
    20341 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
    20401 acgatattgc acgcggtctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
    20461 ctgccgctgc gggctggcgt gccgaccaga aggtgatcga agcaaaagaa gcgttccatt
    20521 tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccactgctc gaatcaggaa
    20581 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
    20641 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
    20701 cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat aattttgtgg
    20761 atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc gaactgttat
    20821 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
    20881 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct
    20941 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tctggcaacc
    21001 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
    21061 gccccatctg ttagggctac gttatttcga aaaacccatt gccggatact ggattaacag
    21121 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
    21181 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
    21241 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
    21301 ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg gcgggaatgt gcagcttctg
    21361 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
    21421 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
    21481 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
    21541 ggcggttatc agttgcgtac tgacggttct cccctgggga ctggcgatag cgcagcggga
    21601 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactggatga
    21661 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagcctgcc
    21721 gtggctggga ttactccccg gtgcactgta cacaggctgg aaaaaccgca agcattccgc
    21781 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg tcgctaaagg
    21841 taaattgccc acctatattc tttcctgctt tgcatctctg gcaatgctga tggcgcatta
    21901 cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
    21961 cgcttttggc ttcactggca ttattgccac atttgtggtc tccccgtggg gaccaatgaa
    22021 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
    22081 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggtcttttgc
    22141 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagtcat
    22201 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
    22261 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga
    22321 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
    22381 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
    22441 ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
    22501 tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
    22561 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
    22621 aaacaggcaa cctgctttgt ggcgataaac aaacggcgca aacatatcgt gctgtggctg
    22681 ggactggcgc tggcttgtct tggtcttgcc atggtgctct ggctgctggt cttgcagaac
    22741 gtaccggtag gcattgccta cccgatgtta agtctgaatt ttgtctgggt gacgctggct
    22801 gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt
    22861 attggcggca ttgtgatcct cgggagtacg gtgtaatggg cctgatgtgg ggattattca
    22921 gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcgagt catctgccgc
    22981 cgatgacgca cttatgggat tttatcgcgg ctctgttagc ttttggcctg gatgcccgga
    23041 tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat aaaacgctac
    23101 ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg ctggtatgga
    23161 ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca ctactgggag
    23221 tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa caacgttact
    23281 gagttttccc tgccacttta cactgccgtt cccactcatc ggggctataa ctgcttgccg
    23341 agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca ttctgcaaag
    23401 gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg atcattttta
    23461 tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta ttgcagcacg
    23521 attttttgac cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
    23581 acagattaac agataaacct attatcaacg gcaatggaat ggcaaaattg cgaagtgaaa
    23641 ctaatgattt cgtaatcctg gcgttgcacc cactctttta acgcctgacg tgaaatttta
    23701 ataccgccgt ttttcagctc cggcggtaaa gttagccagc gcaccggttg ttgaaaacga
    23761 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
    23821 gctaccggtc gatgaccaaa ctccttgtcg gcaacgggga cgataaacac ctgcaaaacc
    23881 gcaggatgtg cagcaattac gcgctcgact tcttccggct gaataccctc tccgccactg
    23941 aagaacagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
    24001 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
    24061 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
    24121 cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg cttctttcgc acacaccgtg
    24181 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
    24241 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
    24301 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
    24361 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
    24421 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
    24481 tcgtgatcgc caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga
    24541 taagtatgga cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac
    24601 agacgcgtcg gctgccacgt agcggcatgt gcgccttcaa cccgctgaat gtgcagcgac
    24661 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    24721 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    24781 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
    24841 atcacgccgc tgccctcaac cacaccctgc gccgcaaagc ccgtagctaa ctcatcgacg
    24901 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    24961 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcca
    25021 gtacatcagc gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct
    25081 gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc
    25141 gcgccagttg cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc
    25201 ccagcgcatg cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg
    25261 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    25321 ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    25381 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
    25441 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    25501 gatccggaat tgcctccagc aacagattaa ccaccatgcc gtcgcgcacg gcttcgtaca
    25561 atcccacttt gaccttcgcc actttctcgc ctggcatatc agcaagtttg aggatcagat
    25621 cgtcaggatc gccattacac agtggtgccg cacggtaatt ggccgcctgc gggagcgtat
    25681 ctgccagctc cgccaatgca cagcttacgc caaaggcaac ggaaggcatc tgcggtagct
    25741 cgccatcgcc tgccagccag ttatttaccc aagcaagcag cacaatttgc gcctcttccc
    25801 aggtttcctg actgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgcctt
    25861 cacgcaggca aacatacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccatcc
    25921 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    25981 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    26041 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagac ccgcctgacc
    26101 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    26161 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    26221 gacaaggccc atatccagcg cctgttttgc atcgtactga cggcacagga accagatttc
    26281 acgcgctttt ttctgtccga cgatgcgagc catgtaggaa gcgccccagc cgccgtcgaa
    26341 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgccgcga tagtcaggtc
    26401 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    26461 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    26521 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    26581 cgctttatcg cctgcgccag tcagaatgat cacgccgacg ttgtcgtcat aacgcgcatc
    26641 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    26701 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcaaa
    26761 accttcggag cagtcgtgcc attcaaccgg tgcgtaaagc attgcttcat caggataaat
    26821 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat aacgccagcg
    26881 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    26941 tccgccgcca aggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    27001 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    27061 atggcggcaa gggttgcgcc attattgtta ctgcgcagcg ccaccagctc ccggcgttga
    27121 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    27181 ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcgcgttgt
    27241 tcagcatttt gcaacccagg atgcccgcct tcgacaacaa ccccacaaag ccccggcatc
    27301 ccctggcaag ccgccatcat cgccacccgt cccccaagcg agtaccccac caaccagaag
    27361 ttaaggatgt tgtaactaac cagggtttta cgcagtaagt cggtgacatc atcaaatcca
    27421 tcgacgctaa tcgccgccga accaccgtga cctgggagat aaacatacaa ccgtgagtag
    27481 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    27541 aacaccagcc agggtaaacc tggtttgccg tgttttgcct gcgcgtgcag gatcataaat
    27601 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    27661 tttcaattac cgtggtggtt ggcgtgcgcc aggcatcggc aagtgccgtt tcaagttcct
    27721 gccagttttg cggacgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    27781 cgttttgcgg catcagatag aaacgctcgc gctcgctttt cggcgttggc agcagcgaga
    27841 aaatttgccc gccgttgttg ttcaccacaa ttaatatcag cggcgcggaa acctgacgca
    27901 ataacgccag cgcgttgaga tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg
    27961 gtttgccgct tgcccgctga acgccggcgg cagtcgagag cagcccgtcg ataccgctgg
    28021 ccccacggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    28081 gtaccaccag gctgttaccg acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    28141 gatgcgccag ttgcgcttcg ccaaacgcat cgcgacgggc aataaccgcc tgcattgcct
    28201 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    28261 ccagccagtc ggcaatattg gcaattaagc ggcgtccacg atggtgcgcc ggatcaagcc
    28321 gcccttcgat gtcatccaca atccagtact cttcgggttc acagcttgcc tgccattgca
    28381 ggagccgttt gcccgtcagg ctgcttccca gttgcaccac aatttgcgcc tgttgcagtt
    28441 cgctggtcgc tttggcattg cctaaccaga gatcggcaca cggcagcggc tgtccggttt
    28501 gcgacagcac gtcgccaatc agcggccagc caagagtttg cgcccacagt gcaacttttt
    28561 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgtttttgt cgccagaaga
    28621 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    28681 cctgccacca gtcgcccagc cgctgttgcc agctaagccc ggtgtcgtcc atttcgccat
    28741 acagcggttc ggcaaacggg cagttgatat ggactccacc agaatgaagc gtaccgagag
    28801 catggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    28861 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg acgaatcgcc tgattcgcgc
    28921 cgcagtcaat cagctccggc gggcgatcgg cggttaacag gatcagtttt tctccggtta
    28981 acccagcttc aatcagtgcc ggatagagat ttgctaccgc cgtaccggag gtcacaatca
    29041 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct
    29101 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    29161 tagaacgcga gccaggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    29221 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    29281 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    29341 gcgactcatt actattccat ctgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    29401 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc
    29461 acgacattgc cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgctgtaat
    29521 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    29581 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    29641 aaacagatca cattatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    29701 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    29761 aaccgttggc agatatcttc caccaccagc atattctcgc gctggttttt atcatccgcc
    29821 atcagccact cacctaactg ctgcgcctgc ttatcatcag ggtgatttgc taccgttccc
    29881 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    29941 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    30001 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    30061 gcccgagcga gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    30121 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    30181 aacccaggca agggcttgat actcaccagt gtggcgataa attctcttgc cttaatcgca
    30241 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    30301 cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg acgggtcaaa cgcattcagc
    30361 ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    30421 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    30481 tgccagtaga attgcgggta tgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    30541 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    30601 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    30661 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    30721 aggttttaac ggcgtgccag caacagtcct agtaccagcc caacggccgc gcccacacca
    30781 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaactgc ctgcttcgcc
    30841 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    30901 tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    30961 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    31021 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagggtt
    31081 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    31141 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    31201 gccccgaggt aaacaggctt atcaggccag tgatgcgtgc aggtgtcgag ggttttactc
    31261 atcagttgct gccccacttt ttcgccacgc aacgcttcac tgacaatcac ccgaccaata
    31321 acgaccggct caagatcatc atcacttttc agaatcctcg catatgccac cagttcatca
    31381 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    31441 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    31501 tgggaaaccg ataattcaga gtgatgcaga tcttaccatt caatcataat gctctccttt
    31561 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    31621 atttttttag gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    31681 cttaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc
    31741 cagcatcagc tactgcatac cgcctttaac cctgggaaac tggacaagat ttttatcagt
    31801 caccttcatg gcgatcatct ctttggttta cccggcttgc tgtgcagtcg ttctatgtca
    31861 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc
    31921 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    31981 ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt atccgatgga acacccactg
    32041 gaatgttatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    32101 ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca
    32161 atcacgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    32221 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    32281 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    32341 aatagtcgcg gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc
    32401 ggcaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg
    32461 ttacgtgaat gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt
    32521 aacgtttaac tc
//
LOCUS       DAJTXQ010000038        30132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000038 DAJTXQ010000000
VERSION     DAJTXQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.038"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..210
                     /gene="tuf"
                     /locus_tag="OO515_001676"
     CDS             <1..210
                     /gene="tuf"
                     /locus_tag="OO515_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6575628.1"
                     /translation="GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAM
                     DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            440..823
                     /gene="secE"
                     /locus_tag="OO515_001677"
     CDS             440..823
                     /gene="secE"
                     /locus_tag="OO515_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6575629.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            825..1370
                     /gene="nusG"
                     /locus_tag="OO515_001678"
     CDS             825..1370
                     /gene="nusG"
                     /locus_tag="OO515_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6575630.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1529..1957
                     /gene="rplK"
                     /locus_tag="OO515_001679"
     CDS             1529..1957
                     /gene="rplK"
                     /locus_tag="OO515_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6575631.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1961..2665
                     /gene="rplA"
                     /locus_tag="OO515_001680"
     CDS             1961..2665
                     /gene="rplA"
                     /locus_tag="OO515_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6575632.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3078..3575
                     /gene="rplJ"
                     /locus_tag="OO515_001681"
     CDS             3078..3575
                     /gene="rplJ"
                     /locus_tag="OO515_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6575633.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3642..4007
                     /gene="rplL"
                     /locus_tag="OO515_001682"
     CDS             3642..4007
                     /gene="rplL"
                     /locus_tag="OO515_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6575634.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4327..8355
                     /gene="rpoB"
                     /locus_tag="OO515_001683"
     CDS             4327..8355
                     /gene="rpoB"
                     /locus_tag="OO515_001683"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6575635.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8432..12655
                     /gene="rpoC"
                     /locus_tag="OO515_001684"
     CDS             8432..12655
                     /gene="rpoC"
                     /locus_tag="OO515_001684"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6575636.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="OO515_001685"
     CDS             complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="OO515_001685"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6575637.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="OO515_001686"
     CDS             complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="OO515_001686"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6575638.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="OO515_001687"
     CDS             complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="OO515_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6575639.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="OO515_001688"
     CDS             complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="OO515_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6575640.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="OO515_001689"
     CDS             complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="OO515_001689"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6575641.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="OO515_001690"
     CDS             complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="OO515_001690"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6575642.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18342..18442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18495..18971)
                     /gene="rsd"
                     /locus_tag="OO515_001691"
     CDS             complement(18495..18971)
                     /gene="rsd"
                     /locus_tag="OO515_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6575643.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19066..19839
                     /gene="nudC"
                     /locus_tag="OO515_001692"
     CDS             19066..19839
                     /gene="nudC"
                     /locus_tag="OO515_001692"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6575644.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMTEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19879..20943
                     /gene="hemE"
                     /locus_tag="OO515_001693"
     CDS             19879..20943
                     /gene="hemE"
                     /locus_tag="OO515_001693"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6575645.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20953..21624
                     /gene="nfi"
                     /locus_tag="OO515_001694"
     CDS             20953..21624
                     /gene="nfi"
                     /locus_tag="OO515_001694"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6575646.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21667..22257
                     /locus_tag="OO515_001695"
     CDS             21667..22257
                     /locus_tag="OO515_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6575647.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22444..22716
                     /gene="hupA"
                     /locus_tag="OO515_001696"
     CDS             22444..22716
                     /gene="hupA"
                     /locus_tag="OO515_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6575648.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22729..23424
                     /locus_tag="OO515_001697"
     CDS             22729..23424
                     /locus_tag="OO515_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6575649.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="OO515_001698"
     CDS             complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="OO515_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6575650.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24089..25465
                     /gene="zraS"
                     /locus_tag="OO515_001699"
     CDS             24089..25465
                     /gene="zraS"
                     /locus_tag="OO515_001699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6575651.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25462..26787
                     /gene="zraR"
                     /locus_tag="OO515_001700"
     CDS             25462..26787
                     /gene="zraR"
                     /locus_tag="OO515_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6575652.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26784..28073)
                     /gene="purD"
                     /locus_tag="OO515_001701"
     CDS             complement(26784..28073)
                     /gene="purD"
                     /locus_tag="OO515_001701"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6575653.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28085..29674)
                     /gene="purH"
                     /locus_tag="OO515_001702"
     CDS             complement(28085..29674)
                     /gene="purH"
                     /locus_tag="OO515_001702"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6575654.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7054 a         7857 c         8303 g         6918 t
ORIGIN      
        1 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
       61 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      121 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      181 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      241 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      301 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      361 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa
      421 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      481 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtggcgattg tcggcaacta
      541 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      601 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
      661 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
      721 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
      781 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
      841 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
      901 tacgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
      961 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1021 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1081 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1141 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1201 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1261 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1321 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1381 aaaaaagcgg cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt
     1441 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1501 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1561 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1621 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     1681 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     1741 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     1801 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     1861 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     1921 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     1981 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2041 cgcactgctg aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2101 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2161 cggtactggc cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc
     2221 taaagctgca ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2281 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2341 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2401 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2461 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2521 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2581 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     2641 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     2701 tacccccaca tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     2761 cggcaactga gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     2821 gccaacgccg ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     2881 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     2941 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3001 ttaagacgct ctctccgctt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3061 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3121 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3181 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3241 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3301 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3361 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3421 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3481 caattgcacg cctgatggca accatgaaag aagcttcggc tggtaaactg gttcgtactc
     3541 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3601 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     3661 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     3721 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     3781 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     3841 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     3901 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     3961 tgaaaaaatc cctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4021 gttgcagcct gagaaatcag gctgatggct ggtgactttt tggtcaccag cctttttgcg
     4081 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4141 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4201 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4261 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4321 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4381 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4441 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4501 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4561 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4621 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     4681 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     4741 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     4801 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     4861 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     4921 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     4981 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5041 atggagctgg tgccggaacg cctgcgtggt gaaactgcat ctttcgacat cgaagctaac
     5101 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5161 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5221 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5281 ctggatctgt tggctaagct gagccagtct ggtcacaagc gtattgaaac gctgttcacc
     5341 aacgacctgg atcacggccc gtatatctct gaaaccttac gtgtcgaccc aactaacgac
     5401 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5461 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacctgtct
     5521 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5581 atcctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     5641 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc tgttggcgaa
     5701 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     5761 ctgtctctgg gcgatctgga taccctgatg cctcaggaca tgatcaacgc caagccgatt
     5821 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     5881 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc gggtggtctg
     5941 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcatta cggtcgcgta
     6001 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactcttt gtccgtgtac
     6061 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaggtgac cgacggtgtt
     6121 gtaactgacg aaattcacta cctttctgct atcgaagaag gcaactacgt tatcgcccag
     6181 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc
     6241 aaaggcgaat ccagcttgtt cagccgtgac caggttgact acatggacgt atccacccag
     6301 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6361 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg
     6421 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6481 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6541 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6601 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     6661 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     6721 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     6781 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     6841 gtgtcccgtg acaccaagct ggggccggaa gagatcaccg ctgacatccc gaacgtgggt
     6901 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     6961 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7021 gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg
     7081 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7141 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7201 gacctgtctg aagaactgca gatcctcgaa gcgggtttgt tcagccgtat ccgtgctgtg
     7261 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7321 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7381 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7441 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tgtatctggc ggttaaacgc
     7501 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7561 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7621 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     7681 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     7741 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     7801 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     7861 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     7921 ctgaaacttg gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt
     7981 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8041 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8101 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8161 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8221 aacggtcgta ccaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8281 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8341 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8401 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8461 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8521 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8581 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     8641 cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt
     8701 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     8761 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat
     8821 gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg
     8881 tatgaccaac ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga
     8941 agagttcggt gacgaattcg acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa
     9001 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9061 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9121 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9181 tccactggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9241 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9301 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9361 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9421 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9481 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9541 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9601 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
     9661 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
     9721 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
     9781 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
     9841 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
     9901 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg ttctgggtct
     9961 gtactatatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10021 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10081 gcgtatcacc gagtatgaaa aagatgcaaa cggtgaatta gtagcgaaaa ccagcctgaa
    10141 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10201 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10261 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10321 ctatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10381 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10441 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10501 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtga ttaaccgtga
    10561 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10621 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    10681 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    10741 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    10801 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    10861 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga
    10921 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    10981 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11041 cctgctggaa gagaactctg tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac
    11101 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11161 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11221 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11281 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11341 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11401 taccaaagaa agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11461 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11521 agtaagcggt tttgtacgct ttactgacat gatcgacggc cagaccatta ctcgtcagac
    11581 cgacgaactg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    11641 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    11701 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    11761 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg
    11821 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc
    11881 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    11941 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12001 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12061 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac
    12121 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga
    12181 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12241 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12301 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12361 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12421 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12481 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12541 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12601 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    12661 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggtgt tattcagagg
    12721 gcaaattctt cacggaaaat attaagaaag gcagggcgaa acgaggaaga agcttttcgc
    12781 cctggacaac attcctgctg acggcactac cataaaaaaa cacaacgctt tcaacaacct
    12841 tgcgtttttc atcttccgta gcggctcgga ataaatttac gttcttacat tccgtctcat
    12901 agtctttgcg aggcgcgtcc cagatagctg tcccagtctt tccataccgg ctgcaaaccc
    12961 tgagcggtta acgcggcagc aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt
    13021 tccaactcgg ggtgattatc ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg
    13081 ttattgatcg ccagcggaat aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt
    13141 gacagttcaa tctccggtgc aagcagtcgg aaggcgcaga tggtttgcac taactggcgt
    13201 tcatccataa tcgacgcagg ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag
    13261 acagagtaac ggctttgcca gtaatgctgt tgcagccata gcaaatgttc ggcaaccata
    13321 tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg cgccgaggcc tatcttatca
    13381 atccccgcac gccccagccg atccggcgtt tccagccgcc agaagaagtc ctgttttttg
    13441 cctttcaaat gatggcgggt ataagtcgcc tcgtgatatg tctcctgata aaccatcacc
    13501 ccatccagac caagttgctt taactcggcg tattccgtct ccgccagcgg ttgcacttcc
    13561 atttgtagtg aagagaactg ttcacgcagg gcggggagat gacgacgaaa gtaatccatc
    13621 cccactttcg cctgatgttc accagtgact aatagcagat gttcaaagcc catctcccgt
    13681 atagcggcgc tttccctggc aatatccgct tcatccagcg ttttgcgctt gatgcgatta
    13741 ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa gattggaaag ataaagcggg
    13801 acgtagaaac taactgtgtt gccaaatcgc tgacgggtca gacgctgcgc ccgttgggcc
    13861 agtggttcca gatagccact ggcggctggc gataacagcg ccatcatatc gtcgcgggtg
    13921 agttgcgagg catttagcgc ccgttctacg tcagcagccg ttttgccgtt gatacgcagg
    13981 cggatgtcgt cccagtccag ttgtcgccag cgatcgctga aggttttcat gccgatgcct
    14041 ccagaaatcc agtcagcggg ctggtggcat gagcaaaatg actgcggctg cccggtccgg
    14101 actgacgtgc cagcaggcct gcttctaccg ccagacgaaa tgccttcgcc atgttgacgg
    14161 gatcgtccgc tacggcaatc gccgtattca ctaacaccgc gtcggccccc atttccaggg
    14221 cctgcgcggc atggctggga acgccgatgc cagcatcgac aaccaccgga actgtggcct
    14281 gctggataat aatctccagc atggcacggg tttccagtcc ctgattcgag ccaatcggcg
    14341 cgccgagcgg catcaccgct gcacagccga cttcttccag acgtttacac aataccggat
    14401 cggccccgca gtaaggcagc acgacaaatc cctgttgtac cagcatttcg gcggctttca
    14461 gagtttcgat gggatcgggc aacagccagc gggcgtcagg gtgaatctct aattttaacc
    14521 agtttgtgcc taacgcttca cgtgccagat gggcggcgaa aatggcttct tccgctgttt
    14581 tcgccccgga agtatttggc agcagggtca cacccgccgc gataagcggt tcgaggatag
    14641 cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg
    14701 aagcgcggat cgcctccacc atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca
    14761 gatgtgaatc aaacgttttg tccgcaatac gtaacatctc aaccccctgc aataacctga
    14821 aaaagcagga tctggtcgcc atcctgcacg atatgttgcg tccactgctc acgcgggacg
    14881 atttgctgat taatcgccag agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt
    14941 tcgtgaacag tttgcccggc ggcgcactgc atcggttgat cgttaaacag gatctgcatt
    15001 gcttcctccg cataccgggc aaccactggc gcggcgcaac gccaggctgc gccactggct
    15061 cgatttaccg tcgaacagtc ggagttctcc cgcaggtgtc tctataccgc ttaataactt
    15121 aatggcttcc agtgcctgca aagtgcccat aaccccgacc accgggccaa ccacgcccgc
    15181 cgtgcggcag ttgcgttctg gctcctggtt atctggccac aggcagcggt aacacccctg
    15241 ctcccagggc ggcgtcagta ccatcaactg accgccaaat ccgaccgcgc tggcggtgat
    15301 aagcggcgtg ttgagtacca cgcaggcggc attaatctcc tggcgagtcg ccatattgtc
    15361 ggtacagtcg agcaccacat cggcccgtgc aaccgcatct tttaacgcct cacccgttaa
    15421 ccgttgttgt aatgctgtca gttgaatatc gggattcaac tgtgtcagtc gctgatggct
    15481 gacctgcgat ttcgggcgat cgatatcttc agtggtaaag aggatttgtc gttgcagatt
    15541 gcttaaatgc acatcgtcgt catctgccag taccagcgtc ccgacgccag cccccgccag
    15601 gtatagcgca gcaggtgtac ccagcccgcc aagaccaata atcagcacct ggctgtcgag
    15661 cagtttttgc tgcccgtcca gagcgatatc gtcgagcaga atttggcggc tataacgcat
    15721 aaagtcacgg tcattcatcg ccaactcctg caatttccag caactgtgcc gttgccaaac
    15781 gccagtctgc ggcttgagta atggcgctga cgacggcgat actgccgaca cccgttgcta
    15841 tcaccgcagg cgcgcgcgcc agactgatac cgccaatcgc cacggtggga taatccgcca
    15901 gtcgctcaac atgccgtgcc agctgttcca gcccctgcgg tgcagaaggc atctgtttgg
    15961 tttgcgtcgg gaacacatgt cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt
    16021 cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat
    16081 tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac gccatacgcc tgatgcttga
    16141 tcgccagccg ccagtaatcg ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa
    16201 ttgccgccac gacatcggct tccacctctt cattgcgccg atctttgatg cgtagctgga
    16261 gggtacgtac gcctgcatcc aacagacgtt cgatccactg tacgctgtcc accaccgggt
    16321 acagtcctaa acgaaaaggt acaggaggaa aatcaggctg atacatcacg cttcctcctt
    16381 acgcaggtag atttctccgc ctctggcgcg gaagttctcc gacatatccg ccattcccac
    16441 ttcaatggtt tgcgcggcgg cgtaatcacg tacttcctgg ctgattttca tcgagcagaa
    16501 tttcggccca cacatggagc aaaaatgggc gactttacct gactcttgcg gcagggtttc
    16561 atcgtgataa gcgcgggcgg taaacgggtc gagggccaga ttaaactggt cttcccagcg
    16621 aaattcgaag cgggctttcg acatggcgtt atcgcgaatt tgcgcgcctg gatgcccttt
    16681 cgccaggtca gcggcgtgga cggcaatctt ataggtgata agcccctgct taacatcttc
    16741 tttattaggc agacccagat gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa
    16801 ccagccaatc atcgccgcac caatccccga cgtgaagtgg tcatagcccg gcgcaatatc
    16861 ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt
    16921 catattgcgg cggatcatct gcatcggcac gtggcctggg ccttcaatca tcacctgcac
    16981 gtcatattcc caggcaattt tggtcagttc gcccagcgta tgcagctcgg caaactgcgc
    17041 ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac
    17101 gtcataagcg gcacagattt cacaaatttc gcggaagtgt tgatagagga aattttcctg
    17161 atgatgggag aggcaccatt tcgccataat cgaaccgccg cgagagacga taccggtcag
    17221 gcgtttcgcg gtcatcggca catagcgcag cagtacgccc gcatggatag tgaagtaatc
    17281 cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac gcttcccagg taagatcttc
    17341 ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc actgtaccga tcggcaccgg
    17401 gctgttacgc aaaatccact cgcgggtttc gtgaatatag cgaccggtgg agagatccat
    17461 caccgtatcc gctccccagc gcgtggacca taccagcttt tccacttctt cttcgatgga
    17521 agaggtgacc gccgagttgc cgatattggc gttaactttt accaggaaat tgcgaccaat
    17581 aatcatcggc tccgattccg gatgattaat gttggccggg ataatcgcac gtccggcagc
    17641 aacttcatca cggacaaatt ccgcagtgat attttccggc agatgtgcgc caaagctcat
    17701 tcccggatgc tggtggcgta aaacctcgct acggatgcgc tcgcggccca tattctcgcg
    17761 gatggcgatg aattccattt ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt
    17821 gacacggcgt cctgttttgg cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc
    17881 gagaccatca tctgccagcc gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc
    17941 ggtatcgccg cgcgcatcga tccacggctg gcgtagtttt gccagccctt gctgcacgtt
    18001 aatggcgatc tgcggatcac catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt
    18061 ttcttcgtac tgcggctgtt ctttgctacc gccaattagc gtcgggctaa gctggatctc
    18121 acgcatcggc acgcgcacgc cggggtgtgt gccagtgata taaatgcgtt ttgagttggg
    18181 aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg
    18241 ggtcagtttt gttgcagaca tagctcattc caaaaagtca aggacgtggc ttgtcagacg
    18301 acggatgaag caagagacga tcgccgcaaa ggcgatgcga tagcagatta actcttgttc
    18361 ccttcgcagg tattagcctg atcaggttcc gcggatcccg aataaacggt ctcagccagt
    18421 taaggcactc cgacaagaaa ttagccccca caaacggggc gttgaatgta aattacgcgt
    18481 taacagcgca gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc
    18541 agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt
    18601 tgaaactcaa ggtaattatc atgatcgatg gcggtttcca ggctggaatc gtagtaatcc
    18661 ataatctgtt gggtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc
    18721 ccgttgcctt ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag
    18781 taatcgacca ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat
    18841 tctttgccag gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca
    18901 tgtagccagc gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc
    18961 tggttaagca tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa
    19021 aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt
    19081 gaaaaattag atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag
    19141 ggagaattgc catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa
    19201 atcggcgaat ggcaggggga acctgtttgg ttagtacaac agcagcggcg tcacgatatg
    19261 gggtcggtac gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgaggc
    19321 gtacaactgg cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg
    19381 tatccgagca aaaccgaatg ggcgatgctg tgcagccatt gccgtgagcg ttactacccg
    19441 caaatcgccc cctgcattat tgttgccatc cgtcgcgatg attcgatcct cctcgcccag
    19501 catacccgcc atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa
    19561 accctcgagc aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac
    19621 ttgcgttacg tgacttctca gccgtggccg tttcctcagt ctttaatgac cgcgtttatg
    19681 acggaatatg acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg
    19741 tatcgctatg acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata
    19801 gaagatactg tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg
    19861 cactaaggaa cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg
    19921 cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg
    19981 gaatataaag ccacgcgcgc ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag
    20041 ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc
    20101 ttttccgata tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga
    20161 gaaggtccgc gttttacctc gccagtcacc tgcaaagccg acgtcgataa actgccaatt
    20221 ccggacccgg aagatgagct gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa
    20281 ctgaaaggcg aagtgccgct gattggtttt tccggcagcc cgtggacgct ggcgacctac
    20341 atggtggaag gcggcagcag caaagcgttc accgtgatca aaaaaatgat gtatgccgat
    20401 ccgcaggcgc tgcacgctct actcgataaa ctggcgaaaa gcgtcacttt gtatctgaat
    20461 gcgcagatta aagccggtgc tcaggcagtg atgattttcg acacctgggg cggtgtgctt
    20521 accgggcgcg attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta
    20581 ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag
    20641 tggctggaag cgatggcaga aaccggttgc gatgcgttgg gcctcgactg gacaacggat
    20701 atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg
    20761 tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag tagcgactat acttgcaggt
    20821 ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg
    20881 ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca atatcaccgc
    20941 taaggagtga ttatggatct cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct
    21001 gtgatccgcg aggatcgact cgataaagat ccaccggatc tgatcgccgg agtcgatgtc
    21061 gggtttgagc agggcggaga agtgacgcga gcggcgatgg tgctgctgaa atatccctcg
    21121 cttgagctgg tggagtataa agttgcccgc atcgccacca ccatgcctta cattccaggt
    21181 tttctttcct tccgcgaata tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag
    21241 ccggatttag tgtttgtcga tggtcatggg atctcgcatc ctcgccgtct tggcgtcgct
    21301 agccattttg gtttgatggt ggatgtgcca acgatcggcg tggcgaaaaa acggctctgc
    21361 ggtaaattcg aaccgctctc cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc
    21421 gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc
    21481 ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat
    21541 cgtctgccgg agccaacgcg ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg
    21601 cgctatacag caaatcagcc ctaattcagg taaactgcgg ataatttccg tatttgagaa
    21661 ctcaacatgt tacaaaaccc aattcatctg cgtctggagc gcctggaaag ctggcagcat
    21721 gtcactttca tggcttgctt atgcgaacgc atgtacccca attacgccat gttctgccag
    21781 caaaccggtt ttggtgatgg gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg
    21841 ctgaccgtta aagacgcaaa agtaaatttc gacagccaac tggagaaatt tgaagaagcg
    21901 attccttcag ccgacgattt cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg
    21961 gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa
    22021 gtgagtaaga cctccatcac gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa
    22081 atgagcgatg aagagcttaa agaaaaccca gctgtagagc aagaatggga cattcagtgg
    22141 gaaatattcc gacttttagc tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt
    22201 agggcagacc tgcgtgaggc gggtgagagc aatattggta taatttttca gcaataagac
    22261 cagaaaacgt gatttaacgc ctgatttgtc gtacctggag tcttcccttt cgccccccgt
    22321 ctggtctaca tttggggggc gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc
    22381 tattctggca tttccgtcgc actcgatgct tagcaagcga taaacacatt gtaaggataa
    22441 cttatgaaca agactcaact gattgatgta attgcagaga aagcagaact gtccaaaact
    22501 caggctaaag ctgctctgga gtccactctg gctgcaatta ctgagtctct gaaagaaggc
    22561 gatgctgtac aactggttgg tttcggtacc ttcaaagtga accaccgcgc tgagcgtact
    22621 ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg cagctaacgt accggcattt
    22681 gtttctggca aggcactgaa agacgcagtt aagtaagatt gcgtggcagt gaacagtttt
    22741 aacgaagggg tggtttcacc ccttttgtct ttctggcgtc gatcattgat gctggctggc
    22801 gctctgcttc tcactgcctg tagtcataac tcttcacttc ctccctttac cgccagtgga
    22861 tttgctgaag accagggcgc ggtacgcatc tggcgaaaag acagcggcga taatgtgcat
    22921 ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg
    22981 cagggcgata acctcacgct catcaatatc aatgtttaca gcaaaccgcc ggtgaatatt
    23041 cgcgcgcgtt ttgacgatcg cggtgatctg agctttatgc aacgtgaatc cgatggggaa
    23101 aagcagcagc tttctaacga ccaaatcgat ttataccgtt atcgtgctga tcagatccgc
    23161 cagattagcg atgccttacg tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg
    23221 atggaacaga ccgtgaccac ctgcgaaggg caaaccatta aacctgattt agattcgcag
    23281 gcgatagcgc atatcgagcg ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg
    23341 ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa actctgattt ctgtcgctgg
    23401 caacccaacg agaaaacgtt ctgatttacc agtggcccat acccatatga ccgccaccac
    23461 cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg aatacccgct tcagccatcg
    23521 cgatatctcg tttcacccgt aactcatcta acgactgacg caaattctcc atctctttgg
    23581 cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc taacagggca ttgtattcat
    23641 aacgcttcgt caccagttgc tgttgcagtg cgctgctttg agcgtaaaag tcattatgga
    23701 ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac
    23761 cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc cattgctgaa agcgccatca
    23821 ttaccagggc aattttcgtg ttccgtttca tggttaatcc tccagtggtt gtcttacttc
    23881 gggtattgca tcttccgtgc caacgatgaa acgctgatat gacgggtaat ctggcatgat
    23941 aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc atttttactc attgaaactt
    24001 aagcctttgt gttacagcgc agggtaagcg ctgataaaag atggcatgat ttctgctgtc
    24061 agaaagggat gagcaggcaa agaagaagat gcgttttatg caacgttcta aagactcctt
    24121 agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt gggctggtag ggttgtttgc
    24181 ggtgacggtg attcgcgatt atgggcgcga gactgccgcc gccagacaaa cgctgctgga
    24241 aaaaggcagt gtacttattc gcgctcttga atccggctcg cgcgtcggca tggggatgcg
    24301 catgcatcat gcgcagcagc aggccttact ggaagaaatg gccgggcagc ctggagtacg
    24361 ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg catagcaact ccggcatggt
    24421 gggaaaacag ctttattccc cgcaggaaat gcagcagtta catccgggag atgaagaagc
    24481 gtggcggcgg atcgatagcg cagacggcga gcctgttctg gaaatttatc gccagtttca
    24541 accgatgttt gctgctggaa tgcaccggat gcgccatatg cagcagtatg ccgcgacacc
    24601 acaagcaatt ttcatcgctt tcgacgccag taatattgtg agtgccgaag atcgtgagca
    24661 gagaaacacc ctgattatcc tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc
    24721 attcttctgg tatcgccgct atttgcgctc gcgccagctt ctacaagatg aaatgaagcg
    24781 caaagagaag ctggtggcgc tggggcatct tgcggcaggc gttgcccacg aaatccgtaa
    24841 cccactttcc tcgattaaag gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg
    24901 agaagcgcat caactggcgc aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt
    24961 aagcgagctg ctggaactgg ttaagccaac gcatctggct ttgcaggcgg tggatctcaa
    25021 cacgctgatt aaccactcat tacagctggt aagccaggat gcgaacagcc gggagatcca
    25081 gttacgcttt accgccaacg acacattacc ggaaattcag gccgatccgg acaggctgac
    25141 tcaggtcctg ttgaatctct atctcaatgc tattcaggcg attggtcagc atggcgtgat
    25201 tagcgtgacg gccagcgaaa gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa
    25261 gggaattgcg gcagatcagc ttgatgccat cttcactccg tacttcacca ctaaagccga
    25321 aggcaccgga ctggggctgg cggtcgtgca taatattgtt gaacaacacg gtggtacaat
    25381 tcaggtcgca agccaggagg gaaaaggcgc aacgttcacc ctctggcttc cggtcactat
    25441 tacgcgtaag ggcccacaag gatgacgcac gataatatcg atattctggt ggtggatgat
    25501 gacattagcc actgcactat tttgcaggct ttactgcgcg gctggggcta taacgtcgcg
    25561 ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg aacaggtttt tgatcttgtg
    25621 ctttgcgatg tgcgaatggc ggagatggac ggcattgcca cgctaaaaga gatcaaagcg
    25681 ttaaatccgg cgattccggt gctgattatg accgcatact ccagcgtcga gacggcggta
    25741 gaagcgttga aaaccggggc gctggattat ctcatcaagc cgctggattt cgataacctg
    25801 caggcgacgc tggaaaaggc gctcgcgcat acgcacagta ttgacgctga aacgcctgcg
    25861 gtgtctgcca gccagttcgg tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt
    25921 gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga tccacggcga ttccggcacc
    25981 ggtaaagagc tggtcgccag ggcgattcac gccagtagcg cacgtagcga aaaaccgctg
    26041 gtaacgctca actgtgcggc actcaacgaa tccttgctgg aatctgaatt gttcggtcac
    26101 gaaaaagggg cgttcactgg agccgataga cgccgggaag ggcgctttgt tgaggcggac
    26161 ggcggcacgc tgtttctcga tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg
    26221 ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta gcaaccagac tatctcggtt
    26281 gatgtccggt tgattgcggc gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt
    26341 tttcgccagg atctctacta tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg
    26401 caacggcggg aagatatccc tctgctggct gtccattttc ttcagcgctt tgccgagcgt
    26461 aatcgcaagg cggtaaaagg ctttacgcca caggcgatgg atttgctgat ccactacgac
    26521 tggccgggaa atattcgtga actggaaaac gcggtggaac gggcggtggt gctgctgacc
    26581 ggggaatata tttccgaacg cgagctgccg ctggcgattg ccggtacgcc gatcccactg
    26641 gggcaaagtc aggatattca gccgctggtg gaagtcgaaa aagaagtgat tctggcggca
    26701 ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa
    26761 acgctattgg caaaactgtc gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata
    26821 tctttccggc agaagcagtc gtcccagtga atatcggtca ttaaggcata agcgcgtttc
    26881 tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc
    26941 actacctgct cgttatccgc cagtttcgtg cccgcgtgga acactttgcc gcctgccact
    27001 tcttccagcg gcaggccgtg gatcacatcg ccggtgcggt aatcgcccgg atatccaccc
    27061 gcagccatca ccacgccgag agaagcacgc tcatcccact ctgacgtttt ctcgtccagt
    27121 ttgccttcac aggccgccag gcagagctca accagatcgg acttcatgcg cagcataatc
    27181 ggctgggttt ctggatcgcc aaagcggcag ttaaattcga taaccttcgg attgccctgt
    27241 ttgtcgatca tcaggcccgc gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg
    27301 cctttcacgg ttggccagat gatacgttcc atggtgcgct gatgaacttc atcggttact
    27361 accggcgcgg gggagtaagc gcccatcccg cccgtgtttg gtccggtatc tttatcgcct
    27421 acgcgtttgt gatcctggct ggtggccatc ggcagcacat gctcgccgtc taccatcacg
    27481 ataaagctcg cttcttcgcc gtcgaggaac tcttcgataa cgatgcgatg acccgcgtcg
    27541 ccaaaagcgt tgcccgccag catatcgtga acagccgctt cggcttcttc cagcgtcatc
    27601 gccacgataa cgcctttccc ggctgccaga ccgtccgctt taatgacgat cggcgcacct
    27661 ttctcacgca gatacgccag cgcaggttct acctcggtga agttctggta ttccgctgta
    27721 gggatcttat ggcgggccag gaaatctttg gtaaacgctt tcgaaccttc cagttgcgcg
    27781 gcacctgcgg ttgggccgaa gattttcatc ccggcggcgc ggaaggtatc gaccacgcct
    27841 ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa
    27901 tccaacagcg ccgggatatc ggtcacgccg atagcaacgt tttgcagcgc gggttccagc
    27961 gcagtgcctg cattacccgg agcaacaaaa acagtttcaa ccagcggcga ctgcgccgct
    28021 ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca ctaatacttt catctattgc
    28081 tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg
    28141 cggcggcaat cacttcgtca tcacggatag aaccgccagg ctggattacg caggtcacgc
    28201 ctgcagcggc ggcggcatca ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg
    28261 aggaaccttt cacttccaag ccttcatcgg cagctttaat accggcgatt ttcgcggagt
    28321 acacgcggct catctggccc gcgccaatcc cgatggtcat attgtttttg gcatagacga
    28381 tagcgttgga tttcacgaac ttcgccactt tccagcagaa cagcgcatca cgcagttcct
    28441 gttcggtcgg ctgacgttgg gtgacgacgc gcagttcttc cgcaccgacc atgcccaggt
    28501 cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct
    28561 cgccccactg accgcaggtc agaacgcgta cgttctgctt ggcggcggtg attttcaggg
    28621 cttcttcgct ggcggaaggc gcaataatca cttcgacaaa ctgacgagaa atgatggcct
    28681 gtgcggtttc cgcatccagc tcgcggttaa aggcaatgat gccgccgaat gcggaggttg
    28741 ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga attgccgata gccacgccgc
    28801 aagggttggc gtgcttcaca atcacacatg ccggctcggc gaactctttc acgcactcca
    28861 gcgccgcatc ggtatcggcg atgttgttat aagagagggc tttaccctga acctgggttg
    28921 cggtagcaac ggaggcttct ttcacattct cttctatata gaaggcagcc tgctggtggc
    28981 tgttctcgcc gtaacgcata tcctgcttct taatgaagtt caggttcagc gtgcgtggga
    29041 agcgaccggc ggcttctttg ctttcaccgt ggtaagccgg aaccatgctg ccgaagtagt
    29101 tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac
    29161 gggttgcaag cgtcagcgat ccttcgttgt catccatctc tttaataatg gcgtcgtagt
    29221 cgctgctctt caccacgatt gcgacatctt tatggttctt agcggcggag cgcaccatgg
    29281 tcgggccgcc gatatcgatg ttctcaaccg catcttccag cgagcagcct tcacgagcaa
    29341 cagtctgggc gaacggatac aggttaacaa ccaccatatc gataggctgg atctgatgtt
    29401 cttccataat ggcatcgtcc tggccgcgac ggcccagaat gccaccatgt actttcggat
    29461 gcagggtctt cacgcgtcca tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg
    29521 ttaccggcag acctttttct gctaacagac gggcagtgcc ccctgtagac agcagctcca
    29581 caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc ggctttgtca gaaacactga
    29641 gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa tcccctggat ttgactatta
    29701 cagagagcgt tagctgaatt tttcgcgaaa aactcagcta acgcccctaa cggggcatcc
    29761 ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt
    29821 caatggtgat cacaattttg acagtggtta ccgtgggaaa aatacagaaa ttacattgat
    29881 gattgtggat aactctgtgc gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc
    29941 agtcagtcat ttttctgcaa tttttctatt gcggcctgcg gagaaatccc tataatgcgc
    30001 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
    30061 aaatcctgaa attcagggtt gactctgaaa gaggaaagcg taatatacgc cacctcgcga
    30121 cagtgcgcta aa
//
LOCUS       DAJTXQ010000039        29737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000039 DAJTXQ010000000
VERSION     DAJTXQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.039"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..315
                     /locus_tag="OO515_001703"
     CDS             <1..315
                     /locus_tag="OO515_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575655.1"
                     /translation="VPIALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNV
                     ALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIATGKATSGNVNAVTNFHIN
                     YY"
     gene            398..1099
                     /locus_tag="OO515_001704"
     CDS             398..1099
                     /locus_tag="OO515_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6575656.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1124..3723
                     /locus_tag="OO515_001705"
                     /pseudo
     CDS             1124..3723
                     /locus_tag="OO515_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3714..4784
                     /locus_tag="OO515_001706"
     CDS             3714..4784
                     /locus_tag="OO515_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575657.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4796..5338
                     /locus_tag="OO515_001707"
     CDS             4796..5338
                     /locus_tag="OO515_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575658.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5346..5861
                     /locus_tag="OO515_001708"
     CDS             5346..5861
                     /locus_tag="OO515_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6575659.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5827..6564
                     /locus_tag="OO515_001709"
     CDS             5827..6564
                     /locus_tag="OO515_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6575660.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6675..7685
                     /gene="pyrD"
                     /locus_tag="OO515_001710"
     CDS             6675..7685
                     /gene="pyrD"
                     /locus_tag="OO515_001710"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6575661.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7859..8401
                     /gene="zapC"
                     /locus_tag="OO515_001711"
     CDS             7859..8401
                     /gene="zapC"
                     /locus_tag="OO515_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6575662.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8398..9507)
                     /gene="ycbX"
                     /locus_tag="OO515_001712"
     CDS             complement(8398..9507)
                     /gene="ycbX"
                     /locus_tag="OO515_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6575663.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9751..11859
                     /gene="rlmKL"
                     /locus_tag="OO515_001713"
     CDS             9751..11859
                     /gene="rlmKL"
                     /locus_tag="OO515_001713"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6575664.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11871..13778
                     /locus_tag="OO515_001714"
     CDS             11871..13778
                     /locus_tag="OO515_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6575665.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13908..15161
                     /gene="pqiA"
                     /locus_tag="OO515_001715"
     CDS             13908..15161
                     /gene="pqiA"
                     /locus_tag="OO515_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6575666.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKATCPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15166..16806
                     /gene="pqiB"
                     /locus_tag="OO515_001716"
     CDS             15166..16806
                     /gene="pqiB"
                     /locus_tag="OO515_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6575667.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16803..17366
                     /gene="pqiC"
                     /locus_tag="OO515_001717"
     CDS             16803..17366
                     /gene="pqiC"
                     /locus_tag="OO515_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6575668.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17622..17789
                     /gene="rmf"
                     /locus_tag="OO515_001718"
     CDS             17622..17789
                     /gene="rmf"
                     /locus_tag="OO515_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6575669.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17859..18377)
                     /gene="fabA"
                     /locus_tag="OO515_001719"
     CDS             complement(17859..18377)
                     /gene="fabA"
                     /locus_tag="OO515_001719"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6575670.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18446..20206)
                     /locus_tag="OO515_001720"
     CDS             complement(18446..20206)
                     /locus_tag="OO515_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6575671.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20392..20844
                     /gene="matP"
                     /locus_tag="OO515_001721"
     CDS             20392..20844
                     /gene="matP"
                     /locus_tag="OO515_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6575672.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20920..21960)
                     /gene="ompA"
                     /locus_tag="OO515_001722"
     CDS             complement(20920..21960)
                     /gene="ompA"
                     /locus_tag="OO515_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6575673.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22315..22824)
                     /gene="sulA"
                     /locus_tag="OO515_001723"
     CDS             complement(22315..22824)
                     /gene="sulA"
                     /locus_tag="OO515_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6575674.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23043..23672
                     /gene="sxy"
                     /locus_tag="OO515_001724"
     CDS             23043..23672
                     /gene="sxy"
                     /locus_tag="OO515_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6575675.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23635..25797)
                     /gene="yccS"
                     /locus_tag="OO515_001725"
     CDS             complement(23635..25797)
                     /gene="yccS"
                     /locus_tag="OO515_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6575676.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25807..26253)
                     /locus_tag="OO515_001726"
     CDS             complement(25807..26253)
                     /locus_tag="OO515_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6575677.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26376..28430
                     /gene="helD"
                     /locus_tag="OO515_001727"
     CDS             26376..28430
                     /gene="helD"
                     /locus_tag="OO515_001727"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6575678.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28462..28920)
                     /gene="mgsA"
                     /locus_tag="OO515_001728"
     CDS             complement(28462..28920)
                     /gene="mgsA"
                     /locus_tag="OO515_001728"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6575679.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29016..29678)
                     /locus_tag="OO515_001729"
     CDS             complement(29016..29678)
                     /locus_tag="OO515_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6575680.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7651 a         7037 c         7815 g         7234 t
ORIGIN      
        1 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
       61 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
      121 gcgacgaatg ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt
      181 accgaaacgt cgaatattca gttagcggat tctgctgagc aaacagtgac ctttaaagtt
      241 gattatatcg ctacggggaa agcgacttct ggtaatgtga atgcggtaac aaatttccat
      301 attaactatt attaatagaa ctcattaatt gttttattaa ttagtacccc tctagtgttc
      361 tggaggggat attcatattt tttaagagtg actatttatg aaaacttgca taacaaaggg
      421 aattgtgacc gtaagtttaa cggcaatatt actcagctgc tcatccgcat gggcggcagg
      481 taaaggcggg attggacttg cagcaacacg tcttgtttat tcagaaggtg aagagcaaat
      541 ttcactgggt gtacgtaaca ccagtccgga tgttccttat cttattcagt catgggtgat
      601 gaccccagat aataaaaaat cagcagattt tattattaca ccaccgctat ttgtgctgaa
      661 tccggctaat gagaatctgt tacgcattat gtacattggt gcgccgttgg cgaaagacag
      721 agaaaccctt ttcttcacta gcgtacgggc agtcccttca acaacgaagc gggaagaggg
      781 aaataccctg aagattgcca cacaaagcgt catcaaactt ttctggcgac caaaaggttt
      841 agcgtatccc ttaggcgagg ctccggcgaa actgcgttgc acttcgtcag ctgacatggt
      901 tacggtcagt aacccaacac cttatttcat taccctgaca gacctgaaaa tagggggaaa
      961 ggtagttaaa aatcaaatga tttccccctt tgataaatac caattctctc tgccaaaggg
     1021 ggccaaaaat agcagcgtaa cgtatcgaac catcaatgat tacggggcgg aaacgccaca
     1081 actcaactgt aaatcgtaag ccgtcttcag ttaagagagc gagatgtata gaactcatcg
     1141 acaacacagc ctgttaagct ctggtggagt gccatcgttt attggtgggc tggtggtgtt
     1201 tgtgtcggca gcgttcaatg cacaagctga aacctggttc gatcctgctt ttttcaaaga
     1261 tgatccctca atggtggccg atttgtctcg tttcgaaaaa ggacaaaaaa taacgccagg
     1321 ggtttatcgt gtcgatattg ttctgaatca gacaattgta gatacgcgca acgtcaattt
     1381 tgttgagata acgccagaga aggggattgc cgcctgtttg acgactgaaa gcctggatgc
     1441 aatgggtgtt aatactgatg cgtttccggc ttttaaacaa ctggacaaac aagcgtgtgt
     1501 gccattggcg gagattattc cggatgccag cgtaacgttt aatgtgaata aactccgtct
     1561 ggaaatttca gtaccgcaaa tcgccatcaa aagtaatgcc cgtggttatg tcccccctga
     1621 acgttgggat gaagggatca acgcgctatt actgggatat tcgtttagcg gggctaacag
     1681 tattcatagc agcgcagaca gtgattctgg cgacagctat tttctgaatt taaacagtgg
     1741 cgttaattta ggcccatgga gattgcgcaa caattcaaca tggagtcgca gtagtggcca
     1801 aaccgcagaa tggaagaatc tcagcagcta tttgcagcgg gcggttattc cactaaaagg
     1861 cgaactgacc gtaggtgatg attatactgc aggcgatttt ttcgatagtg tcagctttcg
     1921 tggtgtgcag ctggcgtcag atgacaacat gctgccagac agcctgaaag ggtttgcgcc
     1981 tgtggtgcgt ggtatcgcca aaagcaatgc ccagataacg attaagcaaa atggttacac
     2041 catttaccaa acttatgtat cgcctggtgc ttttgaaatt agtgatctct attccacgtc
     2101 gtcgagcggt gatttgttag ttgaaatcaa agaagcggac ggcagcgtca atagctacag
     2161 cgtaccgttt tccagcgtgc cattactcca gcgtcagggg cgaatcaaat acgcggtgac
     2221 actggcgaaa tacagaacca atagtaatga acagcaggag agcaaatttg cctaggccac
     2281 gttgcagtgg ggcggaccgt ggggaacgac atggtatggt ggtggacaat atgctgaata
     2341 ttaccgtgcc gccatgtttg gtctgggatt taaccttggc gatttcggag caatttcgtt
     2401 cgatgcgacc caggcgaaga gtacgctggc agaccaaagc gaacataaag gtcagtcata
     2461 tcgttttctg tatgccaaaa cgctcaacca attgggcact aattttcaat tgatgggcta
     2521 tcgctattcg acgtcgggtt tctacaccct ttccgacacc atgtataaac atatggatgg
     2581 ctacgaattt aatgacggtg atgatgaaga tacgccgatg tggtcgcgtt attacaattt
     2641 gttttacacc aaacgtggca aactgcaggt caatatctcc cagcaattag gcgagtacgg
     2701 ttcgttttat ttaagtggta gccagcaaac ttactggcat accgatcaac aggatcggct
     2761 attacagttt ggctacaaca cgcaaattaa agatctctcg ctggggggtt cctggaacta
     2821 cagtaagtcc cgtggtcaac ctgatgctga tcaggtgttt gcactaaatt tttccctgcc
     2881 gctcaatctg ttgctcccca gaagtaatga tagctatacc aggaaaaaaa ttacgcctgg
     2941 atgacctcta acaccagcat cgataacgaa gggcacatta cacaaaacct gggtttaacg
     3001 gagacactac tcgatgacgg taacctgagc tacagcgtgc aacagggata taacagcgag
     3061 gggaaaacgg ctaatggtag cgccagcatg gactacaaag gggcgtttgc agatgcccga
     3121 gtgggctaca actacagcga taacggcagt caacaacaac tgaactacgc tctttcaggc
     3181 agtttagttg cccattcaca gggcattacc ctggggcaat cgctggggga aactaacgtg
     3241 ctgattgcag caccaggcgc agagaatact cgtgtggcga acagcaccgg gctgaaaact
     3301 gactggcgcg gatataccgt tgtcccctat gccacttctt atcgggaaaa tcgaatcgca
     3361 cttgatgcgg catcgttaaa acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt
     3421 cccaccaaag gggcgttggt tctggcggag ttcaatgccc atgcgggagc aagggtatta
     3481 atgaaaacat caaagcaggg tataccgctg cgttttggcg cgatagcgac gctggacggc
     3541 gtacagacta atagcggcat tattgatgat gatggctcgc tctatatgtc cggtttgccc
     3601 gctcagggag cgataactgt acgctggggc gaagctcccg atcaaatttg tcatatcagt
     3661 taccagctta ccgaacaaca aattaactct gcgattacgc gaatggatgc catatgcaga
     3721 taatctttgg agaaaaatgc gtgttattac tacgactatt ttttgccgcc gtcttaatgc
     3781 tatggtgcgc tcaaactgct gcttatagcg ggcagtgtca taccactcag gggaatccgt
     3841 atattggcgt caattttggc gttaaaaccc tggaggaaga agaaaatacg gctggagtag
     3901 ttaaagacaa attttatcag tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg
     3961 ataaagacaa tgtcagaagt ggccgatggg cattcgccgc ggattcaccg ttagtctatt
     4021 taggcgacaa ctggtacaaa attaatgact atcttgccgc caaagtttta ttgcaggtta
     4081 aaggcagttc tcctactgcg gttcctttcg aaaacgtggg cacaggggca gatacacgat
     4141 ggcatatttg cgatcccggc ggtcaacgtt taggtggcca gggggctagc ggtaatagcg
     4201 gtagcttttc cctgaaaata ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg
     4261 cgctggcgcg attatttgaa tgctacaaca tacccgcagg tgattcctgc acgactacag
     4321 gtacaccggt tttagtgtat tacctgtctg gtacgatcaa ttcacttggc tcatgttccg
     4381 tcaatgccgg agaaacaatc gaggtcgatc tgggcgacgc atttgcggct aactttcgtg
     4441 ttgtagggca taagcctctt ggggccagaa cggcagaact tgcaattcca gtcaggtgta
     4501 acacgggaaa cgcggggtta gttaacgtca acctgagtct gacggcaacc acagacccca
     4561 gctatcccca ggcgattaag acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc
     4621 agaacaacat tatttcccct gctggtggaa cattaccgct ctctattcct gatgatgcag
     4681 acagtatcgc gcgaatgaat gtctatccag tcagcacgac aggtgtacca ccagaaaccg
     4741 ggcgatttga agccacggca acggtgagaa taaattttga ttaacgcgtg aacgtatgaa
     4801 gaaagaaacg atatttcagt gcgttatctt gttctttagc attcttaaca tccatgtcgg
     4861 gatggctggg cctgaacaag ttagtatgca tatttatggg aatgtggtcg atcagggctg
     4921 tgatgtcgcc accaaaagtg cattacaaaa tattcatatt ggtgatttta atatcagtga
     4981 ttttcaggcc gcgaataccg taagcactgc tgctgatttg aatatcgata tcaccggttg
     5041 tgccgctagt attactggcg cggacgtcct ttttagcggc gaggctgaca cccttacgcc
     5101 gacactgctc aaactaactg acacaggcgg aagcggtggt atggcaacgg ggattgccgt
     5161 gcaaattctt gatgcgcaaa gtcagcaaga aatcccgctc aatcaggtcc agcctcttac
     5221 gcccttaaaa gccggggata acacactcaa atatcaactt cgttataagt ccacaaaggc
     5281 gggagcaacg ggcggtaatg cgacggcggt tctctatttt gatctggttt accagtgaag
     5341 gagtgatgtt gaaacggata atctggattc tgttcttact gggattaacg gggggctgtg
     5401 agctatttgc ccatgatggc acggtcaaca ttagcggatc gtttcgccgt aatacatgcg
     5461 tgctggcaca ggatagcaag caaatcaatg tgcagttagg cgatgtctcg ctgacacgtt
     5521 tttttcatgg gaattatggc ccggagaaat ctttcatcat taatttgcag gattgcggaa
     5581 cagatgtcag cacggttgac gtcacctttt caggaacccc cgacggagtg cagagcgaaa
     5641 tgctgtccat tgaaagtggt acggatgcag ccagcgggct ggcaattgcg attctggatg
     5701 atgcgaagat attgattccg cttaaccagg cgagtaaaga ctacagccta catagcggta
     5761 aggtgccatt aactttttat gcgcaattgc gacctgtcaa tagcgatgtg cagtcgggta
     5821 aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa cagattggcg
     5881 ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg aattgcgggc aggagtggtg
     5941 gtcggcggaa cgcgatttat ctttccggca gacagagaat cgatatctat tttactgact
     6001 aataccagtc aggaatcctg gcttattaac agtaaaatca accgcccaac gcgttgggcc
     6061 gggggggaag cgtcgacagt gccagcacca ttactggccg ctccgccact tattctcctg
     6121 aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat cttgcctgtg
     6181 gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc catccggcaa agttgaaaat
     6241 cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc cgaaggtttg
     6301 ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc gcagggtgta
     6361 caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt aaatggtaaa
     6421 gcgttaagta atgcgggagt tgtgccgcct aaaagccagc gtcagacaag ctggtgtcag
     6481 gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg attacggcgg gttgagtgca
     6541 aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac gtttgcgttt tttttgccgc
     6601 aggtcaattc ccttttggtc cgaactcgca cataatacgc ccccggtttg cacaccggga
     6661 atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag ctcgatccag
     6721 agcgcgctca tgagtttact tttcagcaat tacgccgtat tacaggaacg ccgtttgaag
     6781 cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg acgtttaaaa
     6841 atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgac gcgttaggcg
     6901 cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag ccaggtaatg
     6961 acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt atgggcttta
     7021 ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa ggcccattat gacggcgtcc
     7081 tgggtattaa catcggcaaa aataaagata cgccagtgga gcagggcaaa gatgactatc
     7141 tgatttgtat ggaaaaaatc tatgcctatg cgggatatat cgccatcaat atttcatcgc
     7201 cgaatacccc aggattacgc acgctgcaat atggtgaagc gctggatgat ctcttaaccg
     7261 cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg ccgatcgcag
     7321 tgaagatcgc gccggatctt tctgaagaag aattgatcca ggttgccgat agtttagttc
     7381 gccataatat tgatggcgtt attgcaacca ataccacact cgatcgttct cttgttcagg
     7441 gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg tccgcttcag ttaaaaagca
     7501 ccgaaattat tcgccgcttg tcactggaat taaacggtcg cttaccgatc atcggtgttg
     7561 gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggtgcc tcactggtgc
     7621 aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc gttacccata
     7681 tctaattatt tcttcgaatt cctctttata agataatcag ggctttattt tcagccctgg
     7741 ttgttttata ttcatcgctg ttgcttattt agacattttg tacttttatt gttgaggtta
     7801 ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg agaggcagat
     7861 gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg atcgaatgat
     7921 gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaga tgttgactcc
     7981 agatgccttt tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt tctcttttga
     8041 agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg tgctgaatgc
     8101 actggtagca attcgctatc tcaaaccgca aatgccgaaa agctggcatt ttgtttccca
     8161 tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga gcgatactca
     8221 cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcactat gcctgctggc
     8281 acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca ttaaaatcat
     8341 gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac aggcagtcta
     8401 acgcgccaac ttaagtgcag tcttcggtac acagctacag caaagaatgg tgccatcatc
     8461 gcccattgct gattttttca gcggcatgac ttcgccttct aaaagctgaa cacggcaact
     8521 tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt ctaattgctc
     8581 cagcaacacc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa tatctacatt
     8641 tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc cgtaaatttt
     8701 tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc cgctattacg
     8761 ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc ggaaagattg
     8821 caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc tgacggtggt
     8881 gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa tgcgaatcac
     8941 tttccagcga tcttcttccc aggctgacgc gccggaaacc accagattgg ggcggaattg
     9001 ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg cttcgttagc
     9061 aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc gtttcacgcg
     9121 ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa atccacttag
     9181 ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc aaacttcggt
     9241 tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc catctggtgc
     9301 ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccatct ggggaaactg
     9361 gcgagcggta ataaacgtac cgtcaggttc cgtgatcata aagatgcgat cgaaggccag
     9421 accactgaca tcagccagag catgtgtaag accaatgccg cgcatcgatt taacaggatg
     9481 aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata agccctcaac
     9541 tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc gcctgtccca
     9601 ttaggctatt ttatttgtca gtttggccct gggttgtgct cgaaatcctc acgtacttgt
     9661 gtacgctccg gtttctccgc gctgtccatg tccagactga ctgcaacaat tacgcctact
     9721 gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag tacggcccgt
     9781 gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga atgccaggtg
     9841 gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag cctgatgtgg
     9901 agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta cagcgattta
     9961 gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc tggcgcgacc
    10021 ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta cggcgcgatg
    10081 aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg tccaaatgtt
    10141 gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac cgccagtatc
    10201 gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg tgctggtatt
    10261 gcgccgatca aagaaaccct ggcagccgct attgtgatgc gaaccggctg gcagccagga
    10321 acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc ggcgatgcta
    10381 gcgaccgatc gcgcaccagg cttgcaccgt gggcgttggg gctttagcgg ctgggtgcag
    10441 catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgtgc ccgtaaaggc
    10501 ctggctgagt atagctctca tttttacggt tcggacagcg acgcacgggt gattcaacgt
    10561 gcgcgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga ggtgaaagat
    10621 gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gcacagtgtt gagcaacccg
    10681 ccatacgggg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag cctgctgggt
    10741 cggatcatga aaaaccagtt tggtggctgg aatctctctt tgtttagtgc ctcgccggat
    10801 ctgctaagct gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa cggcccgctg
    10861 gactgcgtac agaaaaatta ccacgttgcc gaaagcactc cagacagcaa accggcgatg
    10921 gtagcggaag actacgccaa ccgcctgcgt aagaacctca aaaaattcga gaagtgggct
    10981 cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctaccgga atataacgtt
    11041 gccgttgacc gttatgccga ctgggtggtg gtgcaggagt atgcgccgcc aaaaactatt
    11101 gatgctcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat ttcggtactg
    11161 gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg caaaaaccag
    11221 taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa cgctcacttg
    11281 tgggtgaacc tgacggatta tctcgatact ggtctgttcc tcgatcaccg tatcgcccgc
    11341 cgtatgctcg gtcagatgag caaaggcaaa gatttcctca acctgttctc ttataccggt
    11401 agcgccaccg tgcatgcggg attaggcggc gcgcgcagca ccaccaccgt ggatatgtcg
    11461 cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcctgac cgggcgtgcg
    11521 catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga acagtttgat
    11581 ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga tgcgtttgat
    11641 gttcagcgcg atcatatggc gctgatgaaa gatttgaaac gtctgctgcg tgcaggtggg
    11701 acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg cctggcaaaa
    11761 ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt cgcccgtaac
    11821 cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta atgtcattaa
    11881 tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac
    11941 tgcatatcga agataacgaa cgtgtttgtc tggtgggtcg caacggcgcg ggcaaatcga
    12001 cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt atttacgagc
    12061 aagatttgat tgtagctcgt ctgcaacagg agccgccgcg taacgttgag ggtagcgttt
    12121 atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc tatcacgata
    12181 tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg gcgaaggttc
    12241 aggaacagtt ggatcaccac aacctgtggc agctggaaaa ccgcatcaac gaagtgctgg
    12301 cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct ttccggcggc tggttgcgta
    12361 aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt gatgaaccga
    12421 caaaccacct ggatattgaa accatcgact ggctggaagg gttccttaaa accttcaatg
    12481 ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg acgcgcattg
    12541 ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag tacctgcttg
    12601 aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat cgcaaactgg
    12661 cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt aatgaaggcc
    12721 gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga acgtcgcgaa gtgatgggta
    12781 ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc gaaatggaag
    12841 acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc caggttctac
    12901 gtggcgacaa aattgccctg attggtccga atgggtgcgg caaaaccacg ctgctgaaac
    12961 tgatgctcga tcagcttcaa gcggacagcg ggcgtattca cgttggcacc aaactggaag
    13021 tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg atggataacc
    13081 ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta ttgggctatt
    13141 tgcaggactt tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg ctttctggcg
    13201 gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac ttattgattc
    13261 ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa gaactgatcg
    13321 acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc gataacaccg
    13381 ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc ggcggttatc
    13441 atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg gtgaaaaaaa
    13501 ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct
    13561 ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg gaggcgaagc
    13621 tggaagccct acagacgcag gtggcggatg cttccttctt cagtcagcca catgagcaga
    13681 cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa gcctttgaac
    13741 gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac
    13801 agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc atgaaaacca
    13861 acactttgtt agccatctgt aaaatagcgc atcattaagg agtaccaatg tgcgaacatc
    13921 atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc
    13981 ttgagcatgg tcagaaagcg acatgtcccc ggtgtggcac aacgttaacc gtggcgtggg
    14041 atgcccctcg gcagcgtccg accgcctatg cgttggctgc actgttcatg ctgttgctgt
    14101 ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa attacattac
    14161 tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc tttttcctgt
    14221 tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg gtgaatcgcg
    14281 cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa ctcaaaacct
    14341 ggggaatggc ggagattttc ctcgctggtg tgctggtcag tttcgttaaa ctgatggctt
    14401 acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt gtcctgcaac
    14461 tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc ccgatgccag
    14521 aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg cgttcgtgct
    14581 cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt agtaccaaag
    14641 ggtacgttcg gcgtagaaac agccagcagt ggacactcgc gctgcttgta acgtccatca
    14701 tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta ggctcgaaga
    14761 tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct tatcccgtcg
    14821 ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc gccatcgcgt
    14881 ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg catttgattt
    14941 atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt atcgcggtgc
    15001 tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg ggtgcattaa
    15061 tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac ccgcgtttgt
    15121 cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga atctaataat
    15181 ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt tcctatcgtc
    15241 acggcgctca ttggggcctg ggttcttttt tatcattaca gccatcaggg actggaagtg
    15301 accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat taaaagccgt
    15361 agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac gcacgttgaa
    15421 atcaaagtgc ggctgaattc tggtatggaa aaattgctgc ataaagacac cgtcttttgg
    15481 gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc tgggaacgct gctgtctgga
    15541 gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg
    15601 gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct cgatagcaaa
    15661 aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg ggtaggttcg
    15721 gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca gttatcaact gttcatcaat
    15781 gcaccttatg accgactggt gaccagcaat gttcgcttct ggaaagatag tggcattgcg
    15841 gttgatctga cgtcagcggg gatgcgtgtg gagatgggct cattgacaac gctgcttagt
    15901 ggcggtgtta gctttgatgt gccggaaggt ctggatttag ggcagccagt ggcaccgaaa
    15961 acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta caccgatcac
    16021 attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg agctccggta
    16081 gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc gccgaatatg
    16141 cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga gccagagcgg
    16201 ctgaaaatgc agcttggcga aaatgcggat gttgttgagc accttggcga attgttgaaa
    16261 cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact gtatgttgat
    16321 cttgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa tggttatcag
    16381 attatcccga ctgttagcgg cggcctggcg caaatccagc aacgactgat ggaagcgttg
    16441 gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag tacgctttct
    16501 gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa caagatcctc
    16561 gccagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg tgaattgaat
    16621 cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt ggcggatatg
    16681 cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct caatgagaag
    16741 agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa
    16801 caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc agctccggcg
    16861 aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa agtaccgcca
    16921 gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat ctggcgggga
    16981 atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac aacttgtggg
    17041 ccagcccgtt ggatcaacaa ttgcgcaaca ccctggttgc caacctgagt acgcaactgc
    17101 ccggctgggt ggttgcctcc cagcctctgg gaagcgccca ggacacgctc aatgttaccg
    17161 taacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag tggctgttga
    17221 accaccaggg acaactgatc aaacgtccgt tccgtctgga aggagtgcaa actcaggatg
    17281 gttacgatga gatggttaaa gtactggcca gtgtctggag tcaggaagcc gcttctattg
    17341 cgcaagagat aaagcgtcta ccttaattat aaagatttgt aaatataacc gtctccggta
    17401 tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa aagttgttcc tctttacatt
    17461 ttttgtacaa ccgacatacc cgtgtagctc acaaatatga cagtggcgtg aattttgcgc
    17521 attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt agtaatcact
    17581 gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga caaaaacgag
    17641 atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga cgctcaaaag
    17701 aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc tggcgagaag
    17761 ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa cctccgcatt
    17821 gcggaggttt cgccttttga tactctgtct gattataatc agaaggcaga agtatcctgg
    17881 aacagaccga ctttcaggtc gctggcggta tagatcaaac gaccatcaac cagcacttct
    17941 ccatccgcca ggcccataat cagacgacgg ttaacaatgc gtttaaagtg aatacggtag
    18001 gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc aacgcccagt
    18061 gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctaccag ctgccacatt
    18121 gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa taaagtggca tccgaagaac
    18181 cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa gttaccaccc
    18241 gtttcggtca ttttgaccac acggtccatc atcagcatat tcggtgctgg caattgcggg
    18301 cctttagcgc caaacagttc accgcgacca gaggcaagaa ggtcttcttt tgtataggat
    18361 tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc aggatagcta
    18421 acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc caacgtaatg
    18481 gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt tcctggatgg
    18541 tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat aacggcagtg
    18601 cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc gctttcacta
    18661 gttcactgtg aagacttaaa tggcgaacgt tagctgtggg gataatgaca ccttgtttcc
    18721 cggttaactc acgttgctgg caaatagcaa agaagccttc gattttctca tttaaaccac
    18781 cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata ctctgattca
    18841 ccggcacatc ggcgagggcg cttatcaggg cgcagagttc agccatcgag gcactatctc
    18901 catcaacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg atctgttgct
    18961 caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc gcatggatat
    19021 tgccgccaag ctccgctttg cgttcgatgt cggtgaattc accatcgcca atatgcacaa
    19081 cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg
    19141 aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa
    19201 ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc tgctgcagca
    19261 ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg acctctttac
    19321 actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg gtgtagcgtg
    19381 ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg tgcaggtaag tgattatgtc
    19441 tggcggtaaa tgtcacccag cgacaccact gggttactga ctccgcatcg acaatctgca
    19501 gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc atctcctgga
    19561 aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat ggcatcgaag
    19621 gcacagagac ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt
    19681 taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta cgcaaagaga
    19741 tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta atgtcgccat
    19801 taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca gcgacaactt
    19861 gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg atggagtggc
    19921 cggaaacctc atagtgaccg cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg
    19981 cattggcaat aagattcaga tactcagact cttccggggc cttcgccagc ataaaagagg
    20041 aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagccgcggt tgagtatcac
    20101 tgaataaagg atcgttttcg tcaatcaaat gtggctgagc aaatatttcc tgatagctat
    20161 cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt
    20221 agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg tagaaagcac
    20281 cgacaatact ccaggcatgg gcgttaaagc tcacaggatg gagattcttt tcttcactgg
    20341 cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc tatgaaatat
    20401 caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa gaagcatcgc
    20461 gaaggggagt taatcacccg ttacatagaa gccagtgccg cccaggaagc cgtcgatgtg
    20521 ttgctctcgc tggaaaatga acccgtgctg gtaaatggct ggattgacaa gcatatgaat
    20581 ccggaactgg tcaatcgcat gaagcaaacc attcgggcaa gacgaaagcg ccattttaat
    20641 gcggaacacc agcatacgcg caaaaaatcg atcgatctgg aatttattgt ctggcaacgt
    20701 ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt tcagctgatt
    20761 gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa gcaggatctg
    20821 caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta aaggcaaaaa
    20881 aaaccccgca gcagcggggt ttttctacca gacgagaact taagcctgcg gctgagttac
    20941 aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca ggcagtcgat
    21001 cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc
    21061 cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt aatcaacaac
    21121 agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga tgcggtcggt
    21181 gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct ggctgtacag
    21241 ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt tgaacagaac
    21301 gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg gagccggagc
    21361 aactactgga gctgcttcgc cctgaccgaa acggtaggaa acccccaggc tcagcatgcc
    21421 gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc
    21481 cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccgccagcga agaccggaga
    21541 aacgccggtg tcgtggtttt taccataaac gttggattta gtgtctgcac gccataccat
    21601 accacccaga cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca gtttagcggt
    21661 cagttgaacg ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt acggcatacg
    21721 acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt aaccaccaaa
    21781 agcaccagcg cccagttggt tttcatgggt cgggccattg ttgttgatga aaccagtgtc
    21841 atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc
    21901 ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat
    21961 tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt
    22021 taaatccttc accgggggat ctgctcaatt aactctaccg atatcttcgg cttatgccga
    22081 gcacccctgg cgatgtaaag tctacaacgt agttgaaaac ttacaagtgt gaactccgtc
    22141 aggcatatga aaaaaaagtc ttgtataagg tatgtttaat cttttttgtc agcgacaatt
    22201 tacagaagaa aatcgcggaa accgcttcag acaagcctcc gcaaggaaaa ttagtcacga
    22261 ctgaaagcat tggctgggcg acaaaaaaag ttccaggatt aatcctaaat ttacttaatg
    22321 atacaaatta gagtgaattt ttagcccgga aagttgtctc gtggcgtgag aggatgcgct
    22381 taccggacgc ataataaacc ccatagcgtt accttcattt gccgcatcaa caagttcagc
    22441 atgctcttct tcagtcaaat catctgccaa ccaaccgatc accacactgt aattgcccgt
    22501 gcgtaaagcg cgaaccattg actccacagt gtggcaaggg gagagctggc taatctgcat
    22561 tactttcgtt aagggtagcc cagatgcctg aacccattcc cgactcagtt tttgttgcgg
    22621 tgttaaccag agttgccagc gcgattgctg accgagttgc tgtaacaatg gcaacagtag
    22681 aagttgcgtc atcatgggct gatcttcgcg atagacaact tcactgataa gcccggctgt
    22741 agtgttttcc gtagagacac gcgcaatttt acttgctgcg gatgagaacg acgaagaacg
    22801 atgtgcatag cctgaagtgt acataatcaa tccagcccct atgagttact gtatggatgt
    22861 acagtacatc cagtgacaac aaagatcaac cctattttcg gaaagagcct cgcaaatttt
    22921 gtcgttggtg acgggaaaac ataaattaat cttgcccctt aagaataagt tgcctatttt
    22981 cgtagttaac ggatccgtta atgtgaatca ttcttttatg ttatgatttt aaaaggaatt
    23041 ttatgaaaag cctctcctat aagcggatct ataaatcaca agaatacctg gcaacgttgg
    23101 gcacaattga ataccgatca ttgtttggca gttacagcct gaccgttgac gacacggtgt
    23161 ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg tgagcaaagt gcacagtact
    23221 gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg tggccgatcc gttaccctca
    23281 attactatcg ggttgatgaa agtctatggc gaaatcaact gaagctggtg cgtctgtcga
    23341 aatattctct cgatgcagcg ctgaaagaga aaagcacgcg caatacccgg gaaagactga
    23401 aagatttgcc caatatgtct tttcatctgg aagcgattct cggggaggtg gggattaagg
    23461 atgtacgggc gttacgtata cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa
    23521 acagtctggt gacagaaaag attctgttta tgcttgaagg tgccattatc ggcattcatg
    23581 aagctgcgct cccggtggca cgccgccagg agcttgcaga atgggctgac tctcttacgc
    23641 cgaaacagga gtttcctgcg gaacttgagt aatctggcgt tgcagacgac caatctcagg
    23701 cagtaatgca atcaataatc caacttgttg tacgaccaga ggttctttgc tgtctgcccg
    23761 tggttcaagt tgctgcatcc gctgtttcag gctagctaat gcctcattga cgcgttcttc
    23821 atcagcaggt tgatgatgta acgcgtcatc aacatagcaa actgcgtcat caagaaacgc
    23881 cagaatttca ggattagtta actgctcccg gtgagcaccg agggctgaga tatagctggt
    23941 aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg cgaatttgcg gggtaacgtt
    24001 cggctcgctg gacatatttg ataccaccga cgccagctca gcatcacggt tgtgtgcatc
    24061 gcggcgggca atacgatacg ccagacggtt atcacgcccc tgatggtatt gctccagtat
    24121 ggcatcgaga taccgacagt tggcctctgt ggcgcgttcg agcatgcgcg gcagattgcg
    24181 aaactgccag tcaggccaga tgtagctcac tgccgcccac gcaatggcac aaccaatcag
    24241 cgtatcgatt acgcgaggta acgctacttc aaaaccttca cccagtaagt taaaacacag
    24301 tagcaccaaa agtgtgatga acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa
    24361 aaagagcacg ccggtaataa ccagcagcac cagctgccct tccagtgatg gcacaaacca
    24421 cagcacagga atgccaatgg cgatacctac cagcgtacca ataatcctta acttcaggcg
    24481 gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa cttgtcagca agatccaata
    24541 cccgtgatgc attccggtta tctgaatgat ggcgtagccg aagcacaata ccagcgacat
    24601 tcttaccgca tgacggaaga gggcggattc cggcgtgaag tgacggctaa gacgcagcca
    24661 gatatcactc aacccgtgcg ggctgtcatc agcgagctca ttttcgtcat tattatgggg
    24721 tagtgcctgg gcctgttctg attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt
    24781 gttcagcaaa aatcccagtg ttttgagtaa atcggcgggt gcgccgttat cgcgcatccg
    24841 ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa tgcggatcat gttgataagg
    24901 ctgacgcaac aaaatacagc gtgacagttg ctggcacgcc tggccctgca tcgacatcag
    24961 ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg
    25021 aatatgagaa gagctggcac gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt
    25081 gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt
    25141 ctgattcaat gtcgccatca gcagaccgtt ggcgagagcc aaatcgtaca gcggtgcctg
    25201 gctttcatct tcaatatcag gatcaaacat gcgcgacttg agctcaagat aacgcgccag
    25261 ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg accgggaaca gcagatgacc
    25321 aataagtgtc aggacgttgt accagacggc accggccagc agatacatcg gctgctgata
    25381 ccagtgctca tacagtgatg ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa
    25441 ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa atgaagccgc tggtagagag
    25501 cgttaagcca atcgcaaata gccagggcca gggaaacagc aattctactg aggccgaggc
    25561 gataaaaaag cagaacagcg taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc
    25621 gagatcggtc agcgccgctg ccaccatccc cagcgttagc ggaatcgtca gttttacatc
    25681 acccagccac cacggaaacg ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata
    25741 cagccaggcg ctgttccagg tatagcgttt gagcaaagga cttagcataa aggccatatt
    25801 agtggattat tcaaaacgac gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac
    25861 cgataccacg cgacgaccaa ccggccatag tgcaatagcg gcaattttaa agttcgcaat
    25921 gccgacagga atgccaatga ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag
    25981 gcataaccac cagccaaaga aaatcagcca gaaaatattc aataccgtac cgccagtatt
    26041 cagcagcaca tttttgccag ccgggttcag ttcatcgaca tgaatagctt cattgccata
    26101 aggcaccaga gatagtttag tgatctccca gcaggatcgt gtcagcggta aggtaaaaat
    26161 cagcacaata ctgaccagag tcgccaacag ccagcccaga gtggtggcaa atccgccaag
    26221 cacaaagttc agaatgttca aaacggtacg cataaaacct cgctttactg tggttttcag
    26281 taatgggcgg caattgtaac gtttttttgg gctggagcac gttttctctg acggttacac
    26341 tgataagaaa taatttcgtg tggatctaca gagtcatgga actgaaagcg acaacgcttg
    26401 gaaaacgtct ggcacagcac ccttacgatc gggcggtgat cctcaatgcc gggattaaag
    26461 tctccggcga tcgccacgaa taccttattc ctttcaatca attactggcg attcactgta
    26521 agcgcggtct ggtatggggc gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc
    26581 tgcacggcac cgaatggggc gagacgcagc gtttttacca tcatcttgat gctcactggc
    26641 ggcggtggag tggcgagatg agcgaaattg cgtctggtgt tttacgccag caactggatt
    26701 tgattgccac gcgcactgga gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc
    26761 agcaacaaat ccgccaggct ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat
    26821 tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg gttgaaagat attgaaagcg
    26881 ctcggttgca gcataaccag gcgtataccg aagccatgct taccgagtat gcggattttt
    26941 tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg
    27001 agcattctct gttagtgctg gcaggtgcag gaagcggaaa aacgtcggtg ctggtggccc
    27061 gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt
    27121 ttggtcgcaa agccgctgaa gagatggacg agcggattcg cgaacggcta cataccgaag
    27181 acattaccgc acgcacgttt catgcgctgg cgctgcatat tattcagcag ggcagcaaaa
    27241 aagttccgat agtcagcaaa ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg
    27301 ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc
    27361 tgacggaaga aatgcagtgg tcagtgccag aaggtaactt ctgggatgat gaaaaattac
    27421 agcgtcgcct tgcctctcgc ctcgatcgct gggtaagtct gatgcggatg cacggtggtg
    27481 cacaggcaga aatgattgcc agtgcacccg aagagattcg cgatctgttc agtaaacgta
    27541 tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg
    27601 tcgatttttc gggccttatt catcaggcga ttgtgattct ggagaaaggt cgctttatca
    27661 gcccgtggaa gcatattctg gttgatgaat ttcaggatat ctcgccgcag cgggcagcgt
    27721 tgttagcggc attacgcaag caaaacagtc agacgacgtt gttcgctgtt ggtgatgact
    27781 ggcaggcgat ttaccgattc agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa
    27841 actttggtga aggcgaacgt tgtgatttag acacgactta ccgttttaac agtcgtatcg
    27901 gtaaggtggc aaaccggttt attcagcaga acccaggcca gctgaaaaag ccgctaaaca
    27961 gcttaaccaa tggagacaaa aaagccgtca cgttattgga tgagagtcaa cttgacgctt
    28021 tgctggataa gctctctggt tatgccaaac cggaagagcg cattctgatc ctggcgcgtt
    28081 accatcacat gaggcctgcc agcctggaaa aagcggcaac acgctggccg aagttgcaaa
    28141 tcgactttat gaccattcat gccagcaaag ggcaacaggc ggattacgtc atcatcgttg
    28201 gcttgcagga gggaagtgat ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg
    28261 cgctactgcc accggttgag gatttcccgg acgctgaaga acggcggtta atgtacgtgg
    28321 cgctgacccg ggcacgccat cgggtatggg cactgtttaa caaagagaat ccctctccct
    28381 ttgtggaaat actgaaaaat ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg
    28441 gcgtttgcca cctgtgcaat attacttcag acggtccgcg agataacgct gataatcggg
    28501 gattagaata tcgaccgcgt cgttgaaatg cggcgactgg attatgaagt ctgccgttgc
    28561 cacgttggtg gcgaccggaa tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc
    28621 aggatcgtgc ggcacggcat ttagtggatc ccagaagaaa atcaatacat caattttccc
    28681 ttctgagatc aatgcgccaa cctgctggtc accccccatt gggccactca acatcgcgtt
    28741 gacgttcatg ccggtcgcgc gggaaattaa gttaccggta gtgcctgttg catacagtac
    28801 gtgttgttcc agtaacggtt gatgccgttc cacccagctc atcagcattt gtttgcagtg
    28861 atcgtgtgcc accagcgcaa tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat
    28921 aatgtacatc cgtagttaac tttcctacag attactgtaa gcacttatcg ctgcaagata
    28981 aagaccgaaa aagcctgcgc acaggcacaa aaatctcagg aagatggttg tttttccgcc
    29041 cactgcagga aagtatttcg cgtttgtggg tcagccagtt taaaccaata cttcagccgt
    29101 tgttctgtga gcacctgaga ctgcggtgga atagcatcca gctcggaaac acccgaaagc
    29161 agcgtactgt catctgccat catcgtggca aattgcggta gtgaagcgcg tttcgcggat
    29221 ttgttatagc gttcaacctc gacctcataa tcaatcgttt ttgccgttga ggtaatggcg
    29281 agaatatcca gctttaccgg aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc
    29341 gcggcatcaa aatggttagc ttcccgctca ttctccaggc gaggcaggcg aaaattgacc
    29401 tggttgatga gctgggtatt aaaactgacc accagcggtg gggagatata cagccgttct
    29461 tcactattgg aaagatgaat cgtcttctca acgcgaaaca ctaactgatg cggtccatta
    29521 tccagttcaa tgctgtccgc accgcgcagc agagagctgg aaaccttctt gccgtcgagt
    29581 accagaagat cgacatcggt tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa
    29641 cataatgcaa ttaaggtggt cacgatgccg gttttcatag gtagctcctg tcaaaagacc
    29701 gcccgaataa taatgtgtca aaatttttct ttaatca
//
LOCUS       DAJTXQ010000040        29538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000040 DAJTXQ010000000
VERSION     DAJTXQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.040"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..679
                     /gene="dgt"
                     /locus_tag="OO515_001730"
     CDS             <1..679
                     /gene="dgt"
                     /locus_tag="OO515_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR6575681.1"
                     /translation="VEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSL
                     SRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLY
                     KNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIE
                     SRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYR
                     RLMAVEQ"
     gene            809..2233
                     /gene="degP"
                     /locus_tag="OO515_001731"
     CDS             809..2233
                     /gene="degP"
                     /locus_tag="OO515_001731"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6575682.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2388..3545
                     /gene="cdaR"
                     /locus_tag="OO515_001732"
     CDS             2388..3545
                     /gene="cdaR"
                     /locus_tag="OO515_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6575683.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3634..4020)
                     /locus_tag="OO515_001733"
     CDS             complement(3634..4020)
                     /locus_tag="OO515_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6575684.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4182..4994)
                     /gene="yaeI"
                     /locus_tag="OO515_001734"
     CDS             complement(4182..4994)
                     /gene="yaeI"
                     /locus_tag="OO515_001734"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6575685.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5049..5873)
                     /gene="dapD"
                     /locus_tag="OO515_001735"
     CDS             complement(5049..5873)
                     /gene="dapD"
                     /locus_tag="OO515_001735"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6575686.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5904..8576)
                     /gene="glnD"
                     /locus_tag="OO515_001736"
     CDS             complement(5904..8576)
                     /gene="glnD"
                     /locus_tag="OO515_001736"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6575687.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8638..9432)
                     /gene="map"
                     /locus_tag="OO515_001737"
     CDS             complement(8638..9432)
                     /gene="map"
                     /locus_tag="OO515_001737"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6575688.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9800..10525
                     /gene="rpsB"
                     /locus_tag="OO515_001738"
     CDS             9800..10525
                     /gene="rpsB"
                     /locus_tag="OO515_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6575689.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10783..11634
                     /gene="tsf"
                     /locus_tag="OO515_001739"
     CDS             10783..11634
                     /gene="tsf"
                     /locus_tag="OO515_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6575690.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11781..12506
                     /gene="pyrH"
                     /locus_tag="OO515_001740"
     CDS             11781..12506
                     /gene="pyrH"
                     /locus_tag="OO515_001740"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6575691.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12798..13355
                     /gene="frr"
                     /locus_tag="OO515_001741"
     CDS             12798..13355
                     /gene="frr"
                     /locus_tag="OO515_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6575692.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13447..14643
                     /gene="ispC"
                     /locus_tag="OO515_001742"
     CDS             13447..14643
                     /gene="ispC"
                     /locus_tag="OO515_001742"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6575693.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14832..15590
                     /gene="ispU"
                     /locus_tag="OO515_001743"
                     /gene_synonym="uppS"
     CDS             14832..15590
                     /gene="ispU"
                     /locus_tag="OO515_001743"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6575694.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15603..16460
                     /gene="cdsA"
                     /locus_tag="OO515_001744"
     CDS             15603..16460
                     /gene="cdsA"
                     /locus_tag="OO515_001744"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6575695.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16472..17824
                     /gene="rseP"
                     /locus_tag="OO515_001745"
     CDS             16472..17824
                     /gene="rseP"
                     /locus_tag="OO515_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6575696.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17854..20286
                     /gene="bamA"
                     /locus_tag="OO515_001746"
     CDS             17854..20286
                     /gene="bamA"
                     /locus_tag="OO515_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6575697.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20408..20893
                     /gene="skp"
                     /locus_tag="OO515_001747"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20408..20893
                     /gene="skp"
                     /locus_tag="OO515_001747"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6575698.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20897..21922
                     /gene="lpxD"
                     /locus_tag="OO515_001748"
     CDS             20897..21922
                     /gene="lpxD"
                     /locus_tag="OO515_001748"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6575699.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22027..22482
                     /gene="fabZ"
                     /locus_tag="OO515_001749"
     CDS             22027..22482
                     /gene="fabZ"
                     /locus_tag="OO515_001749"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6575700.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22486..23274
                     /gene="lpxA"
                     /locus_tag="OO515_001750"
     CDS             22486..23274
                     /gene="lpxA"
                     /locus_tag="OO515_001750"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6575701.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23274..24422
                     /gene="lpxB"
                     /locus_tag="OO515_001751"
     CDS             23274..24422
                     /gene="lpxB"
                     /locus_tag="OO515_001751"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6575702.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24419..25015
                     /gene="rnhB"
                     /locus_tag="OO515_001752"
     CDS             24419..25015
                     /gene="rnhB"
                     /locus_tag="OO515_001752"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6575703.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25052..28534
                     /gene="dnaE"
                     /locus_tag="OO515_001753"
     CDS             25052..28534
                     /gene="dnaE"
                     /locus_tag="OO515_001753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6575704.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28547..29506
                     /gene="accA"
                     /locus_tag="OO515_001754"
     CDS             28547..29506
                     /gene="accA"
                     /locus_tag="OO515_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6575705.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7156 a         7119 c         8157 g         7106 t
ORIGIN      
        1 ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg aggcgtgggg
       61 tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg aaaaatcacg
      121 ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac
      181 cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc ctgcgatttt
      241 cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa
      301 gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg agcagcttga
      361 attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct
      421 gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga
      481 gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag
      541 taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct
      601 actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct
      661 gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg
      721 cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg
      781 cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact
      841 ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc
      901 agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc
      961 agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa
     1021 tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac cgtttcagag
     1081 ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac agaaattcat
     1141 ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca
     1201 tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta agttcgacgc
     1261 gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc agaacccgaa
     1321 aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt
     1381 agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg tctctgcgct
     1441 ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg atgcagcgat
     1501 caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa
     1561 caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta tcccgagtaa
     1621 catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct
     1681 ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag ttgacgccca
     1741 gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa
     1801 agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg ccgcactgcg
     1861 tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac tgcgcgacgg
     1921 taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg ttgattccag
     1981 ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt
     2041 ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga
     2101 tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagtgct
     2161 cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca ccatctacct
     2221 gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc tccttacaat
     2281 ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt gcacaatgcc
     2341 gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg gctggctggc
     2401 atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc atcgatacca
     2461 atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt gagcgtattg
     2521 gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc gatatcgatg
     2581 acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg ttacggctgg
     2641 aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg cgtaaatatg
     2701 gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg atgcacctgt
     2761 tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag gctgaggaga
     2821 atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat caaccgcgag
     2881 tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca atggcggagt
     2941 tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg gcaattgttt
     3001 cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag
     3061 aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac ggccagctgc
     3121 gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc cgatcctatc
     3181 gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt cgctgctatt
     3241 tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac tggcaggcca
     3301 acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc ttgctgcgac
     3361 gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca aaggcgctgt
     3421 ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg accgggcttg
     3481 atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg gatgaagagc
     3541 ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc cgaagggtag
     3601 aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt tttccagatc
     3661 ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta agtcgtcgag
     3721 gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat caagctcatc
     3781 aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct gacccacacc
     3841 atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct tggcgaaaaa
     3901 ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag
     3961 gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat
     4021 agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc atggcccgcc
     4081 cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat
     4141 caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag ttccagcatc
     4201 gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc acgggttgtg
     4261 tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc ttcgacaggg
     4321 gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg gccgcacagc
     4381 atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg tgccagcatc
     4441 agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc ccataagtca
     4501 ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc ctggttaaac
     4561 aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt ttctgtacca
     4621 acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc aaggggggag
     4681 aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta atcgccgccc
     4741 agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga aatcaggctt
     4801 aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa tggtgctgcg
     4861 ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag ctcccagtaa
     4921 tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg caacaatcgg
     4981 cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg ctgcccgtgt
     5041 gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc
     5101 gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc ttttgacggc
     5161 agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat ttcgccggtt
     5221 tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat gacggaacct
     5281 tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca gttatcttca
     5341 atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac gccaccggaa
     5401 aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt atcaaccatg
     5461 gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg
     5521 gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc ttctttctgg
     5581 aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta gcggctttct
     5641 gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac
     5701 cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc accggaatcc
     5761 agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt ggctggcgtg
     5821 atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa
     5881 ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac
     5941 cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc ggtggcaatt
     6001 atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt
     6061 cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc gagcgcgata
     6121 agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc ggtttcaaca
     6181 gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca gctactttgt
     6241 gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga tagcgggcta
     6301 ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt
     6361 tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga
     6421 taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta
     6481 agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg ccaggccagt
     6541 tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc
     6601 gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg gtgatggcga
     6661 acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggca
     6721 aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg
     6781 caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt ttgaacttct
     6841 tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc tgtcaccgac
     6901 atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga gttcagcccg
     6961 tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc gccgccgcgt
     7021 cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc
     7081 ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc aaaactctcc
     7141 agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac
     7201 tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac gctatggcga
     7261 tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct caaaaacagt
     7321 tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgtaac
     7381 tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac
     7441 atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc
     7501 gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc gggaagggcg
     7561 aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta
     7621 acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc actgtaattc
     7681 agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta acggctgacg
     7741 accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt taattccgcc
     7801 cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt tgcgccaaaa
     7861 tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg gctacttttg
     7921 atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg gttctgttct
     7981 tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt
     8041 tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat taaattggtg
     8101 gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg cacactatga
     8161 ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga
     8221 tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc
     8281 tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc
     8341 gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc ctcaatcaac
     8401 tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata
     8461 tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg attttgcggt
     8521 tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg
     8581 cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatctta
     8641 ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca
     8701 gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt
     8761 ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg
     8821 ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg agtcatagtg
     8881 cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat attcacgaac
     8941 gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt cgcgcaggtt
     9001 aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga tgcggcacag
     9061 acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa
     9121 accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttcgc
     9181 atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg atttcggata
     9241 gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca
     9301 gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag
     9361 cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat
     9421 tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg
     9481 ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc
     9541 actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt
     9601 attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat
     9661 acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca
     9721 tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt
     9781 ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt
     9841 tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc
     9901 gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct
     9961 ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg
    10021 cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca
    10081 tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct
    10141 gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca agaaagaagc
    10201 gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat
    10261 gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa
    10321 agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga
    10381 cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct
    10441 gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga
    10501 agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat agttcgagtt
    10561 gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga
    10621 gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca
    10681 ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt
    10741 gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca
    10801 tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca
    10861 ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct
    10921 attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc
    10981 gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac
    11041 gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact
    11101 gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt
    11161 ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct gggttcttat
    11221 cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt
    11281 aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc agaagacgta
    11341 tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat gcagtctggt
    11401 aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa
    11461 gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg
    11521 aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga aggcatcgag
    11581 aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatcg
    11641 aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct
    11701 tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt
    11761 gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc
    11821 ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac
    11881 tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg
    11941 tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg
    12001 ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg
    12061 cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt
    12121 gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc
    12181 tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta
    12241 tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg
    12301 atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc gaagtgctgg
    12361 aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac
    12421 cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa
    12481 aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc
    12541 gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg cagtttggtc
    12601 acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga gcactgctcg
    12661 gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta atatgtttaa
    12721 tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac tgagacaagt
    12781 tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta cgcatggaca
    12841 aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt gcttctccca
    12901 gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg cgtcagctgg
    12961 caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt
    13021 ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg aactctgcgg
    13081 gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca
    13141 aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg
    13201 cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac gacgatcgcc
    13261 gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt gaagcggcgc
    13321 tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa aaacgccgct
    13381 cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct caactctgga
    13441 tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg cagcacgctg
    13501 gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat
    13561 gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt aatggacgat
    13621 gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg caccgaagtc
    13681 ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg
    13741 gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa
    13801 accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt tatggacgcc
    13861 gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc catttttcag
    13921 agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca aaatggcgtg
    13981 gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt gcgcgatttg
    14041 gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg acgtaaaatt
    14101 tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc gcgttggctg
    14161 tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt gattcactca
    14221 atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg
    14281 ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat
    14341 ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta tccatgcctg
    14401 aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt gaatgccgca
    14461 aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga tatcgctgcg
    14521 ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg
    14581 ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc
    14641 tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt cagtgatata
    14701 gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc gtggttttac
    14761 acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg tcagggaata
    14821 aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca gcgcatggct
    14881 gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag caagggagga
    14941 ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg
    15001 ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac tggaaccgac
    15061 cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat agcgaagtaa
    15121 aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt cgctttaact
    15181 cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat accggtctga
    15241 cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga gtcaggcaac
    15301 tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa gagatgctaa
    15361 accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg actggggggg
    15421 agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt tactttacag
    15481 atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat gcctttgcta
    15541 atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga tggggggcgc
    15601 ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt
    15661 tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc atgctggcag
    15721 cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta tggttggcgg
    15781 tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat caccgaaata
    15841 ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg attgtcgcgc
    15901 tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct aaaacattgc
    15961 gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg gcgttacggg
    16021 cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat gtcatgatcc
    16081 tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt aaacataagc
    16141 tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga ctagcgaccg
    16201 cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct cccgtcacct
    16261 tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg accgagagta
    16321 tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga cacggtggta
    16381 ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc ttgttgttac
    16441 tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg atttggcttc
    16501 gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt tctgggttgc
    16561 ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg cgctctggcg
    16621 gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg gcggttatgt
    16681 caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc atgccttcaa
    16741 taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg caaacttcat
    16801 ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg tacgtccggt
    16861 ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac caggtacgga
    16921 actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt
    16981 cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca gcgaccaacg
    17041 gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag aagatccggt
    17101 atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg aaaatgtgca
    17161 gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga
    17221 tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata acccgggtaa
    17281 atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat taatcccgga
    17341 gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga aagtcattcc
    17401 tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc
    17461 cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa aattgatcac
    17521 cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg gggctgggat
    17581 gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta gcgtgaactt
    17641 agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc tgttccttgc
    17701 gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt gttatcgcat
    17761 tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt tctctcggtt
    17821 atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt gctcatagcg
    17881 tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt gaaagatatt
    17941 catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat gccggtgcgc
    18001 acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct gtttgctacc
    18061 ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt tcaggtaaaa
    18121 gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa agatgacatg
    18181 ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc
    18241 attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa atatagcgcc
    18301 agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc
    18361 caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca tgctttcacc
    18421 accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg gaacgtggta
    18481 ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct gcgcagctac
    18541 tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag tctgacgcca
    18601 gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta caagctttct
    18661 ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca gctgactaag
    18721 atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga tgacatcaaa
    18781 aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac
    18841 gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg tttctacgtg
    18901 cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg
    18961 cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa ggagcgtctg
    19021 aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc gggtagcccg
    19081 gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt caacttcggg
    19141 attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca ggataactgg
    19201 ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca gacctatgct
    19261 gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg tggtcgtctc
    19321 ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa caagagttat
    19381 ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg tgcaggtctg
    19441 ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg
    19501 tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa aacggacgac
    19561 ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt cccgacagat
    19621 ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa cgaatactac
    19681 aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa atgggttgtt
    19741 ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat gccgttctac
    19801 gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa tacaattggt
    19861 ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta tgattacgaa
    19921 tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac
    19981 gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag cgataagtat
    20041 gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga tacaaactgg
    20101 gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat ccgtatgtct
    20161 gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc ctacgcccag
    20221 ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat cggtaaaacc
    20281 tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa
    20341 tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg atggtaagga
    20401 gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg caacttctgc
    20461 tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc aggtagcgca
    20521 gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca
    20581 gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga aagcgggcag
    20641 cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg ctcagaaagc
    20701 gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca aactggttac
    20761 tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc tggttgttga
    20821 tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg acgtactgaa
    20881 acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc agttggatgc
    20941 agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc aatctgcaca
    21001 aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag gcttgtgcca
    21061 ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt
    21121 agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata ccacgccgca
    21181 gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa
    21241 cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg ataacgtgat
    21301 tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt cgcgtctctg
    21361 ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga tccagtccgg
    21421 aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact gggtgaagat
    21481 cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct gcacaaccat
    21541 cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg ataaccagtg
    21601 ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg gcgtcattat
    21661 ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg taatcaacgg
    21721 gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga tgcgtcccat
    21781 cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa
    21841 aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat cgcttgagcg
    21901 caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta cggcctgtct
    21961 cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt tgacaggaag
    22021 agtatcttga ctactaacac tcatactctg cagattgaag agattttaga acttctgccg
    22081 caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg
    22141 cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt ccctggaaaa
    22201 ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg tattctggcg
    22261 tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg tattgacgaa
    22321 gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt cactttcgaa
    22381 aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg taaagtagtt
    22441 tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat tgataaatcc
    22501 gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc gaacgcacac
    22561 attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac cgtactgaaa
    22621 tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat ttatcagttc
    22681 gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac ccgtgtggaa
    22741 atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac agtccagggc
    22801 ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca cattgcgcac
    22861 gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc gggtcacgta
    22921 tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt ctgcatcatt
    22981 ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc tccttatgtc
    23041 attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc
    23101 ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat ttatcgtagc
    23161 ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac atatccggaa
    23221 gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg ttaatgactg
    23281 aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat atcctggggg
    23341 ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt ggtgttgccg
    23401 ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa ctggcggtaa
    23461 tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt gccgatctga
    23521 caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg cccgacttca
    23581 atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat tacgtcagtc
    23641 catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc accgatctgg
    23701 tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct
    23761 ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat gccgcccgtg
    23821 atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg agccgtggtg
    23881 cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg cgccagacat
    23941 atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag cagtttgaac
    24001 gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg atgggccgtg
    24061 aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc ctggagtgta
    24121 tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc ttctggttgg
    24181 cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg ggcagagagt
    24241 tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg gcgctgttac
    24301 cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt gaactgcatc
    24361 agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag ttagcacaat
    24421 gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag tcggacgcgg
    24481 gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc gcccgattgc
    24541 cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct atgaagagat
    24601 caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa tcgacgagct
    24661 gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc
    24721 gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc ctgcgatggc
    24781 tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc tggcgaaagt
    24841 gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg gttttgccca
    24901 acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg gcgcgaccga
    24961 acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt cctgattctt
    25021 gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg tacacctgcg
    25081 ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt tggtaaaaaa
    25141 ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc tttgtggtct
    25201 ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg gggcagattt
    25261 taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac tggcggcgaa
    25321 caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc gcgggtacgg
    25381 tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag ggttgatcct
    25441 tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta acagcgcgct
    25501 ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt ttctcgagct
    25561 gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg aactggcgga
    25621 agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca gcagcgactt
    25681 tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg atcctaaacg
    25741 cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt gcgagctgtt
    25801 tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt gtaacgtaac
    25861 cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga gcaccgaaga
    25921 ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt tattccctga
    25981 cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga ctgaacttca
    26041 ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat ttatccagtg
    26101 gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg gttcactggt
    26161 ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc tgttcgaacg
    26221 tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct gtatggagaa
    26281 acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg tatcgcagat
    26341 catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc gcgtgctggg
    26401 gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc cggggatgac
    26461 gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag cggatgaaga
    26521 agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta acgccggtaa
    26581 gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc cgctttactg
    26641 cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg aatacgccgg
    26701 actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact gggcgctgga
    26761 gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg ctgcgatccc
    26821 gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg cggtattcca
    26881 gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact gcttcgaaga
    26941 tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga tggtggataa
    27001 ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac agtggcagca
    27061 tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc aggaacaggt
    27121 catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata tgctgcgtcg
    27181 tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat ttgctgaagg
    27241 tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc tggtggagaa
    27301 attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg tgtcatatca
    27361 aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa tgaccgccga
    27421 tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga tggggctgaa
    27481 aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg acgacggcga
    27541 aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg aggccatcat
    27601 cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg cccgtaccga
    27661 caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg cgtttgaccg
    27721 tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa aagcggcaga
    27781 tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc tggccgaaga
    27841 gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc aggtggtgtt
    27901 agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca accagtattt
    27961 aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc cgacagaacg
    28021 tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg tcaccaagcg
    28081 cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg aagtgatgtt
    28141 gtttactgac gccctggata aataccagca attgctggaa aaagaccgca tacttatcgt
    28201 cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg ctcgcgaagt
    28261 gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct cgctgacgga
    28321 caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag cccaccgctc
    28381 tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc ggttgcgttt
    28441 tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc gtggcctcat
    28501 tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga gtctgaattt
    28561 ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc tgactgcggt
    28621 tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc gtctgcgtga
    28681 aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc agattgcgca
    28741 actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg catttgatga
    28801 atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg tcggtggtat
    28861 cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc gtgaaaccaa
    28921 agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca aagcactgcg
    28981 tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg acaccccggg
    29041 ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg cacgcaacct
    29101 gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg aaggtggttc
    29161 tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat acagcaccta
    29221 ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg acaaagcgcc
    29281 gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga aactgatcga
    29341 ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga tggcggtatc
    29401 gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca ctgaagattt
    29461 aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg caaaagttct
    29521 gaaaaagggt cacttcgg
//
LOCUS       DAJTXQ010000041        28777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000041 DAJTXQ010000000
VERSION     DAJTXQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.041"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(369..575)
                     /locus_tag="OO515_001755"
     CDS             complement(369..575)
                     /locus_tag="OO515_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6575706.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            888..977
                     /gene="kdpF"
                     /locus_tag="OO515_001756"
     CDS             888..977
                     /gene="kdpF"
                     /locus_tag="OO515_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6575707.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            977..2650
                     /gene="kdpA"
                     /locus_tag="OO515_001757"
     CDS             977..2650
                     /gene="kdpA"
                     /locus_tag="OO515_001757"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6575708.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2673..4721
                     /gene="kdpB"
                     /locus_tag="OO515_001758"
     CDS             2673..4721
                     /gene="kdpB"
                     /locus_tag="OO515_001758"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6575709.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4730..5302
                     /gene="kdpC"
                     /locus_tag="OO515_001759"
     CDS             4730..5302
                     /gene="kdpC"
                     /locus_tag="OO515_001759"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6575710.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5295..7979
                     /gene="kdpD"
                     /locus_tag="OO515_001760"
     CDS             5295..7979
                     /gene="kdpD"
                     /locus_tag="OO515_001760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6575711.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7976..8653
                     /gene="kdpE"
                     /locus_tag="OO515_001761"
     CDS             7976..8653
                     /gene="kdpE"
                     /locus_tag="OO515_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6575712.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8928..9032
                     /locus_tag="OO515_001762"
     CDS             8928..9032
                     /locus_tag="OO515_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6575713.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9343..11541
                     /gene="speF"
                     /locus_tag="OO515_001763"
     CDS             9343..11541
                     /gene="speF"
                     /locus_tag="OO515_001763"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6575714.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11538..12857
                     /gene="potE"
                     /locus_tag="OO515_001764"
     CDS             11538..12857
                     /gene="potE"
                     /locus_tag="OO515_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6575715.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12913..14553)
                     /gene="pgm"
                     /locus_tag="OO515_001765"
     CDS             complement(12913..14553)
                     /gene="pgm"
                     /locus_tag="OO515_001765"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6575716.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14579..15124)
                     /gene="seqA"
                     /locus_tag="OO515_001766"
     CDS             complement(14579..15124)
                     /gene="seqA"
                     /locus_tag="OO515_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6575717.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15309..16073
                     /gene="ybfF"
                     /locus_tag="OO515_001767"
     CDS             15309..16073
                     /gene="ybfF"
                     /locus_tag="OO515_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6575718.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16213..16506
                     /gene="ybfE"
                     /locus_tag="OO515_001768"
     CDS             16213..16506
                     /gene="ybfE"
                     /locus_tag="OO515_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6575719.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16646..17176
                     /gene="fldA"
                     /locus_tag="OO515_001769"
     CDS             16646..17176
                     /gene="fldA"
                     /locus_tag="OO515_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6575720.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17386..17472
                     /gene="uof"
                     /locus_tag="OO515_001770"
     CDS             17386..17472
                     /gene="uof"
                     /locus_tag="OO515_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6575721.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17465..17911
                     /gene="fur"
                     /locus_tag="OO515_001771"
     CDS             17465..17911
                     /gene="fur"
                     /locus_tag="OO515_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6575722.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17996..18322)
                     /locus_tag="OO515_001772"
     CDS             complement(17996..18322)
                     /locus_tag="OO515_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575723.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18372..19778)
                     /gene="chiP"
                     /locus_tag="OO515_001773"
                     /pseudo
     CDS             complement(18372..19778)
                     /gene="chiP"
                     /locus_tag="OO515_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20356..22020)
                     /gene="glnS"
                     /locus_tag="OO515_001774"
     CDS             complement(20356..22020)
                     /gene="glnS"
                     /locus_tag="OO515_001774"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6575724.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22279..24225)
                     /gene="nagE"
                     /locus_tag="OO515_001775"
     CDS             complement(22279..24225)
                     /gene="nagE"
                     /locus_tag="OO515_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6575725.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24558..25358
                     /gene="nagB"
                     /locus_tag="OO515_001776"
     CDS             24558..25358
                     /gene="nagB"
                     /locus_tag="OO515_001776"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6575726.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25418..26566
                     /gene="nagA"
                     /locus_tag="OO515_001777"
     CDS             25418..26566
                     /gene="nagA"
                     /locus_tag="OO515_001777"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6575727.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26575..27795
                     /gene="nagC"
                     /locus_tag="OO515_001778"
                     /gene_synonym="nagR"
     CDS             26575..27795
                     /gene="nagC"
                     /locus_tag="OO515_001778"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6575728.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27843..28595
                     /gene="nagD"
                     /locus_tag="OO515_001779"
     CDS             27843..28595
                     /gene="nagD"
                     /locus_tag="OO515_001779"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6575729.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6743 a         7343 c         7545 g         7146 t
ORIGIN      
        1 ctccgccggg gcacaccgaa caggccacac cggtgggggc accgatgcgc accccggctg
       61 aaccctggac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgcc gccggttttc
      121 cgctcatttc tcatccttgt tttatcgggt tatttgtccg ctgacaggca agaccctaca
      181 gatttataag aaagccttaa gtgagatatc cgtcagatag aaacaattag gaaaatattt
      241 atatatattt catgaatata tgtttcgatt ggtataacgg tgtctaaata ttcctctgaa
      301 catcttcctg atacaaaaaa ccgccagcgt ttccgctggc ggttttcaat gtattcagaa
      361 ctcagcaatc agtaaaaatc accggttgcc ttttcggcct gatccatcca caccggttta
      421 tcgctggttt tcgaccagac gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag
      481 aaaagcatga aaggcagcgc cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag
      541 ataagccatg caggatattc tctgtagagt tccatacttt tctccccctt ttgtatctac
      601 ccggtgaata gcaccggaaa aatgaatttg tttatctgat gaaaatagta ccgccttttg
      661 tgtaatttta ctactcatcc gaccacttat ttttgcttat tgatggttta tttacattca
      721 ctctgtaatt aagttacaca aaagttaaat taatactaaa cattagttaa atcatggctt
      781 ttgccatttt tatacttttt ttacaccccg cccgcagatt tttgcgaaat ctttgcagcc
      841 agaattctac tcttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg
      901 tgatagccgg cgtgttgctg gtgtttttat tactgggtta tttggtttat gccctgatca
      961 atgcggaggc gttctgatgg ctgcgcaagg gttcttactg attgccacgt ttttactggt
     1021 attaatggtg ctggcgcgtc ctttaggcag cgggctggcg cggctgatta atgacattcc
     1081 tcttccaggt acagcgggcg ttgaacgtat actctttcgt ttacctggcg tctctgacca
     1141 tgagatgaac tggaagcaat atctttgtgc cattctcggc ctgaacatgc tggggctggc
     1201 ggtgctgttt tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc
     1261 ggggctgtcg tgggatctgg cgctgaatac cgccgtcagc tttgtcacga ataccaactg
     1321 gcaatcttat agcggtgaaa ccacgttgag ctatttcagc cagatggcag gcttaacggt
     1381 acaaaacttt ctttctgccg ccagcgggat tgcggtgatt tttgccttca tccgtgcgtt
     1441 tactcgccag agcatgagca cactcgggaa tgcctgggtc gatctgctac gcatcacgtt
     1501 atgggtgcta gtccctgtgg cgttgttgat cgcactgttt tttattcaac aaggtgcgct
     1561 gcaaaacttt ctgccctatc aggcagtgaa taccgttgaa ggagcgcaac agctgttacc
     1621 catgggacca gtagcttctc aggaagcgat caagatgctc ggtactaacg gcggtggctt
     1681 ctttaatgcc aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca
     1741 gatgctggcg atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtgacggg
     1801 cgatcgccgc caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt
     1861 aggcgtggtt atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcaga
     1921 cagcagcatc aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt
     1981 tgcggtcgtg actacggcgg cgtcctgtgg cgcggtgatt gcgatgcatg attcgtttac
     2041 cgctctcggc ggcatggtgc cgatgtggct aatgcaaatt ggtgaagtgg tgttcggcgg
     2101 tgtcggttct ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg
     2161 gctgatgatt ggtcgtacac cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa
     2221 actgactgca ctggcgattc tggttactcc aacactggta ctgatgggcg cggcgttggc
     2281 gatgatgacc gacgccggac gtagcgccat gcttaaccct ggcccgcatg gttttagcga
     2341 agtgctgtac gccgtgtcgt ccgccgctaa caacaacggc agcgcctttg ccggattaag
     2401 cgccaactct ccgttctgga actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg
     2461 ggtgattatc ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc
     2521 cagctccggc acgctgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt
     2581 gttgctggtt ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata
     2641 tctctcctga tgatattgag cgagcactga atatgagtcg taaacaactg gcgctattcg
     2701 aaccaacact tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc
     2761 aatggcgcaa tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta
     2821 gcatcgcgat ggcaagcggt gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg
     2881 gctggctgtg ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca
     2941 gtaaagcgca ggccaacagt cttaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc
     3001 gtgagccgaa atatggcgct gcggcggaca aagttcctgc cgatcaactg cgtaaaggtg
     3061 acatcgtgct ggtagaagct ggcgatatta tcccctgcga tggtgaagtc attgaaggcg
     3121 gcgcatcggt cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg
     3181 gcggcgattt tgcctccgtc accggcggca cgcgtattct ttctgactgg ctggtgattg
     3241 agtgtagcgt taaccccggt gagacatttc tggatcggat gatcgcgatg gtggaaggcg
     3301 cacagcgacg caaaacaccg aacgagattg ccctgactat cctgctgatt gctctgacca
     3361 tcgtcttttt actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgccg
     3421 tgagtgtaac ggtactggtg gcgctgctgg tctgtctgat cccaaccact attggcggcc
     3481 tgttgtcagc gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtgattgcca
     3541 ccagcggacg tgcagttgaa gcggcaggtg acgttgacgt tctgctactg gataaaaccg
     3601 gcaccatcac actcggtaac cgtcaggcgt cggagtttat ccccgcgcag ggcgtggatg
     3661 aaaaaacgct ggctgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggtc
     3721 gcagtattgt gatcctcgcc aagcaacgtt ttaacctgcg cgagcgcgat gtgcagtcgc
     3781 tccatgccac ctttgtaccg tttactgcgc aaagccggat gagcgggatc aacatcgaca
     3841 accgcatgat ccgtaaaggt tctgtcgatg ccattcgccg ccatattgag gctaacggcg
     3901 gtcacttccc tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcag ggagccacgc
     3961 cgctggtggt ggtggaaggt tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca
     4021 aaggcggtat taaagagcgc ttcgcccagc tgcgcaaaat gggcattaaa acggtgatga
     4081 ttaccggcga taaccgtctg actgccgccg cgatagctgc ggaagcgggt gtcgatgatt
     4141 ttctcgccga agcaacaccg gaggccaagc tggcattgat ccgtcagtat caggcggaag
     4201 gtcgtttggt agcgatgacc ggcgacggca ccaacgatgc tccggcgctg gcgcaggcag
     4261 atgtcgcggt ggcgatgaac tccggcaccc aggcggcgaa agaggcgggc aatatggtgg
     4321 atctcgactc taacccgacc aagttgatcg aggtggtgca cattggcaaa cagatgctga
     4381 tgacccgtgg ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca
     4441 ttattccggc ggcgttcgcg gcaacatatc cgcagttaaa tgcgctgaac atcatgcgcc
     4501 tgcattcgcc cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct
     4561 ttttgattcc tctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt
     4621 tgcgccgtaa cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca
     4681 aagtcattga tttactgctg accgtttgcg gtctggtgtg aggtttacca tgagtggatt
     4741 acgtccggca ttatcgacat ttctctttct gttattgatt actggcggcg tttacccgct
     4801 gctgaccacc gcactggggc aatggtggtt tccctggcag gccaacggtt cgttgatccg
     4861 tgaaggtgat acggtgcgtg gctcggcatt aatcgggcag aattttaccg gcaacggcta
     4921 ttttcatggt cgcccgtcag caacggctga aatgccctat aatccacagg cttctggcgg
     4981 gagcaatctg gcggtcagta accctgagct ggataaacta atagccgcac gcgttgctgc
     5041 attacgggcc gctaacccgg atgccagcgc gagcattccg gttgaactgg tgacggcatc
     5101 ggcaagcggg ctggacaata atatcacccc gcaagcggcg gcctggcaga tcccacgcat
     5161 cgcgaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca
     5221 acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc
     5281 gctggataag cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac
     5341 aaactgccgc gccgcatcgg gggaagctga aagttttctt cggtgcctgt gcaggcgtcg
     5401 ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg
     5461 tggttggcgt ggtagaaacc cacgggcgaa aagataccgc cgcaatgctg gaagggctgg
     5521 ctgttctgcc gccaaaacgc ctggcgtacc gtgggcggca catctgcgag tttgacctcg
     5581 atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg
     5641 cgccaggttc tcgtcaccct aaacgctggc aggacatcga agagctgctg gaagcaggca
     5701 ttgatgtttt cactaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg
     5761 gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg
     5821 acgtggttct ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag
     5881 tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga
     5941 tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg
     6001 cctggcgggg gcatcctagc gaagagaaag tctggcacac gcgcgacgcg atccttttat
     6061 gcatcggcca taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg ctggcgtcac
     6121 ggctgggtag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg
     6181 agaaaaaacg tcgtgcaatt ctcagcgcct tacgtctggc gcaggaactg ggcgcggaga
     6241 cggcaacact ttctgatcca gcggaagaga aagcggtagt gcgttatgcc cgtgaacata
     6301 atctcggcaa gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt
     6361 ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg
     6421 aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa gacaagtggc
     6481 gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg
     6541 ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg
     6601 tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg
     6661 tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc
     6721 aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg
     6781 ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat
     6841 atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg
     6901 aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg
     6961 gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc
     7021 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
     7081 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc
     7141 cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc
     7201 tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga
     7261 tggcaagcga acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac
     7321 gcacgccgct tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg
     7381 aaggatcacc ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc
     7441 gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga
     7501 aagagtggtt aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt
     7561 tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc
     7621 cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc
     7681 aggccgaaat tggtatcgat gcccactttg agggcgaaaa tctacaactg gatgtctggg
     7741 ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg
     7801 ggaataaaga gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg
     7861 tggatgtaca cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc
     7921 gtgttacact tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac
     7981 aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga
     8041 gggcgacggg atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc
     8101 aacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga
     8161 gtttatccgc gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag
     8221 cgaagagagc gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc
     8281 gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac
     8341 cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt
     8401 gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt
     8461 gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg
     8521 gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa
     8581 actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta ttggatatcg
     8641 gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa
     8701 acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga
     8761 aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt
     8821 tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg
     8881 gatagaataa ctctaccttc aactgacaca gcaagaggta aaggtaaatg gaaaataata
     8941 accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc catcgtaata
     9001 gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta
     9061 caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac
     9121 tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac
     9181 gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga
     9241 tttatttatc agcggtggag ctttgctttt tttccggcgt gatcgatttc tcctttgaga
     9301 aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt
     9361 gccgttagcc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac
     9421 gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga
     9481 aaactcgatg aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
     9541 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
     9601 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta tgaaacgcaa
     9661 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
     9721 gactgcccgg gtcatcaggg aggtgagttt ttccgtcgtc atccggcagg gaatcagttc
     9781 gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga cgtagcaatg
     9841 ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
     9901 tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt
     9961 ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa ccacaaatct
    10021 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aacggcacgt
    10081 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
    10141 gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt ccgcctcgct
    10201 gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
    10261 attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta tgagcaattt
    10321 atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa cgatccgggt
    10381 attctggtta cgcaatccgt acataaacag caggctggtt tttctcaaac ttcacaaatc
    10441 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
    10501 aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
    10561 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
    10621 aatggcatta atgcccgcaa actgatcctc gataactgtc agcatattcg tccgttcgta
    10681 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
    10741 ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg ctatgcagag
    10801 aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
    10861 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgcgaa
    10921 aatggcgtag tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca
    10981 gaagatatgg ccaaacttca gcaacttgtt gccctgctgg tacgcttcga aaaactgctt
    11041 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11101 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11161 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11221 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11281 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11341 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11401 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11461 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11521 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11581 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11641 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11701 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11761 atgccgagta tgctttcggt aaatccggta actttatggc gaactatacc tacggcgtct
    11821 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11881 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    11941 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12001 ggggggtcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12061 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12121 ccatcgctat gacgctgtgg gcttttctcg gtctggagtc tgcgtgtgcg aatactgatg
    12181 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12241 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12301 caaattcaac ggcaccattt ggcctggcct tcgcacagat gttcacacca gaagtgggta
    12361 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12421 ccattgccca ggtgtttaag tcttcatctg atgaaggcta cttccctaaa attttctccc
    12481 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12541 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12601 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12661 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12721 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12781 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12841 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gccctttttt
    12901 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    12961 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13021 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccgat
    13081 agaagcacca ttgcccggtg ccgccgtcag gcgcgcggtg atcgggtcgc ctgccagggt
    13141 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13201 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13261 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acatgatgat
    13321 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13381 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13441 taccgggact tctaccagct tgcggcccaa gtcattgacc acacggtcga tcatcgcaga
    13501 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13561 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13621 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcaatgc
    13681 cagcaggccc gccatcgcac actcggagga gcagtccata cggatcgcgc cgtctttatc
    13741 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13801 gtaatactcg ccaattcgct tccagtattc gataccggaa ccacccagcg gatcaacgcc
    13861 cagcgtcagg cccgttttct gaatcgccgc catatcaacg atatcggcca gaccttccac
    13921 aaactgctgc accagatcct gctctttcac atgaccggat gccatcgctt cgtcgaggga
    13981 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggcacgat cttccaccac
    14041 tttagtgacg ttggtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14101 ttccggcggg ttatgggagg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14161 attatgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14221 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14281 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14341 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14401 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14461 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14521 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14581 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14641 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14701 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14761 caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14821 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14881 cgtattcgtc agaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    14941 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15001 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15061 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15121 tcatcttaat ccagtacagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15181 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15241 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15301 actttgccat gaaattgaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15361 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15421 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15481 gagatccggt aatgaattac ccggcgatgg cgcaggatct tgttgatact ctggatgcac
    15541 agcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15601 ttacggcact ggcccgcgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15661 actatcacgt acgccgtcat gatgagattt tcgcggctat caacgcggtc agtgaatcgg
    15721 acgcacaaac tcgccagcaa gcagcagcaa taatgcacca gcatcttaat gaagaagggg
    15781 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15841 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15901 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    15961 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16021 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16081 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16141 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16201 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16261 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16321 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16381 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg agtcgcgcaa tatgagccgt
    16441 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16501 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16561 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16621 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16681 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16741 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttatgaca ttctgctgct
    16801 gggcatccca acctggtatt atggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16861 tctcgaagag attgatttca acggcaaact ggttgcgctg tttggttgtg gtgaccagga
    16921 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    16981 cggtgcaacg atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17041 tctggctgat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17101 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17161 aattctcaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17221 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17281 cggtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17341 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17401 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17461 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17521 tttaaaaatc ctagaagttc ttcaggaacc ggacaaccat cacgtcagtg cggaagattt
    17581 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17641 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17701 atttgaactg acacagcaac atcaccacga tcacctgatc tgcctcgact gcggcaaggt
    17761 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17821 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17881 cgaagatgaa catgcgcacg aaggcaaata agccagcctg aatgagaaaa agccaacctg
    17941 cgggttggct tttttatgca agggaaataa gaaaagcgag agttacagct ctcacttatt
    18001 tgttattact gcgaatttcc tgccaaactt tatcacaatc ggctttcacc gcctgatcat
    18061 ttccggtttg cgttgcacgc agatactgct gataatccag tacgcggaca ctttcctggt
    18121 cggcaaaatg ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt
    18181 caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc tctttcagac
    18241 gactgtcttc ctgtggcgcg gttgattgcg cacaagctac cagccccgat gccattatgg
    18301 cgatgagaat cagttttttc atagcaaatt cctcaaacgt gcggcaggca caaaacctgc
    18361 cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca
    18421 tcctggaaga tgttgccgta accaccgccc cagcttggga tgtcggagtg gttgtcgtat
    18481 tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc
    18541 gcatccaggc tgtatgcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga
    18601 ttgctctgct aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg
    18661 aagcctggaa gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg
    18721 ccgttagcgt tgaagtcgga acggttatcc caccagatat ccaggcggcc gttggaggaa
    18781 gcgtaggttg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc cttaacccag
    18841 gtgccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc
    18901 agccaggcgg tgccgtcata aaggtcgttg acgctgcgat cgtcaacttt atcgcgcgta
    18961 ccatagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct
    19021 ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag
    19081 ttatttttga agtcgtattt cgccccaagg gagtgcagat aatcaacttt ggtggtttta
    19141 tcgttctgat aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac
    19201 atgtaggaga aactcaacgc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc
    19261 ccctgataag tacctggcat aaagctccag tgcggcgcta acagcgtttg accagttggc
    19321 tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac
    19381 aggcttatac cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa
    19441 aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac
    19501 gcagcaatat caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca
    19561 ttccaggtgg agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg
    19621 tcgcgttcac gctgccagta atatataccg ccggttaagg ttgaatcgtc gatgaatcct
    19681 tcggcgcgag cctccggcat tgccataaag cccgacattg ctgtaacacc ggcgatagcc
    19741 agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat
    19801 aaattgctgc accaaaggtg aacagcgaaa aaataaaaat taaaaaagta gcgttgagaa
    19861 aagtctcgct gactacagga acagactatt tttcgcgacg cgaattatca atctctttct
    19921 gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc
    19981 ttaagtttgc ggttgaaagg cttgcagaag aataaaagcg tgaaattcca gcaagatgaa
    20041 agcggttaca ttttatttaa ttatgttttg ttattttaaa tttattttat ggataaattg
    20101 cgaagcggaa ttaattcgcg aagaaaagca gaaaaaacgc cgctgaacgc ggcgttttgg
    20161 attgtttgca tatttcttgc cggatgcggc gtgaacgcct taaccggcct acaaaagcac
    20221 gcaaactcaa tatattgcag agatcatgta ggcctgataa gcgtagtgca tcaggcaatt
    20281 tagcgtttga aactgtttgt ctgttccttg ccggatgcga cgtaaacgcc ccatccggca
    20341 tagcgaaact taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg
    20401 gttaaatacc ggtttttccg ccgtagaatg gcggctatcg aggcagaagt aaccttcacg
    20461 ctcaaactgg aatgctttac ccgcaaccgc atctttcagc gacggttcag caaagccctg
    20521 tttgatcacc agcgattccg ggttaatcac cgacaggaaa tcatccgcag caccagggtt
    20581 cggcacgctg aacagacggt cgtacagacg gatttcaacc ggcagcgcat gtgccgcgct
    20641 cacccagtga ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc
    20701 ggcgtcataa gtacagaaga tggtggtgat attgccttcg gcatctttct ccacgcgttc
    20761 tgccttaatg acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta
    20821 ctgcttgtta gcttcttcgc ggaaatcggc gcgatcgatc caaatctcac cgctaaacgg
    20881 cacctggcgg ctacccattt ccggtttgtt cggatggttc ggcatggtaa ccatttcgcc
    20941 ttcgccctga tagttttcga taaccagttt caccggatcg ataaccgcca ttgcgcgcgg
    21001 ggcgttttcg ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt
    21061 gtcctgcttg gtcacgccga tgcgtttgca gaactcacga atagaagcag cagtgtaacc
    21121 acgacgacgc agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt
    21181 gtcggtcacc agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg
    21241 cgagaattcg tactggcgcg ggtgaacagg aatggtgatg ttgtccagca cccagtcgta
    21301 caggcgacgg ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagggc
    21361 atcgctgatg cagtgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg
    21421 gtggtgttca gcaaatttaa tgcggtacag caccggatcg cgcatcacga taaacggtga
    21481 agccatgtcg attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat
    21541 tttttcgaac agcgccaggt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc
    21601 cggttgcgtc agggtgccgc ggtattcgcg gatctgttcc ggcgtcagtt catcaacgta
    21661 cgccaggcct ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga
    21721 ggagtaacgg acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga
    21781 ctcaacatac tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg
    21841 gcctttatag tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg
    21901 cagatagcca ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc
    21961 cagatcttca tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat
    22021 cgtggattcc tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg
    22081 ctgacaactg ttagttagcg aaaatcgaaa atctcactga gattatgggg atgattgatg
    22141 aaaaaaaaag cggcagagaa gatctccacc gcttttgcca gtcccgcgtc attgttggat
    22201 gcgcgtctta tccaaccagg actgagcttt tgtaggcctg ataagacgcg tcaagcgtcg
    22261 catcaggcat aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac
    22321 attgccctga gctttaatga tcaagccact gaaatcgtcg atattgctgc aaaccaccgg
    22381 gctaatcatc gaacgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg
    22441 ccctgcgctc acctgcgcgc cctcttccac cagacgttta aagcctttac cttccagcgc
    22501 tacggtgtcg atacccatgt ggacgacaat ctccgcgcct ttttcggttt ctaggcagaa
    22561 cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt
    22621 atctgtcggt ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcaga
    22681 aacctgatcc agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac
    22741 cgcgtttggt acagcctgcg gttttgctac aggagcggca gttgccggag ttgcttcagc
    22801 tgacgcagct gctaccgggc cacgggcaac gactttcttc atcgcatcgc caatggattc
    22861 tgctttcgcg ccaacaatca cctgaatggt ctgtttgttc agttttacta caccagaagc
    22921 acccagacgt ttacacatcg catcgttgac gcgagctgag tcagccacgg taaggcgcag
    22981 acgggtaata caggcgtcaa tcgctttcag gttgtcagta ccgccaaccg cagcaatata
    23041 gttggttgcc agttgagtca gaccttcttc agtattgctg ttagcctctt cagttacgat
    23101 ctcgtcttct ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact
    23161 gaacaccacg aagtagatag cgaagaagac aacacccatc accagcagca tccagacgtt
    23221 ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg atagcccccg cagagaaaga
    23281 gaagccagca tggataccca gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc
    23341 gtgcaggagg tacagcagcg gagcaaggaa catgaacagg aattccagcg gctcagtcac
    23401 accggtcagg aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg
    23461 ctctttcggt gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcatgat
    23521 cgggaagaag ccggacatga acatccccgc ggtgccgtca ccagcgtaga aacggttgat
    23581 gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat
    23641 ggtgttcagc acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat
    23701 accggaaccc agcgcgcctg cagaaacgat ccactcgccg cctgcatgga tagcgtgctg
    23761 tactggcggc cagacgtaac caaaaatggc cgccagcacc aggcagaaaa agccggtggc
    23821 aatcggcaca aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt taatatcgga
    23881 ccaacggtta taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat
    23941 gttaatttct gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc
    24001 cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa
    24061 gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg caacgttaag
    24121 taaatctggc tgaccgaatc gcagcaacag tgccgccacc ggcagcaccg cgatagggag
    24181 ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg
    24241 agaaccctat ttggctcgtt taaagccgta tttttatttt gctgcaaatt gtactgccga
    24301 tgttctgtaa tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgtat
    24361 cgcaaattaa acgcgtgcct tttgtgagtt ttgtcaccaa atatcgttat catcactccc
    24421 ttttactggc taaagcagaa aacttatttt atcattcaaa aaatcaggtc ggattgacgc
    24481 ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg cttacatttt
    24541 acttattgag gtgaataatg agactgatcc ccctaactac cgctgaacag gtcggcaaat
    24601 gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa accgactgcc gatcgtccgt
    24661 ttgttctggg cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa
    24721 tgcataaagc aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg
    24781 tcggtctgcc gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg
    24841 atcacgttga tattccagca gaaaacatca accttctcaa cggcaacgcc ccggatatcg
    24901 acgccgaatg ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta
    24961 tgggcggtgt aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt
    25021 ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt cgcgaactct cgtttctttg
    25081 ataacgatgt caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acattgctgg
    25141 atgccgaaga agtgatgatt ctggtgctgg gtagccagaa agcactggca ctgcaggctg
    25201 cagttgaagg ttgcgtgaac cacatgtgga ccatcagctg tctgcaactg catccaaaag
    25261 cgatcatggt gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt
    25321 tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggctcc
    25381 ttgctcaggg caatattttt taaaatcggg gggcagaatg tatgcattaa cccagggccg
    25441 gatctttacc ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat
    25501 taaaagcgtc tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg
    25561 ggccattctc tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt
    25621 taacgacacc gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga
    25681 gaaatcaggc tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa
    25741 acagggcgtg cgcgttatgc gcgagtacct ggcaagacat ccgaatcagg cgttaggtct
    25801 gcatctggaa ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt
    25861 gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa
    25921 agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg
    25981 gattgtggtt tctgccggtc actccaacgc gacgttgaaa gaagcgaaag ccggtttccg
    26041 cgcggggatt acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga
    26101 accgggcctg gcgggcgcga tcctcgacga agctgacatt tattgcggta ttatcgctga
    26161 tggcctgcat gttgattacg ccaacattcg taacgctaaa cgccttaaag gcgacaaact
    26221 gtgtctggtt accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc
    26281 gggtaaaaca atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg
    26341 ttcatcctta accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact
    26401 ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg
    26461 tctcggcaca ctcgccgcag gtaaagtagc caacctgacc tcattcacac ctgattttaa
    26521 aatcaccaag accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca
    26581 ccaggcggac aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg
    26641 gtttatcgcc tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc
    26701 cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc
    26761 aaagaagttg atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa
    26821 acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc acgacgccac catcaccctg
    26881 tttgatctca gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag
    26941 caaacgctgg aacatgccct gttgaatgcc attgctcagt ttattgatag ctaccagcgc
    27001 aaactgcgcg agctgatcgc gatttcggtg atcctgccag ggcttgttga cccggacagc
    27061 ggcaaaattc attacatgcc gcatattcag gtagaaaact gggggctggt agaagctctg
    27121 gaagaacgtt ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg
    27181 gagcactact tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc
    27241 ggaaccgggg ccgggattat ctctaacggg cgcattttta ttggccgcaa cggtaacgtc
    27301 ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt
    27361 ggctgcctgg aaactatcgc cgccaacgct gccattgaac aacgggtgtt gaatctgtta
    27421 aagcagggct accagagccg cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa
    27481 gccgcgaaca aaggcgatag tctggcgtcg gaagtaattg agtatgtcgg tcgtcatctg
    27541 ggtaaaacca tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgccggt
    27601 gaaatcaccg aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccag
    27661 gcgctgaagg cgtttcgtac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg
    27721 gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat
    27781 ttgctggaaa attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt
    27841 ccatgaccat taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg
    27901 ccgtaccggg tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt
    27961 tgctgaccaa ctatccttcg cagaccgggc aagatctggc gaaccgcttt gccactgccg
    28021 gtgtcgatgt gccggatagc gtgttttata cctctgcgat ggcgaccgcc gatttcctgc
    28081 gtcgccagga aggcaagaaa gcatatgtgg tgggcgaagg cgcactgatt catgaactgt
    28141 acaaagccgg tttcactatt accgatgtga accctgattt cgtgattgtt ggcgaaacgc
    28201 gttcctacaa ctgggacatg atgcataaag cggcctattt cgtcgctaac ggtgcacgct
    28261 ttatcgccac caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt
    28321 gtgcagggat tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt
    28381 ggatcatccg cgcagcatta aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg
    28441 gcgataactt gcgcaccgac attctggcgg gcttccaggc aggtctggaa acgattctgg
    28501 tgctttctgg tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga
    28561 tttacccgtc agtcgctgaa atcgacgtta tctgaatacc acccacgtct gcggacgtgg
    28621 ttggctctga tgacaaacgc caaactgcct gatgcgctac gcttatcagg cctacgcagc
    28681 tcctgcaata tattgaattt tcatgctttt gtaggccgga taaggcgttc acgccgcatc
    28741 cggcatgaac aaatcgcact ttatcagcaa tttggcc
//
LOCUS       DAJTXQ010000042        28398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000042 DAJTXQ010000000
VERSION     DAJTXQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.042"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1046)
                     /locus_tag="OO515_001780"
     CDS             complement(<1..1046)
                     /locus_tag="OO515_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6575730.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OO515_001781"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OO515_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OO515_001782"
     CDS             complement(2433..3281)
                     /locus_tag="OO515_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6575731.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OO515_001783"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OO515_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6575732.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OO515_001784"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OO515_001784"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6575733.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO515_001785"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO515_001785"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6575734.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OO515_001786"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OO515_001786"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6575735.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OO515_001787"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OO515_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6575736.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OO515_001788"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OO515_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6575737.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OO515_001789"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OO515_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6575738.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OO515_001790"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OO515_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6575739.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO515_001791"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO515_001791"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6575740.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OO515_001792"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OO515_001792"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6575741.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OO515_001793"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OO515_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OO515_001794"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OO515_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OO515_001795"
     CDS             22669..23652
                     /locus_tag="OO515_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6575742.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAADDYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OO515_001796"
     CDS             23675..25186
                     /locus_tag="OO515_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6575743.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OO515_001797"
     CDS             25211..26209
                     /locus_tag="OO515_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6575744.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OO515_001798"
     CDS             26242..27336
                     /locus_tag="OO515_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6575745.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OO515_001799"
     CDS             27348..28220
                     /locus_tag="OO515_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6575746.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28398)
                     /locus_tag="OO515_001800"
     CDS             complement(28268..>28398)
                     /locus_tag="OO515_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6575747.1"
                     /translation="YWDMTGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6723 a         7016 c         7724 g         6935 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagagacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccga cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaat
//
LOCUS       DAJTXQ010000043        28355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000043 DAJTXQ010000000
VERSION     DAJTXQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.043"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..527)
                     /locus_tag="OO515_001801"
     CDS             complement(<1..527)
                     /locus_tag="OO515_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575748.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRG"
     gene            complement(630..1985)
                     /gene="melA"
                     /locus_tag="OO515_001802"
     CDS             complement(630..1985)
                     /gene="melA"
                     /locus_tag="OO515_001802"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6575749.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2268..3176
                     /gene="melR"
                     /locus_tag="OO515_001803"
     CDS             2268..3176
                     /gene="melR"
                     /locus_tag="OO515_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6575750.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3375..5642
                     /gene="adiA"
                     /locus_tag="OO515_001804"
     CDS             3375..5642
                     /gene="adiA"
                     /locus_tag="OO515_001804"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6575751.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGASGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            5967..6728
                     /gene="adiY"
                     /locus_tag="OO515_001805"
     CDS             5967..6728
                     /gene="adiY"
                     /locus_tag="OO515_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6575752.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            6865..8202
                     /gene="adiC"
                     /locus_tag="OO515_001806"
     CDS             6865..8202
                     /gene="adiC"
                     /locus_tag="OO515_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6575753.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8306..9949
                     /gene="eptA"
                     /locus_tag="OO515_001807"
     CDS             8306..9949
                     /gene="eptA"
                     /locus_tag="OO515_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6575754.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            9946..10614
                     /gene="basR"
                     /locus_tag="OO515_001808"
     CDS             9946..10614
                     /gene="basR"
                     /locus_tag="OO515_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6575755.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10615..11715
                     /gene="pmrB"
                     /locus_tag="OO515_001809"
     CDS             10615..11715
                     /gene="pmrB"
                     /locus_tag="OO515_001809"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6575756.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(11691..11780)
                     /gene="pmrR"
                     /locus_tag="OO515_001810"
     CDS             complement(11691..11780)
                     /gene="pmrR"
                     /locus_tag="OO515_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6575757.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(11892..13394)
                     /gene="proP"
                     /locus_tag="OO515_001811"
     CDS             complement(11892..13394)
                     /gene="proP"
                     /locus_tag="OO515_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6575758.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13658..14524)
                     /locus_tag="OO515_001812"
     CDS             complement(13658..14524)
                     /locus_tag="OO515_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6575759.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14521..16749)
                     /gene="crfC"
                     /locus_tag="OO515_001813"
     CDS             complement(14521..16749)
                     /gene="crfC"
                     /locus_tag="OO515_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6575760.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17162..17497
                     /locus_tag="OO515_001814"
     CDS             17162..17497
                     /locus_tag="OO515_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6575761.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17655..18098
                     /gene="yjdN"
                     /locus_tag="OO515_001815"
                     /pseudo
     CDS             17655..18098
                     /gene="yjdN"
                     /locus_tag="OO515_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18231..19019
                     /gene="phnC"
                     /locus_tag="OO515_001816"
     CDS             18231..19019
                     /gene="phnC"
                     /locus_tag="OO515_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6575762.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19044..20060
                     /gene="phnD"
                     /locus_tag="OO515_001817"
     CDS             19044..20060
                     /gene="phnD"
                     /locus_tag="OO515_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6575763.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20186..20965
                     /gene="phnE"
                     /locus_tag="OO515_001818"
     CDS             20186..20965
                     /gene="phnE"
                     /locus_tag="OO515_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6575764.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            20986..21711
                     /gene="phnF"
                     /locus_tag="OO515_001819"
     CDS             20986..21711
                     /gene="phnF"
                     /locus_tag="OO515_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6575765.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            21712..22164
                     /gene="phnG"
                     /locus_tag="OO515_001820"
     CDS             21712..22164
                     /gene="phnG"
                     /locus_tag="OO515_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6575766.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22161..22745
                     /gene="phnH"
                     /locus_tag="OO515_001821"
     CDS             22161..22745
                     /gene="phnH"
                     /locus_tag="OO515_001821"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6575767.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            22745..23809
                     /gene="phnI"
                     /locus_tag="OO515_001822"
     CDS             22745..23809
                     /gene="phnI"
                     /locus_tag="OO515_001822"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6575768.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            23802..24647
                     /gene="phnJ"
                     /locus_tag="OO515_001823"
     CDS             23802..24647
                     /gene="phnJ"
                     /locus_tag="OO515_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6575769.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24644..25402
                     /gene="phnK"
                     /locus_tag="OO515_001824"
     CDS             24644..25402
                     /gene="phnK"
                     /locus_tag="OO515_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6575770.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25513..26193
                     /gene="phnL"
                     /locus_tag="OO515_001825"
     CDS             25513..26193
                     /gene="phnL"
                     /locus_tag="OO515_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6575771.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26190..27326
                     /gene="phnM"
                     /locus_tag="OO515_001826"
     CDS             26190..27326
                     /gene="phnM"
                     /locus_tag="OO515_001826"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6575772.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27326..27883
                     /gene="phnN"
                     /locus_tag="OO515_001827"
     CDS             27326..27883
                     /gene="phnN"
                     /locus_tag="OO515_001827"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6575773.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            27870..28304
                     /gene="phnO"
                     /locus_tag="OO515_001828"
     CDS             27870..28304
                     /gene="phnO"
                     /locus_tag="OO515_001828"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6575774.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
BASE COUNT         6825 a         7899 c         7332 g         6299 t
ORIGIN      
        1 ccccgatcgc caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag
       61 cccgccagac tggcaaaaaa acgcggataa ggaaccagtt gttcacgctc acgtttatcg
      121 agcgtgatgg ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc
      181 cagaggatgt aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca
      241 ctaaagagga gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg
      301 aacttacccc atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata
      361 gcatcccaga tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccaacg
      421 acatcggtgt agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg
      481 aacgctccaa atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc
      541 gctgttacca acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat
      601 cccccggcgc agtagcgttt agtcgcgttt taacggtgca accagcctgg cagccagtcg
      661 ccgtgggcgg caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg
      721 gcagtatgcg ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata
      781 gcttcggtca gcagcgtctg tacattgatg ttggtttgca tcagggcggc cagatgcgaa
      841 ggtagcgtac cgactttagt cggctgaatg ccattagcat caaccagaca ggctacttcc
      901 acgcaacatc cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc
      961 acactcggct cgccagtcca gatagcgttc atgattgtgc tggcatattc tcgtgacggt
     1021 ttaatatcaa tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc
     1081 tgctcgacgc agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc
     1141 tcacgacctg gcttaataaa ccacggcgtg tattcagcaa aatgttctga cgattccgta
     1201 acgaagtagc ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta
     1261 ccatgaatat ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag
     1321 agattcacgt aactgccgtc ggcggtttta cgctccagtt ccaggtaaaa cgccatatgg
     1381 ttgataccag cgcaacggta acgcagcgtg gacggatcga tattgaggtc acgcgccagc
     1441 tcttccgccg ttccctgcac cgaatggcac agccctacct gtttgatatg cagatagcgg
     1501 gcatacatcg cccaggtatt catcgccatt gggttaacgt agttgagcat ggtggcatcg
     1561 gggcagactt ccgtcatgtc ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg
     1621 cgcataatac cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta
     1681 cagacctcga aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg
     1741 acaaaatcgg catcctgtaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg
     1801 gcccctgctg aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtggga
     1861 tcaatgtcca tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca
     1921 agaatatttt taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagtc
     1981 atcatgcaga tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa
     2041 acctccggct tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa
     2101 atctgagttt atggtaataa gttacctgaa aagcagagtt ttcggcctca tgatgctcgg
     2161 acgctcccct cgcctctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct
     2221 ccctcactcc tgccataatt ctgatattcc aggaaagaga gccatccatg aatacagata
     2281 cgtttatgtg cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat
     2341 accagcggat ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg
     2401 gtcaagtcga agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa
     2461 aagtgaatat caatcagggt aatatcacgc tgttctgggc ctgtacaccg caccaactaa
     2521 cagataccgg aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct
     2581 cctggccgct ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac
     2641 tggccacaca gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa ttaaacagtc
     2701 cgaacgagca aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta
     2761 gcctctctgg ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg
     2821 tctcgcgcca tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg
     2881 atcaggcgct gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa
     2941 tggggatatt ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca
     3001 tcaaccacgt tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga
     3061 cggcaggctt tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt
     3121 cgccgcaaca ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac
     3181 gatatcagta tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt
     3241 aaaataatca cataacgcat tgtttataaa aataaatatt tcaaaaatgt ttgtttttca
     3301 cgcgctttac agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa
     3361 ccgggtaagc aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct
     3421 gggtcggtaa cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga
     3481 ttaaatccac ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact
     3541 gcctgatgtt cagctatcaa atggaacatc cggacgaaca tcaaaacgtc agacaattga
     3601 tcggtaagct tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa
     3661 aagccctcgc cgcaatggat cgcgacctgc tggagcttgt cgatgaattc gcctggattc
     3721 tggaagatac cgccgacttt atcgccggac gcgctgttgc cgcgatgacc cgctaccgcc
     3781 agcagctgtt gccgccactg ttcagcgcgc tgatgaaata tagcgacatc catgaatatt
     3841 cctgggcagc gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt
     3901 tctaccatga ctactatggt gaaaatctgt tccgcaccga tatgggcatc gaacgaactt
     3961 ccctcggttc tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac
     4021 gcgtatttgg tgccgatcgc tcctggtcgg tagtcgtcgg tgcttccggc tctaaccgca
     4081 ccatcatgca ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata
     4141 aatccatcga acaaggcttg atgctgacag gcgcgaaacc ggtctacatg gtgccaagcc
     4201 gcaaccgcta cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc
     4261 agaagaaaat cagtgaaagc ccgctgacta aagacaaagc cgggcaaaaa ccgtcttact
     4321 gcgtggtgac caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc
     4381 tgctggaaaa aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt
     4441 tcaacccgat ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc
     4501 ctaccgtttt cgccacccac tccacccaca aactgctgaa tgcgctttca caggcttctt
     4561 atattcatgt acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca
     4621 tgatgcatgc caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt
     4681 cgatgatgga cggcaacagc ggcctgtcac tgacgcagga agtgatcgac gaagcggttg
     4741 atttccgtca ggcgatggcg cggctgtata aagagttcac cgctgacggt agctggttct
     4801 tcaaaccgtg gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg
     4861 ctgacgcacc aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg
     4921 aaagctggca cggcttcaaa gatattccgg ataactggag tatgctcgac ccgattaaag
     4981 tcagcatcct tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg
     5041 cggcgctggt cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact
     5101 tccaaattat gttcctgttc tctatgggcg taacccgtgg gaaatgggga actctggtta
     5161 acaccctttg ctccttcaaa cgccactatg acgccaacac accgctggcg caggtgatgc
     5221 cggaacttgt tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg
     5281 acaccatgtt tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt
     5341 ccggcctgtc ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca
     5401 atgtcgaact ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc
     5461 catatccgcc aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc
     5521 cgcaagtaag ttatttacgc tcgctgcaat cctgggacca ccatttccct ggatttgaac
     5581 acgaaactga agggactgaa attattgacg gtatttacca cgttatgtgt gtgaaagcgt
     5641 aaccactatt ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc
     5701 cttttgagta tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg
     5761 cgcggcccca ccccgcgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat
     5821 gagaaattac tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat
     5881 attcatcata caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc
     5941 ttttattcgc actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt
     6001 tattgactga aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag
     6061 ctaaccatat ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca
     6121 acactttggt tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata
     6181 aagatctctc gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat
     6241 gcctgacgcc agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg
     6301 agtcagaaaa ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca
     6361 gtaaaaaatt cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt
     6421 atcaaattat tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt
     6481 tatgtcttag cccaagtctg ttaaagaaaa agctgaaaag cgaaaacacc agttatagcc
     6541 aaataatcac cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa
     6601 atatctctca ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct
     6661 ttaaagactt ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgaactg
     6721 tcgcctgatt tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg
     6781 tcgataataa ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa
     6841 gctcgccttc gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta
     6901 atccccgtca ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct
     6961 gcaaacctgg cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt
     7021 gcgctggggc tctcgatggt atacgccaaa atgtcgttcc tcgatccaag tcctggtggt
     7081 tcttacgctt acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc
     7141 tactggctgg cctgctggat cggcaatatc gctatggtgg tcattggcgt aggatattta
     7201 agttacttct tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg
     7261 ctgtggatct tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg tgtgcaggcc
     7321 gttgccaccg tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg
     7381 ttccgtggtg aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg
     7441 attcaaagta cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt
     7501 gccgcaggtg tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta
     7561 ttgattgccg ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat
     7621 gccgcactgc gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac
     7681 accgctgggg ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc
     7741 tggacgttgc tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg
     7801 atttttgccc gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt
     7861 ttgatgacca tcttccagct cagtagcatt tcgccaaacg caaccaaaga gttcggtctg
     7921 gtatcttccg tgtcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta
     7981 ctgctgctcg gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc
     8041 attgccttcc tctactgcat ctgggccgtg gtggggtccg gggcgaaaga ggttatgtgg
     8101 tcatttgtca ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat
     8161 aaaaacccgt atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa
     8221 acataaaagc caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg
     8281 cactttgttc gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagcc cctctttgaa
     8341 tttactcgcc tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt
     8401 ttttaaacag gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt
     8461 gtcgatgccg gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt
     8521 atggcttaat cgaccactgg cctgcctgtt tattctggtt ggcgcggctg cacaatattt
     8581 cataatgact tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac
     8641 tccagcagaa agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg
     8701 cgtgcttgct gcgctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg
     8761 cagtgttctt ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc
     8821 cgcactgttt tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc
     8881 cttaagcccc tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc
     8941 aaatctaccg ctggtgcgaa ttggtgaaga cgcccaccgc aacccgttaa tgcagaacga
     9001 aaaacgtaaa aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc
     9061 cctcaacggc tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt
     9121 ccctaatacc gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga
     9181 tatgccgcgt gagcattaca aagaagagct ggcacagcac caggaaggcg tgctggatat
     9241 cattcagcaa gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc
     9301 ctgcgaccgc gtgcctcacc agaacgtcac cgcgctgaat ctgcctggtc agtgcatcaa
     9361 cggcgaatgc tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca
     9421 gggtgatggc gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg
     9481 ctatccgccg cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg
     9541 taccaaagag caactggtga acacttacga caacacgctg gtttacgtcg actatattgt
     9601 tgataaagcg attaatctgc tgaaagaaca tcaggataaa tttaccacca gcctggttta
     9661 tctttctgac cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta
     9721 tgccatcgcc ccggatagcc aaaaacaggt gccgatgctg ctgtggctgt cggaggatta
     9781 tcaaaaacgg tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta
     9841 ttcacaagac aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta
     9901 ccaggctgcg gatgatgttc tgcaaacttg caggagagtg agtgaatgaa aattctgatt
     9961 gttgaagacg atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac
    10021 acgtgcgatg gcgtaacaac cgcgcggatg gcggaacaaa gccttgaggc cggtcattac
    10081 agcctggtgg tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt
    10141 atccggcaga aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc
    10201 gacaaaatcg ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg
    10261 gaagagttac acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt
    10321 gagctgattg ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt
    10381 gaagagttga ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca
    10441 ggcagtccgg tacatcggga aattctctac aacgacatct ataactggga caatgaaccc
    10501 tcgaccaaca ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt
    10561 atccgcaccg tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat
    10621 ctgatgcgtt ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg
    10681 gccattttgt tggtgtttga gctgatcagc atcttctggc tatggcatga aagtaccgag
    10741 cagattcagc tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg
    10801 cgtgagatcc gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg
    10861 acgctattta tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa
    10921 aaagagctgg aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc
    10981 ctcgaaatcg aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gacgagcacg
    11041 ctggataacg aaaggttatt taccgctgac gtcgcgcacg aactgcgaac gccactggcg
    11101 ggggtgcgtt tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta
    11161 gtggcacggc ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc
    11221 ggacagtcat tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc
    11281 ccctcgtatg acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca
    11341 gagagcgccg ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga
    11401 aacctggtag aaaacgccca tcgttacagc ccacaaggca gcaacattat gattaagctg
    11461 caagaagatg gcggagcggt catggccgtt gaagatgaag gaccgggtat tgatgagagt
    11521 aaatgcgggg agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt
    11581 ctggggttaa gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa
    11641 aaccggcaag agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagtacgta
    11701 gcaaaccaga tataaagaaa gctgctgacc accagcacac tgaacacggt agttaaactt
    11761 tcataaacac ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc
    11821 agagattaag tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca
    11881 tccgtttcag cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc
    11941 tgcaaatcgg caatctcgtg gtcaatatca tcgattttct gctcgatatt atcgtaatgc
    12001 tcgacgagaa tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac
    12061 ggacgatttg ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc
    12121 atcaggtaat aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt
    12181 gttggcgtca gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa
    12241 cggatatgcg tcgggaacat cgctggcaag gtagaagcca taacgcccgt aaagcagtta
    12301 aggatcaccg ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc
    12361 agaataaacg ccgggatcgc caacacaaac agggcaacac taccaagtag cacaaacgga
    12421 cgacggccaa aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc
    12481 ataatggcga taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa
    12541 taactcggca tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca
    12601 atacatgtca acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg
    12661 ccatcctgca aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc
    12721 ggagtttctt ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata
    12781 aagaacggaa tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt
    12841 aacaccacca cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg
    12901 cccataaagc cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta
    12961 tattcaccgc cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc
    13021 caaataccaa tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata
    13081 atcacaatag tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag
    13141 aagagcccgc caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc
    13201 atctgcacgc tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa
    13261 ccataaacac caaaatcgaa ccattccatt gcattaccca gtgatgctgc ggtaatggct
    13321 ttacgcagtt taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt
    13381 ttccttttca gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat
    13441 cagcgcgacg gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt
    13501 agcgggtttg cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa
    13561 ttgggcagtt aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca
    13621 tggatttcgt ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga
    13681 tttcctcgac cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg
    13741 cgactgtttg ccagcgttgg gtcactgacc agcaaacggg ctgtttatct gccgtggacc
    13801 actttcccag ccaacttaaa aaatcagcat gctcccctcc cgaagaggca aaccactgat
    13861 ggtaatagtg acgcgtggtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    13921 tctcgcgcaa atcacccagt aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    13981 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    14041 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    14101 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    14161 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    14221 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    14281 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    14341 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    14401 cacgaaactg ctgctgatgg gcttgtggaa ggcgggcatc atcgccctgc gccagatcca
    14461 ctaaaaagcg gggataaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    14521 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gggcgtcggt
    14581 gtaaatccag ccgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    14641 cgattcattt tgttgacgca gagccaggct ttcgcgtaaa cgcgtctgta attccgtcaa
    14701 acatgaagcg aactcagcgc aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    14761 ctctgccaga actttagcca tttgttggca ataaagatcg acttctgcgc ttaaagctcg
    14821 ttgcaacaca ctgtaatcaa ccgtctctgt cggggatttc tcatttcccc gtccccagtc
    14881 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    14941 gtccgttgca tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgacgtgggg
    15001 aactactccg tgaaaaacag gaaaatgaac cccaggacga aaccctgact cgctcaattc
    15061 cattttgact tgttgctcaa tgggccgaat aacatcggtt aacacccggc aaagggtgga
    15121 ttcaagctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    15181 cagtgtctca cagcaggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    15241 ggctaactgc gcattcagcg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    15301 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    15361 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    15421 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    15481 cgcagcagat cgcagagcat acaacgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    15541 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    15601 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    15661 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catggcgcgc
    15721 tcgatttgcc aagtagcccc acatcgacga caccggaaat atctgctgtg gcgtaataca
    15781 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    15841 atcctgttga tcgaacttat tgaccagcac atacagtggc accgattgcc ccaccgccaa
    15901 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    15961 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    16021 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tctccagccc
    16081 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    16141 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    16201 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    16261 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    16321 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    16381 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    16441 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    16501 tgatttccct gctttcatag taccgacaat cgccagcacc atttccagtc gggaaatttt
    16561 actcaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    16621 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    16681 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    16741 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    16801 ttttgagttc tgtttgagtt ctgtttttgg cttttataat tactgcaaga aataatttta
    16861 tatttagtgt gttgtttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca
    16921 gaaagataaa aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta
    16981 aataataaat agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga
    17041 tcgtcgtcta aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc
    17101 ctttttcggc aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca
    17161 catgtcatta ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat
    17221 gtacatctgc ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga
    17281 cgagctgatc gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat
    17341 cattaaagat ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa
    17401 aaacatccgc ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc
    17461 gatgaaactg aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg
    17521 ataaggcgct cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt
    17581 cttatcaggc agcctctcat ctgtataaat ttcgaactac acttaattgg cttctcttaa
    17641 ctgaggtcac catcatgccg ttaagtccct acctctcttt tgccggtaac tgtgccgacg
    17701 caattgctta ttatcaacgt acgttgggcg ctgaactgct ctataaaatc agcttcggcg
    17761 aaatgccaaa atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg
    17821 ataccgccat cgcccacgcc aatgtgcgca ttgccggaag cgacatcatg atgagcgatg
    17881 ccatgccgtc aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg
    17941 tcgaagaagg aaaacgctgg tttgacaatc ttgccgctaa cggaaaaatc gaaatggcct
    18001 ggcaggaaac tttctaggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt
    18061 ggatgattaa tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc
    18121 cgcactgtca tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat
    18181 cgtcacaata atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga
    18241 ttatccgtgt cgagaagctc gccaaaacct tcaatcagca tcaggcgctg catgcggttg
    18301 ctctgaacat tcatcacggt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat
    18361 ccaccctttt acgtcactta agcggtttga ttaccggcga taaatccgcc ggcagccata
    18421 tcgagctgct gggccgcaca gtccagcgcg aaggccgtct ggcgcgcgat atccgcaaaa
    18481 gccgcgccaa caccggctac atcttccaac aattcaacct ggtgaaccgc ctgagcgtac
    18541 tggagaacgt gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct
    18601 ggtttacccg cgagcagaaa caacgcgcgt tacaggcgct gacccgcgtt ggcatggtgc
    18661 attttgccca tcaacgcgtt tccaccctct ccggcggaca gcagcagcgt gtggcgattg
    18721 cccgcgcgct gatgcagcag gcgaaggtga ttctggccga tgaacccatc gcctcgctgg
    18781 acccggaatc cgcccgcatc gtgatggaca ccctgcgcga catcaatcag aacgacggca
    18841 tcaccgtggt cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg
    18901 tcgccctgcg ccaggggcac gttttctacg acggcagcag ccaacagttt gataacgaac
    18961 gttttgacca tctctaccgc agcattaatc gcatcgaaga gaacgcgaaa gctgcctgac
    19021 atccccatca ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca
    19081 gcatgttcag cctcagcacc ctgttaagcc cggcacacgc cgaagagcag gaaaaggcgc
    19141 tgaatttcgg cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccat
    19201 tcttacagga tatggagaag aagctgggcg tgaaggtgaa cgccttcttt gccccagact
    19261 acgcaggcat tatccaggga atgcgcttca ataaagtgga tatcgcctgg tacggcaacc
    19321 tgtcggcaat ggaagcggtg gatcgcgcca acggccaggt cttcgcccag acggtcgcgg
    19381 cggatggatc gccaggttac tggagcgtgt tgatcgtcaa caaagatagt ccgatcaaca
    19441 acctgaacga tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact
    19501 ccacctctgg cttcctcgtc cccggttact acgtctttgc caaaaacaat atctccgcca
    19561 gcgactttaa gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca
    19621 agcaggtgga tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc
    19681 cagagaagct gaaagaactg aaggtgatct ggaagtcgcc gctgatccca ggcgatccga
    19741 tcgtctggcg caagaatctc tccgaaacca ccaaagacaa gatctacgac ttctttatga
    19801 actacggaaa aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcaccgtttc
    19861 gcgcctccag cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagattc
    19921 agggcgtaaa aagcaataac ggactgaatg agcaggacaa gctggcgaaa accaccgaga
    19981 ttcaggcgca gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg
    20041 tgagtaaagc ggtgcagtaa tgtgctcgat cggtcccctc tcccttgagg gagagggtta
    20101 gggtgagggg gaaatgcgca cgctgatgcc ctcaccccgg ccctctccca cagggagagg
    20161 gagaaaacat taaacggagc caaacatgca aaccatcacc atcgccccac ccaagcgcag
    20221 ctggttctcg cttctgagct gggccattgt tctcgccgtg ttggtcgtct cgtggcaggg
    20281 cgcggaaatg gccccgctta cgctgatcaa agacggcggc aacatggcga ccttcgccgc
    20341 cgacttcttc ccgcccgatt tcagccagtg gcaggattac ctcaccgaaa tggccgtcac
    20401 gctgcaaatc gccgtctggg gcaccgcgct ggcagtggtt ctctccatcc ccttcggcct
    20461 gatgagcgcc gaaaacctgg tgccgtggtg ggtttaccag cccgttcgcc gcctgatgga
    20521 cgcctgccgc gccattaacg aaatggtctt cgccatgctg ttcgtggtcg ccgtcggtct
    20581 cggaccgttc gctggcgtgc tggcgctatt tattcacacc accggcgtgc tctcgaaact
    20641 gctttccgaa gcggtggaag cgattgaacc cggcccggtg gaaggcattc gcgccaccgg
    20701 cgccaacaag ctggaagaga tcctttacgg cgtgctgcca caggtaatgc cgctgctgat
    20761 ctcctactcc ctctatcgct tcgaatccaa cgtccgctcg gcgaccgtcg tcggcatggt
    20821 cggtgcaggc gggatcggcg tcaccctgtg ggaagcgatt cgcggtttcc agttccaaca
    20881 aacctgcgcc ctgatggtgc ttatcatcgt cacggtcagc ctgctggatt tcctctctca
    20941 acggttgcgt aagcacttta tctgataagt gaggcattga tatctatgca cttgtctaca
    21001 catccgacca gctacccaac acgctatcag gagatagccg caaaacttga gcaggagctt
    21061 cgtcaacact atcgctgcgg cgactatctt cccgccgagc agcaactggc ggcgcgcttt
    21121 gaggtaaacc gccacaccct gcgccgcgcc atcgaccaac tggtggaaaa aggctgggta
    21181 cagcgccgtc agggcatcgg cgtgctggtg ctgatgcgcc cgttcgatta cccgctcaac
    21241 gcccaggcgc gttttagcca gaacctgctg gaccagggca gccatcccac cagcgaaaaa
    21301 ctgctgtcgg tattgcgccc cgcgtccggt cacgttgctg acgcgctggg gattaccgag
    21361 ggtgagaacg ttatccattt gcgcaccctg cgtcgcgtca acggcgtcgc gctctgttta
    21421 atcgaccact actttgcgga cctcaccctc tggccgacac tgcaacgttt cgacagcggt
    21481 tcgctgcacg attttctgcg cgagcaaacc ggaattgcgc tgcgccgcag ccagacgcgg
    21541 atcagcgccc gccgcgccca ggccaaagag tgccagcgcc ttgaaatccc gaatatgtcg
    21601 ccgctgctgt gcgtgcgcac ccttaaccac cgtgacggtg aaagcagccc ggcggagtac
    21661 tccgtcagcc tgacgcgcgc cgacatgatt gaattcacta tggagcactg aatgcacgca
    21721 gataccgcga cccgccagca ctggatgtcc gtactggcgc acagccaacc ggctgaactg
    21781 gcagcccgcc tgaacgcgct gaacatcacc gccgactatg aagtgatccg cactgccgaa
    21841 actggcctgg tgcagattca ggcgcgaatg ggcggtaccg gcgaacgctt ttttgccggc
    21901 gacgccacgc tgacccgtgc cgccgtacgc ctgactgacg gcaccctcgg ctacagctgg
    21961 gtgctggggc gtgataaaca gcacgccgaa cgctgcgcgc tgattgatgc gctgatgcag
    22021 caatctcgcc actttcaaaa cttatcagaa acccttattg ccccgctgga cgctgaccgt
    22081 atggcacgca ttgccgcacg ccaggccgaa gtgaacgcca gccgggtcga cttctttacg
    22141 atggttcgcg gagacaacgc atgaccctgg aaaccgcttt tatgcttccc gtgcaggatg
    22201 cccagcacag ttttcgtcgc ctgttaaagg ccatgagcga gccgggcgtg attgtcgccc
    22261 tgtatcagct caaacgcggc tggcagccgc tgaatatcgc caccaccagc gtgctgctga
    22321 cgctggccga taacgacacg ccggtgtggc ttgccacgcc attaagtaac gatatcgtca
    22381 atcagagcct gcgttttcat accaacgcgc cgctggtcag ccagccggaa caggcgacct
    22441 tcgcggtgac ggatgaggcg atttccagcg aacagctcaa cgccctttcc accgacaccg
    22501 ccgttgcgcc ggaagcgggc gcgacgctga ttttacaggt cgccagcctg agcggcggac
    22561 gcatgttgcg tctcaccggc gcgggcattg ccgaagaacg aatgatcgct ccgcagctgc
    22621 cagagtgcat tctgcacgaa ctcaccgagc gcccgcaccc gttcccgctc ggcatcgacc
    22681 taatcctgac ctgcggcgag cgcctgctgg ctatcccgcg aaccactcat gtggaggtgt
    22741 gctgatgtac gttgccgtga aagggggcga aaaggcgatc gacgccgccc acgccctgca
    22801 agagagccga cgccggggcg ataccgattt gcccgaactg agcgtcgccc agattgaaca
    22861 gcagcttaac ctcgcggtgg atcgcgtgat gacagaaggc ggcattgccg accgcgaact
    22921 ggcggcgctg gcgctgaaac aggccagcgg cgataacgtc gaagcgattt tcctgctacg
    22981 cgcctaccgc accacgctga cgaaactggc ggtaagcgag ccgctcgaca ccacggagat
    23041 gcgtctcgaa cggcgtattt cagcggttta taaagatatt cccggcggcc agctgcttgg
    23101 cccaacctac gactacaccc atcgcctgct cgatttcact ctgctggcaa acggcgaagc
    23161 gccgacgctg accaccgccg acagcgaaca acagccgtcg ccgcacgttt tcagcctgct
    23221 ggcgcgtcag gggctggcga agtttgaaga ggatagcgga gcacagccgg atgacatcac
    23281 ccgcacgccg ccggtttacc cctgttcacg ttcttcccgt ttgcagcagt taatgcgcgg
    23341 cgacgaaggc tatttgctgg cgctggctta ctccacccag cgcggttacg ggcgcaacca
    23401 cccgttcgcg ggcgagatcc gcagtggtta catcgacgtg tcgattgtgc cggaagagct
    23461 gggatttgcg gtaaacgtcg gcgaactgct gatgaccgag tgtgaaatgg tcaacggttt
    23521 tatcgacccg ccggatgagc cgccgcactt cacgcgcggc tacgggctgg tattcggcat
    23581 gagcgagcgc aaagcgatgg caatggcgct ggttgaccgt gcgttgcaag ccccggaata
    23641 cggcgagcac gcgacaggcc cggcgcagga tgaagagttc gtgctggcgc atgccgacaa
    23701 cgtcgaagcc gcaggctttg tctcgcacct caaactcccg cactacgtcg atttccaggc
    23761 cgaactggag ctactcaaac gtctgcaaca ggagcagaac catggctaat ctgagcggct
    23821 acaactttgc ctacctcgac gagcagacca aacgcatgat ccgccgcgcc atcttaaaag
    23881 cggtggcgat ccccggttat caggtaccgt ttggcgggcg cgagatgccg atgccgtacg
    23941 gctggggaac tggtggtatc cagctcaccg ccagcgtgat tggcgaaagc gacgtgttga
    24001 aggtgattga ccagggtgcg gatgacacca ccaacgccgt gtcgattcgc aacttcttta
    24061 agcgcgtgac cggggtaaac accactgaat gtacggacga tgcgacgctt atccagacgc
    24121 gtcaccgcat ccccgaaacg ccgctgaccg aagatcagat cattatcttc caggtgccaa
    24181 tcccggaacc gctgcgcttt atcgagccgc gcgaaacgga gacccgcact atgcacgcgc
    24241 tggaggagta cggcgtgatg caggtgaaac tgtatgaaga tatcgcccgc ttcggtcata
    24301 tcgccaccac ctatgcttat ccggtgaagg tgaacgggcg ctacgtgatg gacccgtcgc
    24361 cgatcccgaa attcgataac ccgaaaatgg acatgatgcc cgccctgcag ctgttcggcg
    24421 cagggcgcga gaagcgcatc tatgcggtgc cgccgtttac ccgcgtggaa agtctcgatt
    24481 tcgacgatca cccgttcacc gttcagcagt gggatgagcc atgcgccatc tgcggatcga
    24541 cccacagcta tcttgatgaa gtggtgctgg atgacgccgg aaaccgcatg tttgtctgct
    24601 ccgataccga ttattgccgc caacagagcg aggcgaaaag ccaatgaatc aaccgttact
    24661 ttcggtcaat aacctgaccc acctttacgc gccgggcaaa ggctttagcg atgtctcttt
    24721 tgatttatgg ccgggggaag tgctgggcat tgtcggggaa tccggctccg ggaagaccac
    24781 gctgctgaag tcgatctccg cgcgtctgac gccgcagcag ggggagattc actatgaaaa
    24841 ccgttccctc tatgcaatga gcgaggccga ccgccgtcgc ctgctgcgca ccgaatgggg
    24901 cgtggtgcat cagcatccgc tcgacggcct gcgccgccag gtgtcggcag gcggcaatat
    24961 cggcgagcgg ctgatggcga ccggggcgcg acattacggc gatattcgcg ctaccgcgca
    25021 gaagtggctg gaagaggtgg agattcccgc caaccgtatc gacgacctgc cgaccacctt
    25081 ttccggcggt atgcagcagc gtttgcagat tgcccgcaac ctggtgacgc atccgaagct
    25141 ggtgtttatg gatgaaccga ccggcgggct ggatgtgtcg gtgcaggccc gcctgctcga
    25201 cctgctgcgc ggactggtgg tggagctgaa cctcgcggta gtgattgtca cccacgattt
    25261 aggcgtcgcc cgcctgctgg cggaccgttt gctggtgatg aagcaggggc aagtggtgga
    25321 gagtgggtta accgaccgcg tgctcgacga cccgcatcat ccgtatacac agctgctggt
    25381 gtcatcggtt ttgcagaatt gagccggtgc cggatgcggc gtaaacgcct tatccggcct
    25441 acaaatgcgc tccccgtagg tcggataaga cgcgtcagcg tcgcatccga cacccgaacc
    25501 acgaggcgaa aaatgattaa cgtacaaaac gtcagtaaaa ccttcatcct gcaccagcaa
    25561 aacggcgtgc gcctgcccgt cctcaaccgc gcctcgctca tcgtcaacgc gggcgaatgc
    25621 gtggtgctcc acggccattc cggcagcgga aaatcaaccc tgctacgctc gctgtacgcc
    25681 aactatctgc ccgacgaagg gcaaatccag atcaaacacg gtgacgaatg ggtcgatctg
    25741 gtcaccgcgc cagcgcgcaa agtggtggag atccgcaaaa ccaccgttgg ctgggtgagc
    25801 cagtttctgc gcgtcatccc gcgtatttcg gcgctggaag tggtgatgca gccgctactc
    25861 gataccggcg taccgcgtga agcctgcgcc gctaaagccg cgcgtctgct cacccgcctg
    25921 aacgtgccgg aacgcctgtg gcatctggcg ccatcgacct tctccggcgg cgaacagcag
    25981 cgcgtcaaca tcgcccgcgg ctttatcgtc gactacccca ttctgctgct cgacgaacct
    26041 accgcctcgc tggacgccaa aaacagcgcc gccgtagtcg agctgattca cgaagccaaa
    26101 gcccgtggcg cggccatcgt cggcatcttc cacgacgaag ccgtacgtaa tgacgttgcc
    26161 gaccgcctgc acccaatgga agcctcttca tgattatcaa taacgtcaaa ttggtgctgg
    26221 aaaacgaagt ggtacacggt tcactggagg tgcaggacgg cgaaatccgc gcctttgccg
    26281 aaagccagag ccgcctgccg gaggcgatgg acggcgaagg cggctggctg ctgccggggc
    26341 tgattgagct gcataccgat aatctggata aattcttcac cccgcgcccg aaagttgact
    26401 ggcctgccca ctcggcgatg agcagccacg acgcgctgat ggtggcgagc ggcatcacca
    26461 ccgtactgga tgccgtggca attggcgacg tgcgcgacgg cggcgatcgg ctggtgaatc
    26521 tggagaagat gatcaacgcc atcgaagaga cgcagaaacg cggcgtcaac cgcgccgagc
    26581 accgtctgca tctgcgctgc gaactgccgc atcacaccac gctgccgctg tttgaaaaac
    26641 tggtgcagcg cgagccggtg acgctggtgt cgctgatgga ccactcgccg ggccagcgcc
    26701 agttcgccaa tcgcgagaag tatcgcgaat attatcaggg caaatactcc ctcactgatg
    26761 cgcagatgca gcagtacgaa gaagagcaac tggcgctcgc cgcacgctgg tcgcagccga
    26821 atcgcgaatc catcgccgcc ctgtgccgcg cgcgaaaaat tgcgcttgcc agccacgatg
    26881 acgccaccca cgcccacgtt gctgaatctc accagcttgg cagcgtgatc gccgaatttc
    26941 ccaccacgtt cgaagcggcg gaagcctcgc gcaagcatgg catgaacgtg ctgatgggcg
    27001 cgccgaatat tgtgcgcggc ggctcgcact ccggcaacgt ggcggccagt gaactggcgc
    27061 agcttggcct gctggatatc ctctcttccg actactaccc cgccagcctg ctcgatgcgg
    27121 catttcgcgt cgccgatgac gagagcaacc gctttacgct gccgcaggcg gtgaagctgg
    27181 tgactaaaaa tccagcgcag gcgcttaatc tccaggatcg cggggtgatt ggcgagggca
    27241 aacgcgcgga cttggtgctg gcgcatcgca agggcaatca cattcatatc gaccacgtct
    27301 ggcgtcaggg taaaagggtg ttctgatgat gggaaaactg atttggttaa tggggccgtc
    27361 cggctccggg aaagatagcc tgctggcgga actccgcctg cgggaacaaa ctcagctact
    27421 ggtggcgcat cgctacatca cgcgcgacgc cagcgccgga agcgaaaacc atatcgctct
    27481 gagcgagcag gagtttttta cccgcgcggg gcagaaccta ctggccttaa gctggcacgc
    27541 taacggtctg tattatggcg tcggcgtcga gatcgacctc tggctgcacg ccggattcga
    27601 cgtgctggtc aacggctcac gcgcccatct gccgcaggcg cgggcgcgct atcaatcggc
    27661 gctgctgccc gtctgtttac aggtttcgcc ggagatcctc cgccagcgcc tggaaaaccg
    27721 tggccgtgaa aacgccagtg aaattaacgc ccgcctggcg cgcgccgccc gctatactcc
    27781 acaggattgc catacgctca acaatgacgg cagcctgcgc cagtcggtcg acacgctgct
    27841 gacgctgatt catcagaagg agaaacacca tgcctgcttg tgagcttcgc ccggccacgc
    27901 agtatgatac cgacgcggtt tacgcgctga tttgtgagct aaaacaggcg gagtttgacc
    27961 accacgcatt tcgcgtgggc tttagcgcca atctgcgcga cccgaacatg cgctatcatc
    28021 tggcgctgct ggatggcgag gttgtcggca tgatcggcct gcatttgcag tttcatctgc
    28081 atcatgtcaa ctggatcggc gaaattcagg aactggtagt gatgccgcag gcgcgcggcc
    28141 tgaacgtcgg tagcaagttg ctggcgtggg cagaagaaga agcccgccag gccggggccg
    28201 aaatgaccga gctttcgacc aacgtgaagc gccacgacgc gcaccgtttc tatctgcgag
    28261 aaggctacga gcagagtcac ttccgcttca ccaaggcgct gtaacatgag tctgaccctc
    28321 acgctcaccg gcaccggcgg cgcacagggc gttcc
//
LOCUS       DAJTXQ010000044        28254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000044 DAJTXQ010000000
VERSION     DAJTXQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.044"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO515_001829"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO515_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6575775.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO515_001830"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO515_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6575776.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO515_001831"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO515_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6575777.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO515_001832"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO515_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6575778.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO515_001833"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO515_001833"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6575779.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO515_001834"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO515_001834"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6575780.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO515_001835"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO515_001835"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6575781.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OO515_001836"
     CDS             complement(4604..5326)
                     /locus_tag="OO515_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6575782.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO515_001837"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO515_001837"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6575783.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OO515_001838"
     CDS             6526..6744
                     /locus_tag="OO515_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6575784.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO515_001839"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO515_001839"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6575785.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OO515_001840"
     CDS             complement(7478..7678)
                     /locus_tag="OO515_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6575786.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO515_001841"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO515_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6575787.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO515_001842"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO515_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6575788.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO515_001843"
     CDS             10175..12088
                     /locus_tag="OO515_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6575789.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO515_001844"
     CDS             12088..13110
                     /locus_tag="OO515_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6575790.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO515_001845"
     CDS             13104..13322
                     /locus_tag="OO515_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6575791.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO515_001846"
     CDS             13376..14245
                     /locus_tag="OO515_001846"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6575792.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OO515_001847"
     CDS             complement(14300..14704)
                     /locus_tag="OO515_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6575793.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OO515_001848"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OO515_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6575794.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OO515_001849"
     CDS             15689..17779
                     /locus_tag="OO515_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6575795.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO515_001850"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO515_001850"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6575796.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO515_001851"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO515_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6575797.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OO515_001852"
     CDS             complement(19747..20349)
                     /locus_tag="OO515_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6575798.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OO515_001853"
     CDS             complement(20339..20506)
                     /locus_tag="OO515_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6575799.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO515_001854"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO515_001854"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6575800.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OO515_001855"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OO515_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6575801.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OO515_001856"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OO515_001856"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6575802.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OO515_001857"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OO515_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6575803.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OO515_001858"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OO515_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6575804.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OO515_001859"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OO515_001859"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6575805.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6839 a         7591 c         7128 g         6696 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctg
//
LOCUS       DAJTXQ010000045        27876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000045 DAJTXQ010000000
VERSION     DAJTXQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.045"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..450
                     /gene="tnpA"
                     /locus_tag="OO515_001860"
                     /pseudo
     CDS             19..450
                     /gene="tnpA"
                     /locus_tag="OO515_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(581..1904)
                     /locus_tag="OO515_001861"
                     /pseudo
     CDS             complement(581..1904)
                     /locus_tag="OO515_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1904..3520)
                     /locus_tag="OO515_001862"
     CDS             complement(1904..3520)
                     /locus_tag="OO515_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6575806.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3816..4532
                     /locus_tag="OO515_001863"
     CDS             3816..4532
                     /locus_tag="OO515_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6575807.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4529..6190)
                     /locus_tag="OO515_001864"
     CDS             complement(4529..6190)
                     /locus_tag="OO515_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6575808.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6386..6814)
                     /gene="ibpB"
                     /locus_tag="OO515_001865"
     CDS             complement(6386..6814)
                     /gene="ibpB"
                     /locus_tag="OO515_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6575809.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6926..7339)
                     /gene="ibpA"
                     /locus_tag="OO515_001866"
     CDS             complement(6926..7339)
                     /gene="ibpA"
                     /locus_tag="OO515_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6575810.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7333..7405)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7645..7977
                     /locus_tag="OO515_001867"
     CDS             7645..7977
                     /locus_tag="OO515_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6575811.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7979..9193)
                     /locus_tag="OO515_001868"
     CDS             complement(7979..9193)
                     /locus_tag="OO515_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6575812.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9294..10358
                     /gene="cbrA"
                     /locus_tag="OO515_001869"
     CDS             9294..10358
                     /gene="cbrA"
                     /locus_tag="OO515_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6575813.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10355..11692)
                     /locus_tag="OO515_001870"
     CDS             complement(10355..11692)
                     /locus_tag="OO515_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6575814.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11767..12915)
                     /gene="dgoD"
                     /locus_tag="OO515_001871"
                     /pseudo
     CDS             complement(11767..12915)
                     /gene="dgoD"
                     /locus_tag="OO515_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12912..13529)
                     /gene="dgoA"
                     /locus_tag="OO515_001872"
     CDS             complement(12912..13529)
                     /gene="dgoA"
                     /locus_tag="OO515_001872"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6575815.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13513..14391)
                     /gene="dgoK"
                     /locus_tag="OO515_001873"
     CDS             complement(13513..14391)
                     /gene="dgoK"
                     /locus_tag="OO515_001873"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6575816.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14388..15077)
                     /gene="dgoR"
                     /locus_tag="OO515_001874"
     CDS             complement(14388..15077)
                     /gene="dgoR"
                     /locus_tag="OO515_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6575817.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15046..15678
                     /locus_tag="OO515_001875"
                     /pseudo
     CDS             15046..15678
                     /locus_tag="OO515_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15724..16536)
                     /gene="yidA"
                     /locus_tag="OO515_001876"
     CDS             complement(15724..16536)
                     /gene="yidA"
                     /locus_tag="OO515_001876"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6575818.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16651..17049)
                     /locus_tag="OO515_001877"
     CDS             complement(16651..17049)
                     /locus_tag="OO515_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6575819.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17289..19703)
                     /gene="gyrB"
                     /locus_tag="OO515_001878"
     CDS             complement(17289..19703)
                     /gene="gyrB"
                     /locus_tag="OO515_001878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6575820.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19732..20805)
                     /gene="recF"
                     /locus_tag="OO515_001879"
     CDS             complement(19732..20805)
                     /gene="recF"
                     /locus_tag="OO515_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6575821.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20805..21905)
                     /gene="dnaN"
                     /locus_tag="OO515_001880"
     CDS             complement(20805..21905)
                     /gene="dnaN"
                     /locus_tag="OO515_001880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6575822.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21910..23313)
                     /gene="dnaA"
                     /locus_tag="OO515_001881"
     CDS             complement(21910..23313)
                     /gene="dnaA"
                     /locus_tag="OO515_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6575823.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23610..23690
                     /locus_tag="OO515_001882"
     CDS             23610..23690
                     /locus_tag="OO515_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575824.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23920..24060
                     /gene="rpmH"
                     /locus_tag="OO515_001883"
     CDS             23920..24060
                     /gene="rpmH"
                     /locus_tag="OO515_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6575825.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24077..24436
                     /gene="rnpA"
                     /locus_tag="OO515_001884"
     CDS             24077..24436
                     /gene="rnpA"
                     /locus_tag="OO515_001884"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6575826.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24400..24657
                     /gene="yidD"
                     /locus_tag="OO515_001885"
     CDS             24400..24657
                     /gene="yidD"
                     /locus_tag="OO515_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6575827.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24660..26306
                     /gene="yidC"
                     /locus_tag="OO515_001886"
     CDS             24660..26306
                     /gene="yidC"
                     /locus_tag="OO515_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6575828.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26412..27776
                     /gene="mnmE"
                     /locus_tag="OO515_001887"
     CDS             26412..27776
                     /gene="mnmE"
                     /locus_tag="OO515_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6575829.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6703 a         7541 c         7116 g         6516 t
ORIGIN      
        1 agtcgtttca caggccaaat gatactttgg tctgtgaaaa aagaaaccga tatccggcgt
       61 ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa ataccgacgt
      121 cagatttttg actatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct
      181 gattttgaag ctgaactggt ttaaatggat ggcgaaccag atcacgtcca tttgttaatt
      241 aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt
      301 aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa aggcgttttg
      361 tggagtcctg gctattttgc caatagctgc ggaggtgcgc caatatccgt catccgccaa
      421 tacattgaac aacagcaaac acctggttag gtggaaaacc gcgccttata tccccgccct
      481 gaaggacggg gttttacggc gcaccggata aatttggtta tgcgccgtca gccgtgctaa
      541 agaatacgga ccagcaccca acggatgcca gtccacacaa ttaacgtaac tctggccagt
      601 acgctttgtt ggcctccagc aactcatcca gaatagcttt ggcgaccgag gcgctcggta
      661 ccgttttcga taacgtaatc gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt
      721 ccacgaccag tttttccacc gccacttgct gactcattaa ccctttctga aactgcggga
      781 tatcgccgac caccagtggt tctggtccgt tgtgcccgac cagacacggg atctcgacca
      841 tcgcttcatc atcaaagtta tggattgccc cgttgttagg cacaatcagc aacatccgct
      901 cctgagtgtt gaaggcaatt gccgccgcca gatcgacgat gtatgacgca tgttcgtcaa
      961 tctccagctt ggccgccgct gaatttcctg ccgctgtaat ggcgcggcaa gcatcgaaaa
     1021 cctgtttctc gcgatgttcc attcacctca ttcgcgcggg tatgttcagg gttggaatgc
     1081 tgaacaacat aatccgggaa gagataatat ttcagatagg tgttcggcaa tgtatccggg
     1141 tccagtgcct ggacatcccg cgcttttgca taggtgtcgt tccagctcgc ttcagtatgt
     1201 gtatcttgct gcggtggaac ataaccgtgc ttcgagacat attgccgcag ctggggcatc
     1261 aggtcgttgc cctgcaaatc ttcaattgat gtccaccagc caaagtggtt caggccgtag
     1321 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
     1381 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgtg
     1441 gcttctgcga caatggctgc cgggttggag tagttgagca tccaggcatt tggtgaatat
     1501 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
     1561 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
     1621 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
     1681 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ctctttaagg
     1741 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
     1801 agtgcacgaa gcgggaaacg ctcctgattc gccaggagca tcaacacgat gcctggcgta
     1861 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
     1921 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcagct
     1981 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
     2041 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatgc
     2101 gtagacgcgt cgcacaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
     2161 ggataccggc agcctgatcc agctcttttt ttggctctgc cgcggtggtt tggcctcgcg
     2221 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
     2281 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
     2341 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
     2401 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
     2461 acatcacggt cgacattgag gccgcaagca cagcgtgtac cgcaaacagc aacggtgaaa
     2521 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
     2581 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
     2641 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
     2701 ccgggaacaa cgatttcagc ggctcggcgc tcaaactgaa ctcttgcaga tgctgcgccc
     2761 agtacatctg aatgccgcct tcaacagctg ccggacccaa gataaacggt ccgtagatga
     2821 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cagaccccaa
     2881 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccgtttgt acttttggcc
     2941 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
     3001 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
     3061 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
     3121 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
     3181 cgaagtagct tccccaggtc attcccatcg cgttgatgaa atagttccag gtcaggaaac
     3241 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
     3301 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
     3361 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
     3421 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
     3481 taaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
     3541 attctgtaag taggaagcct gattggttat gcggcaggcc tggagcaagg ataggaagat
     3601 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
     3661 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
     3721 cgaacacatc tttaaaaaaa agatgttttt tcaatcgatt aagcagaact tgtgtgcgca
     3781 ttacccgggc ttgcaggcaa aaaagagatc tagagatgat ctacaaaagc attgcggagc
     3841 ggttaagaat tcgacttaac tccgcagatt tcacgctaaa cagccttctt ccgggtgaaa
     3901 aaaagctggc ggaagagttt gcggtatcgc ggatgaccat ccgtaaagcc attgacctgc
     3961 tggtagcgtg ggggctggtg gtccgccgcc acggtagtgg cacttacctg gtgcgcaaag
     4021 atgtactgca tcaaaccgcc agtctgaccg ggctggtgga ggtgttaaaa cggcagggaa
     4081 aaactgtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg gccattgcca
     4141 gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg
     4201 aagggaaacc gctgatgctg gaagacagct atatgccggt aaaactgttc cgtaatcttt
     4261 cgctgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc gggattttga
     4321 ttggcggtaa ttatgaaagc ctgacgccgg tgctcgccga tagactgctg gcgcggcaaa
     4381 tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat agcgagagcg
     4441 gggagttttt gaattattca gtgatgttca gaaatgccag cgaataccag gtggagtacc
     4501 atttacggcg actccacccg gaaaagagtt aaccgatact ccagaagagc accgccagta
     4561 attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg taagagagcg
     4621 ccgccgcacc gctagttgga tgaagattat tagcaaacgc cagcgccgga ggatcggtca
     4681 tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac atccgcgcca
     4741 gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca atccagctta
     4801 gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga ccaacgaccg
     4861 agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc ggcataaacc
     4921 agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc agcagaccgc
     4981 ccgccagccc cagtttcaac gcggccggga atcccggcac aaagacggga atagaaccta
     5041 acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt tgcagttttt
     5101 gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc cccaccaggt
     5161 tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg accccggcac
     5221 ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt
     5281 ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca gtgtctttcg
     5341 tggacagcga agtatcgacc tcctgaccaa tcaccagttg cgcattatgt aaatccgctg
     5401 gctgacccac cagatgcagc aaatcgccca gttggataat ggtatctggc gaaggaactt
     5461 ttagggtttc ttcgcgtttc agacgcgagc agataatttt gtcgccgttg agaatcggta
     5521 catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg atagtcttga
     5581 tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc tcgacattga
     5641 cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg ccgaatggat
     5701 acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta cccaggtcgc
     5761 gcaaaatctg ctgccctgcc cccagcgctg gcgtattggt aaccgcaccg gagaaaatcc
     5821 ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta accagaccac
     5881 cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg
     5941 caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc aggccaaatt
     6001 cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa acaaaatggc
     6061 cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg cgaaatttga
     6121 cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc gttaatgcta
     6181 tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat tatggctgaa
     6241 tcggtgaagc agatttgcct tatagcgcac attattacgg gaaagaacgt gccgaaatat
     6301 cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag ccccaccaga
     6361 atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg ctgatagcga
     6421 tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc agtaaaccgt
     6481 ttacgaaagt tgcgccagag acttccatat tttcagccag cgtaaagctc aggctaaatg
     6541 gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc tccggcgtgc
     6601 cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga cggaaacctg
     6661 ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg tacggcggga
     6721 agctctgact ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt
     6781 gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct aagaagcgag
     6841 taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat ggagatgtca
     6901 ggccgcgcca ggcggcctta aggaattagt tgatttcgat acggcgcggt tttttcgctt
     6961 ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta gcgccacgaa
     7021 catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg atgccctggt
     7081 acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc agcagattat
     7141 cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata gcaatgcggt
     7201 aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta ctctggctct
     7261 ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg taaagcgggg
     7321 ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt aagaccttcc
     7381 acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg actacgatgt
     7441 aaaaatgggt ctggaaatga acttttcaag ccttatcagc ggactttttt ttgcagttta
     7501 tggtctattg catagactga gggggcagca cgaaacgtta aagtgtgata gccgccacag
     7561 agcgacgtaa tgatgatgtt attttcagca agggatcgct ataactcatc aggccaacac
     7621 ccacgatcaa cagatgaatt tattatgata agaaatgtgt tgttagcgtt catgatatgc
     7681 agcggaatgg cattactcgg aggatgctcc agcgtgatgt cccataccgg agggaaagaa
     7741 ggaacgtatc ccggcacccg cgccagcgcc accatgattg gcgatgatga aaccaactgg
     7801 ggcactaagt cgctggcgat tctcgatatg ccgtttaccg ctgtgatgga cacgctttta
     7861 ttaccgtggg atgtgttccg caaagacagc tcggtgcgct cacgcgttga aaaaagcgaa
     7921 gccaacgcgc aagccaccaa tgcggttatt ccaccggcca gaatgcctga taactgattt
     7981 agcgatcagt ttcggtgatc cacagctggc caccttccat ccacgccagc cattgaccat
     8041 ccggcgagaa gaccacggcg tccgcagaag gtggattggc gtggttttcg gttaaatact
     8101 caacctcgcc gctttgcgca tgggcacaag caattcgatt atccagcaca aagcccaacc
     8161 attctcctga cggatgccag ttaaatgcag actgaatatc cgttttgtta tgggttaact
     8221 ggcgcggctc gccgccctgt ggcgagataa gccacagttg cacaatgccg ttatcatcac
     8281 gcattaaaaa cgcgatttgc gtaccctgcg gattacagcg cacccagtgg cgcgggacgt
     8341 tgactaaccc cggataagcc cgatggtggg taaaggttaa acgtcgctgc acgacgccac
     8401 gcggtggcgc gggcagggtg gtttccgttc cacttaacgg cgcatcacct gccgctttcc
     8461 agccagcttc atcttgcggc aactcaacga taaacagctc cggcactttc tcaccctttg
     8521 gcgaaagtgt gtcgccaata aacgccagcg cgtgatttcc tacccatcct tcttcataag
     8581 cacgattgat ttcatcactg ccaggctgcg gcgtgggcgt ggttttactc accagcacac
     8641 accagtggct gccgctgtat tcacgcggat gctgcttttg tacgttgacc ggaccaaacg
     8701 gcgcagcaac accgacgttt cgcaaatcca gcgccggatc gagttcatgc attacatggt
     8761 cgttataggt aaagctcacc ctttcaccgt tcgggctaaa gacatgcaca tggctgccgc
     8821 cgcgcagcgc tcctggcgta tacggcgcgg taatatccat tgcatcgaga ttgctcacct
     8881 tgccgccttc agcaatgact ccgcgccgat gatggaagtc gtaatgccat gtttcatcag
     8941 gattttccgg gccgtgaata aagacatatt tctctgactt cggatgaacg gtcaccacgc
     9001 cgacgtgtgc gccctgtgac gcacagtaga taacctcgac ctcaccggta tggatattca
     9061 cacgctcaat ggtctcgccg gtaaacgacg ccccagaagg acgcacgtca aataccagcc
     9121 actggctgtc gggcgtccag gtattggtat tggtgagcag gtgattacgg ggagcaaagg
     9181 ttatctgttt catggcgcgg tcctgataca aaacgggtcc agccatcata cttcacaagg
     9241 acttcacttt cagacaatag tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac
     9301 atttcgacgt ggcgattatt ggcctcggcc cggcagggtc ggcgttggca cgaaagttag
     9361 ccggcaaaat gcaggtgatc gcgctggata aaaagcacca gcatggtact gaaggtttca
     9421 gcaaaccttg tggcggtctg ctggcaccgg acgcgcagcg atcttttatt cgcgatgggc
     9481 tgacgcttcc tgtcgatgtg atcgctaatc cgcagatttt cagcgtcaaa accgtcgacg
     9541 tcgccgcatc gctcacgcgt aactaccagc gaagctatat caatattaat cgccatgctt
     9601 tcgacttgtg gatgaaatcg ctgatccccg ccagcgttga ggtttatcac gacagcctgt
     9661 gtcgaaaaat ctggcgtgag gatgataaat ggcatgtcat ttttcgtgca gacggctggg
     9721 agcagcatat taccgcccgc tatctggtcg gtgccgatgg tgccaactcg atggtgcggc
     9781 gacatctcta cccggatcat caaatccgta aatatgtcgc aatccagcag tggttcgcag
     9841 agaaacatcc ggtgccgttc tactcctgca tctttgataa tgcgataact gactgttatt
     9901 catggagtat cagcaaagac ggttatttta tctttggcgg tgcctatcca atgaaagacg
     9961 gtcagacgcg tttcacgact ctgaaagaga aaatgagcgc ctttcagttc cagtttggca
    10021 aagcggtgaa aagcgaaaaa tgcacggtgc tgtttccctc acgctggcag gattttgtct
    10081 gcggtaagga caacgccttt ctgattggtg aagcggcggg atttatcagc gccagctcgc
    10141 tggaggggat tagctatgcg ctggatagcg cagacattct gcgttcggtg ttactgaagc
    10201 agccagagaa gctcaatacc gcttactggc gcgccacccg caaactgcgt ttaaaactct
    10261 tcggcaagat agtaaaaagc cgatgcctga ccgcaccggc tttaagaaag tggattatgc
    10321 gcagtggtgt ggcacatatt ccacagttga aggattagcc aacgcgcttc acatcgccca
    10381 ccagcaggat ataagagagc gcgccaatca acgcgacggc ggagatataa accagtgcag
    10441 gtgcgaaacc gtaaccctgc gccaggtacc ccaccaccag cggaacggtg atgccgccca
    10501 gaccaccggc gaagttaaac acgccgccgg ttaaaccaat caggcgcatc ggtgccagag
    10561 aagaaaccag cgaccaggta attgaagcaa aaccgttacc gaagaatgcc agcgccatca
    10621 ggcacataat catcatcgga tcgttagtgt agttagcgcc cataatgcag gtggagatca
    10681 gcaagccgca gataatcggc gttttacgcg caaagcccag tgaaaagccc ttacgtacca
    10741 gcagatccgc tacccagcca gagagcagga cgccgacaaa cgccgcgagg aatggcaccg
    10801 tggtcataaa gcctgctttc agcgccgtga ttcctttttc ctgggttaaa tagttcggga
    10861 accaggttaa gaaaaaccac agtgtagaag ccaccgcaaa ttgcccaaga tagacgccga
    10921 tcagtttacg atggaacacc agtttccagt ctttggctgt taacggctga cgtgcctctt
    10981 tcttcactgg cgcatcacca tccaccagac cgccgccatc acgaatgtaa tccagttcag
    11041 ctttgctgat gcctttggtc aggcgcggcg gctgataaac cttaaaccaa atcagcgacc
    11101 agataatgcc gataccacca gtcacaatga acacccagtg ccagctcaac atctcctgaa
    11161 tccagatcag cagcggagtc aaaaacgcca gaccgacaaa ctgaccagac gtataaaaac
    11221 caacggcgga ggcgcgttca tgttccggga accagctggt caccatccgg ttattggtcg
    11281 ggaaagcggg cgcttcgaaa ataccggtta tcgcacgcag accaattaat gacattaatc
    11341 ccgtggcaaa gccctggaat aaagtcgcca ctgaccagcc aaatatcgca ataaaataag
    11401 tcacgcgaga acctacgcga tctaaaaacc aaccgccggg gatctggcat agcgtataaa
    11461 gccaggcgaa ggccgaaaat acatagccca tttccgcttt cgtaatgccg aactcttcct
    11521 gaatatgggc ggaagccacg gccaggttag cgcggtcaac ataacaaatg accaccgtaa
    11581 taaagatcat caccagcgtc agataacgcc gacgccccgg ctttgctgca ttaacgggaa
    11641 tatccatagc gagctttctc cagattttgg gcatagcgaa gccgctcacc atgccctgta
    11701 atttacagag ggttatattt ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg
    11761 caggaattac cactctgcta cgctgttatc ttcatgacgc cagagtggat tacgccagtc
    11821 cggggcattt ttactgaact caataacttt agcttcgtcg atttccacgc ctaagcccgg
    11881 tttcgttagc ggtttaaaga agccgccaac catgctgaag tcttctttgt ttttcacaaa
    11941 gtcgagtaac tccgcgcctt tgttgtaatg aattcccata ctttgttcct gaagtaccgc
    12001 gttataggaa acaaagtcga tatgcagaca agccgccagt gcaatcggtc cgagcggaca
    12061 gtgcggcgca agggtcacgt cataggcttc tgccattccg gcaattttgt agcattcggt
    12121 aataccgcct gcatgggaga gatccggttg tagaatcgaa ataccgcctg cctccagcac
    12181 gcgtttaaaa tcgaaacgtg agaacatgcg ctcacccgcc gccagtggaa tatgcgtttg
    12241 tgccgccaat ttcgggtagt attcggcttg ttccgccagc accggctctt caataaacag
    12301 cgggcgatac ggctccagtt ctttaatcag cactttcgcc attggcgcgc tgacgcgacc
    12361 gtggaaatca agaccaaact caatctgatt gccaaaagct tcacgaattt gtgccacggt
    12421 gttgactgcc gcatctaccg cgcgggagtt atcaattagc cccagttctt cacaaccgtt
    12481 cagtttgaag gtatcgaagc cgatttcgcg cagggtttta ataccgtcga tcacatccgc
    12541 cggacgatcg ccgccgaccc aactgtaggc tttaattttg tcgcgaacca ggccgcccat
    12601 cagttgccag accgacgcat tcagcacttt acctttgata tcccataacg cctggtcgat
    12661 cccggcaatg gcgctcatca ggattggacc gccacgataa aatccggcgc gatacatcac
    12721 ttgccataag tcattgatgc gctatggatc ctgaccaatc aaatagtcac ccagctcgtg
    12781 aactgccgct tccaccgtgc gggcgcggcc ttcaatcact ggctcgcccc agccgacgac
    12841 gccttcatcg gtttcaattt tcaggaacat ccagcgggga ggtaaacgat acgtggtaat
    12901 tttggtgatt ttcattgcac tgcctctcga tacgccttaa caaatgctgc tgcctgctgc
    12961 gcggtgcgtt ctacggactg cccggcgcga tagagatcgc tgcctaagcc cgccccagca
    13021 caacctgcgt ctatccactg cgccaggttt tctggcgtca cgccgccaac ggcaaagact
    13081 gcgatgtccg atggcaatac cgcttttaac gctttgatgt attgcggacc aaaagccgat
    13141 gacggaaata ttttcagcgc ctgcgcgccc gcttcgagcg cggtaaaggc ttcggtcgcc
    13201 gtcgcgcagc cggggcagac ggtcatgccg tagcccaccg cacggcggat cacttcacta
    13261 tggatattgg gcgtaacgat gagctgacag cccatcctgg cgagcgcatc gacctgttca
    13321 ggtttcagta ccgtacctgc gccaatcaac gccttgtcgc catacgcatc aacgatggcg
    13381 ggaatgcttt gctcccattg tggggaattc agcgggattt caaccgcgtc gaacccggcg
    13441 tcaatcaccg cgccaacatg cgccagcgcc tcgtcgggct taatgccgcg caaaatggcg
    13501 atcagcggaa gtttagtttg ccactgcatg agcgatgctc cttataccag cctgaaatgc
    13561 cgtgtcgccc gccaccgccg tcacgtcgca acccatcgcc tgaaaggctt gctggtagcg
    13621 cgcggtcaac gatgttccgg cgacaagggt gatggcgtgt tgatgggcca catagtcgcg
    13681 catactggcg acctctgcgc caatcaacaa accagagaga aattcactga cctgttcacg
    13741 gggaagtgtt cccagcacat gcgaggcacg aacttcaaaa agctgcggca atatggcggg
    13801 cgtattaaga ccacgctcaa ggccagccgt gaaggcatcg gcagagtttt cctgcggcgg
    13861 caaacctgca ccaatcaatg agtgatttaa cagtaaatga tgtaattcac cggtcatcac
    13921 ggtgcgaaaa tcgttgattt gctggctatc ggcctgcacc catttgcaat gcgtaccggg
    13981 catgacataa agagaggaag gagccagagc tcgcgcgccg atcaattgag tttcttcgcc
    14041 gcgcatcaca ttgtggttat catcatgaga gacgcataat ccgggaataa tccagatatt
    14101 gtcgccaaca gacgttaatt gttcgccaat agacgaaaaa tgggcaggaa cagataaata
    14161 cggagcaact ttccagccga cattgctgcc gaccattcct gccattacca ctggcgtatt
    14221 ctcttcacgc cagtcggtcg tgacttctgc taacaccgca gccggagatt ttccgttcag
    14281 gcgcgtgacg cctgcttctg attgcctgct ctccaggcag tggtcgccct gataaagcca
    14341 ggcgcgcaga ttggtcgatc cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc
    14401 tttaaccttc gtgtcgagct ggcgatcatg gtaagcgccg cctgctctgc cgcatcgccg
    14461 tcctgatgcc gtatcgcatc gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg
    14521 gcctcatcgc ccatccaggt tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg
    14581 ctaagttgct gtaacaccgg gttatgcacc gactgcagca ccgcctcgtg gtagcgaata
    14641 tccgcttcgt taaacgcttc gcggtcctga ttgttggcaa tcatctcgtt cagcgccgat
    14701 tcaatctgcg ccagatcgct ggaggtcgcg cgctctgctg cccaacgggc aatcgccggt
    14761 tccaccagat ttcgcacttc gctcatggca ctgataagcc gtgggtcgta gtcgttttcc
    14821 agcacccatt gcagtacgtc agtgtcgagg taattccact ggttacgcgg tgccacaaac
    14881 gcgccgcgat aacgtttcat ttcaatcagc cgtttcgcca tcaacgaacg gaacacctca
    14941 cggatgatgt tgcgcgaggt tgcaaactcc tcacagagtt ccgcctcagc cggaagcggc
    15001 gagcctggta cgtatttgcc gtgaacgatc tgtttaccca gcgtaatgac gatgcgatcg
    15061 gttttattga gagtcatgga gagtccttgt gctctgtatg ttcttctcta ctttaccccg
    15121 atcgatgcat aatcaggaaa gtatcaatcg cagtcagaca ccatcacggg gctatcgcag
    15181 gcaaaggata gtaatggaac aaaaggctat gtttttgtag gggaatcgct ggattacctt
    15241 atcacagatg gtgccgatga catcgttaag atgctcaagg atccagctct taaccggcac
    15301 aatattcagg ttgctgatga cgcaagattt gttttaaatg cggggaaaaa gaaatttacc
    15361 ggcacaatat cactctatta caactggagc acagaagaag aaaaagcacg agtgacacaa
    15421 tatcgttttg cttgtggtgt tcagcactgt acccggacag tagaaaacct ggaaggtact
    15481 atccatgaga aaaataaaaa catggattac tcaaacgtaa tggctttcta ccatccattt
    15541 aaagtgcgat tttatgaata ccattcaccc agaggcattc cggatggtgt ttccgcagca
    15601 ttactgccag tgactgttac gctggacatc attactgcac cgctgcaatt tctggttgta
    15661 tatgcagtaa accaataatc agtaagcggg caaacgcgtt tatgctgttt gcccgcccac
    15721 agattaattc agcacatact tctcaatagc aaacgccacg ccatcttcaa ggttagattt
    15781 ggtgacaaag ttcgccactt ctttcaccga aggaatagcg ttatccatcg ccacaccgac
    15841 gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg ccaatcgcca tgatttcttc
    15901 cggtttaata cctaacacgt cggccagtga tttcacaccc gtacctttgt taacgcgttt
    15961 atcgaggatt tcgaggaagt acggcgcact tttcagcacg gtatatttct ctttcacttc
    16021 ctgcggaata cgcgcgatag cctggtcgag gatggcgggt tcatcaatca tcatcacttt
    16081 caggaactgg gtattggggt ccattttctc cgcttcgcag aacaccagcg gaatagtggc
    16141 aacgaaagat tcatgcactg tgtagtagct gatatcgcgg ttggcggtgt acagcgtggt
    16201 gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag agtttttcca ggaaacgata
    16261 gtcgtcatag ctgagagcag tttgcgccac ggtgctacca tcagctgcct tctgtactag
    16321 cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc atatgcagct ctttcaggta
    16381 gttgtgcaca cctgcatacg ggcgacccgt cgttagcacg acattcacgc cacgggcgcg
    16441 agctgcggca atcgcatttt taacggcggg tgaaatggtg tgatcgggca gcagaagggt
    16501 gccatccata tcgatagcaa tgagtttgat agccatgagt tccccaggta gattggttcc
    16561 tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa cacccgaggg aaaaggggga
    16621 tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag agtttccctt tcagcagttc
    16681 catacctgcg gaaagcagat cgttattggc ttgtggtgac acttcacctt gcggtgagag
    16741 cgcatcaata atcttcggca attgctctgc cagtaaactg gaagctgtac tggtatccac
    16801 gccaagtttt tgcccgagat cggacaccgc atttgtgccg agcgccgatt ccagttgctc
    16861 gccactaacc gattgattgc cctgttgatt actcagccag gttgagagaa tggcccctaa
    16921 gccgccactt tgcagttttt ccagcagcac ctgaatgccg ccctgctcct caacccaact
    16981 taaaatagcc tgatatttcc ccgcatcgcc tttcagaaag gcaccgacaa cttcatcaaa
    17041 aagccccatg ataatcacct gtaaagcgtt acgtgttgac ccaaaaagta tagatttgcg
    17101 ggatgataat tgcggattgc agaaataaaa agggcggaga tgatctccgc ccttttctta
    17161 tagcttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatca tgaaaattca
    17221 atacattgca agattttcgt aggcctgata agcgtagcgc atcaggcacg ctcgcatggt
    17281 tagcgccatt aaatatcgat attcgccgct ttcagggcgt tctcttcaat aaacgcacgg
    17341 cgcggttcaa cggcgtcgcc catcagcgtg gtgaacaact ggtcggcagc aatcgcatct
    17401 ttaacggtaa cgcgcagcat acgacggctt tccgggtcca tggtggtttc ccacagctgt
    17461 tccgggttca tctcgcccag acctttataa cgctggatgg agaggccgcg acgggactct
    17521 ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg gctgacgacg ctcgccacgt
    17581 tcgataaacg catcttcttc cagcaagcca cgcagtttct cacccagcgt gcagatacga
    17641 cgatattcgc caccggtgat aaactcgtga tccagcggat agtcagtatc cacaccgtgg
    17701 gtacgcacgc gaacaatcgg ttcaaacagg ttttgttcgg cattggtgtg gacatcaaac
    17761 ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc gttcacccag
    17821 cgggtaacgg tctgctcatc agagaggtcg gcttccgtca gcgtcggctg atagataagc
    17881 tctttcagca ttgctttcgg ataacgacgc tccatgcggt tgatcatttt ctgcgtcgcg
    17941 ttgtactcag acaccaattt ctctaacgct tcgccagcca atgccggtgc gctggcgttg
    18001 gtgtgcagcg ttgcgccatc cagcgcgata gagatctggt actgatccat cgcttcgtcg
    18061 tctttaatgt actgttcttg cttgcctttc ttcactttgt acagcggcgg ctgagcgatg
    18121 tagacgtgac cgcgttcaac gatttccggc atctgacgat agaaaaaggt caacagcagc
    18181 gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga tgatgatgct gtgataacgc
    18241 agtttgtccg ggttgtactc gtcacgaccg ataccacagc caagcgcggt gataagcgtc
    18301 gccacttcct gagaagagag catcttatcg aagcgcgctt tctcaacgtt gaggatttta
    18361 cccttcagcg gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc agagccgccc
    18421 gcggagtccc cttccaccag gtacagttcg gaaagcgccg gatcgcgttc ctggcagtct
    18481 gccagtttgc ccggcaggcc cgctaagtcg agcgcacctt tacggcgggt catttcacgc
    18541 gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa ttttgccaac cacgattttc
    18601 gcgtcggttg ggttttccag caggtattct gccagcagtt cgttcatctg ctgttcaacc
    18661 gctgatttca cctcagaaga aaccagtttg tctttggtct gggaggagaa tttcgggtcc
    18721 ggcactttca cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc ggtggcgcta
    18781 actttggctt ttttgctgta gccttctttg tccatgtagg cgttcagggt acgggtcatc
    18841 gccgcacgga agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt gttggtaaag
    18901 cagtagatgt tttcctggaa gccatcgttc cactgcaacg ccacttcgac gccaataccg
    18961 tctttttcag tggagaagta gaagatattc gggtggatcg gcgttttgtt cttgttcaga
    19021 tattcaacga acgccttgat gccgccttca tagtggaagt ggtcttcttt gccgtcgcgc
    19081 ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg acaactcacg cagacgtttc
    19141 gccagaattt catattcgaa ctcggtcaca ttggtgaagg tttcgaggct gggccagaaa
    19201 cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg ccaacggtgc ctgcggtaca
    19261 ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct ggataaccag ctccagtttt
    19321 tgcgacaggg cgtttactac cgaaacacca acgccgtgca gaccgccgga cactttatag
    19381 gagttatcgt caaatttacc gcctgcgtgc agaacggtca tgatcacttc cgccgccgat
    19441 acgccctctt ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg tacagagaca
    19501 gagttatcgg cgtgaatggt gacgataatt tctttacagt gacccgcgag cgcttcgtcg
    19561 atagcgttat ctaccacctc gaataccatg tggtgcagac cggtgccgtc atccgtgtcg
    19621 ccgatataca tacccgggcg cttacgcacc gcatccagcc ctttcaggac tttgatactg
    19681 gaggagtcat aagaattcga catcaacgtt tctcgctcat ttatacttgg gttaatccgt
    19741 tattttaccc ttttccacgg taaacatctt cgaattttcg tccgacatgt ctataacgtg
    19801 ttcagcactg atcgcgctga caaagacctg tgattgcgtc gcttttaagc ggctggcaag
    19861 cagcccgcga cgctcatcat caagctcaga ggcaaaatca tctatcaggt agagacaccg
    19921 ccgcccgctt tcacgggtga ggaactctcc ttgcgccaga cgtaaggcgc acatcaacag
    19981 cttaagctgc ccacgcgata aggtatcttc caccggcgca ccgtcggcgc gaatgcgtaa
    20041 gtccgcttta tgcgggccat gcgcggtgta ggttagctgg cgatcgcgtt caaaattacg
    20101 ttccagcacc tcagcatatt ctgtctcttt ctcccagccg cgctggaaag agaaagtcag
    20161 agaaaactca gggagaaatt gcttacaagt atcggccata tcagccgcga taccggcgct
    20221 atactccgcg cgccaggtgc tgatttgctc cgccagcggg atcagctctt tatcccacgg
    20281 gcgtagctgt tcgtaacgtg tcacctggcg cagcgccgca ttgcgctgct tgagcaatcg
    20341 cttgagattg ctccaggcgg tgaaaaatcc gggttcgttg tgaaagcatc cccagtcgag
    20401 gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta aacccttctg gcgttatcaa
    20461 ctgcattggc atcaggtgcg ccagttccgc gaccttatgc ccgtctgtac cgtcgatgcg
    20521 gactttgctg tcgccctgtt tgtctttggt taagccaatc gctgtctcgc gctcttcgcc
    20581 ctgtaatcgc ccgtggagaa caaacgcctc ctgctcatgg cgaatgacgc gaccaatctg
    20641 caaactgcga aacgcccgac catggccgag cgtatagatg gcttccagca cgctggtttt
    20701 gccactgccg ttggcaccta ccagaaagtt aaagccggga gataaagcga gatccgcggt
    20761 ttcaatgttg cggaaatcgc ggatcaacaa gcgggtgagg gacattacag tctcattggc
    20821 atgacaacat aggcagccga ctgtgatgcg gcatcttcaa tctgcacgct ggaaaccgaa
    20881 tcggtcagca tcatgcggac gttttcacat ttcagcgcgt tcagaacatc cagcacgtag
    20941 ctgacgttga agccgatttc catctccgca ccgctatagg taacgtcgag gatctcttcc
    21001 gcttcttcct gttccgggtt gttggcggtg attttcagct ggttttcgct gacataaaga
    21061 cgtacgccgc ggaatttctc gttagagaga atcgccgcgc gagcaaacgc ctgcttgagc
    21121 agatcgcagc cagcttccag atgtttgtcc gggttcttcg gcagaacgcg gcgataatcc
    21181 gggaagcgac catccaccag tttggaggtg aagataaagt cgccaacgtg ggcgcgaatg
    21241 ttgttgctgc caatctgtac gcgcagcgga ttgtcgccgc cgtcgagcat acgcatcagt
    21301 tcaatcacgc ctttacgcgg cacgatcacc gaatggcttg gcaaagattg accaattggc
    21361 attgaacaga ctgccagacg gtggccgtcg gtcgccacgg tgcgcagttc ttcgccttcg
    21421 gtttcaaaca gcataccgtt taagtaatag cgaacgtcct gatgcgccat agaaaactgg
    21481 gtcgcttcaa tcagacgctt catcgtcgcc tgcggcaggg taaattcgac ttcgctctgc
    21541 cagtcatcga ggttcgggaa atccgccgct ggcagggtag acagcgaaaa acggctacgc
    21601 ccggagcgca ccagcatccg ttcaccttcc agctgtacgg caatttccgc gccttcaggc
    21661 agaccacggc agatatcaaa gaatttgcgc gccggaacgg tcgtcgctcc tggctcgtgt
    21721 ggctgaacca gcgcaacgcg tgccaccatt tccatctcga gatcagtacc ggtcagcgac
    21781 aacgtaccgt cagcaacctg taacagcaga ttaccgagaa tcggtagcgt aggacgacca
    21841 cctaacggcc cgctcacctg ttgtagcggt tttaataaat gctcacgttc tacggtaaat
    21901 ttcataggtt tacgatgaca atgttctgat taaatttgaa aaatcttctt tgatatcgtg
    21961 gctctcttca cgcaactgct cgatcttacg gcaggcatga agcaccgtcg tgtggtcacg
    22021 accaccaaac gcatcgccaa tctccggcag actgtggtta gtcagctctt tcgccagcgc
    22081 catcgccatc tggcgcggac gcgccaccga gcgggatcga cgcttggaaa ggagatccgc
    22141 gactttgatc ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg tgaccagttt
    22201 ttcctgcaat gccagcaagt cgcgcagtgc ctcacgcacg aagtcgatgg tgatcgcccg
    22261 tccggtaaag ttggcgttag caataacgcg gttcagcgcc ccttccagct cacgtacgtt
    22321 agatcgtaga cgcttggcga taaagaacgc cacttcgcct ggcaaacgaa tgtcattttc
    22381 gtcggccttt ttcatcagga tcgccacgcg ggtttccagc tctggcggtt cgatcgccac
    22441 agtcagtccc caaccgaagc gggatttcaa acgatcctca acgccgttga tctctttcgg
    22501 atagcgatcc gaggtgagaa tgatctgttg attaccttcc agcagggcgt tgaaggtgtg
    22561 gaaaaactct tcctgagatc gttctttatt agcaaaaaac tgaatatcgt cgatcagcag
    22621 tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt gcagggcttt
    22681 aaccatgtcc tgaacaaagc gctcggagtg catataaacc actttggcat tcggcttgcg
    22741 cgccataatg ccgttaccca ccgcatgcag caggtgagtt ttacccagac ccgtgccgcc
    22801 ataaaggaac aatgggttat aggcaccgcc agggttatcc gccacctggc gagccgccgc
    22861 gcgcgccagt tggttagatt taccttcaac gaagttatca aacgtgtgtt tgacgtttac
    22921 gttagaacga taggtcggtt ctgccggggc cgggacgtta tcccaacctg agcgcgtaga
    22981 aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga cgttgctcgt
    23041 cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac gcagctgtgg
    23101 ggcatccgct ccgcagaaac tggttagcag tccattgata ttattaaggt acttgtcccg
    23161 tacccaatcg aggacaaaac ggtttggcgc gtacagggcc agcgtgttat cgctcagttc
    23221 cgcctgcaat gggcgtatcc acatactgaa ttctgtggct ggtaactcat cctgcaatcg
    23281 ggcaagacac tgctgccaaa gcgaaagtga cacggcggac tccactcgaa caaaagtcga
    23341 taatgactaa ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac cctgcttgag
    23401 ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc aagatcctgc aaaacgatcg
    23461 ggaccgcgga tcatagccta aactgcgcaa gagatcttct gtttctcaca gattttcccg
    23521 atttatccac aggactttcc agacctcgct aagtgtaaac gatcctgccg cgaggcgggc
    23581 acgatttacg ccgcatattg gaaaatttaa tgaccataga caaaaattgg cttaatcgat
    23641 ctaataaaga tctaggacga tccttgcgct ttacccatca gcccgtataa tcctccaccc
    23701 ggcgcgccat gctggttttc actggtgtga ggtcgtacat tttccctgcg aaaaggtgcg
    23761 gaaaagcgcg gtaaataagg aaagagaatt gactccggag tgtacaatta ttacaatccg
    23821 gcctctttaa tcacccatgg cttcggtgtc catcgtttca tttttcggcg gatatccaat
    23881 aaagccattg aatttattca agtttaggta gaaatcgcca tgaaacgcac ttttcaaccg
    23941 tctgtactga agcgcaaccg ttctcacggc ttccgtgctc gtatggctac taaaaatggt
    24001 cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt ttctaagtaa
    24061 taaagctaac ccctgagtgg ttaagctcgc atttcccagg gagttacgct tgttaactcc
    24121 cagtcaattc acattcgtct tccagcagcc acaacgggct ggcacgccgc aaattaccat
    24181 tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg ccaagaaaaa
    24241 cgttcgacgc gcccatgaac gcaatcggat taaacgtctg acgcgtgaaa gcttccgtct
    24301 gcgccaacat gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag gggttgccga
    24361 cctcgataac cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc actgtcgcct
    24421 ggctcgcggg tcctgatagc cctcattcgg gtctatcaac gcctgattag tccgctactc
    24481 gggccgcatt gtcgtttcac tccaacctgt tcaagctacg gaattgaggc attgcgcagg
    24541 tttggagtga taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg ccacccttta
    24601 caccctggtg gtgacgatcc cgtcccgccc ggaccatttg ataccagaga acactaacga
    24661 tggattcgca acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc atgatctggc
    24721 aagcctggga gcaggataaa aacccgcaac ctcaggccca acagaccacg cagacaacga
    24781 ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc cagtggccag gggaaactga
    24841 tctcggttaa gaccgacgtg cttgatctga ccatcaacac ccgtggtggt gatgttgagc
    24901 aagctctgct gcctgcttac ccgaaagagc tgaactctac ccagccgttc cagctgctgg
    24961 aaacttcacc gcagtttatt tatcaggcac agagcggtct gaccggtcgt gatggcccgg
    25021 ataacccggc taacggcccg cgtccgctgt ataacgttga aaaagacgct tatgtgctgg
    25081 ctgaaggtca aaacgaactg caggtgccga tgacgtatac cgacgcggca ggcaacacgt
    25141 ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt caacgtcaac tacaacgtgc
    25201 agaacgctgg cgagaaaccg ctggaaatct ccacctttgg tcagttgaag caatccatca
    25261 ctctgccacc gcatctcgat accggaagca gcaacttcgc actgcacacc ttccgcggcg
    25321 cggcgtactc cacgcctgac gagaagtacg agaaatacaa gttcgatacc attgccgata
    25381 acgaaaacct gaacatctct tcgaaaggtg gttgggtggc aatgctgcaa cagtatttcg
    25441 cgacggcgtg gatcccgcat aacgacggta ccaacaactt ctataccgct aatctgggta
    25501 acggcatcgc cgctatcggc tataaatctc agccggtact ggttcagcct ggtcagactg
    25561 gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca ggacaaaatg gcagctgttg
    25621 ctccgcacct ggatctgacc gttgattacg gttggttgtg gttcatctct cagccgctgt
    25681 tcaaactgct gaaatggatc catagctttg tgggtaactg gggcttctcc attatcatca
    25741 tcacctttat cgttcgtggc atcatgtacc cgctgaccaa agcacagtac acctccatgg
    25801 cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg ggcgatgaca
    25861 aacagcgtat cagccaggaa atgatggcgc tgtacaaagc tgagaaggtt aacccgctgg
    25921 gcggctgctt cccgctgctg atccagatgc caatcttcct ggcgttgtac tacatgctga
    25981 tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg gatccacgac ctgtcggcac
    26041 aggacccgta ctacatcctg ccgatcctga tgggcgtaac gatgttcttc attcagaaga
    26101 tgtcgccgac cacagtgacc gacccgatgc agcagaagat catgaccttt atgccggtca
    26161 tcttcaccgt gttcttcctg tggttcccgt caggtctggt gctgtactat atcgtcagca
    26221 acctggtaac cattattcag cagcagctga tttaccgtgg tctggaaaaa cgtggcctgc
    26281 atagccgcga gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa gggcggtcag
    26341 ttgaccgcct tttttctttt cgtagggtgg ataagcaccg cgcatccgcc acacaaagca
    26401 acaggaacat catgagcgat aatgacacta tcgtagccca ggccacgcct ccgggacgtg
    26461 gcggcgttgg catcctgcgc atctccggcc taaaagcccg tgaagttgcc gaaaccgtgc
    26521 tgggtaaact gcctaagccg cgctacgccg attatcttcc gtttaaagac gccgacggca
    26581 gcgtgctcga tcaggggatt gcgctatggt tccctggccc gaactcgttc accggcgaag
    26641 atgtgctgga actgcagggt catggcggtc cggtgatcct cgacctgctg ttaaaacgca
    26701 ttctgaccat tcccggcctg cggattgctc gccctggtga gttttccgaa cgcgcgtttc
    26761 ttaacgataa actcgactta gcccaggccg aggcgattgc cgatcttatc gacgccagtt
    26821 cggaacaggc ggcccgttcg gcacttaact cgctgcaagg cgcattctcc gcacgggtca
    26881 accatctggt ggaagccctc acccacttgc gcatttacgt cgaagcggca attgatttcc
    26941 ctgatgaaga gatcgatttc ctctccgacg gcaaaattga agcccagctt aatgacgtga
    27001 ttgccgatct cgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg cgcgaaggga
    27061 tgaaggtggt gattgccgga cgtcctaacg ccggtaaatc gagcctgtta aacgcgctgg
    27121 cgggccgtga agcagcaatc gtaactgata tcgccggaac cacacgtgac gtgctgcgtg
    27181 agcatatcca cattgacgga atgccgctgc atatcatcga taccgctggg ctacgtgaag
    27241 ccagtgacga agtggaacgt attggtatcg agcgcgcgtg gcaggaaatt gaacaggccg
    27301 accgcgtgct gtttatggtc gatggcacca caacagacgc cgtggatccg gcagagatct
    27361 ggccggaatt tatcgcccgt ctgccagcga aactaccgat caccgtggtg cgcaataaag
    27421 ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa cggtcacgcg ttaattcgtc
    27481 tctcggcaag gacaggtgaa ggcgtggagg tgctgcgtaa ccatctcaaa cagagcatgg
    27541 gctttgacac caacatggaa ggcggcttcc tggcgcgtcg tcgccaccta caggcgctgg
    27601 aacaggcagc ggagcatttg cagcaaggta aagcgcaact gttaggtgcc tgggcgggtg
    27661 aactgctggc ggaagagttg cgcctggcac agcagaactt aagcgaaatc accggggaat
    27721 ttacctctga tgatttgctg gggcggattt tctccagctt ctgtattggt aagtaaccgc
    27781 gcttacgaag ccgtattctg actgtcagat gcggcttcgc ttcattgtta gcactcctgt
    27841 tattcctcaa cccttttttt aaacattaaa attctt
//
LOCUS       DAJTXQ010000046        27457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000046 DAJTXQ010000000
VERSION     DAJTXQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.046"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..74)
                     /locus_tag="OO515_001888"
     CDS             complement(<1..74)
                     /locus_tag="OO515_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
                     /protein_id="HCR6575830.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDR"
     gene            complement(192..1631)
                     /gene="rsmF"
                     /locus_tag="OO515_001889"
     CDS             complement(192..1631)
                     /gene="rsmF"
                     /locus_tag="OO515_001889"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6575831.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1712..4345)
                     /gene="yebT"
                     /locus_tag="OO515_001890"
     CDS             complement(1712..4345)
                     /gene="yebT"
                     /locus_tag="OO515_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6575832.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4314..5597)
                     /gene="yebS"
                     /locus_tag="OO515_001891"
     CDS             complement(4314..5597)
                     /gene="yebS"
                     /locus_tag="OO515_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6575833.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5727..6224
                     /gene="msrC"
                     /locus_tag="OO515_001892"
     CDS             5727..6224
                     /gene="msrC"
                     /locus_tag="OO515_001892"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6575834.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6321..7019
                     /gene="proQ"
                     /locus_tag="OO515_001893"
     CDS             6321..7019
                     /gene="proQ"
                     /locus_tag="OO515_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6575835.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7039..9087
                     /gene="prc"
                     /locus_tag="OO515_001894"
     CDS             7039..9087
                     /gene="prc"
                     /locus_tag="OO515_001894"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6575836.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9279..10160
                     /gene="htpX"
                     /locus_tag="OO515_001895"
     CDS             9279..10160
                     /gene="htpX"
                     /locus_tag="OO515_001895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6575837.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10206..11269)
                     /locus_tag="OO515_001896"
                     /pseudo
     CDS             complement(10206..11269)
                     /locus_tag="OO515_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11446..12237
                     /gene="kdgR"
                     /locus_tag="OO515_001897"
     CDS             11446..12237
                     /gene="kdgR"
                     /locus_tag="OO515_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6575838.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12380..12619)
                     /locus_tag="OO515_001898"
     CDS             complement(12380..12619)
                     /locus_tag="OO515_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575839.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12778..12921
                     /gene="mgrB"
                     /locus_tag="OO515_001899"
     CDS             12778..12921
                     /gene="mgrB"
                     /locus_tag="OO515_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6575840.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12996..13283
                     /locus_tag="OO515_001900"
     CDS             12996..13283
                     /locus_tag="OO515_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6575841.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13953..14096
                     /locus_tag="OO515_001901"
     CDS             13953..14096
                     /locus_tag="OO515_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6575842.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14109..14318
                     /gene="cspE"
                     /locus_tag="OO515_001902"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14109..14318
                     /gene="cspE"
                     /locus_tag="OO515_001902"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6575843.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14484..15293
                     /gene="rlmA"
                     /locus_tag="OO515_001903"
     CDS             14484..15293
                     /gene="rlmA"
                     /locus_tag="OO515_001903"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6575844.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15290..15856)
                     /gene="mntP"
                     /locus_tag="OO515_001904"
     CDS             complement(15290..15856)
                     /gene="mntP"
                     /locus_tag="OO515_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6575845.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16285..16743)
                     /locus_tag="OO515_001905"
     CDS             complement(16285..16743)
                     /locus_tag="OO515_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6575846.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16798..17649)
                     /gene="manZ"
                     /locus_tag="OO515_001906"
     CDS             complement(16798..17649)
                     /gene="manZ"
                     /locus_tag="OO515_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6575847.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17662..18462)
                     /gene="manY"
                     /locus_tag="OO515_001907"
     CDS             complement(17662..18462)
                     /gene="manY"
                     /locus_tag="OO515_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6575848.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18525..19496)
                     /gene="manX"
                     /locus_tag="OO515_001908"
     CDS             complement(18525..19496)
                     /gene="manX"
                     /locus_tag="OO515_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6575849.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19959..21509
                     /gene="yoaE"
                     /locus_tag="OO515_001909"
     CDS             19959..21509
                     /gene="yoaE"
                     /locus_tag="OO515_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6575850.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21513..23111)
                     /locus_tag="OO515_001910"
     CDS             complement(21513..23111)
                     /locus_tag="OO515_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6575851.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23242..24606)
                     /gene="sdaA"
                     /locus_tag="OO515_001911"
     CDS             complement(23242..24606)
                     /gene="sdaA"
                     /locus_tag="OO515_001911"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6575852.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24790..25368)
                     /locus_tag="OO515_001912"
     CDS             complement(24790..25368)
                     /locus_tag="OO515_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6575853.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25372..26733)
                     /gene="pabB"
                     /locus_tag="OO515_001913"
     CDS             complement(25372..26733)
                     /gene="pabB"
                     /locus_tag="OO515_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6575854.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26807..26986
                     /locus_tag="OO515_001914"
     CDS             26807..26986
                     /locus_tag="OO515_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6575855.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27106..27429)
                     /locus_tag="OO515_001915"
     CDS             complement(27106..27429)
                     /locus_tag="OO515_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6575856.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7064 a         6767 c         6936 g         6690 t
ORIGIN      
        1 cgatcacccg gcgattcacc gtgtaattcg ccgtcgtcga tttcaaaagc gcctatgcgc
       61 acactggttt tcatcttcgt gctctccatt tagctgccgg ttgtggtacg accagtccga
      121 tcgcaccatt tttcagccta gttgaggcag ccgaatgtgc cagtcaaaaa gtgcgctttt
      181 tttgtgcgct gtcaggcgtt accggtaaaa agtttcccat cgcgcaccag ttcacgcgga
      241 tagctgtttt tcaatcgcga accaatccgt ttggctaaac caatcggttg atgctggaaa
      301 gtaaccaaca cgtcatccgc cactggcgcg gcttgcgggt aaacatcgcg cccgcgatac
      361 cactcctccg cttcctgcgg tgtcagttca aaagcgttca cattgtcggg ggtggcaagg
      421 gcaataactg cttcatgctg ccagcgataa cctttgttgt gcgtttcggc aagtttaatc
      481 cccaagcgag aaaatcggac tttaccgatc agggcttcaa tgcccaccgg gaacaaccac
      541 agttctttgt cacgctgcca gaggcgcagg ttttcatccc agtttaagcc aacacctgca
      601 gccgcctgac gaatttgtcc agcttctcga tctttcactg ggctgaacgg aaaattaccg
      661 actttgtatt tgggggcggg taaggcggga atcgcctgag ttttacgcag acgagcaacg
      721 aagaagcctt cgcagtcgta aatttgtggg aaaacatgca aaaagccttc ctcggtcagc
      781 gccttgtttg caccagggaa gagatcacca agcggtaaaa actctactgc gtcggggtaa
      841 gtctctttca gccacaggca aacggcttcg ttttcttcct ggtttaaggt acaggtcgag
      901 taaaccagcg taccgccagg gcgtaatgca tgaaaggcgc tgtcgatcag ttcccgttgg
      961 gtagctgcga tttcctgatt gctttctggt gaccagtttt ttagcgcatc gggatcttta
     1021 cgcaccacgc cttcgccgga gcagggagcg tccagcaaaa tggcatcaaa catttctggc
     1081 actgccgcac caaacacgcg gccatcaaaa tgtgtgagcg caacattact gataccacag
     1141 cggctgatat tggcatgtaa cacttttacc cgactggcgg aaaactcatt ggcaagaatt
     1201 gccccttcgt tattcatccg cgcggaaatt tgcgtcgttt tggagccagg cgcggcagcg
     1261 acatccatca cccgctgtgg tgcattaccg tcagcaaaca aggcggcgac gggcaacatt
     1321 gaactggctt cctgaatata aaacaggcca cttaaatgct cggcggtact acccaatggc
     1381 aatgcatctt cattgtcgcg ttcaatccag aaaccttctt cacaccacgg aattggcgta
     1441 agcgtccagc cataaggagc ggttaattgc aggaaatcag caacggagat tttcagcgta
     1501 ttaacgcgaa tgctgcggcg caacgggcgc tgacaggcgg caagaaaatc atcaaaagag
     1561 agcgtcgaag gcatcgcttc gcgcatttgc gtcagaaagg cgtccgggaa ataaacggtg
     1621 tgttgggcca cgagcatata ccactggcaa aaaaaacagg cgcgcagttt agcataaacg
     1681 ctccggcgca ggcacgccgg agcagtgggc attatttggg aagcgcagta ccccattcac
     1741 gccactcttt cggttcactt tcctgcaaca ggaagtgctt gccttcctgg gctttcggtg
     1801 ccagtggcgt acccggaggc gtggcgaagg cgatgccgcc acggataaac tggttaaagg
     1861 tgccggtttt cactacgccg cccgtcagac caaagtccag actgtaaccc gatgccaacc
     1921 agaagacgga attgttacgc accaggtgtt gatagcgttt actgatgcgc atcgcaatca
     1981 tcacgcgatc tgacaatgtc cccagcgtca ttccggtaac cgtacctact tccagaccac
     2041 ggaacagcac aggcgtacca atacctaacg aaccggcttc cggtgcttcg acaataatgc
     2101 ttaagccatc caggtaacgc gaatcagtaa tagtagcttc ttgcaactca aagtcacggc
     2161 gaggattacc ccgacctggt tcgacattga tatacggctg gaggatagta tctagatgct
     2221 caacgcccgc ggctgaaatt tgcggtgtga ccaccgagaa gcgcgtaccg ccgcgggcga
     2281 aggtctggac atactccgga tagagcaccg cctttgcctg tacttcattg cgcgcggtaa
     2341 tcagatccag cgtctggatt tgcccgatat caataccgag atagcgaatt ggcatcccga
     2401 ccgccagttt tccggcatcg aaagcgtgaa gcgtaatctg cccaccaacc gcacgggccg
     2461 ctgtttcgga agcatacaga attcgtttgt cgcctttacg ctgactggcg ctggcaccgc
     2521 tgaggttatc gaagctaatg gctcccttta atgctctgga gagcggggat gcctgtacgg
     2581 tcaggccact accattcagc tgaactttcg ccccgccttc tgcccagaac acgctattgc
     2641 tggtcagaag gttgcgatac tccggcttaa tatgcagatc gatatcaaac gcgttagctc
     2701 gcggacgcac ggtaatcact tcaccaactt caaatttacg gtacagcact accgatcctg
     2761 cctgcacatc cggcagcgtc tctgcactca aactcacggt tgtggtgggt aaatcgctaa
     2821 ggctgttctc cagcgctttt tccagattgg catacagtgg atagctggct ttcatctcac
     2881 ctttatcgcc tggcagaata cgtatcccgc cgttaatcca ttctgaggcg ctggcaccaa
     2941 gaaactcaac gccatccagc cccaccttca cgtcgacacg gctgttgacg acaaatttgc
     3001 tatcgccttt taccagttcg cgatgctgag gctcgatggc gacggtaaag gtaacgcctt
     3061 tgctggtgag tttacgatca ataacctggc ctacctgcac gccgtgaaga atgagcggct
     3121 gaccagcatc aataccgtaa ctttccggcg cggtcagggt cagcgtcaga acatcaggtt
     3181 catgcagcag tgctttttcg cctggcacaa caacgaactc tttgcgtggc tcgccatcgc
     3241 cgggtaccaa ctcgaaggtt ttgccagtca gcagggcgct gagattggca tcgctaaggg
     3301 ataatttcgg attaagtaat tcgatgcggg tattttcacg aagcagggta acgacgctgg
     3361 gattaactgt catttcccca gtgactttac caccaggatt taaatccagt ttagttagct
     3421 gtccgacttc cagtccctga tacattaatg gcgtcgagtc ggcggttaat ccggccccac
     3481 tcggcagttc cagtttgatt attacgccac gctggctatg ggcgaggtct tcatacagac
     3541 caaaggtatc ttccgcctcg gcaggtttcg actcttcagg tgaatcgaag gcaatcgcac
     3601 cgttaaccag tgccgccaga ctttccagtt tcaccttcgc accactgata ctgacgttgg
     3661 catcaacgcc ggaaacgttc cagaaacggc tacctttttt cactaggtcg gtaaaacgcc
     3721 gctcgatcag gacatcaatc accacgcctt gcttgttggg attgatggca tagtcgtaga
     3781 cttttcccac agggatcttg cggaaataga ccaatgaacc gctgttcagc gaaccgagat
     3841 cgggggcttg caggtggatc atcagatcgc cattgtccag tcgatatttc ggttgggtat
     3901 cgagtgcgac aaagtgatcc tgctctttac ctttacctgg catcatgccg atatagttac
     3961 caccgacgag ggcgtccagc ccggagacac ctgccaacga tgcttttggc gtcaccagcc
     4021 agaactgagt ctcttcgcgc agcgcatctt tcatatcgga cttgatgctg accttgactt
     4081 caatcttacg aagatcgtcg ctgaggctga tatcctgcac tgttccgact tcaacgccct
     4141 gataacgaac aggcgtacgg cccgggacaa taccatccgc cgacataaag tcgatggtga
     4201 cggtattacc ccggtcctga taactgtccc aaatcagcca accggcaatc attagcgcga
     4261 tgaaaggcag cagccagaaa ggtgagatac ggcgtttatt tttaatctgc gcttcagtcg
     4321 tcgaagcggg cgtttcctga ctcatgtgca tcccaaagta agcggctgtc cagccattcc
     4381 acagcaagaa tagtcaaaat tacggctgcg ccaaaataaa acgcagccgg tcccatagta
     4441 aaagcgagga tctgatcgcg attaatcagc gacatggtta aagatatgac aaacaggtcg
     4501 agcatcgacc agcgtccaat ccaggtcacc atccgcagta acagaatgcg tgtgcgtaaa
     4561 ccttgctggc atttaaaatg aatgctcaac agtaaagtga acatgacgat cactttagta
     4621 aacggtacca gaatactggc gataaacacg attccggcga ccgcaatgtt gctacttgcc
     4681 agcgacataa tcccggaaag aatcgtatct tcctgccggc caccattcag ataaatgata
     4741 gaaataggca ataggttggc tggtaacaac aaaacgattg acgctaacag cgccgcccag
     4801 catttttgca aactatgacg acgacgcagg cgtagcggga tatggcaacg cgggcagcga
     4861 ccacgctgat caggatagcc ggtaaaatgg cacccaagac agacacgaag tttctcgtcc
     4921 ctacgcgtag cggggcgctg cggataatat cgctcccaca gctcctcaac attaagatgt
     4981 gacaacgtca ccgtcgttaa aatcaccaac gccacaaaag agaacaagcc gacacccgcc
     5041 tggatatggg cataatcctg tacctttata gaagcaacgc caatgccgac caggtagata
     5101 tccagcatta cccactcttt aagacgctca agcatcagca gtaccggacg taaattcatt
     5161 cccagtcggt taccaaacca taaatatgct atggaggaca ccagaatgag gggggcacca
     5221 ataacgcaga aaaagaccat cgcccccgtt atcgtatcgc cctgtttggt catttgccag
     5281 atgccttgca tgacgttggc gtcgatacga atacctaaca gccagatatg caacagcggt
     5341 tcgccccagg caaacggcat cagcaacagc atggtgaaag ccattgccgc caggcgcgtt
     5401 agcgaccagt cgcgcccgtc gcgaattttt gcctgacagc gcggacaata ggcactctga
     5461 tgagaattta tctcgggcag gctaaacagc atgtcacatt gcgggcaacg ttggtaatca
     5521 ccacgcggca gttcctcgcc gattgccctc actgttatct tttttgtcgg cgtaatttgt
     5581 ggtgtgttaa gagccatgac gtgccaaatg taataagaat taacattaat cttaacccat
     5641 gatgggaatg atcttgagca ttaacgcatt taatgcttat tttaataggc tggcagttcc
     5701 aggtaagaca aacaagtgat tatataatga acaaaacaga attttacgcg gatttaaatc
     5761 gcgactttaa cgcgctgatg gcgggagaaa ccagttttct ggcaacgctt gcgaacacca
     5821 gtgcgttgtt atacgagcgt ctcactgacg taaactgggc aggtttttat ttgcttgagg
     5881 acgatacact ggtactcgga ccatttcagg gcaaaattgc ctgtgtccgg atacccgtcg
     5941 ggcgcggggt gtgcggcact gcggttgccc gcaatcaagt gcagcgtatc gaggatgttc
     6001 acgcgtttga cgggcatatt gcctgtgatg cggcgagtaa ttctgaaatt gttctgccgc
     6061 tggtggtgaa aaatcagatt attggtgttc ttgacatcga tagtaccgtc ttcggtcgct
     6121 ttacagacga ggacgagcaa ggcttacgtc agcttgtggc acagcttgaa aaagtgcttg
     6181 caacgacgga ttacaaaaaa ttctttgcga gcgtcgcagg ataatcaacg gataacgtag
     6241 catttactgg tagcgtcatt ataatgacgc ctgttcatgc ctgcgcttgt tggctacgtc
     6301 cgttgtaatc aggaaatttc atggaaaatc aacctaagtt gaatagcagt aaagaagtaa
     6361 tcgcgtttct ggccgaacgt tttccccact gtttcagtgc ggaaggtgaa gcgcgtccgc
     6421 tgaaaatcgg tatttttcag gatttggtcg atcgtgttgc cggggaaatg aacctgagca
     6481 aaacgcaatt acgatccgct ttacgtctct acacttcgag ctggcgttat ctttacggtg
     6541 ttaaacccgg cgcaacgcgt gtcgatcttg acggcaaccc atgcggtgag ctggacgagc
     6601 aacatgtaga gcatgctcgc aagcagcttg aagaagcgaa agcgcgtgtt caggcacagc
     6661 gtgctgaaca gcaagcgaaa aaacgcgaag ctgccgcagc tgctggtgag aaagaagacg
     6721 cactgcgccg cgaacgcaag ccacgtccga ccacgccacg ccgcaaagaa ggcgctgaac
     6781 gtaaacctcg tgcgcaaaag ccggtagaga aagcgccaaa aacagtaaaa gcacctcgcg
     6841 aagaacagca caccccggtt tctgacattt cagctctgac tgtcggacaa gccctgaagg
     6901 tgaaagcggg tcaaaacgcg atggatgcca ccgtattaga aatcaccaaa gatggcgtcc
     6961 gcgtccagct gaattcgggt atgtctttga ttgtgcgcgc agaacacctg gtgttctgaa
     7021 acggaggccg ggccaggcat gaacatgttt tttaggctta ccgcgttagc tggcctgctt
     7081 gcaatagcag gccagacctt cgctgtagaa gatatcacgc gtgctgatca aattccggta
     7141 ttaaaggaag agacgcagca tgcgacggta agtgagcgcg taacgtcgcg cttcacccgt
     7201 tctcattatc gccagttcga cctcgatcag gcattttcgg ccaaaatctt tgaccgctac
     7261 ctgaatctgc tcgattacag ccacaacgtg ctgctggcaa gcgatgttga acagttcgcg
     7321 aaaaagaaaa ccgagttagg cgatgaactg cgttcaggca aactcgacgt tttctacgat
     7381 ctctacaatc tggcgcaaaa gcgccgtttt gagcgttacc agtacgcttt gtcggtactg
     7441 gaaaagccga tggatttcac cggcaacgac acttataacc ttgaccgcag caaagcgccc
     7501 tggccgaaaa acgaggctga gttgaacgcg ctgtgggaca gtaaagtcaa attcgacgag
     7561 ttaagcctga agctggcagg aaaaacggat aaagaaattc gtgaaaccct gacacgccgc
     7621 tacaaatttg ccattcgtcg tctggcgcaa accaacagcg aagatgtttt ctcgctggca
     7681 atgacggcgt ttgcgcgtga aatcgacccg cataccaact atctttcccc gcgtaatacc
     7741 gaacagttca acactgaaat gagtttgtcg ctggaaggta ttggcgcagt gctgcaaatg
     7801 gatgatgact acaccgttat caattcgatg gtggcaggtg gtccggcagc gaagagtaaa
     7861 gctatcagcg ttggtgacaa aattgtcggt gttggtcaaa caggcaagcc gatggttgac
     7921 gtgattggct ggcgtcttga tgatgtggtt gccttaatta aagggccgaa gggcagtaaa
     7981 gttcgtctgg aaattttacc tgctggtaaa gggaccaaga cccgcactgt aacattgacc
     8041 cgtgaacgta ttcgtctcga agaccgcgcg gttaaaatgt cggtgaagac cgtcggtaaa
     8101 gagaaagtcg gcgtgctgga tattccgggc ttctatgtgg gtttgacaga cgatgtcaaa
     8161 gttcaactgc agaaactgga aaaacagaat gtcagcagtg tgatcatcga cctgcgtagc
     8221 aatggcggtg gggcgttgac cgaagcggta tcgctctcag gtctgtttat tcctgcgggt
     8281 cccattgttc aggtccgcga taacaacggc aaggttcgtg aagacagcga taccgacggg
     8341 caggtgttct ataaaggccc gctggtggta ctggttgacc gtttcagtgc ttcggcttca
     8401 gaaatctttg ccgcggcaat gcaggattac ggtcgtgcgc tggttgtggg tgaaccgacg
     8461 tttggtaaag gcaccgttca gcaataccgt tcattgaacc gtatttacga tcagatgtta
     8521 cgtcctgaat ggccagcgct gggttctgtg cagtacacga tccagaaatt ctatcgcgtt
     8581 aacggcggca gtacgcaacg taaaggcgta acgccagaca tcatcatgcc gacgggtaat
     8641 gaagaaacgg aaacgggtga gaaattcgaa gataacgcgc tgccgtggga tagcattgat
     8701 gccgcgactt atgtgaaatc aggcgattta acggcctttg ggccggagct gctgaaggaa
     8761 cataatgcgc gtatcgcgaa agatcctgag ttccagaaca tcatgaagga tatcgcgcgc
     8821 ttcaacgcta tgagggacaa gcgcaatatc gtttctctga actacgctgt gcgtgagaaa
     8881 gagaataatg aagatgatgc gacgcgtctg gcgcgtttga acgaacgctt taaacgcgaa
     8941 ggtaaaccgg agttgaagaa gctggatgat ctaccgaaag attaccagga gccggatcct
     9001 tatctggatg agacggtgaa tatcgcactc gatctggcga agcttgaaaa agccagaccc
     9061 gcggaacaac ccgctcccgt caagtaatat caatcaggca caagaaattg tgcctgattt
     9121 tttaacagcg gcaagatgcc gtaaatcaga tgctacaaaa tgtaaagttg tgtctttctg
     9181 gtgacttacg cactatccag acttgaaaat agtcgcgtaa cccatacgat gtgggtatcg
     9241 catattgcgt tttgttaaac tgaggtaaaa agaaaattat gatgcgaatc gcgctcttcc
     9301 tgctaacgaa cctggccgta atggtcgttt tcgggctggt actgagcctg acagggatac
     9361 agtcgagcag cgttcagggg ctgatgatca tggccttgct gttcggtttt ggtggttcct
     9421 tcgtttcgct tctgatgtcc aaatggatgg cattacgatc tgttggcggg gaagtgatcg
     9481 agcaaccgcg taacgaaagg gaacgttggc tggtcaatac tgtagcaacc caggctcgtc
     9541 aggcagggat cgctatgccg caagtggcta tctaccatgc gccggacatc aacgcttttg
     9601 caaccggtgc gcgccgtgat gcctctctgg ttgctgtcag caccggtttg ctgcagaaca
     9661 tgagcccgga tgaagccgag gcggtaattg ctcacgaaat cagccacatc gccaatggtg
     9721 atatggtcac catgacgctg attcagggcg tggtgaacac cttcgttatc tttatttccc
     9781 gtattctggc gcagcttgcc gcgggtttta tgggcggaaa tcgtgatgaa ggtgaagaga
     9841 gcaacggcaa cccgctgatc tactttgcgg ttgcaacggt tctggaactg gtgtttggta
     9901 ttctggcgag cattatcacc atgtggttct cgcgtcatcg tgaattccat gctgatgccg
     9961 gttcggcaaa actggttggt cgcgagaaaa tgattgccgc gctgcagcgc ctgaaaacca
    10021 gctatgaacc gcaagaagca accagcatga tggctttctg cattaacggt aagtcgaaat
    10081 cgctcagtga gttgttcatg actcacccgc cgctggataa acgaattgaa gctctgcgta
    10141 cgggtgaata cctgaagtaa tatgaagtaa atactaaaaa gcgcgtcctg atcgacgcgc
    10201 tttttttatg ccttggatcg tggctgagtg atacgtaaac cactgacaca ggcagcaatc
    10261 actgccagaa tagccgcagc catcagcgaa acgtgcgtac cattatcacc gaactgattt
    10321 agcatcagcg ccaccagtgc cgcgccgcta ctctgaccca gtagacgagc cgttcctaac
    10381 atgccactgg ctccaccgct acgttcgcga ggagcggagg taataatggt gtggttattg
    10441 ggtgactgga acaagccaaa tccagcgcca cataagatca tcggccagat aatattgata
    10501 tccgcaggtg acgcgggcag cagaaccagg ggaaaaagcc ccgcagccat gatgaacaac
    10561 cctagagcac ccagcaatcc tgcatgtacg cgttcaatca aatagcctgc caacggagcc
    10621 atcaccatcg ttgctaacgg ccacggtgtc agaagtaaac ctgtttcgac ttcactacgc
    10681 ccgagcacgg tttgcaggta aaagggcagg gaaaccattg ccagcatttg tgcgcagaaa
    10741 gagcaaacag atgtgcaaat agaaagtgaa aacagcggga tacgcagtaa atccaccggt
    10801 agcagcggta cgggaagaga aagctggcgg cgaataaaga aaataccaac aacaaccatt
    10861 accaccagtt ccgcagcaat taacgtcagc gattgcccct gagcgaaacc actcaacgca
    10921 gtgataagca ggccgaaggt taacgcgttc atcacggcgc tgggcaggtc gaaacggggt
    10981 ttactggcgc gagaaccatt gggtggcaga aaacgcatcg ccagaagcag ggcgataata
    11041 cctaacggta cgttgattaa aaataaccat ttccaggatg cgatggagag gattgctgca
    11101 gcaattgtcg gcccggcagc agaagagacg gcaacaatat ggcgattgtt ggcagggcga
    11161 cgttggcgat tgcgccgtca aggacagcca tcgaaatgcc aatcacaatg gttaatatcg
    11221 caccgtatcg ctggggcaat ggcaggccgt cggcctgaac ttttggcata ggaattttat
    11281 atctttggtg aataatcaac aggggtatga ttctagcact gatattcacg gtggatgtcg
    11341 cagaattgta actaaccggc aaaagatgga ttgcgaccac cctggtgcga aattataata
    11401 aaaaacagtt ctgattttta taaaacactc gcaatgaggt gataaatggc taacgcagat
    11461 ctggataaac agcctgattc tgtatcttcc gtgctaaaag tttttggcat tttgcaggcg
    11521 ctgggtgaag agcgcgaaat agggataacc gagctgtcgc agcgcgtcat gatgtcaaaa
    11581 agcaccgttt atcgcttttt acagaccatg aaaaccttag gttatgtggc gcaggaaggg
    11641 gagtcggaga aatattccct gaccctgaaa ttgtttgaac tgggcgctcg cgcgttacaa
    11701 aacgtcgatt taattcgtag cgcagatatc cagatgcgtg agatctcccg cctgaccaaa
    11761 gaaactatcc acctcggcgc actggacgaa gacagtattg tttacattca caaaattgac
    11821 tctatgtaca atttgcgcat gtattcacgg attgggcgtc gtaatccgct gtacagcacc
    11881 gcgattggta aggtactgct ggcatggcgc gatctcgatg aagtgaagca aattcttgag
    11941 ggcgtggagt ataaacgcag taccgagcgg accatcacca gtacagaagc gttattaccc
    12001 gttctggacc aggtgcgcga gcaggggtat ggcgaagata acgaagagca ggaagaaggg
    12061 ctgcgatgca ttgcggtacc ggtatttgat cgctttggcg tggtcattgc cggtttgagc
    12121 atctccttcc cgacgttgcg tttctctgaa gagcgtttac aggaatatgt cgcaatgttg
    12181 cataccgcag cgcgcaaaat ttctgcccaa atgggttatc acgactatcc gttctgatga
    12241 gagtaagaac ctgtcggaat atcaaacaga caggttcttt atttagcatg agaaaaataa
    12301 agttgaaggt gcgttatatt aaacgcgctt gctataagaa tattttactc atgagtgaga
    12361 atctggttgt ttattaccct taaccattat cgaccacgat attgctttta cgtaacagtg
    12421 ggcaatctgt tatccccaag aaaccacttt tagtgtgcaa gtattgtacc gtgctggtgc
    12481 ctctggcagt cagataggta cattgcaaac ctaatcctgc ggcattctct ttgcttccaa
    12541 tcaaaacgcc atatccgctg agtaataatc ctatccatac cagtgctatc agcataactg
    12601 tgcgaatgat gaatcgcatt acaacctctt ctctttttat gttcgcttaa tcgtagcggc
    12661 aatatgcgct gaagcaagct actcattccg aaaaagcacg aatatcgaca tagttaggcg
    12721 ctgtttaact aacgcatgct agtttaatga cataaggtag gtgaagcgga gattggagtg
    12781 aaaaagtttc gatgggtcgt tctggttgtc gtggtgttgg cttgcttgct gctttgggcg
    12841 caggtattca acatgatgtg cgatcaggat gtacaatttt tcagcggaat ttgtgccatt
    12901 aaccagttta tcccgtggtg atatcatttt ttcagcacta gattttcttc ctctccagca
    12961 gtggtagaat agcgcctcta ctcagaggtg gtgaaatgaa cgaagttgta aattcaggcg
    13021 tgatgaacat tgcgtctttg gttgtatcgg tggtggttct tcttatcggg ctcatcttgt
    13081 ggttttttat caatcgtgcc agttcacgga ctaacgaaca gattgaactg cttgaggcgt
    13141 tgctggatca gcaaaaacgt caaaatgcac tgttacgtcg tttgtgcgaa gcaaacgaac
    13201 cagagaaagc agataaaaag accattgaga gtcaaaaatc ggttgatgac gaagatatta
    13261 ttcgcctggt cgccgaacga taatccttcc tcaatcttga gtaaccccgt cgttgaaggg
    13321 ttactcaatt catcctgatt atacgcactg tcatggtgca actgcttcaa attaaaactc
    13381 tggtctgact tcaaataatt atcatattat ttatttgcgt aacgggatat cagaaaaaga
    13441 tattttcctc attaatttat taggatgttt acatcggatt tgtgattaag cgtggtatta
    13501 tttattagcg aaacgtttct ctcttgattt ttttgctcat tcatcaattt ttcttatttt
    13561 aaattttcaa tccctttggg gattgacttc tctttagggt aattgatagc cgttaaccga
    13621 ctgttttatg agaaaaagtg atataacttt ttattcattg catagcaaaa aatgtgatat
    13681 tgcacgcact atgtaataac ttctcccact ggcctggaac aactgaactt attgaactat
    13741 gttagaaaat acgccagttt aagtatctgc ctgaactggc aaggttaagc acaatgatat
    13801 atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt
    13861 gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta tgtgataaca
    13921 gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt aaagaagtcc
    13981 tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt ggtgcctcat
    14041 gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca atctaaggaa
    14101 tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct aaaggttttg
    14161 gcttcattac tccggctgat ggcagcaaag acgtgttcgt acacttctcc gctatccagg
    14221 gtaatggctt caaaactctg gctgaaggtc agaacgttga gttcgaaatt caggacggcc
    14281 agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc gaatccactg atctgaagtg
    14341 tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt actatgcttt
    14401 atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac acttgcggcc
    14461 tttagtatcc tgccggagtt gtcatgtctt tttcctgtcc actttgccat cagcctcttt
    14521 cgcgtgaaaa aaacagctat atctgtcccc agcgacatca gtttgatatg gcgaaagaag
    14581 ggtatgtcaa tctgctgtcc gttcagcata aacggtctcg tgatccgggc gacagcgcgg
    14641 aaatgatgca agcacgccgc gcattcttag atgccggaca ttatcagccg ctgcgtgatg
    14701 caattgtcgc ccaactgagg gaacggcttg atgataaggc cacggcggtg ctggatattg
    14761 gctgtggtga agggtattac acacacgcat ttgccgatgc gttgcccgaa atcaccacgt
    14821 ttggtctgga tgtttcgaag gtagcgataa aagcggcggc gaaacgctat ccgcaggtca
    14881 ctttttgtgt cgcttccagc caccgtttgc cgttttccga taccagtatg gacgccataa
    14941 tacgtattta cgcgccgtgt aaagcagaag aattagcacg agtagtgaag cccggcggct
    15001 gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg ctgatttaca
    15061 atgaagtaca tcttcatgca cctcatgcag aacaactgga aggttttaca ttacagcaga
    15121 gtgcggagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca ttattgcaga
    15181 tgacgccgtt tacctggcgt gcgaagccag aagtctggca aacactggca gcaaaagaag
    15241 tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat taaccgtgga
    15301 agtgcgtcca gaggatctgg acgccgatgc cgatcagtac cagcccgccg agaatttccg
    15361 cttttttccc aataattgag ccgataaagc gaccaaccat catccctaat gttgacataa
    15421 tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctgc aggaaagcaa
    15481 gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt accagtagcc
    15541 agaaaccgtg tcgacggcgc ggctcttcat cttcatcatc tgcgccacga aaaccctcaa
    15601 taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtgg ttccattcaa
    15661 ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggcgtc agggtttcga
    15721 cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta tggagggtgg
    15781 cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca agaagaacag
    15841 tagcagtgat attcatgaca atgtcctgac cggggtatcc ataaaacaca tcatcgcccc
    15901 cagcaaacat gcatacatga acaacaagcc gtgatggttt tcgcgttcat gcaggcatag
    15961 cgattgatgt gcatatggtc tcgcctgact gaaagtattc agtccgtacg tgccacgttt
    16021 tgcaacgagt atgttgacac gtacatttcc ggaatctgga aatcggctac tccccaaatg
    16081 agggcgcaac cttaacatat tttgagaata taaaacaact gttggaatat attatttcat
    16141 tgctttattg ataacgattt tcatttggat ttatatatca atgaaacgtt aaaataaata
    16201 acaaataagg gaagaggtaa tatagcctaa gctatgtctg attattaaaa atacactaaa
    16261 catagctttg gctaaattca taacttattg agttgaaacg agaagcttat aaattttttc
    16321 cagatcgtcg atatttcgaa cgcggattaa cagccgacgc tgttctaatt gcatcaccaa
    16381 cacgccatct tccgacaagt tcatcgcttt gattcggcta tattcaatcc agacattggc
    16441 gaagaaaaaa cctttttgtt taaagatgat cttcggaacg cggatccaga atatataaaa
    16501 acccatcaga gccagtgcgc ttaataacca tgtcgttatt aacgcaccat gattcgtgac
    16561 gttgttatag ataagaatga caatcaatcc gacgaagata acgctatcga tgcgaccacg
    16621 ccggagcaaa ggaattgcca gcagggtggg gccgttacgg cggggcatga tgaactgatc
    16681 gtagatcgcg aaggccagga gtgcggcgat gaaaagaatc agtaccaggt ccgtgattgt
    16741 cattaatcct ccagataaaa aaacggggcc aaaaggcccc ggtagtgtac aacagtctta
    16801 cagtcccagc aggccgcaag cgtaaccagc gataccgatg acgaagaagc caacgatgat
    16861 ccacagcggg ttaacttttt tgcgcagtag ccacatacaa gcaaaggtca gcagcagtgg
    16921 taccaggcct ggcattaact ggtccagaat agtctggaca gtagtaacgt gttctttgcc
    16981 cgtctggtca gtaatgcgag agacaaccag cgggatgttg acatgtgtcc acttgttaac
    17041 caatgccccc atgacaaaca ggccgaggat agacgcccct tccgtcagtt tttgcaggaa
    17101 gccaccaccc atatctttaa cgatatcgat acctttggag taaccatacg ctacgccgta
    17161 gtaacgggtt gccagacgca ccaggttaaa caggatgaag aacagcagcg gacctaacag
    17221 gctgccgctc atcgcgatac cggcacccag tgctgcaaat accggacgta cggttcccca
    17281 gaagatcggg tcgcctacac cagccagcgg ccccatcaaa ccgactttga taccgttgat
    17341 agcaccgtcg tcgatctctg cgccattagc acgctgttct tccagcgcca gggttacgcc
    17401 gagaatcggc gcagccacga acggctgggt gttaaagaac tccaggtgac ggcgaatagc
    17461 ttgtttacga gcttcgttgt tctcagggta gaggcgacga attgccggta ccatagagaa
    17521 gcagaaaccc agtgcctgca tacgttcgaa gttccatgaa ccctggaaga ggttagaacg
    17581 caggaagacg ccacgaatat cactttgagt gagttttttc tcggtggtag tttgagttgt
    17641 atcaaccatt tcgctcacct gttagtccag ttcgttatcg agatcgttgt taccagctgc
    17701 ctgagcaggc gcaccggcta cgcggttgta tttcgggcta agttggatgt agagcactgc
    17761 cataacagta ccaatcacac ccagagcaac caggttaaag ttggtgaatg ctgcggttac
    17821 gaagccgagg tagaagaacg gcatcaggta gccagcacgc atcatgttga taaccatcgc
    17881 ataaccaacc accacgatca tgccaccggc gatattcaga ccattggtca ccacttccgg
    17941 aatcgcattc agcatgttct gtacttcgct ggtaccaaca gacagcgcaa cgatgacggc
    18001 cggaatagcc acacgcattg cttgcaggaa cagagaagaa acgtggatcc aggaaatcgc
    18061 tgtcaggttg ccgttatcag cagccttatc cgcagcgtgc tggaaagcaa cggtaatagt
    18121 acgaacgatg atggtcagta cctggcccgc agcggccaga gggattgcca gtgcgatacc
    18181 tgcaccaatg ctctgatgac ctgcgataac cagaatggta gaaatgatag aagccagagc
    18241 ggcgtcaggc gcaactgcag caccgatgtt catccagccc agcgcgatca tttccagcgt
    18301 accaccgata ataataccgg ttttcatatc cccaagaacg atacccacca gggtacacgc
    18361 gattagcgga cggtgaaact gaaattcatc gaggattgat cccatacctg cgatacaggc
    18421 tacgataaat accagcacaa tttgaagagt ggtaatctcc attgtacttc tcctgtttac
    18481 gtaagactta agtgtgaaaa ctgagtgata atcaacacaa tacgttactt atcgattttg
    18541 ctgatcagat ccatcatttt cagtttcgga tcggtggaaa ccttacggac ttccagctca
    18601 ataccgcgcg cattcagttt cttgaacgcc tcgatatctt tttcatcaac cgaaaccgcg
    18661 ttattcacct gggttttacc ctgacggaat gccataccac cgacgttaac agaggtgatt
    18721 ttcacgccgc cttcaacgag acgctctaca tctgttgggt tggtaaataa cagcattacg
    18781 cgttcgccag catatttcgg gttgttgtag acgcgaatca ttttggcaac atcaactacg
    18841 tgtgctgtta cgcccggagg tgcaacctgg gtgagcagtg tcttacgaac ggtatccgca
    18901 gccacttcat cactaacaac aataatacgg gagacattgg tttctttggt ccagcgggtg
    18961 gcgacctgac cgtgaatcag acggtcgtcg atacgcgcaa ggccaataac catgtagtcg
    19021 tttggcccca ttggttttgc cggagttgga gccgcttttg gtgctgcggc agcgggtgct
    19081 ggcgcggctt tttcaaccgg tttggctttc agtgctttca cgccttcacg gcctgtttct
    19141 actgccagcg caaccagttc atcaaagctt gggtcatcat cacgagccat taacgtttcc
    19201 acgagcattg gaatgttaac gcctgcaatg acttcataat gctctttgtc gacgacaatg
    19261 cggctggcag cattgaacgg gctgcctccc catgtatcaa cgagaaacag cacgccttta
    19321 gtggtgtcga gttttgccaa ctgagcgttg tacttttcaa tcagcgtttc ggcattttca
    19381 cctggaacga aatcgatcca gccgacgttt tcctgctcgc ctaacagcat ttctgccgtt
    19441 ttaagcaact gctctgcagc ccaaccatgt gtgcctataa caatagcaat ggtcacttgc
    19501 tacctccttt attatcgtta acacctcaac gtgccagatg tatttttgaa tcgtcgtcca
    19561 caatcgaatc gattcagata agggctacaa ccaaaaaccc ctgcgtttcg cgatttattt
    19621 tagatatcga aaaaattatt ttatgtgatg aagatccgta atttaacttt cgattagcag
    19681 aaatttcgaa aggtaaaata tccttgtcac attcgtttgc aaaggaaggt aaatctttgc
    19741 caaatcagag gcgtctctga tatgtttaac tcccgtttaa caaccatgga gtatagggca
    19801 gtagcccgca gtatggatcg tcaccgacgt catttcagca tcaggccttt taacgcctgc
    19861 ctttctggca ctctatgccg cacctttcgt ttgcattttg tcgttacgcc tgcattattt
    19921 ctggcgtcga atagctattc cttaagcagg agcttgtcat ggaattctta atggacccct
    19981 caatttgggc ggggctactc acgcttgttg ttctcgaaat tgtgctgggt atcgataacc
    20041 tggtcttcat cgccattctt gctgacaaac tgccgccaaa acaacgcgat aaagcgcgtt
    20101 tgctggggtt atcactggcg ctgattatgc gtctggggct gctgtcgctg atttcatgga
    20161 tggtcacgct gaccaaaccg ctatttaccg tcatggattt ctccttctcc ggacgcgacc
    20221 tgattatgtt gttcgggggg atattcttgc tgttcaaagc aacaaccgaa ctgcatgaac
    20281 ggctggaaaa ccgcgatcat gattccggcc acggtaaagg ctacgccagt ttctgggtgg
    20341 tcgtcacaca gattgtcatc cttgacgccg tcttctcgtt ggatgcggta attactgcag
    20401 tagggatggt taaccatctg ccggtgatga tggcggcggt agtgattgcg atggcggtta
    20461 tgttgctggc atccaaaccg ctgacgcgat tcgttaacca gcaccccacg gtggtggtgc
    20521 tctgtctgag cttcctgtta atgattggtc tgagtctggt ggcagaaggt ttcggtttcc
    20581 acattccgaa aggttacctg tatgccgcga ttggcttctc gatcatcatc gaagtgttta
    20641 accagattgc gcgtcgcaac tttattcgcc accagtcgac tttgccgctg cgagcgcgta
    20701 ctgccgatgc catcctgcgt ttgatgggcg ggaaacgtca ggccaatgtt cagcacgatg
    20761 ccgataaccc gatgccgatc ccggaaggtg catttgccga agaagaacgt tacatgatta
    20821 acggcgtact gacgcttgcg tcgcgttctc tgcgcgggat catgacgccg cgcggtgaaa
    20881 taagctgggt tgacgctaat ctcggggtcg atgaaatccg cgagcaactg ctctcttcac
    20941 cgcacagtct gttcccggta tgtcgcggtg aactggatga aatcatcggt attgtacgtg
    21001 ctaaagaact gctggtggcg ctggaagagg gcgttgatgt ggcggcgatt gcttcggcgt
    21061 ctccggcgat tatcgtcccg gaaaccctcg atccgatcaa cctgttgggc gtgctgcgtc
    21121 gtgctcgcgg gagctttgtt atcgtgacca acgagtttgg tgtggtacaa ggtctggtca
    21181 cgccgctgga tgtgctggaa gccattgcgg gtgaattccc ggacgctgac gaaacgccgg
    21241 aaatcattac tgatggtgac ggctggctgg taaaaggcgg tacagatttg catgccttgc
    21301 agcaggcgct tgatgttgag caccttgccg atgacgatga tatcgcgacg gtcgcgggcc
    21361 tcgtgatctc ggcaaatggt cacattcccc gtgtgggcga tgtgattgat gtagggccac
    21421 tgcatatcac catcattgaa gccaatgatt atcgtgttga tctggttcgc attgttaaag
    21481 agcaaccggc gcacgatgaa gatgagtaag cattaacgta acggcataat gggcgtgata
    21541 tgtccattat gccgggcggg cggcggttgg ctgcccgcca gccatttggg aaaatcacgt
    21601 agcggcatcg gctgtgcgta caaataccct tgcaaaatat gcacgccatg acggcgcaaa
    21661 tacttcgcct gttcttgcgt ttccacgccc tccgccacca gttcaatatt cagtctttgc
    21721 cccagcgcga tgatgatatc ggtcaccgtc gagttaaccg cgtcagaacc tatagctgcg
    21781 gtaaatgact tatcaatttt cagcatgtca ggacgtaatg tttcaagcca ggaaaatgaa
    21841 ctgttgccgg tgccgaagtc atcaatcgcc agtttgacgt ttttacgatg tagctcgcgg
    21901 gcaatccgat aatcaacatc cagtaaggca tcgcgttcgg tgatttcgag gatcagttgc
    21961 tgaatcgggt gagcgctaaa ccagtactga ttgagatctt ttatcagcac accacggcga
    22021 aaatggctgg gtgcgacgtt aatgccaaca tgaaactgac tactcatcgg gaaaacatgg
    22081 cgctgacgaa tggtttctgc catcacatag cgggtcagtg gcacaattaa atgatgttct
    22141 tccgcgatag gaataaacac atccggtgaa atccagccct gacgcggatt gttccagcgc
    22201 agcaaaatct ctacaccaat acattgctgg ctgcgcgcat taagcaaagg ctgacagaac
    22261 aattcgaact catgttgcgc caggcccaga ttgatttcgc gggaaaagct catccggtaa
    22321 gcggtcgcca gccaggcgat gtagcccacc agtaaactta gcagcaccgc cagcggtaat
    22381 tgtgttggaa gatagtgcca tgccagcgcc gtagcccccg gaccattaac gttaatggta
    22441 aaaggaaagc gttgcgaaga aacctgatag ttttcattgt cttcaggttg cggaagggaa
    22501 tctaccagcc cattaccata gagcaaatgc cgtttgtcca ccgtcaggct ggcgctactg
    22561 atttgcggca gttgtggctc aaggagcatt gccgtcagta agtcgatgtt aatcatctcc
    22621 atgaccccag cattgctact gcccgctgca ggcgtccatt gaatcaaaac cggactgcct
    22681 ttaatcaggg cacggtcgat cgtcaggcgt aaaagtggtt gcggtgcagg aagttcagcc
    22741 agaatgtcca cgacgggcac attgcgataa ccaaaaatgc tggagcaata gagtgttccg
    22801 tcttgcacca ggccaatgga tcgcacagtt tggagttttg ccgcctgttt acgtaatggc
    22861 aaatgggcga cagagcaggg cagaccaatc agcggaagca agacttcgct acctgcctgt
    22921 aggggaagca gtactttatc taattcctct acagcgtgat tggcgaattg tactacccgt
    22981 tgttgattta agttacgctg tgaaataaat cgcacgctca gggtcgaagc gagcgtaacg
    23041 agtgcgcaaa tcgtacaaac aatcattcga ttacggcgat aggttttaat gatccgttgt
    23101 gctttttgca tgcgggccac cctgtaagcg tatggcgaca aacgcctgac gcaacagtgg
    23161 aagtgtagtg gggaatatta cagcagacga gaaagcgggt ataaattcgc ccatccgttg
    23221 cagatgggcg agtaagaagt attagtcaca ctggactttg attgccagac caccgcgtga
    23281 ggtttcgcgg tatttggcgt tcatgtcctt accagtttcg tacatcgttt cgatgacctt
    23341 atccagcgag acgcgcggtg cactggtgcg gcgcagagcc atccgtgcgg cgttaatcgc
    23401 cttcacagag gcaatggcat tacgctcaat gcacggcacc tgaacctgcc ctgcaaccgg
    23461 gtcgcaggtt aaaccaaggt tgtgttccat gccaatttcc gccgccacgc aaacctgttc
    23521 cgggctaccg cccagcagtt ctgcaagacc cgcagcagcc attgaacagg caacacccac
    23581 ttcgccctgg caaccaactt ccgcaccgga aatagaggcg ttcattttat acaatgcacc
    23641 aatcgcgccc gctgccataa agtaacgggt atagatgtcc gggctgaccg attcaataaa
    23701 gtggtcatag taagccagca ctgccggaac gataccgcag gcaccgttgg ttggcgcagt
    23761 taccacacga ccaccggcgg cgttttcttc gttaactgcc agcgcaaaca tgtttaccca
    23821 gtcaatgaca ttcatcggat cgttagacag tttatcgctg gaaactaaca tccggcgcag
    23881 ggcagacgca cgacgtggca cgcgcagcgg gcctggcagt acaccttcgg tgttcatccc
    23941 gcgatcgata catgcctgca tggtttgcca gacatgcgcg aaatactcgt cgatctcttt
    24001 cttgctgtgc agcgccagtt cgttctgcat agcgagacca gacagcgaat agccggtttc
    24061 attacagtac gcgagcagtt cggtggcaga tttgaacgga tacggcacgc tcacttcgtt
    24121 ggcagcatcc tgaccaaagt gttcttcatc gacgataaaa ccgccgccga tggaataata
    24181 agttttgctg tagacgactt catcgccgtt ataggcgtgg atttgcatac cgttttcatg
    24241 cagcggcagg ttgccgttat gaaaacgcat cccgttgtcg cgcgggaaat ccacttcatg
    24301 ccgtccctgt gccagcagca gacgttcgcg ctcttctacg tcgcgaataa aaccgggaat
    24361 actgtcgata tccacggtgg caggttcgtt acctgcaaga cccataataa tggcgatatc
    24421 ggtgtggtgg cctttacccg tcagcgacag tgaaccataa acgtccacgg caacgcgagt
    24481 aacgctatcc agtaagcctt tttcgaccag atcatcgacg aactgtttac ctgccttcat
    24541 aggccctacg gtatgggaag atgagggacc aatccccacc ttaaacatgt cgaatagact
    24601 aatcacgata atactcctga caaggtgact ggacttccag taacgaacta ataacagcgc
    24661 atagtgtaag agggaacgcg gcgactggct taactattca catgaattaa actaatcaaa
    24721 aaccgcgatt taactaatat tgcgatgatt gtctcaagta gtgttttgta gatgtaaaga
    24781 taagtatagt cagggtttca caccaatttg cagcgccagc tcacgaatta tgcctgcggt
    24841 cattccccat acaaaatact gttcgtacca ggacagccat acccgatgtg aatcgccacg
    24901 gcggtagata tctaaagggt gataacgacc cagatgtaat gcctgggcga gcggcatttc
    24961 aaacaccgcc gagacttcat cttcactggc gcgatacggc agatcgggcg ggataatgcc
    25021 gaccactggg gttacctgat agccagttac gctatcgacg ggcggcagca cgccgataac
    25081 ttcaacggcg gaaggcggta tagcgacctc ttcttcagct tcgcgcagcg cggcggcgat
    25141 aactgatgcg tccgtgtcat cgactgcacc tccagggaat gccacttgtc cagcgtgttt
    25201 acgcagatga attgaacgct gagtcagcaa caaccccggt tgcggtcgac ggacgatggg
    25261 gattaacacg gcagcctgac gatgatttag ggtttcccgg ttaatttgcg ggcgcaaaag
    25321 ttgaaagcgc gataaaaaat catcaagcat caggctacgg tattccacgt cttacttctc
    25381 cagttggcgt aatatcttat taactttatc aaaagtttcc tgatattccg cttcttcctg
    25441 gctatcggcg acaattccac cgcccgcaga gcagtatatt tgtccgttaa tggcagtcag
    25501 agtgcggata gtaatgctgg tatccatgtt gccgcaaaag ctcaaatagc caatgctgcc
    25561 gcaccaggca ttacgtcgct gcggttccag ttcgtcgata atttccatag cccgtacttt
    25621 cggagccccg gttattgagc caccaggaaa agctgcgcgc agcagatcgc tggcgtgtaa
    25681 ctgttctggt agttgtgccg ttatggtgct gaccagatga tgcacggcag ggaagggttc
    25741 caccacgaac agctctggta cttttaccga acctgctacg gcaacacgac cgatatcatt
    25801 acgcattaaa tcgacaatca tcagattttc ggcacgatct ttcgctgagt tcgccagttt
    25861 ttctgcttgt ttgctatctt cctgaggatc gggcaggcgt ggtagcgtgc ctttaatagg
    25921 gcgggtctgg atttcactat tatcacaaag aataaaccgc tctggcgaaa ggcttaaaat
    25981 tgcaccctgt tcaagacgta aaaaagcgct aaatggcgcg cggttggcct gattaagctg
    26041 aaggaatgcc tgccattcat cgccagaata ggtcgcatga aaacgctggg cgagatttac
    26101 ctgatagcaa tcaccgctgt gcagatattc ctgtacctgg cgaaattttt cgccgtactg
    26161 ctcgcgggtc atattggatt gccagtcgga agtaagcgtg aaatcttcct gcggcgagaa
    26221 ttgctggctt tccagccagg cccgacgggc attgacatca ttatgactca gcaaagaaac
    26281 tgtatgacgc tggtggtcga caatgagcgc ccaatcgtag atacccactg ccatatccgg
    26341 cagaacgata tcttgttccg caatttctgg cagtgactca aaacggcggc ccagatcgta
    26401 gccaaacaac cccagtgcgc cgccctgaaa tggcaaatct tcgttatgcg ttgggcgaat
    26461 gtctgcgcga tccagcacct gctggagcac ctgtagcggg tcatcagtgg tcgttgtgcg
    26521 tttttcgctt tcactaacaa cggtttcttt acctaaagtg gttaaagtgc aaatcggatc
    26581 ggcgaccaca atatcaaagc ggctatacgg atgatcggca tagccggagt gtaaaagcat
    26641 cgcccacggc aggtggctta agcgggagaa ataaaattca gcggcgtcct gacgccaggg
    26701 taaagtaatc acagcgggag ataacgtctt catcagtcct gactctactg gctatgtgcc
    26761 gggtagcatg aaataattag cgccaacaat ttagcaggag ttaacaatgt ttgcaggttt
    26821 accttcactc acccatgaac agcagcaaaa agctgtcgag cggatccagg aactgatggc
    26881 acaagggatg agcagcggcc aggcaatcgc gttggtggca gaagagctgc gtgccaacca
    26941 ttccggcgag cgaatcgtgg cgcgttttga ggatgaagac gagtaacaca ctaatccgta
    27001 ttgatttcgt tatacaagcc gatgattatt tgattgtgag aaaaaggacg ctttttatat
    27061 gtgattaaaa gcgtcctgat ccatttatgc tgatgtaaat tttactcaga ccagtgcttt
    27121 tagttcttcc tgcatatatg ttgaaaagta ttcaggattt ttaatcacag agcgttcacc
    27181 tgactccatt ttttttgccc atccaaccat tttttccgta aacccagggt tccagccttc
    27241 ctggtcagct cgcgccgtaa tatcagcggc actcgcaggt actcccagtt cttttaaata
    27301 tttaaagatt cccttcgccg tgctttcatt catcgaactt ggtgttggcg ctgatacatc
    27361 catggcacca atgaaatcca gggctttatc tattacagca ggcatattct tatccttaaa
    27421 attaaccatg ttatcgataa ggctatatat gacctta
//
LOCUS       DAJTXQ010000047        26814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000047 DAJTXQ010000000
VERSION     DAJTXQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.047"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..921
                     /locus_tag="OO515_001916"
     CDS             <1..921
                     /locus_tag="OO515_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575857.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="OO515_001917"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="OO515_001917"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6575858.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="OO515_001918"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="OO515_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6575859.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="OO515_001919"
     CDS             3882..4277
                     /locus_tag="OO515_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6575860.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="OO515_001920"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="OO515_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6575861.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="yraP"
                     /locus_tag="OO515_001921"
     CDS             4897..5472
                     /gene="yraP"
                     /locus_tag="OO515_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6575862.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="OO515_001922"
     CDS             complement(5586..6626)
                     /locus_tag="OO515_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6575863.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="OO515_001923"
     CDS             complement(6699..7334)
                     /locus_tag="OO515_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6575864.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="OO515_001924"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="OO515_001924"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6575865.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="OO515_001925"
     CDS             complement(7960..8403)
                     /locus_tag="OO515_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6575866.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="OO515_001926"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="OO515_001926"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6575867.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="OO515_001927"
     CDS             complement(8743..9246)
                     /locus_tag="OO515_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6575868.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="OO515_001928"
     CDS             complement(9240..9764)
                     /locus_tag="OO515_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6575869.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="OO515_001929"
     CDS             9973..10968
                     /locus_tag="OO515_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6575870.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="OO515_001930"
     CDS             10977..11855
                     /locus_tag="OO515_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6575871.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="OO515_001931"
     CDS             12061..13068
                     /locus_tag="OO515_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6575872.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="OO515_001932"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="OO515_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6575873.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="OO515_001933"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="OO515_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6575874.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="OO515_001934"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="OO515_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6575875.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="OO515_001935"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="OO515_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6575876.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="OO515_001936"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="OO515_001936"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6575877.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="OO515_001937"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="OO515_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6575878.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="OO515_001938"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="OO515_001938"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6575879.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="OO515_001939"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="OO515_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6575880.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="OO515_001940"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="OO515_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6575881.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="OO515_001941"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="OO515_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6575882.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="OO515_001942"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="OO515_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6575883.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6350 a         7446 c         6743 g         6275 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gact
//
LOCUS       DAJTXQ010000048        26750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000048 DAJTXQ010000000
VERSION     DAJTXQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.048"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            100..372
                     /locus_tag="OO515_001943"
     CDS             100..372
                     /locus_tag="OO515_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6575884.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            465..548
                     /locus_tag="OO515_001944"
     CDS             465..548
                     /locus_tag="OO515_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575885.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            648..1469
                     /locus_tag="OO515_001945"
     CDS             648..1469
                     /locus_tag="OO515_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6575886.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1508..1837)
                     /gene="mdtI"
                     /locus_tag="OO515_001946"
     CDS             complement(1508..1837)
                     /gene="mdtI"
                     /locus_tag="OO515_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6575887.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1824..2189)
                     /gene="mdtJ"
                     /locus_tag="OO515_001947"
     CDS             complement(1824..2189)
                     /gene="mdtJ"
                     /locus_tag="OO515_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6575888.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2601..3635
                     /gene="tqsA"
                     /locus_tag="OO515_001948"
     CDS             2601..3635
                     /gene="tqsA"
                     /locus_tag="OO515_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6575889.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3660..5048)
                     /gene="pntB"
                     /locus_tag="OO515_001949"
     CDS             complement(3660..5048)
                     /gene="pntB"
                     /locus_tag="OO515_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6575890.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5059..6591)
                     /gene="pntA"
                     /locus_tag="OO515_001950"
     CDS             complement(5059..6591)
                     /gene="pntA"
                     /locus_tag="OO515_001950"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6575891.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7115..8059
                     /locus_tag="OO515_001951"
     CDS             7115..8059
                     /locus_tag="OO515_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6575892.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8245..9627
                     /locus_tag="OO515_001952"
     CDS             8245..9627
                     /locus_tag="OO515_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6575893.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9664..10386
                     /gene="folM"
                     /locus_tag="OO515_001953"
     CDS             9664..10386
                     /gene="folM"
                     /locus_tag="OO515_001953"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6575894.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10383..10718)
                     /locus_tag="OO515_001954"
     CDS             complement(10383..10718)
                     /locus_tag="OO515_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6575895.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10847..11566
                     /gene="rstA"
                     /locus_tag="OO515_001955"
     CDS             10847..11566
                     /gene="rstA"
                     /locus_tag="OO515_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6575896.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11570..12871
                     /gene="rstB"
                     /locus_tag="OO515_001956"
     CDS             11570..12871
                     /gene="rstB"
                     /locus_tag="OO515_001956"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6575897.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12947..13876
                     /gene="tus"
                     /locus_tag="OO515_001957"
     CDS             12947..13876
                     /gene="tus"
                     /locus_tag="OO515_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6575898.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13873..15276)
                     /gene="fumC"
                     /locus_tag="OO515_001958"
     CDS             complement(13873..15276)
                     /gene="fumC"
                     /locus_tag="OO515_001958"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6575899.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15419..17065)
                     /gene="fumB"
                     /locus_tag="OO515_001959"
     CDS             complement(15419..17065)
                     /gene="fumB"
                     /locus_tag="OO515_001959"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6575900.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17264..18439
                     /gene="manA"
                     /locus_tag="OO515_001960"
     CDS             17264..18439
                     /gene="manA"
                     /locus_tag="OO515_001960"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6575901.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18540..20048
                     /locus_tag="OO515_001961"
     CDS             18540..20048
                     /locus_tag="OO515_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6575902.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20229..21539)
                     /gene="uidC"
                     /locus_tag="OO515_001962"
     CDS             complement(20229..21539)
                     /gene="uidC"
                     /locus_tag="OO515_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6575903.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21578..22951)
                     /gene="uidB"
                     /locus_tag="OO515_001963"
     CDS             complement(21578..22951)
                     /gene="uidB"
                     /locus_tag="OO515_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6575904.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22948..24759)
                     /gene="uidA"
                     /locus_tag="OO515_001964"
     CDS             complement(22948..24759)
                     /gene="uidA"
                     /locus_tag="OO515_001964"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6575905.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25148..25738)
                     /gene="uidR"
                     /locus_tag="OO515_001965"
     CDS             complement(25148..25738)
                     /gene="uidR"
                     /locus_tag="OO515_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6575906.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25959..26726)
                     /gene="hdhA"
                     /locus_tag="OO515_001966"
     CDS             complement(25959..26726)
                     /gene="hdhA"
                     /locus_tag="OO515_001966"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6575907.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6713 a         6930 c         6429 g         6678 t
ORIGIN      
        1 ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa ttgagggtat
       61 gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgccgcc
      121 tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgccg
      181 gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg
      241 gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca aaaagcgcag
      301 gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc tgctgcacaa
      361 cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg
      421 tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc
      481 gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc
      541 tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt
      601 tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtacaacca
      661 ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag
      721 acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg
      781 tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg gaaacggcca
      841 gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg gcaggacact
      901 gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt tttgtgtcaa
      961 ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat ccgacactgg
     1021 gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc gcgccgtggg
     1081 acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg ccgttatttg
     1141 agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa gtaactcagg
     1201 cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg
     1261 gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt gacagcggtt
     1321 cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa agttcggctc
     1381 ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg
     1441 acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg tgcgggcagc
     1501 gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc aggccaatcc
     1561 agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa
     1621 taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag
     1681 aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat attttgcgac
     1741 gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg
     1801 ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta
     1861 cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt ggttaacccg
     1921 gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat
     1981 aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt tttaacggcg
     2041 aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt
     2101 ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt agcaatagcc
     2161 aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat
     2221 tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata gggcaagctt
     2281 aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt
     2341 ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat tgtccataat
     2401 attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctcggtagtt
     2461 gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc acttctttac
     2521 ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta ttagcaaagg
     2581 aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc ctaaaaatcg
     2641 tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc gccgagatca
     2701 tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg gtgcaacaca
     2761 tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc atcatcgtga
     2821 tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg acgcggacgt
     2881 taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg ttgttgcaac
     2941 gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg aacgcggcga
     3001 tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca atatttttat
     3061 tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga aaatttcagc
     3121 aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt gacagtgttt
     3181 ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc gcctgggcga
     3241 tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc tttgcgctta
     3301 attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct caggtactgg
     3361 tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg attaatctgg
     3421 tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt tccacattgg
     3481 tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt atgctgattt
     3541 ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca
     3601 tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg ccgtcagggt
     3661 tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag catgtgggtg
     3721 ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat cgaacgttta
     3781 aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc aataggactc
     3841 ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac cagtacggta
     3901 tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc atacggtact
     3961 tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac cgggtggata
     4021 ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc gacaggatat
     4081 tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg ggagtttttc
     4141 agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc ctgatcatcg
     4201 ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa ggaacggttc
     4261 atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag cgcaccggtc
     4321 acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca gccggagtac
     4381 gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc gactaaatgc
     4441 cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg caggccgacg
     4501 ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc cgccaggttc
     4561 attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca cagtttgccg
     4621 aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag gaacacttcc
     4681 gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag atagctgtta
     4741 aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac cagttctggc
     4801 atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc aatgaccatc
     4861 gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc gattaacgca
     4921 atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg tttcgaaaga
     4981 ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt aactaatcct
     5041 ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct gagtcacggt
     5101 gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa ggaagctaac
     5161 ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt
     5221 ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca cgtaataacc
     5281 gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg gcgcaacgct
     5341 tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac gccacggtga
     5401 gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg cctgcggctg
     5461 agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga tcacggtcac
     5521 gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct ctttgcacaa
     5581 cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg tcggcagacg
     5641 gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga agatttcacc
     5701 cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa tcacactgcc
     5761 cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt tgcctggaat
     5821 aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga gttccatttc
     5881 cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccactgc ccgcttcctc
     5941 tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt tcacttccgg
     6001 acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa tggcggccag
     6061 acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc cggccgcagt
     6121 aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg cgcgataacc
     6181 ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac
     6241 agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta attccggatt
     6301 ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg caatttcatc
     6361 atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat tcccttctac
     6421 aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc
     6481 gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt ttggcgttgc
     6541 tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca tgatattccc
     6601 ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag ccgctcatgt
     6661 acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata acgtactgaa
     6721 aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg caaaatatta
     6781 acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga ctgataattg
     6841 tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact taacattaaa
     6901 ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg ttttttaagc
     6961 atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg aaaaatgacg
     7021 cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat aacaatacca
     7081 gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca ccctcctggc
     7141 gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac tgacaccgga
     7201 gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt ttaatgctat
     7261 tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg cctcttttta
     7321 tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg ctgacctcta
     7381 taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga
     7441 actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg tccagggctt
     7501 ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc
     7561 ctactctttc tacatcgttc gtcaaatcga tgccaaccaa ggcggcaacc agcgtattac
     7621 tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg atgtgatccc
     7681 ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt
     7741 tgagatccta ggtgttgcga ctaccgcatc accaagttct gaagtcggtc gtttctttga
     7801 aacccagtca tcaaaaggcg ggcgttacac cgtcatgctc ccggatggca ctaaagtcga
     7861 agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca aattctctgg
     7921 caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg
     7981 tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc tgaccgtcag
     8041 cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat gccgcctttc
     8101 gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc ttcgtaaaat
     8161 cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt attcttctta
     8221 tcacgtttta atcactggat atcgatgaaa aagaaactgg gactgagcgc actcaccgcg
     8281 ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca
     8341 gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg cattttattg
     8401 ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg cggtatcttt
     8461 acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg gggatactgg
     8521 ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc gttaagcttt
     8581 tttacggaca cgccggaatt gcgcctgttc ggcgatggta atacctggca atcgatcgtc
     8641 ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt gcaaaccgct
     8701 gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct gtttgttgtg
     8761 ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg acttgcactt
     8821 ggcgtacccg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg ggttttcatt
     8881 ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa
     8941 gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac gctgctttcg
     9001 ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat ggccggtctg
     9061 atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt gatcgtttcc
     9121 gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt cctggctgcc
     9181 actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc gccatccgcc
     9241 tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt
     9301 tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg
     9361 gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt
     9421 gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt gctgttatcc
     9481 gttgttctgt atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat
     9541 gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat tgcctcagtt
     9601 ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc aaaggagata
     9661 acgatgggta aagcccagcc cttgccaata ttaattactg gcggaggtcg tcgcatcggc
     9721 ctcgccctcg cctggcattt cattaatcaa aagcaaccgg tgattgtcag ctatcggacg
     9781 cactatccag ccattgatgg actgattaag gcaggtgcgc agtgtattca ggctgatttt
     9841 tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagccc ccatggtctg
     9901 cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc gccactggcc
     9961 gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct caaccatgcg
    10021 ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca ctttaccgat
    10081 tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa agcggcactg
    10141 gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt gaactctatt
    10201 gctccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca acaggcgctg
    10261 aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct ggtcgattac
    10321 ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg tcgtcatctg
    10381 cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc ccaacacgaa
    10441 acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca cagcgacacc
    10501 aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc gcgtaaggct
    10561 tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt
    10621 ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat caacagcact
    10681 accagcgcac cgagcgcggc tttgattacc agccccattt ttttacctta acacttccat
    10741 aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga tagtaagtaa
    10801 aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga acactatcgt
    10861 atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg caaaacatga
    10921 tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt tgcgagaaaa
    10981 tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga ccatttgtcg
    11041 tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg atagcgatat
    11101 gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa cgacgccccc
    11161 tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag ccacactgac
    11221 caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg gcacgttgac
    11281 catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc tctcgacagc
    11341 tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg accgcgatgc
    11401 attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg tggacgtggc
    11461 tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc gcattaaaac
    11521 tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga tgaaaaaact
    11581 gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc tgctggttgg
    11641 gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg atttgatgaa
    11701 cagttcactg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg actggggtaa
    11761 gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc cactgagtaa
    11821 ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg tcgccctgga
    11881 cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg cagttggtcc
    11941 tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc tgatcacttt
    12001 tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact ggcaggatat
    12061 gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg aacgtatcca
    12121 ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga tggcggacaa
    12181 tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc acgaactgcg
    12241 aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga gcgccgccga
    12301 atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag agctgctgac
    12361 ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag acctgccgtt
    12421 gtggctgtca acgcatctgg cagacattca ggcagtaacg cccgataaaa cggtacggat
    12481 taaaacgctc gcgcaaggcc attatgcggc gttggatatg cgcttaatgg agcgcgtgct
    12541 ggataatttg ctcaataacg ccctgcggta ctgccattca acggttgaaa ccagcctgct
    12601 actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga ttgccccaga
    12661 aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg atcgctcaac
    12721 cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa tgggcggtac
    12781 ggttaattgt gacaccagcg aactgggtgg tgctcgcttc tcgtttagct ggccgttatg
    12841 gcataacatc ccgcaattta cctctgcctg acactacgcg cacgatggtc aagtcaccac
    12901 cactgtgcta taaaataagt atgttgtaac taaagtggtt aatattatgg cgcgttacga
    12961 tctcgtagac cgactcaaca ctacctttcg ccagatggaa caagagctgg ctgcatttgc
    13021 cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc cggaggtaaa
    13081 aaaagaggat gagcataatc cgcttaatcg tattgaggta aaacaacatc tcggcaacga
    13141 cgcgcagtcg ctggcgttgc gtcatttccg ccatttattt attcaacaac agtccgaaaa
    13201 tcgcagcagc aaggccgctg tccgtctgcc tggcgtgttg tgttaccagg tcgataacct
    13261 ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga ccacgttcga
    13321 gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat gggtgcatcg
    13381 tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc tgcacgaccc
    13441 cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc atcgtgatga
    13501 agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac cgtggacgcg
    13561 cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc tgccacagaa
    13621 cgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg tctggtacaa
    13681 aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac tgattaatcg
    13741 ggataatggc gcgggcgtgc cggacgttgg tgagttgtta aattacgatg ccgacaatgt
    13801 gcagcaccgt tataaaccac aggcgcagcc gcttcgtttg atcattccac ggctgcacct
    13861 gtatgttgcg gattaacgcc cggctttcat actgccgacc atctgttctg gccgtaccca
    13921 gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt ttaaggtcag
    13981 cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa tgtgggtgtt
    14041 aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct cacgattcgg
    14101 ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca gcaagcgcac
    14161 cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa agttaccgga
    14221 agcgccaccc atgttgatcg ccacgtcgtt ccccattacc tgacagcaga gcatggttaa
    14281 tgcttcacac tgggttgggt tcactttccc cggcatgatt gagctgcccg gctcattttc
    14341 cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc ggacatcatt
    14401 ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct gaaccagggc
    14461 atcacaggtc gcaagcgctt caaatttgtt cggcgcggta acaaacggtg cacaggtaat
    14521 gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta gtccagtacc
    14581 caccgctgta ccgccgagag ccagttccgc tacgtgaggc aggctgtatt cgatatgttt
    14641 gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg ttagcggcgt
    14701 ggcgtcctgc aagtgggttc gaccgatttt gacgatatcg gcaaatgcac gcgatttttc
    14761 actcagtgtc tgtgtcaggg ttttaacctg cggaatgagt tgcttgcgca gcgccagcag
    14821 cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt tgttcacgtc
    14881 gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt cactggcccg
    14941 gttagccagc acttcgttca tgttcatgtt actttgcgtg ccggagccgg tctgccagat
    15001 agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg cctgccgaat
    15061 ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg ccgctgcgcg
    15121 cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg aaatgcggaa
    15181 atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga catcaatcgc
    15241 ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tgctcctcac ctgattaatt
    15301 ttttctttct gttttgcctt cgttaagcaa cttttagctc acttattatt tactatttga
    15361 taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc ggctctgttt
    15421 atttcacaca gcgagtgcac tgtgtgagtt gtatctgctg gaagaagtca tttcctttat
    15481 catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg gcttccattc
    15541 ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc aatacagcgg
    15601 ccggaccacc gatactgcca aggtagaagc cgccgtgttt tttacaggca tccgtcacct
    15661 gctggctgcg gttgcctttc gccagcatga tcatacttcc gccctgcgct tgcagttgat
    15721 cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag gcataacctt
    15781 ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac tgcggcagcc
    15841 cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga ccgacgataa
    15901 tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt gcgaggatct
    15961 ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct ttacgcagct
    16021 cttcagggat atatttacct ggattatgtt ccagtttttc gatccagatc ccctgacggt
    16081 tgatcttcgc tttgatatta cggtcagcag agcaggaaac acccatacca accggacagg
    16141 atgcgccgtg acgtggcagg cgaatcacgc gtatgtcgtg agcgaagtat ttaccaccaa
    16201 actgcgcacc cagaccgaga ttttgcgctt cgatcagcaa ttctttttcc agttccacat
    16261 cgcggaatgc ctgaccgtgc tcattccctt ccgttggcag ttcgtcatag tatttagcgg
    16321 aagccagttt cactgtttta aggttagttt ctgcagaagt tccaccaatt acgaacgcaa
    16381 tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc aggtaatttt
    16441 tcagtttccc cggcgttagt aacgctttgg tttcctgata gagatacgtc ttgtttgccg
    16501 aaccaccacc tttggcgata cagaggaatt tgtactcgtc gccatcaacg gcataaagat
    16561 cgatctgcgc tggcagattg gtgccggtat tcacctcttt atacatatcc agcggcgcgt
    16621 tttgcgagta gcgcaaatta tcttcgatat aagtgttata gacaccgcgc gccagcgccg
    16681 cttcatcaca gccaccggtc catacacgct gccctttttt accaacaata atcgcagtgc
    16741 cggtatcctg acaggttggc agaacacctt tcgccgcgat gtcggagttg cgcaggaatt
    16801 gcagcgccac atatttatca ttttcgctgg cttccgggtc acgcagaatg tcggccacct
    16861 gttgttggtg agccggacgc agcataaatg acgcatcatg aaacgcctga cgcgccaaca
    16921 gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca gatacgctaa
    16981 cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga aaaggagcct
    17041 gataatgaaa gggtttgttt gacattgttc tctcacttac tgcctggttt ggttatgctc
    17101 tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac acttttttaa
    17161 caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta ggattcttgc
    17221 tttaatagtg ggattaattt ccacattaaa acagggattg atcatgcaaa aactcattaa
    17281 ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt atggtatgga
    17341 aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga aaagcagttc
    17401 acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg agagtgataa
    17461 atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt tcctgttcaa
    17521 agtattatgc gcagcacagc cgctctccat tcaggttcat ccaaacaaac gcaattctga
    17581 aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg agcgtaacta
    17641 taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc ttgcgatgaa
    17701 cgcgtttcgt gagttttccg agattgtctc cctactccag ccagtcgcag gtgcacatcc
    17761 ggcaattgct cactttttac aacagcctaa tgccgaacgt ttaagcgaac tgttcgccag
    17821 cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa aatcggcgct
    17881 cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat tttacccgga
    17941 agacagtggc ctgttctccc cgctgttact gaatgtggtg aaattgaatc ctggcgaagc
    18001 gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc tggaagtgat
    18061 ggcgaactcc gataacgtgc tgcgtgcggg tctgacgcct aagtacattg atattccgga
    18121 actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga cccagccggt
    18181 gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct tctcgctgca
    18241 tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt tgttctgcgt
    18301 cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac cgggtgaatc
    18361 agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc gtttagcgcg
    18421 tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct aagcttatta
    18481 aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg ataatcatta
    18541 tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc tggacaggcg
    18601 gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc gcgcaggcga
    18661 acgcgcaact caaactgaca gctcctgaat ccaacctgga agtgagttat caaaactatc
    18721 atcgcggcgt attcagcagc cagttgcaac tgttggtgaa acccattgcc gggaaagaaa
    18781 atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat catggtccct
    18841 tcccgcttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca attcaaacca
    18901 cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt gaaacgcctt
    18961 ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt tcgctcaagc
    19021 cactgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag ttccagttaa
    19081 atgctgacag agacggcaaa gccatctccc tttccgggga ggcgcaaagt ggtcggatag
    19141 acgcagttaa cgaatacaac cagaaagtgc agttgacctt taataatctg aaaaccgacg
    19201 gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg tcactggaaa
    19261 aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg gagatcagcg
    19321 gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat tactcgctaa
    19381 acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta aaagtcggcc
    19441 agattgatgg tgaagcctgg catcagttta gccagcaata taacgcgcaa actcaggcgc
    19501 tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa gtgacggaag
    19561 ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact atcgcgccgc
    19621 taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc ctgaaagatc
    19681 cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt tcggttaaat
    19741 ctctggatgc gaaactgacc attccggtgg atatggcaac tgagtttatg actcaggtag
    19801 cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag caagttgaag
    19861 gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat accatcacca
    19921 ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg ccactggaag
    19981 atttcgttgg tatgtttgcg atgccagctc ttaacgttcc ggctgtaccg gcaattccgc
    20041 agcagtaatt cacggccaaa acgccggaga attttcactt cggcgttttc atctgaaaac
    20101 aaaccagaga ttgcctgatg cgctacgctt atcaggccta ccttttcctg caacactttg
    20161 aatttatgag tttttgtagg ctggataagg cgtttacacc gcatccggca taaaaaacgc
    20221 gcactttgtc aacaatctga aacgccggag gtttttctct ccggcgttat ttctgacttc
    20281 agcataaagt catactgaat actcattttc acatcccagt aattcaggcg atcgcccagc
    20341 gtactgccat cgcgcttttc ccgcccttta tcggcagcaa gaaagaaatt catcccttgt
    20401 aacggccctt cgctgacccg atagttcata ccacctaaga tacccgcgcc ttcaatccgc
    20461 acatctcgtt tactggattc atagccgcga gtcacaatct gtgcaaatgt cagtgtaaat
    20521 tgcggcgtta aacgatagtt gacgcccaat tgccaggact gcattccttc acggttacga
    20581 tcaagactta atgaaaaggg atagccaatg tcggtatcca ccacggcacc attcattgca
    20641 tggcgcaaat aaccaccact gccaaatacg cttccccacg gcgcattcca ggtcaattgt
    20701 ccactaacca acgccgtttc attgggctgg ctggtattac gacttaaacc ttcgagctgc
    20761 gcgtaaaagc cgagcaactc ccctttcaac gtataattcg ggtcgagcgg tatgcttttc
    20821 tcgagaatac tttgtatctg attggaaaca tctttctgta ccgatgcccc cagagaatag
    20881 cgactggtat catcatgcca actggcggct aaaaaagaac gtgcgcgatg tttatcgtat
    20941 tttcccgtct ggctgttata caccccttca tcacgaatac cggttgttga atcagtccat
    21001 aaaaatactc gtgaacgcca cattccccac agatcaatat ttggtgttaa agcccaataa
    21061 cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg aactgccagc cattaacgga
    21121 atacgataag gtaagctacc ataactataa tccgtcccga agcgacctga tttaagcagg
    21181 ctattttcac tgggttttat ttttactgcc cccagggcaa agccaaaact tttatcacca
    21241 tcaagatacc accgggtact catatcagca cgagcaccta atttataaac ataatatgcc
    21301 ccgccttcaa cgccaataat attggaataa taaccactat tgaaatcgag caatcctccc
    21361 tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg gcttatccgc cctgcgcaat
    21421 tcgtttttca gtctgatgcg tagtcccgcc tcatcgtcag ccagttgcgc cgcataagca
    21481 gagataaggc cagaggcagc cgtcaggcaa ataacggcca tggccactat ttttctcata
    21541 gttgcacctt tgatgttctg attattttta ttgattatta attagtgata tcgctgatta
    21601 attgctgctg cactttttta cgattatcaa tttcaaccac gatttctttg aattttttat
    21661 ccgtaagcgg ataaaaccag ataataacga atgccagtag cataaatccg caaggtacta
    21721 aggcaattga tgtgcggatg cccataataa cttccggcgt ttgcacctga ttggcaatat
    21781 atccgcttaa ccccaaaata aaggcaggaa ttgaacctcc gattgcctga ccgcatttac
    21841 gggtaaatga gaatagtgaa taggtgagcc cttcaattcg cacgccggtc aggtattcac
    21901 cgtattctac ggtatcagct tccagtgccc acatcactgt catagtaacg ccctgaccaa
    21961 ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagacggaa acccagaaga
    22021 atagcagata accgcaggtt cccagcaaag cgccaatcag gaaggtattc tttttaccga
    22081 tcctcgcgac catccccggc acaagcggtg ccgatgccac agtaccaacc aggttttgca
    22141 ccagtaccag cacagtgaac agcccggtat catttaacac atagcgcacg tagaacaacg
    22201 acgaggcgct gaccgcaaag gtcgaaatca gcacacacag cgcaccgatg cacaacataa
    22261 acagcgggcg attccgtttc agggtttgca aactgatctt caatgacggc tgcgcaacga
    22321 tacgtaccac attctcacgc gtcgatttga agcagatgaa gtaaagcacc attccggcaa
    22381 tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg ctggagttct
    22441 taatgctcgg ccctatcaga aatgccaggc agacaaaggt caatgaagcg gcaatcccac
    22501 gagccgcgcc cagacgggcg cgggattgtg gttgttgggt catcgcggta gcaagtgaac
    22561 cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc aaatatgcat
    22621 acactacttt gcggctatgg ctccagtcgg tcggtaccca gaataccagc acgctgaaga
    22681 tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa ttttccccag cgggtattca
    22741 cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc cgcaccagta
    22801 acagcatggt gcccgccgca gcggcaccga cgccagcgac gtcggtgtag taactcaaca
    22861 ggaagagcac ccccattgcg aaggcgaagt tattggcgac gtcaccgagg ctgtagccga
    22921 cgatggtgcg ccaggagagt tgttgattca ttgtttgcct ccctgctgcg gtttttcacc
    22981 gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg gtttgcggtc
    23041 gcgggtgaag atccccttct tgttaccgcc aacgcgcaat atgccttgcg aggtcgcaaa
    23101 atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa agacgcggtg
    23161 atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt acattgagtg
    23221 cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat gcagtttctc
    23281 ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat cgccgctttg
    23341 gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc tgatggtatc
    23401 ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg ggtcgagttt
    23461 acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac gggtatccgg
    23521 ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg ctatcagctc
    23581 tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt cgctgtacag
    23641 ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa agccgacagc
    23701 agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca tctcttcagc
    23761 gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta atgcgtggtc
    23821 gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt catgacgacc
    23881 aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct tcactgccac
    23941 tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt tggctgtgac
    24001 gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca ccacttgcaa
    24061 agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac gcagttcaac
    24121 gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac agtcttgcgc
    24181 gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt agagcattac
    24241 gctgcgatgg atcccggcat agttaaagaa atcatggaag taagactgct ttttcttgcc
    24301 gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt cgttgttcac
    24361 acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat cggcttcaaa
    24421 tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca ctttgccgta
    24481 atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt tcggtataaa
    24541 gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat cggcgaactg
    24601 atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc tttcccacca
    24661 acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc cgtcgagttt
    24721 tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct cattaagata
    24781 aaaatactgg tcaaccttta atctgattag ttaaccaatt ctgtgttgca cacagttatc
    24841 tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg atcaaaaaac
    24901 caaaaactgc aatacccaca gcgtgatatt gatcgcatta atctttaaaa cggcgttgac
    24961 agaagccata aaataagcaa ccgaacgttc gttcgagcat attgggaacg ataaactctc
    25021 atgacatatg gctacggtga atatttttgg gggggaattc tccgatcccg gctagattgt
    25081 attttgtaca gggaaattaa agtatgaaac gcgggcacag ttatcaccag cccgcgaaat
    25141 gagaggatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc tgagcaaacg
    25201 acgcttcacg cgccagatct tccgcttcga tatcggccag tacaccgtag gtcagatcca
    25261 gcagtaaacg tgaagtcatt gacgtgttga tgtctgggct tatctcgcct ttttgctggg
    25321 catcacgcat ccgctgggca acaaattccg tgatcgtcat atgcttattt ttaagcatgg
    25381 cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc gcccgttgcc
    25441 caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca aaatgaatcc
    25501 ctttaatcgg ttcccggaaa cgagccagcg ccctctcctg gtcctgtaag ataatcgcct
    25561 gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta atggcgcaag
    25621 atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa atctctctgg
    25681 cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta tccatcatta
    25741 aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc gcctatcatt
    25801 gctgttgaat agtgcgaagg cacactctat tcgtatactt ttcactctga aattggttaa
    25861 ccaatagccg tgatttggct gccagttatt tagcagagac gttgatgagt ttatcccggt
    25921 aaacaacacg cacggttgtt taccggtccg ttagtgtatt aattgagctc ctgtacccca
    25981 ccaccggaga cggtgagaat ttgtccgctt acccagctcg cagcaggcga gcaaaggaac
    26041 agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt gtgctgtaac
    26101 attttttgtt caatttctgg tgtaataacg gatttcaggg catcggttaa tattgccccc
    26161 ggcgcaatgc catttacccg aatatttttt tcacccaggt caaacgccat atttctgacc
    26221 agatgactgg ctgcagcttt agatgatgca taggaagtca tgtttatatt tttattttct
    26281 gccgccatag aagtgatggt cagaataacg ccaccgccat ttttttccat ttctggcgca
    26341 acaagttgtg acagatggaa aaaagaaaac acattcagtt cataagcacg gcgaaaatcc
    26401 gccattggca tatcaaacgg tttaggtcca ccgccaccgg cgttgttaac cagaatatca
    26461 actttaccca gcttactgat agcaaagtct gccagtgcag agagttcctg ttcggaagta
    26521 atatcacaac ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc tacaacatgg
    26581 ttagctgcgt cggcgttaat atcactgacc accacagatg cgccagctgt cgcgaatgta
    26641 atggcgattt ctttaccaat acctgcaccc gcacctgtga tgatggcgca tggcgcatgg
    26701 cgcatggcgc atggcgcatg gcgcatggcg catggcgcat tttccgtcga
//
LOCUS       DAJTXQ010000049        25778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000049 DAJTXQ010000000
VERSION     DAJTXQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.049"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..831)
                     /gene="dgt"
                     /locus_tag="OO515_001967"
     CDS             complement(<1..831)
                     /gene="dgt"
                     /locus_tag="OO515_001967"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6575908.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
     gene            915..1613
                     /gene="mtnN"
                     /locus_tag="OO515_001968"
     CDS             915..1613
                     /gene="mtnN"
                     /locus_tag="OO515_001968"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6575909.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1606..2406
                     /gene="btuF"
                     /locus_tag="OO515_001969"
     CDS             1606..2406
                     /gene="btuF"
                     /locus_tag="OO515_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6575910.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2444..3067
                     /locus_tag="OO515_001970"
     CDS             2444..3067
                     /locus_tag="OO515_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6575911.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3114..3458)
                     /gene="erpA"
                     /locus_tag="OO515_001971"
     CDS             complement(3114..3458)
                     /gene="erpA"
                     /locus_tag="OO515_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6575912.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3451..3516)
                     /locus_tag="OO515_001972"
     CDS             complement(3451..3516)
                     /locus_tag="OO515_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575913.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3540..4961)
                     /gene="clcA"
                     /locus_tag="OO515_001973"
     CDS             complement(3540..4961)
                     /gene="clcA"
                     /locus_tag="OO515_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6575914.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5186..6466
                     /gene="hemL"
                     /locus_tag="OO515_001974"
     CDS             5186..6466
                     /gene="hemL"
                     /locus_tag="OO515_001974"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6575915.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6624..8606)
                     /gene="fhuB"
                     /locus_tag="OO515_001975"
     CDS             complement(6624..8606)
                     /gene="fhuB"
                     /locus_tag="OO515_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6575916.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8603..9493)
                     /gene="fhuD"
                     /locus_tag="OO515_001976"
     CDS             complement(8603..9493)
                     /gene="fhuD"
                     /locus_tag="OO515_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6575917.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9493..10290)
                     /gene="fhuC"
                     /locus_tag="OO515_001977"
     CDS             complement(9493..10290)
                     /gene="fhuC"
                     /locus_tag="OO515_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6575918.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10341..12530)
                     /gene="fhuA"
                     /locus_tag="OO515_001978"
     CDS             complement(10341..12530)
                     /gene="fhuA"
                     /locus_tag="OO515_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6575919.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12750..15284)
                     /gene="mrcB"
                     /locus_tag="OO515_001979"
     CDS             complement(12750..15284)
                     /gene="mrcB"
                     /locus_tag="OO515_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6575920.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15480..17909)
                     /gene="hrpB"
                     /locus_tag="OO515_001980"
     CDS             complement(15480..17909)
                     /gene="hrpB"
                     /locus_tag="OO515_001980"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6575921.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17983..18513
                     /gene="thpR"
                     /locus_tag="OO515_001981"
     CDS             17983..18513
                     /gene="thpR"
                     /locus_tag="OO515_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6575922.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18528..19232
                     /gene="sfsA"
                     /locus_tag="OO515_001982"
     CDS             18528..19232
                     /gene="sfsA"
                     /locus_tag="OO515_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6575923.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19410..19865
                     /gene="dksA"
                     /locus_tag="OO515_001983"
     CDS             19410..19865
                     /gene="dksA"
                     /locus_tag="OO515_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6575924.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19902..20828
                     /gene="gluQRS"
                     /locus_tag="OO515_001984"
     CDS             19902..20828
                     /gene="gluQRS"
                     /locus_tag="OO515_001984"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6575925.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20867..22285
                     /gene="pcnB"
                     /locus_tag="OO515_001985"
     CDS             20867..22285
                     /gene="pcnB"
                     /locus_tag="OO515_001985"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6575926.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22282..22761
                     /gene="folK"
                     /locus_tag="OO515_001986"
     CDS             22282..22761
                     /gene="folK"
                     /locus_tag="OO515_001986"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6575927.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23131..23715
                     /locus_tag="OO515_001987"
     CDS             23131..23715
                     /locus_tag="OO515_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6575928.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23820..24560
                     /locus_tag="OO515_001988"
     CDS             23820..24560
                     /locus_tag="OO515_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6575929.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24595..25778
                     /gene="htrE"
                     /locus_tag="OO515_001989"
                     /pseudo
     CDS             24595..25778
                     /gene="htrE"
                     /locus_tag="OO515_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6332 a         6984 c         6419 g         6043 t
ORIGIN      
        1 aaggtctgcc acacaatagg agatgtcgtc ggcagcttcc ataatccacg ttaatggaaa
       61 acgactgtaa agcgccaaat taagttcttt acgcaaccgg gcaatatagg cttcttcaga
      121 aagataataa cccggctttt tcattaaata gtgatgtgtc tcaggcgttt cgccacgcca
      181 ccacgccgga cgggtatatt ttaaaatacc gccaacctgt gcccaagtga gattcatccg
      241 catcaatgta tgcaccagac gaatgccttg tgcattcccc tcaaaatgac ataagtcctg
      301 acgaatcttg cgccgcagct cgttaagctg ttcttccccg tcccgtaaac gtagtgccgc
      361 cacgctgcag cgatcgtcag tcagaggctg gctttcggca tcttccgggt gcaaacgttg
      421 gcgaaaccag tcatttatcg ctgcttcgcc aaaatgacca aacggcggat tgccgatatc
      481 gtgcatcagg catgacatct caacaatgct ttcaaagggg ccggtcagtt catccaggcc
      541 gtatgcttcc agcaatttaa gctctttcag acggcttaaa atttctttgg cgatgtagcg
      601 tcccacctgc tggacttcca tcgagtgggt aagacgcgtg cgcacggcgg cattgcgctc
      661 cagtggaaaa acctgggtct tttgttgcag acgacgaatt gccggagagt tgatgatacg
      721 cccgcgatcg ctctcgaaga tccgcaggat ctcatgttcg gttttaacgc cctgcggtga
      781 acggtaacga cgatgccagt ttattttttt tcggaaatca atctgtgcca tgtcctctcc
      841 cgcgtgagaa atacgcttcc ccgtaagcgc atggtaaact atgctttcaa atcgggctta
      901 tcgcgagtaa atctatgaaa atcggcatca ttggtgcaat ggaagaagaa gttacgctgc
      961 tgcgtgacaa aatcgaaaac cgtcaaacta tcagtctcgg cggttgcgaa atctataccg
     1021 gccaactgaa tggaaccgag gttgcgcttc tgaaatcggg catcggtaaa gtcgctgcgg
     1081 cgctgggtgc cactctgctg ttggaacact gcaagccaga tgtgattatt aacaccggtt
     1141 ctgccggtgg cctggcacca acgctgaaag tgggcgatat cgttgtctcg gacgaagcgc
     1201 gttatcacga cgcggatgtc acggcatttg gttatgaata cggtcagtta ccaggctgtc
     1261 cggcaggctt taaagctgac gataaactga tcgctgccgc tgaggcctgc attgccgaac
     1321 tgaatcttaa cgctgtacgt ggcctgattg ttagcggcga cgctttcatc aacggttctg
     1381 ttggtctggc gaaaatccgc cacaacttcc cacaggccat tgctgtagag atggaagcga
     1441 cggcaatcgc ccatgtctgc cacaatttca acgtcccgtt tgttgtcgta cgcgccatct
     1501 ccgacgtggc cgatcaacag tctcatctta gcttcgatga gttcctggct gttgccgcta
     1561 aacagtccag cctgatggtt gagtcactgg tgcagaaact ggcacatggc taagtcactg
     1621 ttcagggcgc tggtcgccct gtcttttctt gcgccactgt ggctcaacgc cgcgccgcgc
     1681 gtcatcacgc tttctcccgc caacactgaa cttgcctttg ccgccgggat cacgccggtt
     1741 ggggtcagca gctattccga ctatcctcca caagcgcaaa agattgagca ggtttccacc
     1801 tggcagggga tgaatctgga acgcattgtc gcgctgaaac ccgatctggt gattgcctgg
     1861 cgtggaggta atgccgagcg gcaggttgac cagctggctt cgctgggaat aaaagtgatg
     1921 tgggtcgatg cgacaagcat tgaacaaatt gccaatgcgt tacgtcaatt ggccccctgg
     1981 agtccgcaac cggataaggc cgaacaagcc gcgcaatccc tgctggatca gtacgcgcaa
     2041 ttgaaagcgc aatatgctga taaacctaaa aaacgtgttt ttctgcaatt cggcattaat
     2101 ccgccattta ccagtggaaa agagtcgatt caaaaccagg tactcgaagt ttgtggcgga
     2161 gaaaacatct ttaaagacag ccgggttccc tggccgcaag ttagccgcga acaggtgtta
     2221 gcacgctcgc cacaggcgat tgtcattaca ggcggaccgg accaaattcc taaaatcaaa
     2281 caatactggg gtgaacagct caaaattccc gttattcctc tcacgagtga ctggtttgaa
     2341 cgtgcaagcc cacgtattat cctcgctgca caacagctct gtaatgcgct ttcacaggta
     2401 gattagcgcc tgaccttttt aggtgctcag tggggattga acaatgctcg tctattggct
     2461 ggatatagtc ggcacagcgg tatttgccat ctccggcgtt ttgttagccg gaaaattgcg
     2521 tatggaccct tttggtgttc tggtactggg cgtggttacc gctgtaggcg gcgggacaat
     2581 tcgcgacatg gcgctggatc acggcccggt attttgggtg aaagatccca ccgatctggt
     2641 cgttgcaatg gtaaccagca tgctgaccat cgtgctggtg cgccagccaa gacgcttacc
     2701 aaaatggatg ttgccggtgc tggacgccgt tggtctggcg gtgtttgtcg gcattggcgt
     2761 gaataaagcc tttaatgcgg aagtcggtcc gttaatcgcg gtttgtatgg tcgtcattac
     2821 tggcgttggc ggcgggatca ttcgtgatgt actggcccgc gagatcccca tgattttacg
     2881 tacagaaatc tacgcaactg cctgtattat cggcggtatt gtccacgcta cggcttatta
     2941 cacattttcc gtaccactgg aaacagccag tatgatgggc atggtcgtga cgctattgat
     3001 tcggctggcg gctattcgtt ggcatcttaa gctaccgacg tttgcgctgg atgagaatgg
     3061 gcgttgaaga gagaagatgc gccggatagt ttatccggca catgaacaac agattagatg
     3121 ctaaaggaag aaccgcaacc gcaggtgctt ttcgcgttcg ggttggtcac gatgaaacga
     3181 gaaccttcca gtccttcggt atagtcaacg gaaccgccga ccagatattg caggctcatc
     3241 ggatcaacca ccaagccaac gccctgtttt tcgatggtca tatcgccttc gttcacctga
     3301 tcatcaaagg tgaaaccata ctggaagccg ctgcaaccgc caccggtgat atacacgcgt
     3361 aatttcagat tcgggttatc ttcgtcagcg atcaggcttt taactttgtt ggctgctgcg
     3421 tcggtaaact ccagcggcag tgctacgtca tcactcatat tttgctccaa acgacatcgg
     3481 caattgggca aattctaacc caataataat cgccattatc taataccctg gtatttcgtt
     3541 caagtattct cgctggctga tgcggccttg cttcgcgcca gttgctcagc ttcctgtttt
     3601 gccagcgtgc gcgcaagaat cgccgagtat agcggtttcc cgccggtaaa ttgcgctaat
     3661 agtgttgcgc caagaccggt aataatcatt ggcaaaatga gctggtagtt atcggtcatc
     3721 tccagaacca gaatgatccc cgttaacggc gcgcgaatag atgccgccag taatgccccc
     3781 attccggcaa tagcaaacgt ccccgcctca aggtgatatt gcggaaacag ctcaacggca
     3841 accattccga aagcggttcc cagcacagta cccagcgcca gcatcggggc aaaaataccg
     3901 cccggcgcgc cggaagagaa gcagagtaag gtggtaatga cccgagcgac gaagataaac
     3961 accagcattc ccatgctgaa attccccgcg gtagcgatag gaatcaggtt aaaaccgccg
     4021 cccgacgttg ctggtgccac aaaccccagc aatccacaca gaccgccaat cgcaccgccc
     4081 atcagtaccc atttggtaat attgccgccg tgcacacggt gcagcaaatc ctgcatcccc
     4141 agcacccatt tattaaaaat agggccgaaa atgccaaaaa taataccgag gatcagataa
     4201 agccacagcg tattaagcgg cgcatcagaa agtttaccga cgtcaatcaa cgcaacttca
     4261 tgattaaaaa tccggtacat aatggtcgac ataatgacgc caataaatac cgcttttatc
     4321 gaaattaacg tatagcgaaa ctgcggacgc atctcttcga taataaacaa aatacccgcc
     4381 agcggcgcgt taaaggccgc agccagcccc gcagcagcac cggttgccag cagcgtatgg
     4441 cgagcttcgt cacctttcag gcggaaaata tcaagcacca tacggccaat gttaccgccg
     4501 atctgcacgg ttggcccttc gcgccccaac accatgcctc cgccgagtgt ccccagcccg
     4561 ccaaagaact tcaccggcaa tacacgccac cagcgaacgg gacgttgatc ttccagcgcc
     4621 ccttcaattt ccgggatccc cgaaccacct gcttccggcg cgtatttgcg caccaaaaag
     4681 tagccaaaca tcgccagcac cgccgaacag agaaaagcga cggttaacag aagcggataa
     4741 ttatcagcag tatgtaccag cgcccccata cgttggttct gcaaccaggc gacacctttg
     4801 tcaaaagcaa ccgctgccag cccaacaagc gtgccgacga ctgccgccat aaacaaaatg
     4861 gctaacgggg ttttatcgcg ctcaagaagt tggcgaatca gttgtctgcg tcgcaggcgc
     4921 gcggcctgcg gtgtttctaa agagggagta tcagttttca tccaatgatc acttattggt
     4981 catacaaata agatgacggc attttactcg cacgttccat gaaaatcctt tgcaaagcgt
     5041 aatgtttcaa atgcacaaaa cttttatact ctaagaaatc aggattgcac cagtacaagc
     5101 agcctgatgt ttgacgagta tttaacttgt tatgaataac atagaatagc agccattcac
     5161 tttttctacg aatcaggaac cctccatgag taagtctgaa aatctttaca gcgcagcgcg
     5221 cgagctgatc cctggcggtg tgaactcccc tgttcgcgcc tttactggcg tgggcggcac
     5281 tccactgttt atcgaaaaag cggacggcgc ttatctgtac gatgttgatg gcaaagccta
     5341 tatcgattat gtcggttcct gggggccgat ggtgctgggc cataaccatc cggcaatccg
     5401 caatgccgtg attgaagccg ccgagcgtgg tttaagcttt ggtgcaccaa ccgaaatgga
     5461 agtgaaaatg gcgcaactgg tgaccgaatt ggtcccgacc atggatatgg tgcgcatggt
     5521 gaactccggc accgaggcga cgatgagcgc catccgtctg gcccgtggtt ttaccggtcg
     5581 cgacaaaatc attaaatttg aagggtgtta ccacggtcac gctgactgcc tgctggtgaa
     5641 agccggttct ggcgcactca cgttaggcca gccaaactcg ccgggcgttc cggcagattt
     5701 cgccaaacat accttaacct gtacttataa cgatctggct tctgtacgcg ccgcgtttga
     5761 gcaatacccg caagagattg cctgtattat catcgagccg gtggcaggca atatgaactg
     5821 tgttccaccg ctgccagact tcctgccagg tctgcgtgcg ctgtgcgacg aatttggcgc
     5881 attgctgatc atcgatgaag taatgaccgg cttccgcgtg gcactggctg gcgcacagga
     5941 ttattacggt gtggaaccgg atctcacctg cctgggcaaa atcatcggcg gtggaatgcc
     6001 ggtaggcgca ttcggtggtc gtcgtgatgt aatggatgcg ctggccccga cgggtccggt
     6061 ctatcaggcg ggtacgcttt ccggtaaccc aattgcgatg gcagccggtt tcgcctgtct
     6121 gaatgaagtc gcgcaaccgg gcgttcacga aacgttggat gagctgacaa cacgtctggc
     6181 agaaggtctg ctggaagcgg cagaagaagc cggaattccg ctggtcgtta accacgttgg
     6241 cggcatgttc ggtattttct ttaccgacgc cgagtccgtg acgtgctatc aggatgtgat
     6301 ggcctgtgac gtggaacgct ttaagcgttt cttccatatg atgctggacg aaggtgttta
     6361 cctggcaccg tcagcgtttg aagcgggctt tatgtccgtg gcgcacagca tggaagatat
     6421 caataacacc atcgatgctg cacgtcgggt gtttgcgaag ttgtgatcca gcatggtttg
     6481 tcggatgcgg cgtgaacgcc ttatccgacc tacgattcgg ggccgtttgt aggcccgata
     6541 agacgcgcca gcgtcgcatc gggcatccgt tgtcgcgtaa acgctttatc cgtccaggcg
     6601 tacagggccg ttatatggaa aaattaacgg ctctgctttc tcagcaaata gataaaatat
     6661 ggcgcgccga taaaggtcga cagcagcccc gccgggatct ggaatggaaa cagcaccatc
     6721 cgcccacacc agtcagcgaa taccagcagt aaaccaccca ccagcgccga aattacgatg
     6781 tgtggcatcg tccgtcgaaa gcccatcatc cgcgcaatat gcggtgccat taaaccaaca
     6841 aaactcaacg gtccaatagt catcgtcgcg gtcgccgtca ggcaagccgc taacagcagc
     6901 agcgcaattc gcgtcggcgt cagcgccatt cctacggctc gtgcggtatc accacccagc
     6961 ggtaaaatgg tcagccagcg gcggtacagc ggggtaatcg ccagcaaaat caccatcaca
     7021 attccggtac gccagacctg cgcatcggtc gcgttgtagg tcgaaccgga aatccaggtc
     7081 agcacttgcg ccattcgcgg gtcaccgctc gcctgcaaca tcatcaaaag catggtgaat
     7141 gcggtactta acgccatccc cgccagtaac atacggtgtg gggaaaatcc accgcggccg
     7201 gcggcgatca taatgatcaa cagcgtcacc gccgccccga tactgcccgc gggtaacagc
     7261 cagccaaagg cattacccgg caccagaaac agcattaaca ccacgccaaa cgccgcgcca
     7321 gagctaatcc ccagcacttc cgggcttgcc atcgggtttc cggtcagccg ctgaataata
     7381 cagcccgcca ccgccagcat gacgcccgca aacagcgccg ccataattcg cggccagcgc
     7441 cagggcatca aatcctcgag caacgccccg ctcgcccacg tccagccgtg cgcatcacga
     7501 ccaaacgaca gcgccaccac cacagccatc aacagcagca cgccgcccgc gagggcaaac
     7561 gccagcacat gttggcgttc agccgcgaca cgatcgttga ccttcatatc cggcgcgcta
     7621 atgctgcgta aacgcggcaa cagccacagt agcagcggcg caccgatcaa cgcagtgacc
     7681 gaaccggtgg acacttccat ccacacgcga gtcagccaga ggatgatttg atcggaaagc
     7741 cagaggatca gcgccccaat cagcgacgcc agcatcaacc gtggcaacaa acgccgcgcc
     7801 cccagcattt tcgccagcag cggcgcgaac aaaccgataa agccgataat ccccaccgca
     7861 ttcaccagca gcgcactgat gactatcgcc agcgacagcg ccgccagacg cgcgagcgac
     7921 aaggccagcc cgagattgcg cgccacgcca tcatcaagcc ccatcagggt taacgggcga
     7981 agtagcaata acgtcaacat cacgccgccc agcaactgcg gccataaacg ctcaacgccg
     8041 ccccagtcgg tttgcgtcag cgttccggtg ctccacagga acatgctttg cagctggtcg
     8101 tgatggaaga taaccagtag ctgattaatt gccccgcaat aaaggctcac caccagcccc
     8161 gcgagaataa gcgtaaccgg cgaaagccgt ttcccccacg cgacgccaaa gacgattaag
     8221 ccaacgacac aagcgcccac cagtgcggca aactggctcg ccatagcacc tgggatcgcc
     8281 caaagtgtgg tgacggtaat tcccagttgc gcgcctgtag caacgccaag cgtcgtcggc
     8341 tccgccagcg ggttacgcag cacttgctga aacagcacgc ccaccagccc caggcccgcg
     8401 cccaccagca gcgaaatcgc cagacgcggc aacaagctgt agtgaaaaat catctgctcg
     8461 atgacatcga tgtctggcga ccaggcagcc tgcgcccact ggctacgcgg cagcgcctgc
     8521 gagaagttca tccaggtgag cgccgtagcg acaatcacta acagcgccag caataacgcc
     8581 ggaaaaagcg caattcgttt actcacgctt tacctccgat ggcgttatcc agaacgcgca
     8641 caaagtgcat tgccgagagt gtcgcaccat aaaaccagac tgcaggtacg cgctgaaagc
     8701 gtccggcgcg gacaaacggc atggcctgcc acagcggggt tgccattagc gcatccatgt
     8761 ctttgctgtt gtcgtgatca aaacagagca catcaacgtc tttatacgct gccagacgat
     8821 cgatactgac ggcggtactg ccccagaagt tggtttcccc ttgccaggca tttgggatgc
     8881 cgtactcatc gagaatttcc tggaacaagc tgtttggacc gaagaccagc atatggcgcg
     8941 gatcgataag cgtcgtcagc agtaacgggc gtgcaccacg cttcacaaag cggggtttca
     9001 tgctgcggat aaagtcttcg tattgcgcta aatgcgtttc cgctgcgctt tgcaggttaa
     9061 gtaaatctgc catttccgtc agcgatttac gcgccatcgc caacggctgt ttgccgtcac
     9121 tgaagttaaa tccgcgaccc ggcgcaatac gtgccagcat ttctgatgaa gggccatatc
     9181 ctgccgacca gaccataaac gacggtttca tttcggtcag cagttcaagg ttaggttctg
     9241 tacgcaaacc gacgtcaatc actgagtccg gcaatggtgg ttcgctgacc cacaggcgat
     9301 agttgatggt atccgccacg ccgtaaggca cgatgccgag cgccagcagt aattccaccg
     9361 gcaaccactc cagcgccaca atacgattgg ggtcaatagc cgccgcgtgg gcggtattca
     9421 tctgccataa caacggagaa agcgccatcg ccgttaacag tcgacggcgc gaaataagag
     9481 gtaagccgct catcaataaa caaaactcac aggtgcagca cccgccggat gcggcaaaat
     9541 acccatcggg atgccataaa tcatttcgag ggtttcgccg cgcataattt ccgcaggcgt
     9601 tccctgagca atcatttcac cactgcgcag ggcgaccaga taatcacagt agcgtgccgc
     9661 catattgata tcgtgcaaca cggcaatgac cgtcaggcca cgttcctgac ttaaacggtg
     9721 caccagcgcc agcacatcaa cctggtgggc gatatccagc gccgaagtcg gttcgtcgag
     9781 caacagacaa cggctatcct gcgccaccag catggcgatc cacgcccgct gacgttcgcc
     9841 gccagagaga ctatcgacca gccgatgcgc cagcggtttt aagccaacca gcgagatagc
     9901 ttcctcgacc ttttcgcgat ctgccgcacc aaagcgcccc agcgcgccat gccacgggta
     9961 acgaccaatc gccaccagtt cacgcacggt catcccttct gccggaggaa gctgctgcgg
    10021 caaataagcc actttgcggg caaacgcttt gctgctccag ctttccagcg gctgggcatc
    10081 aagaagaatc tccccttctg acggaggctg atgacgacca agcattttga gcagagtgga
    10141 tttaccagaa ccgttgtgac caatcagacc ggtcactttc ccggcaggaa aggttaacga
    10201 cagcggatgc aaaagcgtgc gcccgggcac acgaaaggag atattacgca gtgcaaaagt
    10261 ggtatcggaa tgattcgtgt attcctgcat aacagccaac ttgtgaaatg ggcacggaaa
    10321 tccgtgcccc aaaagagaaa ttagaaacgg aaggttgcgg ttgcaacgac ctgacgttct
    10381 gcgccccaga agcagccata agtctggaag caactggcaa catactcacg atcgagcaaa
    10441 ttgttgacgt tcacagcaag gctggatccc gccataccaa agcgtgccag atcatatttt
    10501 acaacagcat ccattacagc tgtgctaccc actttaaaag tgtttgccgg atcaccatag
    10561 ctggaaccaa taaaacgacc acctgttccc aatgttaaac cagaaagcgg cccttcattg
    10621 aaggtataat ccccccagag agatgccata tgctccggta cttgttctgg agttttacct
    10681 ttgagattgg tgtctttggt gtattccgca tcggtgtagg tataagaagc cgtcaagtta
    10741 atattggcat ttaacgccgc ttttgcttca agttccacac cacgagcgcg aatctcacct
    10801 gctgggactt gcgctaatgg gtttgttggg tctgctgtca ggttatttgt tttcgtcaat
    10861 tgatagactg cgcccgtaac aacgaccggc atatcattcg gaacatattt tacgccagct
    10921 tcatactgtt cacctttcga tggcttatag gaaatgcgcg ggttgctcca cagatcgaaa
    10981 gcactcggtt caaaggactg gctatagcta aagtaaggtg aaataccatt atcgaataag
    11041 taatttacac caccgcgcca ggtgaactga tgatcattgc gctcaatata accttcagtc
    11101 ggcgtataag agttttcacg aacagtggtc gcttgcttag accaatcgta gcgtcccccc
    11161 agagtgaata cccatttatt ccattccgcc tgatcctgaa cataaatacc tgtttgtttg
    11221 ctttcattca tctggtatgg ctctgcgcta ccaaatgcaa agtattcagg gtgatatttg
    11281 ttataaagat cgatggatgg tgcggatcca aagctggcat tgatatcatt acgcatccgc
    11341 atgaagtcta cccctgtcag caaagtatgc tcgacgtcac ctgtagcaaa cttactttca
    11401 agttgggtat caacgctaaa gttttgtaga cgctcattat ccaccactgt cccgcggttt
    11461 agggtatgac catcaccagc aatccctgtg ccataaacac ttttctgtga ggttttcatt
    11521 tcactgaaac gcagattctg acgcacggtg aaggtgtcat taaaaccatg ttcaaaacta
    11581 tatcccacca ttttctggtt acgggagtat gtattattcg atgcaccttc attgaagtca
    11641 gtcggtagac gcttaccatt aggcaatggc tcaacggtcc cctctttcgg caaccaaccg
    11701 taataacccg tttctggttc attctggaaa taggacagga aagtaaaatt ggttttgtcg
    11761 tctggacgcc atgagaatga tggagcaatg gtataacgct gagattcaga gtttttctgc
    11821 tgttcgtttg ttgagcgagc gaggcccgtc aaacggtaag agaactcacc gttatcatcc
    11881 agtgcatcac tgaaatcgaa tccggtctga aacagattat ccgtccccat tttaaactga
    11941 atttctttca ggggttcagt ggtcggacgc ttactcacca tagaaataat cccaccagga
    12001 ttactttttc catagagaac agacgtcggc ccacgcatca gttcaaccct ctcgagcatg
    12061 taaggatcaa tcacagcatc gttataaaag ttgccctgaa gttttagtcc atcaaggtaa
    12121 ttattttgat tcagaccaac agatgagaaa ccacgaatga taacaaaatc atatgtatta
    12181 gaagcaccac gactgctaac cgtaacgcca ggtgtataac ctaatgcttc ttttacagac
    12241 tgaaattgac gcatctgcat ctcttcattt gtcacaaccg aaatagactg cggcgttttt
    12301 tcaattggtg tatccgtttt agtcgcagta gcagagtgct tcgccgcaat agttgcagca
    12361 ggcccccatg cgctttcttg cggcgcaggt gcagcggtaa cggtgatagt gtcttctttc
    12421 ggttcaaccg ctgcctgtgc ataaacagac atgccgctaa ccgctgtggc tactacaact
    12481 gcgattttac gcagtgagtg ttttggctga gcagttttgg aacgcgccat tggtatatct
    12541 ctgatgtaaa gtgaatgata acgtaaacga gaatgattat tataaagata agataatatt
    12601 cgaaacgctg gcgggttcgc aagcggtacg caatctgagc gttcctgaag ggttaataaa
    12661 caaccagatg aaaagaaagg gttaatactt taggtgggat gttattttac cggatggcaa
    12721 ttcgccatcc ggtatttcac gcttagatgt taattactac caaacatatc cttgatccaa
    12781 ccggctacac cgtcgctgtc tttctgctct tgctgagcag gttgctgttg cggctgttgc
    12841 tgcggctgag aagactgatc aaacggattg cctgacggct gctgctgcat ctcgctctgc
    12901 tggcacagcg attgcggatc gctggtccag accggcaaga cacgcatgcc accgctgcaa
    12961 acaaagttgc cgtcgtagtc cacgcccata tctgcaatat cttctggcgg aacaagattc
    13021 agcggcgttg gcgtctggtt agccagataa cgctgataaa tagacattgc cccgctggca
    13081 ccatacagtt tggtcggctg gttgttatca cggcctaccc aggtgatggt caccgtgctg
    13141 ccgtcaatgc ccgcaaacca ggtatctacg ttattgttgg tagtccctgt tttccctgcc
    13201 agatgcaggt tcgggtattt cgccccaagt tgacgacccg taccacgttg taccacctgc
    13261 tgcatggtcc atagtgtcag atacgccgcc tgcgccggaa cagcgcgttc tgcctgcggg
    13321 aagctctgat acagcacctt gccatcttcc gcgattaccg aacgcagtgc agaaagcggt
    13381 gcacggttac cgccgctggc gatggtctgg aatgcctgcg ccacttcgat tggcgttaag
    13441 ttcaacgccc ccagcagcat cgccggaacc ggatgcagct gatctttcgg cacgcccagt
    13501 ttgatccagg tctccgtaac tgcaggcagc cccagcgcca tccccagatt taccgtcggc
    13561 acgttcatcg aacgggtcaa cgcatccacc agcatcactc tgccgctttc gctataacga
    13621 cggtcatcgt tctgcggtga ccagacctgg ccattcggct gacgcagcgc gattggcgca
    13681 tcggcaatcc acgtgttcag gcgatagatt ttcggctggc ttaaagccgt cagataagtc
    13741 gctggttttg caagggaacc aatcgaacga cgcgcctgca tcgcacggtt gtagcccgca
    13801 aactgcggct cagaacctcc caccatcgca cgaacttcac cactaaagcg gtcgacgacc
    13861 acaattgcag tttcaagatc gctcaactta cgctgtttct tcagtgccgg aatgccttcc
    13921 acggcggctt tttctgccgc gtcctgggcc accgagtcaa aggtagtgaa gatcttcacg
    13981 ccggagagat cttttacctt atcgcctaat ttcgcctgca gctcctgacg caccagttgc
    14041 ataaaggctg gctgtggaga gattacccca ccgcgcggct gtacgcccag aggacgcgcg
    14101 ctgagcattt catacagttc accatcgata atctgctgtt gttgtagcaa acgcagcacc
    14161 agattacgtc gctccagcgc cagtttcggg ttacgccacg ggttgtagat ggacgcccct
    14221 ttcaccatac cgactaacag cgcctgctgg tcgaggctga gctcttctac cgggcgacca
    14281 aagtaataca agctcgcaag cgggaagccg cggatttcgt tgtcgccgct ctgaccgaga
    14341 tacacctcgt tcatatacag ctcaagaata cggtctttgc tgtaacgcgc gtccatgatc
    14401 agcgccatgt aagcttcgtt cgctttacgc cagtaagaac gctcgctgga gaggaacagg
    14461 tttttcacca gctgttgcgt cagcgtactc gctccctgca ccgtgcgtcc ggcggtcagg
    14521 tttgccagca ccgcacgtcc gatggagtag agactgatcc catcatgctc gtagaaatga
    14581 cggtcttctg tcgccagcaa agtatccacc agcaagtccg ggaaaccact gcgcggcaca
    14641 aacagacgct gctcaccgtt tggcgaagag atcatggtga tcaggcgcgg atcaagacgg
    14701 aagaaaccga actgacggtt gttctccata ttgacgatcg tcgccagatg atcgccatca
    14761 aaggtcagac gcgcacgcac ctgcccttct ttgctgtccg ggaaatcaaa cggacggcgg
    14821 atcatctcaa tgctgttggc ctgcacggta aattcgccag gacgggtcat tttcgacacc
    14881 tgacgatact gtgttgcctc cagcagcttc accatttcgt tcttgctgat ggtcatatct
    14941 ggttcaaggt tgaccattcg gccataaact gccgcgggca gttgccagac cttgccatca
    15001 atacggctac gaattttttg atcgagataa acgccgtaaa tggcgatcag cacggcaaaa
    15061 acgatagcca gttttaacag cagccatagc cagccgcgtt tgccacgagg cttacgccct
    15121 ttgcctttgc ccttaccttt gcgcggcatc ggttcttcat cctcatagtc atcataatcg
    15181 tcgtaatcat cgtcatcttc gtaacgacga cggcttacct tttgtttgac cggacgcgtc
    15241 ggtttccctt tgcgtccaat tggctcgcgg tcattcccgg ccatgctttt tctccgcaat
    15301 attcaggcgc aaaagcccga ttttctgttc ttctgttaca agacagaaga agatatctct
    15361 caaaatttgt gctcgatcgg tcccctctcc cttgagggag agggttaggg tgagggtgaa
    15421 cgtgcgcgct gatgccctca ccccggccct ctcccacagg gagagggaga aaaccaacct
    15481 tacgaatact ttttcgtccg tcgcgtcggt gccgtgtttg ccgggtcgtc cggccagacg
    15541 tgtttgggat aacgcccttt catctctttt tgcacctcac ggtacgctcc tttccagaag
    15601 gcgctcaaat ctcgtgtgat ttgtagtggc ctttgggcgg gtgaaagcaa ctccagcacc
    15661 agcggcacgc ccccctgggc gatagtcgga ttagtggcct caccaaacat ctcctgcatt
    15721 ctcaccgcca gtgcgggcgg gttatcttca tgataacgaa tggcgatccg gcttcccgtc
    15781 ggcacagtgt aatgcgcagg caattcacta tccagacgtt gctgcattcc ccaatcaagt
    15841 aatccgcgta gtgcctgata aatgtccagt gatttcaggc cgcgtagtga atgtacgcct
    15901 gtcatatgtg gcagcagcca cgtttccagc gctgccaata aactttcatc atcaaccgct
    15961 ggccagtcat attccggcaa ccactttgcg gcgcataaca aacgcaagcg tagctgttcc
    16021 gcttccgccg tccagttgag cacgcttaaa cctttatcac ggatgccatt aagcatcgcc
    16081 tgatgcaact cgtcttctga cggtttcgcc agcggctgca ctttcaccgt caactgaccg
    16141 atttgtagcc gacgccaggc tttcagcgta ccttgcgcgt catcccattc cacggtgtca
    16201 gattgctgca ccagctgcgg acagcgttgt actaattcat caatatcgac cggtagcgcc
    16261 agtaaaatcc gcgcatccgg cgaggcgctg ccctgcaata ataacggtgc gatcaaccat
    16321 tcgtggcggc ttagcgcgtc gtcggcatcg agcatcgctc ccatgccgtt tgccagttga
    16381 tagcgtccat cttgcccacg gcgatgagca atgcgatcgg caaacgcccc ggcaagtagc
    16441 ggcgcgataa gcgaactgtc tgcctcaccg ccgcgtacgt ttaagcgttt taacagttgc
    16501 tgactacgtt gctgccaggc tggttgattg cgcgaaaacg ccacgcccag gtcactattg
    16561 cccatcagtg gcggctcttc gagaatggcg gcaatttttg ccgcggtagc agcttcgtcg
    16621 tcgttcttcg cgctaaccag cattgccgct aaacgcggat cgttacccag cgctgccatt
    16681 ttttgccctt gcgcactaag ccgttcaccg tccagcgccc ccagcatttg taacagacgt
    16741 ttcgcggcca ttagattcac tactggcggt tgatccagcc agctcatctg cgccggatcg
    16801 ctgcatcccc attgcagtaa ttccatcagc aaaccggaaa gatcgctttg taagatctcc
    16861 ggttcacttt gcgcggcagc gcgttctgct tgttctttgg cgattaaatg caggcagata
    16921 cccggctcca gacgcccggc gcgcccggca cgctgcgtca tggatgcctg gctaacgcgt
    16981 tgagtaatca gtcgcgtaag ccccgtgcgc ggctcaaaac gcgccacacg ctcctgggca
    17041 caatccacca ccagacgaat accttcaatg gttaaactgg tttcagcaat attggtcgcc
    17101 agcaccactt tgcgcatccc ttgcggtgcc gggaggatcg cttttcgctg atcgttcagc
    17161 gacaacgcgc catacagcgg gcagagcaac acatcactgc cgatgcgcga agccagttgt
    17221 tcctgcacac gctgaatttc tccgacgcca ggtaaaaata acagtaatga tccgctttcc
    17281 tgacgcagca tttcggcggt ggctaccgca acggcttcgt caaaacgctg atgcgcgggc
    17341 agcggtaaat aatggcgttc aaccggaaac gagcgccctt ctgagatgac gacgggcgct
    17401 tctggcagca tttgctgcaa gcggtcgtta tccagcgtag ccgacataat cagcagttta
    17461 aggtcatcac gcagaccttg ttgcacatcg agtaacagcg ccaacgccaa atccgcctgc
    17521 aagctgcgct catgaaattc atcaaggatc accaatccaa caccgctcaa ctccgggtca
    17581 cgctggatca tccgcgtcag cacgccttcg gtaactactt ccagacgggt atttggcccg
    17641 acgcagtttt gcgcacgcat ccggtagcca acggtatcgc ctggcttttc gttaagcagc
    17701 tccgccagcc gttgcgcgac gttacgtgcc gccagacgac gcggctccag caggataatt
    17761 ttcccgttaa tgccgggatg cgccagcagt tgcagcggca gccaggttga tttcccggcc
    17821 ccggtcggcg cacttaataa tacctgcggc gcacaatcga gagcggtaag taattcaggt
    17881 aagacggcag caacgggcaa cgacgacaca aaacgctcca gagggttaac attcttcgcg
    17941 ccacattgta gcatcgcggt aattcataac cgagtgcctc acatgtctga accgcaacgt
    18001 ctgttctttg ctatcgactt acctgcagaa atccgcgaac agattatcca ctggcgcgcc
    18061 acacacttcc cacctgaggc gggacgtccg gtcgccgccg ataatttgca tctgactctg
    18121 gcatttttag gcgaagtgag cgccgagaaa gagaaggcgc tttctctttt agccggacgg
    18181 attcgtcaac ctggtttcac actcacgctt gatgacgccg gacaatggct gcgttcgcgt
    18241 gtggtgtggt tagggatgcg tcagccgcca cgcggcttaa tccagctggc gaatatgctc
    18301 cgttcacagg ctgcccgcag cggttgtttt caaagcaatc gtccgtttca tccacatatt
    18361 accttattgc gcgacgccag cgaggcggtg acaatcccgc cgccaggttt taactggtct
    18421 tatgcggtga cggagttcac cctttacgcc tcctcgtttg cccgtggacg cacacgctac
    18481 acgccgctaa aacgctgggc gctaacgcaa taacaaggat tgtcgcaatg gaattctctc
    18541 cccctctaca gcgcgcgacg ctaattcagc gttacaaacg ttttttagcc gatgtgatca
    18601 cacccgatgg tcgcgaatta acgctacact gcccgaatac gggtgcgatg accggttgtg
    18661 caacgcgtgg cgataccgtc tggtattcga cttcagacaa caccaaacgg aaatacccac
    18721 acacctggga actaactcaa agccagagcg gcgcatttat ttgcgtcaat acgctttggg
    18781 ctaacaggtt gacgaaagag gctatcctta atgaatcaat ttcagaactg tcaggctata
    18841 gctcgctgaa aagcgaagta aaatacggcg cagaacgcag ccgtattgac tttatgttgc
    18901 aggcggattc gcgtccagac tgctatattg aagtgaaatc ggttacgtta gcggagaacg
    18961 aacagggata ttttcccgat gcggtcactg aacgaggtca gaaacacctt cgggagttga
    19021 tgaacgtagc ggctgaaggc cagcgtgcgg ttatcttttt cgccgtgctg cattcagcca
    19081 ttacacggtt ttcacccgcg cgccacatcg atgagaaata cgcgcaacta ttgtcagaag
    19141 ctcaacagag gggggtagaa attctggctt acaaagcgga aatttctgct gaaggcatgg
    19201 ctcttaaaaa atcactgtcg gttacattgt agtaaagtaa gtaactggtt aatttacatt
    19261 ctggtcgcgt gcgcaaatac gcttttcctc acacagttgt caagtgttac gtttagataa
    19321 ttgctatccg gaaaagcatc tgctatttat agcggcctca tttttccccc gaacatgggg
    19381 atcgatagtg cgtgttaagg agaagcaaca tgcaagaagg gcaaaaccgt aaaacatcgt
    19441 ccctgagtat tctcgccatc gctggggtgg aaccatatca ggagaagccg ggcgaagagt
    19501 atatgaatga agcccagctg gcgcacttcc gtcgtattct ggaagcatgg cgtaatcaac
    19561 tcagggatga agtcgatcgc accgttacac atatgcagga tgaagcagcc aacttcccgg
    19621 acccggtaga ccgtgcagcc caggaagaag agttcagcct cgaactgcgt aaccgcgatc
    19681 gcgagcgtaa gctgatcaaa aagatcgaga agacgctgaa aaaagtggaa gacgaagatt
    19741 tcggctactg cgaatcctgc ggtgttgaaa ttggtattcg ccgtctggaa gcgcgcccga
    19801 cagccgatct gtgcatcgac tgcaaaacgc tggctgaaat tcgcgaaaaa cagatggctg
    19861 gctaattaca gccgttccat cacgtttacc acaggcggga aatgctcccg ccttattttt
    19921 tgttcaaaga gatgacagac acacagtata ttggccgctt cgccccctct ccttccggcg
    19981 agcttcattt tggctctctg atcgccgcgc tcggcagcta tttgcaggct cgcgcccggc
    20041 aaggtcgctg gctggtacgc atagaagata tcgacccgcc tcgtgaagtt cccggtgccg
    20101 cagaaactat cctgcgccag ctggaacatt acggtctgca ctgggacggc gatgttctct
    20161 ggcaatcgca acgtcacgac gcctatcgtg aagcactcgc ctggttacat gaacaaggac
    20221 taagttatta ctgcacctgt acgcgtgcgc gtattcaaag cattggcggt atttacgacg
    20281 gtcattgccg ggtgttgcat catggaccag acaatgccgc agtgcgtatc cgccagcagc
    20341 atccggtcac gcaatttact gaccagctgc gcggcattat tcacgccgac gaaaaactgg
    20401 cacgggaaga ttttatcatt catcgccgtg atgggttgtt cgcctacaac ctggctgttg
    20461 tggttgatga tcatttccag ggcgttacag aaatagtgcg tggggctgat ctgattgaac
    20521 caacagtaag gcaaatctcg ctgtaccagc tttttggctg gaaagtgcca gattacattc
    20581 atctgccgct ggcgcttaat ccacaaggcg ctaaactttc caagcagaat catgcgcctg
    20641 cgttgccgaa aggcgatcca cgtccggtac taatcgcggc acttcaattt ctgggtcagc
    20701 aggcagaagc acactggcag gatttcagcg tcgaacaaat ccttcagtca gcagtcaaaa
    20761 actggacgct gaccgccgtg cccgagtcgg caattgtaaa ttcaacattc tcaaatgcgt
    20821 catgctgagc tatgattagc cgctattttt ttgtcctgaa tgatgtttga cactaccgag
    20881 gtgtactatt tttacccgag tcgctaattt ttgccgcaag gtgctaagcc gcgaggaaag
    20941 cgaggctgaa caggcagtcg cccgtccaca ggtgacggtg atcccgcgtg agcagcatgc
    21001 tatttcccgc aaagatatca gtgaaaatgc cctgaaggta atgtacaggc tcaacaaagc
    21061 gggatacgaa gcctggctgg ttggcggcgg cgtgcgcgac ctgttacttg gcaaaaagcc
    21121 gaaagatttt gacgtaacca ctaacgccac gcctgagcag gtgcgcaaac tgttccgtaa
    21181 ctgccgcctg gtgggtcgcc gtttccgtct ggctcatgta atgtttggcc cggagattat
    21241 cgaagttgcg accttccgtg gacaccacga aggtaacgtc agcgaccgca cgacctccca
    21301 acgcgggcaa aacggcatgt tgctgcgcga caacattttc ggctccatcg aagaagacgc
    21361 ccagcgccgc gatttcacta tcaacagcct ctattacagc gtagcggatt ttaccgtccg
    21421 tgattacgtt ggcggtatga aggatctgaa ggacggcgtt atccgtctga ttggtaaccc
    21481 ggaaacgcgc taccgtgaag atccggtacg gatgctgcgc gcggtacgtt ttgccgccaa
    21541 attgggtatg cgcatcagcc cggaaaccgc agaaccgatc cctcgcctcg ctaccctgct
    21601 gaacgatatc ccaccggcac gcctgtttga agaatcgctt aaactgctac aagcgggcta
    21661 cggttacgaa acctataagc tgttgtgtga atatcatctg ttccagccgc tgttcccgac
    21721 cattacccgc tacttcacgg aaaatggcga cagcccgatg gagcggatca ttgaacaggt
    21781 gctgaagaat accgatacgc gtatccataa cgatatgcgc gtgaacccgg cgttcttgtt
    21841 tgccgccatg ttctggtacc cactgctgga gacggcacag aagatcgccc aggaaagcgg
    21901 cctgacctat cacgacgctt tcgcgctggc gatgaacgac gtgctggacg aagcctgccg
    21961 ttcactggca atcccgaaac gcctgacgac gttaacccgc gatatctggc agttgcagtt
    22021 gcgtatgtcc cgacgtcagg gtaaacgcgc atggaaactg ctggagcatc ctaagttccg
    22081 tgcggcttat gacctgttgg ccttgcgagc tgaagttgaa cgtaacgctg aactgcagcg
    22141 tctggtgaaa tggtggggtg agttccaggt ttccgcgcca ccagatcaaa aagggatgct
    22201 taacgagttg gatgaagagc cgtcaccgcg tcgccgtact cgtcgtccac gcaaacgcgc
    22261 accgcgtcgt gagggtaccg catgacagtg gcgtatattg ccataggcag caatctggcc
    22321 tctccgctgg agcaggtcaa tgctgccctg aaagcattag gcgatatccc tgaaagccac
    22381 attcttgccg tttcttcgtt ttaccgcact ccaccgctgg ggccgcaaga tcaacccgat
    22441 tacctcaacg ccgccgtggc gcttgaaact tcgctcgccc cggaagagtt actcaaccat
    22501 acacagcgca tagaactgca acagggtcgt gttcgtaaag ctgaacgctg gggaccacgc
    22561 acgctggatc tcgatatcat gctgtttggt aatgaagtga taaataccga acgcctgacc
    22621 gttccgcact acgatatgaa gaatcgtgga tttatgctgt ggccgctgtt tgagattgca
    22681 ccggagctgg cgtttcctga tggggagacg ttgcgtgaag tcttacatac aagagcattt
    22741 gacgaattaa gcaaatggta atttatttat attacgaata taaatatttt ctgaaaatca
    22801 tacatcctct ctttccttaa taaaaatttt agtttaaaca tttttgcaga aacataatat
    22861 aaattcccgc agataaatat caatcccatc gccataccct aaccaattgc acttgtgaat
    22921 ttatttttgc ttaattttga attaagaaaa tttaatttgt tcaaaagccc ttaaagtgca
    22981 caataggcgt cctaatgaac aatggttgta aattttggta acaaatatca ttgctcatta
    23041 atatctagca tctgcttata acatttgtgt catgaatagt cattcttcaa accagtagtc
    23101 tgactaatgg aattttattc aaggattttt atgtctaaaa aattaggttt tgccctgtct
    23161 ggtcttatgc tggcaatggt tgcaggtact gcttctgctg atatggatgg tggtcagtta
    23221 aatatcagtg gtctggttgt tgataacacc tgtgaaaccc gcgttgacgg cggtaataaa
    23281 gatggtctga tcctgctgca aactgcaact gttggtgaaa tcgatgctgg cgtgctgaat
    23341 gacaccgttg gcgctaaagc caaacctttc agcatcaccg ttgactgcag caaagcaaat
    23401 ccgaatccag gtagtacagc taaaatgacc ttcggttctg ttttctttgg taatagcaaa
    23461 ggaacgctga acaacgacat gtctattaac aacccgtctg acggcgtgaa tatcgctctg
    23521 cacaatattg atggttccac tatcaaacag gttcaaatta acaaccctgg cgatgtgtat
    23581 actaaagccc tggatgcaac gacaaaatct gctgtttatg attttaaagc gtcttacgtt
    23641 cgtgccgtcg cagaccaaac agcaactgct ggttatgtaa aaactaacac tgcatacacc
    23701 attacttatc agtaagtatt caccaattaa attgattgaa tgaatataca gggaatagtg
    23761 ttaataattt ctatttttat attattccct gttttaatta actctgtcag ggatggttta
    23821 tgttttttaa caccaaacat acaacagctt tatgcttcgt aacctgtatg gcttttagtt
    23881 catcgtctat tgcggacatt gtcatttcgg gtactcgcgt aatatataaa agcgatcaaa
    23941 aaagtgtcaa cgtacgtctg gaaaataaag ggaataaccc gttgcttgtc cagagttggt
    24001 tagatactgg cgataacaac gctgagcctg gcagtattac agtccctttt actgccacgc
    24061 cgccagtatc gcgtattgat gccaaacgtg ggcaaacaat caaattaatg tacacagcca
    24121 gcacctcact gcctaaagac agagagagcg tgttctggtt taacgtactg gaagttccac
    24181 caaaaccaga tgcagaaaag gtcgcgaatc aaagtctgct gcaactggca tttcgcacac
    24241 gtataaaact tttctatcgc ccggatggat tgaagggaaa tccctctgaa gccccgttag
    24301 ccctgaagtg gttctggtca ggttcagaag gtaaggcgtc attgcgagtg accaatccaa
    24361 ccccttacta cgtctctttt agcagtggtg atttagaagc tagcggtaaa cgctatccga
    24421 ttgatgtgaa aatgattgca ccatttagtg atgaggtcat gaaagtcact ggccttaatg
    24481 gcaaagcaag ttctgcaaaa gtacattttt acgccattaa tgattttggt ggcgccattg
    24541 aaggtaatgc cagtctgtaa tcaggcaagg atataattcc gcaggaagca taacgtgact
    24601 ataaaaccaa ctaaaaatta tcataatcat ctgacccgta tcgctacgtt ttgcgcgctg
    24661 ctgtattgca atagtgcttt ctgtgctgaa cttgttgaat atgaccatac cttcctgatg
    24721 gggcagaatg cctcaaatat tgatctcagc cggtatagtg aaggtaaccc tgctataccg
    24781 ggtatgtacg acgtcagtgt ttatgtcaac gatcaaccta ttattaacca aagcattaca
    24841 tttattgaaa ttgaaggaaa aaaaacgcac aagcctgtat cacacttaag aatttattgc
    24901 agtttcatat aaattctccc gatataaata acgaaaaagc cgttctgctt gccagggatg
    24961 aaacactcgg caattgcctt aatttgacgg aaattatccc tcaggcttct gttcgttatg
    25021 acgttaacga gcaacgtctg gatatagacg ttcctcaggc atgggtaatg aaaaattacc
    25081 aaaactatgt tgatccatcg ttatgggaaa atggcattaa tgcggccatg ttgtcataca
    25141 acctcaacgg atatcatagt gaaactcctg gtcgaagaaa tgacagcatt tatgctgctt
    25201 ttaacggtgg gatgaattta ggtgcatggc gactgcgtgc ctcaggcaac tacaactgga
    25261 tgaccgattc tggcagtaat tatgatttta agaatcggta tatacagcgt gatatcgcct
    25321 cgctgcgttc tcaactcatt cttggtgagt cttatacaac gggcgaaacc tttgattctg
    25381 tcagtatccg cggcattcgt ttatacagcg acagccgcat gttgcctccg acattagcga
    25441 gttttgcgcc tatcattcat ggcgttgcta ataccaacgc caaagtcact attacgcaag
    25501 gtggctataa gatttatgaa acgactgtgc cgccaggcgc tttcgtcatt gatgatctga
    25561 gtccgtcagg ttacggcagc gatcttattg ttactgtcga agaatccgat ggctcaaagc
    25621 ggacattctc gcaacctttt tcatcggtcg ttcaaatgtt acgccctggc gttggacgtt
    25681 gggatattag cggcggccag gtcttaaaag atgatattca ggatgagcct aatttatttc
    25741 aggccagcta ctactacggt ctgaataact atctaacg
//
LOCUS       DAJTXQ010000050        25496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000050 DAJTXQ010000000
VERSION     DAJTXQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.050"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            17..487
                     /locus_tag="OO515_001990"
     CDS             17..487
                     /locus_tag="OO515_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6575930.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            489..908
                     /gene="gspB"
                     /locus_tag="OO515_001991"
     CDS             489..908
                     /gene="gspB"
                     /locus_tag="OO515_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCR6575931.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1146..1457
                     /gene="rpsJ"
                     /locus_tag="OO515_001992"
     CDS             1146..1457
                     /gene="rpsJ"
                     /locus_tag="OO515_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6575932.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1490..2119
                     /gene="rplC"
                     /locus_tag="OO515_001993"
     CDS             1490..2119
                     /gene="rplC"
                     /locus_tag="OO515_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6575933.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2130..2735
                     /gene="rplD"
                     /locus_tag="OO515_001994"
     CDS             2130..2735
                     /gene="rplD"
                     /locus_tag="OO515_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6575934.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2732..3034
                     /gene="rplW"
                     /locus_tag="OO515_001995"
     CDS             2732..3034
                     /gene="rplW"
                     /locus_tag="OO515_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6575935.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3052..3873
                     /gene="rplB"
                     /locus_tag="OO515_001996"
     CDS             3052..3873
                     /gene="rplB"
                     /locus_tag="OO515_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6575936.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3890..4168
                     /gene="rpsS"
                     /locus_tag="OO515_001997"
     CDS             3890..4168
                     /gene="rpsS"
                     /locus_tag="OO515_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6575937.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4183..4515
                     /gene="rplV"
                     /locus_tag="OO515_001998"
     CDS             4183..4515
                     /gene="rplV"
                     /locus_tag="OO515_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6575938.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4533..5234
                     /gene="rpsC"
                     /locus_tag="OO515_001999"
     CDS             4533..5234
                     /gene="rpsC"
                     /locus_tag="OO515_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6575939.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5247..5657
                     /gene="rplP"
                     /locus_tag="OO515_002000"
     CDS             5247..5657
                     /gene="rplP"
                     /locus_tag="OO515_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6575940.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5657..5848
                     /gene="rpmC"
                     /locus_tag="OO515_002001"
     CDS             5657..5848
                     /gene="rpmC"
                     /locus_tag="OO515_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6575941.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5848..6102
                     /gene="rpsQ"
                     /locus_tag="OO515_002002"
     CDS             5848..6102
                     /gene="rpsQ"
                     /locus_tag="OO515_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6575942.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6267..6638
                     /gene="rplN"
                     /locus_tag="OO515_002003"
     CDS             6267..6638
                     /gene="rplN"
                     /locus_tag="OO515_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6575943.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6649..6963
                     /gene="rplX"
                     /locus_tag="OO515_002004"
     CDS             6649..6963
                     /gene="rplX"
                     /locus_tag="OO515_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6575944.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6978..7517
                     /gene="rplE"
                     /locus_tag="OO515_002005"
     CDS             6978..7517
                     /gene="rplE"
                     /locus_tag="OO515_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6575945.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7532..7837
                     /gene="rpsN"
                     /locus_tag="OO515_002006"
     CDS             7532..7837
                     /gene="rpsN"
                     /locus_tag="OO515_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6575946.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7871..8263
                     /gene="rpsH"
                     /locus_tag="OO515_002007"
     CDS             7871..8263
                     /gene="rpsH"
                     /locus_tag="OO515_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6575947.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8276..8809
                     /gene="rplF"
                     /locus_tag="OO515_002008"
     CDS             8276..8809
                     /gene="rplF"
                     /locus_tag="OO515_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6575948.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8819..9172
                     /gene="rplR"
                     /locus_tag="OO515_002009"
     CDS             8819..9172
                     /gene="rplR"
                     /locus_tag="OO515_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6575949.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9187..9690
                     /gene="rpsE"
                     /locus_tag="OO515_002010"
     CDS             9187..9690
                     /gene="rpsE"
                     /locus_tag="OO515_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6575950.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9694..9873
                     /gene="rpmD"
                     /locus_tag="OO515_002011"
     CDS             9694..9873
                     /gene="rpmD"
                     /locus_tag="OO515_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6575951.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9877..10311
                     /gene="rplO"
                     /locus_tag="OO515_002012"
     CDS             9877..10311
                     /gene="rplO"
                     /locus_tag="OO515_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6575952.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10319..11650
                     /gene="secY"
                     /locus_tag="OO515_002013"
     CDS             10319..11650
                     /gene="secY"
                     /locus_tag="OO515_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6575953.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11682..11798
                     /gene="rpmJ"
                     /locus_tag="OO515_002014"
     CDS             11682..11798
                     /gene="rpmJ"
                     /locus_tag="OO515_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6575954.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11945..12301
                     /gene="rpsM"
                     /locus_tag="OO515_002015"
     CDS             11945..12301
                     /gene="rpsM"
                     /locus_tag="OO515_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6575955.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12318..12707
                     /gene="rpsK"
                     /locus_tag="OO515_002016"
     CDS             12318..12707
                     /gene="rpsK"
                     /locus_tag="OO515_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6575956.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12741..13361
                     /gene="rpsD"
                     /locus_tag="OO515_002017"
     CDS             12741..13361
                     /gene="rpsD"
                     /locus_tag="OO515_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6575957.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13387..14376
                     /gene="rpoA"
                     /locus_tag="OO515_002018"
     CDS             13387..14376
                     /gene="rpoA"
                     /locus_tag="OO515_002018"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6575958.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14417..14800
                     /gene="rplQ"
                     /locus_tag="OO515_002019"
     CDS             14417..14800
                     /gene="rplQ"
                     /locus_tag="OO515_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6575959.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14907..15275
                     /locus_tag="OO515_002020"
     CDS             14907..15275
                     /locus_tag="OO515_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6575960.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15286..15711
                     /gene="zntR"
                     /locus_tag="OO515_002021"
     CDS             15286..15711
                     /gene="zntR"
                     /locus_tag="OO515_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6575961.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15767..15985
                     /gene="arfA"
                     /locus_tag="OO515_002022"
     CDS             15767..15985
                     /gene="arfA"
                     /locus_tag="OO515_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6575962.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15982..16392)
                     /gene="mscL"
                     /locus_tag="OO515_002023"
     CDS             complement(15982..16392)
                     /gene="mscL"
                     /locus_tag="OO515_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6575963.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16522..17898)
                     /gene="trkA"
                     /locus_tag="OO515_002024"
     CDS             complement(16522..17898)
                     /gene="trkA"
                     /locus_tag="OO515_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6575964.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17920..19209)
                     /gene="rsmB"
                     /locus_tag="OO515_002025"
     CDS             complement(17920..19209)
                     /gene="rsmB"
                     /locus_tag="OO515_002025"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6575965.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19255..20202)
                     /gene="fmt"
                     /locus_tag="OO515_002026"
     CDS             complement(19255..20202)
                     /gene="fmt"
                     /locus_tag="OO515_002026"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6575966.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20217..20726)
                     /gene="def"
                     /locus_tag="OO515_002027"
     CDS             complement(20217..20726)
                     /gene="def"
                     /locus_tag="OO515_002027"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6575967.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20856..21980
                     /gene="dprA"
                     /locus_tag="OO515_002028"
     CDS             20856..21980
                     /gene="dprA"
                     /locus_tag="OO515_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6575968.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21952..22425
                     /locus_tag="OO515_002029"
     CDS             21952..22425
                     /locus_tag="OO515_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6575969.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22454..22996
                     /locus_tag="OO515_002030"
     CDS             22454..22996
                     /locus_tag="OO515_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6575970.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23001..23573
                     /gene="tsaC"
                     /locus_tag="OO515_002031"
     CDS             23001..23573
                     /gene="tsaC"
                     /locus_tag="OO515_002031"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6575971.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23578..24396
                     /gene="aroE"
                     /locus_tag="OO515_002032"
     CDS             23578..24396
                     /gene="aroE"
                     /locus_tag="OO515_002032"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6575972.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24393..24650
                     /locus_tag="OO515_002033"
     CDS             24393..24650
                     /locus_tag="OO515_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6575973.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24626..25180)
                     /locus_tag="OO515_002034"
     CDS             complement(24626..25180)
                     /locus_tag="OO515_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6575974.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6022 c         6680 g         6302 t
ORIGIN      
        1 tgcttcttta caaacattgg tagatcagaa tttgccctgg agcgcatcgc ttaaagtcgg
       61 ggacaaaaaa ttgcctgttg tggtcgttcg cgtcggtgag gctagcgttg atgtcctcgt
      121 aggtcagcaa acgtggacgc tgacacataa atggtttgag tcggtatgga cgggtgatta
      181 tcttctgttg tggaaaatgt cgccggaagg ggagagtacc attacgcgtg atagcagcga
      241 ggaagagatt ctctggctgg aaacgatgtt aaaccgtgca ttgcatattt cgacagaacc
      301 ttcggcggag tggcgtccct tgttggtaga aaaaataaag caatttcaaa aaagccacca
      361 cttgaaaact gacggtgttg ttggcttttc aacgttagta catctctggc aggtggcagg
      421 ggagagtgcc tacttatatc gggatgaagc aaatatttcc ccggaaacaa cagtgaaggg
      481 gaaatgacat gtttgaattc tatatcgccg cgcgtgagca gaaagaaaca ggtcaccctg
      541 gtatcttctc ccgacaaaaa cacagcacca taatatatgt catttgttta ttgctgattt
      601 gcctgtggtt tgcaggaatg gtgttggttg gtgggtatgc caggcagcta tgggcgcttt
      661 ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc cgcttttaaa caatcaacac
      721 aacactattt cttcaaaaaa cagccactcc ccgtcgtcga atccgtcgag gaggaagatg
      781 acccagacgt cgcggttgag aatgctccct cttcttctga agacgaagaa aatactgtaa
      841 aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa aaatgcgctg aacgaacttg
      901 agagataacc cgaaggctgt ttactttact agcaatacgc ttgcgttcgg tggttaagta
      961 tgtataatgc gcgggcttgt cgtagttgac agcaggttca atctgaacct cagttagccg
     1021 aatttggcta cctaacaatg ctcccaatcg gggagctacg taagaacggt tacactctcc
     1081 catcaatcgt aatgggtctg aggagtaatc attttcgttt ataaaataat tggagctctg
     1141 gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg
     1201 atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac tggtgcgcag gtccgtggtc
     1261 cgatcccgct gccgacacgc aaagagcgct tcactgttct gatctccccg cacgtcaaca
     1321 aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg tctggttgac atcgttgagc
     1381 caaccgagaa aaccgttgat gctctgatgc gtctggatct ggctgccggt gtagacgtgc
     1441 agatcagcct gggttaatca ggtcattgag cgattgagag gttgaaacaa tgattggttt
     1501 agtcggtaaa aaagtgggta tgacccgtat cttcacagaa gacggcgttt ctatcccagt
     1561 aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg
     1621 ctaccgtgct attcaggtga ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga
     1681 agctggccac ttcgctaaag ctggcgtaga agctggccgt ggtctgtggg aattccgcct
     1741 ggctgaaggc gaagagttca ctgtaggtca gagcattagc gttgaactgt ttgctgaggt
     1801 taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg
     1861 ctggaacttc cgtacccagg acgctactca cggtaactcc ttgtctcacc gcgttccggg
     1921 ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca
     1981 gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta gtacgcgttg acgctgagcg
     2041 caacctgctg ctggttaaag gtgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa
     2101 accagctgtg aaggcgtaag gagatagcaa tggaattagt attgaaagac gcgcagagcg
     2161 cgctgactgt ttccgaaact accttcggtc gtgatttcaa cgaagcgctg gttcaccagg
     2221 ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg tgctcagaag actcgtgctg
     2281 aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg caccggccgt gcgcgttctg
     2341 gttctatcaa gagcccgatc tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg
     2401 accacagtca aaaagttaac aagaagatgt accgcggcgc gctgaaaagc atcctgtccg
     2461 aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa
     2521 ctaagctgct ggcacagaaa ctgaaagaca tggctctgga agatgtgctg atcatcaccg
     2581 gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct gcacaaggtt gacgtacgcg
     2641 atgcaactgg tatcgacccg gttagcctga tcgccttcga caaagtcgta atgactgctg
     2701 atgctgttaa gcaagttgag gagatgctgg catgattcgt gaagaacgtc tgctgaaggt
     2761 gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg atggaaaaat ccaacaccat
     2821 cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc aaagctgctg tgcagaaact
     2881 gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt aaagggaaag ttaaacgtca
     2941 cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg
     3001 ccagaatctg gacttcgttg gcggcgctga gtaagtcgga ggagtaatac aatggcagtt
     3061 gttaaatgta aaccgacatc tccgggtcgt cgccacgtag ttaaagtggt taaccctgag
     3121 ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt
     3181 aacaacaatg gccgtatcac cactcgtcat atcggtggtg gccacaagca ggcttaccgt
     3241 attgttgact tcaaacgcaa caaagacggt atcccggcag ttgttgaacg tcttgagtac
     3301 gatccgaacc gttccgcgaa catcgcgctg gttctgtaca aagacggtga acgccgttac
     3361 atcctggccc ctaaaggcct gaaagctggc gaccagattc agtctggcgt tgatgctgca
     3421 atcaaaccag gtaacaccct gccgatgcgc aacatcccgg ttggttctac tgttcataac
     3481 gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt ccgctggtac ttacgttcag
     3541 atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa
     3601 gtagaagcag actgccgtgc aactctgggc gaagttggca atgctgagca tatgctgcgc
     3661 gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc
     3721 gcgatgaacc cggtagacca cccacatggt ggtggtgaag gtcgtaactt tggtaagcac
     3781 ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact
     3841 gataaattca tcgtacgtcg ccgtagcaaa taattttaga ggataagcca tgccacgttc
     3901 tctcaagaaa ggtcctttta ttgacctgca cttgctgaag aaggtagaga aagcggtgga
     3961 aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat
     4021 gatcggtttg accatcgctg tccataatgg tcgtcagcac gttccggtat ttgtgaccga
     4081 cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact cgtacttatc gcggccacgc
     4141 tgctgataaa aaagcgaaga agaaataagg taggaggaag agatggaaac tatcgctaaa
     4201 catcgccatg ctcgttcttc tgctcagaag gttcgccttg ttgctgacct gattcgcggt
     4261 aagaaagtgt cgcaggctct ggatattttg acctacacca acaagaaagc ggctgtactg
     4321 gtcaagaaag ttctggaatc tgccattgct aacgctgaac acaacgatgg cgctgacatt
     4381 gacgatctga aagttacgaa aattttcgta gacgaaggcc cgagcatgaa gcgcattatg
     4441 ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca ccagccacat cactgtggtt
     4501 gtgtccgatc gctgagactc tggagactag caatgggtca gaaagtacat cctaatggta
     4561 ttcgcttggg tattgtaaaa ccatggaact ctacctggtt tgcgaacacc aaagaattcg
     4621 ctgacaacct ggacagcgat tttaaagtac gtcagtacct gactaaggaa ctggctaaag
     4681 cgtctgtatc tcgtatcgtt atcgagcgtc cggctaagag catccgtgta accattcaca
     4741 ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg
     4801 tcgtagcgga catcgctggc gttcctgcac agatcaacat cgccgaagtt cgtaagcctg
     4861 aactggacgc aaaactggtt gctgacagca tcacttctca gctggaacgt cgcgttatgt
     4921 tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg tctgggcgct aaaggtatta
     4981 aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc acgtaccgaa tggtaccgcg
     5041 aaggtcgcgt accgctgcac actctgcgtg ctgacatcga ctacaacacc tctgaagcgc
     5101 acaccactta cggtgtaatc ggcgttaaag tgtggatctt caaaggcgag atcctgggtg
     5161 gtatggctgc tgttgaacaa ccggaaaaac cggctgctca gcctaaaaag cagcagcgta
     5221 aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa
     5281 tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga tgttagcttc ggcagcttcg
     5341 gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca gatcgaagca gcacgtcgtg
     5401 ctatgacccg tgcagttaag cgtcaaggta agatctggat ccgtgtgttc ccggacaaac
     5461 cgatcactga aaagccgctg gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt
     5521 gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc
     5581 tggcccgtga agcattcaag ctggcagcag cgaaactgcc gattaaaacc acctttgtaa
     5641 ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag aagagcgttg aagagctgaa
     5701 caccgagctg ctgaacctgc tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg
     5761 ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac
     5821 tttactgaac gagaaggcgg gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc
     5881 gttgttagcg acaaaatgga gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac
     5941 ccgatctacg gtaaattcat caagcgtacg accaaactgc acgtacatga cgagaacaac
     6001 gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa
     6061 tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt aatacagtac actctctcga
     6121 tacgaataaa cggctcagaa atgagccgtt tattttttct acccatatcc ttgaagcggt
     6181 gttataatgc cgcgccctcg atatggggat ttttaacgac ctgattttcg ggtctcagta
     6241 gtagttgaca ttagcggagc actaaaatga tccaagaaca gactatgctg aacgtcgccg
     6301 acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct gggtggctcg caccgtcgct
     6361 acgcaggcgt aggcgacatc atcaagatca ccatcaaaga agcaattccg cgtggtaagg
     6421 tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc
     6481 cggacggttc tgtcattcgc ttcgatggta atgcttgtgt tcttctgaac aacaacagcg
     6541 agcagcctat cggtacgcgt atttttgggc cggtaactcg tgagcttcgt agtgagaagt
     6601 tcatgaaaat tatctctctg gcaccagaag tactctaagg agcgaatcat ggcagcgaaa
     6661 atccgtcgtg atgacgaagt tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa
     6721 gttaagaatg tcctgtcttc cggcaaggtc attgttgaag gtatcaacct ggttaagaaa
     6781 catcagaagc cggttccggc cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct
     6841 attcaggttt ccaacgtagc aatcttcaat gcggcaaccg gcaaggctga ccgtgtaggc
     6901 tttagattcg aagacggcaa aaaagtccgt ttcttcaagt ctaacagcga aactatcaag
     6961 taatttggag tagtacgatg gcgaaactgc atgattacta caaagacgaa gtagttaaaa
     7021 aactcatgac tgagtttaac tacaattctg tcatgcaagt ccctcgggtc gagaagatca
     7081 ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa actgctggat aacgcagcag
     7141 cagacctggc agcaatctcc ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg
     7201 caggcttcaa aatccgtcag ggctatccga tcggctgtaa agtaactctg cgtggcgaac
     7261 gcatgtggga gttctttgag cgcctgatca ctattgctgt acctcgtatt cgtgacttcc
     7321 gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc
     7381 agatcatctt cccagaaatc gactacgata aagtcgaccg cgttcgtggt ttggatatta
     7441 ccattaccac tactgcgaaa tctgacgaag aaggccgcgc tctgctggct gcctttgact
     7501 tcccgttccg caagtaaggt agggttacta aatggctaag caatcaatga aagcacgcga
     7561 agtaaaacgc gtagctttag ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat
     7621 catctctgat gtgaacgctt ccgacgaaga tcgttggaac gctgttctca agctgcagac
     7681 tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgccc
     7741 gcatggtttc ctgcggaagt tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg
     7801 cggtgaaatc ccgggtctga aaaaggctag ctggtaattg tcaccaattg aatcacggga
     7861 gtaaagacag atgagcatgc aagatccgat cgcggatatg ctgacccgta tccgtaacgg
     7921 tcaggccgcg aacaaagctg cggtcaccat gccttcctcc aagctgaaag tggcaatcgc
     7981 caacgtgctg aaggaagaag gttttattga agattttaaa gttgaaggcg acaccaagcc
     8041 tgaactggaa cttactctga agtatttcca gggcaaagct gttgtagaaa gcattcagcg
     8101 tgtcagccgc ccaggtctgc gcatctataa acgtaaagat gagctgccga aagttatggc
     8161 gggtctgggt atcgcagttg tttctacctc taaaggtgtt atgactgatc gtgcagcgcg
     8221 ccaggctggt cttggtggcg aaattatctg ctacgtagcc taatcggagg aaaaaatgtc
     8281 tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca
     8341 ggttattacg atcaaaggta aaaacggcga gctgactcgt actctcaacg atgctgttga
     8401 agttaaacat gcagataata ccctgacctt cggtccgcgt gatggttacg cagacggttg
     8461 ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg gttatcggtg ttaccgaagg
     8521 cttcactaag aagctgcagc tggttggtgt aggttaccgt gcagcggtta aaggcaatgt
     8581 gattaacctg tctctgggtt tctctcatcc tgttgaccat cagctgcctg cgggtatcac
     8641 tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc gttgataagc aggtgatcgg
     8701 ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag ccttataaag gcaagggtgt
     8761 tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag aagaagtaag gtaacactat
     8821 ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct
     8881 gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac atttacgcac aggtaattgc
     8941 accgaacggt tctgaagttc tggtagctgc ttctactgta gaaaaagcta tcgctgaaca
     9001 actgaagtac accggtaaca aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg
     9061 cgctctggaa aaaggcatca aagatgtatc ctttgaccgt tccgggttcc aatatcatgg
     9121 tcgtgtccag gcactggcag atgctgcccg tgaagctggc cttcagttct aaggtagagg
     9181 tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc aggaaaagct gatcgcggta
     9241 aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct ccttcacagc tctgactgta
     9301 gttggcgatg gtaacggtcg cgttggtttt ggttacggta aagcgcgtga agttccagca
     9361 gcgatccaga aagcgatgga aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac
     9421 ggcactctgc aacaccctgt taaaggtgtt cacacgggtt ctcgcgtatt catgcagccg
     9481 gcttccgaag gtaccggtat catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct
     9541 ggggttcata acgttctggc taaagcatat ggttccacca acccgatcaa cgtggttcgt
     9601 gcaactattg atggcctgga aaatatgaat tctccagaaa tggtcgctgc caagcgtggt
     9661 aaatccgttg aagaaattct ggggaaataa accatggcaa agactattaa aattactcaa
     9721 acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa cgctgcttgg cctgggtctg
     9781 cgtcgtattg gtcacaccgt agagcgcgag gatactcctg ctattcgcgg tatgatcaac
     9841 gcggtttcct tcatggttaa agttgaggag taagagatgc gtttaaatac tctgtctccg
     9901 gccgaaggct ccaaaaaggc gggtaaacgc ttgggtcgtg gtatcggttc tggcctcggt
     9961 aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt ctggcggtgg cgtacgtcgc
    10021 ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct
    10081 cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc tggctaaagt agaaggcggt
    10141 gttgtagacc tgaacacgct gaaagcggct aacattatcg gtatccagat cgagttcgcg
    10201 aaagtgatcc tggctggcga agtaacgact ccggtaactg ttcgtggcct gcgtgttact
    10261 aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa tcgaggaata agtagcagat
    10321 ggctaaacaa ccgggattag attttcaaag tgccaaaggt ggcttaggcg agctgaaacg
    10381 cagactgctg tttgttatcg gtgcgctgat tgtgttccgt attggctctt ttattccgat
    10441 ccctggtatt gatgccgctg tacttgccaa actgcttgag caacagcgag gcaccatcat
    10501 tgagatgttt aacatgttct ctggtggtgc tctcagccgt gcttctatct ttgctctggg
    10561 gatcatgccg tatatttcgg cgtcgatcat tatccagctg ctgacggtgg ttcacccaac
    10621 gttggcggaa attaagaaag aaggggagtc tggtcgtcgt aagatcagcc agtacacccg
    10681 ctacggtact ctggtgctgg caatattcca gtcgatcggt attgctactg gtctgccgaa
    10741 tatgcctggt atgcaaggcc tggtgattaa cccgggcttt gcattctact tcaccgctgt
    10801 tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg ggcgaacaga ttactgaacg
    10861 aggtatcggc aacggtattt caatcattat cttcgtcggt attgtcgcgg gactcccgcc
    10921 agccattgcc catactatcg agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct
    10981 gttggttgca gtattagtat ttgcagtgac gttctttgtt gtatttgttg agcgtggtca
    11041 acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt cgtcgtgttt atgctgcaca
    11101 gagcacacat ttaccgctga aagtgaatat ggcgggggta atcccggcaa tcttcgcttc
    11161 cagtattatt ctgttcccgg cgaccatcgc gtcatggttc gggggcggta ctggttggaa
    11221 ctggctgaca acaatttcgc tgtatttgca gcctgggcaa ccgctttatg tgttactcta
    11281 tgcgtctgca atcatcttct tctgtttctt ctacacggcg ttggttttca acccgcgtga
    11341 aacagcagat aacctgaaga agtccggtgc atttgtacca ggaattcgtc cgggagagca
    11401 aacggcgaag tatatcgata aagtaatgac ccgcctgacc ctggttggtg cgctgtatat
    11461 tacctttatc tgcctgatcc cggagttcat gcgtgatgca atgaaagtac cgttctactt
    11521 cggtgggacc tcactgctta tcgttgttgt cgtgattatg gactttatgg ctcaagtgca
    11581 aactctgatg atgtccagtc agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta
    11641 cggccgataa ttggtcgccc gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg
    11701 tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt
    11761 gcagtgccga gccgaagcat aaacagcgcc aaggctgatt ttttcgcata tttttcttgc
    11821 aaagttgggt tgagctggct agattagcca gccaatcttt tgtatgtctg tgcgtttcca
    11881 tttgagtatc ctgaaaacgg gcttttcagc atggaacgta catattaaat agtaggagtg
    11941 catagtggcc cgtatagcag gcattaacat tcctgatcat aagcatgccg taatcgcatt
    12001 aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc atcctggctg cagcgggtat
    12061 cgctgaagat gttaagatca gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga
    12121 agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg
    12181 cctgatggat cttggttgct atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg
    12241 tcagcgtacc aagaccaacg cacgtacccg taagggtccg cgcaaaccga tcaagaaata
    12301 atcggggtga ttgaataatg gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac
    12361 aagtctctga cggcgtggct catatccatg cttctttcaa caacaccatc gtgactatca
    12421 ctgatcgtca gggtaacgcg ttgggttggg caacagccgg tggttccggt ttccgtggtt
    12481 ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga gcgttgcgct gacgccgtga
    12541 aagaatacgg catcaagaat ctggaagtta tggttaaagg tccgggtcca ggccgcgaat
    12601 ctactattcg tgctctgaac gccgcaggtt tccgcatcac taacattact gatgtgactc
    12661 cgatccctca taacggttgt cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc
    12721 aggtttgttg gagaaagaaa atggcaagat atttgggtcc taagctcaag ctgagccgtc
    12781 gtgagggcac cgacttattc cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa
    12841 ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg tctgtctgac tatggtgtgc
    12901 agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt cagttccgta
    12961 actactacaa agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc
    13021 tggaaggtcg tctggacaac gttgtatacc gtatgggctt cggtgccact cgtgcagaag
    13081 cacgtcagct ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt
    13141 cttatcaggt tagtccgaat gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc
    13201 gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa gccaacttgg ctggaagttg
    13261 atgctggcaa gatggaaggt acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg
    13321 acattaacga acacctgatc gtcgagcttt actccaagta aagcttagta ccaaagagag
    13381 gacacaatgc agggttctgt gacagagttt ctaaaaccgc gcctggttga tatcgagcaa
    13441 gtgagttcga cgcacgccaa ggtgaccctt gagcctttag agcgtggctt tggccatact
    13501 ctgggtaacg cactgcgccg tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag
    13561 gttgagattg atggtgtact acatgagtac agcaccaaag aaggcgttca ggaagatatc
    13621 ctggaaatcc tgctcaacct gaaagggctg gcggtgagag ttcagggcaa agatgaagtt
    13681 attcttacct tgaataaatc tggcattggc cctgtgactg cagccgatat cacccacgac
    13741 ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc acctgaccga tgagaacgcg
    13801 tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt atgtgccggc ttctacccga
    13861 attcattcgg aagaagatga gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc
    13921 cctgtggagc gtattgccta caatgttgaa gcagcgcgtg tagaacagcg taccgacctg
    13981 gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt
    14041 cgtgcggcaa ccattctggc tgaacaactg gaagctttcg ttgacttacg tgatgtacgt
    14101 cagcctgaag tgaaagaaga gaaaccagag ttcgatccga tcctgctgcg ccctgttgac
    14161 gatctggaat tgactgtccg ctctgctaac tgccttaaag cagaagctat ccactatatc
    14221 ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa
    14281 tctcttactg agattaaaga cgtgctggct tcccgtggac tgtctctggg catgcgcctg
    14341 gaaaactggc caccggcaag catcgctgac gagtaaccgg atcacaggtt aaggttttac
    14401 tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt caactgaacc gcaacagcag
    14461 ccatcgccag gctatgttcc gcaatatggc aggttcactg gttcgtcatg aaatcatcaa
    14521 gacgactctg cctaaagcga aagagctgcg ccgcgtagtt gagccgctga ttactcttgc
    14581 caagactgat agcgttgcta atcgtcgtct ggcatttgcc cgtactcgtg ataacgagat
    14641 cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg agccgtgccg gtggttacac
    14701 tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg ccgatggctt acatcgagct
    14761 ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa tctgaagcaa tgtaaaaaaa
    14821 cccgccccgg cgggtttttt tatacccgta gtatccccac ttatctacaa tagctgtact
    14881 ctttttgttc atcccctgga gtatttatgt ggttacttga ccagtgggca gagcgccata
    14941 tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc aggtagcggc gaaccattga
    15001 tactggatga tgattctcac gtgccaccgg aattacgtgc ggggtatcgc ttgctgaaga
    15061 atgccggttg cttaccgcca gaacttgagc aacggagaga agcaattcag cttctggata
    15121 ttctcaaagg tatccgtcac gatgatccgc aatatcaaga ggttagccgt cgattgtcat
    15181 tactggaatt gaagctgcga caagctggat tgagtaccga ttttttacgc ggcgattatg
    15241 ctgacaagtt gttggacaaa atcaacgata actagtggag tatgtatgta tcgcattggt
    15301 gagctggcaa aaatggcgga agtaacacct gacacgattc gttattacga aaaacagcag
    15361 atgatggagc atgaagtgcg tactgaaggc gggtttcgcc tgtataccga aagcgatctc
    15421 cagcgattga aatttatccg ccatgccaga caactaggtt tcagtctgga gtcgatccgc
    15481 gagttgctgt cgatccgcat cgatcctgaa caccatacct gtcaggagtc aaaaggcatt
    15541 gtgcaggaaa gattgcagga agtcgaagca cggatagccg agttgcagag tatgcagcgt
    15601 tccttgcaac gccttaacga tgcctgttgt gggaccgccc atagcagtgt ttattgctcg
    15661 attcttgaag ctcttgaaca aggggcgagt ggcgttaaga gtggctgttg attttttgca
    15721 ctgacaggat tacactcgcg ccgttaaata accaactgga gtttttatga gccgatatca
    15781 gcatactaaa gggcagataa aggataatgc gatagaagca ttactacatg atcccttatt
    15841 ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt tacatgcgaa aaggaaaaca
    15901 tggcaatcgg gggaactggg aggccagtgg caagaaagta aatcactttt ttaccactgg
    15961 tcttctgctt tcaggcgctt gttaagagcg gttattctgc tctttcagca aatcacgaat
    16021 ttctgccagt aatacttctt ctttagttgg tgcaggtgcg gcagctggtt cttctttttt
    16081 ccgattcagt ttgttgatta gcttaatcgc cataaagatg gcaaaggcca caatcagaaa
    16141 atcaaagacg ttttgaatga agacaccgta atgcatcaca acagcaggga tatccccctg
    16201 cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc ccgccaatta ataagcccag
    16261 aggtggcatg atgatatcgg caaccagtga agatacaatc ttcccgaatg ccgcaccgat
    16321 aatgacaccc accgccaaat ccaccacgtt cccgcgcatc gcaaattcgc gaaattcttt
    16381 aataatgctc atgttattct ccctatgaag ccgacaacca taagtctaac aaatgttaag
    16441 ccattttcct gccaggaatg aatttaatta atccttgata atcaaaggga aataataagg
    16501 cgtcattaga cgccttatta attacaagaa gaaagggctt ggctggaaga gtctttcgac
    16561 gtcggtaata aactttttat ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg
    16621 cagattgtca ttggcaatca tcacgtcgtt tccacgtacc accgctccaa taatcgttcc
    16681 tggcggtagc ttgatttcgt caatgactct gccgacaacg cgtgaggtgc tttcatcacc
    16741 gtgagcaacg gcttcaatag cttctgctac gccgcggcgc aatgaggaaa caccaacaat
    16801 atctgctttt cgcacatggc taagcaacgc agaaatagtt gcttgttgtg gtgaaattgc
    16861 aatatcgatc acgctcccct gaaccagatc cacataagcg cgacgctgga tcaataccat
    16921 cacctttttc gcacccatac gtttggcaag catggcggac atgatattgg cctcgtcatc
    16981 gttggtgacg gcaataaaca gatcaacttg atcgatatgt tcttcggcca gcagttcttg
    17041 atccgacgca tcaccaaaaa agacgatcgt attctgcaac ttttccgcca gttcggcagc
    17101 gcgctgctga ttacgttcga tgagtttaac gctgtaatct ttttccagac gacgcgccag
    17161 ccctgcaccg atattaccgc caccaaccag catgatccgc ttatacggtt tttccagtcg
    17221 ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg gcaataaaga acacttcatc
    17281 accagcttca acaatggtcg aaccttgtgg acgaatgggg cgatcgtggc ggaaaattgc
    17341 tgccacacga gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg cattaccaat
    17401 cagcgggccg ccataatagg ctttaactac agcgaggctg actttaccct cagcgaagtt
    17461 caccacctgc aatgcgcctg gatactcaat cagtcggtaa atattatcga taaccaactg
    17521 ctctggtgcg atcagatgat caatcggtac agcatctgaa tgaaatagct tatcggcatc
    17581 gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta ggggtgttga aaagtgagta
    17641 ggctacctgg caggcaacca tattggtttc atctgaactg gttacagcaa ccagcatatc
    17701 ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga gagccatgcc cctgcacgac
    17761 tcgcaggtca aatttatcct gcaaggtccg cagacgctca ccgttggtat cgacaacagt
    17821 aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct ggccggcacc
    17881 cagaatgata attttcatca gtctcgaccc gttatctcat cactttttga ttagcttagc
    17941 gtaaaagaag ccgtcgccct cttcggcacc aggcagattt tgtttacccg gttgctctgg
    18001 tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc aaaaaagctt taatctgcag
    18061 gctattctct tccggtaaca ccgaacaggt ggcatagacc agggttccac cggattttaa
    18121 atgcggccaa atggcgtcga gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg
    18181 atcgcgacgt aaccatttaa tatccggatg gcgacgaatc acccccgttg ctgaacaagg
    18241 cgcatctaat aaaatgcgat caaactgttg ctcgccacac cattgggaag ggtaacggcc
    18301 atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt aaattgtcgt gaacgcgaga
    18361 gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct tctggtgcca cctcaaggat
    18421 atgcgttgtt ttaccgccgg gggcggcaca aagatccaga atgtgttcac cgttttgtgg
    18481 cgcaagccag gtcatgcaac cttgtgctga tgcatcctga acggtaaccc atccgtcttc
    18541 aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc cgtacagcat cagggtaatc
    18601 cgcatgcggg aaacctttca ttcctgcttc atccaacaat gcaagccagc tgtcgcggga
    18661 atgatgcgta cgattaacgc gcagccacat tggcggacgc tggttattgg cttcgacgat
    18721 ggattgccac tgctctggat acgctttttg cagacgcttc agcaaccagg aaggatgcag
    18781 ataacgtgca tcactggtat taaactcggc taataactct tcttgctgac gctggaactg
    18841 gcgtaataca ccgttaatca accctttaag ttgcggacgc ttaattgcga tagcgccttc
    18901 aaccgtttca gccagcgcag catgaggtgg aatgcgggta taaagcagtt gatacaaacc
    18961 aaccataatc aggtaatgca cagtgcgctg tttgccggtc atcggacggg ccattaactt
    19021 attaatcagc cagtctaact gcgaaagcgt acgcagtacg ccaaagcaca actcttgaag
    19081 aagtgctttg tctttatcag aaactttttg ctggagcggt ggcagaatgt tgcttaatga
    19141 ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc atgctacgta aattacgttg
    19201 ttttttcata agtataaaaa tgcccggtaa gaccgggctt agaagagtgg actatcaggc
    19261 cagacggttg cccggaacaa accattcccg acgagaattc aggaggtctt gcgcgctcat
    19321 cgctttctta cccgcaggtt gtaacgagag caggttcagg atgccatcac cagtcgcaac
    19381 ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc
    19441 aatgaccgac gctttccaga ctttaaccgg ttgtccttca atttccagcc agctcattgg
    19501 ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct gccgaaagcg accagtcaat
    19561 acgcgcttct tctttactca acttctcggc gtaagtgaca agagtttcgt cctgaacttc
    19621 tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg attagccctt gtgggccaag
    19681 ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg ggcaggagag
    19741 cttatagagc atatcaccgg tgtctaaacc gacatccatt tgcataatgg tcacaccagt
    19801 ttctgcatca cccgcccata gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag
    19861 cagtgaacca tgaacgttga tacagccaag acgcggcatc tccagcactg ctttaggcag
    19921 aattaagcca taggcgacga cgaccataac atcagcctgc agatcggcga ccagttgctg
    19981 gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc agaccttttt cctcagccag
    20041 aactttaacc gggctgggca tcagtttttt accgcgtcct gccggtcggt ctggctgggt
    20101 gaacacgcca acgacgttat gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa
    20161 gtcaggtgta cccgcaaaaa taatacgtag tgattctgac acgttagttc ttatccttaa
    20221 gcccgggctt tcagacgatc tagtttttca actttctgac gaatacgttg ttgtttcagc
    20281 ggtgacagat aatccataaa cagtttgccg accaggtgat ccatctcatg ctgaatacag
    20341 atggctaaca gcccctctgc ttccagttca aatggtttac cgtcgcggtc aagggcgcga
    20401 attttaactt tctctgcgcg cggcactaaa gcacgttgtt cagggatcga caggcaacct
    20461 tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg gattgattaa caccagccgt
    20521 tcgtcacggt tttccgaaac atcaataaca atgatacgtt gatggatatc aacctgggtt
    20581 gccgccaggc caataccttc ttctgcgtac atcgtctcga acatatcatc gacgatacgc
    20641 tgtatttctg cattcacttc ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga
    20701 atatgtaaca cttgcaaaac tgacataaat ctccagagat gtgttcaggg gttagaaaga
    20761 ttatttcttc tattctagac aaatccccct ctgattgaca gcatcactga ccaatcgcaa
    20821 agattgctaa ggctgcttat ggcagggaga taaggatggt cgatacagaa atttggctgc
    20881 gtttaatgag tatcagcagc ttgtacggcg atgatatggt ccgtatagct cactggctgg
    20941 caaaacagtc gcaaattgat gcggttggat tgcagcaaac agggcttaca ttgcggcagg
    21001 cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag ctcactttgt tggttggagc
    21061 aacccaacca tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttctggtga
    21121 cgacagatta ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc
    21181 agcttgccgt agtggggagt cgggcgcatt catggtatgg cgagcgatgg ggacggttat
    21241 tttgcgaaac tctggcgacg cgtggagtga caattacgag tggactggcg cgtggaatcg
    21301 atggtgtggc gcataaagct gccttacagg taaatggcgt cagcattgct gtattgggga
    21361 atggacttaa taccattcat ccccgccgcc atgcccgact ggctgccagt ctgcttgaac
    21421 atggcggagc tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc
    21481 cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt
    21541 tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga gcagaggcgt gaagtttttg
    21601 ccttgccagg tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag
    21661 gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact
    21721 ggttgccaga cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc
    21781 catttcctga gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg
    21841 aacgtgccgg ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag
    21901 gatggatcgc agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac
    21961 gtactaatgt atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa
    22021 gacaaacttg aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc
    22081 ctgctatggc tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg
    22141 gcctcggatc ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc
    22201 agctgccgtg ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt
    22261 gaaatggtga tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg
    22321 aaatgggtga tcctgatggt gttgttcaac atcccggggt gcgaaaatgc gtaccagcaa
    22381 atggaagaat tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc
    22441 agcataagtt gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc
    22501 aaagtgcggg gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc
    22561 acagtatccg gcgtgtgact acgtccgtct tctgaaatct tcagcggatg gacatattgt
    22621 caaagttctg gaggggcagg tttgccctgc atgtggcgca aatctggtat tacgccaggg
    22681 acgctttggt atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat
    22741 cgataaaccg gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca
    22801 gcgccgctcc cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt
    22861 tgccattaac ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat
    22921 cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc
    22981 ggtttcggcg gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga
    23041 tagatgtcct caatgaagaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgttg
    23101 ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg
    23161 ttgataaggg gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg
    23221 acaccatgct gactgatgcg cagcgtgaaa ccattttttc ccgctggcca ggtcctgtca
    23281 cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc
    23341 ttgctgtacg agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac
    23401 cgctggtttc taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag
    23461 ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa
    23521 atccttcaga aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg
    23581 gaaacctatg ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag
    23641 caatttgctc agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat
    23701 gatttcatca acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg
    23761 gtgcctttta aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg
    23821 gctggtgcag ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc
    23881 gatggtgtag gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt
    23941 attctgctta tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttcccta
    24001 gactgtgcgg tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg
    24061 tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt
    24121 gatctcatta ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca
    24181 tcgctcattc atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct
    24241 tttctggcat ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg
    24301 gtggcacagg cggctcatgc cttccttctt tggcatggcg ttctgcctga tgtagaaccg
    24361 gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca ggccatccag tttccggata
    24421 gggaagagtg ggacgagaat aaaagatgtg tatgttttcc tgctctcgtg aatggtatgc
    24481 aactgacatg tgcgatctct ggcgagagtc tggcgtatcg ctttactgga ggtacgccag
    24541 aacagtggtt agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact
    24601 taattcagga acaaagtgaa gatgatcaag gttgggtctg gttaccttga tccagatatt
    24661 cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa cccagccttc tcttcatcac
    24721 ttaacgggcg gatctgtttg acgggactac cgagatacag atatccgctc tccagccgtt
    24781 tattttgtgg gaccaggcta cccgcaccaa tcatcacatc atcttctact attgcgccat
    24841 caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca
    24901 tcaccttgtg accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt
    24961 acgaggattt atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga
    25021 tctgtacata atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag
    25081 ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat cattacgcgc tggccgattt
    25141 gtggaaaaag atcgcggtat gggcgtaaaa catcagacat actcacctca acaataaatg
    25201 atttcctaat gactttgggt gcattattag ccttgtgcaa gtcttttagt atgcaaaaaa
    25261 gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa acaacaaaca gaaaaaaaga
    25321 tcaaaaaagt acttgtgcaa aaaattggga tccctataat gcgcctccgt tgagacgaca
    25381 acgtgaaaca cttcacagga tggtcggaac aacgaagaga aaaaattctg aaattcaggg
    25441 ttgactctga aagaggaaag cgtaatatac gccacctcgc gacagtgagc tgaaag
//
LOCUS       DAJTXQ010000051        25426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000051 DAJTXQ010000000
VERSION     DAJTXQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.051"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            124..447
                     /gene="soxS"
                     /locus_tag="OO515_002035"
     CDS             124..447
                     /gene="soxS"
                     /locus_tag="OO515_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6575975.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="OO515_002036"
     CDS             complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="OO515_002036"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6575976.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2466..2746
                     /locus_tag="OO515_002037"
                     /pseudo
     CDS             2466..2746
                     /locus_tag="OO515_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="OO515_002038"
     CDS             complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="OO515_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6575977.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3635..6457
                     /gene="uvrA"
                     /locus_tag="OO515_002039"
     CDS             3635..6457
                     /gene="uvrA"
                     /locus_tag="OO515_002039"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6575978.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6492..6848)
                     /locus_tag="OO515_002040"
     CDS             complement(6492..6848)
                     /locus_tag="OO515_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6575979.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6852..7268)
                     /locus_tag="OO515_002041"
     CDS             complement(6852..7268)
                     /locus_tag="OO515_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6575980.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="OO515_002042"
     CDS             complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="OO515_002042"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6575981.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8494..8716
                     /locus_tag="OO515_002043"
                     /pseudo
     CDS             8494..8716
                     /locus_tag="OO515_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="OO515_002044"
     CDS             complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="OO515_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6575982.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10666..11745)
                     /gene="alr"
                     /locus_tag="OO515_002045"
     CDS             complement(10666..11745)
                     /gene="alr"
                     /locus_tag="OO515_002045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6575983.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="OO515_002046"
     CDS             complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="OO515_002046"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6575984.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13296..14279
                     /locus_tag="OO515_002047"
     CDS             13296..14279
                     /locus_tag="OO515_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6575985.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="OO515_002048"
     CDS             complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="OO515_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6575986.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="OO515_002049"
     CDS             complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="OO515_002049"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6575987.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16221..17206)
                     /locus_tag="OO515_002050"
                     /pseudo
     CDS             complement(16221..17206)
                     /locus_tag="OO515_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17524..18039
                     /gene="zur"
                     /locus_tag="OO515_002051"
     CDS             17524..18039
                     /gene="zur"
                     /locus_tag="OO515_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6575988.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18081..18290)
                     /locus_tag="OO515_002052"
     CDS             complement(18081..18290)
                     /locus_tag="OO515_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6575989.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="OO515_002053"
     CDS             complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="OO515_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6575990.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="OO515_002054"
     CDS             complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="OO515_002054"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6575991.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="OO515_002055"
     CDS             complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="OO515_002055"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6575992.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21061..23484
                     /gene="plsB"
                     /locus_tag="OO515_002056"
     CDS             21061..23484
                     /gene="plsB"
                     /locus_tag="OO515_002056"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6575993.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="OO515_002057"
     CDS             complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="OO515_002057"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6575994.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24523..25020)
                     /gene="ubiC"
                     /locus_tag="OO515_002058"
     CDS             complement(24523..25020)
                     /gene="ubiC"
                     /locus_tag="OO515_002058"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6575995.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25243..>25426)
                     /locus_tag="OO515_002059"
     CDS             complement(25243..>25426)
                     /locus_tag="OO515_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6575996.1"
                     /translation="LNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALFP
                     IFTASWRATRIKNIGI"
BASE COUNT         6578 a         6542 c         6089 g         6217 t
ORIGIN      
        1 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
       61 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
      121 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
      181 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
      241 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
      301 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
      361 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
      421 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
      481 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
      541 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
      601 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
      661 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
      721 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
      781 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
      841 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
      901 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
      961 gaaacatagc gatcggcatg tgttgccaag tacgcgccca gatcgcggaa gacattatca
     1021 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
     1081 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
     1141 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
     1201 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
     1261 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
     1321 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
     1381 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
     1441 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
     1501 gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact ccagaagaga
     1561 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
     1621 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
     1681 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
     1741 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
     1801 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
     1861 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
     1921 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
     1981 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
     2041 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
     2101 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
     2161 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
     2221 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
     2281 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagc tcacaaaatt
     2341 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     2401 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     2461 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcagctt cttagtgccg
     2521 taatgatgtt tctggccagc acatcaacat ccgttttcgt cggtctgatt atgtcgggct
     2581 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
     2641 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
     2701 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagcctc
     2761 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
     2821 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
     2881 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
     2941 ctgattgcca ccctgcggct gctgaggctg cccccaaccg ccctgcggct gaccaccacc
     3001 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
     3061 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
     3121 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
     3181 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
     3241 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
     3301 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
     3361 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
     3421 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
     3481 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
     3541 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
     3601 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
     3661 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
     3721 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     3781 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     3841 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     3901 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     3961 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     4021 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     4081 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     4141 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     4201 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     4261 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     4321 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     4381 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc
     4441 gtttaacaac ccggcaggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt
     4501 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     4561 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
     4621 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     4681 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     4741 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
     4801 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     4861 cgaaggaacg cgtctgcgtc gggaagcgcg ccacgtgtat gtcgagaata cgccgctgcc
     4921 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
     4981 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     5041 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     5101 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     5161 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     5221 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     5281 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     5341 tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg
     5401 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     5461 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     5521 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     5581 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     5641 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     5701 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     5761 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     5821 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     5881 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     5941 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     6001 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     6061 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     6121 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     6181 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     6241 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     6301 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     6361 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     6421 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     6481 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     6541 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     6601 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     6661 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     6721 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     6781 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
     6841 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     6901 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     6961 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     7021 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     7081 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     7141 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     7201 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     7261 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     7321 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     7381 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     7441 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     7501 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     7561 attttgtatt ttgccctggt ttatcgcccg caaagattac cggattcatg ttggtggcag
     7621 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac
     7681 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     7741 cctcttttgg aatgctgaat tcatcccagc cattattcat tttttcccag aacacaggat
     7801 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aaccccggac
     7861 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     7921 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     7981 caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     8041 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     8101 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     8161 ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     8221 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     8281 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     8341 aatttgaaag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     8401 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     8461 actaatttta ttactcccga tggagatgat gatatgaata tatcatatgt gaatagcaac
     8521 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagacat ctcttatgcc
     8581 aaagattttt cttatgctaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     8641 aaagattttt gtagacctgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     8701 gcatttatcg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     8761 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     8821 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     8881 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     8941 tatattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     9001 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaaggaattc
     9061 cagataattg ggataaactt aacaataaaa gaactgacaa cgttttatta tctctcacgt
     9121 agaacaacgg catcaaaaaa atcatctaca aagaaatagt gaaagcgacg acagtttaat
     9181 ttcactgcag gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc
     9241 ctttgccaca cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc
     9301 gatgagatag acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt
     9361 ataactgaac atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt
     9421 gcttaatacc ttcaccaatt cctgacgcat tgccagaatg cgagtacgca tctcttctac
     9481 ttccgccagc cagctggctt tcaatgcctc gtcattcagc actgcagcca ccacctgcgc
     9541 accaaaattc ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac
     9601 gcggccagcg gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta
     9661 aagggagaaa attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat
     9721 ggcgcgaata gcgtaggcat cctcttccat accggcacta aatccttgat aggcaatatc
     9781 gaggaatggg ataagctcgc gggctttgag aatttcaatc accgcatccc actgatcatt
     9841 agtgagatcg gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc
     9901 aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc acgctgttag tcgcttcgtc
     9961 ataccagggg taagtactca cttcgaatcc agccccggcg aatattgcta cgtggttttc
    10021 ccaggtagga tcgctgaccc agacgcctga ttccgggaag tagcgtttca ggaaatccgc
    10081 gcccactttc aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg
    10141 cagtaccgga tggtccgcac caaacagcag cggcgcaatg gcatggcgat agctgttaag
    10201 cccttccatc ggtaaataaa gcgaagcgcc atggggctgc gcattcaggc gcgcttccgc
    10261 ctccgccacg gctttcagtt gtggaataat tccgtcttcg ttgtagtaca gaccgatact
    10321 taaattcact ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg
    10381 atcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag
    10441 gtggttaaaa cacaataaac cggaagaagg cgaagatcga gtggatgttc aggagcgaac
    10501 ggcaattagc aacagagtga gactcatgac aaacgaacat ccgccagagg cacgaccttt
    10561 ataagagcat gaaagaacat caacttattg aatttttagg attttattgg ccggataagg
    10621 cattcacgcc gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt
    10681 catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa gcgctcactt ttgtcatttc
    10741 agcgatacgt tctacgggca aaccttcgcc ccataaaatg accggatccc cggctttgtc
    10801 ctgcgcctgt ggacctaagt ctacgcagat catatccatc gccacgcgtc cgacaatcgg
    10861 tacttcgcga ccgttcacca gcactggcgt accggatgga gcggcgcgcg gataaccatc
    10921 gccatagccc atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc aggttccacc
    10981 ataaccaaca ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga
    11041 catcactggc tgacagccaa aatcggcacc gatggagcga tcttccagcg gcgagacgcc
    11101 ataaagaatg atgcccgggc gcacccagtc aaaatgcgat tgtggccaca gcagaatgcc
    11161 acccgacgcg gcaatggaac gttgaccagg tttgccttcg caaaaggtat taaagatagc
    11221 gagttgtttc tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat
    11281 attcaccggc tgacgaacgt ttttgcactg tgtcaggcga tgataaaacg cctcagcctg
    11341 ttccggcctt acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg
    11401 ctcgtccagg ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg
    11461 aaaatgttgc gcagaaatcg tcggcagatc tctggcatca aaaaagcctt cgagtaacag
    11521 tacaggtttg gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc
    11581 aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt
    11641 cgctttcacc accgcaacca ttttactggc aggggccagt tcacgaagac gttgcaggtt
    11701 gtgtcgcaga gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata
    11761 agtgtttgct ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc
    11821 ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg
    11881 ggccgttacg ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt
    11941 gatacacctc atcacgatag ataaacataa tcaagtccgc atcctgctcg atagagccag
    12001 attcacgcag gtcggagttg accgggcgtt tgtcggcacg ttgttccaga gaacggttca
    12061 actgggacag cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcgag
    12121 agatttctgc aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca
    12181 ggtagtcgat catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg
    12241 aacgcacttc cgttggcgtc aggccggagg agtcatcgat atagatattg cgtttttcga
    12301 gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc atcatcgagc tgcccggtac
    12361 ggattttagt ctggtcaacg cgcgacagcg acgccagaga acgcatcata atctgttctg
    12421 aaggcatctc cagcgagaag ataagtactg gtttatcctg caacatcgcc gcgttttcga
    12481 cgaggttcat cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg atgatcaaat
    12541 ccgacggctg caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttactccgg
    12601 taacgccatc gtgtggctgc tgaaacaact gctcaatacg cgccacggtt gcgtcgagca
    12661 catcggcgat gttcttcggc ccttcgtctt tgtttgcacg actttcggca attttaaaga
    12721 cgcgggattc agcaaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg
    12781 cttcagcaat ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga
    12841 tgtcagcata agcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat
    12901 aagcaaaacc accgacgcta tcgagttgcc cctggcgttc cagcgattcc gcaagggtaa
    12961 tcagatcgat aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatggcggt
    13021 gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc tacatcgtcc cagcgttcgt
    13081 tatccagcat taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt
    13141 tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt
    13201 tatttcctgc catagtgaat ggagttaccg agataaagaa tgggtcgaaa ctttaccata
    13261 tgaagcagac cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca
    13321 agcacggtgg cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcagaga
    13381 atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg tatatccgca
    13441 gcggccttta cccgccgcca tcactaccca gcggattagg taccgaagcg gcaggcatcg
    13501 tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta gtctatgcgc
    13561 agtcggcgtt aggcgcttac agctctgtgc ataacattaa tgcggataaa gcggcgattc
    13621 tgcctgcggc aatttctttt gagcaagctg cggcatcctt cctgaaaggc ttaacggttt
    13681 attatctgct gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag
    13741 cggctggcgg cgttggctta attgcctgcc agtgggcaaa agccctgggc gcgaaactta
    13801 tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc gcgtggcagg
    13861 ttattaacta tcgtgaagag gatctggtcg agcggttaaa agagatcacc ggcggtaaga
    13921 aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc
    13981 tgcaacgccg cggcttaatg gtcagttttg gcaactcatc aggtgcggtt accggtgtga
    14041 acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct
    14101 atatcaccac gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca
    14161 gcggtgtgat taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc
    14221 gtgcgcatga gattctggaa agccgggcga cgcaaggttc cagcctgttg attccataaa
    14281 agaaataggg cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta
    14341 cagcacgatg aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt
    14401 gttctaagga taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg
    14461 tcataacgct gatatttcgg cacttttggt gctttaatcg ccttaataac ccacaccacc
    14521 gcaatcgcca gtagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg
    14581 aacataatgg ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg
    14641 accatcagca tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa
    14701 atatgaaatc tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca
    14761 gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt gactactttt tggtgaaaag
    14821 ttaacgctta tccgccacca gtttgagcgc gtgttccagc acattaatgt ctgcacccgc
    14881 tttatgggca ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa
    14941 caagcccaac atatgccggg taatatggcc gagatacgtc ccctggctga gttcacgctc
    15001 aatgtacgga tacatggcgc gcactaccgc caccggatcg gcatcggtat ccgaggaacc
    15061 aaagatctcc cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac
    15121 catcacgcca tccatatgtt gcaggtgtgc tttggcctct tccagcgact tgataccacc
    15181 gttaatcgac attgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc
    15241 gagcggcggg atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc
    15301 gtggatgata aacatctcac actcgccttt gccggaaacg gtgttgatga aatcgcagag
    15361 aaattcatag ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca ctggaatcga
    15421 caccacatcg cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca
    15481 cgcaccaaac atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc
    15541 atcatatcca cgcgcttctg ccagcttcgc acactgtgcc agcgccgccg gatcgctacc
    15601 gccgagttgc aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc
    15661 gtgaataatc gcccctgtgg tcaccatttc ggtatacagc aacgtattgc gggaaagcag
    15721 acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cgctaaaccg
    15781 accactccag tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat
    15841 ttcagaatta ccgtgcattt tcaaatgtag agattatttt atcgatatat catggggtta
    15901 tgttattcag catcactatt caggaggctc aatagcgggg tactatacca taacaacagg
    15961 aagcgcctgt ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga
    16021 agtgatggaa ataatcttat tagccgttaa ctttgttaag gccaatgata aacaatccag
    16081 gttcgacgat aaataaaaaa tcacacatta aactctggtg atatatctcc ctgctaatag
    16141 cattgataga gaaaaaagaa cccaataaat attgggtcct tttatataat gccttccata
    16201 ctatcgaaga acttcacata ttatttctcc gatttaaccc cgaacaaatc ataaattaat
    16261 ttagaagtgt ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa cgacttcgcg
    16321 aagctaatta attgtggtgc agcatctcgc attttgctta aaatatcagg atcgagcgtt
    16381 ccttcattca ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca
    16441 tcagccagac gcttcatctc tcccaggtac ttcttcatat cacgttgaga taatttttca
    16501 aactgagctt tcaggtagct ctcattattt tcattaatat acactgtttc tgataaatct
    16561 gcgtaggcac tagaatatga aagtgaaatt aacagtgaag caaataattt taagctgttg
    16621 tcatcatgtt cctggcaggc attaacaaac gttaaaaaac cagaacctat tgcttgaaaa
    16681 tgtatattcg ctaaaggttc attatcctta gattcctcat aaaatagctg aaccgtggag
    16741 ggaaggccat tcttagtttc tacatcaaaa gtacattgtt caatgggagt cgcatcttta
    16801 ttatccccag gattacatat actattaatc gcgaaatgaa aaatagcccg gtcaacaact
    16861 gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc aatcgtcaat atacttattg
    16921 acccacattt tgagatcatt tatttgctca attaaaaaca cattttttta cgtgcatgcc
    16981 ataaattatg cgtgattcgt tgaataaaca aatctatttt tgcaataatt atatcccgat
    17041 gacgcagcgc tgcataatca atagatttac catatatatt atttaattta cctttgattg
    17101 aacctctatt atctaattta tctttgattg catcttcatt atcgaattga tttgaattga
    17161 gtgttttatt aaagtatcca gttgcaccgg gtattatctt caacatgatt catctacctt
    17221 ttttgtcgta ccgtgtcctc cttataattc ttttgccatc tggtcactat caaaaatcgc
    17281 tcataatttc tgaactacag gtatgcgttt aaacccagtt aaataaatag cgagttaact
    17341 tgaagcaatt tatttaaaaa tgaattatta attattatcg tgttgataat ggtacgacaa
    17401 ggcaacataa caccttacct acaaaacaga aaatcaacat tacggcaaca ataagggttc
    17461 tcggtgtttt tcctggaaca tggtaaagta aggacattct taacccccac tttgaggtgc
    17521 ccgatggaaa agaccacaac gcaggagtta ttagcgcagg ctgaaaaaat ctgcgcgcag
    17581 cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc gcctgatgag tctgcaggat
    17641 ggcgctatca gcgcttatga tctgcttgat ttactgcgcg aagctgaacc gcaagccaag
    17701 ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc aaggttttgt gcataaggtg
    17761 gaatccacca acagttatgt gctctgtcat ctgttcgacc agcccacgca tacgtcagcc
    17821 atgtttatat gcgatcgctg cggcgcggtg aaagaagagt gtgcagaagg cgtggaagac
    17881 attatgcata cgctggcggc aaaaatgggg tttgccctgc ggcataatgt gattgaagca
    17941 catgggctct gtgcggcatg cgtggaagtg gaagcgtgtc gtcatcctga acagtgccag
    18001 catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaag agggcgtaca tccttgtaca
    18061 cgtcgggcag gagggattaa ttaccagcga tattcattgc gggtttccca atccacgacc
    18121 tctttttctg cctgatcttt ctgataacca taacgttcct ggattttacc gaccagttga
    18181 tcacgtttac cttcaatgat cgtcatatca tcatcggtca gtttgcccca ttgctctttc
    18241 actttacctt taaactgttt ccagttaccg ccggcttcat ctttattcat aatcaagacc
    18301 tcatcgttag gttgtgaatg agagtacgtt cacttttctt ctgaacgtga gattaagtgt
    18361 agtcagcaat ttggcttagg ataatttatt cagaattttt aaccgtcacg ttgcggcaaa
    18421 ccaggtaccg ttacgccagt gacgccgcca gatagccgcc agagaaagcc cgcgcaacgc
    18481 cagaaagacg gttagtgcca gccacaaacc atgattaccc agccacggca gcgtaaggag
    18541 cgtcagcgca aaacctgcgg cggccaccgc catactgtta cgcatttcgg cggcacgcgt
    18601 tgcgcctata aacatgccgt ccagcagata acaccagacg ccaaccaacg gcaaaatcac
    18661 ctgccagata agatagcggt cagccagctg ctgaatctgg gttaacgacg tcagcaacgc
    18721 aatgatgtgt tcccctgcca gcaaataaac caccgaaaat agtaacgcta cgatccccga
    18781 ctggcggcac gctgcccgcc agacatccag caactggcta ccgtcgcgcg caccgtatgc
    18841 ctgaccggaa tgtgcttcaa ccgcgtaggc aaaaccatcc agcgcatagg cggtaaaggt
    18901 gagtagcgtc atcagaaccg cgtttacagc gataatgtca ctccccagtc gcgcgccaag
    18961 tacggtgatc gcgccgaaac agagttgcaa caacagcgaa cgcagcatga tatcgcggtt
    19021 aagcgccagc aagcgacgga agtttcctcg ccaggcagtt ttcgacattt cgccggaaat
    19081 tccgcgtagt ttgaggattt tacgcaccat tagcagacca atcagcaatg ttgcatattc
    19141 cgcaataacc gtcgccagag ccgcgccctg cacgttcata tgcagcccca tcactagcca
    19201 gacatccagc acaatgttga ggatattgcc gaccactaac aaaattactg gcgcacgggc
    19261 atattgcacg ccgagtaacc aaccgagtaa taccagattc gccagcgacg ccggtgcgct
    19321 taaccagcgg atttcaagaa agcgccgcgc ctgttccagg actgcttcac taccgccaac
    19381 aatatgcaga gccagatcga taatcggtgt acgcagcagc gcaattaacg ccccagcccc
    19441 caacgccaac agcaacggtt gcaccagcgc acgggctaat gcctgaggat ttttggcacc
    19501 ataagcctgc gcagtcagcc cggtggtgct catgcgtaaa aacagcaaca gcataaagag
    19561 aaagctggtc gccgttgcac caaccgccac gccgcccaaa taaaccgggc tatcaagatg
    19621 accaattacc gccgtatcga ccagtcccag caacggaacg gtgatattgg agaaaatcat
    19681 gggtaaggcg agatgccaga gtgctttatc agatgaagtg aggaatgcca tgcagacaag
    19741 cctgatgaag agagatgaaa aacaaaccgc gacgcctggc ggcatcgcgg tctcagagat
    19801 atgttacagc cagtcgccgt tgcgaataac cccaaccgcc agcccttcaa tggtgaagct
    19861 ctgctgacga aggtcaacga caattggttt aaactcgcta ttttctggca acagttcgac
    19921 tttattaccc tgttttttca ggcgcttaac ggtaacttcg tcatcaatac gtgcgacaac
    19981 gacctgaccg ttacgtacat cctgagtttt atgcactgcc agcaagtcac catccataat
    20041 gccgatatct ttcatcgaca tcccgctgac gcgcagcagg aaatcagcat tcggcttgaa
    20101 caaggaagga tcgacctgat aatgaccttc aatatgctgc tgcgccagaa gcggttcacc
    20161 ggcagccaca cgacctacca gcggcaaccc ttcttcctct tcctgcaaca gacgaatccc
    20221 gcgtgatgcg ccggaaacaa tttcaataac gcctttgcgt gccagcgcct tcagatgttc
    20281 ttcagccgcg tttggggaac ggaaccccaa acgctgcgcg atttccgcac gcgtcggcgg
    20341 catacctgtc tggctgatgt gatcacggat gagatcaaac acctcttgtt gcctggccgt
    20401 taacgctttc attccgcccc ctgggtgtat atacagttat gctgtgagta tatacagcaa
    20461 aaggcgattt tggaaccata aactgcacaa taaaccagag atttatcgaa atctggaagg
    20521 gttatccaaa atgcgaccat aacaggatgc accaggttat cacggcgacg ataatcgcaa
    20581 tcagcaccgc ggcggatccc atatctttag cgcgtccgga aagctcatgg tattcagagc
    20641 caattcggtc aaccactgct tcgatggcgc tattgagaat ttccacaatc atcaccagca
    20701 tcacggagct gataagcagc acgcgggtaa ttgcgtccac atccagccag caggcgatga
    20761 ccaccgccaa caataccgct acgccttcct gacggaatgc cgcttcgttg atccatgcag
    20821 cgcgtaaacc tttccaggaa tagccagcag ctttgataat tcgggtgaat ccagtggtat
    20881 tattggccat tgaaagaacc tttttacatt atgagcgtca atatcagtgt accgttaaat
    20941 ccgatgattc gggtttgcgt aacgccgcgc gaaacatgag cggataccac agaatttccc
    21001 atgactttct gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt
    21061 atgtccggct ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta
    21121 aaaagcaagt ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca
    21181 attatgtacg ttttaccgta caactcgaaa gcagatttgc tgacgttacg cgcccagtgt
    21241 ctggctcatg acttgcctga cccgttagag ccgctagaaa tcgacggcac gctactgccg
    21301 cgctatgtgt tcattcacgg cgggccgcgt gtattcacct attacacgcc gaaagaagag
    21361 tctattaagc tgttccacga ctatctcgat ttgcaccgta gcaacccaaa tctggatgtg
    21421 cagatggtgc cagtatcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagta
    21481 aacccgccgc tgcgtatgct taacggcgta cagaaatttt tcgccgtact gtggctcggt
    21541 cgcgacagtt ttgtgcgttt ctcgccgtca gtttcgctgc gccgtatggc ggatgaacac
    21601 ggcacggata aaactatcgc acagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt
    21661 caacgtctgg ctgccgtagg cccacgtctg cctgctcgtc aggatctgtt taataagctg
    21721 ctcgcctccc gcgccattgc caaagcggta gaagatgaag cgcgcagcaa aaaaatctcc
    21781 catgaaaaag cgcagcagaa cgcgattgcg ctgatggaag agattgcggc gaatttctct
    21841 tacgagatga tccgcctgac tgaccgcatt ctgggcttca cctggaaccg actttatcag
    21901 ggcatcaacg tccataacgc cgagcgcgtt cgccagctgg cccacgacgg tcatgagctg
    21961 gtgtatgtgc cttgccaccg cagtcacatg gactacctgc tactttctta cgtgctgtac
    22021 caccaggggc tggtgccgcc gcatatcgcc gccgggatca acctgaactt ctggccggcc
    22081 gggccgattt tccgccgtct gggggcgttc tttattcgcc gtacttttaa aggcaataaa
    22141 ctttattcca ccgttttccg tgagtatctc ggcgaactgt tcagccgtgg ttattccgtc
    22201 gagtacttcg tggaaggcgg tcgttcccgc acggggcgtt tgctggatcc gaaaaccggt
    22261 acgctgtcga tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt
    22321 ccgatctata tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc
    22381 ggcgcgacga aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt
    22441 aatctcggtc agggttacgt caacttcggt gaaccaatgc cgttgatgac ctaccttaac
    22501 cagcacgtac cagactggcg tgaatctatc gatcccatcg aagcggtgcg tccggcctgg
    22561 ttaacgccga cggtcaataa tattgctgcc gatctgatgg tacgcattaa caacgcaggc
    22621 gcggcaaacg ccatgaacct gtgttgtact gcgcttctgg catcacgtca gcgctcactc
    22681 acccgcgagc agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtacct
    22741 tactccacgg actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg
    22801 caaatgaaca agtttgaagt cgagaaagac actatcggcg acatcatcat tctgccgcgc
    22861 gagcaagcgg tgctgatgac ctactatcgc aacaacattg cgcatatgtt ggtgctgcct
    22921 tcgctgatgg cggcaattgt cacccagcat cgccacatct cccgcgacgt attgatggag
    22981 cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt tcctgcgctg ggatcgcgac
    23041 gagttgccgg acgttattga tgcgctggca aatgagatgc aacgtcaggg gctgattacc
    23101 ctgcaagatg atgagttgca tatcaacccg gcgcattctc gccccctaca gctgctggcc
    23161 gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac
    23221 ccgtcgatca accgcggtac gctggagaaa gagagccgca ccgttgcgca acgtctctcc
    23281 gtgctgcacg gcatcaacgc gccggagttc ttcgacaagg cggtgttcag ttctctggtg
    23341 ctgacgctgc gtgatgaagg gtatatcagc gatagcggcg acgccgaacc ggcagaaacg
    23401 atgaaggttt atcagttgct ggcggagttg attacatcag acgtgcgttt gacgattgag
    23461 agtgcgacgc agggcgaagg gtaatcagag agaattgccg gatgcggcga aaacgcctta
    23521 tccggccttc cataatctgc aaattcaata aattgcgatg tgttgtgtag gcctgagaag
    23581 cgcagcgcat caggcaaccc agaagaaagc cggatgatca tccggctttt ttacatcatc
    23641 agaaatgcca gtaactcatt gccagtccta aaaacagtac cagaccaaca tagttattat
    23701 tcataaatgc tttaaagcag gcttcacgct cgcggttggc aatcagtttt tgttgataaa
    23761 caaacagcgc gccagccacc agaattgacc agtaatatcc ccagcctaag ccatttaact
    23821 caccgatgat cgccatcagt gccagtacgc caatctgcaa aataccaata atcaatttat
    23881 cgtattggcc gaacaggatt gcggtggatt taataccaat cttcacatca tcgtcacggt
    23941 caaccattgc atactgcgtg tcgtaagcca ccgcccagag aatattggcg aggaacatta
    24001 accagcaact caatggcacc gactcactca cagcggcaaa agccattgga atcgaccagc
    24061 caaacgccgc gcccagcacc acttgcggga gatgggtata ccgcttcata aacgggtaca
    24121 cccacgccaa cgccagagcg gcaatcgaca acagaatggt catcgtattc agcgtcagca
    24181 ccagtaaaaa tgaaatcaat accagcacta caaacagcgc gcgcgcctct ttctccgtta
    24241 ccgcgccgct gggaagcggt cggttcgccg tgcgcttaac atgaccatca aacttgcggt
    24301 cagcataatc attcaccaca catccggcag cgcgcatcag ccagacaccc gcgacaaaca
    24361 ccgccaggat ccagagctgg ggaacgcccg gtgtcgccac ccacaacgcc cataatgttg
    24421 gccagagcag cagtaacgcg ccaattggct tatccgtacg cattaagcga tgaaacgcca
    24481 gcagcttatt ctgcgtcaga ctccactcca tattttttcc tcttagtaca acggtgacgc
    24541 cggtaaaaac agttctgtta gcaacagcgg tttaccgctt aatcgcaggc gggaacgtcg
    24601 cccccacagt ccggcatcac ggcctatctc aataaagtcc cgggttaatg tcgatgatgt
    24661 gaacagatag cgtcctagcg gcgttttacc caatttttgt aacgccagct ccggcccgct
    24721 taacgttgac acaggaacga cggtacgacc ggcaagccac ggttcaccat cggcacataa
    24781 caaaatttca cgtaaccagt aacgagactc tttcggcagc agcggcagtt cttcggggat
    24841 ttcattctgc tcgacaaacc cttcgcggat catcgtcacg ctcaccgttt ttccctgctg
    24901 ttcaaaacgt tttgtcatgg aatcttccag caacagccag tcgagcagtt gcgaatccag
    24961 ggcagggatc tctttaaaat agcgcagcgc acgcagttgc gttaacgcgg ggtgtgacat
    25021 gccgaactct ccgttacata acgtaaaggc attgtatcgc agaaaagtgt ttacgggaga
    25081 tgtaaagcaa attaagtgat ctaatgcgca acaaaaacgc aacaatcgca tgaagtggta
    25141 aaacgctgtt gaaccttacg ctttgccgat gcccgtgctg gcgagggcaa ccgtaggggc
    25201 atcaccgccc atgatgccag gaacggtgat gttttccttt acttaaatac ctatattctt
    25261 tatacgagta gctcgccaac tcgcagtaaa aattgggaaa agcgcatatt taccatgaga
    25321 tttggagcta aaaagtgttt cctgagtaag attaataggc aacatcttat gtctggattt
    25381 atagatacac gtggaattcc aattggtatc ttttgtagga ttcaac
//
LOCUS       DAJTXQ010000052        25130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000052 DAJTXQ010000000
VERSION     DAJTXQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.052"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(167..577)
                     /locus_tag="OO515_002060"
     CDS             complement(167..577)
                     /locus_tag="OO515_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR6575997.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(635..868)
                     /locus_tag="OO515_002061"
     CDS             complement(635..868)
                     /locus_tag="OO515_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6575998.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1257..1802
                     /locus_tag="OO515_002062"
     CDS             1257..1802
                     /locus_tag="OO515_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6575999.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1777..3702
                     /locus_tag="OO515_002063"
     CDS             1777..3702
                     /locus_tag="OO515_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6576000.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3699..3905
                     /locus_tag="OO515_002064"
     CDS             3699..3905
                     /locus_tag="OO515_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6576001.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3902..5503
                     /locus_tag="OO515_002065"
     CDS             3902..5503
                     /locus_tag="OO515_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6576002.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5484..6803
                     /locus_tag="OO515_002066"
     CDS             5484..6803
                     /locus_tag="OO515_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6576003.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6813..7145
                     /locus_tag="OO515_002067"
     CDS             6813..7145
                     /locus_tag="OO515_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6576004.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7201..8226
                     /locus_tag="OO515_002068"
     CDS             7201..8226
                     /locus_tag="OO515_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6576005.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8268..8666
                     /locus_tag="OO515_002069"
     CDS             8268..8666
                     /locus_tag="OO515_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6576006.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8678..9031
                     /locus_tag="OO515_002070"
     CDS             8678..9031
                     /locus_tag="OO515_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6576007.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9043..9621
                     /locus_tag="OO515_002071"
     CDS             9043..9621
                     /locus_tag="OO515_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6576008.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9618..10013
                     /locus_tag="OO515_002072"
     CDS             9618..10013
                     /locus_tag="OO515_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6576009.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10021..10761
                     /locus_tag="OO515_002073"
     CDS             10021..10761
                     /locus_tag="OO515_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6576010.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10777..11199
                     /locus_tag="OO515_002074"
     CDS             10777..11199
                     /locus_tag="OO515_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6576011.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11181..11615
                     /locus_tag="OO515_002075"
     CDS             11181..11615
                     /locus_tag="OO515_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6576012.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11608..14169
                     /locus_tag="OO515_002076"
     CDS             11608..14169
                     /locus_tag="OO515_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6576013.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14166..14495
                     /locus_tag="OO515_002077"
     CDS             14166..14495
                     /locus_tag="OO515_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6576014.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14495..15193
                     /locus_tag="OO515_002078"
     CDS             14495..15193
                     /locus_tag="OO515_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6576015.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15199..15942
                     /locus_tag="OO515_002079"
     CDS             15199..15942
                     /locus_tag="OO515_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6576016.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15879..16511
                     /locus_tag="OO515_002080"
     CDS             15879..16511
                     /locus_tag="OO515_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6576017.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16572..20069
                     /locus_tag="OO515_002081"
     CDS             16572..20069
                     /locus_tag="OO515_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6576018.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20140..20656
                     /locus_tag="OO515_002082"
                     /pseudo
     CDS             20140..20656
                     /locus_tag="OO515_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20721..22125
                     /locus_tag="OO515_002083"
                     /pseudo
     CDS             20721..22125
                     /locus_tag="OO515_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            22074..22226
                     /locus_tag="OO515_002084"
                     /pseudo
     CDS             22074..22226
                     /locus_tag="OO515_002084"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22203..22382
                     /locus_tag="OO515_002085"
                     /pseudo
     CDS             22203..22382
                     /locus_tag="OO515_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23097..23804
                     /locus_tag="OO515_002086"
                     /pseudo
     CDS             23097..23804
                     /locus_tag="OO515_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23804..24385
                     /locus_tag="OO515_002087"
     CDS             23804..24385
                     /locus_tag="OO515_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6576019.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         6040 a         6198 c         7554 g         5338 t
ORIGIN      
        1 cgtcgccgta ttcccgtatt aacagagacc gtagcccgac ggggaactcc ttctgcgcga
       61 gtgtgcggga ataatcaaaa acgatgcaca ccggggttac cgggtacaca tatttcatca
      121 tgccagcgag tccggttctg gcacggaaga aaccggacgt tatgatttag tgcggaaata
      181 tttgtgtagt gttctgtatg ttctcagtaa agagtaatga attatcaaag gtatagtaat
      241 accttttgtt ttcgtggata tttgtaatcc atctgaaaac ccctgctgta gcaagatttt
      301 tcctgtattc gtaaaatgat aactctcctg atttgaatcc ttttaaggtg gcttctataa
      361 ggcatttatt ttttgaaaat cttacattta caaccttacc ctgtcctttt attaaaaccg
      421 tattatcgtt ttcaagaaca agatgaatat tctctgtggc taaatagtaa atgtaatgtg
      481 agacattgtg acgttttagt tcagaataaa accagtgata gtttaaatta tttcgcactt
      541 tatcgaatat ttgtttaaaa atggcaacct gagccattgt agtaccttcc atgtgatatg
      601 agggggcgta gtctgcacga ttatctaaat tgcttcaatc tggtctaacc tgttttctga
      661 gcaattcagt aatgtcactc ttttctttgt ttgcttcagg cgaaactctt ttttctgagc
      721 acagtctccg gcggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgaa
      781 caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
      841 ttctgcgggt tctgttcttc gttgacatga ggttgccctg tattcagtgt cgctgatttg
      901 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
      961 cataattgat tatttgacgt ggtttgatgg cgtagatgca cgttgtgaca tgtagatgat
     1021 aattattatc attttgcggg tcctttccgg cgatccgaca ggttacgggg cggaaggcgc
     1081 gcgggttttc gctatttatg acaattttcc ggtttaaggc gtttccgttc ttcttcgccg
     1141 taacctaatg tttttattta aaacaccccc tgaaaagaaa ggaaacgaca ggtgctgaaa
     1201 acgggctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     1261 aagtcaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc attcagaact
     1321 ggcaggaaca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag gtgctttatg
     1381 actctgccgc cgttataaga tggtatgccg aaagggatgc tgaaattgag aacgaaaagc
     1441 tgcgccggga agttgaagaa ctgcggcagg ccagcgagac agatctccag ccagggacta
     1501 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggag ctgaaaaatg
     1561 ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
     1621 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
     1681 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
     1741 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
     1801 aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc ggagccacag
     1861 accgccgttg aatgggcgga tgctaattac tatctcccga aagaatccgc ataccaggaa
     1921 gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg cagcgactac
     1981 atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat gctgctgggt
     2041 gtttatgcct actttataga gcataagcag cgcaacacac ttatctggtt gccgacggat
     2101 ggtgatgccg agaactttat gaaaacccac gttgagccga ccatccgcga tattccgtcg
     2161 ctgctggcgc tggctccgtg gtatggcaaa aagcaccggg ataacacgct cactatgaag
     2221 cgtttttcca atggtcgtgg cttctggtgc ctgggcggta aagcggcaaa aaactaccgt
     2281 gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga ggatattgaa
     2341 caggaaggct ctccgacgtt ccttggcgac aaacgtattg aaggctcggt ctggccaaag
     2401 tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg tgcagccagt
     2461 gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga ggagcagtat
     2521 cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga tgacccctcc
     2581 agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga gctggacttt
     2641 actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg cattctctgg
     2701 ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca catctggaca
     2761 gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa aacgaaaggg
     2821 gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg ggaggcgaaa
     2881 attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta ttcagcgccc
     2941 gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagcggga tcgctacgaa
     3001 atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgatcg gcagattatt
     3061 atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat caataaaacc
     3121 tatacccgcc ggaatggtgc agaaatgtcg atatcccgta tctgctggga tattggcggg
     3181 attgacccga ccattgtgta tgaacgctcg aaaaagcatg ggctgttccg ggtgatcccc
     3241 attaaagggg catccgtcta cggtaagccg gtggccagca tgcctcgtaa gcgaaacaaa
     3301 aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat ttataaccgc
     3361 ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt cccgaataac
     3421 ccggatattt ttgatctgac cgaagcgcag cagctgactg ctgaagagca ggtcgaaaaa
     3481 tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa tgaggcgctc
     3541 gactgcttcg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg gcagctggat
     3601 ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa caagaaaaca
     3661 ctggcagatt acgcccgtgc cttatccgga gaggatgaat gacgcgacag gaagaacttg
     3721 ccgctgcccg tgcggcactg catgacctga tgacaggtaa acgggtggca acggtacaga
     3781 aagacggacg gcgagtggag tttacggcca cttccgtgtc tgacctgaaa aaatacattg
     3841 cggagctgga ggtgcagacc ggcatgacac agcgacgcag gggacctgca ggattttatg
     3901 tatgaaaacg cccaccattc ccacccttct gggaccggac ggcatgacat cgctgcgtga
     3961 atatgccggt tatcacggcg gtggcagcgg atttggtggg cagttgcggg cgtggaaccc
     4021 accgggtgaa agtgtggatg cagccctgct gcccaacttt acccgtggca atgcccgcgc
     4081 agacgatctg gtacgcaata acggctatgc tgccaacgcc atccagctgc atcaggatca
     4141 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     4201 ggaggaagac gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     4261 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     4321 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     4381 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     4441 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     4501 ggcgctggga tattacgtca gcgaggacgg ctatcctggc tggatgccgc agaaatggac
     4561 atggataccc cgtgagttac ccggcgggcg cgcctcgttc attcacgttt ttgaacccgt
     4621 ggaggacggg cagacccgcg gtgcaaatgt gttttacagc gtaatggagc agatgaagat
     4681 gctcgacacg ctgcagaaca cgcagctgca gagcgccatt gtgaaggcga tgtatgccgc
     4741 caccattgaa agtgagctgg atacgcagtc agcgatggat tttattctgg gcgcgaacag
     4801 taaggagcag cgggaaaggc tgacgggctg gattggtgaa attgccgcgt attacgccgc
     4861 agcaccggtc cgtctgggag gcgcaaaagt gccgcacctg atgccggggg actcactgaa
     4921 cctgcagacg gctcaggaca cggataacgg ctactccgtg tttgagcagt cactgttgcg
     4981 gtatatcgct gccgggctgg gtgtctcgta tgagcaactt tcccggaatt acgcccagat
     5041 gagctactcc acggcacggg ccagtgcgaa cgagtcgtgg gcgtacttta tggggcggcg
     5101 aaaattcgtc gcatcccgtc aggcgagcca gatgtttctg tgctggctgg aagaggccat
     5161 cgttcgccgc gtggtgacgt taccttcaaa agcgcgcttc agctttcagg aagcccgcag
     5221 tgcctggggg aactgcgact ggataggctc cggtcgtatg gccatcgatg gtctgaaaga
     5281 agttcaggaa gcggtgatgc tgatagaagc cggactgagc acctacgaga aagagtgcgc
     5341 gaaacgcggt gacgactatc aggaaatttt tgcccagcag gtccgtgaaa cgatggagcg
     5401 ccgcgcagct ggtcttaaac cgcccgcctg ggcggctgcg gcatttgaat ccgggctgcg
     5461 acaatcaaca gaggaggaga agagtgacag cagagctgcg taatctcccg catattgcca
     5521 gcatggcttt taatgagccg ctgatgcttg aacccgccta tgcgcgggtt ttcttttgtg
     5581 cgcttgcagg ccagcttggg atcagctgcc tgacggatgc agtatccggc gacagcctga
     5641 ctgccggaga ggcacccgcg gcgctggcgt tatccggtga tgatgacgga ccacgacagg
     5701 cccgcagtta tcaggtaatg aacggcatcg ccgtgctgcc ggtgtccggc acgctggtca
     5761 gccggacgcg ggcgctgcag ccgtactcgg ggatgaccgg ttacaacggc attatcgccc
     5821 gtctgcaaca ggctgccagc gacccgatgg tggacggcat tctgctcgat atggacacac
     5881 cgggcggaat ggtggcggga gcatttgact gcgctgacat catcgcccgt gtgcgtgaca
     5941 taaagccggt atgggcgctg gccaatgaca tgaactgcag cgcaggtcag ctgcttgcca
     6001 gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc ggcgtcatga
     6061 tggctcacag taattacggc gctgcactgg agaaacaggg cgtggaaatc acgctgattt
     6121 acagcggcag ccataaggtg gatggcaacc cctacagcca tctaccggat gatgtccggg
     6181 aaacattgca gtcccggatg gatgcaaccc gccggatgtt tgcacagaag gtgtcggcat
     6241 ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac agcggtcagg
     6301 aggccattga tgccggactg gctgatgaac ttgttaacag taccgatgcg atcaccgtca
     6361 tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg accaaagaga
     6421 ctcaatcaac aactgtttca gccactgctt cgcaggctga cgttactggc gtggtgcaag
     6481 cgacggaggg cgagaacgcc agcgcggcgc agccggacgt gaacgcgcag atcaccgcag
     6541 cggttgcggc agaaaacagt cgcattatgg ggatcctcaa ctgtgaggag gctcacggac
     6601 gcgaagaaca ggcacgcgtg ctggccgaaa cccccggtat gaccgtggaa acggcccgcc
     6661 gtattctggc cgcagcacca cagagtgcac agacgcgcag tgacactgcg ctggatcgtc
     6721 tgatgcaggg ggcaccggca ccgctggctg caggtaaccc ggcatctgat gccgttaacg
     6781 atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc tttacccatt
     6841 accagccgct gggcaatagt gacccggctc ataccgcaac cgcgcccggc ggattgagtg
     6901 cgaaagcgcc tgcaatgacc ccgctgatgc tggacacctc cacccgtaag ctggttgcgt
     6961 gggatggcac caccgacggt gctgccgttg gcattcttgc ggttgctgct gaccagacca
     7021 gcaccacact gacgttctac aagtccggca cgttccgtta tgaggatgtg ctctggccgg
     7081 aggctgccag cgacgagacg aaaaaacgga ccgtgtttgc cggaacggca atcagcatcg
     7141 tttaacttta cccttcatca ctaaaggccg cctgtgtggc tttttttacg ggattttttt
     7201 atgtcgatgt acacaaccgc ccagctgctg gcggcaaatg agcagaaatt taagtttgat
     7261 ccgctgtttc tgcgtctctt tttccgtgag agctatccct tcactacgga gaaagtctat
     7321 ctctcacaaa ttccgggact ggtaaacatg gcgctgtacg tttcgccgat tgtttccggt
     7381 gaggttatcc gttcccgtgg cggctccacc tctgaattta cgccgggata tgtcaaaccc
     7441 aagcatgagg tgaatccgca gatgaccctg cgtcgcctgc cggatgaaga tccgcagaat
     7501 ctggcggacc cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg tgacgaagag
     7561 ctggccattg ctcaggtcga agagatgcag gcagtttctg ccgtgctcaa gggcaaatac
     7621 accatgaccg gtgaagcctt cgatccggtt gaggtggata tgggccgcag tgcggcgaac
     7681 aacatcacac agtccggcgt cacggagtgg agcaagcgtg acaagtccac gtatgacccg
     7741 accgacgata tcgaagccta cgcgctgaac gccagcggtg tggtgaatat catcgtgttt
     7801 gacccgaaag gctgggcgct gttccgttcc ttcaaagccg tcaaggagaa gctggatacc
     7861 cgtcgcggct ctcattccga gctggagaca gcggtaaaag acctgggcaa agcggtgtcc
     7921 tataagggaa tgtatggcga tgtggccatc gtcgtgtatt ccggacagta cgtggaaaac
     7981 ggcgtcaaaa agaacttcct gccggacaac acgatggtgc tggggaacac tcaggctcgc
     8041 ggtctgcgca cctatggctg cattcaggat gcggacgcac agcgcgaagg cattaacgct
     8101 tctgcccgtt acccgaaaaa ctgggtgacc accggcgatc cggcgcgtga gttcaccatg
     8161 attcagtcag caccgctgat gctgctggct gatcctgatg agttcgtgtc cgttcaactg
     8221 gcgtaatcat ggcccttcgg ggccattttc tctctgtgga ggagttcatg acgaaagatg
     8281 aactgattgc ccgtcttcag gagctgggtg agcaattgaa ccgcgatgtc agtttgacgg
     8341 gaacgaaaga agaactggcg cttcgtgtgg cagagctgga agaagagctt gatgacacgg
     8401 atgaaactgc cggtcaggac acccctctca gccgggaaaa tgtgctgacc gggcatgaaa
     8461 atgaggtggt atcagcgcag ccggataccg tgattcagga tacggctgaa ctggtcacgg
     8521 tcgtggcact ggtgacgctg catactgatg cacttcacgc cacgcgggat aaacctgtgg
     8581 catttgtgct gccgggaacg gcgtttcgtg tctctgccgg tgtggcagcc gaaatgacag
     8641 agcgcggcct ggccagaatg caataacggg aggcgctgtg gctgatttcg ataacctgtt
     8701 cgatgctgcc attgcccgcg ccgatgaaac gatacgcggg tacatgggaa cgtcagccac
     8761 catgacatcc ggtgagcagt caggtgcggt gatacgtggt gtttttgatg accctgaaaa
     8821 tatcagctat gccggacagg gcgtgcgcgt tgaaggctcc agcccgtccc tgtttgtccg
     8881 gactgatgag gtgcggcagc tgcggcgtgg agacacgctg accatcggtg aggaaaattt
     8941 ctgggtagat cgggtttcgc cggatgatgg cggaagttgt catctctggc ttggacgggg
     9001 cgtaccgcct gccgttaacc gtcgccgctg aaagggggat gtatggccat aaaaggtctt
     9061 gagcaggccg ttgaaaacct cagccgtatc agcaaaacgg cggtgcccgg tgccgccgca
     9121 atggccatta accgcgttgc ttcatccgcg atatcgcagt ctgcgtcaca ggttgcccgt
     9181 gagacaaagg tacgccggaa actggtaaag gaaagggcca ggctgaaaag ggccacggtc
     9241 aaaaatccgc aggccagaat caaagttaac cggggggatt tgcccgtaat caagctgggt
     9301 aatgcgcggg ttgtcctgtc ccgccgcagg cgtcataaaa aggggcagcg ttcatccctg
     9361 aaaggtggcg gcagcgtgct tgtggtgggt aaccgtcgta ttcccggcgc gtttattcag
     9421 caactgaaaa acggccggtg gcatgttatg cagcgtgtgg ccgggaaaaa ccgttacccc
     9481 attgatgtgg tgaaaatccc gatggcggtg ccgctgacca cggcgtttaa acagaatatt
     9541 gaacggatac ggcgtgaacg tcttccgaaa gagctgggct atgcgctgca gcatcaactg
     9601 agaatggtaa taaagcgatg aaacatactg aactccgtgc agccgtactg gatgcactgg
     9661 agaagcatga caccggggcg acgctttttg atggtcgccc cgctgttttt gatgaggaag
     9721 attttccggc aattgccgtt tatctcaccg gcgctgaata cacgggcgaa gagctggaca
     9781 gcgatacctg gcaggcggag ctgcatattg aagttttcct gcctgctcag gtgccggatt
     9841 cagagctgga ttcgtggatg gagtcccgga tttatcctgt gatgagcgat atcccggcac
     9901 tgtcagattt gatcaccagt atggtggcca gcggctatga ctaccggcgc gacgatgatg
     9961 cgggcctgtg gagttcagcc gatctgactt atgtcattat ctatgaaatg tgaggacgct
    10021 atgcctgtac caaatcctac aatgccggtg aaaggtgccg ggaccaccct gtgggtttat
    10081 aaggggagcg gtgaccctta tgcgaacccg ctttcagacg ttgactggtc gcgtctggca
    10141 aaagttaaag acctgacgcc cggcgaactg accgctgagt cctatgacga cagctatctc
    10201 gatgatgaag atgcagactg gactgcgacc gggcaggggc agaaatctgc cggagatacc
    10261 agcttcacgc tggcgtggat gcccggagag caggggcagc aggcgctgct ggcgtggttt
    10321 aatgaaggtg atacccgtgc ctataaaatc cgcttcccga acggcacggt cgatgtgttc
    10381 cgtggctggg tcagcagtat cggtaaggcg gtgacggcga aggaggtgat tacccgcacg
    10441 gtgaaggtca ccaatgtggg acgtccgtcg atggcagaag atcgcagcac ggtgacggcg
    10501 acgaccggca tgacggtgac acccgccagt gcttccgtag tgaaagggca gagcaccacg
    10561 ctgaccgtgg cattccagcc ggatggcgca accgacaaga gcttccgtgc ggtgtctgcg
    10621 gataaaacaa aagccaccgt gtcggtcagt ggtatgacca tcaccgtgaa aggtgttgct
    10681 gcaggcaagg tcaacattcc ggtcgtatct ggtaatggtg agtttgctgc ggttgcagaa
    10741 atcaacgtca ccgccagtta atccggagag tcagcgatgt tcctgaaaac cgaatcattt
    10801 gaacataacg gtgtgaccgt cacgctttct gaactgtcag ccctgcagcg tattgagcat
    10861 ctcgacctga tgaaacggca ggcagaacag gcggagtcag acagcaaccg gaagtttact
    10921 gtggaagacg ccatcagaac cggcgctttt gtggttgcga tgtccctgtg gcataaccat
    10981 ccgaagaaga cgcagatgcc gtccatgaat gaagccgtta aacagattga gcaggaagtg
    11041 cttaccacct ggcccacaga ggcaatttct catgctgaaa acgtggtgta ccggctgtcc
    11101 ggtatgtatg agtttgtggt gaatgatgct cctgaacagg cagaggacgc cgggcctgca
    11161 gagcctgttt ctgcgggaaa gtgttcgacg gtgagctgag ttttgccctg aaactggcgc
    11221 gtgagatggg gcgacccgac tggcgcgcca tgcttgccgg gatgtcatcc acggagtatg
    11281 ccgactggca ccgcttttac agtacccatt attttcatga tgttctgctg gatatgcact
    11341 tttccgggct gacgtacacc gtgctcagcc tgtttttcag cgatccggag atgcatccgc
    11401 tggatttcag tctgctgaac cggcgcgagg ctgacgaaga gcctgaagat gatgtgctga
    11461 tgcagaaagc ggcagggctt gccggaggtg tccgctttgg cccggacggg aatgaagtta
    11521 tccccgcttc cccggatgtg gcggacatga cggaggatga cgtaatgctg atgacagtat
    11581 cagaagggat cgcaggagga gtccggtatg gctgaaccgg taggcgatct ggtcgttgat
    11641 ttgagtctgg atgcggccag atttgacgag cagatggcca gagtcaggcg tcatttttcc
    11701 ggtacggaaa gtgatgcgaa aaaaacagcg gcagtcgttg aacagtcgct gagccgacag
    11761 gcgctggctg cacagaaagc ggggatttcc gtcgggcagt ataaagccgc catgcgtatg
    11821 ctgcctgcac agttcaccga cgtggccacg cagcttgcag gggggcagaa tccctggctg
    11881 atcctgctgc aacagggtgg tcaggttaag gactccttcg gcgggatgat ccccatgttc
    11941 agggggcttg ccggcgcgat caccctgccg atggtggggg ccacctcgct ggcggtggcg
    12001 accggtgcgc tggcgtatgc ctggtatcag ggcaactcaa ccctgtccga tttcaacaaa
    12061 acgctggtcc tttccggtaa tcaggcggga ctgacggcag atcgtatgct ggtcctgtcc
    12121 agagccgggc aggcggcagg gctgacgttt aaccagacca gcgagtcact cagcgcactg
    12181 gttaaggcgg gggtaagcgg tgaggctcag attgcgtcca tcagccagag tgtggcgcgt
    12241 ttctcctatg catccggcgt ggaggtggac aaggtcgctg aagccttcgg gaagctgacc
    12301 acagacccga cgtcggggct gacggcgatg gcacgccagt tccataacgt gacggcggag
    12361 cagattgcgt atgttgctca gttgcagcgt tccggcgatg aagccggggc attgcaggcg
    12421 gcgaacgagg ccgcaacgaa agggtttgat gaccagaccc gccgcctgaa agagaacatg
    12481 ggcacgctgg agacctgggc agacaggact gcacgtgcat tcaaatccat gtgggatgcg
    12541 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
    12601 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attatttcgt taacgatgaa
    12661 gcacgggcgc gttactggga tgatcgtgaa aaaaaacgcc tggagcggga tgcagcccaa
    12721 aagagggtag atcaacagcg tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
    12781 gcgtcacggc tgaaatatac cgaagaggcg cagaaggctt acgaacggct gcagacgccg
    12841 ctggagaaat ataccgcccg tcaggaagaa ctgaacaagg cactgaaaga cggaaaaatc
    12901 ctgcaggcag attacaacac gctgatggcg gcggcgaaaa aggattatga agcgacgctg
    12961 aaaaagccga aacagtccgg cgtgaaggtg tatgcgggcg atcgtcagga agacagtgct
    13021 catgcggccc tgctgacgct tcaggcagaa ctccggacgc tggagaagca tgccggagcg
    13081 aatgagaaaa tcagccagca gcgccgggat ttgtggaagg cagaaagtca gttcgcggta
    13141 ctggaggagg cggcacaacg tcgccagctg tccgcacagg agaaatccct gctggcgcat
    13201 aaagacgaga cgctggagta caaacgccag ctggctgcac ttggtgacaa ggttacgtat
    13261 caggagcacc tgaatgcgct ggcgcagcag gcggataaat tcgcacagca gcaacgggca
    13321 aaacgggcag ccattgatgc gaaaaaccgg gggctgactg accggcaggc agcgcgggaa
    13381 gccacggaac agcgcctgaa ggaacagtat ggcgataatc ctctggcgct gaataacgtc
    13441 atgtcagagc agaaaaagac ctgggcggct gaagaccagc ttcgcgggag ctggatggca
    13501 ggcctgaagt ccggctggag tgagtgggaa gagagcgcca cggacagtat gtcacaggtt
    13561 aaaagtgcag ccacgcagac ctttgatggt attgcacaga atatggcggc gatgctgacc
    13621 ggcagtgaac agaactggcg cagcttcacc cgctccgtgc tgtccatgat gacagaaatt
    13681 ctgcttaagc aggcaatggt ggggattgtc gggagtatcg gcagcgccat tggcggggct
    13741 gttggtggcg gcgcatccgc gtcaggtggt acagccattc aggccgctgc ggtgaaattc
    13801 cattttgcaa ccgggggatt tacgggaacc ggcggcaaat atgagccagc ggggattgtt
    13861 caccgtggtg aatttgtctt cacgaaggag gcaaccagcc ggattggcgt ggggaatctc
    13921 taccggctga tgcgcggcta tgcggaaggt ggttatgtgg gcggtgccgg aagtccggcg
    13981 cagatgcggc gggctgaagg cattaatttt aatcagaaca atcacgtggt gattcagaac
    14041 gacggtacga atggtctgcc aggtccacag atgatgaagg ccgtgtatga catggcccgc
    14101 aagggtgccc gtgatgaaat tcagacacag atgcgtgatg gtggcctgtt ctccggaggt
    14161 ggacgatgaa aaccttccgc tggaaagtga aacccggtat ggatgtggct tcggcccctt
    14221 ctgtaagaaa ggtgcgcttt ggtgatggct attcccagcg agcgcctgcc gggctgaatg
    14281 ccaacctgaa aacgtacagc gtgacgcttt ctgtcccccg tgaggaggcc atggtactgg
    14341 agtcgtttct ggaagagcac gggggctgga aagcctttct gtggacgccg ccttatgagt
    14401 ggcggcagat aaaggtgacc tgcgcaaaat ggtcgtcgcg ggtcagtatg ctgcgtgttg
    14461 agttcagcgc agagtttgaa caggtggtga actgatgcag gatatccggc aggaaacact
    14521 gaatgaatgc acccgtgcgg agcagtcggc cagcgtggtg ctctgggaaa tcgatctgac
    14581 agaggtcggt ggagaacgtt attttttctg taatgagcag aacgaaaaag gtgagccggt
    14641 cacctggcag gggcgacagt atcagccgta tcccattcag gggagtggtt ttgaactgaa
    14701 tggcaaaggc accagtacgc gccccacgct gatggtttct aacctgtacg gtatggtcac
    14761 cgggatggcg gaagatctac agagtctggt cggcggaacg gtggtccggc gtaaggttta
    14821 cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agtgacgccg atccggagca
    14881 ggaggtgatc agccgctggc gcattgagca gtgcagcgaa ctgagcgcgg tgagtgcctc
    14941 ttttgtactg tccacgccga cggaaacaga tggcgctgtt tttccgggac gtatcatgct
    15001 ggccaacacc tgcacctgga cctatcgcgg cgatgagtgc ggttatcacg gtccggcggt
    15061 cgcggatgaa tatgaccagc caacgtccga catcacgaag gataaatgca gcaaatgcct
    15121 gagtggctgt aagtttcgca ataacgtcgg caactttggc ggcttccttt ccattaacaa
    15181 actttcgcag taaatcccat gacagagaca gaatcagcga ttctggcgca cgcccggcga
    15241 tgtgctccag cggagtcgtg cggcttcgtg gtaagaacgc cggaggggga aagatatttc
    15301 ccctgcgtga atatctccgg tgagccggag gcgtatttcc gtatgtcgcc ggaagactgg
    15361 ctgcaggcag agatgcaggg tgagattgtg gcactggtcc acagccaccc cggtggtctg
    15421 ccctggctga gtgaggccga ccggcggctg caggtgcaga gtgatttgcc gtggtggctg
    15481 gtctgccggg gggcgattca caagttccgc tgtgtgccac atcttaccgg gcggcgcttt
    15541 gagcacgggg tgacggactg ttacacgctg ttccgggatg cttaccatct ggcggggatt
    15601 gagatgccgg attttcatcg tgaggatgac tggtggcgtc acggtcagaa tctctatctg
    15661 gataatctgg aggccacagg gctgtatcag gtgccgttgt cagcggcgca gccgggcgat
    15721 gtgctgctgt gctgttttgg ttcatcggtg ccgaatcatg ccgccattta ctgtggtgac
    15781 ggcgagctgc tgcaccatat tcctgaacaa ctgagcaaac gagagaggta taccgacaaa
    15841 tggcagcgac gcacacactc cctctggcgt caccgggcat ggcacgcatc tgcctttacg
    15901 gggatttgca acgatttggc cgccgcatcg accttcgtgt gaaaacgggt gccgaagcca
    15961 tccgggcgat ggccacacag ctccctgcgt ttcgtcagaa gctgagcgac ggctggtatc
    16021 aggtgcgtat tgccgggcag gatgttagca cgtccggatt aacggcacag ttacatgagg
    16081 ctctgcctga cggcgctgtg attcatattg ttcccagagt cgccggggcc aagtcaggtg
    16141 gtgtattcca gattgtcctg ggagcagccg ccattgccgg atcattcttt accgccggag
    16201 ccacccttgc agcatggggg gctgccattg gtgccggtgg tatgaccggc attctgtttt
    16261 ctctcggtgc cagtatggtg ctcggcggtg tggctcagat gctggcaccg aaagccagaa
    16321 ctccccgcac acagacaacg gataacggta agcagaacac ctatttctcc tcactggata
    16381 acatggttgc ccagggcaat gttatgcctg ttctgtatgg tgaaatgcgc gtggggtcac
    16441 gtgtggtatc tcaggagatc agcacggcag acgaagggga cggtggtcag gttgtggtga
    16501 ttggtcgctg atgcaaaatg ttttatgtga aatcgcctcc gggcggtttt atcgtttatg
    16561 gagcatgacg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
    16621 acctgaagtc cacgcagctg ctgagtgtga tcgatgccat cagcgaaggg ccggttgaag
    16681 gtccggtgga tggattaaaa agcgtgctgc tgaacagtac gccggtgctg gacagtgagg
    16741 ggaataccaa catctccggc gtcacggtgg tgttccgggc aggtgagcag gagcagtcac
    16801 cgccggaggg atttgaatcc tccggctccg agacggtgct gggtacggaa gtgaaatatg
    16861 acacgccgat cacccggacc atcacgtcgg caaacattga ccgactgcgt tttaccttcg
    16921 gtgtacaggc actggtggaa accacctcaa agggagacag gaatccgtct gaagttcgcc
    16981 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
    17041 gcaaaaccac ctcgcagtat ctggcctcgg tggtggtgga taacctgccg ccgcgcccgt
    17101 ttaatatccg gatgcgcaga atgacgccgg acagcaccac agaccagctg cagaacaaaa
    17161 cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcac
    17221 tggtcggcgt acaggtggat tcggagcagt tcggcagcca gcaggtgagc cgtaattatc
    17281 atcttcgcgg gcgcattctg caggtgccgt cgaactataa tccgcagacg cggcaataca
    17341 gcggtatctg ggacggaacg ttaaaaccgg catacagcaa caacatggcc tggtgtctgt
    17401 gggatatgct gacccatccg cgctatggca tggggaaacg tcttggtgcg gcagatgtgg
    17461 ataaatgggc gctgtatgtc atcggccagt actgtgacca gtcggtgccg gacggctttg
    17521 gcagcacgga gccgcgcatc acctgtaatg cctggctgac cacacagcgc aaggcgtggg
    17581 atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
    17641 tgacgttcgt gcaggaccga cagtcggata aggtgtgggc ctataaccgc agtaatgtgg
    17701 tgatgccgga tgatggcgcg ccgttccgct acagtttcag cgccctgaaa gaccgccata
    17761 atgccgttga ggtgaactgg attgacccgg ataacggctg ggagacggcg acagagcttg
    17821 tggaggatac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    17881 gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa acggaactgc
    17941 tggagacgca gaccgtggat ttcagcgtgg gtgctgaagg gcttcgtcat gtaccgggcg
    18001 atgtcattga aatctgcgat gatgactatg ccggtatccg caccggcggg cgcgtgctgg
    18061 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccatcctccg
    18121 gcaccacgct gataagcctg gttgacgggc aggggaatcc ggtcagcgtg gaggtccagt
    18181 ccgtcaccga cggcgtgaag gtaaaagtga gccgtgttcc tgacggtgtt gctgaataca
    18241 gcgtgtgggg gctgaagctg ccgacgctgc accagcgatt gttccgctgc gtgagtatcc
    18301 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagaag
    18361 ccatcgtgga taacggggcg cactttgacg gcgaccagag cggcacggtg aatggtgtca
    18421 cgccgccagc ggtgcagcac ctgactgccg aagtcaccgc agacagcggg gaatatcagg
    18481 tgctggcgcg ctgggacacg ccgaaggtgg tgaagggcgt gagcttcctg ctccgtctga
    18541 ccgtaacagc ggatgacggc agtgagcggc tggtcagcac ggcccggacg acggaaacca
    18601 cttaccgctt cacacaactg gcgctgggga actacaggct gacagtccgg gcagtaaatg
    18661 cgtgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgccgca ccggcagcgc
    18721 tgtcgcggat tgagctgacg ccgggctatt ttcagataac cgccacgccg catcttgcgg
    18781 tttatgatcc gacggtacag tttgagttct ggttctcgga aaagcggatt gcggatatca
    18841 ggcaggttga aaccacagca cgctatcttg gcacggcgct gtactggata gccgccagta
    18901 tcaatatcaa accgggccat gattattact tttatatccg cagtgtgaac accgttggca
    18961 aatcggcatt cgtggaggct gttggtcagc cgagtgatga tgcatccggt tatctggatt
    19021 ttttcaaagg cgagataggg aaaacccatc tggctcagga gctgtggacg cagattgata
    19081 acggtcagct tgcgcctgac ctggctgaaa tcaggacgtc cattacggat gtcagcaatg
    19141 aaatcacgca gaccgtcaat aagaaactgg aagaccagag tgcggcaatt cagcagatac
    19201 agaaggttca ggttgataca aataataacc tgaacagcat gtgggctgtg aagctgcagc
    19261 agatgcagga cggacgcctt tatatcgcgg gtattggtgc cggtattgag aacacccctg
    19321 acggcatgca gagtcaggtg ctgctggcgg cggacaggat tgcgatggtt aatcctgcga
    19381 atggcaacac aaaaccgatg tttgttggtc agggcgatca gatattcatg aacgacgtgt
    19441 tcctgaaacg cctgacggcc cccaccatta ccagcggtgg aaatccatcg gcattttccc
    19501 tgactccgga cggaaagctg accgctaaaa atgcggatat cagtggcagt gtgaatgcga
    19561 acgccgggac gctcaacaat gtcacggtaa atgaaaactg tacgattaag ggcatgctgg
    19621 aggcgactca ggtcagaggt gacttcgtta aagctgtatc caaatcattt ccgaaacagg
    19681 ctggtacgtg gggtaacacg gaaacaccaa acgggacggt tacagtcacc ataagcgatg
    19741 atcataactt tgaccgccag attattattc cgcccattat ttttaacggt atagcgtatg
    19801 acgatccggg gagcggaaat aacccaggag gcacgcgata cacgggttat ggttttgaag
    19861 ttcgcaaaaa cggcgtatta atcgcatcca gagaaactaa aggtgccatt cccggtagtt
    19921 acagtgcagt tattgatatg cctagtggtg gtggtagcgt cactctggag tttaagattt
    19981 tccagaaagg caatcagggg gcaggcaata tcaccgactg tacggtgatt gtgaccaaaa
    20041 aagcggcttc cggcatcagt attcgttgaa atatttataa ccccaataaa tggcgtcagg
    20101 aatgacgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaagt ttgtgcagcc
    20161 attttgtccg cagccatttg cctggccgta tccggtgcgc ctgcatgggc gtctgaacat
    20221 cagtccacgc tgagcgcggg gtatcttcat gcccggacca acgttcccgg cagtgatgat
    20281 ctgaacggga ttaacgtgaa ataccgttat gagtttacgg atacgctggg gctggtgacg
    20341 tcattcagct atgcaggaga caagaatcgc cagctgaccc gttacagcga tacccgctgg
    20401 catgaagatt ccgtgcgtaa ccgctggttc agcgtgatgg cggggccgtc tgtgcgcgtg
    20461 aatgaatggt tcagcgcgta tgcgatggcg ggtgtggctt acagccgtgt gtcgactttt
    20521 tccggggatt atatccgcgt aactgacaat aaggggaaaa cgcacgatgt gctgaccgga
    20581 agtgatgacg gtcgccacag caacacgtct ctggcgcact gacggtttca tcgtgggtgt
    20641 cggttataaa ttctgattag ccaggtaaca cagtgttatg acagcccgcc ggttcaggcg
    20701 ggcttttttg tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag
    20761 gaaaaccggt acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg
    20821 tggtgaacac gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg
    20881 agtacggtca gtacagcgtt attctgttgg tggaaggatt cccgccgtca catgccggga
    20941 ccatcaccgt gtatgaagat tcccgacccg gtacgctgaa tgattttctc ggtgccatga
    21001 cggaggatga tgcccgtccg gaggcactgc gccgttttga gctgatggtg gaagaggtgg
    21061 cgcgtaacgc gtccgcggtg gcacagaaca cggcagccgc gaagaaatca gccagcgatg
    21121 ccagcacatc agcccgtgag gcggcaaccc atgcgactga tgctgcagat tcagcacgtg
    21181 cagccagcac gtcagccgga caggccgctt cgtcggctca gtcagcgtct tccagcgcag
    21241 gaacggcatc aacaaaggcc actgaagcct caaaaagtgc tgccgctgca gagtcctcaa
    21301 aaagcgcggc ggctaccagt gccggtgcgg cgaaaacgtt agaaacgaat gcggcagcgt
    21361 cacaacaatc agcagccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcca
    21421 cctcagcccg ggatgcgtcg gcttcaaaag aggcggcaaa atcatcagaa acgaacgcat
    21481 catcaagcgc cagtagcgca gcttcctcgg cgacggcggc tgcaaattct gcgaaggcgg
    21541 ccaaaacgtc cgagacgaac gccaggtctt ctgaaacggc agcgggacag agtgcctcag
    21601 ctgcggcagg ctcaaaaaca gcggctgcgt cgtctgccag cgccgcgtca acaagtgccg
    21661 ggcaggcctc agccagtgcc accgccgccg gaaaatcggc agaaagcgcc gcatcatccg
    21721 cttcaacagc cacaacgaag gctggcgaag ccactgagca ggccacggca gcagcgaggt
    21781 cagcttccgc agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc cgtgcagaat
    21841 cctcaaaaac ggctgccgca tcgtccgcca gttcggcggc gtcatcggca tcatcggcgt
    21901 ctgcttcaaa agatgaggcg accagacagg cgtcagcagc gaagggcagc gccacgacgg
    21961 catccacgaa ggcgacagag gctgctggta gtgcgacggc agcagctcag agcaaaagta
    22021 cggcggaatc tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat
    22081 ccgccgtggc gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagtagtg
    22141 cgactaacag cacttccgag tcactggcgg caacaccaaa agccgttaag gccgcgtatg
    22201 acctggctaa cgggaaatac accgcacagg atgcaacgac agcacagaaa gggataatcc
    22261 agctaagcag cgcgaccaac agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa
    22321 aagcagccaa tgacaatgct gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc
    22381 ctggcaaaga cacctttacg aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa
    22441 gcacaacaac aggaaactgg acgactgcac agtttatcga gtggctggat tctcagggag
    22501 catttaacca tccatactgg atgtgcaagg gttcctggtc ttatggcaat aataaaatca
    22561 ttactgatac tggctgcggt aatattcatc tcgccggagc tgtcattgaa gtaatgggga
    22621 taaagtcagc gatgacgatc cgcattacca ctccgaccac atccagcggt ggcggaacaa
    22681 ccaacgccca gtttacctat attaatcacg gaacagatta ttcacctggc tggcgaaggg
    22741 actataactc cagaaataag ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa
    22801 cagcagtatc atcagttaat ctggcttcaa aaggtcgggt agccgcgctg acagacaata
    22861 cgcagggggc agcaggtctt gagttatacg aggtgtataa caacggatat ccaacagcgt
    22921 atggaaatat cattcacctg aaagggatga cagccgttgg cgaaggtgag ttactcatcg
    22981 gctggagtgg tacaagcggt gctcatgctc cggcatttat tcgttcacga cgggatacga
    23041 ccgacgcaaa ctggtcgccg tgggcgcagc tttacacctc ggctcatcct cctgcagagt
    23101 tttatccagt cggtgcacca atcccgtggc catcagatac cgttccgtct ggttatgccc
    23161 tgatgcaggg gcagactttt gacaaatctg cttacccgaa acttgcagcc gcttatccgt
    23221 caggcgtgat ccctgatatg cgtggctgga cgattaaggg caagcccgcc agtggtcgtg
    23281 ccgtattgtc tcaggaacag gatggcatta aatcgcacac ccacagcgcc agcgcatcca
    23341 gtacggattt ggggacgaaa accacatcgt cgtttgatta cggcactaaa tccacgaata
    23401 acaccggggc gcatacgcac agtctgagcg gctctacaag ttcagctggg gcccaccaac
    23461 atagtcagac tgggcccaga acgaatagcg ggagtcaacc tactggaatg tttccggcag
    23521 ggagtactca agttagcggg actaatcagg ttggtatcag tggctcatta accagtggca
    23581 cctcccaatg ggtaggtaaa tcatcttcag aaggaaacca tacacactca ctgtccggca
    23641 cagcagcatc tgcgggtgca cacgcgcata ctgtcggtat tggtgctcat acgcactctg
    23701 ttgcgattgg ttcacatgga cacaccatca ccgttaacgc tgctggtaac gcggaaaaca
    23761 ccgtcaaaaa catcgcattt aactatattg tgaggctcgc ataatggcat tcagaatgag
    23821 tgaacaagca cggaccataa aaatttataa cctgctggcc ggaactaatg aatttattgg
    23881 tgaaggtgac gcatatattc cgcctcatac aggtctgcct gcaaacagta ccgatattgc
    23941 accgccagat attccggctg gtttcgtggc tgttttcaac agtgatgagt catcgtggca
    24001 tctcgttgaa gatcatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt
    24061 tatttctgaa ctcggctcat taccggaaaa tgtcacctgg ttatccccgg aaggggagtt
    24121 tcagaagtgg aacggcacag cctgggtgaa ggatacggaa gcagaaaaac tgttccggat
    24181 ccgggaggcg gaagaaacaa aaaacagcct gatgcaggta gccagtgagc atatagcgcc
    24241 gcttcaggat gctgtggatc tggagatcgc aacggagaaa gaaaccttgt tgctggaagc
    24301 ttggaaaaag tatcgggtgt tgctgaaccg tgttgataca tcaactgcac cggatattga
    24361 atggccagta gcacctatag ggtaaattcg taatgattac ctaaatacgt tattcttttg
    24421 ttaaaaaagt gattttgcta taggtaggga gtatgttctt ggattaagaa taaaaaatcg
    24481 ccatgaaaga gcttcatggc gagtacataa acgtacaaaa tgtgatgaaa gtagagtggc
    24541 gtaagccact atattaagat atgacaatag cgaagaaaag taaataacat tttatggacg
    24601 tttaattgtt aattataaaa actagagtgc ccgatattgt ttatcttgct ttaaaataat
    24661 gtactcaaac caaatttttg agctaaaagc tcaaataata taaatgcaat aataatgttt
    24721 aataagacaa ttagcttgct ttgtattttc atttcggaat actgttaagt aactttgatg
    24781 accctaacaa tagatcggaa ttttcttaga gtcaatggtc actgtttttg cttgcaaagc
    24841 aaatgttttg gtggttataa gtcgtcttaa tatagtcacg catgcaactt gttccatgcg
    24901 gagattaaag attggaatat aacgcgaaga tggaattaaa ttacatttgc aataattaat
    24961 ttattattac tcaataatta ccgatattat tattgtaaat taatcattag aatgcaaaaa
    25021 gaaatgtaac ggcaaaataa gaggacgata gctgagaaaa aagcgtgctt tcccaatctg
    25081 gggcaaatca cgtctgaatg acacctgaaa acaggtggta gcctcaagaa
//
LOCUS       DAJTXQ010000053        25103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000053 DAJTXQ010000000
VERSION     DAJTXQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.053"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            194..382
                     /locus_tag="OO515_002088"
     CDS             194..382
                     /locus_tag="OO515_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6576020.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="OO515_002089"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="OO515_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6576021.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="OO515_002090"
     CDS             1143..1307
                     /locus_tag="OO515_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6576022.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="OO515_002091"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="OO515_002091"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6576023.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6110
                     /gene="bcsB"
                     /locus_tag="OO515_002092"
                     /pseudo
     CDS             3772..6110
                     /gene="bcsB"
                     /locus_tag="OO515_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            6117..7223
                     /gene="bcsZ"
                     /locus_tag="OO515_002093"
     CDS             6117..7223
                     /gene="bcsZ"
                     /locus_tag="OO515_002093"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6576024.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7205..10697
                     /gene="bcsC"
                     /locus_tag="OO515_002094"
                     /pseudo
     CDS             7205..10697
                     /gene="bcsC"
                     /locus_tag="OO515_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10779..12767
                     /gene="hmsP"
                     /locus_tag="OO515_002095"
     CDS             10779..12767
                     /gene="hmsP"
                     /locus_tag="OO515_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6576025.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12950..14236
                     /gene="dctA"
                     /locus_tag="OO515_002096"
     CDS             12950..14236
                     /gene="dctA"
                     /locus_tag="OO515_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6576026.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14456..15952
                     /locus_tag="OO515_002097"
     CDS             14456..15952
                     /locus_tag="OO515_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6576027.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="OO515_002098"
     CDS             complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="OO515_002098"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6576028.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17209..17976
                     /gene="pdeH"
                     /locus_tag="OO515_002099"
     CDS             17209..17976
                     /gene="pdeH"
                     /locus_tag="OO515_002099"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6576029.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18046..20106
                     /locus_tag="OO515_002100"
     CDS             18046..20106
                     /locus_tag="OO515_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6576030.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20288..21610)
                     /locus_tag="OO515_002101"
     CDS             complement(20288..21610)
                     /locus_tag="OO515_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6576031.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="OO515_002102"
     CDS             complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="OO515_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6576032.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23083..23982)
                     /locus_tag="OO515_002103"
     CDS             complement(23083..23982)
                     /locus_tag="OO515_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6576033.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24502..>25103
                     /locus_tag="OO515_002104"
     CDS             24502..>25103
                     /locus_tag="OO515_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6576034.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5853 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cggctgccag
     4201 tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat gcccattaac ccactgttta
     4261 ttaccgactt caaccgtgtg cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa
     4321 acccggccag caccacgctt tggctggatg ttgggcggag cagtggactg gatctgacct
     4381 atcagaccct gaatgtgaag agtgacctgt cacacttccc ggtgccattc tttgacccgc
     4441 gcgataaccg tactaacacc ttgccgatgg tctttgcggg tgcgccggat gttgggctgc
     4501 aacaagcatc tgccattgtc gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga
     4561 acttcccggt gctctataac caattgccgg atcgcaatgc cattgtcttt gcaaccaacg
     4621 acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa agccccggtg attgagatga
     4681 ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag
     4741 acctgttgca ggcagcgaaa ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg
     4801 tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg
     4861 tacgtaccga tcgtccggtc acatttggcg aactgaaaac ctatgaagaa cagttacaat
     4921 ccagcggtct tgagccagca gcgattaacg tttcgctaaa cctgccgccg gatctctacc
     4981 tgatgcgcag taccggcatt gatatggata ttaattaccg ctacaccatg ccgccggtga
     5041 aagacagttc gcggatggat atcagcctga ataaccagtt cctgcaatcc ttcaacctga
     5101 gcagtaaaca ggaggcgaac cgcctgctgc tgcggattcc ggtattacaa ggtttgctgg
     5161 atggcaaaac ggatgtctct attccggcgc tgaaactggg cgcgaccaac cagctgcgct
     5221 tcgactttga gtatatgaac ccgatgccgg gcggttcggt ggataactgt attaccttcc
     5281 agccggtgca gaatcatgtg gtgattggtg acgattccac catcgacttc tcgaagtatt
     5341 accacttcat cccgatgccg gatctacgcg cctttgctaa cgcgggcttc ccgttcagcc
     5401 ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa aacgcctaac gaagcacaga
     5461 tggaaacgtt gctgaatact gttggtttta tcggcgcaca gacgggcttc ccggcgatta
     5521 atctgacggt gaccgatgat ggcagcacca ttcagggcaa agatgccgac atcatgatca
     5581 tcggtggtat cccggacaaa ttaaaagacg ataagcagat cgacctgttg gtgcaggcga
     5641 ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg
     5701 agagcgatcg tgcggcagaa acccagtcaa cgctgacctc ttccggtgcg atggcggcgg
     5761 tgattggctt ccagtcgccg tataacgacc agcgcagcgt gattgcgctg ctggcagata
     5821 gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga tagcggcaaa cgcgccacca
     5881 tgttcggttc ggtcgcggtg atccgcgagt ccggtatcaa cagcctacgt gttggcgacg
     5941 tttattacgt aggtcatctg ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc
     6001 cgattctgct ggcggtgctg gcggcaatca gtgtgatatt gctggcatgg gtactgtggc
     6061 gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga
     6121 atgtgttgcg tagtggaatc gtgacgatgc tgctgttggc tgcctttagt gttcaggcag
     6181 cctgtacctg gcctgcctgg gagcagttta aaaaggatta catcagtcag gaagggcgcg
     6241 tcatcgcccc cagcgacgcg cgcaaaatca ccacctccga agggcaaagt tacggtatgt
     6301 tctttgccct ggcggctaac gaccgtgtag ctttcgataa tattctcgac tggacgcaga
     6361 acaatctcgc tcagggctct ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag
     6421 agaacagtaa gtgggaagtg ctggacagca attcggcctc cgatggtgat gtctggatgg
     6481 cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca gcgttatacc gacatcggca
     6541 gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca
     6601 tgttgttacc gggcaaagtg ggttttgctg aggataacag ctggcgtttt aaccccagct
     6661 acctgccgcc gacgctggcg cagtatttca cccgctttgg cgcgccgtgg accacgctgc
     6721 gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa aggtttttcg ccagactggg
     6781 tgcgctatga gaaagacaaa ggctggcagc taaaagccga aaaaacattg atcagcagct
     6841 acgacgctat ccgcgtttac atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag
     6901 cgcggatgct caaccggttt aaaccgatgg cgacattcac tgagaaaaac ggttatccgc
     6961 cggaaaaagt ggatgtggct acggggaaag cgcagggtaa aggaccgatc ggtttttctg
     7021 ccgccatgct gcccttttta caaaaccgcg atgcgcaggc cgttcagcgc cagcgcgtgg
     7081 ccgataactt tcccggcagc gatgcctatt acaactatgt gctgaccctg tttggacaag
     7141 gctgggatca acaccgtttc cgcttctcga caaaaggtga gttattacct gactggggcc
     7201 aggaatgcgc aaattcacac taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc
     7261 gatggtcgag gcagcaccaa ccgctcagca acagttgctg gagcaagttc ggttaggcga
     7321 agcgacccat cgtgaagatc tggtgcaaca gtcgttatat cggctggaac ttattgatcc
     7381 gaataacccg gacgtcgttg ccgcccgttt ccgttctttg ttacgtcagg gcgatattga
     7441 tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg ccgagttcaa atgcgtataa
     7501 atcgtcgcgg actacgatgc tactttccac gccggatggt cgtcaggcac tgcaacaggc
     7561 acgattgcag gcgacgaccg gtcatgcaga agaagctgtg gcgagttaca acaaactatt
     7621 caacggtgcg ccgccggaag gtgacattgc tgtcgagtac tggagtacgg tggcgaaaat
     7681 tccggctcgc cgtggcgaag cgattaatca gttaaaacgc atcaatgcgg atgcaccggg
     7741 caatacgggc ctgcaaaaca atctggcgct attgctgttt agtagcgatc gccgtgacga
     7801 aggttttgcc gtcctggaac agatggcaaa atcgaacgcc gggcgcgaag gggcctctaa
     7861 aatctggtac gggcagatta aagacatgcc cgtcagtgat gccagtgtgt cggcgctgaa
     7921 aaaatatctc tcgatcttta gtgatggcga tagcgtggcg gctgcgcaat cgcaactggc
     7981 agaacagcaa aaacagctgg ccgatcctgc tttccgcgct cgtgcgcaag gtttagcggc
     8041 ggtggactct ggtatggcgg gtaaagccat tcccgaacta caacaggcgg tgcgggcgaa
     8101 cccgaaagac agtgaagctc tgggggcgct gggccaggcg tattctcaga aaggcgatcg
     8161 cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg gacccgcaca gcagcaacaa
     8221 cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg ctggcgatcc agcagggcga
     8281 tgctgcgctg aaagccaata atcctgaccg ggcagaacgc ctgttccagc aggcgcgtaa
     8341 tgtcgataac accgacagtt atgcagtgct ggggctgggc gatgtggcga tggcgcgaaa
     8401 agattatccc gccgccgaac gttattatca gcagaccttg cgtatggaca gcggcaacac
     8461 taacgccgtg cgcgggctgg caaatattta ccgccagcaa tcgccagaaa aagctgaagc
     8521 gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt gatgatatcg aacgcagcct
     8581 gcaaaacgac cgtctggcac agcaggcaga ggcactggaa aaccagggca aatgggcgca
     8641 ggcggcagca cttcagcggc aacgactggc gctggacccc ggcagcgtat ggattactta
     8701 ccgactttcg caggatctct ggcaggccgg acaacgcagc caggccgata cgttaatgcg
     8761 caatctggcg cagcagaagt cgaacgaccc agagcaggtt tacgcttacg ggctgtacct
     8821 acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg gcgcatatca
     8881 acagcctgcc gcgtgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa
     8941 gcgatcaggt gctggaaacc gctaaccgcc tgcgagaaag cggcaaagag gcagaagcgg
     9001 aagcgatgct gcgccagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
     9061 cgcaacaacg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
     9121 agccagctaa cgccgacgcc attcttggtc tgacggaagt ggatattgct gccggtgaca
     9181 aagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca
     9241 cacagcggcg cgtggcgctg gcacaggcgc agcttggcga taccgcagca gcgcagcgga
     9301 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
     9361 tgctgcgtga tggtgcgaag tttgaagcgc aggcgggcga tccaacgcag gcgctggaaa
     9421 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
     9481 cctttacccg actgacccgt aacgacgaga aagatgactg gctgaaacgt ggcgtgcgca
     9541 gcgatgcggc ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact
     9601 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
     9661 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
     9721 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
     9781 tacaggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
     9841 ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg
     9901 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
     9961 cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga
    10021 gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct
    10081 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    10141 aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta
    10201 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    10261 atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt
    10321 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    10381 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    10441 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    10501 gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg
    10561 gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg
    10621 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    10681 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    10741 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    10801 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    10861 ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa
    10921 gcattgctcg ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg
    10981 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    11041 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc ccggtgccga
    11101 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt tactcgctcg
    11161 aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat tccttccgta
    11221 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgc
    11281 ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca
    11341 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    11401 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    11461 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    11521 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    11581 aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga
    11641 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    11701 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    11761 tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcaccatc atgagcgagc
    11821 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct
    11881 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    11941 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    12001 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgctattt
    12061 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    12121 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    12181 gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    12241 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gcgctgcaac
    12301 tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    12361 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    12421 tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca
    12481 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga
    12541 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    12601 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    12661 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcacc
    12721 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    12781 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    12841 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    12901 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    12961 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    13021 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    13081 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    13141 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    13201 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    13261 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    13321 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    13381 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    13441 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    13501 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat
    13561 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    13621 ctgtttctat attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    13681 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    13741 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    13801 ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    13861 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    13921 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    13981 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    14041 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    14101 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    14161 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    14221 cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt
    14281 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    14341 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    14401 gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg ttcacatgca
    14461 gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg ctggctatgt
    14521 gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt ccaacggttt
    14581 acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct
    14641 ggttaatatc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc acgccatccc
    14701 tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt cattgtggca
    14761 gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg acaccacgct
    14821 gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc tctcttatct
    14881 ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg cgctgcaaag
    14941 tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc gctatcgtct
    15001 gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc
    15061 ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga cgctactggt
    15121 ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact
    15181 gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc gtgcggaagc
    15241 ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt gggatacgcc
    15301 gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg acctggcccg
    15361 cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca aagacatcgg
    15421 tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc
    15481 accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg
    15541 cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga aactggagct
    15601 gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg gtcagcggat
    15661 gcgttcgctg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga aactgcggca
    15721 ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc agcagttgtc
    15781 gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta acatgaaagc
    15841 gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg ccacctctgt
    15901 cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt aatgcctgat
    15961 ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag gggtaacact
    16021 ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg ggataatcgc
    16081 gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg cgttttccgc
    16141 actgccgccc gtcaaacata ccgccagata accggcgctg aaagagtcgc cagctgcggt
    16201 ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta acccttcgcc
    16261 agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt tcacgcccgc
    16321 gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg cgtcttcatc
    16381 gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca cctgctgtgt
    16441 ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca cttttccgcc
    16501 gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg ggcttaagat
    16561 cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt cgcaaatcgc
    16621 cgcagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc agtagtagaa
    16681 cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac ggttttccat
    16741 ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca tttgctggct
    16801 aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag gatcgacctg
    16861 acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct taacgtccgc
    16921 gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt tggacatagt
    16981 cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt gctggtcaac
    17041 catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga aagattccgt
    17101 tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttgc gcgccgataa
    17161 tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat gataaggcag
    17221 gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga acggcgtttt
    17281 tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg cggacggtta
    17341 atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca acgcctgccg
    17401 ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt gaaagagcag
    17461 attgatttgc tggcgcaaaa agccgacttc tttgtagagc acgacctgct ggcatcggtc
    17521 aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct gcgccaaatt
    17581 gagcgtcttc cctggctgcg tttcgaactg gtggaacaca tccgcctgcc gaaagattca
    17641 acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg caccgggatg
    17701 gcaaatttct ctgcgctaag tgaagtgcgt tatgactaca tcaaaatcgc gcgagagctg
    17761 ttcgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct tttacatcta
    17821 atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga agagtggcgt
    17881 gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg cccggcaccg
    17941 atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc cgctgggctg
    18001 gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag caaggcaggc
    18061 aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc gatcattttg
    18121 attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt ctctgcggag
    18181 ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg gcaaaaacaa
    18241 gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga catcattctt
    18301 ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt agaggcaacg
    18361 ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggttcaa gctcgaaaag
    18421 cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt taatcttgcc
    18481 aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg gctggataat
    18541 attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc ggatctggaa
    18601 atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg atcgaaaggt
    18661 aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca gggacgttat
    18721 aacggtgaac cgctcacggg tacgggaaaa ataggcggta tgctggcgct gcgtggcgaa
    18781 gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt tgcttttgat
    18841 ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa attttctggc
    18901 gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac cccgccgttt
    18961 gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt ctttgattat
    19021 cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt ctacaccacc
    19081 ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac
    19141 ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa acggtctgaa
    19201 cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc
    19261 gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg gcgcattgag
    19321 catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa aaatgctgac
    19381 ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcgac gaatattcat
    19441 ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg tcgactgaaa
    19501 ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga aatgaacggt
    19561 gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa cagtaacggc
    19621 aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga gattgttggg
    19681 ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt gcgggtgaac
    19741 tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc gcccgcagat ttttgctttc
    19801 gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc agaacagctg
    19861 gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg ttcgccgctg
    19921 tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc gctgattgcc
    19981 cgtggtgctg ttgctgcggc actggcaacg ctggtaacac cgacggcggc gttactggca
    20041 ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc gcagatgaag
    20101 aagtgatgat aattgccgga tgtctgacat ccggcaatta aacagattta gatttttggc
    20161 aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaatcctt
    20221 gtgaattcaa tatattgtat ggaaattcag tcctgataag cgtagcgcat caggcttttc
    20281 acacttatta caacgactga tgtcgcgtct catgggtcag cagcagggcg attaacgtca
    20341 agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta gtctgcaacc
    20401 aggctgcgat atacggtgca acggaagccc cgagaatcga cgctacgttg taagagaatg
    20461 atgctccggt gtaacgcact tctgttggga acagctctgg taacagcgcg cccatcggcc
    20521 cgaaggtcag ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt
    20581 tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc agcgtggtga
    20641 taattaccat gcttttacgg cgaccaaagg catcagccag taatccggcg actggcacca
    20701 tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta cgcggcaggc
    20761 caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc atgatgtaaa
    20821 acagcgtata ggttgccagc atgatgaacg tacccagtac ggttacgcga acatgtttgg
    20881 tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca
    20941 ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagt accgccgaga
    21001 agataaatgg cacacgccag ccccagctca taaactgctc gtcggtcagt agccaggaaa
    21061 gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc tgtgggaagg
    21121 agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc
    21181 cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc agcagcgggg
    21241 cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtt gaaatcccca
    21301 tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa
    21361 aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta
    21421 gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg
    21481 cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga gaggcgacaa
    21541 ggactttatt acgcgagttg atcggcgtgt attcttgctc gtggtcgagt gttgtggctg
    21601 ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa
    21661 agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca aaaaggggat
    21721 aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta taaatagtgg
    21781 ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga
    21841 gttggaatga aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc
    21901 aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg
    21961 ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt
    22021 ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca
    22081 cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac
    22141 ggaaccaaat gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac
    22201 gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt
    22261 gcgcggacgg tggcgcggta accgccagaa gatccagaag aaaagcagat agttggcgaa
    22321 gatggaaatc gccaggccaa tcaaccgcca cgtcggtttc agccactcaa tgctgttcag
    22381 gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt
    22441 cacaatcagc gcaatcaaca aaccaatcag cgaaataaaa tcacgtagat attttaccca
    22501 gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg cgcgaatcgc
    22561 ttcacgcagg ttacccatcc agttgatgcc ggaataaagc gccacagcca ggccgacaag
    22621 ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag
    22681 cgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg
    22741 ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa
    22801 aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc
    22861 tgtcgcgcga atcaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt
    22921 gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat
    22981 cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc
    23041 ttttgtcctt ctgttgtggt cattcttaaa aagtatagtc ggtcagtcca ggtactcttt
    23101 cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg
    23161 cggataaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca cttcaataag
    23221 ctgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc
    23281 ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt
    23341 aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg
    23401 cggtgtataa cgcaccattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa
    23461 gcgcgtcaga tagtgcggac tggcacagtt gaccatggtc agtttgccga ggggacgggc
    23521 gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag
    23581 aatatctacc ggacggttat ggctactcag ttccagctca attcccggat gctgatagag
    23641 aaattccgac agacgcggca gtaacaggtt tttcgcgatt cctgacggga tgtcgatgcg
    23701 taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt
    23761 actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg
    23821 tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg
    23881 cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc
    23941 cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg
    24001 ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg
    24061 ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa
    24121 tagctttatg agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta
    24181 aagtgaatag aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat
    24241 tttggcttgt agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag
    24301 gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt
    24361 aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa
    24421 tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg
    24481 tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt
    24541 catggaatga aaatcagttt acagcagcgt attccaggag tgagtattca gggggccagt
    24601 caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat
    24661 ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct
    24721 gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatt
    24781 cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg
    24841 gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa
    24901 aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta
    24961 gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta
    25021 aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca
    25081 atgatgttaa atatctcctc ctg
//
LOCUS       DAJTXQ010000054        24838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000054 DAJTXQ010000000
VERSION     DAJTXQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.054"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(29..829)
                     /locus_tag="OO515_002105"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="OO515_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="OO515_002106"
     CDS             1396..1761
                     /locus_tag="OO515_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6576035.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="OO515_002107"
     CDS             1801..2457
                     /locus_tag="OO515_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6576036.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="OO515_002108"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="OO515_002108"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6576037.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="OO515_002109"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="OO515_002109"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6576038.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OO515_002110"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OO515_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="OO515_002111"
     CDS             5476..6393
                     /locus_tag="OO515_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6576039.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="OO515_002112"
     CDS             6472..6654
                     /locus_tag="OO515_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6576040.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="OO515_002113"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="OO515_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6576041.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="OO515_002114"
     CDS             complement(8516..9331)
                     /locus_tag="OO515_002114"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR6576042.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OO515_002115"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OO515_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6576043.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="OO515_002116"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="OO515_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6576044.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OO515_002117"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OO515_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6576045.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OO515_002118"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OO515_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6576046.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OO515_002119"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OO515_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6576047.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OO515_002120"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OO515_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6576048.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OO515_002121"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OO515_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6576049.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OO515_002122"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OO515_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6576050.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OO515_002123"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OO515_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6576051.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OO515_002124"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OO515_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6576052.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OO515_002125"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OO515_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6576053.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OO515_002126"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OO515_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6576054.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OO515_002127"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OO515_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6576055.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OO515_002128"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OO515_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6576056.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OO515_002129"
     CDS             complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OO515_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6576057.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OO515_002130"
     CDS             complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OO515_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6576058.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21825..22139
                     /gene="fliE"
                     /locus_tag="OO515_002131"
     CDS             21825..22139
                     /gene="fliE"
                     /locus_tag="OO515_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6576059.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22473..22805
                     /gene="emrE"
                     /locus_tag="OO515_002132"
     CDS             22473..22805
                     /gene="emrE"
                     /locus_tag="OO515_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6576060.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22974..23525
                     /gene="ybcL"
                     /locus_tag="OO515_002133"
     CDS             22974..23525
                     /gene="ybcL"
                     /locus_tag="OO515_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6576061.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23535..24332
                     /locus_tag="OO515_002134"
     CDS             23535..24332
                     /locus_tag="OO515_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6576062.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6111 a         6138 c         6352 g         6237 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg cgcggtacgc tggtttactt
     7501 cgcaaccgga gcacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataatttttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gagattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    19201 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac
    19261 atctcgtcga taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg
    19321 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    19381 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc
    19441 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    19501 agacgttcat cgaacaacgc cagaatatcg gcggcctgcg cgcgcttcag atgcaccaga
    19561 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcact ctgcggctcc
    19621 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    19681 aggctggcgg cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    19741 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatag
    19801 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcact
    19861 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    19921 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19981 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    20041 gacttctgcc cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag
    20101 gcggacttcc accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt
    20161 catcgcctcg gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc
    20221 caccagcagt accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg
    20281 ttgccagaac ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac
    20341 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgct tcgcgggtca gatcttcaat
    20401 ttgcttcatc tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt
    20461 caccactacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    20521 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    20581 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    20641 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    20701 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc
    20761 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt
    20821 gacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    20881 ctgaatacgg ccttcgacat cgctggcata tttcaactga gcgtcattaa gatcgcgccc
    20941 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    21001 cggcagacca gcaacggcgc tggaaaccag gtgcacaatg gcgctaattt gcccttcatc
    21061 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    21121 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    21181 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    21241 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    21301 gggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    21361 aatagcgccg ctggcttcgc tgaagcagta aggaatattc atttgcgtca gttggctgac
    21421 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    21481 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    21541 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    21601 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    21661 actcactccc gggtcagcaa actcgaaaaa ttgacgctgc cattatttgt gctttcgttt
    21721 ttttctatcc gccaataaac ccgttttttt tgttgctatt tagcgccttt gtcttattga
    21781 cttactggta agctttgcta ccagaaatta tccgggagat gaaaatgtca gcgatacagg
    21841 ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt gcgcaggaat
    21901 cactgccaca accgaccatt agttttgccg ggcagctgca cgccgcgctc gatcgcataa
    21961 gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt gaacccggcg
    22021 tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg caaatgggga
    22081 ttcaggtgcg taataagctg gtggcggctt atcaggaagt gatgtccatg caggtgtagg
    22141 ttttgtaacc tgttgttaat tacatccgat ctcaccgcgt ggggcatgga tggggcaaac
    22201 tcactcaatt tctggtttaa aatggatacc tgatcctgaa agttttctgc catccacgat
    22261 ccgtaaacgc gataacatgt tttgtcttcg ctttgctcat aggaagagtg aactcttttg
    22321 ttagcctggg gttccttctg atcattattg ttcctgcctt tatctcttgc cacgccagag
    22381 ccccctggat acacatacac caggatgaaa acggtgagtt gtgttcaaac tgtagtacaa
    22441 ttctctccag tatgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggcg
    22501 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagagggg tttacacggt
    22561 tatggccctc tgttggcaca attatttgct attgtgcatc attttggtta ttagctcaga
    22621 cactggctta tatacctaca gggattgctt atgctatctg gtcaggagtc ggtatcgttc
    22681 tgattagttt gctttcctgg ggattttttg gccaacgact ggacctgcca gccatcatag
    22741 gcatgatgtt gatttgtgcc ggtgtgttag tcattaactt gttgtcacga agcgcaccgc
    22801 attaaaaaat aatcatcctt gagataacag gaatctgatt tgtgcatatt atggctaatg
    22861 ttcggggcaa ctttataatt atgattgcat ttgcgcgatc attgcgcctt tttgcataat
    22921 gagatcaatg cacatcaaat aaaataacga taggtcacaa caaagaggtt tttatgaaaa
    22981 aacttatcgt ttcaagtgta ttggcattca taacattttc tgcgcaggcc gcagcatttc
    23041 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtattttctg
    23101 gagttgggtg tgaggggggt aatacatcgc cctcattaac ctggtctggt gctccagaag
    23161 gcaccaaaag ctttgccgta actgtgtatg atccagatgc gcctacaggc agcggatggt
    23221 ggcactggac tgttgctaat attccagcaa cggtaacata tttgcccgct gatgcaggaa
    23281 gacgtgatgg tacaaaactt ccgactggtg ctgtccaagg ccgaaatgat tttggctatg
    23341 cgggatttgg tggagcatgt cctcccaaag gagataaacc ccatcattac cagtttaaaa
    23401 tatgggctct aaaaactgat aagattcctg tggattctaa ttccagcgga gcgttagttg
    23461 gttatatgct taatgccaat aaaattgcaa ccgctgagat aatacccgtt tatgaggtaa
    23521 agtaggataa gagtatgctg gcaaaagata ggactaacct aaagattgaa gaaatacgga
    23581 tgcataaaca tcatgagatt cacagggtta agcctcttat gccagcttca tgtcgtatcc
    23641 gtcagggaaa aaaagttatc aattgggaga cgcatagtct aactgttgat aataatcaga
    23701 taatattatt tccctgtgga tatgaatttt atattgcgaa ttacccagaa gcaggtcttt
    23761 atcttgcaga aatgctttat tatcccattg atctaattga aaagttccaa aatctttatg
    23821 cgataaccga tcaaattcgt aacacgacag gtttctgctt acctcagaat tccgagttaa
    23881 tatattgttg ggagcaacta aaaacatcta tttcccgagg cttctcaacg caaattcagg
    23941 aacacttagc aatgggcgtt ctactttcat taggagcaca tcatgttaat tgtttacttt
    24001 tatcagatag taaacaatca ttaataagtc gttgttataa cctaatgcta tccgaacctg
    24061 gaacaaaatg gacagcaaac aaggtagcga gatatctcta catttctgtt tccacattgc
    24121 atcgccgtct ggcaagcgag ggagtaagtt ttcaaagtat attggacgac gtgaggttaa
    24181 ataatgcgtt gtctgctata caaacgacag taaaacccat cagcgagatt gccagggaaa
    24241 atggttacaa gtgtccttct cgttttactg aaaggttcca taatcgtttt aagataacac
    24301 caagagagct aagaaaagcg tccagagagt aaaagtattt tatgaaggag caacagcatc
    24361 gatttttatt ttggaaaata aatacggcat actcaaccta ttggagcctg aacaatccgg
    24421 tgacttctgc gctaatcggg gacgtttata agcacatata attgatacta tcttcctcca
    24481 tcacaatttc agaaatgcac gtgtgatttt tttgcatccg gcgtttggac tatgtggagt
    24541 tcaagagtgg gaaagttaca agaggttgag gggataatta aagggatgct tatgacatta
    24601 gatatccgga taggtacgga tatcgttgtt attaaatcta gttcgttatt attaaatcta
    24661 gtttatacgt taagatgcag ctacagcaga aattgccact gttaattttt tcatcgtgag
    24721 cccttttatt tgaactatta tttaaaaatg atgtcactgc actacaaata ttcatccaat
    24781 caatgagatt atttcaagat gtaagtttta gtttctcatt taaattgtga agtagatc
//
LOCUS       DAJTXQ010000055        24638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000055 DAJTXQ010000000
VERSION     DAJTXQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.055"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="OO515_002135"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="OO515_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6576063.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="OO515_002136"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="OO515_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="OO515_002137"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="OO515_002137"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6576064.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="OO515_002138"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="OO515_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6576065.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="OO515_002139"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="OO515_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6576066.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="OO515_002140"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="OO515_002140"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6576067.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="OO515_002141"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="OO515_002141"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6576068.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="OO515_002142"
     CDS             8330..10048
                     /locus_tag="OO515_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6576069.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="OO515_002143"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="OO515_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6576070.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="OO515_002144"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="OO515_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6576071.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="OO515_002145"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="OO515_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6576072.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /locus_tag="OO515_002146"
     CDS             complement(12751..13440)
                     /locus_tag="OO515_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6576073.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="OO515_002147"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="OO515_002147"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6576074.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="OO515_002148"
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="OO515_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6576075.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="OO515_002149"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="OO515_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6576076.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="OO515_002150"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="OO515_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6576077.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="OO515_002151"
     CDS             complement(20443..20718)
                     /locus_tag="OO515_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576078.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="OO515_002152"
     CDS             complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="OO515_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6576079.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23165..23482
                     /locus_tag="OO515_002153"
     CDS             23165..23482
                     /locus_tag="OO515_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6576080.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23551..23880
                     /gene="yjdP"
                     /locus_tag="OO515_002154"
     CDS             23551..23880
                     /gene="yjdP"
                     /locus_tag="OO515_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6576081.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(24027..>24638)
                     /gene="phnP"
                     /locus_tag="OO515_002155"
     CDS             complement(24027..>24638)
                     /gene="phnP"
                     /locus_tag="OO515_002155"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6576082.1"
                     /translation="ITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVG
                     DPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYL
                     LETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLAL
                     NQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
BASE COUNT         5590 a         6452 c         6715 g         5881 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc taggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggt
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    20821 ttcagtcacc gcattcataa cttatatatt ttatttatta attggctgta tatattttta
    20881 aaataaatgc ttttgggggc ttcagcgccc ccaaaagcga gtgacattaa tttggggtta
    20941 cgatattcac ccacaaatcg aaattatcca gacaggcgat aatttcttcc agtttcgcgc
    21001 cattgccaat aattttggct ttcttcgctt ttaccagatc cgccattttg gcttgcccgg
    21061 tcagcacggc gtgcagatct tcacggctga tgtagaaaga ggcgtcagct tgggattgca
    21121 gtgttttacg gtagttaagc acgctatcgt tcagcgtcag gttgaggtta tcgccattgc
    21181 tcatattgaa gttcaggctg atatttttac ccgcggcttt cgcgctatcg aggcgaacgg
    21241 acataaagtc gaacagcatt tcgaccgaca tcccgcgaat ggtgtccggg gaaccgatgg
    21301 tgccgtggct gaacttatgc accccttcgc gcagctcttt cgcgccggtc aggtagaaac
    21361 cgcgccaggt ggcggactcg gcctgatagc ccagctgttc aaagttattc gcttgcagat
    21421 tcttcgcgac ctggtcaccc gggttggcgg caatcacctg tttcagcagt tctgccgacc
    21481 agcggtaatc accttgcttg ttcgcttctt gcgccaggtt gatgacacgg gcagaaccgc
    21541 ccagcgcctg cacgtaacgt ttacccatct ccacctgacc atacggatgc aggttagccg
    21601 ggttaccgtc gtaatagcca agatagaagt tatacaccgc gcgggcgttg tggctgacag
    21661 aaccgtaata gccacggctg gcccagttat tggcaagtgc aggcggcagt ttaatcatgt
    21721 cgccgatttc attcatggtg tagccctggt tcgccaggtg cagggtctgg tcgtgaatgt
    21781 acttgatagt atcgcgatat ttaccaatat aatcattgat atgcttattg ccccagaccg
    21841 gccaggtgtg cggcataaac aggacttccg cgtcgttacc ccacatatcc agcgtttcgt
    21901 tcagatactc ggtccacttg ctggtgtcgc gggttttcgc gccgcgcaga gtgtagaagt
    21961 tgtgcagggt atgcgtggcg ttctcggcgg tacacagcgc tttcagggcc ggaatataga
    22021 agtgcatttc ggctggtgct tcgctacctg gggtcatcag gaagtcaaac tccaggccgt
    22081 cgataatcat cttctcaccg gttttggtga tggttttgtt cggtgcgatg atgctcgggt
    22141 agcccgatgc cagcgttacg ccaagaccat tgccgatgtt accctgaggg ttatgcgcca
    22201 gcagcaggcc gtaggagtaa agtgcacggc ggctcataat attacccgcc agtacgtttt
    22261 cgctgatggc ttcgtccata aagccagcag gggcgataac ctgcacttta ccggatttca
    22321 catcagcttc agagataatg cctttcacgc cgccatagtg gtcggtatgg ctgtgggtat
    22381 aaataaccgc aacaatcggt ttttgcgggc gattttggaa gtaaaggtca agggcggctt
    22441 ttgcgctcgg tggcgtcacc agcgggtcga tgacaataat cccggtatca ccttcgataa
    22501 aggtgatgtt ggagatatcc tgaccgcgaa cctggtacat tctgtcggtg actttaaaca
    22561 ggccggaaat accgtttaac tgcgactgac gccacaggct agggttaacg gtttcgggcg
    22621 cgggcgcatt gatatcaaat ttataatctt ccccgcgata atatggtttg ccattagcat
    22681 cgcgcagaat accttcatcc agcagcggag cgataaatcc acgctgagcg tcgtcaaaat
    22741 cctgacgatc gctaaacggt aatgatttgg cgtaattctg attaacctgt tgggtatatt
    22801 gagtggcggc tttcgcttcc tctttcgcgt tagcggtatt taccatcatg ccgctggcgg
    22861 cagttaacgc aataacaatt ctgctcaaac ggaataaccg agagttattc atgtaaatct
    22921 ccatttatca tttatgtcgt aaatatgtaa tccatgcaaa atacgtcatt taaagctggg
    22981 gttatcataa ttgggaattt gcgactttaa taagtggaag tgtgagctga acgatccatt
    23041 ttattaggca tttgtgctga agagggaaaa ttacgcgaat ttattatctc tgtaaataac
    23101 atgaggtgtt ttatatattt attatatata ttgtacttcg gcaaatactg agcacaattt
    23161 atcaatgcaa ccggtgtttc tttttaaaaa acgtaccgga gcaatgggcg gtcatgtcgc
    23221 tggcaccgca ggattctgcg ttgctgtgcc agccttttat tcatggtcaa tgagtgaggt
    23281 gattgataaa gacaccaggt cgtcagagcg cactgattat ccgcattatg ccagtgaagt
    23341 tgcactggct tttggttcac gagtggttgg cctcgaactg gcaaaaatga ttgtggatgc
    23401 gtggctgggc gcacagtacg aaggcggtcg tcatcaacaa cgcgtggagg cgattacggc
    23461 aatagagcag cggagaaatt gagattcatc cactacttgc atggatgagt aatgactaat
    23521 gtggatagag tttctttttg aggttggcta atgaaacgct ttccgctatt tcttttattc
    23581 accctgctca cgctgtccac cgttccggcc caggctgata ttatcgacga tactattggt
    23641 aatattcagc aggcgattaa tgacgcttat aaccctgacc gtgggcgaga ctatgaagat
    23701 tcgcgcgacg acggctggca gcgtgaagtg agcgacgatc ggcgcagaca atatgacgac
    23761 cgccgccgcc agtttgaaga tcgccgcagg cagctggacg atcgccagcg ccagcttgat
    23821 caggagcgtc ggcaactgga ggatgaagag cggagaatgg aagatgagta tgggcgatga
    23881 ggttgggtat ggagcaggca tcgcccctca ccccaatggg gagagggaga aaacgagcgc
    23941 aatattcaat attgagcaca atcggtcccc tcgcccctct ggggagaggg ttagggtgag
    24001 ggggccagcg cgcacattcc ctctcatcac gccaccccaa tctccatccc atcaaacccc
    24061 acctcaaacc ctgacggtag tgcgttttcc atcagccacg catcaaactg gtggctgata
    24121 tgggtcagaa tcacccgtgg cgagcggata acctgattca gcgcaagcac ggtatttaaa
    24181 tcacagtgat tacgcggtgc atccgcgcgc ggcgggtgac tgcaatccat caccattacc
    24241 tgcggctgat tattgcgtaa aaatttcagc gttttttccg gcaagccagc ggtgtcagac
    24301 agccacgcca cccggctgtg cgccgtttcc agtaaatagc cgaaagtgag ttttgagtgg
    24361 ttaagcggca gcggcgtgac ctgcaacccc tgcaaatcaa acaccacaaa cggttccacc
    24421 gtgtggctga aatcaagcag cccgggatgt ttaaacagat cgtcgcagcc ctgttcatcc
    24481 ggcgggccgt agatcgggat cggatcgccc acgccccagc gcagcggaaa cagcccctgg
    24541 acgtgatcca tatgataatg cgtcagcaaa aactgctgga acgatccggg agaccagcga
    24601 tcggtgagat cgtgcagccc ggcgtcgatc agggtgat
//
LOCUS       DAJTXQ010000056        24278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000056 DAJTXQ010000000
VERSION     DAJTXQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.056"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..200)
                     /locus_tag="OO515_002156"
     CDS             complement(<1..200)
                     /locus_tag="OO515_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6576083.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAV"
     gene            complement(203..1870)
                     /locus_tag="OO515_002157"
     CDS             complement(203..1870)
                     /locus_tag="OO515_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6576084.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(1867..3975)
                     /locus_tag="OO515_002158"
     CDS             complement(1867..3975)
                     /locus_tag="OO515_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6576085.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(3962..4783)
                     /locus_tag="OO515_002159"
     CDS             complement(3962..4783)
                     /locus_tag="OO515_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6576086.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO515_002160"
     CDS             complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO515_002160"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6576087.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO515_002161"
     CDS             complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO515_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6576088.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO515_002162"
     CDS             complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO515_002162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6576089.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO515_002163"
     CDS             complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO515_002163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6576090.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO515_002164"
     CDS             complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO515_002164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6576091.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO515_002165"
     CDS             complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO515_002165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6576092.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO515_002166"
     CDS             complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO515_002166"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6576093.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO515_002167"
     CDS             complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO515_002167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6576094.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO515_002168"
     CDS             complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO515_002168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6576095.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO515_002169"
     CDS             complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO515_002169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6576096.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO515_002170"
     CDS             complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO515_002170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6576097.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO515_002171"
     CDS             complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO515_002171"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6576098.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO515_002172"
     CDS             complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO515_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6576099.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO515_002173"
     CDS             complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO515_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6576100.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO515_002174"
     CDS             complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO515_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6576101.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20256..21248
                     /gene="asnA"
                     /locus_tag="OO515_002175"
     CDS             20256..21248
                     /gene="asnA"
                     /locus_tag="OO515_002175"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6576102.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO515_002176"
     CDS             complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO515_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6576103.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO515_002177"
     CDS             complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO515_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6576104.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6003 a         6312 c         5775 g         6188 t
ORIGIN      
        1 acagcgtaac gtcctgtttg atgtagatgt cttgctatca cacctaaatg cgagggcaga
       61 cctaaggtag cgaccacctt gcggttgcca tacacctcat ccaacagctg cttaggacta
      121 acaatccctt gataaacgcc tgcccgtgca atagtgctat aaatcagctc attcgagtac
      181 ggaacaggaa agtttctcat ggctaacctg cttgtttaaa taagctcgcc atatccacta
      241 ttaccccttt cccttttaac cgttcatgca tggttttttc aggttggcgt tgtgaataga
      301 tataacgtaa atccgtatca ggcaagctat cccagtcgct gggcttgact acctttatag
      361 ccgaaacctt tttactcttg gagggctttt ctagttctga cactaccgtt tcgccttcca
      421 tcaaccactg caaaacaaga ggcagtaact tttgtcttgt catcgttgga ttctggctaa
      481 acgctttttt aatagtggga attaacaggc ttgaatcgta atcctctttc agcatcagat
      541 ataaatgacg ctgatcttcg gtatctaact cttgaagggc tttttcttct ggtgtttgtt
      601 cttgtatcgc tgcgatatct agctgaagtt ggattaaccg tttatcaatc tcgggaacga
      661 ctagatcaga ataacgagca atgcgttctg ggatacccga gcgtaatgcc tctagcatgg
      721 ggtgcacagg ctttaactca tcttgataca cttgccgcaa taaaccagcg gtaatacgct
      781 cattgcctaa agctagcgca cggagctgag cgagtacaaa aagttttact acaatgtcca
      841 tcactccttg gcttagctca taccacacat cacggacctc atccgataac agcgcatctt
      901 tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa agcgatccac tcttgattgg
      961 gctttccacg ttgcgtttgt tgtataggat cccagaatat agctccaaac cctgcccctc
     1021 tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc tttaggggta ccaatcaaca
     1081 tcactggtac gccaataata ttcaccatcg tcacaaaaaa gttcagcatc tcttgagatc
     1141 cacccgaacg agagcggctt aaatgctgaa tttcatcaat aaccaacaac cctaaagcat
     1201 gtgcattggc tatttgcgac atcaaagcca acatggtttc tataccatga cgttttaagc
     1261 cataacgacg ctcatagttc gagcccaagg ctcgatccaa cgctctgaaa aaattcaagc
     1321 agatttcttt tagcgaacca ttatgcgagc agtctatttt caaatacacc acctgctcta
     1381 cattgagttc acgatggtaa atcacctgag gatacgtggc tagaatacga tgaagagagg
     1441 tcgtcttccc actaccagaa caaccaatta acaataagct ttgtgccgta gatcgtgcct
     1501 cctcaaagcg aaatgtctcc aactctcccg tttgaacacg ctcataacca ttttgtaaat
     1561 gcttttgtaa atctcctgtt ttaggatttc tgcctacgta gccacctcga atcatgaccg
     1621 aaatacgctc acttagtagc aaatgcgtac ctaatggctg aaaatagtca tctggaatac
     1681 gacaaatggt atgagctctg ataacacggg acttttgcaa gtcagaggaa gtaagctgta
     1741 aagaggattt cagtgatgca gcactattca ctgactcttg taatggtggt aaggcctcga
     1801 taaaaggatt atcacgataa gcctctaccc ccgtatcacg ataaactgct tgaatccggg
     1861 tagcactcat gactcatcct tttctggtgg atcctgaaat aattcaggca cgtatgtggg
     1921 taggctgtaa tcttcttgat catccgcttc cactgcgttg aaaggaataa ctttagcctc
     1981 atcacctgaa gagcttggct tcaaatgctc cgcacgtttt ttacgctccg aggtcacggc
     2041 ttctttttta ttagttttaa tctgcttaat gcgtgttgat ttgggctcag tagtactggg
     2101 cgttaactta ttcgctttct gaatggtttg ctgaataaac gcctcaagct ccctgcgttt
     2161 agttaactca tcctgcttcg cattggcttt attgtgtttt tcttgtgctt gtatatccca
     2221 aacctcccaa aatgagagac ctttaaactg ccgactacgt tccgtcagat tacagcgcca
     2281 aaatacacgg ctgccaactt gcggaaacaa ataaatcgta tcaaccagca ctgggtcata
     2341 agccgcttct aaatgttgag gtctagctat atcagtgctc cgctgcaacc aaccctcacg
     2401 cagaatctct gaccccgagt aatacaaacc ccacaaatta acgccaaatg aagaaataga
     2461 gacctttcgg cgaggcagta acgctactcg caactgctct tgctccacag cccttaaact
     2521 acctgtacga tgctgcatac cccattgcca tagctggaca ggaatagacg gtaaatctgt
     2581 aggaaaatca gcatctcgat cgtatttatc catcaccaga tggttatttc tgaataagat
     2641 cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga gatgcatcta acctatagtc
     2701 tgtttcacca tggcttttga tccgactgcc ctctacaatg ccaggtgcaa aggacttaaa
     2761 ctcggcttgt agtgttctaa aagtgctttc cactatgcct ttagcatcgc cacgtctagg
     2821 tggagcactt tccactcgca cattaaaact agaaactaag gcttcgacct gatgactcat
     2881 taattcgcca cggtccgcta gcaacacatc tggcaaacct acacacggcc agtctgagct
     2941 actaatctca atatcatgct gggcacaaat ggccgttttg tcagagcaag catttacaaa
     3001 agcctgcatc gccaccacat aagacggatt ttcaaagccg atataaaagc ccgtgatcat
     3061 ccgactaaac acatcaatca caatgtaaag cgttggtctt cctatgattt tttggcgatc
     3121 atgatgatcc actaaataaa tatcagcaat cgtggcatca atctcataac gactcccagg
     3181 gcctaacgcc tgagaagtgg ctgtactact taagggtcgt acgtcttttt tatatacccc
     3241 tgctttaact ctagacttta agcgctgagc tttagggtat tctcgatcat aaaaataacg
     3301 aaactgacgt agtgttgggt aatcctcttg gggaatgcga ggaaaatact gagcaaacaa
     3361 gtccacaaat cgtctatagg caacggtggt ctttgtacct ttttgattta acaggtgctt
     3421 ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt accttggttc cttcaccctt
     3481 accatattct ctggctcggc caatctttgc tgttcctgtc gctgaacgtc tttcccctgg
     3541 tgcaccgctg tttttgtagt caggaattaa tgcattaggc gtttgaccac gctgccagta
     3601 acggcgtaac aacttataaa ctgtagcctt agtaacctta tgttcttgga ccacatgctc
     3661 aacgagttcg cttctgactt tagggtcgaa acgatcctta ctattaataa taggaagaat
     3721 ttttcgataa tcctcctcgc gcttctggaa gctgacagaa tctacagacg gctcttctaa
     3781 atcaagatgt acataagggt catcagttct aactaagcgg ccttcatcta aatactgcat
     3841 tagcaacaac tcagccctag cttgtggaac tcctttatca gcgcttattt gcatccagac
     3901 aacttggcca tcctctatag ccaaaatgcg atacggatca ttatcaaata gcacaacctc
     3961 attaatttgc cacatagcgc aactcctcca tattcactac ttggctaata cagagatctg
     4021 cacacttatt tgccctgaaa gacttataaa tattgaactt aataaaacca tttgcggtta
     4081 aggctcgtat ctcactcaat gttttgccta actccaaatc ataagcaata tcaacctgct
     4141 tacagacatt gataatgttt tcatctcctt tttcttgcag gatatgggcc aatggggata
     4201 gttgtgctaa aagctccgca gaaacttctt ctgttttcac tgaataaagc cattcaatat
     4261 tttcttttac tacgggattt atttctttat cagtaaaaat gaaccaagga atttgctttt
     4321 gctgccaata gcgacgctct agttctagtt tttctaaggt acgctcgtct tgtaaggctg
     4381 ctgcaggttt gacttgaata gcaaactgct caaaaggacc atctttgcag tccactaaaa
     4441 aatcagtaga cataacctga tctacaccac gaataacagg atgcttaata ccactatcta
     4501 ttgcaatctg cctggtatca ctaggtaata aggggaactg ctcgcgtata tctagcacgc
     4561 tgctctccca ctcaagactg agaaaaacag caagctctaa gtcagatagc aaatgatgga
     4621 ctcgtcccgt cttatgagaa taaatacggt gggaacgacc tgaagaagga acttcttgta
     4681 ctgttagcca tggaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac
     4741 gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg
     4801 ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact
     4861 ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacagcc
     4921 cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaggt tacgcggctg
     4981 gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca gcggaacggt
     5041 gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt gcatgttatc
     5101 gctacttaca aaacccgcat cctgatcggc gaagacatac aattggccgc cacgcgcgcg
     5161 aacttcttca atgttggatt tcagtttttc cagcaattcg ttgttcggtg caacgacgat
     5221 aaccggcata tcggcatcaa taagcgccag cggaccgtgt ttcagttcac cagcagcgta
     5281 ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca gtgcgattgg
     5341 gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat cttctgccag
     5401 agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca gcgcctgcag
     5461 accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag acagcttcgc
     5521 caccagcatc aacagcacag ttaactgagt ggtgaatgct ttagtggacg ccacgccgat
     5581 ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag aagaacccgg
     5641 aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac gcaggcccgc
     5701 cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt tacgacgcac
     5761 ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa tacctgccag
     5821 cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac aggcgaggat
     5881 ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct cgcttaaatc
     5941 aacctgaccg tggctgatgc gcccggtaag ggtgtttttt atcgcgttcg gctgttcgta
     6001 gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat attgcagatt
     6061 ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt ttaccgaacg
     6121 gcgagtgatt tccgcaatat cgccctcttc aaggaagata aagcgacggg tcaccggcaa
     6181 cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa tcaccagcgg
     6241 actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga tcactgtacc
     6301 gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag tcccgccttg
     6361 tttcagctcc cagttcacca gatgggcaat cacttcggtg tcggtttcag aaacgaaggt
     6421 atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga tgccgttatg
     6481 caccaccaca atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag gttcaccgtg
     6541 ggtcgcccag cgagtgtgag caataccggt gccgccatcc agaggatgtt cttccgctgc
     6601 ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac cttccgcatc
     6661 aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta aaccttcaag
     6721 aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca tagtttttga
     6781 ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg ccccgagcct
     6841 tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta tgttatctcc
     6901 tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg acgacgccag
     6961 ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc gacattacgc
     7021 gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg ggccaccagc
     7081 tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt attcgcacca
     7141 tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat ttccgcatcg
     7201 cccaggtaag tcagatgccc gactttcgag cctttaccca gacgcgcttt tttcatctca
     7261 acgaagttac caacgtgtgc accttccagc aactcagcac caggacgcaa acgagcaaac
     7321 gggccaatag tacaggccgc tgccagattc gcgtcttcca caacggtata cggactgatt
     7381 tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat tttcacgcga
     7441 tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac atcgcgcccg
     7501 tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac gcctgctaac
     7561 agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag ttgcaggcgg
     7621 ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat ttcacgccct
     7681 tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc attattgttg
     7741 gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat gccggtgttg
     7801 atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat gccggtaact
     7861 ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt caccgtcagc
     7921 agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc gacagagatc
     7981 agcggcacgt cgccgtagag cattaaaatg tcttcatcat cggcaaagaa aggtgcggcc
     8041 tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt aaggttgtcg
     8101 tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaagtg aacgtgcgct
     8161 gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc ggcaagggta
     8221 tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc aaggatcact
     8281 acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg aatttaaacc
     8341 gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga taaattgttc
     8401 aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac gccttttgtc
     8461 tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt gctcttattt
     8521 cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct atgtgaacgc
     8581 tcttcaggac ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg cttttgtgct
     8641 tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc tgcgcgatcg
     8701 ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag ctgctaatgt
     8761 gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc tggccgcgaa
     8821 ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg ccgccagaca
     8881 gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca ggcttaatgg
     8941 cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta cccgttacct
     9001 ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg tccaggtggt
     9061 aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt tttccacagc
     9121 ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt attcgccttc
     9181 catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac ccggagaacc
     9241 ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct tacgagcacg
     9301 cgctaccacc agtttgtctt cttcagacag ttcatccata cccaagatgg cgatgatgtc
     9361 tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt cgtagtgttc
     9421 ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt caacggccgg
     9481 gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa ggtgcgcaaa
     9541 ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg cctgtacgga
     9601 ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca tctcttccgc
     9661 cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg atacttccgt
     9721 accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac gaccttcgtc
     9781 acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt ttcccggcgg
     9841 ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt cggtcatttc
     9901 gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca cagagtaacc
     9961 ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt tacctacacc
    10021 cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca ggtcgataac
    10081 tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg aaggtgctgc
    10141 gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga ccggttcacc
    10201 cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga tcgggtgttc
    10261 gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg cgatggtacg
    10321 cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat taccattttg
    10381 cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga cgtcaactac
    10441 ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac gaaataacct
    10501 gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat gctggcctga
    10561 cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt gtcggtcgcg
    10621 gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac cacgccctga
    10681 taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt cggatcgggt
    10741 tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc cggtaacggc
    10801 agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt gctgacaatg
    10861 taaagcttgt ccagacggcc ttcgtcgtaa gcctgcaaca tcacttttac cggaccgatc
    10921 agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac attgccgccc
    10981 acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca ttgaacgctt
    11041 ttgtcggtcc aggtcttcat ttccgccagt agttttttga acaggttaat gttcaaacca
    11101 ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac gtcgcggtct
    11161 tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat cactttgcgc
    11221 atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg cattttggaa
    11281 gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct tgcgatctta
    11341 ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg cctgccctaa
    11401 ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg atgcctttca
    11461 gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc atcaacggag
    11521 cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt ttcgacagtt
    11581 caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc tgcgcaacgg
    11641 acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg tggtcaagct
    11701 gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc agttcacgat
    11761 actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg gtctgtgctg
    11821 caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata ccggcgttga
    11881 acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg gtcggaacga
    11941 acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga gaaccggttt
    12001 tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca cgtgcagcac
    12061 gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca cgtcctggcg
    12121 gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac aggtcatcgt
    12181 aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg caaccggcat
    12241 acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca acgatggtgt
    12301 tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg gacgctttct
    12361 ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg atggcatcga
    12421 tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc tgaccacgac
    12481 cgattgggat catggagtca acggctttat aaccggtctg taccggctga tctacggact
    12541 gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg tgatccagcg
    12601 gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc aggccacggc
    12661 caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg gcaaggtcag
    12721 cgtacggacc cataacaacc gcacctacag agtcgcgctc aaggttcagt gcgatagcgt
    12781 aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg ccgtgaatgc
    12841 ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca ctcacaacat
    12901 tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc agttgcatgc
    12961 tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg cgtacgctgc
    13021 catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac ttatcgattt
    13081 tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa attttcgcga
    13141 gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta gcctcactca
    13201 cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga cgaccatttt
    13261 cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca caaactgcga
    13321 taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct gccatttgtt
    13381 cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct acactttggt
    13441 gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta ataaattcag
    13501 acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt cgctgttagc
    13561 agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca ggatagcaac
    13621 ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg cctgcgcctg
    13681 ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca gaatctgcga
    13741 gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt ttttcagctg
    13801 gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg cggaagcaag
    13861 gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg gccatacgta
    13921 cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga ttgttgcgtt
    13981 aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct ttaaataaaa
    14041 gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca gcgatcatcg
    14101 ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga ggaatcagat
    14161 caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata ccgatcgcag
    14221 caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga tccatattca
    14281 ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt ggtaaattaa
    14341 tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat gaaggcttgc
    14401 agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca ctgtgaccac
    14461 cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat gttaccgaac
    14521 agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag gattaagttg
    14581 acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt gaagccgccg
    14641 atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag tgccatagac
    14701 agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc cagtacatgt
    14761 tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat caggaatacc
    14821 cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg gtacatgtct
    14881 ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa cttacctggc
    14941 acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa cagacccagc
    15001 accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg ggggttttgt
    15061 ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg gtgtcctatg
    15121 taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg ttgttaatta
    15181 cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc agcggcaaga
    15241 ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc agaactttga
    15301 aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta tacgcctggt
    15361 gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg cccccgctta
    15421 ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt gctatcacca
    15481 ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc gacacagaca
    15541 tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa actttcttta
    15601 aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc gtgatttcaa
    15661 acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca tacaattgcg
    15721 acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa acactagtga
    15781 taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagtatattt atttacttgg
    15841 caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact gttttaataa
    15901 atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt aacagccaat
    15961 gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact tattttctgc
    16021 ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc gttaaatgta
    16081 accagttata tcagtagaaa acttggttgt tgttaacagt ctaaccggtc aattttttat
    16141 gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg acgttcgcca
    16201 tccagagctg gaacctgaag tttaaccact gattcgacct gatattcttc gggcaacaaa
    16261 gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg ctcaccagga
    16321 agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca
    16381 tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggctc aatattctcc
    16441 agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac
    16501 agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag tcctggtccg
    16561 gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga
    16621 atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttgttc
    16681 catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa
    16741 atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga
    16801 tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc gctacgacgc
    16861 agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg cgtgacgcca
    16921 gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt ggcgatcact
    16981 tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg
    17041 ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc
    17101 tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa cggcgtcagc
    17161 gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc actggcttca
    17221 cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt
    17281 acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag
    17341 cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt caaacgcaga
    17401 tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa catacgatac
    17461 ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata cgcctgagaa
    17521 cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag accgaccagc
    17581 aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac
    17641 agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc gaagaagtca
    17701 tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catggagcgg
    17761 acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttcg
    17821 ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc
    17881 atgactttgt cttcgatcga tgggcagtag cgtgggccga caccttcgat cacccctgcg
    17941 tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatga
    18001 gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa cgagaatacc
    18061 ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt tcgagcatca
    18121 atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc acgcaaacgg
    18181 cgagaaagcg gaatggacgg cggatcacca gcacgaccac cgctgtaatt atccagaccg
    18241 atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt ggcacggaac
    18301 ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat aagatcttca
    18361 accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga
    18421 cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc tttgcttgcg
    18481 tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag accgccgagt
    18541 gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc
    18601 atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc
    18661 gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga
    18721 tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg
    18781 gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa
    18841 agaagatcta tttatttaga gatctgttct attgtgatct cttattagga tcgcactacc
    18901 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
    18961 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
    19021 aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct tcctgacaga
    19081 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
    19141 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
    19201 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
    19261 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
    19321 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
    19381 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
    19441 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
    19501 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
    19561 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
    19621 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
    19681 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
    19741 catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg tagtggcctg
    19801 ttgtgtaata ggcttcagtg acttcagcaa ggctttccag ctttgccagc gcggaagggt
    19861 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
    19921 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
    19981 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
    20041 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
    20101 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
    20161 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtttgtcgg tttttgttgc
    20221 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
    20281 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
    20341 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
    20401 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
    20461 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
    20521 gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttctccgt
    20581 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
    20641 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
    20701 ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc cacttcgtac
    20761 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
    20821 tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg agcgatggtc
    20881 atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggtc
    20941 atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat gcgtttgagc
    21001 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
    21061 gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc
    21121 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
    21181 atatcggcca ggttcagtgt ggtgtatggc cagctgctgt tcgcgagaac gtcccttctc
    21241 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
    21301 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
    21361 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
    21421 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
    21481 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
    21541 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgg gggcctgaaa gctcataacg
    21601 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
    21661 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
    21721 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
    21781 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
    21841 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
    21901 ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
    21961 aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
    22021 tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca gttccggctg
    22081 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
    22141 ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa tcggttcaag
    22201 ttgtccgctc agcgtcatgc gttcttgaac ttcactcaac agttgttcgc gttcttcttc
    22261 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
    22321 aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact gccgggcttg
    22381 ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
    22441 aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
    22501 cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc ggggaacatc
    22561 atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca gctgcggtga
    22621 ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
    22681 attaagcgta tccagcgtta gcattgttgt gcctggtgaa tctgtgcacc gacatcctgt
    22741 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
    22801 ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt
    22861 tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
    22921 acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt ctgggcaaag
    22981 ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg ttccagcgcg
    23041 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
    23101 agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
    23161 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
    23221 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggca
    23281 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
    23341 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
    23401 aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga gacataaggc
    23461 gcatccggta atttatccag ttgcaggcgc agcataaaaa tcagctcaaa tacatgatcg
    23521 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
    23581 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
    23641 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
    23701 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
    23761 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
    23821 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
    23881 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
    23941 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
    24001 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
    24061 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
    24121 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
    24181 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
    24241 atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DAJTXQ010000057        23952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000057 DAJTXQ010000000
VERSION     DAJTXQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.057"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(39..401)
                     /locus_tag="OO515_002178"
     CDS             complement(39..401)
                     /locus_tag="OO515_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6576105.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="OO515_002179"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="OO515_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6576106.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="OO515_002180"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="OO515_002180"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6576107.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="OO515_002181"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="OO515_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6576108.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /locus_tag="OO515_002182"
     CDS             complement(4828..5037)
                     /locus_tag="OO515_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576109.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="OO515_002183"
     CDS             5322..5684
                     /locus_tag="OO515_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6576110.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6227..6905
                     /locus_tag="OO515_002184"
                     /pseudo
     CDS             6227..6905
                     /locus_tag="OO515_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6949..11799
                     /locus_tag="OO515_002185"
     CDS             6949..11799
                     /locus_tag="OO515_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6576111.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12167..13822
                     /gene="lldP"
                     /locus_tag="OO515_002186"
     CDS             12167..13822
                     /gene="lldP"
                     /locus_tag="OO515_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6576112.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13822..14598
                     /gene="lldR"
                     /locus_tag="OO515_002187"
     CDS             13822..14598
                     /gene="lldR"
                     /locus_tag="OO515_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6576113.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14595..15785
                     /gene="lldD"
                     /locus_tag="OO515_002188"
     CDS             14595..15785
                     /gene="lldD"
                     /locus_tag="OO515_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6576114.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15981..16454
                     /gene="trmL"
                     /locus_tag="OO515_002189"
     CDS             15981..16454
                     /gene="trmL"
                     /locus_tag="OO515_002189"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6576115.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OO515_002190"
     CDS             complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="OO515_002190"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6576116.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OO515_002191"
     CDS             complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="OO515_002191"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6576117.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OO515_002192"
     CDS             complement(18427..18894)
                     /gene="secB"
                     /locus_tag="OO515_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6576118.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OO515_002193"
     CDS             complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="OO515_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6576119.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19350..19781)
                     /locus_tag="OO515_002194"
     CDS             complement(19350..19781)
                     /locus_tag="OO515_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6576120.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20026..21570
                     /gene="gpmM"
                     /locus_tag="OO515_002195"
                     /gene_synonym="pgmI"
     CDS             20026..21570
                     /gene="gpmM"
                     /locus_tag="OO515_002195"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6576121.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21580..22863
                     /gene="envC"
                     /locus_tag="OO515_002196"
     CDS             21580..22863
                     /gene="envC"
                     /locus_tag="OO515_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6576122.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22867..23826
                     /locus_tag="OO515_002197"
     CDS             22867..23826
                     /locus_tag="OO515_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6576123.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6044 a         6108 c         6212 g         5588 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata tatagaagat
     5881 atcatttatt aattaacaag cagttacgtc tctttttttc ggatttaatt gtgaaatttt
     5941 aatcatcata ttcatacaat aaattatctg taaatttttt cccagacatg gtttctttca
     6001 tatttgacat ttattaataa tcatcattag aatccacgaa aataatatat tcatacaaac
     6061 acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa
     6121 tcgacacaca tcactgtcat taatgtaaag caacatagat caccaataat acaatggaca
     6181 actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa
     6241 gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat
     6301 tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacatt aaaattgatc
     6361 tacttgaaaa acaacaagca cagctaaaga gtcaactgga aactattcaa aaacaacaaa
     6421 ctggcataat aagcagtaca aagactttaa ctcacgtaat taaatccgtt aaagatcaac
     6481 aaaacacttt tatttttacc gagtttaacc cagcaaaaac gaagtatttc atcctgaaca
     6541 atggttcggt ggctttagcg ggtcgagtgt tatccattga cgccacagag aatggcagtg
     6601 ttattcatat ttcactggtc aacttattaa gtacacctat ctcaaatatt ggttttaatg
     6661 cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg caacaattac
     6721 ttttcaacac atcaatgaaa tccacattga aattattacc aggtcaatgg caagacatta
     6781 atttgaccct gaagggtgta tcgcccaata acctgggata tctgaagtta gccatcaaca
     6841 tggaaaatat tcagttcgac aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa
     6901 aataaatgca gttagtacgc ttataacaag gaatagcgag catcattaat gaacaaaata
     6961 tttaaagtta tctggaaccc tgcgacaggg aattatactg ttaccagcga aacggcaaaa
     7021 agccgtggca agaaatctgg gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga
     7081 atgttgtcgt cgtttggggc attggcgaat gccgggaatg acaacggcca gggtgttgat
     7141 tacggtagtg gatcagctgg cgacagctgg gttgctatag gcaaaggggc gaaagcaaat
     7201 acttttatga acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc
     7261 caatatagct ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt
     7321 ggggttagtg caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca
     7381 ttgggccaat actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt
     7441 gctatggggg actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact
     7501 aaagctactg agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat
     7561 tcaatggcgc tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt
     7621 agcagtgtag ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca
     7681 gcgaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     7741 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     7801 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     7861 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     7921 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     7981 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     8041 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     8101 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     8161 ctttatgcca ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt
     8221 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
     8281 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
     8341 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
     8401 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
     8461 gacgatgtcg aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat
     8521 acagcaagta ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac
     8581 cttcaggctg atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc
     8641 caggatacca ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact
     8701 gatgctgtta acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc
     8761 aatatcgcca ataacacttc caatattgcc actaacacca ccaacatctc taatctgact
     8821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     8881 acggcagctc atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg
     8941 acgactggca gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg
     9001 gcgacgaata ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg
     9061 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca
     9121 gctcatggca acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca
     9181 accagttccg atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc
     9241 tacttcggcg gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa
     9301 atcggtgaaa caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt
     9361 agcacgtctc tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa
     9421 cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac
     9481 tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat
     9541 gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc
     9601 attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt
     9661 gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac
     9721 ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc
     9781 agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg
     9841 ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt
     9901 gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg
     9961 ctgtgggatg agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa
    10021 gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg
    10081 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat
    10141 cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac
    10201 gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct
    10261 acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac
    10321 agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg
    10381 attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac
    10441 accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa ataccgccaa
    10501 atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg ccagttgcag
    10561 aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa ctcaacggca
    10621 gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat
    10681 ggtaatgcag gtattggtat cggtttgaac actctggttc tagctgatgc gatcaacggc
    10741 attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt
    10801 tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag
    10861 aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc
    10921 gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt cactgatgag
    10981 cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac taatctggat
    11041 actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg tagcaccaag
    11101 tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga tagcgttgct
    11161 attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac cggttccgtt
    11221 gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg tatcaccaac
    11281 gtagctgccg gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa
    11341 gcaggcggcg tgcgctacga caccaaagct gatggttcta tcgactatag caatatcacc
    11401 ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
    11461 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
    11521 gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
    11581 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc gggtgccagc
    11641 atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg
    11701 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
    11761 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
    11821 ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgcccc
    11881 gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct
    11941 ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
    12001 ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca
    12061 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
    12121 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
    12181 acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat
    12241 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
    12301 gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
    12361 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
    12421 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
    12481 gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt
    12541 cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
    12601 gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac
    12661 cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg
    12721 catcgacagc tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat
    12781 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc
    12841 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
    12901 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
    12961 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcaaagat
    13021 tgatatgacg ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
    13081 gttcctgttc ctgacagcta ccgtaacact gtggagcatc ccgccgttta aagccctgtt
    13141 cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
    13201 actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
    13261 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
    13321 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
    13381 ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc
    13441 cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt
    13501 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
    13561 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
    13621 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
    13681 tatcgcctgt gcggcggtag gcttggtggg caaagagtct gatttgttcc gctttactgt
    13741 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
    13801 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
    13861 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct
    13921 gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactgcgcga ggcgctggca
    13981 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
    14041 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
    14101 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
    14161 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
    14221 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
    14281 attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc
    14341 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
    14401 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
    14461 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
    14521 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
    14581 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
    14641 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
    14701 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
    14761 ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
    14821 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
    14881 cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag
    14941 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
    15001 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
    15061 tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa
    15121 atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg
    15181 gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc aaaccgaccg
    15241 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag
    15301 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
    15361 cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt tctaaccacg
    15421 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
    15481 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
    15541 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc
    15601 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga
    15661 aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg
    15721 tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg
    15781 catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg accgttcggc
    15841 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
    15901 cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct gctatcctgc
    15961 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
    16021 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
    16081 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
    16141 ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa aatccccagc
    16201 gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
    16261 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
    16321 tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaatctgt
    16381 ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat ccgggagcgt
    16441 tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac
    16501 aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc
    16561 catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc
    16621 ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
    16681 gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg
    16741 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
    16801 aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc
    16861 gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga
    16921 aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc
    16981 gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt
    17041 atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
    17101 ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat
    17161 tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt
    17221 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    17281 ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    17341 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc
    17401 cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg
    17461 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    17521 ctacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    17581 cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    17641 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc agccccgcca
    17701 tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atttcagcca
    17761 gcccacgggt gatcagtgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
    17821 ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg atgaaatccg
    17881 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga tcatcggcaa
    17941 aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc agttctttcg
    18001 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    18061 cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg
    18121 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    18181 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    18241 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    18301 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    18361 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    18421 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    18481 catgaacagc gcatcgaagt taatcggcgc aaggttcagt tgcgggaatg taccgcggga
    18541 taccacgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    18601 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    18661 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    18721 cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc
    18781 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt
    18841 ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag
    18901 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
    18961 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca
    19021 atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc
    19081 tcttcacgct tggcggcatt gccatcgatc ggcaactcct gaaaactcac gcccttgctg
    19141 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    19201 ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag ggcggctgca
    19261 aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac
    19321 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg caagttttcg
    19381 ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc agctttcgtc
    19441 agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg
    19501 tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg caacagattg
    19561 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    19621 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    19681 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    19741 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    19801 cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag
    19861 cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc
    19921 ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt
    19981 agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa
    20041 aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca gcaggataac
    20101 gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg tccgcatacc
    20161 ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa
    20221 gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac
    20281 gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa
    20341 gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg cggcgtacac
    20401 agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa
    20461 atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg
    20521 ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt
    20581 ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac
    20641 ctgctgactc tggcgcaggg tgagtttcag gccgatactg ccgttgctgg tttgcaggca
    20701 gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt
    20761 cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac
    20821 cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag
    20881 aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg
    20941 gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac
    21001 aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac
    21061 ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg
    21121 gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg
    21181 gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta
    21241 ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg
    21301 gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac
    21361 ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac
    21421 ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc aggcggcaaa
    21481 ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg
    21541 actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat
    21601 accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc
    21661 gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa
    21721 ctcaaatcta ttcaggccga tatcgccaca aaagagcgcg cggtacgcca aaagcaacaa
    21781 caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc
    21841 cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg
    21901 aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgca
    21961 caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat tctcagcggt
    22021 gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga
    22081 caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa
    22141 ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg
    22201 aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga atcttccatc
    22261 cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct gcgtaacagt
    22321 attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg tgaggctcag
    22381 gcagttcgcg acaggcagaa agaagcgacg cgcaaaggca ccacctacaa accgaccgaa
    22441 agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc
    22501 tggccagttc gtggccctac gctgcatcgc tatggcgagc agctacaggg tgaattacgc
    22561 tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat tgccgacggt
    22621 cgggtgattc tggctgactg gctgcaaggc tacggtctgg tggtggtggt tgagcacggt
    22681 aaaggcgata tgagtcttta cggctataat caaagcgcgc tggtgagcgt tggttcgcag
    22741 gttcgtgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg cagaccttca
    22801 ctctatttcg aaattcgccg ccagggtcag gccgtcaatc cacagccgtg gttgggaaga
    22861 taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc
    22921 tcccctgtaa ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgaac
    22981 aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt acccgattca
    23041 ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt gttgattcat
    23101 ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctgcg cccggagatg
    23161 agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg
    23221 gggatcaata accacatggg cagcaagatg acctctaacc tgtttggtat gcagaaagtg
    23281 atgcaggcgc tggagcgtta taatctttac ttcctcgaca gcgtaaccat tggtaatacc
    23341 caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa ggtgttcctc
    23401 gacgattcac agaacgaagc ggacatccgt gtgcaattca atcgcgcaat tgacctagcg
    23461 cgtcgcaacg gatcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgttg
    23521 caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg
    23581 aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga cacaccgcgt
    23641 aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct
    23701 aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc
    23761 cagcatcagt ggcaaggctg gggcaaacag cctgaagcgg cgaagcttaa cgctagcgta
    23821 aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata
    23881 agcggaaaga ctacagtaac gcctgataac ccacctttac gctgcgcaca ttggtaatca
    23941 ggcgcttata ca
//
LOCUS       DAJTXQ010000058        23846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000058 DAJTXQ010000000
VERSION     DAJTXQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.058"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO515_002198"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO515_002198"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6576124.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="OO515_002199"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="OO515_002199"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6576125.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO515_002200"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO515_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6576126.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO515_002201"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO515_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6576127.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO515_002202"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO515_002202"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6576128.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO515_002203"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO515_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6576129.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15079)
                     /locus_tag="OO515_002204"
     CDS             complement(14756..15079)
                     /locus_tag="OO515_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6576130.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15064..15471)
                     /locus_tag="OO515_002205"
     CDS             complement(15064..15471)
                     /locus_tag="OO515_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6576131.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15468..16031)
                     /locus_tag="OO515_002206"
     CDS             complement(15468..16031)
                     /locus_tag="OO515_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6576132.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16022..16492)
                     /locus_tag="OO515_002207"
     CDS             complement(16022..16492)
                     /locus_tag="OO515_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6576133.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16677..17471)
                     /gene="thyA"
                     /locus_tag="OO515_002208"
     CDS             complement(16677..17471)
                     /gene="thyA"
                     /locus_tag="OO515_002208"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6576134.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17478..18353)
                     /gene="lgt"
                     /locus_tag="OO515_002209"
     CDS             complement(17478..18353)
                     /gene="lgt"
                     /locus_tag="OO515_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6576135.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18504..20750)
                     /gene="ptsP"
                     /locus_tag="OO515_002210"
     CDS             complement(18504..20750)
                     /gene="ptsP"
                     /locus_tag="OO515_002210"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6576136.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20763..21293)
                     /gene="rppH"
                     /locus_tag="OO515_002211"
     CDS             complement(20763..21293)
                     /gene="rppH"
                     /locus_tag="OO515_002211"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6576137.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            21978..22667
                     /gene="mutH"
                     /locus_tag="OO515_002212"
     CDS             21978..22667
                     /gene="mutH"
                     /locus_tag="OO515_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6576138.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22736..23449
                     /locus_tag="OO515_002213"
     CDS             22736..23449
                     /locus_tag="OO515_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6576139.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23587..23805
                     /gene="ygdR"
                     /locus_tag="OO515_002214"
     CDS             23587..23805
                     /gene="ygdR"
                     /locus_tag="OO515_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6576140.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5699 a         6526 c         5960 g         5661 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctactctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat tttaccgagc
      301 ttattcagca ctgctttacc aaacttaagg ctgtcagcaa tggtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctgg cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacctg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatc cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt cgaataatca tgcaaagggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     2041 gtcgtcaact ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actggccatc aaactgaaag
     2161 ctgagaagat gattggtttt tgctcttcac agggcgtcac taatgacgac ggtgatattg
     2221 tctcagaact tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgtcgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 tcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc gccggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tatccccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactatc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcga ccttcgtacc aacgagagtg
     3361 cgaatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     3421 agccacgcca aatggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagcc gtttttcaat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagttcccgt
     3781 caggcggctt agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gctgccgcca ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaaggattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 cgatcatagt cgtaatcagc aatgcgatgg cgcagatagc gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acagccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actatcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaaga caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttctggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcac cagatcacgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttgtc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtgacggg aagctagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgacc tcttccagca ggtgttcgta caatggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata aagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttttggctgc cggaaatctg cgacaggata gccatgcctt gcggctcgac
     8461 taccgcctga tggaagaaat cggccagttt ttgcggcgtc agcagtttaa tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taagccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgatcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat
     9661 cagcgagaaa tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc
     9721 agctttttgc tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaaa taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac cggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag
    10261 ataggtaatc aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc agaactttac gcggcattgc cgggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac
    10501 cgccttcatc agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttatcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcaccgcatt acgctcacgt tcggcatatt tcttgtcgag
    10741 caaaggttca gcaatagcat cggccaggcg gtctaccgca ccaggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcaccacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac
    11161 taacaacaat aatgctttga accaggtgct gcggggcatt ctcggacctc ataatcaact
    11221 taattttctg tccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcggata gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagcct ttgataccag atatcatcac cttcgccacg caccatcatg ttaccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt
    11761 caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgagtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcgacgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gcccttccag aataaatggc tcggtgtcgg ggatttcgct gtcttcagta cggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 gatagttttg tggatcaaac ggcattcggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 gatagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgctga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagtaatgc cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga
    13381 taaacgcttc cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca cagcccgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg
    13561 ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga
    13861 catcctgttc accatcgctg ttaaagagct gcccggcatt ttcgctatca cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgggt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tgtagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttggcgcggt gccagcgcgg ttgcccgagt tgatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 taaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    14941 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    15061 tcttcaggga agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg
    15121 tggcgaaaaa acaatgtttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    15181 ataaccggca accaataagg cttcattatc tgccagtaaa cgcaaacaaa cccgggcatc
    15241 ggttccagcg tactgcgagc actgaactgc gggctgcgtc tgccaggagt gcattttccc
    15301 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ctatgctcac
    15361 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    15421 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    15481 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    15541 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    15601 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    15661 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcagactct
    15721 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    15781 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    15841 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc
    15901 ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    15961 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    16021 tttacaggca tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa
    16081 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    16141 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    16201 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    16261 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    16321 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc
    16381 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    16441 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    16501 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    16561 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    16621 ggagggtaaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    16681 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    16741 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    16801 ggttcgcggc ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtatcg
    16861 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    16921 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    16981 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    17041 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgggaa
    17101 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct
    17161 ggcgttggcc aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg
    17221 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    17281 tgcagaaacc acagcagttc gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    17341 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    17401 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    17461 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    17521 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    17581 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    17641 aacgcgccgt agccaatcag gaacaagcca gagacagctc ccattgggcg cggtttacga
    17701 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt
    17761 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    17821 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    17881 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga
    17941 ccaaacggaa tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg
    18001 cgggcaaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    18061 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    18121 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    18181 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac
    18241 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    18301 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    18361 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    18421 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    18481 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    18541 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    18601 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    18661 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    18721 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    18781 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    18841 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    18901 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    18961 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    19021 cgtccggcac gttcaatcag gcggcgtgct tcatcaactt catcgaggct tgtgaccatc
    19081 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    19141 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    19201 ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    19261 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    19321 tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    19381 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    19441 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    19501 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    19561 ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc
    19621 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    19681 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    19741 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    19801 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    19861 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    19921 tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat
    19981 tcgcgacgca gccgggtgtc cgaaagtagg tgcgagtaaa gatcgaaaat agccgccgtt
    20041 tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    20101 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    20161 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt
    20221 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc
    20281 gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    20341 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    20401 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    20461 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    20521 aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc
    20581 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    20641 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    20701 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    20761 acttaacctc ttttacgtcg ataagcagat gtgttttgtg gttttggcgt attttcctgc
    20821 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    20881 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    20941 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    21001 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    21061 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    21121 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    21181 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    21241 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    21301 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaagaaga cggtaaacca
    21361 ctctctgcag ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt
    21421 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    21481 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    21541 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta
    21601 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    21661 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    21721 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    21781 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt
    21841 gcagtgttgc gcaactcgat taccggcagc ctaaaaagca atataaatca gagaattgaa
    21901 caacacatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    21961 aaccaaggta tcatgacatg tcccaacctc gcccactcct ctctcctccc gaaactgaag
    22021 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    22081 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    22141 tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg
    22201 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    22261 tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc
    22321 acaagctcaa acgcgtactg tggataccgg ttgaaggtga gcgcagcatc ccgctggcgc
    22381 agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac
    22441 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggatcaccg
    22501 ctcgtcacgg ggagtattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
    22561 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
    22621 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    22681 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    22741 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    22801 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agctccccac
    22861 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    22921 gctgctggca tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag
    22981 tcaggaaatt tccgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa
    23041 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    23101 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    23161 ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg ccgccgtggt
    23221 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca
    23281 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtcggcttta ccctgattct
    23341 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    23401 tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac ccgctctgat actccgtacg
    23461 ctctcctgcg ggagggcgta ctcactcctg ttaccctcac agtttaagat tttcctgctt
    23521 tcaagatata taacgtcggt ttataaacag actattatca taggtgggcc aaatgaggat
    23581 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    23641 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    23701 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    23761 atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca
    23821 tccggctgta atgccgatca gttaag
//
LOCUS       DAJTXQ010000059        23377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000059 DAJTXQ010000000
VERSION     DAJTXQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.059"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="OO515_002215"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="OO515_002215"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6576141.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="OO515_002216"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="OO515_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6576142.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OO515_002217"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="OO515_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6576143.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OO515_002218"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="OO515_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6576144.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OO515_002219"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="OO515_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6576145.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="OO515_002220"
     CDS             5280..5738
                     /locus_tag="OO515_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6576146.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="OO515_002221"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="OO515_002221"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6576147.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="OO515_002222"
     tRNA            6790..6874
                     /locus_tag="OO515_002222"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OO515_002223"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OO515_002223"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO515_002224"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO515_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6576148.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO515_002225"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO515_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6576149.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO515_002226"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO515_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6576150.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OO515_002227"
     CDS             complement(10156..13899)
                     /locus_tag="OO515_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6576151.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO515_002228"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO515_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6576152.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OO515_002229"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OO515_002229"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6576153.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OO515_002230"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OO515_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6576154.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO515_002231"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO515_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6576155.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OO515_002232"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OO515_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6576156.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO515_002233"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO515_002233"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6576157.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO515_002234"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO515_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6576158.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OO515_002235"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OO515_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6576159.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..>23377
                     /locus_tag="OO515_002236"
     CDS             23294..>23377
                     /locus_tag="OO515_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6576160.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5859 a         6116 c         5627 g         5775 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtc
//
LOCUS       DAJTXQ010000060        23235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000060 DAJTXQ010000000
VERSION     DAJTXQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.060"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="OO515_002237"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="OO515_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6576161.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="OO515_002238"
     CDS             507..1136
                     /locus_tag="OO515_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6576162.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="OO515_002239"
     CDS             1163..2824
                     /locus_tag="OO515_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6576163.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="OO515_002240"
     CDS             3063..3122
                     /locus_tag="OO515_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6576164.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="OO515_002241"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="OO515_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6576165.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OO515_002242"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OO515_002242"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6576166.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OO515_002243"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OO515_002243"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6576167.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OO515_002244"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OO515_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6576168.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="OO515_002245"
     CDS             9505..10125
                     /locus_tag="OO515_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6576169.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="OO515_002246"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="OO515_002246"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6576170.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OO515_002247"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OO515_002247"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6576171.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OO515_002248"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OO515_002248"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6576172.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="OO515_002249"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="OO515_002249"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6576173.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OO515_002250"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OO515_002250"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="OO515_002251"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="OO515_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6576174.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="OO515_002252"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="OO515_002252"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6576175.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="OO515_002253"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="OO515_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6576176.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OO515_002254"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OO515_002254"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6576177.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="OO515_002255"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="OO515_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6576178.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="OO515_002256"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="OO515_002256"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6576179.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="OO515_002257"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="OO515_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6576180.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5653 a         6331 c         5906 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DAJTXQ010000061        23003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000061 DAJTXQ010000000
VERSION     DAJTXQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.061"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="OO515_002258"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="OO515_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6576181.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="OO515_002259"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="OO515_002259"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6576182.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="OO515_002260"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="OO515_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6576183.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="OO515_002261"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="OO515_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6576184.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="OO515_002262"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="OO515_002262"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6576185.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="OO515_002263"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="OO515_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6576186.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11312
                     /gene="ftsK"
                     /locus_tag="OO515_002264"
     CDS             7245..11312
                     /gene="ftsK"
                     /locus_tag="OO515_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6576187.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11467..12078
                     /gene="lolA"
                     /locus_tag="OO515_002265"
     CDS             11467..12078
                     /gene="lolA"
                     /locus_tag="OO515_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6576188.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12089..13432
                     /gene="rarA"
                     /locus_tag="OO515_002266"
                     /gene_synonym="mgsA"
     CDS             12089..13432
                     /gene="rarA"
                     /locus_tag="OO515_002266"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6576189.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13523..14815
                     /gene="serS"
                     /locus_tag="OO515_002267"
     CDS             13523..14815
                     /gene="serS"
                     /locus_tag="OO515_002267"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6576190.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15054..17498
                     /gene="dmsA"
                     /locus_tag="OO515_002268"
     CDS             15054..17498
                     /gene="dmsA"
                     /locus_tag="OO515_002268"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR6576191.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17509..18126
                     /gene="dmsB"
                     /locus_tag="OO515_002269"
     CDS             17509..18126
                     /gene="dmsB"
                     /locus_tag="OO515_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6576192.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18128..18991
                     /gene="dmsC"
                     /locus_tag="OO515_002270"
     CDS             18128..18991
                     /gene="dmsC"
                     /locus_tag="OO515_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6576193.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19027..19653)
                     /locus_tag="OO515_002271"
     CDS             complement(19027..19653)
                     /locus_tag="OO515_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6576194.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19968..21116
                     /locus_tag="OO515_002272"
     CDS             19968..21116
                     /locus_tag="OO515_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6576195.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21326..22756
                     /locus_tag="OO515_002273"
                     /pseudo
     CDS             21326..22756
                     /locus_tag="OO515_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(22757..>23003)
                     /locus_tag="OO515_002274"
     CDS             complement(22757..>23003)
                     /locus_tag="OO515_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6576196.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         5650 a         5726 c         6085 g         5542 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cataaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc atttttgtgg tgaaaacaag catcgtggca
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgactg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcatgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca accttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg gggcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccaga gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcgtcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgac
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcacccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 tgaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccgcc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt caggggcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccttg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc ggcacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
     8581 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
     8641 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
     8701 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccac
     8761 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
     8821 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
     8881 caccttctgt tgcagcagta cctccagtag aaaccgctgc cgctgtttct ccgctggcat
     8941 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
     9001 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
     9061 tgccacgacc gaaacgtatc cgcgtgccaa ctcgccgtga attggcgtct tacggtatta
     9121 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
     9181 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
     9241 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
     9301 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
     9361 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
     9421 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
     9481 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
     9541 agcagccaca acagccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
     9661 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcgc
     9721 cgcagcagca gtatcagcag ccacaacagc cagttgcgcc acaaccgcag gataccctgc
     9781 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
     9841 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
     9901 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
     9961 atgttgtcaa ttactctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    10021 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    10081 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    10141 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    10201 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    10261 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    10321 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    10381 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    10441 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    10501 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    10561 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    10621 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    10681 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    10741 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    10801 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    10861 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    10921 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    10981 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    11041 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    11101 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    11161 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    11221 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    11281 gtgaagtgct ggctccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    11341 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    11401 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    11461 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    11521 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    11581 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    11641 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    11701 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    11761 gcaacttggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    11821 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    11881 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    11941 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    12001 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    12061 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    12121 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    12181 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca
    12241 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    12301 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    12361 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    12421 tgttgacgaa gtgcaccgat tcaacaaaag ccagcaggat gcatttctgc cacatattga
    12481 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    12541 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgggtacag aggatattga
    12601 gcaagtacta actcaggcga tggaagacaa gacccgtggt tatggtggtc aggatattgt
    12661 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    12721 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
    12781 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
    12841 aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg gtagcgcacc
    12901 cgatgcggcg ctctactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    12961 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    13021 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    13081 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    13141 tgcgtttaaa gctgcgctgg cggatgctcg cgaacgcccg gattatgacg tgccggttca
    13201 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    13261 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    13321 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    13381 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    13441 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    13501 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    13561 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    13621 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    13681 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    13741 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    13801 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    13861 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    13921 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    13981 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    14041 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    14101 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    14161 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    14221 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    14281 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    14341 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    14401 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    14461 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    14521 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    14581 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    14641 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    14701 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    14761 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    14821 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    14881 caataattac tcttcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    14941 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    15001 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    15061 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    15121 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    15181 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    15241 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    15301 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    15361 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    15421 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    15481 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    15541 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    15601 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    15661 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    15721 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    15781 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    15841 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    15901 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    15961 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    16021 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    16081 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    16141 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    16201 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    16261 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    16321 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    16381 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    16441 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    16501 gtaactgcct ggttaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    16561 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    16621 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    16681 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    16741 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    16801 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    16861 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    16921 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    16981 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    17041 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    17101 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    17161 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg
    17221 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    17281 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    17341 caggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    17401 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    17461 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    17521 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    17581 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    17641 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    17701 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    17761 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    17821 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    17881 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    17941 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    18001 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    18061 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    18121 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    18181 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    18241 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    18301 ctttatcgct tctatgctcc atcttggttc accgatgcgc gcttttaact cgctcaaccg
    18361 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    18421 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctttg
    18481 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    18541 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    18601 gatgttcatg ggcggcccgt tactggggta cctgttgctg agtctggctg gcgtcgatag
    18661 ctgggcgatg cgtctgctgc cagccatttc cgtactggca ctggtggtga gtggcgtggt
    18721 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    18781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    18841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    18901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    18961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    19021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    19081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    19141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    19201 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaacacatc
    19261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    19321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    19381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    19441 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    19501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    19561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cagcagcatc
    19621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    19681 acgcctgaaa tcggtcagac gtgatgcatt gctctgaaag catagacggg aaatatgagt
    19741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    19801 aaaaacaatc agatgagatt aatcatctct tcagtattag cgttatgaat gagtaatata
    19861 aatcgataat accgccgttg cgatatgcgc gtattttcac ccattgacaa tgtttttggc
    19921 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
    19981 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
    20041 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
    20101 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
    20161 ttaaacgcat tggttttaac cgcagctatt atctggcctc cttcattttt gccgctggct
    20221 gtgccggcct tggcctgatg attggattct ggagttggtt ggcgtggcgt tttgtcgcgg
    20281 gcgtcggctg tgccatgatt tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt
    20341 cacgtaaccg tgggcgtttg cttgctgcat acatgatggt ttattacgtg ggaacgtttt
    20401 taggccagtt actggtcagc aaggtctcaa ccgagctgat gagcgtattg ccgtgggtta
    20461 caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
    20521 ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag gcgcggcttg
    20581 gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc ctgatgcctc
    20641 tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg gcggtactgg
    20701 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
    20761 tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg atgcttagcc
    20821 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
    20881 tggcgatggc atgggcttgc gagaaagttg aacatcatca actggtggcg atgaaccaag
    20941 ccttactgtt gagctatact gtgggaagtc tgcttggccc gtcatttacc gctatgctaa
    21001 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
    21061 tgctgatgct gctgcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
    21121 attcaagcag agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
    21181 ttgctttatt ccaaatttta ttcgtttaaa aataaaatgt gtagcaggtt ataattttgc
    21241 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
    21301 tataattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agctgcgatg
    21361 gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa
    21421 ctacgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg cgctctattt
    21481 cacaccttat gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg
    21541 ggtcagtacc tggattaaac acacgatggg acccggactg gcttatctcg ccgcgtggac
    21601 ctactgggtg gtgcatattc cctatctggc acaaaaaccc taggcaattc tgattgcgct
    21661 cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca
    21721 ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc
    21781 gctgaaaatc gtcggttctg tggcagggat tgctatgttt gttatgtcgc tcctgtatgt
    21841 ggcgatggcg gtaaccgcgc ctgcaattac tgaagtgcat attgcgacca caaacattac
    21901 ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt caatgctggt
    21961 tttcgcggtt ggcggagcag agaagatttc tccttacgtt aatcaaacgc gcaacccagg
    22021 aaaagaattt ccaaaaggga tgttatgcct ggcggtgatg gttgcggttt gtgccattct
    22081 gggctcgctg gcgatgggga tgatgtttga ttcgcgtaat atcccggatg acttaatgac
    22141 caacggtcag tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt
    22201 aatggtgatt tacgccattg cgaataccct gggacaagta gcggcgctgg tattctcgat
    22261 tgatgccccg cttaaagtgc tattaggtga tgctgacagc aaatatattc cagccagttt
    22321 atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtactggt
    22381 ggcgattctg attatgctgc cgactctcgg cattggtgat atgaacaatc tctataaatg
    22441 gctgttgaac cttaattcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt
    22501 tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta ttcgtaataa
    22561 gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctttg cctgtttgac
    22621 ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa
    22681 tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc tggcgcgtaa
    22741 agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt
    22801 ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt gggtaataga
    22861 tctggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa cgaccggtat
    22921 caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc atcaaaacgg
    22981 catccagttc agcatcaaca tca
//
LOCUS       DAJTXQ010000062        22593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000062 DAJTXQ010000000
VERSION     DAJTXQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.062"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            236..1342
                     /gene="ompC"
                     /locus_tag="OO515_002275"
     CDS             236..1342
                     /gene="ompC"
                     /locus_tag="OO515_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6576197.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1454..2509
                     /gene="apbE"
                     /locus_tag="OO515_002276"
     CDS             1454..2509
                     /gene="apbE"
                     /locus_tag="OO515_002276"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6576198.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2583..3647
                     /gene="ada"
                     /locus_tag="OO515_002277"
     CDS             2583..3647
                     /gene="ada"
                     /locus_tag="OO515_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6576199.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3647..4297
                     /gene="alkB"
                     /locus_tag="OO515_002278"
     CDS             3647..4297
                     /gene="alkB"
                     /locus_tag="OO515_002278"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6576200.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4373..6016
                     /gene="yojI"
                     /locus_tag="OO515_002279"
     CDS             4373..6016
                     /gene="yojI"
                     /locus_tag="OO515_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6576201.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6234..7880
                     /gene="mqo"
                     /locus_tag="OO515_002280"
     CDS             6234..7880
                     /gene="mqo"
                     /locus_tag="OO515_002280"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6576202.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8189..8677)
                     /gene="eco"
                     /locus_tag="OO515_002281"
     CDS             complement(8189..8677)
                     /gene="eco"
                     /locus_tag="OO515_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6576203.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            9085..9579
                     /gene="napF"
                     /locus_tag="OO515_002282"
     CDS             9085..9579
                     /gene="napF"
                     /locus_tag="OO515_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6576204.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9569..9832
                     /gene="napD"
                     /locus_tag="OO515_002283"
     CDS             9569..9832
                     /gene="napD"
                     /locus_tag="OO515_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6576205.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9829..12315
                     /gene="napA"
                     /locus_tag="OO515_002284"
     CDS             9829..12315
                     /gene="napA"
                     /locus_tag="OO515_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6576206.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12322..13017
                     /gene="napG"
                     /locus_tag="OO515_002285"
     CDS             12322..13017
                     /gene="napG"
                     /locus_tag="OO515_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6576207.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13004..13867
                     /gene="napH"
                     /locus_tag="OO515_002286"
     CDS             13004..13867
                     /gene="napH"
                     /locus_tag="OO515_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6576208.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13864..14313
                     /gene="napB"
                     /locus_tag="OO515_002287"
     CDS             13864..14313
                     /gene="napB"
                     /locus_tag="OO515_002287"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6576209.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14323..14925
                     /gene="napC"
                     /locus_tag="OO515_002288"
     CDS             14323..14925
                     /gene="napC"
                     /locus_tag="OO515_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6576210.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14944..15561
                     /gene="ccmA"
                     /locus_tag="OO515_002289"
     CDS             14944..15561
                     /gene="ccmA"
                     /locus_tag="OO515_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6576211.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15558..16220
                     /gene="ccmB"
                     /locus_tag="OO515_002290"
     CDS             15558..16220
                     /gene="ccmB"
                     /locus_tag="OO515_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6576212.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16262..16999
                     /gene="ccmC"
                     /locus_tag="OO515_002291"
     CDS             16262..16999
                     /gene="ccmC"
                     /locus_tag="OO515_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6576213.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16996..17205
                     /gene="ccmD"
                     /locus_tag="OO515_002292"
     CDS             16996..17205
                     /gene="ccmD"
                     /locus_tag="OO515_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6576214.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17202..17681
                     /gene="ccmE"
                     /locus_tag="OO515_002293"
     CDS             17202..17681
                     /gene="ccmE"
                     /locus_tag="OO515_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6576215.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17678..19621
                     /gene="ccmF"
                     /locus_tag="OO515_002294"
     CDS             17678..19621
                     /gene="ccmF"
                     /locus_tag="OO515_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6576216.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19618..20175
                     /gene="dsbE"
                     /locus_tag="OO515_002295"
     CDS             19618..20175
                     /gene="dsbE"
                     /locus_tag="OO515_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6576217.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20172..21224
                     /gene="ccmH"
                     /locus_tag="OO515_002296"
     CDS             20172..21224
                     /gene="ccmH"
                     /locus_tag="OO515_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6576218.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21259..21906)
                     /gene="narP"
                     /locus_tag="OO515_002297"
     CDS             complement(21259..21906)
                     /gene="narP"
                     /locus_tag="OO515_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6576219.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22226..>22593
                     /locus_tag="OO515_002298"
     CDS             22226..>22593
                     /locus_tag="OO515_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6576220.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5057 a         5668 c         6427 g         5441 t
ORIGIN      
        1 ttgatgttag gtgcttattt cgccattccg caataatctt aaaaagttcc cttgcattta
       61 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
      121 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
      181 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
      241 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
      301 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcctgca
      361 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
      421 aggcgtaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
      481 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
      541 gaaattccag gatgtgggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
      601 aacttcttgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
      661 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac actgacttct tcggtctggt
      721 tgacggcctg aactttgctg ttcagtacca gggtaaaaac ggtagcgtaa gcggcgaagg
      781 catgaccaac aacggtcgtg gtgctctgcg tcagaacggc gacggcgttg gcggatctat
      841 cacttatgat tacgaaggct tcggtatcgg tggtgcgatc tccagctcca aacgtactga
      901 tgatcagaac agcccgctgt acatcggtaa cggcgaccgt gctgaaacct acaccggtgg
      961 tctgaaatac gacgctaaca acatctacct ggctgctcag tacacccaga cctataacgc
     1021 aactcgcgta ggttccctgg gttgggcgaa caaagcacag aactttgaag ctgttgctca
     1081 gtaccagttc gacttcggtc tgcgtccgtc tgtagcatac ctgcagtcta aaggtaaaaa
     1141 cctgggtacc atcgctggtc gtaactacga cgacgaagat atcctgaaat atgttgatgt
     1201 tggtgctacc tactacttca acaaaaacat gtccacctac gttgactaca aaatcaacct
     1261 gctggacgac aaccagttca ctcgtgacgc tggcatcaac actgataaca tcgtagctct
     1321 gggtctggtt taccagttct aatctcgatt gatatcgaac aaagggcctg cgggcccttt
     1381 tttcattgtt ttcagcgtac aaactcattt ttttggtgta ctcttgcgac cgttcgcatg
     1441 aggataatca cgtatggaaa taagctttac ccgcgtggca ctgctggctg ccgcgctctt
     1501 ctttgttggt tgcgatcaaa aaccacaacc cgccaaaacc cacgctactg aagttaccgt
     1561 tctagaaggc aaaaccatgg gtaccttctg gcgtgccagc atcccgggca ttgatgccaa
     1621 acgcagtgcc gaacttaaag aaaagattca gacccagctg gacgccgacg atcagctgct
     1681 ttcgacctat aaaaaagatt ccgcgctgat gcgctttaac gattcgcaaa gtttatcgcc
     1741 gtggccggta agtgaagcga tggccgatat cgtcaccacc tcgctgcgca ttggcacgaa
     1801 gaccgatggc gcgatggata taaccgtcgg gccgctggtg aatctgtggg gctttggccc
     1861 ggaacaacag tcggttcaaa ttccgagcca ggaacagatc gatgcgatga aagccaaaac
     1921 cggcttacag cacctgacgg tcattaatca gtcgcatcag caatatctgc aaaaagacct
     1981 gccggattta aatgtcgatc tctccaccgt cggtgaaggt tatgcggcgg atcacctggc
     2041 acgcttgatg gagcaggaag ggatttcccg ctatctggtg tcggtgggcg gcgcgctgaa
     2101 cagccgtggt atgaacggtg aaggcctgcc gtggcgggta gcgattcaaa aaccaaccga
     2161 taaagaaaac gcggttcagg ctgtggtgga tatcaacggt catgggatta gcacctctgg
     2221 cagctaccgt aactattacg aactggacgg caaacgtctt tcccatgtta tcgatccgca
     2281 aaccgggcgt cccatcgaac acaatctggt atccgtgacg gtgattgctc cgacggcgct
     2341 ggaagccgat gcctgggata ctggcttgat ggtactcggg ccggagaaag ccaaagaggt
     2401 tgttcgccgg gaagggctgg cggtttatat gatcaccaaa gaaggcgata gctttaaaac
     2461 ctggatgtca ccacagttta aaagcttcct tatcagcgaa aaaaattaaa gcgcaagatt
     2521 gttggttttt gcgtgatggt gaccgggcag actaaaggct atccttaacc agggagctga
     2581 ttatgaaaaa cgccacatgc ttaactgacg atcaacgctg gcaatctgtc ttagcccgcg
     2641 acccgaatgc cgacggcgaa ttcgttttcg ccgtgcgcac cacgggtatc ttttgccgtc
     2701 cgtcttgccg cgccagacat gccttgcggg aaaatgtctc cttctacgca aatgccagcg
     2761 aggcactcgc cgccggattt cgcccctgca aacgttgtca gccagacaaa gccaatcccc
     2821 ggcaacatcg gttggataaa atcacccacg cgtgtcgact gctggaacag gaaacgcctg
     2881 taacgctgga agccttagcc gaccaggtgg cgatgagtcc atttcatcta catcggttgt
     2941 ttaaagcgac taccggaatg acgcctaaag cctggcaaca ggcctggcgc gctcgccgtt
     3001 tgcgcgaatc gctggcgaaa ggggagagcg tgacgacgtc tattcttaac gccggattcc
     3061 ccgacagcag cagttactac cgcaaagccg atgaaacgct gggcatgacg gctaaacaat
     3121 tccgtcacgg tggcgaaaat ctggcggtgc gttacgcgct ggctgattgt gagctgggtc
     3181 gttgcctggt ggcagaaagc gagcggggga tttgcgcgat attgctgggc gatgatgacg
     3241 cgacattaat cagcgagttg cagcagatgt ttcccgctgc cgacaacgcg cctgccgatc
     3301 tgacgtttca gcaacatgtg cgtgaagtga tcgccagcct caatcaacgc gatacgccgc
     3361 tgacgttacc gctggacatt cgcggcactg cttttcagca acaagtctgg caggcactgc
     3421 gcacgatacc ttgtggtgaa accgtcagtt atcagcaact ggctaacgcc atcggcaaac
     3481 cgaaagcggt acgggccgtt gccagcgcct gtgccgccaa caagctggct atcgtaatac
     3541 cctgtcatcg agtggtccgt ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc
     3601 gtaaagcgca actgctgcgc cgcgaagctg aaaatgagga gaggtaatgt tggatctgtt
     3661 tgccgatgct gaaccgtggc aagagccact ggcggctggt gcggtaattt tacggcgttt
     3721 tgcttttaac gctgcggagc aactgatccg cgatattaat gacgttgcca gccagtcgcc
     3781 gtttcgccag atggtcaccc ccgggggata taccatgtcg gtggcgatga ccaactgtgg
     3841 gcatctgggc tggacgaccc atcggcaagg ttatctctat tcgcccattg atccgcaaac
     3901 aaataaaccg tggcccgcca tgccacaaag ctttcatgat ttatgtcaac gtgcggctac
     3961 ggcggcgggc tatccagatt tccagccaga tgcttgtctt atcaaccgct acgctcctgg
     4021 cgcgaaactg tcgctgcatc aggataaaga cgaaccggat ctgcgcgcgc cgattgtttc
     4081 tgtttctctt ggcttacccg cgatttttca atttggtggc ctgaaacgta atgatccgct
     4141 caaacgtttg ttgttggaac atggcgatgt ggtggtatgg ggcggtgaat cgcggctgtt
     4201 ttatcacggc attcaaccgc tgaaagcggg gtttcatcca ctcaccggcg actgccgtta
     4261 caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg
     4321 cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact
     4381 tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg agctaagcct
     4441 cgccagtgcg gctttaggca ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac
     4501 ggcggatacc agtctgctgg tgttgccgga gtttctggga ttattgctgc tgttgatggc
     4561 aatcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact
     4621 gcgtagtgaa tttatcaagc ggattctcga tacccacgtc gagcgcattg aacaactcgg
     4681 tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt
     4741 gcgtctgccg gaactggtgc aggggatcat tctcactatc ggttcagcgg cgtatctgtg
     4801 gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcggtca ccatctgggg
     4861 cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctgcgtg aaaccgaaga
     4921 taagctgtac acggattttc aaaccgtact cgaagggcgc aaagagctga ctctgaaccg
     4981 tgaacgcgcc gagtatgtgt ttaacaacct ctacattcct gatgcgcaag agtatcgcca
     5041 tcatattatc cgcgcagaca ccttccatct tagtgccgtg aactggtcaa acatcatgat
     5101 gctgggcgct atcggcctgg tgttctggat ggcgaacagc ctcggttggg ctgataccaa
     5161 cgttgctgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc tttcggcggt
     5221 tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc
     5281 gctcgcgcct ttcaaagcag agtttccgcg cccgcaggcg tttcccaact ggcaaacgct
     5341 ggagctgcgt aatgtgacgt ttagctatca ggataacgcg ttttccgttg ggccgattaa
     5401 cctcaccatt aaacgtggcg agctgctgtt tctgattggt ggcaacggta gtggaaaatc
     5461 gacgctggcg atgttgctga cgggcttgta ccagccacaa agcggcgaaa tcttgctgga
     5521 tggcaaacct gtcagcggcg aacaaccgga agattatcgc aaactgtttt cggcagtgtt
     5581 taccgatgtc tggctgtttg atcaactgct ggggccggag gggcaacccg ctaacccgca
     5641 actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg
     5701 gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc
     5761 gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc aggatccaca
     5821 cttccgtcgt gagttttatc aggtgttgct gccgctgatg caggagatgg gtaaaactat
     5881 tttcgccatc agtcatgatg atcattactt tatccacgcc gaccgcctgc tggaaatgcg
     5941 caatgggcaa cttagcgagc tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt
     6001 tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgcc aggtcaatat
     6061 tctaaaaatc ccttcatttc taataaccct ataattaatc aacgaaatta taatgtttct
     6121 aaaattagaa tataatttat aaacattatt taaatgttgt tactttagtg ttaccgttga
     6181 tgccgcgcaa atctcacctg caataaagcg actaaaagta aggcattaac aagatgaaaa
     6241 aagtgactgc catgctcttc tcgatggccg tggggcttaa tgccgtttcg atggcggcaa
     6301 aagcgaaagc gtccgaggag caggaaactg atgtactgtt gattggcggc ggcattatga
     6361 gcgccacgtt ggggacctat ttacgcgagc tggagcctga atggtcgatg accatggtgg
     6421 agcgcctgga gggtgtcgcg caggagagtt cgaacggctg gaacaacgcc ggaaccgggc
     6481 attctgcact gatggaactg aactacaccc cgcaaaacgc cgatggcagt atcagtattg
     6541 aaaaagcagt cgccattaac gaagcatttc agatttcccg ccagttctgg gcgcaccagg
     6601 tcgagcgcgg cgtgctgcgt actccgcgtt catttatcaa taccgttccg catatgagct
     6661 ttgtctgggg cgaggataac gttaatttcc tgcgcgcccg ttacgccgcg ttgcaacaaa
     6721 gctcgctgtt tcgcggtatg cgttactctg aagatcacgc gcagatcaaa gagtgggcac
     6781 cgttagtgat ggaagggcgc gatccgcaac agaaagtggc agccacgcgt acggaaattg
     6841 gtaccgatgt gaactacggc gagatcaccc gccagttaat tgcttccttg cagaagaaat
     6901 ctaacttctc gctgcaactc agcagcgaag tccgcgccct aaagcgtaat gacgataaca
     6961 cctggaccgt taccgttgcc gatctgaaaa atggcactgc acagaacatt cgtgccaaat
     7021 ttgtctttat cggcgcgggc ggtgcggcgc tgaagctgtt acaggaatcg gggattccgg
     7081 aagcgaaaga ctacgccggt ttcccggtgg gcggacagtt ccttgtttcg gaaaacccgg
     7141 acgtggttaa tcaccatctg gcgaaggttt acggtaaagc atccgttggc gcaccaccga
     7201 tgtcggttcc gcatatcgat acccgcgttc tggacggtaa acgcgtagtg ctgtttgggc
     7261 catttgccac cttctcaacc aaattcctca aaaacggttc attgtgggat ctgatgagtt
     7321 ccaccaccac ctctaacgtg atgccgatga tgcacgtcgg gctggataat ttcgatctgg
     7381 tgaaatactt ggtgagtcag gtgatgttga gtgaagagga tcgttttgaa gcgttgaaag
     7441 agtactatcc gcaggcgaaa aaagaggact ggcgtttgtg gcaagcgggg cagcgtgtgc
     7501 agattatcaa gcgtgatgcc gataaaggtg gtgtactgcg tctgggtact gaagtggtca
     7561 gtgaccagca agggaccatt gccgcgctgc tgggggcatc gccaggggcg tcaaccgctg
     7621 cgccgattat gttggatctg ctggaaaaag tatttggcga tcgcgtttcc agcccgcaat
     7681 ggcaggctac gttgaaagcg atcgttccat cttatggacg caagctgaac ggtgatgtag
     7741 cggcaacaga acgcgagttg cagtacacca gcgaagtgct gggcctgaaa tacgacaagc
     7801 cgcaggcagc agatagtacg ccgaaaccgc agttgaaacc gcaacccgtt caaaaagaag
     7861 tggcggatat tgcgttgtaa tgatgtgcca catccggcat ggtatgccgg atgtggcgta
     7921 accgccttat ccgacctacg gggtggagca cttcacagcg gtgtaggcct gataagacgc
     7981 gtcagcgtcg catcaggcaa ccggctcggg cgttagatgt cggatgcggc gtaaccgcct
     8041 tatccaacct acggggtgga gcacttcaca gcggtgtagg cctgataaga cgcgccagcg
     8101 tcgcatcagg caaccggctt gggcgttaga tgtcggatgc ggcgtgaccg ccttatccga
     8161 cctacggggt gacgcacttc acagcagttt agcgaactac cgcgttgtca attttctctt
     8221 ccgccttcca gacgcggtac ttcacatcta cattgtctgg cgtatacacc acgatcggca
     8281 gcttgctgtt gtaacgcagc attccagcat cgcccagata cgcggtgaca aatttcttct
     8341 ctttcttgcc atccgggcag gccatcatcg tggaaaccgg cgagctaact ttatcaaaga
     8401 cataataatc atagccccag ccttccagcg ttttgttttc cagcttcccg ccgagacgat
     8461 gcaaattgca atcgacttcc agcgtctgac cggttaacag ttctactttc agggtagatt
     8521 catcttcttg cggggttaac tgaatcacct gacgcttcat ccctttttca gcttgtggat
     8581 aaggcgcgat tttttccagt ggctggacgc tttctgccgc ccaggcggaa gtggtggcga
     8641 aagcggcaaa caatactgca ggtagaatgg tcttcatttt tttgctttcc ttcttcattt
     8701 ataggttttc accgatgatg tcgctgaagg ttaacatatt catcagattt attaatttgc
     8761 tttatgaata acttaaagaa cattcagaca attttacctg ctcttttagt gttaaattct
     8821 gatgagggag caaaccagca gaaatatctc tggtgaatta atcgctatat aaatatgttt
     8881 ataaccattt gaaatgtgag caaaagcccg tttttgccac gcttcccgcc actcttttga
     8941 tcctgctaca ggttttaccc cgatcggggt atgcatcttt gacacatcct ttaatatctt
     9001 agcggctata aaaatggctt attaattatg cggtttattt ggtcgctctc aattttcaga
     9061 gcgcgttaat gatggaaggt caatgtgaag attgatgcat cccgtcgggg catactcact
     9121 ggtcgctggc gcaaagccag taacggtatc cgtccgccct ggtccggtga tgaatctcat
     9181 tttctgaccc attgcacccg ttgcgacgcc tgtattaatg cttgtgaaaa caacattctg
     9241 caacgcggcg cgggcggcta tccgagcgtt aatttcaaaa ataacgagtg ctgcttctgt
     9301 tacgcctgcg cgcaggcttg ccccgaatcg ctattttctc cgcgccacac cagggcctgg
     9361 gatttgcagt tcaccattgg ggacgcgtgc ctggcgtatc agtcagttga atgccgccgc
     9421 tgtcaggata gctgtgagcc aatggcaatt atctttcgcc cgacgctctc cgggatctac
     9481 cagccgcaac ttaatagcca actttgcaac ggatgtggag catgtgcagc cagctgtccg
     9541 gtatcagcca taactgcgga gtatctccat gcacactaac tggcaagttt gcagcctggt
     9601 cgtgcaggcc aaaagcgaac gaatttcaga catcagcacc caactgaacg cctttcccgg
     9661 ctgtgaagtt gctgtcagcg acgcgccgag cggtcagttg attgtggtgg tggaagcaga
     9721 agacagcgaa acgctgatcc aaaccattga gtcagtacgc aacgtagagg gcgtgctggc
     9781 ggtgtcgctg gtttatcacc agcaggaaga gcaaggtgag gaaacaccat gaaactcagt
     9841 cgtcgtagct ttatgaaagc taacgccgtt gcggccgctg cggcggctgc cggtctcagc
     9901 gtgccgggcg ttgcccgcgc cgttgttggt cagcaggaag ccattaaatg ggataaagcg
     9961 ccgtgccgtt tctgcggtac tggttgcggc gttctggtcg gaacgcagca ggggcgtgtg
    10021 gtggcctgtc agggcgaccc ggacgcaccg gttaaccgtg gcctgaactg cattaagggc
    10081 tatttcctgc ccaagatcat gtacggtaaa gaccgtttga cgcagccgct gctgcgtatg
    10141 aaaaacggta aatatgacaa agaaggcgaa tttaccccaa tcacctggga tcaggccttc
    10201 gatgtgatgg aagagaaatt caaaaccgcc ctgaaagaaa aagggccgga atcgatcggt
    10261 atgttcggtt ctggtcagtg gactatctgg gaaggttatg ccgcgtccaa gctgtttaaa
    10321 gcgggcttcc gttcgaacaa catcgacccg aacgcgcgtc actgtatggc gtcggcagta
    10381 gttggcttta tgcgtacctt tggtatggat gagccgatgg gctgctatga cgacatcgag
    10441 caggctgacg cgtttgtgct gtggggcgca aacatggcgg agatgcaccc gatcctctgg
    10501 tcgcgcatca ctaaccgtcg tctctctaat cagaacgtca ccgtggcggt gctttctacc
    10561 taccagcatc gtagcttcga gctggcggat aacggcatca tctttacgcc acagtctgac
    10621 ctggtgatcc tgaactacat cgccaactat atcattcaaa acaatgcgat aaatcaggac
    10681 ttcttcagca aacacgttaa cctgcgcaaa ggggcgacgg acatcggcta cggtttacgt
    10741 ccgacccatc cgctggaaaa agcagcgaag aatccaggtt ctgacgcctc cgaaccgatg
    10801 agctttgaag attacaaagc cttcgttgcc gagtatacgc tggaaaaaac cgctgaaatg
    10861 accggcgtgc cgaaagacca gttagaacaa ctggcgcagc tgtatgccga tccgaacaag
    10921 aaagtcatct cctactggac gatgggcttc aaccagcata ctcgtggcgt gtgggctaac
    10981 aacctggtct acaacctgca cctgctgacc ggcaaaattt cccagccggg ttgcggtccg
    11041 ttctccctga ccgggcaacc ttctgcgtgt ggtactgcgc gtgaagtggg cacctttgct
    11101 caccgtctgc ctgcggacat ggtggtgacc aacgagaaac accgtgatat ctgcgagaag
    11161 aagtggaata tcccgagcgg caccattccg gcgaaaatcg gtctgcacgc ggtggcacaa
    11221 gaccgtgcgc tgaaagacgg caaactgaat gtttactgga ccatgtgtac caacaacatg
    11281 caggccgggc cgaacatcaa tgaagagcgt atgccgggct ggcgcgatcc gcgcaacttc
    11341 atcatcgtct ccgatccgta tccgacagtc agtgcgctgg ccgccgactt gatcctgccg
    11401 accgcaatgt gggtagagaa agagggtgct tacggtaacg ccgaacgccg tactcagttc
    11461 tggcgtcagc aggtacaggc accgggcgaa gcgaaatcgg atctctggca gttagtccag
    11521 ttctcccgcc gcttcaaaac tgaagaagtg tggccggaag agctgctagc gaagaaaccg
    11581 gaactgcgtg gcaaaacgct gtacgaagtg ctttatgcca caccggaagt gagcaaattc
    11641 ccggtatccg aactggcgga agatcagctg aacgatgaat cccgcgagct gggcttctat
    11701 ctgcaaaaag ggctgttcga agagtacgca tggtttggtc gcggtcacgg tcacgatctc
    11761 gcgccgttcg atgactacca caaagcgcgc ggtctgcgct ggccggtggt gaacggtaaa
    11821 gaaacgcagt ggcgttacag cgaaggtaac gacccgtacg tgaaagcggg cgaaggctat
    11881 aagttctacg gtaaaccgga tggtaaagcg gtgatcttcg cgctgccgtt cgaaccggcg
    11941 gcggaagcac cggatgaaga gtacgacctg tggctctcta ccggacgcgt tctggagcac
    12001 tggcacactg gcagtatgac tcgccgtgtg ccggaactgc accgcgcctt cccggaagcg
    12061 gtcttgttta ttcacccgct ggatgcgaaa gcgcgcgatc tgcgccgtgg cgacaaagtg
    12121 aaagtggttt ctcgccgtgg cgaagtgatc tcgattgttg aaacgcgcgg tcgtaaccgt
    12181 ccgccacagg gcctggtgta catgccgttc ttcgacgccg cacagctggt taacaaacta
    12241 acgctggacg cgaccgatcc gctctcgaaa gagacggact tcaagaagtg cgcagtcaaa
    12301 ctggagaagg tgtaagccat tatgtcccgg tcagcgaaac ctcaaaatgg tcgccgccgc
    12361 tttctgcgcg atgttgttcg cacagcaggc gggctggctg ccgtgggtgt ggcgctgggg
    12421 ttacaacagc aaaccgcacg cgcatctggc gtgcggttgc gcccgcccgg agccataaac
    12481 gagaacgcct ttgccagtgc ctgtgtgcgt tgcggtcagt gtgttcaggc ttgcccttac
    12541 gacaccttaa aactggcgac gctggcttct ggcctgtcgg cgggcacgcc atattttgtc
    12601 gcacgggata ttccttgcga aatgtgtgag gacattccgt gcgccaaagt gtgcccgagt
    12661 ggtgcgctgg atcgtgagat cgaatcgatc gacgacgcgc ggatggggct ggcggtgctg
    12721 gtggaccagg aaaactgtct caactttcag gggctgcgct gcgatgtttg ttatcgcgaa
    12781 tgcccgaaaa ttgatgaggc catcaccctg gagctggagc gcaacacgcg taccggtaag
    12841 cacgcccgct ttctgccgac ggttcacagc gacgcctgta ccggctgcgg taagtgcgaa
    12901 aaagtgtgcg tgctggaaca accggcaatc aaggtgttac cgctgtcact ggcgaaaggg
    12961 gagttaggtc accattaccg cttcggctgg ctggagggga acaatggcaa atcgtaaacg
    13021 tgacgccggg cgcgaggcgc tggtgaaaaa aggctggtgg cgcagtcacc gttggctggt
    13081 gttacgtcgc ctttgtcagt tcttcgtgct ggggatgttt ttgagcggtc cgtggtttgg
    13141 tgtgtggatc ttacacggca actacagctg tagcctgtta ttcgacaccg ttccgctgac
    13201 cgatccgctg atgacgctgc aaagtcttgc cagtggtcat ctgcctgcca cggtggcgtt
    13261 gaccggtgcg gtgataatca ctgtgctcta cgccctggca ggaaagcgat tattttgcag
    13321 ctgggtctgc ccgctgaacc cgattaccga cttagcgaac tggttacgca ggcgctttga
    13381 cctcaatcag tctgcgacga tccctcgcca cattcgctac gtgctgttgg tggtcattct
    13441 gctgggatca gccttaaccg gcacgctcat ctgggaatgg atcaaccccg tttccctgat
    13501 ggggcgtagc ctggtaatgg gcttcggcag cggcgcgctg cttattctcg cgctgttttt
    13561 atttgatcta ctggtcgttg aacacggctg gtgcgggcac atttgtccgg ttggcgcgct
    13621 gtatggcgtg ctgggtagca aaggtgtgat taccgttgcg gccagcgatc ggcagaaatg
    13681 taaccgctgt atggattgtt ttcatgtttg cccggaaccg catgtgctac gtgccccggt
    13741 gctggatgaa caaagcccgg tgcaggtcac cagccgcgat tgcatgacgt gcggtcgctg
    13801 cgtggatgtc tgttctgagg atgtatttac aataactaca cgatggagtt cgggagcgaa
    13861 atcatgaaaa gccatgacct gaagaaagcg ctgtgtcaat ggacggcgat gctggccctg
    13921 gtggtaagcg gcgcggtttg ggcggctaat ggcgtcgatt ttagccagtc gccggaagta
    13981 tccgggacgc aggaaggggc cattcggatg ccaaaagagc aggatcggat gccgctgaac
    14041 tatgtgaatc agccgccaat gatcccgcat agcgttgaag gttatcaggt aacgaccaat
    14101 accaaccgct gcttgcagtg ccacggtgtc gaaagctatc gcaccactgg cgcgccgcgt
    14161 atcagtccta ctcactttat ggacagcgac ggcaaagtgg gcgcggaagt ggctccgcgt
    14221 cgttatttct gtctgcaatg tcacgtaccg caggccgata ccgcgccaat cgtggggaat
    14281 acctttaccc catcaaaagg ttacgggaaa taagaggtta ttatgggaaa ttctgaccgt
    14341 aagcctggtc tgattaagcg cctgtggaaa tggtggcgta cccccagccg tctggcgctg
    14401 ggaacgctgc tgttgatcgg ttttgtcggc ggcatcgtct tctggggcgg ctttaacacc
    14461 ggaatggaaa aagccaatac cgaagagttc tgcattagct gccacgaaat gcgtaacacg
    14521 gtgtatcagg aatacatgga ttccgtgcac tacaacaacc gtagcggcgt ccgtgcgacc
    14581 tgtccggatt gtcacgttcc gcacgagttt gtgccgaaga tgatacgcaa gctcaaagca
    14641 agtaaagagc tgtatggtaa aatttttggc gttattgaca cgccgcagaa atttgaagct
    14701 catcgtctga caatggcaca gaatgagtgg cggcgcatga aggacaataa ctcgcaggag
    14761 tgccgtaact gtcacaactt cgagtatatg gatacaaccg cccagaaatc ggttgccgcg
    14821 aagatgcatg accaggcggt gaaagatggg caaacctgta ttgattgcca taaagggata
    14881 gcgcacaagc tgcccgatat gcgtgaagtc gagccaggtt tttaacaggg ttattgcgtg
    14941 ggtatgcttg aagccagaga gttactttgt gagcgggatg aacgaacctt atttagtggc
    15001 ttgtcattta cgctgaacgc aggagagtgg gtacaaatca ccggtagcaa cggcgcgggg
    15061 aagacaacgc ttctccgttt gctgacgggg ttgtctcgcc ctgacgcagg cgaggttctc
    15121 tggcaagggc agcccttgca tcaggtacgc gacagctacc atcaaaacct gttatggata
    15181 ggccatcagc cggggatcaa aacccggctg acggcgttag aaaatctgca cttttatcat
    15241 cgcgatggcg ataccgcaca atgtctggaa gccctggcgc aggccgggct tgccggattc
    15301 gacgatattc cggtaaatca actttctgcc gggcaacaac gccgcgtcgc tttagcgcgt
    15361 ctgtggctga cccgtgccac gttatggatc ctcgacgagc cttttaccgc gattgacgtt
    15421 aacggcgtcg atcgtctgac ccagcgtatg gcgcagcata cggagcaggg ggggattgtg
    15481 attctgacta cccaccagcc gctcaacgtt gctgaaagta aaattcgccg catttcactg
    15541 acgcaaacga gggccgcatg atgttctggc gcattttccg tcttgagctg cgtgtagcgt
    15601 ttcgccatag cgccgaaatc gccaacccgc tgtggttctt cctgattgta attacccttt
    15661 ttccgctcag tatcggtccg gagccgcaac tgctggcgcg tattgcaccg ggcattatct
    15721 gggttgctgc gctgctttca tccttgctgg cgctggaacg actgttccgt gacgatttgc
    15781 aggacggcag tcttgaacaa ttgatgttgt tgccgttacc cttgcccgcc gttgtgctgg
    15841 cgaaggtgat ggcgcactgg atggtaaccg gtctgccgtt actcatcctt tcgccactgg
    15901 tagcaatgct actgggaatg gatgtttatg gctggcaagt gatggcgctg acgctgctgc
    15961 tgggaacgcc tacgcttggc tttctcggtg caccgggtgt ggcgttgaca gtgggactta
    16021 agcgcggtgg tgtgctgctc agcatactgg tgttaccgct gactatccca ttactcatct
    16081 ttgccaccgc cgcgatggac gcggcttcca tgcatttgcc cgttgacggg tatctggcaa
    16141 ttttaggcgc gttgctggca ggcaccgcga cattaagtcc ttttgcgacg gcggcagcgt
    16201 tacgaatcag cattcaataa cgctggatta ctgatttttg ttcgagtctg gtatcgaaac
    16261 tatgtggaaa acactgcatc aactggcgat cccaccacgg ctgtatcaaa tctgtggctg
    16321 gtttataccg tggctggcaa ttgccagtgt ggtcgtgctt accgtcggct ggatctgggg
    16381 attcggcttt gctccggctg attatcagca gggaaatagc taccgcatta tctacctgca
    16441 tgtgcctgcg gcgatctggt cgatgggcat ttatgcatca atggcagtgg cagcgtttat
    16501 tggccttgtc tggcagatga aaatggccaa cctggcggtg gcggcgatgg cccccattgg
    16561 tgccgtgttt acctttattg ccctggttac cggctctgca tggggaaaac cgatgtgggg
    16621 cacctggtgg gtatgggatg cacgtctgac ttctgaactg gtgctgctgt ttttgtatgt
    16681 gggtgtgatt gccctgtggc acgccttcga cgaccgccgt ctggcgggcc gtgcggcagg
    16741 tatcctggtg ctgattggcg tggtgaatct gccgattatt cattactccg tggagtggtg
    16801 gaacaccctg catcagggat caacgcggat gcagcaaagt atcgatccgg cgatgcgttc
    16861 gccgctgcgc tggtcgattt ttggcttcct gctcctgtct gccacgctga cgctgatgcg
    16921 gatgcgtaat ttgattttgc tgatggaaaa acgccgtccg tgggtgagtg aactgatact
    16981 gaaaagaggc cgtaaatgac ccctgcattt gcttcctgga atgaattttt cgcaatgggc
    17041 ggttacgcct tttttgtctg gctggcggtg gtgatgaccg ttattccgct ggtggttttg
    17101 gtcgtgcact cggtgatgca acatcgcgca attctgcgtg gtgtggcgca acagcgggcg
    17161 cgtgaggcgc gtttacgtgc tgcgcaacag caggaggctg catgaatatt cgccgtaaaa
    17221 accgcttgtg gattgcctgt gccgtgttgg cagggctggc gctgactatc ggtctggtgc
    17281 tatatgcgct gcgctcgaat atcgatctct tttatacgcc gggggaaatt ctctacggca
    17341 agcgtgaaac tcagcaaatg ccggaagtcg gtcagcgttt gcgcgttggc gggatggtga
    17401 tgctgggtag tgtgcagcgc gatcccaatt cgctgaaagt gaccttcact atttacgatg
    17461 ctgaaggctc agtggatgtc tcttacgaag gcattttgcc ggatctgttc cgtgaagggc
    17521 agggcgttgt ggtgcagggc gagctggaaa aaggcaatca tatcctcgcg aaagaagtgc
    17581 tggcgaaaca cgacgaaaac tacacgccgc cagaagttga gaaagcgatg gaagctaacc
    17641 accgtcgccc ggcgagtgtt tataaggacc cagcatcatg atgccggaaa ttggaaacgg
    17701 gctgctgtgt ctggcgttag ggattgcgct gctgctgtcc gtgtatccgc tatggggcgt
    17761 ggcgcgcgga gatgcgcgca tgatcgcgtc ttcccgcttg tttgcctggc tgctgtttat
    17821 gtctgtggct ggcgcatttc tggtgctggt caatgctttc gtggtcaacg actttaccgt
    17881 cacctatgtt gccagctact ccaataccca gcttccggtg tggtatcgcg tggcggctac
    17941 ctggggcgcg catgaaggct cgctactgct gtgggtgctg ctgatgagcg gctggacctt
    18001 tgcggtggcg atttttagtc agcgtattcc gctggatatt gtggcccgcg tactggcgat
    18061 aatggggatg gtcagcgtcg gctttttgct gttcattctc tttacctcta acccattctc
    18121 acgcacgctg ccgaacttcc cgattgaagg gcgcgatctt aacccgctgt tacaggaccc
    18181 ggggctgatc ttccatccgc ctctgcttta tatggggtac gtgggtttct cggtggcgtt
    18241 tgcttttgcc attgcttctt tgctgagcgg gcgtctggac agcacttatg cgcgttttac
    18301 tcgcccgtgg acgctggcgg cgtggatttt cctgacgctc ggcattgtgc tcggttccgc
    18361 atgggcctat tacgaactcg gctggggggg ctggtggttc tgggatccgg tagaaaacgc
    18421 ctcgtttatg ccgtggctgg tggggactgc gctgatgcac tcactggcgg tcactgaaca
    18481 acgcgccagc ttcaaagcgt ggacattact gctggcaatc agtgccttct cgttgtgtct
    18541 gttggggact ttcctcgtgc gttccggcgt gctggtatcg gtacacgcgt ttgcgtctga
    18601 tccggcgcgc ggtatgttta tcctcgcctt tatggtgctg gtgattggag gttcgctgct
    18661 gctgtttgcc gcgcgtggac acaaagttcg ctcacgcgta aacaatgcgc tgtggtcgcg
    18721 ggaatctctg ctgttagcga acaacgtttt gctggtcgcc gcgatgctgg tggtgttgct
    18781 ggggacgctg ctgccgctgg tgcacaagca actgggactg ggcagtattt cgattggcga
    18841 accgttcttc aacaccatgt ttacctggct gatggtgccg tttgcgctac tgctgggtgt
    18901 cggtcctctg gtgcgctggg ggcgcgatcg cccacgtaaa atccgcaatt tattgattat
    18961 cgccttcatc tcaacgctgg tgctgtctct gctgttgccg tggctgtttg agagcaaagt
    19021 tgtggcgatg acggtgctcg gcctggcaat ggcctgctgg attgcggtgc tggcaattgc
    19081 ggaagctgcg ctgcgtattt cacgcggcac gaaaaccacc ttcagttatt gggggatggt
    19141 ggctgctcac ctggggctgg cagtgacaat tgttggcatt gcttttagcc agaactatag
    19201 cgttgagcgt gatgtgcgca tgaagtccgg cgatagcatc gatattcatg aatatcgctt
    19261 caccttccgt gatgtcaaag aggtgactgg cccgaactgg cgtggcggtg tggcgactat
    19321 cggcgtaacg cgagagggta aaccggaaac agtgctgtat gcggaaaaac gttattacaa
    19381 cactgccggg tcgatgatga ccgaagcggc gattgacggt ggcatcacgc gtgacctgta
    19441 cgccgcgctc ggtgaagagc tggaaaacgg cgcgtgggct gtgcgtcttt actacaaacc
    19501 atttgttcgc tggatttggg cgggcgggct gatgatggcg ttgggcggac tgctgtgtct
    19561 gtttgatcct cgctatcgta agcgcgtgag tccgcaaaaa actgcgccgg aggctgtatg
    19621 aagcgcaaag tattgttaat tccgttgatt atcttcctgg cgattgccgc ggcgctgctg
    19681 tggcagctgg cgcgtaatgc cgaaggggat gatccgacca atctggaatc ggcgctcatt
    19741 ggcaagccag tgccgaagtt tcgcctcgaa tcactggaca atccggggca gttttatcag
    19801 gcggatgtgc tgactcaggg caaaccagtg ctgcttaacg tctgggcgac ctggtgtccg
    19861 acctgccgtg cggaacatca atatctgaat cagctttctg cgcagggcat ccgcgtagtc
    19921 ggcatgaact ataaagacga tcgccagaag gcaatcagct ggctgaaaga gctgggcaat
    19981 ccttacgcgc taagcctgtt tgatggcgat ggcatgttag ggctggatct cggtgtctat
    20041 ggcgcgccag aaacgttcct tattgacggc aacggcatca ttcgctatcg ccatgcgggc
    20101 gatctcaatc ctcgcgtctg ggaagaagag atcaagccgc tgtgggagaa atacagtaag
    20161 gaggccgcac aatgaggttt ttattgggca tgctgatgct gatgatctcc ggctcagcgc
    20221 tggcgaccat agacgtgttg cagtttaaag atgaagcaca ggaacaacag ttccgtcagc
    20281 tcactgaaga actgcgctgc ccgaaatgcc agaacaacag cattgccgat tccaactcga
    20341 tgattgccac cgacctgcgc cagaaagtgt atgaactgat gcaggaaggt aaaagtaaaa
    20401 aagagattgt cgattatatg gtggcgcgtt acggcaactt cgtcacttac gatccgccgt
    20461 taacgccgct gaccgtgctg ctgtgggtgc tgccagtagt agctattggc attggcggtt
    20521 gggtcatata cgcccgctcg cggcgtcggg tacgcgtggt gccggaagcg tttcctgaac
    20581 aaagcgtgcc ggaaggtaag cgtgccggat atgtcgctta tttgccgggc attgtggtgg
    20641 cgttaattgt ggctggcgtc agctactacc agaccggaaa ttatcaacag gtgaaaatct
    20701 ggcagcaggc cacggcacag gctccggcgt tgctggacag ggcgctggat ccgaaagctg
    20761 atccgctcaa cgaagaagag atgtcgcgcc ttgcgctggg gatgcgtact caactgcaaa
    20821 aaaatccggg agatatagac ggctggatta tgttgggccg cgttggcatg gcgctgggta
    20881 acgccagtat cgccaccgat gcatacgcta ctgcgtatcg cctcgatccg aaaaacagtg
    20941 atgccgcact gggttatgct gaagcgttga cacgttcatc tgatcccaac gacaaccgcc
    21001 tcggcggcga actgctacgt cagctggtga gaacggacca tagcaatatc cgtgtgctaa
    21061 gcatgtatgc gtttaatgcc tttgagcagc agcgatttgg cgaagccgtt gccgcgtggg
    21121 agatgatgtt gaaactctta cctgccaacg atactcgccg tgcggtgatt gaacgtagta
    21181 tcgcgcaggc gatgcaacat ttgtcgccgc aggagagtaa ataaaaagag gcccgatggt
    21241 gatgccatcg ggctattttt attgtgcccc gcgttgttgc aggaacagaa tggtggccgc
    21301 cacgcgtgag cggacattga gtttacgcag cagatttcga atatgaactt ttactgtctg
    21361 ctcggaaata ttcaacacag aggcaatctg tttatttgac agcccctgtg ccagctcgtg
    21421 cagaacatcc agctcgcgct ccgtcagcac gctgaaggga tcttcttccg cgccaaacat
    21481 ttcacgttca cgtaagtact gattgacgcg ttcgctaaag actttgctgc ctttcgctcc
    21541 ggcacgaatc gcttccagca acacttccgg gtcgctgtct ttcagcagat aaccgtctgc
    21601 gcctgcgtct atcagcgcaa agacatcgct ggaggcatcg gatacggtca ggataataat
    21661 ttgcgcggta acgccatccc tgcgcaaggc attgagggta tccaggccac tcataccttt
    21721 catattgaga tccagcaaga tcacgtcgat atccagtcta ttcgccagat cgatcgcgct
    21781 cgcgccgtcg cccgcttcgg cgaccacttc aaagccagga tcaagctcca gtaactgacg
    21841 aacaccgcgt cgcataagtg gatgatcatc cacaatcatc acctgaaaag gcgttgcttc
    21901 aggcatagta gtctcctgag gttttattag aatcattatg tttgagcgta gcgttggtaa
    21961 cgctaccagg cgcgtgaatg ttaccccatt ttatgtatgg acatgtagtg aaaactacaa
    22021 ccttgccgtt cgctttggca tcattttcat tataaagacg gaattttgca tcagcttctc
    22081 atcaagaaaa acaactaaaa aatcaatcac aatacttgtg catttcttta aacagttttt
    22141 aaaaactgac tccgggtatg gagctatggg tattttctgt acccaatgct tttaacagca
    22201 attaatttca taggatgaaa gctcaatgca tcaatctggt tctgtttctc tttgtcgttc
    22261 cgcaatatct gttctggtgg ctacagcgtt atattcaccc atagcattgg catcaacagt
    22321 tgagtatggt gagacagttg atggtgttgg cctggaaaaa gatatccagc tggtttatgg
    22381 gaccgccaat aatacgaaaa tcaatcctgg cggagaacag catataaaag aatttggtgt
    22441 aagtagtaat actgaaatta acggcgggta tcagtacatt gaaatgaatg gcactgcaga
    22501 atactcagta ttaaatgatg gttatcaaat tgttcaaatg ggtggcgcgg taaaccagac
    22561 tacgctcaat aatggagtgc tacaggttta tgg
//
LOCUS       DAJTXQ010000063        22235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000063 DAJTXQ010000000
VERSION     DAJTXQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.063"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..1461
                     /locus_tag="OO515_002299"
     CDS             73..1461
                     /locus_tag="OO515_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6576221.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1504..2007
                     /gene="yeaK"
                     /locus_tag="OO515_002300"
     CDS             1504..2007
                     /gene="yeaK"
                     /locus_tag="OO515_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6576222.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2008..2112)
                     /locus_tag="OO515_002301"
     CDS             complement(2008..2112)
                     /locus_tag="OO515_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576223.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2282..2728
                     /locus_tag="OO515_002302"
     CDS             2282..2728
                     /locus_tag="OO515_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6576224.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="OO515_002303"
     CDS             complement(2685..3506)
                     /gene="nimR"
                     /locus_tag="OO515_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6576225.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3603..4784
                     /gene="nimT"
                     /locus_tag="OO515_002304"
     CDS             3603..4784
                     /gene="nimT"
                     /locus_tag="OO515_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6576226.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4839..5186
                     /locus_tag="OO515_002305"
     CDS             4839..5186
                     /locus_tag="OO515_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6576227.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="OO515_002306"
     CDS             complement(5208..5462)
                     /gene="yoaF"
                     /locus_tag="OO515_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6576228.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5645..6670
                     /gene="dgcP"
                     /locus_tag="OO515_002307"
     CDS             5645..6670
                     /gene="dgcP"
                     /locus_tag="OO515_002307"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6576229.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6703..6801
                     /locus_tag="OO515_002308"
     CDS             6703..6801
                     /locus_tag="OO515_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6576230.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6804..6878
                     /gene="yoaJ"
                     /locus_tag="OO515_002309"
     CDS             6804..6878
                     /gene="yoaJ"
                     /locus_tag="OO515_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6576231.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6937..7185)
                     /locus_tag="OO515_002310"
     CDS             complement(6937..7185)
                     /locus_tag="OO515_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6576232.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7333..7515)
                     /locus_tag="OO515_002311"
     CDS             complement(7333..7515)
                     /locus_tag="OO515_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR6576233.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="OO515_002312"
     CDS             complement(7519..7878)
                     /gene="yeaR"
                     /locus_tag="OO515_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR6576234.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="OO515_002313"
     CDS             complement(8051..8689)
                     /gene="leuE"
                     /locus_tag="OO515_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR6576235.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="OO515_002314"
     CDS             complement(8816..9739)
                     /gene="dmlR"
                     /locus_tag="OO515_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6576236.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9842..10927
                     /gene="dmlA"
                     /locus_tag="OO515_002315"
     CDS             9842..10927
                     /gene="dmlA"
                     /locus_tag="OO515_002315"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6576237.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11178..12788
                     /gene="yeaV"
                     /locus_tag="OO515_002316"
     CDS             11178..12788
                     /gene="yeaV"
                     /locus_tag="OO515_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6576238.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12820..13944
                     /gene="yeaW"
                     /locus_tag="OO515_002317"
     CDS             12820..13944
                     /gene="yeaW"
                     /locus_tag="OO515_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6576239.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14000..14965
                     /gene="yeaX"
                     /locus_tag="OO515_002318"
     CDS             14000..14965
                     /gene="yeaX"
                     /locus_tag="OO515_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6576240.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="OO515_002319"
     CDS             complement(15019..16134)
                     /gene="rnd"
                     /locus_tag="OO515_002319"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6576241.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="OO515_002320"
     CDS             complement(16216..17901)
                     /gene="fadD"
                     /locus_tag="OO515_002320"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6576242.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="OO515_002321"
     CDS             complement(18106..18687)
                     /gene="yeaY"
                     /locus_tag="OO515_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6576243.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="OO515_002322"
     CDS             complement(18727..19422)
                     /gene="tsaB"
                     /locus_tag="OO515_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6576244.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19480..21390)
                     /locus_tag="OO515_002323"
     CDS             complement(19480..21390)
                     /locus_tag="OO515_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6576245.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21522..21866
                     /locus_tag="OO515_002324"
     CDS             21522..21866
                     /locus_tag="OO515_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6576246.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5443 a         5698 c         5403 g         5691 t
ORIGIN      
        1 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
       61 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
      121 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
      181 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
      241 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
      301 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
      361 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
      421 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
      481 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
      541 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
      601 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
      661 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
      721 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
      781 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
      841 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
      901 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
      961 cgaatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     1021 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     1081 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     1141 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     1201 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     1261 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     1321 caacatatcg cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa
     1381 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     1441 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     1501 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     1561 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     1621 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     1681 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     1741 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
     1801 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     1861 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
     1921 atgctggata aatccgttat tttgaaaacc acggattatt tgcgcattgc gcaaccagaa
     1981 ctgctcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
     2041 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
     2101 ttgatcgttc atagcatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac
     2161 aaatggcagt ttgatgacaa ttcgcctgat tttttatgca aaaaacgtaa agattttcta
     2221 cttccttcct gcagcaaacg taaagtaagc aggcttatta ttttttggca aggaaaccac
     2281 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
     2341 taacaccact gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag
     2401 cacctttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
     2461 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt
     2521 tctgaactgg aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg
     2581 gcgcggcgtg accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac
     2641 tgttttaggc gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct
     2701 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
     2761 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
     2821 gcaactttct gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac
     2881 tgctgacgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg cgcaagattg
     2941 cgttcactca tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct
     3001 ttcgccatca tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc
     3061 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc
     3121 ggctcagcac gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag
     3181 ggcgatattt tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac
     3241 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc
     3301 caaaccgcat attgcggcgg caccatccat aaagcatttt ccaccgtaca ggtaattgca
     3361 ccatgtaatg ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
     3421 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
     3481 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
     3541 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
     3601 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
     3661 tgatgattgc cacaacatta cgcgtcacct ttatcggcgc agcaccgtta ctggatacga
     3721 ttcgttccgc ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat
     3781 tggcctttgc gctaatctca cctttggcca ccccggtagc gcgacgtttt ggtatggaac
     3841 gtagcctgtt tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt
     3901 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg
     3961 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
     4021 catattccct gacaatggga gctgcagcgg cactgggatc ggctatgata gtgccgctgg
     4081 ctttgaacgg ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg
     4141 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc
     4201 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggta acattgtttc
     4261 ttgggatcaa ctcactgatc tattacgtga ttattggctg gcttccggcg atcctcatca
     4321 gtcacggcta tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
     4381 cagctcccgg tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg
     4441 cagcgttcgt tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc
     4501 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
     4561 tgacgttcat tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg
     4621 cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg
     4681 atgctaacgg tgactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg
     4741 cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt
     4801 gacgtgacaa atcatgcgtg aaagggagaa caaacactat gaatattcag tgcaaacgcg
     4861 tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc
     4921 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
     4981 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
     5041 atcttgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
     5101 aacagccgct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc
     5161 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
     5221 gccggatggt cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
     5281 caattgttgc tgaccccttg cgctgtcgac actggaatga gcgtcccgcc cttctgctgg
     5341 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtac ttctggctga
     5401 gaagaggttg aacaaccagc caggaccagc aagcaaggca aagcaaaact gataattttc
     5461 atttattgat ctcactcatt tatctacgct tagagtatcg cggtatcgtt ttgttttgca
     5521 gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct ttgttggtga
     5581 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
     5641 tttcgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact
     5701 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
     5761 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
     5821 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
     5881 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
     5941 tgcggcacgc aatcttggca tctccacatt tctgagcaca ccaattcact taccggatgg
     6001 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
     6061 ggaacaggtt ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcact
     6121 ggttgaacag ttgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
     6181 aaccgggttg ccgaatccgc gggcgatttt tgaaaatctg acgacgctgt tttctctcgc
     6241 ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat
     6301 caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
     6361 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt
     6421 tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgca ttcagcagca
     6481 aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg
     6541 catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat
     6601 tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc attcagcgcc
     6661 acattgctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
     6721 tttccggttg taatctttat tacagcggtc gtatttttag catggttttt tattggcggc
     6781 tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga
     6841 ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa
     6901 aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat
     6961 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
     7021 gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac cgactatccc
     7081 cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
     7141 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
     7201 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
     7261 gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg cgccatcaac
     7321 gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat aagaacgcac
     7381 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
     7441 cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg tcacggtata
     7501 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
     7561 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
     7621 ttcaatgttg tgccactttt ctggagggaa cacctcaaac tgccccgctt cgataaggat
     7681 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     7741 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     7801 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     7861 ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa cggtttattc
     7921 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     7981 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     8041 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     8101 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     8161 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     8221 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     8281 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     8341 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     8401 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     8461 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     8521 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     8581 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     8641 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     8701 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     8761 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaata attaatcagg
     8821 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctatcg
     8881 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     8941 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     9001 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     9061 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     9121 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     9181 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     9241 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     9301 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     9361 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     9421 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     9481 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     9541 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     9601 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     9661 ccgccacggc ggcaaaacca gcccggcgag ccaccagcat aaagacgcgc aaatcgttca
     9721 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     9781 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     9841 aatgatgaaa acgatgcgta ttgctgcgat cccgggagat gggattggca aagaagtcct
     9901 tcctgaaggg attcgcgtat tacaggctgc cgctgagcgc tggggcttcg acttgagttt
     9961 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
    10021 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
    10081 ggataccgtt ccggaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
    10141 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
    10201 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
    10261 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
    10321 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
    10381 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
    10441 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
    10501 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
    10561 ggtggcgtcc aatttgtttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
    10621 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
    10681 gcctgtccac ggttccgcgc cggatatcta cgggaaaaat attgctaacc ctatcgccac
    10741 aatttgggcc ggtgcgatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
    10801 agcgcataac ggtattctgg cagcgattga agaagtgatt gctcacgggc cgaaaacacc
    10861 ggatatgaaa ggcagtgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
    10921 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
    10981 tttgcagtta ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
    11041 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
    11101 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
    11161 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
    11221 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
    11281 ccgacgcggc caacacaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
    11341 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
    11401 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
    11461 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
    11521 aaacgcctgg attaaatatc gcaccacgtt cacaacaggc actcgaattt agcgtccctt
    11581 actccttctt ccactggggc atcagcacct gggcaactta tacgctggcc tcattaatca
    11641 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
    11701 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgtttctga
    11761 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
    11821 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
    11881 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
    11941 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggtc
    12001 caaccgaatt tattaccaac agcatcatca atgccatcgg cctgaccacg caaaacttcc
    12061 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
    12121 ttttctactg gctgtggtgg atctcatacc cccctggcgt agcaatgttt gtcacccgcg
    12181 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
    12241 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
    12301 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
    12361 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
    12421 tccttgcctc gcatatggat gcagtggcct acaccatggc ggcgaccagt acgcgtaatc
    12481 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
    12541 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
    12601 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
    12661 ggctgaaaca ggattatgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
    12721 caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta ttcaaaggcg
    12781 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
    12841 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
    12901 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
    12961 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
    13021 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
    13081 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
    13141 acctgcccat atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
    13201 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggag
    13261 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
    13321 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
    13381 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
    13441 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgc
    13501 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
    13561 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
    13621 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
    13681 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
    13741 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
    13801 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
    13861 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
    13921 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
    13981 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
    14041 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
    14101 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccagta
    14161 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
    14221 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
    14281 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
    14341 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagtt
    14401 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
    14461 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc atcattcgtc
    14521 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
    14581 ttatacctgt ggccccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
    14641 catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
    14701 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
    14761 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
    14821 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
    14881 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
    14941 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
    15001 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
    15061 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
    15121 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
    15181 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg ctttacgata
    15241 acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
    15301 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
    15361 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
    15421 ctcttcacgc acgacaaagt tcaccgccag atcacgctca cgagccttgc gcagtcgcca
    15481 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg cattggtgat
    15541 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
    15601 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
    15661 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
    15721 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
    15781 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
    15841 gtcaatcagg ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
    15901 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
    15961 gtcggtgatc ccgagaggat caattagcgc cagatgctca ccatcgaaaa gttgaatcaa
    16021 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatcctggg ctatcgccgg
    16081 aaagacacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    16141 attcaaattg tattctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    16201 cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    16261 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    16321 cggtactttg tagcccgtga gctgacggca gcaaaaggtc accagtgact cttcggtaag
    16381 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    16441 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    16501 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
    16561 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
    16621 ctcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc ctttgacaca
    16681 aagctcaccc ggttgacccg gtggtacttc attatcgtcg tcatccacca gtttggcttc
    16741 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
    16801 gctgaccagc ggcgcacact cggtaagacc atagccttcc agcagatact gtcctgtcag
    16861 tttcacccaa cgctctgcca ccacttgctg cactggcatc ccaccgcctg cggaaagatg
    16921 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    16981 gttaacgcca gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    17041 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    17101 aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt actaccagtt ctttacctgg
    17161 atgcagcaac ggcccatagg tggcgttgac ctgttccagg ttcgccagca tattgcggtg
    17221 agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca ggaaggctaa
    17281 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    17341 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    17401 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    17461 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    17521 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    17581 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    17641 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    17701 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    17761 ctcccccata ttcacaaacg caggctgatc ggcgtagcgg acgaccgact gctcaaacat
    17821 atctatcaga gattgataac ggtcagggtt gatctccgtc ggaacatccg cgggataacg
    17881 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    17941 cccaagtgat aaacatgttg ttaacataac attaactcat cttaccagct tgataattag
    18001 acaacgaaaa ggttgcgaag cgcgtcacta tttattttta tagttgccgt atgaacgcag
    18061 aaacagcgga ccagccgctg tttctttttc tttaaaaaca acttgttact cggttacaat
    18121 tgtttcaact cttgcagggc caggattata ccaaccccat ccgccgtaac catagggcca
    18181 gccgcgtcca ccataaaacc atggatcaat tggctgcggc ggcataatca cctgttgagt
    18241 gatatgccag cgtttgtaac ctgttacctg cataaccata aatttatatg gtgcattatt
    18301 tactttgcca tccactgtgc cagtgattgg cccgacaacc gtcaccagtt gtccacgaaa
    18361 atcgaccgga tcaagaaaac cgttcacatc ggcataaatg cgcccgcgtg aaggttctcc
    18421 caaggttggt cttgctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    18481 ttgctggttt tgtaccacaa ccactttgcc gccaaagcgc gcctcctgac caacatataa
    18541 atctggcgca ttcatcacgc gtaccagatc ttgctgtggt gttggactgc tgcctttaat
    18601 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    18661 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    18721 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    18781 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    18841 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    18901 acggagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    18961 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    19021 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    19081 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccctgcgcca tcgtcattag
    19141 cgtagagaca ccaatcatcg gtaactccgc gccaagcgcc agcccttgcg caataccaat
    19201 accaatgcgc acgccagtaa agctaccggg gccgcgcccg taagccagag cgtttatgtc
    19261 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    19321 atgttcacga gggcaaagct caaaatgagc gttgacatta ccgtcgttcc acagagcgac
    19381 agagcagacc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    19441 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    19501 aaggaaacga accgcacggg caatgtcacg ggtgcgtggc gcgggcggca gactggcgag
    19561 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaattacca gcacgccgcg
    19621 atcgtcggcg tcgcgaatca gtcgccctac tccctgcttg agagtaatga ccgcatccgg
    19681 cagctgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    19741 taacagtaga tcatccggcg aggtaaacgg caatttgtcg ataattacca atgacaatgt
    19801 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    19861 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    19921 aagcgtcatg gtagcgcgga actgctcggc cagatcgcgc atcatggcat gcgaggtaca
    19981 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgccagttg
    20041 ccgtgcacaa cccggttgat tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg
    20101 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    20161 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    20221 accgggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    20281 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    20341 ttttagccgc ttcagccgtg tgcgatacaa cgtggcgcgc tcaaatgccg catccagcaa
    20401 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    20461 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    20521 acctggctca ccgagttgca gacgaaaatc ctgtgcactc tgggcaagac gatcagcgca
    20581 cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga tgtcttttgc
    20641 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    20701 tagctggtgg gcttcgtcga agatcatgac gtccgcttca gggatcaact cgccaaatcc
    20761 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    20821 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    20881 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    20941 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    21001 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    21061 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    21121 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    21181 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    21241 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    21301 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    21361 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    21421 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    21481 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    21541 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    21601 taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacacgctg gcacagattg
    21661 acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    21721 tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg
    21781 gtcatgcgcc agtgcgctgc acggtacaag cgggtttgat gaacccgaag tataaagttg
    21841 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    21901 tatcgataat actggtcggt tttacataaa tcgaccagag aataagatat tactgttgat
    21961 gcttctgcta attcattatt tatatttata atttcaattt tatctataaa actaactata
    22021 taaataaaca taaccatgcc gttgtgttcg ccctttgatt agcaactctg gttttctttc
    22081 ttagctgaac aatctggtta tagcgtgcgg actgaccgtt aattttctcc ttcttactgg
    22141 ataacacacc atgagctgct acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc
    22201 atatcgttat aatccctttg atattagatg caaat
//
LOCUS       DAJTXQ010000064        22226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000064 DAJTXQ010000000
VERSION     DAJTXQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.064"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO515_002325"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO515_002325"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6576247.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO515_002326"
     CDS             complement(1672..2730)
                     /locus_tag="OO515_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6576248.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO515_002327"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO515_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO515_002328"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO515_002328"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6576249.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO515_002329"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO515_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6576250.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO515_002330"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO515_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6576251.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO515_002331"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO515_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6576252.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO515_002332"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO515_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6576253.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO515_002333"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO515_002333"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6576254.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO515_002334"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO515_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6576255.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO515_002335"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO515_002335"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6576256.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO515_002336"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO515_002336"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6576257.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO515_002337"
     CDS             complement(17699..18322)
                     /locus_tag="OO515_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6576258.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="OO515_002338"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="OO515_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR6576259.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO515_002339"
     CDS             complement(18971..19213)
                     /locus_tag="OO515_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6576260.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO515_002340"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO515_002340"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6576261.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="OO515_002341"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="OO515_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6576262.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="OO515_002342"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="OO515_002342"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6576263.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         6271 a         5189 c         4837 g         5929 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgctg ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctca
//
LOCUS       DAJTXQ010000065        22132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000065 DAJTXQ010000000
VERSION     DAJTXQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.065"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..525)
                     /locus_tag="OO515_002343"
     CDS             complement(<1..525)
                     /locus_tag="OO515_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6576264.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            821..1597
                     /gene="exuR"
                     /locus_tag="OO515_002344"
     CDS             821..1597
                     /gene="exuR"
                     /locus_tag="OO515_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6576265.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1942..2604
                     /locus_tag="OO515_002345"
     CDS             1942..2604
                     /locus_tag="OO515_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6576266.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2608..2991
                     /gene="mzrA"
                     /locus_tag="OO515_002346"
     CDS             2608..2991
                     /gene="mzrA"
                     /locus_tag="OO515_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6576267.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3138..3506
                     /locus_tag="OO515_002347"
     CDS             3138..3506
                     /locus_tag="OO515_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6576268.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3544..3849
                     /locus_tag="OO515_002348"
     CDS             3544..3849
                     /locus_tag="OO515_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6576269.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3852..4256
                     /locus_tag="OO515_002349"
     CDS             3852..4256
                     /locus_tag="OO515_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6576270.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4246..4545
                     /locus_tag="OO515_002350"
     CDS             4246..4545
                     /locus_tag="OO515_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6576271.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4731..5123
                     /locus_tag="OO515_002351"
     CDS             4731..5123
                     /locus_tag="OO515_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6576272.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5193..6206
                     /gene="yqjG"
                     /locus_tag="OO515_002352"
     CDS             5193..6206
                     /gene="yqjG"
                     /locus_tag="OO515_002352"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6576273.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6471..6836
                     /locus_tag="OO515_002353"
     CDS             6471..6836
                     /locus_tag="OO515_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6576274.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7078..7434
                     /locus_tag="OO515_002354"
     CDS             7078..7434
                     /locus_tag="OO515_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6576275.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OO515_002355"
                     /pseudo
     CDS             complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OO515_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8486..9187
                     /locus_tag="OO515_002356"
     CDS             8486..9187
                     /locus_tag="OO515_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6576276.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9210..9374
                     /locus_tag="OO515_002357"
     CDS             9210..9374
                     /locus_tag="OO515_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576277.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9508..10818)
                     /locus_tag="OO515_002358"
     CDS             complement(9508..10818)
                     /locus_tag="OO515_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6576278.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10846..12177)
                     /locus_tag="OO515_002359"
     CDS             complement(10846..12177)
                     /locus_tag="OO515_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6576279.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OO515_002360"
     CDS             complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OO515_002360"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6576280.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13888..14277)
                     /locus_tag="OO515_002361"
     CDS             complement(13888..14277)
                     /locus_tag="OO515_002361"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6576281.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OO515_002362"
     CDS             complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OO515_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6576282.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OO515_002363"
     CDS             complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OO515_002363"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6576283.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OO515_002364"
     CDS             complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OO515_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6576284.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OO515_002365"
     CDS             complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OO515_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6576285.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OO515_002366"
     CDS             complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OO515_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6576286.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21421..21765
                     /gene="tdcR"
                     /locus_tag="OO515_002367"
     CDS             21421..21765
                     /gene="tdcR"
                     /locus_tag="OO515_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6576287.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5801 a         5649 c         5169 g         5513 t
ORIGIN      
        1 gcgttgattc cctgcattaa ctaatttttg ggcagcattt acttctaact tagcaatagg
       61 ttgctgaggt ggaacactaa caacaatccc ccaaattaag ttaactgtta actgcataga
      121 gagatctttt ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg
      181 ataatttttt tctttttgct ctggttccat tgcaacaaag cgcacagttt tgctactacc
      241 ggctggtaaa gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat
      301 ctcaacttca ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat
      361 gtactgaact tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat
      421 ggaaacagcc ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa
      481 taaaagcggc gtaagccgtg ataagagatt aattttcgcc accatataaa tttgatctta
      541 caatattgtt tccaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa
      601 aaaagcattt ttagttaata ttaaatttaa aggctgggaa ttaacttact gaaatttagt
      661 agctttcaca atgacgatac ttatttttta ggaatttata aatattcaca atattaattc
      721 aatacattaa ttcaatcacc aaatttatat atgaccaatg gcatacccat gaataaagtg
      781 gtatgataac ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg
      841 ccgtttgtat caacaacttg ccgctgacct gaaagagcgc attgaacagg gcgtctatct
      901 ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac
      961 ggtagttcgt gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg
     1021 ttcaggtatt catgtggttt ccaaccagcc gcgccatcag caggcggctg acaataatat
     1081 ggaattcgcc aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa
     1141 tattgccgag ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat
     1201 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
     1261 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
     1321 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
     1381 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
     1441 ccatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
     1501 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctattcgccg aaaacccggt
     1561 agtgcatctc gatacggcca ccagtggcag taaatgaaac tcctgtccga caggcgctgc
     1621 gacgcgcctg ttcgcttcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
     1681 tctcgccacc ctcccctgca ttcagcaaaa tcagactcca cggacatgta attttgataa
     1741 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgttt ttgaatgctt
     1801 tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
     1861 acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa
     1921 ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg
     1981 caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt
     2041 gtaattttgt tccttgaaaa cggcttgctt ccagcggcct ttttaccggg cgacagttta
     2101 ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg
     2161 ctgctgaccg ttgccgccag cctcggctgc tgggtcagct atattcaggg gcgatggctg
     2221 ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc
     2281 gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgcg
     2341 tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt
     2401 cagtttttca actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac
     2461 atgctcggca aaacgccgat atttttaaag tacgaggacc agctgatgtc atgcctgatg
     2521 ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa
     2581 aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta
     2641 gcctggtccg gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt
     2701 cgccagcaag agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac
     2761 ggtttttcga tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc
     2821 aaaaacgaca ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg
     2881 gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag
     2941 gctatgcagt ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt
     3001 atccgaaaca tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg
     3061 gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca
     3121 caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta
     3181 gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga
     3241 tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga
     3301 gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg
     3361 caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag
     3421 gcgatgccga taagattgcc aaacgcgaac ggaaactggc agaagcgcag gaagagctga
     3481 aaaagctgga agcgcgcgac tactaactca caatagtcac tacttactca cctggagaaa
     3541 actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc cctttccgat
     3601 acgctggaag aggtgcttag ctcatctggc gagaagtcga aagaagagtt gagtaagatt
     3661 cgtagcaaag cggagcaggc actgaaacag agccgttatc gcctgggtga aaccggtgat
     3721 gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg atgagtatgt gcgtgaaaat
     3781 ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag tgctcggcgt tctgctgtcg
     3841 cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc gttctgggca
     3901 tcgggcagcg aattgtttcc atcatggttg aaatggtgga aacacgtctg cggctggcgg
     3961 tggtggagct ggaagaggaa aaagctaatc tctttcaact tttactgatg ctgggcctga
     4021 cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatt tgggcggttg
     4081 atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg ctactggcac
     4141 tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc caccttgctg cgccatacac
     4201 gccatgagct ggcaaacgat cggcaactgc tcgaggagga gtcccgtgag cagtaaagtc
     4261 gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg gctggatctt
     4321 tccgccagtc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg ctggaatatg
     4381 ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggcgat ctggacgatt
     4441 cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggcgtatg gagcgcctgg
     4501 cgtctggtta aaacgaccct caagcagcaa cagcttcgcg gttaacttcc ctctggccgg
     4561 agcaattccg gccttatccc tcaaattttt tgaagatttt tgacagtttt ccttgctaac
     4621 aatcatcatt caccacgttt atgattctct ccatcgacag caacgacgct aataccgcgc
     4681 ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt ggagagtatg atgaaaaaat
     4741 tagaagatgt tggtgtactg gtagcgcgca ttttaatgcc gattctgttt attaccgcag
     4801 gctggggaaa aatcaccgct tactctggta cccaacaata tatggaagca atgggtgtcc
     4861 cgggttttat gctgccgctg gtgattctgc ttgagttcgg tggtggtctg gcaatcctgt
     4921 tcggtttcct gactcgcacc acagccctgt ttactgcggg ctttacgctg ctgacggcat
     4981 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     5041 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     5101 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     5161 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     5221 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     5281 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     5341 cagagaaaga tcgttatcat ctctatattt cactcgcctg cccgtgggcg caccgcacgc
     5401 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     5461 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     5521 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     5581 gagttactgt tcccgtactg tgggacaaaa agaaccacac catcgtcagc aacgaatcaa
     5641 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcgaaa gcgggtgatt
     5701 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     5761 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     5821 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     5881 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     5941 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     6001 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     6061 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     6121 caattggtcc gtggcaggat ctcgatgaac cgcaggacga gatgttcgct tcggttaaaa
     6181 aaaggcacct tacgaagtgc ctctgataat tagctaagtg catgttgttc atatcggatg
     6241 cggcgtgaac gccttatcaa acctacaaaa catttatatt caatatgttg caatgaccgc
     6301 gcaggccaga taagcgtagc gcatctggcg attttgcgct tgtcatcagt cttagcgcgc
     6361 cttcacagca ctttattaat tccttgaacg aatatttact gccatttcat tcacgtctat
     6421 tcttaatttg ctgctttaaa aacaagtgat tgagcaacat tgaggcaaaa atggactggt
     6481 atctgaaagt aataaaaaat tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact
     6541 ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctgctg gataaaatgt
     6601 taggctggca acgcgctggt ggcgaaggca tcctgacgac aatctacggt attctggtgt
     6661 ttttaccgtg gtgggcagtt cagttccgcc gcctgcacga caccgaccgc tcggcgtggt
     6721 gggcactgct attcttaatc ccgtttatcg gctggcttat tatcatcgtc tttaactgcc
     6781 aggcgggtac gccaggcgaa aaccgctttg gacctgatcc aaaactggaa ccttaaaaca
     6841 aatctgctcg ccggatgtct gactcatccg gcttttatca tcatgttgat gaatgaataa
     6901 tttactaatg tttatttaaa atatttcaat gtcgttatat aaattaccta taaaaaataa
     6961 ccatgtattt atcttaaata tcaaaacctt tataacatcc gaaaaagaaa gatacgaata
     7021 gcatttacac aatacccgga atagactttc gtcactttta attaaaggga tgtttttatg
     7081 cagtggtatt tatccgtatt aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa
     7141 gagtactgga tgtttactct gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa
     7201 ttgattttag gtctggagct tccatatctg tctatgcttt atttgctggc aaccttcctt
     7261 cccgtccttg cgcttgctat ccgtcgctta cacgataccg accgttcagg tgcctgggcc
     7321 ttgctctttt ttgttccgtt tattggctgg ctcgtgcttc tggttttttt ctgcacagaa
     7381 ggtacttctg gcagcaatcg ttacggaaac gatccgaagt ttggttcaaa ttaatcttag
     7441 aattggggcg atatttcgcc cctttttatt aacaataata aatattattt tccgctaaaa
     7501 agtttgggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag
     7561 gccataataa tatcgatctc actggtcgat tccgggctga cgacacgcaa ccgcccttcc
     7621 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
     7681 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagttgt
     7741 acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg atatttcacg
     7801 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
     7861 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc
     7921 atatccggcg cgataacaat atccgcccgc ccctgctcca gtcgttccta cgcccccgcc
     7981 agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
     8041 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
     8101 ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgc
     8161 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
     8221 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
     8281 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
     8341 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
     8401 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
     8461 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
     8521 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
     8581 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
     8641 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
     8701 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
     8761 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
     8821 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
     8881 actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta cgatgggaag
     8941 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     9001 gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     9061 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     9121 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     9181 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     9241 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     9301 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     9361 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     9421 atccaacaac attaacgaat taaacaaccc gtaggccgga taaggtgcgc cagcatcgca
     9481 tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     9541 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     9601 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     9661 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     9721 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     9781 agtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     9841 cgttgcggca caaagtgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     9901 atgagaaagc atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt gttctgctac
     9961 aaccaccaca ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc
    10021 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
    10081 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
    10141 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
    10201 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
    10261 cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc
    10321 ctgctgggta aacaccacac cattgtgcgt ctcaatacgc acaatgttgg tatgcccgcc
    10381 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
    10441 ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
    10501 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
    10561 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
    10621 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
    10681 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
    10741 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
    10801 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
    10861 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
    10921 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
    10981 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatagc
    11041 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
    11101 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
    11161 aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
    11221 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
    11281 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgc cccatcgcca gcgtgaacga
    11341 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
    11401 tgctttatgc cgcgccactt caatagattt ttcccgcgag cgataagaga tcaccatcgg
    11461 gcttaacgtc tggataaaca aaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
    11521 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
    11581 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat
    11641 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
    11701 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga
    11761 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
    11821 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
    11881 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
    11941 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
    12001 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
    12061 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
    12121 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcga tttccataat
    12181 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
    12241 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
    12301 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
    12361 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
    12421 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
    12481 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
    12541 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccacgcgg
    12601 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
    12661 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
    12721 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
    12781 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
    12841 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
    12901 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
    12961 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
    13021 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
    13081 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
    13141 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aatcctgcgt
    13201 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
    13261 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
    13321 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
    13381 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
    13441 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
    13501 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
    13561 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
    13621 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca
    13681 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
    13741 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
    13801 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
    13861 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
    13921 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
    13981 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
    14041 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
    14101 cgctttgacg ttttcgaggc ttaaacacgc ctgatcttgc acatcagccg ggatctcacc
    14161 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
    14221 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
    14281 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
    14341 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
    14401 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
    14461 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
    14521 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
    14581 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
    14641 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
    14701 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
    14761 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
    14821 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
    14881 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
    14941 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
    15001 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
    15061 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
    15121 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
    15181 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
    15241 acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
    15301 ccattgggct gacgcaacaa gcaattgcgt aatcgtcgct gttgaagtca gtacgcatca
    15361 gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
    15421 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    15481 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
    15541 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
    15601 caaattccgg cgtacgcaag aagcgcacca tacggatctt cataatgaag tgatcgatca
    15661 gttcctgtgc ctgctgctca ttgagtacgc ctgctttaaa gtcgcgctca atgtagatat
    15721 cgaggaacga cgccgtgcgc cccagcgaca tcgcgccgcc gttctgtgat ttcactgccg
    15781 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
    15841 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
    15901 ccagctcctc acgcagacgg atggtggctt ccagatcttc gcctttttcc agacgggact
    15961 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
    16021 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
    16081 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
    16141 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
    16201 cgtggaatga acttttaatc atattaatgc caccgaacgg atgcagcgcg cgctttaatg
    16261 gcgcatccgt ttgcagacca acaatttttt ccagcggctg gttaatatat cccgcatcat
    16321 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
    16381 cgatacggat gccttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
    16441 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
    16501 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
    16561 cgctggtatc aatatctatc ttcatgaaaa ataatctctc tacaatactt caactaaatt
    16621 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
    16681 tttcttcgtt agtcggaata acggcacaaa tgacacgcgc attttcactg gaaacaattc
    16741 gctctccaaa agagttagag cgattattca tttctgtatc aatcactacg cctaatacag
    16801 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
    16861 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
    16921 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
    16981 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    17041 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    17101 caaagtcgac atcgccactg cgggttccca tcatcaagcc ttccagcggc gtcattccca
    17161 ttgaagtatc aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat
    17221 gcgccacaac caggccggag tcatcttccg ccagattcag cagagaatgt gcgcgctggg
    17281 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    17341 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    17401 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    17461 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    17521 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    17581 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    17641 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    17701 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
    17761 cgagcacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    17821 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    17881 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    17941 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    18001 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    18061 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
    18121 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    18181 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    18241 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    18301 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaat
    18361 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    18421 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    18481 ttagaaacca cgaaggaaga gacgattggc gagaaattaa aggagaaaac catgatggaa
    18541 atccccagcc agacggtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    18601 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    18661 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    18721 aaccagatga cgaaagccat cagcagcagc aggaacagcg ccacaaagcc gcgattcagc
    18781 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    18841 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    18901 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    18961 agacatagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    19021 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    19081 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    19141 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    19201 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    19261 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    19321 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    19381 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    19441 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    19501 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    19561 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    19621 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    19681 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    19741 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    19801 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    19861 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    19921 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    19981 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    20041 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    20101 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
    20161 tcaatagcaa tcggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    20221 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    20281 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    20341 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    20401 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    20461 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    20521 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    20581 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    20641 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    20701 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    20761 tttctgcact taatgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    20821 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    20881 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    20941 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    21001 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    21061 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    21121 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
    21181 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    21241 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    21301 gaccactcac aaattaactt tcaatacttt ccagggtatc gtcattacaa ttaaatacct
    21361 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt gtcaaaaaaa
    21421 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
    21481 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
    21541 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
    21601 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
    21661 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
    21721 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
    21781 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct gatcgccgcg
    21841 ataaggtcag acaaagacaa caagggaaat tttcacagag cttttgatcg tcgtaggcca
    21901 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgaaa
    21961 gaaccctgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
    22021 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
    22081 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tg
//
LOCUS       DAJTXQ010000066        21998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000066 DAJTXQ010000000
VERSION     DAJTXQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.066"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OO515_002368"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OO515_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6576288.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OO515_002369"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OO515_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6576289.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OO515_002370"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OO515_002370"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6576290.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="OO515_002371"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="OO515_002371"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6576291.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OO515_002372"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OO515_002372"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6576292.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OO515_002373"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OO515_002373"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6576293.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OO515_002374"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OO515_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6576294.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8253..8468
                     /locus_tag="OO515_002375"
     CDS             8253..8468
                     /locus_tag="OO515_002375"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576295.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="OO515_002376"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="OO515_002376"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6576296.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="OO515_002377"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="OO515_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6576297.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="OO515_002378"
     CDS             11833..12330
                     /locus_tag="OO515_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6576298.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="OO515_002379"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="OO515_002379"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6576299.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="OO515_002380"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="OO515_002380"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6576300.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="OO515_002381"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="OO515_002381"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6576301.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="OO515_002382"
     CDS             15015..15578
                     /locus_tag="OO515_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6576302.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="OO515_002383"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="OO515_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6576303.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="OO515_002384"
     CDS             complement(16169..17149)
                     /locus_tag="OO515_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6576304.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="OO515_002385"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="OO515_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6576305.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="OO515_002386"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="OO515_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6576306.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /locus_tag="OO515_002387"
     CDS             18452..18742
                     /locus_tag="OO515_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6576307.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18750..19343
                     /locus_tag="OO515_002388"
     CDS             18750..19343
                     /locus_tag="OO515_002388"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6576308.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="OO515_002389"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="OO515_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6576309.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21769
                     /locus_tag="OO515_002390"
                     /pseudo
     CDS             20783..21769
                     /locus_tag="OO515_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            21814..>21998
                     /locus_tag="OO515_002391"
     CDS             21814..>21998
                     /locus_tag="OO515_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6576310.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5443 a         5716 c         5596 g         5243 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga taaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga
    21721 tccaaaacaa gctgctttgt tagagaagtt cgacaacgct gctcaataat attccgggcc
    21781 agtctctggc cctgattttc caagatgctt acaatgatat ttaaccgcct gaaacttgcg
    21841 gtagaccgcg tcattgccgc gttttctgtc gccgtcatgc ttgctctggt tgtatgcgta
    21901 gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac cgatgagctg
    21961 gcacgatttt tgatgatctg ggtagggtta ttaggtgc
//
LOCUS       DAJTXQ010000067        21641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000067 DAJTXQ010000000
VERSION     DAJTXQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.067"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="OO515_002392"
     CDS             complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="OO515_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6576311.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1343..2995
                     /gene="ushA"
                     /locus_tag="OO515_002393"
     CDS             1343..2995
                     /gene="ushA"
                     /locus_tag="OO515_002393"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6576312.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="OO515_002394"
     CDS             complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="OO515_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6576313.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3715..4509)
                     /locus_tag="OO515_002395"
     CDS             complement(3715..4509)
                     /locus_tag="OO515_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6576314.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4647..4988
                     /locus_tag="OO515_002396"
     CDS             4647..4988
                     /locus_tag="OO515_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6576315.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5203..7707)
                     /gene="copA"
                     /locus_tag="OO515_002397"
     CDS             complement(5203..7707)
                     /gene="copA"
                     /locus_tag="OO515_002397"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6576316.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7969..8901
                     /gene="glsA"
                     /locus_tag="OO515_002398"
     CDS             7969..8901
                     /gene="glsA"
                     /locus_tag="OO515_002398"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6576317.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8904..10196
                     /locus_tag="OO515_002399"
     CDS             8904..10196
                     /locus_tag="OO515_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6576318.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10321..10728
                     /gene="cueR"
                     /locus_tag="OO515_002400"
     CDS             10321..10728
                     /gene="cueR"
                     /locus_tag="OO515_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6576319.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10729..11187)
                     /locus_tag="OO515_002401"
     CDS             complement(10729..11187)
                     /locus_tag="OO515_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6576320.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11184..12101)
                     /locus_tag="OO515_002402"
     CDS             complement(11184..12101)
                     /locus_tag="OO515_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6576321.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12247..12924
                     /gene="fetA"
                     /locus_tag="OO515_002403"
     CDS             12247..12924
                     /gene="fetA"
                     /locus_tag="OO515_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6576322.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12911..13690
                     /gene="fetB"
                     /locus_tag="OO515_002404"
     CDS             12911..13690
                     /gene="fetB"
                     /locus_tag="OO515_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6576323.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="OO515_002405"
     CDS             complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="OO515_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6576324.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="OO515_002406"
     CDS             complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="OO515_002406"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6576325.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="OO515_002407"
     CDS             complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="OO515_002407"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6576326.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16061..16747
                     /locus_tag="OO515_002408"
     CDS             16061..16747
                     /locus_tag="OO515_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6576327.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16744..19158
                     /locus_tag="OO515_002409"
     CDS             16744..19158
                     /locus_tag="OO515_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6576328.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="OO515_002410"
     CDS             complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="OO515_002410"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6576329.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20555..21481)
                     /gene="allS"
                     /locus_tag="OO515_002411"
                     /pseudo
     CDS             complement(20555..21481)
                     /gene="allS"
                     /locus_tag="OO515_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5683 c         5798 g         5145 t
ORIGIN      
        1 atcggcgata agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa
       61 aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat
      121 ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg
      181 caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat
      241 cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac
      301 ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg
      361 catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat
      421 gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat
      481 cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg
      541 cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc
      601 ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc
      661 gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg
      721 agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac
      781 tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc
      841 aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca ctggttgcag
      901 tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga
      961 aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag
     1021 cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga
     1081 cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa agtcactttt
     1141 tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt
     1201 atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt
     1261 tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg
     1321 aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt
     1381 aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa
     1441 aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga
     1501 atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga
     1561 aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga
     1621 cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc
     1681 gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc
     1741 caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa
     1801 accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga
     1861 tgacacagca aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc
     1921 cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat
     1981 cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga
     2041 tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc
     2101 gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac
     2161 gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa
     2221 atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca
     2281 gctgattccg gtgaacctga agaagaaagt gaccagggaa gacgggaaaa gcgagcgcgt
     2341 gctgtacacc cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca
     2401 gaacaaaggc aaagcgcagt tggaagtgaa aataggcgaa accaatggtc gtctggaagg
     2461 cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca
     2521 aatggatcgc actggtgccg actttgcggt gatgagtgga ggaggaattc gtgattctat
     2581 cgaagcaggc gatatcagct ataaaaacgt actgaaagtg cagccattcg gcaatgtggt
     2641 ggtgtatgcc gacatgatcg gtaaagaggt gattgattac ctgaccgccg tcgcacaaat
     2701 gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg
     2761 caaactcaac gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat
     2821 ggcgacatta aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc
     2881 gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag
     2941 ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga
     3001 aagtgccgga tatttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt
     3061 tggcatcgag gatcttcgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc
     3121 cgccagaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gtcggcagac
     3181 gttttttctg ccccaatggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg
     3241 ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat
     3301 ccagttgacc tgcgaccggc gtaacggcca ctgccagatg tttcatatca ccgttcactg
     3361 ccaccagtag cgttttgtag acttgatccg gattcaaacc taattttttg acgacttcat
     3421 cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt
     3481 ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac
     3541 gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg
     3601 gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat
     3661 aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg
     3721 caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata
     3781 tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag
     3841 cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggagg
     3901 tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt
     3961 cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggtaatctg
     4021 gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaataa
     4081 ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag
     4141 aaccatcgcg atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc
     4201 ctggctaatg tgctgcagat tttggagttg ctcctcgtta atgcgctctt ccagcgcctc
     4261 gcaagtaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag
     4321 cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct
     4381 acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg tgatatcgat
     4441 ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa
     4501 cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca
     4561 aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat
     4621 tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg
     4681 gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag
     4741 agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat cgtaaactca
     4801 ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa
     4861 acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat
     4921 tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg
     4981 tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc
     5041 cggcatccga gcaccattgc ctgatgcgac gcttacccgt cttatcaggc ctacaaacct
     5101 gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg
     5161 ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa
     5221 ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc
     5281 ggcaactacc gggttaagca gtgttcccgt aaacggccac aaaataccgg cggcgaccgg
     5341 aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt
     5401 tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat
     5461 cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg
     5521 ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact
     5581 ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc
     5641 aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac
     5701 cagacgatat cccgttttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg
     5761 atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc
     5821 tccttgcgat gcctgagcag taatctccgc ttcgatagct ttggtaccaa cctgttgctc
     5881 atttaacagc gcctgattgc ccagcaataa cgcatgacct tcagcttcac cgctcacgcc
     5941 cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac ctgctttatc
     6001 gaggatcgcc cgtgccagcg gatggctgga accttgctcc agtgccgccg ccagacgcaa
     6061 tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg acaacctgcg gcttcccttc
     6121 ggtcagcgtc ccggttttat cgaacacgat ggtatcgagt gtactggcgc gttgcagcgc
     6181 gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga
     6241 catcggcgtc gccagcccca gcgcacacgg acaggcgata atcagtaccg tggtggcaat
     6301 caccagggta taaacaattt gcggtgccgg accaaagaag taccagattg ccgcactgac
     6361 cagcgcaatc accaccacca caggtacaaa tacggctgag attttatccg ccagctgacc
     6421 gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccgtg acagcgtggt
     6481 atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc
     6541 atggacgctt tcgccttcgc ctttttgctg tgggattggt tcgcccgtca gcatcgcttc
     6601 atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt
     6661 caggcgcagt aacatccctg gctgaacttc cgccagcggc acgctttttt caccatctgc
     6721 ggtgaccact cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg ccttagaaga
     6781 acgctgacgt gcgcgcgctt ccagcatatg gccgagatta atcagaccga taatcatcgc
     6841 actggcttca tagtaaagat gccgcgcttc catcgggaac cactgcggcc acaggttgac
     6901 gctcatcgaa tagagccacg ccacacccgt tcccagcgcc accagcgtat ccatcgtcgc
     6961 tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat
     7021 cactgccagg gttatcaggc cgataactaa ccacaagctg cggttgtcag cggtgaccat
     7081 catgttatcg ccgatcatcc cccagaccat taccgggata cccacagcca gtgcaacaat
     7141 tgcctgccag cggaagcgct tcatcgtagc tacggcggtt tcttgctggc gctcgcggcg
     7201 tttagcgtca tcttcaatcg cttccgcacc gtagcccgct ttttccaccg cctgcactaa
     7261 atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc
     7321 ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca
     7381 gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag
     7441 cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc
     7501 ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc
     7561 tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgcct gctcaacatc
     7621 cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc
     7681 cagggtcagg tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg
     7741 tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta
     7801 aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt
     7861 tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac
     7921 cggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca
     7981 aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga
     8041 caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct
     8101 atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg
     8161 gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg
     8221 gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta
     8281 gagttgcatg gcggcaaacc gctgtcgcca ctggtaaatg ctggcgctat tgccaccacc
     8341 agcctgatta acgctgaaaa tgttgaacaa cgctggcagc gaattttaca tatccaacag
     8401 caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc
     8461 aacttccata accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat
     8521 gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa
     8581 ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc
     8641 gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat
     8701 ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc
     8761 ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac
     8821 gaagaaggca acagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc
     8881 tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc
     8941 tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg
     9001 cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg
     9061 gcggtattgt ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca
     9121 gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag
     9181 cgctctcgtt gctgtacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt
     9241 ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc
     9301 tctacgcgtt ggggatcatt gcggtgatga cgctttttaa ctccttaagc aaccatgcgg
     9361 tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta
     9421 ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg
     9481 gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg
     9541 cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg
     9601 cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat
     9661 cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc
     9721 tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca
     9781 ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac
     9841 tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaac attattgtcg
     9901 tggtgttgat tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca
     9961 gcgccacctt tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg
    10021 atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg
    10081 ttggctttat ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct
    10141 cactcttact cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc
    10201 tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa
    10261 aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg
    10321 atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat
    10381 gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg
    10441 cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg
    10501 gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc
    10561 aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc
    10621 atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc
    10681 ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac
    10741 ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc
    10801 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
    10861 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
    10921 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
    10981 ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg
    11041 caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
    11101 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
    11161 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc
    11221 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
    11281 actttgctgt tactggaggt accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
    11341 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
    11401 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
    11461 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
    11521 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
    11581 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
    11641 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
    11701 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
    11761 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc
    11821 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
    11881 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
    11941 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
    12001 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
    12061 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
    12121 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
    12181 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
    12241 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
    12301 gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
    12361 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
    12421 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
    12481 taccgccaac aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac
    12541 gataatctga tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc
    12601 gattttctcg aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta
    12661 tctggtggtg aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt
    12721 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
    12781 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
    12841 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
    12901 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
    12961 ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
    13021 ctggagcgtc ggacgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat
    13081 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
    13141 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
    13201 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
    13261 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
    13321 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
    13381 ggagaaactg agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
    13441 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
    13501 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
    13561 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
    13621 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
    13681 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
    13741 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
    13801 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
    13861 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
    13921 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
    13981 cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
    14041 ttgcgccacc atcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac
    14101 cgcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat
    14161 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgaa
    14221 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
    14281 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
    14341 cggttgcccg ttctggaaca gatacaccgt cggaatcgcg cgcagaccaa actgggcggc
    14401 aatcatctgc tccgcatcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
    14461 cgcaaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
    14521 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
    14581 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
    14641 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
    14701 cggcagcagg cgcttaagca ccattactgc ccaagtcacc agcgttaccg gatagcgcat
    14761 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
    14821 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
    14881 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
    14941 gtggcgtagc tccatgcgca gcgcgtctga ccatgcctcc agcgcatatt tactggccgc
    15001 gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgatgtca tcacaatacg
    15061 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
    15121 aaagttggcg gaaaactgct gttccatctg cgcgcggctg atggtggaaa gggggccata
    15181 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
    15241 ttcgtcggct gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca cgccggtaaa
    15301 tcccatgctg ttcatacgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
    15361 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
    15421 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
    15481 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
    15541 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
    15601 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
    15661 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
    15721 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
    15781 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
    15841 tcagcagagc cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat
    15901 taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
    15961 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
    16021 cggcacggaa ggttaacagg gccaggaaca ggaagggcaa atgccagcgg aaaacattgt
    16081 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
    16141 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
    16201 gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt
    16261 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
    16321 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
    16381 aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc
    16441 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
    16501 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt
    16561 gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga
    16621 cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct
    16681 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
    16741 agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg
    16801 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
    16861 gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt
    16921 tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
    16981 aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc
    17041 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg
    17101 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
    17161 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
    17221 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
    17281 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ttataaattc
    17341 ggcggcaacg agaaccagct cgacggctat gataaatggt tgttacctca gcttaaaccc
    17401 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
    17461 tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg
    17521 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
    17581 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
    17641 acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt gttgatggtg
    17701 ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctttggcc gtggctgtgg
    17761 gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg
    17821 ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg
    17881 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt
    17941 ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
    18001 gtgctgggct ggatgcagct gaatgtactt cgccgcatga cgctgaaatc gctacctctg
    18061 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca
    18121 ttttcgctct cctttatgct gctggcgctg ttgctggtat tgcgtggcga tctgctcgac
    18181 cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa catcgccaca
    18241 gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc ggaatcattt
    18301 tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa
    18361 gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
    18421 ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg
    18481 gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag
    18541 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc
    18601 tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc
    18661 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc
    18721 ctgttagata ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
    18781 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
    18841 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
    18901 gggaaaaaac tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttct
    18961 ggcctggtag ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt
    19021 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcactg
    19081 ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc
    19141 aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc
    19201 tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac
    19261 gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc
    19321 ggttttttat atctgtattc tggtgcaacc attctgttct cacgacaatc gccgtttcag
    19381 aataccgccc atcactgcgt cttaacccat tccgccactt ccgcccactc accgcgaaag
    19441 acaacttttt ccgctttttt ctcaagctga tagcgataca tcgggtcgta atattcttta
    19501 agtaacggca ccagccaggc cagatgaccg tcggtgctgt cggtggtgag ttgcgttgtc
    19561 agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag ccgacgctta
    19621 atagccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg ttcgtcgccg
    19681 tacgcgtggg taaaatcatg atgcatacgc aagaaatact cttcgttcag gcgctcaaga
    19741 cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg ctcgcgcagg
    19801 cattccggca ggtgattcga accgatcatc cggctttcgt cttccagcac ccacaggcgc
    19861 aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc aaaactcgcc
    19921 tggctaagtt gtggttgtaa ggtgcgacca aacgccgaac cgcgatgacg cgctaaccct
    19981 tccagatcaa caccgttcgg ctgttgctgc actaacagcg ttttaccgct gccggtacaa
    20041 ccgccaatca gcactatcgg tttttgtgcc agttcaatag tcgcctgaat cgcggcttgg
    20101 cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgctgc atacaaccag
    20161 ctttgcacaa tatgtgagcg ctgaccgcca cgggcgcagc agagaatacc ttgcggattt
    20221 tgcaggcacg ctgcccgcca ggcgtccatg cgctgctgac gaatttcacc cgccaccagt
    20281 ttatgcccca gcgccagcgc cgcgtctgag ccttgctgtt tataacaagt gccaacggcg
    20341 gcgcgttcat cgttattcat taacggcagg ttgatagcgg cgggcattgc gccttgctca
    20401 aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag ggcacgatag
    20461 tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag cttgcgcgct
    20521 attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtgggttg ccgaaaattt
    20581 ctaaaaatcc gctgatttcc ggcctgcgct gggtaaacaa ggtcacaata tcttctaccg
    20641 ctttgccgct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg cgcatcgttg
    20701 ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt gattttggca
    20761 aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata tcgggaacaa
    20821 taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtt cgggcgctgt
    20881 cttcaatatt gaccgccgga aagcgccgca actgcgcttc tgttagcggc tcttcaacgt
    20941 tcgccagcgg atgatccgcc gccatgacaa agcgccattg caccgatcct aagggatcaa
    21001 gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc gaaaaacctt
    21061 cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga aactgggtaa
    21121 aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg ttatagagca
    21181 ggttgttgat gacaatattc acctagcgtt ccacgccatc attcacctgt tgcagctcgc
    21241 tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga tgctcgccag
    21301 ccgctgtcaa cgtcacgctg cgagtcgtgc ggaaaaacag cgccactccg gtattctctt
    21361 ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt tctgccgctt
    21421 ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct ggatcgaaca
    21481 tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct ctgatttatc
    21541 agtattttta catgataacc ctgttcaatt tgtggactaa atctagtttt ggaaaaatat
    21601 tccaactttt gtattgatgt tgttctctta aggttttaga t
//
LOCUS       DAJTXQ010000068        21536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000068 DAJTXQ010000000
VERSION     DAJTXQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.068"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1365
                     /gene="espL1"
                     /locus_tag="OO515_002412"
                     /pseudo
     CDS             1..1365
                     /gene="espL1"
                     /locus_tag="OO515_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1537..1644
                     /locus_tag="OO515_002413"
     CDS             1537..1644
                     /locus_tag="OO515_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576330.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1697..2455)
                     /locus_tag="OO515_002414"
     CDS             complement(1697..2455)
                     /locus_tag="OO515_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6576331.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2742..3671
                     /gene="pfkB"
                     /locus_tag="OO515_002415"
     CDS             2742..3671
                     /gene="pfkB"
                     /locus_tag="OO515_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6576332.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3772..4062
                     /gene="ghoS"
                     /locus_tag="OO515_002416"
     CDS             3772..4062
                     /gene="ghoS"
                     /locus_tag="OO515_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6576333.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4168..5028
                     /locus_tag="OO515_002417"
     CDS             4168..5028
                     /locus_tag="OO515_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6576334.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5069..5605)
                     /locus_tag="OO515_002418"
     CDS             complement(5069..5605)
                     /locus_tag="OO515_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6576335.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5752..6420
                     /gene="hxpB"
                     /locus_tag="OO515_002419"
     CDS             5752..6420
                     /gene="hxpB"
                     /locus_tag="OO515_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6576336.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6583..7173
                     /locus_tag="OO515_002420"
     CDS             6583..7173
                     /locus_tag="OO515_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6576337.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7306..8697
                     /gene="tcyP"
                     /locus_tag="OO515_002421"
     CDS             7306..8697
                     /gene="tcyP"
                     /locus_tag="OO515_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6576338.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8701..9517)
                     /locus_tag="OO515_002422"
                     /pseudo
     CDS             complement(8701..9517)
                     /locus_tag="OO515_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OO515_002423"
     CDS             complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OO515_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6576339.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10240..12501
                     /gene="katE"
                     /locus_tag="OO515_002424"
     CDS             10240..12501
                     /gene="katE"
                     /locus_tag="OO515_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6576340.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OO515_002425"
     CDS             complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OO515_002425"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6576341.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OO515_002426"
                     /gene_synonym="chbF"
     CDS             complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OO515_002426"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6576342.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OO515_002427"
     CDS             complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OO515_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6576343.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OO515_002428"
     CDS             complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OO515_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6576344.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OO515_002429"
     CDS             complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OO515_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6576345.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OO515_002430"
     CDS             complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OO515_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6576346.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OO515_002431"
     CDS             complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OO515_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6576347.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18840..19667
                     /gene="nadE"
                     /locus_tag="OO515_002432"
     CDS             18840..19667
                     /gene="nadE"
                     /locus_tag="OO515_002432"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6576348.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19897..20784
                     /gene="cho"
                     /locus_tag="OO515_002433"
     CDS             19897..20784
                     /gene="cho"
                     /locus_tag="OO515_002433"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6576349.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OO515_002434"
     CDS             complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OO515_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6576350.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5735 a         5311 c         4935 g         5555 t
ORIGIN      
        1 cccttgattc attgagaaat ggaaaaggca aagtcaacct gattaaacac tactcctccg
       61 ttgaatccat acagcagcat gtccccttag tccgggacgc ggagttcaga gcattacttc
      121 gccatcctcc tgcagggagt cgcgttatcg cgagtaagga ttttggcttc gctttagata
      181 ttttcttttg tcgaatgatg gcaaacaatg tcagtcatat gtccgcgatt ttatatatag
      241 acaatcatac tttgtcagta aggctacgaa taaagcagtc agtgtatgga caattaaatt
      301 atgttgtgtc tgtttacgac ccgaacgata ccaacgttgc cgtcagaggc acccacaggg
      361 cagcacgggg ctttctctcg cttgataagt tcatcagttc agctcccgat gctcagacct
      421 gggctgatag gtatgttcgc aactgtgcaa ttgctattct tcctctatta cctgagggag
      481 ttccagttgc tattttagcg ggtatcacga cacgaatgcc atttgcccct atacatccat
      541 cggcaatgtt gttaataatg gccacaggcc agactcaata gcttattaca ctattcaaac
      601 agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag aatagcgttg
      661 gtacacctgc tttatttctg gctatgatga acggacaaac tgacaacgta aaaatattta
      721 tgcaagaaat tcagtcactg gtatacaatc atatcattca tgaagataat ctggttaaat
      781 tactgcaaac taaaagtgct aacgaaacac ctggactcta tatctccatg ttgtatggat
      841 tcgatgaaat aatcgatatc tttctgaatg cattaaccac tccaatagca caagaacttt
      901 taaacaaaaa aaatggtgat gagtatttta gccatgaaaa tacatgatgg tgagccagga
      961 ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct ctctaaaatt
     1021 aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt attaaaagga
     1081 gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa tgaagacgtc
     1141 gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt tagtcaacat
     1201 cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt tcatatagcc
     1261 attcatcata agcattataa aactgtagaa acatattatg ctgctataaa tgtcatcagc
     1321 caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc tatttacgat
     1381 atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac catgtgcaca
     1441 acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctatccctt ttgttacact
     1501 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
     1561 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
     1621 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac
     1681 ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cgatcggtat
     1741 gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc
     1801 caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt gtatcttccg
     1861 cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
     1921 ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac
     1981 gcacacctgg gccgaattca aagcggaaac tgtgtaccgg gccattcaaa aactggcgac
     2041 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
     2101 ttgcctgacc aaataaatag tcataatcag ttaaattaaa acggctacgc ccgcccgccg
     2161 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
     2221 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
     2281 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
     2341 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
     2401 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     2461 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     2521 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     2581 ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt atatatattt
     2641 atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt cactttccgc
     2701 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
     2761 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
     2821 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
     2881 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
     2941 aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
     3001 actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag tatcgttttg
     3061 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
     3121 aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt gtgaagctgg
     3181 aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc atcgtcgaca
     3241 gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg gttaagccta
     3301 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac gatgtccgca
     3361 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
     3421 cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga
     3481 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
     3541 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
     3601 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
     3661 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     3721 cgcgtttctc cgactttgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     3781 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     3841 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     3901 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     3961 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     4021 accatcacga cttgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     4081 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     4141 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     4201 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     4261 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     4321 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     4381 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     4441 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     4501 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     4561 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     4621 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     4681 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac tgcagccttc tctgttgcac
     4741 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     4801 gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag
     4861 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     4921 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag
     4981 catttggtta ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg
     5041 ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     5101 aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc aggaaaatag
     5161 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     5221 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     5281 tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     5341 agaacataac gttcacgcca ccattaaaat taggggtcgg ggagttatac cagaacagat
     5401 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga
     5461 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     5521 atgtggagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     5581 tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     5641 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     5701 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     5761 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     5821 ttacgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt
     5881 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     5941 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     6001 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta
     6061 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     6121 aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     6181 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     6241 gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca
     6301 gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     6361 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     6421 tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac
     6481 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     6541 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     6601 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     6661 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     6721 atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc
     6781 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     6841 accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg
     6901 gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     6961 ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa
     7021 ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     7081 cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca gatccagttt
     7141 catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct ttctggaata
     7201 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     7261 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     7321 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa
     7381 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     7441 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     7501 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     7561 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     7621 tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg
     7681 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     7741 cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag cgttccgcag
     7801 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg
     7861 tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg
     7921 aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct acaaagctgg
     7981 gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg
     8041 accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc
     8101 gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat
     8161 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     8221 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     8281 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     8341 tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac
     8401 ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc
     8461 gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc
     8521 ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt
     8581 acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag
     8641 caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc
     8701 ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta aatgttccat
     8761 cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa tgtccaactt
     8821 atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa cctcataccc
     8881 gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca caccacttcc
     8941 cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg atattatcga
     9001 aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc ccggcaggat
     9061 gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac ctatttttta
     9121 tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc atcccaacaa
     9181 aattctcatt aatatctctt atccgaaact cactgccata aagattatta attatcactc
     9241 tatatttaac attgtcctgc agatacgtat caaaactaag tgctggagct cctggctttg
     9301 aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc gactctgcta
     9361 tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc atggtagact
     9421 caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca ccataattac
     9481 tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa ttattatctc
     9541 atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga tgaactatca
     9601 aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa caattaaaga
     9661 aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt acaggtggtt
     9721 tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg caaaaccggg
     9781 aagtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt gcccaacgtt
     9841 gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc atcagtacaa
     9901 acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc tttatcgcat
     9961 gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg ttttgctggc
    10021 ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga aaaactgtag
    10081 tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca ccgtttccag
    10141 aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt ttgaccaaaa
    10201 cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca taacgaaaag
    10261 aacctacatc agcaccagtc accactacac gattccagcg aagcgaaacc ggggatggac
    10321 tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc gccaggtgca
    10381 caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa acttaattct
    10441 ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc
    10501 atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct gctggaagat
    10561 tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga acgtattgtt
    10621 catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt aagcgatatc
    10681 accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt taggttctct
    10741 accgttcagg gtggcgctgg ctctgcagat accgtgcgtg atatccgtgg ttttgccacc
    10801 aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc aatcttcttt
    10861 atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga accgcactgg
    10921 gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc tctgcaacct
    10981 gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc
    11041 accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg gaaggcaacg
    11101 tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg ggatgaagca
    11161 caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga agccattgaa
    11221 gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga agatgaattc
    11281 aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact ggtgcccgtt
    11341 cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc cgaaaacgaa
    11401 caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa cgatccgctg
    11461 ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg tgggccgaat
    11521 ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca gcgtgatggc
    11581 atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc gatcaacgat
    11641 aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc atatcaggaa
    11701 cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata ttattcccat
    11761 ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt cgatggtttc
    11821 agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg
    11881 gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat cgaactgact
    11941 gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc
    12001 ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc gattttactt
    12061 aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa ggccaaaggc
    12121 gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg
    12181 ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc
    12241 ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta cctgatggaa
    12301 gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt taaagcaaca
    12361 atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc tgacggtagt
    12421 tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg cattcctaag
    12481 attgacaaaa ttcctgcctg atgggaggcg gcgcaattgc gccgcctcaa atgatttaca
    12541 tagtgcgctt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caaaactgtg
    12601 caaattcaat atattgcagg aaacacgtag gcctgataag cgaagcgcat caggcagttt
    12661 tgcgtttgtc agcagtctca agcggcgcag ttacgccgcc tttgtaggaa ttaatcgccg
    12721 gatgcaaggt tcacgccgca tctggcaaac atcctcactt acacatcccg ataactcccc
    12781 aaccgataac cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa cacatccagc
    12841 tccgttaagc gtgggaaaca gtaagcactc tgacggatag tattatcgat aaacgccgga
    12901 tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag cacttgcagg
    12961 aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg agaactgcgc
    13021 agattagccg gtaaatcacc agagttagat agcggctgac ggtcgatgcg caacgcaatc
    13081 ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat atgcacatga
    13141 tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga
    13201 ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag
    13261 atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttgccc
    13321 atagtaagga caaagtacat ccctatggcc agactcggtt catcacgact caactgcacc
    13381 gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat cccattgcga
    13441 caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca
    13501 atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag
    13561 tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca
    13621 gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat
    13681 tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt gtgaatcagc
    13741 cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc
    13801 agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg cccatgatgc
    13861 gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat cacttcgcac
    13921 gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcaac
    13981 tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt
    14041 gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg cttgaagtaa
    14101 taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc gctaaatggc
    14161 agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac accatcaagc
    14221 aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg
    14281 aggccgaaca aatcgataga taaatcatcg ctgtctttca gcatcagaac atcgtgaata
    14341 aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga
    14401 cgataaacgg cttcagtaac cattcccgcc gggttagtga agttaatcac ccatgcattc
    14461 ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct
    14521 ttaaacagac cgcccgcgcc gttggtttcc tggccaagat aaccatgact taatggaata
    14581 cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt aacgaaatca
    14641 gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg
    14701 ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt accatcttcg
    14761 acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc
    14821 agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc
    14881 ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc ccggacataa
    14941 ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt
    15001 cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa
    15061 taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg gaatagttgg
    15121 tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc tgcattggtg
    15181 ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg
    15241 tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc ttttctaccg
    15301 tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac
    15361 ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat
    15421 tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct
    15481 gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac
    15541 tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga tggtgcgaac
    15601 ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct
    15661 ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga tgcagcccgc
    15721 tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct
    15781 gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat
    15841 gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac
    15901 gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc
    15961 gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac
    16021 tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt cagtgccgca
    16081 tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc
    16141 tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca tcgttcctct
    16201 ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga
    16261 tatcttcttc gcgctcttct ttatcaatcg cgttttgtgc tttgttagcc accacaacaa
    16321 agggcagata aattaacgtt gcgataccaa ggttgaagag tgcaaccagc aatgcggcga
    16381 cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac ggtgcaatat
    16441 tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc gccagaatcg
    16501 gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca
    16561 gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc acctgacgat
    16621 aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc
    16681 ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg
    16741 ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc
    16801 acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga
    16861 accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc aacgatgcca
    16921 gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg
    16981 cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat gccgagaagg
    17041 aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg cggcggacaa
    17101 taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg atattcgctc
    17161 cacctaacca gtttgcgcca accacatagg cctcaccgac actatatggg gtgacggtca
    17221 taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc tcttctgcca
    17281 gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata
    17341 cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggcg tcgaggcgaa
    17401 tacctaagga ataaaaaaac gccccctccc caaagcttag aaaaacgttg ttaattaata
    17461 caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat
    17521 taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg
    17581 caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg
    17641 atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt
    17701 taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg
    17761 agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag
    17821 caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt
    17881 ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg
    17941 ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca
    18001 taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct
    18061 tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata
    18121 cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt
    18181 ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc
    18241 caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat
    18301 tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg
    18361 acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc
    18421 aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta
    18481 cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc
    18541 tgtacaaact ggtctttagt acgatcataa gccgtacaac ccgccagcat ggttaatacc
    18601 gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag
    18661 cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgcgaac gaaacggctc
    18721 cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg
    18781 tttcagtggc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa
    18841 tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag
    18901 aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat ccgttcatta
    18961 aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc
    19021 agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa tttattgccg
    19081 tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta
    19141 ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc agcgaacagg
    19201 cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg
    19261 agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg
    19321 atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc ggtacagaca
    19381 ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattaggtt
    19441 gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat cgcccttctc
    19501 tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga
    19561 aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata
    19621 aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata
    19681 atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg
    19741 acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg acaggcgtag
    19801 cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc tccccgcctg
    19861 ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg
    19921 cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt
    19981 aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg
    20041 ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg
    20101 gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgtac cgcaggcgaa
    20161 atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa
    20221 cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg
    20281 gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat
    20341 aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact ttgttatggc
    20401 ctgctgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt
    20461 tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag
    20521 tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa
    20581 cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt
    20641 aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt
    20701 tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg
    20761 gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa
    20821 gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcaatttat
    20881 cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag
    20941 taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt
    21001 tgaaatccat cgacatctgc ccttccgtca gtttcgcttt taccacctgg tccgctgcaa
    21061 aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggagca
    21121 tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa gaaaactccc
    21181 cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga
    21241 aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc gacattttac
    21301 gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt
    21361 aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt
    21421 acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt
    21481 agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc agttgc
//
LOCUS       DAJTXQ010000069        21401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000069 DAJTXQ010000000
VERSION     DAJTXQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.069"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="OO515_002435"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="OO515_002435"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6576351.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="OO515_002436"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="OO515_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6576352.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="OO515_002437"
     CDS             complement(2378..2992)
                     /locus_tag="OO515_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6576353.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="OO515_002438"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="OO515_002438"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6576354.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVELLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="OO515_002439"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="OO515_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6576355.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="OO515_002440"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="OO515_002440"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6576356.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="OO515_002441"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="OO515_002441"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6576357.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="OO515_002442"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="OO515_002442"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6576358.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="OO515_002443"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="OO515_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6576359.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="OO515_002444"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="OO515_002444"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6576360.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="OO515_002445"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="OO515_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6576361.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="OO515_002446"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="OO515_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6576362.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="OO515_002447"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="OO515_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6576363.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="OO515_002448"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="OO515_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6576364.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="OO515_002449"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="OO515_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6576365.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="OO515_002450"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="OO515_002450"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6576366.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="OO515_002451"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="OO515_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6576367.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="OO515_002452"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="OO515_002452"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6576368.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="OO515_002453"
     CDS             19125..19937
                     /locus_tag="OO515_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6576369.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21401
                     /gene="hcp"
                     /locus_tag="OO515_002454"
                     /pseudo
     CDS             20331..21401
                     /gene="hcp"
                     /locus_tag="OO515_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5251 a         5720 c         5371 g         5059 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagaggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcagttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcaggt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt t
//
LOCUS       DAJTXQ010000070        20993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000070 DAJTXQ010000000
VERSION     DAJTXQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.070"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..160)
                     /locus_tag="OO515_002455"
     CDS             complement(<1..160)
                     /locus_tag="OO515_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576370.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFG"
     gene            332..1552
                     /gene="coaBC"
                     /locus_tag="OO515_002456"
     CDS             332..1552
                     /gene="coaBC"
                     /locus_tag="OO515_002456"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6576371.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1530..1988
                     /gene="dut"
                     /locus_tag="OO515_002457"
     CDS             1530..1988
                     /gene="dut"
                     /locus_tag="OO515_002457"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6576372.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2095..2691
                     /gene="slmA"
                     /locus_tag="OO515_002458"
     CDS             2095..2691
                     /gene="slmA"
                     /locus_tag="OO515_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6576373.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2728..3369)
                     /gene="pyrE"
                     /locus_tag="OO515_002459"
     CDS             complement(2728..3369)
                     /gene="pyrE"
                     /locus_tag="OO515_002459"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6576374.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3435..4151)
                     /gene="rph"
                     /locus_tag="OO515_002460"
     CDS             complement(3435..4151)
                     /gene="rph"
                     /locus_tag="OO515_002460"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6576375.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4278..5141
                     /locus_tag="OO515_002461"
     CDS             4278..5141
                     /locus_tag="OO515_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6576376.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5363..6187
                     /gene="dinD"
                     /locus_tag="OO515_002462"
     CDS             5363..6187
                     /gene="dinD"
                     /locus_tag="OO515_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6576377.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6479..7096
                     /locus_tag="OO515_002463"
     CDS             6479..7096
                     /locus_tag="OO515_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6576378.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7093..8775)
                     /gene="ligB"
                     /locus_tag="OO515_002464"
     CDS             complement(7093..8775)
                     /gene="ligB"
                     /locus_tag="OO515_002464"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6576379.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9033..9656
                     /gene="gmk"
                     /locus_tag="OO515_002465"
     CDS             9033..9656
                     /gene="gmk"
                     /locus_tag="OO515_002465"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6576380.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9711..9986
                     /gene="rpoZ"
                     /locus_tag="OO515_002466"
     CDS             9711..9986
                     /gene="rpoZ"
                     /locus_tag="OO515_002466"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6576381.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10005..12113
                     /gene="spoT"
                     /locus_tag="OO515_002467"
     CDS             10005..12113
                     /gene="spoT"
                     /locus_tag="OO515_002467"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6576382.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12120..12809
                     /gene="trmH"
                     /locus_tag="OO515_002468"
     CDS             12120..12809
                     /gene="trmH"
                     /locus_tag="OO515_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6576383.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12815..14896
                     /gene="recG"
                     /locus_tag="OO515_002469"
     CDS             12815..14896
                     /gene="recG"
                     /locus_tag="OO515_002469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6576384.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15065..16270)
                     /gene="gltS"
                     /locus_tag="OO515_002470"
     CDS             complement(15065..16270)
                     /gene="gltS"
                     /locus_tag="OO515_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6576385.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16550..17941
                     /gene="xanP"
                     /locus_tag="OO515_002471"
     CDS             16550..17941
                     /gene="xanP"
                     /locus_tag="OO515_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6576386.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18062..19771
                     /locus_tag="OO515_002472"
     CDS             18062..19771
                     /locus_tag="OO515_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6576387.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19823..>20993)
                     /gene="yicI"
                     /locus_tag="OO515_002473"
     CDS             complement(19823..>20993)
                     /gene="yicI"
                     /locus_tag="OO515_002473"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6576388.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5077 a         5711 c         5384 g         4821 t
ORIGIN      
        1 agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg cgcgttccgg
       61 tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca aacttcagca
      121 ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc tttgtggtgc
      181 tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc tggaaagcgc
      241 ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata aaatcgccaa
      301 cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa aaatcgttct
      361 cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc gtttgcgcga
      421 tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta tcaccccact
      481 tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc cggcagccga
      541 agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc tcgcccctgc
      601 cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg tatcgacgat
      661 ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc agatgtaccg
      721 tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc tcatctgggg
      781 gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc tcgatccgtt
      841 aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga aacatctgaa
      901 cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt atatctctaa
      961 tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc gtggcgcgaa
     1021 cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta aacgtgttga
     1081 tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc agcaaaatat
     1141 ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc cagagaaaat
     1201 caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa accccgatat
     1261 cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat ttgccgccga
     1321 aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc ttgatctgat
     1381 ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca acgcattaca
     1441 ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc tccttggcca
     1501 attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt aagattctgg
     1561 acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc tctgccggac
     1621 ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac tctacgctgg
     1681 ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg atgctgccgc
     1741 gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga ttgatcgatt
     1801 ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac agcttcacca
     1861 ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag gctgaattta
     1921 atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt cactctggtc
     1981 gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat ttcgtttgcg
     2041 gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg taacatggca
     2101 gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct ggcgctgatg
     2161 ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc ctctgtcggc
     2221 gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt cgatagtctg
     2281 attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa agatgagaaa
     2341 gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg tgagcgtaat
     2401 cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga tcgcctgcaa
     2461 gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt attgcgtgaa
     2521 aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc aagccagatc
     2581 ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa ataccgcccg
     2641 acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta atatgacgcc
     2701 ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat aggccttaac
     2761 cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca ggtctttcag
     2821 ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa tcgccgaaat
     2881 ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa gcgtcgcgcc
     2941 attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga tcacatcatc
     3001 taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt caccgtggtt
     3061 ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct ccgccagcgc
     3121 cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca gcagatcgaa
     3181 ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg ccaggtcgcg
     3241 cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg atttcagcgt
     3301 aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact ggcgctgata
     3361 tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag gcgactcatc
     3421 agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat ggattcgatt
     3481 ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg ttcgccttct
     3541 gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt catgtcggtc
     3601 tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt cacaatcccg
     3661 acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc gttttccacc
     3721 agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc ggtacgagtg
     3781 ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc cagcgctttc
     3841 aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat tgtgcgtcca
     3901 ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg cggcagcata
     3961 ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg cacgccttct
     4021 tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac cgagccttct
     4081 gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt gctacggcct
     4141 gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt atacggactt
     4201 ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca cctctccttt
     4261 tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt gaaatcaagg
     4321 gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat ctggaaactt
     4381 actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag cgtattcgtt
     4441 ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat gttagcgcgc
     4501 aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc gcgaactggg
     4561 taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc tggccgggcg
     4621 taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc gcggcgttgg
     4681 atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg ctgaaagcgt
     4741 tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc gcccatatgc
     4801 cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat gctcaagtgc
     4861 aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga attgatgttg
     4921 ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt ctgaagaaaa
     4981 aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc gagtcgaaca
     5041 ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag ctgaaagtgt
     5101 tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca ctaattctta
     5161 cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct tccccgcaac
     5221 attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc gcagcgcagt
     5281 gagaacgtga gatactcaca actgtatata aatacagtta tagacttact ttctttgcaa
     5341 tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag aaatacggca
     5401 ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt tactggatta
     5461 tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt gcgaagccag
     5521 caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat taggttcagg
     5581 ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc tggtagtaca
     5641 aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg ctatccagac
     5701 ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag acgaaaagcg
     5761 tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg cagcgcagca
     5821 agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt accgggggct
     5881 gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa agagtcagaa
     5941 gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag ctacccaaac
     6001 agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata ccacacactt
     6061 tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca tgcctgagga
     6121 gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa ttacagagaa
     6181 gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg taagccaaag
     6241 tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc cctcaatttt
     6301 aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca tctacactac
     6361 agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt aaaaaatctc
     6421 aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg agagaaccat
     6481 gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg gggcgctggc
     6541 ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg ccaccgcaat
     6601 tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt gggtcaaaca
     6661 cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg ttgctcctgt
     6721 aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg tggtcttttc
     6781 tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg ccgttgtcgc
     6841 ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta aacgcatccc
     6901 actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg tgctgtacat
     6961 cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc tggtattcgg
     7021 cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct tttactacag
     7081 ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc aactgcccag
     7141 cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc cagatccagt
     7201 ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc aggaccgttc
     7261 atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca cccagcgagt
     7321 aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt tacttttcgc
     7381 gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa agatatgctc
     7441 aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat ccagcccaag
     7501 aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac aaacatcaga
     7561 agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt ttgtacgttc
     7621 tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa ggctgacgag
     7681 gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac caatattcac
     7741 ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca ccgatatttt
     7801 accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag ctacaggttg
     7861 atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg attcgggttc
     7921 cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt tccaccactc
     7981 attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc tctgcgttag
     8041 agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg gtccatccgg
     8101 ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct ggcgcatcat
     8161 caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga tatgcccctc
     8221 gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc taacggtttg
     8281 cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca ggccattacc
     8341 gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg caacgccatc
     8401 aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta atgcgttttt
     8461 atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac catttaaagg
     8521 cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg taagacgggc
     8581 gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc agtagtcatc
     8641 gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc tggctggcga
     8701 ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta ttaatatcgc
     8761 catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac aacgtgatga
     8821 aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa tcgtctttat
     8881 tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac tttgcgcaaa
     8941 aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc gtatgtaggc
     9001 tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt tatattgttt
     9061 ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa acccaaccgt
     9121 tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct ggtgaagtcc
     9181 acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt agcagagatg
     9241 cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt gaggccattg
     9301 agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag ggcgcgcagc
     9361 aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg tccaaaattg
     9421 aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt gcaaagcgta
     9481 tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg attgtgaatg
     9541 atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa cgtctgcgca
     9601 tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca gactgaacct
     9661 gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt atggcacgcg
     9721 taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta ctggtcgccg
     9781 cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg gaagaaaacg
     9841 ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac aaccagatcc
     9901 tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta caagccgtta
     9961 ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat ctgtttgaaa
    10021 gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt ctgcggcagg
    10081 cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt gaaccctaca
    10141 tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac tatgaaacgc
    10201 tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac caggatatgg
    10261 aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa cttgataaac
    10321 tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg attatggcga
    10381 tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac aacatgcgca
    10441 cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact ctcgaaattt
    10501 acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc gaagagctgg
    10561 gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg aaagccgcgc
    10621 gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg cgtttgcagg
    10681 aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg atttactgca
    10741 aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct ttccgcgtga
    10801 tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc ctgtacaagc
    10861 cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc tatcagtctt
    10921 tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc cgtactgaag
    10981 atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa gagcacggcg
    11041 aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcttg ctggagctgc
    11101 aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat ctcttcccgg
    11161 atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc ggtgcaacgc
    11221 ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg ggcgcacgcg
    11281 ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc gttgaaatca
    11341 ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt agctcaaaag
    11401 cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct gtaagcctgg
    11461 gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa atcccgcagg
    11521 aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat ctgctggcag
    11581 aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa catggggacg
    11641 cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt gccgatggcg
    11701 tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt atcgcccacg
    11761 tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc cgtggctacc
    11821 agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg caggagttca
    11881 tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac ctgacggcgg
    11941 caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa gatggtcgcg
    12001 tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg aatatcatgc
    12061 gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat taatgtttta
    12121 tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag cctgatctga
    12181 ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt cgtaccgcag
    12241 atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg cgcaccatgg
    12301 cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc accattggcg
    12361 atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat ctttctgata
    12421 acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg atgggacagg
    12481 agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc atcattccga
    12541 tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt ctttacgaag
    12601 cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg ttgccggaag
    12661 cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa gtcgcaaaac
    12721 gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct gatgccgact
    12781 ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt tagatgctgt
    12841 cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg cgaaaatcaa
    12901 cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag atcgcaccca
    12961 tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag gcgaagtgct
    13021 gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca gcgacggttc
    13081 cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata gcctggcgac
    13141 gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg cggagatgat
    13201 ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg aaacgctcac
    13261 gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat taaccgacca
    13321 ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg aactgtcaca
    13381 aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac cgacgctaca
    13441 gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc tggaagaact
    13501 gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt ttcatgccca
    13561 gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac cgttcaagcc
    13621 aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc tggatgtgcc
    13681 gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg ccgccctcgc
    13741 cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa ccgaattact
    13801 cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta tcgaagtggg
    13861 ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag ccatcgccag
    13921 cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg tgcagtttaa
    13981 cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc agcgtctggc
    14041 gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca tgaccgccac
    14101 gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct cggtgataga
    14161 tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata ctcgccgtac
    14221 cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg cgtactgggt
    14281 ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag ctacctggga
    14341 agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc ggatgaaacc
    14401 agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc tgctggttgc
    14461 cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga ttatcgaaaa
    14521 ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag gtcgtggcgc
    14581 ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg cgcaaattcg
    14641 cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag atctggagat
    14701 tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat ttaaagtggc
    14761 ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac gccatattca
    14821 cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg agacggaacg
    14881 ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta ccctaccttc
    14941 ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat acgcaggctt
    15001 gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac cgccgcttca
    15061 tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc gcattgacga
    15121 tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc gacgggccaa
    15181 agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg aggccaaaac
    15241 cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg cgccaggtaa
    15301 cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc agcatcggca
    15361 gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc aggaacaacg
    15421 acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa aagcccatta
    15481 ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag gtcggcagtt
    15541 caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg cagatagcaa
    15601 tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca tccggctttt
    15661 caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg gagtgtttca
    15721 ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg aacgttgcac
    15781 aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac aatttactcc
    15841 acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac agccccatca
    15901 gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc atcaccaaca
    15961 gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc aaactggcaa
    16021 tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg cgcagggaca
    16081 tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc agcgccacca
    16141 acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag acggaatgga
    16201 ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct aaagtatcga
    16261 gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc atacttcatc
    16321 gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct gggtcaaatt
    16381 ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa aagttatata
    16441 aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg tttgcttttc
    16501 tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga tgtctgtttc
    16561 caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg aactgattta
    16621 ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc agcatctgct
    16681 ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc tgggtttacc
    16741 ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg tggcatcgat
    16801 tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc agggcaccag
    16861 cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg gtggtgctga
    16921 tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt gcaccgagat
    16981 ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg tttctggcgt
    17041 tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca ttggcggcgg
    17101 ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc tggcaggcgt
    17161 ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac gcgtggcctc
    17221 actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca tgttgccaga
    17281 aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct attacggtct
    17341 tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca cttcgctgga
    17401 aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt ccggtccgct
    17461 gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt ttgtttcggc
    17521 ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga tccagttgac
    17581 cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg tgctgggtct
    17641 gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg gcggcgcaac
    17701 gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc gtgagccgct
    17761 gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg gcgtgtctca
    17821 gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct cctccgggat
    17881 cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag aaaaacagta
    17941 attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac cattcccgcc
    18001 ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg gatggaagac
    18061 catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag tggcgatcgc
    18121 tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg catgggtttc
    18181 cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt ccgcgccatc
    18241 tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga ccctggtggc
    18301 caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc atgtcgatac
    18361 catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc taccgttcaa
    18421 agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg aatggaaact
    18481 gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg gtaaagtgct
    18541 gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg atgttcctgc
    18601 caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga ctaacctggg
    18661 tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg acggcagctg
    18721 gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat cgctaaccga
    18781 cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg aagtgatcga
    18841 tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct tgcgcaacat
    18901 gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga acgctagcga
    18961 gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat tttctccgca
    19021 gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca gaacgtcagg
    19081 gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg ccggactgga
    19141 atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg gttggttaaa
    19201 cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata tcgaccctga
    19261 cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc tggttaccga
    19321 tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg cgacgtttaa
    19381 tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca cggtgaatat
    19441 cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca gtgtttcaca
    19501 aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg tgaatatttt
    19561 gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc agcttaccgc
    19621 cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc aattgcatgc
    19681 cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg tttccagcgg
    19741 tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta cgactcagac
    19801 gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct tgaggcgtaa
    19861 ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt actttcacaa
    19921 tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca gtaatcgtgt
    19981 taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg ggcacttcac
    20041 atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct tcgtgccacg
    20101 cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca cgcacataaa
    20161 cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg agctcattgt
    20221 tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg cccgcctcag
    20281 tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca aggtaatcac
    20341 aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta ccccgcgctt
    20401 tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt tgcgtgaaga
    20461 agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa gatttgctgc
    20521 catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg taaacgtgtg
    20581 ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa cctgaaaggc
    20641 caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg taacaatcgc
    20701 caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca aacaagacag
    20761 cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg tagatgtacg
    20821 cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca tcagttggga
    20881 tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga cctttcagtt
    20941 tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc aga
//
LOCUS       DAJTXQ010000071        20855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000071 DAJTXQ010000000
VERSION     DAJTXQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.071"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..200)
                     /locus_tag="OO515_002474"
     CDS             complement(<1..200)
                     /locus_tag="OO515_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6576389.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALA"
     gene            complement(197..1758)
                     /gene="casA"
                     /locus_tag="OO515_002475"
                     /pseudo
     CDS             complement(197..1758)
                     /gene="casA"
                     /locus_tag="OO515_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1856..4555)
                     /locus_tag="OO515_002476"
     CDS             complement(1856..4555)
                     /locus_tag="OO515_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCR6576390.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4750..4902)
                     /locus_tag="OO515_002477"
     CDS             complement(4750..4902)
                     /locus_tag="OO515_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6576391.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5167..5901)
                     /gene="cysH"
                     /locus_tag="OO515_002478"
     CDS             complement(5167..5901)
                     /gene="cysH"
                     /locus_tag="OO515_002478"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6576392.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5976..7688)
                     /gene="cysI"
                     /locus_tag="OO515_002479"
     CDS             complement(5976..7688)
                     /gene="cysI"
                     /locus_tag="OO515_002479"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6576393.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7688..9487)
                     /gene="cysJ"
                     /locus_tag="OO515_002480"
     CDS             complement(7688..9487)
                     /gene="cysJ"
                     /locus_tag="OO515_002480"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6576394.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9806..10168
                     /gene="queD"
                     /locus_tag="OO515_002481"
     CDS             9806..10168
                     /gene="queD"
                     /locus_tag="OO515_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6576395.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10246..11517
                     /locus_tag="OO515_002482"
     CDS             10246..11517
                     /locus_tag="OO515_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6576396.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11508..11768
                     /locus_tag="OO515_002483"
     CDS             11508..11768
                     /locus_tag="OO515_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6576397.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11785..12360
                     /locus_tag="OO515_002484"
     CDS             11785..12360
                     /locus_tag="OO515_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6576398.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12508..13368)
                     /locus_tag="OO515_002485"
     CDS             complement(12508..13368)
                     /locus_tag="OO515_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6576399.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13365..14144)
                     /locus_tag="OO515_002486"
     CDS             complement(13365..14144)
                     /locus_tag="OO515_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6576400.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14122..15531)
                     /locus_tag="OO515_002487"
     CDS             complement(14122..15531)
                     /locus_tag="OO515_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6576401.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15553..17007)
                     /locus_tag="OO515_002488"
     CDS             complement(15553..17007)
                     /locus_tag="OO515_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6576402.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17077..17862)
                     /locus_tag="OO515_002489"
     CDS             complement(17077..17862)
                     /locus_tag="OO515_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6576403.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVNGGYLVR"
     gene            18180..19457
                     /locus_tag="OO515_002490"
     CDS             18180..19457
                     /locus_tag="OO515_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6576404.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWIFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19484..20855
                     /locus_tag="OO515_002491"
                     /pseudo
     CDS             19484..20855
                     /locus_tag="OO515_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         5000 a         5544 c         5137 g         5174 t
ORIGIN      
        1 gccagtgcgg tgatacgcca ctcaggttca tcctgccact ttatttgtct gttcttcaga
       61 aacaggcgaa atccatccgt cagacaaaca tcattaaccg tcgtactacg ccgcagactg
      121 gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt tgcgcagcgt ggctttgtgc
      181 tcctctttta caatactcat tcagcagcct cctgcttttt ctccctggcg gctttagcac
      241 tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc ggtcatgatg cgttccaaat
      301 caacgggctc ataaggattg gtgaatacac gctcgtcaaa atcctgacgc gcgaataacc
      361 aaatttcctt attccatttg ctgagtaatt catccgcatc ctgaccttct tcaatttggc
      421 gcactaacct caggaagcga tgctgagttt tgttccagaa gtcgatatcc acaaaactaa
      481 aatcacccct tgcacctttt ggatcggaga accatgcttc tttcaatgca ctccgtaaca
      541 gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag tttcggtatc tggccttctt
      601 tttattgagc agcagcggga aatgatgttc gtaccaacag cgcgctttca tgttgtcgaa
      661 atcatagcca aatccccaca ggcccacttt tgcctgcttg agactgctgg cattaaagag
      721 tttcaccacc agcgcgggaa gttccgtatt gttttctgac ttacccgttt cgataaggcc
      781 taaccagtcg cgccagatta aaccgcccgg ttgtggtttg accgagtaaa actcgccgcc
      841 ctctttaagt agtacacggt aaggcgttaa gggatgctgc cacatggcat agttagcgcc
      901 gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc tgctcaccgc aaatatcgca
      961 gttgccgact gtggtggtat taaaatcaat acgaatacgg cgaggcattc cccagtacgc
     1021 ctggagttta ttgacctgct catcggttac caccgcaccg gccagttcgc tgatgcgcgt
     1081 cgggccaagc caggggaaaa ccagatcgtc aaattttttg ggtagcggta aatcggcttc
     1141 atcctgcggc atgacattga tccacagttt gcgccacaag ggagtttgtt gattaccctg
     1201 atattcctgt aattcaatca gagtggtcat cggcccaccg ccgcgtaaac cggtgcggta
     1261 gcctttgcca cctgacggcg cattcaactg tagggagaac agagctaacg cagagcaatg
     1321 tgggcacagg tattccgtta caccacgctt gataaagtgg tctttattaa acttcgttgt
     1381 ttgagcgccg ggaatctcag gcagtagcga agcgaccgga actttatctc cggtgagcgc
     1441 ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac tggaaagcat gttctaatga
     1501 cagcaatgct tcccgtagct tttcagcttc cagtccgtct tcccagatat catcccaacg
     1561 acgatgatct tttggcgcga agctggtttg tagtagcccc agcaaaaact gccataccgc
     1621 accctggaga tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg ccagatcgac
     1681 tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg gggttgtcag
     1741 aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg tcggttgtcc
     1801 atctttccat ccttttcggt gtatcaacta ttcagagagt cctgtcgcag acatctcaac
     1861 caaccttgcc aatcaatcct tccgtagccg aatacccaca actttcgtca tcgttgacta
     1921 aaataacgtt tgccatctcc ggatcttgtc gctgttcaat gcaccacagc ctgaacgcct
     1981 ctccctccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg cgcactctga
     2041 caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca ccgtcaatac
     2101 aggtagccag ccacagggaa acagactcct cagccagacg tgtcgacagt ttttctggga
     2161 ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacaaac tttttagcca
     2221 ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca aatccttccg
     2281 gcattaccac gtcctcaccg tagacggatt caataagaag ccgggctgcg tgtggcatct
     2341 gaatagcacc ttgctcacgc agcacacgct gcgtcagcca gattcgccca tgatcgggat
     2401 agacaaatgc actgttacgc atggcactgc caaaccattc gtcgccagga gagtcgtccc
     2461 agacgggagc cagaatcagc aattcagggg gggaacgctc gtcctttccg tcacgcttta
     2521 actgaccatt aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga atcagcaaat
     2581 caacaggggc caggtcggag atcatttcgt ccaggtcaca atcgacactc tgctctaata
     2641 cctgagtaca aataaggact tttccggcac gctgtgagct atcttcttta ccaaagcgtg
     2701 ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta tgaaaaagtg
     2761 aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatgaacc ttaatagcgt
     2821 catcgacaga attccggatc caggcgatgc atttcccctg acttaccgcc gattcgatac
     2881 gcgcaataca ctcttgttca ctatgaagcc aacccacgct gacgctacgc tcaacgtctt
     2941 tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt gacatgcgtc agccaggggt
     3001 aatcatcctt ttccaggaac ggagcttctt gcaggccctc tgtaccccgc gcaaaggcag
     3061 cgacgagttt gtcgcgctgc tgttgagata acgtggcaga aagcaaaata acgctgtttc
     3121 cgccacgcgc ctgccgctcg atcaaccctt caagaatgca cgacatgtaa gcgtcacagg
     3181 cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc agattattat
     3241 gtttgaacgg catcaccgcc atcatcgcct gatccagcgt gccgacgcca atttcagcca
     3301 gcaacgcctt cttgttactg tcggcaaacc aggccgcaca tccctgactg aacgtttgtt
     3361 catccggttc ttctgacccg actaaatcac cagaccagag tgattcatta aagcggtcca
     3421 ttaatgtgcg ggcactatgt gccagcacca ggctggggcg ggattctggc gaatagaaag
     3481 caagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt gttggcagac
     3541 caaaaaacag cccctgagcc ttccccgcag ctatcaacct gtgcgccagg ataagcgccg
     3601 cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt ccttgctggc
     3661 tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata aaaggaaaca
     3721 gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg gttaaggcgt
     3781 tttgagcctg aactaaagtt ttctgccagt aacctttaat atccattggg tgtgcgacgc
     3841 gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt gcagagatat
     3901 accaggaaag gtgttttata agttcaatgc cctcatcatc atcccagaat gcggggatct
     3961 ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca gctttgtctt
     4021 caggcagaaa attatccaat tcatccatac gatcaggggg ccaaccatga tgccctgtcg
     4081 tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc gaagatgaag
     4141 gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc agcgttgaat
     4201 gagagatttt ttcgtaattt tttctggcac cttcctgaat cttgagttca gggggcaggt
     4261 aaagttgttg aaaagagcgg gcaaattttc caatatcgtg ccagcacagc aaccaggcga
     4321 aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa tcagccagcc
     4381 cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc gaatgccagc
     4441 aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcgggttttt ccccagaaat
     4501 caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta cgcatatatt
     4561 aaaaattatc tgtgatatat atcattcaat aaaatattta cattatgaat aatcaaataa
     4621 ttttcatccg taatatatat gaataataaa cataggcata atattcacat aaataatata
     4681 aattaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg ttttaccccc
     4741 gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca taccacgttc
     4801 tctgatactc aactcacaca acgagtcgcc taccatcagc gtgaatccca gtactgtaaa
     4861 acacagcact atgattgcca caagggcata ttttgtcagc atgtcttgcc tccctgcaaa
     4921 gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg atagaaatat
     4981 cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac agcctcaagc
     5041 acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta aaatcaggaa
     5101 tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta atttatccgg
     5161 caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac gtgtttcttc
     5221 ttccgacatg ccaggttccc atttacgggt tgtatgggta tcaccgaccg ataaatatcc
     5281 ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat aaatagttcg
     5341 gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg ccagcaccgg
     5401 taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg tttgcgcgtt
     5461 aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt acttttcaat
     5521 gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg ctgcgctttc
     5581 ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt caataaagcg
     5641 gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat ccgggcgaat
     5701 ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag aaagtacata
     5761 ttcaccgggc agattatcca gcgcccaggc tacgcggcct tcagcgtcca gtttttccag
     5821 ttcggcgtta gtttccgcca gcgccagaat acgatctacc ttcggcagtt cgttcagggc
     5881 gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg cgacgctcac
     5941 gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc gcgcgccgga
     6001 tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc ttcacccgct
     6061 tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat ttccggctcg
     6121 gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc gccaagatgc
     6181 aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat cgcgcgacca
     6241 caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac accatgtttc
     6301 gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctctgc ttccgccatc
     6361 gccagcgggc aagtcgggaa tgacacgcag gccatcgagt tttcacgctg cggcgtgacg
     6421 gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc gctttccggt
     6481 acaccggtga tgatcagatt ctggttcgcc gtaatgcgga aatcgccttt gtggatcttc
     6541 gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat gcgaccattt
     6601 tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc aatacgatcg
     6661 cctcgtccgg tgaactcata tggacggatc ggttcaaatt tgatccccgc gcgacgctcc
     6721 acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt tttggcattt
     6781 ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc ggccaccgcc
     6841 agcgtatgct ccagcggcag atagccaaac tcgctcgccg tgcgggcgta ggttttcttg
     6901 ttgccgtgtt cgatagaaag cccaccgccc accagcaggt tgaagcctac cagcttgccg
     6961 ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc gttctgcggc
     7021 gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag gatcggttct
     7081 tcatcagtag tggcgacttt ttcctggtcg agccagatct ccgcatacgc gcgggtacga
     7141 ggcaacagat gctcagaaat cttcttcgcc cactcgtacg cttccgcgtg cagctgcgac
     7201 tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagctgt cgccagcgca
     7261 tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt cagaatgccg
     7321 tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt gttttcaccg
     7381 gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg cagacgacag
     7441 cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc acggatgtcg
     7501 cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttgtc gcccttaaag
     7561 ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta gttgctttca
     7621 agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc tggatgtttt
     7681 tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca cttaaaaatt
     7741 catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga agtgcctgct
     7801 caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca ccgtcattga
     7861 tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacataa actttttctt
     7921 tttggtcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg acgtagcgct
     7981 gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag aacagccagt
     8041 ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa ggcacggaac ggcgcgatgc
     8101 cggtaccggg gccaatcata atcactggcg tttgcgggtt agcgggcagg cggaaattgt
     8161 cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcgatcc gcgaggaagc
     8221 tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca ccaacggtga
     8281 cgtgtacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg cgcggggtca
     8341 gcgggcgcag caggttaatc agcgcttcgg catccagttg cgccggagag aaacgcacca
     8401 tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt cgctttatcg cccaccagcg
     8461 gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg gcggtgttga
     8521 cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg ccctcgacgg
     8581 tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc agtgccgggt
     8641 cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggcct gaatcgccta
     8701 agtcaatttc gatatgacga acgtcttttt cagagttacg cccggtaatt ttctggttaa
     8761 ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg atttcattta
     8821 ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat ttaagcgcat
     8881 caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacatcg gcatcgacac
     8941 ggtcgagcag gcgttcacca cccagttccg ccagcttgct gtcgaaatct ttcccggact
     9001 ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg gtgttttcca
     9061 gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct tccggcggtt
     9121 ccccttcccc ttgcgttgac gtcactacga tgagcagttt ttcgctggcg atttgtttga
     9181 atttatagtc gcccgcgttc accagcttaa cgttcagttt tgctgctaat aaatcatcgc
     9241 gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga ggcggagata atagttatac
     9301 ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc tgattgagta
     9361 cgccccagaa atagccagaa acccaggcaa gctgggtagg cgttaaatcg gtcgtggccg
     9421 cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt gggacctgtg
     9481 tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt cagcgcccat
     9541 aaaacagaag agaagataag gttaacgggg tgaacggtgt ggattaaaga cgggatagcg
     9601 ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa caaaatagat
     9661 taaccaaccc aatgaaaaac aaatgaattt agccaatcat taagataaat cagcgatttt
     9721 gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact ctacggcggt
     9781 tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt tcaccttcga
     9841 agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc tgcacgggca
     9901 ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg gctggattat
     9961 cgatttcgct gaactgaaag cggcgtttaa gccaacttat gagcgcctcg atcaccatta
    10021 tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa aatggatttg
    10081 ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag aaacctgcac
    10141 cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat ttcccttaag
    10201 taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga agacgactgc
    10261 gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg ctgcgcgcga
    10321 gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa aaatctttcc
    10381 ggcgggcggt tatataccca tgcactcgcg gaactcctcc cgcaatttca tctgaccgcg
    10441 cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgcccga tggcgtaacg
    10501 acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc acggttcgat
    10561 ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg agcgacggtg
    10621 gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga cgatattctc
    10681 cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga acgtcacggg
    10741 ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt gctgtcgctg
    10801 gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc agcgttgctg
    10861 ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac taatcaacaa
    10921 acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagtcg tgttccggca
    10981 agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat caaaaacacg
    11041 gaatcactgg agtatggtgc gcatctggag ccagaaggtg gcttgcacag tatgccggtg
    11101 caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg cgtcaatacc
    11161 ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cacaggcggc ggcacaaacg
    11221 ctaataagcg cctgccagca ccgcgagccg caaaatctgt ttgcgcttta tcatcacaac
    11281 gtagagcgca gcctgctatg ggatgttcta caacgttatc agcatgttcc ggcgcttttg
    11341 caacgccctg gctggtatcg ggcgtggcct gcgttaatgc aggatatttc ccgcgattta
    11401 tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggcg tcatttacgt
    11461 cgtcatggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg tctgtagccc
    11521 ataatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc gttgagcgtc
    11581 agatggtaca acggttgatt aacgcctgtc cggcaggtct tttttcgctc acaccggaag
    11641 gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt ttgctgtgcg
    11701 acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc acctaccgct
    11761 ttggataaat aataaggata atttatgccc ctcttacacc tactccgcca gaatccggtg
    11821 attgctgccg ttaaagataa tgccagcctg caactggcta tcgattctga atgccagttt
    11881 atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa gattaagaac
    11941 gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa taaagaagtc
    12001 gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac caaagcctca
    12061 atgttgaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt tattgttgat
    12121 tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga ttgtattgag
    12181 atcctgccag gcggtatgcc caaagtgctg ggctgggtga cagagaaaat ccgccaaccg
    12241 ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat taacgcgggt
    12301 gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa attactttga
    12361 cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc acatccgaca
    12421 ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc caggccagaa
    12481 ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt ttgtcaccag
    12541 tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg aaaataccgc
    12601 agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc ccgccattaa
    12661 cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca gatgtccgga
    12721 aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc gactgtagcc
    12781 cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg cctctccgcc
    12841 ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa catttttcag
    12901 atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca gttgttgcat
    12961 tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg aaaggcatag
    13021 cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg atttgcgcac
    13081 gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat gtaaacgcca
    13141 ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa caacgtccgc
    13201 aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca ctacaggctg
    13261 aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg ccagccagct
    13321 tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg ctgcatcctt
    13381 tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg cccatcaatc
    13441 agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat cgctggcatt
    13501 tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg ctggcgcatt
    13561 cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc aggcaagcga
    13621 acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa cagcgcgtcg
    13681 agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc cagcaaaaat
    13741 ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag atccagcgga
    13801 ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa gcgcagatct
    13861 gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata gcgcagccag
    13921 tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt accgtttttt
    13981 ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa acttcgcagt
    14041 agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt ttccgccagc
    14101 attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac ctcctacatt
    14161 tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg tcacaacaaa
    14221 atcaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc actgcgccag
    14281 cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg cggtggcaaa
    14341 accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa tgcccaccag
    14401 attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg ttaatgaact
    14461 gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa aacttcccag
    14521 caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg cgcccacaat
    14581 taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa tagtctgggc
    14641 gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga caaagaagac
    14701 gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga tgtgtttatg
    14761 ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc gatgcacgat
    14821 agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggtgatt ctggcgttcc
    14881 ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta gccagcgcca
    14941 ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca catagcctac
    15001 ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg gggaaaattc
    15061 agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa tcaaaatgcg
    15121 cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag caagcgtaat
    15181 cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga tccacccaag
    15241 aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc ccgccataaa
    15301 cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga cgccgagaac
    15361 ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt gaaaacggtt
    15421 aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt cattgtccgg
    15481 taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca tcgcggaacg
    15541 ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa tgccattagg
    15601 atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt ccagtttgct
    15661 ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc cgaggcgaat
    15721 ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt cctcttccgg
    15781 cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt tctgatagct
    15841 atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact cagtacgaat
    15901 acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg acacttcagt
    15961 ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca ctttatccgg
    16021 tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc gctggcattg
    16081 cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa tgcgagggtt
    16141 accttcagcc ataaagatca gcacgcattt tccgtctgca aaatgggtga agtgttgggt
    16201 gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca ccatgacttc
    16261 acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata tagccgtaga agaggttgtt
    16321 ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa gtgcaccttc
    16381 gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca cgtttttaat
    16441 gcgtgtgacg gtgccatcag ccaatactgc ttccagacca acgaccatat cttcgattgc
    16501 gccgtagagt gtggagaact gcccgatact gcgggttgct accaagccgc ccatctgcgc
    16561 cagcggcctt gactgcggag aatgccccgt ggtgtaacct ttttcacgca acgcattttc
    16621 cagcacctcc agcggaacgc cacattgcgc cgtcgcctgc atattctcaa tatcaatatt
    16681 aatgatttga ttcatggcgg agccgtcaag caccaccgag ttttccacaa cggtttccag
    16741 cccaccttca gtggcggaag cgccggtacg cggcacaccg ttaattttgt gcgcattcat
    16801 aaaattcagc acacgggata cttgctctgt ggaaccgagt tttacgaccg ctgccggaat
    16861 cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa tactgttctt
    16921 ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct ttagttggtc
    16981 gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa aatggaataa
    17041 tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat aaccaccatt
    17101 gaccactaat aaatgcccat tgacataatt tgaagccgga cttgcgagga atacagctgc
    17161 gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa gaacgcgctg
    17221 attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat agccaggagc
    17281 aataccattt acctgaatat tatattgacc tagttcatca caataagctt tggtgaatcc
    17341 ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta agtaagagaa
    17401 caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt ttgcagcttc
    17461 atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc agtcggcacg
    17521 accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca gaatatcaac
    17581 tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat
    17641 atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct ttgtttcgcc
    17701 gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc
    17761 aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga
    17821 gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg
    17881 taaggcaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc
    17941 tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga tcgtaatcac
    18001 agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca
    18061 acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag aaaagtaatc
    18121 cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata
    18181 tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg
    18241 ttagttttga cctggcttat ttacgttata tttttcaaat tcccatggcg aaatttatgg
    18301 gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc
    18361 tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg
    18421 cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa
    18481 tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga
    18541 ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg ggctggatgg
    18601 aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg atcttttctc
    18661 gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac agtgtggttt
    18721 acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg
    18781 ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca
    18841 gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac gccattctga
    18901 gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca gcgagctaca
    18961 tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga ggcattatca
    19021 ccacctacag taaagtgaaa tcccctaccc gcgtgattca aatcctttcc atcatcggtt
    19081 tgctggcgtt gacagccctg ctcgtcacga actctaaccc ccaatcggtc gcgatgggga
    19141 ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct
    19201 gccccggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg
    19261 tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg caagacaccc
    19321 tgcccgctgc agaagcctac cgcaatatgt ggctgatggg catggcggcg ctcggcatgg
    19381 tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa
    19441 tagctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat
    19501 catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa
    19561 tgtggtttgc gaaggtaaag gcttattaca gccgatgcac acgcctgatg ccgatactgc
    19621 agaacatcct gacgacgatt tatgggcatc attatgtttt gcaggtcacg atttgatgag
    19681 ccagtttgcc gggaataaag aagatattgt tggcatcggc ctgggctcca tccgttgctg
    19741 ccgtgcgtta ttgaaagccg acggcacgcc agctgcgccg ttgattagct ggcaggatgc
    19801 acgcgttaca cgcccttacg aacatacgaa tcctgacgtg gcgtatgtaa cctctttttc
    19861 gggttatctg acgcatcgct taaccggcga atttaaagat aatatcgcca actattttgg
    19921 tcagtggccg gtggattata agacctgggc atggagcgaa gatgccgcag tcatggagaa
    19981 gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg ttcttgggca
    20041 tatcacacct caagccgcgc tggcgacgca tttcccggca ggactgccgg ttgtttgtac
    20101 caccagtgat aaaccggtag aagctctggg agccggatta ctggatgatg aaactgcggt
    20161 aatttctctt ggcacttaca tcgcattgat gatgaacggc aaagccttgc cgaaagatcc
    20221 ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg
    20281 tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat
    20341 tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaga aagcctctag
    20401 tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga
    20461 accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg catggatata
    20521 tcgttcgata ttggaaagcg tggcgctgac gctgaagaac aattacgaca atatgtgtaa
    20581 tgaaatgaat cactttgcga aacatgtaat cattactggc ggcggttcga acaggatctg
    20641 tttatgcaga tttttgccga cgtgttcaac cttccggcac gccgtaacgc cattaacggt
    20701 tgtgcaagcc tgggggcagc gattaataca ggggtaggtc tggggctata cccggattac
    20761 gcaacggctg tcgataaaat ggttcgcgtg aaagatatct ttataccgat tgagagcaat
    20821 gccaaacgct acgacgcgat gaataaaggc atttt
//
LOCUS       DAJTXQ010000072        20711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000072 DAJTXQ010000000
VERSION     DAJTXQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.072"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO515_002492"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO515_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6576405.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO515_002493"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO515_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6576406.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO515_002494"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO515_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6576407.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO515_002495"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO515_002495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6576408.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO515_002496"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO515_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6576409.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO515_002497"
     CDS             complement(8214..9107)
                     /locus_tag="OO515_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6576410.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO515_002498"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO515_002498"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6576411.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO515_002499"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO515_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6576412.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO515_002500"
     CDS             complement(10238..11470)
                     /locus_tag="OO515_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6576413.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO515_002501"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO515_002501"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6576414.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO515_002502"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO515_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6576415.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OO515_002503"
     CDS             complement(14275..16539)
                     /locus_tag="OO515_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR6576416.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO515_002504"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO515_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6576417.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO515_002505"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO515_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6576418.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO515_002506"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO515_002506"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6576419.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO515_002507"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO515_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6576420.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5732 c         5045 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAJTXQ010000073        20483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000073 DAJTXQ010000000
VERSION     DAJTXQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.073"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            754..1182
                     /gene="uspC"
                     /locus_tag="OO515_002508"
     CDS             754..1182
                     /gene="uspC"
                     /locus_tag="OO515_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6576421.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1189..2613)
                     /gene="otsA"
                     /locus_tag="OO515_002509"
     CDS             complement(1189..2613)
                     /gene="otsA"
                     /locus_tag="OO515_002509"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6576422.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2588..3388)
                     /gene="otsB"
                     /locus_tag="OO515_002510"
     CDS             complement(2588..3388)
                     /gene="otsB"
                     /locus_tag="OO515_002510"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6576423.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3555..4541)
                     /gene="araH"
                     /locus_tag="OO515_002511"
     CDS             complement(3555..4541)
                     /gene="araH"
                     /locus_tag="OO515_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6576424.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4556..6070)
                     /gene="araG"
                     /locus_tag="OO515_002512"
     CDS             complement(4556..6070)
                     /gene="araG"
                     /locus_tag="OO515_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6576425.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6140..7129)
                     /gene="araF"
                     /locus_tag="OO515_002513"
     CDS             complement(6140..7129)
                     /gene="araF"
                     /locus_tag="OO515_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6576426.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7926..8429
                     /locus_tag="OO515_002514"
     CDS             7926..8429
                     /locus_tag="OO515_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6576427.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8508..8759)
                     /locus_tag="OO515_002515"
     CDS             complement(8508..8759)
                     /locus_tag="OO515_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6576428.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8874..8960)
                     /gene="azuC"
                     /locus_tag="OO515_002516"
     CDS             complement(8874..8960)
                     /gene="azuC"
                     /locus_tag="OO515_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6576429.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9223..9546
                     /locus_tag="OO515_002517"
     CDS             9223..9546
                     /locus_tag="OO515_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6576430.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9717..10214
                     /gene="ftnA"
                     /locus_tag="OO515_002518"
     CDS             9717..10214
                     /gene="ftnA"
                     /locus_tag="OO515_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6576431.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10252..10491)
                     /locus_tag="OO515_002519"
     CDS             complement(10252..10491)
                     /locus_tag="OO515_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6576432.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10682..11893
                     /gene="tyrP"
                     /locus_tag="OO515_002520"
     CDS             10682..11893
                     /gene="tyrP"
                     /locus_tag="OO515_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6576433.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11955..12620)
                     /gene="yecA"
                     /locus_tag="OO515_002521"
     CDS             complement(11955..12620)
                     /gene="yecA"
                     /locus_tag="OO515_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6576434.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12816..12902)
                     /locus_tag="OO515_002522"
     tRNA            complement(12816..12902)
                     /locus_tag="OO515_002522"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12866..12868),aa:Leu,seq:taa)
     gene            complement(12915..12988)
                     /locus_tag="OO515_002523"
     tRNA            complement(12915..12988)
                     /locus_tag="OO515_002523"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12954..12956),aa:Cys,seq:gca)
     gene            complement(13043..13118)
                     /locus_tag="OO515_002524"
     tRNA            complement(13043..13118)
                     /locus_tag="OO515_002524"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13083..13085),aa:Gly,seq:gcc)
     gene            complement(13270..13818)
                     /gene="pgsA"
                     /locus_tag="OO515_002525"
     CDS             complement(13270..13818)
                     /gene="pgsA"
                     /locus_tag="OO515_002525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6576435.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13875..15707)
                     /gene="uvrC"
                     /locus_tag="OO515_002526"
     CDS             complement(13875..15707)
                     /gene="uvrC"
                     /locus_tag="OO515_002526"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6576436.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15704..16360)
                     /gene="uvrY"
                     /locus_tag="OO515_002527"
     CDS             complement(15704..16360)
                     /gene="uvrY"
                     /locus_tag="OO515_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6576437.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16656..16832
                     /locus_tag="OO515_002528"
     CDS             16656..16832
                     /locus_tag="OO515_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576438.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16819..17043
                     /locus_tag="OO515_002529"
     CDS             16819..17043
                     /locus_tag="OO515_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6576439.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17110..17832)
                     /gene="sdiA"
                     /locus_tag="OO515_002530"
     CDS             complement(17110..17832)
                     /gene="sdiA"
                     /locus_tag="OO515_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6576440.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(18062..18814)
                     /gene="yecC"
                     /locus_tag="OO515_002531"
     CDS             complement(18062..18814)
                     /gene="yecC"
                     /locus_tag="OO515_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6576441.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18811..19479)
                     /gene="tcyL"
                     /locus_tag="OO515_002532"
     CDS             complement(18811..19479)
                     /gene="tcyL"
                     /locus_tag="OO515_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6576442.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19494..20480)
                     /gene="dcyD"
                     /locus_tag="OO515_002533"
     CDS             complement(19494..20480)
                     /gene="dcyD"
                     /locus_tag="OO515_002533"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6576443.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         5235 a         5217 c         4715 g         5316 t
ORIGIN      
        1 atttttatgc ggtttcaccg caccccgtga tgtcgccggg aagccccggt aaaaaataat
       61 tagcattaga atagttgcga taagctgcaa taagcagaac cacctttttg gtttaatatg
      121 tccttacaaa tagaaatggg tctttacact tatctaagat ttttcctaaa tcgacgcaac
      181 tgtactcgtc actacacgca catacaacgg aggggggctg cgattttcaa taatgtgtga
      241 tgcagatcac acaaaacact caattactta acataaatgt ataagtcata cttttgtttt
      301 gggtgtattt caatatgtta aaaagttttt cgctacgcta gcaagcaaaa atgaaacagg
      361 aataatcgaa atgggatgtt gcgcacagtt aaaataaatc accgtaaata atcatctgct
      421 ataaataatc actttcatgc aataccagat aagctatttt taaacagaca cttaccacac
      481 aacaaactaa ttaaatacaa agaaaggcaa caatcaactt aatgttaatg aaatgtttta
      541 catacattaa ccacttgttt aatgcggaga atcagtacac tattttctta taattacatt
      601 tgaaattata tgatcaccgg gagtgattga ttaattgtct ttactaataa tcagactaat
      661 atttacctgt ttgaccgagt tgggattgcg tcgtttctcc attaggagta aagctttaat
      721 gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat tcttgtcgct
      781 gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat cgccaggcca
      841 gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta caatcaatta
      901 gccgcgccga tgcttgaaga tttacgcagc gtgatgcaag aagaaacgca aagctttctt
      961 gataagttca ttcaggatgc ggggtatccc gttgacaaga cgtttattgc ctacggtgag
     1021 ttaagcgaac atattctgga agtatgtcac aaacatcatt tcgatttggt gatttgcggt
     1081 aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt gattgcctca
     1141 agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct acgcaagctt
     1201 tggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa ctatctgctt
     1261 taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga taacgtccag
     1321 catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac gatccagcgc
     1381 agctgcaact tcgtcacgat cgtaggggtt aacaattaac gccgacgtta actcgtttgc
     1441 cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct gagcagcaac
     1501 atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc ccacgtcaga
     1561 gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat aataaagcgg
     1621 cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt cgagctgatg
     1681 acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct gggtataacg
     1741 aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga gaaaacgctc
     1801 tggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta cgtttttcag
     1861 ttccgcttta agttgcgcca gttttggcgg cagtggcccg gcagcctgtt tggctatttc
     1921 tttcggttca atgccgatcg ggtagacttc tgttcgaaat gctttgcccc aggctgtatg
     1981 gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa agacaatcca ggaacgccag
     2041 acgatcgttt tctgtctgga aacccagcaa atcataatca caaagctgtt caagcaaggt
     2101 gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat gcagaaagaa
     2161 accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaatggca acaggtgata
     2221 atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat ctgccagcaa
     2281 cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgagccg
     2341 ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc
     2401 ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttcacct tttttagcgg
     2461 ctgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg cggctttcag
     2521 tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt ctggtggtgc
     2581 aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta ttttctcttt
     2641 tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc acacccgcca
     2701 gtcgccatga tgcctgagtt gcacctgtgc caatttttac tgacattccg cccagtcggt
     2761 taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc
     2821 cgataaaggg agcttcctgc ataaaagctg caattgcctc acctttactg gtacctctcg
     2881 gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag atctgagtaa
     2941 tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga taatgcagcg
     3001 caaaagccat ccctttcgcc tccagctccg cgccgggata ctgagcgatg actgtatgca
     3061 gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgt gttttaccat
     3121 tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca
     3181 gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc caatgcacca tcacttgcgg
     3241 ttgccagtag ctgtagtcct tgcagaatat tgtcaggcac gacgacctga tcgggatgcg
     3301 gtttgatttc cgccagcgtt ccatcaagat caaaaaacca ggcatatttc gcggatagtt
     3361 caggggtttc ggttaacggt tctgtcaccc ggctctcctc cttctctttt cttattgttt
     3421 tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg tcgccatttg
     3481 caatgaagaa cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac gttaaattgt
     3541 tgtggaaaaa agcatcagac aatgcgtttc gctttttgct tgtaacggtc gaagatcacc
     3601 gctgccagca ggattaagcc gcgaaccacg tactgcgcga aaggagaaat attaagcagg
     3661 ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata tgagattttt
     3721 ccgatgccac ctttcagaga aacgccacct aaaacgcagg cggagataac aataagctca
     3781 taaccaatcg acgtcattgg ctgcccacta gtcatacgtg aagccagaat aattccggct
     3841 atcgcagata ccaggccaga gagaacaaag ataataattt tggtgcgaac aaccggtaca
     3901 cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca gggtgttacg accaaaggtg
     3961 gttttattca gcaacaaacc aaagataatc agacacgcga cggtgagcca gattggcgca
     4021 ggcagaccga accagttagc gtaaccaagg gcaaagaagc tttcatcttc gataccgacc
     4081 gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtt
     4141 gtgatcagag catttatttt cagtttggcg ataacaaagc cattgaccag gccacagaga
     4201 atgccaagca gcaaccccgc tgccacgcca atccacaggc tttcagtcag gttgataacc
     4261 accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc aaagtcaccg
     4321 gaagcaaggc agaacaacat gccacacgcc accatccccg acatggaaat tgccaggccc
     4381 aaccctttca tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca ggcgataaag
     4441 agcaccgcaa acaccaccag catgccgtac tgatcccaga tacgcccgaa gctgaatgac
     4501 gacttaggtg cgccagaacc cgatgtagaa acagaagaca tcatactctc cttactcagg
     4561 caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct
     4621 cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac cacaatccgg tcggcaacgc
     4681 cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc gccgccagcg
     4741 cataaattac gttatatatt tcgtgcttag caccaacatc aatgctgcgc gtaggttcat
     4801 ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct ttttgctgat
     4861 ttccgcctga gagattcatg atcagttgct cagcgcccgg cgttttgatg ttgagcgaac
     4921 gaatgtgctg atcggcattg ttttcttccc aaccgttgtt gattacacaa ccgccgagca
     4981 catgtttgcg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg ggaataatgc
     5041 cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg tggctcggtt
     5101 tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc
     5161 caaacaagcc tttcattaat tcgctacgcc ccgcccctac cagaccaaac agcccaacaa
     5221 tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct ttcacagcat
     5281 caagacgcag gcgctcctcg ccataactac gaggttgcca gccgtagata tcgccaatgt
     5341 cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg
     5401 ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca aatatttctt
     5461 ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca cgaataacgc
     5521 ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca aaggcgataa
     5581 ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga ccaatggaga
     5641 gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt tgtaaacccg
     5701 cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc tggccgagat
     5761 agatgttttc cgcgacggtc atttccggca cgagatgcag ttcctggtaa ataatcgcca
     5821 cacccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta
     5881 cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt gattttcctg
     5941 cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa ctaatatccg
     6001 tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga tacggggtag
     6061 actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc atcatgcaga
     6121 ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt tcttctttaa
     6181 agttgtcacg cgtgatcagc accacgtcgg taacttcggt aaattttggc ggttcaacgt
     6241 cttttgctac ccagttgtaa agcatttcgc tggatttata gccatgtacg tccgggcttg
     6301 gcagcaggga accgtagaag ccggttgcct gtgctttaga cagttcgctc aacgcatcca
     6361 caccgttaat gccaatgccg atgatatcgg cagctttaaa gccctgacct tccgtcgcgc
     6421 gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt ttaacttccg
     6481 gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg
     6541 taggtacctg ataaatttgt ttttccggga atccggccgc tttcagcgca tccatagaac
     6601 ccgttgtacg gcgacgtgcc gtatccagtt cgttggcggt aatcgccatc accgcgcttt
     6661 ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctgaccc tgacgttcgc
     6721 caattttagt cgccgccatc atcaccagcg gaacggtatc cattggctta cctttggcgt
     6781 taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct ttcgcgacga
     6841 tggcagagcc gagtttgggg tccggagtac aaataacgaa accttttgcg ccactggcag
     6901 ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc ttaataacct
     6961 caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg aaccacggct
     7021 cttccggttg cttcaccaga aaaccgagct tcaggttctc cgccatagcg gattgtgaca
     7081 taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg gttctctcca
     7141 gctttagtgt cgttttgtgt agggcaaaaa cgaatgactt tcgttaaatt aatcggaaaa
     7201 caaagcatta ccttttatct aaaagataag tgaccgtgtt gacatagttt tagcgagaaa
     7261 ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac gtgcataaat
     7321 ttagcgggaa aagacataag ggaaagccaa tttgtcagac aaattgtcga atgcacagca
     7381 gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat gcgggatgtc
     7441 ttctttttaa ccagtcaata ggccgcatta cctggcgttg agtttttgaa atggtgtaat
     7501 aaccgcaact caaagatgtg gaaaatgcac gtcattcatt tcgccattaa ttatcactgt
     7561 gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga aaaaacgttt
     7621 tataaaatca tcacttcatc atgaattcaa attcattgat taatatcaac aagatacaaa
     7681 aagcactatc attaaaattc attgcagtta cattgatttc atcaatgaaa tgtaaaaata
     7741 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga
     7801 tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca
     7861 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
     7921 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
     7981 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
     8041 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
     8101 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
     8161 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
     8221 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
     8281 tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg
     8341 atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc
     8401 tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg
     8461 aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca
     8521 ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg
     8581 accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt
     8641 ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc
     8701 aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc
     8761 gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata
     8821 gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg
     8881 aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc
     8941 aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct
     9001 ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata
     9061 aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc
     9121 tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt
     9181 aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg
     9241 actctttcat taataacgct ggcggggtgt acggttactc gccaagccca tgtgagcgaa
     9301 gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc
     9361 cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat
     9421 acccacgcgg tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta
     9481 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
     9541 ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt
     9601 tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg caaataataa
     9661 aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc
     9721 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
     9781 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
     9841 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
     9901 ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
     9961 cacttgatga attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta
    10021 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcagt
    10081 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
    10141 gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg
    10201 acacacaaaa ctaatgctct ccggcggcag gtttactgcc gccgtttcaa atcagtgacg
    10261 gcaaatctta ctctgatcgg tcgaaaaacc gtctttcgaa ggtataaatt tgccttttgc
    10321 tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcagg tgtgaaaacg
    10381 tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg tatattgctc
    10441 gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca tcgttcctcc
    10501 tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag cgcaggactg
    10561 aatgcttttt attgtacatt tatatttaca ctatatgtaa cgtcggtttg acgaagcagc
    10621 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
    10681 cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
    10741 catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt taatcttgtt
    10801 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtaccagca
    10861 tgttccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca
    10921 atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag
    10981 cggtgtcggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
    11041 aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg ttggaacatc
    11101 gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat
    11161 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
    11221 ggggctggct ctgtcagcaa tcccggtgat ttttacctca tttggttttc acggtagcgt
    11281 gccgagtatt gtcagctata tggatggcaa cgttcgtaag ctacgctggg tgtttataac
    11341 cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcaacgc ttggcagcat
    11401 tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg ggctgttaca
    11461 ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga
    11521 tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt atctggctga
    11581 tttatttcag cgttcaaata ccgttggtgg acggttgcaa actggtgcaa ttacctttct
    11641 gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
    11701 cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag
    11761 aaagcacaat cctcaggcgg gttaccgggt caaaggtggt cgtccggcgc tggtggtggt
    11821 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag ggttgttacc
    11881 agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt aacgctgaca
    11941 cccaatttgg cctcttaatg caggcagcac tgcttaaatt tcttaccact accgcacggg
    12001 caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac agctttttcc
    12061 tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg
    12121 ctctcttcaa acgcttctgg cgacatcttt tctacccgct cgaagttttc ctcagtaccg
    12181 tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg gcaacgttga ccagtcagaa
    12241 agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag ctcgctgcca
    12301 tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt cagacgctct
    12361 gcggtatcgg ccatatgttg aaaagccagg ttcataaagc gcgtcatctc tttctctgac
    12421 gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg ttccggttca
    12481 atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc cacatcaagg
    12541 atggcgtggt tagtgttgta tttggtcaga atatcgtcca gccattccaa ctcactttcg
    12601 tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca gcacctatct
    12661 tacatgccgg tccgtatcag agatactttt tgagtggctt tgccggtgat taaaaattaa
    12721 ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac
    12781 tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg gacttgaacc
    12841 cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc caccatccgg
    12901 gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg atttgcaatc
    12961 cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc agacatccgc
    13021 ttgacgccga tgtcttttaa actggagcgg gaaacgagac tcgaactcgc gaccccgacc
    13081 ttggcaaggt cgtgctctac caactgagct attcccgcat tcatcaagca atcagttaat
    13141 cacttgattt tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg cattctactt
    13201 acctggcgcg atgagtcaac gatatttttc accacttttg atcgtttgct gaaaattacg
    13261 ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga
    13321 ccacagagtc agtaccgcag ccacaaagaa aagtgcaata ccggcgtact caacccaaat
    13381 gttcggacgc cacagcagcc atgccaacgc caccatctgg gcagtggttt tcactttccc
    13441 aatccaggag acagccacgc tactgcgttt acccaactcc gccatccatt cgcgtagcgc
    13501 agaaataata atttcacggg cgatcatcgt tgctgccggt aaggtcaccc accagctgtg
    13561 gtaatgctcg gttaccagca ccatggcgat agccacgaga actttatctg ctacagggtc
    13621 aaggaaagca ccaaaccggg tactctggtt ccagcggcgt gccagaaaac catcgaacca
    13681 gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca aacggcgacc aggtgacagg
    13741 cagataaaag accaatacaa agaatgggat aaggatgaca cggaacagtg taagcaacgt
    13801 agggatatta aattgcataa tgacgggtaa ctatctgttg tcagtaagat tacccctatg
    13861 ttgctacaga gacatcaatg tttcaacgac cagaagatct tttctgccag accttgcgaa
    13921 atacccggca cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc
    13981 atatatttca acaacatttg ccgacgtttt ggcccgacgc cttcaatggt ttccagggaa
    14041 ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc caatcgcgtg atcatgtgat
    14101 tcatcgcgaa tatgctggat aacatgcagc gcgggagaat ctggcggcaa actgaatccc
    14161 tcaccttccg gctcaaagaa cagcgtttcc agcccagcct tacgatctgc tcctttggca
    14221 acgccaagta gcagcggatg atttttatcc catgagacat ccagttcggc gaagacattt
    14281 ttcgcctgcg caagctggcc tttgccgccg tcgataagga tcacatccgg gatcttactg
    14341 tcgtcaatgg ctttaccata acgccgacgc agcacctgat tcatcgccgc ataatcatcg
    14401 cccggcgtga tgcctgtaat gttatagcgc cgatactccg cacgcagcgg gccgttagca
    14461 tcaaacacca cacaggaagc gacggtttgt tcgcccatgg tatggctgat gtcaaagcac
    14521 tccatccgct tcacttccgg caatttcaac acgctggcaa gcgcggtcag tcgctggtga
    14581 acggtagatt gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt gcgcgcgagt
    14641 ttcagataac gcgccctatc gccgcgaggt ttggtttgaa cattaatctt gcgtcccgcc
    14701 agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc
    14761 tcacccggta aggtgcgcat ctggctgcct tgtaaataga actggcctac gaaggtttct
    14821 accacctcgc tcagttccgt accgccaggc actttcggga aatagctgcg gctgccgagc
    14881 actttgccct gacgaatgaa caatacgtgg acacaagcca tgcccgcatc gaacgccaca
    14941 ccaataacgt cgaggtcgtc gccggtattg gaaacgaatt gtttttcggt gacgcgtcgc
    15001 accgcctgaa tttggtcgcg aatacgtgca gcttcttcaa actccagatt ctggctggca
    15061 gtttccatac gactaatgag ttgcgtaagc acctgatcat ctttgccaga caaaaacagg
    15121 cgcacatact caacctgctg agcgtattct tcttcactca ccagtccttc aacgcacggt
    15181 cccagacagc gccctatctg atattgcaga cacggacgcg agcgattgcg ataaacacta
    15241 ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg ccagtgtttc acgtacggca
    15301 tagccattcg ggaacgggcc gaaatattca cctttggcat gcttcgcacc acgatgcatc
    15361 gccagacgcg ggtgggtatc gccactcagg aagataaaag gataagattt atcatcgcgt
    15421 agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt tgtgttccag caacagcgct
    15481 tcggtttctg tatgcgtaac cgttacatca atttgctgga tctgggcgac cagcgcttcg
    15541 gttttgcgcg aagcgaggtt gctgcggaaa tagctggaaa gccgtttttt caggtctttc
    15601 gctttgccga catagataac cgtaccacca gcatcgtaca tgcgataaac gcctggctgg
    15661 caggttacgg tttttaaaaa cgcttttgcg tcaaactgat cactcactga cttgataatg
    15721 tctccgcatt acacagacca tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa
    15781 tgtttagttt actgaacata cgatagcggt agctgttcac cgttttcgga ctgagattga
    15841 gctgttctga gatctcattg accttctggc ccttggtgat catcagcata atctgcaatt
    15901 cacgttcaga caaactggca aatgggcttt ctgttttttc tggttcgatc tggcttaacg
    15961 ccatttgttg agcgatatca gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg
    16021 cactcacgac ttcctgcgga gccgcgcctt tgctgaggta gcccgcagca ccggcctgca
    16081 tgactttcgc tggtaaaggg ttttctgtat ggacggtaag catgatgatt ttgacatcag
    16141 ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc aatgcccggc atactcatgt
    16201 ccattagcac cacgtcaacg gcatttgtcc ggcaccactt aacggcgtct tcaccgcacg
    16261 atgcctcacc gacgactttt atacccttta tatcttccag aatgcgtcgt atccctgcgc
    16321 gcaccagttc gtggtcatca acaagtagaa cgttgatcaa aggaatatct ccagaaatag
    16381 ggataacgct actgatagtt agtcattcgt atattagcgg tttttattgc aactttgaaa
    16441 cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg taaattcgat tgtccacagt
    16501 tatggtcacg cccgtcacca acggcttccg ggatcgtttc acagtaagcc cggcgatgaa
    16561 atgtgacaaa aattgacttt attcagcaaa aatgaaaatc agcccgctga atatgtaaca
    16621 ttaattaacc cgaacaatac gggcaaaaaa cattcatgat aaaaatattt attggtcatt
    16681 atattaacgc atttaatagc attgttgacg tcacgctcaa aaaacaacca ctgctatttt
    16741 tagcaaagct tatggtatac tccgccgcct taacattttt caccgcaaat tttcattgca
    16801 acatgacgag gaaaataaat gagtacgcct gatttttcta ctgccgagaa taatcaagaa
    16861 ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc tgatgctaca ggcgatggga
    16921 caagctgacg cgggccgcgt gatgttaaag atggaaaaac agcttgcgct gatcgaagac
    16981 gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag
    17041 taatataaaa ccggctgaga gcgtgccttt cagccgattt ttgcatctgg cacgcagaaa
    17101 gaaaagcgat caaattaagc cagtagcggc cgcgtaacag gccacctggg tcttatttgg
    17161 tgcattgatt tttttctgca tgtttttctg atggaaattg accgtattct cagagattga
    17221 caaaatcatc gctatctctg ctgatgtctt cccttccgcc gtccatttca gaatttcttt
    17281 ttcgcgcttg ctgaaattca tctctggcgt catcactatt tcatcattta aacgcatcag
    17341 agccatcaga ctttcgcgca ccagtaactg catttttaat tgcagttcat cactaagaat
    17401 gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc gatttggcag
    17461 cattaaatac tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt cccataatgg
    17521 ctgtgcttcg ctgaataagt catcattcca cattaaatgg ccctgactaa agttttcagg
    17581 gttcagcaca ggatcaattg cgagaaaatt ttttgcctga taataactaa cccacgactc
    17641 agggtaattg gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt ggcgaacaca
    17701 taacgaatag taatcgtact ccagctgctg agcctgaagc tctatttcat ggtagacctc
    17761 ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc tgaaaaaatc
    17821 cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag tatcatttat aatgataatt
    17881 caaactatca cataaataag atttatatat aatttatatt attcaggcaa tgaattactt
    17941 ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt
    18001 tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt
    18061 attattgcag tagaaacttc tcgaggaact ggcgggtgcg aggctgctgg ggatcagcaa
    18121 ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaaa atcgcccggt
    18181 ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct
    18241 gcgccagctg gcggattgtg ttcaggactt cgcccaccag ctctggatcc agcgctgacg
    18301 ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca atcgctacac
    18361 gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa
    18421 ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga
    18481 tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct
    18541 ggaagacaaa cccgacgtgc tgacgcaact ggcggatcag cgatttttgc tgacttaatg
    18601 aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca
    18661 gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg ataattgcca
    18721 ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtgaaatt
    18781 ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag
    18841 ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc agtagatgag
    18901 cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg
    18961 acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatccttta ccaggctgat
    19021 aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg
    19081 catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac ctttatcaat
    19141 agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg gcagtgttca gcgacagacc
    19201 aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat
    19261 cataaacagt tgtgcgatga gcggtgtacc acgaaaaata gagatataaa aacgcgccag
    19321 ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagcagtaa
    19381 gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt tcagcaggaa
    19441 cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg
    19501 tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag aatcggccct
    19561 tcatctttga agcgtttctg actgatacag ccaatcagcc ccgccatcgc ttttccggta
    19621 tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct
    19681 tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtat aatttccgct
    19741 gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaac
    19801 tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt
    19861 tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac
    19921 gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct
    19981 agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct
    20041 tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc ttcgcacatt
    20101 tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa atagttttct
    20161 gcggttgtgc caataggatt ttccagcagc gccacacagt gcagaccgag tttcgccgca
    20221 actgcggcag tctgccgcac atggttagac tggatcaccc cggcagtaat cagcgtatcg
    20281 gcaccttcgc gcagagcatc tgccgcgaga aattctagct tacgtaattt attgccgccc
    20341 attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag ataatcagaa
    20401 aagcgcggca gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg
    20461 gttaaattat gcagtggcat aac
//
LOCUS       DAJTXQ010000074        20444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000074 DAJTXQ010000000
VERSION     DAJTXQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.074"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..909
                     /gene="ypdA"
                     /locus_tag="OO515_002534"
     CDS             <1..909
                     /gene="ypdA"
                     /locus_tag="OO515_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6576444.1"
                     /translation="QNGDDFISPTTRQAMNYGKIIIKNNDEAHRTPEIHSMLVIPLWE
                     KGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRAEQLREMANKAELRALQ
                     SKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIELKDDEQIDIKKELYQI
                     KDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAIVHGIQPCKGKGVVTIS
                     VAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNVHHRVKLLYGEGLHIRR
                     LEPGTEIAFYIPNQRTPVASQATLLL"
     gene            924..1658
                     /gene="ypdB"
                     /locus_tag="OO515_002535"
     CDS             924..1658
                     /gene="ypdB"
                     /locus_tag="OO515_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6576445.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1671..2528
                     /locus_tag="OO515_002536"
     CDS             1671..2528
                     /locus_tag="OO515_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6576446.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2531..5026)
                     /gene="ptsP"
                     /locus_tag="OO515_002537"
                     /pseudo
     CDS             complement(2531..5026)
                     /gene="ptsP"
                     /locus_tag="OO515_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5051..6088)
                     /gene="ypdE"
                     /locus_tag="OO515_002538"
     CDS             complement(5051..6088)
                     /gene="ypdE"
                     /locus_tag="OO515_002538"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6576447.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6088..7173)
                     /gene="ypdF"
                     /locus_tag="OO515_002539"
                     /pseudo
     CDS             complement(6088..7173)
                     /gene="ypdF"
                     /locus_tag="OO515_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7188..8435)
                     /locus_tag="OO515_002540"
     CDS             complement(7188..8435)
                     /locus_tag="OO515_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6576448.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8457..8783)
                     /locus_tag="OO515_002541"
     CDS             complement(8457..8783)
                     /locus_tag="OO515_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6576449.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9003..9968)
                     /gene="glk"
                     /locus_tag="OO515_002542"
     CDS             complement(9003..9968)
                     /gene="glk"
                     /locus_tag="OO515_002542"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6576450.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10172..11428
                     /locus_tag="OO515_002543"
     CDS             10172..11428
                     /locus_tag="OO515_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6576451.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11543..11869
                     /locus_tag="OO515_002544"
     CDS             11543..11869
                     /locus_tag="OO515_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6576452.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12010..13248)
                     /locus_tag="OO515_002545"
     CDS             complement(12010..13248)
                     /locus_tag="OO515_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6576453.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13584..14786
                     /gene="nupC"
                     /locus_tag="OO515_002546"
     CDS             13584..14786
                     /gene="nupC"
                     /locus_tag="OO515_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6576454.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14836..17024)
                     /locus_tag="OO515_002547"
                     /pseudo
     CDS             complement(14836..17024)
                     /locus_tag="OO515_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17233..17308)
                     /locus_tag="OO515_002548"
     tRNA            complement(17233..17308)
                     /locus_tag="OO515_002548"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17273..17275),aa:Ala,seq:ggc)
     gene            complement(17348..17423)
                     /locus_tag="OO515_002549"
     tRNA            complement(17348..17423)
                     /locus_tag="OO515_002549"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17388..17390),aa:Ala,seq:ggc)
     gene            17644..18003
                     /locus_tag="OO515_002550"
     CDS             17644..18003
                     /locus_tag="OO515_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6576455.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18005..18397
                     /locus_tag="OO515_002551"
     CDS             18005..18397
                     /locus_tag="OO515_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6576456.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18449..19864)
                     /gene="gltX"
                     /locus_tag="OO515_002552"
     CDS             complement(18449..19864)
                     /gene="gltX"
                     /locus_tag="OO515_002552"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6576457.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20123..20198
                     /locus_tag="OO515_002553"
     tRNA            20123..20198
                     /locus_tag="OO515_002553"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20156..20158,aa:Val,seq:tac)
     gene            20244..20319
                     /locus_tag="OO515_002554"
     tRNA            20244..20319
                     /locus_tag="OO515_002554"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20277..20279,aa:Val,seq:tac)
     gene            20366..20441
                     /locus_tag="OO515_002555"
     tRNA            20366..20441
                     /locus_tag="OO515_002555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20399..20401,aa:Val,seq:tac)
BASE COUNT         5077 a         5458 c         5077 g         4832 t
ORIGIN      
        1 cagaatggcg atgacttcat tagcccgact acccgtcagg cgatgaatta cggaaaaatc
       61 atcattaaaa acaatgatga agcccaccgc acaccagaga ttcattccat gctggtgatc
      121 ccattgtggg agaaaggggt cgtgaccgga acgctgaaaa tttactactg ccacgcgcat
      181 cagatcacct cgtcattaca ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa
      241 ctggaggttt cacgcgccga gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc
      301 ctgcaaagca aaattaatcc ccattttctg tttaacgctc tgaacgctat ttcatcgtca
      361 atccgtctga atccggatac cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc
      421 tataacattg aattaaaaga cgatgagcaa atcgatatca aaaaagagct gtatcaaatt
      481 aaagactata ttgccattga gcaggcccgc tttggtgaca agctgacggt tatctatgat
      541 attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt ggtggagaac
      601 gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca
      661 gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat cgatccaaag
      721 gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct gctgaatgtc
      781 catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct ggagccgggg
      841 acggaaattg cgttttacat tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta
      901 ttgctttgag ccggagtgat attgtgaaag tcatcattgt tgaagacgaa ttcctggcac
      961 aacaggaact gagctggcta attaaagagc acagccagat ggagattgtc ggcacctttg
     1021 acgacggtct ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc atttttctgg
     1081 atatcaatat tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc cagttcgccc
     1141 ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac
     1201 tggaggcgtt tgactacatt ctcaaaccgt atcaggagtc acgtattacc gggatgctgc
     1261 aaaaactgga agcggcctgg caacaacagc agaccagcag tacgcctgcc gcgacggtaa
     1321 cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aataatcgtc acgccaatta
     1381 acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat
     1441 cctacgtaat gccgatgaac attaccgaat tttgcagcaa actgccgccg tcgcattttt
     1501 tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt
     1561 ttaataacac ctacattctg cgactgaaag atctggattt tgaagtgccg gtcagccgca
     1621 gcaaagtgaa agaatttcgc cagttaatgc atctttaatc gggaactttc atgaaagcac
     1681 caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggcctg gtgcttaacc
     1741 cgcaactact ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt
     1801 tatgtattga ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg
     1861 aagcaaagca gcagcgaatg gtggaaggag aaatgctgtt tattccggca cgcgcggcta
     1921 atttaccgat caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc
     1981 ttgggttatc gttttacgat agccgcacca cgtcgttgtt gcatcctgct cgccagatcc
     2041 agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc acccatctta
     2101 gccgttcgcc gctggagcag aatatcattc agccactggt gttaagtttg ctgcatcttt
     2161 gccgcaacgt ggtgaatatg ccgccgggta attcgcaacc gcgcggcgat tttctctatc
     2221 acagcatttg taactgggtt caggataatt atgcccagcc gctcacccgc gagagcgtgg
     2281 cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag catggaacga
     2341 tgcgttttat cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga
     2401 aatatcatct gtcaattcat gaagtggcac agcgttgcgg ttttccggat agcgactatt
     2461 tttgtcgcgt tttccggcgt cagtttggtc tgacgccggg agagtacagc gcccgttttc
     2521 agggctaacg tcagaaggtt aattctgttt ccagcagcgt caggatactt tgtgcatctt
     2581 gcgcggcaaa caacgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga
     2641 agactttcac atgattcatc ccttcgttag cacccagcgt cagcatgatc accagttcaa
     2701 cttcgcccat ttctgactgc cagtcaaccg gtttcgccag ccgggcaatg ctgatactgg
     2761 aatgacggat ccactgagat ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg
     2821 taacaatctc ttcccgctgc cagacatctt cttccagctc aaacggatgt tcagtgcgcc
     2881 cgttaacgcc gaggttgccg cacaggaact ggatcgcctg ctctttattg ctaaaatcct
     2941 gatcaacaaa gatattctcc agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg
     3001 cggtgagtaa cgcttcaatt tcctgcgcac tgcggcattc acatgcctga cgcgccagtt
     3061 cccgacacgc ctcgctatcc agttgacgaa gctggctttt caccgccgga atacgcgggc
     3121 tactcatact cagctcatcc aggcccagcc caagcagtag cggcagataa cggctttcac
     3181 cgcccagttc accgcaaatg cctacccatt tgccccgctg atgcgcagtg gtaactattt
     3241 gctgcaacat gcgcaggaac gatggcgtaa tcgggttata tagcggcgat acgcgcgggt
     3301 tattacgatc gaccgcatac agatactggg tcatatcgtt ggagccgata ctgaagaaat
     3361 cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc accatgatcc
     3421 caagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt
     3481 ggatctcgcc tttcacccat aagatctgat cgaggctgtt aaccatcggg atcatcaact
     3541 gggcgttgcc gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag
     3601 caaattccgg gtaaatacgt accgcgcgat agccgaggaa cgggttctct tcctggggaa
     3661 tattcagata aggaatgctt ttatcgccgc cgatgtccat cgtgcggaag ataatcggct
     3721 tgtcgcccgc cgccagtagc acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg
     3781 cgctgtcgcg atccatatag agcatttcgg tacggaacag cccgacacct tccgcgccgt
     3841 tggcaaacgc gcctggcgct tccagagcgg tgccgatatt cgccgcaata tcaatacgct
     3901 tgttatcacg ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat
     3961 ccgccagcgt ctgcgcgacc tgataataac cgctcaccgc gtcattcggg ttaatcgcca
     4021 gcacgccgca ctgggcgtca agcactgcag gttgcccggc ataacgggca atcgcatcca
     4081 gcggcaagcc actcagtacc gggatcgccg aggcacgggc cagaatcagt gtatgcgagg
     4141 tgcgcccggt tttctccaga atcatgcccg caagattttt caaatcaagg ctcaaaaact
     4201 ggcttggggt taaatcttca gccaccagaa tggtcggttt ttccagcacc agattgttgc
     4261 gcggcttcag ttccggctag gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc
     4321 tgacccgctc gcgcagataa tcgctggcag aagcagatag tttggcgcaa acctgctcca
     4381 tattgctgat gatcgccgcc cccagtccct gatgctgttc tgtcatcagg cgacggatat
     4441 tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgagc gctgaggata gttttgcttt
     4501 cgccgtcacg ctcacgcaat tgctggttca gttgctcggc aagcgttgcc aggctgtgct
     4561 ccagccgggt ggaatcttgc gcactggcgg ggattgcccg ataactgtcg aggctgtcgc
     4621 tttgtaacag ggtcagcgta cccacgccga cgccgcttgc cagcacattg ccgtacagta
     4681 aatccgggtt aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg
     4741 tcggctgaac gctgtcgctg tcgataaagc gcaccaggat gtactcttcc agcacgcgcc
     4801 gcgcctgctc ttcatcgctg ccgctaatgt tcaggctgca actgtcatta aataaggtgc
     4861 cagttccaat caacgccagc gagcttttag catcggcctt tgcgttctgg cgatggttga
     4921 taaaggtgat ttcgctttgc cactggctgc actgttcttt aagttcccac gccggacgag
     4981 catgtagacc gttaggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt
     5041 aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta agacgttgaa
     5101 taagggcaga taaaagttgc tccatctgca aaatgtcgcg gcaatcggca atcgatgccg
     5161 cgcaatgtcc gtggcgggtg gcaggcccca ttaccactgt gggtacgcca gtatcggtta
     5221 agtgcaccgc tccaccgtcc gtgccgccgt tactgaacat atccgcctgc aacggaacgc
     5281 caatttctgc cgacacggtt tcaatccacg cggtaagttt tggcggcgca atcagcgatt
     5341 tatcgcttaa caccagcatc ggcccgttac caatctgacg atggttagcc gcgccataat
     5401 caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacgtccggc gacaccgcgc
     5461 gggtagcagt ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc accagccaca
     5521 cttccgcagg cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc agatagcaac
     5581 cgaggcggtc atcaaaggct ttccccatca cccgctggtg agggagaacc tgaaaagctg
     5641 tatcaaaggt gacgcgatcg cctggacgaa ttcccgcctg catcacttcg tcatacgagc
     5701 gcgcaccaat gtccacgcgc atggcgctga cgtcattccc ctgccggtcg ccgtcaagca
     5761 ggcctggaat tttgcactct tcacgggtgg tgatgcgcac cggctgcagc tggcgggcag
     5821 ccatgcgcac gttgccaacc ggcagcacat caattgcccc ttcgcgggag atgctgcgca
     5881 ccataaatcc cacttcgtcc atatgcgcac agatcatcac cttcggacct gtcgattcat
     5941 tgaggcggat cagcaccgat cccagaccat caaatcgtac ttctttttgc aggcgatccg
     6001 cttcttccag taggatctgc cgcacttcct gttccgagga ggcgatcgca tctgcctcgc
     6061 tcaacgcttt taatagcgat aaatccatta tgcctctccc gtgagcaaca ctgttttcgg
     6121 catggcgtag agcacttctg cgccttgcgg ggtaaccagc acaacatctt cgatgcgcac
     6181 gcccccttgc cctggcaaat aaatccccgg ctccacggtc agtaacatgc ctggctgtag
     6241 cgtcgtggta tcccgcggtg aaaagcgtgg atcttcgtgt acttcaatac cgatagcatg
     6301 accggtgttg tgaccaaaat agtcgccaaa tcctgcctcg gtaatcaccc ggcgcgcggc
     6361 gtcgtcaacc tgttagcagc gtacgccagg gcgaatcgcg gagattgccg ccagctgtgc
     6421 ttgcaggaca atctgataga cgttaaacag cgggtgagat tcggcaatta ccccttcgcc
     6481 attcaccagc aaggtgcgcg tcatatcaga gcagtacccc tgatagagcg caccaaaatc
     6541 aagagtgaca aactcgcctg ctgcaacaat cttgtcgctg gctttgccgt gcggcagcgc
     6601 cccacgccag ccgctggcga caatggtgtc aaaagaggct ttttctgcgc cctgctggcg
     6661 cataaaccac tccagttcag cggctatctc gcgctcgctc atccccgcct gaataaagcg
     6721 gcgaatatgc tccgctccgc gatcggcaat cccacaggcg aggcggatta tctccacctc
     6781 ctctggcgtt ttgatttgcc gcagcacatc cggcgtggcg ctaaccagtt tcgcattgag
     6841 ttcagacttc cagcggtgcg cggtttccca actcacctgc tggccttcaa aaccgagcgt
     6901 ttgcaactgc tcatcggcaa tgatttgatt gacgatagtg gtaagcgtgt tcgtcgcgtc
     6961 aagcaaatgc agctggtagc cttgcgcacg ggcttccacc tcaacgaaat agcgcgaatc
     7021 taccagaatg tgcgcacttt cacggctaat aaccacatag cctgatccgg tggagatccc
     7081 cagatgcggc tgtttgttct gccgtgagga gagaagcact gcatccagtt gttgcgcctt
     7141 aagccagtcg cgcagcgaag cgagtaatgt catctggatt ttccttctta caggctatcg
     7201 attaacaatt tacctttacg gaacatcatc agacgcagga acaccaccat cagcgcagtt
     7261 atgactgccc ccagcgcgat ccccgccata tagacgccca ggttagtgac cagcggccat
     7321 gcccagatag cggattccgg gaaccattgc actgcgccca gccagacagc ggcggttgag
     7381 ccgacaatcg cgccgaccat atacgacgga atggcggtga tggggctttc cagcgcaaac
     7441 ggaatcgcac cctcactaat ccccataaag gcaaggaaca tcgcggtctt accctgcgga
     7501 taaagttgag cgttgaacag gcgtttgccg gttaaacgac ggtcgataat ggtcgccaga
     7561 cccagaccta tcggcggaat aacgatagcg atagaacgtg cagtgaccgg taatacgtgg
     7621 tcagtggtaa agctgaaggc aacaaaacct gcggctttgt tgatcgggcc accaaggtcg
     7681 attgcggtcg ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcggca
     7741 gtcagcacgg tacggatacc gccgttgatc cagccgccaa agggggtaat gacgtagtac
     7801 atcgccagca tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct
     7861 aacaggaaat ccggtaactg gattttctgg ttcatccact tcacaaggta acccgcgacg
     7921 atagaaataa tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc
     7981 atagtgctgg gatcgaagtt cagcagttgc gtcggctggg tagacatcaa gccgccgata
     8041 aaaccagccg ggaacgccag tttgccaccg atagagttcg ccacaaaagc agcaaacatc
     8101 ggaatggcaa aaccaaacaa cacgccgccg aaggactgcg acagccaggc aaatttcagt
     8161 aaggagaggt cgaaaccgga gaatttcccg ctattaagag catccatgat gccgatctca
     8221 gcaggaattt taagccagct ataagcaatc agctggctga aagcgaggat cacaccgccc
     8281 ataattaagg tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc
     8341 cagaagctgc ttttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca
     8401 ggcacaacgg ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac
     8461 tgttgttcag aggcaatcat ctcttcgatt tctttgatga tgcccgcagc gtttttaatt
     8521 gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga aacgctcatt atcttccggg
     8581 gtaactgcca cggagtggat gatgatggtt gcttcggcga tatcctgcgc cgtcaggcga
     8641 ttctggatac cgtccgcgcc ctgggtttca attttcactt cataacctgc ttctaccgcc
     8701 gcttcttcca gcgcctgagc ggccataaag gtgtgagcca ggcccattgg gcaggcacat
     8761 aaggcaatta gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa
     8821 ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta
     8881 atcccgctgt acggaagtgt gacgcaggtc ggccttgtga gggggacaca aagaaaaatt
     8941 gaattaactg tatgatttaa aagattatcg ggagagttac ctcccgatat aacagaaagg
     9001 atttacagaa tgtgccctaa ggtctggcgt aaatgtgcac cggaaccgag aagacccgga
     9061 ttgtcatgga cgatgagata caccggaata tcatggacat attctttaaa gcgcccttta
     9121 tcttcaaatg cggcacggaa accagaggct ttgaagaact caaggaagcg cggcacgata
     9181 ccgccagcaa tataaacccc accaaaggtt cccagcgtta atgccagatt gccgccaaaa
     9241 cggcccataa tgacgcaaaa cagcgacaac gcacggcggc aatcggtgca gctgtcagcc
     9301 agcgcgcgtt cggtaatatc ttttggcttg agattttctg gcaggcggtt gtcggcttta
     9361 acaattgcgc gatacaaatt caccagccca ggaccagaaa gcacgcgttc cgccgaaaca
     9421 tgaccaattt ccgcacgcaa tatttcgagg ataatggcct cttcttcact attcggcgca
     9481 aaatcaacgt gaccgccttc gcccggcaag cttacccaac gcttatcgac atggaccaga
     9541 tgcgcaaccc caagccctgt tccggcaccg taaaccgcaa taggtttacc ttcgaccggt
     9601 tctgcgccac caaactgaat cagatgctct tttttcagca tcgggatcgc catcgataca
     9661 gcggtaaaat cgttaataat ttccagatgg ctaaaaccga gattcttttt catttcggca
     9721 attgagaacg cccaggtatg gttggtcatt gccacccagt caccggtaat tgggcaagcg
     9781 atggcaatac agccgtcttt cacctcgacc ttatgttctt caagataaac gcgaatgacc
     9841 gcttcgaggc tggggtaatc aagccctgaa taggtcttag cctgcgagat ttcaccactg
     9901 gcaatatcac acagagcaag acgtgcgttg gtgccgccca catcaccgac taatgcatac
     9961 tttgtcattc ttcaactgct ccgctaaagt caaaataatt ctttctcaca ctgtaaatac
    10021 ctgggggcat aacaacaacg ccggaaaggc aggctccctg taaatatcga tctgggtcac
    10081 acatttactt tatcgtttca gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac
    10141 taagtatctg accccgcata aggaatagaa catgctccat ccgcgagcca gaaccatgtt
    10201 gttattatcg ctccccgccg tggcaattgg gattgcgtcc agtcttattc tgattgtggt
    10261 gatgaaaatc gcctcggtat tacagaattt gctctggcaa cgactgccgg gaactctggg
    10321 gatagcccag gattcaccta tctggatcat cggtgtatta acgctaacgg gtattgcggt
    10381 ggggttggtt atccgtttca gccagggtca tgccggacca gaccccgcct gtgaaccgct
    10441 gatcggcgca ccagttccgc cctctgcgct accaggactt atcgtagcat taattctcgg
    10501 tcttgctggc ggcgtcagcc tggggccgga acatccgatc atgaccgtca atatcgccct
    10561 tgccgtcgcc attggcgctc gtctgttacc gcgcgtcaac cgaatggagt ggactatttt
    10621 agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct gttgcggcgg cgttgatatt
    10681 ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg gatcgtcttt ttgcgccgtt
    10741 aatggcggca gcagctggtg cacttactac cggattattt ttccatcccc atttttcact
    10801 gcccattgcg cattacggac agatggaaat gaccgatatt ctcagcggtg caattgtcgc
    10861 ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc ttaccacggt tgcacgcgat
    10921 gatgaatcaa atgaaaaatc cggtgctcgt gctgggtatt ggcggattta ttctcggaat
    10981 tctgggggtt attggtggac cagtttcgct gtttaaaggg ctggatgaga tgcagcagat
    11041 ggtggcaaat caggctttca gcaccagcga ttactttttg ctggcggtaa ttaaacttgc
    11101 cgccctggtc gttgctgccg ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt
    11161 tgtcggcgtg gcattagggt tgatgctgca tgagcacgtt cccgccgttc cagcggcaat
    11221 aaccgtttcc tgcgctattc tcggcatcgt gctggtggta acacgcgatg gctggttaag
    11281 tctttttatg gcggcagtcg ttgtacccaa taccacattg ctaccgctgc tctgtatcgt
    11341 catgcttccg gcatggctgt tattagcagg taagccgatg atgatggtca atcgtccgaa
    11401 gcaacagcca ccccacgata atgtttagca aaaaatgctc tcctttatga tttaagagtt
    11461 atggcgatga tacggggctt tacctccccg taatattgcg ttaacaggcc gctgacggct
    11521 tcgatcgtga aggagaataa caatgttcag gtcactgttt ctggcggccg ccctgatggc
    11581 atttaccccg cttgcagcaa atgcaggtga aatcacccta ctgccatcga tcaaattaca
    11641 aattggcgat cgcgatcatt acggtaatta ctgggacggt ggtcactggc gagatcgtga
    11701 ttactggcat cgcaattatg aatggcgcaa aaaccgctgg tggcgtcatg ataatggcta
    11761 tcatcgtggc tgggataagc gtaaagcata tgagcgtggc tatcgtgaag gctggcgcga
    11821 tcgtgacgat catcgcggaa aaggccgcgg acatgggcac cgccattaaa cgcgtcctcc
    11881 aatggagcac aatgcctgat gcgacgctag cgcgtcttat caggcctaca aaccggctct
    11941 ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc caatagtgcc agatgcgacg
    12001 ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca gcgccacgac
    12061 cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat
    12121 caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga taccaaaact
    12181 taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca gaatgacaat
    12241 aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga agcgaatgaa
    12301 tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca gtccggcagc
    12361 aaccagactt aacccaaaga ccgttgccgc agcatggctt aacagcggtt gcagcgtcag
    12421 ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg ccgccgcagc
    12481 tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc atggcgatag ccacatccca
    12541 tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag tgagcgaaga
    12601 gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg ccaggaagac
    12661 cgcctccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg ccaggttagg
    12721 ctgggagaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta acccgccaat
    12781 cactttctcc agtggttttt gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat
    12841 ccccgtcagc accgcgccct gcaacaacga gacaccaaga atgagtttaa aaccgatcgc
    12901 cgcaccaata aattccgcca ggtcggttgc catcgcaata atttctgcct gaacccaata
    12961 gaaccacact acgggacgcg gatagtgatc acgaatctgc tccgccagat tttttccggt
    13021 ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt tggcccaaac
    13081 gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg tcgcaaagtt
    13141 accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg cgagcctcat
    13201 cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct tgtgcctcta
    13261 aaacatagcc tttgctatgt ttcatgctat gataaacgag aatgattatc aaattcattt
    13321 aaatggattg tggtgatttc tctgatagac caggattatg actacgaaaa gattgatgac
    13381 ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc ttccatacac
    13441 aacatagcag aaatgtatga cagatcacta tttttgaagc ctgtcacagg acgtcattat
    13501 agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac ggaaatttaa
    13561 cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg gcacttgccg
    13621 ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg
    13681 ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac tccgacgttg
    13741 gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcaga tttgccaacg
    13801 aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc ttcttcctga
    13861 aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag cacattcgcg
    13921 tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac ggtatgggca
    13981 aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtctgaa aactttattg
    14041 cctataaaga tatcctcggc aaaatctccc gtaatcgtat gtacaccatg gctgccacgg
    14101 caatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg gaaccgaaat
    14161 acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca
    14221 atccttaccg tgttgatgcc agtgaagaaa acatccagat gtccaacctg cacgaaggtc
    14281 agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaagtt gccattatcg
    14341 ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca ctgtttgcca
    14401 ccgttactgg ctggtttggc tacagcatct ccttccaggg catcctgggc tacatcttct
    14461 atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca
    14521 tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag aaaattgctt
    14581 ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc ttcgctaact
    14641 tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg
    14701 tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt gtgctgtctg
    14761 cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga tcattccccg
    14821 gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat acccttgcag
    14881 atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga caaactccgc
    14941 gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa tcatcgcatc
    15001 cagcgtattc gtgacaatat ctttcacaaa gacgccatca attttgatga tatcagcctg
    15061 caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa tcgcaatccg
    15121 gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt tagaaaacgc
    15181 ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac gcttaaacag
    15241 acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta aattgactga
    15301 aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca aggattccag
    15361 cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca gaaacttatc
    15421 cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat aaccttcacc
    15481 ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta acgccgtgcg
    15541 aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct cctctctgct
    15601 atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc ttaactgccc
    15661 caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag caccatagcc
    15721 catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt taaccatatg
    15781 ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac tacccggcaa
    15841 ttgatataac ttttcgtttt cctgcatcaa cggcagcagc gtacggtaaa ttgatcggat
    15901 acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat tatcaatgcg
    15961 caggcagcaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc acggaagttg
    16021 ggcaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcgatt aagccattca
    16081 ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat cagcaccgca
    16141 agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg gttgtaattc
    16201 agaaggcaaa gcgttgaaac agccctggtg agatttaaaa acggatagcg aagcttaccg
    16261 accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat aaagtcgttt
    16321 tcataaggtg tgcacaacag aagtagaagc acgcttaacg ctgccagcca gcttaaggta
    16381 aatgcgcgtt tctctttgcc caacgacggc gcgatatccc tgcgccacaa tatctgcgca
    16441 aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta aataagcaca
    16501 gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag
    16561 gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg
    16621 ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat
    16681 ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca
    16741 aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga
    16801 ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag
    16861 attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg
    16921 gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt
    16981 gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca
    17041 ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg
    17101 ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca ctaacccgga
    17161 cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc tttgcagcga
    17221 gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc atgccatgca
    17281 agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat ttgctgggtg
    17341 caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc
    17401 tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg gcggcatcat
    17461 atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa tcgctgaaaa
    17521 atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc atgtcacggt
    17581 ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat tcagggaatt
    17641 gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg ctatagccgc
    17701 cagaccatta ataaatgggt acgcaaggag ggctggacaa cgtcaccaaa acctggcgtc
    17761 cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata tattcgcaat
    17821 gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc gccgcttgaa
    17881 gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca gtttacatcc
    17941 ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg cgatagcaaa
    18001 taatatgaaa agattacgca ataaaatgac caccgaagaa ctggctgaat gcctcggtgt
    18061 ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg aaaaatttcc
    18121 cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt gcgagtttat
    18181 tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag agagaattgc
    18241 tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg cgatagataa
    18301 tatgacagac attgagcagg gaaaagtcgc gcaatttttg tcacgtgaag gaattcgcaa
    18361 tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca ggatattatc
    18421 tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca gcaataaaat
    18481 ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga acggtaacat
    18541 ccagtgctgg agactgcccc gccccggtta cggcgacacg cagcggcata ccaactttac
    18601 ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg ttttcagcgg
    18661 tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga cgcgctaccg
    18721 gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg tagaaataac
    18781 ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt ttcaccagat
    18841 cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc cactgtaagt
    18901 gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc cacagcagct
    18961 tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag tatttgatca
    19021 tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc accagatagt
    19081 tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg actgccccgt
    19141 gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc gcgtaaaccg
    19201 gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg ttgatatggt
    19261 cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc acacagaagt
    19321 tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc tggttgctga
    19381 actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc gggttagcaa
    19441 aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg cagcgaccgt
    19501 cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt
    19561 tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc accgcgttgt
    19621 agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg ctcagccagt
    19681 tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt
    19741 caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag taaagagcag
    19801 tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga gttttgattt
    19861 tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga
    19921 gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt tctaccttac
    19981 gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc atcttgctta
    20041 aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg aactctccct
    20101 ataatgcggc tccacacagc gggggtgatt agctcagctg ggagagcacc tcccttacaa
    20161 ggagggggtc ggcggttcga tcccgtcatc acccaccaac atactttatg tagtctccgc
    20221 cgtgtagcaa gaaattgaga agtgggtgat tagctcagct gggagagcac ctcccttaca
    20281 aggagggggt cggcggttcg atcccgtcat cacccaccaa tttctcgcca gctaaatttc
    20341 ttgtaaaaat gtgaagtacc gaagtgggtg attagctcag ctgggagagc acctccctta
    20401 caaggagggg gtcggcggtt cgatcccgtc atcacccacc actt
//
LOCUS       DAJTXQ010000075        20231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000075 DAJTXQ010000000
VERSION     DAJTXQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.075"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            690..1460
                     /locus_tag="OO515_002556"
     CDS             690..1460
                     /locus_tag="OO515_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6576458.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="OO515_002557"
     CDS             1451..1924
                     /locus_tag="OO515_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6576459.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="OO515_002558"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="OO515_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6576460.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="OO515_002559"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="OO515_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6576461.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="OO515_002560"
     CDS             3360..3854
                     /locus_tag="OO515_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6576462.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="OO515_002561"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="OO515_002561"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6576463.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OO515_002562"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OO515_002562"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6576464.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="OO515_002563"
     CDS             7699..8613
                     /locus_tag="OO515_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6576465.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10179
                     /locus_tag="OO515_002564"
                     /pseudo
     CDS             8828..10179
                     /locus_tag="OO515_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OO515_002565"
                     /pseudo
     CDS             complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OO515_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OO515_002566"
     CDS             complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OO515_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6576466.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12720..14009
                     /locus_tag="OO515_002567"
     CDS             12720..14009
                     /locus_tag="OO515_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6576467.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14006..15472
                     /gene="hpaE"
                     /locus_tag="OO515_002568"
     CDS             14006..15472
                     /gene="hpaE"
                     /locus_tag="OO515_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6576468.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15474..16325
                     /gene="hpaD"
                     /locus_tag="OO515_002569"
     CDS             15474..16325
                     /gene="hpaD"
                     /locus_tag="OO515_002569"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6576469.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16335..16715
                     /locus_tag="OO515_002570"
     CDS             16335..16715
                     /locus_tag="OO515_002570"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6576470.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16826..17629
                     /gene="hpaH"
                     /locus_tag="OO515_002571"
     CDS             16826..17629
                     /gene="hpaH"
                     /locus_tag="OO515_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6576471.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17640..18428
                     /gene="hpaI"
                     /locus_tag="OO515_002572"
     CDS             17640..18428
                     /gene="hpaI"
                     /locus_tag="OO515_002572"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6576472.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18603..19979
                     /gene="hpaX"
                     /locus_tag="OO515_002573"
     CDS             18603..19979
                     /gene="hpaX"
                     /locus_tag="OO515_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6576473.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19989..>20231
                     /locus_tag="OO515_002574"
     CDS             19989..>20231
                     /locus_tag="OO515_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6576474.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4764 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcaacat cctgctgggg cttgatccac
     9961 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
    10021 atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc
    10081 cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc
    10141 ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc ccgataacat
    10201 tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct cgctatctgc
    10261 cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg atgtttcacg
    10321 ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg cctgctcttc
    10381 cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt gagttacccg
    10441 gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc
    10501 agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa tctccgccat
    10561 tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc
    10621 aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat tacgcacttc
    10681 tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc
    10741 gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc
    10801 gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt
    10861 gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt
    10921 ttcggcgttc tgcttcaccg ttgcggtcag ttactccatg ctggctgccg tctcttccag
    10981 cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct
    11041 ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc
    11101 tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc
    11161 aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat
    11221 tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac
    11281 gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac
    11341 ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc
    11401 gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag
    11461 ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc
    11521 tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc
    11581 cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc
    11641 aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg
    11701 caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac
    11761 agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg
    11821 taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa
    11881 catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta
    11941 tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag atactaaaaa
    12001 gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca ataaattcct
    12061 ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat
    12121 aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg
    12181 agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt
    12241 ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat
    12301 cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag
    12361 tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga
    12421 gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa
    12481 tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat tcctgatttt
    12541 atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta tctttaaatg
    12601 taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac tacgcaatcg
    12661 ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga ggagtggtaa
    12721 tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat gcatggcagg
    12781 aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac
    12841 cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaggtac
    12901 tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag
    12961 atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga
    13021 gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa
    13081 ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac gggcgtcctg
    13141 ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg agcgccctga
    13201 gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca caggcgcgcg
    13261 tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa
    13321 atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg cagccacacc
    13381 cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat
    13441 ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc actggcgata
    13501 accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg gagctggtgg
    13561 tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg
    13621 gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac taccgcccta
    13681 acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag
    13741 aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc
    13801 agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagtg
    13861 aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa ggcttatctg
    13921 acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc
    13981 gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa
    14041 tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag tgctggcgga
    14101 tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt
    14161 cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga
    14221 tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca ccggcctgcc
    14281 gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg aatttttcgc
    14341 ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta
    14401 cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg taccgtttat
    14461 gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat
    14521 gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg aagccgatat
    14581 tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg atgcgctggt
    14641 tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg ggcgcaacat
    14701 catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat cgccggtgct
    14761 gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca ccatcttctc
    14821 gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa gcatctaccc
    14881 ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga
    14941 tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag tctccggcta
    15001 tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc
    15061 cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt
    15121 tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc
    15181 gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg gcctcgcgtc
    15241 gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagctgg
    15301 catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg gcggcgtaaa
    15361 agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa
    15421 gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta
    15481 agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct gaactgccag
    15541 ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc agcaagcgtt
    15601 gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg gtcaacagtg
    15661 cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc
    15721 attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg cagcttattg
    15781 ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc agcctgaaac
    15841 tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac ttcaaagtgg
    15901 tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag ctgggcgaag
    15961 cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt
    16021 tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg
    16081 agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc cagttcaaag
    16141 agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg
    16201 acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag gtggagttta
    16261 tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg
    16321 cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc cgcgaagaag
    16381 ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg ggtatttttc
    16441 cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg gccgacgggc
    16501 agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc agcctggaaa
    16561 gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc gccgccctga
    16621 tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg acgctgaatt
    16681 ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg gtggcgctgc
    16741 gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg cgcagccgca
    16801 tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca
    16861 gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc
    16921 ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc tgaaaatcgc
    16981 cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggcaag
    17041 ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct tccacgatgg
    17101 cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt
    17161 gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga
    17221 ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac
    17281 ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg ccggggtgat
    17341 cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat
    17401 gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga atcatccggc
    17461 aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg
    17521 gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt
    17581 ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag gagagaacga
    17641 tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga ttatggctgg
    17701 ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac tggttgttga
    17761 tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg
    17821 cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac
    17881 aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag
    17941 cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg
    18001 cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc
    18061 aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc
    18121 tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg
    18181 gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc
    18241 agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac
    18301 gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc
    18361 gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg aagcccggcg
    18421 tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa ccgtaggccg
    18481 gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac cggataaggc
    18541 gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt agaggaagaa
    18601 aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat cagcataaag
    18661 cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg agcgtctttc
    18721 tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt gccggactca
    18781 cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc accctgttct
    18841 atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt gtcggtgcgc
    18901 ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc accatgtttg
    18961 ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc gaagccggct
    19021 ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc cgcgcccgtg
    19081 ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg atcgtttccg
    19141 gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg ctgtttttgc
    19201 tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt gatgactcac
    19261 cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag atgatggata
    19321 acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc atgcaacaac
    19381 gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg gcgtatttct
    19441 gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg caaagtttta
    19501 atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag atttgtacca
    19561 ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga aggcatcaca
    19621 ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca actgatcaca
    19681 acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc gcaatggcga
    19741 ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc ggtattgcgg
    19801 tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc ggctggttga
    19861 aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg ctggtgattg
    19921 gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg accctgtaag
    19981 gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag tacgatgaaa
    20041 gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca ttttttggcc
    20101 gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc aatagcggac
    20161 agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg tttgtcctga
    20221 cgccgccgtc a
//
LOCUS       DAJTXQ010000076        20144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000076 DAJTXQ010000000
VERSION     DAJTXQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.076"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1274)
                     /locus_tag="OO515_002575"
     CDS             complement(<1..1274)
                     /locus_tag="OO515_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576475.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHA"
     gene            complement(1467..1536)
                     /locus_tag="OO515_002576"
     tRNA            complement(1467..1536)
                     /locus_tag="OO515_002576"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1502..1504),aa:Gly,seq:ccc)
     gene            complement(1615..2370)
                     /locus_tag="OO515_002577"
     CDS             complement(1615..2370)
                     /locus_tag="OO515_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6576476.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2785..5082
                     /gene="xdhA"
                     /locus_tag="OO515_002578"
     CDS             2785..5082
                     /gene="xdhA"
                     /locus_tag="OO515_002578"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6576477.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5093..5971
                     /gene="xdhB"
                     /locus_tag="OO515_002579"
     CDS             5093..5971
                     /gene="xdhB"
                     /locus_tag="OO515_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6576478.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5968..6447
                     /gene="xdhC"
                     /locus_tag="OO515_002580"
     CDS             5968..6447
                     /gene="xdhC"
                     /locus_tag="OO515_002580"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6576479.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6487..8265)
                     /locus_tag="OO515_002581"
     CDS             complement(6487..8265)
                     /locus_tag="OO515_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6576480.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8744..9931
                     /gene="ygeW"
                     /locus_tag="OO515_002582"
     CDS             8744..9931
                     /gene="ygeW"
                     /locus_tag="OO515_002582"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6576481.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9989..11185
                     /gene="dpaL"
                     /locus_tag="OO515_002583"
     CDS             9989..11185
                     /gene="dpaL"
                     /locus_tag="OO515_002583"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6576482.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            11243..12454
                     /locus_tag="OO515_002584"
     CDS             11243..12454
                     /locus_tag="OO515_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6576483.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12507..13891
                     /gene="hyuA"
                     /locus_tag="OO515_002585"
                     /pseudo
     CDS             12507..13891
                     /gene="hyuA"
                     /locus_tag="OO515_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13939..14871
                     /gene="arcC"
                     /locus_tag="OO515_002586"
     CDS             13939..14871
                     /gene="arcC"
                     /locus_tag="OO515_002586"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6576484.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14912..16537)
                     /locus_tag="OO515_002587"
     CDS             complement(14912..16537)
                     /locus_tag="OO515_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6576485.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16585..17355)
                     /gene="yqeC"
                     /locus_tag="OO515_002588"
     CDS             complement(16585..17355)
                     /gene="yqeC"
                     /locus_tag="OO515_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6576486.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17458..18036
                     /gene="mocA"
                     /locus_tag="OO515_002589"
     CDS             17458..18036
                     /gene="mocA"
                     /locus_tag="OO515_002589"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6576487.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18358..>20144
                     /gene="ygfK"
                     /locus_tag="OO515_002590"
     CDS             18358..>20144
                     /gene="ygfK"
                     /locus_tag="OO515_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR6576488.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5225 a         5179 c         4764 g         4976 t
ORIGIN      
        1 ttcgcatgtg aattttgatc ggtatcggtt ttaatatcat tgaaccaaaa atttgccgac
       61 tgataattat ccttctcgta tctttttata ccatacggac ctgctagttt tttaactatt
      121 tttagaacct gctcttttct tctggtgttt atctttgcaa gatttgcagg ataaataaca
      181 ttaagtaaag ccgcatcggc ttctctgtaa tgtttatctt tttccagata acctggggat
      241 tctcccccta aatcaagctg taacgttatt ctttcataac ctttatctat gaggtggttc
      301 aacctcgttg ttgaaagagg ttcatccagt tcattggctt tagcttcgcg tagtaaatca
      361 gagacaaaaa cagagtcttt cttcgagagt aagttcgaca aacgttccag tccagaggtc
      421 accagtgcaa cggaagatgt gtttaatcgg gcatcttctt cccatgctcc ggaatcttcc
      481 ataacataga aattggcttt atccagataa gcaatcagca gcgcaaccga ttttaatcgg
      541 tcaccttttt gccaatcttc agcgttaatt gtaccagtgt cgattgcttg tataaggaga
      601 tcaagataaa gacctaacgc atcattttgt ttatggttcc acagctgcgg cttgccttct
      661 tcctgcacat ctgccatcac gggggagttg ctgtcaaatc ggatatgcac agcattcatt
      721 tgcccgggaa ctccatccag tcgtttgggg ttgctaatga cgtcctgcat ccgcttaatc
      781 tggtcgagcg tagacatata atcccacagg gtgagcagaa ccttttttgc ggctacacta
      841 tttccctgat ctgatactaa tgccatatag ccccataaac tatccctcag ccagacagca
      901 tcatagtggg tttctgccgc tgttttgtca tcttcgaaag tagaagcaag aataaatgtt
      961 ccccagggtt tctccgtcaa cgttaacttt cgtgtgtttt ctgttaatgt taacaaccct
     1021 tgaatttcgt ctttggtata gtggctcttt atttcatttt ttaatttggc tgagtgaatc
     1081 agaagacgat aattagggta cttatctgga tttaacgata aataactttt atctgttgct
     1141 gcccccgtgg aattaagaat gttaaataca gattcactcg tcacttctgc ccctgtcgcc
     1201 tggcatgtgc cgcacgaaaa tataccagcg agcagaagag ttaatgcaga tttccctttc
     1261 ataaaatatt ccatcaatcg tttgttaaaa gcgtaaataa ccacttattt aacaacttga
     1321 aaataaggtg ttttacctgg gttgttacaa aaggattgca ttgcgtaaac gctttttatt
     1381 tacaacaaaa tggggaaata ttacggcgat tgcgaaaagt ctgaagcgat tgaattaaga
     1441 taatatagaa aatcagatac agcaaaagcg gcgaagggaa tcgaaccctc gtatagagct
     1501 tgggaagctc tcgttctacc attgaactac gcccgcttcg agatgcgtaa ggcattataa
     1561 accttacgct ctccttagca agtgccgcgc tgctgactgc tgattaattc gccatcagca
     1621 ttttggcttg ctgccctgcg gcggcaagta acgtagcgga tcaattgccg ttgcacggta
     1681 acgaatctgg aaatgcaggc gaacagatgc cgcatccgtg ctccccatag tggcgatttt
     1741 ttgcccagcc ttcacgcttt gcccattatt taccagcatc gtgtcattat gggcgtaagc
     1801 cgtaatgtaa tcttcactgt gtttaatcat gatgagatta ccgtagccac gcagctggtt
     1861 gcccacatac accacctttc ctgcacccgc ggcgtaaata ggtgtacccc gtggagctga
     1921 gatatcaatc cctttattgc cgccatctgc tgtcgaatac ggcatgataa ctttccctgt
     1981 cgttggccat aaccaacaac gttgccctac tggcggccag gatgatttcg gtaccgctga
     2041 tgacggtgta accgatgcgg ttttggtcgt tgatttggcg gttgatttac gtgtactact
     2101 gctacttttc gccccaccca gttttagttt ctgaccaact tcaatggtgt aaggggggga
     2161 aatgccgttc agtcgcgcca gttcttttac gctggttccc gtggtgcgcg aaatacgata
     2221 tagcgtatcc ccccgtttca cggtgtaaac ggagccggaa tacgttcctg tatcggatga
     2281 tttgctaccc gaacagcccg ccaaaagcag tccaaccgat aacaacatca cgatacccag
     2341 agatttttta ttcaggcgtc ccgcactcaa aatctttcct cgctaaaaaa acaagacgcc
     2401 ttatcatagc agccagcctc ttgataccca atctcttgct tctggaaaaa cgtggtaata
     2461 gagaaagagg ttaatttcat ttggcaaagg gaagtatcat cagcaaaata ttcgctgtag
     2521 aggtatcgaa cttcctgact ttttacccta tttcccatcc tgatgcgtaa taatcccaat
     2581 ttatcacatt gattttatcg ccactcatat tgatacgtat cactgcccct attcagagtc
     2641 ctgtttcgta tcatatctgt gtttttttaa cgtcatcaca cttcctacct cccctaacgc
     2701 ttatcgtcgt ttctggcgta aatcttgcct gcttagacta aatctttgcc ataagaacca
     2761 aatgttcaaa tcgaggggat ttctatggaa gcgcgggaag caaccgctac gggtgaatca
     2821 tgcatgcgcg tcgatgccat tgctaaggtc accgggcggg cacgatatac tgacgattat
     2881 gttatggcgg gcatgtgtta tgcgaaatat gtacgtagcc ctatcgcaca tggttatgcc
     2941 gtaagtatta atgatgaaca agccagaagt ttgccgggcg tactggcgat ttttacctgg
     3001 gaagatgtgc ctgatattcc attcgctaca gctgggcatg cctggacact tgacgaaaac
     3061 aagcgcgata ccgccgatcg cgcactgcta actcgccatg ttcgtcatca tggtgacgcc
     3121 gttgccatcg tcgtggcccg cgatgaactc acggcagaaa aagcggcgca attggtcagc
     3181 attgagtggc aagaattacc cgttatcacc acgccagaag cggcgctggc agaagacgct
     3241 gcaccaatcc ataacggtgg caatttactg aaacaaagca cgatgtcgac gggtaatgtc
     3301 caacaaacaa tcgatgccgc cgactaccag gtacaggggc actatcagac ccccgttatt
     3361 caacattgtc acatggaaag cgtaacatcg ctggcgtgga tggaggatga ctcgcgaatt
     3421 accatcgttt ccagcaccca gatcccgcac attgttcgcc gcgtggttgg tcaggcgctg
     3481 gatattccct ggtcatgcgt acgagtcatc aaaccgtttg tcggtggcgg ttttggtaat
     3541 aaacaggatg tactggaaga gccaatggca gcattcctga ccagcaagct tggcggcatt
     3601 ccggtgaaag tttcccttag ccgtgaagag tgtttcctcg caacccgtac ccgccacgct
     3661 tttaccattg acgggcaaat gggcgtgaac cgcgacggaa cattgaaagg ttatagtctg
     3721 gatgttctgt ctaacaccgg cgcttatgca tctcacgggc actccatcgc ttctgcgggg
     3781 aggaataaag tcgcttacct ttatcctcgt tgtgcctacg cttacagttc aaagacctgc
     3841 tataccaacc tcccctcggc tggtgcgatg cgtggttatg gcgcgccaca agtcgtattt
     3901 gccgttgagt ctatgcttga tgacgccgcg acagcgttag gtattgatcc tgttgaaatt
     3961 cgtttacgca acgccgcccg cgaaggagat gctaatccgc tcacgggcaa acgtatttac
     4021 agcgcagggt tgccggagtg tcttgaaaaa ggccggaaaa tctttgaatg ggaaaaacgc
     4081 cgtgcagaat gccagaacca gcaaggcaat ttgcgccgcg gcgttggcgt cgcctgtttt
     4141 agctatacct ctaacacctg gcctgtcggc gtagaaatag caggcgcgcg ccttctgatg
     4201 aatcaggatg gaaccatcaa cgtgcaaagc ggcgcgacgg aaatcggtca gggtgccgac
     4261 accgtcttct cgcaaatggt ggcagaaacc gtgggggttc cggtcagcga cgttcgcgtt
     4321 atttcaactc aagataccga cgttacgccg ttcgctcccg gcgcatttgc ctcacgccag
     4381 agctatgttg ccgcgcctgc gctgcgcagt gcggcactat tattaaaaga gaaaatcatc
     4441 gctcacgccg cagtcatgct acatcagtca gcgatgaatc tgaccctgat aaaaggccat
     4501 atcgtgctgg ttgaacgacc ggaagagccg ttaatgtcgt taaaagattt ggcgatggac
     4561 gctttctacc accctgaacg cggcgggcag ctctctgctg aaagctccat caaaaccacc
     4621 actaacccat cagcgtttgg ctgtaccttt gttgatctga cggtcgatat tgcgctgtgc
     4681 aaagtcacca tcaaccgcat cctcaacgtt catgattcag ggcatattct taatccactg
     4741 ctggcagaag gtcaggtaca cggcggaatg ggaatgggca ttggctgggc gctatttgaa
     4801 gagatgatca tcgatgctaa aagcggcgtg gtccgtaacc ccaatctgct ggattacaaa
     4861 atgccgacca tgccggatct gccacaactg gaaagcgcgt tcgtcgaaat caatgagccg
     4921 caatccgcat acggacataa gtcactgggt gagccaccaa taattcctgt tgccgctgct
     4981 attcgtaacg cggtgaagat ggctaccggt gttgcaatca atacactgcc gctgacgcca
     5041 aaacggttat atgaagagtt ccatctggca ggattgattt gaggataaca tcatgtttga
     5101 ttttgcttct taccatcgcg cagcaaccct tgccgatgcc atcaacctgc tggctgacaa
     5161 cccgcaggcc aaactgctcg ccgatggcac tgacgtactg attcagctcc accatcacaa
     5221 tgaccgttat cgccatattg ttgatattca taatctggcg gagctgcggg gaattacgct
     5281 ggcggaagat ggctcgctac gtatcggctc tgcaacgaca tttacccagc taatagaagc
     5341 tcctataact caacgtcatc tcccggcgtt atgtgctgcg gccacgtcca ttgctggacc
     5401 gcagatccgt aacgtcgcta cctacggtgg aaatatttgc aacggtgcca ccagcgcaga
     5461 ttctgccacg ccaacgctaa tttatgacgc gaaactggag atccactccc cgcgcggtgt
     5521 tcgtttcgtc ccgattaatg gctttcacac cgggccgggc aaagtgtctc ttgagcatga
     5581 cgaaatcctc gtcgcctttc attttccgcc acagccgaaa gaacacgcgg gcagcgcgca
     5641 ttttaaatat gccatgcgcg acgcaatgga tatttcaacg attggcagcg ccgcacattg
     5701 ccgactggat aacggcaatt tcagcgaatt acgcctggca tttggtgttg ccgcgccaac
     5761 gccgattcgc tgccaacatg ccgaacagac tgcacaaaat gcgccattaa acctgcaaac
     5821 gctggaagct atcagcgaat ctgtcctgca agatgtcgcc ccgcgttctt catggcgggc
     5881 cagtaaagag tttcgtctgc atctcatcca gacgatgacc aaaaaagtga ttagcgaagc
     5941 cgtcgccgcg gcggggggaa aattgcaatg aatcacagcg aaacaattac catcgaatgc
     6001 accattaacg ggatgccttt tcagcttcac gccgcaccag gcacgccgct ctcggaatta
     6061 ctccgcgaac aaggactgct aagtgtcaaa caagggtgct gcgtgggtga atgtggtgcc
     6121 tgtacggtgt tggtcgacgg cacagcaata gacagttgct tataccttgc cgcctgggct
     6181 gaaggaaagg agatccgcac gctggaaggt gaagcgaaag gcggaaaact ttctcatgtt
     6241 cagcaggctt atgcgaaatc cggcgcagtg cagtgcgggt tttgtacgcc tggcctgatt
     6301 atggctacca cggcaatgct ggcgaaacca cgcgagaagc cattaaccat tacggaaatt
     6361 cgtcgcggac tggcgggaaa tctttgtcgc tgcacggggt atcagatgat tgtaaataca
     6421 gttctggatt gcgagaaaac gaagtaaaag gatatccggc ctgaattcag gccggattca
     6481 ctgaggttat gtgtttaaca actcatattt cttaatcttg cgatagagcg tagcaatgcc
     6541 gatgcccagt tcatcagcaa cttgcttctt gctgttatga cgtgaaagcg cctcgcggat
     6601 catttgcttt tccatctcct ccagcgccgt gccgcccgca tcatcgagtg acaggtgcgc
     6661 ctcactgacc tctgttacat cactttgctc cgttgtgcca ttattcagca gatttggcgg
     6721 caatagcgtg ctgtcgataa cttcacctga aggaaccacg ttaaccagat attccatcaa
     6781 attgcttaac tcgcgcaggt ttccgggcca acgatgctta cgcaatattt cgacgacatc
     6841 gggagcaatg ccaggataaa ccgatcccag acgacgggta tgcagatgta aaaagtaatg
     6901 caccaatagt tcaatatctt cctgacgttc acgcagcggt ggcagagtta tcgggataac
     6961 attaagtcgg tagaagagat cttcgcggaa tttaccttcg gcaatgaact gggccaaatt
     7021 ctgattagtt gcagaaatga tgcgaatgtc gacttgtatt gggctactgg caccaatcgg
     7081 cagaatttca cgcgcctcaa tagcgcgcag taatttagcc tgcaacatta atggcatatc
     7141 acctatttca tcgagaaaca gcgtgcccgt attcgccgcc tgaatcaacc ctgttttacc
     7201 gttggcagaa gcaccagtaa atgcgccttt aacataaccg aacagttcgc tttccagaag
     7261 ctgctccgga atcgcggcac agttgatagc aataaagggt ttattccgtc ttccgctcaa
     7321 cttatggatt gctcgggcga cgacttcttt acccgtgccg ctttcaccaa ccaccataac
     7381 gctggatggg ctgggtgcaa tacggctaat gagtcgtttt aattgccgca taacacggca
     7441 ctcgccaacc aattgttcaa tatgtggttc atcaggtgca tttgctacag aaaaactggt
     7501 atgcgattgg tgaaatgcca ttaaaaataa ttgtcggccc tgaatgttat gcaattgacc
     7561 aatgattaat tcacttttat cgtcccacga aacaatatgc tgcatatgtc catgagtaaa
     7621 attactctca aatgttaatg gtctgaaacg gataggtttc ccaataatat tattttgtac
     7681 aacaccaagt gtttttaagg cagtctgatt aacaaactga acccgacttt catcatcaac
     7741 aactaatacg ccctgatcca tattatcgat catggtcgcg aatattttac tgatgttatc
     7801 tcctggcccc tgatcctcca gaagtttcga aacaaaaatg gtggatatat ggcgaacata
     7861 atcagaaaat tcgcgtaaat tatcactgat atgctcttgt tgctcgtggg taacggcaat
     7921 caaacttatc accccaacac aacgatcctg taaaatgaca ggcgtaccca gaaatgcttt
     7981 ttcgcggcaa ttttctttac tatcgcaacc ttcgcaaagg ggatcgaagc gagactgtgt
     8041 cacaactttt tcagttttcg tttccaggac gtggcggagc aggcgtgagt tgccgctcaa
     8101 ctggcgacca agaaacttcc catacgcgcc cgttccggcg acgcgacaca agttttcatc
     8161 aacgatctca acctcaaact gcaaaacgct ggcaagcatt ctggcaaaac gctgaattgt
     8221 cggttgaatt tgcatcaata ctgactgcgt agtagcaagc tccatagctt taccttccag
     8281 acttacttaa aagtcgatca ttgaagacgt tgatggttca cagatcatga tgatattaac
     8341 tcaggcgaaa ttggctttga taaaaacata agatttttat cattttctaa tgcaattatg
     8401 gaagagatat cacatttcta tatcaatatg agaattacgg cggtgagttt atcaaactga
     8461 agagagatag cctgcccctt tatcttattt ctgatactta gcagaaaata aataacgcga
     8521 taaaaaaagc caaacgtttt cgtattttac aaacaaccag aagctggcat caatttgtga
     8581 acaaccccac acattatccg tcaaattagt cttttgcagc cgcgcggata attctggcac
     8641 acttattgtt agtcccaggt atagctatga aaacaccaat cactttggca agtcacagtg
     8701 aaataaacca ctttgcctgt cattccacta ccgggactgt atgatgaaaa ctgttaatga
     8761 gctgattaag gatatcaatt cgctgacctc tcaccttcac gagaaagatt ttttgttaac
     8821 gtgggaacag acgccagatg aactgaaaca agtactggac gttgccgcag cattaaaagc
     8881 actgcgtgct gaaaacatct caaccaaagt ctttaatagt ggattaggta tttccgtatt
     8941 ccgcgacaac tccacccgta cccgcttctc ttatgcttcc gcgcttaacc tgctcggcct
     9001 tgcacaacaa gatctcgacg aaggcaaatc acaaatcgct cacggcgaaa ccgtgcgtga
     9061 aaccgccaat atgatctcct tctgcgccga cgctattggt attcgcgacg atatgtatct
     9121 gggcgcaggc aacgcctata tgcgtgaagt tggcgctgca cttgatgacg gttacaagca
     9181 gggtgtactg ccacagcgtc cggctttagt gaacctgcaa tgcgatattg accacccgac
     9241 tcagtcaatg gctgacctcg cgtggttacg tgaacacttt ggttcactgg aaaacctgaa
     9301 aggtaaaaaa atcgccatga cctgggccta ctctccaagc tatggcaaac cgctctctgt
     9361 accacaaggc atcatcggtc tgatgactcg cttcggtatg gatgtcacct tggcccatcc
     9421 ggaaggctac gacctgatcc cggatgtggt tgaagtggcg aaaaacaatg ctaaagcctc
     9481 cggtggtagc ttccgtcagg tcaccagcat ggaagaagcc ttcaaagacg cagacatcgt
     9541 ttatccgaag tcatgggcac cttacaaagt gatggaagag cgtactgaat tgctgcgtgc
     9601 gaacgaccac gaaggcttaa aagcactgga aaaacagtgt ctggcacaga acgcgcaaca
     9661 caaagactgg cattgtactg aagagatgat ggaactgacc cgtgatggcg aagccctgta
     9721 catgcactgc ctgccagctg atatcagcgg cgtatcctgc aaagaaggtg aagtgactga
     9781 aggcgtattc gaaaaatacc gtatcgctac ctacaaagaa gccagctgga agccttatat
     9841 catcgccgcg atgatcctgt cccgtaaata cgccaaacca ggtgcactgc tcgagcaact
     9901 gctgaaagaa gcgcaagaac gcgtgaaata acccctcggg ccgatctgct ggtcggccct
     9961 ctatttccag aggccaaaag gataggatat gtccgttttc tcattgaaga ttgatatcgc
    10021 cgataacaaa tttttcaacg gcgaaacatc accgctcttt tcgcaaagcc aggccaaact
    10081 ggcgcgccag ttccaccaga aaatagctgg ttatcgccca acaccgcttt gtgcgctgga
    10141 cgatctcgca aacctttttg gtgtgaagaa aattctcgtc aaagacgaat caaaacgatt
    10201 cggtctgaac gccttcaaaa tgcttggcgg tgcgtacgcc atcgctcaat tattgtgtga
    10261 aaaatatcat cttgatatcg aaacgctgtc atttgagcac ctgaaaaatg ccatcggcga
    10321 aaaaatgact ttcgcgacca ccaccgacgg caaccacggg cgcggtgtgg cgtgggcagc
    10381 acagcaactc ggacagaatg cggtgattta catgccgaaa ggttctgctc aggaacgcgt
    10441 tgacgccatt ctgaacctcg gtgccgagtg catcgtcacg gatatgaact atgacgatac
    10501 cgttcgcctg accatgcaac acgcgcagca gcacggctgg gaagtggtac aggacacggc
    10561 atgggaaggt tacaccaaaa tcccaacctg gatcatgcaa ggctacgcaa ccctggcaga
    10621 tgaagccgtc gagcaaatgc gtgaaatggg cgtaaccccg acgcacgttc tgctgcaagc
    10681 cggtgtcgga gcaatggccg gtggtgtgct gggttatctg gtcgacgtct acagcccgca
    10741 aaatctgcac agcattattg ttgaacctga caaagctgac tgtatttatc gctccggcgt
    10801 caaaggcgac atcgtcaacg ttggcggtga tatggccacc atcatggcgg gcctggcctg
    10861 tggcgaacct aacccgctgg gctgggaaat cctacgtaac tgcgccaccc aattcatctc
    10921 ctgccaggac agcgttgccg cattaggtat gcgcgtgctg ggtaatccgt acggcaacga
    10981 cccgcgcatc atctccggtg aatccggcgc tgtcggtttg ggcgttctcg cagcggttca
    11041 ttatcacccg caacatcaaa gcctgatgga aaaactggcg ctggacaaag atgccgtagt
    11101 gctggttatc agcactgaag gcgacaccga cgtgaagcac taccgcgaag ttgtctggga
    11161 aggcaaacac gctgtagcac cttaatcacc ctattggaac tactccctgc aaaacgggga
    11221 gtaaaaaatc tggagaaaaa taatggctaa gaatattcca ttcaaactga ttcttgaaaa
    11281 agcaaaagat taccaggcgg acatgactcg cttcctgcgc gacatggttg ctattcccag
    11341 tgaaagctgc gacgaaaaac gcgtagtaca tcgtattaaa gaagagatgg aaaaagtcgg
    11401 cttcgataaa gttgaaatcg acccgatggg caacgttctc ggttatatcg gccacggccc
    11461 gcgtctggtg gcaatggacg ctcatatcga taccgtcggc attggcaaca tcaaaaactg
    11521 ggacttcgat ccgtacgaag gcatggaaac tgatgagcta atcggtggtc gcggtacttc
    11581 cgaccaggaa ggcggcatgg catctatggt ttatgccggt aaaatcatta aagacctcgg
    11641 tctggaagat gaatataccc tgctggttac cggtactgtg caggaagaag actgcgacgg
    11701 tctgtgctgg cagtacatta ttgaacaatc cggcattcgc ccggaatttg tggtcagtac
    11761 cgaaccaacc gactgccaga tataccgtgg tcagcgcggt cgtatggaaa ttcgtattga
    11821 tgttcagggt gttagctgcc acggttctgc gccagaacgc ggtgacaacg ccattttcaa
    11881 aatgggtccg attcttggcg aattacaaga actctcccaa cgtctgggtt atgacgaatt
    11941 cctcggcaaa ggcaccctca ccgtttctga aatcttcttc acatccccaa gccgttgcgc
    12001 tgtagcagat agctgcgccg tctctattga ccgccgtctg acctggggcg aaacctggga
    12061 aggcgcgctg gacgaaatcc gcgccctgcc tgcagtacag aaagctaacg cggttgtttc
    12121 tatgtacaac tacgaccgtc cgtcctggac tggcctggtt tacccaaccg aatgctactt
    12181 cccgacctgg aaagtggaag aagatcactt caccgttaaa gcactggtga atgcctacga
    12241 agggctgttt ggcaaagcgc cggttgttga taagtggacc ttctcaacta acggcgtatc
    12301 tatcatgggc cgtcacggca ttccggtgat cggctttggc ccgggtaaag aacctgaagc
    12361 gcatgcacct aacgaaaaaa cctggaaatc tcacctggtg acctgtgccg cgatgtacgc
    12421 tgcaatcccg ttaagctggc tggcaacaga gtaattactc ttcacttatt cccctccggt
    12481 tcgccggagg gtttttggag tttgctatgc gcgtattgat caaaaacggc actgtcgtta
    12541 acgcagatgg acaagccaaa caggatttgc tgattgaaag cgggattgtt cgccagttgg
    12601 gcaacaatat ttcgccgcag ctcccgtatg aagaaattga tgccactggc tgttacgttt
    12661 tccctggcgg cgtggatgtc catacgcatt tcaatattga tgtcggcatc gcgcgcagtt
    12721 gtgatgattt tttaccggta cccgcgcagc tgcgtgtggc ggtacaacaa ccattattga
    12781 ccatatggga tttggcccaa acggctgtcg gttacgccat caactggagg tttatcgtgg
    12841 ttatgccgcc cataaagcgg tcatcgatta cagctttcac ggtgtgatcc agcacattaa
    12901 tcacgcaatc ctcgacgaaa tcccgatgat ggtcgaggaa ggactgagca gttttaaact
    12961 ctatttaacc tatcaataca aactcaacga tgacgaggtt ttgcaggcat tacgccgctt
    13021 gcatgaatcc ggcgcgctga ccaccgtgca cccggaaaat gatgcggcta tcgccagcaa
    13081 gcgggcggaa tttatcgccg cagggttaac cgcgccgcgc tatcatgcct tgagtcgccc
    13141 tctggaatgc gaagcggaag ccatcgcccg catgattaac ctggcacaaa ttgccggtaa
    13201 cgccccgctc tatatcgtgc acctgtctaa cggcttaggt ctggattatc tgcgtcttgc
    13261 ccgtgcgaat caccagccag tctgggttga aacctgccca caatatctcc tgttggacga
    13321 acgcagttac gatacagaag atggcatgaa gttcattctt agcccaccgc tgcgtaacgt
    13381 acgcgagcag gacaaactgt ggtgtggcat cagcgatggt gcgattgacg tggtggcgac
    13441 cgatcactgc accttctcga tggctcaacg cctgcaaatt tctaaaggcg atttcagtcg
    13501 ctgcccaaat ggcttacccg gtgtggaaaa ccgcatgcag ttactgtttt ccagtggcgt
    13561 gatgacggga cgtataacac cggaacgctt tgttgaatta accagcgcaa tgcccgccag
    13621 gctgtttggc ctgtggccgc aaaaaggatt attagcgccc ggttccgatg gcgacgtggt
    13681 gattatcgac ccacgtcaga gccaacaaat tcagcatcgc catctccacg acaacgccga
    13741 ctactcgcca tgggagggtt ttacctgtca gggcgcgatt gtcagaacct tatcccgtgg
    13801 tgaaacgatt ttctgtgacg gcacctttac aggcaaagcc gggcgaggtc gtttcctgcg
    13861 acgcaaaccg tttgtccctc ccgtgctcta accactgtca gggatctata cacaatcaag
    13921 aatgatgagt gaggaaaaat gagtaagaaa attgttctcg ccctgggcgg gaatgcgctg
    13981 ggcgatgacc tggccggaca aatgaaagcg gtaaaaatta cttctcaggc aattgttgat
    14041 ttaattgccc aaggacatga agttatcgtc actcatggta acgggccgca ggtgggcatg
    14101 attaaccaag cctttgaagc tgccgcaaaa actgaagcac actcgccaat gctgccgatg
    14161 tctgtctgtg ttgccttaag ccagggttat attggttacg atctacaaaa cgcgttaaga
    14221 gaagaactgc tttctcgggg cattaataaa cctgtagcaa cgctggttac tcaggtcgaa
    14281 gtcgacgcta acgatccggc attcctcaac ccgaccaagc cgatcggttc gttctttacc
    14341 gagcaagaag cagagcaact gacaaaacag ggttacactc tgaaagaaga tgctggtcgc
    14401 ggctatcgcc gtgttgttgc ctcgccaaaa ccggttgata tcattgaaaa agaaacggtt
    14461 aaagctctgg tagatgcagg ccaggtggtg atcgccgttg gcggtggtgg tatccctgtt
    14521 attcgtgaag gtaaccatct gcgtggtgcc agcgcggtta tcgataaaga ctgggccagc
    14581 gcccgtttag cagaaatgat tgatgccgat atgctgatca ttctgactgc ggtagaaaaa
    14641 gtggccatta acttcggtaa agagaatgaa cagtggctcg atcgcctgtc attaagtgat
    14701 gctgaacgct ttatcgaaga aggtcatttt gcgaaaggtt ctatgctgcc taaagtggaa
    14761 gctgccgcct cgtttgctcg ctctcgcgca ggtcgtgaag cactgattac cgtattgagc
    14821 aaagcgaaag aagggattga aggcaaaacc ggaacggtga tttgtcagta attttaaggc
    14881 cgggtgggta ataccatccg gccattttca tttaagccac ttccagcact tcttttgtcg
    14941 ctttcacgcc ctgatgcatc aacatcatta acgcctcaag tacgccgccg ccaatcgccc
    15001 gggctttatc agaaacgctg gtgaaatcag cggtttcacc acgaggatcg atatcaccaa
    15061 ttttaaaacc accaaccact gccaggccgt cgttcaacaa gccacgcacc atccccgtca
    15121 acggtgcttt aatttcatgc tcaccaatcc aggcaatcac atcgccctct ttcaccagat
    15181 cgcctaattt cacgttggat cgcataatgc ctgcagcagg agcacggatc acccgtcggg
    15241 tggtatgccc cataatattg ccaggaacat cggtattctc ctgcgcacaa ccagagtaaa
    15301 tcacctgacc gagccagtgc ccgcgatttg tttcaattac cgcatgacaa tccttccctg
    15361 cagtaaagcc cggcccaagc gcgattgtta ctggtgccat atctgcccgc gttcccaaat
    15421 tctgtttcgc cagaatagcg tccaccacgc aaagcggttt cagttcatca agcaatgaac
    15481 aggcgggatc taccatcaca gggatgaatc cgcgttcggt aagtttcatc gcttccgcag
    15541 agctggttgc caggcgagca gtgacgcctt cgaccgtcat ttcgccatcg aacacggcct
    15601 gggcaaacgc cacggtacaa cgaatcactg tcggtttttc cacttccagc atgatcactt
    15661 taaaacccgc atgatacaga cgtagcgcca caccactggc gatatctccc gcaccgcgaa
    15721 tcaccaccag ctggtgtccg gaaggatgag aaggtttcat catcagccca cccggcgcgt
    15781 tatttttcac ccataatatt tctgccagca cgctgatggc gatctcctgc ggcgtttccg
    15841 cacctatgtt gtaaccaacg ggcgcatgta aacgggcaat atgttcttca gccacgcctt
    15901 tctcacgcaa ttgacgcagg aaaagctgaa ccttgcggcg acttgccagc aaccccaacc
    15961 atgcaatggg ctgttcaatg agtttgtcga gggcttcacg atcctgatta ttcgtggcaa
    16021 tcaggacaaa attatcaggg cgaatatcca gttcttccac tgccgcacca aacgactcag
    16081 catggagaag cgtggttgat ggtgggaata gttcaggatt gaggctttcg cgataaatat
    16141 cggcaacggc aatatcaaat cctaatagcg ccgcactctg ggctatcgcc cggttgacat
    16201 gccccgcgcc aatcaacacc agacgtggac gcataccatg gacgctgata aatactgaca
    16261 ttgcacctcc acaatctgac ccgacagcat ccgcaccgtt acgagccata cgtccatgga
    16321 ataatcgcgg cttacgttcc tgcaatgcct gaagtgactc ttcaatcacc ttccgctcaa
    16381 ccattccgcc accaattgta ccgacgatag aaccatcggc gcgcactaac atttgtgcag
    16441 aatggcgggg agtcgagcct cggctatcaa caatttgcgc catcgcaaac ggacaatttt
    16501 gctcttcgag ttttgcagcc tctgtgaaaa tattcatacc aacctcaatg gatatccttt
    16561 cagtaacccg gaatacccgg gccgctaatt cacaaatctg cgcgcgattg caggatgttc
    16621 ttgtatatcg cccagccaga ttgcctctac gtcgtgttgt tgcagcggtt gaaggagctc
    16681 gctttgtgca atcgcattct cacattgaga aaaacggtta atgaaccaga ctcgccgaca
    16741 accttgcggt acgtttttaa acgccccctg agggtggcga accagcgcaa cgagatcgct
    16801 cagttgcaag gttgcatcag gtgttaaccc ggtaatatca gcaaactgcg accagcgatg
    16861 gacattttct gtgctcactt tcgcgcccaa agtatgtcct cccatcacag caatcacgca
    16921 gcaactgctt ttgggtatgc aaggttcgtg ctcatcaggc gcttttaacg gcattccacg
    16981 cgagccatct gcttcaatga gaattacgtc acactctggt cgttgcacca gtgcatcaat
    17041 cgcttctggc gtaaatccct gcacttttcc ctggttcgct ttccagctgt gaaaacaaaa
    17101 tgaaatgggg gatgtaagag acgcatgagg aagcatggcg ggatcacgac agaaaaccac
    17161 gggccagtga gacgttggca taaacatatg tgtcgtcgtc gtgattaaca cacgcctgcc
    17221 gctagcctgg agcagttctg ccagccaaaa aagcaagctg gttttccccc cagcaccaac
    17281 aacagaaatc acagtgggac gtttctgcgc acctaaatca atgactaatg ccgatgggtc
    17341 aactatactt ttcacgagtc tttatgacct ctctgggata aattatcccc aacttaatcc
    17401 atcaggaagt aacgcaatta tcaggcgtta ttagccccta taaataatgg aaccactatg
    17461 tcagccatcg actgtataat taccgctgcc ggattatcat caagaatggg gcaatggaaa
    17521 atgatgttcc cctgggaaca gggaacaatt cttgatacaa gtatcaaaaa tgcgttgcag
    17581 ttttgtagcc gaattatttt agtcaccggc tatcgtggta atgaactgca cgaacgttat
    17641 gcgaaccaga gcaatatcac tattattcac aacccagatt atgcgcaggg tttactaaca
    17701 tccgtcaagg ccgcagtacc cgcagtgcaa acagaacatt gttttctcac ccacggtgac
    17761 atgccaaccc tcaccatcga tatttttaga aaaatctggt cgttacgaaa tgatggcgca
    17821 atactgccac tccataatgg catccccggc catccgattt tagtgtcaaa accatgcctg
    17881 atgcaggcaa tccagcgacc caatgtcacc aatatgcgtc aggcactcct tatgggagat
    17941 cattattccg tcgaaataga aaatgcagaa ataattttag atattgatac tccggacgac
    18001 tttattacag cgcaaaaaag gtatactaaa atttagaaaa tgtagttaaa cgattgcgtt
    18061 caaatattta atctctccag cactctttat cataatgata aggaatcact gattttgaga
    18121 aaagagaaaa agtgataaat gggctgacaa taataatttt caggagtgat tttaattctc
    18181 atttggcacc tgtgagttca ttcacaaaaa gcctttaagt ttcgagccac ctcacatttt
    18241 tttatatttc ccgccaaacc tggcaagagt ggtgcgattg ttgctctatc cccctaaacc
    18301 accggatttc tcaacaccgg tcactcaatg atatctgtat aagctaagga gagggttatg
    18361 ggggatatta tgcgtcccat tccgtttgag gaacttttga cgcgcatatt tgatgaatac
    18421 caacaacaac gctcaatctt tggtattccc gagcaacagt tttactcacc tgtaaaaggt
    18481 aaaactgtta gcgtcttcgg tgaaacctgt gccactcccg tcggccctgc cgctggcccg
    18541 cacacgcagc tcgcgcaaaa tattgtcact tcctggctga ctggcggacg cttcatcgaa
    18601 ctaaaaaccg tccaaattct tgaccgcctg gagctggaaa agccctgtat tgatgccgaa
    18661 gacgaatgct ttaacaccga atggtctacc gaatttacct tgcttaaagc ctgggatgaa
    18721 tacctcaaag cctggtttgc cctgcacctt ctcgaagcga tgttacagcc ttctgattcc
    18781 ggtaaatcgt tcatctttaa tatgagcatc ggttacaacc tcgaaggtat taagcaaccg
    18841 ccgatgcaac agttcatcga caatatgatg gacgcatctg accatccgaa attcgctcaa
    18901 tatcgcgata cgctgaataa attactccag gatgacgcat ttttagcccg ccacggattg
    18961 caggaaaaac gcgaaaactt gcaagcctta cccgctcgca tccccaccag tatggtgcag
    19021 ggcgtccccc tctccaccat gcacggctgt cctccgcatg aaatcgaagc catttgccgc
    19081 tacatgctgg aagaaaaagg gctcaacacc tttgtcaaac ttaacccgac cttactgggg
    19141 tacgcgcgtg ttcgtgagat cctcgatgtc tgcggtttcg gttacatagg cttaaaagaa
    19201 gagtcatttg atcacgacct caagctgacg caagcactgg aaatgctgga gcgcctgatg
    19261 gtactggcaa aagaaaaatc actcggcttt ggcgtaaaac tgactaacac tctcggcacc
    19321 atcaacaata aaggcgcact gcctggtgaa gagatgtata tgtcaggccg tgcgctgttc
    19381 ccgctctcca tcaatgttgc agcagttctc tctcgcgcct ttgacggcaa actgcccatt
    19441 tcttattccg gtggtgccag tcagctgact atccgcgata tttttgatac aggtattcgc
    19501 cctattacta tggcaaccga cctgctgaaa cctggcggct atctgcgctt aagtgcctgc
    19561 atgcgcgagc tggaaggctc cgacgcctgg ggacttgacc atgttgacgt cgaacgactg
    19621 aacagactgg cagcagacgc gttaaccatg gaatacaccc agaaacactg gaagccagaa
    19681 gagcgtattg aagtggcaga agacctgccg ctgaccgatt gctacgttgc cccctgtgtt
    19741 actgcctgcg ctatcaagca agatattccg gaatacatcc gtctgcttgg cgaacaccgc
    19801 tatgccgacg cgctggaact catctaccaa cgcaacgccc tacctgccat taccggtcat
    19861 atttgcgatc accagtgcca atacaactgt acccgcctgg attacgacag tgcgctgaat
    19921 atccgcgaac tgaaaaaagt cgcgctggaa aaaggttggg atgaatataa gcaacgctgg
    19981 cacaaacccg ccggttctgg ttcacgccat ccggttgccg tgattggtgc aggtccggcg
    20041 ggtctggcag caggttactt ccttgccaga gcgggccatc cggttacgct gtttgaacgc
    20101 gaagccaatg ctggcggcgt ggtgaaaaat atcattcctc agtt
//
LOCUS       DAJTXQ010000077        19996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000077 DAJTXQ010000000
VERSION     DAJTXQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.077"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..487
                     /gene="radC"
                     /locus_tag="OO515_002591"
     CDS             <1..487
                     /gene="radC"
                     /locus_tag="OO515_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6576489.1"
                     /translation="EYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLLSPE
                     MTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAIKIN
                     ASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGW
                     I"
     gene            704..940
                     /gene="rpmB"
                     /locus_tag="OO515_002592"
     CDS             704..940
                     /gene="rpmB"
                     /locus_tag="OO515_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6576490.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            961..1128
                     /gene="rpmG"
                     /locus_tag="OO515_002593"
     CDS             961..1128
                     /gene="rpmG"
                     /locus_tag="OO515_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6576491.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1226..2035
                     /gene="mutM"
                     /locus_tag="OO515_002594"
     CDS             1226..2035
                     /gene="mutM"
                     /locus_tag="OO515_002594"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6576492.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2074..2553)
                     /gene="coaD"
                     /locus_tag="OO515_002595"
     CDS             complement(2074..2553)
                     /gene="coaD"
                     /locus_tag="OO515_002595"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6576493.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2561..3838)
                     /gene="waaA"
                     /locus_tag="OO515_002596"
     CDS             complement(2561..3838)
                     /gene="waaA"
                     /locus_tag="OO515_002596"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6576494.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4251..5309
                     /gene="rfaQ"
                     /locus_tag="OO515_002597"
     CDS             4251..5309
                     /gene="rfaQ"
                     /locus_tag="OO515_002597"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6576495.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5306..6430
                     /locus_tag="OO515_002598"
     CDS             5306..6430
                     /locus_tag="OO515_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6576496.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6423..7220
                     /gene="rfaP"
                     /locus_tag="OO515_002599"
     CDS             6423..7220
                     /gene="rfaP"
                     /locus_tag="OO515_002599"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6576497.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7236..8252
                     /gene="waaO"
                     /locus_tag="OO515_002600"
                     /gene_synonym="rfaI"
     CDS             7236..8252
                     /gene="waaO"
                     /locus_tag="OO515_002600"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6576498.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8269..9264
                     /locus_tag="OO515_002601"
     CDS             8269..9264
                     /locus_tag="OO515_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6576499.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9274..9966
                     /gene="rfaY"
                     /locus_tag="OO515_002602"
     CDS             9274..9966
                     /gene="rfaY"
                     /locus_tag="OO515_002602"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6576500.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            9992..11017
                     /locus_tag="OO515_002603"
     CDS             9992..11017
                     /locus_tag="OO515_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6576501.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11102..12085
                     /locus_tag="OO515_002604"
     CDS             11102..12085
                     /locus_tag="OO515_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6576502.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12131..13384
                     /locus_tag="OO515_002605"
     CDS             12131..13384
                     /locus_tag="OO515_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6576503.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13454..14425)
                     /gene="rfaC"
                     /locus_tag="OO515_002606"
     CDS             complement(13454..14425)
                     /gene="rfaC"
                     /locus_tag="OO515_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6576504.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14429..15475)
                     /gene="rfaF"
                     /locus_tag="OO515_002607"
     CDS             complement(14429..15475)
                     /gene="rfaF"
                     /locus_tag="OO515_002607"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6576505.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15485..16417)
                     /gene="rfaD"
                     /locus_tag="OO515_002608"
     CDS             complement(15485..16417)
                     /gene="rfaD"
                     /locus_tag="OO515_002608"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6576506.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16631..17827
                     /gene="kbl"
                     /locus_tag="OO515_002609"
     CDS             16631..17827
                     /gene="kbl"
                     /locus_tag="OO515_002609"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6576507.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17837..18862
                     /gene="tdh"
                     /locus_tag="OO515_002610"
     CDS             17837..18862
                     /gene="tdh"
                     /locus_tag="OO515_002610"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6576508.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTVICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19101..>19996
                     /gene="waaH"
                     /locus_tag="OO515_002611"
     CDS             19101..>19996
                     /gene="waaH"
                     /locus_tag="OO515_002611"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6576509.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5396 a         4358 c         4724 g         5518 t
ORIGIN      
        1 tgaatatgaa caatttagtg gcgttcatgg aattggcgta gcgaaatttg cccagttaaa
       61 ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag aaagcccttt
      121 actcagcccg gagatgacgc gggaattttt acaaagccag ctcacgggtg aggagcggga
      181 gatctttatg gtgatctttc tcgactcaca acaccgggtt ataacgcata gccgtctttt
      241 ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccggg aagcgataaa
      301 aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg ctgaacccag
      361 taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca tggatttacg
      421 cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg
      481 gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact tgagcacatc
      541 gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt tgggcctggc
      601 aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga ggcgccaata
      661 ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc gagtctgcca
      721 agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga acgcgactaa
      781 acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg aaaagcgttt
      841 tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac
      901 agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag gaaataaatc
      961 atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac tggtcacttc
     1021 tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa aaaattcgat
     1081 ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga
     1141 tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc catattgatt
     1201 gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg
     1261 cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc gcaacggacg
     1321 cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag tgcttagcgt
     1381 gcagcggcgg gctaaatatc tgctgctgga gctgcctgag ggctggatta tcattcattt
     1441 ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa agcatgacca
     1501 tgtggatttg gtgatgagca acggcaaagt gctgcgctac accgatccgc gccgctttgg
     1561 tgcctggctg tggaccaaag agctggaagg gcacaacgtg ctggctcatc ttggaccgga
     1621 gccgcttagc gacgatttca atggtgagta tctgcatcag aagtgcgcga agaaaaaaac
     1681 ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga atatctatgc
     1741 cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat cactgtcgct
     1801 ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca
     1861 gggtggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct atttcgcgca
     1921 ggaattgcag gtttacggac gaaaaggtga gccgtgtcgg gtgtgcggta cgccgattgt
     1981 ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga agtaattcat
     2041 acgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca ccagcgcctg
     2101 atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca cctctttcac
     2161 caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac tttccagttc
     2221 cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat ccgccaccgc
     2281 acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc
     2341 actaaaccca accacttcca cgttccccag atgcgcggtt gcctgctgtg tcagcgccac
     2401 acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg ccagaataac
     2461 gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg taatgggatc
     2521 gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt ttcggtggca
     2581 ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga tacagtactt
     2641 caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa gaggaaacct
     2701 cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc tccagccgcg
     2761 cgcaaatgtc tttgaaatta aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg
     2821 cagcttccag aggattatgc cctccacgtt caaccagtga accgccaaca aaggcgagat
     2881 cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc
     2941 tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc tggcggacaa
     3001 ggttaatcgc atccgggaag cgttctggat gacggggtac caggatgagc aataaattcg
     3061 ggaattgctg taataatgcc tgatgtgcgg cgatcaccac actctcttca ccttcgtgag
     3121 tgctggtggc aatccatacc gggcggtgtg gtgcccactg gcggcgcagc gtcacggctt
     3181 tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta acggttacct
     3241 ggttattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt tgcgcagcaa
     3301 tcagcgtaat acgacgcagt aagcggcgga cgaatttacc cagtttggca taacctgcgg
     3361 ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttatgtagcg
     3421 ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagtttg gggtcgactt
     3481 tattcaggaa acggttgagc gcatcgggca ggtcatacgg cagataaacg tgctgaacat
     3541 ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa
     3601 tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc gccagagttt
     3661 caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac
     3721 cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc
     3781 gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag
     3841 taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttaccatt
     3901 acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg aacatctata
     3961 ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga aggaatcgca
     4021 gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc tttattgaaa
     4081 atcaacaatg tgcttaaaaa gcattcgggt cggattgccg cgaaagaagt gatacactag
     4141 cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg gcggtagcgt
     4201 gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt gtggataagc
     4261 catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta ttaactactc
     4321 ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc
     4381 aggacaccac cccgatcctg tctgaaaatc cagagattaa cgcgctctac ggcataaaaa
     4441 ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc aaggtattac
     4501 gtgccaataa gtatgacctt atcgtcaatc ttaccgatca atggatggtt gctatactgg
     4561 ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg cagtctgctt
     4621 tttggcgtaa aagtttcacc catttagtgc cgttgcaggg tggaaatgtg gtggaaagta
     4681 acttatccgt gctgacacca ttgggacttg attcgttggt gaagcagaca accatgagtt
     4741 acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa
     4801 attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac aacgccaagt
     4861 tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg acgtccggcc
     4921 cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag acgccaccag
     4981 taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc
     5041 agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc
     5101 tgatatcgct gtttggtgcg acagaccata ttttctggcg tccttggtca aataacatga
     5161 ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg
     5221 agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat aaattactgc
     5281 cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata aatattttcc
     5341 ctttggcggt ttgcagcgcg attttatgcg tattgctcag acagtcgccg cccgaggtca
     5401 tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat ttgaactgat
     5461 caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt ttgcctgggt
     5521 gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca aaatgccggg
     5581 gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtcgcgc aggaaaaagg
     5641 ctttttctat cacctgacgt cacgttatcg ccattatgcc gcctttgagc gggcaacctt
     5701 cgaacagggc aagccgacac agctgctgat gctgacagat aagcaaatcg ccgatttcca
     5761 gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga tttatcctga
     5821 tcgtaaatat agccagcagc cagctaatag ccgtgaaatc ttccgtaaga agaatggaat
     5881 aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga
     5941 tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca cattgctata
     6001 tgttgttgga caggataagc cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg
     6061 cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg gaattaatgg cggcggcgga
     6121 tttattactg catcctgcct accaggaagc agcgggaatt gtgctactgg aagcgattac
     6181 tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata ttgtcgacgc
     6241 caattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg agattttacg
     6301 caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc
     6361 tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa cgggtggtct
     6421 ggatggttga acttaaagag ccgtttgcca cgttatggcg cggtaaagat ccttttgagg
     6481 aagttaaaac cttgcagggt gaggtttttc gtgaactgga aactcgccgc actctgcgat
     6541 ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc ctgaaagaga
     6601 taatcaaaaa tttactctca ttgcggatgc cagtattagg cgcagaccgc gaatggaatg
     6661 cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca tttggcgaaa
     6721 aaggtattaa tccgctgagc agaacctcgt ttattattac cgaagatctg accccaacca
     6781 tcagtctgga agattactgt gctgactggg cgaccaaccc accagatgtt cgcgtaaagc
     6841 gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgtg ggcattaacc
     6901 accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt aaggaagagg
     6961 agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc
     7021 gttggcgcga taaagatctt attgggcttt atttttcttc gatgaatatc ggcctgactc
     7081 agcgggatat ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa gacattctca
     7141 agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa
     7201 cgattcgaaa atcgttgtaa tttccggaga gaaatatgag tgcccactat tttaatccac
     7261 aagagatgat caataagaca atcatcttcg atgaaaggcc agcggcgtca gtggcatcat
     7321 cattccatgt tgcttatggc attgataaaa actttctttt tggttgtggt gtttcaatca
     7381 cgtcagtttt gttacataac aacgacgtga gttttgtttt ccacgttttt attgatgata
     7441 tccctgaagc cgatatccag cgtttagccc aattggcgaa aagctatcgt acctgtatcc
     7501 agatccatct ggtaaattgt gaacggctta aggcattacc gacgaccaaa aattggtcta
     7561 ttgccatgta tttccgtttt gtaattgcag attactttat tgatcaacaa gataagatcc
     7621 tttacctgga tgctgatatc gcctgtcagg gaaacttaaa gccgctgata acaatggatc
     7681 ttgccaataa cgttgctgct gttgttactg aacgcgatgc taactggtgg tcgttacggg
     7741 gtcaaagtct gcagtgtaat gaacttgaaa agggttactt taattcaggt gtcctgttaa
     7801 ttaatacact agcgtgggcg caggagtccg tttctgctaa agcgatgtcg atgcttgctg
     7861 ataaagccgt cgtttcccgt ttaacctata tggatcaaga tatacttaat cttatcctgt
     7921 cagggaaagt taaattcatt gatgctaaat acaatactca atttagttta aattatgaat
     7981 taaaaaaatc atttgtttgt ccaattaatg atgaaaccgt attaattcat tatgtcggcc
     8041 cgacaaaacc ctggcattac tgggccggtt atccaagtgc gcaacctttt atcaacgcca
     8101 aagaagcatc gccctggaaa aatgaaccgt ttatgcggcc aattaattca aactatgctc
     8161 gttattgcgc caagcataat tttaaacaaa atgaaccaat taacgggata atgaattata
     8221 tttattattt ttatttaaag ataataaaat gatattgaga aaagttaaat gaatgaattt
     8281 ataaaagaac ggttttcgta tttagcagat aataaaaaag aaaacgcccc agaactaaat
     8341 gtttcctacg gtatcgataa gaattttttg tatggtgctg gcgtttcaat ttcttccgtt
     8401 ttgattaata attcagatat taattttgtc tttcatgttt tcactgatta tgtggatgat
     8461 gattatttaa agtcatttaa tgaaacagca aaacaattta atacctcaat tattgtatat
     8521 ttaattgacc caaaatactt tgctgatctg ccgacgtcac agttttggtc gtacgcgaca
     8581 tacttcaggg tattgtcctt tgaatatctg agtgaaagta tttccacact gctgtatctg
     8641 gatgccgatg tcgtttgtaa aggaagtctg aagcctctca cagaaattat atttaaagat
     8701 gagtttgctg cggtcattcc tgacaatgtt agtactcagg cggcatgtgc aaaacgcctc
     8761 aacattcccg agatgaatgg acgttatttc aatgcaggcg ttatctatgt caatattaaa
     8821 aaatggcatg aagcaaattt gacaccgtat ttactcacgc ttttacgagg ggaaactaaa
     8881 tatggctctc ttaaatattt agatcaggat gcgttgaata tcgcatttaa tatgaataat
     8941 atctacctcg cgaaggattt tgatactatt tataccctga aaaacgaact tcatgatcgt
     9001 agtcatcgaa agtatcagca aaccattacc gataaaacag tattgattca ctatacaggg
     9061 ataactaaac catggcatag ctgggctggt tatccgtctg catcatactt taatatcgcg
     9121 cgtgaacaat ctccctggaa gaaatatcct cttaaagagg cgcggactgt tgcagaaatg
     9181 cagaaacaat ataagcatct gtttgcccat ggtgagtata ttaaaggcat aacttcatta
     9241 attaagtaca agcttaagaa ataaattact cttatgatta caagtatacg ctatcgcggc
     9301 ttctcatttt attacaaaga taatgataat aaatataaag aaatctttga tgaaatactg
     9361 gcctataatt ttaaaacagt aaaagtatta cgtaatatta atgataccaa agtatcattg
     9421 attgatacga aatattgtcg ctatgttttt aaagtctttg ccccgaaaac gaaaaggaat
     9481 gaacgttttt taaaatcttt tgtaaaaggt gactactatc agaatttgat cgttgaaacc
     9541 gatcgtgtca gaagtgcagg tcttactttt cctaatgatt tttattttct ggctgagcgt
     9601 aaaattttca actatgccag tgtttttatc atgcttatcg agtatgtcga gggcgtagag
     9661 ctcaatgata tgccgatcat tccagaagat gttaaagcag aaattaaggc ttcaatggaa
     9721 aaattgcatg ccttaaatat gctatcgggc gacccacacc ggggtaactt tatcgttagt
     9781 aaagatggtg tcaggataat tgacctatcc ggtaaaagct gtacggcaga gcgtaaagct
     9841 cgtgatcgtc tggccatgga acgtcacctg ggtattgcca acgaaataaa agattatggc
     9901 tactattcag taatctacag aaccaaacta cgcaaattta ttaaaaaatt aaaaggcaaa
     9961 gcgtaaacca cacagtcaaa acggaaccaa catggattta ttagctgaga gtattactga
    10021 agtcgctgtc tctggggaaa ttgctaacac cgatcgtgtg ttaaatatcg cttacggtat
    10081 tgaccgtaac tttttatttg gtgcggcagt atctatgcaa tcagttgtta tgcataatcc
    10141 ggaccttgcg gttaagtttc atctctttac cgactacatt gatgaagatt atctacaacg
    10201 tgttaatgct tttaccagca aaaatgctaa cgttgaagta agaatttata aagtctccag
    10261 tgcctttatt gatatcttcc ccagcctgaa acagtggtct tatgcaacat tcttccgttt
    10321 agttgcgttc cagtatctga gtgaaactat tgaaaatctg ttatatatcg atgctgatgt
    10381 catctgtaaa ggctcattag ctggattgct tgatattaat tttgatgggg ataagtttgc
    10441 agctgttatt aaagatgtgc cttttatgca ggaaaaacca gcgaagcgtc tggctataga
    10501 gggacttcca gggaattatt tcaacgccgg tgtagtatat ctgcagcttg aagcatgggc
    10561 gaaaaatgat tttatgaata aagccattgc tatgctggca agtgacccgc agcacacgaa
    10621 gtataaatgc cttgatcagg atattttaaa tattctgttc tttggtcatt gtatttttat
    10681 tagcggcgat tatgattgct tttatggcat cgattatgag ttaaaaaata aaagcgatga
    10741 agattataaa aagaccatta ccgatgatac taagctgatt cattatgttg gcgtaacgaa
    10801 gccctggaac gactggacga attatccctg ccagaagtat tttaatgagg cttatcaggc
    10861 ttcttgctgg aatgatgtgg cgtttattcc agccacgaat gaaaagcagt atcaagtgaa
    10921 atatcaacat gcaaagaaaa atggtgatac gtttaacgct tttatttact ttattaaatt
    10981 taaattaaat aagtataaaa gaaaactatt tggataataa taaagatcat ggatgtctga
    11041 gttacaataa taattaagga tgagtactat catattttat tagttcaggc aagagaacac
    11101 gatgagcaat gattaccctt tagtatccat aataataccg acgtataatt catctgatta
    11161 cattactgaa actctaacga aattagaaaa acaaacttac ccaaattttg aaattgttat
    11221 tgttaatgat ggttctaaag ataacacatc aaacgttttg agagagtatg ggttaaccca
    11281 ctctcgatta attattatca ataaagaaaa tggcggtgtt tcgtctgcca ggaatacagg
    11341 tatccgcaag gcgcaaggac agtttatatg ttttatggat gatgatgatg agatagatcc
    11401 taactatctg ctgaagatgt ataccagaca acatgagacg ggaggggatg cgatttattg
    11461 tgggctttat ggccattata taaaagatgg tgttacttac tcacctataa atacagagtt
    11521 taatgaagga tctttacttt tcgacttttt ttataaaaag gttagattcc atatagggtg
    11581 cttgtttata agaaaacaac ttctggaaga gaataatctt ttttttgatg aagatttacg
    11641 actaggagaa gatctggatt ttatctatcg actgctaatt acatgcgata tgtatgcggt
    11701 tccatattat atgtataagc ataactatag agaaaattcc ttaatgaact catgtagaac
    11761 catcactcat tatcgacatg agtcatttgc gcacgaaaga atctactctt ctgtgatgca
    11821 gttatacaaa ggtaacagga aagaagaaat tcatacatta ttgagtcaaa atagagctta
    11881 tcataaaact cgttatttat ggaatgttct acttaatggt gattttgaac tattaaatca
    11941 attagttgaa agcaatgaaa aagaattgaa tgattgtaat ctttctggca agagagataa
    12001 aagacgagca aaaatattag catcaaaaaa ttatattctc tggaggatgg taagactggt
    12061 aaataggaaa aagaataaac gttagatctt gcattaaata atccgtcctc gtagcatagg
    12121 ttgaaactat atgtcatttt gttggaatga aattaattct ggtataaagt ctttaattct
    12181 catattatgt attttttctt tgatgacttt gtctttatgg gatgatgttg caacaaagtt
    12241 tcttcatgca gctggaatta tatctgcatt gtattttcta ataacaccaa aaaaaacaat
    12301 aactaacaat cctactttgt taattttcat ctcattatgt cttttgggaa tcataaatat
    12361 catctggtat tcacattata aagtttcagg ctctgtttat accaatgcat atcgtggccc
    12421 aatggaaact gggaaaattg ccttgtgtag tgcttttatt ttcttggttc tttttgctaa
    12481 aaatgagttg agaacaaaaa taaaatttgg gaaactaatt ctgttcgcat ccctggcaac
    12541 gcagttactt ttttttgcgc atgccatgtg gcaacatttc tatttaaacg ttgaccgtgt
    12601 tgcattatca gcttcccacg ctacaacagc aggctacatc atccttttcc cttctttact
    12661 ggcatcaatt ctcattttaa aatccgactt tagacataaa acaacattat atacaattaa
    12721 cttcatgctt agcttatgtg ctgtcatagt aactgaaacg cgtgcagcca tattagtgtt
    12781 tccattcttt gcgttactat taatcgtaat ggatagttat attaataagc gaattaatta
    12841 taagttatat tgttttattg cgattgcatt attagcaggt gtattttctt ttaaagatac
    12901 attgcttatg agaatgaata acttaaataa cgatttagtt aattattcgc atgataacac
    12961 cagaacttca gtcggtgccc gtctggcaat gtatgaagtt ggcttaaaaa catattctcc
    13021 aataggacaa tcactggaaa aacgagcgga aaaaatacat gaactagaag aaaaagagcc
    13081 tagattgagt ggcgctttac cctatataga ttctcatttg cataacgatc tcatagatac
    13141 gttatcaacg cgtggtattc ctggagttgt attaacaatt ttagcctttt cagcaatact
    13201 catatatgcc ttaagaactg ctaaagaacc ttatatttta atcttgcttt tttcactact
    13261 ggtagtaggc ctaagtgatg taatactctt ttctaaaccg gttccgactg ctgtgtttgt
    13321 caccataata ttgctttgtg cttattttaa agcacaatca gaccaatgtt tattagataa
    13381 ataatgaata tgtgaaataa aatcagcaag gaggctgatt ttatttcaaa attatctatt
    13441 aaggggttgt aatttaaatc atagcaacgt tttcttcaat gaatcgctta acagcatttg
    13501 ctgtcaattg agacaactca ttccccggag ccctacaaac catctgattc ttcccatatc
    13561 caccaattaa tcccggatcg gttggtccat aaaccgtgat attgggtcta tccagtgccg
    13621 ccgttaaatg gcttaacccc gtatccaccg acactacaaa tttagccccg gccagcacgc
    13681 gggcaacgcc ttccagactc atcttcggca atacttcaac ataagcaaat ccttccgcca
    13741 gtcgtttcgc ccgctcttcc tcatgcggcg cgccccacgg aagtttaatc cgtattcctg
    13801 aatcagccag taaaccaatc aattctcgcc agtgttcttc cggccagtgt ttatcatcac
    13861 gggtcgtcgc atgaagaaat acggcatatt cgccagcatc tgtaggcagg ttcgtcagga
    13921 aatgctgtgc aatagcataa tcgccctggg tttgcggttt gctatagccc agacttttgg
    13981 caaacagttc gcgggtgcgt tctacggcgt gctgctgttt tgcaatatga tgcttacgat
    14041 tgtaaaacag actggctaaa ggttcgcgag cggtttgcca gtccatgcca tgctttacgc
    14101 catgcgccag acgcgtcacc agcgccgcgc tttttaccag cccctgagcg tcgataactg
    14161 cgtcatagtt ctctgcttgt agcgcttcac gaaacgcttt gcgttccgct tttatggggg
    14221 ccgagaacca ggctttacgc cagcgacgta ttgccacagg aataactcgc tcaacggcgg
    14281 cgtgccagga aggaatctgt gcgaaccctt cttccaccac ccagtcaaac ttaatccctg
    14341 ggattgcctg ctgggcatca gtgagtgcgg gcaacgtatg gagaacatcg cccatcgacg
    14401 atgttttaac gatcaaaacc cgcatccgtc aggcttcctc ttgtaacaat agcgcgttga
    14461 gttcttccag tacgcgctgg ggagtaatgt cgattaagct ctggtgataa ccctccgcag
    14521 cgtcaccttt acgcactttg tgatagccgg taatcaggcg gatcacgcgc gctttatggg
    14581 atagcggcgg tgtgaagtcc gggctactcg gaccatacag ggcaaccagc ggacgattga
    14641 gcgccgccgc aacgtgcatc aggccagaat cgttagtgac aatggcttta caggctgcaa
    14701 tcaggataac cgcttgatca agctgtgttt cccccgccag gttccgacac catgcctgct
    14761 gctcggtatt caaagcggca agaatctcat tgcccgcttc atgatctttc gccgagccaa
    14821 acagaaccac ctgataacct tcatcaatca gctgctttgc cagttccgca tagtggtagt
    14881 gtggccagcg ttttgccgga ccaaactccg cacccgggca aaaaccaatc atcggacgtt
    14941 ctgatgaaag cgaaaattga ttacaggtat atgatttttc accttcgctc acctgcaatt
    15001 gcggccataa caacggtttt ggtagatctt gtgcggtacg catcacgcct ttgtcatagg
    15061 ccagcgcgac atagcgttcc accattagcg gccaggcttc tttatcgagc acgcgtaaat
    15121 cgttgagtaa accgtagcgc atctcgccgc gccagccggt gcgatgagga atacccgcga
    15181 agaaaggcac taatgcagat ttgaaggagt tgggtaagac gtaggcgcgg tcgtagcgct
    15241 tttcacgcag gctatgaccc agtttgcggc gttcgccgat ttccagcgct ccgtgaccga
    15301 gaggcatagg aatagcttcg ttaacttccg gcatccgcga taataatgga cggcaccatg
    15361 ccggtgccat cacatcgatt atcgcctggg gatagcgcgc ctggagcgtg cgatagagac
    15421 tttgcgacat catcatgtcg ccaacccaag acgggccgat caccagtatt ttcatgcaga
    15481 gctcttatgc gtcgcgattc agccaggcca tgtattccgt tacaccttca gcaacggttt
    15541 tgaacggttt gtcgtaaccc gccgcgcgca gatttgtcag atctgcctga gtgaacgcct
    15601 ggtagcggcc tttcagctta tccgggaacg gaatgtattc gatctggcct ttcttgtgat
    15661 aagccagcgt agcatcagct acagcctgga aggattccgc acgaccagta ccgaggttga
    15721 agatgccgga aacgccattt tccaggaacc acagattcac atcagccacg tcgcccacat
    15781 agacgaagtc gcgtttgaag ttctcgctac cttcaaacag cttcggtgat tcaccgttgt
    15841 taagctgggt gttgagatgg aaagcgacgc tcgccatgct gcctttatgg ccttcacgcg
    15901 gtccataaac gttgaaatag cggaagccaa caatctgcga gttagcttca ggcaggattt
    15961 ggcgaacata ttcatcaaac aggaattttg agtaaccgta gacgttcaac ggtttttcgt
    16021 actcgcggga ttcaataaag tcggaggtgc gtccgccgta ggtggctgcg gaagaggcat
    16081 acaggaacgg gatttcgcgc tccagacagt agtgcagcag ctctttggag tattgatagt
    16141 tgttatccat catatacttg ccatcccact cggtggtgga agagcacgcg ccttcgtgga
    16201 aaatcgcttc gacatcgccg aactcttcgc cagccataat ctggatcagg aagtcttcct
    16261 tatccatata gtccgcgata tccagatcca ccaggttcac aaacttggtg ccgtctttca
    16321 ggttgtccac caccagaata tcggtgatgc ctttatcatt cagggcctta acgatgttgc
    16381 tgccgataaa gcccgcgccg ccggtaacga tgatcataac tgtaaccttc gaattatgga
    16441 gtcagagaca atctcagaca cgaatacttc tatcatatca ctacacggcg ttggcttcag
    16501 ccattcgcca aaagtgagat tgatggacgt tatttatcct gaattttgca gaagtgttaa
    16561 cgcgtcatct cgtcgcgacc tataagtttg ggtaatatgt gctggaattt gccctgtctg
    16621 gagaatcgca atgcgtggag aattttatca gcagttaacc aacgatctgg aaaccgcacg
    16681 ggcggaaggg ttgtttaaag aagagcgcat tattacgtct gcgcagcaag cagatatcac
    16741 tgtggctgat ggaagccacg tcattaactt ttgtgccaat aactatctcg ggctggcgaa
    16801 ttatccggat ctgattgcgg cggcaaaggc gggaatggat tctcacggtt tcggcatggc
    16861 ttcggtgcgt tttatttgcg gcactcagga cagccataaa gagcttgaac aaaaactggc
    16921 ggcattcctg gggatggaag atgcgattct ctactcttcc tgctttgatg ctaacggtgg
    16981 cctgtttgaa acgctgctgg gcgcggaaga cgccattatc tccgacgcac tgaaccacgc
    17041 gtctattatt gatggtgtgc gtctgtgtaa agctaaacgc tatcgctatg ccaacaacga
    17101 tatgcaggag ctggaagcac gtctgaaaga agcgcgtgaa gccggtgcgc gtcatgtgct
    17161 gattgccacc gatggtgtgt tctcgatgga cggcgtgatt gccaacctga agggcgtttg
    17221 cgatctcgca gataaatatg atgccctggt gatggtagac gactcccacg cggtcggttt
    17281 tgtcggtgaa aatggtcgtg gttcccatga atactgcgat gtgatgggcc gggtcgatat
    17341 tatcaccggc acgctcggta aagcgctggg cggggcttct ggtggttata ccgcggcgcg
    17401 caaagaagtg gttgagtggc tgcgccagcg ttctcgtccg tacctgtttt ccaactcgct
    17461 ggcaccggcc attgttgccg catccatcaa agtactggag atggtcgaag cgggtagcga
    17521 gctgcgtgac cgtctgtggg cgaacgcgcg tcagttccgt gagcaaatgt cggcggcggg
    17581 ctttaccctg gcgggagccg atcacgccat tattccggtc atgcttggtg atgcggtagt
    17641 ggcgcagaaa tttgcccgtg agctgcaaaa agagggcatt tacgtcaccg gtttcttcta
    17701 tccggtcgtt ccgaaaggtc aggcgcgtat tcgtacccag atgtctgcgg cgcatacccc
    17761 tgagcaaatt acgcgtgcag tagaagcatt cacgcgtatt ggtaaacaac tgggcgttat
    17821 tgcctgagga tgtgagatga aagcgttatc caaactgaaa gcggaagagg gcatctggat
    17881 gaccgacgtt cctgtaccgg aactcgggca taacgatctg ctgattaaaa tccgtaaaac
    17941 agtcatctgc gggactgacg ttcacatcta taactgggat gagtggtcgc aaaaaaccat
    18001 cccggtgccg atggtcgtgg gccatgaata tgtcggtgaa gtggtaggta ttggtcagga
    18061 agtgaagggc ttcaagattg gcgatcgcgt ttctggcgaa ggccatatca cctgcggtca
    18121 ctgtcgcaac tgtcgtggcg gtcgtaccca tctgtgccgc aacacgatcg gcgtcggcgt
    18181 taaccgcccg ggctgcttcg ccgaatatct ggtgattccg gcgttcaacg ccttcaaaat
    18241 tcccgacaat atctccgacg acctggcttc catttttgat cccttcggta acgccgtgca
    18301 taccgcgctg tcgttcgatc tggttggcga ggatgtgctg gtctctggtg caggcccgat
    18361 tggtattatg gcagcggcgg tggcgaaaca cgttggtgca cgcaatgtgg tgatcactga
    18421 tgttaacgaa tatcgtctcg aactggcgcg caaaatgggt atcacccgtg cggtcaacgt
    18481 cgctaaagaa aaccttaatg atgtgatggc agaactgggc atggccgaag gctttgatat
    18541 cggtctggaa atgtccggtg cgccgccagc gtttcgtacc atgctcgaca ccatgaacca
    18601 cggcggtcgc attgcgatgc tgggtattcc accgtccgat atgtctatcg actggaccaa
    18661 agtaatcttt aaaggcttgt tcattaaagg tatttacggt cgtgagatgt ttgaaacctg
    18721 gtacaagatg gcggcgctga ttcagtcagg tctggatctc tcgccgatca ttacccatcg
    18781 tttctctatc gatgatttcc agaagggctt tgacgctatg cgttcgggcc agtccgggaa
    18841 agtaattctg agctgggatt aacacgaaca agggctggta ttccagccct tttgcctgag
    18901 gataatccgt taaatatgta aaatcctgtc agtgtaataa agatatcgta attgcgctga
    18961 tctcttatat agctgctctc attatctctc taccctgaag tgactctctc acctgtaaaa
    19021 ataatatctc acaggcttaa tagtttctta atacaaagcc tgtaaaacgt caggataact
    19081 tcagaggtcg tcggtaattt atgatgaaca gcaccaataa acttagtgtt attattccgt
    19141 tatataatgc gggcgatgat ttccgcactt gtatggaatc tttaattacg caaacctgga
    19201 ctgctctgga aatcattatt attaacgatg gttcaacgga taattctgtt gaaatagcaa
    19261 agcattacgc agaaaactat ccgcacgttc gtttgttgca tcaggcgaat gctggcgcat
    19321 cggtggcgcg taatcgtggg attgaagtgg caacgggcaa atatgtcgct tttgtcgatg
    19381 ctgacgatga agtctatccc accatgtacg aaacgctgat gaccatggcg ttagaggacg
    19441 acctcgacgt ggcgcagtgc aacgctgact ggtgttttcg tgaaacggga gaaacctgga
    19501 aatccatccc cagcgatcgc cttcgctcaa ccggcgtatt aaccggcccg gactggctgc
    19561 ggatggggct ttcttcgcgc cgttggactc acgttgtctg gatgggggtt tatcgccgcg
    19621 atgttattgt taaaaataac attaaattta ttgccggatt acatcatcag gatattgtct
    19681 ggacaacaga atttatgttt aatgcgctgc gtgcgcgata taccgagcaa tcgttatata
    19741 aatattatct gcataatacg tcagtgagtc ggttgcatag acaagggaac aaaaacctta
    19801 attatcaacg tcactatatt aagattactc gtctgctaga gaaattaaat cgaaattatg
    19861 ccgacaaaat tacgatttat ccggaatttc atcagcaaat aacttacgaa gcattgcgtg
    19921 tttgccatgc ggtgcgcaaa gagccggata ttattacccg ccaacggatg attgccgaga
    19981 tatttacttc cggtat
//
LOCUS       DAJTXQ010000078        19304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000078 DAJTXQ010000000
VERSION     DAJTXQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.078"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..511
                     /gene="csgD"
                     /locus_tag="OO515_002612"
     CDS             <1..511
                     /gene="csgD"
                     /locus_tag="OO515_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6576510.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            516..905
                     /gene="csgE"
                     /locus_tag="OO515_002613"
     CDS             516..905
                     /gene="csgE"
                     /locus_tag="OO515_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6576511.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            930..1346
                     /gene="csgF"
                     /locus_tag="OO515_002614"
     CDS             930..1346
                     /gene="csgF"
                     /locus_tag="OO515_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6576512.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1373..2206
                     /gene="csgG"
                     /locus_tag="OO515_002615"
     CDS             1373..2206
                     /gene="csgG"
                     /locus_tag="OO515_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6576513.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2271..2762)
                     /locus_tag="OO515_002616"
     CDS             complement(2271..2762)
                     /locus_tag="OO515_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6576514.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2864..3418)
                     /gene="ycdY"
                     /locus_tag="OO515_002617"
     CDS             complement(2864..3418)
                     /gene="ycdY"
                     /locus_tag="OO515_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6576515.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3442..4179)
                     /gene="ycdX"
                     /locus_tag="OO515_002618"
     CDS             complement(3442..4179)
                     /gene="ycdX"
                     /locus_tag="OO515_002618"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6576516.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4234..5172)
                     /gene="ghrA"
                     /locus_tag="OO515_002619"
     CDS             complement(4234..5172)
                     /gene="ghrA"
                     /locus_tag="OO515_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6576517.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5406..5493
                     /locus_tag="OO515_002620"
     tRNA            5406..5493
                     /locus_tag="OO515_002620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5440..5442,aa:Ser,seq:gga)
     gene            5538..5674
                     /locus_tag="OO515_002621"
     ncRNA           5538..5674
                     /ncRNA_class="other"
                     /locus_tag="OO515_002621"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5765..6451)
                     /locus_tag="OO515_002622"
     CDS             complement(5765..6451)
                     /locus_tag="OO515_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6576518.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7032..7820)
                     /gene="phoH"
                     /locus_tag="OO515_002623"
     CDS             complement(7032..7820)
                     /gene="phoH"
                     /locus_tag="OO515_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6576519.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8441..9712)
                     /gene="efeB"
                     /locus_tag="OO515_002624"
     CDS             complement(8441..9712)
                     /gene="efeB"
                     /locus_tag="OO515_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6576520.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9718..10845)
                     /gene="efeO"
                     /locus_tag="OO515_002625"
     CDS             complement(9718..10845)
                     /gene="efeO"
                     /locus_tag="OO515_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6576521.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10903..11733)
                     /locus_tag="OO515_002626"
     CDS             complement(10903..11733)
                     /locus_tag="OO515_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6576522.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12399..13907)
                     /gene="putP"
                     /locus_tag="OO515_002627"
     CDS             complement(12399..13907)
                     /gene="putP"
                     /locus_tag="OO515_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6576523.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14329..18291
                     /gene="putA"
                     /locus_tag="OO515_002628"
     CDS             14329..18291
                     /gene="putA"
                     /locus_tag="OO515_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6576524.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18331..18969)
                     /gene="rutR"
                     /locus_tag="OO515_002629"
     CDS             complement(18331..18969)
                     /gene="rutR"
                     /locus_tag="OO515_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6576525.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         4924 a         5131 c         4689 g         4560 t
ORIGIN      
        1 ggacgatata tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct
       61 tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct
      121 aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt
      181 tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg tcctgcgcgg
      241 cgaatgctac tttacgcaaa agcttgccag ctacttgatt acgcattcag gtaactatcg
      301 ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataaact
      361 gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt
      421 taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt
      481 ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg
      541 tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg
      601 gattgctaac tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg
      661 ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat
      721 ggggaagctg gatcactata acggtcaatc aggacgttat tttccagact tttttatttc
      781 cgttgaaaag agacttcgag aaaactgtcg tctttgcact gattcaaact gaagaagcac
      841 taaatcgtcg ccagataaat caggcgttat taagtacggg cgatttggcg catgatgaat
      901 tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca
      961 tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact
     1021 ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt
     1081 ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg ttagataact
     1141 ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt aataccggta
     1201 aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat
     1261 tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt
     1321 tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg
     1381 cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga
     1441 agccgccaga ccgacattaa tgcctcgagc tcagagctac aaagatttga cccatctgcc
     1501 agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt
     1561 taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca cggcaatgct
     1621 ggtcacggca ttgaaagatt ctcgctggtt tataccgctg gagcgtcaga gtttacaaaa
     1681 cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa aacggcacag ttgccattaa
     1741 taaccgaatc ccgctgcaat ctttaacggc ggcaaatatc atggttgaag gttcgattat
     1801 cggttatgaa agcaacgtta aatcgggcgg tgttggggca agatattttg ggatcggtgc
     1861 agacacgcaa taccagctcg atcagattgc cgtgaacctg cgtgtcgtca atgtgagtac
     1921 cggtgagatc ctctcctcgg tgaacaccag taagacgata ctttcctatg aagttcaggc
     1981 cggggttttc cgctttattg actaccagcg cttgctggaa ggggaagtgg gttacacctc
     2041 aaacgaaccc gttatgctgt gtctgatgtc ggctatcgaa acaggggtca ttttcctgat
     2101 taatgatggt atcgaccgtg gtctgtggga tttgcagaac aaagcagaac ggcagaatga
     2161 cattctggtt aaataccgcc atatgtcggt tccaccagaa tcctgacaga taaaaatgcc
     2221 ggaattaata atccggcatt ttactgttta agcgaaagcg tgagtgcccc tcacgcttta
     2281 tttttgattt cctgctcacg gtgagccgtc tttgcactac gcgccgccag ccacaggccg
     2341 ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca gcgaaggtaa caccagtttc
     2401 cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa
     2461 agcagcagct gtttggcctg aatacacatc agaaacgcca caataccggt tatgacataa
     2521 ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa taatgaccat cgcccacacc
     2581 acgccactaa gcagggttgc agcggagatc gccagccctt tcaacccacc ttgcgggcag
     2641 gcaaagtagg ccgtacagcc caagaagccc gcccagctta gtaagcccag ggaaacagcc
     2701 acccatcccc agataccgga gagaatgcct gttgtgattg caatagagag aagtatgttc
     2761 atgccgcgca tgatagcaga aaacgcggcc atgaatgaga tcgagcacac attttatgca
     2821 acaacaaatt atctgttgca gttatgagtg acgatgatca catttactct tcagaatctt
     2881 cttccagctc gtcccacatt gcactaatgg catcacgggt tagcggtgcc atggtgcgcc
     2941 agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa cgcaccacac cagggtaaca
     3001 gatactcact gaacagtgtt tccagtgctt cgctctcatc ttccgttgac tgatcttcca
     3061 gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc tggcgtatcc gccagtggca
     3121 tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt tacgtcctca acccatgcgc
     3181 tacgatatgg cggcactgca cattcatcgc cgataaacaa cgcattgtaa tcggcagaga
     3241 cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa ctcatcctgc tccagtggcc
     3301 agttcgcagc cagtttcccc tcacgaatca gggtaaacag cggcaccagt aaaggatctt
     3361 gcggttggcg gtaatagagc gaacccagca cacgacagag gatagaaaac tcgttcatta
     3421 atatatttcc attactagta attaaaggtc tgcaaattcc gcaatcggtg ccataccgcg
     3481 agattcaagg aagttcagta agcggcgcgg agaaacattc aaaatgcgct ctggcggaaa
     3541 atctaccgcg tcgaggattt taagacactc ttcaaattcc cccatggtaa acgcggtgtg
     3601 agaatccgag cctaatgcca cccaacctcc agcatcacgt accgctgcgg ctaccgcacg
     3661 acagttgtct tcactgccct tacgtgagtg taaaaatgaa gaattattga tttccagcgc
     3721 cacctgatgt ttcgcggctg cttcagcaac ggctttcaca tctatttcat atttgggatt
     3781 tccgggatgg cttattatat gcacattgcc gctggcgata gtggcgatca tcgcttgtgt
     3841 attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga aaaccggcaa taatcagatc
     3901 cagcgagtca aacattttac cgctgcaatc aatttcacca tcaacatttt taatgttagc
     3961 ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc ggccagatac gcatgttaat
     4021 gaagtgccag tgatgcggag catcttccat atccgggcca tgatcggtga tcgcaaaaag
     4081 tttaatgccc ttttgtttgg cctgggcaat gtaatcactc aatgtgctat atgcatgtgt
     4141 gctggcaacg gtatgcatat gaaggtcgac gggatacata actctctcct gtattcaatt
     4201 cccgccaagg atagcaggaa tcctgatgct ttattagtag ccgcgtgcgc ggtcgacttg
     4261 cccgcagacc ctctcccctt tttcgagctg ggcaatggtg cgagaaatgt actccacagc
     4321 ttcagcggga cgggtaatcg cggcgacatg tggtgttatc gtcacgcgtg gatgttgcca
     4381 gagcggactt tcaggcggta agggttcacg attaaaaaca tccaacattg cgcctttaac
     4441 tttgccgcta tccagcgccg cgagcaggtc atcttccaca acatgaacac cacgcgccag
     4501 gttgaggaga tacgcgccat ccggtaattt ttcgagtaat tgttgattaa taatgccgac
     4561 ggtttcaggg gtattcggta acaaattaat caatacccga cattggctca gaaatgcaga
     4621 cagttcttcc cgtccggcaa agctttgcac gccaggccac gatttacggg ttcgactcca
     4681 gcaacgcagc ggaaagcgcc aggtttgcag actctgagca actttactgc ccagtacgcc
     4741 tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat tcaggcagcg gttgccaatg
     4801 cgaactattt tgctggatgc gataatcgtc aaaacgtcga aaccaatgca gcacctgact
     4861 gacagcatat tcctgcattt gctcgcccat accggtatct tccaggcgaa aaagtggaac
     4921 agaagggttc agcatttcag ggtgtgcctg tagcttgctc aaaatagaat caacaccggc
     4981 cccgagtgcg aacaccgctt taagatcgcg ccctgccagc atttcaacag gaggatgcca
     5041 gactaaagca taatcagcag agtcattatc tccgcttttc catgctctga ctcttgcctg
     5101 aggaatagct ttgcgcagtg cctcaatcca ccattgggta tcgaacgttg ggtgataaaa
     5161 gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa tttattcgca cttatcgtta
     5221 tgatctacaa aggccaccag cataacaaat ccgtggtcgg tggcaaaaaa agcagatttc
     5281 gcttattaaa accacacatt gattgaagtt tgaataaacg cgcgattttt tcaaaaagtt
     5341 tgttgacctc aggtcatgat ttccctaaat tagcgcccgt tccagcaaga caggaacgac
     5401 aatttggtga ggtgtccgag tggctgaagg agcacgcctg gaaagtgtgt atacggcaac
     5461 gtatcggggg ttcgaatccc cccctcaccg ccatatttaa agaagagctc gtacgaaagt
     5521 acgggctttt ttttcgtata ttgcacacac cggggggatg agaagccccg accggggttc
     5581 gacaactggc gacagccagt tggacagacc gtgaacgcag tgagcgggct gcccgcaggg
     5641 cgagcgaagc gagtcaatcc ccccctcacc gccatcttta aagaagagct cgtacgaagg
     5701 tacgggcttt ttttcgtata ttgcacacac cggggggatg agaagccccg accgggatac
     5761 ttagttaatg caccggttca ctctttgtcg ccgcgactaa tgggcgaatt ttgtcacgag
     5821 tctcccagac cggaggaatt tcctccaatt tagtgacaga agctcgtaca aaaggcaccc
     5881 gtgcaagagt cataattgta tccaaccaac ggtcacgaat aatgcgatcc gccctttcat
     5941 gactcgggtc atccagctca ataacacaga ctattttatt gttcttcttt gaaaccagaa
     6001 cataatccat tgccatgcca ttataaattg caaagaaacg ctttctttct tcttcgctaa
     6061 actcaggttg ttctggaaag ttgatcaagc gccccagaga gacctggcaa tggatatgac
     6121 aataatgtcc aaaccatttt tccagattat cgaaatacat tttttcacaa cgcgtgatta
     6181 accactcttt tctttccaat aaaatacctt cggcaaccat tccctgaaat aactcatctc
     6241 gctcttgcca acgagagtca cgataggttt tctgcggtat atagctcctt gtcgacgcct
     6301 tagcggcagg ggaagttgca gatgaaggga cgggttgcga tttagcgatg attaccgatg
     6361 aggcaacttt tcggagtgtc aattttctgg caacagcaaa accggcgata aatgcaattg
     6421 tcaaacaagt tacaaaagcc ataaagctca taattaattt tcacctatcc atgttgatta
     6481 tatttcatta ccaatgtaac tcgctgcagc gtgttacatg ccaacaatgc aacgatcaat
     6541 atatagttag tatcatgttt tgattattac aaccatccct atttagccat tgcggcttgc
     6601 cttccacaaa aaaataacaa cacacaatat tagcaaagaa ttaattaaga ttaaccttac
     6661 atttataata ttatccctaa agaataaatc agaggaatat attcggtgtt aaacagtcag
     6721 tcaaagtata gcccattgtt gattaaacct cataagcaaa attggcacca gatctcctgt
     6781 acaggttgaa tggccgaaaa aagataggca gatgctcatt tttttggctt ctggaagaaa
     6841 aactctccgc gctggcagta ttacactggt ctgatgctat gacgaaaaat gcctggtaag
     6901 agtatctaaa ccagactcca gttcagctta tcaacacgct caaagtcgcc cccctgaacg
     6961 ctaacctgaa gtgaaaatct gttgaaataa aacaaagccc ggttcgcccg ggctctgcac
     7021 cgataacaca cttaactgta ggcatgcagc gtacgttggc aaagtgccga acgtacgcag
     7081 tcctctttac cgaaccggac gatcccaacc atttcatctt cttcgaaacg ttccagcgcg
     7141 tcacttaatc cggagcacac gccgcgaggc aaatcgcatt gcgtgatatc accgttgacg
     7201 ataaccgtca cgttctcccc gaggcgggtt aaaaacattt tcatttgcgc ggcagtcaca
     7261 ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg tacgtccacg catataggcg
     7321 aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc agtactgcat aaaggaagcc
     7381 cctaagcgcc ggaccagcac gtcgtagacc gggcgaaaat agggagcaaa cttttctgcg
     7441 atatctccag gtaagaagcc aagatcttca tcggcttgca gaactggacg ggtgacgata
     7501 atcctgtcga catccttatg tatcagggcc tctgccgctt ttgctgcgct gatccaggtt
     7561 tttccgcccc cggcttcgcc cgtggcgaat atcagctgct tactctcaat agccttcaga
     7621 tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgaag tatcgcgact gtcgcgggcc
     7681 ataccaatgg cttctacgcc gcccatctgc acaagcgagg tgaccgattc ttcttcacgc
     7741 tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg cctcgcgacg agctttgatc
     7801 actgcttttt gtcttcccat agagagcacc ttgagttgtt tgtattcatc acacgcgccg
     7861 ttggcagcgc gattatgcgc acgaacatca gagggttggc ttccttgtaa gccatagttt
     7921 gcttttggat aaaatgccga aaaacggcta cgcgcaccgt ttacggcgtc ggtaacacat
     7981 gaaaagaaag gatgaggttg aaaatgcaaa gtgacgagat gactaccgga ggagaaaact
     8041 ccgcgagtgg tggcgcgttg attatctaaa acatgtccag tacaggacgt taccatccgc
     8101 gatctccata gtgactgact atcactgccg ggaacttccg ctgctactta ataagtacaa
     8161 cagatctcgc atttattgca acaatatatt tacttatatt taactataaa acactatttc
     8221 agtgacatta gtttctactg gaaagatgac agagtgatga cagtgatgaa aaaagctgtg
     8281 tgctttcagc aggatttgca tcaggcatca gtgcacgatc gccggatgcg gcgtgaacgc
     8341 cttatccagc ctaccaaacc cgcactgatt gtaggcatga taagacgcgc aagcgtcgca
     8401 tcaggcatct gcgcacaaat gccttatccg cctaaaaaca ttaaacccgc aataacgcgc
     8461 ttccgagata atcgttcgcg tccttcactc ccggcagcga aaaaaaataa ccgccgccga
     8521 taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc
     8581 ctttttccag atcgtgttgg tagcagacaa acagcaaccc catatccagt tgtccggagt
     8641 tggtgacccc caacgaataa ctgtagccgc gacgcagcat caggctggac tcactctccg
     8701 ccgtgcgggg attcgccagc cggatatggc tgtccagcgc aatcaccttc ccttccgggt
     8761 cgctggcgta atcaggcaca tcatgctcat gctgcattcc cagcggcgca ccggtttgct
     8821 tatcacggcc aaaaatcgtc tgctgttctt tcagcggcgt tctgtcccaa aattccactc
     8881 gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgca ggctcctgct
     8941 gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta tcgggattgg
     9001 cagtgccgtc tttgaaaccc agcaaattaa tcggcgtctc tttgccttta ctacgcgaca
     9061 cgtgatcgga aataaaccct tcccgcttcc agcgcacact gagcaaatcc ggcgtgtgtt
     9121 tgatgatatc gcgcagcgca tggataaccg tgtcctgggt gttggcgcaa atctgtagca
     9181 acacatcacc atgacataac gccgcatcca gcgagtcgtt ggggaaacgc gtcatcttct
     9241 gcagcttttt tggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca
     9301 ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga
     9361 gtggtggcag gcgcggattt ggcgtttctg gtgctgctcc gccctgagtc agaaaagcaa
     9421 aacgctgagt caacaagcga aacaaccgct caagatcggc tttatcgctg gcaagcacat
     9481 caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac
     9541 cataaaacgg ctgtttctca ttgcgtgcat ccggtgaaag cgtgcccggt gcactttgcg
     9601 ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagcgcacca atccctttca
     9661 gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc tttatactgc ataacgctta
     9721 atccagtccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag taatcggtcc
     9781 tttcagtgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt
     9841 acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccagcag
     9901 ttccgggttg gctttttgca gttgtggacg cagcaaatcg acaattttct gcgagccttc
     9961 aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctaat
    10021 tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt ttgaaggtgg
    10081 gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tctgtataaa gctgctcagc
    10141 gtactgatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa
    10201 accagtgaat tttgggtcgg cggctttttg ctcgtaatca tcttcacggg cgtcaatgct
    10261 gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt
    10321 cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc
    10381 tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat atgcagtaat
    10441 tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt
    10501 gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt attcgccagg
    10561 ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac
    10621 caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt tctgaataat
    10681 gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct tatccgtcac
    10741 ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag aaaacagcgc
    10801 agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct
    10861 ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc
    10921 tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga gataaataaa
    10981 ccagacggcg acttcgctga cgctcggtgc ttcctgatag ccaaaaatcc cttccatcag
    11041 cgtgccaaac agcgagtgag ttgagagcac tgcgctcata tcgaaggcga tttcctgaaa
    11101 gtggttccac aatccggctt catgaaatgc gcgaatggca ccagcagcca gccctgcggc
    11161 gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc
    11221 gccccagtag agcaggaagc ctagcaccac ggcagtagca agaccgagca ttgcacccag
    11281 cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga ccgactccag
    11341 cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc
    11401 acgctgcaat gcgctatcga ctgcctgctc cagttgtact ttgacgttgc gcgacacttt
    11461 gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc
    11521 aaacagttcc tgttcttttt gcggaaattc gccggtggtt tcgttaatga agatacccaa
    11581 gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacgccaa tccatcggcc
    11641 tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc
    11701 ttcgcgcaac ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga
    11761 gtcaatccat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac ggcaaagaaa
    11821 aatacaagcg gaatgtttat aaaatgatgt tggaatgtaa acaaagagag gtaataccag
    11881 gggatataaa cgattattcc aggtacaaag atgggattgt ttacaacgag ccatgtgttc
    11941 ttgccagatg ccgcgtaaac gccttatcca gccgacaaaa ctgtccagac tcaatgcatc
    12001 acagcttcca tgtaaaactg ataagcgggt gtatcaagca atttcgtcca tccgcgatta
    12061 tcaaatctaa ctttttgccc ggaacataat aacagcgcgg gtgttggtgg cgtcaaaatc
    12121 gctgaaacgt tccccgaagg ccggggcagc ccacatggat gtgggctgag ggcgcgtctt
    12181 acaggacgtt acctcgcgcc cgacccggta gccgaagggg ataagctgaa ggtaccgcgc
    12241 agcggcgatt ttgttcgcca gagcccgggg gtgcaggggg cggcggcgac tggccgcccc
    12301 ctgcgcgctc cctgcaccat tcaatttata tggcgaggca cattaaggga gcgcaacccg
    12361 ctgctgaagc caaacaaaac cgccgcaggc taagtccctt agttttcctg caaccgtgac
    12421 ggcggagccg aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac
    12481 ggcgctttac ccagcaaact aaacactaca atcccgatac tgccgaagat gaagcccgga
    12541 ataatttcgt acagtcccag ccagccgaac tgcttccaga cgataactgt cagcgcaccg
    12601 atgatcatcc ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgagaacagc
    12661 accaccggac caaacgccgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg
    12721 cggttttccg ggtttgccgc cagcgcaatc gccaccagcg ccaccaccag caccatcaca
    12781 cgtcctaccc acaccagctc tttctggctg gcctgtttac gcagaaacgc tttgtataaa
    12841 tcttcggtaa tcgcactgga gcacaccagc agctggcaac ttaaggttga cattaccgcc
    12901 gccagaatcg ccgacagcag aatcccggca atccacgggt taaacagaat ttgcgccagt
    12961 tcgataaaca cgcgttcagc gttctggttt accgcacctg ctaccgccgg atgctcgtta
    13021 aagtaagcga taccaaagaa gccaacagcc accgcccctg ccaggcaaag gatcatccag
    13081 gtcatactga tacgacgcgc atggacaatg ctgtggtgag aatctgccgc cataaaacgc
    13141 gccaggatgt gcggctgccc gaagtaaccc agcccccaac ccatcaatga gataatggca
    13201 acaaagttca gccctttgag catatccacg ttttcgatgc tcttttgttt gatcacttcc
    13261 agcgagtcac caaagccacc gacactgata atgacgataa ccggcgtcag gatcagggca
    13321 aaaatcatca ggctggcctg tacagtgtca gtccagctca ccgcgaggaa accgccaata
    13381 aaggtgtaaa ggatcgtcga cgcagctccc gccaacagag ccgtttcgta actcatgcca
    13441 aaggtacttt caaacagacg cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag
    13501 aacagcaaaa taaccagcgc ggagataatg cgcaaaatgc ggcttttatc ttcaaaacgc
    13561 ccggtgaaat aatccggcaa tgttaaggcg ttattattgt attcggtatg cacacgcaac
    13621 cgcccggcca ccagcttcca gttgatccac gcgcctaatg tcaggccaat ggcgatccag
    13681 ctttcggaaa tcccggaaag aaaaaccgcg ccaggcaacc ccattaacag ccagccgctc
    13741 atatccgacg cacccgccga taatgccgtc acgaatggcc caagactacg accgcccaga
    13801 atatagtcgt caaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca
    13861 aagatataga cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa
    13921 aaaattatta tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtaaca
    13981 gagtttatgt tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga
    14041 tttaacacac catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc
    14101 actctctcac attttttgcg gttgcacctt tcaaaaaggt taactgccgc agagaaaaag
    14161 tctgagttat tttttaatca ctgtcatatc gatttctttt attaacattt cattcatttt
    14221 taagcttgct acgcaagtca catttaacat ggttgcacaa agttgcaaca tcatggatat
    14281 ttcacgataa cgttaagttg cacctttcag aacaacagga gtaatggcat gggaaccacc
    14341 accatggggg ttaagctgga cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt
    14401 atcgatcgca caccacactg gttaattaag caggcgattt tttcttatct cgaacaactg
    14461 gaaaacagcg atactctgcc ggagctacct gcgctgcttt ctggcgcggc caatgagagc
    14521 gatgaagcac cgactccggc agaggaacca caccagccat tcctcgactt tgccgagcaa
    14581 atattgcccc agtcggtttc ccgcgccgcg atcaccgcgg cctatcgccg cccggaaacc
    14641 gaagcggttt ctatgctgct ggaacaagcc cgcctgccgc agccagttgc tgaacaggcg
    14701 cacaaactgg cgtatcaact ggccgataaa ctgcgtaatc aaaaaaatgc cagtggtcgt
    14761 gcaggaatgg tccaggggtt attgcaggag ttttcgctgt catcgcagga aggcgtggcg
    14821 ctgatgtgtc tggcagaagc gttgttgcgt attcccgaca aagccacccg cgacgcgtta
    14881 attcgcgaca aaatcagcaa cggtaactgg cagtcacaca ttggtcgtag cccgtcactg
    14941 tttgttaatg ccgctacctg ggggctgctg tttactggca aactggtttc cacccataac
    15001 gaagccagcc tctcccgctc gctgaaccgc attatcggta aaagcggtga accgctgatc
    15061 cgcaaaggtg tggatatggc gatgcgcctg atgggcgagc agttcgtcac gggtgaaacc
    15121 atcgcggaag cgttagccaa tgcccgcaag ctggaagaga aaggtttccg ttactcttac
    15181 gatatgctgg gcgaagctgc actgactgcc gcagacgcac aggcgtatat ggtttcctat
    15241 cagcaggcga ttcacgccat tggtaaagcg tctaacggtc gtggcatcta tgaaggtccg
    15301 ggcatttcaa tcaaactgtc ggcgctgcat ccgcgctaca gccgcgccca gtatgaccgg
    15361 gtaatggaag agctttaccc gcgtctgaaa tcactcaccc tgctggcgcg tcagtatgat
    15421 attggtatta acatcgatgc cgaagaggcc gatcgtctgg agatctccct cgatctgctg
    15481 gaaaatctct gtttcgagcc ggaactggca ggctggaacg gcatcggttt tgttattcag
    15541 gcttatcaaa aacgctgccc gttggtgatc gattacctgg ttgatctcgc cacccgcagc
    15601 cgtcgccgtc tgatgattcg cctggtgaaa ggcgcgtact gggatagtga aatcaagcgt
    15661 gcgcagatgg acggccttga aggctatccg gtttataccc gcaaggtgta taccgacgtt
    15721 tcttatctcg cctgtgcgaa aaagctgctg gcggtgccga atctaatcta cccgcagttc
    15781 gcgacgcaca acgcccatac gctggcagcg atttatcaac tggcaggtca gaactactat
    15841 ccgggtcagt acgagttcca gtgcctgcat ggtatgggcg agccactgta cgagcaggtc
    15901 accgggaaag ttgccgacgg taaacttaac cgaccgtgtc gtatttatgc cccggttggc
    15961 acacatgaaa cgctgctggc gtatctggtg cgtcgcctgc tggaaaacgg tgctaacacc
    16021 tcgtttgtta accgtattgc cgacacctct ttgccactgg atgaactggt cgccgatccg
    16081 gtcactgctg tagaaaaact ggcgcaacag gaagggcaaa ctggattacc gcatccgaaa
    16141 attcccctgc cgcgcgatct ttacggtcac gggcgcgaca actcggcagg gctggatctc
    16201 gctaacgaac accgtctggc ctcgctctcc tctgccctgc tcaatagtgc actgcaaaaa
    16261 tggcaggcct tgccaatgct ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt
    16321 aaccctgcgg aaccgaaaga tattgtgggc tttgtgcgtg aagccacgcc acgtgaagta
    16381 gaacaggcgc tggaaagtgc ggttaataac gcgccaatct ggtttgccac gcctccggct
    16441 gaacgcgcgg cgattttgca tcgcgctgcc gtgctaatgg aaagccagat gcagcaactg
    16501 attggtattc tggtgcgtga agccggaaaa accttcagta acgccattgc cgaagtgcgc
    16561 gaagcggtcg attttctcca ctactacgcc ggacaggtgc gggatgattt cgctaacgaa
    16621 acccaccgtc cattagggcc tgtggtgtgt atcagtccgt ggaacttccc gctggctatt
    16681 ttcaccgggc agatcgctgc cgcactggcg gcaggtaaca gcgtgctggc aaaaccggca
    16741 gaacaaacgc cgctgattgc cgcgcaaggg atcgccattt tgctggaagc gggtgtaccc
    16801 cctggcgtgg tgcaattgct gccaggtcag ggggaaaccg tgggcgcgca actgacgggt
    16861 gatgatcgcg tgcgcggggt gatgtttacc ggttcaaccg aagtcgctac gttactgcag
    16921 cgcaatatcg ccagccgcct ggacgctcag ggtcgcccta ttccgctcat cgctgaaacc
    16981 ggcggcatga acgcgatgat tgtcgattct tcagcactga ccgaacaagt cgtcgtggat
    17041 gtgctggcct cggcgttcga cagtgcgggt cagcgttgtt cggcactgcg cgtgctgtgc
    17101 ctgcaagatg agattgccga ccacacgttg aaaatgctgc gcggtgcaat ggccgaatgc
    17161 cggatgggta atccgggtcg cctgaccacc gatatcggtc cggtgattga tagcgaagcg
    17221 aaagccaata ttgagcgcca tattcagacc atgcgtagca aaggccgtcc ggtgttccag
    17281 gcggtgcggg aaaacagcga agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg
    17341 acgctgatcg aactggatga ctttgccgaa ttgcacaaag aggtctttgg tccggtgctg
    17401 catgtagtgc gttacaaccg taaccagcta ccagagctga tcgagcagat taacgcttcc
    17461 ggctatggtc tgacgcttgg tgtccatacg cgtattgatg aaaccatcgc ccaggtcact
    17521 ggctcggccc atgttggtaa cctgtatgtt aaccgtaata tggtgggcgc agtggttggt
    17581 gtgcagccgt tcggcggcga agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc
    17641 tatctctacc gtctgctggc gaatcgcccg gaaagtgcgc tggcagtgac gctcgcgcgt
    17701 caggatgcag agtatccggt cgatgcacag ttgaaagccg cattgactca gccgctaaat
    17761 gcactgcggg aatgggcagc aaatcgtcca gaattgcagg cgttatgtac gcaatatggc
    17821 gagctggcgc aggcaggaac acaacgattg ctgccggggc cgacgggtga acgcaacacc
    17881 tggacgctgc tgccgcgtga gcgcgtgttg tgtattgccg atgatgagca ggatgcgctg
    17941 actcagctcg ccgccgtgct ggcggtgggc agccaggtac tgtggccgga tgacgcgctg
    18001 catcgtcagc tagtaaaggc attgccatcg gcagtcagcg aacgtattca actggcgaaa
    18061 gcggaaaata taaccgctca accctttgat gcggtgatct tccacggtga ttcggatcag
    18121 cttcgcgcat tgtgtgaagc cgttgctgcg cgggatggcg caattgtttc ggtgcagggt
    18181 tttgcccgtg gcgaaagcaa tatccttctg gaacggctgt atatcgaacg ttcgctgagt
    18241 gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta aacaggtagc
    18301 cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct caataataat
    18361 ccgctgcacg ttttcaaccg tttgattgaa aaatatctca tcgcgcaacg tcgcgcctgt
    18421 caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc aaatcatgaa
    18481 aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac cggcaatcag
    18541 cgcagatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca ttaacagcgg
    18601 cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat cgcgggagac
    18661 ttccagcttc agacggatgt actcttttat cgccgccagc ggagcgaaat cttcacgaaa
    18721 cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa tatacagcgc
    18781 ctcttttgac ggaaagtaat acagcagatt ggtttttgaa acacccgcca actctgcgat
    18841 ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag
    18901 aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt tcactgcgcc
    18961 ttgcgtcatg cgctctcccc tcttgtagat tggcaacagg atagcaaaac ccccgcccgg
    19021 cctcgacctt ctgcactctc atcgcgcaca gatgcatgtt ttatgtgcaa ctgttttgac
    19081 cgtttagtcc actttttatc agatatttta gataacgtaa tattaacata ttaataacat
    19141 taacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct gaaaaggata
    19201 tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg ttgatgatga
    19261 aaattggcgt attcgtacct attggcaaca acggctggct catt
//
LOCUS       DAJTXQ010000079        19155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000079 DAJTXQ010000000
VERSION     DAJTXQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.079"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            325..891
                     /gene="yqaB"
                     /locus_tag="OO515_002630"
     CDS             325..891
                     /gene="yqaB"
                     /locus_tag="OO515_002630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6576526.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            888..1316
                     /locus_tag="OO515_002631"
     CDS             888..1316
                     /locus_tag="OO515_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6576527.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1389..2945
                     /gene="gshA"
                     /locus_tag="OO515_002632"
     CDS             1389..2945
                     /gene="gshA"
                     /locus_tag="OO515_002632"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6576528.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3095..3610
                     /gene="luxS"
                     /locus_tag="OO515_002633"
     CDS             3095..3610
                     /gene="luxS"
                     /locus_tag="OO515_002633"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6576529.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3674..5212)
                     /gene="emrB"
                     /locus_tag="OO515_002634"
     CDS             complement(3674..5212)
                     /gene="emrB"
                     /locus_tag="OO515_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6576530.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5229..6401)
                     /gene="emrA"
                     /locus_tag="OO515_002635"
     CDS             complement(5229..6401)
                     /gene="emrA"
                     /locus_tag="OO515_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6576531.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6528..7058)
                     /gene="emrR"
                     /locus_tag="OO515_002636"
     CDS             complement(6528..7058)
                     /gene="emrR"
                     /locus_tag="OO515_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6576532.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7149..7484)
                     /gene="ygaH"
                     /locus_tag="OO515_002637"
     CDS             complement(7149..7484)
                     /gene="ygaH"
                     /locus_tag="OO515_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6576533.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7474..8211)
                     /gene="ygaZ"
                     /locus_tag="OO515_002638"
     CDS             complement(7474..8211)
                     /gene="ygaZ"
                     /locus_tag="OO515_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6576534.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8335..9519)
                     /locus_tag="OO515_002639"
     CDS             complement(8335..9519)
                     /locus_tag="OO515_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6576535.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9810..10802)
                     /gene="proX"
                     /locus_tag="OO515_002640"
     CDS             complement(9810..10802)
                     /gene="proX"
                     /locus_tag="OO515_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6576536.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10860..11924)
                     /gene="proW"
                     /locus_tag="OO515_002641"
     CDS             complement(10860..11924)
                     /gene="proW"
                     /locus_tag="OO515_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6576537.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11917..13119)
                     /gene="proV"
                     /locus_tag="OO515_002642"
     CDS             complement(11917..13119)
                     /gene="proV"
                     /locus_tag="OO515_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6576538.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13475..14434)
                     /gene="nrdF"
                     /locus_tag="OO515_002643"
     CDS             complement(13475..14434)
                     /gene="nrdF"
                     /locus_tag="OO515_002643"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6576539.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14444..16588)
                     /gene="nrdE"
                     /locus_tag="OO515_002644"
     CDS             complement(14444..16588)
                     /gene="nrdE"
                     /locus_tag="OO515_002644"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6576540.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16561..16971)
                     /gene="nrdI"
                     /locus_tag="OO515_002645"
                     /pseudo
     CDS             complement(16561..16971)
                     /gene="nrdI"
                     /locus_tag="OO515_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(16968..17213)
                     /gene="nrdH"
                     /locus_tag="OO515_002646"
     CDS             complement(16968..17213)
                     /gene="nrdH"
                     /locus_tag="OO515_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6576541.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17461..17790)
                     /locus_tag="OO515_002647"
     CDS             complement(17461..17790)
                     /locus_tag="OO515_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6576542.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17942..18286
                     /locus_tag="OO515_002648"
     CDS             17942..18286
                     /locus_tag="OO515_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6576543.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18323..18772)
                     /gene="alaE"
                     /locus_tag="OO515_002649"
     CDS             complement(18323..18772)
                     /gene="alaE"
                     /locus_tag="OO515_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6576544.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4806 a         4831 c         4952 g         4566 t
ORIGIN      
        1 tacgaaccga gcggtcggag gttcgaatcc tcccggatgc accatattct ccgtaacctt
       61 cagcaatgaa ggtatcgaag agatagcggt attaaccgca ggcaccagga aggataacgt
      121 tgcgccagca acggcccgaa gggcgaacgt cagtgagtca tcctcccgga tgcaccatct
      181 tctccaaaaa atttccttca aaaatatctc atttctaaat aactccttta tccatttcct
      241 gaattttctt ctccttcgcc atcagcgaca aaatcaagca attacgctaa agtagcgtca
      301 tatttttcga cttgcgagaa gaccatgtac gagcgttatg caggtttaat ttttgatatg
      361 gatggcacaa tcctggatac ggagcctacg caccgtaaag cgtggcgcga agtattaggg
      421 cactacggtc ttcagtacga tattcaggcg atgattgcgc ttaatggatc gcccacctgg
      481 cgtattgctc aggcaattat tgagctgaat caggccgatc tcgacccgca tgcgttagcg
      541 cgtgaaaaaa cagaagcagt aagaagtatg ctgctggata gcgtcgaacc gcttcctctt
      601 gttgatgtgg tgaaaagttg gcatggtcgt cgcccaatgg ctgtaggaac ggggagtgaa
      661 agcgccatcg ctgaggcatt gctggcgcac ctgggattac gccattattt tgacgccgtc
      721 gtcgctgccg atcacgtcaa acaccataaa cccgcgccag acacattttt gttgtgcgcg
      781 cagcgtatgg gcgtgcaacc gacgcagtgt gtggtctttg aagatgccga tttcggtatt
      841 caggcggccc gtgccgcagg tatggacgct gtggacgttc gcttgctgtg agtgaagcgt
      901 tatcgctttt ctcgttgttt gccagtagtt ttctcagcgc tacactatta cccggcaact
      961 ccgaagtcgt gctggtggca atgttgcttt ccgggatcag tcatccctgg gttttagttt
     1021 taacagcaac aatgggtaat agccttggag ggttaactaa cgttatcctt gggcgtttct
     1081 ttccattgcg taaaacatcg cgctggcaag agaaagctat cggctggctg aaacgctatg
     1141 gtgcagtcac gctattatta agctggatgc ccgtggttgg cgatttactg tgtctgttag
     1201 cgggatggat gcgcatctcg tggggaccgg taatcttttt tttgtgcctt ggtaaagcgt
     1261 tacgctatgt tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg cactaattgt
     1321 aggcctgcac atatggtcac cattacagtt atgctaatta aaacgatttt gacaggcggg
     1381 aggtcaattt gatcccggac gtatcacagg cgctggcctg gctggaaaaa catcctcagg
     1441 cgttaaaggg gatacagcgt gggctggagc gcgaaacttt gcgtgttaat gctgatggca
     1501 cactggcaac aacaggtcat cctgaagcat taggttccgc actgacgcac aaatggatta
     1561 ctaccgattt tgcggaagca ttgctggaat tcattacacc agtggatggt gatattgaac
     1621 atatgctgac ctttatgcgc gatctgcatc gttatacggc gcgcaatatg ggcgatgagc
     1681 ggatgtggcc gttaagtatg ccatgctaca tcgcagaagg tcaggacatc gaactggcac
     1741 agtacggcac ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg ctgaaaaatc
     1801 gctacggcgc gctgatgcaa accatttccg gcgtgcacta caatttctct ttgccaatgg
     1861 cattctggca agcgaagtgc ggtgatatct cgggcgctga tgccaaagag aaaatttctg
     1921 cgggctattt ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt ccttatctgt
     1981 ttggtgcatc tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg tcgctgccgt
     2041 ttgagaaaac cgagtgcggt atgtattacc tgccgtatgc gacctctctt cgtttgagcg
     2101 atctcggcta taccaataaa tcgcaaagca atcttggtat taccttcaac gatctttacg
     2161 agtacgtagc gggccttaaa caggcaatca aaacgccatc ggaagagtac gcgaagattg
     2221 gtattgagaa agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag attgaaaacg
     2281 aactgtatgc gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca ccttctgatg
     2341 cgctgttacg tggcggcatt gaatatattg aagtgcgttc gctggacatc aacccgttct
     2401 cgccgattgg tgtagatgaa cagcaggtgc gattcctcga cctgtttatg gtctggtgtg
     2461 cgctggctga tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc gttaactgga
     2521 accgggtgat ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc tgcgaaaccg
     2581 cgcagttccc gttactgcag gtgggtaaag atctgttccg cgatctgaaa cgcgtcgcgc
     2641 aaacgctgga tagcattaac ggcggcgacg cgtatcagaa agtgtgtgat gaactggttg
     2701 cctgcttcga taatcccgat ctgactttct ctgcccgtat cttaaggtct atgattgata
     2761 ctggtattgg cggaacaggc aaagcgtttg cagaagccta ccgtaatctg ctgcgtgaag
     2821 agccgctgga aattctgcgc gaagaggatt ttgtagccga gcgcgaggca tctgaacgcc
     2881 gtcagcagga aatggaaacc gctgataccg aaccgtttgc ggtgtggctg gaaaaacaca
     2941 cctgacagaa aagaaaaagg ccactcgtga gtggccaaaa tttcatctct gaattcaggg
     3001 atgatgataa caaatgcgcg tctttcatat actcagactc gcctgggaag aaagagttca
     3061 gaaaattttt aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata gcttcacagt
     3121 cgatcatacc cggatggaag cgcctgcagt tcgggtggcg aaaacaatga acaccccgca
     3181 tggcgacgca atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag aagtgatgcc
     3241 agaaagaggg atccataccc tggagcacct gtttgctggt tttatgcgta accatcttaa
     3301 cggtaatggt gtagagatta tcgatatctc gccaatgggc tgccgcaccg gtttttatat
     3361 gagtctgatt ggtacgccag atgagcagcg tgttgctgat gcctggaaag cggcaatgga
     3421 agacgtgctg aaagtgcagg atcagaatca gatcccggaa ctgaacgtct accagtgtgg
     3481 cacttaccag atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca ttctggaacg
     3541 tgacgtacgc atcaacagca acgaagaact ggcactgccg aaagagaagt tgcaggaact
     3601 gcacatctag tcagtaaact atcttcacaa ttaattgaaa aaagccagtt caaatgaact
     3661 ggcttagttg tacttagtgc gcaccgccgc caccgccacc tgcgccaaat ggcggtttag
     3721 caaaccacac cagccccagc agtacgagga atatcccggc tgacatccag aagatctcat
     3781 tggcggaaat aatcagcccc tgattggtga tctgctgggc aatccagcct gatgcctgct
     3841 gttgcgtcat cccaagccct tccagttgac tgtacatcgc ctgggcattc gggttgaacg
     3901 ggtttaccga ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc cacatggtcg
     3961 tggttatcga cgtgccgata gaccccgcca gcgttcgcgt aaagttagag aggctcgatg
     4021 ccgccgccag tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc ggcataaaga
     4081 agcaggccac cgcaaacccc tggataaact gcggccaggc cgacgcgcca aaatccatac
     4141 ctggttcaaa ggtataggca cgccagtaga agcagacggc atacataata aagctgaagg
     4201 ttaccagccg ccgcatatcc agtttatgcg cgaagcggcc gataatcggc gacaggatca
     4261 ccggaataat ccctaccggc gcagaggcca aacctgccca ggtcgccgtg taaccgtaga
     4321 cctcctgcaa caactgcggc agcagaacaa tagcgccgaa gtagagcata tacgcgaggc
     4381 tgatacacaa gcagccgatg gtgaagttgc gcgacttaaa caacgacaga tcgactatcg
     4441 ggttatcgtc ggtcagctcc cagacaatca ggaagcagat agccaccacc gccaccacgg
     4501 taaggatgat aatttcctgt gatgaaaacc agtccagctc tttaccgcgg tcgagcataa
     4561 tctgcaggct gccgatacca ataaccagca gtgccagccc cacggcatca atccgccgcc
     4621 gtttggtgcg ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc accgccacgc
     4681 caatcggcac gttgatgaag aatatccagc cccagtggta attatcgctg atataaccgc
     4741 cgaggatcgg gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga
     4801 tcgagcgttt ggctggcggg tagttattca gcaatagact ttgcgaaagc gggatcaacg
     4861 gcccggcgac aatcccctga atcacgcgga agaagatcag catattcagg ctgctggaga
     4921 caccacacgc ccacgacgca atagcaaagg cgatggtgga ccaaaggaac agtttcactt
     4981 ccccgacgcg ctttgccagc cagccggtaa gcgggatcga gatggcattc gccaccccga
     5041 aagaagtgat tacccacgtt ccctggctga gcgatgagcc cagattcccg gcgatagtgg
     5101 ggatcgccac gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg
     5161 caatcgtcat aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca
     5221 cctctggatt agccagcgtt agcttttacg atatcgtcga tcagtttatt gacaggtgcc
     5281 aggctgattt cacgcgcggt acttaccgcg accggtgttg aacgtacttt atttgccagt
     5341 acctgaccgt cacggttagt ggtattgacg ctcaccagtg tggacaaacc aatgcgcagc
     5401 gggtattgct ccagctgttt ctggtccagt tcgatacgca caggtagacg ctgaacgact
     5461 ttgatccagt taccggtcgc attttgcgcc ggaagcagtg agaatgcgct acctgtgccc
     5521 atatccagac caaccacttt accggtgtat ttcacatcat cgccgtaaat atccgtggtg
     5581 atagtgaccg gctgaccgat acgcatattg gcaatctgcg tctctttaaa gttggcatcc
     5641 acccacatat tggtggctgg aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc
     5701 ccaggctgta ccgcacggcg ggagacataa ccggtcatcg gactgacaat acgggtacgc
     5761 tccagcgcca gccaggcgtt acgtacttcg gtggcagcct gttgcacggc aggctgatct
     5821 tccagtttag tccccagaat catcgcctga ttggcattgt attgttgaat agcgacgtcc
     5881 agttgcgcct gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc
     5941 aggttggcat tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt tgcgagggcg
     6001 attttctgca cctcaatatt cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt
     6061 tggcgaacgc tggaagccag tgcagttttg gctttttcaa acgcctggcg agcatctgtc
     6121 gggtcgagag tgaccagcac gtcgccttct tttacaaaat cggtgttatc ggcccagact
     6181 ttcgtcacgc tgccagacac ctgagacata atttgaatct gattccctgc cacgtatgcg
     6241 tcatcggttt cttcgaaatg acgcagtacc aaaaaccaat aaatccctat cgctacggca
     6301 ataattataa agagcaaggt gagaaggagg agcagacgct tacgtttgcc gctcttcttt
     6361 accggttgct gcggggtttg agtctccgca tttgcgctca tattgttctc cacgatcttc
     6421 ttaactcatc ggctgagccg acctgcttgc caaaaaggcc agcatgttgc gatgctggcc
     6481 ggtcattttt tcttttataa atctggattt ttgagcgaga tgacgcgtta gctcatcgct
     6541 tcgagaacca caccgtcttg ttccatctgg tcgagacggg agagcaattt gcgggtgatt
     6601 tgctcgagct gatctttttc tgttgtgctg agcgcggacc agagttgatg caggcagtta
     6661 tgctgcggtg gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc
     6721 aggcagcggc gatcgttatc gctttcacga cgttcgatcc aaccgcgttt ttccagttca
     6781 tcggcaatac gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa ttcagaaggc
     6841 tgaatactgt ggttttcctg agactccagc gtaatcaacg ccataaacaa cgtctcgtta
     6901 atcccctgag ccttcagcat tttattgcgg ttctccagca gcttgctttg catgtgcatg
     6961 caaagacgag tcagaaggat ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat
     7021 tttagcattt gttcaatggg cgtaaacgaa ctatccattt gggtatgacc tcattaattt
     7081 cagccgatat agtaacgaca gtgacaaata aagtaaatgt attgttttag aaaaatgatt
     7141 cttgtgggtt atataatcgc catcactttc caggcgagcc cataggccag cgcactaagc
     7201 agtgttggga tgataatgct gcgtgtttta tagaaactgg cacccagtac cgcgaagccg
     7261 accagcgtgg gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc
     7321 agcagagcgc atatcgaggc gatgccaatg gtgtcgagca aaatacctac cgcgccacgt
     7381 ttggttgggc gggcattacc cacacgcagg cgcagcggca aatagcggaa gcaataattc
     7441 gccgcgccaa ccagtaaccc aggcagcaga acctcatagc tcatcgggcg ctccttgcca
     7501 gaatgcttgg attaacgcag tgaggcagcc acagacaatg cctgccagaa tggcgacggg
     7561 aatagaaaat agcgttacgc ctgcaagggc accaactaac gctgcggtaa cgcaaagaga
     7621 ttgtttgcgc tggaaagagg cgagcaggaa actcataaag agtgccggaa gcataaaacc
     7681 taatgctgct tcaacggcgg gataaccttg cagcaagccg ctgccagaga atgcccctat
     7741 taccgtacca aatacccacg atgaccatga actgaaggca atgccgatca tccagttctc
     7801 gctccagcgg cgattattgc gtaccagttt tgctgttgcg gcggcaaaaa cctcatccgt
     7861 caggccaaac gcccacaggg cggttttcga tttttgcaga cgctgaataa tacggctacg
     7921 cagtgacggg ccatacaaca catggcgaac atccattgcc atgacggtca gtgcagcaac
     7981 ccacaaacta ctcccggctg ccagcatcgc ggtaatgacg aactggctcg cgcctgcata
     8041 aatgatgcag gagaaaaaaa cgctttcgag aggagagaat cccagacggg tcgcattcag
     8101 accgaacgca aaggccaccg gaatataact aataacaatc ggtaaactgt ctttgcatcc
     8161 ttccatgaag gtcgccgaac caggagcagg ctgtggagta gggctttcca tagagtgtac
     8221 gcttaacgat tgttaatatt ataaatagac tgaatgaata tcttaacctt atcagactga
     8281 tgggcttctt aacaccctta taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt
     8341 tgcggcttcc tgacgatgaa aacctcgcca ccagaccagt aagttaacta gggcaatcgt
     8401 cgcgccagcc agacaaacgc cagcccaacc gccatgttgc caggctgagg ctgaaattag
     8461 cgaaccggcg gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca ggcgattacg
     8521 cgcatcaggg tgtattcgat aaattaccgt ctggttagtg atatgcacgc cctgtacggt
     8581 gagatccagc accaggatgc cgataatcag cgccagtacg gaagtgtgac caaaccagat
     8641 cgccagccat gaaagtaata gcagcagcag accgaaagtt gtggtgtggt gcgatttacc
     8701 cttatcggca aaaccgcccg ccggacgagc gcccaacgct ccggcagctc ccgcaagtcc
     8761 aaacagacca atgacaccat cgctgtagtt aaaaggtgga gcggcaagca aaaaggccat
     8821 tgaggtccag agaatgctga agttggcaaa ggtcaggcag cccagcaatg cgcgggtgcg
     8881 gaggatctta tcgctgataa acatgctgaa aacggaacct aacaactgtg ggtagttgag
     8941 gtgggtttct gatttcattt gtggcagacc acgccataat gctagcgcca tcagtgccat
     9001 taacaccgag gcaacccaaa agacggtgcg ccagccgccg aggtttgcca gtaacccggc
     9061 aacggttcgt gccagcaaga tccccaacag cagaccgctc ataatggtgc caaccacttt
     9121 gccgcgttta tccggtgaag ccagcgtcgc tgccagcgga accagaattt gtgccacgac
     9181 tgagaataaa ccggttaatg ccgtaccgag gatcatcatc gccagcgact gactgctggc
     9241 agtgattaac atgccgcctg ccgccagtaa ggtcatcgag acaatcaggc ggcggcgttc
     9301 aaacatatca ccgaggggaa caagaaacag cagacctgct gcatagccca actgcgcggc
     9361 ggtaacaata aagcctgccg aactggcgga aagggaaaag ttacgcgcga tggtgtcgag
     9421 caatggctgg gcgtaatagt tactggctac cgcctgaccg gtggcgatag acatcagcac
     9481 gatcagcgcc gggctaagct catgattagg tttagtcatt atttttgttc atcaggtgat
     9541 gattaagctg gaaatcaata atagcggatg ttgatgaatg ggggcgaaag atagcttgtt
     9601 gaaatgtgcg atgtggtttg taggcatgat aagacgcgcc agcgtcgcat caggcattgt
     9661 gcacagaacg gcggatgcgg cgaaaacgcc ttatccgcca tacggaatga gtgtattttg
     9721 taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaatg gcggatgagg
     9781 caaaaacgac ttatccgccc gaataaaaat tacttctgcg ctgccagcgc ctcattcacc
     9841 cagccatcga actgctgctg gtgggctttg atccaaccat caacatggcc ctgaatatcg
     9901 ccttctgagg ctttgccgtc atgcataatg gcgttctggg cgttaatatc tgccactggc
     9961 aactgcataa tggcaaaaag tttcgctgct gccgggtttt tctcggccca ggctttgttg
    10021 gcaacgatat gcatggtgct gaccgggaag ccataattcg caccattcgg cagtttggta
    10081 tcggcgtttt tatcgcccgg cagtgcggag aacggcacct gcaaccagac cacatctttc
    10141 cctggcttca gctcattact cacccagtac ggcgtccagg tgtaataaaa caccggtttg
    10201 ccctctttgt agcgactgat ggtgtcggcc atcatcgctg cgtagttccc ctgattatgc
    10261 gtcacggtgt tggtcagttc atacgcggca agctggtggt tgatcgcacc ttcgcagccc
    10321 cagcctgggt tacagccggt taaatccgct ttaccgtcgc cgttggtatc gaacagtttg
    10381 gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt tgtactggtc ggcggttttc
    10441 ttatcgatca ggtaaccctg tgccgcgccg ttaacgaata tcccttcaca ataaaatttc
    10501 ttatcgccac cggcagcttc gtacatgttg tcatgcagcg gcgtccagtt cacggcggtg
    10561 aaggttgcat cgccggaagc aagtgaggtg tagccaacgt tgtaatccac ttcgctgggt
    10621 ttattgacgg tataacctaa tttctccagt gcacgactga ccagcagcgt ctggaaggtt
    10681 tcttcagtga tggtgctctg aactggatta acagtaatgc ctttgcccgg cagatcggct
    10741 gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg caaaaagtac gctatgtcgc
    10801 attgttattc ctttttttgg cagggtgata atgcccggcg ttaccgggca agtgcagagt
    10861 tacttaatga atgggcgggt cagcagaccg acagggccag tggtgtacca gcgacggttg
    10921 ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat aatggcgagg
    10981 atcacaatcc cgacgccgcc aacggtggca aggcccatat ccagacgacc gataccgcga
    11041 agtaccatct gacccagccc gccgacggca atcatcgagg cgatgaccac catagaaaga
    11101 gccagcatca gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag cggtaactga
    11161 actttgaaca gcatctggcg cgggctggca ccgaatgagc gcgaggcttc aatcagatcc
    11221 gccggaacct ggttaatccc cagaatcgtc agacggataa tcggcggcag agcaaagatg
    11281 atcgtcacca ctacgcccgg cacgttaccg ataccaaaca gcatgacgat tggtaccaga
    11341 taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg gacgaataat tttcgccgct
    11401 cgcggacttc tcgccagcca tatccccaac ggcaaaccga tgacgataca gaacagcagg
    11461 gcggttaaca ccagcgccag agtcaccatt gcctgcgacc aggcaccgat tgcgccgatg
    11521 gcaatcagcg aaaccagcgt cgccacgccc attccgaccc cggaaatctg ccaggcgatg
    11581 agagcgaaaa cgataatcgc cactggcgct ggcataccca gcagcaattg ctggaaactg
    11641 ttgaggatat aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa atgggtaaca
    11701 acccagtcga tcccttcagt gacccaactg tcgagcggga tcagcgtttt atggaacgga
    11761 tcgagaatat taaaatgctc gacgtttggc gcaggcgtac tggtcagcca gtcagcacca
    11821 ccgccgtcag tcggtgcagt cgtcggtgta ccccaggcgt ctgcggattg cgcggcactg
    11881 tccgccgctg gcgtggtatc ccacggatta ttttgatcag ccattattta ccccctcacg
    11941 atctaaagcg cgcagcagca ttcctttcga aatgatgccg acatactgtt ggtcctcgtc
    12001 gaccacgggc accgcacagg gtgcctgtcc gacatgagag agcaactcgc taagaggcgt
    12061 ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca tcaagacctt gctgctgcgt
    12121 taacgcggtt ttaagcgaat cgatggagac tgcgccgaca aacttattac cgcgttcgat
    12181 aacgtagcca tattcgcgat cttcatcctg caataatttc agtgccgaac gtgggccgaa
    12241 gccaggggtt ttacgaatta agccattcgg tgtccggcgg gcaatatctt tcgcactgaa
    12301 tacctgacta atatcaacgc cacggaagaa ggtacggaca taatcattcg ccggattatt
    12361 gagaatttca tccggtgtgc cgacctgtac cacttcacca ttttgcataa tggcaattcg
    12421 gtcgccaata cgcatggctt catcgagatc gtgggaaata aagacaatgg tgcgctgatg
    12481 tttcgcctgt aattttacca gctcatcctg catctcggtg cgaattaatg gatcgagcgc
    12541 cgagaaggct tcgtccatta ataaaatatc cggattaatc gctaacgcgc gggctaatcc
    12601 cacacgttga cgcatcccgc cagagagttc atccgggtag ctgtgggcat aattttccag
    12661 cccgacctga cgcagtgcat caagggcttt ttcccggcgt tcttcggcat taattccggc
    12721 caattccata ccgaacgcag tattgtccag cacggtcata tgcggcatta aggcaaagga
    12781 ctggaagacc atcgcaatct tttttctgcg cacctcacgg agtttggcat cagatatttt
    12841 ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg ggttcaatca ggcgattgag
    12901 aaggcgtacc attgtggatt tacccgagcc ggataatccc atgatgacaa atatctcgcc
    12961 ttcttcaatg gccagactgg cgtcttttac gccaagcgat agcccagttt tttccagaat
    13021 ttgttctttt gaaagtcctt gttcgatata tttgaatgct cgctgtggat gctcaccaaa
    13081 tattttataa aggtttttaa tttctaattt aattgccatg caatagaaag attcctgtta
    13141 tttgtttatg tcgatatatt acccaatgga aatagttact tttttctacc ctaacatact
    13201 gagaatctga ggcaacccct gatggcaaat gtggcatgaa tattgatgcg agcaaatcgc
    13261 cggaatttcc cgtgatataa gggctgagag caaatcgaga aaagttgata ttttttgtca
    13321 aagatagcga aaaaacgcct gaatattgtg gtttcaggcg aataatgcaa cagagtatca
    13381 gtgtagatca ccacaaaata tttgcgtgat aaaaagctat ttggcgggaa ttatttccct
    13441 gtcgcgggta gtgatatttt tgaaaataac accctcagaa attccagtct tcatcttctg
    13501 tttcaaccgc tttccccatc acataagagg agcctgaacc ggaaaaaaaa tcgtgatttt
    13561 catcggcatt cggcgaaagc gcggcgagga ttgccggatt cacttccgcc atttctgcgg
    13621 gaaataaagg ttcgtagccc agattcatca aagccttatt ggcgttgtaa cagagaaacg
    13681 ctttcacatc gtcagcccac ggggtttcgg cgtacagctc atcggtgtat tgcagctcgt
    13741 tgtcgtagag ttccagcaac aaatcgaagg cgaaactctt caactcttca cgttgtccca
    13801 gagatatctt ttccatgttt ttctgatatt tatagcctat gtagtaaccg tggactgctt
    13861 catcacggat aatcagacgg atcaggtctg cggtattggt cagctttccg cggctggaaa
    13921 aatacatcgg cagccagaaa ccggaataga acaaaaaaga ttcaagaaac acactggcga
    13981 ttttcttttt cagcggatca tcaccgcgat aatgttgctg aataatctga gcttttcgct
    14041 gcaacggtgc gttttcttca ctccaggcgt aggcggcatc gacatctttg gtctggcaca
    14101 gcgtcgagaa aatcgaactg taagagcggg catgaaccgc ttccataaag ctgatattcg
    14161 ataataccgc ttcttcatga ggcgtgagtg catcgggcat cagcgaaggc gcgccgataa
    14221 cattttgcag cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc agttgttgtt
    14281 ctacgacagt taatgtctgc caggcaggaa tatcgttcga cagcggcacc ttttctggta
    14341 gccagaaatt gctggtcagg cgattccaca cctccagatc tttatcgtca gatatcttgt
    14401 tccagttgat ggcgctgata cgtgagagtt tcatagatat tccttaaagt gcacaggaga
    14461 cgcagccttc aatttcagtg ccttccagcg ccatctgacg caggcgaatg taatagagcg
    14521 ttttgatacc tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga gtggtggcgg
    14581 tatcggggaa aaacagcgtc agcgacaaac cctgatcgac atggcgagtc gcttccgcgt
    14641 aggtgtcgat gatcttttct gcgccgattt cgtaagcatc ctgatacagc gccagatttt
    14701 cgttagtcat aaacggggca gggtagtaaa cgcgtcctgt tttgccctct ttgcgtatct
    14761 ctactttcgc cacaatcgga tgaatactcg acgtagcatg gttgatataa gagatagaac
    14821 cggttggcgg caccgcctga agattctggt tgtaaatgcc gtagcgcatc acgtcgtcgc
    14881 gcagttgcgc ccacatctca cgggtaggta acgtaatacc gctgcgggca aacagttcgc
    14941 caactttcgc cgttttcggc tgccagttac cttgcagata ttggctaaaa tattcaccac
    15001 tggcataacg tgactgtttg aacccggcga aggtttcacc gcgttcgcgc gccagcaaca
    15061 tcgaggtacg cagtgcgtgc caggtgatgg catagaaata gagattggtg aaatccagtg
    15121 cttccggcga accataagcg atgccttctc gcgccagata gccgtgtaaa ttcatctgcc
    15181 ccagtccgat ggcgtgtgag gcggcatttc cggcttcgat ggacggcacg ctgcggatat
    15241 gactcatatc tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg cgggcaaaat
    15301 cgggggaatc catggtgtgc gcaatattca acgaacctaa attgcaggag atatcatggc
    15361 ctgtgcgggc atagtcgaga ttctcgtcat actctgaggc acagttgacc tgcaaaatct
    15421 ctgagcagag attactcata tttatgcgcc cggcgacagg gttagcacgg tttaccgtgt
    15481 cttcatacat gatgtaggga tagccggact caaactggat ttctgccagc cgctggaaga
    15541 aatcacgggc gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt tcgtcatagt
    15601 gttcgctgat ggcgatatcg gcaaacggct tgccataaac tcgctcaacg tcatagggcg
    15661 aaaacagcgc catctgcgca ttctcttttg ccagatggaa agtgatatcc gggatcacca
    15721 cgcccagcga cagtgtttta atgcggattt tttcgtcggc attttcccgt tttgtatcga
    15781 gaaaacgcag aatatcggga tgatgagcat gtaaatagac tgcacctgcc ccctgacgcg
    15841 cgccgagttg gttggcatag gaaaatgcgt cttccagcat tttcatcacc ggaattacgc
    15901 cagaagattg attttcaata cgtttaattg gcgcgcccgc ttctcgcaga ttcgacagca
    15961 aaaatgccac gccgccgccg cgtttcgaca gttgcagtgc ggaatttacc gcccggccaa
    16021 tcgactccat attgtcttca atacgcagca gaaaacagga aaccagttcg ccgcgctgct
    16081 gcttaccgca gttgaggaat gtcggcgtgg ctggctgaaa gcgtcctgac agcatttcat
    16141 cggtcagttg caacgccagc gtcgcatcgc cttgtgccag cgtcagcgcc accatcgtga
    16201 ctcgatcggc aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac gtatagctgg
    16261 tgtaaaactt ccatgccccg aggaatgtct ggaaacgaaa accgctggtg tgtgcgtggg
    16321 caaacagcgt aatgacaaag tcgcgagagt agcgattaag aacgctttca tcatagtaac
    16381 cttcgttgac cagccagttc aggcgctgct gctggctact gaaggtcaca ctgttcggac
    16441 gcacatgcgt cgccataaag gcgtcaacgg cctggcggtc tttatcgaac tgaatgcgac
    16501 ctgcgctatc gtagaggtta agcatcgcat tcagcgcgtg gtaatccatc gtttcctgcg
    16561 tcaggcattc tgcggttgtc gttgccaaaa ttcggttact cctttacgaa cgttttcgat
    16621 atcgctttgc gtacccatga gttcaaaacg gtacagccac ggcacgccgc atttccgggc
    16681 aatcacatct ccggcgcggc catacgcctc accaaagttg cgattaccag aagcaataac
    16741 gccgcgaagc aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct atcgtggcac
    16801 cgcgccagcc gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag gctcgtctac
    16861 ctgaatccgt tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta aacgttcgat
    16921 aaaacgctgc gtgttttcgg agctgctgga gaagtagacg agctggctca tgcactggcc
    16981 gcgtgtggcg ctggatgcag acggttaatc atgtccggac ggaaaccaga ccagctaaga
    17041 tcgccagcaa tcactaccgg caactgacga aagccctgag cacgcaacgc ttccgctgct
    17101 tcaggaacgc gatcgacatt aatcatttca aaatcaaagc cccggttttc catcgcccgt
    17161 ttggtggcgt ggcactgaac gcaatcgtta cgagtgtaaa tagttatgcg catgattcgt
    17221 atttccgttt aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct ctctgttgat
    17281 acagagatac tagatgtagt tgaaaaaaga ttcaaccaca caatatatag caaatagtga
    17341 tcgaaattac cctagatatg agcgtgacgg gggaggggga tttttgtgat tcaccaggca
    17401 aaaagaaacc ccgaagacag gcttcggggt taaagacgcg tatttattat catttttgca
    17461 ctacgatttg cgcatgctta acagtgcgcc gataaaaata cctaccgcag ctgctgtacc
    17521 cacgctacac cagggtcttt cacgaacaaa agaatccgcg cagccaacgg catcgcgtgc
    17581 ggcttgctgg acgcgagtac gaccatgcat tcgcgccctg gtttctttca gcaatgcctg
    17641 agctttactg cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg acttcaatac
    17701 agattcgagg ctgtcagcta attgattgac atcattctgg atatcctgca cgccatcatc
    17761 aacgtcgttg cggttcggtc ggttaaacat atgatccccc attagaattc gatgtaattt
    17821 tcagtgtaga ccataatttt cttaactgag gttcatcgcg gctttatgga cttttctgaa
    17881 agcgttgtga atgcgcaatc aggtaaggta ggcccgcggt tgataataac gaggatcaaa
    17941 tatgtattta cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga
    18001 tgtggtaacc cagaaagctt atggttaccg ccgtggggaa aattatgtct atgttaatcg
    18061 cgaagcgcga atgggacgta ccgcactggt aattcatcca acattaaaag aacgtagttc
    18121 gacactggct gaacccgctt ccgatattaa aacttgcgat cattatcaac aatttccgct
    18181 ctatttagca ggcgagcgac acgagcatta cggtatcccg catggcttca gttcgcgtgt
    18241 tgcgcttgaa cgttatttga atggtttatt tggcgaagcc agttaaagac gcgactggcg
    18301 atgccagtcg cgaaaagaag agtcaggctt ttacctgctg gtaatggctg actttaaaca
    18361 gtcggcggca ataatcgagg aagtagccat aaaccgcccc catcaacatc gaaacaacga
    18421 tgtttgaact gaccgccgcc ataatctgat gccagtctgc gcccaccact aacaagatcg
    18481 ccacatacac cggtgactgg aacgtcacat aagccaggat atcagccaga ttttttatcc
    18541 agcccgacgg gctaactttg cgtgccgcgc gcataaatag atcgcggtac ataccgtatg
    18601 gccatgcaat taagatgttc accggaatcg ctaccaatct ggaataaaaa gactgttcga
    18661 agctcattcc ggagaggaaa acttcaatac acatgttcac gacagaacag taaacaacca
    18721 tcgcgaacgt atctgcaact gcatgacgca agcgtgactg cggtgagaac atgctaatgc
    18781 tccttaataa aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc tcattgccat
    18841 ctgcattttc gcatagcgta tatcgctggc tatgactatt caattagtga aaaattttta
    18901 tttagtcagt ttttgtagat aaaatattcc gtaacggctc gttttttgta cattgtggat
    18961 ttttacatgc tgatatgatt tattttcaat taaatcaatt agatatgacc ttgcctttaa
    19021 tttcactaat gaaagtttgg gggtgaggta acgctataag cgtagagaat tcggtgtatg
    19081 tcgtgaccgt gtcatttaca gcatttttga tgccagaaaa tgtacattaa cagcagaaag
    19141 caccagtgag ggggg
//
LOCUS       DAJTXQ010000080        18992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000080 DAJTXQ010000000
VERSION     DAJTXQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.080"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..648)
                     /locus_tag="OO515_002650"
     CDS             complement(<1..648)
                     /locus_tag="OO515_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6576545.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVAT"
     gene            844..1752
                     /locus_tag="OO515_002651"
     CDS             844..1752
                     /locus_tag="OO515_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6576546.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1749..3011
                     /locus_tag="OO515_002652"
     CDS             1749..3011
                     /locus_tag="OO515_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6576547.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3008..3646
                     /locus_tag="OO515_002653"
     CDS             3008..3646
                     /locus_tag="OO515_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6576548.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3651..4427
                     /locus_tag="OO515_002654"
     CDS             3651..4427
                     /locus_tag="OO515_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6576549.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4516..5880
                     /locus_tag="OO515_002655"
     CDS             4516..5880
                     /locus_tag="OO515_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6576550.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADTGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(5974..6966)
                     /gene="rpoS"
                     /locus_tag="OO515_002656"
     CDS             complement(5974..6966)
                     /gene="rpoS"
                     /locus_tag="OO515_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6576551.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7029..8168)
                     /gene="nlpD"
                     /locus_tag="OO515_002657"
     CDS             complement(7029..8168)
                     /gene="nlpD"
                     /locus_tag="OO515_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6576552.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8308..8934)
                     /gene="pcm"
                     /locus_tag="OO515_002658"
     CDS             complement(8308..8934)
                     /gene="pcm"
                     /locus_tag="OO515_002658"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6576553.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8928..9689)
                     /gene="surE"
                     /locus_tag="OO515_002659"
     CDS             complement(8928..9689)
                     /gene="surE"
                     /locus_tag="OO515_002659"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6576554.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(9670..10719)
                     /gene="truD"
                     /locus_tag="OO515_002660"
     CDS             complement(9670..10719)
                     /gene="truD"
                     /locus_tag="OO515_002660"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6576555.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(10716..11195)
                     /gene="ispF"
                     /locus_tag="OO515_002661"
     CDS             complement(10716..11195)
                     /gene="ispF"
                     /locus_tag="OO515_002661"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6576556.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11195..11905)
                     /gene="ispD"
                     /locus_tag="OO515_002662"
     CDS             complement(11195..11905)
                     /gene="ispD"
                     /locus_tag="OO515_002662"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6576557.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11924..12235)
                     /gene="ftsB"
                     /locus_tag="OO515_002663"
     CDS             complement(11924..12235)
                     /gene="ftsB"
                     /locus_tag="OO515_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6576558.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12429..12752)
                     /locus_tag="OO515_002664"
     CDS             complement(12429..12752)
                     /locus_tag="OO515_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6576559.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(12802..13407)
                     /gene="cysC"
                     /locus_tag="OO515_002665"
     CDS             complement(12802..13407)
                     /gene="cysC"
                     /locus_tag="OO515_002665"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6576560.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13407..14834)
                     /gene="cysN"
                     /locus_tag="OO515_002666"
     CDS             complement(13407..14834)
                     /gene="cysN"
                     /locus_tag="OO515_002666"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6576561.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(14836..15744)
                     /gene="cysD"
                     /locus_tag="OO515_002667"
     CDS             complement(14836..15744)
                     /gene="cysD"
                     /locus_tag="OO515_002667"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6576562.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15996..17033
                     /gene="iap"
                     /locus_tag="OO515_002668"
     CDS             15996..17033
                     /gene="iap"
                     /locus_tag="OO515_002668"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6576563.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17301..17594)
                     /gene="cas2"
                     /locus_tag="OO515_002669"
     CDS             complement(17301..17594)
                     /gene="cas2"
                     /locus_tag="OO515_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6576564.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(17591..18514)
                     /gene="cas1e"
                     /locus_tag="OO515_002670"
                     /pseudo
     CDS             complement(17591..18514)
                     /gene="cas1e"
                     /locus_tag="OO515_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18511..>18992)
                     /gene="cas6e"
                     /locus_tag="OO515_002671"
     CDS             complement(18511..>18992)
                     /gene="cas6e"
                     /locus_tag="OO515_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6576565.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4472 a         5144 c         4756 g         4620 t
ORIGIN      
        1 tgtcgccacc tgaccatatt tcgtcgcatc acagaccaaa actcgctggc ggctggcact
       61 ggcaatcgcc cgtttcaccg tgactttatc ttccgctggc gtagaaatcc cccgcacact
      121 ccatgacgat gcagaaataa aagcctgatc aatcatcagg ctgcgcagca tggtcgcagc
      181 ggcttccccg acacaggaac ggttttcccg acacactgca ccgccagtgt gaataattgt
      241 gcaattactg ttgtcgagca agtagttcgc aataacgaaa tcgtttgtga ccacagtcag
      301 tgactccatg tgaatcagat gctgtgctat cgctaacgtg gtcgttcccg catccagata
      361 gatacaactt cccggctgaa caagacttgc cgccagcttg ccaatagccg ctttttgcgt
      421 cattgccagc gcagttttta cctgatgaga aggttcatgc gccacgcgtc ccggagactg
      481 gacgcctccg gacaccagca caacggctcc ctgctgctcc agtttttgta aatcccgacg
      541 aatggtcata tgtgacacat tcattctgtc cgttagttca gcaatactga caatgccttt
      601 ttcagctacc atctcaagga tgatttggcg acgctctacg ggtatcaact tttgctcctt
      661 cctttgtcct gctgacattc tacgctattt gcctgcgaaa cgtgcgcggc gcaactaacg
      721 cttagttcac ataaaataac acacaatgtt aatttatgtg aatcagctca ccatatcgtt
      781 atcttccagc gcttatattc acaatatcaa acaaaatatc acttaaatta acaatgagag
      841 cagatgaaaa cgggatctga gtttcatgtc ggtatcgttg gcttagggtc aatgggaatg
      901 ggagcagcac tgtcatgtgt ccgcgcaggt ctttctacct ggggcgcaga cctgaacagc
      961 aatgcctgcg ctacgttgaa agaggcaggt gcttgcgggg tttctgataa cgccgcgacg
     1021 tttgccgaaa aactggacgc tctgctggtg ctggtggcca atgcggccca ggttaaacag
     1081 gtgctgtttg gtgaaacagg cgttgcacaa catctgaaac ccggtacggc agtaatggtt
     1141 tcttccacta tcgctagtgc tgatgcgcaa gaaattgcta ccgctctggc tggattcgat
     1201 ctggaaatgc tggatgcgcc agtttctggt ggtgcagtaa aagccgctaa cggtgaaatg
     1261 actgtcatgg cctccggtag cgatattgcc tttgaacgac tggcacccgt gctggaagcc
     1321 gttgccggaa aagtttatcg cataggtgca gaaccgggac taggttcgac cgtaaaaatt
     1381 attcaccagt tgttagcggg cgtacatatt gctgccggag ccgaagcgat ggcacttgca
     1441 gcccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg tgaccaatgc cgccggaaat
     1501 tcctggatgt tcgaaaaccg gatgcgtcat gtggtggatg gcgattacac cccgcattca
     1561 gccgtcgata tttttgttaa ggatcttggt ctggttgccg atacagccaa agccctgcac
     1621 ttcccgctgc cattggcctc aacagcattg aatatgttca ccagcgccag taacgcgggt
     1681 tacgggaaag aagacgatag cgcagttatc aagattttct ctggcatcac tctaccggga
     1741 gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt taccggcgcg acggatatcg
     1801 ccagttttct ggtggaaaac ggtctaccaa cggtacaaat taacggtgtt ccaacaggta
     1861 aaatgccgga agcaatcgac gcactggtga tcagcctgaa aacgcgctcc tgtccggtgg
     1921 ttgaagccac acagcaatcg ctggcggctc tgagctggtt gcaacagcaa ggttgcaaac
     1981 agatctattt caaatactgc tctactttcg acagtacggc gaaaggtaat attggcccgg
     2041 ttaccgatgc cttaatggat gctctcgaca cgccgtttac ggtcttctct ccggccctgt
     2101 cggtcaacgg acgtacggtt tatcaggggt atttgttcgt aatgaatcaa ctgctggccg
     2161 aatccgggat gcgccatcac ccggtaaatc ccatgaccga cagctatctt ccccgtctgg
     2221 ttgaagcgca atccacaggg cgctgcggcg tcgtttcggc acatgttttc gaacaaggcg
     2281 tggatgccgt tcgtcaagag ctggctcgct tacagcaaga gggctaccgc tacgcggtgc
     2341 ttgatgcgct gaccgaacac catctggaaa ttcagggaga agccttgcgc gatgccccac
     2401 tggtaacggg cggttctggt ctggcgattg gcctggcccg gcagtgggcg caagaaaacg
     2461 gtaaccaggc tcgcgaagca gggcgtccgc tcgctgggcg cggcgtagtg ctctccggtt
     2521 catgctctca aatgaccaac cgccaggtgg cacattaccg tcaaattgca ccagcccgtg
     2581 aagttgatgt ggcacgctgc ctctcaaccg aaactctggc cgcttatgca cacgaactgg
     2641 cagagtgggt tctgggccag gaaagtgtac ttgctccact ggtttttgcc accgccagca
     2701 ctgacgcatt ggcagcaatt caacagcaat acggtgcaca aaaagccagt caggcagtag
     2761 aaactctgtt ttctcaacta gcggcgcggt tagcagcgga aggcgtgaca cgctttattg
     2821 tcgcaggcgg tgagacctcc ggcgtagtca cacagagcct gggcataaaa gggtttcata
     2881 ttggcccaac catttccccc ggcgtgccgt gggtaaacgc actggataag cctgtctcac
     2941 tcgcccttaa atctggcaac ttcggtgatg aagccttttt ttcacgagcc caaagagagt
     3001 ttttatcatg agcgatttcg caaaagtaga gcagtctttg cgagaggaga tgacgcggat
     3061 tgccagttca ttctttcagc gcggctatgc aaccggttcg gctggcaatc tgtcgctgct
     3121 tttacctgac gggaatttac tggcgacacc gacaggttca tgcctgggca atctcgatcc
     3181 gcagcggctt tccaaagtcg ccgcggatgg cgaatggtta agtggtgaca aaccctcgaa
     3241 agaggtgctc tttcatctgg cgctgtatcg caacaatccg cgctgtaaag cggtggtgca
     3301 tttgcacagc acatggtcga cggcgctttc ctgcctgcaa gggctggaca gcagcaacgt
     3361 tattcgtccg ttcacaccat acgtggtgat gcggatggga aatgtcccgc tggtgcctta
     3421 ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg gaactggcag cagacaatca
     3481 ggctttttta ctggcaaatc atggcccagt ggtttgcggt gaaagcctgc aagaagccgc
     3541 caacaatatg gaagagctgg aggaaacggc aaagctgatt tttattctcg gtgaccgccc
     3601 gatccgttat ctgaccgcag gtgaaattgc ggaattaagg agttaatgca atgcctcgtt
     3661 ttgcagctaa tttatccatg atgttcaccg aagtgccttt tattgaacgc ttcgccgcag
     3721 cgcgaaaagc cgggttcgat gctgtggaat ttctgtttcc ctatgactac tccaccctgc
     3781 aaatccaaaa gcaactggag caaaaccatc tgacactggc gctgttcaat accgctcccg
     3841 gagatattaa tgcgggggaa tggggattat ccgcccttcc cggacgtgag cacgaagcac
     3901 acgcagacat cgacctggca ctggaatatg cattggcgct caattgtgaa caagtccatg
     3961 tgatggcagg cgtcgtgccc gctggcgaag acgctgaacg gtaccgggca gtatttatcg
     4021 ataatctccg ctacgctgcc gaccgctttg cgctacacgg caagcgaata ttagttgaag
     4081 cactcagccc cgacgttaag cctcattatc tcttctccag ccagtatcag gcactggcta
     4141 ttgttgaaga ggttgcgcga gataacgtgt ttattcaact agatactttt catgcgcaaa
     4201 aagtagatgg caacctgacg catttaatcc gcgactacgc tgggaaatat gcgcacgtac
     4261 aaattgccgg actacctgat cgacatgaac cggacgatgg agaaatcaac tatccgtggc
     4321 tgttccgcct gttcgatgag gtgggatatc agggctggat cggttatgaa tataaacctc
     4381 gtggcctcac cgaagaaggg cttggctggt ttgacgcctg gcgctaattc gttaaatccc
     4441 taattacaac gtacccatac atccccccat ctacggatgg ggtaggttaa ctataacttt
     4501 cagacagggt ttcccatgtc cacaattaca ttgttatgca ttgcgttagc tggcgtaatc
     4561 atgctgttgc tgctggtcat caaggcaaag gtacaaccat tcgttgctct gctcctcgtc
     4621 agcctgttag tcgcacttgc ggcgggtata ccggcgggcg aagtgggtaa agtgatgatc
     4681 gccgggatgg gcggtgtgct tggttccgtc actattatta ttggtctggg cgctatgctg
     4741 gggcgtatga tcgaacactc tggtggtgca gagtcactgg ctaattattt cagtcgcaag
     4801 ttaggtgaca aacgaactat cgctgcgctg actctggcag cgttcttcct cggtattccc
     4861 gtcttctttg atgtcggctt tattattctt gctccaatca tttacggttt tgccaaggtt
     4921 gccaaaatat cgccactcaa atttggcctg cctgtcgctg ggatcatgct aactgtacat
     4981 gtggcggtac cgccgcatcc aggccctgtc gccgcagcgg ggttactcca cgcagacacc
     5041 ggctggctaa ccatcatcgg tattgcgatt tctattcccg tagggattgt tggctacttt
     5101 gcagcgaaaa taatcaataa gcaccaatat gcgatgtcgg tggaagtact ggaacagatg
     5161 caactggctc cggccagtga ggaaggcgcg acaaaattaa gcgataaaat aaatccaccg
     5221 ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg cgattatcat ggcgggtacg
     5281 gtttccgcaa cactgatgcc gccttcgcat cccctgcttg gtacgctaca gctgatcggc
     5341 tcaccaatgg tagctctaat gattgcgctg gtgctggcat tctggttatt ggctttacgt
     5401 cgcggctgga gcttacaaca caccagcgac attatgggct cagcccttcc cactgcggcg
     5461 gtagtgattt tggttactgg tgctggaggg gtatttggca aagtgctggt ggaatcgggc
     5521 gtcggcaaag cccttgccaa catgctacaa atgattgacc tgcctctgtt accagccgca
     5581 tttattattt cactggcgct gcgtgcatcg caggggtcag caaccgtagc aatcctgaca
     5641 accggcgggt tactctcaga agcggtgatg ggattgaatc cgattcagtg cgtattggtg
     5701 acgctggcag cctgctttgg tgggcttggg gcgtcacata ttaatgactc agggttctgg
     5761 attgtgacca aatatctggg gttgtcggta gcagacggtc tgaaaacctg gacagtgtta
     5821 acgaccattc tcggttttac cggattctta attacctggt gcgtatgggc ggtaatttga
     5881 ccttaataaa aaggtccgat gagcatcgga ccttttattg tgcacagaaa aggccagcct
     5941 cgcttgagac tggcctttct gacagatgct tacttactcg cggaacagcg cttcgatatt
     6001 cagcccctgc gtttgcagga tttcacgcaa acggcgcagg ccttcaacct gaatctggcg
     6061 aacacgttca cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta
     6121 ccccagcaaa ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca gctcgaacag
     6181 ccatttgacg atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc
     6241 tttttcatcg gccaggatgt ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc
     6301 taccgaggta atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc
     6361 cagttgctct gcgatctctt ccgcacttgg ttcatggtcc agcttatggg acaactcacg
     6421 tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg
     6481 ggtttggttc ataatcgccc gttcaatcgt ctggcgaatc caccaagttg cgtatgttga
     6541 gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc ccaggttgcc
     6601 ctcttcgata aggtccagca acgccagacc acgattgcca taacggcggg caatttttac
     6661 caccagacgc aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc
     6721 gcgacgcgca aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc
     6781 aaggtaaagc tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata acagttcctc
     6841 ttcggccaaa tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc
     6901 aactccgttc tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg
     6961 actcataagg tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt
     7021 ccgccgattt atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga
     7081 atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc
     7141 cccgccttca cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc
     7201 aggtaatcat cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttaccg
     7261 gcataaacaa cgcggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata
     7321 tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc
     7381 ggccagcgcc aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt
     7441 gtgcttgctg ttggtaccgt tacaggcgct gtgactgtgg tcgcagttgg cttgttgttc
     7501 ggcaacattt tgttagcact ctgttcaccc gaagactcag aatacgtaat tgtcggttgc
     7561 gacgcaacag caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg
     7621 tcggcctggg taatggcatt tccgccagtg attggcgtac cggaagcatt acccacctgc
     7681 aaggtctgac caacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca
     7741 cggaaatcgt tgccagtaat ccaggcgata tagaaaagtg tgtcgccttt tttcacggta
     7801 taggtactgc cgctataact gcctttcgga atgttcccat actgacggtt atagacgatg
     7861 cgtccgtttt ccatctgtac cggctgctga gctactggct gcactggctg gatttgcggt
     7921 tgttgagtag cctgaatttg tggctgctgt accggctgaa tttgcggttg ctgggctgta
     7981 gacgtcgtcc ccattttcgg cggcggcgta atcaacatac cagaattagt atttgcaggc
     8041 gcattgccat taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc
     8101 agccatagcg aaaccagtga caaagcagca atgcggcgaa cggtgaattt tgggcttccc
     8161 gcgctcattt atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag
     8221 gcaccctact gaacactgga aaagatgttc acgatacgct gacctgcggc aaaataacca
     8281 ggaaaaatcc aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa
     8341 gcgcacggcc tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa
     8401 atactggtgc tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat
     8461 tagcgcagtt ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa acggcgcacg
     8521 tgcctgccaa ccttgccatc catcgccatg acgggttgaa acattatgta aatcaagatt
     8581 tttcaggcgg cgacgtgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg
     8641 ctggacaaga tgcgctagga ttgccgtttg atatcccgaa ccagtgccaa tttccagcac
     8701 ccgcgactgc ggcgtcagct cgagtaattc ggtcattcgc gccaccatat acggctgcga
     8761 aattgtctgc ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc
     8821 ttcatcaacg aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg
     8881 aataccttgc gcacgtaatt gctccagaag tgcttgtacg cgtctgctta ccattgcgtg
     8941 ccaactccca cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa
     9001 tccacatgca gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa atcggtcccc
     9061 ggaccggcat cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga
     9121 tcttgctgcg ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata
     9181 cctttgattt gatccaaggg taaatccgga acgttaatat taagaatacg tccggtgcgc
     9241 agcggctctt tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa
     9301 tgtttatgcc cgtcaagcga gacggccagc gccggaaaac ctaaatgacg gccttccatc
     9361 gcggcggcta ccgtaccgga ataaataacg tcatccccca gattcggccc ggcgttaatg
     9421 ccggacacaa caatgtccgg gcgcggacgc atcagagcat tcacgccaag atagacgcaa
     9481 tcggtcgggg ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg
     9541 gaggattcca gtgtcagaga atttgaagcg ccgctgcggt tacgatcggg ggcgaccacc
     9601 tgaacgtcag caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc
     9661 ccgtcatcat tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttccctgac
     9721 aacgctggtt gcaaaactac ccgccggaag ccagaaacgg atctctacgg tgacgtcatc
     9781 ccaccaattc cagcttaatt gttgcggata cagcaacatc gctctgcgcg cggcttcaac
     9841 tttttcgcgc accagtaaag cttgtaattc agtttctgcg gcgacagctg ccttttcgaa
     9901 tgccagcgct tcacgctgag ttccccattc gccactaccc ggcaatgcgg cggttatcat
     9961 cagctcttta tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg ttgcgacaaa
    10021 ccagctacca cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc
    10081 ttttttgagg cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca
    10141 aaaactccgt ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gctgcgcccc
    10201 ctgcaagttg ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt
    10261 tacgcaaata tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac
    10321 cagggtaaag gcgttacctt tcagcgcgcc taaacgcagc ttgcgcttgt gccgcgcata
    10381 ctccagcacc tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt ccttgcctgg
    10441 cacgcgagcg cataaccact gttccgtaac agcatgtttg tctttttgcc cagcgaagct
    10501 gacttcacgg gcatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt
    10561 gcagccgttt ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc
    10621 caaatcttcc accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg
    10681 tttaccgtgg aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc
    10741 gccaccgctt cacaggcaat cccttcccca cgtccggtaa atccaagttt ttccgtagta
    10801 gtggctttca cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc
    10861 atttgtggaa tgtgcggcaa catcttcggt gcctgagcga taatagtgac atcgacgttg
    10921 cccagggtat aacccttcgc ctgaatacga cgccaggctt cgcgtagcag ctcgcggctg
    10981 tcggcacctt taaatgccgg atcggtatcc gggaacagct tgccgatatc ccccagcgcc
    11041 gccgcgccaa gcaatgcatc ggtcaacgca tggagcgcca cgtcgccatc agaatgcgcc
    11101 agcaatcctt tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca
    11161 aaggcatgta cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc
    11221 gggtgaggta aaactcggcc agtgccaaat cttccgggcg cgtgacttta atgttatccg
    11281 cacggccttc gaccaactga ggatggaatc cgcaatattc cagcgccgag gcttcgtcgg
    11341 taatagtcgc gccttcattt agagcgcgcg tcagacagtc atgtaacagc tcacgaggga
    11401 aaaattgcgg cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat
    11461 ttttgcccgg ttcggcacgt ttcatcgtat cgcgcactgg tgcggctagg atccctcccg
    11521 tgcggctggt ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc agacaaggac
    11581 gagcggcgtc atgcaccaat acccactgcg cgtcgccagc ggctttcaaa cctgccagca
    11641 cggaatcggc acgctcatca ccgccatcta caacggtgat ttgcggatga ttcgccagag
    11701 gaagttgtgc aaaacggcta tcgccaggac ttatggcaat gacgacacgt ttcacccggg
    11761 gatgcgccag cagcgcatgc accgagtgtt caagaatggt ttgattaccg attgagagat
    11821 attgcttagg acattccgtt tgcattcgac ggccaaatcc ggccgccgga accacggcgc
    11881 aaacatccaa atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc
    11941 ccgcagactg tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc
    12001 tggtcatgct gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat
    12061 cgtcaatttc ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt gtagcttgct
    12121 gtgccgccac atcatcattg acgcgggtat agtcatgtat accgttctta ccgaaccaca
    12181 gcgaatactg tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc
    12241 cccctgaaaa acggcatcat catcccatgc atccgaagac gactctacat cctctgttgg
    12301 ggataccgcg acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg
    12361 cgtgcagaac ataatctcat tattagttac ggtttgaatt atgaacagag gagacaagaa
    12421 agtacaaatt agcccagtag ccacataaac agtgcgccaa acataatgcc tactgtcacc
    12481 agggtgaaaa caatactgta gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc
    12541 accactaccg cgacgggcat cagcgccaga aagaaaggcc aggtgtagat aaagaagaac
    12601 agcgtgttag agccataaat caacatcggc atcgccagcg caaataacca ggagataaaa
    12661 ccgaccacgg caccaggcag tgaccatgtg gtttcttcat cctcagtaag gctgtcgtta
    12721 tttgttagtg taatgttatg gctattacgc atatttgatc ctgttacttt gacgaaccgg
    12781 gcatggaaac ccggtggtgt ctcaggatct gataatatcg ttctgtctca acagatctaa
    12841 taattgctgt accaaatttg ttactaattg ttcaccattg agatgaattt ctgccgattc
    12901 aggcgcttcg taaacggaat ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt
    12961 cttatataag ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac
    13021 ttcgataaag cgcccttctc ctacgcgttc acgaaccatc tggcgttcgg cgcggtgtgg
    13081 cgagataaat gcggtcagca ccaccagtcc ggcttcaacc atcaaattcg ccacttcacc
    13141 gacgcgacgg atattctctt tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc
    13201 gtggcgaaca ttatcgccat ccagcagata cgtactgacg ccgagtttat gtaacgcctc
    13261 ctccagcgcc ccggcgaccg ttgatttacc ggacccggag aggccggtaa accacagcac
    13321 tacaccacga tgaccgtggt gtagctcgcg ttgttgcaca gtgaccggat ggctatgcca
    13381 gacgacgttt tcgtcatgca gcgccattat ttatccccca gcaaatcgcg cgcgccccag
    13441 tgcggaaagt ggcgacgaac cagagcattc aattccagtt cgaatgcact gaattcagat
    13501 ggcgcagcag ttgcctggct aactggctcg tgcaccatac cggcacccac ggtcacattg
    13561 ctcaggcgat cgataaaaat cagcccaccc gtcaccggat tttgttgata acgatctaac
    13621 accagcggct cgtcaaaagt gagatccacg aggccgatcc cattcagtgg caggttttca
    13681 acttcacgct gggtaaggtt attaatatca acctgatagc gaatgccatc aacacgcgcg
    13741 cgcgtcttct taccggcaat tttgatgtcg taactctgcc ctggagaaag cggctgttcc
    13801 gccatccata ccacatccac cgacgcgctc tgcaccgccg gtaacgcttc gtctgccgcc
    13861 agcagcagat cgccacggct gatgtcgatc tcatccgtca gcaccagggt gatcgcttct
    13921 ccggcaaagg cttcttcgcg atcaccatca aaagtcacga tccgcgcgac gtttgattcc
    13981 acaccagagg gcagcacttt tacacgttgc ccgacttcca cgcgaccgga tgccagcgtt
    14041 ccggcgtagc cacgaaaatc gagattcggg cgattaacgt actgcaccgg gaagcgcatt
    14101 ggctgagcat ccaccactcg ctggatctcc acggtttcca gcacttcgag cagtgtcgga
    14161 ccgctgtacc acggcatact ttcactttgc gatgccacgt tgtcgccttc cagtgcagag
    14221 agcggcacaa agcggatatc cagattaccc ggcagctgcc cggcaaaggt cagataatct
    14281 tcacgaatac gggtgaacgt ctcttcactg taatccacca gatccatttt gttgatcgcc
    14341 acgaccagat gtttgatccc caacagtgtg gagataaaac tgtgacgacg ggtttgatcg
    14401 agcacgcctt tacgggcatc gatcagtaag atcgccagtt cacatgtcga tgcgccagtc
    14461 gccatattgc gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc
    14521 tcggtagaga aatagcggta ggccacatca atagtgatgc cctgttcgcg ctcagcttgc
    14581 aggccgtcca ccagcagagc cagatccagc ttttcgccct gggtaccgtg acgcttactg
    14641 tcgttatgca gcgatgagag ctgatcttca tagatttggc gagtatcgtg cagcaggcga
    14701 ccaatcagag tgcttttgcc gtcatcgacg ctgccacagg tcagaaaacg cagcaggctt
    14761 ttatgttgtt gcgcaatcat ccaggcttcg acgccgcctt cattggcgat ttgttgtgca
    14821 agtgcggtgt tcatcttaaa aatacccctg acgttttttc agctccatag atcccgcctg
    14881 gtcgcggtca atcacgcggc cctgacgttc actggtggtg gaaaccagca tctcttcgat
    14941 gatttccggc agtgtttgtg catttgactc caccgcaccg gtcagcggcc agcagcccag
    15001 cgtacggaaa cgcaccatcc gttttttaat cacttcgccc ggttgcaggt cgatacggtt
    15061 gtcatcaatc atcatcaaca taccgtcgcg ttccagaacc ggacgttccg cagcgagata
    15121 cagcggcaca atgtcgatat tttccagcca gatgtattgc cagatatcct gctcggtcca
    15181 gttagagagt gggaagacgc ggatgctttc gcctttgtta atttgcccgt tgtagttgtg
    15241 ccacagctcc gggcgctgat ttttcggatc ccagcgatgg aagcggtcac ggaaagagta
    15301 gattcgctct ttagcgcggg atttctcttc gtcacggcgc gcaccaccga aggcggcatc
    15361 aaaaccgtat ttgttcagcg cctgtttcag gccttcagtt ttcataatat cggtatgttt
    15421 cgcgctgccg tgcacgaatg gattaatccc catcgccacg ccttccgggt ttttatgcac
    15481 cagcagctcg cagccgtagg ctttcgccgt acgatcgcgg aactcataca tctcgcggaa
    15541 tttccagccg gtatcgacat gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt
    15601 gcgcgccaaa tgcagcatga cgctggaatc tttaccgata gagtagagca tcaccggatt
    15661 tgagaattct gccgccacct cgcgaataat gtggatgctt tccgcctcca gttgccgcag
    15721 gtgagtaagt cgtatttgat ccataaccgt tcctttgcaa taccgctatt ttcttgccat
    15781 cagatgtttc gactataggg agcgtaagag aacgaatgaa attaccaatt agaatgagta
    15841 gttccttaac ggaataacga tttggcaaag ctaatatcaa aaagtgctta aggcaccgga
    15901 tttctggcgt ttaggaagat ttgaaattgt tttagcgcag cggaagtttc atactatggc
    15961 ggtaaaaaaa tttgcatggt atttaaggac tcactatgtt ttccgcattg cgccaccgta
    16021 ccgctgccct ggcgctcggc gtatgcttta ttctccccgt acacgcctcg tcacctaaac
    16081 ctggcgattt tgctaatact caggcacgac atattgctac tttctttccg ggacgcatga
    16141 ccggaactcc tgcagaaatg ttatctgccg attatattcg ccaacagttt cagcaaatgg
    16201 gttatcgcag tgatattcgg acatttaata gtcggtatat ttatactgcc cgcgataatc
    16261 gtaagagctg gcataacgtg acgggaagta cggtgattgc cgctcatgaa ggcaaagcgc
    16321 cgcagcagat catcattatg gcgcatctgg atacttacgc cccgctgagc gatgctgacg
    16381 ccgatgccaa tctcggcggg ctgacgttac aaggaatgga tgataacgcc gcaggtttag
    16441 gtgtcatgct ggaactggca gaacgcctga aaaatacgcc taccgagtat ggtattcgat
    16501 ttgtggcgac cagcggcgaa gaggaaggaa aattaggcgc tgagaattta ctcaagcgga
    16561 tgagtgacac cgaaaagaaa aatacgctgc tggtgattaa tctcgataac ttaattgttg
    16621 gcgataaatt gtatttcaac agcggtgtaa aaacccctga agcagtaagg aaattaacgc
    16681 gcgacagggc gctggcaatt gcgcgcagtc acggaatagc cgcaacgacc aatccgggtt
    16741 tgaataaaaa ttatccgaaa ggcactgggt gttgtaatga cgcagaaata ttcgacaaag
    16801 cgggcattgc tgtactttcg gtggaagcga ctaactggaa tcttgggaat aaggatggtt
    16861 atcagcaacg cgcaaaaaca gccgcattcc ctgcgggaaa tagctggcat gacgtaagac
    16921 tggataatca acagcatatt gataaggcac ttcctggaag aatagaacgt cgctgccgtg
    16981 acgttatgcg gataatgcta cctctggtga aggagttggc gaaggcgtct tgatgggttt
    17041 gaaaatggga gctgggtgtt ctaccgcagg ggcggggaat tctaagtgat atccatcatc
    17101 gcatccagtg cgcccggttt atccccgctg gcgcggggaa ctcttactgc ttggtatgcg
    17161 gaatcacacc ctgaacggtt tatccccgct ggcgcgggga acacactaag catacataac
    17221 tgtttttaaa caaatttatt ccgcatcaac aatctaccaa ctaaattaaa acatttcctt
    17281 atttttaaag aacggataac ttattgatta tcaacaggaa gaaaagaaac caaacgtaac
    17341 ccatccaaat ccaccggaat acgtctgttt tctccccagg tctgaaattc aaaacccgac
    17401 tcgatattgg tcgcccaggc catcaccaca tttccgcaac cagccagttg ggtaatttgc
    17461 tgccagatca tctcccgaat acgttttgat gtatcaccaa catacacacc ggcacgcact
    17521 tccagtagcc agattgcgag ccgtccacgt aagcgcggag ggacattttc tgtaacaacc
    17581 acgaccatgc tcatccgccg cgcccccggt gaccactatc acccagcgtt tcaggttcag
    17641 ggatggcagg cggtaacata tccggcgcgg gttgtggtgg ttcaatttca cctgcagcaa
    17701 ggacttcctc aattaacggt attaatttgc ccgttaactt agtgctacgg aaaatatcgc
    17761 gacaggctaa tctgacttct ttatcaggtt ctgcgggttg cctcgctgct atttcaaatg
    17821 cctttggcac aaccgaatca aatttaatga tatcggctat gtcataaaca aatgaaagcg
    17881 gtttgccact atggataaac ccaatagcgg gcgcatatcc cgcggctaat actgccgctt
    17941 cagatatacc gtacagacat gatgtagcag cactgatgca gcgattcaca acatcgcctt
    18001 tttcccagtc tttaggatcg tatttgcgac cattccattt cacaccatat tgtttcgcca
    18061 gtaatgcata cgtctggcga acgcgagatc cctcaattcc acgtaactgc tccactgaac
    18121 ggcgagctgg cggtggttca cgaaaacgta attcatacat tttgcgcacc accttcaacc
    18181 gtagatcttc cgttaaagcc agctttgcct agtagagtaa tttatctgcc cgcgcccctc
    18241 cgggttgtcc ggaggagtaa acgcgaacgc ccgcttcacc gacccagacc agcagtgttc
    18301 ccaccgtggc ggccagatgg actgccgcgt gggaaactct tgttcccggt tcgagcataa
    18361 tgcaggcgac cgatcccact ggaatatgcg tgcggatccc ggttttgtcg atcagcacga
    18421 aagcgccgtc cagtacgtcg atttgaccgt actggaggaa gatcatggaa gtgcgatctt
    18481 ttaatgggat cgggctcagc ggtacaaacg tcacgcctct gctccgggtt tgatcagcat
    18541 cagaccacaa ccgaacgccc ggcttttgcc atacccctga ctgagacgtt gcagaaataa
    18601 cccaggagcg gtgaccgtaa gcatccccgt ataatccacg ctactgaact ggatcagttg
    18661 ccgggagttt tcccgccgca gttgctgttg tcggtaggca tcaaccgaag tatccagcag
    18721 tgtaaaaccg cttctctccc cctgcgctgc cagccagtcc agcgcagcct gttgttgatg
    18781 caaccagaca tcacttcctt ccacctgccc cctcacctgc cgtttcgcct ccatcagcaa
    18841 gtcgtggcgt ttgcccgctt tacaaattgt tggattcgcc cgcaggttga aacaaaggga
    18901 ttgtccggta cgcaactctg gcgcaaatgg tcggcattcg atggtgaaaa tatcactttc
    18961 cgccgggcgt tcctgcgaca ggacaaaaaa gc
//
LOCUS       DAJTXQ010000081        18583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000081 DAJTXQ010000000
VERSION     DAJTXQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.081"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(238..2109)
                     /gene="ppiD"
                     /locus_tag="OO515_002672"
     CDS             complement(238..2109)
                     /gene="ppiD"
                     /locus_tag="OO515_002672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6576566.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2301..2573)
                     /gene="hupB"
                     /locus_tag="OO515_002673"
     CDS             complement(2301..2573)
                     /gene="hupB"
                     /locus_tag="OO515_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6576567.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2782..5136)
                     /gene="lon"
                     /locus_tag="OO515_002674"
     CDS             complement(2782..5136)
                     /gene="lon"
                     /locus_tag="OO515_002674"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6576568.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5324..6598)
                     /gene="clpX"
                     /locus_tag="OO515_002675"
     CDS             complement(5324..6598)
                     /gene="clpX"
                     /locus_tag="OO515_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6576569.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6724..7347)
                     /gene="clpP"
                     /locus_tag="OO515_002676"
     CDS             complement(6724..7347)
                     /gene="clpP"
                     /locus_tag="OO515_002676"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6576570.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7593..8891)
                     /gene="tig"
                     /locus_tag="OO515_002677"
     CDS             complement(7593..8891)
                     /gene="tig"
                     /locus_tag="OO515_002677"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6576571.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9235..9552)
                     /gene="bolA"
                     /locus_tag="OO515_002678"
     CDS             complement(9235..9552)
                     /gene="bolA"
                     /locus_tag="OO515_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6576572.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9857..10435
                     /locus_tag="OO515_002679"
     CDS             9857..10435
                     /locus_tag="OO515_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6576573.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10479..11954
                     /gene="ampG"
                     /locus_tag="OO515_002680"
     CDS             10479..11954
                     /gene="ampG"
                     /locus_tag="OO515_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6576574.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12415..13362
                     /gene="cyoA"
                     /locus_tag="OO515_002681"
     CDS             12415..13362
                     /gene="cyoA"
                     /locus_tag="OO515_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6576575.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13384..15375
                     /gene="cyoB"
                     /locus_tag="OO515_002682"
     CDS             13384..15375
                     /gene="cyoB"
                     /locus_tag="OO515_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6576576.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15365..15979
                     /locus_tag="OO515_002683"
     CDS             15365..15979
                     /locus_tag="OO515_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6576577.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15979..16308
                     /locus_tag="OO515_002684"
     CDS             15979..16308
                     /locus_tag="OO515_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6576578.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16320..17210
                     /gene="cyoE"
                     /locus_tag="OO515_002685"
     CDS             16320..17210
                     /gene="cyoE"
                     /locus_tag="OO515_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6576579.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17395..17706
                     /locus_tag="OO515_002686"
     CDS             17395..17706
                     /locus_tag="OO515_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6576580.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17690..18232
                     /locus_tag="OO515_002687"
     CDS             17690..18232
                     /locus_tag="OO515_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6576581.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18288..>18583)
                     /locus_tag="OO515_002688"
     CDS             complement(18288..>18583)
                     /locus_tag="OO515_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576582.1"
                     /translation="KLGEVANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYL
                     FGSFTSNSSLILYKPEFERKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT         4146 a         4886 c         4530 g         5021 t
ORIGIN      
        1 cacagaagct gccgccagcg cgctatgaga cattccggca caggccaggg acagggtaat
       61 gagcagtgct ttaattccgt gtttcatact gttttctcct tgtttgttaa cagtggaatc
      121 accttaacgg gagtgaaaac agtaaacaaa tggcaatgtt cagaaatgga aaaggccgcg
      181 aaagcgacct tttcttgtta cagctgcaac gcattttttt gcgaggcggc ttcaggatta
      241 ttgctgttcc agcgcatcgc caattttgat tttcgcctct ttacgcaggt tactcatcag
      301 agcttcaaag acgatttgtg cgttgttctg ggtgataccc tgcaccatcg cttttttctg
      361 atcttccggc attgaacctt gtttcacttc atccagcgcc agcagaacca cattaccttg
      421 catatcggtc gccataccgt agctcggttt gtctttcgct ggcagtggca gtgcaaacgc
      481 cgcctggcta atcgggtcac gaccggaacg gcttaaggtt ttcggctcgc caaatttcag
      541 accggcagcc tgcatagctt ccgccccttt gccggctttc aaatcaacca gcagtttctc
      601 agcatccact ttcgcctgtt gttcagcttt gttgtgctga accagtgcct taacttgttc
      661 ctgaacatct gccaacggtt tcaccgcttc cggtttgtgc tcgctgatgc gcagcacgaa
      721 tgcgcggtcg ccgtctacgg tgatgatgtc agagttgatg cccggcgcgc cgttttcacc
      781 taccagaccg ccgttaaaga tagcgtcggc aaccggcttg aagttcaact cttccggcag
      841 gttatctttg ctgaaccaac ccgtctgagt ggctttaacg ccggcagctt gctctgcacc
      901 ggccagagac tcggtgtcgt tgcttgccgc atcgctcact ttctgctgca gcgcatagta
      961 cgcatcgagg gctttttcgt gtttcacttt cgccgcaatg tcgtcacgta cttcgtctaa
     1021 cgatttcact ttcgctggct gaatgtcgtc cagacgtaca atcaggaaac cgaccgaaga
     1081 tttgatgaca ccagacagtt ggcctttttc tttcagacca gcatttttca gttcgtcggg
     1141 gatagtggca tcttctaacc aacccatatc gccgccgtta cgggcagaga taatatcggc
     1201 agatttttct ttggctaatg cagcaaaatc accgccttta ttcagctcat caagtaccgc
     1261 tttcgcttca tcttcagttt tggtctggat gatgctgtag cgggtacgct gcggctgggt
     1321 gaattgatcc tgatgctgat cgtagtagct ctggatatcc gcatcgctaa ccggttgctg
     1381 catcgttgcg gcatccagct tgatgtaact cacgcggaat tgttccggcg tcatgaaatt
     1441 gtttttgttt tgttcgtagt agctggcaat ctcctgttcg gtcacaggct gcttcgccgc
     1501 cagcgcgtta acatcgatag tcgcctcacg caccacgcgt tgttgcgcga ccagtgccgc
     1561 cagctcgtcg gtttcacctt tcagcataaa atcggtaccg gcaacaccgt taatcagctg
     1621 ttgggtagtg agctggttac gcagcgcctg ggcgtactga tcggcggtca tccccatctg
     1681 gttgaggata ccgttatagc ggctgttatc aaatttgccg tcaacctgga aggctggggt
     1741 cgcgaaaatc gcctgtttaa cctgctcatc actgataccc agtttcagct cacgagcgta
     1801 ctgatccaac agcgcctcgt cgatcagacg attcagcacc tgttgacgca gggttttcat
     1861 atagccttcg ttcgctgcca gctcggagta ttgatcgccc agctgttgct gcatgcgatt
     1921 acgctcgctg ttaaaggcgt tctcgaactg cccacggctg atttcctggt cattcacttt
     1981 tgcggcgtag ttattgcctc cgccaatcag gtaaccactc acgccggtca atatgaacga
     2041 cacgataatg ataccgaaaa taatcttgag cacgagactg tttgcagccg tgcgtaagct
     2101 gtccatcatg gtgtaacaac actccgctgt agatgactgt gtacctcacg caacgtatcg
     2161 atgcgcgcaa aggcctattg tgacaagaaa cgggggcaat tgtcagccca caagacacag
     2221 aaagtcaccg aatacaaata aaaaaggcac atcagtagat gcgcccttga acttcgtcac
     2281 atccccactg gggacaacgc ttagtttacc gcgtctttca gtgctttacc tgcacggaag
     2341 ctcggtactt tagcggcagc gatggtgatc tctttaccgg tctgcgggtt gcggccagta
     2401 cgggcagcac gctctttaac ggcaaaagta ccaaaaccta ccagtgctac atcatcccct
     2461 tctttcagag attcagttac ggaagcaata atagcatcta acgcacggcc agccgcagct
     2521 ttagagatat cagcccctgc agcaatcttg tcgatcaatt gagatttatt cactcttctc
     2581 ttcctcttta taatttatat cgcacttgaa tccttcaagg tacgaacgcg cagcagttat
     2641 atcaggccag ccatcccctt acaacacccc ttaataaggg caagcccgat ccgccatcta
     2701 acttagcgag acaaaaaaag gctggcaagc ccgaattagc ctgccagccc tgtttttatt
     2761 agtgcatttt gcgcgaggtc actattttgc agtcacaacc tgcatgccag acggttcatt
     2821 ttgcagcgcc agagtcagaa cttcctcaat gcgcttcaca ggatgaatgt ccagatcggc
     2881 aattacgttg tcaggaatct cttccagatc gcgtttattt tcgaacggaa ttagcactgt
     2941 tttaatcccg ccgcgatgcg ctgccaggag tttttctttc aaaccaccga tcggcagtac
     3001 ctgaccacgc agagtgatct caccggtcat tgccacatcg gcacgaaccg ggttaccggt
     3061 caggcaagaa accagcgcgg tgcacatagc aataccggca ctcggaccat ctttcggcgt
     3121 cgcaccttcc ggtacgtgga cgtggatgtc acgtttttcg taaaaatcag ggttgatccc
     3181 cagtttttcc gcacgcgcac gaaccaccgt taacgccgcc tgaatagact cctgcatcac
     3241 ttcgccgagc gaaccggtat aggtcagttt gcctttaccc ggaacacatg cggtttcaat
     3301 ggtcagcaag tcaccgccca cttccgtcca cgccagaccg gttacctgac cgacacggtt
     3361 ttcgttatca gcgcgaccat agtcgaaacg ctgaacaccg aggtagtcat gcaggttatc
     3421 gccgttaatt tcgatatgtt ttaatgactt atcgagcagt aactgcttaa ccgctttgcg
     3481 gcacagtttg gagatttcac gctccagacc acgcacgccc gcctcacggg tgtagtaacg
     3541 aataatgccg ataatggcgc tatcgtcgac ggtcagctca ccttttttca gtgcattacg
     3601 ttcaatctgc ttcggcagca ggtgacgttt ggcgatgttc agtttttcat cttcggtata
     3661 accggagagg cgaatcactt ccatacgatc cagcagcggt gccggaatgt tcatggagtt
     3721 cgacgtcgcg acaaacatca cgtcgctgag atcgtaatcc acttccaggt agtggtcgct
     3781 gaacgctacg ttctgctctg gatccagcac ttcaagcagt gcagaggccg gatcgcctcg
     3841 catgtcagaa gacattttgt cgatctcatc gagcaggaac agcgggtttt tcacgcccac
     3901 tttcgccatt ttctggatca gtttacccgg catagaaccg atgtaagtac ggcggtgacc
     3961 acggatttcc gcttcatcac gcacgccgcc cagcgccata cggacatatt tacgcccggt
     4021 ggctttggca atggactgac caagagaggt tttacctacc cccggcggcc ctaccaggca
     4081 gaggatcggt cccttgattt tgttgacacg gctttgaacc gcaaggtact caaggatgcg
     4141 atctttcacg cgctccagac cataatggtc ggtatcaagg atttcctgcg cctgacgcag
     4201 gtcttttttg accttgctgc gcgcattcca cggtacctgt accatccagt cgatataacc
     4261 acgcactacg gtcgcttctg ccgacatcgg agacatcatt ttcagcttct gcaactctgc
     4321 ttccgctttc tcttttgcct ctttcggcat cttcgccgcg tcgattttgc gcttcagggc
     4381 ttcgttttcg tccggcgcgt cgtccatttc accgagttct ttctgaatag ctttcatttg
     4441 ctcgttcaga tagtactcac gctgggattt ctccatctgc tttttaacgc ggttgcgaat
     4501 gcgtttctca acctgcagca gatcgatttc cgattccatc attgccatca gatattccag
     4561 acgttcgtta acgtcggaca tctccagaac ggactgtttg tcagccagtt tcagcggcat
     4621 atgtgcagca atggtatccg ccagacgcgc cggatcgtcg atgctattca gcgacgtcag
     4681 cacttctggt gggatttttt tgttcagctt gatgtagcct tcgaactggc tgattgcagt
     4741 acgcaccagc acttcctgtt cccgctcatc aatggtcggc gactccagat actccgcctt
     4801 cgcagaaaag tgttcgccat tgtcagagag cgcagaaata cgcgcgcgct gtaacccctc
     4861 gaccagcact ttgacggtgc cgtcaggcag tttcagcatc tgcaatatag aggccacggt
     4921 cccgacggtg aaaagatcgt ttacacccgg ctcatccgtt gaagcttctt tctgcgcgac
     4981 cagcataatt tttttatcat ggtccatcgc cgcttccaga caacggatag atttttcccg
     5041 cccgacaaat aaggggatga ccatgtgcgg ataaaccacc acatcgcgca gcggcaatac
     5101 ggggatttca atgcgttcag aacgctcagg attcatagag ctctctctta gtttaatttc
     5161 cgccaggtaa tcagatgaca cgactgtgct tcacgccatc tattaacatg tacgtcagta
     5221 tatggggatg tttcccccac attcaacgcc gagaatagag gaaaaattaa aggggagata
     5281 aaatcccccc tttttggtta actaattgta tgggaatggt taattattca ccagatgcct
     5341 gttgcgcttc cggcttacca taaatcagca acggtttgct ttgaccatca attaccgact
     5401 cgtcgataac cactttttcg acgtcttcca tggacggcag atcgtacatg gtatcgagca
     5461 gtgcggcttc tacgatggaa cgcaggccac gggcaccggt tttacgcgcc atcgctttct
     5521 tagcgatagc atccagcgcc tcgtcacgga attccagatc cacgccttcc agattaaaca
     5581 gcgcctgata ctgcttggtc agggcgtttt tcggctcttt gaggatctga atcagagctt
     5641 cttcgctcag ttcattcaac gttgctacaa ccggcagacg accaatgaac tcagggataa
     5701 gaccaaactt gatcagatct tccggttcaa cctgcgccag cagctcgcct tcgcttgctt
     5761 tgtcggactt cgcttttacc gtcgcgccaa aaccaatgcc ggagccggtt tctacacggt
     5821 gggaaatcac tttatccaga ccggcaaacg caccgccaca aataaacagg atcttagagg
     5881 tatcaacctg caagaattcc tgctgcggat gtttacgccc accttgcggt ggaacagcag
     5941 ctaccgtacc ttcgatcagt ttcaacagtg cctgctgtac gccttcaccg gaaacgtctc
     6001 gggtaatgga cgggttgtct gacttacgag aaatcttgtc gatttcatcg atgtagacaa
     6061 tcccgcgctg cgctttctgt acgtcgtaat cgcacttttg caacagcttc tgaatgatgt
     6121 tttcaacgtc ttcgcccaca taaccggctt cggtcagcgt ggttgcgtcg gccatggtga
     6181 acgggacgtc cagcaggcgc gccagcgttt cagccagcag cgttttaccg gaaccggtcg
     6241 gaccgatcag cagaatgtta cttttgccca actcgacacc attgctggta tcgccgttgc
     6301 gcagacgttt gtaatggttg tataccgcga ccgccagcac ttttttcgcc tgttcctggc
     6361 cgataacgta atcgtccagg tggttgcgaa tttcatgcgg cgtcggtagc gcactgcgtt
     6421 cacgatgcgg tgcaacttct ttaatctctt cgcgaatgat gtcgttacat aaatcaacac
     6481 attcgtcgca gatatacacg gatggaccgg caatcagctt gcgcacttca tgctggcttt
     6541 tgccgcaaaa agagcaatac agcaatttgc ctgagccatc tttgcgttta tctgtcatgg
     6601 gtcaaaacct cttctttgtt ctttgtgccg cacacgacga cgcaaatgcc attctcaggc
     6661 gcaagccgct tacagcgttg tgccgccctg gataagtata gcggcacagt tgcgcctctg
     6721 gcatcaatta cgatgggtca gaatcgaatc gaccagaccg tattccaccg cttcaggggc
     6781 ggaaaggaag cgatcgcgct cggtatcacg ttcaatctgt tctaatgatt gacccgtatg
     6841 aagcgccata agttcattca tgcgcccttt aactttcaga atttcacggg catgaatttc
     6901 gatatcggtc gcctggccct ggtagccgcc caacggttgg tgaatcatca cgcgcgaatt
     6961 cggcaggcaa aaacgtttac cttttgcccc tgcggtcagc aagaaagcgc ccatcgaggc
     7021 cgcctggccc atacagatgg tgctgacatc aggcttgata aactgcatgg tgtcatagat
     7081 agacatcccg gcagtgatca ccccgcctgg ggagttaatg tacagataga tatctttttc
     7141 tgggttttcc gcttctagga acagcatctg cgccacaatc aggttcgcca tgtggtcttc
     7201 aacctggcca gtcagaaaaa tgacgcgttc cttaagtaga cgagaataga tatcaaaaga
     7261 gcgctcaccg cgtgaggtct gttcaatgac catcggcacc agcgccatat ggggtgcaaa
     7321 gttatctcgt tcgccgctgt atgacatttc cgtctcctgg ataaaattga aaaaacctgc
     7381 tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat gggtacggca
     7441 tcaccatatt tcaagcataa caatcttttg ttattacgct aacaccgaaa gaggcttttc
     7501 gcacggttcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg tgacgggcct
     7561 ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat cagctcgttg
     7621 aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac agcctgttct
     7681 tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta gaactcgata
     7741 acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt cacgcgctct
     7801 tcgtcagctt tcagttcgtt ggtgcggata acttcgccca gcagcaggcc aacaactacg
     7861 cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg tttttcgttg
     7921 ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc gatcagcgca
     7981 gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga cttaacgcgg
     8041 ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc gcgcagacct
     8101 tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag ttccggcagt
     8161 tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc tttcagattt
     8221 tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc agctttgtgg
     8281 cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc cagtacgaaa
     8341 tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa gtcgatggtt
     8401 acgcggtctt ctgcttcaac ggcgccgtct ttttctttcc aggtcgcctg ctgtttacgc
     8461 agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg tttttcaact
     8521 tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc tacagagtag
     8581 gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc agccggattg
     8641 attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc cagaacgtcc
     8701 tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt gcctttgcgg
     8761 aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac agcggtctcg
     8821 atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt ggtttcaact
     8881 gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt accccgcaag
     8941 cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat tccctgttac
     9001 cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc acttttatga
     9061 gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg caattttcgt
     9121 ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc aggccgtttt
     9181 tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc gtttttacgc
     9241 gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca acccttccca
     9301 ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga gttcctccgc
     9361 taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa aacgatcgct
     9421 gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac gatagctttc
     9481 atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt ctatccgctc
     9541 acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc cctttaaata
     9601 ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt tcgtcctggt
     9661 ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc ctcgcatcag
     9721 cgtttctttt accaggggtg attatttcgc caacatgatg aatttacatg ccttacccac
     9781 ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac gcttaagagt
     9841 ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc tgtttatgct
     9901 tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta cactgccaca
     9961 gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc agcgtaccga
    10021 tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg cctcccgaga
    10081 tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg gttacatggt
    10141 tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg ctgacgtgtc
    10201 ccagggcaac gtgcgctaca acatcgcgac caaagcggat atcgccatca tcgctaccgc
    10261 gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg aaggtgcgtt
    10321 ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg ataccatcgc
    10381 tgatatgtct caggacacca gcatccacga attcatcaag cagaacgcgc gttaatttct
    10441 gccctctggc ccggtgcaaa ccgggcctgt agacgcccat gtccagtcaa tatttacgta
    10501 tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc gggctaccgc
    10561 tcgccctgac atccggtacc ttacaggcct ggatgacggt cgagaatatt gatctcaaaa
    10621 ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc tggtcaccgc
    10681 tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg ctcgccacgc
    10741 aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaacctggc acccaactcc
    10801 gctggatggc ggcactggcg gtggtgatcg ctttttgctc ggcctcccag gatattgtct
    10861 tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc gcggcaatca
    10921 gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cttagccctg tggctggcag
    10981 ataaatggct gggctggcag ggcatgtact ggttgatggc ggcgctgttg atcccttgta
    11041 ttatcgcgac gttgcttgca ccagaaccga ccgacaccat tcctgtgcca aaaacgctgg
    11101 aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc tggcttattt
    11161 tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg acaaccacgt
    11221 ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggttaac aaaacgcttg
    11281 gcttactggc gaccattgtg ggcgcattgt acggtgggat tttgatgcag cgcctgtcgc
    11341 tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct ggttactggc
    11401 tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt ttcgaaaacc
    11461 tctgtggcgg gatgggcaca tcagcctttg tcgcactgtt aatgacgctg tgtaataagt
    11521 cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtagga cgagtttatg
    11581 tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc tatctattct
    11641 ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag acgcttgaat
    11701 atacacgagt aaatgacaac tttatctccc gtaccgaata tccgacaggt tatgcctttg
    11761 ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactg ctgttgacga
    11821 tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa gtcggggttt
    11881 tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggct ctacgaaaaa
    11941 cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc gtaattatta
    12001 cggctaaata aacatcagta tcgcttaata atatttgttc ttttgtgcgg cttagcgttt
    12061 ggaaattatt gacgcaattt atgaattcta ttttttctta cacgattcag ctaatgagtc
    12121 tttattttcc ccccacccaa ttgtcaccat aatgataatt atttgttaaa caattgtttt
    12181 attcaagatt ggttatacca attgcccgtc caaactcctt ctgcactccc ctttcgttat
    12241 aacgcccttt tgcaacagct tcttaaaatc aacttgatat gttttgcaac atatgtaacc
    12301 tgacagccaa atccaagtaa caggaattta atcatgttta cagtaatgta acctccccgt
    12361 aaaatgccca cacactttaa acgccaccag atcccgtgga attgaggtcg ttaaatgaga
    12421 ctcaggaaat acaataaaag tttgggatgg ttgtcattat ttgcaggcac tgtattgctc
    12481 agtggctgta attctgcgct gttagatccc aaaggacaga ttggtctgga gcaacgttca
    12541 ctgatactga cggcatttgg cctgatgttg attgtcgtta ttcccgcaat cttgatggct
    12601 gttggtttcg cctggaagta tcgtgcgagc aataaagatg ctaagtacag cccgaactgg
    12661 tcacactcca ataaagtgga agctgtggtc tggacggtac ctatcttaat catcatcttc
    12721 cttgcggtac tgacctggaa aaccactcac gctcttgagc ctagcaagcc gctggcacac
    12781 gacgagaagc ccattaccat cgaagtggtt tccatggact ggaaatggtt cttcatctac
    12841 ccggaacagg gcattgcaac cgtgaatgaa atcgctttcc cggcgaacac tccggtgtac
    12901 ttcaaagtga cctccaactc cgtgatgaac tccttcttca ttccgcgtct gggtagccag
    12961 atttatgcca tggccggtat gcagactcgc ctgcatctga tcgccaacga acccggtact
    13021 tatgacggta tctccgccag ctacagcggg ccgggcttct caggcatgaa gttcaaagct
    13081 attgcaacac cggatcgcgc cgaattcgac caatgggtcg caaaagcgaa acagtcgccg
    13141 aacaccatgt ctgacatggc tgcgttcgaa aaactggccg cgcctagcga atacaaccag
    13201 gtggaatatt tctccaacgt gaaaccagac ttgtttgccg atgtgattaa caagtttatg
    13261 gctcacggta agagcatgga catgacccag ccagaaggtg agcacagtgc acacgaaggt
    13321 atggaaggca tggacatgag ccacgcggaa tccgcccatt aaaggggttg aggaagaata
    13381 aagatgttcg gaaaattatc acttgatgca gtcccgttcc atgaacctat cgtcatggtt
    13441 acgatcgctg gcattatttt gggaggtctg gcgctcgttg gcctgatcac ttacttcggt
    13501 aagtggacct acctgtggaa agagtggctg acctccgtcg accataaacg cctcggtatc
    13561 atgtatatca tcgtggcgat tgtgatgttg ctgcgtggtt ttgctgacgc cattatgatg
    13621 cgtagccagc aggctcttgc ctcggcgggc gaagcgggct tcctgccacc tcaccactac
    13681 gatcagatct tcaccgcgca cggcgtgatt atgatcttct tcgtggcgat gcctttcgtt
    13741 atcggtctga tgaacctggt ggttccgctg cagatcggcg cgcgtgacgt tgcgttcccg
    13801 ttcctcaaca acttaagctt ctggtttact gttgttggtg tgattctggt taacgtttct
    13861 ctcggcgtgg gcgaatttgc gcagaccggc tggctggcct atccaccgct atcgggaata
    13921 gagtacagtc cgggagtcgg tgtcgattac tggatatgga gtctccagct atccggtata
    13981 ggtacgacgc ttaccggtat caacttcttc gttaccattc tgaagatgcg cgcaccgggc
    14041 atgaccatgt tcaagatgcc agtatttacc tgggcatcac tgtgcgcgaa cgtactgatt
    14101 attgcttcct tcccaattct gacggttacc gtcgcgttgt tgaccctgga tcgctatctg
    14161 ggcacccatt tctttaccaa cgatatgggt ggcaacatga tgatgtacat caacctgatt
    14221 tgggcctggg gccacccgga agtttacatc ctgatcctgc ctgttttcgg tgtgttctcc
    14281 gaaattgcgg caaccttctc gcgtaaacgt ctgtttggtt atacctcgct ggtatgggca
    14341 accgtctgta tcaccgtgct gtcgttcatc gtttggctgc accacttctt tacgatgggt
    14401 gcgggcgcga acgtaaacgc cttctttggt atcaccacaa tgattatcgc catcccgacc
    14461 ggggtgaaga tcttcaactg gctgttcacc atgtatcagg gccgcatcgt gttccattct
    14521 gcgatgctgt ggaccatcgg ttttatcgtc actttctcgg tgggcgggat gactggcgtg
    14581 ctgctggcag taccgggcgc ggacttcgtt ctgcataaca gcctgttcct gattgcgcac
    14641 ttccataacg ttatcatcgg cggcgtggtc ttcggctgct tcgcagggat gacctactgg
    14701 tggcctaaag cgttcggttt caaactgaat gaaacctggg gtaaacgcgc gttctggttc
    14761 tggatcatcg gcttcttcgt tgcctttatg ccgctgtatg cgctgggctt catgggcatg
    14821 acccgtcgtt tgagccagca gattgacccg cagttccaca ccatgctgat gattgcagct
    14881 agcggtgcag tactgattgc gctgggtatt ctctgcctcg ttattcagat gtacgtttct
    14941 attcgcgacc gcgaccagaa ccgtgacctg actggcgacc cgtggggtgg ccgtacgctg
    15001 gagtgggcaa cctcttcccc gcctccgttc tataactttg ccgttgtgcc gcacgttcac
    15061 gagcgtgatg cattctggga aatgaaagag aaaggcgaag cgtataaaaa gcctgaccac
    15121 tatgaagaaa ttcatatgcc gaaaaacagc ggtgcaggta tcgtcattgc agctttctcc
    15181 accatcttcg gtttcgccat gatctggcat atctggtggc tggcgattgt tggcttcgca
    15241 ggcatgatca tcacctggat cgtgaaaagc ttcgacgagg acgtggatta ctacgtgccg
    15301 gtggcagaaa tcgaaaaact ggaaaaccag catttcgatg agattactaa ggcagggctg
    15361 aaaaatggca actgatactt tgacgcacgc gactgcccac gcgcacgaac acgggcacca
    15421 cgatgcaggc ggaaccaaaa tcttcggatt ttggatctac ctgatgagcg actgcattct
    15481 gttctcaatc ttgtttgcta cctatgccgt tctggtgaac ggcaccgcag gcggcccgac
    15541 aggtaaggac attttcgaac tgccgttcgt tctggttgaa actttcttgc tgttgttcag
    15601 ctccatcacc tacggcatgg cggctatcgc catgtacaaa aacaacaaaa gccaggttat
    15661 ctcctggctg gcgttgacct ggttgtttgg tgccggattt atcgggatgg aaatctacga
    15721 attccatcac ctgattgtta acggcatggg tccggatcgc agcggcttcc tgtcagcgtt
    15781 ctttgcgctg gtcggcacgc acggtctgca cgtcacttcc ggtcttatct ggatggcggt
    15841 gctgatggta caaatcgctc gtcgcggcct gaccagcact aaccgtaccc gcatcatgtg
    15901 cctgagcctg ttctggcact tcctggatgt ggtttggatc tgtgtgttca ctgttgttta
    15961 tctgatgggg gcgatgtaat gagtcattct accgatcaca gcggcgcgtc ccatggcagc
    16021 gtaaaaacct acatgacagg ctttatcctg tcgatcattc tgacggtgat tccgttctgg
    16081 atggtgatga caggagctgc ctctccggcc gtaattctgg gaacaatcct ggcaatggca
    16141 gtggtacagg ttctggtgca tctggtgtgc ttcctgcaca tgaataccaa atcagatgaa
    16201 ggctggaaca tgacggcgtt tgtcttcacc gtgctaatca tcgctatcct ggttgtaggc
    16261 tccatctgga ttatgtggaa cctcaactac aacatgatga tgcactaaga gcggcggtta
    16321 tgatgtttaa gcaatacctg caagtaacga aaccaggcat catctttggc aacctgatct
    16381 cggtgattgg gggattcctg ctggcctcaa agggcagcat tgattatccc ctgtttatct
    16441 acacgctggt tggggtgtca ctggttgtgg cgtcgggttg tgtgtttaac aactacatcg
    16501 acagggatat cgacagaaag atggaaagga cgaagaatcg ggtgctggtt aaaggcctga
    16561 tctctcctgc tgtctcgctg gtgtacgcca ccttgctagg ctttgctggc tttatgctgc
    16621 tgtggtttgg cgcgaatccg ctggcctgct ggctgggggt gatgggcttt gtggtttatg
    16681 tcggcgttta tagcctgtac atgaaacgcc actcggtcta cggcacattg attggttcgc
    16741 tctccggcgc tgcgccgccg gtgattggct actgtgcagt aaccggcgag ttcgatagcg
    16801 gcgcagcgat cctgctggct atcttcagcc tgtggcagat gcctcactcc tatgccatcg
    16861 ccattttccg ctttaaggat tatcaggcgg caaacattcc ggtattgcca gtggtaaaag
    16921 gcatttcggt ggcgaagaat cacatcacgc tgtatatcat cgcctttgcc gttgccacgc
    16981 tgatgctctc tcttggcggt tacgctgggt ataaatatct ggtggtcgcc gcggcggtga
    17041 gcgtctggtg gttaggtatg gccctgcgcg gttataaagt tgctgatgac aggatctggg
    17101 cgcgcaagct gttcggcttc tctatcatcg ccatcacagc cctctcggtg atgatgtccg
    17161 ttgattttat ggtgccggac tcgcatacgc tgctggctgc tgtttggtaa cacaacctct
    17221 ctattaaaaa ggtgctacgg cacctttttt attgtctgag atcagagata cggctaataa
    17281 ccgtatgtct tgacagagtg agcagatgaa cataaattaa tcatacggtt gatagccgta
    17341 ttatgtatga gatcgactcc ctacgatttc attcagttaa tcaaagaggt cataatgcct
    17401 ggaaaaacag ccactctacc cgatgtcgat aaaacgctga aaaatgcgcg ggtcgaactc
    17461 aaaactagtc ccgacgccaa aaacaaactt cgtgaagctg cgcaagccgt tggggtagat
    17521 ttaagcgctt ttattttgag cgctgctatg gaacgcgccg aaagtgtgct tgataaccaa
    17581 cgtcgccgtg agctttcgaa tcaaagctgg gaactgatga accaactcat cgctgaacct
    17641 gctcaaccga cgctcgccct caaggcgtta atgaaaagga aaaacagcga tggtcgacaa
    17701 gcatgaagag attactctgc ccatagtcct ctcctgtaat tatcagtctg atattactta
    17761 tcctgggcaa aaacagtttg attgcggtaa ccctgttatc gataaatttg tacgcgcatc
    17821 gctaaagaaa agtgtgcgta atagcgactg tgcggctaaa gcacttattg acagacaaag
    17881 tggtgaactg atcggcatct gtacttttac ggcatattcg ctggaaaaac aacgcgtttc
    17941 tggcgtcctt cagggttcac aaccttcaga aattggtgtt gtcagattag tcatgttggg
    18001 ggtagcacgg aagtatcaaa agcggggctt tggtcaggac ctactatgtg atttttttga
    18061 acatgtaaaa ataattcacc aggcattacc aattaaaggg gtttatcttg atgctgaccc
    18121 tgccgccatt aatttttatg ctcgtctcgg ctttgttcag ctttcagcga caccaaatgc
    18181 ttttggtgct gtacctatgt ttttggcgat tcagcatatt ctcgcggctt agattattat
    18241 cgccacccgc cagggcaagt tttgccttag ccgggggctg agcagcgtta ccacttttgt
    18301 tcaattgccc ccgctttcag caactccaca tccttgccgt taatggttgc cgttgcggca
    18361 acgattgtgg tccttacagt tgcacaagat tttcgctcaa attccggttt ataaagaata
    18421 agagacgaat tactggtaaa tgaaccaaat aggtaatcag taaagctaat attcagccgg
    18481 tttaaaccga aaggcgcgaa gccaaccgtt tggttaatct ccttaccgga tgggcgacga
    18541 atgctgaatg tcacgcgaag attggccact tcgcctaatt tat
//
LOCUS       DAJTXQ010000082        18524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000082 DAJTXQ010000000
VERSION     DAJTXQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.082"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1711
                     /locus_tag="OO515_002689"
     CDS             <1..1711
                     /locus_tag="OO515_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6576583.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1712..3361
                     /locus_tag="OO515_002690"
     CDS             1712..3361
                     /locus_tag="OO515_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6576584.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3366..4142
                     /locus_tag="OO515_002691"
     CDS             3366..4142
                     /locus_tag="OO515_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6576585.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4216..5400)
                     /gene="atoB"
                     /locus_tag="OO515_002692"
     CDS             complement(4216..5400)
                     /gene="atoB"
                     /locus_tag="OO515_002692"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCR6576586.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5431..6753)
                     /locus_tag="OO515_002693"
     CDS             complement(5431..6753)
                     /locus_tag="OO515_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCR6576587.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6750..7400)
                     /gene="atoA"
                     /locus_tag="OO515_002694"
     CDS             complement(6750..7400)
                     /gene="atoA"
                     /locus_tag="OO515_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCR6576588.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7400..8062)
                     /gene="atoD"
                     /locus_tag="OO515_002695"
     CDS             complement(7400..8062)
                     /gene="atoD"
                     /locus_tag="OO515_002695"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCR6576589.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8258..9643)
                     /gene="atoC"
                     /locus_tag="OO515_002696"
     CDS             complement(8258..9643)
                     /gene="atoC"
                     /locus_tag="OO515_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCR6576590.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9640..11466)
                     /gene="atoS"
                     /locus_tag="OO515_002697"
     CDS             complement(9640..11466)
                     /gene="atoS"
                     /locus_tag="OO515_002697"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCR6576591.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11633..14482
                     /gene="rcsC"
                     /locus_tag="OO515_002698"
     CDS             11633..14482
                     /gene="rcsC"
                     /locus_tag="OO515_002698"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6576592.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14682..15332)
                     /gene="rcsB"
                     /locus_tag="OO515_002699"
     CDS             complement(14682..15332)
                     /gene="rcsB"
                     /locus_tag="OO515_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6576593.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15349..18021)
                     /gene="rcsD"
                     /locus_tag="OO515_002700"
     CDS             complement(15349..18021)
                     /gene="rcsD"
                     /locus_tag="OO515_002700"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6576594.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4536 c         4867 g         4595 t
ORIGIN      
        1 tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc tggcgcagaa
       61 cattcagcaa tctggcttgt taagcgcaga actgcaacaa aatgggcaag tgcaggacag
      121 cattagcaca aaactgtctt ttgtggataa cagctggccc gttgaacagc agaaaaatgt
      181 catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggctagca atatccggct
      241 acaaagtagc gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt tagtcgatgg
      301 accatggggt ggcgtgatca acaccggtag ccgcctgatc ccgctcagtc tcgcctggcg
      361 ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga ttcaggataa
      421 ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt ggggtgaaga
      481 tggcaatggt gacgccttcc ttacggcatg ggcatggtac gccgactggc aggccagcca
      541 ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca gttatgccga
      601 gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg cacaggagat
      661 gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc ggcgcggaac
      721 taaagatgaa gatttctctg aggaagacat ccgcgatatc aatgacagcc tgatcctcga
      781 tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga cgttgctgaa
      841 aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt gggataaagc
      901 ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg gtggtgacgc
      961 tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta ttgagcgcgc
     1021 gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg ttttgcctgc
     1081 gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaagact ggcgttgggt
     1141 tggtcagggt gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc aaaatgtgca
     1201 ggtccgctgg cgtgaaccgg caaaaacggc tcaacaaagt aacattccgg tgaccgttga
     1261 acgccagttg tatcggctta tccccggtga agaagagatg agctttactc tgcagccggt
     1321 gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaattacgc ttaccagcga
     1381 gcaggatgca gttctgcgct atggtcaggt ggaagtaccg ctcccgccgg gagccgacgt
     1441 tgagcgcaca acatggggca tttcagtcaa taaacccaac gccgcgaaac agcaggggca
     1501 attgctggaa aaagcgcgta atgaaatggg cgaactggcc tatatggtgc cggtgaaaga
     1561 actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag ggcaattcgt
     1621 tctgcctcct gctcgttatg tgcgttccta tgcacctgcg cagcaaagtg ttgcggcagg
     1681 gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg tctggctatt
     1741 ggcgttggtt actctgccaa cgcttgcgga ggagacgccg ttacaactgg tgctgcgcgg
     1801 tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccaaag tctcagcatt
     1861 acctgactcg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt acgcctggct
     1921 ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc cggaagaggt
     1981 ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac gttcctgtgg
     2041 gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc aatactggca
     2101 acagcgtcag gcacctgcgt ggttagcgtc tcttacaacg ctgaagccag aaacctcggt
     2161 aacggttaaa tcgctgctcg attcattagc gacgttacct gcgcaaaaca aagctcagga
     2221 agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca tgctcggtag
     2281 tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa tccgccaggg
     2341 tggttttgct ggctggttaa ccgatggtac accgctgtgg gttactggca gtggcaccag
     2401 caaaacggta ctaacccgtt atgcgacggt gcttaatcgg gttttgccag tgccgacgca
     2461 ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc tgaagaaaat
     2521 cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc gctatcgtgt
     2581 gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga ccttactgag
     2641 cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg ttgccagagt
     2701 tctggatcgc gaggcgaaaa gtacgccacc tgaggcagcg aaagccatga ccgtggcgat
     2761 acgtactttt ttacagcaaa acgccaatcg ggaaggtgat tgcctgacca tccccgacag
     2821 cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa cgatgaccgc
     2881 ttggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca gtcgcgctac
     2941 cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcaggggg agcgttacga
     3001 ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag ctccacgctc
     3061 aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc cgcagtggcg
     3121 gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg tctgtcgttt
     3181 agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc gtaacttctt
     3241 tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct ttgatggtta
     3301 tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat taatggacta
     3361 atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct gtcgctcaca
     3421 gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat ccggcagagg
     3481 gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc aacatggcag
     3541 atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct gcggcgggta
     3601 aagttcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga attgaatccg
     3661 atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg caggtcatca
     3721 gcccggatgg gcaaagccgg caaaaaatgc agttttactc aacgtcgggt ataggaacga
     3781 ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat ctcgatctcc
     3841 atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg aaaaacagtg
     3901 gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc atgccagcac
     3961 cagttcacgg gcgctatcag gtgtatatca actactatgg tgggcgcagc gaaacggaat
     4021 tgacgaccgc ccaactgacg ctgatcaccg atgaaggatc ggtcaatgag aaacaggaaa
     4081 catttattgt accgatgcgt aatgctggcg agctgacgct ggtgaaaagt tttgactggt
     4141 gattaatggg gttaccttcg ccgtcgatgt tcaagaaaac gcccgataac tttcgctatc
     4201 gggtgttttt attgattaat tcaaccgttc aatcaccatc gcaattccct ggccgccacc
     4261 aatgcacagt gttgccagcc ccagcgtttt atcgcgtgcc tgcatggcat gtaatagtgt
     4321 gaccagaata cgagcaccac tggcaccgat aggatgcccg agcgcgatgg ccccgccgtt
     4381 gacattcact ttctcagaat caaagcccag ggttttccca acggcgagga actgtgcagc
     4441 aaatgcttca ttagcttcga tgagatcaat gtctgccagt tgcagccccg ccagttgtaa
     4501 cgctttttgc gtggcaggta ctggccccat acccatcaat gcgggaggca cgccaccgct
     4561 ggcataactt ttaatgcgag ccaggggggt aaggcctgct gccagcgccg cagattcttc
     4621 cataatcacc agagcggcag caccgtcgtt aataccagac gcgttcccag cggtgactgt
     4681 tcctgcttta tcgaaggccg ggcgcaatgc acctaacgct tcagccgttg aattcgcttt
     4741 cgggaattcg tcttgactga agacgaaggt tttcttccga gtgacaacat ttaccgggac
     4801 gatttcggct gtaaaagcac cggactcaat tgcggctgcc gctttacgct gtgaatgtag
     4861 cgccagttca tcctgcattt cacgggtaat tccgtactct ttagccacgt tttcggcggt
     4921 aatccccata tgataaccat gggtggcgca catcaggcca tcgcgcagga ttacgtcata
     4981 aacctgtccg tctccaagac gataaccaga gcgtgctttt gcatcgagta agtagggcgc
     5041 taaactcata ttttccatac cccccgccac aatgctcttc gcctgacctg cctgaattgc
     5101 ctgggcggca agcgccacac ttttaagacc cgaaccacac actttattga ccgtgaatcc
     5161 gcacaccgtt tctgccagcc cgctttttaa cagtgcctga cgtgccggat tttgccccaa
     5221 cccggcttgc aacacgttac ccataatcac ttcatcaatg tgtagtgaat cgatttttgc
     5281 acgttcaatg gcggctttaa ttactgtcgc ccccaggtcg atggcgctgg tggaagcgag
     5341 tgaaccgtta aaactaccga tagcagtacg taccgcactg acgatgacac aatttttcat
     5401 tttatattcc ttctgtctgt agggttgccg tcagaacagc gttaaaccaa tgacgaaaat
     5461 gacgccggag aagagcaggg cagtgatgca atagcccatg atgtcgcgga caccgagtcc
     5521 ggcgattgcc agtgctggca gcgcccagaa tggttgttcc atgttcatcc attgctcgcc
     5581 gtaggcgatc gccattaccg atttaccgag atcagcgccc agcgcctggg ctgcgggtat
     5641 cacgaaaggt ccctgaataa cccagtgacc gccgccagac ggaacggcga agttaatcag
     5701 tgcagaacta aaaaaggtca ttaccgggaa ggtgtctttg ttcgcaacat tgatgaagaa
     5761 ttcggtaatg agtccaccca gaccagaatg ctccatcatc agttggatcc cagcgtagaa
     5821 ggggaattgc accagaatac cggcagtact gcgtgctgcc gcgctgatag cacgcatata
     5881 agccattggc gttttatgta gcaacagacc cgcaatcata aacatcaggt tgacggtatt
     5941 gatggtgatg ttgaagccat gttcgctgaa gtacatcgcc aggtaagcga taccgagtgc
     6001 gccgatgatc aacgtcagaa tgcggctttc ttccagtcgc tcggatggtg gggcatcttt
     6061 cggtagctgc ttttgaaaat cagcctcttc catgagtagt tccggatcga tactcaccac
     6121 gtcagacggt tttggcatca tcatgcgggt gataaatggc atcaccacaa tcaacgtcac
     6181 agtgatgaaa atgttaaaac cactgaacag agtatcgccc accgggatca gcccggcgat
     6241 atgctcaacc gggttgcccg gtgttgcagc caacagaggc attgatccag agaagccgcc
     6301 accccaggtg agaaaaccaa tgtaggcgca ggcaatgagc aacggataat cagaaccggg
     6361 gactcgccgg gcgacttcac gggcaaacat tgcgccgaca accaaaccaa atccccagtt
     6421 gatgacacaa gcgactgaac cgaagaaagt gaccagcatg acgccctgta cgggcgtctt
     6481 tgcggcggag gcggcagtac gcagcaaact tttcaccgga gcagagctgg caagggcatg
     6541 accggtaacg atgataagcg ccatctgcat accaaacgcc agcaagttcc agaaaccgtc
     6601 accccacatt ttcaccatgc tgatcggcgt ttgtggtgtt aaccaaagcg cgatcacgaa
     6661 tgttagcaat gtcagcaaca tggcaaagat cagtggatcg ggaagccatc ggctgacaaa
     6721 acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt attcagatcg
     6781 gcggcgactt caaaccgagc ttctgttttg gcacgcacgg tggctaaatc acacccgtcg
     6841 gcaatttcgg tgagccacat tttgccgtca ataaaacgaa agacagccag ttcagtaacc
     6901 agcatatgca ccgcatgttg cgcagtgagt ggcatggtgc agctgcgcaa aatttttgct
     6961 gaaccatctt tggcgcaatg ttccatggcg atgatcactt tgcgcgaccc ggtcaccaga
     7021 tccatcgcgc cacccatacc gggcaccatt ttcccaggca ctacccagtt cgcgaggttt
     7081 gcttcttcgt ctacttgcaa accgccgagc acgcaggcat caatatgacc gccacggatt
     7141 agcgcaaatg acatggcgct atcaaacatg gctgcaccgg gtaaaacacc gcacggttgc
     7201 ccgccagcgt tcaccagatc tggatgcgct gtcgtgaccg ggcctaaacc gaggaagccg
     7261 ttttccgatt gcagagtgat atgaataccc tccggtaaat aattggcgac cattgtgggt
     7321 aaaccgatcc ctaagttaac gatgtcacca tcacgaagct cttgcgccac acggcgcgca
     7381 atacgttgtt tcgcatccat tatttgctct cctgtgaaac gatgatgtgg tcgataacgg
     7441 caccaggggt gacaatatgg tcaggttgca gctcgccggt ttcgaccagt tcatctggct
     7501 ctaccagcgt gatatcagcc gcaagggcta tcagggggtt aaagttgcgg gcgctaagtt
     7561 gataggtcag gttgccaagt gtgtcgcaac gatgagcgcg aattagcgcc aggtcggcgc
     7621 gcagtgggcg ttcgagcagc caggttttac cgtcgagtgt cagtgtctgt ttgccttcct
     7681 ctacgacggt gccgacaccc gttggggtga gaaaaccacc aagtccagct ccaccacagc
     7741 gaatttgctc gattagcgta ccttgcggca ccagaacgac gtccatctca ccagatatca
     7801 tgcgccgacc tgtttccggg ttggtgccga tatgtgaagc aatcactttg cggactcgac
     7861 cattgacgat gagcggaccg atgccggtat caacaaacgc ggtatcattg gctatcaatg
     7921 tcaggtcgcg aacaccagat tccagtaatg cttcaaccag gcgggatgga gtgccaatcc
     7981 ccataaatcc gcccaccatg atggtcatgc cgtcacgaaa gaagccggtg gcgtcttgta
     8041 atgtcatcaa ttttgttttc attttttatc cctcttgcat acaccgttgt gggtacagtc
     8101 aggcaatagc aaggggagtg ccagaatgag attattggat ttagttattt aactaattga
     8161 atatgaaggg ataaattgga tggggattta taaggagaat gagcggctat gcagaaaatt
     8221 gcgcactgtg caaatttctg catagcaagt tttggtgtta tacatccgcc ggatcgatac
     8281 cgtattcctg gagtttatac atcaatgcac ggcgactgat gcccagcatt aaagcagtgc
     8341 gggttcggtt tccttcttgt tgttccagca cttccataat gatgcgtttt tcgacgcgtt
     8401 taatttcctc ttttaaatta cgctcaccga cagaggctgt ttttacctcg ccagcattac
     8461 agactggctg acgaatctgt ggcggaagat cctcagaaaa aatgatcggg cctgaattca
     8521 tcacgacggc gcgttcaata acgttagaaa gctctcgaat atttcccggc catgaccagg
     8581 cggtaagcag tgacattgcc atcggatcga tgtcgataat atcgcgctga ttctcactac
     8641 tgaatttttg caaaaagtga ttagctaaca gggaaatatc ttcccggcga tcgcgcagag
     8701 gcggcagtat taaatgaata acgttaaggc gataaaagag atcttcacgg aaggtgcctt
     8761 cttttaccat tgcctgcaag tcacggttgg tggcagcaat gatgcggata tcaactttta
     8821 tggtctgatg gccgccaatc cgttcaaatt cccgttcctg tagaatgcgt agtaatttgg
     8881 cttgtagtac cagcggcatt tcgccaattt cgtcgaggag cagagtacct tcgttggctc
     8941 gttcaaataa tccctgacgc aaggtttgtg caccagtaaa tgcacctttt tcatgaccaa
     9001 acagttcact ttcgagcaac gattccggca gcgccgcgca gttgacttta atgaacggcc
     9061 cctttgcccg ccgcgaattg tagtgaatcg ctctggcaat caactctttc ccggtgccgc
     9121 tttcaccgct aatcaagacg ctggcctgag aaagggcaat tttggcggtg tctttgcaga
     9181 tgtccatcat cgccgggctg ttggtgagaa tgtgccccca ttgccagctg gcgctcagtg
     9241 cctggtgcag atgacggatc tcttttttca ttgactggag ttgtaaagcg cgctgaacga
     9301 ttaaattcaa ctcatcgaga tcaaacggtt taataacata gtcgaaggct ccgcagcgta
     9361 gcgcttcgac ggcggtttcc acttccgcat aggccgtcat cagaataacg ggtgtccggg
     9421 tctcatggct gcgcatctcc tttagtgcct tgatgccgtc catctctggc atgcggatat
     9481 ccatcaacac cacatcaggg tgaatatcgg caaacaggtg taatgctgtg cgtccgttgt
     9541 tcgcacaatg tgtttcgaat ccttgtagtg caaaagcggt gctcagcata cggcgaacat
     9601 tatcttcatc atccacaata aggatgcgat taatagcagt catacagtct gatttccctg
     9661 cgggttgatc ggtaaaataa gcgtgaaggt tgcgccgtag cccggcaaac tggcgacgcg
     9721 aatatcaccc tgatgggcat taatgatgcg ttgacttaac gccagaccaa gcccggttcc
     9781 tgaggctttg gtggtgaaaa agggatcgaa gatctttttt tgcagcgaga gatcaatgcc
     9841 acagccgttg tcctctatcg aaatggcctg ttgtgagtcg ctgtattgcc aggtttgaat
     9901 gcgaattttc cctcgtgcgc tgatagcctg gacggcattg atcaggatat tcagtagtac
     9961 ctgtttgagc agttcacgat cggcgttaat cgggcttaat tcattatcca gttcgcttat
    10021 gaagtcgacc cgcgcttgta cgccggcggt ttgtaccagt accagagttt cttcaaccaa
    10081 tgcattgagg ctgacttgtt gccattgact gtggcgtgga cgtgaaaatt cgagcaattg
    10141 ctgaataact ttgttaattg aatcgatttc tttgagtact acggacagat attcctgatg
    10201 tattgggtca ctggtttgtt ggcgcaagat ctgtacataa ccacgaatag ccgttaacgg
    10261 attacgtact tcatgcgcga cgccagccat cagctcaccc agtgtggcga ggcgttctgc
    10321 ttgcgccatg cggcgctggg tttctttgcg ggcagttaaa tcagagaaaa tcaccaaagc
    10381 acctatcatt tcaccgtgcg tgttatgaat acgactggta gtgacactga gttcaatggt
    10441 gcggtcacga cctggaaaac tgatctccag cgccacatgt tcggtgccat gttccagcgt
    10501 atccagtact ggactgtaga actgagtatt gtcgaacaac atggagtaag gctgccctac
    10561 cagttcatgg cgttgatagc cagtgataac ttctgctgct gggttcatgg tggttacatc
    10621 accctggcgg tcaatggcaa tgacgccatc ggcagcgttt tcaataatca gatcgttaag
    10681 tgtccgcgtt tcacgcagtg cctgggcgag gttattaaca ctctgactga tttgccccat
    10741 ttcaccgggc aattgtggta atcgagtggg aatattttgt gccagagtcg agaggccatc
    10801 ggtgatgata tcaatattgg cgctcaggcg acgggagaaa aggacaatca atagcaggct
    10861 tatcagcaaa tcggcggtga gcacaatgat aatcctcaca tccattttcc aggcctggcg
    10921 gcgaatatct tcggttaatt cattggccca gatatagccg aggatttcac cattacgctc
    10981 aatggggagc attgaattca aaatatcgcc gcgcacctgc ctgcctgaat aaaccaaagg
    11041 ggtatttgta cgcatgactt cgcgaccagg gtgatctgcg gcaatggtaa cgccgacatt
    11101 attctgatat agcgctgaag gcgcgtaggt gattatcgca tccagcattt tgttgtaata
    11161 accagcacct atgccaggga aggcgtgagt gatattttcg gtaatggggg caagttctgc
    11221 atttaatgcg cggatacgct cctcacgtgg taagtcgatg tagagatcat agcgatcgcc
    11281 tagtgcctga ttaagcaggt tgaccacggc agaaagtttt ttctcttttt cagataagac
    11341 tgctgaacgt ccttccgttt ctacgatata accaatagta agcgttggga caatgaccat
    11401 caggattgcc atcaggatca tttgattgcg taagcggcgt ggataaatcc acttcatata
    11461 atgcatgctg aaatccacta gtcttgtccg gtatatgacg attatcagag gttaaggtga
    11521 tgatttctcg gcggtgtatc atattccaga gaagagagaa cattgcggta acacgctttt
    11581 accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata
    11641 ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt
    11701 agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca
    11761 gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat
    11821 tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc gcttatcggc
    11881 agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga
    11941 accgctgttt gccgactccg attgttccgc aatgagtaac acctggcgag gttctctgga
    12001 gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctaaa
    12061 ccgggtattt ttaatcggca gcgataacct ctgcatggcc aatttcggtc tgcgtgatat
    12121 gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa cgcatcaata aatatcgaaa
    12181 tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg
    12241 cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc aggcgctttt
    12301 gggtgtcgag cagaccatcc ggatggagaa ctttttctta ccgggtacgt tgccgatggg
    12361 ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagtaa
    12421 aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt
    12481 ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tgtattcggt
    12541 gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa
    12601 tgtgcttgcc ggagctgcat tgtttactct cgcacggatg tacgagcgac gtattttcat
    12661 tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc gcaagattgt
    12721 cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag
    12781 taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacgactgac
    12841 gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca acaataccaa
    12901 tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct
    12961 ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc
    13021 ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac
    13081 gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg
    13141 cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag
    13201 cgatattctc gatttctcga agattgaatc ggaacagttg aagatcgaac cgcgtgagtt
    13261 ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa
    13321 gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc
    13381 gatgcgttta cagcaggtca tctccaacct gttgagtaac gccataaaat tcaccgatac
    13441 cggctgtata gttttgcatg tccgcgctga tggcgattat ctctctatcc gtgttcgcga
    13501 taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt
    13561 cggaacgggc gtacagcgaa acttccaggg gaccggtctg ggtctggcga tttgtgaaaa
    13621 actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa tgggcagcca
    13681 gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg
    13741 gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga
    13801 aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc
    13861 cgaagatgtg ttaattactg acgaggtagt gagtaaaaaa tggcagggta gagcggtagt
    13921 gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca
    13981 cagtgtggct gccccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat
    14041 ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataatga
    14101 cgatatgatg attctggtcg tggatgatca tccgattaac cgacgtctgc tggcagatca
    14161 gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggtgtcgatg cgcttaatgt
    14221 acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa atatggatgg
    14281 ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac
    14341 tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagctgcct
    14401 gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt
    14461 caggaaatcg cgggaatcgt aggacggata aggtgtttat gccgcatctg gcattcagtg
    14521 caaatgccag atgcgacgct gacgcgtctt atctggccta ctttgatgta atctgttgaa
    14581 ataatgggaa tcgtaggacg cataaggcgt ttacgccgca ttcggcaatc ggtgcaaatg
    14641 ccagataaga cactaacgcg tcttatctgg cctacaggtg attagtcttt atctgccgga
    14701 cttaacgtca ctgaagagag ataattcagc agggcgatat cgttctcgac acccagcttc
    14761 atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag ctttttagcg
    14821 atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa cttcactctc ttttggcgag
    14881 agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga aacgctttcc
    14941 ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt tcggcagatc ggtcggtgca
    15001 ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag aatcgccggg
    15061 ttgttgttca tagtcagaac aatgatcgac aggcttggga aatggcgctt gatgtacttg
    15121 attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt aatcaacaca
    15181 tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa ttcgccgaca
    15241 acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac tatcggatgg
    15301 tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct acagcaagct
    15361 cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat ccttctcacg
    15421 aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta gcatggcaaa
    15481 tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg ttgctgcttc
    15541 agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat aatagccgct
    15601 ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct cttcctgcgc
    15661 cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca tattgaagtt
    15721 gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc ttaaaagcaa
    15781 gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat cttgactaat
    15841 taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc gagtgacaat
    15901 attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca gtaaacgctc
    15961 ttcttcctct tcaacttccg ggccagcggc gagcattttg atatgcacag agtagcgtgt
    16021 accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgtg ccagttgatc
    16081 gctcagccag aatgccagcg ggtccgcctt tccataacga tcgttttggg tctggttgat
    16141 aaacgggaag tgcaaattat ccatttcatg aatacttacg ccttctccgg tgtccagaat
    16201 gcggaatgtc aggcggtctt cagaggactc atcctgatca acctcaaggg tgatttttcc
    16261 caattgcgtt gaggtcacgg catattgcat cagtagcagc aaaatacgtc gtaaggcatc
    16321 gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg ttaatcagca gttgcagacc
    16381 tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat cctgcacgga
    16441 gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta actgtatttc
    16501 atcgaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt ccggggcgtt
    16561 gagtttagcc gcgctctccg ccagggactg tgcaggttct ttcagcgcat cgccaatgtt
    16621 tttcataaag gtcatccggc cctgctggtt tttctcatac agacgctgcg cctgcttgag
    16681 tttcttgttt accagcactt cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg
    16741 cgcgacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg tcgcctgaat
    16801 aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat gcggcagcaa
    16861 atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag
    16921 gccgagcggc agcagtgaga ctatcttttc attgattgcc cgtaaaatgc gcaattcgtt
    16981 attgaccgcc gtgttgggta gactttctgt actgcgactg gagaaatggc ggaatgtggt
    17041 atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt
    17101 ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt
    17161 agagttgagt gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc
    17221 cgttccttct ttctcattat cattgtctcc cgttgccgtc gtgtctggct caaggcggaa
    17281 actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacgac
    17341 cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat gaccgttctg
    17401 ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt tggcctgttg
    17461 caacatttct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt taaatccgga
    17521 ggtgagatct ttgaggggca gggttgagat cagtaccaga ctgttatcct gaccattcag
    17581 gtaatacatc gaccacggta cattttctgc accccacaat gtatccagat aagtggacat
    17641 ccgctgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt
    17701 gcggcgtggt ttctccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc
    17761 gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg
    17821 ccaggtatcg acgcgctttt ggattgcgtg ggtaatgtcg acaatctggt aacttttatc
    17881 tttcagccag gcgttaacgg cgctttgtac cattaccccc atcgtcacca gtaacacaat
    17941 gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc
    18001 cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg
    18061 gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga ttctctattc
    18121 tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc attttgctgt
    18181 acaaataacc tacaaaaagc ccaacgaaat taattgcaaa taaacgaaga ttgctggaaa
    18241 ttatgctgat gttaattatt tgtgaaatag ttgacaagag ttatagtttt tctgtggtag
    18301 cacagaataa tgaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt
    18361 gaaatagggg taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga
    18421 tagcgggaga tattctagtt gcgagtgaag gttttgtttt gacattcagt gctgtcaaat
    18481 acttaagaat aagttattga ttttaacctt gaattattat tgct
//
LOCUS       DAJTXQ010000083        18214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000083 DAJTXQ010000000
VERSION     DAJTXQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.083"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            15..1091
                     /locus_tag="OO515_002701"
     CDS             15..1091
                     /locus_tag="OO515_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6576595.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="OO515_002702"
     CDS             complement(1484..1825)
                     /locus_tag="OO515_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6576596.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="OO515_002703"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="OO515_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR6576597.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="OO515_002704"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="OO515_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6576598.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="OO515_002705"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="OO515_002705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6576599.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="OO515_002706"
     CDS             3559..4215
                     /locus_tag="OO515_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6576600.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="OO515_002707"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="OO515_002707"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6576601.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="OO515_002708"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="OO515_002708"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6576602.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="OO515_002709"
     CDS             complement(7167..7826)
                     /locus_tag="OO515_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6576603.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="OO515_002710"
     CDS             complement(8153..8509)
                     /locus_tag="OO515_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6576604.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="OO515_002711"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="OO515_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR6576605.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="OO515_002712"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="OO515_002712"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6576606.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="OO515_002713"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="OO515_002713"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6576607.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="OO515_002714"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="OO515_002714"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6576608.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="OO515_002715"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="OO515_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6576609.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="OO515_002716"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="OO515_002716"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6576610.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="OO515_002717"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="OO515_002717"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6576611.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="OO515_002718"
     CDS             complement(17270..18112)
                     /locus_tag="OO515_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR6576612.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4672 a         4575 c         4391 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc accgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAJTXQ010000084        18187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000084 DAJTXQ010000000
VERSION     DAJTXQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.084"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO515_002719"
                     /gene_synonym="dgcC"
                     /pseudo
     CDS             complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO515_002719"
                     /gene_synonym="dgcC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
     gene            complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO515_002720"
     CDS             complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO515_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6576613.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO515_002721"
     CDS             complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO515_002721"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6576614.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO515_002722"
     CDS             complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO515_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6576615.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3370..3573)
                     /locus_tag="OO515_002723"
     CDS             complement(3370..3573)
                     /locus_tag="OO515_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576616.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3651..4745
                     /gene="ddlA"
                     /locus_tag="OO515_002724"
     CDS             3651..4745
                     /gene="ddlA"
                     /locus_tag="OO515_002724"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6576617.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4769..4981)
                     /locus_tag="OO515_002725"
     CDS             complement(4769..4981)
                     /locus_tag="OO515_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6576618.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5241..5549
                     /locus_tag="OO515_002726"
     CDS             5241..5549
                     /locus_tag="OO515_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6576619.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO515_002727"
     CDS             complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO515_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6576620.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO515_002728"
     CDS             complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO515_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6576621.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8287..9444
                     /gene="ampH"
                     /locus_tag="OO515_002729"
     CDS             8287..9444
                     /gene="ampH"
                     /locus_tag="OO515_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6576622.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO515_002730"
     CDS             complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO515_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6576623.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10156..13044)
                     /locus_tag="OO515_002731"
     CDS             complement(10156..13044)
                     /locus_tag="OO515_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6576624.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13570..14544
                     /gene="hemB"
                     /locus_tag="OO515_002732"
     CDS             13570..14544
                     /gene="hemB"
                     /locus_tag="OO515_002732"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6576625.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO515_002733"
     CDS             complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO515_002733"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6576626.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO515_002734"
     CDS             complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO515_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6576627.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO515_002735"
     CDS             complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO515_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6576628.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO515_002736"
     CDS             complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO515_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6576629.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4534 a         4728 c         4395 g         4530 t
ORIGIN      
        1 tatcaataag ttggagtcat tacccgctac cgcgcagggt aatttgtaac tgtcgggtaa
       61 gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat
      121 ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt
      181 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcgag
      241 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga
      301 cccagccaaa cagcagagga taaacgacaa taatgggaag ggagagccac cattccagcg
      361 gcgcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca
      421 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
      481 acataatcat caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca
      541 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
      601 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
      661 gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc
      721 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
      781 gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa
      841 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
      901 cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag
      961 cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
     1021 acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
     1081 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
     1141 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
     1201 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
     1261 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
     1321 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
     1381 cggcagcgaa aattcactgc cgggcgcggt tttatttcag ccccagagcg gctttcatgg
     1441 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc ggaccatacg
     1501 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
     1561 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
     1621 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
     1681 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
     1741 cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
     1801 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggttttg tcggtcatct
     1861 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct
     1921 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1981 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     2041 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     2101 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     2161 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     2221 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     2281 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     2341 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     2401 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
     2461 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     2521 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     2581 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     2641 gtttatcgct aagagaatca cgcagagcgg cggtctgatc actcgttaag cggcgagcac
     2701 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     2761 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     2821 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2881 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga
     2941 tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg
     3001 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     3061 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     3121 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     3181 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     3241 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     3301 tgtttgatta ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     3361 ttttaattat caacctttat tataaataaa agttatgaaa tatattctct tcgttaactt
     3421 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     3481 gttcatcatt tttgtgatct cgcgcacatc tttgagataa cgcactttct tcaccagtac
     3541 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     3601 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     3661 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     3721 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     3781 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
     3841 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     3901 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     3961 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     4021 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     4081 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     4141 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     4201 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     4261 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     4321 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     4381 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     4441 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     4501 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     4561 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     4621 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     4681 cacgtttgat tgagctggcg ctgaagcgtc acgctgcgga taacgcattg aaagccacaa
     4741 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     4801 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     4861 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     4921 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     4981 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     5041 tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtaactcac
     5101 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     5161 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     5221 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     5281 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     5341 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     5401 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     5461 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     5521 ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc
     5581 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     5641 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     5701 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     5761 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     5821 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     5881 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     5941 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     6001 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     6061 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     6121 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     6181 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     6241 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     6301 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     6361 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     6421 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
     6481 ttccagcgtc ggtgccagcc cctgactttc aaaggtgatg gcgagatctt tcgcccaggc
     6541 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     6601 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     6661 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     6721 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     6781 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     6841 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     6901 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     6961 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     7021 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     7081 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     7141 cagtttgatc cctaccactg ccagccatcc ggtccccatc agcgaccaga cgatagcggc
     7201 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     7261 cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     7321 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     7381 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     7441 gcggaacaca tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     7501 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     7561 cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac
     7621 cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca
     7681 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     7741 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     7801 gatctgcccg gaagcgccgg tgatacgcgc cacccagtct cccccaccgg cttgccagaa
     7861 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg gctttgggaa
     7921 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     7981 taaccgcatg accactacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     8041 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     8101 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     8161 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     8221 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     8281 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     8341 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     8401 gccgatcata ttttttacgg cagcggtgca acggggatgg cgctggttgt tatcgacggt
     8461 aaccagcgcg tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     8521 ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg
     8581 aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca
     8641 ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat
     8701 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     8761 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     8821 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
     8881 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
     8941 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
     9001 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     9061 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     9121 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     9181 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     9241 gagggtcgtc cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
     9301 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
     9361 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
     9421 ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     9481 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     9541 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     9601 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
     9661 atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccaag ctaaccagta
     9721 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag
     9781 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     9841 accatcagcc agccccataa tataaggggc ctgggtaata ccgataagta cgttttcttc
     9901 tctacgcaga gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact
     9961 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
    10021 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
    10081 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct
    10141 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
    10201 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
    10261 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
    10321 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
    10381 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
    10441 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
    10501 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
    10561 tttgaagtcg taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc
    10621 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
    10681 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
    10741 gctgtcttga tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
    10801 cgcaccgacg atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat
    10861 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
    10921 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
    10981 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
    11041 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
    11101 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
    11161 tttggtatca tcgatataga tgagctgctt acctgcgtaa tcagcaatcg caccagcgcc
    11221 agtggagttg tcgatacgaa ctttgtagtt accggttgca gctacaacgt tgtctgcaga
    11281 gctgttgtca acttcgtcgc cattgttaat gtcgttgtta ccattaacgg tcaagtgacc
    11341 atcagagttc atcgcaataa caccataacc gtagttagct ttgctggtgt cgttggtacg
    11401 atcgttgacg aggtctgcat tcaggatgta atcattacta tgaatgtcaa acacacctgc
    11461 gtaaacatta ccgttgttat ctttaacgtt gccactggtc agttccagag tatcagttgc
    11521 aaatacgctg gagatgtcat tcatgttggt gtttacatcc agcacaccac cgttagtaac
    11581 ggtaatggta ttggcataca gcggtgcata tgcccaagct gaagcagtat cttcaccgaa
    11641 gttaacggtt gagaaggtat caatggtcag gtgatcggtg ttaacttcac cgttaacttc
    11701 cattgaagaa ccaccgttca gagtgatggt gtcagaagtc agagaagaat ctgcactcac
    11761 gttaacctga gaaccgctgt tgatcgccag ggtatcgatc agggaagttt tcgtggtttc
    11821 ccaatcagaa ccgttgttca gaacaacatc gaatacgccg ctctggaatg cgttagcgtt
    11881 atcaacacga ttccagccat cttcagagta agaagcgtac gggttcaggc tgtttggtgc
    11941 aacgtcgtag aaggcttcgt cagcaacaac aacgtcataa tcaatattcg ctgaaccaac
    12001 ccatttggtg ttgttcagag tcaggttcag gtgatcgacg ttctgatcgt cagttgccca
    12061 accgccatga gtgtaagcag tggtagagtt gtcaatcagg tgacccgtcg cgtcaaagtt
    12121 agcgttccag gtactgtcaa atacaacgtc accaagcaac tgagagttgt tgaacgtaac
    12181 gttgttgacc atacgataat cagaagtgtc atcatcgatg aaggctaacg caacatcatc
    12241 agcaggagat gcgccattgc cgtagtcact tggttcaaca gaattgccga aatgaccacg
    12301 atctgattgt tcgtcttcag tccatgaacc agaagttaca gtagagtcat taacaacgat
    12361 gttgttgtcc cactgatgac cttcggtatt gtaggtcgca ttaccgaggt cgtgttgaac
    12421 caaagtaata cctgcaacat gggagttgtt gctgatgttg atgttacttt caacatccag
    12481 agtcacagcg gtaccaagac cagcggttac caaacgatca taatctgtct tagatgtttt
    12541 accgtctgca tccagataag tatcggtgaa gtagaaggcg tcaaaatcat cgtcaatagt
    12601 agaattagcg atgttcagag tgaagatgtc gccatcaacc cagtcgtggc cagtataatc
    12661 gctaccgttg gtccagacgt agtcgccata accaacctgg taagatgtaa ccataccatg
    12721 aacaattgag ttggagatgt tcagtgtatt accggtatct gtgccataat cgtggttcag
    12781 ataataagta gaaattacac catttacagt gtcgccgctg aaagtggtat caaagtaacc
    12841 atcagctgcg tggtcatata cataaccacc gtaagtcaga taatcgtgat caattttagt
    12901 gctgtcaccc caattgtaga tggtggctgc gtcatcgtga tacccgaagg tgttataagt
    12961 agctgcatta gcctgagagg tgatagccag agtgcaagcc aatgctaatt gtgatactac
    13021 aagtttcttt ttccaggagt gcatttgtca tccctcctca gggacgtaat aagttgatcc
    13081 atcaattatc aatgcataga aaacatatta tgtttcgcgc taacgattat gctgcgttac
    13141 agacgaatgg ttcaattatt ctttccccta agtccgaatg tggacatata taatttatgg
    13201 agaaaaacac gcatcaaatt gataaaagga cgatatttca acatgttgca gataatgtta
    13261 tttttgatac atacgcgtta taaatatcaa aaacacaaac cagataaata tcttatcaat
    13321 atacaacaaa gtttgaatta ttcaatttat agcagcacaa ccaaacgcca aaaaaaataa
    13381 taaccaagag ctataaataa agattaaaaa tattttcagt gaatatgaaa ttattgctat
    13441 ttctattaca aagatgtcct ggtctctctt tcattctctt cttgtcaaca tcacgacaac
    13501 tttcgtaaaa cactcctgcc ccgcttcagg tatactgtgc accttaattc cacaaacatc
    13561 aggcagacca tgacagactt aatccaacgc cctcgtcgcc tacgcaaatc tcctgcgctg
    13621 cgcgctatgt ttgaagagac aacacttagc cttaacgacc tggtgttgcc gatctttgtt
    13681 gaagaagaaa ttgacgacta caaagccgtt gaagccatgc caggcgtgat gcgcattcca
    13741 gagaaacatc tggcacgcga aattgaacgc atcgccaacg ccggtatccg ttccgtaatg
    13801 acttttggca tctctcacca taccgatgaa accggcagcg acgcctggcg ggaagatgga
    13861 ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc cagaaatgat cgtcatgtca
    13921 gacacttgtt tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc
    13981 gtcgacaacg acgcgactct ggaaaattta ggcaagcaag ccgtggttgc agctgctgca
    14041 ggcgcagact tcatcgcccc ttccgccgcg atggacggcc aggtacaggc gattcgtcag
    14101 gcgctggacg ctgcaggctt taaagatacg gcgattatgt cgtattcgac caagttcgcc
    14161 tcttcctttt atggcccgtt ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa
    14221 agctatcaga tgaacccaat gaaccgtcgc gaggcgattc gtgaatcact gctggatgaa
    14281 gcccagggcg cagactgcct gatggttaaa cctgctggag cgtacctcga catcgtgcgt
    14341 gagctgcgtg aacgtactga attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg
    14401 atgattaagt tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc
    14461 ttaggttcaa ttaagcgtgc gggtgcggat ctgattttca gctactttgc gctggatttg
    14521 gctgagaaga agattctgcg ttaatttttt tcctctcaga aagggggagt taacctgttg
    14581 aaagtacggt gaacgccccc tcaccctaac cctctcccca agggggcgag ggaaccgtcc
    14641 gcactcgtat caccccgccc gataaaacgg tttatcccca aggatcgtcg cccgatgcat
    14701 aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc tgagtcacgc ggttatccca
    14761 aatcgcaata tcattcggtt gccagcgcca acgcacctga aactccggtt tagtgatatg
    14821 ggcgaataaa aagcctaaca aggcttcgct ctctttctcg ctgacatcga caattcgcgt
    14881 agtaaagcct tcattcacaa acagcgcctg tttaccgctc accggatgcg ttcgcaccac
    14941 cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc
    15001 ggttttgcgg tatttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcagccc
    15061 gctcagcagc tggcggaagg gaacagagag cgcctcatag gccgcaatac cgctggtcca
    15121 gagcgtatca ccgccggtcg aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg
    15181 cgtttcaata aatgtcacat cggtatgcca gttgtcgtta tccggcggat tatcgttatg
    15241 ggtatccagc acgatgatct cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg
    15301 cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg
    15361 cagaaatacc acctgatagc gcagcaccgc atggtaaagc tgttcaaact gattatcgct
    15421 taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg atatacggcc ccagcggggt
    15481 aatactcaga cgttcactca ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac
    15541 gcgcgcagac ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt
    15601 accacgtcag tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgtgtc
    15661 gcggcaatca gctccgccgc taccagcgta gaccagccca cacccagacc aatgcgtaat
    15721 ccggtgagga tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg
    15781 ctggcaccca gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc
    15841 agcaccgaca tcgccaccgg tgcaaaaatc gctaaataga tcaataagat cttcgaggtt
    15901 tcaccaatac caaaccagat caccatcagc ggcaaataag ccagcggcgg caccggacga
    15961 taaagttcga ttaccggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc
    16021 ccgaccggaa taccgatcac caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg
    16081 gctgccagat gctgccacag cgtggcgtcc ataaatccct gcggtccggc aatggtgagc
    16141 agttttgcca gtacttgttg cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc
    16201 gccaccgtcc accatacagt gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga
    16261 cgcgagagcg gccagcgcca tttcagccgc cgcgaatgca gtttttcatt aatgagcaca
    16321 ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga
    16381 tgaattgtgg atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa
    16441 agttgagcgg cagccgctcc agcacacggc cagggccgga tgaaagcaga accagttcag
    16501 tcgccataaa caccgcttct tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct
    16561 cctgccagag tttcagcagc agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac
    16621 caaacggttc gtcgagtaat aacagctggg gattcgccgc cagcgcgcgg gcaatcccca
    16681 cccgctgacg ttgaccaccg gaaagctgcc agatatagcg tttttctgcg ccttccagcc
    16741 ccactttttt cagcatctgg tgcgcgattt ccagtcgctg cattttctct atacctgcca
    16801 attgcaggcc gaacgccacg ttgtcctgta cattgcgcca cggtagtagc ccttcattct
    16861 gaaaaaccac cccgcgctct gctcccggtc cctcaatacg cttacccgcc agttgaatgc
    16921 tgccatgctg ataaggcaca aaaccggcaa tcagattcag cagggtggtt ttaccgcagc
    16981 cggacggccc cagcaccacc agtagctcgc cgctttccag cgtcaggttg atatcttcca
    17041 gtgccggttt gccgccataa tcggcataga gatgagagat ttgcagcatc tgcgcctcct
    17101 tttattgcac gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggacct
    17161 tgccctgctc tttcaaaaac tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca
    17221 gttctgccgt ttgttgctgc ggcgtcagat aggtattccc cttcaccagc cccggaacgt
    17281 caccttccgg cacgccgctt aaacgcgcca gtttgctgat gttttccggc tgtttcagcc
    17341 acgcgtctgg gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt
    17401 tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac cacccagacg tccagcgttg
    17461 gcgcacccca ctgcccgacc tgttcagaat cggtcaacac cttgccgtct ttttccaggg
    17521 cgttaaccgc cggtgcccag acataagcac catcaatatc accccgctgc catgcggcga
    17581 taatcgcggg cggctgcagg ttcacaatct ccacttgccc tggtttaatc ccccagtgtt
    17641 tcagcgccgc cagcaggctg tagtgggtgg tggagataaa cggtacggca atgcgtttgc
    17701 caatcaaatc ttcgggtttg ctgatagttt tctttaccac cagcgcttcg gagttaccca
    17761 gttttgacgc cagcaagaag acttcaatcg gcacctgttg gctggctgca accgctaatg
    17821 ggctggaacc gaggttgccg atttgcacgt cgccagaagc cagcgcccgc acgatgctgg
    17881 ctccgctgtc aaacttacgc cagtccacgc tcgccccgct ctctttagca aaggtgttgt
    17941 cggcctgagc cactttcgcc ggttcggctg aggtttgata cgccacggtg acgttcaccg
    18001 cctgtgcctg aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg
    18061 ccatgattgt ctgctcccct gtcttgttat gggagcagta ttcaggaata aaaacattca
    18121 ttaaaagaat tagtcgttat cgcacagatg attttattct tagcaaaaaa acggcaaagg
    18181 aaagatt
//
LOCUS       DAJTXQ010000085        17853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000085 DAJTXQ010000000
VERSION     DAJTXQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.085"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO515_002737"
     CDS             complement(<1..304)
                     /locus_tag="OO515_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6576630.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(344..481)
                     /locus_tag="OO515_002738"
     CDS             complement(344..481)
                     /locus_tag="OO515_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576631.1"
                     /translation="MLFLYYAVERKVVSKGLRSREMADAGNFGGSVPERKRNTVLSQP
                     L"
     gene            complement(514..1032)
                     /locus_tag="OO515_002739"
     CDS             complement(514..1032)
                     /locus_tag="OO515_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6576632.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OO515_002740"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OO515_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCR6576633.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OO515_002741"
     CDS             2264..2488
                     /locus_tag="OO515_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576634.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OO515_002742"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OO515_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OO515_002743"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OO515_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6576635.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OO515_002744"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OO515_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6576636.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMQPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OO515_002745"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OO515_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCR6576637.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OO515_002746"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OO515_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCR6576638.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OO515_002747"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OO515_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCR6576639.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OO515_002748"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OO515_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OO515_002749"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OO515_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCR6576640.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="OO515_002750"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="OO515_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14047..14790
                     /gene="tagO"
                     /locus_tag="OO515_002751"
     CDS             14047..14790
                     /gene="tagO"
                     /locus_tag="OO515_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCR6576641.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14795..16207
                     /gene="tssA"
                     /locus_tag="OO515_002752"
     CDS             14795..16207
                     /gene="tssA"
                     /locus_tag="OO515_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCR6576642.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16226..>17853
                     /gene="tssM"
                     /locus_tag="OO515_002753"
     CDS             16226..>17853
                     /gene="tssM"
                     /locus_tag="OO515_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCR6576643.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4154 a         4896 c         4878 g         3925 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgca gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    14161 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    14221 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    14281 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    14341 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    14401 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    14461 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    14521 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    14581 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    14641 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    14701 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    14761 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    14821 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    14881 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    14941 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    15001 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    15061 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    15121 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    15181 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    15241 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    15301 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    15361 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    15421 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    15481 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    15541 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    15601 tagccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    15661 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    15721 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    15781 ggcagacgcc atccacgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    15841 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    15901 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    15961 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    16021 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    16081 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    16141 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    16201 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    16261 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    16321 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    16381 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    16441 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    16501 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    16561 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    16621 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    16681 gctgcgcgag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    16741 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    16801 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    16861 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    16921 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    16981 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    17041 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    17101 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    17161 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    17221 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    17281 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    17341 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    17401 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    17461 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    17521 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    17581 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    17641 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    17701 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    17761 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    17821 actgtaccag ggacgacgta tcgggcctta tgt
//
LOCUS       DAJTXQ010000086        17668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000086 DAJTXQ010000000
VERSION     DAJTXQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.086"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="OO515_002754"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="OO515_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6576644.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="OO515_002755"
     CDS             737..1042
                     /locus_tag="OO515_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576645.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="OO515_002756"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="OO515_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6576646.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="OO515_002757"
     CDS             1790..2536
                     /locus_tag="OO515_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6576647.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="OO515_002758"
     CDS             2536..4632
                     /locus_tag="OO515_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6576648.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="OO515_002759"
     CDS             4678..5817
                     /locus_tag="OO515_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6576649.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="OO515_002760"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="OO515_002760"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6576650.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="OO515_002761"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="OO515_002761"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6576651.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /gene="appA"
                     /locus_tag="OO515_002762"
     CDS             complement(8566..9864)
                     /gene="appA"
                     /locus_tag="OO515_002762"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6576652.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="OO515_002763"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="OO515_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6576653.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="OO515_002764"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="OO515_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6576654.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="OO515_002765"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="OO515_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6576655.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="OO515_002766"
     CDS             complement(12875..13732)
                     /locus_tag="OO515_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6576656.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="OO515_002767"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="OO515_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6576657.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /locus_tag="OO515_002768"
     CDS             complement(14124..14711)
                     /locus_tag="OO515_002768"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6576658.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="OO515_002769"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="OO515_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="OO515_002770"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="OO515_002770"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6576659.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..>17668)
                     /gene="hyaA"
                     /locus_tag="OO515_002771"
     CDS             complement(17223..>17668)
                     /gene="hyaA"
                     /locus_tag="OO515_002771"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6576660.1"
                     /translation="FYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGP
                     TTYNACSSTRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTV
                     GLTALGVVAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4358 a         4680 c         4545 g         4085 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 tatcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg tgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga tacgctgacc ataaaaca
//
LOCUS       DAJTXQ010000087        17633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000087 DAJTXQ010000000
VERSION     DAJTXQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.087"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="OO515_002772"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="OO515_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6576661.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="OO515_002773"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="OO515_002773"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6576662.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="OO515_002774"
     CDS             4390..4641
                     /locus_tag="OO515_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6576663.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="OO515_002775"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="OO515_002775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6576664.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="OO515_002776"
     CDS             5946..6704
                     /locus_tag="OO515_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6576665.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="OO515_002777"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="OO515_002777"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6576666.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="OO515_002778"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="OO515_002778"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6576667.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="OO515_002779"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="OO515_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="OO515_002780"
     CDS             complement(9558..10178)
                     /locus_tag="OO515_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6576668.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="OO515_002781"
     CDS             complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="OO515_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6576669.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="OO515_002782"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="OO515_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6576670.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="OO515_002783"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="OO515_002783"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6576671.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="OO515_002784"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="OO515_002784"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6576672.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="OO515_002785"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="OO515_002785"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6576673.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="OO515_002786"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="OO515_002786"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6576674.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..>17633
                     /gene="trpA"
                     /locus_tag="OO515_002787"
     CDS             17054..>17633
                     /gene="trpA"
                     /locus_tag="OO515_002787"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6576675.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPL"
BASE COUNT         4229 a         4675 c         4412 g         4317 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tca
//
LOCUS       DAJTXQ010000088        17448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000088 DAJTXQ010000000
VERSION     DAJTXQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.088"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..502)
                     /locus_tag="OO515_002788"
     CDS             complement(<1..502)
                     /locus_tag="OO515_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6576676.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OO515_002789"
     tRNA            complement(688..772)
                     /locus_tag="OO515_002789"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OO515_002790"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OO515_002790"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6576677.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO515_002791"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO515_002791"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6576678.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OO515_002792"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OO515_002792"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCR6576679.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OO515_002793"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OO515_002793"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR6576680.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OO515_002794"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OO515_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCR6576681.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OO515_002795"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OO515_002795"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCR6576682.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OO515_002796"
     CDS             7117..8619
                     /locus_tag="OO515_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6576683.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO515_002797"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO515_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6576684.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO515_002798"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO515_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6576685.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OO515_002799"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OO515_002799"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6576686.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OO515_002800"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OO515_002800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6576687.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OO515_002801"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OO515_002801"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6576688.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OO515_002802"
     CDS             complement(16186..17382)
                     /locus_tag="OO515_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6576689.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4484 a         4335 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAJTXQ010000089        17294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000089 DAJTXQ010000000
VERSION     DAJTXQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.089"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            93..1133
                     /locus_tag="OO515_002803"
     CDS             93..1133
                     /locus_tag="OO515_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6576690.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OO515_002804"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OO515_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6576691.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OO515_002805"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OO515_002805"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6576692.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="OO515_002806"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="OO515_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6576693.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAQMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OO515_002807"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OO515_002807"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6576694.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="OO515_002808"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="OO515_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6576695.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9087)
                     /locus_tag="OO515_002809"
                     /pseudo
     CDS             complement(8440..9087)
                     /locus_tag="OO515_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OO515_002810"
     CDS             complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OO515_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6576696.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OO515_002811"
     CDS             complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OO515_002811"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6576697.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OO515_002812"
     CDS             complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OO515_002812"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6576698.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12900..14081)
                     /locus_tag="OO515_002813"
     CDS             complement(12900..14081)
                     /locus_tag="OO515_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6576699.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14336..15565
                     /locus_tag="OO515_002814"
     CDS             14336..15565
                     /locus_tag="OO515_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6576700.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16025..16657
                     /locus_tag="OO515_002815"
     CDS             16025..16657
                     /locus_tag="OO515_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6576701.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16991..>17294
                     /locus_tag="OO515_002816"
     CDS             16991..>17294
                     /locus_tag="OO515_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6576702.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4580 a         4512 c         3975 g         4227 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgccta ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacatggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggtacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgcccaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tattccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
     8881 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
     8941 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
     9001 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
     9061 tcctcccagc aaaccaattg ttttcattcc tctctctcct gatgtgtgtt accgcaacaa
     9121 ggctgaataa ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa
     9181 aaaagggccg gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact
     9241 tctcgcctgc catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa
     9301 tgagccagaa agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac
     9361 cggcagcacc aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca
     9421 cccagttcgt ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt
     9481 cagagcacag gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca
     9541 tcgtcatgcc aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca
     9601 tcaggcaata gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag
     9661 ccggtttacg ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg aacataaagg
     9721 tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt
     9781 tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct
     9841 gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc
     9901 aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt
     9961 ccgacgtatc gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg
    10021 ccagccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta
    10081 aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata
    10141 aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc
    10201 gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga
    10261 cagcaatgcc cagcaccacc cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg
    10321 accctataga accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca
    10381 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10441 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10501 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10561 aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg
    10621 tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg
    10681 aacaactcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10741 tgtcgttatg tgatggatat tccaattttc aaattaagtt gaattattga gattattatt
    10801 aaccacctaa ttttacagca gataaaattc ataaagttca ttaattgata attgatatgg
    10861 attatttcat aaccatgata tgaattatga tgacctacag gtataaaaaa ccctgccatg
    10921 aggcagggtc ataaaagtaa gaggaatgaa ttaacgagcc agccaaccgc catctacggc
    10981 aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta tcggccccat
    11041 caggtcactc ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt ccgcgctacg
    11101 ttgttcatct gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc
    11161 attaacatta atgttgtgtt tagcccattc gttcgccatc aatcgcgtca cgcccatcac
    11221 gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11281 cgtgatattg ataatcttgc cgccattgcg ttgcgcgata aagtgtttcg ccgctgcctg
    11341 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11401 gaactcgaga gcatcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11461 accaaactca gctaccgcgc gatccagcag tgctggaatg ccatcaatct ttcgcagatc
    11521 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg
    11581 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11641 ctggcccagt ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc
    11701 acttaaaatc ataacaatac cttattcgtt tatgccgaca actagcgcaa atctttaacg
    11761 gccactatgc cgacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
    11821 cctggttttc accaaccatg ccccagataa aggtataagc tttggttccg acgccggaat
    11881 ggatcgacca gctgggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
    11941 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
    12001 aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag ttacctggag
    12061 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
    12121 tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
    12181 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
    12241 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
    12301 acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc gtaccggcac
    12361 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
    12421 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
    12481 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
    12541 aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
    12601 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
    12661 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
    12721 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
    12781 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
    12841 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
    12901 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
    12961 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
    13021 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
    13081 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
    13141 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
    13201 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
    13261 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
    13321 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
    13381 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
    13441 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
    13501 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
    13561 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
    13621 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
    13681 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
    13741 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
    13801 gctttaagtc cggaaccgca aacgtcatta atcgtgattg cagaaacgct attaggaaga
    13861 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
    13921 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
    13981 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
    14041 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
    14101 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
    14161 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
    14221 gcaaccacta acccaccaat agatgggtag ataaggcgtt aatctcccat acttaacctg
    14281 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
    14341 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
    14401 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
    14461 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
    14521 cctctcatca aaaacatcag catgtgaagg gataacgggc gcggtaacac actactatgg
    14581 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
    14641 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
    14701 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
    14761 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    14821 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
    14881 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    14941 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    15001 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    15061 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    15121 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
    15181 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    15241 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
    15301 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    15361 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
    15421 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
    15481 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
    15541 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
    15601 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
    15661 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
    15721 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
    15781 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
    15841 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atgtcttaat
    15901 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaaga
    15961 tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct
    16021 taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg
    16081 ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt gtttttgtat
    16141 gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct taacaaaaaa
    16201 ccacaatgcg agattaagcg gatatatttt tgtggatttt tcagtaagtt ttttacgtct
    16261 attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga tagttttagt
    16321 cagcgaccgc aatatgcaat cgttagctaa ctactggcga aaacacaaca ccgcaatatc
    16381 tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca gacaactatt
    16441 tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg aattcaccat
    16501 aatggggtat atggtttctg gttataatcc atatcagatt gctgaagtgc tggatatgga
    16561 tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa
    16621 cgaattattt attcgttcac attcggtcca acattgatac tcaacgacca gccagaatca
    16681 tactctgttc atacgggcat ctattgaaat gcccgtattt tataaataag cagtaacccc
    16741 aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat
    16801 atgaatttac aaacaatagt ggcataaatg ttaaccatgt taatttacgt aaagttttac
    16861 gttgcaacat taaagcctca tttcaatcat catgataaat ataaaattaa tatatattta
    16921 tgccgtaaat accaatatac ttagcaaact atgtgatctc catttcgatt gatttagtgt
    16981 ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg agcaccgaaa
    17041 attaatatcg gtacataacg ccgatcttaa cgtcgttctg acaacgccag ccagtcgatg
    17101 tttatcactt ctacttgaag cttttcctga tgtggttgca caacaagatt ttttcaccag
    17161 agtctgggaa gaagaaggta tgcgtgtgcc tactaacacg ttatatcaga acatatccat
    17221 tatcagacgc ggatttcgcg ctgttggtga tactacccac tcgctaattg caaccgtgcc
    17281 gagagaggat tcaa
//
LOCUS       DAJTXQ010000090        17253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000090 DAJTXQ010000000
VERSION     DAJTXQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.090"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OO515_002817"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OO515_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6576703.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OO515_002818"
     CDS             complement(2362..2802)
                     /locus_tag="OO515_002818"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6576704.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OO515_002819"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OO515_002819"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6576705.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OO515_002820"
     CDS             3520..4218
                     /locus_tag="OO515_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6576706.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OO515_002821"
     CDS             4447..5655
                     /locus_tag="OO515_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6576707.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OO515_002822"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OO515_002822"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6576708.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OO515_002823"
     tRNA            7762..7838
                     /locus_tag="OO515_002823"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="OO515_002824"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="OO515_002824"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="OO515_002825"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="OO515_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6576709.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="OO515_002826"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="OO515_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6576710.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="OO515_002827"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="OO515_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6576711.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="OO515_002828"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="OO515_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6576712.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="OO515_002829"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="OO515_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6576713.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="OO515_002830"
     CDS             complement(14622..15893)
                     /locus_tag="OO515_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6576714.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16369..16476
                     /locus_tag="OO515_002831"
     CDS             16369..16476
                     /locus_tag="OO515_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6576715.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16852..16959
                     /locus_tag="OO515_002832"
     CDS             16852..16959
                     /locus_tag="OO515_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6576716.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4113 a         4287 c         4674 g         4179 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    16201 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    16261 cccccgcacg ttgcaggtat gtacctgaca acataacggg ggctaatctt gaacctagac
    16321 cactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
    16381 gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
    16441 agtatgatcg tgaactggct gaacaagcgg aagtagcgtg tcatgtgggc gtcaggctgc
    16501 cgtaatggca atttgcgccc ggaccaggcc gcaggagtga aactctgcgg cctttttcgt
    16561 aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa
    16621 gatgagacgg attccggaac agaaaaacag tcgtagaatc agcgtcaagc cggaaggatt
    16681 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga
    16741 aaacccccgc acgttgcagg tatgcacctg acaacaccac gggggccatt cttgcatcca
    16801 aacaatacaa gaatagccgc gaaacgttgt cattacaata caggcggcta tatgacgctc
    16861 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggtattctt
    16921 gccagtatga tcgtgaactg gctgaacaag cggaagtaac gtgccatgcg ggcgtcaggc
    16981 tgccgtaatg gcaatttgcg cccggatcag gccgcagggg tgaaactctg cggccttttt
    17041 cgtaagaaaa atgcaacggt tgcaagcgga taatattctg cggaatgcgc agcacagagt
    17101 gaagacgtga cggattccgg aacagaaaaa cagtcgtaga atcagcgtca agccggaagg
    17161 gttccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt tgctggagag
    17221 agaaaacccc cgcacgttgc aggtatgcac ctg
//
LOCUS       DAJTXQ010000091        16636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000091 DAJTXQ010000000
VERSION     DAJTXQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.091"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(99..2540)
                     /gene="rnr"
                     /locus_tag="OO515_002833"
     CDS             complement(99..2540)
                     /gene="rnr"
                     /locus_tag="OO515_002833"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6576717.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2579..3004)
                     /gene="nsrR"
                     /locus_tag="OO515_002834"
     CDS             complement(2579..3004)
                     /gene="nsrR"
                     /locus_tag="OO515_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6576718.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3209..4507)
                     /gene="purA"
                     /locus_tag="OO515_002835"
     CDS             complement(3209..4507)
                     /gene="purA"
                     /locus_tag="OO515_002835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6576719.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4611..4808)
                     /locus_tag="OO515_002836"
     CDS             complement(4611..4808)
                     /locus_tag="OO515_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6576720.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4890..5894)
                     /gene="hflC"
                     /locus_tag="OO515_002837"
     CDS             complement(4890..5894)
                     /gene="hflC"
                     /locus_tag="OO515_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6576721.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5897..7156)
                     /gene="hflK"
                     /locus_tag="OO515_002838"
     CDS             complement(5897..7156)
                     /gene="hflK"
                     /locus_tag="OO515_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6576722.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7242..8522)
                     /gene="hflX"
                     /locus_tag="OO515_002839"
     CDS             complement(7242..8522)
                     /gene="hflX"
                     /locus_tag="OO515_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6576723.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8599..8907)
                     /gene="hfq"
                     /locus_tag="OO515_002840"
     CDS             complement(8599..8907)
                     /gene="hfq"
                     /locus_tag="OO515_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6576724.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8993..9943)
                     /gene="miaA"
                     /locus_tag="OO515_002841"
     CDS             complement(8993..9943)
                     /gene="miaA"
                     /locus_tag="OO515_002841"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6576725.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9936..11783)
                     /gene="mutL"
                     /locus_tag="OO515_002842"
     CDS             complement(9936..11783)
                     /gene="mutL"
                     /locus_tag="OO515_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6576726.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11793..13130)
                     /gene="amiB"
                     /locus_tag="OO515_002843"
     CDS             complement(11793..13130)
                     /gene="amiB"
                     /locus_tag="OO515_002843"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6576727.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13149..13610)
                     /gene="tsaE"
                     /locus_tag="OO515_002844"
     CDS             complement(13149..13610)
                     /gene="tsaE"
                     /locus_tag="OO515_002844"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6576728.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13582..15129)
                     /gene="nnr"
                     /locus_tag="OO515_002845"
     CDS             complement(13582..15129)
                     /gene="nnr"
                     /locus_tag="OO515_002845"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6576729.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15128..16267
                     /gene="queG"
                     /locus_tag="OO515_002846"
     CDS             15128..16267
                     /gene="queG"
                     /locus_tag="OO515_002846"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6576730.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16250..16303)
                     /locus_tag="OO515_002847"
     CDS             complement(16250..16303)
                     /locus_tag="OO515_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576731.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(16537..16612)
                     /locus_tag="OO515_002848"
     tRNA            complement(16537..16612)
                     /locus_tag="OO515_002848"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16577..16579),aa:Gly,seq:gcc)
BASE COUNT         3767 a         4756 c         4181 g         3932 t
ORIGIN      
        1 tgattatgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcgcattttg
       61 tcagcaatct aaccctcttc ttttaaagag ggtattgatc actctgccac ttttttcttc
      121 gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga tggctttttc
      181 gcttttctcg cgtctttctt cgccgctttc ggcttcgttt ttttctcacc gcggaaggcg
      241 ctgtctggct caaagtttac ctttttaccg acctgacgac gcttgccgcc ttttttgcct
      301 gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttca
      361 ctggagatca gactaaagtc gattttgcgc tcgtccatat taaccgcttc gacgcgaact
      421 tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cccccatcag gcgttgccct
      481 acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag accatcaatg
      541 aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat tacgccttta
      601 aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc gacatcgcgc
      661 gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg caacatctct
      721 tccatcgaat aatggtagcc gccggtttca gtggtgttgc cctgatgccc ctgctctttc
      781 gccagcagat acttaatggc gcggtgcagc gtcaggtcag gataacgacg aatcggcgaa
      841 gtaaagtgcg cataggactg caatgccagg ccaaagtgac cacggttttc tggatcgtaa
      901 atcgcctgtt tcatcgagcg cagcagcatg gtttgcagca tttctgcatc aggacggtcg
      961 gcaaccgact ccagcagttc cgcgtagtca cgcggttccg gcttgttacc acccggcagc
     1021 tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg
     1081 tcgtgaatac ggaacagtgc cggttctttc gctttctcaa cgaaacgcgc cgccgagata
     1141 ttcgccagaa tcatgcactc ttcaattaat ttgtgcgcgt cgttacgctg ggtctgttcg
     1201 atacgttcaa tacggcgttc agcgttgaaa atgaacttcg cttcttcgct ctcaaatgag
     1261 atcccaccgc gttcttcacg ggctttatcc agcactttat agaggttatg caactcttcg
     1321 agatgcttaa ccagcgggac gtactgctcg cgcagatcct gatcgccctg cagaatatgc
     1381 cagactttgg tgtaggtcag acgcgcgtga gagctcatca ccgcttcgta gaatttgtag
     1441 cccgtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag gcggtctacc
     1501 tgcgggttga gcgaacacag gccgttagag agcacttccg gcagcatcgg gataacctgc
     1561 gaagggaagt acaccgacgt gccacggtta cgcgcttctc tgtccagcgg cgttggcgga
     1621 cgcacatagt agctgacgtc ggcaatcgcg acccataaac gccagccgcc gccgcgtttt
     1681 ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc
     1741 ggtaaatcgc gcaaatcgac acggcccgct tttgcttctt ccggcacttc ttctttcagc
     1801 cccgcaacct gttgctcaac agcctgcggc cagatatacg gaatttcatg ggtacgcaga
     1861 gcgatatcaa ccgccatgcc ggtgcccata ttgtcgccca gcacttcgac gattttaccc
     1921 accgctttgg tgcggcgagt cggacgctga gtcagttcga ctacgaccac aaagcccatc
     1981 cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg gctatcgtca
     2041 ggaaccacaa agccgacgcc cgcttcggta aagtagcgac caacaatctg gctggttttt
     2101 ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc acccagcggc
     2161 tgcgccagca cctgatcgcc atgaatacag gttttcatct gctcgctgga gagatacaaa
     2221 tcatctttac gcccttcaac ccgcagaaag ccgtagccat cacggtggcc aataacggta
     2281 cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacgacgagt gaagaccagt
     2341 tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg ctcttcgcct
     2401 tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg tttggttaaa
     2461 tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc ttcgcgttcc
     2521 tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc
     2581 actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt
     2641 agttatccag ttccgtaaga aaactttgca cggccttaga aagcgcctgt ttcaaccgac
     2701 aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag ggctccagct
     2761 cgcgcaccac atcaccaata cgtatcgcac tcgccggttt acccaggcga atgccgccat
     2821 tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt attttgacca
     2881 tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc atccgccctt
     2941 ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa ctcgttaact
     3001 gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt tatattgatg ataaaccagc
     3061 cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag gatttaacac
     3121 tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt tagcccggca
     3181 ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cggatcgcgc agaatcatgg
     3241 tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccggtc agctcttcaa
     3301 tacgcttgat gtagttcagc gccgcctgcg gcaggccgct acgatctttc acgccgaaag
     3361 tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc cagtcgtcag
     3421 ctgccagcgg agtggtagcc acttcgcgac catccggcat acggtaagcc acgcagagtt
     3481 taacctcttt caggccatcc agaacgtcca gcttggtcag gcaaaagcca gacagggagt
     3541 tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga cgacgacgac
     3601 ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca
     3661 gttcggtcgg gaacggacct gcacctacac gagttgagta agctttgagg atacccagaa
     3721 cataatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca gcagtggtgt
     3781 tggaagaagt tacgtacgga taagtaccgt ggtcgatatc cagcagcgta ccctgcgcac
     3841 cttcaaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa
     3901 ccaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact ttctggtaat
     3961 caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc atcacttctt
     4021 tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg
     4081 ctactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc
     4141 cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc agcggacatg
     4201 cttcagacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt tctttcatct
     4261 ctttcatcag cgcggccgga gacagcacaa caccgttacc gatgatgctg gttacattct
     4321 cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg attacgagag
     4381 tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt tcagtcagaa
     4441 gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg acgacgttgt
     4501 tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc tttttcagag
     4561 ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc
     4621 gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc gccaaaacga
     4681 cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca tgccttcggg
     4741 taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag ccagattgtc
     4801 gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag gatgcggtgg
     4861 ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg
     4921 tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc agagaagctg
     4981 ttctcataag cacgcaggct acggatgaat gcgtagaagt ccggatcttt actgaatgca
     5041 tcagcaaaca gtttggctgc ttcggcatcg ccttcaccac gcatgatgcg gccctgacgc
     5101 tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag tttttccgct
     5161 tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc gcgcatacgg
     5221 ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttgatacg cacatcgaca
     5281 acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt gcccttcgtc
     5341 tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgccgccgg ggtagtaact
     5401 tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag ggtcagacga
     5461 ccacgggaat cggtgacgat gtctttcacg tccaggcgac caatttcaga acgcagacgg
     5521 tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc
     5581 aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagaatcgac gatcaggtct
     5641 ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg
     5701 agcattttca ccgtttcaat gaacggtatc ttgaaatgca gacccggctc ataaaccaga
     5761 ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc gcgctcacct
     5821 tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat aatcgcgata
     5881 actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc gcgttggcgc
     5941 ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct ccactggttg
     6001 tggaggaaga ggctggcggc agacgcagca gattgctggc accgttatcg ctcttcgccg
     6061 caggggcgtt accgcctttc agcatctggt ctaacggcag aaccatcagg ttgccacctt
     6121 tatcgttaac cagcactttg cgggtgttac ccaacacttt ttccatcgtc tcgatataca
     6181 gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta gcaaagcgcg
     6241 ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc tcttcgagga
     6301 tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct tctgcttcac
     6361 gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt
     6421 cttccggcgg acgagctgcc tggaagttga cgtccagcag agtgataccc atgtcatacg
     6481 gacggatagt ctcttccagt tcgcgctgag tatcgctacg aatcacggta cgaccttccg
     6541 tcagaatgcg gtccatggtg tatttaccga taactccacg cagggcgctg tcggtagcct
     6601 gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga ttggtgacgc
     6661 ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc atcacaccag
     6721 aagcggccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtcg ataaacgtcg
     6781 gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt gttaccacgc
     6841 cgcgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg acaatcgctg
     6901 ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc
     6961 caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg ctcagtttgc
     7021 ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt ccatttccct
     7081 cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg
     7141 gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct gttgtctttc
     7201 gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag gtaatcgatc
     7261 aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg aacttgcaga
     7321 cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg ataaaaacga
     7381 cttctcagac gtccttcctg cggcggcaaa cgcaatgtat gctgcgccac ctcgccggaa
     7441 agccgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg tgcggaaagc
     7501 cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa atcttccagc
     7561 atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat ctcttcaaga
     7621 accgtattca ccgcttcgat gttttcttgt acacgtacat ccgccgcatc aatgacgtgc
     7681 agcagtaatg tggcttgccg cgtctcttgt aacgtggctt taaatgctgc caccagatcg
     7741 tgcggcaggt ggcgaataaa ccctacggta tctgcaagta cggtttcacc gacatccgcg
     7801 acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc
     7861 cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata tcccaccagc
     7921 gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg ctgcttttca
     7981 actctttcta ggcgcgactg tatctgcacg atgcgattac gcaacaaacg acggtcggtt
     8041 tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct ttcaaggtgg
     8101 gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgctagctc aacctgcaac
     8161 ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg
     8221 ataacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct cagggcatgg
     8281 tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt ccgcaatttc aactgcttta
     8341 ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat cacctgcaat
     8401 gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc catatctttg
     8461 tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata acggtcaaac
     8521 aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa
     8581 aaaaaacagc ccgaaacctt attcggtttc ttcgctgtcc tgttgcgcgg aagtattctg
     8641 cgcgctgcta ccatgatggt agttactgct ggtaccgcca ccggcgttgt tactgtgatg
     8701 agaaaccggg cgagacggga caacagtaga aatcgcgtgc ttgtaaacca tctggctgac
     8761 cgtgtttttc aacaggatca cgaactgatc aaaagactcg atttgccctt gcagcttaat
     8821 accattcacc aaataaatag aaactggaac acgttcccga cgcagtgcgt tcaggaacgg
     8881 atcttgtaaa gattgcccct tagccattct ctcttttcct tatatgctta tttgtacttt
     8941 gaacctttcg attctgaaaa aattgcgcac gatacgtctc aattgtacac attcagcctg
     9001 cgatagcacc aacaacctgt aatacttcgt cacgcgcctg ttctggtttt tcactgtcaa
     9061 gccagtgaac cccttcccaa ccacgcagcc aggttatctg ccgcttcgcc aactgtctcg
     9121 tggcgcaaac acctcgataa accatttcat cgtatgagat ttcgccttca aggtaagacc
     9181 acatctggcg ataacccacg caacgaatgg aaggcaagtc cgtatgcaaa tctcctcggg
     9241 caaaaagcgc ccggacttct gcttcaaaac ctgaagccaa catctgatga aaacgctgct
     9301 caatgcgttg atggagcagt tcacggctcg ccggggcgat ggcgaactga tgcacctgat
     9361 acggtagagc gtctcctgac gtttgcgtca gttccgttaa agttttaccc gaaatgaaaa
     9421 aaacttccag tgcccgggaa agcctttgtg gatcatttgg atgaatcctt gctgccgcaa
     9481 ccggatctac ctcctgaagt tgacgatgca atgactccca accttgctct gccgcctgtt
     9541 gctcaattct ggcccgcact tccgggtctg ccgacggtag cggcgacaac ccttccagca
     9601 atgccttgaa atacaacatc gtaccgccca ctaacagtgg gatccgcccc gccgcggtga
     9661 tatcggccat ttccgccagc gcatcgcggc gaaaatcagc agccgagtaa gcctgcgacg
     9721 gatcgcgaat atccagcaat cggtgcggcg cggcgagtaa ctcttcagcg ttcggcttcg
     9781 ccgtcccgat atccatccct ttgtaaataa gggcagaatc aacgcttatc aactctactg
     9841 gtaaaatttt acgcagctca atggctaacg ccgttttacc ggaggccgtc ggccccatca
     9901 aaaaaatcgc cttaggcagg ctcgccttac tgatatcact catctttcag ggcttttatc
     9961 gccggatgta aatcaacaga ttgtaacaga ccacccggcg gcgttttcac aagttgcgga
    10021 cataaccgtt ccacgtccgc cagcagggtt atggcctgtg ccattgacca ctgcgcatgt
    10081 tcgctcatca gatttcgtgc aatccactgc gcaatattgc caggttcgaa tacggactgc
    10141 ttcgccaggt agcctatcag ttcaggaatc aagatttgta aattttgttg gcgtaagggt
    10201 aaaggcacgg ccctgatggt cacatgctgt gcatctgact ggaaatcaat acccaattcc
    10261 gccagggcag actgcgcttt ttctaatgcc gatttttctt cgccagaaac ttttagccgc
    10321 aacggaataa gcagcggctg agcgcaaact ggcgcttcac ccggcgtcag ttgtacctga
    10381 cgcagccaac gttctgccac tggcaaggct aaaagtgaaa tgttgccgtc gcgctccagc
    10441 aacgcacagt cggaatggac gatagtcagt acccgaccaa aactctgact gttcgccgca
    10501 agtgcaggtt cctgcggttc cggcgctttt aatttttgca tcggcgcggg cgtttgcaaa
    10561 agctggcgat acacttcacc ttgctgtttc tggtagcctg gctgcgcatt cggccagggg
    10621 gcagccggac gactgcctga tgctggcgca ggagtgtagc gcggagctac cggctcacga
    10681 gctgccggtt ctgcaaagtg attgcgcccc gccgccacgc ggttttccgg aatggaacgc
    10741 ggtgcaggtt ggggttcatc gtccagcggt agcggcgttt ccagttgctg ttgtagcacg
    10801 ctcagcacgc cctgatagat aaaatcatgc accagacgcg actgatggaa acgcacttcg
    10861 tgtttggcgg ggtgcacgtt gacgtccacc tgatgcggat cgatctccag atacagcaca
    10921 aatgccggtt gctgatcggc ccccagtttg tcttcgcagg cctggcggat cgcgtgattg
    10981 atcaggcgat cgcgcatcat gcgaccgttc acgtagcaat actgaatttc tgccagtgcg
    11041 ggcgtggtgt gatttggatc ggccacccag ccgcgtagcg tgagatcgcc gtgttgccat
    11101 tcaatcgcca gcgcttgttc aagaaaagcg gtgccgcaaa tcgcgcctaa gcgccgttct
    11161 ttttgcccgc cttccggcac tgcgcggtac tgacgcacaa ttttaccgtt atgcgacagg
    11221 ttgatcgtga cgtcgaaacg cgccagcgca atgcggcgga tgatctcatc aatgtggtta
    11281 aattcggttt tctcggtgcg caggaatttg cgccgcgccg gggtgttgta gaacagatcc
    11341 agcacctcca gcgtcgtccc cacaggatgc gccgccggtt ttaccgtcac gttcatatcg
    11401 cgcccttcgg cataggcctg ccaggcttcc tgctgttctg cggtgcgtga agtgagcgtc
    11461 aggcgggaaa ccgaactgat actcgccagc gcctcaccgc gaaagcccag gctgataatg
    11521 gcttcgagat cgtccagaga ggcgatttta ctggtggcat gacgagccag cgccagcgcc
    11581 agctcatctt ttttgatacc gcagccgtta tcacgaatgc ggataagttt cgccccaccg
    11641 cgttcgatat caatatcgat acgcgtcgca cctgcatcga ggctgttttc caccagttct
    11701 ttgactaccg acgcaggtcg ctcgaccacc tcacctgcgg caatctggtt cgccagttgt
    11761 ggcggtaaga cctgaattgg catcaatcgt ccttagtttg gcagcgtgcg atctggcgtc
    11821 gtcaccgtac tggcagtttg tgccgttgcc ccctgcggcg cagattgcat cggatgcgca
    11881 aggaaataat tgcgcaggcc tttgtaaatg gcttctgcca gctgttgttg gtaatcgtcg
    11941 ctcgccagca aacgttcttc gctgttgttg ctgataaaac cggtttcgac cagtactgac
    12001 gggatatccg gcgaacgcag aacgccgaga ctcgcgtgtt ctggtcggcg tttatgtatt
    12061 tcgccaatgc gttgcaactg actgatcata ctggtcgcta catcataccc tacccgctgg
    12121 gaatgaccga actgtaaatc cagcaccgcc tggcttaaat aggggtcaga ctgactgttc
    12181 gccagcacat cgcccgcccc acccagcagc tccgactgtt tctcgtgctg ctccagccag
    12241 ctggccattt cgctgttggc gcgacggtta gagagcaccc atacggaagc gccagtcgca
    12301 ctgcggttcg gtgcggcatc agcgtgaatc gacacgagga aattggcgtt ttgcttacgt
    12361 gccacatcgc tgcgccccat caccgagata aagtaatccc cgtcacgggt taaaacgcct
    12421 ttaaacatcg gatcgtcatt gagcaaagtg cgcaatttac gcgcgatggc gatggtgaca
    12481 tttttctccc gcgtaccacc ggggccgata gcgccagggt cctgaccgcc gtgtccggca
    12541 tcaatagcga tgataatttt atcgccagtg ttagccgtcg cgcgcgctgc cggacgcgtt
    12601 accgtattac tgctgataac acccgtagtg cggttacttt ccgttttaaa cggattgcgc
    12661 gccggttcgc tgacgcgcgg tgcgacaacc gcaggcgttt caacgcgttt cgcaaccacg
    12721 ggcggtggag gaggcggtgg cggcacatcg gcgttaatcg taaagacgac agtgtaattg
    12781 ctgccattct gccgcttcac cgcttcggtt ttaccgtttt cggtaagatc gaccaccagc
    12841 cgtagcgttt gtgcatcttt aggcgttcca gagcgaatcg ccttcaccag attattgccg
    12901 ctgaacaaca acggcagtcc ctgaatcacg cccgtttgtt tgatatcgag cgccacggtg
    12961 cgtttgcttt gatggctaaa cgcataatca ggatcgccaa taaaactcaa cgttatccgc
    13021 gcctgttgat taccgttaga aacctgaata tcagagagcg tcgcggcacc caccggcgtg
    13081 cacagcagca gcagcgtcgc taccaaccaa tttctgatgc gatacatcat cccgccacct
    13141 ttcaaaggtt aaccggctaa acgcgccagc aacaattcac ccgcagagga aaccgcactc
    13201 acgcgcgcct cacggccttg tgcctgataa tcaatgtgta tttcgacatc cgggtcagga
    13261 agaacacctg taccttgttg tggccactcc accaggcaga tggcatcgtt ggcaaaataa
    13321 tcgcggatcc ccataaactc cagctcctcg ggatcggcaa ggcggtacaa atcaaagtga
    13381 taaaccatta aattgtcgag cgtatagggt tcgaccagcg tataagtggg gcttttgaca
    13441 ttaccctgat gacccagagc ttgtaaaaaa ccccggctaa aggtggtttt acctgcgcct
    13501 aaatcgccat acagatagat tacggttgcg ccatcgcagg ctttcgctac ccgctcgccc
    13561 aggtctaatg ttgcctgctc atcagggagc ggaattactc gattcatcat ggtttttatc
    13621 agtcacttcc gggttaacaa tacgctgtag cgtggaaaag agatcggttg ccagcatccc
    13681 gcgcgttcca aaacgcgccg ccagtacgtc agctgccgca ccgtgcgcga cacagcccgc
    13741 acaggcggca tcatacggcg acagtttttg cccaagcaat gcgccaataa taccagagag
    13801 cacatcgccc atgccgccgc tcgccatgcc tgcatttccg gcatcaataa tgcctaaagc
    13861 gtcaggatgg gcggcgacca cggttccggc acctttcagc accgctacgc cgccataacg
    13921 ttgtaccaga cgtttggcgc aatgtaagcg gtcactttca atttcagcga cggaacagcc
    13981 taacaaccgt gcggcctcgc caggatgcgg cgtgatcacg cgattgtgac gcttatcggg
    14041 attgattgcc agcaggttca atgcatcggc atcccacaac atcggtttgc gaaaattctc
    14101 aactttttgc agtgcttttt tcccccactc ttgctggccc agaccgggac caatcaccac
    14161 cacatcggcc cattccaggc tttcggtaag agagtccatc gtcagttcat gcaccatcaa
    14221 ttccggtcgt gcagtcagca gcggcgcaat gttttcactg cgggtcagta ctcggactaa
    14281 accagcacca gcacgcagcg ccgcttcccc cgtcatacga atagcccccg ccgtgccgtg
    14341 atcgccaccg ataatcacca gccgcccgtg atcgccttta tgcgaagtcg ggcgacgcgg
    14401 tttcagccag tgagaaagtt gttctgccga aaaccgctga attttcgtct cctgacctgc
    14461 cagccagcta tccagcccca gtgagtcaaa atgcagttgt cctgtaacat cccgcgcttt
    14521 tccagtaagc aagcctggtt tcagcgcaat aaaagtgatg gtgtgatcgg cgttgatcac
    14581 tgcgcctggc gtagcgccgg tttcagccag caggccggaa gggatatcaa ccgccacaat
    14641 cggcgcagga tgggaattag cgtggtcgat taactggcta atggattcgc ggggcgcttg
    14701 ctgcaagccg gtaccgagca gcgcatcaac aatcagatct accgattcgg gccagacaat
    14761 attcgaagca tggatctcgc cacccgcgtt taaccatgct tcgcgtgcca gcgcggcctc
    14821 ttccggcaac ggtttgtcgc tctcctgggc caacaacgtg acctcaatgc cgaccgcttt
    14881 ggccagtcgc gcgaccacgt agccatcgcc gccgttatta ccatgaccgc acagcaccag
    14941 ccagtggcgg gcgtcaggat acgccgaacg acacacctgg aatgcggcct cgccagcgcg
    15001 aagcatcagc tcatagagtg tgagccccag cacatctgcc gcctcgcgtt ctccgcggcg
    15061 gatatcgtcg gcgtaccaga cggtgtgtgg tatacttacg gggtttttct tcattgtatg
    15121 gtccgtcatg tcagagcccc tcgatctcaa tcagttagcg caaaaaatta aacagtgggg
    15181 gctggaactg ggctttcagc aggtaggtat taccgatacc gatctcagcg agtccgagcc
    15241 caaactgcaa gcatggctgg acaaacaata ccacggcgaa atggactgga tggcacgtca
    15301 cggtatgctg cgcgctcgcc cccatgagtt attaccaggt acgctgcgtg taatcagcgt
    15361 gcggatgaat taccttcctg ctaacgccgc atttgccagc acgctgaaaa accccaaact
    15421 cggctatgtt agccgttatg cgctgggccg tgactatcac aaacttctgc gcaaccgact
    15481 caaaaagctg ggcgagatga ttcagcaaca ttgtgtttcg ctgaatttta gaccgtttgt
    15541 cgattctgcg cctattctcg agcgcccgtt agctgaaaaa gctgggctcg gctggacagg
    15601 taagcactca cttatcctca atcgcgaggc cggttctttc ttctttttag gcgaattgct
    15661 ggtcgatatt ccgctgcccg tggatcaacc agtcgaggaa ggatgcggta aatgcatagc
    15721 ctgtatgacg atttgcccga ccggtgccat cgtcgagcca tataccgtcg atgctcgccg
    15781 ctgtatctct tatctcacca tcgaacttga aggggcgatc ccggaagagt tgcgaccgtt
    15841 aatgggaaac cgtatttacg gttgcgatga ctgccagctt atctgcccgt ggaatcgcta
    15901 ttcgcaactc actacagaag acgatttcag cccgcgtaag ccgctacacg cacaggaact
    15961 cattgagtta tttgcctgga gcgaagagaa gtttttaaaa gtcacggaag gatcggcgat
    16021 tcgccgtata ggtcacctgc gctggctgcg taatatcgcc gtggcattag gcaatgcgcc
    16081 ctgggatgaa acgattctgg cggcgctgga gagtcgcaaa ggtgagcacc cacttcttga
    16141 tgagcacata gcgtgggcga ttgcgcagca aatcgagaga cgaaatgcgt gcatagtcga
    16201 agtgcaattg ccgaaaaaac agcgtctggt tcgggtgatt gaaaaagggt taccgcgtga
    16261 cgcctgattc atccacagct tgtgaataaa aataaaaacg cattgcaatt caagacgcat
    16321 aaaatcgtca agtgatcgac tcgacaaaac ggaatcttga tttttaactt atttatcaat
    16381 aggttaaaaa aataccattc accgtgcgaa gtttctttga aattaagatt acagcctgag
    16441 gatgtggata actctgttta caagagtttg tcagagacaa caaaaaccag caataacgca
    16501 tcgcgtcgct gtggatattt tattgagaga agaatttgga gcgggaaacg agactcgaac
    16561 tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc gcttgggtgg
    16621 tctgtgcctt acagca
//
LOCUS       DAJTXQ010000092        16583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000092 DAJTXQ010000000
VERSION     DAJTXQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.092"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO515_002849"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO515_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6576732.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO515_002850"
     CDS             2219..2767
                     /locus_tag="OO515_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6576733.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO515_002851"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO515_002851"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6576734.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO515_002852"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO515_002852"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6576735.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO515_002853"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO515_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6576736.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO515_002854"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO515_002854"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6576737.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO515_002855"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO515_002855"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6576738.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OO515_002856"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OO515_002856"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6576739.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO515_002857"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO515_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6576740.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO515_002858"
     CDS             complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO515_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6576741.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO515_002859"
                     /pseudo
     CDS             complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO515_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO515_002860"
     CDS             complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO515_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6576742.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16250..>16583)
                     /locus_tag="OO515_002861"
     CDS             complement(16250..>16583)
                     /locus_tag="OO515_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6576743.1"
                     /translation="SGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVL
                     SAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTRLDTALETFWQALHRRDVQVPDLL
                     SLRGNVHA"
BASE COUNT         4131 a         4325 c         4235 g         3892 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccagatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctcca cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagtga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcga aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggaggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 caatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    13201 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    13501 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    13681 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    13741 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    13861 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    13981 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    14041 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    14101 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    14161 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    14221 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    14281 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    14341 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    14401 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    14461 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    14521 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    14581 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    14641 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    14701 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    14761 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    14821 gctccccgca catgaatatg tttaccgttg aaatcgacgc tttcccccag caataagcgc
    14881 cccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca
    14941 cgccgtcgcc tgccaactct tgtggatcgc tgcctgtgac cagattaaac aacgcacgac
    15001 catttgagag acggtcaagc gtggcggcct ggcgagcggc aacggtaggt gaggttacgc
    15061 tgggacgcag ggcgacaaga aacttcagcc gctgcgtcac cgggatcatc gacgccgcca
    15121 ccagccacgc atcttcacac gatcgccccg ttgggatcag cacaccggta tagcccagac
    15181 gatccgccgc ttgcgcaatt tgttgcagat agccgtgatc aaccgggcgt gaaccttctt
    15241 ccgttccaag ataatgcccg tcaccatggg tcggtaaaaa ccagaacata ttcagactca
    15301 taattgtttt ccttccaggt gagtgggctg ccagatgcgc tggcgaatat caactttttt
    15361 cgacaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc
    15421 agcgctcacc ggtttgatgg tggtgggagg acgatgatcc agatacgagg caatcaccgg
    15481 tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgctcgcgct ggctgcgggt
    15541 taacgcatcg gcctcactaa aggttgccag tacgccctga ataaaagcgc cgtttttttc
    15601 tgcatagggg cgagctgcca gataaaacga tccagtttga ttgagatcgg tgccgtcttt
    15661 cagcacccgc acgccgccct gtaataacgc agcggagtag tagggatccc agatagccca
    15721 ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatca gctggcgtca gataagtggg
    15781 ctggatgtca gtaaacttaa gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga
    15841 actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg ttttgatcgg
    15901 gctgttttct gccaccagaa tcacttcggc tttgggcttc ggtggctcga cgcccacata
    15961 tatcaaatca gccccggcag cctgggcaaa gattggcgga atatccccgg tactgccgag
    16021 atcaatactg ccaacgttta acgcttccaa catttgcgga cccgcgggga actccaccca
    16081 ggagattttt gattgcggat agcgtttttc cagtaactgg tggctttttg ccaataccat
    16141 accaatactg cctttctgat agcctatacg taacgcttca ggcgaggatt ctgcagcaac
    16201 cgcaaaagta gagacgctaa gcaatcccgc cagcgccagt ttaatgatgt tacgcatgga
    16261 cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc
    16321 agaaagtttc tagcgcggta tcaagacggg tttgcagatt tggcgtgaac tggggtttgt
    16381 gatggtaatc aataacttgt gagtcatcgg caaacacgcc gtgcaggagc tcctgggctt
    16441 tcatcgcact taaaactggc ttaagggcat aatcgaccgc cagcagatga gccaccgtac
    16501 cgcccgtcgc cagcggtagc accactttgc cttgcaaagc gcgctctggc agcaggtcga
    16561 gcagggtttt cagcgcgccg gaa
//
LOCUS       DAJTXQ010000093        16293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000093 DAJTXQ010000000
VERSION     DAJTXQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.093"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..578
                     /locus_tag="OO515_002862"
     CDS             <1..578
                     /locus_tag="OO515_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6576744.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="OO515_002863"
     CDS             581..1744
                     /locus_tag="OO515_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6576745.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="OO515_002864"
     CDS             1828..3453
                     /locus_tag="OO515_002864"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6576746.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OO515_002865"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OO515_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6576747.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OO515_002866"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OO515_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6576748.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="OO515_002867"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="OO515_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6576749.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OO515_002868"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OO515_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6576750.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OO515_002869"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OO515_002869"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6576751.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="OO515_002870"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="OO515_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="OO515_002871"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="OO515_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6576752.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="OO515_002872"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="OO515_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6576753.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="OO515_002873"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="OO515_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6576754.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="OO515_002874"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="OO515_002874"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6576755.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="OO515_002875"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="OO515_002875"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6576756.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="OO515_002876"
     CDS             12037..12588
                     /locus_tag="OO515_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6576757.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="OO515_002877"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="OO515_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="OO515_002878"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="OO515_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6576758.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="OO515_002879"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="OO515_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6576759.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="OO515_002880"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="OO515_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6576760.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="OO515_002881"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="OO515_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6576761.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="OO515_002882"
     CDS             complement(14856..15827)
                     /locus_tag="OO515_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR6576762.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..>16293)
                     /locus_tag="OO515_002883"
     CDS             complement(15880..>16293)
                     /locus_tag="OO515_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6576763.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3825 a         3981 c         4333 g         4154 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc cca
//
LOCUS       DAJTXQ010000094        16076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000094 DAJTXQ010000000
VERSION     DAJTXQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.094"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..566)
                     /locus_tag="OO515_002884"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="OO515_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OO515_002885"
                     /pseudo
     CDS             complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OO515_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OO515_002886"
     CDS             complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OO515_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6576764.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3071..3289
                     /locus_tag="OO515_002887"
     CDS             3071..3289
                     /locus_tag="OO515_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6576765.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3297..4238
                     /gene="ribF"
                     /locus_tag="OO515_002888"
     CDS             3297..4238
                     /gene="ribF"
                     /locus_tag="OO515_002888"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6576766.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4281..7097
                     /gene="ileS"
                     /locus_tag="OO515_002889"
     CDS             4281..7097
                     /gene="ileS"
                     /locus_tag="OO515_002889"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6576767.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7097..7591
                     /gene="lspA"
                     /locus_tag="OO515_002890"
     CDS             7097..7591
                     /gene="lspA"
                     /locus_tag="OO515_002890"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6576768.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7716..8165
                     /gene="fkpB"
                     /locus_tag="OO515_002891"
     CDS             7716..8165
                     /gene="fkpB"
                     /locus_tag="OO515_002891"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6576769.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8167..9117
                     /gene="ispH"
                     /locus_tag="OO515_002892"
                     /gene_synonym="lytB"
     CDS             8167..9117
                     /gene="ispH"
                     /locus_tag="OO515_002892"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6576770.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9183..10097
                     /gene="rihC"
                     /locus_tag="OO515_002893"
     CDS             9183..10097
                     /gene="rihC"
                     /locus_tag="OO515_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6576771.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10264..11085
                     /gene="dapB"
                     /locus_tag="OO515_002894"
     CDS             10264..11085
                     /gene="dapB"
                     /locus_tag="OO515_002894"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6576772.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11541..12689
                     /gene="carA"
                     /locus_tag="OO515_002895"
     CDS             11541..12689
                     /gene="carA"
                     /locus_tag="OO515_002895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6576773.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12707..15928
                     /gene="carB"
                     /locus_tag="OO515_002896"
     CDS             12707..15928
                     /gene="carB"
                     /locus_tag="OO515_002896"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6576774.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3922 a         3783 c         4254 g         4117 t
ORIGIN      
        1 tcatcccccc agtagaaagg aatggagata aacagattaa aacgctcctc ttctttatta
       61 ttttcatcat aagattggct ggcagagaaa gtataactaa tgtgttgcca gttattggag
      121 taactgaatt ggtaatcttt agcatttcca cttcgccccc agtaattccg ccaaagcgca
      181 cttaatgata catttcccag attattggat aaaggttgca taatattggc agaaaggcta
      241 ttttttctgc caatatcata aaaatcatca taatcactct gatgatagtg tttatcattt
      301 tcgtaaagat ggtcgctgaa tgtcctgtaa tcctgcgaag cataacgcca ggcggccacg
      361 ctaaatcgag tggctgtttg tactagatat ttattatagg cgacctgata gctcgtacct
      421 tcgtgtctgg tatcattatt taacttacta ctcgatcggg cagcgtaaaa agagatggca
      481 cccagcggtg tattccaacc atttcccaga gttatagcat tatagttatc ggataaaatc
      541 gtacccgatt atgtgatcag gttgttatta tttagattaa tgtttaaatt ttttaatttc
      601 ttgatgtgaa gttgatgaaa gtttgaaaat ttaatattct taattgttcc cttctttata
      661 agaagagcaa ggtgccatca ttagagtgac agagtaggat gtttcagagc atgcttcaga
      721 ttgtttttta tttctctgca gtgtattaat aatcatcaca gataatttca ttctaaaatt
      781 acctctttct ttgagtgttt taatcctgat gaactatatt ttttataagg agcatgcact
      841 cgttgatgct ctgaaggtat tttctcattt ttgatgatgt tttgtcgtaa tcgtgcataa
      901 acttattaag ttctttatac acatcattat ttagctttac attatcgcga actaaagttt
      961 gtaccaatgg tatcaaaatt tcgaaacggg acggtttgtc atcaatactt gccagggttt
     1021 tgaatactcc tgtgtatctg taacctaagt gcttattgac aagttgagtg gtccatcggc
     1081 caggcataat atcttcacag cgcaaatttt ttatcatggt attagtgaac gatgttccaa
     1141 atatgttgct ttttttaatg aagaggttgt ttatttctcc atttgaaaag atggtgtttg
     1201 ataagtcgct ttcaaaacat gatatattgg ttaatacaca acaaaaataa tacccatatg
     1261 aaaggttgct cttttctatt gttatatttg atagcaacat tttatgtata ttaatattaa
     1321 gagcagtcaa atactggatc ttttgatcgt taagttcata catctccagt gatttattat
     1381 agggtaataa aagtataaat tcctttatat tatctccgca gtaagaaaag acgacattaa
     1441 atttattagt ggccatgttt tcagttgagc agtaagctga tttttctatt aaccgactgg
     1501 gcacgctttt aaataaagat aattttccac atagttttga atatgccgaa ttgctagttt
     1561 taaagttctg ccacattatt ccctggttat catctatcga tgaggagggg attttattgc
     1621 attgctcatt gtatagctca gggaaaatat tttttttaat aagctttttg atttcctggt
     1681 taagatgact ttttttcttg tctattgcac tgtattttgc gttttgtgac gtttcctgat
     1741 gatcataaaa tgactggaga tttctgcagg cctcataaaa tatttcagga gtatatagct
     1801 gtgggtatat ttcttggttg aagtttgcct ttaaaaaatt aacgaatttt tcatggcatt
     1861 cttggtatat cttaatctct tcagaaagac attcgcttgt tctgtcatca aaaacatcca
     1921 atctaagtaa tgtgatacct ttgtgtttaa aaaataaaat tgaactgtat gttctatgag
     1981 tttacatcta taaaaatatg catctctcag atgagtatat tgaaaaaaat gatgaaggac
     2041 tggagatggt ttaggcaagt tatggcttct aaaaaagtca tcaaacaaaa ttaagaactc
     2101 tgttttatac tcgcaaaata cttttactaa ctcattttgt cttgttttat ttattattac
     2161 atcatctact atatttgatt tataaagaca atgtggcgaa gttgagatac catttgtcat
     2221 tatcagtcct tgaagttgat gaatttttat taatttaatt tatgagtgaa tgtgactact
     2281 atcaaaaata gctcattaat tttatgaagt agaatataaa taggtattta acctgtgttg
     2341 attgttgtta attatttaca agatctaata aggagatatt tatgaatggt taaagtgtat
     2401 aggaatttaa ttcaaaaata gagtggtctt aagtttaata cctcaatgtg tatcacaata
     2461 tccacattct ttgtagggga gtctggagat tgagtagata ttcttgttca gaatgtatca
     2521 gccgatggtt ctacgattct taagccacga agagttcaga aagtgtaacg gcatgtctgc
     2581 aaaagccagc atcttttgac tatctggcaa ccggtagtgt gttctctcac gcatcataaa
     2641 agcagcaggc ataaaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
     2701 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
     2761 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
     2821 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
     2881 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
     2941 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
     3001 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
     3061 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
     3121 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt
     3181 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
     3241 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
     3301 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     3361 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     3421 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     3481 tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc
     3541 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt
     3601 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg
     3661 ccgtagggga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     3721 aagctagcat ggaatacggc ttcgatatca ctagtacgca aactttttgc gaaggtggcg
     3781 tgcgtattag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
     3841 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     3901 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     3961 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     4021 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     4081 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     4141 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     4201 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     4261 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     4321 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     4381 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     4441 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     4501 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     4561 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     4621 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     4681 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     4741 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
     4801 gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg
     4861 cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt
     4921 ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
     4981 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     5041 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     5101 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     5161 gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg
     5221 acgttccggc aatcctcggc gatcacgtta ccctagatgc cggtaccggt gccgttcaca
     5281 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     5341 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     5401 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     5461 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     5521 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     5581 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     5641 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     5701 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg
     5761 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga
     5821 aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat
     5881 ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc
     5941 acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc
     6001 taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct
     6061 ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc
     6121 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     6181 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
     6241 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag
     6301 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     6361 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     6421 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     6481 gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc
     6541 acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     6601 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     6661 gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga
     6721 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     6781 gctcgctgga agcggcagta accttgtatg cagaaccgga actgtcggcg aaactgaccg
     6841 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     6901 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     6961 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     7021 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     7081 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     7141 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     7201 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     7261 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     7321 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     7381 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     7441 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     7501 caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg tgctggaagg
     7561 ttttttgcct tctagagcga aaaaacaata ataaaccctg ccggatgcga tgctgacgca
     7621 tcttatccgg cctacagatt gctgcgaaat cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaaaa tcctcaaata taagagcaaa cctgcatgtc tgaatctgta cagagcaata
     7741 gcgccgtcct ggtgcacttc acgctaaaac tcgacgatgg caccaccgcc gagtctaccc
     7801 gcaacaacgg taaaccggcg ctgttccgcc tgggtgatgc ttctctttct gaagggctgg
     7861 agcaacacct gttggggctg aaagtgggcg ataaaaccac cttctcgttg gagccagatg
     7921 cggcgtttgg cgtgccgtca ccggacctga ttcagtactt ttcccgccgt gaatttatgg
     7981 atgcaggcga gccagaaatt ggcgcaatca tgctttttac cgcaatggat ggcagtgaga
     8041 tgcctggcgt gatccgcgaa attaacggcg actccattac cgttgatttc aaccatccgc
     8101 tggccgggca gaccgttcat tttgatattg aagtgctgga aatcgatccg gcattggagg
     8161 cgtaacatgc agatcctgtt ggccaacccg cgtggttttt gtgccggggt agaccgcgct
     8221 atcagcattg ttgaaaacgc gctggccatt tacggcgcac cgatatatgt ccgtcacgaa
     8281 gtggtacata accgctatgt ggtcgatagc ttgcgtgagc gtggggctat ctttattgag
     8341 cagattagcg aagtaccgga cggcgcgatc ctgattttct ccgcacacgg tgtttctcag
     8401 gcggtacgta acgaagcaaa aagccgcgat ttgacggtgt ttgatgccac ctgtccgctg
     8461 gtgaccaaag tgcatatgga agtcgcccgc gccagccgtc gtggcgaaga gtcgattctc
     8521 atcggccacg ccgggcatcc ggaagtggaa ggtacaatgg gccagtacag taacccggaa
     8581 gggggaatgt atctggtcga atcgccggac gatgtgtgga aactgacggt caaaaacgaa
     8641 gagaagctct cctttatgac ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc
     8701 gacgcgctgc gtaaacgctt cccgaaaatt gtcggtccgc gcaaagatga catctgctac
     8761 gccacgacta accgtcagga agcggtacgc gccctggcag aacaggcgga agttgtgttg
     8821 gtggtcggtt cgaaaaactc ctccaactcc aaccgtctgg cggagctggc ccagcgtatg
     8881 ggcaaacgcg cgtttttgat tgacgatgcg aaagatatcc aggaagagtg ggtgaaagag
     8941 gttaaatgcg tcggcgtgac tgcgggcgca tcggctccgg atattctggt gcaaaatgtg
     9001 gtggcacgtt tgcagcagct gggcggtggt gaagccattc cgctggaagg ccgtgaagaa
     9061 aacattgttt tcgaagtgcc gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc
     9121 attagcagcc taagttatgc gaaaatgccg gtcttgttac cggcattttt tatggagaaa
     9181 acatgcgttt acctatcttc ctcgatactg accccggcat tgacgatgcc gtcgccattg
     9241 ccgccgcgat ttttgccccc gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg
     9301 tctcggttga gaaaaccacc cgcaacgccc tgcaactgct gcatttctgg aatgcggaga
     9361 ttccgctcgc ccagggagcc gctgtgccgc tggtacgcgc accgcgtgat gcagcatctg
     9421 tgcacggcga atcgggaatg gctggctacg actttgttga gcacaaccga aagccaatcg
     9481 ggataccagc gtttctggcg attcgtgatg ccctgatgcg tgcaccagag cctgttaccc
     9541 tggtggccat tggcccgtta accaatattg cgctgttact ttcacaatgc ccggaatgta
     9601 aaccgtatat tcgtcgtctg gtgatcatgg gcggttcttc tggtcgcggc aactgtacac
     9661 caaatgccga gtttaatatc gctgccgatc cagaagcggc tgcctgtgtc ttccgtagcg
     9721 gaatcgaaat cgtcatgtgc ggtctggatg tcaccaatca ggcaatatta acccctgact
     9781 atctcgctac tttgccggag ttgaatcgca ccggaaaaat gcttcatgca ctgtttagcc
     9841 actaccgtag cggcagtatg caaagcggtc tgcgaatgca cgatctctgc gccatcgcct
     9901 ggctggtgcg cccggactta ttcaccctca agccgtgttt tgtcgcggta gaaactcaag
     9961 gcgaatttac ctcaggcacg acggtggttg atatcgacgg ttgcctgggc aaaccagcca
    10021 atgtaaaggt ggcattggat ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc
    10081 tggctctggc gtcgtaacct gtcacatgtt atcggcatgc agtcattcat cgactcatgc
    10141 ctttcactga tatccctccc tgtttatcat taatttctaa ttatcagcgt ttttggctgg
    10201 cggcgtagcg atgcgctggt tactctgaaa acggtctatg caaattaaca aaagagaata
    10261 gctatgcatg atgcaaacat ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc
    10321 cagttgattc aggcggcgct ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc
    10381 gaaggatctt ctttactggg cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc
    10441 gttaccgtgc aaagcagcct cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt
    10501 acccgtccgg aaggtacgct gaaccatctc gctttttgtc gccagcatgg caaagggatg
    10561 gtgatcggca ctacggggtt tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc
    10621 gatattgcga ttgtctttgc cgccaatttt agcgttggcg ttaacgtcat gcttaagctg
    10681 ctggagaaag cagccaaagt gatgggtgac tacaccgata tcgaaattat tgaagcacat
    10741 catagacata aagttgatgc gccgtcaggc accgcactgg caatgggaga ggcgatcgcc
    10801 cacgcccttg ataaagatct gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt
    10861 gaacgtgtgc ctggcaccat tggttttgcc accgtgcgtg caggtgacat cgttggtgaa
    10921 cataccgcga tgtttgccga tattggcgag cgtctggaga tcacccataa ggcgtccagc
    10981 cgtatgacat ttgctaacgg cgcggtaaga tcggctttgt ggttgagtgg taaggaaaat
    11041 ggtctttttg atatgcgaga tgtacttaat ctcaataatt tgtaaccaca aaatatttgt
    11101 tatggtgcaa aaataacaca tttaatttat tgattataaa gggctttaat ttttggccct
    11161 tttattttta gtgttatgtt tttaaatatt ctataagtgc caaaaattac atgttttgtc
    11221 ttctattttt gttgttttaa tgtaaatttt gaccatttgg tccacttttt cctgctcgtt
    11281 tttatttcat gcaatcttct tgctgcgcaa gcgttttcca gaacaggtta tatgatcttt
    11341 ttgtcgctta atgcctgtaa aatatgcatg agccacaaaa taatataaaa aataccgcca
    11401 ttaagttgac ttttagcgcc catatctcca gaatgccgcc gtttgccaga aattcgtcgg
    11461 taagcagatt tgcattgatt tacgtcatca ttgtgaatta atatgcaaat aaagtgagtg
    11521 aatattctct ggagggtgtt ttgattaagt cagcgctatt ggttctggaa gacggaaccc
    11581 agtttcacgg tcgggccata ggggcaacag gttcggcggt tggggaagtc gttttcaata
    11641 cttcaatgac cggttatcaa gaaatcctca ctgatccttc ctattctcgt caaatcgtta
    11701 ctcttactta tccccatatt ggcaatgtcg gcactaatga cgccgatgaa gaatcttctc
    11761 aggtacatgc acaaggtctg gtgattcgcg atctgccgct gattgccagc aacttccgta
    11821 ataccgaaga cctctcttct tacctgaagc gccataacat cgtggcgatt gccgatatcg
    11881 atacccgtaa gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg
    11941 caggcgataa cccggatgcg gcgttggcgt tagaaaaagc ccgcgcgttc ccaggtctga
    12001 atggcatgga tctggcaaaa gaagtgacca ccgcagaagc ctatagctgg acacaaggga
    12061 gctggacgtt gaccggcggc ctgccagaag cgaaaaaaga agacgagctg ccgttccacg
    12121 tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg gatagaggct
    12181 gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag
    12241 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg
    12301 ccatccagaa attcctcgaa accgatattc cggtattcgg catctgcctc ggtcatcagc
    12361 tgctggcgct ggcgagcggt gcgaagactg tcaaaatgaa atttggtcac cacggcggca
    12421 accatccggt taaagatgtg gagaaaaacg tggtgatgat caccgcccag aaccacggtt
    12481 ttgcggtgga tgaagcaaca ttacctgcaa acctgcgtgt cacgcataaa tccctgttcg
    12541 acggtacgtt acagggcatt catcgcactg ataaaccggc gttcagcttc cagggtcacc
    12601 cggaagccag ccctgggcca cacgacgcag cgccgttgtt cgaccacttt atcgagttaa
    12661 ttgagcagta ccgtaaaacc gctaagtaat caggagtaaa agagccatgc caaaacgtac
    12721 agatataaaa agtatcctga ttctgggtgc gggcccgatt gttatcggtc aggcgtgtga
    12781 gtttgactac tctggcgcgc aagcatgtaa agccctgcgc gaagagggtt accgcgtcat
    12841 tctggtgaac tccaacccgg cgaccatcat gaccgacccg gaaatggccg atgcgaccta
    12901 catcgagccg attcactggg aagtagtacg caagattatt gaaaaagagc gcccggacgc
    12961 ggtgctgcca acgatgggcg gtcagacggc gctgaactgc gcgctggagc tggagcgtca
    13021 gggcgtgttg gaagagttcg gcgtgactat gattggtgcg accgccgatg cgattgataa
    13081 agcagaagac cgccgtcgtt tcgacgtagc gatgaagaaa attggtctgg aaaccgcgcg
    13141 ttccggtatc gcacacacga tggaagaagc gctggcggtt gccgctgacg tgggcttccc
    13201 gtgcattatt cgcccatcct ttaccatggg cggtagcggc ggcggtatcg cttataaccg
    13261 cgaagagttt gaagaaattt gcgcccgcgg tctggatctc tccccaacca aagagctgct
    13321 gattgatgag tcgctgatcg gctggaaaga gtacgagatg gaagtggtgc gtgataaaaa
    13381 cgacaactgc atcatcgtct gctctatcga aaactttgat gcgatgggca tccataccgg
    13441 tgactccatc actgtcgcgc cagcccaaac gctgaccgac aaagaatatc aaatcatgcg
    13501 taacgcctcg atggcggtgc tgcgtgaaat cggcgttgaa accggtggtt ccaatgtcca
    13561 gtttgcggtg aacccgaaaa acggtcgcct gattgttatc gaaatgaacc cacgcgtgtc
    13621 ccgttcttcg gcgctggcgt cgaaagcgac cggtttcccg attgctaaag tggcggcgaa
    13681 actggcggtg ggttacaccc tcgacgaact gatgaacgac atcactggcg gacgtactcc
    13741 ggcctccttc gagccgtcca tcgactatgt ggttactaaa attcctcgct tcaacttcga
    13801 aaaattcgct ggtgctaacg accgtctgac cactcagatg aaatcggttg gcgaagtgat
    13861 ggcgattggt cgcacgcagc aggaatccct gcaaaaagcg ctgcgcggcc tggaagtcgg
    13921 tgcgactgga ttcgacccga aagtgagcct cgatgacccg gaagcgttaa ccaaaatccg
    13981 tcgcgaactg aaagatgctg gcgcagagcg tatctggtac atcgccgatg ccttccgtgc
    14041 gggcctgtct gtggacggcg tgttcaacct gaccaatatt gaccgctggt tcctggtaca
    14101 gattgaagaa ctggtgcgtc tggaagagaa agtagcggaa gtgggcatca ctggcctgaa
    14161 cgctgaattc ctgcgccagc tgaaacgcaa aggctttgcc gatgcgcgtc tggcaaaact
    14221 ggcgggcgtg cgcgaagcgg aaatccgcaa gctgcgcgac cagtatgacc tgcacccggt
    14281 ttataagcgc gtggatacct gtgcggcaga gttcgccacc gacaccgctt acatgtactc
    14341 cacttatgaa gaagagtgcg aagcgaatcc gtctaccgac cgtgaaaaaa tcatggttct
    14401 cggcggcggc ccgaaccgta tcggtcaggg tatcgaattc gactactgct gtgtacacgc
    14461 ctcgctggcg ctgcgcgaag acggttacga aaccattatg gttaactgta acccggaaac
    14521 cgtctccacc gactacgaca cttccgatcg cctctacttc gagccggtaa ctctggaaga
    14581 tgtgctggaa atcgtgcgta ttgagaagcc gaaaggcgtt atcgttcagt acggcggtca
    14641 gaccccgctg aaactggcgc gcgcgctgga agctgctggc gtaccggtta tcggcaccag
    14701 cccggatgct atcgaccgtg cagaagaccg tgaacgcttc cagcatgcgg ttgagcgtct
    14761 gaaactgaaa caaccggcga acgccaccgt taccgctatt gaaatggcgg tagagaaggc
    14821 gaaagagatt ggctacccac tggtggtacg tccgtcttac gttctcggcg gtcgggcgat
    14881 ggaaatcgtc tatgacgaag ctgacctgcg tcgctacttc cagacggcgg tcagcgtgtc
    14941 taacgatgcg ccagtgttgc tggatcactt ccttgatgac gcggtagaag ttgacgtgga
    15001 tgccatctgc gacggcgaaa tggtgctgat tggcggcatc atggagcaca tcgagcaggc
    15061 gggcgtgcac tccggtgact ccgcatgttc tctgccagcc tacaccttaa gtcaggaaat
    15121 tcaggatgtg atgcgccagc aggtgcagaa actggccttc gaattgcagg tgcgcggtct
    15181 gatgaacgtg cagtttgcgg tgaaaaacaa cgaagtctac ctgattgaag ttaacccgcg
    15241 tgcggcgcgt accgttccgt tcgtctccaa agccaccggc gtaccgctgg caaaagtggc
    15301 ggcgcgtgtg atggctggca aatcgctggc tgagcagggc gtaaccaaag aagttatccc
    15361 gccgtactac tcggtgaaag aagtggtgct gccgttcaat aaattcccgg gtgttgaccc
    15421 gctgttaggg ccagaaatgc gctccaccgg ggaagtgatg ggcgtgggcc gcaccttcgc
    15481 tgaagcgttt gccaaagcgc agctgggcag caactccacc atgaagaaac acggtcgtgc
    15541 gttgctttcc gtgcgcgaag gcgataaaga acgcgtggtg gacctggcgg caaaactgct
    15601 gaaacagggc ttcgagctgg atgcgaccca cggcacggcg attgtgctgg gcgaagcggg
    15661 cattaatccg cgtctggtga acaaggtgca tgaaggccgt ccgcacattc aggaccgcat
    15721 caagaatggc gaatatacct acatcatcaa caccacttca ggccgtcgcg cgattgaaga
    15781 ctcccgcgtg atccgtcgca gtgcgctgca atataaagtg cattatgaca ccaccctgaa
    15841 cggtggtttc gctaccgcga tggcgctgaa tgccgacgcg accgaaaaag taatttcggt
    15901 gcaggaaatg cacgcgcaga tcaaataata gcgtgtcatg gcggatattt ttcatccgct
    15961 aatttgatcg aataagtaat acggttctct gatgaggacc gttttttttg cccattaatt
    16021 aaatcttttg gggaatcgat atttttgatg acataagcag gatttagctc acactt
//
LOCUS       DAJTXQ010000095        15862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000095 DAJTXQ010000000
VERSION     DAJTXQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.095"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1443
                     /locus_tag="OO515_002897"
                     /pseudo
     CDS             1..1443
                     /locus_tag="OO515_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1531..2502)
                     /gene="lpxM"
                     /locus_tag="OO515_002898"
                     /gene_synonym="msbB"
     CDS             complement(1531..2502)
                     /gene="lpxM"
                     /locus_tag="OO515_002898"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6576775.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2622..3944)
                     /gene="mepM"
                     /locus_tag="OO515_002899"
     CDS             complement(2622..3944)
                     /gene="mepM"
                     /locus_tag="OO515_002899"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6576776.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3960..4892)
                     /gene="znuA"
                     /locus_tag="OO515_002900"
     CDS             complement(3960..4892)
                     /gene="znuA"
                     /locus_tag="OO515_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6576777.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4971..5726
                     /gene="znuC"
                     /locus_tag="OO515_002901"
     CDS             4971..5726
                     /gene="znuC"
                     /locus_tag="OO515_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6576778.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5723..6508
                     /gene="znuB"
                     /locus_tag="OO515_002902"
     CDS             5723..6508
                     /gene="znuB"
                     /locus_tag="OO515_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6576779.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6655..7665)
                     /gene="ruvB"
                     /locus_tag="OO515_002903"
     CDS             complement(6655..7665)
                     /gene="ruvB"
                     /locus_tag="OO515_002903"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6576780.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7674..8285)
                     /gene="ruvA"
                     /locus_tag="OO515_002904"
     CDS             complement(7674..8285)
                     /gene="ruvA"
                     /locus_tag="OO515_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6576781.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8424..8489)
                     /gene="yobI"
                     /locus_tag="OO515_002905"
     CDS             complement(8424..8489)
                     /gene="yobI"
                     /locus_tag="OO515_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6576782.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8562..9164
                     /locus_tag="OO515_002906"
     CDS             8562..9164
                     /locus_tag="OO515_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6576783.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9166..9687)
                     /gene="ruvC"
                     /locus_tag="OO515_002907"
     CDS             complement(9166..9687)
                     /gene="ruvC"
                     /locus_tag="OO515_002907"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6576784.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9722..10462)
                     /locus_tag="OO515_002908"
     CDS             complement(9722..10462)
                     /locus_tag="OO515_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6576785.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10491..10943)
                     /gene="nudB"
                     /locus_tag="OO515_002909"
     CDS             complement(10491..10943)
                     /gene="nudB"
                     /locus_tag="OO515_002909"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6576786.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11061..12833)
                     /gene="aspS"
                     /locus_tag="OO515_002910"
     CDS             complement(11061..12833)
                     /gene="aspS"
                     /locus_tag="OO515_002910"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6576787.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13143..13709
                     /locus_tag="OO515_002911"
     CDS             13143..13709
                     /locus_tag="OO515_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6576788.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14064..14312
                     /locus_tag="OO515_002912"
     CDS             14064..14312
                     /locus_tag="OO515_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6576789.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14913..>15862
                     /locus_tag="OO515_002913"
     CDS             14913..>15862
                     /locus_tag="OO515_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6576790.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFE"
BASE COUNT         4031 a         4028 c         3638 g         4165 t
ORIGIN      
        1 actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat atggaaagcg
       61 atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag agtttatcat
      121 gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat ttctgatgcg
      181 cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca cattttgttt
      241 aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg gctcaaagca
      301 cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt aaatggtctg
      361 aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc agaaagcggt
      421 gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga aaatatgcac
      481 agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga ttttgcttca
      541 aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac tgtgatcagc
      601 gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct tcgtttagcc
      661 tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga ttggcacgaa
      721 cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct tgcacaatgt
      781 caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt cgcttttgcc
      841 aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac tggcatagct
      901 ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga attaactttg
      961 ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga taatttcctg
     1021 attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt tcctcatcat
     1081 ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca tcccttacag
     1141 gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt ttttgctatc
     1201 gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga cggtataatc
     1261 gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg agtatgtaat
     1321 tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag tttttattgc
     1381 aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg attaactcaa
     1441 tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt tttatcgagt
     1501 acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat ctttacgctt
     1561 atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc aggtgtattg
     1621 ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg caatcgtatg
     1681 atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca gacggtgcgt
     1741 cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt tcatcaaacg
     1801 accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt cgctgtgctc
     1861 tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta ccgactggat
     1921 gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac gacgcaccgt
     1981 gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg ccattttctg
     2041 cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg gcaccagaaa
     2101 gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt gccagtcaac
     2161 gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg ccatcgcttg
     2221 cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac tacgttctgg
     2281 aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca gtcgtccggc
     2341 aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta aagcgatacc
     2401 cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa aggatttatc
     2461 aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc agtttcggat
     2521 aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac caaataaaaa
     2581 aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa ccgtagctgc
     2641 ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt cagcccttcg
     2701 gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca tacttcatag
     2761 tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg gtcgccacgt
     2821 ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg cgtggtgtag
     2881 ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt ggcaaccacc
     2941 acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa atcaacacct
     3001 ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa gttagacgag
     3061 atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc agtaccgtta
     3121 cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc ttctgaacgc
     3181 aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc tcgagacatt
     3241 aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca ctgcatggct
     3301 ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct ggcaacaaag
     3361 ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg catttcgctg
     3421 gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg acgagacact
     3481 tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag ttgttgaccg
     3541 attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc acccatatca
     3601 ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac atattcatgc
     3661 acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc ttcatcttct
     3721 tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat ttcgttctgt
     3781 tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata gggccgccag
     3841 acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg tggtcgcggt
     3901 aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc acttcctcat
     3961 taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa ttctgaatag
     4021 cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat acgaacggat
     4081 gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc aaaaacgcag
     4141 gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc gccaggttga
     4201 atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt ttcgaaatag
     4261 ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag ctcgttacca
     4321 acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc gtcaagtttg
     4381 gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc tgtagcccgc
     4441 gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg atcttcgtca
     4501 cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat cagtagcggt
     4561 ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt gcttaccggt
     4621 ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc gttctgtaag
     4681 cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc aggaagtaaa
     4741 acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac gggtttaagc
     4801 gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc ggcggataat
     4861 gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt aatgaatatg
     4921 agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac atgacaagtc
     4981 tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc tctgatgtgt
     5041 cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc gcaggtaagt
     5101 cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt atcaagcgca
     5161 acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc acgttgccac
     5221 tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat attttgcctg
     5281 cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag ctctctggtg
     5341 gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa ttattagtgc
     5401 tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat gaccttattg
     5461 accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat ctgcatctgg
     5521 tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt tccggcacac
     5581 cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt ggtgctgaac
     5641 aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga cgaattgttt
     5701 tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt tagccgggat
     5761 catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc gtatgtctta
     5821 tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt tgttgctgga
     5881 cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg gtctggtatg
     5941 gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg cgcacagtgc
     6001 cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg atttgatggc
     6061 ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta ttgcgattgg
     6121 cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt cgatgacgat
     6181 tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat tgttgttgat
     6241 gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt tgattattac
     6301 ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc cggaacagat
     6361 ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa ccttttccgc
     6421 gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt tatttattat
     6481 cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc ggatgcggcg
     6541 cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag acgcgaatag
     6601 cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc cgacttacgg
     6661 catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca tacgcccacg
     6721 cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat cctcaatggt
     6781 ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc caccaaagaa
     6841 cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt cagcatcgac
     6901 attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat cgtgcttcac
     6961 ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg taccgcgagc
     7021 gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa agcgtgcgct
     7081 gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt gcacaatacc
     7141 aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac caatcagggt
     7201 aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa tcatgatatc
     7261 cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg atagacggtg
     7321 gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg ctgccagatc
     7381 gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca tttcattggc
     7441 gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca acagatgatc
     7501 gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg aacgaacctg
     7561 cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg ccccatcttc
     7621 cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt acctcataac
     7681 gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg agcgattttg
     7741 ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc ggcaaccgct
     7801 tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag atcggcggct
     7861 ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac aattaaacgc
     7921 tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc ttcacgttca
     7981 acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc caacttcggg
     8041 ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt attgttaaaa
     8101 ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat cgcttcctga
     8161 cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata gcctacgccg
     8221 cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct gagtctgcct
     8281 atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa aaaatgctgg
     8341 atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac tgaaaaatgg
     8401 cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat tacatattca
     8461 gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata aattttttat
     8521 gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt aattaccctg
     8581 ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta tttggtcaaa
     8641 tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt cataacggtg
     8701 aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg ctatctcgtt
     8761 ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct gggctgacag
     8821 aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa ctttaccaca
     8881 ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt gatatttata
     8941 aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg ttaaatagca
     9001 atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt ccgtgggagc
     9061 gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg attcacgcgg
     9121 agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac gcagtcgccc
     9181 tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga cgtgacaatg
     9241 ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg gcagtttcag
     9301 caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac ctaccaccgt
     9361 ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca ccgccgccac
     9421 aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt tggccataaa
     9481 gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg tcacgcccgc
     9541 atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc atccgctacc
     9601 caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca cgcgcgaacc
     9661 cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt agcaggcccc
     9721 atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact tcctgcacgt
     9781 cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt tctgcatcca
     9841 tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc agacctgccg
     9901 cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg tcaatcgcgc
     9961 cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc ataatggtgt
    10021 cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag gcaacggaac
    10081 catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca gcaacggtac
    10141 ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga ccgtaacctt
    10201 cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt gcaattgcgc
    10261 ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc gcacgcagac
    10321 gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc tcacgaatga
    10381 ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt ctggtgttgg
    10441 cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt tcaggcagcg
    10501 ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag cgccgcggca
    10561 gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg gcgttcgtgc
    10621 gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc atagcgatga
    10681 cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa ggtcagttgt
    10741 tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc ttgcggcgcg
    10801 gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc gtcacgccgc
    10861 tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat cgaaacggga
    10921 cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct catcccattc
    10981 acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca acgcgaatga
    11041 tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg aatgctcagc
    11101 tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca cgctgccgcc
    11161 gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag cagcatggtc
    11221 agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt cagggcgtcg
    11281 agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat accaaacacc
    11341 gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc gtaaccattg
    11401 atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag ttctgcagca
    11461 gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc ttcaccgtcg
    11521 tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc gtcggtcaga
    11581 ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac aattttcttg
    11641 ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc caggatggct
    11701 tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc cagacctttc
    11761 gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt aacgaagtta
    11821 ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg tagagcggca
    11881 acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac agatttcagc
    11941 agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc agaaccataa
    12001 cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac gcccttcact
    12061 tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg cgcggtcatg
    12121 aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg caggtcttcg
    12181 tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat cagcagctgt
    12241 ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac acgagaaggc
    12301 accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt ttcgatgtcg
    12361 aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc gcgggttttc
    12421 aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag acgcgcttct
    12481 tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat gatagtcagc
    12541 gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt tttttcgtca
    12601 cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc agaggccagc
    12661 tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc gcggtcgcgc
    12721 atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc acacagagtc
    12781 acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg catgagatat
    12841 cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt cgcagcgtta
    12901 gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc tgccgcaccg
    12961 taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc tgaaatcaga
    13021 ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac aaaatttgtc
    13081 gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt tcaggagtca
    13141 gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta caagaaggca
    13201 tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc gccgggaagc
    13261 tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt ggctggtctg
    13321 ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct aaagtgttgc
    13381 ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat atcgaaatca
    13441 tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg cgccgccgag
    13501 gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa tccaccgccc
    13561 gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt agtgccgcca
    13621 gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc cgtgtgcgta
    13681 gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat ggtcgcatcc
    13741 taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact ttaactgcgt
    13801 gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt ctttagaata
    13861 atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg taacaaagtg
    13921 tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca tgccccaaaa
    13981 aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa tactgtataa
    14041 aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa gagaaaatga
    14101 ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga cgcatcagta
    14161 aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc ctttctgtta
    14221 cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg aaagatacct
    14281 gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt aacggctgga
    14341 aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg ttcttgcata
    14401 cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg cagtaccagt
    14461 acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca gacggtgaga
    14521 agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg ctaatggaca
    14581 gcctccttaa cacagttttt tatatacatg attcactgat accagtggca ttaatagcta
    14641 ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct cccccaaaaa
    14701 gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag aagttcttca
    14761 atatataaca tatggagtta atatgcttac acaaactata tattcgcctc ctccagaagt
    14821 tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa gaatccaaca
    14881 cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac aatcacagat
    14941 taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat gaaaattatc
    15001 taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga gacatcgctt
    15061 ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg tctgaattag
    15121 gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata agtaaaaata
    15181 atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc agctataaca
    15241 gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg tcccacaatc
    15301 aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc tcaaataatc
    15361 aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta tcatgtaatg
    15421 ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca atagggaatc
    15481 ttcttagtga actcccccct ctacctcaca acattcactc catatgggca atcgacaata
    15541 tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt gacataaatc
    15601 agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca atagatatta
    15661 gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc tcttcgccgg
    15721 actaccacgg cccgcagatt tacttctcca tgagtgacgg acaacagaat acactccatc
    15781 gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct gatgtatcac
    15841 agatatggca tgcttttgaa ca
//
LOCUS       DAJTXQ010000096        15583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000096 DAJTXQ010000000
VERSION     DAJTXQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.096"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2066
                     /locus_tag="OO515_002914"
     CDS             <1..2066
                     /locus_tag="OO515_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6576791.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2082..3116
                     /locus_tag="OO515_002915"
     CDS             2082..3116
                     /locus_tag="OO515_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCR6576792.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3198..3536)
                     /gene="rcnB"
                     /locus_tag="OO515_002916"
     CDS             complement(3198..3536)
                     /gene="rcnB"
                     /locus_tag="OO515_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6576793.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3755..4579)
                     /gene="rcnA"
                     /locus_tag="OO515_002917"
     CDS             complement(3755..4579)
                     /gene="rcnA"
                     /locus_tag="OO515_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6576794.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4700..4972
                     /gene="rcnR"
                     /locus_tag="OO515_002918"
                     /pseudo
     CDS             4700..4972
                     /gene="rcnR"
                     /locus_tag="OO515_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5064..5160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5195..5983
                     /gene="thiM"
                     /locus_tag="OO515_002919"
     CDS             5195..5983
                     /gene="thiM"
                     /locus_tag="OO515_002919"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6576795.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5980..6780
                     /gene="thiD"
                     /locus_tag="OO515_002920"
     CDS             5980..6780
                     /gene="thiD"
                     /locus_tag="OO515_002920"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6576796.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6845..7663
                     /locus_tag="OO515_002921"
     CDS             6845..7663
                     /locus_tag="OO515_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6576797.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7715..8461
                     /locus_tag="OO515_002922"
     CDS             7715..8461
                     /locus_tag="OO515_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6576798.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8435..9400)
                     /locus_tag="OO515_002923"
     CDS             complement(8435..9400)
                     /locus_tag="OO515_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6576799.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9397..10401)
                     /locus_tag="OO515_002924"
     CDS             complement(9397..10401)
                     /locus_tag="OO515_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6576800.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10398..11675)
                     /locus_tag="OO515_002925"
     CDS             complement(10398..11675)
                     /locus_tag="OO515_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6576801.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11932..12984
                     /gene="fbaB"
                     /locus_tag="OO515_002926"
     CDS             11932..12984
                     /gene="fbaB"
                     /locus_tag="OO515_002926"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6576802.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13303..14157
                     /gene="gatY"
                     /locus_tag="OO515_002927"
     CDS             13303..14157
                     /gene="gatY"
                     /locus_tag="OO515_002927"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6576803.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14186..15443
                     /gene="gatZ"
                     /locus_tag="OO515_002928"
                     /pseudo
     CDS             14186..15443
                     /gene="gatZ"
                     /locus_tag="OO515_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15453..>15583
                     /locus_tag="OO515_002929"
     CDS             15453..>15583
                     /locus_tag="OO515_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6576804.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3988 g         3779 t
ORIGIN      
        1 gtggctatgt tccaccggaa aactgggagc ggggtattaa tgcgttttat acctcttatt
       61 atgtgagtca gtattacagt gactataaag cgtcgggtaa tagtaagagt acatatgtac
      121 gttttaacag cgggttaaat ttactggggt ggcaactgca ttctgatgcc agtttcagta
      181 aaacaaataa caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg
      241 cccaacttct cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt
      301 ctgttcgctt cagaggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac
      361 aaaattttac gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac
      421 agaatggttt tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt
      481 tgcagttggc cggtggtgga gcagatcttg atgtcagcgt gaaagaggcg gacggttcgg
      541 taaccaccta tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga
      601 aatatgattt tgcggcgggt cgtagccata ttgaaggggc gagcaaacat agtgattttg
      661 tccaggcggg ttatcagtat ggttttaata acttattgac gttgtatggt ggctcgatgg
      721 ttgcgaataa ttattacgcg ttcactttgg ggactggctg gaatacacgc attggtgcca
      781 tttccgtcga tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc
      841 aaagttatca aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg
      901 cggcctggcg ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca
      961 ataaagataa ttatcgccgt gatgaaaacg atgtctatga cattgccgat tattaccaga
     1021 atgattttgg tcgcaaaaat agcttttccg ccaatatgag ccagtcattg ccagaaggtt
     1081 gggggtctgt gtcattaagt acgttatggc gagattactg ggggcgtagc ggcagtagta
     1141 aggattatca gttgagttat tccaacaacc tgcgacggat aagctatacc ctcgcggcaa
     1201 gccaggctta tgacgagaat catcatgaag agaaacgttt taatattttt atatcgattc
     1261 cctttgattg gggtgatgac gtttcgacgc ctcgtcggca aatatatatg tctaactcaa
     1321 cgacgtttga tgatcagggg tttgcctcaa ataatacggg attatcagga acagtaggga
     1381 gtcgggatca gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga
     1441 cagctggagc gaatttaacc tggaacgcgc cggtggcaac agtgaatggc agttatagtc
     1501 agtcgagtac ttatcgacag gctggagcca gtgtttcagg gggcattgtc gcctggtcgg
     1561 gtggcgttaa tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa
     1621 ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg
     1681 tatacgacgg aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg
     1741 atagcgaagc agaattacgt ggcaaccgga aaattgccgc accttatcgc ggcgcggttg
     1801 tactggttaa ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg
     1861 atgggcaacc attaacgttt ggttatgaag tcaatgatat ccacggtcat aatattggcg
     1921 ttgtcggcca gggaagccag ttatttattc gcaccaatga aataccgcca tcggttaatg
     1981 tagcaattga taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg
     2041 aaagtagaaa ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc
     2101 tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat
     2161 ctgttcagca caatttggtt gttcaggggg attttgcact tactcaaaca caaatagcga
     2221 catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga
     2281 gttcgtcgga tattattgtt gggctttata acgataccat aaaattaaat ttacattttg
     2341 aatggaccaa taaaaacaac atcacgttgt caaataatca gaccagtttc accagtggtt
     2401 attcagttac ggtgacacct gcggccagta atgcaaaagt gaatgtttct gcggggggcg
     2461 gcggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg
     2521 ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg gatgcatgtg
     2581 taaatagcta cagaaatgca ttggcacaaa atgcaggtgt ctattccttt aatctgacat
     2641 tgtcatacaa cccgataacc acaacctgca aaccggacga tttattaatt actttagaca
     2701 gtattcccgt ttcacaatta ccagccacag gtaacaaagc aacaataaat agtaaacaag
     2761 gggatattat tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca tcacggaaaa
     2821 tgcaggtgta tttatcaagt tctgatttgt tagccggcag taacacgatc ctgaaaggtg
     2881 cagaaaataa tggagtcgga tttatgcttg aaagcaatgg ttctccagtc acatttttaa
     2941 atatcactga taacagcaaa ggatacacaa atttaaaaga aattgcggca aagtcaaaac
     3001 ttacagatac aacggtttca attccgataa ctgccagtta ctacgtctac gatacaaaca
     3061 aagttaaatc tggcgcactg gaggcaaccg cattaatcaa cgtgaaatac gactaataac
     3121 aagcaagacg agcaagtggc taataataaa aaataacaag gtgtctgcat ttccccctca
     3181 tgatgagggg gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt
     3241 ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg
     3301 gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa tgttgtactc atcggtgcgg
     3361 tacatctctg ggacggtatc acctggtttg aaatgcttaa agtcagcaat gaaactactt
     3421 aactcatact tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag
     3481 gcggcactgg taaccagcaa aagcacaccc agcaacatct tatttttaat ggtcataaac
     3541 tctccacttt ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg
     3601 acaagcgata ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc
     3661 ctaaaagcat ccttagcgag cagagagtca gcggaaatag ctgatttaag aatcatcctg
     3721 ggctgaagat aacatgcaag cctaaaggat tgagttatcg cattatgccc atgaagccat
     3781 gtacgcccat atacacaccg actaagccaa tcaacagact ggaaaaatag ggggcgcgtt
     3841 tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc
     3901 caacgcctac ggtgacaagc gttaacgcca ggccaatgct gaaactgacg accagtgttg
     3961 cgcccagtgt cagggctttc aactgaatgc atatcaacag cacggtaatt gctgccgggc
     4021 aggggataag gccaccggtt aagccaaata gcaaaatttg ccagttggtg acctctctac
     4081 catcaaagcg tcgtttaatg tcattggcat gggctcgtgc atgggcatcc tgatactcgc
     4141 catgttcgtg atggtgatga tgtccatggt cgtggtgatc ttcgtgatcg tgatgatgat
     4201 gctcataatc atactcgtgc atattctcca gccagttgcg ttcgccgcgc caggtacgcc
     4261 agaacatcca gaacgcggtg ccaataatga tcactgcgga aatcagctgg agccacggtt
     4321 ctgctgattg agcagtaaag cgcttgctga tcaccatccc gccaaaggca attaaccaga
     4381 ccactgcggt atgcgaaata gttgctgcca gtccgagcat caccgcttgt ttaatggtgc
     4441 ctttgatggc gatgataaac gccgccatca tcgtttttga gtgccctggt tccaggccat
     4501 gaagcgcacc aagtaagatg gcgctgggga tgaagaacca ggcgtttcct tgctgaagaa
     4561 gagttgtaaa ttcggtcatg ataataattc ttagtattaa ttcggcaatc tgattctact
     4621 ccccccagta cctgatacta ccccccagta gattaatagt gctatgatat tttcatattc
     4681 ttgttaacca ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg
     4741 tgccagtaag attcagggct aggtcgtggc gctcaagaaa atgctcgacg agccgcatga
     4801 atgcgctgca gttttacaac agattactgc tatccgtggc gcggtaaacg gtctgatgcg
     4861 ggaagtgatt aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg
     4921 tgaagaagat ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa
     4981 cgcgattgta aaactgccgt ttttcctcgt ttacaacgcg tgcgctggac attaccatcc
     5041 tcctctgcga tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga
     5101 gaaatacccg tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg
     5161 tcattgcttc ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgggttcagc
     5221 gcaatctgcg cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac
     5281 caatgatgtg gtgcaaacct ttaccgctaa taccttgctg gcgctcggtg catcgccagc
     5341 gatggttatc gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat
     5401 taacgttggc acactgacgc agccacgcgc tcaggcgatg cgtgctgccg ttgagcaagc
     5461 aaaaagctct caaacaccct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg
     5521 ccgccatttt tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc
     5581 ggaaatcatg gcattagctg gcattgctaa tggtggacgg ggagtggata ccactgacgc
     5641 cgcagctaac gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt
     5701 ggtcactggc gaggtggatt atgttaccga tggccatcgc gccgttggca ttcacggtgg
     5761 cgatccgtta atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc
     5821 ctgctgtgcg ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa
     5881 acaagccgga gagcgcgcag tcgccagaag cgaggggcta ggcagttttg ttccacattt
     5941 ccttgatgcg ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg
     6001 acgattgccg gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc
     6061 tcggcacttg gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt
     6121 ggcgtacagt cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg
     6181 ttcagcgatg tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt
     6241 gaagcggtgg cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt
     6301 atgctggcaa aaagcggcga cccactgctt tcaccttcgg cagttgccac gctacgcagt
     6361 cgattattgc cacaggtttc attaataacg ccaaacttgc ccgaagcagc cgccttgctc
     6421 gacgcgccac acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg
     6481 atgggctgtg gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac
     6541 tggctgttta cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac
     6601 acccacggta ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca
     6661 aactgggctg acaccgtaca ggaggcaaaa agctggcttt catcggcgtt agcccaggcc
     6721 gacacgctgg aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga
     6781 gcaccgtact atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa
     6841 accaatgcag ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg
     6901 gctaatttcc atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc
     6961 agttcagatt acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat
     7021 tcgtattcct gccagttaca ccattcacgg aatagatgtt tcgcgctggc aggagcggat
     7081 cgactggcag cgtgtggcaa aaatgcgcga taacggtatt cgcttacaat ttgcctttat
     7141 taaggcgacg gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaactaag
     7201 ccgcgaaaat ggcttgctgc gcggggcata tcattatttc tccccgtcgg tagcagcacc
     7261 ggttcaggtg cgattatttc tgcaaacggt agatttctca caaggcgatt tccctgcggt
     7321 actggacgta gaagagcgag gaaaattatc ggcaaaagaa ttacgcaaga gggtaagtca
     7381 gtggctaaaa atggtcgaaa aaagtacggg gaaaaagccg attatttact caggagccgt
     7441 tttttatcac acgaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta
     7501 ctatcaacgt cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg
     7561 tggacaggta gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggtgga
     7621 ggagttgcag ggattcgttg atgggattaa agaaacgcct taaataagaa gcagtgatat
     7681 actcccacca gactccctgg caggaaaaag acaaatggaa caagcgcata cccagctgat
     7741 tgcccaactg aacgaacgta ttttagcggc ggataacacg ccgctttata ttaagtttgc
     7801 cgaaacggta aaaaatgccg tgcgcagcgg ggtgctggag catggcaata ttttgcccgg
     7861 tgagcgtgat ttaagtcagt taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca
     7921 ggcgctggaa gaagagggcg tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa
     7981 catcttcgaa tactcgttga aagaagcgcg aggtttttct cagcaggtgg tgttgcgcgg
     8041 gaaaaagccc gatacgctat gggttaacaa gcgtgtcgtg aaatgtcccg aagaagtcgc
     8101 gcagcagctg gcggtcgaag caggaagtga tgtctttttg cttaagcgta ttcgctatgt
     8161 cgatgaagaa gcggtatcga ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt
     8221 tgatgccatc gggatttcac tttatgacta tttccgcagc cagcacattt acccacaacg
     8281 tacgcgttcc cgcgttagcg cccggatgcc ggatgccgag tttcagtcac atattcagtt
     8341 agatagcaaa ataccggtgc tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc
     8401 tattgagtac agcattagcc actgtcgcag cgatctatac gtttttgtgt gcgaggagta
     8461 gttcctcgcg cgttggcgca caatcaccgc cccgatgccc gacaacccac gacgccactg
     8521 cattgcccag taatacggca tccgccagtg gcagaccaga ggccagcccg gcaagtacgc
     8581 cacctgcatg actgtcgccc gccccaatgg tgtctacaac ttgcgttgga aatgccggaa
     8641 tgcagccaga agcgtcgttg ctgaaatacc atgcgccttc tttatcgaga cgaacgatca
     8701 acggcgcggc aaatttctcc tgccattgct cgccaagtgt tgttatctct gcggataaag
     8761 caaaacgttc ggcggcaatc tcagcctctt gacgattgag cgacactaaa ggtcgacagg
     8821 ccatgatccg cgccagtaat gcatccggga tatcgccaat acgtgggcca aaatcgataa
     8881 acggcgtcac gtcttgcagc tcttccagcc attccactaa caattcgccg cagggcgagg
     8941 ccagttgata accggaaaaa tagagcaggc tgccaggcgc aacggttaat cgcgccagcc
     9001 actggcgatt ccactgattt tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg
     9061 gctcaaccag cgccagacac cagccgttat caccttcggc gttatcgatc aggctgatta
     9121 agccctcttt tgccatccgg ttgcgaataa tctccgccca cacgccctga ccgagcggca
     9181 aggcattacc cgcttcgatg ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc
     9241 cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca cccacgccag gggagggcat
     9301 aagcgtcggc gatcacgtca atgaccgccg cgccgacgac catcaccggc tgacgcgtgg
     9361 ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat accgcctccc gttgttgacg
     9421 atatttcgcc agcgctgtgg catagcggtt gaaatcaagc tgatttaccg catccagttc
     9481 cgcctttaac gcaggatcga tagcgttaac gccatgcaac gcgccgcaaa ttgccgtcgc
     9541 catagcaccg atggtgtctg tgtcgccgcc aaggttagcg cacaggacgg cgcagcgatt
     9601 cgggtcggtt tgtgctagtt caaccagcgc aatggcgcac ggaacggact caatagtgct
     9661 ggtacctgcg ccaacgacct ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt
     9721 gcgcacaatt ttcagcgcaa tctccagccg cgccgccagt gaggcgctga acgtagtgat
     9781 gcgtttttgc tgtgcatgtc gcgcaattga aggcagggaa tcaacaatcg ctgaccagct
     9841 ttctccgtca atggcacgag aaatcgccca tgcgatgact accgcgcctg caaccgccag
     9901 atcggattta tgggtcgggc tggacgccag cgctacatca tcaataaagg aatcaacatc
     9961 acgcgccgga agcaaacaac ctaatggcga gacgcgcatc gctgcgccgt tggtcacgcc
    10021 gttgttttcc agttcagcaa cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga
    10081 ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc agcgcccagt cgagaatatt
    10141 acgcccaatc agatccggat cgatcttgcc ttcacgttcc agtaacgcat ccgccagaca
    10201 cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt
    10261 ctcctttggt ccaggaagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg
    10321 tggccaaagc tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc
    10381 gagaatacgt tctgttttca tttaacttcc ccttgtgtca acgcaatatc gcgatcatcg
    10441 accttgatag ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg
    10501 ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg
    10561 tttaccggtt cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg
    10621 agcaaactgc cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata
    10681 tgcacggggg cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt
    10741 acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgtacta
    10801 ccgtaaataa agaagccata gcggatcgca gcggtgacca gaccaagcaa taataccttt
    10861 ttgataccaa agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac
    10921 tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg
    10981 gcaaagatgt aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg
    11041 aggaagtttt tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatatcc
    11101 attttgccgg tgctttttgg tggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga
    11161 gcagaacttc cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc
    11221 agtatttgcg gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc
    11281 acacgaatgc gcgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta
    11341 gtcagcgcaa tggtcggcat ataggttaga gagtaagcca gcagtaacgg aaagaacccg
    11401 gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg cgcctgcgaa catcaatacc
    11461 gccagcactt tttgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac
    11521 aggatcgccg caatggcggt acaggcatac gaccagccaa tttctccggc actaaaaccg
    11581 cttttactta accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca
    11641 aacatcatga acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa
    11701 tgtaaggtac gcgatgacaa taccacttaa caataccttt taaatacctt tgcggtgaaa
    11761 acttgaccgc gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca
    11821 tccaaaagat ggaaaaactc gcccgtaacg accaggatta tgattgcccg caaaaggcgg
    11881 gccaggacaa tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat
    11941 attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgacaatt
    12001 ccttctgacc agctttatct ccccggacat gactacgtag accgcgtaat gattgacaat
    12061 aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct
    12121 ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct
    12181 tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa
    12241 gcgggctgta actgtgtggc gtcaacttac ggcgtgttgg cgtcggtatc gcggcgctat
    12301 gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat
    12361 acctacgatc aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg
    12421 gttggtacga ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct
    12481 gcggcctttg aacgcgcgca cgagctgggc atggtgaccg tgctgtgggc ctatttgcga
    12541 aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct gaccggtcag
    12601 gcaaaccatc tggcggcaac catcggtgca gatatcgtca aacaaaaaat ggcggaaaat
    12661 aacggcggct ataaagcaat taattacggt tacaccgacg atcgtgttta tagcaaattg
    12721 accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg
    12781 gctgggttga taaactccgg cggtgctgcg ggcggtgaaa ctgacctcag tgatgcagtg
    12841 cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc tgattcttgg acgtaaagcg
    12901 ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg ccgtgcagga cgtttatctc
    12961 gatagcaaaa ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg
    13021 ataaggcgtt tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc
    13081 atgcctacat agcactgcca cgtatgtcgg aaaaggcgtt tacaccgcat ccggcataaa
    13141 aacacgcgca ctttgctacg gcttccctat cgggaggccg tttttttgcc tttcactcct
    13201 cgaacagttt tcatattgtc gttttgtgat cgttatctcg acatttaaaa acaaatcatt
    13261 tcattatatt ttgaaatcga aaacaagcaa caggatatga aaatgtacgt ggtatcgaca
    13321 aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac
    13381 aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc
    13441 atcgccggaa cgcctggcac attcacttat gctggtacag aaaatctgtt ggcgctggtc
    13501 agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa
    13561 tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg
    13621 catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc
    13681 tttgatgtca gcgtcgaagc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa
    13741 gtcaatgaag ccgatgtttt ttataccaac cccgctcagg cgcgtgaatt tgccgaggca
    13801 accggaattg attccctggc ggtcgccatc ggcacggctc atgggatgta tgccagcgca
    13861 ccggcgcttg atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg
    13921 ctgcatggcg cgtcagggtt atcgactaag gatattcagc aaaccatcaa actggggata
    13981 tgcaaaatca acgttgcaac ggagctgaaa aatgccttct cgcaggcgtt aaaaaattac
    14041 ctgaccgagc accctgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca
    14101 atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc
    14161 acctgccatt taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga
    14221 acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc
    14281 atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca
    14341 atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc
    14401 cgataaagtc ggatttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa
    14461 ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc
    14521 atatgttcgt gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga
    14581 tcccataccg ttagcaccag aaacggttgc ggaacgagct gctttgcttt gctgcggaaa
    14641 gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg
    14701 ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg
    14761 ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa
    14821 cacgcgtgat tgccatcgtg gtgcagccgg gcgtggaatt tgatcacagc aatattatcc
    14881 attatcagcc gcaggaagcg cagccgctgg cgcaatggat agaaaacacc cgaatggttt
    14941 atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc
    15001 actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg
    15061 cactggcaca aattgagcag gaacttatcg cccctgaaaa tcgcagcggt tgcctggcgg
    15121 taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat cgtacgggtt
    15181 ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc
    15241 cgcatagtcg gattaaaaat agcgtcgaaa cgatgatggt gaatcttgaa ggcgtggaca
    15301 tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg
    15361 aattatcagc aataccgcat cagctgatta tggataaaat ttatgatgtt ttgcgcgcct
    15421 atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa
    15481 tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg ctcgccaaag
    15541 gtgtggttca tgatacctgg ccacaggcat taattgccag aga
//
LOCUS       DAJTXQ010000097        15523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000097 DAJTXQ010000000
VERSION     DAJTXQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.097"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO515_002930"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO515_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6576805.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO515_002931"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO515_002931"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6576806.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO515_002932"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO515_002932"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6576807.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO515_002933"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO515_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO515_002934"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO515_002934"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6576808.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO515_002935"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO515_002935"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6576809.1"
                     /translation="MSQPITRENCDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OO515_002936"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OO515_002936"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6576810.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OO515_002937"
     CDS             8229..8939
                     /locus_tag="OO515_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6576811.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTPLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OO515_002938"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OO515_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6576812.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OO515_002939"
     CDS             9636..10343
                     /locus_tag="OO515_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6576813.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OO515_002940"
     CDS             10343..10891
                     /locus_tag="OO515_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6576814.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OO515_002941"
     CDS             10901..12067
                     /locus_tag="OO515_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6576815.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OO515_002942"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OO515_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OO515_002943"
     CDS             13576..14229
                     /locus_tag="OO515_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6576816.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..>15523
                     /gene="ynjE"
                     /locus_tag="OO515_002944"
     CDS             14296..>15523
                     /gene="ynjE"
                     /locus_tag="OO515_002944"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6576817.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVY"
BASE COUNT         3561 a         4220 c         4060 g         3682 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 acagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgcccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atg
//
LOCUS       DAJTXQ010000098        15294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000098 DAJTXQ010000000
VERSION     DAJTXQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.098"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            171..341
                     /locus_tag="OO515_002945"
     CDS             171..341
                     /locus_tag="OO515_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6576818.1"
                     /translation="MKWEGDPPPFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDK
                     YRDGRGREWKDI"
     gene            557..646
                     /locus_tag="OO515_002946"
                     /pseudo
     CDS             557..646
                     /locus_tag="OO515_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(647..1705)
                     /gene="modC"
                     /locus_tag="OO515_002947"
     CDS             complement(647..1705)
                     /gene="modC"
                     /locus_tag="OO515_002947"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6576819.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1708..2397)
                     /gene="modB"
                     /locus_tag="OO515_002948"
     CDS             complement(1708..2397)
                     /gene="modB"
                     /locus_tag="OO515_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6576820.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2397..3170)
                     /gene="modA"
                     /locus_tag="OO515_002949"
     CDS             complement(2397..3170)
                     /gene="modA"
                     /locus_tag="OO515_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6576821.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3336..3485)
                     /gene="acrZ"
                     /locus_tag="OO515_002950"
     CDS             complement(3336..3485)
                     /gene="acrZ"
                     /locus_tag="OO515_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6576822.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3614..4402
                     /gene="modE"
                     /locus_tag="OO515_002951"
     CDS             3614..4402
                     /gene="modE"
                     /locus_tag="OO515_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6576823.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4470..5942
                     /gene="modF"
                     /locus_tag="OO515_002952"
     CDS             4470..5942
                     /gene="modF"
                     /locus_tag="OO515_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6576824.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6203..7219
                     /gene="galE"
                     /locus_tag="OO515_002953"
     CDS             6203..7219
                     /gene="galE"
                     /locus_tag="OO515_002953"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6576825.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7229..8275
                     /gene="galT"
                     /locus_tag="OO515_002954"
     CDS             7229..8275
                     /gene="galT"
                     /locus_tag="OO515_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6576826.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8279..9427
                     /gene="galK"
                     /locus_tag="OO515_002955"
     CDS             8279..9427
                     /gene="galK"
                     /locus_tag="OO515_002955"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6576827.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9421..10461
                     /gene="galM"
                     /locus_tag="OO515_002956"
     CDS             9421..10461
                     /gene="galM"
                     /locus_tag="OO515_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6576828.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10664..11416
                     /gene="gpmA"
                     /locus_tag="OO515_002957"
     CDS             10664..11416
                     /gene="gpmA"
                     /locus_tag="OO515_002957"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6576829.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11575..12627)
                     /gene="aroG"
                     /locus_tag="OO515_002958"
     CDS             complement(11575..12627)
                     /gene="aroG"
                     /locus_tag="OO515_002958"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6576830.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            12943..13323
                     /locus_tag="OO515_002959"
     CDS             12943..13323
                     /locus_tag="OO515_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6576831.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13437..14378
                     /gene="zitB"
                     /locus_tag="OO515_002960"
     CDS             13437..14378
                     /gene="zitB"
                     /locus_tag="OO515_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6576832.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14375..15094)
                     /gene="pnuC"
                     /locus_tag="OO515_002961"
     CDS             complement(14375..15094)
                     /gene="pnuC"
                     /locus_tag="OO515_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6576833.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15132..>15294)
                     /gene="nadA"
                     /locus_tag="OO515_002962"
     CDS             complement(15132..>15294)
                     /gene="nadA"
                     /locus_tag="OO515_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6576834.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3881 a         4024 c         3813 g         3576 t
ORIGIN      
        1 gggcaaaaat tcgcatttct ctccccacca cgatttccag attgaattta acacttgccg
       61 atgtactgga taacttgaaa aaaataaacg gtaaaaacga aaagttactc ggagggaaga
      121 cagccaggac aatcgcagca cagttcagga ttgcccgaga cacatccgga ttgaaatggg
      181 aaggtgaccc accgccattt catgaaatcc gaagtttatc cggtcgcctg cactcagcag
      241 aaaaaggcgg tgatttcact caggcgctcc ttggacatcg ttcatccagc atgacggata
      301 aataccgtga cggacgaggg agggaatgga aagacattta aaattaattt cgaatgattt
      361 cgaaacacca cttgtaatta attgattata aatgattaat tcctaatggg catttgcttc
      421 atgaatgctc ctttgcattt agcaactgac tgtaacagct aaaattagtc gcttttggcg
      481 gtgaatgctg aattaaaacg cagaattttg tccgatagtg gtatcggtgt accattcgcg
      541 cagacgaaaa ttcttaaatg acgcggtaaa agcgcgcgcc aacattgtga ttggtgataa
      601 caccaccgac agcattgccc agttcattta tagccacctg atttaatcag gcggttatcg
      661 acacactttt aatttgcgcg tacagccaca ggccaggttt gatcgccagt tcatccctgg
      721 cccacgggct gatacgcgcc cacagcgttt taccgccgac ttccagctcc acttccacct
      781 ggccgttgtc gtcataacta ttaacaactt ttgcccgcaa tacgttacga atgctggttt
      841 gctgcggcgg ttgtaaaacc agagaaacat cggaagcctg aatgcggata cgtagcgcag
      901 cttgcagcgg ttcgtccagc ttattgaccc acaaatgctg atcgcccagc gccagcgcgg
      961 tcatcgcgta atgcggatga tgctcaagta ccgtcacttt cagaatgcta ctttgttgct
     1021 ctttcggcag ccacggattc atcacgctac tgccccacac ttcctccagc gcgccaaagg
     1081 ctttcacctg accgttttcc agtaccatca ctctgtctgc cagatggagg atctcatcca
     1141 gcgaatggct gacatacaac atcggaatgt tgatttcccg tgtcagccgt tgcagataag
     1201 gcaacagttc gcgtttacgc ggaatatcca gtgacgccag cggttcatcc agcaacagca
     1261 attccggtgc tgtcagcaaa gcccgaccaa tcgccacgcg ctgtttttcg cctccggaca
     1321 ggctgcctgg taaacggtca agcaacggtt caatgcctaa aagcgccacc agcttatcga
     1381 actgatcgac catacttttc gacatgccgt agcgcagatt gccacgcact ttgtaatgcg
     1441 ggaacagccg cgcatcctga aaaacatagc caacgcgacg cttttccggc gtcaggcaga
     1501 tacctttttc ggcatcattt agtacccgcc cattgaggac aatccgccct ttttgcgggc
     1561 gcgtcagtcc actgatggcg ttaatcagcg aagtttttcc ggcaccggag acgccaaaga
     1621 tagcagtgat gccattggcg ggcagtgttt cattaatggt caggcaatgg ttgcccaacg
     1681 tctgggaaaa attcagctcc agcatgatta gcgccccgcc cgttcacggc tgattctggc
     1741 cagccattct gaaatcaaca gggagatcat cgccagcgca atagaaataa tgcacagtct
     1801 cgccgcgcca ctttccccgc cgggggtctg gatcagggta tacatggcag agggaatggt
     1861 tcgcgtttcg ccaggaatgt tcgacacaaa ggtgatggtt gcaccaaact caccgagaga
     1921 acgagcaaaa gccagtaccg taccaacaat aattcccggt aaggtcagcg gtaacgtgat
     1981 agtaaagaaa acgcgccagc gcccggcacc tagtgttctt gcggcctgtt cgagtttgac
     2041 atcaacccct tccagcgcca gacgaattgc ccgcaccatc agcggaaacg acatgacggc
     2101 ggcagcgaga accgcgccgc gccagctaaa ggcgaaggta ataccaaacc agtcatacag
     2161 acgttcaccg ataaatccgc gccgtcccat cgaaactaat aataagtaac cgacgaccac
     2221 gggcggtaac accagcggta gatgcagtac gctgtcgagc agagctttgc ccggaaacgt
     2281 gcaacgcacc agtaaccagg caaaaaagat cccaaacggc aggctaaaca gcacagccag
     2341 ggaagaaact ttcaggctta ataaaactgc ctgccattct ggatcggtca gtatcattac
     2401 ttggttgtaa atccgtaacg tttaaagatt tccgctgcct gcggtccctt cagataatca
     2461 taaaatgctt tcactgtcgc attgttatgc ccttccacaa cagcaaccgg atattccact
     2521 tttttatgtg aatcttccgg gaaggtggca accactttta cccctttgct gacaactgcg
     2581 tcagaaccgt aaacaatgcc caacggcgct tcattgcgtt cgaccaacgc cagcgcgcca
     2641 cgaacatctt ccgccggagc cagtttcggg gagagcgtat cccatgcgcc cagtttttgc
     2701 agtgcttctt ttgcataaat gccagcaggg acatgttccg gatcgccaac cgcgaggcga
     2761 ccgccattca gcagcgaagt ccagttagtt gtgctgtcga tgatgaaatc tttctgctcg
     2821 ctggctttcg gtgctacaac gaccaggcta ttgccgagca gtgtctggcg cgaagccgta
     2881 tcgatcgctt ttttatcaac cgcataatcc atccatttct gatcggcaga gataaacaga
     2941 tccgcaggcg ctcccgcttc aatctgacgg gccagagtag atgacgaagc gaaagaagaa
     3001 accacatcca cgcctttttc ttttttatac tgcgtagcaa tgtcctgcat tgcgttagtc
     3061 agtgatgcgg cggcgaacac cgtgattttc ccttcatctg ccagtgtatt gccagcaaca
     3121 gcgaaagaga gtgccgcccc ggcaaacaag ttcaaccatt tacgagccat tggtaacccc
     3181 ttaatgtaac gttatatagg tgacaatgta acggctagct cagaattttc ccagcagtta
     3241 ttgctaacct ttgtgaggta gataagaaaa aatatcggct ggaaaagcag gaagttgaga
     3301 aaaagaaatg cccgactaag cgggcattca gggaatcaat gattttgtcc gggctggtct
     3361 tttttaccaa caccagaaaa gatgttgaat acttcaccaa gaccgtaaat cagacccagg
     3421 atgatggcca tcacgacagg taccatgatt acggcgaata ccagactttt taataactct
     3481 aacatggtca actccagata tagtcatgag actattctaa ccgctaagca cagaaaagca
     3541 ctcccctttt gtgcggtcag ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg
     3601 ccaggacatt gttatgcagg ccgaaatcct tctcaccctt aagctccaac aaaaattatt
     3661 cgccgacccg cgccggattt cgctactaaa acacattgcg ctttccggtt ccattagcca
     3721 gggagcgaaa gatgccggta tcagctataa aagcgcctgg gatgccatta acgagatgaa
     3781 tcagttaagc gagcatattc tggttgagcg cgcaacaggc ggtaaaggtg gcggcggcgc
     3841 agtactgacc cgctatggtc agcgactgat tcagctctat gacttactgg cgcaaatcca
     3901 gcaaaaagcc tttgatgtgt taagtgacga tgacgccctg ccgctgaaca gcctgctggc
     3961 cgcgatctca cgtttttcac tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac
     4021 cgcccgcgat catgatgaca ttcaacagca tgttgatgtc ttactggctg acggaaaaac
     4081 acgcctgaaa gtcgcaatta ccgcacaaag cggcgcacgt ctggggctgg atgaaggcaa
     4141 agaagtgttg atattgctaa aagcgccgtg ggtaggcatt actcaggacg aagcggtcgc
     4201 acaaaacgcc gacaaccaat taccgggtat tattagtcat attgagcgcg gcgcagagca
     4261 gtgcgaagta ttaatggcgc tacccgacgg gcaaacactg tgcgccacag tgccggtaaa
     4321 tgaagcgact tctcttcagc aagggcagaa tgtcacggcc tactttaatg ccgacagcgt
     4381 gattatcgcc acgctgtgct aagcgtgttg acaatttgtt atgaaacacg tatccctgtc
     4441 agtaatcgct gcacaaaatg ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt
     4501 ttcgtcttag cgacacaaaa acgctgcaat tgcctcagct aacgttaaac gcgggtgata
     4561 gttgggcgtt tgtcggttcg aatggaagcg ggaaatcggc cctggcccgc gcgctggcgg
     4621 gggaacttcc gcttttgaaa ggtgaacggc aaagccagtt ttcccacatc actcgtctct
     4681 ccttcgagca attgcaaaaa ctcgtcagcg acgaatggca gcggaataac accgatatgc
     4741 tcggccctgg cgaagatgac accggacgca ctacggctga aatcattcag gatgaagtaa
     4801 aggatgcacc gcgttgcatg caactggcgc agcagttcgg tattaccgcc ctcctcgacc
     4861 gacgctttaa atacctttcc actggcgaga cgcgaaaaac cctgctgtgt caggcgctga
     4921 tgtcggagcc tgacttgttg attcttgatg agtcgttcga tggcctggat gttgcctcac
     4981 gtcagcagct ggctgagcga ctcgcctcgt tacatcagtc cggtattact ctggtactgg
     5041 tgctcaatcg cttcgatgag atcccggagt ttgtccagtt tgctggcgtg ctggcggatt
     5101 gcacgttagc ggaaactggc gctaaagagg aactgctcca acaagcactc gtcgcgcaac
     5161 tggcgcatag tgaacagctt gaaggtgtgc aactgccgga gccggatgaa ccttcagcac
     5221 gtcacgcctt acccgccaac gaaccgcgca ttgtgctgaa caatggcgtg gtttcttata
     5281 acgatcgccc cattcttaat aaccttagct ggcaggtgaa tccaggtgaa cactggcaaa
     5341 ttgtcgggcc aaatggtgca ggaaaatcga cgttattaag cctggttact ggcgatcatc
     5401 cgcaaggtta cagcaacgat ttgacgcttt tcggacgacg tcgcggcagc ggcgaaacca
     5461 tctgggatat caaaaagcat atcggttacg tcagcagtag tttgcatctg gattaccggg
     5521 tcagcactac cgtgcgtaat gtgattcttt ctggctattt tgattcgatt ggcatttatc
     5581 aggccgtttc ggatcgccag caaaaactgg tgcagcagtg gctggatatt ctcggcattg
     5641 ataaacgtac ggctgacgct ccgttccata gtctttcctg gggacagcag cgtctggcgc
     5701 tgatcgtccg cgcactggtg aaacatccga cgttgcttat tctcgatgaa ccactacagg
     5761 ggcttgatcc gctcaatcgc cagcttatcc gccgttttgt tgatgtgctg attagcgaag
     5821 gtgaaacgca attgttattt gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc
     5881 atcgccttga gttcgtgccg gacggtggac tctatcgcta tgtgctgaca aaaatatatt
     5941 gagtcggtag tgctgacctt gccggaggcg gcctaagcgc cctctccggc caacggttcg
     6001 acgcatgcag gcatgaaacc gcttcttttt tcagataaaa agcgcaatca ttcataaacc
     6061 ctctgtttta taatcactta atcgcgcata aaaaacggct aaattcttgt gtaaacgatt
     6121 ccactaattt attccatgtc acacttttcg catctttgtt atgctatggt tatttcatac
     6181 cataagccta atggagcgaa ttatgagagt tctggttacc ggtggtagcg gttacattgg
     6241 aagtcatacc tgcgtgcaat tactgcaaaa cggtcatgat gtcatcattc ttgataacct
     6301 ctgtaacagt aagcgcagcg tactgcctgt tatcgagcgc ttaggcggca aacatccaac
     6361 gtttgttgaa ggcgatattc gtaacgaagc gttgatgacc gagatactgc acgatcacgc
     6421 tatcgacacc gtgatccact tcgccgggct gaaagccgtc ggcgaatcgg tacaaaaacc
     6481 gctggaatat tacgacaaca atgtcaacgg cactctgcgc ctgattagcg ccatgcgcgc
     6541 cgctaacgtc aaaaacttta tttttagctc ctccgccacc gtttatggcg atcagcccaa
     6601 aattccatac gttgaaagct tcccgaccgg cacaccgcaa agcccttatg gcaaaagcaa
     6661 attgatggtg gaacagatcc tcaccgacct gcaaaaagcc cagccggact ggagcattgc
     6721 cctgctgcgc tacttcaacc cggttggcgc gcatccgtcg ggcgatatgg gcgaagatcc
     6781 gcaaggcatt ccgaataacc tgatgccata catcgcccag gttgctgtag gccgtcgcga
     6841 ctcgctggcg atttttggta acgattatcc gaccgaagat ggtactggcg tacgcgatta
     6901 catccacgta atggatctgg cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa
     6961 gccaggcgta cacatctaca acctcggtgc tggcgtaggc agcagcgtgc tggacgtggt
     7021 taatgccttc agcaaagcct gcggcaaacc ggttaactat cattttgcac cgcgtcgcga
     7081 aggcgacctt ccggcctact gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg
     7141 cgtaacgcgc acactcgatg aaatggcgca ggacacctgg cactggcagt cacgccatcc
     7201 acagggatat cccgattaag gaacgaccat gacgcaattt aatcccgttg atcatccaca
     7261 tcgccgctac aacccgctca ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg
     7321 cccctggcag ggggcgcagg aaacgccagc caaacaggtg ttacctgcgc acgatccaga
     7381 ttgcttcctc tgcgcaggta atgtgcgggt gacaggcgat aaaaaccccg attacaccgg
     7441 gacttacgtt ttcactaatg actttgcggc cttgatgtct gacacgccag atgcgccaga
     7501 aagcaacgat cctttgatgc gttgccagag cgcgcgcggt accagccggg tgatctgctt
     7561 ttcaccggat cacagtaaaa cgttgccaga actgagcgtt gcggcattga cggaaatcgt
     7621 caaaacctgg caggagcaaa ccgcagagct gggaaaaacg tacccgtggg tgcaggtctt
     7681 tgaaaacaaa ggtgcggcga tgggctgctc taacccgcat ccgcacggac agatttgggc
     7741 aaatagcttc ctgcctaacg aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc
     7801 cgaacagaaa tcaccaatgc tggtggatta tgttcagcgc gagctggcag acggtagccg
     7861 taccgttgtc gaaaccgaac actggttagc cgttgtgcct tactgggctg cctggccgtt
     7921 cgaaacgcta ctgctgccca aagcccacgt tttgcggatc accgatttga ccgacgccca
     7981 gcgcagcgat ttggcgctgg cgttgaaaaa gctgaccagt cgttatgaca acctcttcca
     8041 gtgctccttc ccctactcta tgggctggca cggcgcacca tttaatggcg aagagaatca
     8101 acactggcag ctgcacgcgc acttttatcc gcctctgctg cgctccgcca ccgtacgtaa
     8161 atttatggtt ggttatgaaa tgctggcaga gacccagcga gacctgaccg cagaacaggc
     8221 agcagagcgt ttgcgcgcag tcagcgatat ccattttcgc gaatccggag tgtaagaaat
     8281 gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca
     8341 caccattcag gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg
     8401 tttcgttctg ccctgcgcga ttgattatca aaccgtgatc agctgtgcac cacgcgatga
     8461 ccgtaaagtt cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcaa
     8521 tgcgcccatt gtcgcgcatg aaaactatca atgggcgaac tacgttcgtg gcgtggtgaa
     8581 acatctgcaa ctgcgtaaca acagcttcgg cggtgtggac atggtgatca gcggcaatgt
     8641 gccgcagggt gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt
     8701 gcagcagctt tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc
     8761 agaaaaccag tttgttggct gtaactgcgg gatcatggat cagctaattt ccgcactcgg
     8821 caagaaagat catgccttgc tgattgactg ccgctcactg gggactaaag cagtttccat
     8881 gccgaaaggt gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc tggttggcag
     8941 cgaatacaac acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc
     9001 tctgcgcgat gtcaccattg aagagttcaa cgctgttgca catgagctgg acccaatcgt
     9061 ggcgaaacgc gtgcgtcata tcctgactga aaacgcccgc accgttgaag ctgccagcgc
     9121 gctggagcaa ggcgatctga aacgtatggg cgagttgatg gcggagtctc atgcctctat
     9181 gcgcgatgat ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc
     9241 agtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt
     9301 cgcgctgatc ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga
     9361 agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca
     9421 gtgctgaacg aaactcccgc actggcaccc gatggtcagc cgtaccgact gttaactttg
     9481 cgtaacaacg cagggatggt agtcacgctg atggactggg gtgcgacttt actttcagct
     9541 cgtattccgc tttccgatgg cagcgtccgc gaggcgctgc tcggctgtgc cagcccggaa
     9601 tgctatcagg atcaggccgc gtttctgggg gcctctattg gtcgttatgc caaccgcatc
     9661 gccaatagcc gttatacctt tgacggtgaa acggtgacgc tttcgccaag tcagggcgtt
     9721 aaccagctgc acggcggccc ggaagggttc gacaaacgtc gctggcagat tgtgaaccag
     9781 aacgatcgtc aggtgctgtt tgccctgagt tcagatgatg gcgatcaggg cttcccgggt
     9841 aatctcggcg cgacggtgca atatcgtctg accgacgata accgtatctc cattacttat
     9901 cgcgccacag ttgataaatc ttgcccggtg aatatgacta atcacgtcta tttcaatctt
     9961 gacggcgagc agtctgacgt gcgcaatcac aagttgcaga ttctggcggg cgaatatctg
    10021 ccggttgatg aaggcggcat tccgcacgac ggcctgaaat ctgtcgccgg aacctctttt
    10081 gatttccgca gcgccaaaat catcgccagt gagtttcttg ccgacgacga tcagcgcaaa
    10141 gtgaaaggtt acgatcacgc attcttgtta caggccaaag gcgatggcaa gaaagtggcg
    10201 gcgcatgtct ggtcagcaga tgaaaaattg cagctgaagg tctacaccac cgctcccgct
    10261 cttcaattct actccggcaa cttcctcggc ggcacaccgt cgcggggaac cgaaccttac
    10321 gccgactggc aaggactggc gttggaaagc gagtttctgc cggacagccc gaaccaccct
    10381 gaatggccgc aaccggactg cttcctgcgt cctggcgaag agtattccag cctgacggaa
    10441 tatcagttta ttgctgagta atgttaagcc ctcgtcatga gggctttatc ttcatattgt
    10501 tcaaatcacc agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg
    10561 cgccactatt ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtca cggcaatata
    10621 atgagaatta ttatcataaa aagataattt gaggagtaag tatatggctg taactaagct
    10681 ggttctggtt cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta
    10741 cgacgtggat ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa
    10801 agaggaaggt tacagctttg actttgctta cacttctgtg ctgaaacgcg ctatccatac
    10861 cctgtggaat gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa
    10921 actgaacgaa cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ccgccgaaaa
    10981 gtatggcgac gagcaggtga aacaatggcg tcgtggtttt gccgtgactc cgccggaact
    11041 gactaaagat gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa
    11101 agagctgccg ctgactgaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa
    11161 tgaaactatt ctaccgcgta tgaagagcgg tgagcgcgtg atcatcgctg cacacggtaa
    11221 ctctttacgt gcgctggtga aatatcttga taacatgagt gaagaagaga ttcttgagct
    11281 gaatatcccg accggcgtgc cgctggtgta tgagttcgac gagaatttca aaccgctgaa
    11341 acgctattat ctgggtaatg ctgacgagat cgcagcgaaa gcagcggcgg ttgctaacca
    11401 gggtaaagcg aagtaaacgt cattcgttta aaaatgagaa agccgactgc aagtgagtcg
    11461 gcttttttgt tgctaacaat ggagcacatt gcctgatgcg acgctgcgcg tcttatcagg
    11521 cctgtggtga ttcatcggat acgccactct gacggcgcat ccgacaatta aaccttaccc
    11581 gcgacgcgct tttactgcat tcgccagttg acgtaacaga gcatcggtat cttcccagcc
    11641 aatgcaggca tcggtgatgc tcttaccgta ggccagcggc tccccgctat cgaggctctg
    11701 attgccttcc accagatggc tttccaccat cacgccaata atggcctttt cgccaccggc
    11761 aatctgctgg caaacgtcag cacaaacatc catctgcttt ttgaattgtt tggacgagtt
    11821 agcatggctg aaatcgatca tcacctgtgc tggcaggcct gctttgttca gcccttcttt
    11881 cacttcagca acgtgcttcg cgctgtagtt aggctcttta ccgccgcgca gaatgatatg
    11941 gcaatcaccg ttaccgctgg tattcacaat cgccgaatgc ccccatttag ttacggacag
    12001 gaagcagtgc ggcgcaccgg cggcattaat ggcatcgata gccactttaa tcgtaccgtc
    12061 tgtgccattt ttaaaaccta ccggacaaga aagaccagac gccagttcgc ggtgcacctg
    12121 cgattccgtg gtacgtgcac caattgcgcc ccagctcatc aggtcagcga gatactgagg
    12181 agtgatcata tcgagaaact cacccgccgc tggcagaccg ctgtcgttaa tatcaagcag
    12241 caatttacgg gctatacgca gaccgtcgtt gatctggaag ctgttatcca tatgcggatc
    12301 gttaatcaac cctttccagc ccaccgtggt acgcggcttt tcaaaataga cgcgcattac
    12361 gatttccagc tcatctttca gctcttcacg cagcgccagc aagcgagtgg catactcttt
    12421 agccgcgaca ggatcatgaa ttgagcacgg gccaatcacc accaacaggc gatcatcatt
    12481 acctttcagg atcttatgga tcgcttttcg ggcatgggca accgtattcg cggcattttc
    12541 agttacgggg aatttttcca gcaatgcgac aggaggaagt aactctttga tttctttgat
    12601 gcgtaaatcg tcgttctgat aattcatgtc tgttccagtg ttgccatact tatcttagtg
    12661 aatgcaatac ttccaatcta tatcttccgt cagaatgtgt aaacggggtt ttacactatg
    12721 aacggattaa tcctggaatt agcaagaaaa acgccagatt gtcgcgaaaa acgagatctc
    12781 tcctacaatt tctaactgta actcctttgt ttgttaatta tttcaagatt ctctgctgcg
    12841 tttcataaac tggctgaaat cttaaaccaa tgccttatat tcacctgcaa atgcactgtt
    12901 ggaagaggtt atccgacata acgaccataa caggagcatc ctatgaaaat gacaaaactg
    12961 gccacattat ttctgactgc cactctaagc cttgccagcg gtgccgcact ggctgccgat
    13021 agcggagcgc aaactaataa cggccaggca aacgccgcag ctgatgcggg ccaggtagcc
    13081 cctgacgccc gtgaaaatgt cgcgccaaat aacgtcgaca ataacggggt aaataccggt
    13141 tctggcggca caatgctgca ttcggatggt tcttcaatga acaatgacgg aatgaccaaa
    13201 gatgaaaagc acaagaacac catgtgcaaa gatggtcgat gtccggatat caataaaaag
    13261 gtacaaatcg gtgatggcat caataatgat gtcgatacca aaaccgacgg taccacgcag
    13321 taaccctgca aaatagccaa taatgggaga gcggagtctc tcccttttaa tttttcactt
    13381 ctaaaagcag tatgatgtca taaagttaag tgagaataat taagggatga ctcgtcatgg
    13441 cgcactcaca ctcacacacg tcctcacacc tgccagaaga taataatgct cgtcgcttgt
    13501 tgtatgcttt cggcgtgact gccgggttta tgctggtaga agtcgttggt ggttttcttt
    13561 ctggttctct ggcattgctg gccgatgcgg gtcatatgtt gaccgatact gccgccctgc
    13621 ttttcgccct actcgccgtg caattttcac gtcgtcctcc caccattcgc cacactttcg
    13681 gttggctaag actgaccacg ctcgcggcgt ttgtgaatgc catcgccctc gtggtaatta
    13741 ccattttgat tgtctgggag gcgatagaac gcttccgcac gccgcgtccg gtcgagggcg
    13801 gcatgatgat ggcaattgcc gtggctgggt tgctggcaaa tatactttct ttctggttac
    13861 ttcatcacgg cagcgaagag aaaaacctca acgtacgagc agcggcattg catgtgctcg
    13921 gggacctgtt gggttcggtt ggggctatta ttgccgcgtt gattattatc tggaccggct
    13981 ggacacctgc tgaccccatt ctctcgatag tggtgtcgct tctggttctg cgtagcgcgt
    14041 ggcgactgtt gaaagatagc gtgaatgaat tacttgaagg tgcaccggta tcgctggata
    14101 tcgctgaact gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat gtgcaccatg
    14161 tacatgtatg gatggtaggc gagaagccgg tgataacgct gcatgtgcag gtgatcccgc
    14221 cacacgatca cgatgccttg ttggatcaga tccaacacta cctgatggat cactatcaga
    14281 ttgagcacgc caccattcag atggaatatc aaccttgtca tgggccagac tgccacctga
    14341 atgaaggggt gtctggccat tcacatcatc accattaatg ggacagcgcg cgtgagcctc
    14401 tttcacgtgc gctgttgatc cacatccgac tgccgttgag cgcaataaag gtcaggatga
    14461 tgtactccag agacatggcg taaacgccct gaagtgcaaa aataacgacg ctaatcacgt
    14521 taataatcac ccacaacagc cagttttcca catacttacg cgtcatcaga atcattgcca
    14581 cgatagataa caccatcatg catgaatccc agaacgggaa agcgtccggt tgcagttcag
    14641 gcatcacaac ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg gtcaaaaatg
    14701 caaacaccgg attgataaat accgtcatca gaccaatcga aacaacgcaa accgccaacc
    14761 agctgagcgc cttcggcaat ggtaaccagc gaattttcaa ctccgcctcg ttctgactgg
    14821 tttgtcgcga ccacgcatac caaccgtaaa tattcgcggc aaagaaaaac acctgtaata
    14881 gcaggctggc atacagctga atctgaaaga aaataatgcc aaacaaggtg acgttgatca
    14941 ggccaaagaa gtagttgctg atcttctcca gactggcaag gccaatacac agcaacccgg
    15001 cgatcgtgcc taccgcttcg atccatgaga gatcataacc gcctgcccct attggtatat
    15061 gtaccaggat attctgcaca ctaaaaaaat ccatctttcc cccaaagcgt aacgttacgc
    15121 cttattattc gttatccacg tagtgtagcc gcaaaatcca gcatacgatt gagcggcacc
    15181 agcgccctct ctcgcagcct ttcatcaaga acctcgtgat tgcttccttc ctgttctaat
    15241 gcctctgcga tggactgaag gccattcatg gccatccacg gacaatgcgc gcag
//
LOCUS       DAJTXQ010000099        15235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000099 DAJTXQ010000000
VERSION     DAJTXQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.099"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..749
                     /locus_tag="OO515_002963"
     CDS             <1..749
                     /locus_tag="OO515_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6576835.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="OO515_002964"
     CDS             complement(790..1722)
                     /locus_tag="OO515_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6576836.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OO515_002965"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OO515_002965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6576837.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OO515_002966"
     CDS             complement(5254..5886)
                     /locus_tag="OO515_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6576838.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OO515_002967"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OO515_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6576839.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OO515_002968"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OO515_002968"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6576840.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OO515_002969"
     CDS             complement(8729..8839)
                     /locus_tag="OO515_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6576841.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OO515_002970"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OO515_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6576842.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OO515_002971"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OO515_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6576843.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OO515_002972"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OO515_002972"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6576844.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OO515_002973"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OO515_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6576845.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3914 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgct
//
LOCUS       DAJTXQ010000100        15204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000100 DAJTXQ010000000
VERSION     DAJTXQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.100"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..370)
                     /locus_tag="OO515_002974"
     CDS             complement(41..370)
                     /locus_tag="OO515_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6576846.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(375..560)
                     /locus_tag="OO515_002975"
     CDS             complement(375..560)
                     /locus_tag="OO515_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6576847.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(557..3196)
                     /locus_tag="OO515_002976"
     CDS             complement(557..3196)
                     /locus_tag="OO515_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6576848.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3404..4393
                     /gene="ldhA"
                     /locus_tag="OO515_002977"
     CDS             3404..4393
                     /gene="ldhA"
                     /locus_tag="OO515_002977"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6576849.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4504..4926
                     /gene="hslJ"
                     /locus_tag="OO515_002978"
     CDS             4504..4926
                     /gene="hslJ"
                     /locus_tag="OO515_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6576850.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4923..5189)
                     /locus_tag="OO515_002979"
     CDS             complement(4923..5189)
                     /locus_tag="OO515_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6576851.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5463..8987
                     /gene="nifJ"
                     /locus_tag="OO515_002980"
     CDS             5463..8987
                     /gene="nifJ"
                     /locus_tag="OO515_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6576852.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9353..10486
                     /gene="ompN"
                     /locus_tag="OO515_002981"
     CDS             9353..10486
                     /gene="ompN"
                     /locus_tag="OO515_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6576853.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10627..11061
                     /gene="uspF"
                     /locus_tag="OO515_002982"
     CDS             10627..11061
                     /gene="uspF"
                     /locus_tag="OO515_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6576854.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11647..12561
                     /gene="sitA"
                     /locus_tag="OO515_002983"
     CDS             11647..12561
                     /gene="sitA"
                     /locus_tag="OO515_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6576855.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12561..13388
                     /gene="sitB"
                     /locus_tag="OO515_002984"
     CDS             12561..13388
                     /gene="sitB"
                     /locus_tag="OO515_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6576856.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13385..14242
                     /gene="sitC"
                     /locus_tag="OO515_002985"
     CDS             13385..14242
                     /gene="sitC"
                     /locus_tag="OO515_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6576857.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14239..15096
                     /gene="sitD"
                     /locus_tag="OO515_002986"
     CDS             14239..15096
                     /gene="sitD"
                     /locus_tag="OO515_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6576858.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3807 a         3829 c         3678 g         3890 t
ORIGIN      
        1 ctttctcctg tagcgttatc ccgatattgc ggttttgcta ttaaaatttt cgcacccatt
       61 taccgtttat cccttgtaca tattcccccg gtttggcccg agctacgagc ttttgcccgg
      121 cgagtttcgc gatatcatct accgacacat tattttgctt tgccagttgt tggtaactcg
      181 ctttacgttc ggcattaatg tcggttacta atttctctgt ttcagcatcc gttttcagcg
      241 caaccagata accataaaat gtttcaccta cccgcccctg agttctggct tcatccagag
      301 tcaatgccat tacattgctg cttaccagcc caacaagaaa cgcacaaaga agtaatgttt
      361 tcttcatcat cgcctcagaa aagatcgcta cgagtttcaa gcagctcttc gacatctttg
      421 tctgccttga tgatgatctc atgctcaatt ttaacgttca tattgatagt gatcggttcc
      481 ttgggcgcag cgacttcaat acgcggcgta cagccaacca gcataaaaga tgacgtcaac
      541 gcagccagta aaattttcat tgtttttcct cacactcttt tccttgcgga cagtcatttc
      601 ccggcagacg tgcgttctgc tccaaccatg cctggagatt atcgccaaag cgtaaactgc
      661 gccacagcgt aaaaatattc tcttcatgat ggtaattaag gtttaccgta ccgcttttac
      721 catcaacgcg actggtacct gtaatgttgg cctgcatggt taataaaccg agattatcta
      781 agttaatttt tgtcgatgac cggctaattt ccatatagcg caaccagtta attgctgaac
      841 ccgcagtcat attgtctttc accaccgcat ctgcggtgtc tttatccagc cgcaaggtca
      901 ttggcccgct atttgccagc caaccatctt tcactatcca tttttcgttg tttagccata
      961 acggcaatgc accactaaat gccccggaca tggcgaattg tttcggattg acggcgctaa
     1021 ccagttcgct ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt tgcggcatac
     1081 gtaattgttt catcagtacg ttaccgccta acacatcgac gctaacatca gtcaacagca
     1141 agggttcttc ttcagtccac ggataacgcc cttgcaaatc agccgtaata tttttcgctg
     1201 tcaccagatt aatcacttcg gcaattcgca acgtaacggg gccgcgagtc cccagatgcc
     1261 aggctccatc ggcaaaacgg aaaggcagga caaaatcgac accgttaacc tgattatctg
     1321 gcatccaggc actaccgcct ttcaacacgc cgtgtcctcc cgcgcggaat ccttgttcag
     1381 gcgcagcaga aaatgcaacc tgagcataca gttccccatc gcgtaagttc atcttccagt
     1441 cgggtggcac cagcggctgg aatacagtca gtgattgttt tggccaccag gcgttaccgc
     1501 gcagacgaat accgtcccag cgaccattta cccgaaccgg gccaatctca ccagcatgta
     1561 aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc gacgctaaat tttaaggttg
     1621 atggcggtaa cacactgccg ccagtgaaca gcgtttgccc ggcgtccagt gacagcgcgc
     1681 cgctgaagga gggatgctgt gcgtcacgta cccagacgac gggcttgtcg agaattaatc
     1741 gcggcttttc taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg gagaggtcgg
     1801 tcagcgtaat cgtgctgtca tgccattcac cttttcctgc gacatcccag gtggcattta
     1861 tcggtgtaaa actgcctttt ccccagtagc gccactgcca gcggcccgcg tcagggagaa
     1921 aatcattcgc cagcccatcc atatgcagca cgaaatcgcc cagttcatat tcatgcgcct
     1981 gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca cccgctaaag
     2041 gccagcggat ttcatcgatg tccagcgaat cgatgactct tcccttcgaa cgtaataacg
     2101 cgcctggctc aaaggccagc gttgggtcag tcagacttcc acttagctgc gcgggtaagc
     2161 gggcatataa aatgaggtcc gcctgtttcg cttcaccggt cagctgcaga ggcagctggc
     2221 tgttatccat gcttaatttt cctgggccaa aatttagcac cgcgttgcct ttacccgctt
     2281 gcccctgggt cagcacactc agtcgtccgc tgatcagagc gttctcaagc cctgcctgcc
     2341 agttgtcgac tttgacaccc agtcggccac tcaaaggaaa acctgcatac ggccaactcc
     2401 agcgaccatc gcttacggtc aattgttgac gagtaatttg ccacggcaaa tcgagcaacg
     2461 gatcgccgtt atcccgtgcc agcacaatca attgcccgct attttcctgc cagtccagct
     2521 ctgcatccac cagtgacggt tcctgcggca agtttaacgt cgcagtagca tgcccactta
     2581 caggaagtcc atccggcacg agcggcatag taaattcccc caccagttta accggcggct
     2641 gattttcaaa cgcgatgaca tccagttcgc tgactgtaag ttgttgccct ttcagctggc
     2701 cttgaaattt aactttttcg ccctgataac gcagttgctg aatatcagag gttaatgcga
     2761 gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatcg atattgatcc
     2821 aggtgttagg cagcatggac tgccactgcg cgagggtttt tggagcagct ggcgattgct
     2881 ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccatgccg acgttcaata
     2941 accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga caatctccca
     3001 ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg ctttcatcta
     3061 atgcaatacg tgtcccgagc ggtagccaga tgcccgccag cgtgggaacc cacagcccga
     3121 gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg agaacggctt
     3181 tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa gcattcaata
     3241 cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta agcattgttg
     3301 ttgatgaatt tttcaatatc gccatagctt tcaattatat ttgaaatttt gtaaaatatt
     3361 tttagtagct taaatgtgat tcaacatcac tggagaaagt cttatgaaac tcgccgttta
     3421 tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct ttggctttga
     3481 gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca atggctgcga
     3541 agcggtatgt attttcgtaa acgatgacgg cagccgcccg gtgctggaag agctgaaaaa
     3601 gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg accttgacgc
     3661 ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag aggccgttgc
     3721 tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg
     3781 tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt atggcaaaac
     3841 ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt
     3901 tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg aactcggtgt
     3961 ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc tgcactgccc
     4021 gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg
     4081 cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcag caattgaagc
     4141 gctgaaaaat cagaaaattg ggtcgttggg tatggacgtc tatgagaacg aacgcgatct
     4201 attctttgaa gataaatcca acgacgtgat ccaggatgac gtattccgtc gcctgtctgc
     4261 ctgccacaac gtgctgttta ccgggcacca ggcattcctg acagcagaag ctctgaccag
     4321 tatttctcag actacgctgc aaaacttaag caatctggaa aaaggcgaaa cctgcccgaa
     4381 cgaactggtt taatcttgcc gctcccctgc attccagggg agcttattca gataatcccc
     4441 aaagaccttt catcttctat tcttaaaata gccctgagtc agaaactgta attgagaacc
     4501 acaatgaaga aagtagccgc gtttgttgcg ctaagcctgc tgatggcggg atgtgtaagt
     4561 aatgacaaaa ttgctgttac gccagaacag ctacagcatc atcgctttgt gctggaaagc
     4621 gtaaacggta agcccgtgac cagcgataaa aatccgccag aaatcagctt tggtgaaaaa
     4681 atgatgattt ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact gtctaatggt
     4741 gaactgacag ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc gcagcttaat
     4801 gaactcgata acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg
     4861 aaccagttaa cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg
     4921 aattaatagc tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg
     4981 ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg
     5041 ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa acctggcgct
     5101 acgtgcgccg cagtcgcctg ctgaacaggt tcactactac acgccgacaa caataaagcg
     5161 gcacacccta cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga
     5221 caccgccata aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc
     5281 ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta
     5341 cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag
     5401 gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc
     5461 atatgattac tattgatggt aatggcgcgg ttgcttcggt ggcgtttcgc accagtgaag
     5521 ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg
     5581 ccggaaacgg cttgaagaac gtttggggag acacaccatg cgtggttgaa atgcagtcgg
     5641 aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt
     5701 ttacgtcatc gcagggtttg ctgctgatga tcccgacact gtacaaactg gcaggcgaac
     5761 tcacaccgtt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctctctattt
     5821 ttggcgatca ttccgacgtc atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg
     5881 caaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcgacg ctgaaaagcc
     5941 gcgtgccatt tattcatttc tttgatggtt tccgcacgtc ccacgaaatc aacaaaattg
     6001 tcccgctggc cgatgacacg attcttgatc tcatgccgca ggccgaaatt gatgctcatc
     6061 gcgcccgggc gctcaacccg gaacatccgg tgatccgtgg tacgtccgcc aatcctgaca
     6121 cttatttcca gtctcgcgaa gccaccaacc cctggtacaa cgcagtctat gaccatgttg
     6181 aacaggcgat gaatgatttc tctgccgcga caggtcgtca gtatcagccg tttgaatatt
     6241 acgggcatcc gcaagctgaa cgggtgatta tcctgatggg ctcagccatt ggcacctgtg
     6301 aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt cggcgtgctg aaagttcgcc
     6361 tgtaccgccc cttctcagcc aaacacttgc tacaaactct accgggatcc gtacgcagcg
     6421 tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg
     6481 tgatgaccgc actggcagaa gcctttaata atggcaagcg cgaaactctg ccccgtgtca
     6541 ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat
     6601 ttgccgagct caacgcggct aaaccgaaag cgcgctttac ggttggtatt tacgatgatg
     6661 taaccaatct gtccctgccg ttgccggaaa acacccagcc aaactcggcg aaacttgaag
     6721 ccttgtttta tggccttggt agtgatggca gcgtttccgc gaccaaaaac aatatcaaga
     6781 ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag
     6841 cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc
     6901 tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga
     6961 tggctgagcg tttaaaacct ggcggcattt tcctgctcaa cacgccgtac agcgcagatg
     7021 aagtgtggtc gcgcttgccg caagaagttc aggccgtgtt aaaccagaaa aaagcgcgct
     7081 tctatgtgat taacgcggcg aaaatcgccc gcaaatgtgg cctggcggcc cgtattaata
     7141 ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg
     7201 cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggccaggat ctggtggaac
     7261 gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg
     7321 taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga
     7381 aaaccgtaac cgcagcgatg ctcgcagggc ttggtgatgc cctccccgtt tcggcgctgc
     7441 cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag
     7501 agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gcagcttgcc
     7561 cacactcagc tattcgcgca aaagtggtac cacccgaagc gatggaaaac gcccctgcca
     7621 gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg
     7681 tggctccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc
     7741 gtcagaatcc agagattaaa gccatcaata tgatgtcgcg cctggaacat gtcgaagaag
     7801 agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac
     7861 gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattcc ggtgcttgct
     7921 ccggttgtgg cgagacgccg tatattaaat tgctgactca gctctatggc gaccggatgt
     7981 tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt
     8041 ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg
     8101 ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc
     8161 tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg
     8221 ctacgccaga ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg
     8281 ccgaagcaca tgaactgcta cgtgatgcag atgcactggt ggaaaaatca atctggctga
     8341 ttggtggtga tggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt
     8401 tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc
     8461 aggcgtcgaa agcgacaccg ctgggtgcag taactaaatt tggcgagcac ggcaaacgta
     8521 aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga
     8581 tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc
     8641 cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggcac
     8701 tcagccatga ccagatgcgc caactcacag ctaccggctt ctggccgcta tatcgcttcg
     8761 atcctcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag
     8821 aagcactgga agaaacgtta cttcacgagc aacgtttccg tcgtctgaat tcgcagcagc
     8881 cagaagtggc agaacagtta tggaaagatg ctgcagctga tttgcaaaaa cgctatgact
     8941 tcctggcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa
     9001 ggattaccca attctaaaaa aaagcccgga cgactgttcg ggcttgtctt tttatatgtt
     9061 ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca ataaaggcat
     9121 ataacccgcg cagaataacg tatagaaaat taaaagtata atttttattt tgtatgtatt
     9181 atcttcgata cattcaccag acttattatc atttcagaag aattatttga ttaaggtttt
     9241 acttaaggcg taacaaatga tatttatcgg ctaactgaac ttctccttta ggatgttttc
     9301 actccccgga atggggataa aaggcaaata aaataactaa aggatttatt caatgaaaag
     9361 caaagtactg gcacttttaa ttcctgccct gctcgccgca ggtgctgcac atgcagccga
     9421 agtttataat aaagacggca acaaattaga tctgtatggc aaagttgatg gcctgcatta
     9481 tttttctgat aattcagcga aagatggcga tcagagctat gcgcgtctgg gttttaaagg
     9541 cgaaacccaa attaacgatc aactcactgg ctacggtcaa tgggaataca atattcaggc
     9601 aaacaacact gaatcttcaa aaaaccagtc atggacccgt ctggcatttg ccgggctgaa
     9661 atttgcagat tacggttctt tcgattacgg acgtaattat ggcgtaatgt acgacatcga
     9721 aggctggacc gatatgctgc ctgaatttgg cggtgactct tataccaatg cagacaactt
     9781 tatgactggt cgtgccaacg gcgtcgcgac ttatcgtaat actgatttct tcggtctggt
     9841 aaatggtctg aacttcgcgg tgcagtatca aggtaacaac gaaggtgcca gtaatggtca
     9901 ggaaggcacc aacaacggac gtgatgttcg ccatgaaaat ggtgacggct ggggtctttc
     9961 cacaacatat gatttaggca tgggctttag cgctggtgcg gcatacacct catctgaccg
    10021 caccaatgac caggttaacc atactgcggc gggtggtgat aaagcagacg cgtggactgc
    10081 tgggctaaaa tacgatgcta acaatattta cctggcaacc atgtattcag aaacgcgtaa
    10141 tatgaccccg tttggcgaca gcgattatgc tgttgcaaac aaaacccaga actttgaagt
    10201 cactgcacag taccagtttg attttggtct gcgtccggca gtctctttcc tgatgtctaa
    10261 aggccgtgac ctgcacgctg ccgatggtgc agacaacccg gcaggtgttg atgataaaga
    10321 tcttgttaaa tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccactta
    10381 tgttgactat aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaacggcat
    10441 ctctaccgat gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc
    10501 tgttatggcg ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac
    10561 tccgtcatta cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga
    10621 ggttttatga acagaacgat tcttgtccct atcgatattt ccgattcaga attaactcaa
    10681 cgcgtgatta gccacgttga ggcagaggca aagattgacg atgcagaggt tcatttcctg
    10741 acggtaatac cttcactgcc ttactatgcc tctctgggtt tagcgtattc cgcagaatta
    10801 ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa
    10861 tttaaactgc caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc
    10921 attctggaat tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg
    10981 gatatcacca cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc
    11041 tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat
    11101 ttcgcaaatg tgctgacatt ttcccctcta atccgtacca tacacgccac agttttatat
    11161 cagatttctt atgctgggcg ttccggcatg acgcatactc ttctgatgcc atataacgaa
    11221 ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg
    11281 aacaagtaag ggcaaaaatc acaactatct gacccataag tattttattt atttttcaat
    11341 atgttaaaca ttatcagcga caacaataag ctacgataac ccactctttt tttgcccctt
    11401 ttttgcccct tctacagcgt tttgccccat ttttgccatc aacaattttc ctcataaagt
    11461 catttcaaag cctaatagct gctctacttt tttcctcaat atcatgtcgc cagcaggaaa
    11521 caacctaatc cctgttttag gcctcaaagc tgttaatgat aatcattttc attttgtatg
    11581 ggttatgcta taaaacatag ccgttgctat gtttgttcta attttgctca ctataggtac
    11641 taaattatgc actcaataaa aaaagtaacc atgctcttgg ggtgcctcgc cctcacctgc
    11701 tcgatcgcat ttcaggcaag tgcaactgaa aaattcaagg tcattacaac atttaccatc
    11761 atcgcggata tggccaaaaa cgtggctgga gatgctgcag aagtctcatc cataaccaag
    11821 cctggtgcag aaattcatga gtatcagcct acccctggcg atattaaacg tgcgcagggg
    11881 gcacaactga ttctcgccaa tggtatgaat ctggaattgt ggttccaacg cttttaccag
    11941 catctcaatg gggttccaga agtaattgtc tcttcggggg tgacgccagt agggatcacc
    12001 gaaggaccct atgagggcaa acctaatccc catgcatgga tgtcgccaga taatgctctg
    12061 atttacgtcg ataatattcg tgatgcgttg ataaaatacg atccggcaaa tgcacaaacc
    12121 taccaacgta atgccgatac ttataaagcc aagattaccc aaacccttgc ccccctgcgt
    12181 aagcagatta cggaactccc tgagaatcag cgatggatgg tcaccagtga aggggctttt
    12241 tcttatctcg cacgcgattt ggggctaaaa gagctttatc tgtggccgat taatgccgat
    12301 caacaaggaa caccgcagca ggtacgtaag gttgttgata tagttaagaa aaatcatatc
    12361 ccggcagtct ttagcgagag tacgatttcc gataaaccag cgcgtcaggt tgcgcgtgaa
    12421 accggcgcac actacggcgg tgtgctctat gtcgattccc tgagcacaga aaacggcccg
    12481 gtacctacgt atatcgacct tcttaaggtt actaccagta cgctggtaca gggaattaaa
    12541 gccgggaaaa gggagaaata atgatgcaat ctgcaggcat tgtcgttaat gatgttactg
    12601 tcacctggcg taacgggcac acagcactgc gcgatgcatc cttcacagta ccaagtggat
    12661 ctattgccgc tctggttggg gtaaacggtt ccggaaaatc gacgttgttt aaagcgatta
    12721 tgggatttgt gcgtctgacc agcggaaaga tatctgttct gggtattccc acgcgacagg
    12781 cgctacagaa aaacctggtt gcctacgtcc ctcagtcaga agaggttgac tggtcatttc
    12841 ctgtactggt ggaagatgtg gtgatgatgg gccgctatgg gcatatgggt atgttgcgta
    12901 tagccaaaaa gagggatcgt cagattgtca ccgatgcgct ggaacgtgtc gatatggtgg
    12961 attttcgcca tcgtcaaatc ggcgagcttt ccggtggaca aaagaaacgc gtctttctgg
    13021 cgagagcgat tgcacagcag ggtgatgtga tcctgctgga tgaacctttt accggcgttg
    13081 atgtgaaaac cgaagcgaaa atcatcagcc tgttgaggga gttacgcgcg gaaggcaaaa
    13141 cgatgcttgt ctcaacgcac aatctcggct cagtcacgac attttgcgat tatacggtca
    13201 tggtcaaagg caccgtcctg gcaagcgggc cgacagacac cacttttaca gccgagaacc
    13261 tggagctggc ttttagcggc gtactgcgcc atgtcacact taacgactcg gaagaaagca
    13321 ttattactga cgatgaacgc ccttttgtgg cacatcgacc gtcagcggtg cagagggaag
    13381 caagatgaac gtgctactgg aacccttcag ctatgaatat atgctcaatg cgatgtgggt
    13441 ctcggcaatg gtcggcggtc tctgcgcttt cctgtcatgc tatctgatgc taaaaggctg
    13501 gtcgctgatt ggcgatgcgc tgtcgcactc tattgtcccg ggtgttgcgg gggcatatat
    13561 gctggggctt ccgttttctc tgggagcctt cttttccggc ggactggcag cgggcagcat
    13621 gctgtttctt aaccaacgca cccgcttaaa agaagatgcc atcatcggcc tgatcttctc
    13681 gtcctttttt ggcctggggc tgtttatggt gtcattgaac ccgacatccg tgaacattca
    13741 gaccatcgtc ctgggcaata ttctggcaat cgatccggcg gatatcctgc aactcactat
    13801 cattggcatt ctctcgatca tcgtgctttt tttcaaatgg aaagatctta tggtgacctt
    13861 tttcgacgag aatcacgccc gtgctattgg attacatccg ggcagattaa aaatcctctt
    13921 cttcaccctg ctgtcggttt cgaccgttgc ggcgctgcaa acggttggtg cctttctggt
    13981 gatctgtctg gtggtgactc ccggtgcaac agcatggttg ttaaccgatc gttttccccg
    14041 cctgctcata attgctgtaa ctattggcag tgtaaccagt ttcctcggcg cgtgggtcag
    14101 ttactttctg gacggcgcga caggtggaat catcgtggtg gcgcagacac tgctgtttct
    14161 tttagcattt gtatttgcgc ccacccatgg acttttggca aatcgtcggc gtgcgcataa
    14221 agcgctggag gatcgctcat gatggcatta ttactggagc cactgcagtt cacctttatg
    14281 agtcacgcac tgctgatttc actggtagtt tccataccct gtgcgctgct gtcggtcttt
    14341 ctggtcctga aaggttgggc actgatgggt gacgcgatga gtcatgcggt ttttcctggc
    14401 atcgtgctta cctggattct ggggttacct ctggctacag gggcattcgt cgccggagta
    14461 ttctgcgcgg tggcgacggg atacctgaaa gacaacagcc gaatcaagca agatacagtt
    14521 atggggattg ttttttctgg catgtttgcc gcaggtctga tcttgtatat tgcaatcaaa
    14581 ccagacgtac atcttgacca tattcttttc ggcgacatgc tcgggataac catcggcgat
    14641 ataatccaga cggtgattat tgccgggctg gttacacttg ttattagcgc aaaatggcgg
    14701 gattttttgc tgttcagctt tgattatcaa caggcgcagg taagcggttt gcatacgaga
    14761 tggctgcatt atgggctgct gtgcatggtt tccctgacca ttgtggcgac gctgaaagca
    14821 gtaggtatca ttctctctat ttctctgctc attgcgcctg gcgccattgc agtactcctg
    14881 acacaacgct ttcatattgc gctcctactg gcgactggca tatcggtaat agtgtcaatg
    14941 actggtgtct ggctgtcctt ttttatcgac agcgcaccag ctccgacgat tgtcgtctta
    15001 ttcgcggtcg tgttcatcat gacgtttacc gttaccagca tcaacgcacg cacaaaggga
    15061 aacgcccata cacaggatct gttatcaccc aattagcaca acatccgagg acagcatcgt
    15121 ccgccagcct acagaagtcc ggaaagccat ggaaggtcgt tgggaggagg tacgaattct
    15181 atcatgcaaa aaatacgtaa aatc
//
LOCUS       DAJTXQ010000101        14939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000101 DAJTXQ010000000
VERSION     DAJTXQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.101"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OO515_002987"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OO515_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6576859.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OO515_002988"
     CDS             complement(1608..1931)
                     /locus_tag="OO515_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6576860.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO515_002989"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO515_002989"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6576861.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO515_002990"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO515_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6576862.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO515_002991"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO515_002991"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6576863.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO515_002992"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO515_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6576864.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OO515_002993"
     CDS             7531..8568
                     /locus_tag="OO515_002993"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6576865.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO515_002994"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO515_002994"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6576866.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OO515_002995"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OO515_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6576867.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO515_002996"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO515_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6576868.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OO515_002997"
     CDS             10739..11620
                     /locus_tag="OO515_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6576869.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO515_002998"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO515_002998"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6576870.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OO515_002999"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OO515_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6576871.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14939)
                     /locus_tag="OO515_003000"
     CDS             complement(14017..>14939)
                     /locus_tag="OO515_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6576872.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3605 a         3780 c         3912 g         3642 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaaccc
//
LOCUS       DAJTXQ010000102        14395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000102 DAJTXQ010000000
VERSION     DAJTXQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.102"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="OO515_003001"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="OO515_003001"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6576873.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO515_003002"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO515_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6576874.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO515_003003"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO515_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6576875.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO515_003004"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO515_003004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6576876.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO515_003005"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO515_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6576877.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OO515_003006"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="OO515_003006"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6576878.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OO515_003007"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="OO515_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6576879.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OO515_003008"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="OO515_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6576880.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="OO515_003009"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="OO515_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6576881.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="OO515_003010"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="OO515_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6576882.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSSVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="OO515_003011"
     CDS             complement(11402..11899)
                     /locus_tag="OO515_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6576883.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="OO515_003012"
     CDS             complement(11896..12258)
                     /locus_tag="OO515_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6576884.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="OO515_003013"
     CDS             complement(12248..12595)
                     /locus_tag="OO515_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6576885.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="OO515_003014"
     CDS             12704..13153
                     /locus_tag="OO515_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576886.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>14395)
                     /locus_tag="OO515_003015"
     CDS             complement(13402..>14395)
                     /locus_tag="OO515_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6576887.1"
                     /translation="DAPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQ
                     GVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVH
                     PSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQK
                     RKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVR
                     AKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQ
                     SKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3397 a         3975 c         3729 g         3294 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctcca gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggcc ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggca ggctgaccgt acccgctgct gactttgaca tgttgctgac
    13801 cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttgtg
    13861 aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg
    13921 cttctgccag ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag
    13981 cttcttctgg tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt
    14041 ggcgagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
    14101 tgatacttca ctttctgtct gaatactgat gtaccactta ccgcaggact ggctgacagt
    14161 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agcccagttt
    14221 cggcagaaaa atacggctgt tttcctgatc gagcttaacg ccctgcgggt agcggaatac
    14281 atcattctgt ccccgctttt tgaatcgggg aaaagcagcc cgattctgga agaagttttt
    14341 gtaagcccgc tcaaggtctt tcagtgactg ttgcaatggc tgtgacgggg catct
//
LOCUS       DAJTXQ010000103        14369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000103 DAJTXQ010000000
VERSION     DAJTXQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.103"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..696)
                     /locus_tag="OO515_003016"
     CDS             complement(<1..696)
                     /locus_tag="OO515_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6576888.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="OO515_003017"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="OO515_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6576889.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="OO515_003018"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="OO515_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6576890.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="OO515_003019"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="OO515_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6576891.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="OO515_003020"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="OO515_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6576892.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="OO515_003021"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="OO515_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6576893.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="OO515_003022"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="OO515_003022"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6576894.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="OO515_003023"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="OO515_003023"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6576895.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="OO515_003024"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="OO515_003024"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6576896.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="OO515_003025"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="OO515_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6576897.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="OO515_003026"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="OO515_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6576898.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="OO515_003027"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="OO515_003027"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6576899.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="OO515_003028"
     CDS             10119..10499
                     /locus_tag="OO515_003028"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6576900.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="OO515_003029"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="OO515_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6576901.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OO515_003030"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OO515_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6576902.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="OO515_003031"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="OO515_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="OO515_003032"
     CDS             complement(12845..14032)
                     /locus_tag="OO515_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6576903.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="OO515_003033"
     CDS             complement(14032..>14369)
                     /locus_tag="OO515_003033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576904.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAJTXQ010000104        14153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000104 DAJTXQ010000000
VERSION     DAJTXQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.104"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..222)
                     /locus_tag="OO515_003034"
     CDS             complement(<1..222)
                     /locus_tag="OO515_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6576905.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSA"
     gene            379..555
                     /locus_tag="OO515_003035"
     CDS             379..555
                     /locus_tag="OO515_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6576906.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1181..1480
                     /locus_tag="OO515_003036"
     CDS             1181..1480
                     /locus_tag="OO515_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576907.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1527..2414)
                     /gene="rarD"
                     /locus_tag="OO515_003037"
     CDS             complement(1527..2414)
                     /gene="rarD"
                     /locus_tag="OO515_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6576908.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2466..2933)
                     /locus_tag="OO515_003038"
     CDS             complement(2466..2933)
                     /locus_tag="OO515_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6576909.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3098..3967
                     /gene="pldA"
                     /locus_tag="OO515_003039"
     CDS             3098..3967
                     /gene="pldA"
                     /locus_tag="OO515_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6576910.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4100..5929
                     /gene="recQ"
                     /locus_tag="OO515_003040"
     CDS             4100..5929
                     /gene="recQ"
                     /locus_tag="OO515_003040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6576911.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5993..6610
                     /gene="rhtC"
                     /locus_tag="OO515_003041"
     CDS             5993..6610
                     /gene="rhtC"
                     /locus_tag="OO515_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6576912.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6672..7292)
                     /gene="rhtB"
                     /locus_tag="OO515_003042"
     CDS             complement(6672..7292)
                     /gene="rhtB"
                     /locus_tag="OO515_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6576913.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7403..8425
                     /gene="pldB"
                     /locus_tag="OO515_003043"
     CDS             7403..8425
                     /gene="pldB"
                     /locus_tag="OO515_003043"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6576914.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8433..9233
                     /gene="yigL"
                     /locus_tag="OO515_003044"
     CDS             8433..9233
                     /gene="yigL"
                     /locus_tag="OO515_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6576915.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9309..10208
                     /gene="bioP"
                     /locus_tag="OO515_003045"
     CDS             9309..10208
                     /gene="bioP"
                     /locus_tag="OO515_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6576916.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10096..11049)
                     /gene="metR"
                     /locus_tag="OO515_003046"
     CDS             complement(10096..11049)
                     /gene="metR"
                     /locus_tag="OO515_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6576917.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11285..13546
                     /gene="metE"
                     /locus_tag="OO515_003047"
     CDS             11285..13546
                     /gene="metE"
                     /locus_tag="OO515_003047"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6576918.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13831..14076)
                     /locus_tag="OO515_003048"
     CDS             complement(13831..14076)
                     /locus_tag="OO515_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6576919.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3445 a         3423 c         3688 g         3597 t
ORIGIN      
        1 ggcactaaaa gcaacattaa tagaacctgg gccagggaaa tggtatttaa taagtctgta
       61 aggatcaact tttgctgaga tatcgcttag gccgagtgag agaaagtctt gttctaccat
      121 atcaccatat tgcatatcat cagcagaaaa atcatcgaat aaactaatag tttcgaaaag
      181 tgtacaggga atattaattg gcggaagtaa tatgttattc atttgcattt gccttggtta
      241 tatttaagct caagatcaat tcgagaacaa atgctagggt aaaaaaatat ttaaaaaaga
      301 agtacacttc acattttttc attacatctc tgaagagaac ctcgttaatg attggtcttg
      361 ctcattgtta ataacaaaat gttgaattta ccgattagga aactttttcc tacgcggatt
      421 gcctccagct atggcatgaa ctttgagttt atgccagaac tgaagtggag ctttggctac
      481 cctggcgcga ttatctttat gatccttgcg ggcctgacac cgtatctgta ctttaagcgt
      541 aagaactggc tgtaactata cgatgtcgct gtgagtaagt tagtgtgctg acagcgacat
      601 tttagccgag ggaatcgcag ataacataat tgttgtgaat aatatccatg ccacgttgag
      661 ttggacgctt aatgactcac atctcacccc ttcaattgct gcagagataa tagaaaaaag
      721 tgtcaaaccc aatgaaatca atacaaagta gattgcctta catccagtat ctcttattct
      781 ttttgctcca atattgatat ttacataggt attattaata accagaataa aactcaaagc
      841 caggttgata tcattcaaag ttattaatat tgaccatgat gttaatgtat agattatgaa
      901 ctgaaagcgt gataaacgct caagcctgga tgggtagaat atatttctga tggatgattt
      961 gaacattttt acattgtttt atatatataa ttgttttgaa tatatattac ctgaattatg
     1021 tggtttgcat atatatatgc aatatagcag tctttgcatt gataactata cagtagtaat
     1081 ttagagtgtg taagttttta ttgatggggt atattttaat taaatataat tgaaatgtat
     1141 ttttattcta taaacaatat cttgggtgaa tggattctat atgggaaatg gtaacttttg
     1201 gcagttgttc ttgggtaaag aaccttacag caaaaaatct gattttgtta aggcatggtt
     1261 attgtggtgg gctattacca tccttgttct atttatttta agcctgacta cgatattaaa
     1321 tttaagaagt ataggtatag ggttaagttt tgggttttgt caaatccttg gtagatatac
     1381 gatgtctcta ggatggagta agtactgggg agttttgggg gtatttcctg taacaaatat
     1441 tgttcttttt cttatcttaa tttttttcaa aaaaagataa ataagtcagt gggtaggctc
     1501 tttaaacggc ctacccatgt taattcttaa ctttttctac gctgcgtata aatcgcatcc
     1561 atcacaaaaa ttgccagcgc cacccaaata aaggcgaaag tcaccatctt atcggcaccc
     1621 ggtttttcac cataaaacgt cacagccagc aggaacatca gcgtcgggcc aatgtactgg
     1681 aaaaagccta acgttgagag acgcaagcgc gtggcagcgg cggtaaaaca caacagcggt
     1741 acggtagtga caataccggc ggcgatcagc agtaaattca gcgacatcgg gttttgcccc
     1801 atatggctgg ttgagctgtc ggcaatagca aacaggtaaa ttgccgccac gggcagcagc
     1861 cacatggttt cgattaacat gccggtttgc gcttcaacgg caatcttctt gcgtaccaga
     1921 ccgtagaagg caaaactaaa tgccagtccc agcgcgataa taggtagcga accaaaagtc
     1981 cacagctgga ctaacacgcc acatatcgcc agaatcaccg ccagccattg catccggcgg
     2041 aatcgctcgc cgaggaaaat catccccagc acaatgttca ccagcgggtt aataaagtaa
     2101 ccaaggctcg cttccagcat atggtgattg ttcaccgccc agataaacag tagccagttg
     2161 ccaccaatca gcacggcaga gactgccagc ataaaaattt tctgtggcgt ctgaatcagc
     2221 gtttttaaat aggaccactg gcggcaaatg ctcatcagca ccaccataaa gaaaaacgac
     2281 cagatcacgc gatgcgtcag gatttcatcg gcgggcacgt agtaaatcaa cttgaagtac
     2341 gctggcgcta taccccaaat aaaataagcg gcaagagcga gtaatacgcc ctgccgcgtt
     2401 tgttttgcat ccatcgggaa tactcatttt taattggtaa cagcagttta cctgctttta
     2461 tgtcttcaac ctaccatata ggtggcggtg gcactggcaa tataaagctg ttcttcattg
     2521 tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac gcaacaggct actggtagca
     2581 gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc gaagatcaat ggtccccatc
     2641 cgcgatagcc gctggcgtag ttcatcttca ctgatggttt cgtggcgggt taaggtactt
     2701 cccacgcaca ccagaccggc ggcgacatcc agcgccgacg caatgacccc gccgtgcaaa
     2761 atgctttgcg cccagttgcc caccatcatt ggctgatttt taaaggccag ctgtgcgaac
     2821 tctttttcgt aacgctccag ttccatcccc aatgcgcggt taaatggcat gtgataaaca
     2881 aacatctcac ccactaattt cagggcttgt tcagcggtca gtacggcaga catatcatcc
     2941 ttacacttca ttggttaatg aaatgttgat tttatgcttc tttgttgttg gtttctactt
     3001 taggaaggga taactaacgg ctatggagtt aagtatgtaa aatagcccgc agaaaaatat
     3061 tcaccttatc aataattcgt tacggagaac acgaccgatg cggactctgc agggctggtt
     3121 gttgccggtg tttatgttgc ctatggcagt atatgcacaa gaggcaacgg tgaaagaggt
     3181 gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat atgctgcagg agcatgacaa
     3241 tccgttcacg ctctatcctt atgacaccaa ctacctcatt tacacccaaa ccagcgatct
     3301 gaataaagaa gcgattgcca gttacgactg ggcggaaaat gcgcgtaagg atgaagtaaa
     3361 gtttcagttg agcctggcat ttccgctgtg gcgtgggatt ttaggcccga actcggtgtt
     3421 gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc aatagcgaag agtcttcacc
     3481 gtttcgtgaa accaactacg aaccgcaatt gttcctcggt tttgccaccg attactgttt
     3541 tgcaggttgg acgctgcgcg atgtggagat ggggtataac cacgactcta acgggcgttc
     3601 cgacccgacc tcccgcagct ggaaccgcct ttatactcgc ctgatggcag aaaacggtaa
     3661 ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat actgacgata acccggatat
     3721 caccaaatat atgggttact accagcttaa aatcggctat cacctcggtg atgcggtgtt
     3781 gagtgcgaaa ggacagtaca actggaatac cggttacggc ggcgcggagt taggcttaag
     3841 ttacccgatc accaaacatg tgcgccttta tactcaggtt tacagcggct atggcgaatc
     3901 gctcatcgac tataacttca accagacccg tgtcggtgta ggagttatgc taaacgactt
     3961 gttttgatga acggttgagt ggttggcaaa tctggaaccc agcatccagg attaccctct
     4021 cagagactaa aagcattgca gtttctcgcg caggcgctga aaatagcgcc tgtttttatt
     4081 tcaggcaatc ggggggaatg tggcgcaggc ggaagtgttg aatctggagt ccggagctaa
     4141 acaggtttta caagaaacct ttggctacca acagtttcgc cccggccagg aagaaattat
     4201 cgacactgtg ctttccggtc gcgattgcct ggtcgtcatg cccaccggtg gcggaaaatc
     4261 cctttgctat caaattcctg ccttattgct aaacggcctt accgtggttg tttcaccgct
     4321 gatttcgttg atgaaagatc aggtggatca actgcaagcc aacggcgtgg cggcggcgtg
     4381 ccttaactcg acgcaaaccc gcgagcagca acttgaagtg atgacaggct gccgcaccgg
     4441 gcaaattcgt ctgctttata tcgccccgga acgcctgatg ctggataact ttcttgagca
     4501 tctggcgcac tggaatccgg tgttattagc cgttgatgaa gcgcactgta tctcccaatg
     4561 gggccacgat ttccgcccgg aatatgccgc gcttggtcag ttacgccagc gtttcccgac
     4621 cctgccgttt atggcgctga ctgccacagc cgacgacacc acgcgccagg atatcgtgcg
     4681 cttgctgggg ctgaacgatc cgctgattca aatcagcagt tttgaccgtc cgaatattcg
     4741 ctacatgctg atggagaaat tcaaaccgct cgatcagttg atgcgctacg tgcaggaaca
     4801 gcgcggtaag tccggcatta tctactgcaa cagccgggcg aaagtagaag acaccgctgc
     4861 gcgcctgcaa agcaagggta ttagcgcggc ggcctatcat gccgggctgg aaaataatgt
     4921 ccgcgccgac gtgcaggaga aattccagcg cgatgacctg caaattgtgg tggcgacggt
     4981 ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc gtggtccact ttgatattcc
     5041 gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc gggcgtgatg gcctgcctgc
     5101 ggaagcgatg ctgttttacg atccggctga tatggcgtgg ctgcgccgtt gtctggaaga
     5161 gaagccgcag gggcagttgc aggatattga gcgccacaaa ctcaatgcga tgggcgcgtt
     5221 tgccgaagcg caaacttgcc gtcgtctggt attgctgaac tattttggcg aagggcgtca
     5281 ggagccgtgc gggaactgcg atatctgcct cgatccgccg aaacagtacg acggttcaac
     5341 cgatgctcag attgcccttt ccaccattgg tcgtgtgaat cagcggtttg ggatgggtta
     5401 tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc cgcgactatg gtcatgacaa
     5461 actgaaagtc tatggcatgg gccgtgataa aagccatgaa cattgggtga gcgtgatccg
     5521 ccagctgatt cacctcggcc tggtgacgca aaatattgcc cagcattctg ccctacaact
     5581 gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct ttgcaacttg ccgtgccgcg
     5641 tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc ggcggcaact atgatcgtaa
     5701 actgttcgcc aaattacgca aactgcgtaa atcgattgcc gatgaaagta atgtcccgcc
     5761 gtacgtggtg tttaacgacg caaccttgat tgagatggct gaacagatgc cgatcaccgc
     5821 cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag ctggaacgct ttggtaaacc
     5881 gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac gaagagtagt cagcagcata
     5941 aaaaagtgcc aggatgaaga ctccgtaaac gtttcccccg cgagtcaaat gtatgttgat
     6001 gttatttctc accgtcgcca tggtgcacat tgtggcgctt atgagccccg gtcccgattt
     6061 cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa gaagcgatga tgggcgtgct
     6121 gggcattacc tgcggtgtaa tggtttgggc tgggattgcg ttgcttggcc tgcatttgat
     6181 tatcgaaaaa atggcctggc tgcatacgct tatggttggc ggtggcctgt atctctgctg
     6241 gatgggttac cagatgctgc gcggcgcgct gaaaaaagag gcggtttctg cacctgcgcc
     6301 acaggtcgag ctggcgaaaa gtggacgcag tttcctgaaa ggtttactga ccaatctcgc
     6361 taatccgaaa gcgattatct actttggctc ggtgttctca ttgtttgtcg gtgataacgt
     6421 tggcactacc gcgcgctggg gcatttttgc gctgatcatt gtcgaaacgc tggcgtggtt
     6481 tatcgtcgtt gccagcctgt ttgccctgcc gcaaatgcgc cgtggttatc aacgtctggc
     6541 gaagtggatt gatggttttg ccggggcgtt atttgccgga tttggcattc atttgattat
     6601 ttcgcggtga tgccagacgc gtcttcagag taagtcggat aaggcgttta cgccgcatcc
     6661 gacattattt ttcacgcatg cctcgccgat gctaacagcg ctcccaccag cataaacaac
     6721 gagccgaaaa tcttattcag cgccttcatc tgctttggtc ctttaatcca tagagcaatc
     6781 cgttgagcaa gggtggcgta accgatcatc acaataatat cgaccacaat agtggtgacg
     6841 ccgagcacga tatactgcat cagttgcggc tgttgcggca tgatgaattg cggaaatagc
     6901 gccgccagaa acacaatact tttgggattg gtgagattca caaaaactgc gcgctggaac
     6961 aaatgtcgac gcgattgagt agaggccagc gatttaaggt caattgcacc agcggcgcgc
     7021 cactgctgga ttcccagcca aatcaagtaa gccgcgcctg cccacttcaa cacttcaaac
     7081 gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc caaccagcac aatatgaatc
     7141 gccagtccgg tctgaagccc agcaatagac gccaccgcgc cgcgataacc gtggttgagc
     7201 gaggtggtca tagtgttgat tgcaccagag cctggcgaca ggcttaaaat gatcgatgtc
     7261 agcaggtagg caaaccacca ttctaaggtc atgatgaact cccggtgtgt ctatttttgt
     7321 gccacaatac gctactgtcg cagcgttgtg tcaggcacgc taaaaaaaac gattttacgt
     7381 ggtttaagag gcagattacc cgatgtttca gcagcaaaaa gactgggaaa caagagaaaa
     7441 cgcgtttgct gcttttacca tgggaccgct gactgatttc tggcgtcagc gtgatgaagc
     7501 agagtttact ggtgtggatg acattccggt gcgctttgtc cgttttcgcg cacagcacca
     7561 tgaccgggtg gtagtcatct gcccggggcg tattgagagc tacgtaaaat atgcggaact
     7621 ggcctatgac ctgttccatt tggggtttga tgtcttaatc atcgaccatc gcgggcaggg
     7681 acgttccggt cgcctgttag ccgatccgca tctcgggcat gttaatcgct ttaatgatta
     7741 tgttgatgat ctggcggcat tctggcagca ggaggttcag cccggtccgt ggcgtaaacg
     7801 ctatatactg gcacattcga tgggcggtgc gatctccaca ttatttctgc aacgccatcc
     7861 aggtgtatgt gacgccattg cgctaactgc gccaatgttt gggatcgtga ttcgtatgcc
     7921 gtcatttatg gcacggcaga tcctcaactg ggccgaagcg catccacgtt tccgtgatgg
     7981 ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt gctatcaacg tactgaccca
     8041 cagcagacag cgatatcgac gtaacttacg cttctatgct gatgacccaa cgattcgcgt
     8101 cggtgggccg acctaccatt gggtacgcga aagtattctg gctggcgaac aggtgttagc
     8161 cggtgcgggt gatgacgcca cgccaacgct tctcttgcag gctgaagagg aacgcgtggt
     8221 ggataaccgc atgcatgacc gtttttgtga actccgcacc gccgcgggcc atcctgtcga
     8281 aggaggacga ccgttggtaa ttaaaggtgc ttaccatgag atcctttttg aaaaggacgc
     8341 aatgcgctca gtcgcgctcc acgccattgt tgattttttc aacaggcata actcacccag
     8401 cggaaaccgc tctacagagg tttaaatttc ttatgtacca ggttgttgcg tctgatttag
     8461 atggcacgtt actttctccc gaccatacgt tatcccctta cgccaaagaa accctgaagc
     8521 tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg tcgtcaccac gttgatgtgg
     8581 ggcaaattcg cgataatctg gagatcaagt cgtacatgat cacctccaac ggcgcgcgcg
     8641 ttcacgatct ggatggcaat ctgatttttg ctcataacct ggatcgcgac attgccagcg
     8701 atctgtttgg cgtagtcaac gacaatctgg acatcattac taacgtttat cgcgacgacg
     8761 aatggtttat gaatcgccat cgcccggaag agatgcgctt ttttaaagaa gcggtgttca
     8821 aatatgcgct gtatgagcct ggattactgg agccggaagg cgtcagcaaa gtgttcttca
     8881 cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc gattaacgct cgttggggcg
     8941 atcgcgtcaa cgttagtttc tctaccttaa cctgtctgga agtgatggcg ggcggcgttt
     9001 caaaaggcca tgcgctggaa gcggtggcga agaaactggg ctacagcctg aaggattgta
     9061 ttgcgtttgg tgacgggatg aacgatgccg aaatgctgtc gatggcgggg aaaggctgca
     9121 ttatgggcag tgcgcaccag cgtctgaaag accttcatcc cgagctggaa gtgattggta
     9181 ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact ctatttatcg taatcgttct
     9241 ttatttggtc agttgtcaac ctgatacttc gctacaatgg atactcgtta atcaaagagt
     9301 tttccattgt ggcgctactt atcatcacca cgattctgtg ggccttctcc tttagctttt
     9361 atggcgagta ccttgcgggg cacgtcgata gctattttgc ggtgctggtg cgcgttggcc
     9421 tggcggcact cgtttttctg ccgtttctgc gtacccgtgg caatagcctg aaaacggtcg
     9481 gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat gtatatgctg agtttccgcg
     9541 cttatctcta cctgacggtc tccgaattgt tgctgttcac cgtactgacg ccgctctaca
     9601 tcacgctgat ttatgacatc atgagtaagc gccgtctgcg ctggggctat gcttttagcg
     9661 ccttgctggc ggtgattggt gccgggatta ttcgctatga tcaggttacc gaccatttct
     9721 ggactggctt gttgctggtg caactctcca atatcacttt tgccatcggc atggtgggtt
     9781 acaaacgcct gatggaaact cgcccgatgc cgcagcataa cgcctttgcg tggttctatc
     9841 ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct gggaaatgcg cagaaaatgc
     9901 cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg cgtggtggct tccgggattg
     9961 gctactttat gtggaactac ggcgcgacgc aggtggacgc cggaacgctg ggcattatga
    10021 ataatatgca cgttccggca gggctgctgg taaacctggc tatctggcac caacagccgc
    10081 actggccaac gttcattaca ggcgcgctgg tgatcctggc ctcactgtgg gtgcatcgta
    10141 agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg caggcgtgat tgcgcgctga
    10201 gcgaataaat gcttctgtaa tcggctgacg ctgctcgcca tcgcgcacgg cggcgtacag
    10261 tcggctccac aagccttcgc ccagggtttt ggtcaccacc agaccttggc gctcaaaact
    10321 ctctactacc caatgcggta gcgcggcaat acccatccgc gcggcaacca tctgaatcaa
    10381 caataaggtg ttatcgacgc ttttcagtga cgggctgacg cctgccggct gaagaaaatg
    10441 ccgccagaca tccagtcgac tacgctgcac cggataaatc aacagcgtct cgctggcgag
    10501 atcttccggt gtaattcgcg ttttcgccgc cagtggatgg tcaggtgcta acaccagacg
    10561 cacttcatag tcgaacatcg gcgaataatg caggccactg cgcggcagga tatcggaggt
    10621 catcaccaga tccagctcgc cctgttgcaa cgcaggctgc gggtcaaacg tcacgcccga
    10681 tttaaaatcc atctctacct gcggccagtt cttatggaaa ttttctaacg cgggcgtcag
    10741 ccactgaata cagctatggc actcaatggc aatgcgcaga cgcgtctgct gcggttcatt
    10801 gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg tttgccagtt gcaacaggat
    10861 ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc acaaatagcc ggaagccaag
    10921 gcgttgttcc agatcgctaa actggtgaga cagggcggat tgcgtctgat gcaacgtcgc
    10981 cgcagcggct gcgagagagc cgcagttccg caacgcttgt agcgttttca ggtgttttac
    11041 ttcgatcatg aaagtccttc acttcggcat gaataatttg cgcttgagga atatacagta
    11101 accgccaatt atggatgtgt aaacatctgg acggctaaaa tccttcgtct tttaaattta
    11161 tggtgcgttg gctgcgtttc tccaccccgg tcacttactt cagtaagctc ccggggatga
    11221 ataaacttgc cgccttccct aaattcaaaa tccataggat ttacatataa ttagaggaag
    11281 aaaaatgaca atattgaatc acaccctcgg tttccctcgc gttggcctgc gtcgcgagct
    11341 gaaaaaagcg caagaaagtt attgggcggg gaactccacg cgtgaagaac tgctgacagt
    11401 agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag caagcgggta tcgacctgct
    11461 gccggtgggc gattttgcct ggtacgatca tgtactgacc accagtttgc tgctgggtaa
    11521 tgttccgcca cgtcatcaga acaaagacgg ttcggtagat atcgacaccc tgttccgtat
    11581 tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca gcggaaatga ccaaatggtt
    11641 taacaccaac tatcactaca tggtgccgga gttcgttaaa ggccaacagt tcaaactgac
    11701 ctggacgcag ctgctggagg aagtggacga ggcgctggcg ctgggccaca acgttaagcc
    11761 tgtgctgctg gggccggtta cttacctgtg gctggggaaa gtgaaaggtg aacagtttga
    11821 ccgccttagc ctgctgaacg acattctgcc ggtttatcag caagtgctgg cagaactggc
    11881 gaaacgcggc atcgagtggg tacagattga tgaacccgcg ctggtactgg aactaccaca
    11941 ggcgtggctg gacgcataca aacccgctta cgacgcgctc cagggacagg tgaaactgct
    12001 gctgaccacc tattttgaag gcgtaacgcc aaatctcgac acgattactg cgctgcctgt
    12061 tcagggtctg catgttgatc tcgtacatgg taaagatgac gttgctgaac tgcacaagcg
    12121 cctgccttct gactggttgc tgtctgcggg tctgatcaat ggtcgtaacg tctggcgcgc
    12181 cgatcttacc gagaaatatg cgcaaattaa ggacattgtc ggcaaacgtg atttgtgggt
    12241 ggcatcttcc tgctcgttgc tgcacagccc catcgacctg agcgtggaaa cgcgtcttga
    12301 tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa tgccatgaac tagcactgct
    12361 gcgcgatgcg ctgaacagtg gtgacacggc agctctggca gagtggagcg ccccaattca
    12421 ggcgcgtcgt cactctactc gtgtacataa tccggcggta gaaaagcgtc tggcggcgat
    12481 caccgcccag gacagccagc gtgcgaatgt ctatgaagtg cgtgctgaag cccagcgtgc
    12541 acgttttaaa ctgccagcgt ggccgaccac cacgattggt tccttcccgc aaaccacgga
    12601 aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc gacgccaata actaccgcac
    12661 gggcattgcg gaacatatca ggcaggccat tgttgaacag gaacgtttgg gactggatgt
    12721 gctggtacat ggcgaggccg agcgtaatga catggtggaa tactttggcg agcacctcga
    12781 cgggtttgtc tttacgcaaa acggttgggt acagagctac ggttcccgct gcgtgaagcc
    12841 accgattgtt attggtgacg ttagtcgccc ggcaccgatt accgtggagt gggcgaagta
    12901 tgcgcaatct ctgactgata aaccggtgaa agggatgctg actggcccgg tgactattct
    12961 ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc atcgccaaac agattgcgct
    13021 ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga attggcatca tccagattga
    13081 cgaaccggct ctgcgcgaag gtttaccgct gcgtcgtagc gactgggatg cgtatctcca
    13141 gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg aaagatgaca cacaaatcca
    13201 cactcacatg tgttattgcg agttcaacga catcatggat tcgattgcgg cactggacgc
    13261 agacgtcatc accatcgaaa cctcgcgttc tgacatggag ttgctggagt cgttcgaaga
    13321 gtttgattat ccgaatgaaa tcggtccagg cgtctatgac attcactcgc caaacgtacc
    13381 gagcgtggaa tggattgaag ccttgctgaa gaaagcggca aaacgcattc cggcagagcg
    13441 cctgtgggtt aacccggact gtggcctgaa aacgcgcggc tggccagaaa cccgcgcggc
    13501 actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg ggatgatgaa tattaacgag
    13561 tcactgtaaa ttgcagtacg atgaaagagg caaaactgcc tgatactagg cctacataat
    13621 ttatgcaatt tattgaattt ttgggttttg ttggccggac aaggcgttca cgccgcatcc
    13681 ggcatgaaca acgcgcactt tgccaataat ctgatggctt ccatcaccgg aagccatttt
    13741 gctatctgcg cccgttcaga tacagaaata accgaatcat ttttacgcaa aatataatcc
    13801 tgagcgattt gttcagtaat caacgctacg ttatttattc tcacttttca cgaaattgta
    13861 tcccttttca gtaacgtggt agtgaggcat agacttatgt ccgctatcct caataataac
    13921 caaaccctcg ttagctaatt tgttaacaat gttaacaaca ttaccgaatc ctggaatttg
    13981 ccttatggat agtgtgcgat cgagattcat ccagttccag tttatatctt tctcagccat
    14041 aattttcagt acgttaattt cagtttcagt tatcatagtc aagtgcatcc tggtggtata
    14101 agtcattaag ttctccctta agtttataat cttctagagt agcagtatgc gta
//
LOCUS       DAJTXQ010000105        14107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000105 DAJTXQ010000000
VERSION     DAJTXQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.105"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="OO515_003049"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="OO515_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6576920.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="OO515_003050"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="OO515_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6576921.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="OO515_003051"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="OO515_003051"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6576922.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="OO515_003052"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="OO515_003052"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6576923.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="OO515_003053"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="OO515_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6576924.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="OO515_003054"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="OO515_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6576925.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="OO515_003055"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="OO515_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="OO515_003056"
     tRNA            complement(6687..6776)
                     /locus_tag="OO515_003056"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="OO515_003057"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="OO515_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6576926.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="OO515_003058"
     tRNA            7768..7843
                     /locus_tag="OO515_003058"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="OO515_003059"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="OO515_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3991 a         3353 c         3170 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta ataacagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAJTXQ010000106        14091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000106 DAJTXQ010000000
VERSION     DAJTXQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.106"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="OO515_003060"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="OO515_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6576927.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="OO515_003061"
     CDS             2273..2662
                     /locus_tag="OO515_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6576928.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="OO515_003062"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="OO515_003062"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6576929.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OO515_003063"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="OO515_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6576930.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="OO515_003064"
     CDS             complement(4321..4521)
                     /locus_tag="OO515_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6576931.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="OO515_003065"
     CDS             4687..5232
                     /locus_tag="OO515_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6576932.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="OO515_003066"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="OO515_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="OO515_003067"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="OO515_003067"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6576933.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="OO515_003068"
     CDS             complement(6529..7353)
                     /locus_tag="OO515_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6576934.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OO515_003069"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="OO515_003069"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6576935.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OO515_003070"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="OO515_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6576936.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OO515_003071"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="OO515_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6576937.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OO515_003072"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="OO515_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6576938.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OO515_003073"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="OO515_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6576939.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OO515_003074"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="OO515_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6576940.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="OO515_003075"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="OO515_003075"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6576941.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAJTXQ010000107        14060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000107 DAJTXQ010000000
VERSION     DAJTXQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.107"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..311)
                     /locus_tag="OO515_003076"
     CDS             complement(<1..311)
                     /locus_tag="OO515_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6576942.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PT"
     gene            complement(449..2095)
                     /gene="groL"
                     /locus_tag="OO515_003077"
     CDS             complement(449..2095)
                     /gene="groL"
                     /locus_tag="OO515_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6576943.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2139..2432)
                     /locus_tag="OO515_003078"
     CDS             complement(2139..2432)
                     /locus_tag="OO515_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6576944.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2708..3964
                     /gene="yjeH"
                     /locus_tag="OO515_003079"
     CDS             2708..3964
                     /gene="yjeH"
                     /locus_tag="OO515_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6576945.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(3986..4456)
                     /gene="fxsA"
                     /locus_tag="OO515_003080"
     CDS             complement(3986..4456)
                     /gene="fxsA"
                     /locus_tag="OO515_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6576946.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            4793..6229
                     /gene="aspA"
                     /locus_tag="OO515_003081"
     CDS             4793..6229
                     /gene="aspA"
                     /locus_tag="OO515_003081"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6576947.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6347..7648
                     /gene="dcuA"
                     /locus_tag="OO515_003082"
     CDS             6347..7648
                     /gene="dcuA"
                     /locus_tag="OO515_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6576948.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7764..8102
                     /gene="cutA"
                     /locus_tag="OO515_003083"
     CDS             7764..8102
                     /gene="cutA"
                     /locus_tag="OO515_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6576949.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8078..9775
                     /gene="dsbD"
                     /locus_tag="OO515_003084"
     CDS             8078..9775
                     /gene="dsbD"
                     /locus_tag="OO515_003084"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6576950.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9812..10387
                     /locus_tag="OO515_003085"
     CDS             9812..10387
                     /locus_tag="OO515_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6576951.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLANQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10494..10569
                     /locus_tag="OO515_003086"
     tRNA            10494..10569
                     /locus_tag="OO515_003086"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10527..10529,aa:Phe,seq:gaa)
     gene            11186..12723
                     /gene="cadC"
                     /locus_tag="OO515_003087"
                     /pseudo
     CDS             11186..12723
                     /gene="cadC"
                     /locus_tag="OO515_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            13088..>14060
                     /gene="cadB"
                     /locus_tag="OO515_003088"
     CDS             13088..>14060
                     /gene="cadB"
                     /locus_tag="OO515_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6576952.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3407 a         3520 c         3384 g         3749 t
ORIGIN      
        1 gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta
       61 ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc
      121 tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt accaatcagc
      181 tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc agcactcaat
      241 tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat
      301 tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc
      361 tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt
      421 ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc
      481 ccataccgcc agcagcgcct aagtcagctg catcattttt cggcaggtcg gtaaccatgc
      541 actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta
      601 ctttggttgg gtccaggata cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt
      661 tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc aacaacagac ggttcttcgc
      721 cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca actttgatac
      781 ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca
      841 gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg
      901 cgtcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca cctactttga
      961 taactgcaac gccgcctgcc agtttcgcta cgcgttcctg cagtttttca cggtcgtagt
     1021 cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt
     1081 cttcacccac gccatcgatg atagtggtgg tgtctttgtt gatcacaaca cgtttagcct
     1141 gacccaggtc ttccagggtt gctttttcca gttccatacc gatctcttca gagatcacgg
     1201 taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg
     1261 gagctttaac tgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca
     1321 gcgcttcgcc ttctacatct tcagcgatga tcagcagcgg tttgcctgct ttggcaacgg
     1381 cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc agcaggatga
     1441 acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt
     1501 agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt
     1561 cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac
     1621 ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct ttagagtcag
     1681 agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaacggct ttgtcgatac
     1741 cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagaccc tcagtgatga
     1801 tagcctgagc cagtacagtt gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttag
     1861 aggcaacttc tttcaccatc tgcgcaccca tgttttcgaa cttgtcttcc agttcgattt
     1921 cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa
     1981 ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt acgtttacgc
     2041 cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct
     2101 ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt acgcttcaac aattgccaga
     2161 atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg
     2221 tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca
     2281 ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct
     2341 gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg
     2401 atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta
     2461 tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg
     2521 atgaggattc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg
     2581 gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt cataagcgaa
     2641 tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggata
     2701 tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg gcctgctatc
     2761 gacgtcatta ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac aggtagcagg
     2821 caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt
     2881 gtttgcgatt ctgggtcgcc actatcccag cgcaggcggt gtcgcgcact tcgtcggtat
     2941 ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg tcattcccgt
     3001 gggtttgcct gccgcgctac aaattgccgc cgggttcggc caggcaatgt ttggctggca
     3061 tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt atatcggtac
     3121 tcgcggtgcc agttccagtg ctaatctaca aaccgttatt gccggactta tcgtcgcgct
     3181 gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct tcccggcacc
     3241 tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt
     3301 cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc
     3361 tcgcgcattg atgattggtc tgctgctggc aggattagtc tactggggct gtacggtagt
     3421 cgtcttacac ttcgacgcct atggtgaaca aatggccgcg gcagcatcgc ttccaaaaat
     3481 tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtaattg gctatctgac
     3541 ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc
     3601 gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc
     3661 cctcaatgcg gtgctcggct gctgcgtggt gagcactttg gtgattcatg ctttagagat
     3721 caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt atctgttatg
     3781 catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg
     3841 gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg cgctgattat
     3901 gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac
     3961 ataatccggc gtttcgacat taatcctagc gatcgtcttt atgatcaagg cggtcgcggt
     4021 catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa
     4081 aaccgccgcc tggcatgcga gaaaagcgca aatgtggcat caacttcact gtcagatgct
     4141 tttgcaccgg cggcaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata
     4201 aaagcagcaa accggcaata atcagcgaaa cacttttaat catctccgcc gcagggtttt
     4261 cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg ttacgtacca
     4321 gtgacatacc gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata
     4381 catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa
     4441 aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gttaaacccg gctacctgaa
     4501 tgggttgcga atcgcgttca gcttatattg tggtcattag caaaatttca agatgtttgc
     4561 gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta ataattaagg
     4621 ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag cagcatatga
     4681 tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat
     4741 atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa
     4801 caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta
     4861 tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga
     4921 tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa
     4981 agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt
     5041 cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg
     5101 tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg
     5161 tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc
     5221 cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaagct
     5281 ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga
     5341 catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga
     5401 attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac gtaccgctga
     5461 actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa
     5521 agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc
     5581 ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg ttcacggcgc
     5641 gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc
     5701 tggtccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg gctcttccat
     5761 catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt
     5821 catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt
     5881 tatggagccg gtcattggcc aggctatgtt cgaatccgtt cacattctga ccaacgcttg
     5941 ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg
     6001 ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa
     6061 cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct
     6121 ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat
     6181 gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt
     6241 agtacaaata aagaaggcac gtcagatgac gtgccttttt tcttgtgagc agtaacttaa
     6301 aaataacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga
     6361 actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg
     6421 ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat
     6481 cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt
     6541 tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa
     6601 atacatcaca atcctcgcac cgatcgtgac ctatttcctg acaatctttg ctggtactgg
     6661 caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa
     6721 accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc
     6781 aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct
     6841 ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtccttcct
     6901 ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga
     6961 agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac
     7021 gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag
     7081 cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa tcctgatcat
     7141 catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacatcct
     7201 caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg
     7261 gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt
     7321 gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta
     7381 ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttctcc
     7441 gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc cgacctatcc
     7501 gacgctggtt gctgcggtac agatggatga cacgggtaca acccgtatcg gtaaattcgt
     7561 cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg
     7621 cttcgtgctg ggtagcttca tgctataatg accaatcgcg gggcgttcac gccccgcttt
     7681 ctttcccgcc gactaacatc cttcccccgt ccgttgtata gtgacctctc tcttgcggtt
     7741 ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg
     7801 tggtgctatg taccgcaccg gatgaagcga cagcccagga tttagccgcc aaagtgctgg
     7861 cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc tattactggg
     7921 aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc gtatctcacc
     7981 agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg gaacttctgg
     8041 ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca tctttacgct
     8101 gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc cgggacgttc
     8161 acattttgtc cccgcggatc aagcctttgc ttttgatttt cagcaaaacc aacatgacct
     8221 gaatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga tccgcattac
     8281 gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc atgaagatga
     8341 gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa ccatcaacca
     8401 ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg ccggtttctg
     8461 ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc
     8521 acagcctgtg tctgttccgc agcaagagca gcacaccgcg caattgccct tttccgcgct
     8581 ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc
     8641 actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca gagcattgtt
     8701 gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg gtctggtagt
     8761 tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc tcattggcct
     8821 cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc tacaactccc
     8881 ctcttcgctg caaacacatc tcacattgat gagcaatcgc caacagggcg gctcacctgg
     8941 cggtgtgttt gttatggggg cgattgccgg actgatctgt tcaccatgca ccaccgcacc
     9001 gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg gcggcggcat
     9061 gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg tctttggtaa
     9121 ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt
     9181 gatcctcgca ctgccgatct tcctgctgga gcgagtgatt ggtgatatat ggggattacg
     9241 cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca gcctacaggc
     9301 caaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat tggttagcgt
     9361 gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc agacgcatct
     9421 caactttaca caaatcaaaa cggtagatga gttaaatcag gcgctcgttg aagccaaagg
     9481 caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaaa agtttgagaa
     9541 atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct tacttcaggc
     9601 caacgtcact gctaacgacg cacaagatgt ggcgctgtta aagcatctta atgtccttgg
     9661 actaccgaca attctctttt ttgacggaca aggccaggag catccacaag cacgcgtcac
     9721 gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac
     9781 acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc
     9841 cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg ttgctgaacg
     9901 tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg caatcctcta
     9961 cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc tgatgcttga
    10021 cgaaacgcaa accgccgaac aaaagctgct ggcacgttat caggcgttat cggagtgcgt
    10081 taaaaacaac cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt atcccgaccc
    10141 tggccaccct attcatcaac tggccaatca gcaaaaaagc gcggcctacg atttcaccca
    10201 cgaactgtta accacgctgg aagttgacga tccggcgatg gtagcaaagc agatggaact
    10261 ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac
    10321 cgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgccagg gtggtgcact
    10381 gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt
    10441 ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc gttgcccgga
    10501 tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg attccgagtc
    10561 cgggcaccaa attcatataa acggacctcc acggaggtcc gtttttcgtt tcaggacgcc
    10621 acgatttaag cgttctgcct ccaaatcaat tctaccgaac tcaaccagat tctccccaca
    10681 tcaccagcat tttgcgggta tatcccaatt cgggaaaatt tgttcctgag cgatagcact
    10741 gactgacgtg aaatgtcgtg cggccccgtg atgttgttga acgtcaaatg acgtcatcag
    10801 gagcgtaacg catccataaa gcacaacacc ggtctgaacg gcgactgatg agattttctg
    10861 aatgagaaca aagagaaatg tatcagtccg cttgctcatg caaagactaa caatccatta
    10921 aaatagtaag cgctccggac aattttccat ggattatttt ctacacattt ttctttggca
    10981 aagatgatga attttgatgg taaggaatat tacttctggt tctcagtaaa atcctttcgt
    11041 aatactatgt aatcaagaag tttatggctg gtaaaaataa cgtcttgcat tcaccaataa
    11101 catgtaaata aacccatcta ttgatggtaa aaataggtca tggcaattat cattgcacca
    11161 ttcccttttc gaatgagttt ctattatgca acaacctgta gttcgcgttg gcgaatggct
    11221 tgttactccg tccataaacc aaattagccg caatgggcgt caacttaccc ttgagccgag
    11281 attaattgat cttctggttt tctttgctca acacagtggc gaagtactta gcagggatga
    11341 acttatcgat aatgtctgga agagaagtat tgtcaccaat cacgttgtga cgcagagtat
    11401 ctcagaacta cgtaagtcat taaaagataa tgatgaagat agtcctgtct atatcgctac
    11461 tgtaccaaag cgcggctata aatgaatggt gccggttatc tggtacagcg aagaagaggg
    11521 agaggaaata atgctatctt cgcctccccc tataccagag gcggttcctg acacaaattc
    11581 tccctcccac agtcttacca ttcaaaacac cgcaacgcca cctgaacaat ccccagttaa
    11641 aagcaaacga ttcactacct tttgggtatg gtttttttcc tgttgtcgtt aggtatctgt
    11701 gtagcactgg tagcgttttc aactcttgat acacgtcttc ctatgagcaa atcgcgtatt
    11761 ttgctcaatc cacgcgatat tgacattaat atggtaaata aaagttgtaa cagctggagt
    11821 tccccgtatc agctctctta cgcgataggc gtgggtgatt tggtggcgac atcacttaac
    11881 accttctcca cctttatggt gcatgacaaa atcaactaca acattgatga accgagcagt
    11941 tccggtaaaa cattatctat tgcctttgtt aatcagcgcc aataccgtgc tcaacaatgc
    12001 tttatgtcgg taaaattagt agacaatgca gatggttcaa ccatgctgga taaacgttat
    12061 gtcatcacta acggtaatca gctggcgatt caaaatgatt tactggagag tttatcaaaa
    12121 gcgttaaacc aaccgtggcc acaacgaatg caggagacgc tccagcaaat tttgcctcat
    12181 cgtggtgcgt tattaactaa tttttatcag gctcatgatt atttactgca tggcgatgac
    12241 aaatcattga atcgtgccag tgaattatta ggtgagattg ttcaatcatc cccagaattt
    12301 acctacgcga gagcagaaaa agcattagtt gatatcgtgc gccattctca acacccttta
    12361 gatgaaaaac aattagcagc actgaacaca gaaatagaca acattgttac actgccggaa
    12421 ttgaacaacc tgtccattat atatcaaata aaagcggtca gtgctctggt aaaaggtaaa
    12481 atagatgagt cttaccaggc gataaatact ggcattgatc ttgaaatgtc ctggctaaat
    12541 tatgtgttgc ttggcaaggt ttatgaaatg aaggggatga accgggaagc agctgatgca
    12601 tatctcaccg cctttaattt acgcccaggg gcaaacaccc tttactggat tgaaaatggt
    12661 attttccaga cttctgttcc ttatgttgta ccttatctcg acaaatttct cgcttcagaa
    12721 taagtaactc cgggttgatt tatgctctgg aatatttgtt gttgagtttt tgtatgttcc
    12781 cattggtata atatgttgcg gcaatttatt tgccgcataa tttttattac ataaatttaa
    12841 ccagagaatg tcatgcaatc cattgtaaac attgaatgtt tatcttttca tgatatcaac
    12901 ttgcgatcct gatgtgttaa taaaaaacct caagttctca cttacagaaa cttttgtgtt
    12961 atttcaccta atctttagga ttaatccttt tttcgtgagt aatcttatcg ccagtttggt
    13021 ctggtcagga aatagttata catcatgacc cggactccaa attcaaaaat gaaattagga
    13081 gaagagcatg agttctgcca agaagatcgg gctatttgcc tgtaccggtg ttgttgccgg
    13141 taatatgatg gggagcggta ttgcattatt acctgcgaac ctagcaagta tcggtggtat
    13201 tgctatctgg ggttggatta tctctattat tggtgcaatg tcgctggcgt atgtatatgc
    13261 ccgactggca acaaaaaacc cgcaacaagg tggcccaatt gcttatgccg gagaaatttc
    13321 tcctgcattt ggttttcaga caggtgttct ttattaccat gctaactgga ttggtaacct
    13381 agcgattggt attaccgctg tatcttatct ttccaccttc ttcccagtat taaatgatcc
    13441 tgttccggcg ggtatcgcct gtattgctat cgtctgggta tttacctttg taaatatgct
    13501 cggcggtacc tgggtaagcc gtttaaccac tattggtctg gtgctggttc ttattcctgt
    13561 ggtgatgact gctattgttg gctggcattg gtttgatgcg gcaacttatg cagctaactg
    13621 gaatactgcg gataccactg atggtcatgc gatcattaaa agtattctgc tctacctgtg
    13681 ggccttcgtg ggtgttgagt ccgcagcagt aagtactggt atggttaaaa acccgaaacg
    13741 taccgttccg ctggcaacca tgctgggtac tggtttagca ggtattgttt acatcgctgc
    13801 gactcaggtg ctttccggta tgtatccgtc ttctgtaatg gcggcttccg gtgctccgtt
    13861 tgcaatcagt gcttcaacta tcctcggtaa ctgggctgcg ccgctggttt ctgcattcac
    13921 cgcctttgcg tgcctgactt ctctgggctc ctggatgatg ttggtaggcc aggcaggtgt
    13981 acgtgccgct aacgacggta acttcccgaa agtttatggt gaagtcgaca gcaacggtat
    14041 tccgaaaaaa ggtctgctgc
//
LOCUS       DAJTXQ010000108        13975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000108 DAJTXQ010000000
VERSION     DAJTXQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.108"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            9..1235
                     /gene="rtcB"
                     /locus_tag="OO515_003089"
     CDS             9..1235
                     /gene="rtcB"
                     /locus_tag="OO515_003089"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6576953.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1239..2255
                     /gene="rtcA"
                     /locus_tag="OO515_003090"
     CDS             1239..2255
                     /gene="rtcA"
                     /locus_tag="OO515_003090"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6576954.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2298..5003)
                     /gene="malT"
                     /locus_tag="OO515_003091"
     CDS             complement(2298..5003)
                     /gene="malT"
                     /locus_tag="OO515_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6576955.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5614..8007
                     /gene="malP"
                     /locus_tag="OO515_003092"
     CDS             5614..8007
                     /gene="malP"
                     /locus_tag="OO515_003092"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6576956.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8017..10101
                     /gene="malQ"
                     /locus_tag="OO515_003093"
     CDS             8017..10101
                     /gene="malQ"
                     /locus_tag="OO515_003093"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6576957.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10146..11462)
                     /gene="gntT"
                     /locus_tag="OO515_003094"
     CDS             complement(10146..11462)
                     /gene="gntT"
                     /locus_tag="OO515_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6576958.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11823..12398)
                     /gene="nfuA"
                     /locus_tag="OO515_003095"
     CDS             complement(11823..12398)
                     /gene="nfuA"
                     /locus_tag="OO515_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6576959.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12457..13140)
                     /gene="gntX"
                     /locus_tag="OO515_003096"
     CDS             complement(12457..13140)
                     /gene="gntX"
                     /locus_tag="OO515_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6576960.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13178..13948
                     /gene="bioH"
                     /locus_tag="OO515_003097"
     CDS             13178..13948
                     /gene="bioH"
                     /locus_tag="OO515_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6576961.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
BASE COUNT         3366 a         3478 c         3926 g         3205 t
ORIGIN      
        1 aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa atgtggacca
       61 aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg aagatgccgt
      121 ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt tccaccattg
      181 gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg gatattggct
      241 gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa aacctggcgg
      301 agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg cgttgtaaac
      361 gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg gctgagctgg
      421 aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact aacaactata
      481 aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt gatgagtcgg
      541 agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc atcgggactt
      601 actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg ccgtcgcgtg
      661 atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga gccgtggcct
      721 gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg gtaacggcat
      781 tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg gaagagatca
      841 actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc tacgtgacgc
      901 gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg tcgatgggag
      961 caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg tgcagccacg
     1021 gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg gaagatcaaa
     1081 ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac gaaatcccga
     1141 tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg gaagttatct
     1201 ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg attgcgctgg
     1261 atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc ctgtcgatga
     1321 taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa ccggggctgt
     1381 tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca acggtggaag
     1441 gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc ggcggcgagt
     1501 accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg gtgctgcccg
     1561 cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc gataacccgt
     1621 cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg aaaataggaa
     1681 ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc ggcgtggtgg
     1741 caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag cgcgggaaca
     1801 ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt gctgagcgtg
     1861 aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat aacctgccgc
     1921 gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat atcactgaac
     1981 gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg cagttagtga
     2041 aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc gccgaccagc
     2101 tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat ccttcatgcc
     2161 atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt ggtttgatag
     2221 aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac cggatccaat
     2281 gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt ctgcgcgtgt
     2341 tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg gatatgcgtc
     2401 ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct gtaaccggag
     2461 tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg gatcagttct
     2521 ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg ggcgaattta
     2581 tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg ttccagttcc
     2641 ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc gccttcgatg
     2701 acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag caacacgcgc
     2761 tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa caggttacgg
     2821 ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc gagaacaatt
     2881 tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg ccattgacct
     2941 tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca gttggcagcg
     3001 gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc gttagagatc
     3061 cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag ctggctacgg
     3121 gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag gcactgaagc
     3181 tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga cgcttcggct
     3241 tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag aaactcatgc
     3301 atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc ctgtgtttcc
     3361 cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat taaactccat
     3421 aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg cattagcgcc
     3481 agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga ggttgctaca
     3541 atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc cagccgttcc
     3601 gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt aaattctgcg
     3661 tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag cagggtgtta
     3721 acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac taactgcgga
     3781 ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag cgacagttcg
     3841 ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat cagcgcatcg
     3901 cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat ccagctttct
     3961 gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca gcgctggcgc
     4021 aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc catccgctgt
     4081 aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc ttcgccagtc
     4141 acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa cagaaaatgg
     4201 cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga aagatggctg
     4261 gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt ctgccgggcg
     4321 gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca aatccaactg
     4381 ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt cgcttcctga
     4441 tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac acgcagattg
     4501 gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt ttctggttga
     4561 tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag atgatagtca
     4621 tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag ctgggcgaag
     4681 agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat cgcacagtga
     4741 ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc ttgctggtta
     4801 tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca ctgggaaatg
     4861 agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa gttgttcgcg
     4921 ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg aaccggacga
     4981 cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga ggaaatatta
     5041 tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc taatgcaagc
     5101 gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat ctttattaac
     5161 aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg ttcatcagat
     5221 tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa aagatgatct
     5281 taataaatct attaagaatg agatggagca cattggatat tttacttgtg aaactgtttc
     5341 actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca acggccattt
     5401 tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct aatcctgtgg
     5461 tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc aactcctccc
     5521 tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact agcgataacg
     5581 ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt taacgataag
     5641 caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc tgcggctgaa
     5701 atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat gctgcgtgct
     5761 cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc aatggagttt
     5821 ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca ggatgtacag
     5881 gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga gatcgacccg
     5941 gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc aatggcaact
     6001 gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt ccgccagtct
     6061 tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa ctacccgtgg
     6121 ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt gacgaaagac
     6181 ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc cgttgtcggc
     6241 tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc gcatccgttt
     6301 gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg catcaatgcg
     6361 gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa aaagctgcgc
     6421 ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg tcgccatcat
     6481 ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct gaacgatacc
     6541 cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca ccagatgagc
     6601 tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca taccctgatg
     6661 ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc gcgccatatg
     6721 cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac ctggccgggc
     6781 gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca tatggcgaac
     6841 ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc ggatctggtg
     6901 gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca taacgtcacc
     6961 aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc ggctctgttg
     7021 gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa tctggaaaaa
     7081 ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc gaataaagtc
     7141 cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca ggcgattttc
     7201 gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct gctgcatatt
     7261 ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc gcgcgtcttc
     7321 ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat ctttgcgatc
     7381 aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt gaaggtggtg
     7441 ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc ggatatctcc
     7501 gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa actggcgctc
     7561 aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc cgagaaagtc
     7621 ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc aattctggcc
     7681 aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc agtattgaaa
     7741 gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca gatgctgcac
     7801 agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc agcctatgtt
     7861 gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac tcgcgcggcg
     7921 atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg cgattatcag
     7981 gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg tctggataat
     8041 gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa accgcagtcg
     8101 attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac cgccacgaaa
     8161 gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa aatgccgatg
     8221 gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg aacacagtac
     8281 aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc ggaaggttat
     8341 cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat tgtcgccccg
     8401 aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg tgcctgcgtt
     8461 cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg cgatctcaaa
     8521 gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa cccgattcat
     8581 gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc tcgccgttgg
     8641 ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag cgaagaggct
     8701 caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg ggatgccgac
     8761 tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat ggcgtggaaa
     8821 ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt tgcagatcaa
     8881 ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca agtgaaagag
     8941 gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt ggattcacca
     9001 gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct ctggttgcag
     9061 tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta tgaaatgccg
     9121 attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga aacctggtgt
     9181 gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat cctcggcccg
     9241 ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc gcgtgcctat
     9301 gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt acgaattgac
     9361 catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc agatcagggc
     9421 gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga aagtaaacgt
     9481 catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat tgtcggtaag
     9541 ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga ccacgagaag
     9601 acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc gacacatgac
     9661 ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa aaccctgggg
     9721 ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact ggcgaagcaa
     9781 gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg gcataaggca
     9841 tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat tgccgacagt
     9901 aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga accggtgaat
     9961 attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc aacgcttgag
    10021 tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag acggcgcaga
    10081 gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc cggcagtcag
    10141 tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac cgagataatg
    10201 gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa gtactctttg
    10261 aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga acccaccgca
    10321 ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc gataccaccc
    10381 gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc agcaatcgac
    10441 caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat gtatttgtcc
    10501 acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat caacagcatc
    10561 atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat tgaacgacca
    10621 cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc cattaccggg
    10681 tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat catttcggca
    10741 atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct gacgccaaag
    10801 ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg gattggctta
    10861 tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt cgggattgcc
    10921 agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt ggcaatcgca
    10981 gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc cgccattggt
    11041 acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac cagcggcagc
    11101 atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc ggttaacacc
    11161 accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc gatacgttgt
    11221 gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat gatcagggca
    11281 aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt atccagcggc
    11341 attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa gccgttcatt
    11401 ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat gactaatggc
    11461 atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg acaaactcta
    11521 attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac taaaactaat
    11581 tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg ggtaacattt
    11641 ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg tcatattatc
    11701 gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct ggcacatgtt
    11761 caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa agaggggata
    11821 acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac acctttcagc
    11881 tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac atcgaccatg
    11941 gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc ggtgatttcc
    12001 atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga ctgcagcata
    12061 tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt ggcgttcggg
    12121 gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc tgcatcttcc
    12181 aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt cagggcagtg
    12241 tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc gttaggcgtg
    12301 ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc cagcagtttg
    12361 gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta atagttgact
    12421 attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac aaaggcacca
    12481 gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg cgacggtact
    12541 tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg gcaattcaag
    12601 acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat gctgggtcgc
    12661 agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc agcgtgataa
    12721 cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc gctgccataa
    12781 cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag cgtgtaagac
    12841 ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg aaaatttaag
    12901 ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca gtctttgcca
    12961 gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg cgggtaatcc
    13021 acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg aacaaatccc
    13081 ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta ctgttagcat
    13141 atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt ggtggcagac
    13201 caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga atgccgaagt
    13261 gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg ttgatctgcc
    13321 cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg ccgaagccgt
    13381 gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg ggctggtggc
    13441 aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg tggcgtcgtc
    13501 accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc tggcgggatt
    13561 tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg cgttacaaac
    13621 catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg ttctggcgtt
    13681 accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa cggttgatct
    13741 ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct atctcgatgg
    13801 tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca gcaaatcata
    13861 tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt tttgtcacct
    13921 gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac agaaa
//
LOCUS       DAJTXQ010000109        13500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000109 DAJTXQ010000000
VERSION     DAJTXQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.109"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..181)
                     /locus_tag="OO515_003098"
                     /pseudo
     CDS             complement(50..181)
                     /locus_tag="OO515_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            182..727
                     /locus_tag="OO515_003099"
                     /pseudo
     CDS             182..727
                     /locus_tag="OO515_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(769..1770)
                     /gene="gap"
                     /locus_tag="OO515_003100"
     CDS             complement(769..1770)
                     /gene="gap"
                     /locus_tag="OO515_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6576962.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            1959..2489
                     /gene="cybB"
                     /locus_tag="OO515_003101"
     CDS             1959..2489
                     /gene="cybB"
                     /locus_tag="OO515_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6576963.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            2734..2907
                     /locus_tag="OO515_003102"
     CDS             2734..2907
                     /locus_tag="OO515_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6576964.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(2979..3128)
                     /gene="hokB"
                     /locus_tag="OO515_003103"
     CDS             complement(2979..3128)
                     /gene="hokB"
                     /locus_tag="OO515_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6576965.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3527..5167
                     /gene="trg"
                     /locus_tag="OO515_003104"
     CDS             3527..5167
                     /gene="trg"
                     /locus_tag="OO515_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6576966.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5205..6128)
                     /locus_tag="OO515_003105"
     CDS             complement(5205..6128)
                     /locus_tag="OO515_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6576967.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6345..7688
                     /locus_tag="OO515_003106"
     CDS             6345..7688
                     /locus_tag="OO515_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6576968.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7913..9568
                     /gene="opgD"
                     /locus_tag="OO515_003107"
                     /gene_synonym="mdoD"
     CDS             7913..9568
                     /gene="opgD"
                     /locus_tag="OO515_003107"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6576969.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            9708..9932
                     /locus_tag="OO515_003108"
     CDS             9708..9932
                     /locus_tag="OO515_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6576970.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9995..10531
                     /gene="rimL"
                     /locus_tag="OO515_003109"
     CDS             9995..10531
                     /gene="rimL"
                     /locus_tag="OO515_003109"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6576971.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10526..11506)
                     /locus_tag="OO515_003110"
     CDS             complement(10526..11506)
                     /locus_tag="OO515_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6576972.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11630..12622
                     /gene="tehA"
                     /locus_tag="OO515_003111"
     CDS             11630..12622
                     /gene="tehA"
                     /locus_tag="OO515_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6576973.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12619..13212
                     /gene="tehB"
                     /locus_tag="OO515_003112"
     CDS             12619..13212
                     /gene="tehB"
                     /locus_tag="OO515_003112"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6576974.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3447 a         3139 c         3412 g         3502 t
ORIGIN      
        1 tcaggagtca tgcccgtttg caaacaagtg gttcacaaac gggcaatttt taacgtaacg
       61 agcgactttc catcgcctcg atgagcaccg catcatgttt cagcgtttgc catgcatgga
      121 tgacttctgc cggaaaatca acgtgaacga taaaatctcg cccgcgggga ccgtagccat
      181 tgatcagttc gtcaatcggc tgggtgaagc gatgcaggcg gttaaatttg gtaaccccgc
      241 tgaacgcaac gacattgcga tggggccgtt gattaacgcc gcggcgctgg aaagggttga
      301 gcaaaaagtg gcgcgcgcag tagaagaagg ggcgagagtg gcgttgggtg gcaaagcggt
      361 agaggggaaa ggatattatt atccgccgac attgctgctg gatgttcgcc aggatatgtc
      421 gattatgcat gaggaaacct ttggcccggt tctgccggtg gtcgcatttg acacgctgga
      481 agaggctatc tcaatggcta atgacagtga ttacggcctg acctcatcaa tctataccca
      541 aaatctgaac gtcgcgatga aagccattaa agggctgaag tttggtgaaa cttacatcaa
      601 ccgtgaaaac ttcgaagcta tgcaaggctt ccacgccgga tggcgtaaat ccggtattgg
      661 cggcgcagat ggtaaacatg gcctgcatga atatctgcag acccaggtgg tttatttaca
      721 gtcgtaatga gtaaaagagg cggaggtttt ttcctccgcc tgtgcgcgtc agagtttagc
      781 gaatttttcg agggtgcgaa taagctgcgt gacgaagcca tattcgttat cgtaccaggc
      841 gaccgttttc accagttgta aatcgcccac ggcggtaatt tccgtttgcg tggcatcaaa
      901 caccgaaccg aaatggctgc caatgatatc ggaagagact atttcttcat cggtataacc
      961 aaatgactcg ttattggtgg ttgcttgttt aagtgcgtta ttcacctctt cggcagtcac
     1021 tttttttccg agaatcgata ccagttcagt gaccgaacct gttttcaccg gcacgcgttg
     1081 cgcatgacct ttcagtttgc cgctcagttc cgggatcacc agaccaatgg cttttgccgc
     1141 ccccgtagtg tggggaatga tattttctgc cgctgcgcgt gaagcacgta aatctttacc
     1201 acgtgggcca tccaccagtg actgggtgcc agtataggca tgaatggtcg tcatcgtgcc
     1261 gacttctatc ccgaaactgt catgcaaggc tttggccatc ggcgcaagac agttagtggt
     1321 gcatgacgcc acggaaacaa tggtgtcgtt gccatccaga gtgtcgtcat tgacgttata
     1381 aacgatagtt ttcatttcac cggcaggggc ggaaatcaac accttcttcg caccagcatc
     1441 aagatgtgcc tgcgatttct cggcggaggt ataaaagcca gtacattcga caatgatttc
     1501 tgcacctttc gctttccacg gaatattttt agcctctttt tcggcgtaaa ccgcgatact
     1561 tttcccatca acgataagtg aatcttccgt aaaatcaacg ctccagggga atggtccgta
     1621 gtttgaatca tgtttcagca ggtaggcgag aatttttggg gaagtgagat cattaatagc
     1681 gacaacgtct atgttgcttt tgacttcaag taatcgacgc aacaccagtc gaccgatacg
     1741 accaaaaccg ttaataccaa ctttactcat ggttttctcc tgtcaggaac attcggatga
     1801 aaattgatcc tttccaggct tagaccagga ttgtgggatg ggcaatctcc attcttacag
     1861 tgaaacgtaa cgtactgaaa acgggtgaac aatatttaat gaaattttga gaaaagcccg
     1921 ttatgttaac ggaaaattat gttaaagcag gaaatgttat ggaaaataaa tactcaaggt
     1981 tacaaatcag cattcactgg ctggtctttt tgctggttat cgcagcgtat tgcgcaatgg
     2041 agtttcgtgg tttcttccca cgtagcgatc ggccactcat caacatgatt catgtttcct
     2101 gcggcatctc aattctcgtg ctgatggtcg ttcgtctttt gttaaggctg aaatacccaa
     2161 ccccgccgat tatacctaag ccaaaaccga tgatgacggg actggcgcat ttgggacatt
     2221 tggtgatgta tctcctgttt attgcgctgc cagtgattgg tttggtgatg atgtataacc
     2281 ggggcaaccc gtggtttgcg tttggtttga cgatgcctta cgcttcagag gccaatttcg
     2341 aacgggttga tagcttaaag tcgtggcatg agacgctggc gaatctggga tattttgtca
     2401 tcgggttgca cgctgcggct gcactggcac accactattt ctggaaggac aacacacttc
     2461 tacgcatgat gccgcgtaaa cgttcctgaa ggatatttca agaaaatgcc tgtactaaaa
     2521 ccgacccgtg gtacaggcga agaatacggg tctacatcgg aagcgcctat tatatttatt
     2581 tgtatgacaa ataaaaccat aatccttgcc catacgtcca tctggcttat ttttaatcaa
     2641 ttcacccgat ctttgatctc atcaacggta tcaaaataaa ccagcgtata acgttcaaat
     2701 cgaacataag attcgataaa ccatggaggt tatatgaaaa aactggcact tattttgttt
     2761 atgggaacgc ttgtttcctt ttatgccgat gccgggcgca aaccctgttc tggttcgaaa
     2821 ggggggatct cacactgtac ggcaggcggc aaatttgtct gtaatgatgg ttctattagt
     2881 gcatcgaaaa aaacatgcac taactgaagt gtaaaagggg tgccatgaga aaatgaatct
     2941 gctagtcaga tgcgcgggga aaatccccgc gcttgccctt acctggacgt gcaggccatg
     3001 agcgcagcaa cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc
     3061 aggagtgtga atgtcagaat cgtaatgcag ataatgagca gacacactac cagagggttg
     3121 tgcttcatag cctttctcct tgccggatgg cgggtaagag gctaagatct gaattgctag
     3181 gttcattcgt tggcctcggt tgatagaaat atcggtcggg gccttcgtct ttctgattcc
     3241 cggttagcct gaaaacagaa agtctcaggc acccgcaggc atcctatgag gtttccttag
     3301 ggacgaaaat aatcacttca cgaaattgcg tgctgttttc cagaattttt cgtcattcgg
     3361 gttagccagt ttagccattc gttactctct tcattccaat agcattaatt ttctctgcaa
     3421 taattgttgt aaaaatgtga cgcaaagagg tttttggtca taagtaatta ccgtcaagtg
     3481 ccgatgactt tctatcagga gtaaacctgg acgagagaca acggtaatga atacaactcc
     3541 ctcacagcga ttaggttttt tgcatcacat caggctggtt ccgttatttg cctgcattct
     3601 tggcggtatc ttagttctat tcgcattaag ttctgctctg gctggctatt tcctctggca
     3661 ggccgatcgc gatcagcgtg atgttactgc ggagattgag attcggaccg ggttagcgaa
     3721 cagttcagat tttttgcgtt cagcccggat caatatgatt caggccgggg ctgcgagttg
     3781 tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca
     3841 gtcgcagcaa gggtatcgtg cttatcagaa tcggccggtg aaaacacctg ctgatgaagc
     3901 cctcgacact gaattaaatc aacgctttca ggcttatatc acgggtatgc aacctatgtt
     3961 gaaatatgcc aaaaatggca tgtttgaagc gattatcaat catgaaagtg agcagatccg
     4021 accgctggat aatgcttata ccgatatttt gaacaaagcc gttaagatac gtagcaccag
     4081 agccaaccaa ctggcggaac tggcccatca gcgcacccgc ttgggtggga tgttcatgat
     4141 tggcgcgttt gtgcttgccc tggtcatgac gctgataaca tttatggtgc tacgtcggat
     4201 cgtcattcgt ccactgcaac atgccgcaca acggattgaa aaaatcgcta gtggcgatct
     4261 gacgatgaag gatgaaccgg cgggtcgtaa tgaaatcggt cgcttaaatc gtcatttaca
     4321 gcaaatgcag cattcactgg ggatgacagt agggactgtt cgacagggtg cggaagagat
     4381 ttatcgtggc accagcgaaa tttcagctgg caatgcggac ctgtcatctc gcaccgaaga
     4441 acaagcggcg gctatcgaac aaactgccgc cagcatggag caactcactg cgacggtgaa
     4501 acagaatgcg gataacgcgc atcatgccag caaactggcg caagaggctt ctattaaagc
     4561 cagcgatggc gggcagacgg tttccggtgt agtaaaaacg atgggcgcta tctccacgag
     4621 ttcgaagaaa atttctgaga tcaccgccgt catcaacagt attgctttcc agacgaatat
     4681 tctggcactg aatgctgccg ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc
     4741 cgttgtcgcc agcgaagtac ggacactcgc aagtcgcagc gctcaggcgg cgaaagagat
     4801 tgaaggcttg atcagtgaat cagtcaggtt aattgacctg gggtcggatg aggtggcaac
     4861 ggccgggaaa accatgagca ctattgttga tgccgtcgcg agtgtcacac atatcatgca
     4921 ggaaatcgcc gccgcctcgg atgaacaaag tagaggcata acgcaggtta gccaggcgat
     4981 ttctgaaatg gataaggtga cgcaacagaa tgcttctctg gtagaagagg cctcagcggc
     5041 ggcggtgtcc cttgaagaac aggcggcacg attaactgag gcggtggatg tattccgtct
     5101 gcacaaacat tctgtgtcgg cagaacctcg cggagcgggt gaaccagtta gtttcgctac
     5161 ggtgtgaaaa tgttcaagga gggatcgaca gatcccttca cctttcagaa cggcattgat
     5221 tttcgaatag cgttaatcat caactggcaa ccagaagaga acgtcgcatc tacgcgggtc
     5281 agtattccaa tcggttcgcc tgcaccatgt cccggaacag gcagggccac cagcgtggca
     5341 tgacgcaggt cgtcttttac agcgccagaa gggacaaacc acacgtaatc gtattcaacc
     5401 gtaagttgac gagatagcga agcagacagc gtttcgatac aacccgaagg aattttacag
     5461 ccctggctct gcactaatgc atctgaatgc tggcgtggcg cagtgccttc tggtgataca
     5521 acgaccggcc attccagcac ccggcttagc gttacgttct cctgaagtag cgggtgatta
     5581 gggcggacaa ccagcttcag cgattcaaga aacagcagtt cgtaattaag cccggtcatc
     5641 agttcaggat ctgacatccg accaatgccg atatcgattt ccccggtttt taaacccgcc
     5701 agaatcatag ggttactcat tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga
     5761 aactgaccta taaccgaagg taatatcccc agtgccgcag taggtagtgc accaaccctg
     5821 acgacatcat tattaagacc ttctttacga tgaagcgact gtccggcagt gttgatggcg
     5881 tcaagaactc tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc
     5941 tgacgaccac gctcaaacaa gcgagcgccc gtcagctgct ccagttcatt caatgtctta
     6001 gagagcgcag gttgactcaa attaagggtt tcagccgcgc gccccaaagt tccttgttgt
     6061 gcgacagcta cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa cagactattt
     6121 ttttccataa gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta
     6181 tgataacttg attgcaaaat attattaaca attaaagcaa ttatgttaca gcaaaatgga
     6241 taatattgat gttttcgcgg cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg
     6301 cggttaccta cactccagat tactgaccac tggaggcaga cactatggcg aacagcatca
     6361 cggcggatga gattcgggaa cagttttcgc aggcaatgtc agccatgtac cagcaagaag
     6421 ttccgcaata tggcacgctg ctggaactgg tagctgatgt gaatctggct gtgctggaaa
     6481 acaatcctca actgcacgaa aaaatggtaa atgcagacga gctggcgcga ctgaatgttg
     6541 aacgtcatgg ggcgattcgc gttgggactg cacaagagct tgctactctt cggcggatgt
     6601 ttgccattat ggggatgtac ccggtgagct attacgatct ctcgcaggca ggggtgccgg
     6661 tacattcgac agcatttcgg cccattgatg atgcttctct ggcgcgtaat cccttccgcg
     6721 tttttacctc cttactccgc cttgagctta tcgagaacga aattttgcgc cagaaagcgg
     6781 cggagattct gcgtcagcgc gatatcttca ccccacgttg tcgacaactg ttagaggaat
     6841 atgagcagca gggcggtttt aacgaaacac aggcacagga gtttgtgcag gaagccctgg
     6901 aaacgtttcg ctggcaccag tcagcaacgg tagatgaaga aacctatcgc gccttgcaca
     6961 acgaacatcg gttgattgct gatgtggtct gttttcctgg atgccatatc aaccacctga
     7021 cgccacgtac gctggatatt gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac
     7081 ccaaaattct catcgagggg ccgccgcgcc gcgaggtacc gattttacta cgccagacca
     7141 gctttaaagc actggaagag acggtgttgt ttgcggggca gaaacagggc acgcataccg
     7201 cgcgctttgg tgaaattgag cagcgtggcg tggcattaac gccgaaaggg cgacaactgt
     7261 atgatgatct tctgcgtaac gctggaaccg ggcaggataa tctcactcac caaatgcatt
     7321 tacaggaaac cttccgcact tttcctgaca gtgagttttt aatgcgtcag caagggttgg
     7381 catggttccg gtaccgtctg acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg
     7441 gagacgatcc acagccctta attgaacgtg gttgggtagt ggcgcaaccc atcacctatg
     7501 aagatttctt gcccgttagc gcggcgggga tcttccagtc aaatctgggt aatgaaacgc
     7561 agacacgcag tcacggtaat gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg
     7621 ttttggatga gttccagctt taccaggaag cggaagaacg cagtaaacgt cgctgtggtt
     7681 tgctttaaat tctgaccatc cgcctttgca aaaatttgcc tgattttaca aacgaatcag
     7741 gctcatccca tcgacataaa aaaaatgccg atttatgcat attctctcag ttcaacaatt
     7801 ggattattaa taaatattgt ctagagtgag cggtcataaa taagcacttt cttgccgctg
     7861 aaaacgacca gcgcgggacc attcacaaca ccagaaggac tcactttcag gtatggatcg
     7921 tagacgattt attaaaggtt caatggctat ggccgccgtg tgcggtacca gcggcattgc
     7981 ttctcttttt tctcaggcgg cattcgcggc agattctgat attgccgacg ggcaaaccca
     8041 gcgttttgac ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg
     8101 tggtgcgcct cgtccgttac ctgacacgct ggcgacaatg acgccgcagg cttataacag
     8161 tattcaatac gacgccgaaa aatcgctctg gcataacgtt gagaaccgtc aactggacgc
     8221 tcagttcttc catatgggaa tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc
     8281 agcaacacat ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat acaacgatgc
     8341 aggtgttgat accaaacaat tagaagggca aagcgatctc ggctttgccg gttttcgcgt
     8401 gtttaaagcc cccgaactgg cgcgccgtga tgtagtatca tttctcggcg cgagttattt
     8461 ccgcgccgtt gatgatacat atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac
     8521 ttacaccgac agtaaagaag agttccccga ctttaccgcc ttctggtttg atacggtaaa
     8581 accgggggca actaccttta ccgtttatgc gttgctcgat agcgccagta ttactggtgc
     8641 ctataagttc actatccatt gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct
     8701 gtatgcgctc aaagacatta aacagctggg cattgcgccg atgaccagta tgttcagctg
     8761 cggtactaat gaacgtcgga tgtgcgacac cattcatccg caaattcatg actctgatcg
     8821 tctgtccatg tggcggggca acggcgagtg gatttgccgt ccgctgaaca atccgcaaaa
     8881 attgcagttc aatgcttaca ccgacaacaa cccgaaaggg tttggtttat tgcaactgga
     8941 tcgtgacttc tcccattatc aggacattat gggctggtat aacaaacgcc caagtttgtg
     9001 ggtggaaccg cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa tcccaacaac
     9061 gggcgaaacg ctggataaca ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg
     9121 tgatgagttt gcattccagt atcgtctgta ctggagtgcg caaccgcctg ttcattgccc
     9181 attagcgcgc gttatggcga cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc
     9241 aggtgaacac tatcccgaaa aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga
     9301 tctgaaagct gccgcgccaa aaggcattga gccggtgatt acgctttcca gtggggaagc
     9361 gaagcaaatc gaaattctct atattgaacc catcgatggt tatcgtattc agtttgactg
     9421 gtatccgact tcggactcta ctgatccggt cgatatgcgg atgtatctac gttgtcaggg
     9481 ggacgctatc agtgaaacat ggctgtatca gtatttcccg ccagcgccgg ataaacgtca
     9541 gtatgttgac gaccgcgtga tgagttaatc gttttttctt cggcaccttc ttcgggaggt
     9601 gccgtctggt taaacacgat cccgctcgca tttttcccta agttaaatga gtaatctgat
     9661 ggtgtgtatt tcagatacac cttgtcagcc actaacaggg agtgcgtatg tttccagaat
     9721 accgagattt aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata
     9781 aacacaataa acttgatcat gaaattgcca gaaaggaagg ttccgacggt cgagggtaca
     9841 atgcggaagt ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa
     9901 tcctgcagca ggagagcgtc aaagaggtgt aaactttcct aagccgccag ccaggcggct
     9961 tttttaacaa cagcatggat tgactggaga taagatgact gaaacgataa aagtaagcga
    10021 atcacttgaa ttacatgctg ttgcggaaag tcacgtcaca cctctttatc agttaatctg
    10081 taaaaataaa acctggttac agcagtcgct aaactggccg cagttcgttc agagtgaaga
    10141 ggacacgcga aaaacggtgc agggtaatgt gatgttgcat caacgcggct atgccaaaat
    10201 gttcatgatt ttcaaagaag atgaacttat cggcgttatc tcgtttaatc gtattgaacc
    10261 actgaataaa actgctgaaa tcggctactg gctggacgaa tctcatcagg ggcaggggat
    10321 catttctcag gcgctgcagg cattgattca tcattacgct cagtctggtg aacttagacg
    10381 cttcgtgatc aaatgtcggg tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg
    10441 ttttatcctt gaaggttgcc tgaaacaggc tgagttcctg aatgatgcct atgatgatgt
    10501 gaacttatac gcgcgtatta tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc
    10561 gctcctcgcc gttaatgact tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt
    10621 caacgacggc gtggtcggtt aactccacat ggttttcaat tatcaccgca cctgaaatac
    10681 ggctctcacc ttgtattaca acgtgctcgt cgagcagaat cggtccacca cgtaccacgg
    10741 catttccgcc aatcaagaca tgatgtttta acacacaatt accttccaca atggcatatt
    10801 ccgccacctg cgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg ccagctttca
    10861 cctgcgcgtg accgtagact tttgcgcaat cgcatagcca gacgttgttc tcttcattac
    10921 cttcaacact ggcaaaatca aaaacttcgg cgcgatgttc aataaaagca taccggacga
    10981 cggcatcgcc ataaatttgt gcctggtgga caatacgcga ggcgctaacc cttgcgcgat
    11041 cataaatttg gagcaggagt tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag
    11101 aatgttgatc aattaaggcg tgaccaaata ttctacattg cccgtaaact aatgagtcac
    11161 gaatggtgac actgtcactt atatatgcac cctggctgat ttcactatta tcgatccaga
    11221 cgttatccgt tgcgtaaacc tctccccaca gcacgctggt gccggtgata cgcgtattac
    11281 cagaaatcac cgttccgccg aacgcaatag cattctggtc gtaaatccag caatttcctt
    11341 cctgtgctaa caccgtttcg cgatcgatcc agccgccagc ggttcctgct atcacatcgt
    11401 taaaatcgct aatggcgata atctgccgta ataacacact ttttttagtg ccatcttcct
    11461 gataactaaa ggctcgctgt tcttcactta gacgatattt gcgcatagcg ttttctcaca
    11521 ggtgacttac tataaccgta gcaaattctg cggctctggc tatgctcgag aaaattccat
    11581 aaaatgcatt tcaaatatac tttataaatt aaacaaaatg agtaagaaga tgcagagcga
    11641 taaagtgctc aatttgccgg caggctactt tggtattgtg ttggggacga tagggatggg
    11701 atttgcctgg cgctatgcca gccaggtttg gcaggtcagc cactggttag gggatgggct
    11761 ggtgattctg gcgatgatca tctggggatt attaactagc gcatttatta cccgactcat
    11821 acgctttccg catagcgtgc tggcggaagt tcgccatcca gtgctgagca gttttgtgag
    11881 tttgtttccg gcaacgacga tgctggtggc gattggtttt gttccgtggt ttcgcccact
    11941 ggcggtgtgc ctgttcagtt ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac
    12001 tgcgggatta tggcgcggat ctcaccctga agaagctacc acgcctggac tgtatctgcc
    12061 gacagttgcc aacaacttta tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc
    12121 cggtctggtg tttttaggcg caggcgtttt ctcatggcta agtcttgaac cggtgatctt
    12181 gcagcgtctg cgcagttcgg gagaattacc cacggcactg cggacatcac tcggcattca
    12241 gctcgctcct gcgctggtgg cctgtagtgc ctggttgagc gtcaacggcg gcgagggtga
    12301 tacgctggcg aaaatgcttt ttggttatgg actgctgcaa ctgctgttta tgctacgtct
    12361 gatgccatgg tatctctccc agccatttaa tgcttcattc tggagtttct cgttcggcgt
    12421 atctgcactg gcaaccaccg gtttgcatct ggggagtggc agcgaaaatg gatttttcca
    12481 tacgctggcg gtaccgctgt ttatctttac caattttatt attgcaatac tgctcatccg
    12541 tacttttgcg cttctgatgc agggaaaatt gttagtcaga accgagcgcg ccgttttaat
    12601 gaaagcagag gacaaagaat gatcattcgt gacgaaaact attttactga taaatatgaa
    12661 ttaacccgca cacactctga agtactggaa gcggtgaaag tggttaaacc gggtaaaacg
    12721 ctggatctgg gctgtggcaa tggtcgtaac agtctttacc tggcagccaa tggttatgat
    12781 gttgacgcat gggataaaaa tgccatgagt atcgccaatg tagagcgcat taaatccatt
    12841 gaaaatctgg ataatttaca cacccgagtc gttgatctga ataacctcac atttgataga
    12901 cagtacgatt ttattctttc gactgtggta ctgatgttcc ttgaggctaa aaccatccca
    12961 gggctgattg ccaatatgca acgttgcact aaacctggtg gttacaacct gattgtggcg
    13021 gcgatggata ccgctgatta tccatgtacc gtcggcttcc cgtttgcctt caaagaggga
    13081 gaattacgtc gatattacga aggctgggag agggtgaaat acaatgaaga cgtcggcgag
    13141 ctgcaccgca ccgacgccaa cggtaatcgt attaaactgc gtttcgccac gatgctggca
    13201 cgtaaaaaat gacccgctaa gaataaggcg cgtgaaaatt ccccacgctg agatgattta
    13261 ctgttcttct tttcggtaag catatttttt atcgaaggga tgtgaaatta atcacagtag
    13321 tcgaagtttt ttagcagctt aacttactga aatttaacta ctgatgattg acttagcccc
    13381 tttttcggca ttgactatgt cgtctgaaaa ggggctgaaa aatttatttt caccaacact
    13441 ttttttgcca caacacgaag cggcgctttt tgctataact taaaaagtaa tataatcatc
//
LOCUS       DAJTXQ010000110        13299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000110 DAJTXQ010000000
VERSION     DAJTXQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.110"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OO515_003113"
     CDS             complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OO515_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6576975.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNI"
     gene            1102..1665
                     /gene="ahpC"
                     /locus_tag="OO515_003114"
     CDS             1102..1665
                     /gene="ahpC"
                     /locus_tag="OO515_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6576976.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1836..3401
                     /gene="ahpF"
                     /locus_tag="OO515_003115"
     CDS             1836..3401
                     /gene="ahpF"
                     /locus_tag="OO515_003115"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6576977.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OO515_003116"
     CDS             complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OO515_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6576978.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4171..5409
                     /locus_tag="OO515_003117"
     CDS             4171..5409
                     /locus_tag="OO515_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6576979.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OO515_003118"
     CDS             complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OO515_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6576980.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OO515_003119"
     CDS             complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OO515_003119"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6576981.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OO515_003120"
     CDS             complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OO515_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6576982.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OO515_003121"
     CDS             complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OO515_003121"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6576983.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OO515_003122"
     CDS             complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OO515_003122"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6576984.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OO515_003123"
     CDS             complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OO515_003123"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6576985.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OO515_003124"
     CDS             complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OO515_003124"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6576986.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OO515_003125"
     CDS             complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OO515_003125"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6576987.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12881..>13299)
                     /locus_tag="OO515_003126"
     CDS             complement(12881..>13299)
                     /locus_tag="OO515_003126"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6576988.1"
                     /translation="IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRF
                     VGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKN
                     DLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3261 a         3372 c         3547 g         3119 t
ORIGIN      
        1 tgatattaag cgttttctga tcgggcaacc ccacggcctg ttgcagcgta ttttccttac
       61 tcatgtaata gatagccggc gtgacatttg cccccagatc gtccatcagt ttctcattgt
      121 cacttaacac tttcatttgc tctgtactta cgtttgcagg cacgtttagc ttaagcttgc
      181 caccagaggc ttcatattgt tgccaggttt ttgcgggatc tttggaggca agaattgccg
      241 ctgctgtcgc cgggctttct ggcttgataa ccccaaccaa caatgttctt aattgcactt
      301 tgccagaatc tacccacggg cgcgcctgct gccagaactg tttacaatat gggcagaacg
      361 gatcggcgaa gacgtagaca atgaccggcg catctttttt accgtcgagg atccagtggg
      421 attgttccat ccgttgccac atttcgcgtc cggctggtgc gtaaatttct ttttcgataa
      481 gtgtgttact caggttttca cctttctcgt tgtacatgta accagagata gcgtgcttac
      541 catcgggagt caggtagatg gtgacgccca tatcctgata ctttccgaga taacctttca
      601 ttcctccggg ggcatcgaac gttttgatga ttgtaatgcc ctgtttttca atcgctttta
      661 ctggggaagg aagttcctct gcgaaggcga ttgcaggaag cagagccagt aaaagtatct
      721 tttttaacat taatttgtcc ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta
      781 tcactgtcat aggtaagagc ttagatcagg tgattgccct ttgtttatga gggtgttgta
      841 atccatgtcg ttgttgcatt tgtaagggca acacctcagc ctgcaggcag gcgctgaaga
      901 taccaaaggg tagttcagat tacacggtca cctggaaagg gggccatttt actttttatc
      961 gccgctggcg gtgcaaagtt cacaaagttg tcttacgaag gttgtaaggt aaaacttatc
     1021 gatttgataa tggaaacgca ttagccgaat cggcaaaaat tggttacctt acatctcatc
     1081 gaaaacacgg aggaagtata gatgtccttg attaacacca aaattaaacc ttttaaaaac
     1141 caggcattca aaaacggcga attcatcgaa atcaccgaaa aagataccga aggccgctgg
     1201 agcgtcttct tcttctaccc ggctgacttt actttcgtat gcccgaccga actgggtgac
     1261 gttgctgacc actacgaaga actgcagaaa ctgggcgtag acgtatacgc agtatctacc
     1321 gatactcact tcacccacaa agcatggcac agcagctctg aaaccatcgc taaaatcaaa
     1381 tatgcgatga tcggcgaccc gactggcgcc ctgacccgta acttcgacaa catgcgtgaa
     1441 gatgaaggtc tggctgaccg tgcgaccttc gttgttgacc cgcagggtat catccaggca
     1501 atcgaagtta ccgctgaagg cattggccgt gacgcgtctg atctgctgcg taaaatcaaa
     1561 gcagctcagt acgtagcttc tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt
     1621 gaagcaactc tggctccgtc tctggacctg gttggtaaaa tctaaatttc cttcgtcttt
     1681 cgcgctacca gctccggcgc gaaatacttc ggaaattcac ctaattcttc gggtgctgcg
     1741 gcacccgatt ttcttccccg caccatgatg caagctgcat ccaggtagcc gtagaggccg
     1801 cttgcatgat gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc
     1861 aactcaaggc ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg
     1921 acagcgctaa atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca
     1981 aagtcacctt taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca
     2041 acccaggttc caaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct
     2101 cgctggtact ggcgttgctg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc
     2161 tggagcagat tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt
     2221 gccataactg cccggacgtg gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca
     2281 tcaagcacac tgcaattgac ggcggcacct tccagaacga aatcaccgat cgcaacgtga
     2341 tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg tcagggccgc atgacgttga
     2401 ctgaaatcgt tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca
     2461 agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa
     2521 tttactccgc acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga
     2581 tcctcgatac cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc
     2641 tggcaggcgc actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga
     2701 gcgccagcaa actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt
     2761 ctggcgcggt actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca
     2821 tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg
     2881 acggcccgct gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg
     2941 aagcggcaat tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag
     3001 aaatgaaagc cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta
     3061 ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat
     3121 atcgagatcg tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga
     3181 ttggtctgct gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg
     3241 agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact
     3301 gtacgacggt tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctt
     3361 tgagtgcttt tgactacctg attcgcacca aaactgcata agaagaagta agattcacct
     3421 gcaattgctt agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg
     3481 ccgggatgtt ccaggcaggg cggcataaat acttttatct gttagcgaat gaccagtaca
     3541 ggcagattgg cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc
     3601 gatgggttgc gagaaccgat aactacaaca tcagccccca gttcttctgc caactcattg
     3661 acttcatccc gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg
     3721 aagtggctga ccatcgtttg cagacgttct tctgcttcat gttgcagatg ctcttcaaaa
     3781 cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga
     3841 agtagatgaa taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg
     3901 ctcaattcca tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct
     3961 ccctgttaat catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc
     4021 tggttctttt tccttacgaa cggtttctgt gattaatata ttctcactga acaccaggaa
     4081 ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg
     4141 actctttttt gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat
     4201 cacgttcagg tagaaaatgt tcccgatccg ggcattgaac aggctgatga tattattctg
     4261 cgtattacgg caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc
     4321 caggttaaac atggcgatat tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga
     4381 aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt
     4441 ggcgactgtt ttttctgtcg aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt
     4501 aaaggcgctg cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt
     4561 cacctgtatg gtggcgttcc tggtggacag gcggaatatg tccgcgtccc taaagggaat
     4621 gtggggccgt ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat
     4681 attctgccaa cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt
     4741 gcagtctatg gtgccggtcc agtgggattg ttgacaatcg cctgtgcacg gttgctcggt
     4801 gcggaacaga tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc
     4861 tacggcgcga tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa
     4921 caaacggcag gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa
     4981 ggcagcacca cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg
     5041 ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac
     5101 gctggattta ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa
     5161 atgggacaga cccacgttca cgcatggctg ggtgaattat taccgttaat tgagaaagga
     5221 ttactgaaac cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga
     5281 tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg tgattctggt acccggtgca
     5341 caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga
     5401 acaatatgat cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc
     5461 cataatcaga aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga
     5521 aacggaataa ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc
     5581 gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt atcgcttgtg cagatgggat
     5641 taaagcaggt agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg
     5701 gttgcaacgc cgcccggaag ttcccagtga atggaatcgc caacgcgcag ccccagcagt
     5761 gcggcaccta ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga
     5821 tacaccagcg tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg
     5881 ttcattgtca ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac
     5941 tctgcgttta acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata
     6001 tcgatgcgtt cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca
     6061 tgtcgtcggt actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag
     6121 gcggcgaggg tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg
     6181 cgtcacattc agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcacggt
     6241 ttatggaaga cgaattagcg tgttttgtaa atcagatgat taataaccca ctttatcaat
     6301 cacaaagttt ttgccacagt tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag
     6361 cggagcgttg atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt
     6421 accctggcac gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc
     6481 ggcgtcaaag tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc
     6541 ttcgctctct ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc
     6601 aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc
     6661 agctcctggc atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg
     6721 cgatgaacac atccggctgg cgtgcgcttc tggtagattc gggattgggc gagtagcgca
     6781 accgaagcgc atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc
     6841 tggccacaga ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg
     6901 acattcatct cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca
     6961 ggagagggcc aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc
     7021 gttggcagaa gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc
     7081 tttcataatg ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa
     7141 gcccgccagg tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat
     7201 tagttccaca tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc
     7261 ccgaagattg cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata
     7321 atcaccccag gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc
     7381 agcaggatac acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata
     7441 accgacagca tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag
     7501 aacaccagaa ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc
     7561 atggtaccgg cgaaccagtc aataaagcca gaacgagtca ggccgttagc catcacaacc
     7621 agagttgcca ggttgaccag cgtgttccat gcgctgttat agcgggtaat atctttccaa
     7681 ggcacaacgt gcagggccag cattagcgaa actgccagca gaccaactgc agtagcatta
     7741 atgacttcac tgccaaaaac ccacaaacct aagctgagca atacaaggcc aatcagtgtc
     7801 cactctctgc gtgtcagcgc acccatggtt ttcagttcat cacccgccca ggttgccact
     7861 tcttcactgt gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata
     7921 agcaagataa ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata
     7981 ccggcaattt tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac
     8041 atggacgaac tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga
     8101 cgcgcggacg gatcgttggg gaatgattta aacagcggcg gcaggttttt aatgaccgga
     8161 aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg
     8221 ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa
     8281 agggcaatgc gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca
     8341 aataccagcc ataccgtggt gctggaaaaa ccagccagac cccatttcag cgcctgtttt
     8401 tgcgcattaa acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca
     8461 ataacgcaaa tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc
     8521 atgccgacaa tcattgccac aaacacagcg aagtaatgcc atgcctgtgg tggcataccg
     8581 tcggggacag ggataagaaa catgacaccc atcaccacca gtggggccaa tagtttccat
     8641 atattatctt ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca
     8701 agtgcttaaa taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc
     8761 cgcctggact gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat
     8821 cggcgggggt tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc
     8881 gcaggccccc ctcgccaccg cgcgatgcaa cgttggtatc gccgttgatc gccatcagta
     8941 ggagcaaggt atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt
     9001 aatgcggcaa ggcgtgattg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc
     9061 cggtaaggcc aagctgctgg tacaaccgtt gacctgccgt cagttgtgaa ttattggtac
     9121 gcagttctcg atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg
     9181 gcgttaccgg ttggttgagt tgaagcaaac ggccaattgc cgcacatagt agccctaaag
     9241 aaaaaatgct gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc
     9301 aagccatacc aattgggcgt aatccgtgga gtaccgcttc tggtgctatt tccgcactac
     9361 aggcaccaaa ttcaatgaaa cggggtagcc agccctgaat cgccagcgcg ctgcggtgga
     9421 aatcttccag cgccatatct ttatgtgcac cgcagttaat ccgatccacg aggcctggtt
     9481 tcggtgacag attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga
     9541 tcaatgacgt cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc
     9601 agtgcctcca tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg
     9661 gctgcgcttt gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag
     9721 agaatttcgc cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta
     9781 tgttcaagct caatggtggc gagcttgagg tcgcgagccg gggcggcaat gctcaacatg
     9841 ccctccggcc cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgtttt
     9901 gcggctaagg cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg
     9961 tctttaatcg gcccaggcgc aaccacggta aaggagacca gtggaacagg atggcgcttg
    10021 agccagacgt gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac
    10081 accgcatggt ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc
    10141 acaacatcga tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat
    10201 tcaatcggct gtggttcacc ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa
    10261 accactttaa tgcccgcttc ctgcagacgt tctgccagtt ccggacgtgc cgggttaact
    10321 gcgataccgt ggtctgtgac cagaatatcg acactggagc ctggggtgat gcaggtcagc
    10381 acgttatcca ccagagtcgg aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc
    10441 gcagaggcaa tcgcggtatc gcagtgacca ccggaagcac cacgcagtac gccgtcagag
    10501 ccggtcagca cgttaacgtt gaactgggtg tcaatttcca gcgcgctcag taccaccacg
    10561 tcgagacgat caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt
    10621 tcgatgtgat tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca
    10681 tccagcagtt tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg
    10741 ccgagggcga agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt
    10801 accgccagcg atgcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca
    10861 gagttgacaa tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc
    10921 atacgggtcg cgccagcgcc gatttttgca gcatcgccaa cgcggtcaac tttgacgatc
    10981 aaatcaacct gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg
    11041 gtaagcatca caacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg
    11101 cagcaggctt taccggtgta accgttggca ttaccgaatt catcacagga cgggacgccg
    11161 aggaaagcca cgtcgatatt cagttcgccg ctctgtacca gatgcacacg tccgccgtga
    11221 gagtggattt gcaccggttc cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg
    11281 cgcaggccgg aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagtggc
    11341 gcatggcaat cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttagcg
    11401 atgacgtcca tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa
    11461 accgtcatgc cgtcctgtaa accagagcga cgaatcgctt cttcctggtt ggcgcacagt
    11521 ttgcgatcgc gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca
    11581 agatcgcatt cagcgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca
    11641 attttctgcg tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga
    11701 gagcaccaga cgggcacgat cgataaccgg accgtccacc atcttgccgt tcagggaaac
    11761 cacgccgagg ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc
    11821 cacttctttc tgggtcggtg cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat
    11881 cagcgatttg ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc
    11941 ttcgttgtta gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc
    12001 ctgcaaaatg gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg
    12061 caggttgcgc acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg
    12121 agcgatttcc actgcgcggg taatccccag cggagattca atcgccgcca gcaggccggt
    12181 gctgccgggt tcacgaccac aggctttttc gatacgcagg atctcttttt cgatatccag
    12241 aacatcctga gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac
    12301 ggcttccagg tcgttgacac cccattcgga atccagcgcg ttgacacgca caatggtttc
    12361 aatatcgcga tacagcggat gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt
    12421 ttcacgcaat gctacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa
    12481 ggagttgctg accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg
    12541 agttttacgt tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg
    12601 ataccgctgg cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg
    12661 cctttgtcat cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga
    12721 atggtggtgc gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg
    12781 atatcctgcg tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct
    12841 gcaacggcgg gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac
    12901 gagctgccgc gtcctggcgg gagtgctcaa gcaggttctg caaataatgc agcgtgactg
    12961 cagggaccag cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg
    13021 aagcggatat cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg
    13081 gtgcggagat agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa
    13141 cgcgacaaaa gggttcagta ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt
    13201 actgacggaa aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt
    13261 taatgaagta gcaagggaac gtagcgcggg agatgatgt
//
LOCUS       DAJTXQ010000111        13259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000111 DAJTXQ010000000
VERSION     DAJTXQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.111"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(892..1983)
                     /gene="ychF"
                     /locus_tag="OO515_003127"
     CDS             complement(892..1983)
                     /gene="ychF"
                     /locus_tag="OO515_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6576989.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2100..2684)
                     /gene="pth"
                     /locus_tag="OO515_003128"
     CDS             complement(2100..2684)
                     /gene="pth"
                     /locus_tag="OO515_003128"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6576990.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2961..3239
                     /gene="ychH"
                     /locus_tag="OO515_003129"
     CDS             2961..3239
                     /gene="ychH"
                     /locus_tag="OO515_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6576991.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3294..4973)
                     /gene="dauA"
                     /locus_tag="OO515_003130"
     CDS             complement(3294..4973)
                     /gene="dauA"
                     /locus_tag="OO515_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6576992.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5097..6044)
                     /gene="prs"
                     /locus_tag="OO515_003131"
     CDS             complement(5097..6044)
                     /gene="prs"
                     /locus_tag="OO515_003131"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6576993.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6195..7046)
                     /gene="ispE"
                     /locus_tag="OO515_003132"
     CDS             complement(6195..7046)
                     /gene="ispE"
                     /locus_tag="OO515_003132"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6576994.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7046..7669)
                     /gene="lolB"
                     /locus_tag="OO515_003133"
     CDS             complement(7046..7669)
                     /gene="lolB"
                     /locus_tag="OO515_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6576995.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7883..9139
                     /gene="hemA"
                     /locus_tag="OO515_003134"
     CDS             7883..9139
                     /gene="hemA"
                     /locus_tag="OO515_003134"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6576996.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9181..10263
                     /gene="prfA"
                     /locus_tag="OO515_003135"
     CDS             9181..10263
                     /gene="prfA"
                     /locus_tag="OO515_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6576997.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10263..11096
                     /gene="prmC"
                     /locus_tag="OO515_003136"
     CDS             10263..11096
                     /gene="prmC"
                     /locus_tag="OO515_003136"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6576998.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11093..11485
                     /locus_tag="OO515_003137"
     CDS             11093..11485
                     /locus_tag="OO515_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6576999.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11489..12297
                     /locus_tag="OO515_003138"
                     /pseudo
     CDS             11489..12297
                     /locus_tag="OO515_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12333..13187
                     /gene="kdsA"
                     /locus_tag="OO515_003139"
     CDS             12333..13187
                     /gene="kdsA"
                     /locus_tag="OO515_003139"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6577000.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3344 a         3286 c         3394 g         3235 t
ORIGIN      
        1 tcagcgagac gatctgcttt ggtattccca ttgtgttgtt tgatgcccat accactccta
       61 tatagtaccc agactatggg tctatggatt ttgtcatttt gtttaaatca atgaataata
      121 tcctcttatc atcatgatat ttgtatggtg caatcatgat gagcttctca gagaataatt
      181 ctctattacc gggcaaaatc aggaacacag gaattaatat atatttaaca tcccccttac
      241 aaaggtgagt ttatatcgag ttattcccta agttgtcgtt acttattcca attcgttact
      301 ataaatatgg caaaaatatt acaacaaaca gcggttaaaa aatttggtga agattttttg
      361 taataatcct aattagttat gggaatttcg cttgatgtat ctgcataaca aaaacaatat
      421 gcgccatttt tgcattacta ataagaaaaa gcaaaaaaca tcgatattca tttttggtca
      481 atgggttttc tggcctatcg ttgttattgt gctactggtt atcattttca tcttgccagg
      541 atcgcacgta atcacgttat tcggggactt tatctgcggg atattaccct tcaggtaagt
      601 ccttttctcg ccagcatatt ggagcgtgat caatattgat cagctgtgaa gggggtgatg
      661 tgtagttgaa caaaaactta cgttatatca ctactaatga gatatccgtc atctgacact
      721 gatgtcttta attattctcg tggttcactg taggcaataa gcacaaaagt gtaggatgtt
      781 acaagaatga ttaggactcg gtgaaatgaa aaatccacgc aattgcgtgg attttatata
      841 cttttgcgct cttcatgaga tttagcgaaa cctcatgaga caataaatta attagacgtt
      901 gaaaaggaag ttcatcacat cgccatcttt aacgatgtaa tctttacctt ctgcacgcat
      961 tttgcctgct tctttcgcgc cttgttcacc tttgtaagtg atgaaatctt caaacgagat
     1021 ggtttgtgca cggataaagc ctttttcaaa atcagtatgg attttgcccg ctgcctgcgg
     1081 cgcggttgct ccaaccggaa tggtccatgc acgcacttct ttcaccccag cggtgaagta
     1141 agtttgcagg ttcagcagct tataaccgac acggatcaca cggttcaggc ccggttcttc
     1201 cagcccaagc tcctgcataa actcgtcacg ttcttcgtcg tccagttcgg caatgtctgc
     1261 ttcaacagca gcacaaaccg gaaccacaac agaaccttct ttcgccgcga tttcacgcac
     1321 ctggtcaaga tatgggttgt tttcaaaacc gtcttcgttg acgttggcga tgtacattgt
     1381 tggtttcagc gtcaggaagc tcaggtaacg aatagccgct ttctcttcag cgcttaaatc
     1441 cagcgcgcgc agcatacctg cgttttccag ctggggcagg catttttcta ggaccgccag
     1501 ctcagctttc gcgtctttat cgccaccttt ggctttcttc tgtacgcgat gaatcgcacg
     1561 ttcgcaggtg tcgaggtctg ccagcgccag ttcggtgttg ataacttcaa tatcgtcagc
     1621 cgggttaact ttgccggaaa cgtgaatgat gttgtcattt tcaaagcagc gaacaacgtg
     1681 accgatcgct tcggtttcac ggatgttggt caggaactgg ttacccagac cttcgccttt
     1741 cgatgcgcct tttaccagac cggcgatatc gacaaattcc atggtcgtgg gaagcgtacg
     1801 ttgcggtttt acgatttcag ccagttgatc caggcgaggg tcaggcattg gtacgacgcc
     1861 tgtgttcggc tcaatggtgc agaatggaaa gttggccgct tcaataccgg ctttggtcag
     1921 cgcgttgaac agggtagatt tcccgacgtt gggcaaaccg acgataccgc atttgaatcc
     1981 catgatttaa atcaccttaa tatcttaata atcaacctgt tattgctaac agattgcaga
     2041 aatggaaata actttgccta ttatacacgg cactcggcaa aaatgccgca gacaacgact
     2101 tattgcgctt taaaggcgtg caatcggttc gttgctttgg tcaagccatc tgtaaaccac
     2161 atttcagtac aacgcgccgc ttcgtcgatg gcttcatcaa ttaacttctg ttcactaaca
     2221 ggcggtttgc ctaacacaaa accgacaact ttatttttat cgccaggatg accgattccg
     2281 atgcgtaaac ggtgaaagtt agggttatta cccaatttac tgatgatgtc tttcagtcca
     2341 ttgtgaccac catggccacc gcccaattta aatttggcga cgccaggagg cagatccagt
     2401 tcgtcgtggg ccaccagaat ttcgtccgga ttaatgcgga aaaaactggc catcgccgca
     2461 acggctttgc cgctgagatt cataaatgta gtcgggacta acaggcggac atcttcgcct
     2521 ccaagagtga ctcgcgaagt ataaccaaag aatttagcct cttcgcgcag cggagcgcgc
     2581 aaacgctctg ccagtaagtc aacgaaccag gcaccagcat tatgtcgcgt tgcggcgtat
     2641 tcagcaccgg ggttcgccag gccgacaatc aatttaatcg tcacgttttt tgtcctgagt
     2701 gtgtacataa ctggcgcgta gtttactggt tgcggccccg cttgacaaaa aactgcgtat
     2761 caaatgcaga taacgtaata attgcctgag tggactatta gaaagtcaag gtgttcaggc
     2821 gtttatttgt aaagttttgt tgaaataagg gttgtaattg tgatcacgcc cgcacataac
     2881 ccacagggtg ttgtctatac tttacacata aggaagaggg gtattccctg ttacaaccca
     2941 gaaagttccg gaggtgacat atgaaacgca aaaacgcttc gttactcggt aacgtgctca
     3001 tggggttggg gctggtggta atggtggtcg gcgtggggta ttcaatcctc aaccagttac
     3061 cacagtttaa tatgccccag tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg
     3121 ccattctctg gctggcgggt gcccgtgttg gcgggcatga acaggtgtgc gaccgttact
     3181 ggtgggttcg tcactatgac aaacgttgcc gccgtagcga taatcgccgt catagctaac
     3241 aaataatgca gtttgctgac cagtcagttt gcgctgactg gtcaatctcg tacttataaa
     3301 tccgccatcg ccgcgcgacg attcgggaag aacgcgaggc gtcccgggat cggttgaatg
     3361 cctgcgcgag ccatagtgcg cagtggctgg aattccacgt tgcacacgcg cagttcacat
     3421 ccttcgggca gacgcttcac aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc
     3481 ggaacggcat cccacttcag aatcacaatc cgtttgcctt caagacgtga ctccaggtcc
     3541 gtgaacaagc cttcagcagc agcaaaaaac agcgggccaa taacgcgcag taccagaaca
     3601 tcgtctggaa catctacgac taccggtgcc aggcgagtca tacgtgcgat acgacgcata
     3661 aacagcagcg atgccagcac gatccccacg ctgatggcaa taaccatatc aaacagcacg
     3721 gtcagcgaca tgcacagcag catgacaatg atgtcatctt tcggtgcatg acgcagcaag
     3781 tcgaccactt tatgcgcttc actcatgttc cacgccacca tcaacagcag ggctgccata
     3841 gcggaaagcg gcagccagga gagcagcggt gccagtacca gcagggcaag aataaccaga
     3901 atagagtgga tcaccgccga gataggggaa gttgccccgg cacggacgtt agcggcagaa
     3961 cgcgcgatgg cagctgtagc ggtaatacca ccaaagaacg gagcgataat attccccagt
     4021 ccctgtccaa ccagttcgct gttcgccttg tgtttcgtcc cggtcatacc atccagtacc
     4081 acggcgcaga gcagagattc gattgcgccg agcattgcca ttgagaatgc cgcaggcagc
     4141 agtgtgcgaa tagaatccca ggttagcgtg aattctgaat taggcagatc ccacggcagc
     4201 accagttgcg gcagcagttg cggaataccg ttaccctgag aaccatcggc caggacgtag
     4261 tggaattgcg aaccgatggt agcaacatgt ccgccgagca ggttaacaat ccccatcacc
     4321 gcgcaaccag ccagcaaggc cggaaggtga ccgggtaaac gaatgcccag acgcggccaa
     4381 aaaacaagaa tacctagcgt cacaatgcca atggcagcat cacccacatt aatggtcggc
     4441 agcgccataa ataatgcgcc gactttttgt agataatgtt ccgggacatg ggccatttgc
     4501 agaccgagaa aatctttaat ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa
     4561 cctaaggtga cggaaaccgg aatatactca atcaggcgac caaagcgtgc cagacccatc
     4621 agaatcaaaa agatccccga cagcaaggtc gcaaccagca gtcctgccag tccaaactgt
     4681 tgcgacacgg gatagagaat taccacaaat gccgcagtcg gaccggaaac gctaaagcgt
     4741 gacccacccg tcagagcaat gacaatcccc gcaacagctg cggtatataa accgtactgg
     4801 ggtgccacac cactaccaat agccaacgcc atcgccagcg ggatagcaat aatcccgacg
     4861 gttatcccgg caatcaggtc acgggtaaac cgtgcggcag tatatttttc tttccagcaa
     4921 gcgtcgatca gagcgcggaa aggcatcaca tgtgaggaaa atattttgtt cacaataatg
     4981 tttcatccgt gagcgcatca tctgtcaact aaatggcagg tgaaggaggc ataggtcata
     5041 caaatggata ttacagacaa aaaaacccgc cgcagcgggt ctttgagcgg gttcgattag
     5101 tgttcgaaca tggcagagat cgattcttcg ttgctgatac gacgaatcgc ttcggccagc
     5161 atacctgaca gggtcagagt acgcacgttc ggcagtgatt tgatttcatc gctcagcgga
     5221 atggtatcgc agacaacgac ttcatcaatt acagagttac gcaggttgtt cgccgcgttg
     5281 ccagagaaga tcgggtgagt cgcgtacgca aatacacgtt tagcaccacg ttctttcaga
     5341 gcttcagcag ctttacacag cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag
     5401 tcacgacctg caacgtcacc gatgatatgc atcacctggg aaacgttcgc acgcggacga
     5461 cgtttgtcga tgattgccat atcggtatcg ttcagcagct tagcgatagc gcgggcacgc
     5521 acaacgccgc cgatgtccgg agaaaccaca attgggttat ccagattcag ctgcagcatg
     5581 tcttccagca ggatcgggct accaaatacg ttatcaaccg gaacgtcgaa gaaaccctga
     5641 atctgttcag cgtgcagatc cactgtcagc acacggtcaa caccgacgct ggagaggaag
     5701 tctgcaacca ctttcgcagt gattggtaca cgagcggaac ggacgcgacg gtcctggcgc
     5761 gcatagccaa agtaggggat aacagcggtg atacgacctg cggaagcacg acgcagggca
     5821 tcaaccataa cgactaattc catcaggttg tcgttagtag gggcacaagt ggactggatg
     5881 atgaaaatat caccaccgcg tacattttcg ttgatttgta cactgacttc gccgtcgcta
     5941 aagcgaccta cagcggcgtc gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct
     6001 agttccgggg tggcgttacc agcaaaaagc ttcatatcag gcacgagaag aacctcaggc
     6061 atgcgtccat tggtggaaag aatctgccga aaactgtgcg ggccaggcat gatcctttcc
     6121 aggcggtgta ttaaagagcg cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac
     6181 tcagcttgcc cggcttaaag catggctctg tgcaatgggg aaagattaac acctttcgcc
     6241 acaaagccat tgagccattc cggggcttgc tctagcacct ggcgggcttc agactctgta
     6301 tcaaattcag caaagacaca ggcccctgtc ccagtcaggc gcgacggggc gtattctaac
     6361 agccaggaaa gcaccgcatc aacctcgcga aaacgttttc ttgcgataac ctcgcaatca
     6421 ttgctgaatt cacattttag caacgtttct attgaccttt ttggcgtatt gcgcgggagt
     6481 tcaggatctt taaaaatcac tggtgtcgga atacttacac cagggtgcgc caccagatac
     6541 cacttctctg gcggatccac cggcgttagt atttcaccaa cgccttcggc aaacgcggca
     6601 tgcccccgaa caaagacagg aacatctgcg cccagcgtca gccccatttc cgccagctca
     6661 tccatgctta gcccgcattg ccagagatga tttaatgcca ccaggaccgt cgcggcattg
     6721 gatgaaccac cgccgagacc gccgcccatc ggcaaacgct tgtcaatgct gatattcgca
     6781 ccgcttcccg tcggaagacg cccgctgtct gccgcagttt tcatcaacaa ccgcgctgcg
     6841 cgaacgatca ggttatcttc atgttccacg ccttcaacgg gcgttaacag acgaatatcc
     6901 ccatcgtcac gaagctcaat gctgatggtg tcgccgtaat caagaaactg aaacagcgtt
     6961 tgcagcgtgt ggtaaccatc cgcacgctga ccggtaatgt ataaaaacag attaagtttt
     7021 gccggagagg gccactgtgt ccgcattatt tcactatcca gttatccatt tttaacttga
     7081 tgcgttgacc accgtcggtg agttccatat tggctggcat cgcaggttgc gttttggtgt
     7141 cataaccacc ataaacaacc ttccagtttt tgccattctg gctgtaggta atttcgctca
     7201 ggcggtactg gtcgtccagt ttgtagtcgg ttgcatcacc cggtaaacct aaaatccatt
     7261 ggcgcaagct gttgagcgga attggcattc cggtcaattt gccaatcatc tcttcggcgt
     7321 catcggcggt ataacgctga cctttattgt cgactaactg cacgttaccc ggttgagcat
     7381 tcagctccag ttccgtgctg cccaatgggt tagtgagcag cagacggtag cgatcctggc
     7441 cggtttgctg ccagaaaaag cgggcgtaca ctttttgttg gtcagaaata taagcgaacg
     7501 cgccgcgagt ctgatactga ttaagattgc gcacgtcttg ctgatgctga cgccattgtg
     7561 gcgaatccgg gcttttgcca ggacctttgg gcgtggtaac ggaacaggca gtgagcacaa
     7621 gagcagccag cggtagcagg cggataagac gaaaatcggg ctggggcata gtgatgacaa
     7681 gtccttgaga tacgttgcag ttataaccct taatgctagc gttaccgtcc gctatcgtct
     7741 atgttcaagt tgtcttaatt gccagaatct aacggctttc ggcaattact ccaaaagggg
     7801 gcgctctctt ttattgatct tacgcatcct gtatgatgca agcagactaa ccatatcaac
     7861 gttggtatta tttcccgcag acatgaccct tttagcactc ggtatcaacc ataaaacggc
     7921 acctgtatcg ctgcgagaac gtgtatcgtt ttcgccggat aagctcgatc aggcgcttga
     7981 cagcctgctt gcgcagccga tggtgcaggg cggcgtggtg ctgtcgacgt gcaaccgcac
     8041 ggaactttat cttagcgttg aagagcagga taacctgcaa gaggcgttaa tccgctggct
     8101 ttgcgattat cacaatctta atgaagaaga tctgcgtaaa agcctctact ggcatcagga
     8161 taacgacgcg gttagccatt taatgcgtgt tgccagcggc ctggattcac tggttcttgg
     8221 ggagccgcag atcctcggtc aggttaaaaa agcgtttgcc gattcgcaaa aaggtcatat
     8281 gaaggccagc gaactggaac gcatgttcca gaaatctttc tctgtagcga aacgcgttcg
     8341 cactgaaaca gatatcggtg ccagtgctgt gtctgtcgct tttgcggctt gtacgctggc
     8401 gcggcagatc tttgaatcgc tctctacggt cacagtgttg ctggtaggcg cgggcgaaac
     8461 catcgagctg gtagcgcgtc atctgcgcga acataaagta cagaagatga ttatcgccaa
     8521 ccgcactcgc gaacgtgccc aaattctggc agatgaagtt ggcgcggaag tgattgccct
     8581 gagtgagatc gacgaacgtc tgcgcgaagc cgatatcatc atcagttcca ccgccagccc
     8641 gttaccgatt atcgggaaag gcatggtgga gcgcgcatta aaaagtcgtc gtaaccaacc
     8701 aatgctgttg gtggatattg ccgttccgcg cgatgttgag ccggaagttg gcaaactggc
     8761 gaatgcttat ctttatagtg tggacgatct gcaaagcatc atttcgcaca acctggcgca
     8821 gcgtaaagcc gcagcggttg aggcggaaac tattgtcgct caggaaacca gcgaatttat
     8881 ggcgtggctg cgagcacaaa gcgccagcga aaccattcgc gagtatcgca gccaggcaga
     8941 gcacgttcgc gatgagttaa ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc
     9001 gcaagccatt atgcaggatc tggcatggaa actgactaac cgcttgatcc atgcgccaac
     9061 gaaatcactt caacaggccg cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga
     9121 cagcctcggg ctggagtagc agtacatcat tttctttttt tacagggtgc atttacgcct
     9181 atgaagcctt ctatcgttgc caaactggaa gccctgcatg aacgccatga agaagttcag
     9241 gcgttgctgg gtgacgcgca aactatcgcc gaccaggaac gttttcgcgc attatcacgc
     9301 gaatatgcgc agttaagtga tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa
     9361 gatatcgaaa ccgcacagat gatgctcgat gatcctgaaa tgcgtgagat ggcgcaggat
     9421 gaactgcgcg aagctaaaga aaaaagcgag caactggaac agcaattaca ggttctgtta
     9481 ctgccaaaag atcctgatga cgaacgtaac gccttcctcg aagtccgagc cggaaccggc
     9541 ggcgacgaag cggcgctgtt cgcgggcgat ctgttccgta tgtacagccg ttatgccgaa
     9601 gcccgccgct ggcgggtaga aatcatgagc gccagcgagg gtgaacatgg tggttataaa
     9661 gagatcatcg ccaaaattag cggtgatggt gtgtatggtc gtctgaaatt tgaatccggc
     9721 ggtcatcgcg tgcagcgtgt tcctgctacg gaatcgcagg gtcgtattca tacttctgct
     9781 tgtaccgttg cggtaatgcc agaactgcct gacgcagaac tgccggacat caacccagca
     9841 gatttacgca ttgatacttt ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc
     9901 gattcggcaa ttcgtattac tcacttgccg accgggattg ttgttgaatg tcaggacgaa
     9961 cgttcacaac ataaaaacaa agctaaagca ctttctgtac ttggtgctcg tatccacgct
    10021 gcagaaatgg caaaacggca acaggccgaa gcgtctaccc gtcgtaacct gctggggagt
    10081 ggcgatcgca gcgaccgtaa ccgtacttac aacttcccgc aggggcgcgt taccgatcac
    10141 cgcatcaacc tgacgctcta ccgtctggat gaagtgatgg aaggtaagct ggatatgctg
    10201 attgaaccga ttatccagga acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa
    10261 taatggaata tcaatactgg ttacgtgaag caataagcca acttcaggcg agcgaaagcc
    10321 cacgccgtga cgctgaaatc ttattggagt atgtcactgg cagggggcgg acttttattc
    10381 tggcctttgg tgaaacgcag ctgactgacg aacaatgtca gcaacttgat gcgctactga
    10441 cacgtcgtcg cgatggtgaa cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt
    10501 tgccgttatt tgtttcgcca gcgaccttaa ttccgcgccc ggatacggag tgtctggtgg
    10561 agcaggcact ggcgcggttg cctgaacaac cttgccgtat tctcgatctc gggacgggta
    10621 ccggggcgat tgcgcttgcg ctggctagcg agcgcccgga ctgcgaaatt atcgctgtag
    10681 atcgtatgcc tgatgctgtc tccctggcac aacgtaatgc ccagcatctg gcgatcaaaa
    10741 atatccacat tctgcaaagc gactggttta gcgcgctagc cgggcagcag tttgcgatga
    10801 ttgtcagcaa tccgccgtat attgacgagc aggacccaca tcttcaacaa ggcgatgtcc
    10861 gctttgagcc gctcactgcg ctggttgcgg cagacagtgg aatggcagac atcgtgcata
    10921 tcatcgaaca gtcgcgtaac gcgctggtat ccggcggctt tctgcttctg gaacatggct
    10981 ggcagcaggg cgaagcggtg cgacaagcat ttatcctcgc ggggtatcat gacgtcgaaa
    11041 cctgccgtga ctatggtgat aacgagcgcg taacgctcgg ccgctattat caatgacaag
    11101 tttttctaca ctgcttagtg ttcatcttat tagtatcgcg ctttctgttg ggctattaac
    11161 cttacgtttc tggctacgtt atcagaagca tccacaggca tttgctcgct ggacgcgcat
    11221 tgtgccgccg gttgtcgata cggtgttatt gttaagcggc attgcgttga tggctaaagc
    11281 gcacatcctg ccattttccg ggcaggcaca gtggctgact gaaaagctgt ttggagttat
    11341 catttatatc gttttgggtt ttattgcact cgattatcgt cgtatgcaca gtcagcaggc
    11401 gcgcattatt gccttcccgc tggcgttggt ggtgctgtac atcatcatta aactcgccac
    11461 cacaaaagta ccgttactgg ggtaagtcat gagatcgtta gctgatttcg aatttaataa
    11521 agcgccattg tgcgaaggca tgatcctggc ttgcgaagca atccgccgcg attttccctc
    11581 gcaagatgtt tacgacgaac tggagcgtct cgttagtctg gcgaaggaag aaatcagcca
    11641 gcttctgcct ttagaagagc agctggaaaa actgatcgcg ctgttttacg gcgactgggg
    11701 atttaaagcc tcacgcggcg tttatcgtct ttccgatgca ttatggctgg accaggtgtt
    11761 aaagaatcga caggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt
    11821 ctcgatttgc cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt
    11881 ccggatggcg aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg
    11941 ctggacgtat ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt
    12001 gatgaagctg ataacattga ggtaatccgc aaattgttgg atacactcaa agcctcgttg
    12061 atggaagaaa atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac
    12121 ccggaagatc cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa
    12181 cacgttgcgt tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc
    12241 gaaatgatcc gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta
    12301 atcgacattt tactcaagat taaggcgatc ctatgaaaca aaaagtggtt agcattggcg
    12361 acatcaacgt agcaaatgac ctgccgttcg tactgtttgg tggtatgaac gtgctggaat
    12421 ctcgcgatct ggcgatgcgc atttgcgagc actacgtaac cgtgacccag aaactgggta
    12481 tcccttacgt gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc
    12541 gtggaccggg cctggaagaa gggatgaaaa tcttccagga gttgaagcag acttttggcg
    12601 tgaaaattat caccgacgtt cacgaaccaa gtcaggcaca gcccgttgct gatgtcgtgg
    12661 atgtgattca gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga
    12721 aaaccggtgc ggtaattaac gtcaagaaac cacagtttgt cagcccggga cagatgggta
    12781 atatcgttga taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgcggtg
    12841 ctaacttcgg ctatgacaac ctggttgtcg atatgctggg cttcagcatt atgaagaaag
    12901 tgtctggtaa ctcgccggtg attttcgacg tgacccacgc actgcaatgc cgcgatccgt
    12961 ttggcgcagc ttccggtggt cgtcgtgctc aggtggctga gctggcacga gccggtatgg
    13021 cggtaggtct ggcggggctg tttattgaag cgcatccgga tccggaacat gcgaaatgtg
    13081 atggtccatc cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag atgaaagcga
    13141 ttgatgatct ggtgaaaggt ttcgaagaac tggataccag caagtaatct tttttgcttg
    13201 aaaaataaag ccttagcgtt ctgcgttaag acttttttca tgggtgccag atacaaaaa
//
LOCUS       DAJTXQ010000112        12999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000112 DAJTXQ010000000
VERSION     DAJTXQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.112"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..192
                     /locus_tag="OO515_003140"
     CDS             <1..192
                     /locus_tag="OO515_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577001.1"
                     /translation="RFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLR
                     QAGCPTVYPLALASTSVKN"
     gene            99..1394
                     /locus_tag="OO515_003141"
     CDS             99..1394
                     /locus_tag="OO515_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6577002.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1440..2033)
                     /gene="yhgN"
                     /locus_tag="OO515_003142"
     CDS             complement(1440..2033)
                     /gene="yhgN"
                     /locus_tag="OO515_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6577003.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2225..3328
                     /gene="asd"
                     /locus_tag="OO515_003143"
     CDS             2225..3328
                     /gene="asd"
                     /locus_tag="OO515_003143"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6577004.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3601..5787
                     /gene="glgB"
                     /locus_tag="OO515_003144"
     CDS             3601..5787
                     /gene="glgB"
                     /locus_tag="OO515_003144"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6577005.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5784..7757
                     /gene="glgX"
                     /locus_tag="OO515_003145"
     CDS             5784..7757
                     /gene="glgX"
                     /locus_tag="OO515_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6577006.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7775..9070
                     /gene="glgC"
                     /locus_tag="OO515_003146"
     CDS             7775..9070
                     /gene="glgC"
                     /locus_tag="OO515_003146"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6577007.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9070..10503
                     /gene="glgA"
                     /locus_tag="OO515_003147"
     CDS             9070..10503
                     /gene="glgA"
                     /locus_tag="OO515_003147"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6577008.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10522..12969
                     /gene="glgP"
                     /locus_tag="OO515_003148"
     CDS             10522..12969
                     /gene="glgP"
                     /locus_tag="OO515_003148"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6577009.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3229 c         3577 g         3153 t
ORIGIN      
        1 cgtttccacc agtgtcaaaa tctcgacggg gcgtttgtta ttacccctcc tttgatgcct
       61 ggtccggcgt tgctggttga cgatatcgtg gattctgcat ggacgctgac agttctgaca
      121 gcactgttac gccaggcagg ttgcccgacg gtttatcctc ttgcccttgc ttctacctcg
      181 gtaaaaaatt gatgaatctt tcagccaatg cacaagcaac tctcctgcta accagcgatt
      241 tttctcgcgc ggcggcgagt gagtataaac ctcttagtaa tagtgaatgg gggaagtttg
      301 cattatggct gaagcaccaa cgtatcagtc ccgccgagct tctggtgccg caaccgcaag
      361 agaaacttac aggctggagc gatccgcgta tttctcagga gcgtattctt ggcttgctgg
      421 cgcgtggtca tagtctggcg ttggcggtag ataagtggca acgcgccggt ttatggatct
      481 taacccgcgg agatgctgat tatcccgttc gcttgaaaaa ccgattgcga acggatgcac
      541 ctcccgtttt atttggctgc gggaataaag cattactgca agcggaaggt atggcgattg
      601 ttggctcgcg agatgctccg actgacgatt tgcgctatac ccaacaactg gccgcgaaac
      661 tggcccaaca ggggatttgc gttatctctg gtggtgcgcg aggtattgat gaatgtgcaa
      721 tggcgtcggc actggaggcc gggggaactg ccgttggcgt attagctgat agcttgttaa
      781 aaacgagtac gttagtgaaa tggcgtgaag ggcttatagc aggcaacctg gtgttgattt
      841 cgccgtttta cccagaggta cgtttcaccg tcggcaatgc gatggcgcga aataaatata
      901 tttattgcct tgctgaaagc gcaatggttg tacgtgcggg aatgaccggt ggaacgataa
      961 ccggggcgat ggaggcatta aaacatcagt ggctgcctgt gcaggttaaa ccaaatcagg
     1021 atatgcaatc agccaattca cgattagtag aaaatggggc gtcatggagt gctgaacagg
     1081 ctgagaatgt gacgatcaga ctgccagacg ttcctgggct gatgtatgac agagcactcc
     1141 gtaacgctca accagaactg ttttcgctgc atgaagatga cgcaaattac gcagtaatgc
     1201 ccgcgtatac gcctgtcgat ttttatcaac tctttgtggc ggaactggcg atccttgcaa
     1261 aggaatcgat aagtattgaa aggctggcgt cttgtactgg tttaaccatc gaacaaatta
     1321 gtgtgtggct gaaccgcgca gaagaagagg gaagggttat ccgattgggc gaaggtcatt
     1381 atcagttcag gtaataaaac aggtgccgcg tctggttgtg tgcagcacct gtttgaacct
     1441 taccccttca tccacattcg aatgccgtcg aggaacatct gggttgccat catcaccaga
     1501 atcaatccca tcaggcgttc aagtgcgttc acccctttct cgcccagcag acgtaaaaat
     1561 agcgaagact gtagcaggat gacaaaggtg ccgccccagg ccagcagcag agcaatcacc
     1621 agatgtccca tctgattcgg gtactgatga gacaacaaca tcagcgtggc gagaatagtc
     1681 ggcccggcga ctaacggaat tgccaacggc acgataaatg gctcttcacc tgccggaagc
     1741 ccgctgctat ttcctgaagc gctggggaaa atcatcttaa tggcgattag aaacagaatg
     1801 atgccgccag aaatggagac ggtttctgct cgcaggctga gaaatgccag aattttctca
     1861 cccgcaaaca ggaacaccag catcaccagg agagcaataa gcaactctcg caccatgatt
     1921 gcccgccgtc ttttcggttc agtatgtttc agtacggaca tgaaaatagg caggtttccg
     1981 agcggatcca taatcaggat caataaaact gctgcagaaa tgattgcatt cataactcaa
     2041 attccctgat aattgccgcg gactttctgc gtgctaacaa agcaggataa gtcgcattac
     2101 tgatggcttc gctatcattg attaatttca cttgcgactt tggctgcttt ttgtatggtg
     2161 aaagatgtgc caagaggaga ccggcacatt tatacagcac acatctttgc aggaaaaaac
     2221 gcttatgaaa aatgttggtt ttatcggctg gcgcggtatg gtcggctccg ttctcatgca
     2281 acgcatggtt gaagagcgcg acttcgacgc cattcgccct gtcttctttt ctacttctca
     2341 gcttggccag gctgcgccgt cttttggcgg aaccactggc acacttcagg atgcctttga
     2401 tctggaggcg ctaaaggccc tcgatatcat tgtgacctgt cagggcggcg attataccaa
     2461 cgaaatctat ccaaagcttc gtgaaagcgg atggcaaggt tactggattg acgcagcatc
     2521 gtctctgcgc atgaaagatg acgccatcat cattcttgac cccgtcaatc aggacgtcat
     2581 taccgacgga ttaaataatg gcatcaggac ttttgttggc ggtaactgta ccgtaagcct
     2641 gatgttgatg tcgttgggtg gtttattcgc caatgatctt gttgattggg tgtccgttgc
     2701 aacctaccag gccgcttccg gcggtggtgc gcgacatatg cgtgagttat taacccagat
     2761 gggccatctg tatggccatg tggcagatga actcgcgacc ccgtcctctg ctattctcga
     2821 tatcgaacgc aaagtcacaa ccttaacccg tagcggtgag ctgccggtgg ataactttgg
     2881 cgtgccgctg gcgggtagcc tgattccgtg gatcgacaaa cagctcgata acggtcagag
     2941 ccgcgaagag tggaaagggc aggcggaaac caacaagatc ctcaacacat cttccgtaat
     3001 tccggtagat ggtttatgtg tgcgtgtcgg ggcattgcgc tgccacagcc aggcattcac
     3061 tattaaattg aaaaaagatg tgtctattcc gaccgtcgaa gaactgctgg ctgcgcacaa
     3121 tccgtgggcg aaagtcgttc cgaacgatcg ggaaatcact atgcgtgagc taaccccagc
     3181 tgccgttacc ggcacgctga ccacgccggt aggccgcctg cgtaagctga atatgggacc
     3241 agagttcctg tcagccttta ccgtgggcga ccagctgctg tggggggccg cggagccgct
     3301 gcgtcggatg cttcgtcaac tggcgtaatc tttattcatt aaatctgggg cgcgatgccg
     3361 cccctgttag tgcgtaatac aggagtaagc gcagatgttt catgatttac cgggagttaa
     3421 atagagcatt ggctattctt taagggtggc tgaatacatg agtattcaca gccttacctg
     3481 aagtgaggac gacgcagaga ggatgcacag agtgctgcgc cgttcaggtc aaaaaaatgt
     3541 cacaaccaga agtcaaaaat ccaattggat ggggtgacac aataaaacag gaagacaagc
     3601 atgtccgatc gtatcgatag agacgtgatt aacgcgctaa ttgcaggcca ttttgcggat
     3661 cctttttccg tactgggaat gcataaaacc accgcgggac tggaagtccg tgccctttta
     3721 cccgacgcta ccgatgtgtg ggtgattgaa ccgaaaaccg ggcgcaaact cgcaaaactg
     3781 gagtgtctcg actcacgggg attctttagc ggcgtcattc cgcgacgtaa gaattttttc
     3841 cgctatcagt tggctgttgt ctggcatggt cagcaaaacc tgattgatga tccttaccgt
     3901 tttggtccgc taatccagga aatggatgcc tggctattat ctgaaggtac tcacctgcgc
     3961 ccgtatgaaa ccttaggcgc gcatgcagat actatggatg gcgtcacagg tacgcgtttt
     4021 tctgtctggg ctccaaacgc ccgtcgggtc tcggtggttg ggcaattcaa ctactgggac
     4081 ggtcgccgtc acccgatgcg cctgcgtaaa gagagcggca tttgggaact gtttatccct
     4141 ggggcgcata acggtcagct ctataaatac gagatgattg atgccaatgg caacttgcgt
     4201 ctgaagtccg acccttatgc cttcgaagcg caaatgcgcc cggaaaccgc gtctcttatt
     4261 tgcgggctgc cggaaaaggt tgtacagact gaagagcgca aaaaagcgaa tcagtttgat
     4321 gcgccaatct ctatttatga agttcacctg ggctcctggc gtcgccacac cgataacaat
     4381 ttctggttga gctaccgcga gctggccgat caactggtgc cttatgctaa atggatgggc
     4441 tttacccacc tcgaactact gcccattaac gagcatccct tcgatggcag ttggggttat
     4501 cagccaaccg gcctgtatgc accaacccgc cgttttggta ctcgcgacga cttccgttat
     4561 ttcattgatg ccgcacacgc agcgggtctg aacgtgattc tcgactgggt gccaggccac
     4621 ttcccgaccg atgactttgc gcttgccgaa tttgatggca cgaacttgta tgaacacagc
     4681 gatccgcgcg aaggctatca tcaggactgg aatacgctga tctacaacta tggtcgccgt
     4741 gaagtcagta acttcctcgt cggtaacgcg ctttactgga ttgaacgttt tggtattgat
     4801 gcgctgcgcg tcgatgcggt ggcgtcaatg atttatcgcg actacagccg taaagagggg
     4861 gagtggatcc cgaacgaatt tggcgggcgc gagaatcttg aagcgattga attcttgcgt
     4921 aataccaacc gtattcttgg tgagcaggtt tccggtgcgg tgacaatggc tgaggagtct
     4981 accgatttcc ctggcgtttc tcgtccgcag gatatgggcg gtctgggctt ctggtacaag
     5041 tggaacctcg gctggatgca tgacaccctg gactacatga agctcgaccc ggtttatcgt
     5101 cagtatcatc acgataaact caccttcggg attctctaca actacactga aaacttcgtc
     5161 ctgccgttgt cgcatgatga agtggtccac ggtaaaaaat cgattctcga ccgcatgccg
     5221 ggcgacgcat ggcagaaatt cgctaacctg cgcgcctact atggctggat gtgggcattc
     5281 ccgggcaaga aactgctgtt catggggaac gaatttgccc aaggccgtga gtggaaccat
     5341 gacgccagcc tcgactggca tctgttggaa ggcggcgata actggcacca cggtgtccag
     5401 cgtctggtgc gcgatctgaa cctcacctac cgccaccata aagcaatgca tgaactggat
     5461 tttgacccgt acggctttga atggctagtg gtggatgaca aagaacgctc ggtgctgatc
     5521 tttgtgcgtc gcgataaaga gggtaacgaa attatcgttg ccagtaactt tacgccggtg
     5581 ccgcgtcatg attatcgctt cggcattaat cagccaggca aatggcgtga aatcctcaat
     5641 accgattcca tgcactatca cggcagtaat gcaggcaatg gcggcacggt acacagcgat
     5701 gagattgcca gccacggtcg tcagcattca ctaagcctga cactaccacc gctggccact
     5761 atctggctgg ttcgggaggc agaatgacac gactcgccat tggcaaaccc gctcccctcg
     5821 gcgcgcatta cgacggtcag ggcgtcaact tcacactttt ctccgctcat gccgagcggg
     5881 tagagctgtg tgtctttgac gccaatggcc aggaacatcg ctatgacttg ccagggcaca
     5941 gtggcgacat ttggcacggt tatctgccgg atgcgcgccc gggtttgcgt tatggttatc
     6001 gcgttcatgg cccctggcaa cccgccgagg ggcatcgctt taacccggcg aagttgttga
     6061 ttgatccttg cgcgcggcaa attgaagggg aattcaaaga taacccgctg ctgcacgccg
     6121 gtcataatga acctgactat cgcgacaacg ccgccattgc gccgaaatgc gtagtggtgg
     6181 ttgatcacta tgactgggaa gatgatgccc cgccgcgcac gccgtggggc agcaccatca
     6241 tttatgaagc ccatgttaaa ggattaacgt acctgcaccc ggagatcccg gtcgagatcc
     6301 gtggcactta taaagccctc gggcatccgg tgatgatcaa ctatttgaaa cagttgggca
     6361 ttaccgcgct ggaactgctg ccggtggcgc agtttgccag tgaaccacgt ctgcaacgca
     6421 tggggctaag taactactgg ggttacaacc cggtggcgat gtttgcgctg catccggcgt
     6481 atgcctgctc gccagaaacg gcgctgcatg agtttcgcga tgcaatcaaa gcactgcata
     6541 aagcgggtat cgaagtcatt cttgatatcg tgctcaacca tagtgcggaa ctggacctcg
     6601 acggcccgtt attctcgctg cgtgggatcg acaaccgtag ctattattgg ataagagaag
     6661 acggcgatta tcacaactgg accggttgtg gcaacacgct caatttgagt catccggcgg
     6721 tggtggatta tgcctgcgcc tgcctgcgtt attgggtaga aacctgtcac gtcgatggtt
     6781 tccgctttga tctggcggca gtcatgggcc gtacgccaga gttccgtcag gatgcgccgt
     6841 tgtttaccgc tatccagaac tgcccggtgc tctcgcaggt gaagttaatt gctgaaccgt
     6901 gggatatcgc ccttggtggt tatcaggtgg gaaatttccc gccgctgttt gccgagtgga
     6961 acgatcattt ccgcgatgct gcccgtcgtt tctggctaca ttatgatttg cctctggggg
     7021 cgtttgccgg gcgttttgct gcctccagcg atgtttttaa acgtaatggt cgtctgccga
     7081 gtgccgcgat taatctcgtc actgcgcatg acggttttac gcttcgcgac tgcgtttgct
     7141 tcaaccataa acacaatgaa gcaaacggag aagaaaatcg cgacgggacc aacaacaatt
     7201 acagtaacaa tcatggtaaa gaagggttag gcggttctct tgacctggtt gaacggcggc
     7261 gcgacagtat tcacgccctg ttaacaacgt tgttgctctc ccagggtacg ccgatgttac
     7321 tggccggtga cgaacacggt cacagccagc atggcaataa caatgcctac tgtcaggata
     7381 accaattaac ctggttggac tggtcgcagg caagcagtgg tttaaccgca tttaccgccg
     7441 cgttaatcca tctgcgcaag cgcattcccg ctttggtgga gaatcgctgg tgggaagaag
     7501 gcgacggcaa tgtccgttgg ctaaatcgat atgctcaacc tttaagcacg gatgagtggc
     7561 aaaacgggcc gaaacagctg caaattctgc tctcggatcg ctttttgatc gcaattaacg
     7621 ccacgcttga ggtaacagag attgttttac ctgctgggga gtggcacgcc attcccccat
     7681 tcgctggaga ggataaccca gtgattacgg ctgtctggca gggacctgca cacggattgt
     7741 gtgtgttcca gagatgataa aaaaggagtt agtcatggtt agtttagaga agaacgatca
     7801 cttaatgttg gcgcgccagc tgccattgaa atctgttgcc ctgatactgg cgggaggacg
     7861 tggtacccgc ctgaaggatt taaccaataa gcgagcaaaa ccggccgtac acttcggcgg
     7921 taagttccgc attattgact ttgcgctgtc taactgcatc aactctggga tccgtcgtat
     7981 gggcgtgatc acccagtacc agtcccacac tctggtgcag cacattcagc gcggctggtc
     8041 attcttcaat gaagaaatga acgagtttgt cgatctgctg ccagcacagc agagaatgaa
     8101 aggggaaaac tggtatcgcg gcaccgcaga tgcggtcacc caaaacctcg acattatccg
     8161 ccgttataaa gcggaatacg tggtgatcct ggcgggcgac catatctaca agcaagacta
     8221 ctcgcgtatg cttatcgatc acgtcgaaaa aggcgcacgt tgcaccgttg cttgtatgcc
     8281 agtaccgatt gaagaagcct ccgcatttgg cgttatggcg gttgatgaga acgataaaat
     8341 tatcgaattc gttgaaaaac ctgctaaccc accgtcaatg ccgaacgatc cgagcaaatc
     8401 tctggcgagt atgggtatct acgtctttga cgccgactat ctgtatgaac tgctggaaga
     8461 agacgatcgt gatgagaact ccagccacga ctttggcaaa gatttgattc ccaagatcac
     8521 cgaagccggt ctggcctatg cgcacccgtt cccgctctct tgcgtacaat ccgacccgga
     8581 tgccgagccg tactggcgcg atgtgggtac gctggaagct tactggaaag cgaacctcga
     8641 tctggcctct gtggtgccgg aactggatat gtacgatcgc aattggccaa ttcgcaccta
     8701 caatgaatca ttaccgccag cgaaattcgt gcaggatcgc tccggtagcc acgggatgac
     8761 ccttaactca ctggtatccg gcggctgtgt gatctccggt tcggtggtgg tgcagtccgt
     8821 tctgttctcg cgcgttcgcg tgaattcatt ctgcaacatt gattccgccg tattgttacc
     8881 ggaagtatgg gtaggtcgct cgtgccgtct gcgccgctgc gtcatcgatc gtgcttgtgt
     8941 tattccggaa ggcatggtga ttggtgaaaa cgcagaggaa gatgcacgtc gtttctatcg
     9001 ttcagaagaa ggcatcgtgc tggtaacgcg cgaaatgcta cggaagttag ggcataaaca
     9061 ggagcgataa tgcaggtttt acatgtatgt tcagagatgt tcccgctgct taaaaccggc
     9121 ggtctggctg atgttattgg ggcattaccc gcagcacaaa tcgcagacgg cgttgacgct
     9181 cgcgtactgt tgcctgcatt tcccgatatt cgccgtggcg tgaccgatgc gcaggtagta
     9241 tcccgtcgtg ataccttcgc cggacatatc acgctgttgt tcggtcatta caacggggtt
     9301 ggcatatacc tgattgacgc gccgcatctc tatgatcgtc cgggaagccc gtatcacgat
     9361 accaacttat ttgcctatac cgacaacgta ttgcgttttg cgcttctggg gtgggttggg
     9421 gcagaaatgg ccagcgggct tgacccattc tggcgtcctg atgtggtgca tgcgcacgac
     9481 tggcatgcag gccttgcgcc tgcgtatctg gcggcgcgcg ggcgtcctgc gaagtcggtg
     9541 tttactgtgc acaacctggc ctatcaaggc atgttttatg cacatcacat gaatgacatc
     9601 caattgccat ggtcattctt taatattcat gggctggaat tcaacggaca aatctctttc
     9661 ctgaaggccg gtctgtacta tgccgatcac attacggcgg tcagtccaac ctacgctcgc
     9721 gagatcaccg aaccgcagtt tgcctacggt atggaaggtc tgttgcaaca gcgtcatcgt
     9781 gaagggcgtc tttccggcgt actgaacggc gtggacgaga aaatctggag tccagagacg
     9841 gacttactgt tggcctcgcg ctacacccgc gatacgttgg aagataaagc ggaaaataag
     9901 cgccagttac aaatcgcaat ggggcttaag gttgacgata aagtgccgct ttttgcagtg
     9961 gtgagccgtc tgaccagcca gaaaggtctc gacctggtgc tggaagcctt accgggtctt
    10021 ctggagcagg gcgggcagct ggcgctactc ggcgcgggcg atccggtgct gcaggaaggt
    10081 ttccttgcgg cggcagcgga ataccccggt caggtgggcg ttcagattgg ctatcacgaa
    10141 gcattttcgc atcgcattat gggcggcgcg gacgtcattc tggtgcccag ccgttttgaa
    10201 ccgtgcggct taacgcaact ttatggattg aagtacggta cgctgccgtt agtgcggcgc
    10261 accggtgggc ttgctgatac ggtttctgac tgttctcttg agaaccttgc agatggcgtc
    10321 gccagtgggt ttgtctttga agataataat gcctggtcgc tgttacgggc tattcggcgt
    10381 gcttttgtac tgtggtcccg tccttcactg tggcggtttg tgcaacgtca ggctatggca
    10441 atggatttta gctggcaggt cgcggcgaag tcgtaccgtg agctttacta tcgcttgaaa
    10501 tagttttcag gaaacgccta tatgaatgct ccgtttacat attcatcgcc cacgcttagc
    10561 gtagaagctc ttaagcactc tatcgcttac aagctgatgt ttacgattgg aaaggacccg
    10621 gtcgtcgcca ataaacatga atggctgaac gcaacgttat ttgctgtgcg cgatcgtctc
    10681 gtggagcgct ggttacgttc aaaccgtgcc cagttgtcgc aagaaacacg tcaggtttac
    10741 tacctgtcga tggagttttt gattggccgt acgctctcca acgccatgtt gtcgctagga
    10801 atttacgaag atgtacaggg cgcactggaa gcgatggggt taaatctcga agagctgatt
    10861 gatgaagaaa atgacccagg cctcggtaac ggtggcctgg gacgtctggc ggcttgcttc
    10921 cttgattctc tggcgacgtt agggttgccg gggcgcggtt acggcatccg ctatgactac
    10981 ggtatgttca agcagaacat cgttaacggt agccagaaag agtcgccaga ctactggctg
    11041 gaatacggta acccgtggga attcaaacgc cacaacacgc gctataaagt ccgttttggc
    11101 ggtcgcattc agcaggaagg taaaaaaacg cgctggattg aaaccgaaga gattctggga
    11161 gtcgcttacg atcagataat ccctggttac gacaccgacg cgaccaacac gctgcgtttg
    11221 tggagtgcgc aagccagtag cgaaattaac ctcggtaaat tcaaccaggg tgactacttc
    11281 gcggcagtgg aagataaaaa ccactccgag aacgtatctc gcgtactgta tccggatgac
    11341 tccacctact ccgggcgtga gctgcgcctg cgtcaggaat acttcctggt ttcctcgacc
    11401 attcaggaca ttttaagccg ccattatcag ttgcataaaa cctacgataa cctggcggat
    11461 aaaatcgcga ttcatctcaa tgatacccat ccggtactgt cgattcctga gatgatgcgt
    11521 ctgctgatcg atgagcacca atttagctgg gacgacgcgt ttgaggtgtg ttgtcaggtc
    11581 ttctcctaca ctaaccacac gctgatgagc gaggcgctgg aaacctggcc ggttgatatg
    11641 ctgggtaaaa ttctgccgcg tcacctgcag atcatctttg aaatcaacga ctatttcctg
    11701 aaaaccttgc aggaacagta tccgaacgat accgatctgc tgggacgggc gtcgatcatt
    11761 gatgaatcca acggtcgtcg tgtgcgtatg gcctggctgg cggttgttgt gagccacaaa
    11821 gttaacggtg tatcggaact gcactctaat ctgatggtgc aatcgttgtt tgccgacttt
    11881 gcgaaaatct tcccgggtcg tttcaccaac gtcaccaacg gtgtgacgcc gcgtcgctgg
    11941 ctggcggtag cgaacccatc gctttcagcc gtgctggacg aacacctggg ccgtaactgg
    12001 cgcaccgacc ttagcctgct taatgagctg caacaacact gtgatttccc aatggttaat
    12061 cacgctgtgc atcaggcgaa gctggagaac aaaaagcgtc tggcagagta tatcgcccag
    12121 cagctgaatg tggtggtgaa tccaaaggcg ttgttcgatg tacaaatcaa acgtattcac
    12181 gaatacaaac gtcaattgat gaatgtgttg catgtgatta cccgctataa ccgcatcaag
    12241 gccgacccgg atgcgaagtg ggtaccgcgc gtgaatattt ttggcggtaa ggcggcttcg
    12301 gcctattaca tggcgaagca cattattcat ttgatcaatg acgtagcgaa agtgatcaac
    12361 aacgatccgc agattggcga taagctgaaa gtcgtgttca tcccgaacta cagcgttagc
    12421 ctggcgcagt tgatcattcc ggcggcagat ctgtctgaac agatttcgct ggcagggacg
    12481 gaagcttccg gcaccagtaa catgaagttt gcgcttaacg gtgcgctgac tatcggtacg
    12541 ttggacggtg cgaatgtcga gatgcgggat catgtcggtg ctgacaatat ctttattttt
    12601 ggtaacacag cggaagaagt ggaagaactg cgtcgtcagg gctacaaacc gcgtgaatac
    12661 tacgagaaag atgaggagct gcatcaggtg ctgacgcaaa tcggcagcgg tgtattcagt
    12721 ccggaagatc cgggtcgcta tcgcgatctg gttgattcgc tgatcaactt cggcgatcac
    12781 tatcaggttc tggcggatta tcgcagctat gtcgattgtc aggataaagt cgacgaactc
    12841 tacgagcttc aggaagagtg gaccgcaaaa gcgatgctga acattgccaa tatgggctac
    12901 ttctcttctg accgtactat caaagagtac gccgatcata tctggcatat cgatccggtg
    12961 agattgtaag ttcaccaata aatagaacgg ggccaaagg
//
LOCUS       DAJTXQ010000113        12447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000113 DAJTXQ010000000
VERSION     DAJTXQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.113"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="OO515_003149"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="OO515_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6577010.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="OO515_003150"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="OO515_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6577011.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="OO515_003151"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="OO515_003151"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6577012.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="OO515_003152"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="OO515_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="OO515_003153"
     CDS             3566..3850
                     /locus_tag="OO515_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6577013.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO515_003154"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO515_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6577014.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO515_003155"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO515_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6577015.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OO515_003156"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OO515_003156"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6577016.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="OO515_003157"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="OO515_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6577017.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10040..11140
                     /gene="ompC"
                     /locus_tag="OO515_003158"
     CDS             10040..11140
                     /gene="ompC"
                     /locus_tag="OO515_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6577018.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11304..11786
                     /locus_tag="OO515_003159"
                     /pseudo
     CDS             11304..11786
                     /locus_tag="OO515_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11901..>12447
                     /locus_tag="OO515_003160"
     CDS             11901..>12447
                     /locus_tag="OO515_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6577019.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT         3229 a         3104 c         2968 g         3146 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca atattaaaaa
     9841 ataaatttca aataccagta attcacctta tatctcattt cgacttcgat cacatatcga
     9901 taaataatat tatttcatct atttttctca ttgtttttaa tcaccattct attttaaaaa
     9961 cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata
    10021 attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt
    10081 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct
    10141 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
    10201 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
    10261 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa
    10321 caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg
    10381 tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg
    10441 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac
    10501 ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca
    10561 aggcaaaaat gaacgtactg acaacagtca tctttatggt gctgactaca cgcgtgccaa
    10621 cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
    10681 atataccaaa tccgatcgga caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc
    10741 ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta
    10801 ctttgcagct aattacgctg aaacattaaa catgaccacc tatggggatg gttatatctc
    10861 taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc
    10921 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacggcg accaggacat
    10981 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
    11041 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcggac
    11101 cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc
    11161 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
    11221 atttttgata tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat
    11281 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
    11341 ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt
    11401 tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa
    11461 ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
    11521 atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa
    11581 acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca
    11641 gcgttaataa aattaacacg ataacctttc caaaagaatc aaaactggaa aacatttata
    11701 ttgatcataa caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg
    11761 aagcgcaaaa taacaaactg acaaaaatta atatttctga tagttataaa ctgaaatttc
    11821 ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa tctctgattg
    11881 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
    11941 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
    12001 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
    12061 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
    12121 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
    12181 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
    12241 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
    12301 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
    12361 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
    12421 cagttactta ttttagtgcg gcacata
//
LOCUS       DAJTXQ010000114        12409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000114 DAJTXQ010000000
VERSION     DAJTXQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.114"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO515_003161"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO515_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6577020.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO515_003162"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO515_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6577021.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OO515_003163"
     CDS             complement(2659..5772)
                     /locus_tag="OO515_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6577022.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO515_003164"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO515_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6577023.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OO515_003165"
     CDS             7014..7292
                     /locus_tag="OO515_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577024.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO515_003166"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO515_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6577025.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO515_003167"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO515_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6577026.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OO515_003168"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OO515_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6577027.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OO515_003169"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OO515_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6577028.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OO515_003170"
     CDS             10284..10931
                     /locus_tag="OO515_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR6577029.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="OO515_003171"
     CDS             complement(10973..11503)
                     /locus_tag="OO515_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6577030.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO515_003172"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO515_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6577031.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2753 c         2808 g         3418 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAJTXQ010000115        12349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000115 DAJTXQ010000000
VERSION     DAJTXQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.115"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(111..320)
                     /locus_tag="OO515_003173"
                     /pseudo
     CDS             complement(111..320)
                     /locus_tag="OO515_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(522..1166)
                     /gene="cheZ"
                     /locus_tag="OO515_003174"
     CDS             complement(522..1166)
                     /gene="cheZ"
                     /locus_tag="OO515_003174"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6577032.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1177..1566)
                     /gene="cheY"
                     /locus_tag="OO515_003175"
     CDS             complement(1177..1566)
                     /gene="cheY"
                     /locus_tag="OO515_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6577033.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1581..2630)
                     /gene="cheB"
                     /locus_tag="OO515_003176"
     CDS             complement(1581..2630)
                     /gene="cheB"
                     /locus_tag="OO515_003176"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6577034.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2633..3493)
                     /gene="cheR"
                     /locus_tag="OO515_003177"
     CDS             complement(2633..3493)
                     /gene="cheR"
                     /locus_tag="OO515_003177"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6577035.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3512..5113)
                     /gene="tap"
                     /locus_tag="OO515_003178"
     CDS             complement(3512..5113)
                     /gene="tap"
                     /locus_tag="OO515_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6577036.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5159..6820)
                     /gene="tar"
                     /locus_tag="OO515_003179"
     CDS             complement(5159..6820)
                     /gene="tar"
                     /locus_tag="OO515_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6577037.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6965..7468)
                     /gene="cheW"
                     /locus_tag="OO515_003180"
     CDS             complement(6965..7468)
                     /gene="cheW"
                     /locus_tag="OO515_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6577038.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7489..9453)
                     /gene="cheA"
                     /locus_tag="OO515_003181"
     CDS             complement(7489..9453)
                     /gene="cheA"
                     /locus_tag="OO515_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6577039.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9458..10384)
                     /gene="motB"
                     /locus_tag="OO515_003182"
     CDS             complement(9458..10384)
                     /gene="motB"
                     /locus_tag="OO515_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6577040.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10381..11268)
                     /gene="motA"
                     /locus_tag="OO515_003183"
     CDS             complement(10381..11268)
                     /gene="motA"
                     /locus_tag="OO515_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6577041.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11394..11972)
                     /gene="flhC"
                     /locus_tag="OO515_003184"
     CDS             complement(11394..11972)
                     /gene="flhC"
                     /locus_tag="OO515_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6577042.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11975..12325)
                     /gene="flhD"
                     /locus_tag="OO515_003185"
     CDS             complement(11975..12325)
                     /gene="flhD"
                     /locus_tag="OO515_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6577043.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2829 a         3449 c         3016 g         3055 t
ORIGIN      
        1 attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag ctgcaaccta
       61 cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata aatactgtga
      121 tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca
      181 aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc acgggaacgc
      241 gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc ttctgttttg
      301 tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt aaatgatgcc
      361 agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt tagggctttt
      421 gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac
      481 cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc aagactatcc
      541 aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt atcgacctga
      601 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
      661 tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat catccgctta
      721 atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
      781 ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc tgttaccagt
      841 tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
      901 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
      961 gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg
     1021 gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg caaactgtcg
     1081 cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
     1141 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
     1201 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc
     1261 ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac
     1321 tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca ggccatccat
     1381 attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
     1441 agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt
     1501 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
     1561 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccaac
     1621 atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc
     1681 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc
     1741 gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt caggatcacc
     1801 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc
     1861 cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc gccactacgc
     1921 gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct
     1981 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
     2041 cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac
     2101 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
     2161 ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa tggcttatgt
     2221 gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc
     2281 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc
     2341 agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc
     2401 attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca
     2461 acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga
     2521 tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
     2581 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
     2641 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt
     2701 tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
     2761 aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag aagatcgcat
     2821 caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca aaatcaacat
     2881 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
     2941 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc
     3001 ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg
     3061 gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg
     3121 aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga tccgcgagca
     3181 gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
     3241 acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat
     3301 ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
     3361 ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
     3421 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
     3481 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
     3541 gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg
     3601 aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct tctaccagcg
     3661 aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc
     3721 cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc acgcgggtca
     3781 ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc gaaccctgct
     3841 ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac
     3901 ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc tgctctccgg
     3961 cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaattccgt
     4021 cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt
     4081 gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca
     4141 gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca
     4201 tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt gagagatcgt
     4261 tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc ttacgcacat
     4321 cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa atggcggtga
     4381 tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca atgctgtcaa
     4441 aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac
     4501 tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc gaaatctgat
     4561 agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc agctgccagg
     4621 cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg aggaaatccg
     4681 aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa
     4741 aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca
     4801 taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg
     4861 ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg ttcagcgcgg
     4921 tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc tgattgctct
     4981 gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac
     5041 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
     5101 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta aatactcgtc
     5161 aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt gccggtggtt
     5221 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
     5281 ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca gcggcggcgg
     5341 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
     5401 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca atctcgccca
     5461 taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
     5521 cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg gctttgatct
     5581 cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc accacggcaa
     5641 aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat
     5701 tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc ttcgaactat
     5761 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
     5821 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
     5881 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
     5941 cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
     6001 catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
     6061 cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta ttcgctaggt
     6121 taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta agcaacatac
     6181 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
     6241 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
     6301 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
     6361 ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc agttcagtta
     6421 acgctgtgta atagttttta tatttttcat caatattacg actggtagcg accatttcag
     6481 gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc aatgttttcc
     6541 tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa tccatcatca
     6601 tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc caggttgacg
     6661 tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc tggctatggt
     6721 gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat acccccagca
     6781 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
     6841 agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca cccgccgcga
     6901 aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga ggaaatcggg
     6961 agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg ctgttcagca
     7021 gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt ccagtgagat
     7081 attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc gccgtcaatg
     7141 aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg agattcagga
     7201 cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta attcgtaagt
     7261 caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt
     7321 tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg atatcaatac
     7381 cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc tcgctggcca
     7441 gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc aggcggcggt
     7501 gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc
     7561 cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc gatagttact
     7621 ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca gcaaggcgta
     7681 gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg ttttcgcgcc
     7741 cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc gcacttccag
     7801 tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca gtgattccat
     7861 aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca tgccgtcgag
     7921 gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct gcttcgactg
     7981 gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca tgccgacgcc
     8041 gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa atatcagcat
     8101 cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc
     8161 cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc
     8221 gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg cggcgagccg
     8281 tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg tcagcgggtc
     8341 gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca gcgtcagttc
     8401 tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc gactaaaaac
     8461 atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac gggcgttacg
     8521 ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca gttcgctgga
     8581 acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa ttaattgatc
     8641 aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg ttttttcccg
     8701 ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg gggtggatat
     8761 ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca caaaacagag
     8821 taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga gcgaatccgc
     8881 ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca gcaggtcgac
     8941 ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc gactctgctc
     9001 atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg gcgtttcgcc
     9061 tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc tggcggcatc
     9121 cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa
     9181 aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt catcgagcag
     9241 gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc
     9301 tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg gcgcttccgg
     9361 ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt catcaaaaaa
     9421 tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg gctgatggca
     9481 ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt actggctcat
     9541 tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca
     9601 ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg
     9661 ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc agttcgcggc
     9721 gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc tcaccgctgg
     9781 cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata ccgttcagta
     9841 caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca ctgccggttc
     9901 taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc tggaccagat
     9961 cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc aactggtcga
    10021 gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga tcttcgatgt
    10081 tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg ccgggaattg
    10141 ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt ggagtccgga
    10201 agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc cacatcacca
    10261 gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac gatccatgtg
    10321 ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct
    10381 tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca catgctcttc
    10441 cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa actcaacggc
    10501 gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc actgcatcat
    10561 tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa taaatccgta
    10621 agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg cacccagctc
    10681 ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag ccgcgacaat
    10741 accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga cttctgcctc
    10801 gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg tgttcatatg
    10861 accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc tatccgcgag
    10921 gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat cacgttccag
    10981 cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag ccagcagatc
    11041 catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg ccttcagcgt
    11101 gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg caccggcaat
    11161 aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca tcaaataacc
    11221 gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga catcatcctt
    11281 ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat tggggcaaaa
    11341 aaagcagcgg tacgtcgtta ccgctactgg aatgttgcgc ctcaccgtat cagttaaaca
    11401 gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt ctgggaaagt
    11461 ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa gctgccaaca
    11521 ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga aagttgcagt
    11581 aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag cagtggtcct
    11641 tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac cgcatcgacg
    11701 ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat cgaagcatga
    11761 acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc tttcggcggt
    11821 gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa ctgtgtttcg
    11881 ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag ctgaatatcc
    11941 cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg cgcagcgctt
    12001 cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg gtatgaattt
    12061 gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg tggctgtcaa
    12121 aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc ggaagagtca
    12181 gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata gcggacgctt
    12241 tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg tcataaatgt
    12301 gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAJTXQ010000116        12167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000116 DAJTXQ010000000
VERSION     DAJTXQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.116"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OO515_003186"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OO515_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6577044.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO515_003187"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO515_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6577045.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO515_003188"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO515_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6577046.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OO515_003189"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OO515_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6577047.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OO515_003190"
     CDS             complement(4036..5568)
                     /locus_tag="OO515_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6577048.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OO515_003191"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OO515_003191"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6577049.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OO515_003192"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OO515_003192"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6577050.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OO515_003193"
     CDS             complement(8663..10399)
                     /locus_tag="OO515_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6577051.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO515_003194"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO515_003194"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6577052.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OO515_003195"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OO515_003195"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6577053.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
BASE COUNT         3110 a         3117 c         3199 g         2741 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagag
//
LOCUS       DAJTXQ010000117        12037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000117 DAJTXQ010000000
VERSION     DAJTXQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.117"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            9..323
                     /locus_tag="OO515_003196"
     CDS             9..323
                     /locus_tag="OO515_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6577054.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="OO515_003197"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="OO515_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="OO515_003198"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="OO515_003198"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6577055.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLSMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="OO515_003199"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="OO515_003199"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6577056.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OO515_003200"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OO515_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6577057.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OO515_003201"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OO515_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6577058.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OO515_003202"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OO515_003202"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6577059.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="OO515_003203"
     CDS             8424..8927
                     /locus_tag="OO515_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6577060.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="OO515_003204"
     CDS             9004..9687
                     /locus_tag="OO515_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6577061.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="OO515_003205"
     CDS             9766..10752
                     /locus_tag="OO515_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6577062.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="OO515_003206"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="OO515_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6577063.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2861 a         3094 c         3225 g         2857 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gtggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtcta tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAJTXQ010000118        11876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000118 DAJTXQ010000000
VERSION     DAJTXQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.118"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1759
                     /locus_tag="OO515_003207"
     CDS             <1..1759
                     /locus_tag="OO515_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6577064.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2095..3072
                     /gene="csiD"
                     /locus_tag="OO515_003208"
     CDS             2095..3072
                     /gene="csiD"
                     /locus_tag="OO515_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6577065.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3092..4360
                     /gene="lhgO"
                     /locus_tag="OO515_003209"
     CDS             3092..4360
                     /gene="lhgO"
                     /locus_tag="OO515_003209"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6577066.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4383..5831
                     /gene="gabD"
                     /locus_tag="OO515_003210"
     CDS             4383..5831
                     /gene="gabD"
                     /locus_tag="OO515_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6577067.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5845..7125
                     /gene="gabT"
                     /locus_tag="OO515_003211"
     CDS             5845..7125
                     /gene="gabT"
                     /locus_tag="OO515_003211"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6577068.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7436..8752
                     /locus_tag="OO515_003212"
     CDS             7436..8752
                     /locus_tag="OO515_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6577069.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8773..9435
                     /gene="csiR"
                     /locus_tag="OO515_003213"
     CDS             8773..9435
                     /gene="csiR"
                     /locus_tag="OO515_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6577070.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9436..9885)
                     /gene="kbp"
                     /locus_tag="OO515_003214"
     CDS             complement(9436..9885)
                     /gene="kbp"
                     /locus_tag="OO515_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6577071.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9969..10127)
                     /locus_tag="OO515_003215"
     CDS             complement(9969..10127)
                     /locus_tag="OO515_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6577072.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10310..10609
                     /locus_tag="OO515_003216"
     CDS             10310..10609
                     /locus_tag="OO515_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6577073.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10619..11143
                     /gene="ygaP"
                     /locus_tag="OO515_003217"
     CDS             10619..11143
                     /gene="ygaP"
                     /locus_tag="OO515_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6577074.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11190..11594)
                     /gene="stpA"
                     /locus_tag="OO515_003218"
     CDS             complement(11190..11594)
                     /gene="stpA"
                     /locus_tag="OO515_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6577075.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2964 a         2928 c         3062 g         2922 t
ORIGIN      
        1 tgtatataat attaacgtta cgtcaccttc cggacagcaa gaaacattta aaatactaat
       61 caatccagaa catctacggc agacacttga gcgtaaatct cttactgctg ttcagaaatc
      121 acaatgtgaa atcatcaccc ctaaaaaacc cggcgaagcg attcttcatg cttttaatgc
      181 cacctaccag caaatcaggg aaaatatgtc tgagtttgca cgttgccatt atgggtatat
      241 acaaatccct ccagtgacaa ctttccgcgc cgacggacca gaaactcccg aagaagaaaa
      301 gggttactgg tttcatgctt atcaacccga agatctttgt accatccata atccaatggg
      361 agatttgcag gattttattg cattggttaa agatgctaaa aaatttggta tcgatatcat
      421 tcctgattat acctttaact ttatgggaat tgggggtagt ggtaaaaatg acctggatta
      481 tccctctgct gatatacgag cgaagatcag taaagatata gaaggtggta tccctggcta
      541 ttggcaaggt caggttttga ttccattcat taaagatcca gtaacaaaag aacgtaaaca
      601 aatccatcca gaagatatac atctcactgc aaaagacttc gaagcaagta aagataacat
      661 ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaattcc
      721 taaaacaaca cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat
      781 gcgaaaatat ggcgtacgag gtttacgtta tgatgcggca aaacactcaa aacatgaaca
      841 aatagaaaga tcaataaccc caccgcttaa aaattataat gagcggttac acaatactaa
      901 cttatttaac ccaaaatatc ataaaaaagc cgttatgaat tacatggaat atctggtaac
      961 ttgtcagttg gatgaacaac aaatgtcatc actgctttat gaaagagatg atttaagcgc
     1021 cattgatttt tcattgctca tgaagacgat aaaagccttt tcattcgggg gagatctcct
     1081 aacccttgca tcaaaaccgg gttccacaat ctcaagcatc ccatcagaaa gacggatatt
     1141 gattaacatt aaccacgatt ttcctaacaa tggcaatctt ttcaatgact ttctatttaa
     1201 ccatcaacaa gatgaacaat tagcaatggc atatatggcc gctctcccgt tcagcagacc
     1261 tttagtttac tgggatggcc aggtattaaa atcaacgact gaaattaaaa attatgatgg
     1321 gtccacgcgt gtcggtggtg aggcgtggct taataaaggt tgctctacct atcagcagct
     1381 ctacaatgaa ttccacgcat tatatataga taaagcagga atatggagcg catttgaggg
     1441 tgtatttgca actaaaaacg ttctagcctt tagtcgtggg gattctgtga acattaatca
     1501 ctctcctcat gatggactag ttataataaa taaaggaaac gaagaagttg aaggtacctg
     1561 gcctaacaaa ttgcaacctg gaatatataa aaacatgggg agtaatagcg ttaacattat
     1621 tattaataat acccgaaaaa ttatcccccc tggtaaagta tttacgctta gaggcggaac
     1681 tctaaatatc aatattcctg ggcgtagcgc tcttctttta gggaaaacag gagaaccgcc
     1741 gaactatctc tatttataat ttaatgttat atctgccccg ataaaacggg gcagataata
     1801 tgtttagttt actaacggtc attttgcagt gaagccattt actgtttttt atcgaccaga
     1861 taatctgttc tctaatgtta actcccccta acctgttgct ttagttattc atttcctgtc
     1921 tcactttgcc ttaatactct acgttaaatg ttactaattt gttgcttttg atcacaataa
     1981 gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt tagattatgg
     2041 ctacgaaatg agcatcgcca tgtcacccta catctcataa gaggatcgct tctgatgaat
     2101 gcactgaccg ccgtacaaaa taacgctgtc gattcaggcc aggactatag cggattcact
     2161 ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca ccttcaccga acagacgacc
     2221 aaacagtttc tcgagcaggt tgccgaatgg cccgtacagg cgctggagta caaatcgttt
     2281 ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg cgaatcagct gcaaccattg
     2341 ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc ggtgggtatc
     2401 gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa cggcggtagc gcatctgatt
     2461 ggtcgctcaa atttcgatgc gatgagcggt cagtattacg cgcgtttcgt ggtgaaaaat
     2521 gtcgataact cagacagcta tctgcgtcag ccgcaccgcg tgatggaact gcacaacgac
     2581 ggcacgtatg tcgaagagat caccgattac gtgctgatga tgaaaatcga tgagcaaaac
     2641 atgcagggtg gaaattcgtt gctgctgcat ctcgatgact gggaacatct ggaccactat
     2701 ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc
     2761 agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc
     2821 tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt
     2881 tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt tggcaaattc
     2941 ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat
     3001 ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca ccactaccag
     3061 acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta ttggcggcgg
     3121 catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg cccgcattgc
     3181 gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca gcggcgtgat
     3241 ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc tggcggggaa
     3301 ccgcgccacc aaagcctttt gcgatcaaaa cggcattcgc tatgacaact gcggcaagat
     3361 gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc gccttatggg aacgcacagc
     3421 ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa
     3481 tatcaccggg cttggtggca tttttgtgcc gtccagcggc attgtcagct accgcgaagt
     3541 cacagcggca atggcaaaaa tcttccaggc cagaggcggc gagattattt ataacgccga
     3601 agtcagcgcc ctcagtaagc ataaaaacgg cgtggtgata cgcacccgtc agggcggtga
     3661 atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc tggtgaaaat
     3721 gctcggcctc gaaccgggct ttatcatctg cccgttccgt ggtgagtatt tccgccttgc
     3781 gccggagcat aaccagattg ttaaccacct gatttacccc attcccgacc ccgcgatgcc
     3841 atttttgggc gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc
     3901 ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta gtgacacgct
     3961 ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac gctcaggact
     4021 gggcgagatg aaaaactcgc tgtgcaaaag cggctacctg cggctggtgc aaaagtattg
     4081 tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt
     4141 atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac gcacgatcca
     4201 cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg cgcatattgt
     4261 cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc
     4321 acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa caggacgtaa
     4381 caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac ggggaatggc
     4441 tggacgccaa caatggcggg atcatcgacg tcaccaatcc ggcgaacggc gacaagctgg
     4501 gtagcgtgcc gaaaatgggc gctgatgaaa cccgcgccgc tatcgacgcc gccaaccgcg
     4561 ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caacattctg cgcaactggt
     4621 tcaatttgat gatggagcat caggacgatt tagcgcgcct gatgaccctc gaacagggca
     4681 aaccactggc cgaagcgaaa ggcgaaatca gctacgctgc ctcttttatt gagtggtttg
     4741 ccgaagaagg caaacgtatt tatggcgata ccattcccgg tcatcaggcc gataagcgcc
     4801 tgattgttat caagcagccg attggcgtta ccgcagctat cacgccgtgg aacttcccgg
     4861 cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc atggtgctga
     4921 agcccgccag tcagacaccg ttctctgcgc tggcgctggc ggagctggcg attcgcgcgg
     4981 gcattccggc tggcgtgttt aacgtagtca ccggttcggc aggcgcggtc ggtaatgaac
     5041 tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttcgaccgaa attggccgcc
     5101 agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg ggcggcaacg
     5161 cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc gcgctggcct
     5221 cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg
     5281 gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt gagcaaactg cacatcggcg
     5341 acgggctgga taaaggcgtc accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag
     5401 tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag
     5461 cggatgaacg cggcggcaac ttcttccagc cgaccattct ggtggacgtg ccggccaacg
     5521 ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc gctgttccgc tttaaagatg
     5581 aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg ccttgccgcc tatttctacg
     5641 cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc atcgtcggca
     5701 tcaataccgg cattatttcc aatgaagtgg ccccgttcgg cggcatcaaa gcctcgggtc
     5761 tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa tatatgtgca
     5821 tcggtcttta actggagaat gcgaatgaac agcaataaag agttaatgca gcgccgcagt
     5881 caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac
     5941 tgccgggtgt gggacgttga aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg
     6001 ctcaataccg ggcacctgca tccgaaggtg gtggccgcag tggaagcgca gttgaaaaaa
     6061 ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct gtgcgagatt
     6121 atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac gaccggttcc
     6181 gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc
     6241 gcttttagcg gcgcgtatca cgggcgcacg cattacacgc tggcgctgac cggcaaggtg
     6301 aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc gctttatcct
     6361 tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg gatcttcaaa
     6421 aatgatgccg cgccggaaga tatcgccgcc atcgtgattg agccggttca gggcgaaggc
     6481 ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg tgacgagcac
     6541 gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt
     6601 gcgatggagc agatgggcgt ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc
     6661 ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga tggacgctgt tgcaccgggc
     6721 ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggctgcgct ggaagtgttg
     6781 aaggtgttcg agcatgaaaa tctgctgcaa aaagccaacg atctggggca gaagctaaaa
     6841 gacggattgc tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg cgggctggga
     6901 gcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc
     6961 accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc ctgcggcccg
     7021 tattacaacg tgctgcgcat ccttgtaccg ctcaccattg aagacgctca gatccgtcag
     7081 ggtctggaga tcatcagcca gtgttttgct gaggcgaagc agtagctatg tccgtttgta
     7141 agagttaacc gttgctaagg gaatgataac gatgcagtaa aaaataataa cgatgaggcg
     7201 aacgacaaag ttaaaaacga ctgcggacag tcccctcgcc cctctggaga gagggttagg
     7261 gtgaggggta aaacacgcgg ctaaaccctc ccctggccct ctccctaagg gagagggaga
     7321 aaaccctccc gctttcgttt ttaagatttt gttgaccgtc tcaattaaac gcgtgacccg
     7381 gcgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat tcaggatggg
     7441 gcaatcatcg caaccacatg agttaggcgg cgggctgaaa tcacgccacg tcaccatgtt
     7501 gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga
     7561 agcgggcccg gcggtattac tggcctatct gttcgccggg ctactggtgg ttatgattat
     7621 gcggatgttg gcggaaatgg cggttgccac gcccgatacc ggttcgtttt ccacctatgc
     7681 cgataaagcc attggtcgct gggcgggcta taccatcggc tggttgtact ggtggttttg
     7741 ggtactggtt atcccgctgg aagccaatat cgccgctatg atcctgcact cgtgggttcc
     7801 aggcattccc atctggttat tttccctcgt cattaccctt gccttaactg gcagtaattt
     7861 attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca aagtcatcgc
     7921 tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc cttatgccga
     7981 agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg gtttcggtgc
     8041 ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat
     8101 tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgccacca actcggttat
     8161 ctggcgtatt tctatcttct atttgtgctc tatttttgtc gtcgtggcat taataccgtg
     8221 gaatatgccg gggctgaaag ccgttggatc ttatcgctcg gccctgtata ccgcctcaag
     8281 gatgctctac tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg
     8341 cagtaaaacc ccgtacgtgg cggtgttact ctccaccggc gcggcattct taacggtggt
     8401 ggtgaactat tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat
     8461 cgccctgctg gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg
     8521 agcggaagga agcgaaattc gcttgcgaat gtggctttat ccctggctca cctggctggt
     8581 catcggcttt attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga
     8641 agtgatctcc accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg
     8701 ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc
     8761 aggagtcaca ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt
     8821 attcgcggta attttcaacc ggatgaaaaa ttacggatga gtttgctgac atcgcgttat
     8881 gcacttggcg ttgggccgtt gcgggaagca ctttctcaac tggtggcgga acggctggtc
     8941 acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat
     9001 attttcgatg cccgcgccaa tatggaagcg atgttagtga gtctggcgat tgcccgcggt
     9061 ggcgatgagt gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc
     9121 tgtgacgcca gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca
     9181 gcaattgtgg cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat
     9241 ctggcggcgc gctatcgatt tatctggctg cggcgaacgg tgctttcggt agaaatgctg
     9301 gaggataaac acgatcagca ccagaccctg actgcggcgg tactggcgcg agataccgcg
     9361 cgcgccagtg agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg
     9421 atggctggca attaactact cttccggaat acgcaacact tgccccggat aaattttatc
     9481 cgggcttttt agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc
     9541 gtagacctgt ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct
     9601 ggcagtggct ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt
     9661 ccccaccgca acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt
     9721 cgctttgccg tcggcaattt gaatgttcac tttatcagca tcaggtatac cggttttgct
     9781 cagatgctcc tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc
     9841 ccagagtttt tctccggcat ctttcacaaa attgaacaga cccatagcta cctcattcgt
     9901 taacggaaac actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga
     9961 gcacaggttt aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca
    10021 agcagtgtca gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg
    10081 ccgagcggcg gtagaatgat ggtgatgacg attctccaga aacccatatg tactccctat
    10141 aagaaaatta ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg
    10201 agcctgccag tttcagctta cgcttttact ctcatttacg ctgctgaggc tggtatatgt
    10261 tgcattttta gtttagagct tacttaattt agaaaatact taaataaata tgactgaact
    10321 cgcgcaatta caggccagtg ccgaacaggc agcggcctta ttgaaagcaa tgagccatcc
    10381 taaacgattg ctgattctgt gcatgcttag cggttccccc ggcaccagcg cgggagagct
    10441 gacgcgcatt accggactga gtgcctctgc gacatcacag catctcgctc gtatgcggga
    10501 cgaagggctt atcgacagcc aacgggatgc ccaacgcatt ctatattcca ttaaaaatga
    10561 ggcggtaaat gccattatcg ccaccctgaa aaatgtctat tgtccgtaag gagtcactat
    10621 ggctttgaca accatttcgc cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt
    10681 aatcgatatt cgtgacgctg atgaatatct tcgtgaacat attcctgaag cagatctggc
    10741 tccattatcc gtgctggaac agtcaggtct tccacctaaa ttacgtcgcg agcaaattat
    10801 attccactgc caggcaggta aacgcaccag taataacgcc gataaattag cagcgattgc
    10861 cgcccccgca gaaatatttt tactcgaaga tgggattgat ggctggaaaa gagctggatt
    10921 gccagtagcg gtaaataaat ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc
    10981 gggtggttta atattaatcg gcgttgtact gggttatacc gtaaatagcg gtttcttctt
    11041 attaagtggc tttgttggtg ccgggttact gtttgcagga atcagcggtt tttgtggaat
    11101 ggcaaggttg ttagataaga tgccgtggaa ccaacgagct tgagaagcga cgccggacgc
    11161 gccctagcag cgacatccgg cctcagtaat tagatcagga aatcgtcgag agatttacct
    11221 tctgccagcg cctgagcaat tggcttcggt gtacggccct gaccggtcca ggttttagtt
    11281 tcaccgttaa catcggtgaa tttatatttc gccggacgcg gctggcgttt tttaccagcg
    11341 cgtggtgcag cagcagagct attacccaat aactcttccg ggttaattcc gtcagccttc
    11401 atcagctcca gccaggtgct aattttttcc tgacgctcag ccagttcacg ctgctgctgt
    11461 tcttcttctt cacgtctttc tttagtgaca accctgaatt tttcgagcat ttcttcaaga
    11521 acgtcaatgg agaattcgcg agccatcgca cggagggtgc gaatgttatt taaactttgt
    11581 aacattacgg acataataaa gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc
    11641 gtagtgtaag cgatcactta ttatatttcc acaaaattta atctatttac agtcttgcgc
    11701 gagattattt cagctgttat tttctgaaac tctcagaata atttcaagat tatatattag
    11761 ccattttgat gagtaattta gattattggt gaacagaacg gcgctctctg ctcgctaatt
    11821 ccgcaagcct gacaaaaccg aagtgaataa aaatgcaata taacagaata aaagaa
//
LOCUS       DAJTXQ010000119        11809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000119 DAJTXQ010000000
VERSION     DAJTXQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.119"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..488
                     /locus_tag="OO515_003219"
     CDS             <1..488
                     /locus_tag="OO515_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6577076.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="OO515_003220"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="OO515_003220"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6577077.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OO515_003221"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OO515_003221"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6577078.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OO515_003222"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OO515_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6577079.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="OO515_003223"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="OO515_003223"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6577080.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OO515_003224"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OO515_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6577081.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="OO515_003225"
     CDS             complement(6839..7747)
                     /locus_tag="OO515_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577082.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="OO515_003226"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="OO515_003226"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR6577083.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="OO515_003227"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="OO515_003227"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6577084.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11809
                     /locus_tag="OO515_003228"
     CDS             10715..>11809
                     /locus_tag="OO515_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6577085.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
BASE COUNT         3006 a         2999 c         2927 g         2877 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctacctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagttaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgct
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatg
//
LOCUS       DAJTXQ010000120        11731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000120 DAJTXQ010000000
VERSION     DAJTXQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.120"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..68
                     /locus_tag="OO515_003229"
     CDS             <1..68
                     /locus_tag="OO515_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6577086.1"
                     /translation="GTNTLLACCCLLRPEEVNSEY"
     gene            58..231
                     /locus_tag="OO515_003230"
     CDS             58..231
                     /locus_tag="OO515_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6577087.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(280..1353)
                     /locus_tag="OO515_003231"
                     /pseudo
     CDS             complement(280..1353)
                     /locus_tag="OO515_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1425..4169)
                     /gene="torS"
                     /locus_tag="OO515_003232"
     CDS             complement(1425..4169)
                     /gene="torS"
                     /locus_tag="OO515_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6577088.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4252..5280
                     /gene="torT"
                     /locus_tag="OO515_003233"
     CDS             4252..5280
                     /gene="torT"
                     /locus_tag="OO515_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6577089.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5253..5945)
                     /gene="torR"
                     /locus_tag="OO515_003234"
     CDS             complement(5253..5945)
                     /gene="torR"
                     /locus_tag="OO515_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6577090.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6065..7237
                     /gene="torC"
                     /locus_tag="OO515_003235"
     CDS             6065..7237
                     /gene="torC"
                     /locus_tag="OO515_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6577091.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7237..9648
                     /gene="torA"
                     /locus_tag="OO515_003236"
                     /pseudo
     CDS             7237..9648
                     /gene="torA"
                     /locus_tag="OO515_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(10287..10592)
                     /gene="cbpM"
                     /locus_tag="OO515_003237"
     CDS             complement(10287..10592)
                     /gene="cbpM"
                     /locus_tag="OO515_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6577092.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10592..11512)
                     /gene="cbpA"
                     /locus_tag="OO515_003238"
     CDS             complement(10592..11512)
                     /gene="cbpA"
                     /locus_tag="OO515_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6577093.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2805 a         3038 c         3112 g         2776 t
ORIGIN      
        1 cgggaaccaa caccttactg gcgtgttgct gtcttttaag accagaagag gttaacagtg
       61 aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa
      121 atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt caccgcacga
      181 gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa
      241 catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
      301 ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata ctcccgcata
      361 cattgcgtaa ccaatcaccg cgaacataat cctcagaacc agtagcgtca ttaaccagcc
      421 agacaacgca aaagcttttt tatttgccgc aggtttttgc agtgaaaagg tcaatgttga
      481 ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
      541 aattaattta tctaccggga tccgtgatgg gcaatttttt gcgcatttgc cacagccgat
      601 acaactttcg gcattgcgac gaatcttaaa cggcgataat agcgaaacca cgcccatcag
      661 cgcgccatat gggcaaagat aacgacacta ggcatggcga ataaacaggc tggcaatcag
      721 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
      781 tacgtccatc accacgctgt agggcgacaa cataaaatag tgaatcgcct gagcgggcat
      841 caataacgcg atatagagaa aaaaactcaa cagcaaatac ttcacgccgc gcagaggaat
      901 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
      961 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
     1021 actgatgacg ataaaagcga ccaataacag catcgcggcg gtgtggacgg acggtaactg
     1081 acctgttgta aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat
     1141 acctcccggt ctggcgacaa atgtcgtgct acttgccgtt tcgtaatagc gcacccaata
     1201 ccagaacgtg atggcaatat aaatattcat tgccaacagt aataattgcg tcgctttacg
     1261 ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg
     1321 ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata
     1381 ccaaatgcca ctattctagt tgttcttaac tggctgatat tgattcaaat cgcgttcagg
     1441 tctttcttat gcaaccatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtgga
     1501 aggggggcag ataatggctg ctgctccagt tgtgcgcata gctggctggc gatacgcatt
     1561 cccaaactca agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctatcc
     1621 tgactggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tgtaaataat
     1681 accagccatt cgtggatctt ctccgtcccc attaactgag catcttcatt gagttgcgat
     1741 acatccagcg attgatcgtt attgacttgc agttggagat agtgcgccag taactgaccg
     1801 agcacttcac gcggcaccgg tttagggata atcccgcgga ataatgaact ggtacgctgg
     1861 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
     1921 tgtgccagtt gtcgggcaag cgtaatgccg tcgacatccg gcagatcaaa atccaccagt
     1981 gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaaca
     2041 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
     2101 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt gactgttttt
     2161 ggcacgggtg ccgtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
     2221 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
     2281 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
     2341 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
     2401 ccatcagtgc gactacgcag gataatgtac ccttcgtcag taaaacgcag ggcgttgctc
     2461 aacaggttgg ttataacctg acgaatacgt cgtggatcgc ccattaacgc gcacggcata
     2521 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
     2581 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac cgaaacattc
     2641 ttgccacctg cttcgatagc ggaataatcg agaatatcgt tgaggatggt cagcaacgat
     2701 tcaccagagt cagtaattgc ccgcaaatca tcacgctggg cgttaagtgc ggggttatct
     2761 gccagtagct gtgcggtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
     2821 gccgccagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
     2881 tgttctatca ctagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
     2941 tcacggtggc gattcagtgc atgaacactg ctgcgaaacg cacccatcag ctgcccgatg
     3001 gtgtccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
     3061 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgactgagcg ataaaccacg
     3121 cgccagagga tcagaatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat
     3181 tgcccgcgtg cactggcttt ttcctgatgc gccagtccgt gctgattacg cagctcaatg
     3241 gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat attattttgg
     3301 gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc caacagatcg
     3361 ctatattggc taacggtagt taacgttgtt gcgacctgcg cacgaactcc cggatcttca
     3421 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
     3481 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
     3541 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
     3601 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
     3661 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgcgccag gcgtgcgatc
     3721 tcatcggcgg cagcgactat ctgctgactg agttgctgtt gttgctggcg tagttgcaga
     3781 cgccgcccca ccagttcccc ttgctgacgt aacgaacggg agatctcctg ctcctgttgt
     3841 tcaatagcgg tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc attaatcttc
     3901 aggctttgtg cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt atcggcactg
     3961 gtcaggttct gcgcggcgaa aagttcccag gcgctggctt cgctcaactg gcgcgccata
     4021 ttcatggtag gaatcaatgc ctgagtattg tctttttcca cctggctgat aaagcgcagg
     4081 ttgtaccatc ccaccagggt actggtcagg gttaacagcg ccatcagggc aaagcccatc
     4141 cagagtcttc gggtcagggt taaattcacg gtcggtgcac tttaggtgaa aaatgttgag
     4201 tcgcaaagcg gaatgcatct agcataaagc cttattattg atgaggctat catgcgcgta
     4261 ctgctatttt tacttctttc ccttttcatg ttgccggcat tttcggctga taacctgttg
     4321 cgctggcatg atgcgcagca tttcacggtg caagcctcta tgccgcttaa agccaaacgc
     4381 gcatggaaac tgtgcgcgct ttatcccagc ctgaaagatt catactggtt atcgttgaac
     4441 tatggtatgc aggaggcagc tcgccgttat ggtgtggatt taaaagtgct ggaggcaggc
     4501 ggctacagcc agttggctac ccagcaagca caaatcgacc agtgtaaaca gtggggcgca
     4561 gaggccattt tactcggtag tagcacgacc tcatttcccg atctgcaaaa gcaggtagca
     4621 agtctgccgg tgatcgaact ggtaaatgct attgatgctc cccaggtgaa aagccgcgtt
     4681 ggtgtaccct ggtttcagat gggctatcaa cctgggcgat atttggtgca atgggcgcac
     4741 ggtaaaccac tgaacgtgct gttgatgccc ggacccgata atgccggggg cagtaaagag
     4801 atggtcgagg gttttcgcgc agccattgcc ggaagcccgg tgcgtattgt tgatattgcg
     4861 cttggtgata acgatattga aatccagcgt aacctgttgc aggagatgct ggaacgccac
     4921 ccagaaatcg acgtcgttgc cggaacggcc attgcggcag aggcggcaat gggtgaaggg
     4981 cgtaacctga aaacgccgct taccgtggtg tcgttttatc tttcacatca ggtgtatcgc
     5041 gggctgaagc ggggaagagt gattatggct gccagcgatc aaatggtctg gcagggggaa
     5101 ctggcggttg agcaggccat caggcaatta caggggcaat cggtgtctga taatgtcagc
     5161 ccaccgattt tagttctgac gccgaaaaat gccgaccgtg aacatattcg ccgctcgctg
     5221 tcaccagggg gatttcgtcc ggtctatttt tatcagcaca catcagcggc taagaaataa
     5281 ccttcaccat gttgcgtcac cagtaaatcc gcgctgagtt tatgacgtaa acgacgaatt
     5341 aacacatcga cggtgcgcag gtcagggttt tccacccgac gcgcagaaag catacgtagc
     5401 agacgttcac ggctgagaat ttcgcccgga ttcgtcacaa atgccaccaa catttcatac
     5461 tctgcgcggg tcagtttaat cggctcgcca tcccgctcca gcgtatggcg cgacacattc
     5521 aggcaataac cggcaaagcg atagcagttg tcctgagtgt gcggttgagc ttgtcgcgcg
     5581 aggtcgattc gccagagcag atttttcacc cgtactacca gttcgcgcag ttccagcggt
     5641 ttggtgacgt aatcgtctgc gcccatttcc agcccaacaa tacggtcaat ccgatcgctg
     5701 cgtccggtaa ccagaataat ccccaccgtt gagcgttctc gcagggcgcg ggttaacatc
     5761 aggccatttt catcgggtaa gttgatatcc agcagaatta aatctaccgg ctgattctgc
     5821 ataatttccc gcagcccggc accgctcgct gtaacggaaa cggtataccc ctcctgagtg
     5881 aagtaagatt gtaatcgcgc ctgggtaacc ggctcatctt caacaataac aatgtgatgt
     5941 ggcatcagag ggttttactc attctgttca tattctgccg taagccgttc atcctgacca
     6001 gtgccgctgt tcatatttgc tcattaagat cgcttcacta aaccataatt ttacaggggc
     6061 tattatgcgg aaactctgga acgcgctacg ccgacccagt gctcgttggt cggtactggc
     6121 gctggtcgct attgggattg tgattggcat tgcgctgatt gtattgccac acgttgggat
     6181 caaagtcacc agcacaaccg aattttgtgt cagttgccac agtatgcaac cggtgtatga
     6241 agaatataaa cagtcggtgc attttcagaa cgcctctggc gtgcgagctg aatgccatga
     6301 ctgtcatatc ccgccggata ttccaggcat ggtgaagcgc aaactggaag cgagcaacga
     6361 catttaccag acctttattg cccactccat tgatacacct gaaaaattcg aagccaaacg
     6421 cgcggaactt gccgagcgtg aatgggcgcg aatgaaagaa aacaactcgg caacctgtcg
     6481 ctcctgccat aactacgatg cgatggatca tgccaggcag catcctgaag cggcacgtca
     6541 gatgaaggtg gcagcgaaag ataatcaatc ctgcatcgac tgtcataaag gtattgccca
     6601 ccagttaccg gatatgagta gcggcttccg taagcagttc gatgagctgc gcgccagtgc
     6661 taatgacagt ggtgacacgc tgtactctat tgatattaag ccaatttatg cggcgaaagg
     6721 cgataaagaa gcctctggtt ctctgctgcc tgcttcggaa gtgaaagtcc ttaaacgtga
     6781 cggcgactgg ctgcaaattg aaattaccgg ctggacggaa agcgccggac gtcagcgtgt
     6841 actcacccaa ttcccaggta aacgcatctt tgttgcctcg attcgtggtg atgtgcagca
     6901 gcaggtgaaa acgctggaga aaaccaccgt tgccgacacc aataccgagt ggagcaagtt
     6961 gcaggccacc gcgtggatga agaaaggcga catggtgaac gatatcaaac cgatctgggc
     7021 ctatgcggat tcgttgtaca acggcacctg taaccagtgc cacggcgcac cggaaatcgc
     7081 ccactttgac gctaacggtt ggatcggcac gctcaacggt atgattggct ttaccagcct
     7141 cgataaacgt gaagaacgca ccttgttgaa atatctgcaa atgaatgcgt ctgacaccgc
     7201 aggtaaggct cacggcgata agaaggacga aaaataatga acaataacga tctctttcag
     7261 gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg gatgctgggg
     7321 ccgtcattgt taacgccgcg ccgtgcgact gcggcgcaag cggcgactga ggctgtcatc
     7381 tcgaaagagg gcattcttac cgggtcgcac tggggggcta tccgcgcgac ggtgaaggat
     7441 ggtcgctttg tggcggcaaa accgttcgaa ctggataaat atccgtcgaa aatgattgcc
     7501 ggattgccgg atcacgtaca caacgcggcg cgtattcgtt atccgatggt acgcgtggac
     7561 tggctgcgta agcgccatct cagcgatacc tcccagcgcg gtgataaccg ttttgtgcgc
     7621 gtgagctggg atgaagccct cgacatgttc tatgaagaac tggaacgcgt acagaaaact
     7681 cacgggccga gtgccttgct gaccgccagt ggttggcaat cgacggggat gttccataac
     7741 gcttcgggga tgctggcgaa agctattgcc ttgcatggta atagcgttgg tacgggcgga
     7801 gattactcta ccggtgctgc gcaggtgatc ctgccgcgcg tggttggctc aatggaagtg
     7861 tatgaacagc aaacctcctg gccgctggta ttgcagaaca gcaaaaccat tgtgctgtgg
     7921 ggctctgatt tgctgaaaaa ccagcaagcg aactggtggt gcccggatca cgatgtttat
     7981 gaatattacg cgcagctaaa agcgaaagtc gccgccggtg aaattgaggt catcagcatc
     8041 gatccggttg tcacatccac ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg
     8101 gttaacccgc aaactgatgt gccgctgcaa ctggcgctgg cgtatacgct gtacagtgaa
     8161 aacctgtacg acaaaaactt cctcgctaac tactgtgtgg gttttgagca gttcctgccg
     8221 tacctgctgg gtgagaaaga cggtcagccg aaagatgccg cataggctga aaaactgacc
     8281 ggcattgatg ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa cagaacgcaa
     8341 attattgctg gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc gtggatgatt
     8401 gtggttctgg cggcgatgct ggggcaaatt ggcctgccag gtggtggctt tggttttggc
     8461 tggcactata acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc
     8521 ggctctacgt cgattccgcc tgttcacgac aacagtgatt acaaaggcta cagcagcacc
     8581 attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac
     8641 ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgctggaac taacccattc
     8701 catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc
     8761 gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc
     8821 acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc
     8881 gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc
     8941 gagctgtgcc gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc
     9001 tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg
     9061 ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg
     9121 tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt
     9181 ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg
     9241 catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat
     9301 ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagtt atgtgagtcg
     9361 gaaactctgc gtcagcaata tacggtagcg ggtaaagagc cagtattcat taacccgcag
     9421 gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt
     9481 caggtgttgg caggggcagt ggtttctgac cgctatgcac ccggcgtggc acgaattcac
     9541 gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt
     9601 aaccccaacg tgttgaccat cgacatcggt acatcgcagc tggcgcagtt gttctcccgt
     9661 gaactggacg atgaacaact gacgcaaatc gccagtgcgc agatggctga atggttttcg
     9721 ttgctgaaaa gcgaaccgcc gctcactgcg gcggtgaatg cgctggaaaa ccgtattgcc
     9781 gctctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
     9841 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
     9901 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaatcg
     9961 gcagatcatc tggcgatcta tctcgagttg ttgagtcatc tgcatttttc gctgggagag
    10021 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
    10081 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
    10141 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
    10201 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
    10261 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
    10321 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
    10381 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
    10441 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
    10501 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
    10561 gtaatagtaa aagtcaccgt aacattagcc atcatgcttt cccccaatct ttacgtggat
    10621 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
    10681 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
    10741 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
    10801 aaatgctttc tttcagtgtt ggaacggtga ctttagcacc cagcgccgct tcccacgggc
    10861 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
    10921 tatgaatcac cagccacaaa tcgccatttg gaccaccgtt ttcgcccggc gtcccctggc
    10981 ctttcagacg gatgcgttga ccattgccga cgcccgccgg gatcttcaca ttcagcgttt
    11041 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
    11101 tacgcttatg ctcagtcagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
    11161 ggccgcgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
    11221 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgatggt
    11281 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
    11341 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
    11401 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
    11461 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
    11521 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
    11581 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
    11641 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
    11701 tttgatagtt gaaccaggca ctttaagttt a
//
LOCUS       DAJTXQ010000121        11542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000121 DAJTXQ010000000
VERSION     DAJTXQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.121"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            460..963
                     /locus_tag="OO515_003239"
     CDS             460..963
                     /locus_tag="OO515_003239"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6577094.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1336..2529)
                     /locus_tag="OO515_003240"
     CDS             complement(1336..2529)
                     /locus_tag="OO515_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6577095.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="OO515_003241"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="OO515_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6577096.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="OO515_003242"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="OO515_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6577097.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="OO515_003243"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="OO515_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6577098.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="OO515_003244"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="OO515_003244"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6577099.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="OO515_003245"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="OO515_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6577100.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="OO515_003246"
     CDS             complement(11165..11308)
                     /locus_tag="OO515_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577101.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2927 a         2896 c         2949 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tagcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAJTXQ010000122        11427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000122 DAJTXQ010000000
VERSION     DAJTXQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.122"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(43..384)
                     /gene="bamE"
                     /locus_tag="OO515_003247"
     CDS             complement(43..384)
                     /gene="bamE"
                     /locus_tag="OO515_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6577102.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(533..2194)
                     /gene="recN"
                     /locus_tag="OO515_003248"
     CDS             complement(533..2194)
                     /gene="recN"
                     /locus_tag="OO515_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6577103.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2280..3158)
                     /gene="nadK"
                     /locus_tag="OO515_003249"
     CDS             complement(2280..3158)
                     /gene="nadK"
                     /locus_tag="OO515_003249"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6577104.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3281..3874
                     /gene="grpE"
                     /locus_tag="OO515_003250"
     CDS             3281..3874
                     /gene="grpE"
                     /locus_tag="OO515_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6577105.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3929..5215)
                     /locus_tag="OO515_003251"
     CDS             complement(3929..5215)
                     /locus_tag="OO515_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6577106.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5236..6027)
                     /locus_tag="OO515_003252"
     CDS             complement(5236..6027)
                     /locus_tag="OO515_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6577107.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6194..7555
                     /gene="ffh"
                     /locus_tag="OO515_003253"
     CDS             6194..7555
                     /gene="ffh"
                     /locus_tag="OO515_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6577108.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7804..8052
                     /gene="rpsP"
                     /locus_tag="OO515_003254"
     CDS             7804..8052
                     /gene="rpsP"
                     /locus_tag="OO515_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6577109.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8071..8619
                     /gene="rimM"
                     /locus_tag="OO515_003255"
     CDS             8071..8619
                     /gene="rimM"
                     /locus_tag="OO515_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6577110.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8650..9417
                     /gene="trmD"
                     /locus_tag="OO515_003256"
     CDS             8650..9417
                     /gene="trmD"
                     /locus_tag="OO515_003256"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6577111.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9459..9806
                     /gene="rplS"
                     /locus_tag="OO515_003257"
     CDS             9459..9806
                     /gene="rplS"
                     /locus_tag="OO515_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6577112.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9883..10365)
                     /locus_tag="OO515_003258"
     CDS             complement(9883..10365)
                     /locus_tag="OO515_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6577113.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10381..>11427)
                     /gene="dgcN"
                     /locus_tag="OO515_003259"
     CDS             complement(10381..>11427)
                     /gene="dgcN"
                     /locus_tag="OO515_003259"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6577114.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2747 c         3061 g         2817 t
ORIGIN      
        1 cattgagcac cttttttaac gtctttgaga gcaactttat tattagttac cactcagcgc
       61 aggtttgtta tcaatattgg tcaacacacc gctactgtta aaggtcagcg tcagcgtttg
      121 ctgcgttaca ccttcatgac ctggttgctg gcggaagaca tagaaccagg tattcgtacc
      181 aaatggatcg gacatcagcg gtgtacccaa tgcgtacgca acttgttgtt gcgtcatgcc
      241 aacacgtatt ttggatacgt cgttagcggt cagatagttc ccctggttga tgtcaggacg
      301 gtaaaccact cgctccagag tggaacagcc tgcggtcaac atcaatagta ctgctgctgc
      361 agcagtcagc gttttacagc gcatagtgat ttgattcctt ttcgggcccg agcagtacgt
      421 ggctcatctg taatatgccg atgataatag acctttcatc actttaaaac cttttgcttt
      481 ccggtcttac ggcgttttgc tgtttactct aaccgtgaag caggaaaaaa gtttacgctg
      541 caagcagttc tttcgcattc gctagtgtat tacgtgtgac ttcactgcca ccaagcaggc
      601 gcgccagctc ttgtaaccgc gcttttttat tcagggattg catatgcgtt tctgtcatcg
      661 caccatcggt ttctttgctg acaaaatagt gttgatgacc acatcccgcg acttgtggca
      721 ggtgggtaac acacatcacc tgagttgatt cgccgagttg acgcagcagt ttgccgacaa
      781 ctgccgctgt tggaccgcta atccctacat ccacttcatc aaaaatcagt gccggggttt
      841 ccattttacg cgccgtgatg acctggattg ccagtgcgat gcgggacaat tcaccaccgg
      901 atgcgacttt ggcaataggc tgcattggct gacctgggtt ggtggttacc cgaaactcaa
      961 tacgatcggc accgtcagcg cccaggtgat gctcgtcaaa tttaacatcg atcgtaaact
     1021 gcccatgcgg cattgagagc gcatgcatac tgtcggtgat cagctgtgca agttcttctg
     1081 catattgctg gcgttgttgg tgtaatgcgc gcgcgatttc cagtgcctgc tgatgatgtt
     1141 tcgttaccgc cagcgcaagc gtttcttgtg agtcggcctg atcgtccagt tgctgctgtt
     1201 cttccagtag cgactggtaa tactgtggca atgcctcagg gctgacgtga tgtttacgtg
     1261 ccagcgaaat ctgttttgag atgcgctgtt caagttcaaa tagtcggttg ggatcgagat
     1321 ccagacgatc gcagtagtgg cgcagttcat cgctggcttc agcaatctgg atggtagctt
     1381 cttccagcat atcaagtacg ccggacagtt tgctgtccat gccaatcaat tcgctcacca
     1441 gttgtttagc cgtgtaaagc tgactttgca ggtttgcgtc ttcaccgtcg gccattaatg
     1501 ccaatgcatt ctggctggtg gtcagcaatt gaccgctgtt cgccagacgt ttgtactctt
     1561 cgtcgatttg ttcaaactct ccgggctgcg gattaaattc gttaagttct tttaattggt
     1621 attgcagcag ttccgcacgg gcggcgcgtt cctgacttaa ctgttgatga tgcgcgaggt
     1681 cacggcagct ttgatgccac aactgataac gtgcggtcat ttcctgcagt agagaggttt
     1741 cattggcata gccatcaagc aggaattttt ggtgctcagg tttggtgagt aattgatgag
     1801 cgtgctgacc atggatctga atcagcaact gacccagttc gcgcagttgt gacagaggaa
     1861 cagctgtacc gttgatgaaa ccacgggagc gaccatcgct gctgatcacg cgacgaagca
     1921 aacattcatg cccgtcttca agctggtttt cttccagcca gcgcagagcc gctggcgtat
     1981 ctttcagaga aaaacgggcg cacaggtcag cgcgagcagc gccggtacgc accatgtcgg
     2041 cttcagcgcg accaccgaga caaagaccga gggcatctat tgcaatagat ttacccgcgc
     2101 cggtctcgcc agttattacg gtcatgccgc tatgaaaatc aatctcaagc tcacgaacga
     2161 tagcaaagtt gctgatggtc agttgtgcca acatagtcgc tttcctgtat gaaaaaccat
     2221 tactgttatt gtgtacagta taaactggtt ttatatacag taaagaggct ggcgtaaaat
     2281 tagaataatt tttttgacca gccgagcttg gtgcttaatg tgttgaaata actgtaatct
     2341 ttcggatgaa tcagattcag atggtaatca cagcgacgaa tcaggacatc ttcaccttcc
     2401 tgaatcggca gtgctatctg gctgtcgcaa ctgatttcca ggtcgttacg gcgatgcgaa
     2461 aaacgcagac ggatcgtgct gctgctgttt atgaccagtg gtcgtgctga caacgtatgc
     2521 gggaacatgg gcaccagggt aatcgcatcc agagaggggg tcagaatagg accgcctgca
     2581 gagagggaat aggcggtgga gcctgttggc gtcgaaataa ttagtccatc agatcgctga
     2641 gaaaacgcaa agatctcgtc gatatacact tcgaactcaa tcatatgcgc cactttgcct
     2701 ggatgaagca ccacttcatt tatcgcggtg ctgatgcgtt tctggcaatc ttgctgacag
     2761 acttgcgctt ccagcaaaaa acgtttctcg ctgatgtagt ggccttccag cacatcggct
     2821 aactgttgct gggcgttatc ggggtcaagg tcagtcagga aacccaggtt gccacggttg
     2881 attccaataa ctttaatatc gtaacgggcg agtgtgcgtg ccgcgcccag catattaccg
     2941 tcgccaccaa cgactaccgc gagatcagct agttgcccaa tctccgcgag cgtgccagtt
     3001 ttcacattct tcagttgcag ttcgtgagcg atttgttgct caacgatgac ctcgtaacct
     3061 tttgtgcaca gccagcggta gagcatttca tgtgttgtca gtgcagtggg gtgccgtggg
     3121 tgtcccacaa tgccaataca cttgaaatga ttattcattt ttccgaggtc cttgttgcga
     3181 agattgatga caatgtgagt gcttcccttg aaaccctgaa actgatcccc ataataagcg
     3241 aagttagcga gatgaatgcg aaaaaaacgc ggagaaattc atgagtagta aagaacagaa
     3301 aacgcctgag gggcaagccc cggaagaaat tatcatggat cagcacgaag agattgaggc
     3361 agttgagcca gaagcttctg ctgagcaggt ggatccgcgc gatgaaaaaa ttgcgaatct
     3421 cgaagctcag ctggctgaag cccagacccg tgaacgtgac ggcattttgc gtgtaaaagc
     3481 cgaaatggaa aacctgcgtc gacgtactga actggatatt gaaaaagccc acaaattcgc
     3541 gctggagaaa ttcatcaacg aattgctgcc ggtgattgat agcctggatc gtgcgctgga
     3601 agtggctgat aaagctaacc cggatatgtc tgcgatggtt gaaggcattg agctgacgct
     3661 gaagtcgatg ctggatgttg tgcgtaagtt tggtgttgaa gtgatcgccg aaactaacgt
     3721 gccgctggac ccgaatgtgc atcaggctat cgcaatggtg gaatctgatg acgttgcgcc
     3781 aggtaacgta ctgggcatta tgcagaaggg ttatacgctg aatggtcgta cgattcgtgc
     3841 ggcgatggta actgtagcga aagcaaaagc gtaatttctg ttttcgtaat aattcacggc
     3901 cctgcatgcg aatgcagggc cgttttcgtt actccgccac actctcgcgc agcggtttca
     3961 caggaaaaac ttttacctgc ttaatcatat tgtcctgtac gtcgagaata tctatatcgt
     4021 actcgccaat acgcacgcgg gtgcctgcga cagggatctc ttccagtgcc tcaagaatga
     4081 cgccattaac cgtgcgggca tcatcttccg gtagatgcca gttaaaggct ttgttgattt
     4141 cccgcacgtt ggcggtgcca tcgataatca ccgaaccgtc gttttgcggc gtgacctctt
     4201 cggcaagtgt tggcgacatc gacgtagtga aatcgccgac aatctcttcc agaatatctt
     4261 caaccgtcac cagcccctga atgtctccat actcgttgac gaccaggccg actttctttt
     4321 tgttgcgctg aaactttacc aactgcgtgc tgagcggcgt accttccggc acaaaataga
     4381 tctcgtccgc ggcgcgcagc atggtttctt tggtgaactc ttttttctcc gacatcaacc
     4441 gccaggcttc acgtactcgc agcatactga tggcgtcgtc cagcgaatca cggtagagca
     4501 cgatgcgccc gtgaggtgag tgggagagtt ggcgcagaat cgatttccag tcatcgttga
     4561 tatcaatacc gataatttca ctgcgcggca ccatgatgtc atcaacggtc attttttcca
     4621 gatcgagcac cgacagcagc atatcctgat tgcgacggga aatttgtgag cgtgattcgt
     4681 gcacgatagt gcgcaactct tctttgctca aagagccgct aaccacgata tcggttttga
     4741 tacccatcat gcgcatcagc atacgggtga tagcattcag caaccagacc agcggcatca
     4801 tcaaaatttg cagcggagcc agcagaaaac tactcggata agcgactttt tccgggtaca
     4861 gcgcggcaat ggttttcggc aatacctcag caaataccag tacgacaaaa gtcagcacac
     4921 cagtcgcaat tgccacgccc gcatcgccgt acaaacgcat cccaacaata gtgccgagcg
     4981 cggaggcaag aatattgacc aggttattgc cgattaacac caggcttatc aggcggtctg
     5041 gcttacgcag caatttttcg acgcgtttgg ccgagcgatt accctgtttc gccatatgtc
     5101 gcagacgata gcggttgagg gtcatcattc cggtttcgga cccggaaaaa taggctgaaa
     5161 tgaccaccat gatgatcaga ataatgatca acgtagtagt agaaatgtgt tccaggggaa
     5221 actccttttc tgggtttagc tgattaactg ctggacaatt cggctgccga agtaggccag
     5281 tgtcagaatt accgcgcccg caacgttaaa ccagacgacg cggcgtccac gccatccttc
     5341 atggtaatgt ccccacagca gcacaatata gacaaaccac gccacgatag agagcacagc
     5401 cttgtcgata ttttccatgc taaataggtt gtgcatgtag aacaggccag tgcaaagcgt
     5461 gagcgttagc agcaccacgc caatctgcgt gatgtggaac attttacgct cgatactcat
     5521 caatggcggc atttcctggt taaatgccag cttcttgttc ttcagttggt aatcaatcca
     5581 cgccagttgc agcgcgtaca gggcggcgat aattagcgtg gcataggaaa agagcgataa
     5641 gccaatgtgc accagcatcc caggcgttgc ttccagatgg gtgatgtatt cattgggcat
     5701 gaaggtcgcc agcgccaggt tgataagcgc aaaggcatag acaatgggta gcagcaacca
     5761 gccacgattg cgagaagcca caatggtcat taccgtacag atcatcaaac tgaccaatga
     5821 accaacgttc agcaggctga ggttttgtcc gctatcaccg tcgggcagga tgcgggcttc
     5881 cagagcgatt gcgtggcaga ccagcgcaat gaccgcagaa ataatagcca tgcgccgcca
     5941 gccgccgttt ttttgcagca gaccgggaac aatcagcgca agactgacgg agtaggcgac
     6001 aagcgcgagc agagcaaaaa cgggcatagt gatgtcgaca gtttgagcaa taagaaagag
     6061 aagcagtata gcgtcaggtg aacgctgctc caaccgttgc ataacaacaa agacgccttc
     6121 atgttatact gcggcaaaat actgatgatg tgtcgcgatt gcggccaacc gtttccaccc
     6181 caggcgagag acaatgtttg ataatttaac cgatcgtttg tcgcgcacgc tgcgcaatat
     6241 cagtggccgt ggacgcctca ctgaagacaa cgtaaaagat acgctgcgcg aagtgcgcat
     6301 ggcgctgctg gaggcggatg tcgctctgcc ggtagtgcgt gagtttatca atcgcgtaaa
     6361 agagaaagcg gttggtcatg aagttaataa gagcctgacg ccggggcagg agttcgtcaa
     6421 aatcgtccgt aacgaactgg ttgcggcgat gggcgaagag aaccagaccc tgaacctggc
     6481 ggcgcaaccg cctgcggtcg tgctgatggc gggcctgcaa ggtgccggta aaacaaccag
     6541 cgttggtaaa ctcggtaagt tcctgcgcga gaagcacaag aagaaagtgc tggtggtttc
     6601 tgccgacgtt tatcgcccgg cggcaatcaa acagcttgag acgctggcag agcaggtggg
     6661 cgttgatttc ttcccttctg atgttggtca gaagccggta gatatcgtta acgcggcgct
     6721 gaaagaagcc aaactgaaat tctacgacgt gctgctggtg gataccgctg gtcgtctgca
     6781 cgttgacgaa gcgatgatgg acgagatcaa acaagtccat gcgtcgatta acccggttga
     6841 aaccctgttt gtggttgacg ccatgaccgg tcaggatgcg gccaatacgg caaaagcatt
     6901 caatgaagcg ttaccgctta ccggcgtagt gttgaccaaa gtggacggcg atgcccgcgg
     6961 cggtgcggcg ctctctattc gtcacatcac tggcaaaccg atcaagtttc tcggtgttgg
     7021 cgagaaaact gaggcgctgg agccgttcca tccggaccgc atcgcgtcgc gtattctcgg
     7081 catgggcgac gtactgtcgc tgatcgaaga tatcgaaagc aaagttgacc gcgcgcaggc
     7141 agagaaatta gccagcaagc tgaaaaaagg cgacggcttc gatctcaacg attttcttga
     7201 gcagctgcgc cagatgaaaa atatgggcgg catggctagc ctgatgggca agctgccggg
     7261 catggggcag atcccggata atgttaagtc gcagatggac gataaagtgc tggtgcgtat
     7321 ggaagccatc atcaactcga tgacgatgaa agagcgcgct aagccagaaa tcatcaaagg
     7381 ttcgcgtaag cgtcgtatcg ctgccggttg cggtatgcag gtgcaggacg ttaaccgcct
     7441 tctgaagcag ttcgacgaca tgcagcgcat gatgaagaaa atgaagaagg gcggaatggc
     7501 gaagatgatg agaagcatga agggtatgat gcccccaggc ttccctggtc gctaatcggc
     7561 ctggcttgtt tgccattttg gcaccgggat gtgctcgtca tcctcacgta ctgcgtgtac
     7621 gctccggtga ctgcgcgcac tccggcacca aactggctgc accgccgacg gccttttaca
     7681 gcagagttag caactgttga ttgcaattcc cgcaaaaatg agtaaaattt tcgggctttt
     7741 aatatgacac cggactccgt tcctcgatgg ggtccggttg ttttattcac acaagaggat
     7801 gttatggtaa ctattcgttt agcacgtcac ggcgctaaaa agcgtccgtt ctaccaggtt
     7861 gttgtcgctg acagccgtaa tgcacgcaac ggtcgcttca tcgagcgcgt tggtttcttc
     7921 aacccaatcg ctagcgaaaa agaagaaggc actcgcctgg atctggatcg catcgctcac
     7981 tgggttggcc agggcgcaac tatttctgat cgcgttgctg cgctgatcaa agaagtaaac
     8041 aaagcagctt aatctgtcac ggtggtcatg atgagcaaac aactcaccgc gcaagcacct
     8101 gttgatccca tcgttttggg aaaaatgggt tcgtcttacg gtattcgtgg gtggctcaga
     8161 gtgttttctt ccaccgaaga cgccgaaagc atttttgact atcagccctg gtttatccag
     8221 aaggcgggtc agtggcagca agtccagctg gaaagctgga agcaccacaa tcaggacatg
     8281 atcatcaagc tgaaaggcgt tgacgatcgt gatgcggcga acctgctgac gaattgtgaa
     8341 attgtcgtgg attcatcgca gctgcctcag cttgaagagg gggactacta ctggaaagac
     8401 ctgatgggct gccaggtagt aaccactgaa ggctacgatc tcggtaaagt cgtcgatatg
     8461 atggaaaccg gatctaatga cgttctcgtc attaaggcaa acctgaaaga tgcgtttggt
     8521 atcaaggaac gtctcgtacc gttcctcgat gggcaggtta tcaagaaagt cgatctcact
     8581 actcgttcaa tcgaagtaga ttgggatcct ggtttttaaa ccaccggata aacggtaaaa
     8641 gacggcgcta tgtggattgg cataattagc ctgtttcctg aaatgttccg cgcaattacc
     8701 gattacgggg taactggccg ggcagttaaa aatggcctgc tgagcatcca gagttggagt
     8761 cctcgcgact tcacgcatga ccggcaccgt accgtggacg atcgtcctta cggtggcgga
     8821 ccggggatgt taatgatggt gcaacccttg cgggacgcca ttcatgcagc aaaagccgcg
     8881 gcgggtgaag gcgcaaaggt gatttatctg tcaccacagg gacgcaagct tgatcaagcg
     8941 ggcgtcagcg aactggcaac gaatcaaaaa ttgattctgg tgtgcggtcg ctacgaaggt
     9001 atagatgagc gcgtgatcca aaccgaaatt gacgaagaat ggtcaatcgg cgattacgtt
     9061 ctcagtggtg gtgagttacc agcaatgacg ctgattgact ccgtttcccg gtttattccg
     9121 ggagtactgg gacatgaagc ctcggcaacg gaagattcct ttgctgaagg attgctggat
     9181 tgcccgcact atacgcggcc tgaggtgtta gaagggatgg aagttccgcc agtgttactg
     9241 tcgggcaacc atgccgagat acgtcgctgg cgtttgaaac agtcgctggg ccgtacctgg
     9301 cttagaagac ctgaacttct ggaaaacctg gctctgactg aagagcaagc aaggttgctg
     9361 gcggagttca aaacggaaca cgcacaacag caacataaac atgatgggat ggcgtaagcc
     9421 cccgagatat cagtttaccc aggataagag attaaattat gagcaatatt attaagcaac
     9481 ttgaacaaga gcagatgaag caggacgtac cttccttccg tccgggtgat accgtggaag
     9541 tgaaagtatg ggttgttgaa ggttccaaaa aacgtctgca ggcattcgag ggcgtggtta
     9601 tcgctattcg taaccgcggt ctgcactctg cattcactgt tcgtaaaatt tccaacggcg
     9661 aaggcgttga gcgtgtcttc cagactcact ctccggtagt tgacagcatt tctgtcaaac
     9721 gtcgtggtgc tgttcgtaaa gctaaactgt actacctgcg tgagcgtact ggtaaggctg
     9781 ctcgtatcaa agagcgtctt aactaagatt tcgcttaagc gacatcctgt taagaagggc
     9841 tggccaattg gctggccctt tttttgtctg tttgctcgtg ttttaagggg ttgtaatcac
     9901 cactgcgacg cggcggttct cggcgcggcc ttgggcggtc ttgttactgg ctatgggata
     9961 tttttttcct aaaccctgtg tggtgagatt gctgcgtgga atttgtccac caatagccca
    10021 tgcatcggcc acgacattcg cccgtttcaa tgataagcct tcgttgtaac tgtcttcacc
    10081 atagttatcg gtgtgtccat ccatacgggc atgtgttagc cctgtcgagg ccaatttggc
    10141 tgccatggtt tggatctgtt gctggctttc cgggagcaat ttgtagtcat tttttgcgaa
    10201 cagaatggca tctgataagc ccagcgacca gtcgccggcg gattcagtaa atccataaga
    10261 ttgcatagcg gcgacttgct caggagtaaa ctttccctga ggggactggc agccagtcag
    10321 tattagtgag gtgaaaatca ggggtgctac taggtgcttt atcatatcga tatatccttg
    10381 ttatctcacc agcttttcgg cacgctggtg tttggcctga tacatattgt gatcggcaag
    10441 ctcttgtaat ttttccgcag aggcgtgctc aatggtcatc gcgtaaccaa tgcttaatgt
    10501 catggtggtc tgatggccat tatgaagatc aaacgggaga ttaaagattt gtgtcagtgc
    10561 tgagcatatc tgctgcactt cagattctga ctgtacatca tagagcacca tagcgaattc
    10621 atcgccgccc aggcggtatg ctttatgtcg cagcccgcca acttcagcta accgttttgc
    10681 gatttcaatc aagactctat cgcccgtcgc atgaccccag gtatcattga tgtatttgaa
    10741 attatcgcca tcaagaaata gtaacgccga cgtttttcgg gcatcggaat tgttcatcaa
    10801 cgtgttgatg ccgctacgaa acgctgcgcg gttagccagc ccggttaatg ggtcatgtag
    10861 cgcggtacgt agaagctgcg catttttagc ctgtaaacga agctgccact cttccatttc
    10921 atccagcaga ctgttgaagt cgagagcgaa gcggtgaaac tcagcgatac gttcttccga
    10981 aactcgtcgg gaaaaattgc ggttggaacg cacatcatgt acgacatcgg tgatattttt
    11041 cagtgcttcc accaggccat tgtgcaaatg gcgggtgagg gtaattgcga tgcctgatgc
    11101 cagcagaata caaccggtca gtacggcgag cgaaaaccag ataaaatggc tgattgaact
    11161 gtcgcgagcg gttaagcgta cttcgccaat ggtttcacca ttgtgacgaa tcggctgaat
    11221 gatgggggcg gggaagagcc agtggcttat gaaattgctg aaagtatcgc ctggatcctt
    11281 acgggtgtaa tgccaggacg ccagaatatt ttgctgctta tcacgtactt ctgcagttga
    11341 aaattgccct tgctggccca gcgctgccag tgtttcagtt gctgcagggc catcggcaaa
    11401 aacgacagct gcttccagac tgtaagt
//
LOCUS       DAJTXQ010000123        11357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000123 DAJTXQ010000000
VERSION     DAJTXQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.123"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="OO515_003260"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="OO515_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6577115.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="OO515_003261"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="OO515_003261"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6577116.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="OO515_003262"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="OO515_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6577117.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="OO515_003263"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="OO515_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6577118.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="OO515_003264"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="OO515_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6577119.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="OO515_003265"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="OO515_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6577120.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="OO515_003266"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="OO515_003266"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6577121.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="OO515_003267"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="OO515_003267"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6577122.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="OO515_003268"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="OO515_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6577123.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="OO515_003269"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="OO515_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6577124.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="OO515_003270"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="OO515_003270"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6577125.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="OO515_003271"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="OO515_003271"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6577126.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11357
                     /locus_tag="OO515_003272"
     CDS             10898..>11357
                     /locus_tag="OO515_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6577127.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2874 a         2631 c         3056 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacgaa
//
LOCUS       DAJTXQ010000124        11338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000124 DAJTXQ010000000
VERSION     DAJTXQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.124"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..236
                     /locus_tag="OO515_003273"
                     /pseudo
     CDS             24..236
                     /locus_tag="OO515_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            328..3012
                     /locus_tag="OO515_003274"
     CDS             328..3012
                     /locus_tag="OO515_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6577128.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3009..4097
                     /locus_tag="OO515_003275"
     CDS             3009..4097
                     /locus_tag="OO515_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6577129.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4180..5477)
                     /gene="exuT"
                     /locus_tag="OO515_003276"
                     /pseudo
     CDS             complement(4180..5477)
                     /gene="exuT"
                     /locus_tag="OO515_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5960..7372
                     /gene="uxaC"
                     /locus_tag="OO515_003277"
     CDS             5960..7372
                     /gene="uxaC"
                     /locus_tag="OO515_003277"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6577130.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7387..8874
                     /gene="uxaA"
                     /locus_tag="OO515_003278"
     CDS             7387..8874
                     /gene="uxaA"
                     /locus_tag="OO515_003278"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6577131.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8957..9508
                     /locus_tag="OO515_003279"
     CDS             8957..9508
                     /locus_tag="OO515_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6577132.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9513..10757)
                     /gene="sstT"
                     /locus_tag="OO515_003280"
     CDS             complement(9513..10757)
                     /gene="sstT"
                     /locus_tag="OO515_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6577133.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2978 a         2778 c         2942 g         2640 t
ORIGIN      
        1 agcggtatta agcgcagcta tttagcaaga ttgttccgct gacagtaacc tggggcggtg
       61 aagaaattaa agctgatgcg gcaaccactt ttaccgcaac caaaatcttt gcttccgatg
      121 cgttaactaa cggttctctg gctaaaaacc tgatgtttgc tcaaaccacc aaaggtgttc
      181 tggagacagg catctaccgt ggtgtagtaa gcatttatct gtctcaggat atctaatcca
      241 gataacagaa ttaacacgta acaaagggag gggtagccct cccttatttt aactgtttta
      301 gtatttgtta tggatttcag gattgccatg gataaaaaat tactggctct tttgatcctg
      361 gcgagtctca gcccggcaga ggcgacatta accgaaattc ccgcagggtt tgaggttatt
      421 gctcagggac agcaggagta tatcgaggtt tatttttcag ggaaaagtct cggtaaatat
      481 tatgcaatgg ttaatcttga taccgtaaca tttcttgatc cagcaagtct atataacaag
      541 ctggaactgg atgtagacga tcagaaaatc gcgcatatag tgaaagaaaa attatcgcag
      601 ccgctagctc accacggtga attggcttgc ggttatgtac gtactgactc agggtgtggt
      661 tttctgaata ccgatacgct ggaaataatc tataatgatg aagaaagttc ggcaacgttg
      721 tttattaatc cgcaatggaa ttcagctttc gatgcgaagt cattatattt aaatccagac
      781 aaaaatactg ttaatgcttt tatacatcag caagacatca atgttctggc acaggatgat
      841 taccaatcgt tgtctattca gggaaacggt gcgctgggaa taacagaaaa tagttatatt
      901 ggtgcacact ggaatttcaa cggttatgat gcagatgatg tcagtgacag taatgctgac
      961 gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt attatgtgca ggcgggtcgc
     1021 atggacaacc gcacactatt taatgcacaa ggcgggaact ttacctttaa ctttctgcca
     1081 ctcggtgcaa ttgatgggat gcgtatcggg tcgacactga gctatttaaa ccaggcgcaa
     1141 agccagcaag gaaccccagt aatggttctg ctttcgcgca attctcgtgt tgacgcttat
     1201 cgtaatgagc aacttttggg atcgttttat ctcaatagtg gttcgcaatt tattgatacc
     1261 agttcctttc cgccaggcag ctacagcgtg gcattaaaag tctacgaaaa taaccaactc
     1321 acccgcaccg agcttgtgcc gtttaccaaa accggcggtc tgactgacgg aaatgcgcaa
     1381 tggttcttac aggcaggtaa aactacatca caggtttctg atgatgaaag ttcagcttat
     1441 cagctaggag tacgcctgcc attacatccg caatatgagc tctacgcagg gctggcgaat
     1501 gccgatgatg tgagtgcttt cgagttaggt aataactgga cggcagattt aggcggggtg
     1561 gggaatcttg caatcagcgc cagcgtgttc cgtaacgatg acggcggcaa aggtgatatg
     1621 caacaggcca actggagtaa tccgggatgg ccgacgttgg gcttttatcg gaccaactct
     1681 gacggcgatg cttgtacaac cgacagcaga gagagctata acgccttaag ctgttatgaa
     1741 agtatttccg cgacggtttc acagaatttt gtcggctgga atatgatgct gggttatacc
     1801 cgcacacaaa ataacactga tgatagtttg cgttgggata aacagcagag ctttgaaaat
     1861 aactatcttc gccagacaac agcgcaaagt atctctgaaa ctgtacaact tagcgcttcc
     1921 cgcgcttttg tgatgcgtga ctggattttg agtacgtcag tcggtgtttt ccatcgtaat
     1981 gacaacggtg gcgataacga cgacaacggc ttgtacttat cgttttcgtt atctgacacg
     2041 ccaacgatgg atagcaataa caacagccat tcaacaaatg tgtctacgga ttatcgttat
     2101 agcgaacagg atggcgatca aacgtcatgg cagttatcgc atacctttta taacgattca
     2161 ttcagccata aagaacttgg cgtaaccgtc ggaggcctga acaccgatac cataaacagc
     2221 gcggttaacg ggcgttggga tgggcaatac ggaaatgtct acgctaccgt atctgatagt
     2281 tatgaccgta agaatcatga tcatctttcg gcctttacgg gcacttatag ctctacgctg
     2341 gctgtcagtt gctatggcgt taatttggga gccagtggta cagacgattt gctgggtgcg
     2401 gtactggtgg atgtgaaagg cttctctgaa caggatgaag agagtcagga tctgcaactc
     2461 gaagcgcggg tggcaggcag ccgaactttg cagcttggtc aaagtgacag tgtgttgttc
     2521 ccttatcctg gatttcagtc tggttttgtt gaggttaacg acagtagcca gggcaatcag
     2581 caggggacaa caaacatcat taacggtgca gggaatcgtg aattaatgtt gttgcctggc
     2641 aagctgcgct atcgcgaagt gtctgccagc tttaactaca actatatcgg tcgtttatta
     2701 ctgccggcag cggtgaaaaa attcccgatt gtggggctga acagcgccat gttactggta
     2761 gctgaagatg gaggatttac acttgaaatt aacggcagcg aaaaagagct ctatctgctt
     2821 tccgggcagc aattccttaa gtgtccactg agtgttgtaa agaaacgcgc cagcattcgt
     2881 tacagcggag atgttacttg tagtgtggtg acttattcac aattaccgga gtcgattcag
     2941 gttcaggcac agttgaaaca gcctaaatta cgtggaaacg ttcagacggc gcaaagggag
     3001 gttgcaccat gagaaatcga ttgattgcgg cgatattggg cttgtttggc acgctcactg
     3061 gcgttcaggc agctcctgac gtgaccagtg aaattacgta tgatttggca tctggtagag
     3121 cggattatta cttctggaag gatgaagcgt cggcaggaaa taatggatat atgtggtatg
     3181 aatgttcgta tcctgacctc caacaaacct gtacagctaa tggaaatata tcgacagtac
     3241 aaatctattt aactgaacaa cgcagtggga tgcgttggcc ggtaaaactc aaaggattta
     3301 aaacagccat tgtaagtagc gatgaagcgc cgccaggatg caaggggggc aaagggcttc
     3361 agacgaatct taaggattct aatagatctt catgtacaga agatggtcaa cattattata
     3421 tatacgatac aaagtttctt acgctctacc ttgagcagac agagatgaag aatttgccga
     3481 ttggtggcgt ctggaagggg aaagttaaat tacattcgaa cagcccggcc caggactatt
     3541 tcgcaaatat taccctgaat acgctcgacc ccaaccatat tgacgtgttc ttcccggagt
     3601 tcgcccacgc cacgccaagg gtgcagttag acttgcatcc aacaggaagc gttaacggca
     3661 gcaactacgc gcaagatctg accatgttgg acatgtgcct gtacgatggt tttaacggta
     3721 atgccatcag ttatgaaatc atgctcaaag atgaagggcg accagctgca gggcgcagag
     3781 acggtgactt ctctatctat cgtcaaggag ggaccaccac cgacgaggga gaacgcattg
     3841 attaccgggt caaaatgtac aacccggaaa ccggtgggca aattgatgtg cgcaataatg
     3901 aaaatatggt ctggaacagc attaacctga aacgtgtgcg tccggtggta ctgcccggta
     3961 ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct ggcagtcgat aaattcagcg
     4021 tgatggataa acaggccggg tactacatgg gcaaattgtc agtaatcttt acgccttcct
     4081 tgccaaccat caattgaccc cgcagaccag aaaaaactaa acaacgaagc cgcctgataa
     4141 ggcggcttcg tcattacaac ttacgttaaa cgttaaacgt taatgttgcg gtgcgggatc
     4201 gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc tgcaatacgg tccagataac
     4261 cagcgcaccc agcaggtcaa atactgccag aactgcgaac agcgggctga agccgatggt
     4321 gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc
     4381 ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat
     4441 cagcgcgcca gacagtgcct ggtgggcaaa accaccgata cacagcagca taattgcgac
     4501 atacgggttg gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt
     4561 tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg
     4621 caggtaacca ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat
     4681 cgcgatctct ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt
     4741 aaaagtaccc caggccggtt ctgccaggaa acgcggcagt gcgatacccc agaactgacg
     4801 gttacgcagg atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc
     4861 ctgaccatta ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg
     4921 tttatagaaa atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa
     4981 tgccatctgc cagctgtgca tcacgattgc cataccacca gcggcggcgc aatcatcgca
     5041 ccaatcgaag aacctacgtt aaagtagcct actgcgatgg aacgctcttt cgccgggaac
     5101 cattcggagc tggctttcag acccgccggg atcatcgctg cttccgcggc accgaccgca
     5161 ccacgagcaa cagccaggcc accccagcta cctgccagcg cggttgcacc acagaacacg
     5221 gcccacagca cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacataa
     5281 cctgctaccg gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac
     5341 tgttgggtgg agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta
     5401 cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac
     5461 cctttaattt tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca
     5521 tcatcccgcc agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac
     5581 gccccgaacg ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat
     5641 tcatctaaca aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc
     5701 agaaagataa cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt
     5761 tgtagggaca ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta
     5821 tcactttgag agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg
     5881 gactctggaa attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact
     5941 cgttgagagg aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga
     6001 atttgcccgc cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg
     6061 ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg
     6121 gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct
     6181 gtgtaccggt gatgcatctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca
     6241 cactatcggc aacccgttat accactggac gcaccttgaa ctgcgtcgtc cgtttggtat
     6301 cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact
     6361 gctggcgcag gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt
     6421 cggcaccacc gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg
     6481 ctctttcacc atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca
     6541 ggcgaccttt aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg
     6601 ctttgctgac ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg
     6661 taaagtgtct gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact
     6721 ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca
     6781 gttcaaaact gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca
     6841 gcagtaccac attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc
     6901 ggatgtaggc tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct
     6961 gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg
     7021 cgataacgaa gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa
     7081 aatgcagttc ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat
     7141 gacccaactg gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg
     7201 tagcttcctg tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg
     7261 tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa
     7321 aaatatttgc tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg
     7381 gttgatatgc aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat
     7441 ttggctgaag gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt
     7501 gctcgtggac ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat
     7561 ggcctgccga ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac
     7621 aatacgcgca cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat
     7681 ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc
     7741 ggggtgcgta acgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg
     7801 caaatccaga accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc
     7861 ctcttcagcc acacctacgg ctgctcacaa ctgggcgacg atcacatcaa catccgcacc
     7921 atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc
     7981 tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc
     8041 gttcatttca tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg
     8101 catcagctgt ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg
     8161 aagtttggtc tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg
     8221 ctggggcgtt tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg
     8281 ccggagatgt ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt
     8341 gaaaaactgg tcaccatggt caacgacttt aaacagtact ttattgccca tgatcagccg
     8401 atctacgaaa acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa
     8461 tcacttggct gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc
     8521 gagcgtctga aaacgcctgg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg
     8581 accagcgccc tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg
     8641 ccatacggtg gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag
     8701 aaaaaacact ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag
     8761 ttgctggaag aatttatcga caccatcgtt gagtttgcca acggtaagca aacttgcaac
     8821 gagcgtaacg acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga
     8881 aatgagttgt taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac
     8941 ctgataagga tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt
     9001 tctgattggt atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc
     9061 ggtctatagc gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc
     9121 cagtgccatc cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt
     9181 aatggcgatt tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt
     9241 cgaactattg ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg
     9301 gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg
     9361 ggcggggtcg ataggcggca caatgattga gggtagtttt ctgcttatga atggcttgaa
     9421 tatcattcgt ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac
     9481 ccctcccgcc gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga
     9541 cgatcgtctt ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc
     9601 gcggtttcgc aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg
     9661 gcgatatcgt tcgaaatacc gaacatatta caggccagcg ggatcagcag cagagaaccc
     9721 cccgccacgc cggatgcgcc acaggcacac agagaagcca ccacgctcaa cagcagcgcc
     9781 gtgggcagat cgaccgggat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg
     9841 gtgattgctg cgcccgccat attgatggtg gcacccagcg gaatagaaac ggaataggta
     9901 tcgcgatcca gattcagctt ttcacacagt gccatattca ccggaatgtt cgccgcagag
     9961 ctgcgggtga agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac
    10021 gggttacgac gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac
    10081 atacagccaa ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg
    10141 gttgccaggg tagaagaaac cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt
    10201 ttcaccataa aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta
    10261 ccgtgacgca gtgcgaaacc gaggccaatt gcccacacca ggatcccgat gtagttacct
    10321 ttcagcagcg cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttct
    10381 acaatgcctg acggtggcga aatatcaccc gcgctactgg ataagtgcag ggtagaaggg
    10441 aaggcgaagc tgaaaacgac tgcggccaga gcagcagaga aggtacccag cagatagagg
    10501 aacaaaatag ggcggatatt ggttttctgc ccatgctggt ggttagcaat agatgccatc
    10561 accagcatca acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct
    10621 aacagaccaa cagcttccgc cgcgggtttt gatatccatg ccagaagaat ccccagaaca
    10681 aggccgacca ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt
    10741 gaacgttgcg tagtcatttt tcgatccttt cattgtgtta tcgcacatcc ctggtgcgtt
    10801 ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt
    10861 acagcttcgg gaagcgaaaa atgctggatt ttaaagagaa gtcatatttt gaaacttaat
    10921 ggttacattt gtttgacata aacagaggga aggactggct actaaggctg attagataac
    10981 atatgggcaa aactatttgg aatatgagaa cggttgcgct cctttaatgt gctatgccat
    11041 ataaagtcac tggcgcaggg agcgcgcagg gggcggccaa tcgccgccgc cccctgcacc
    11101 cccaggctct ggcgaacaaa atcgccgctg cgcgattccc tcagcttatc ccttgcggct
    11161 accgggtcgg gcgcaaggga acgtccctgt aagatgcgcc ctcgccgcgc atccatgcgc
    11221 gtcgccccgg cctccaggga acgcttcggc gattttgacg ccaccaacac ccgtgcggtc
    11281 tttttgtaaa gagctacaag ccgattacat aattaatagc tcacctttgt ctggaatg
//
LOCUS       DAJTXQ010000125        11242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000125 DAJTXQ010000000
VERSION     DAJTXQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.125"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..308
                     /locus_tag="OO515_003281"
     CDS             <1..308
                     /locus_tag="OO515_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6577134.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="OO515_003282"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="OO515_003282"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6577135.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OO515_003283"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OO515_003283"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6577136.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="OO515_003284"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="OO515_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6577137.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="OO515_003285"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="OO515_003285"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6577138.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="OO515_003286"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="OO515_003286"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6577139.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="OO515_003287"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="OO515_003287"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6577140.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="OO515_003288"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="OO515_003288"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6577141.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /locus_tag="OO515_003289"
     CDS             complement(8116..9762)
                     /locus_tag="OO515_003289"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6577142.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /locus_tag="OO515_003290"
     CDS             complement(9765..9815)
                     /locus_tag="OO515_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577143.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="OO515_003291"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="OO515_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6577144.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="OO515_003292"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="OO515_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAJTXQ010000126        11171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000126 DAJTXQ010000000
VERSION     DAJTXQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.126"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            296..1678
                     /gene="dosC"
                     /locus_tag="OO515_003293"
     CDS             296..1678
                     /gene="dosC"
                     /locus_tag="OO515_003293"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6577145.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1703..4102
                     /gene="dosP"
                     /locus_tag="OO515_003294"
                     /gene_synonym="pdeO"
     CDS             1703..4102
                     /gene="dosP"
                     /locus_tag="OO515_003294"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6577146.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4361..4942
                     /gene="ddpX"
                     /locus_tag="OO515_003295"
     CDS             4361..4942
                     /gene="ddpX"
                     /locus_tag="OO515_003295"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6577147.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4956..6506
                     /locus_tag="OO515_003296"
     CDS             4956..6506
                     /locus_tag="OO515_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6577148.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6508..7530
                     /locus_tag="OO515_003297"
     CDS             6508..7530
                     /locus_tag="OO515_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6577149.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7527..8423
                     /locus_tag="OO515_003298"
     CDS             7527..8423
                     /locus_tag="OO515_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6577150.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8420..9406
                     /locus_tag="OO515_003299"
     CDS             8420..9406
                     /locus_tag="OO515_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6577151.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            9435..10325
                     /locus_tag="OO515_003300"
     CDS             9435..10325
                     /locus_tag="OO515_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6577152.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(10381..10812)
                     /gene="osmC"
                     /locus_tag="OO515_003301"
     CDS             complement(10381..10812)
                     /gene="osmC"
                     /locus_tag="OO515_003301"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6577153.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2792 a         2756 c         2834 g         2789 t
ORIGIN      
        1 atcatgctta cataagtcaa attaaccacg caattaacca cacttagtta aacagctata
       61 aatccctaat aaaaatgtat gaatatgaaa aaccatacga tattcgctgt caccgaaaaa
      121 taattacgat gggctatact cttctgaaga acagtagcgt agattgaaac tgtcctaagt
      181 cgcttatttc ttttcagtat atcttcatat ttcaggagaa tatggtgcag ttagtcaatc
      241 tcgcacgttg cgttttgatt ccgtctgatt tcccagcctt ataagggtgt gaattatgga
      301 gatgtatttt aaaagaatga aagatgagtg gaccggactt gtcgaacagg cagatccact
      361 cattcgtgct aaagccgcgg aaattgccgt tgcgcatgct cattatctga gtatcgagtt
      421 ttatcgaatt gtccgcatcg acccgcatgc cgaagaattc ttgagtaatg aacaagttga
      481 gcggcagttg aaaagtgcga tggaacgctg gattattaac gtgctttctg cccaggttga
      541 cgatgtcgaa aggctaatac aaatccagca taccgtcgcg gaagtgcatg cccgcatagg
      601 aattccggta gaaattgtcg agatggggtt tcgggtgctg aaaaaaatcc tctatccggt
      661 aatcttctct tcggattatt ccgccgcaga aaaacttcag gtctatcatt tctcgattaa
      721 cagtattgat atcgcgatgg aagtgatgac ccgcgcgttt acctttagtg acagtagtgc
      781 ctcaaaggaa gatgaaaatt atcgtatctt ctcgttactg gaaaacgccg aagaagaaaa
      841 agaacggcaa atagcctcaa tactttcatg ggaaatagat attatctata aaatcctgct
      901 ggattctgat ttaggcagta gtttgccatt aagccaggct gattttggcc tgtggtttaa
      961 ccataaaggt cgacattatt ttagtggcat tgccgaagta ggccatatct cccgtctcat
     1021 tcaggatttc gacggtattt tcaatcaaac catgcgtaac accaggaatt tgaataacag
     1081 aagtctgcgg gtgaaatttt tattacagat aagaaatacc gtatcgcaga ttattacctt
     1141 gctgcgtgaa ttgtttgaag aagtatcgcg ccacgaagtc ggtatggatg tactaacgaa
     1201 attacttaac cgccgtttcc tgccgactat cttcaaacgc gaaattgccc atgccaaccg
     1261 gaccggtaca ccgctgtcag tgctgattat tgacgttgat aaattcaaag agatcaacga
     1321 tacgtggggc cataacactg gcgatgaaat tctgcgtaaa gtctctcagg ccttttatga
     1381 caacgtccac agtagtgatt atgtttttcg ctacgggggc gatgaattta tcattgtttt
     1441 gactgaagct tctgaaaacg aaacgttacg taccgcagaa cgtattcgca gtcgggtgga
     1501 gaaaaccaaa ctgaaagccg caaacggcga ggatattgcc ctctcacttt ccatcggtgc
     1561 cgccatgttt aatggtcatc ctgactatga gcgcctcatt caaatagccg atgaagctct
     1621 gtatatcgcc aaaagacgag gtagaaaccg tgttgaactc tggaaagcca gtctttagat
     1681 gcgccaggat gcagaggtaa tcatgaagct aaccgatgcg gaaaatgccg ccgatggcat
     1741 ttttttcccc gcccttgaac aaaatatgat gggcgcggtg ttaattaacg aaaatgatga
     1801 agtgatgttt ttcaaccccg ccgcagagaa gctctggggg tacaaacgtg aagaagtcat
     1861 tggcaataac attgatatgc tgattccgcg ggatttgcgt cctgcgcatc ctgaatacat
     1921 tcgtcataac cgtgaaggtg gtaaagcgcg cgtcgagggg atgagtcggg agctgcagct
     1981 ggagaaaaaa gacggcagta aaatctggac ccgttttgcg ctatcgaaag tgagcgccga
     2041 ggggaaagtt tattacctgg cgctggtacg ggatgccagc gtagaaatgg cgcaaaaaga
     2101 acagacccga caattgatta ttgccgttga ccatctcgac cgaccggtga ttgtcctcga
     2161 tccggaacgc catattgtgc agtgcaatcg cgcatttacc gaaatgtttg gttactgcat
     2221 tagcgaagcc agcggtatgc agcccgatac actcctgaac attcctgaat tccctgccga
     2281 taaccgcatt cgtttacaac agttgctatg gaaaaccgcc cgcgatcagg acgaatttct
     2341 gctgttgaca cgcaccggtg aaaaaatctg gattaaagcc tctatcagcc cggtttatga
     2401 cgtgctcgcg catctgcaga acctggtaat gaccttctcg gatatcaccg aagaacggca
     2461 gatccgccag cttgaaggca atattctcgc cgccatgtgc agcagcccgc catttcatga
     2521 aatgggggaa atcatttgtc gtaacatcga atctgttctc aacgaatcgc atgtttcgct
     2581 gttcgcactg cgcaacggga tgccgataca ctgggcgtca tcttcgcacg gtgcagaagt
     2641 tcaaaatgcg caaagctggt cagcgaccat tcgtcagcgt gatggtgcgc ctgcggggat
     2701 cctgcaaatt aaaacctcgt caggagcaga aaccagcgcc tttatcgaac gcgtggcaga
     2761 tatcagccag catatggccg cgctggcgct ggaacaggaa aaaagccgtc agcatattga
     2821 acaactcatc caatttgatc cgatgaccgg tctgccaaat cgcaataacc tgcacaatta
     2881 cctcgatgac cttgtcgaca aagccgtctc tccggtggtg tatctcatcg gtgttgacca
     2941 tattcaggat gtgattgata gccttggcta tgcgtgggcc gatcaggcat tgctggaagt
     3001 ggtcaatcgc tttcgtgaaa aactcaaacc ggatcagtat ctctgtcgta tcgaaggtac
     3061 gcagtttgtc ctcgtgagcc tggaaaacga cgtcagtaac attacccaaa tcgccgatga
     3121 gctacggaat gtggtcagca agccgataat gattgacgat aaaccattcc cgctaacctt
     3181 gagtattggc atcagctacg acgtgggtaa aaaccgcgat tacctgctct ccactgctca
     3241 caatgcaatg gattatattc gcaagaatgg cggtaacggc tggcagttct tcagcccggc
     3301 gatgaacgaa atggtaaaag agcgtttggt tttaggcgca gcgctgaaag aagcgattag
     3361 caataaccaa ctgaaactgg tttaccagcc gcaaatcttc gcagaaacgg gtgaactgta
     3421 cggcatcgaa gcccttgctc gctggcacga tccccagcat ggtcatgtac ccccttcacg
     3481 gtttattcct ctcgcagaag agattggtga aatcgaaaat attgggcgct gggtcatcgc
     3541 ggaagcttgc cgtcagttag cagaatggcg tagccagaat attcatatcc cggcgttatc
     3601 cgtgaacttg tcggcgctgc actttcgcag taatcagctg cctaatcagg tgtctgatgc
     3661 aatgcaagcc tggggtattg acggccacca gctgacggta gaaatcacgg aaagcatgat
     3721 gatggaacac gataccgaaa tctttaagcg cattcagatc ctgcgcgata tgggcgtggg
     3781 cttatcggta gatgattttg gcacgggctt ttccggatta tcccgcttag tcagtcttcc
     3841 ggtaacggaa atcaaaattg acaaaagttt tgtcgatcgt tgtctgacgg aaaaacgcat
     3901 ccttgcctta cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc
     3961 ggaaggcgtc gaaaccaaag agcaatttga gatgctacgc aagatccact gtcgcgttat
     4021 tcagggatat ttcttttccc gccccctacc cgccgaggaa attccaggct ggatgagcag
     4081 cgtgttaccg ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt tttcatttaa
     4141 cgtgacgctg tcacttaaat actgtgattt cagctacgat tccgggagat tccttcctta
     4201 acttcccctc ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa aagttaaaaa
     4261 atataacttt aagccagcat tctggcgctt atcccggcat ggcatgagat ctgcataagc
     4321 ggaaagcgca gcaatttttg tcttaataca ggaaccgttt atgtcggata ccaccgaact
     4381 ggttgattta gccgtgatct tccctgatct ggagatcgaa ttgaaatacg cctgcgctga
     4441 taacatcaca ggtaaagcta tttaccagca agcgcgttgt ctgttacaca aggatgcgat
     4501 taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca ggcttacaac tggtgattta
     4561 cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc cagacccgca
     4621 atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga tcgacctgac
     4681 gcttcgtgat gagcacggga acatcctgga tatgggcgca gggttcgatg aaatgcacga
     4741 gcgctcccat gcctatcacc cttccgtccc gcccgccgct cagcgcaatc gcctgttgct
     4801 gaatgcgata atgactggtg gcggttttgt cggtatctcc agcgaatggt ggcacttcga
     4861 attaccccag gcagcgagtt accctctgct cgctgatcaa ttctcttgtt ttatatcccc
     4921 tggcacacag cacgtcagtt aatcaggaaa ctgtcatgaa gagatcgata tcgtttcgtc
     4981 ccacattgct cgcgctcgtc cttgccacaa ctttcccggt tgcgcacgcc gccgtaccga
     5041 aagatatgct ggtgattggt aaggccgccg atccacaaac cctcgacccg gcggtaacaa
     5101 tagataataa cgactggaca gtgacctacc cgtcttatca gcggctggtt cagtacaaaa
     5161 cggacggtga taaaggctca accgacgttg aaggcgatct ggcaagtagc tggaaagcgt
     5221 ctgacgatca aaaagagtgg acgttcaccc tgaaggacaa tgctaaattt gccgatggca
     5281 cacctgtcac tgccgaagca gtaaaactct cttttgagcg gttactaaaa atcggccagg
     5341 ggccagcaga agcatttccc aaagatttaa agattgatac tcccgacgaa catacggtga
     5401 agtttaccct tagccaacca ttcgcaccgt tcctctacac gctagcgaat gacggtgcat
     5461 ccattatcaa tccggcggtc ttaaaggaac atgcagcgga tgatgctcgc ggcttcctcg
     5521 cgcaaaatac cgccggttcc ggaccattta tgctgaaaag cgggcaaaaa ggtcagcaat
     5581 tagttctggt gccaaatccg cattaccccg gcaataaacc gaactttaag cgagtatcgg
     5641 taaaaattat cggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc gacattgaca
     5701 ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaat aaagtcaatg
     5761 tggcagagta tccgtcactg cgcgttacct atctgtatct gaataacagc aaagcgcctc
     5821 ttaatcaggc ggatctgcgt cgggccattt cctggtctac cgattatcag ggcatggtta
     5881 acggcattct gagtggtaac ggaaaacaga tgcgcggccc gattccggaa ggcatgtggg
     5941 gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa gccgaatggg
     6001 ataaagtgac gagcaaaccc accagcctga cgtttctcta ctccgataac gatccgaact
     6061 gggagcctat tgctctggcg acacaatcca gtctcaacaa gctgggcatc aatgtgaagc
     6121 tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat tacgacattg
     6181 cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat tactggtttg
     6241 agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt gaggtcgata
     6301 agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccgg gactaccagc
     6361 aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag aaaaactacc
     6421 aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg gaacaggtct
     6481 tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg ccaacgctgc
     6541 tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat ctcgcatctg
     6601 atccctggcg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc tatcgtggaa
     6661 aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta ccgctacttc
     6721 agcgacctgt ttcatggtga cctgggaaca tccattcgta ccgggcgtcc ggtgctggaa
     6781 gagttgcgta tatttttccc ggcgacgctg gaactggctt tttgtgccct gctgctggca
     6841 ctcctgattg gcatcccgct gggcatactc tctgcagtct ggcgaaatcg ctggctggat
     6901 catctggtgc gaataatggc cattaccgga atctccacac ctgcgttctg gcttggactg
     6961 ggcgtcattg tgctgtttta tggtcatctg caaattcttc ccggcggcgg aaggcttgat
     7021 gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc gctgcttgaa
     7081 ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc attaacgctg
     7141 gcgttcgttc acctgggaat tgtcgctcgc cagatccgct cagcgatgct ggaacaattg
     7201 agtgaagact acattcgtac cgcccgggcc agcggcttgc ccggctggta tatcgtttta
     7261 tgttatgcgc tacccaatgc gttgatccca tcgattaccg tattgggtct ggcgctgggc
     7321 gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg aatgggtgca
     7381 tgggtagtaa catcaataca ggcgctcgac ttcccggcag tgatgggctt tgccgtcgtg
     7441 gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt gtggattgat
     7501 ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc gccgtacgcc
     7561 cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa ggcagtccgc
     7621 tcaccgtgac cggcgcagtc atcattgtat taatgctatt gatgatgatt ttttcaccgt
     7681 ggcttgcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg ccgccttctg
     7741 cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc gtactggtcg
     7801 gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt atgattggtt
     7861 cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc atcatgcgca
     7921 tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg gctgccgctc
     7981 tcgggccgag tttatttaac gccatgctgg cgattgctat tgtgcgaatt cccttttatg
     8041 tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt caggcggcga
     8101 aaacctttgg tgcgtcgcgt tggcatctga tcagctggca tattttacgt aactccctgc
     8161 cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg gccgccacgt
     8221 tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg atggtagcta
     8281 atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg gcagcgattt
     8341 tgcttaccgc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg cttgacccga
     8401 aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacagc tgcatttgag
     8461 tttccccggt tttaacggtt atgttcacgt gctcaacaat gtgtccttgc agattaaccg
     8521 cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca ccgcaatgct
     8581 gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga tttcactgct
     8641 gggagacgat gttcttaacg ctcgggagaa acagcttcgt cagtggcgcg gcgcacgagt
     8701 ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa taggtcttca
     8761 gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca gagctaaagc
     8821 gattaccctg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt cgcgctatcc
     8881 gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat tctcctgcga
     8941 gccgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg tacagttgca
     9001 ggtactgcgt ttgcttaaac ataaagctcg cgccagtgga actgcggtac tgttcatcag
     9061 ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt atgccggaag
     9121 cgtaattgaa agcggcgtga cggcagacgt tatccatcat ccccggcatc cgtataccat
     9181 tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagccattac ccgccattcc
     9241 agggacggta ccaaacctca cccatttgcc tgacggctgc gcttttcgcg atcgttgcta
     9301 tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg acaacaacca
     9361 gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt atctgacacg ttattaacgt
     9421 tacgcgacgt ccatatcaat ttcccggccc gtaaaagctg gcttggtaaa actacggaac
     9481 atgttcatgc gattaatggt attgatttac agatccgccg tggtgaaacc ttagggatcg
     9541 tcggtgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt atgctgcaac
     9601 cgagccacgg gcaatacatc cgttcaggat cacaacgcat tatgcagatg gtgtttcagg
     9661 acccgctctc ttcgcttaat ccgcgcttat cggtgtggcg catcatcaca gaaccgctct
     9721 ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt ggcaaaggag ttggctgtgc
     9781 aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc ggcgggcagc
     9841 ggcaacgcat cgccattgcc agagcactct cttcgcagcc tgacgtgatt gtgcttgatg
     9901 agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta ctggtaacgc
     9961 tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg gtgatacgtc
    10021 atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg ggcgacgcgc
    10081 agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat tccctccccg
    10141 ccattgataa accgctggag gaagaatggg cattatgtaa aacggatctg ccgggaaacc
    10201 gcacgttgcc acaaggctgt tttttctacg aacgttgccc tctggcaacc cacggatgtg
    10261 aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc tggcgggcgc
    10321 tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc cggcaatgct
    10381 ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag aaaccgggca
    10441 tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac ccggcaccgc
    10501 aacttcactc ttcagtgcga ttttcgtaat cgcaaaatcg gcatccactt tatccagcga
    10561 cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca gcattaatga
    10621 aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag ggttggttcc
    10681 tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca cgccactctg
    10741 ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct gacctttctt
    10801 atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac tacgtgagaa
    10861 acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt acttctgcac
    10921 ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaagagat attgcatcaa
    10981 tatcaatcaa ctcaccacaa taataataca tatcaatatc atggagttaa aatataaaaa
    11041 tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc tgaatgtgct
    11101 ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga atcactatgt
    11161 ttacttatta t
//
LOCUS       DAJTXQ010000127        11139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000127 DAJTXQ010000000
VERSION     DAJTXQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.127"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1212
                     /gene="narU"
                     /locus_tag="OO515_003302"
     CDS             <1..1212
                     /gene="narU"
                     /locus_tag="OO515_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6577154.1"
                     /translation="LNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWT
                     VFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQ
                     GSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVP
                     LLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAM
                     LAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFL
                     TLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQ
                     KEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTW
                     LVYGRRKFSQK"
     gene            1294..5034
                     /gene="narZ"
                     /locus_tag="OO515_003303"
     CDS             1294..5034
                     /gene="narZ"
                     /locus_tag="OO515_003303"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR6577155.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5031..6575
                     /gene="narH"
                     /locus_tag="OO515_003304"
     CDS             5031..6575
                     /gene="narH"
                     /locus_tag="OO515_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6577156.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6575..7270
                     /gene="narW"
                     /locus_tag="OO515_003305"
     CDS             6575..7270
                     /gene="narW"
                     /locus_tag="OO515_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6577157.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7267..7947
                     /gene="narI"
                     /locus_tag="OO515_003306"
     CDS             7267..7947
                     /gene="narI"
                     /locus_tag="OO515_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6577158.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8026..8919
                     /locus_tag="OO515_003307"
     CDS             8026..8919
                     /locus_tag="OO515_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR6577159.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9015..9860)
                     /gene="nhoA"
                     /locus_tag="OO515_003308"
     CDS             complement(9015..9860)
                     /gene="nhoA"
                     /locus_tag="OO515_003308"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR6577160.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10033..10602
                     /locus_tag="OO515_003309"
     CDS             10033..10602
                     /locus_tag="OO515_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR6577161.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10606..10833)
                     /gene="pptA"
                     /locus_tag="OO515_003310"
     CDS             complement(10606..10833)
                     /gene="pptA"
                     /locus_tag="OO515_003310"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCR6577162.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10933..>11139)
                     /locus_tag="OO515_003311"
     CDS             complement(10933..>11139)
                     /locus_tag="OO515_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6577163.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2589 a         2910 c         2975 g         2665 t
ORIGIN      
        1 ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac cgcattaccc
       61 tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat attcggtgga
      121 cgccgatgga cggtttttag tactgcaatc ctgattattc cttgcgtctg gctcggaatt
      181 gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt gctatgcggt
      241 tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc
      301 aaacaaggga gcgctcttgg gattaatggc ggattaggaa acttaggtgt aagtgtaatg
      361 cagctggttg caccgctggt catttttgta cctgtatttg cctttctcgg cgtcaatggc
      421 gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat ttgggtaccg
      481 ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc cagttcacgc
      541 gcctcaattg acgaccagct ccctgtctta caacgcctgc atctctggct gctgagcctg
      601 ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc catgctggca
      661 aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc atttatcggt
      721 gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt gcgggtgacg
      781 ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac cttaccgggc
      841 acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt tctgactgcg
      901 ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca gataaccatt
      961 tatcgggtga agatgaaagg cggtagtgat gagcaagctc agaaagaagc catcaccgaa
     1021 acggcggcgg ctctgggctt tatctcagcc attggcgcag tgggcggctt ttttattccg
     1081 caggcgtttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat gaaagtgttt
     1141 ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg gcggaagttc
     1201 agccaaaaat aagagatcga tgcctgatgt acaaccgtgt cgcatccgac aaggcatcag
     1261 atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga tcgctttcgc
     1321 tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat gcatagcaac
     1381 cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaattgt gcgttccacc
     1441 cacggtgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa tggtctggtg
     1501 acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgaccagcc caatcatgaa
     1561 cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tctacagcgc taaccgcctg
     1621 aaatacccgc tcattcgtaa acgactgatt gaactgtggc gtgaagctct caagcaacac
     1681 agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg cttaagctac
     1741 aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact taaccaactg
     1801 attgccgccg ctaacgtctg gaccatcaaa atctatggtc cggatcgcgt tgccggtttc
     1861 tcgccgatcc cggcgatgtc gatggtgtca tacgctgccg gaacgcgtta tctgtcgctg
     1921 ttaggcggca cctgtttaag cttctacgac tggtattgcg acctgccgcc cgcctcgccg
     1981 atgacctggg gcgaacaaac cgacgtgccg gaatctgccg actggtataa ctccagttac
     2041 atcatcgcct gggggtctaa cgtaccgcag acacgtacgc cggacgccca cttctttacc
     2101 gaagtacgtt acaaaggcac caaaactatc gccattaccc ctgactactc tgaagtggcc
     2161 aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct ggcgatggca
     2221 atgggccatg tgattttaaa agagtttcat ctcgataatc ccagcgacta ctttatcaac
     2281 tactgccgcc gctatagcga catgccgatg ctggtaatgc tggaacctcg cgacgatggt
     2341 agctacgtac ccgggcgcat gatccgcgcg tctgacctgg tagatggact aggcgaaagc
     2401 aataatccgc agtggaaaac cgtggcggtt aataccgcag gtgaattggt agtgccgaat
     2461 ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc cattgccgcc
     2521 ggtaaggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt tgcaggcgtg
     2581 gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa acacaatccg
     2641 gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa cacctgtccg
     2701 gtggtcagcg tttatgattt ggtgctggcg aattacggcc tcgatcgcgg gctggaagat
     2761 gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg gggtgagcaa
     2821 attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc cgataccgcc
     2881 cataaaacgc atggacgctc aatgattatc ctcggcgctg gcgtcaacca ctggtatcac
     2941 atggatatga actaccgtgg gatgatcaat atgctgatct tctgcggttg tgtcgggcaa
     3001 agcggcggcg gctgggcgca ctatgtcggt caggaaaaac tgcgcccaca aaccggctgg
     3061 ttgccgctgg cctttgcgct cgactggaac cgcccaccgc gccagatgaa cagtacctcg
     3121 tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca ggagttactt
     3181 tcaccgcttg ccgatgccag taagtacagc ggtcatctga ttgatttcaa cgttcgcgcc
     3241 gaacgcatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct cgggattaaa
     3301 gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc gctgaaatcg
     3361 ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc gcgtaatttg
     3421 tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta tatgcagaag
     3481 tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag cgacgggatc
     3541 aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga cctgctggtg
     3601 acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct gcccaccgcc
     3661 acctggtacg aaaaagacga tatgaacacc tcggatatgc atccgtttat tcatccgctt
     3721 tctgcggcgg tcgatccggc gtgggaatca cgcagcgact gggaaatcta caaaggtatc
     3781 gccaaagcat tttcgcaagt gtgcgtgggt catcttggca aagaaaccga cgtggtatta
     3841 caacccctac tgcatgactc tccggcagag ctctcacagc cgtgtgaagt gctcgactgg
     3901 cgcaaaggcg aatgcgatct gattccaggc aaaaccgcgc cgaatattgt ggcggtggag
     3961 cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat ggacaaactt
     4021 ggcaatggtg gtaaagggat ttcgtggaat acccaggatg aaatcgattt cctcggcaaa
     4081 ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga caccgccatt
     4141 gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt tgcagtcaaa
     4201 gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc gctgcacaaa
     4261 gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat tatctccagc
     4321 cccacatggt ccggtctgga aagcgatcac gtctcctata atgcgggata caccaacgtt
     4381 catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca ggatcatccg
     4441 tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga cacccgtagc
     4501 gtcagtgaga tgcgccagat cccgccaaac ggcttcccgg aaaaagcact taacttcctg
     4561 acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct aatgctgacg
     4621 ctctctcgcg gtggaccgat tgtctggatc agcgaaaccg atgcccgaga actgaccatt
     4681 gtcgataacg actgggtgga agtctttaac gccaacggcg cgctgacggc tcgtgcggtg
     4741 gtcagccaac gtgtaccgcc gggtatgacc atgatgtatc acgcccagga acgcattatg
     4801 aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc ggtcacccgt
     4861 gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg gggctttaac
     4921 tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa gatgaagaac
     4981 gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa atgaaaatac
     5041 gttcgcaagt cggcatggtg cttaacctcg acaaatgtat cggttgccat acctgttcgg
     5101 tgacctgtaa aaacgtctgg accggacgcg aaggcatgga gtacgcatgg tttaacaacg
     5161 tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag
     5221 gcggctgggt ccgcgatgtg aatggcaaga tacgcccgcg tctgggcagc aagatgggcg
     5281 taataaccaa aatcttcgcc aacccggtgg tgccgcagat tgatgattac tacgaacctt
     5341 tcaccttcga ctacgaacat ttgcatagcg caccggaagg caaacatatc cctactgctc
     5401 gcccgcgttc gctgattgac ggtaagcgga tggacaaagt gatctggggg ccaaactggg
     5461 aagaactgct gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc gaggccatgc
     5521 aaaaggagat gtacgggcag tttaaaaaca ccttcatgat gtacctgccg cgcctgtgcg
     5581 aacactgcct caatcccagt tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg
     5641 aagaagacgg cattgtgctg attgatcagg ataaatgccg tggctggcgt ttgtgcatta
     5701 gcggttgtcc gtacaaaaaa atctacttca actggaaaag cggcaagtca gaaaaatgta
     5761 tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa acctgcgtgg
     5821 gtcgcatccg gtatctgggc gtgctgcttt acgacgccga ccgcattgag gaagcggcga
     5881 gcaccgagcg cgaagttgac ctctatgaac gtcagtgcga agtgttcctc gatccacacg
     5941 atccctcagt gatcgaggaa gccctgaaac aaggtattcc acaaaacgtg attgaagctg
     6001 cccagcgttc gcctgtctac aaaatggcga tggactggaa actggcgcta ccgctgcacc
     6061 ctgaatatcg caccctgcca atggtctggt acgttcctcc gctgtcgccg attcagtcct
     6121 acgcagatgc gggcggtttg ccgaaaagcg aaggcgtgct gcccgccatc gaaagcctgc
     6181 gtattccggt gcaatatctc gccaatatgt tgagtgccgg cgataccggt ccggtactgc
     6241 gggcgctgaa acggatgatg gcgatgcgcc actatatgcg ttcacaaacc gtggaaggcg
     6301 ttactgatac tcgtgccatc gacgaagtag gcctgagcgt cgcccaggtc gaagaaatgt
     6361 atcgctatct cgccattgcc aactatgaag atcgttttgt tatcccgacc agccatcggg
     6421 aaatggcggg cgatgccttc gcagaacgca acggctgcgg ttttaccttt ggcgacggtt
     6481 gccacggttc ggacagtaaa ttcaacctgt tcaacagtag ccgtatcgat gccatcaaca
     6541 tcaccgaagt gcgcgacaaa gcggagggcg aataatgcag atcctcaaag tgatcggcct
     6601 gttgatggag tatccggatg agctgttgtg ggaatgcaag gaggacgcgc tggcgttgat
     6661 ccgccgcgac gcgccgatgc ttacggattt cacccacaac ctgcttaacg cgccgctgct
     6721 ggataaacag gccgaatggt gcgaagtgtt tgaccgcggg cgcaccacgt cgctgctgct
     6781 gttcgaacat gttcatgccg agtcccgcga tcgcggccag gcaatggtgg acctgctggc
     6841 ggagtatgaa aaggtcggcc tgcagctgga ttgtcgggaa ctgcccgatt atctaccgct
     6901 gtatctggag tatttaagcg tactgcctga cgatcaggcg aaagaaggat tgctcaatgt
     6961 tgcgccgatc ctcgccctgc ttggcggtcg cttaaaacaa cgcgaggcac cgtggtacgc
     7021 gttgtttgat gctctgctgc aactggcggg aagtatactt tcaagtgaca gtgtcacaaa
     7081 acaagtaaac agcgaagagc gcgatgacac ccgccaggcg cttgatgcag tgtgggaaga
     7141 ggaacaggtg aagtttattg aagataacgc cacggcgtgt gacagctcgc cgcttaatca
     7201 atatcagcga cgctttagcc aggatgtcgc gccgcagtat gtcgacatca gtgcgggagg
     7261 tgggaaatga ttcagtatct gaacgtcttt ttttacgata tctacccgta catttgtgcg
     7321 acggtgtttt tcctcggcag ctggctgcgc tacgactacg ggcagtacac ctggcgcgcc
     7381 tcctcaagtc aaatgctcga taaacgcggg atggtgatat ggtcgaatct gttccatatc
     7441 ggcattttgg ggattttctt cgggcacttg ttcggcatgt taacgccgca ctggatgtac
     7501 gcgtggtttt tgccagtggc agcgaaacaa ctcatggcga tggtgctcgg cggtatttgc
     7561 ggtgtgttga cgttgattgg cggcgcagga ctgctgtggc gcagtctgac aaatcagcgc
     7621 gtgcgtgcca cttccactac gccggatatc atcatcatga gcattttgct gatccagtgt
     7681 ctgttggggc taagtaccat accgttttca gcacagtatc ctgatggtag cgaaatgatg
     7741 aagctggtgg gttgggcgca aagcattgtc actttccgtg gtggatcgtc agagatgctc
     7801 aacggcgtag cgttcgtctt ccgcctgcat ctggtgttgg gaatgaccat cttcctgctc
     7861 ttcccgttca cccgactggt gcatgtgtgg agcgcgccgt ttgagtactt tactcgtcga
     7921 tatcaaattg tgcgttcgcg gcgataattt caccctggcc ggatagccct tcgctctccg
     7981 gccacttgca ggctatgata gttctccagt cacaggagaa cgttaatgaa accgcaggtg
     8041 taccacgtcg atgcttttac ctctcaacca tttcgcggca attctgccgg tgttgttttc
     8101 cccgccgata atcttagcga agcacaaatg cagcttatcg cccgcgagtt aggccattcg
     8161 gaaactgctt ttctgctgca cagcgacgac agcgatgtgc gcatccgcta ctttacgcca
     8221 acggttgaag tgccgatttg cggtcacgcg acagtagctg cgcactatgt acgtgcgaag
     8281 gtgttaggtt taggaaattg cacgatctgg caaacatcgc tggcaggaaa acatcgcgtg
     8341 actatcgaaa agcacaacga tgattatcgt atttcgctgg aacaaggtac gccgggcttt
     8401 gaactgccgc tggagggtga agtacgcgcg gcgattatca acgcattgca tcttactgaa
     8461 gacgatattc tgccgggttt gccgatccag gtcgcgacga ccgggcattc taaagtgatg
     8521 atcccattga agccggaagt ggatatcgac gctctttcgc ctgaccttaa tgcgctgacc
     8581 gctatcagta aacagattgg ctgcaatggt ttcttcccgt tccagatccg tccaggcaaa
     8641 aacgaaaccg atggtcgtat gttctcgcct gcgattggta ttgtggaaga tccggtgact
     8701 ggcaatgcca atggcccgat gggtgcatgg ttggtacatc acaacgtatt gccccacgat
     8761 ggcaacgtgt tgtgtgttaa aggccatcag gggcgcgcat tggggcgtga cggcatgatt
     8821 gaagtgacgg tgacaattcg tgataaccaa ccggaaaaag tcaccatttc aggcacagcg
     8881 gtgattttgt ttcatgccga atgggcaatt gaaatctgaa atcgttgcct gatgcgcttc
     8941 gcctgtcagg tctacaaccg ggctaatgta tgccggataa ggcgtttacg ccgcatccgg
     9001 caacctgaca taaattattt tcccgcctcc ggatgcgtat caaacgccgc catcaccagc
     9061 gctaactcat ccacagtaaa gccatgtttc gcatcatcca cgcccagacc aaactgttcc
     9121 tgcatcacgg catataacga cgccacatcc ggtagatttc gctgctccac cgcgtgccca
     9181 ttttcataat gggtaaaatg aaaattggtc agtgtcagct tgccgccgtc cggcaaatgg
     9241 cggcacatca gcaaatgatg gcgaaaatgc gactgcggcc agtgcgccga ccagaaattg
     9301 cccatcacat aatcgctttg ttgctgctcg cagagatcga agcggtacat cggctgccaa
     9361 tgctgatgat gattaaactg caacacccaa tcatcaccct cctgcaacaa ccgatactct
     9421 ccgtgtggcg tggtctgcac gagatcggaa actaaacgaa tcggcgcggt tagcgtctgc
     9481 ccaccgaaac cgacatcagc aatccatttt tccccttcca gttccaccaa cagcaaacga
     9541 tgggtgcgcg gcggtaatgc tggcggattt gataacacta cgcgccctaa caagctgcga
     9601 acgttaaacc ccagctcgcg taacacccgc tcaaacacgc cattctgctc aaaacagtaa
     9661 ccgccacgac gggctatcac cagtttctct tccagcgatt gatcatcaag ctgtatttcc
     9721 ctcggcagca aaacgtcaag gttttcaaac ggaatggtgc aattgtgttt caggtgcaat
     9781 gcacgaagcg tatcaatatt gaccgcagca gctcccgacc agttaatacg ggcaaaatag
     9841 tgattcagaa tgggcgtcat caggcgtttc ccttagtggt tttctcaact tataacctgg
     9901 aatagacgcc gcaccggcta ttttgtgcga tcgcggacat gcatcacgtt tgcaaatcgc
     9961 caatcccgtt aatttcgtta cttattgcgt tacggactac gcttaaaaaa accttcttcg
    10021 cgaggcgata ccatgagccg attcatcccc atagaattac accatgccag ccgtctgtta
    10081 aatcatggcc caactgtctt gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt
    10141 atggctgcag cctggtcaat gccggtggag tttgaacctc cacgcgtggc aattgtggta
    10201 gataaatcga catggaccag ggagttgatt gaacgtaacg gtaagtttgg catcgttatc
    10261 ccgggcgttg cagcaactaa ctggacgtgg gcggtgggaa gtgtgtcggt gcgtgaagaa
    10321 gataaattta attgctatgg cattccggtg gtgaaaggtc cggtgcttgg tttgccactt
    10381 gttgaagaga aatgcctggc gtggatggag tgccgcctgc tacctgcgac ttctgcgcaa
    10441 caaaaatacg acacgctgtt tggcgaggtg gtatcagcag cggcagacgc acgggtattt
    10501 gtcgaaggcc gctggcagtt tgatgatgat aagctcaata cgttgcatca tttaggtgct
    10561 ggcacgtttg ttactagcgg caagcgtgtt acggcaggtt aagcattaca tgctgtaacc
    10621 gggtttcttt atcaaagcct ccatttgggg ctcgatttcg gcatcccaga tagcttgcca
    10681 agattctggt tgaatctgct gtagagcaat gcttatcgag ctatctttac tgttcagatg
    10741 gcgaataata acgtcggtaa tatctgcagc aagtgctgct ttttgttgtt cgtccagttc
    10801 acgcggaaag catttaatgt cgatgtgcgg catgattacg ctccttttga aagatgtcag
    10861 taaaattaac acacattgtg aatgtcatgt gtccggtaaa gcgtttatac gacaataatg
    10921 cccagggcaa gattacgaaa gcccgctccc cgtaaggact gacgccaggt agtttctgcc
    10981 catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa
    11041 aatattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc ctcttcttat
    11101 tttgcagtcg tcttcattca ttaccacgtc cagacgcca
//
LOCUS       DAJTXQ010000128        11000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000128 DAJTXQ010000000
VERSION     DAJTXQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.128"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..277)
                     /locus_tag="OO515_003312"
     CDS             complement(<1..277)
                     /locus_tag="OO515_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577164.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="OO515_003313"
     CDS             complement(444..1184)
                     /locus_tag="OO515_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6577165.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OO515_003314"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="OO515_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6577166.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OO515_003315"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="OO515_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6577167.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OO515_003316"
     CDS             complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="OO515_003316"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6577168.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OO515_003317"
     CDS             complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="OO515_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6577169.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OO515_003318"
     CDS             complement(5776..6795)
                     /gene="epd"
                     /locus_tag="OO515_003318"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6577170.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7167..7598
                     /locus_tag="OO515_003319"
     CDS             7167..7598
                     /locus_tag="OO515_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6577171.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7621..8199
                     /locus_tag="OO515_003320"
     CDS             7621..8199
                     /locus_tag="OO515_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6577172.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8200..8907
                     /locus_tag="OO515_003321"
     CDS             8200..8907
                     /locus_tag="OO515_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6577173.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8895..9572
                     /locus_tag="OO515_003322"
     CDS             8895..9572
                     /locus_tag="OO515_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6577174.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9566..10243
                     /locus_tag="OO515_003323"
     CDS             9566..10243
                     /locus_tag="OO515_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6577175.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10215..10928)
                     /locus_tag="OO515_003324"
     CDS             complement(10215..10928)
                     /locus_tag="OO515_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6577176.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10925..>11000)
                     /locus_tag="OO515_003325"
     CDS             complement(10925..>11000)
                     /locus_tag="OO515_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6577177.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2626 a         2919 c         2741 g         2714 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3061 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3121 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3181 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3241 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3301 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3361 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat
     3421 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3481 tatcgatgtt catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3541 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg
     3601 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3661 aaacaccgtg tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca
     3721 gttcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3781 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3841 tgccgatttt ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3901 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt
     3961 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     4021 cagtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4081 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4141 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4201 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4261 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4321 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4381 catcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4441 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4501 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4561 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4621 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4681 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4741 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4801 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4861 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4921 gccgatgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4981 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     5041 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5101 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5161 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5221 gctgccaaca gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa
     5281 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5341 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5401 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5461 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5521 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt
     5581 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5641 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5701 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5761 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5821 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5881 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5941 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     6001 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6061 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6121 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6181 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6241 catggcggag tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa
     6301 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6361 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6421 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6481 ggagccatat acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag
     6541 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6601 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6661 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6721 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6781 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6841 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6901 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6961 aatgattgtc cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc
     7021 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7081 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7141 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg
     7201 cacgctttta tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7261 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7321 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag
     7381 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7441 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7501 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7561 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7621 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7681 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7741 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7801 accaatttac tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg
     7861 atggtcatcg gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca
     7921 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7981 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     8041 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg
     8101 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8161 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8221 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg
     8281 atctatagcg cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcgggca
     8341 aaatatgtcg cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8401 tggctaacag aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt
     8461 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8521 cccgtgcaat gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8581 ctgtttgccg gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8641 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8701 atgcccgcgt taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8761 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8821 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa
     8881 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8941 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     9001 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9061 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9121 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9181 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9241 aaacagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9301 gccatcgtca cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg
     9361 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc
     9421 aggccagcga aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga
     9481 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9541 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc
     9601 tagcgcagca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9661 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9721 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9781 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9841 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9901 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9961 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
    10021 actctctccg ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10081 gagcgtgttt gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat
    10141 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10201 tgtaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10261 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10321 taaaaggctt ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc
    10381 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10441 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10501 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10561 caatcccctt ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct
    10621 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10681 ccatccatcg ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc
    10741 cagaaggtgg ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10801 cgccgctgcg gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg
    10861 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10921 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10981 ccattccgct aaaacgcgcc
//
LOCUS       DAJTXQ010000129        10922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000129 DAJTXQ010000000
VERSION     DAJTXQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.129"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            149..490
                     /locus_tag="OO515_003326"
     CDS             149..490
                     /locus_tag="OO515_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6577178.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(481..1407)
                     /locus_tag="OO515_003327"
     CDS             complement(481..1407)
                     /locus_tag="OO515_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577179.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1497..2495
                     /locus_tag="OO515_003328"
     CDS             1497..2495
                     /locus_tag="OO515_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6577180.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2492..2710)
                     /locus_tag="OO515_003329"
     CDS             complement(2492..2710)
                     /locus_tag="OO515_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6577181.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2712..4727)
                     /gene="ligA"
                     /locus_tag="OO515_003330"
     CDS             complement(2712..4727)
                     /gene="ligA"
                     /locus_tag="OO515_003330"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6577182.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4798..5784)
                     /gene="zipA"
                     /locus_tag="OO515_003331"
     CDS             complement(4798..5784)
                     /gene="zipA"
                     /locus_tag="OO515_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6577183.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6014..6775
                     /gene="cysZ"
                     /locus_tag="OO515_003332"
     CDS             6014..6775
                     /gene="cysZ"
                     /locus_tag="OO515_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6577184.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6960..7931
                     /gene="cysK"
                     /locus_tag="OO515_003333"
     CDS             6960..7931
                     /gene="cysK"
                     /locus_tag="OO515_003333"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6577185.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8315..8572
                     /gene="ptsH"
                     /locus_tag="OO515_003334"
     CDS             8315..8572
                     /gene="ptsH"
                     /locus_tag="OO515_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6577186.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8617..10344
                     /gene="ptsI"
                     /locus_tag="OO515_003335"
     CDS             8617..10344
                     /gene="ptsI"
                     /locus_tag="OO515_003335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6577187.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10385..10894
                     /gene="crr"
                     /locus_tag="OO515_003336"
     CDS             10385..10894
                     /gene="crr"
                     /locus_tag="OO515_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6577188.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2659 a         2818 c         2707 g         2738 t
ORIGIN      
        1 cgaagcgagt catcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt
       61 gctatctgcg atttgtaaga ttgatttttc ttctcaagtt ccttgttctt ctgccgatat
      121 tacctcgtac agtgacgagg actgaacaat ggccactatc tcttcaactt caattccttc
      181 catacaaaca cagagtagta acagggcatc acaaggtagc gatgttgctt caaaaatcgc
      241 gcggataagt cagcaaatca tcaaactcac tcaacaaatt aaagagattg tagacactag
      301 cggcagtgct gaagacaaac aaaaacaggc cgaattgatt caacagcaaa tcaccctact
      361 ggaaacacaa ctggcgcagt tgcagagaca gcaggcagaa aaggcgcagg agaaagagca
      421 gcgtttgtct ctgaatgtat cccttctaaa tcctgttgaa aatacgactc atatcgatat
      481 ctatatctga tacaacggag ccccttcccg accagccatt agcgcattgc cctggtctcg
      541 taccacgtcc cataacgctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag
      601 catcaactgg cgttcaacaa ccggcgtaat gcgctttacc accagtgggc taccttcagg
      661 caatggtagc gccagtgcgg gaagaatact gatgccgatg cctgcggcta ccatcgggaa
      721 cagcgtcgcc ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag
      781 cgctgcgtca atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc
      841 ctgtaatgcc tgccagggta cataatcttc cacggccaaa gcgctatcgc gatggcaaag
      901 cagaaagaaa ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc
      961 gatgacgatg caaaaatcga catctccctg gcgaatactt tccatcaccc actgctgcgg
     1021 acgatcgtgc aggacaaact gaatatctgg atagcggcga tggctttcag caatgcattg
     1081 cggaataaga tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg
     1141 ttgccccata cgcccggtat cgcgcaacgt gctgttaagt tcatccagca gtcgctcaag
     1201 acgcaaagcc aactgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag
     1261 caggcgaaca ccagtatgat tttccagctc cttcacactg tgacttactg ccgactggct
     1321 caagccgata cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac
     1381 ctttaattgt tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa
     1441 ttttatttct caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga
     1501 aactttttcg tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct
     1561 ctttctttcc ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcta
     1621 ttgccctgct gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg
     1681 gtggtcactg gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc
     1741 tgggtgtact gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt
     1801 ttctctactt gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg
     1861 cgggcggtaa cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg ctggggattt
     1921 tcctttcacc attgctagtt ggtctggtga tgaatgttca cggtgcaggg ggcagccttg
     1981 agcaggtcgg taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc
     2041 ggccgtggat tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga
     2101 cgtccattct gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc
     2161 ataaagttgg ctggggatca ttgctgttta tcgtggtggt cagctgcgtt cttctggcta
     2221 tcgtgattgt agttaacgtc tttatggcac gccgactggg cttcaataag gcagatgaaa
     2281 ttactatcgt cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca
     2341 ttctgttccc cacatcggtg atcggtatga tggtgctgcc cctgatgatt ttccatcaga
     2401 tccaattgat ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac
     2461 aggcgcagca ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca
     2521 gttgcgtcag cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc
     2581 ctgccgggaa ctcatctttg cgggcaaacc acagcagata ctcctccggc aggtcgatta
     2641 agcgacgccc tttgtatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct
     2701 ctttttccat ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg
     2761 cccagttcct gcgccttcgc cagtttagat cctgcagctt cacccgctat caccagatcg
     2821 gttttcttcg acacgctgcc cgcgactttc gcgcccagtt cgaccagtcg agctttagcg
     2881 tcatcacgcg acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg
     2941 ctgtcaatct cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac
     3001 agctcgctga tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca
     3061 acgacaatgc caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcact
     3121 tccagcgtgc cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg
     3181 ccaagtgcat agaggaagcg agcaaaggtg gtttctttcg ctttttccag cgcgttaacc
     3241 acgttttgtg ccgattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt
     3301 ttgaacagat ctgccggagt gtggacatat tctttttcaa ccagctgatc gatgattttg
     3361 tcgcccattc cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta
     3421 cgctgcgcac cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc
     3481 tcaacgtcag aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc
     3541 gggcgttcag aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc
     3601 accactttat cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt
     3661 gcgttactca ccagcacgcc agcaacatgg acaggttcca gacgcgcaac aggcgtaatc
     3721 gcgccagtac gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc
     3781 gggaatttaa acgctaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc
     3841 tgttccagtg agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg
     3901 tcttcttcca ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc
     3961 cgatcgctga ccggcaaccc ccacttttta aattgcagta aacggccaag atgagtatcc
     4021 ggcagctcgc caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc
     4081 gctgtaatac gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca
     4141 aaaactttcc cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc
     4201 aggaacactt caccacgcac ttccagacgc gccgggatat tctctccgtg cagcttcagc
     4261 ggaatggcgc gaatagtacg cacattagac gtgatatctt ccccggtggt gccatcgcca
     4321 cgggtcgcgg cactgactaa aacgccattt tcatacagaa tactgacggc aaggccatcc
     4381 agcttcagct cacagcacca ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt
     4441 ttgttgaaag caagaaagct ttcttcatca aaaacgttat ccagtgacag cattggtact
     4501 tcatggcgta tctggctgaa agccgccagc ggcgcagcac ctacacgttg ggtaggcgaa
     4561 tcaggcgtaa tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg
     4621 tcgtattcag cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg
     4681 cgaagcgtcg ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa
     4741 tgctaaaaac ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca
     4801 ggcgttggcg tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt
     4861 catcatacgg cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc
     4921 agattgcagc atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa
     4981 gatagtgaca cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac
     5041 catattcgcc aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta
     5101 aatattcata tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc
     5161 gtttagctcg ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt
     5221 cggtttatcc ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg
     5281 ttctgcaggc tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc
     5341 tggcgcgacc tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc
     5401 aggctgctgc atcggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg
     5461 cggctgctgg atcggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg
     5521 cggcgacgga cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg
     5581 aacacgaacc tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc
     5641 acgttttgac ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg
     5701 gctggtccag aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa
     5761 tatcagacgc aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg
     5821 caaacattta ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact
     5881 ttcacagcac aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac
     5941 tgtccattgc gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga
     6001 gctcatcctg attatggttt catcattcac atctgcccca cgcagcggtt tttactattt
     6061 tgcgcaaggc tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct
     6121 gctggtcaat attttgttga tggggggcgc attctggtgg ctctttacac agctcgatgt
     6181 ctggatcccg actctcatga gttacgttcc ggactggcta caatggctga gttatctgtt
     6241 gtggcctctg gctgtcatct ctgtgctgtt agtgtttggc tatttcttct ccacgattgc
     6301 taactggatt gccgctccgt ttaacggttt attggctgaa caactggaag cacgattgac
     6361 tggcgctaca ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat
     6421 gaaacgcgaa tggcaaaaat ttgcctggta tctgccgcgc gcaattgtat tactaattct
     6481 ttacttcatc cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc
     6541 ctggatgtta gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa
     6601 agagatgcgc accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac
     6661 cagcctgttt acgatgatcc cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg
     6721 cgcgacggcg atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc
     6781 taccggttat tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc
     6841 catcttgcat gtcattattt cccttctgta tatagatatg ctaaatcctt acttccgcat
     6901 attctctgag cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca
     6961 tgagtaagat ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga
     7021 atcgcatcgg taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg
     7081 ttaagtgccg tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac
     7141 caggcgttga actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag
     7201 ctgccgctcg cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc
     7261 gcaagctgct gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga
     7321 aaggcgcaat ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc
     7381 tgcaacaatt cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga
     7441 tctgggaaga taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta
     7501 cgctgactgg cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg
     7561 tcgccgttga gccaaccgat tctccagtta tcgcccaggc gctggcaggt gaagagatta
     7621 aacctggccc gcataaaatt cagggtattg gcgctggttt tatcccggct aacctcgatc
     7681 tcaagctggt cgataaagtc attggcatca ccaatgaaga agccatttct accgcccgtc
     7741 gtctaatgga agaagaaggt attcttgcag gtatctcttc tggagcagct gttgccgcgg
     7801 cgttgaaact acaagaagat gaaagcttta ccaataagaa tattgtggtt attctaccat
     7861 catcgggtga gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaat
     7921 tgcaacagta atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt
     7981 tgtggcctgc ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat
     8041 ttaaccagac taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga
     8101 atcgatttta tgatttggtt caattcttcc tttaacggca taatgtttaa tgacgtacga
     8161 aacgtcagcg gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc
     8221 tgttaaaaac tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt
     8281 ccacaacact aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac
     8341 cgctccgaac ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt
     8401 cacttctgaa attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa
     8461 actgcagact ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga
     8521 cgagcagaaa gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg
     8581 gttcttttaa aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc
     8641 ccgggtatcg ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg
     8701 aaaaaaattt ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc
     8761 aaggcatcag cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa
     8821 aaagaagcca tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa
     8881 atcatagccc tgattaaaga taaacacatg acagctgacg cagctgctca tgaagttatc
     8941 gaaggtcagg cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct
     9001 gacgtacgtg atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac
     9061 ctgagcgcca ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc
     9121 gcacagctga acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc
     9181 cacacctcta tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc
     9241 acctctcagg tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac
     9301 gtcaatccaa ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct
     9361 gaaaaagcag agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag
     9421 gtagaagtat gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc
     9481 gctgaaggcg ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc
     9541 actgaagaag aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg
     9601 gttatcgttc gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg
     9661 aaagaagaga acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag
     9721 atcctgcgcg atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt
     9781 atgttcccga tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc
     9841 tacaaacagg aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta
     9901 atggtggaaa caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc
     9961 tttagtatcg gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat
    10021 atgatttcac acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt
    10081 attgatgctt ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat
    10141 gaacgtgcta cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt
    10201 tctatcccgc gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta
    10261 gcagagcagg ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc
    10321 attgaagaaa aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa
    10381 gatcatgggt ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg
    10441 aactattgag atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga
    10501 tgtcgttttt gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa
    10561 aatggtcgcg cctgtagacg gtaccattgg taaaatcttt gaaaccaatc acgcgttctc
    10621 tatcgaatct gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact
    10681 gaaaggcgaa ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac
    10741 tgtcattgaa tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt
    10801 tgttatctcc aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt
    10861 gggtgaaacc ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc
    10921 ca
//
LOCUS       DAJTXQ010000130        10893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000130 DAJTXQ010000000
VERSION     DAJTXQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.130"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..611
                     /locus_tag="OO515_003337"
                     /pseudo
     CDS             1..611
                     /locus_tag="OO515_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OO515_003338"
     CDS             608..1222
                     /locus_tag="OO515_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6577189.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO515_003339"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO515_003339"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6577190.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OO515_003340"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OO515_003340"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6577191.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OO515_003341"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OO515_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6577192.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OO515_003342"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OO515_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6577193.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO515_003343"
     CDS             complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO515_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6577194.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            6626..7729
                     /gene="proB"
                     /locus_tag="OO515_003344"
     CDS             6626..7729
                     /gene="proB"
                     /locus_tag="OO515_003344"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6577195.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7741..8994
                     /gene="proA"
                     /locus_tag="OO515_003345"
     CDS             7741..8994
                     /gene="proA"
                     /locus_tag="OO515_003345"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6577196.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9109..9184
                     /locus_tag="OO515_003346"
     tRNA            9109..9184
                     /locus_tag="OO515_003346"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9142..9144,aa:Thr,seq:cgt)
     gene            9790..9948
                     /locus_tag="OO515_003347"
     CDS             9790..9948
                     /locus_tag="OO515_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577197.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2574 c         2777 g         2782 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcataacttt gccatagaca
     6241 tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc agatgccaca
     6301 atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa
     6361 cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg
     6421 ttaaagatat attacagatt aataatattc ttaaaatgtg gtaatttatt aaatctgtaa
     6481 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
     6541 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
     6601 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
     6661 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatattg ttgaacttgt
     6721 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
     6781 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
     6841 acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
     6901 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
     6961 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
     7021 aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata acgataacct
     7081 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
     7141 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
     7201 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
     7261 cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
     7321 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
     7381 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
     7441 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
     7501 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     7561 catccgcatt tgcaacctcg aaggccgcga tatcgcccac ggcgtcagtc gttacaacag
     7621 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
     7681 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagctggccg
     7741 atgctggaac aaatgggcat tgctgcgaag caagcctcgt ataaattagc gcaactctcc
     7801 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     7861 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa
     7921 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgta
     7981 cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
     8041 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     8101 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     8161 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     8221 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     8281 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     8341 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     8401 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     8461 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     8521 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     8581 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     8641 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     8701 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     8761 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     8821 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     8881 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     8941 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     9001 ccgggtgatg caaaagtagc catttgattc ataaggccat tgacgcatcg cccggttagt
     9061 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     9121 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     9181 accattaaga aaattaataa cttagcttaa cttctctaag aaattgtatc ttctgttatc
     9241 acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat gggcgagtag
     9301 ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta
     9361 aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct
     9421 acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc
     9481 aatagtggat taaagcccac ctcttcaaaa cctaacttga tcgggaaaga atcaagctct
     9541 agcacggttt cgtccaccgg agtacaacgg gtgcctgata agagaagaac tgctaagact
     9601 tcatagcttt tgtgaaaaag ccctttttag tggatacgcc tacggcgctt attttttggt
     9661 ataaccatca aaggcgcttt atggctccta ttgtcttgaa aatgtatctt tacttgtatt
     9721 gtgttttatg cgtagtctcc ctgtgtagta tgtaacttgt taattttcat atgaatacag
     9781 aggcacacga tgaaaaacat tgcagcaatc aaacgtaata accgcaagat tcacgctcgt
     9841 aagttcctgt ctacgccaga aggaaaagcc tggctagagc gtaaacagag agagaacgaa
     9901 gaaagaaaac tccttagtga gttgaaatgg cttagagagg atttctgaaa atctccagaa
     9961 cgcaacagga cgcattctaa cgctgttttc attactcggt atagggatag gtgatgtgtg
    10021 tttaatgcgt ctgtgcgctt gttttgtgcc ttattttgca ttttcataat gcggaagaga
    10081 acacggaaaa ccttaaaatt aattattggg gtatttaagg ctattttcag gtgatttaag
    10141 ggtattaaac ttgatttaat tgattaaaat ataaacagat aattatgtat cttattgatt
    10201 tataaggtta tttttaagta aaggtaaata aaaaataaat agccataaat agctataaat
    10261 acatcttaat acctatatcc cttcccttat ggctagattg cgagaggtgc ttaacgcgat
    10321 tttcagcgcc ccccgatctg gaaaatccag aatccccaaa tatcagcaag gaattttctt
    10381 gcgtaatcaa catcgttttt acctattaaa tcattacaca gaaagccttt ctgtaatgtc
    10441 tctcactaaa ctttcactaa acacgagaat gatcggtttt atgtcggatc agatgctaga
    10501 aacagcacca cgtctcacaa gggctgtaag cgatgaaacc agtgtttatg cgggtgcagg
    10561 tcaaaatata ggccaaaatc catttaacat aataaatgtt atatgtgcta aggaacacat
    10621 ataacgcctt gaattgatgt atcagtggaa aagtgatgga ttttttacgt aggagcacga
    10681 aagggcttct attgactttt gtgtgctatg aaatataatt aaagtttttt gtttttattg
    10741 tgtgatgtgg tgggttacac aggggaatag attaaatggt gttctgttta tacaggttta
    10801 tttgttagat caacaatttg gcatttctaa acagatgttt gaacgttgcc aaatgtcttg
    10861 acaacacaag aaaagggctt gctatgccag gca
//
LOCUS       DAJTXQ010000131        10840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000131 DAJTXQ010000000
VERSION     DAJTXQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.131"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO515_003348"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO515_003348"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6577198.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO515_003349"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO515_003349"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6577199.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="OO515_003350"
     CDS             complement(2696..3388)
                     /locus_tag="OO515_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR6577200.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="OO515_003351"
     CDS             3518..5278
                     /locus_tag="OO515_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6577201.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5684..6541
                     /gene="focA"
                     /locus_tag="OO515_003352"
     CDS             5684..6541
                     /gene="focA"
                     /locus_tag="OO515_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6577202.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6596..8878
                     /gene="pflB"
                     /locus_tag="OO515_003353"
     CDS             6596..8878
                     /gene="pflB"
                     /locus_tag="OO515_003353"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6577203.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9070..9810
                     /gene="pflA"
                     /locus_tag="OO515_003354"
     CDS             9070..9810
                     /gene="pflA"
                     /locus_tag="OO515_003354"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6577204.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9903..10079)
                     /locus_tag="OO515_003355"
     CDS             complement(9903..10079)
                     /locus_tag="OO515_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6577205.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10179..>10840
                     /locus_tag="OO515_003356"
     CDS             10179..>10840
                     /locus_tag="OO515_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577206.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2791 a         2711 c         2567 g         2771 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta taaggttaaa
     5401 tatcggtaat ttgtatttaa taaatacgat cgatattgtt actttattcg cctgatgctc
     5461 ccttttaatt aactgtttta gcggaggatg cggaaaaaat tcaactcatt tgttaatttt
     5521 taaaatttat ttttattttg ataatcaaat gtttactccg tatctgcata aaaaccatgc
     5581 gagttacggg cctataagcc aggcgagata tgatctatat caatttctca tctataatgc
     5641 tttgttagta tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc
     5701 ttttgatctt ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa
     5761 agcaacgaaa catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc
     5821 aatcgcattc gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat
     5881 ggcaaaactg gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg
     5941 agccgatctc tttacttcca ccgtgttgat tgttgttgct aaggcgagtg ggcgcatcac
     6001 ctggggtcag ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact
     6061 gctgtttgta cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg
     6121 actaaacgtc ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg
     6181 tcttggtatc ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg
     6241 cagcctgatg gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg
     6301 ttttgagcac agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt
     6361 cgcatccccg gaattttgga ccgcagtcgg ttctgcaccg gaaaattttt ctcacctgac
     6421 cgtgatgaat ttcatcactg ataacctgat tccggttacg atcggcaaca ttatcggtgg
     6481 tggtttgttg gttgggttga catactgggt catttacctg cgtgaaaacg accaccatta
     6541 atggttgtcg aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc
     6601 cgagcttaat gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa
     6661 tgaagtaaac gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc
     6721 cttcctggct ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt
     6781 taaactggaa aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat
     6841 cacctctcac gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac
     6901 tgaagctccg ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc
     6961 ctgcaaagcg tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg
     7021 taaaactcac aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa
     7081 atctggtgtt ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta
     7141 ccgtcgcgtt gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac
     7201 ttctctgcag gctgatctgg aaaacggcgt aaacttggaa cagactatcc gtctgcgcga
     7261 agaaatcgct gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg
     7321 ctacgacatc tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg
     7381 ctacctggct gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac
     7441 cttcctggat gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc
     7501 gcaggaaatg gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc
     7561 ggaatacgat gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat
     7621 gggcctcgac ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta
     7681 caccatgggt ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct
     7741 gaacttcaag aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa
     7801 cgatgacctg atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag
     7861 cccgatgatc gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac
     7921 catgctgtac gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa
     7981 gtctgaaccg atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca
     8041 cttcatggac tggctggcta aacagtacat cactgcacta aacatcatcc actacatgca
     8101 cgacaagtac agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac
     8161 catggcgtgt ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata
     8221 tgcgaaagtt aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg
     8281 cgaatacccg cagtttggta acaacgatcc gcgtgtagat gacctggctg ttgacctggt
     8341 agaacgtttc atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca
     8401 gtctgttctg accatcactt ctaacgttgt gtatggtaag aaaacgggta acaccccaga
     8461 cggtcgtcgt gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca
     8521 gaaaggtgca gtagcctctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga
     8581 tggtatctcc tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg
     8641 taagaccaac ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg
     8701 tggtcagcac ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa
     8761 cccggaaaaa tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc
     8821 gctgactaaa gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt
     8881 agatttgact gaaatcgtac agtaaaaagc gtacaataaa ggctccacga aagtggggcc
     8941 ttttttagca cgagagcctt ttttgtcagc tatctatact ctaaggtgac tgccaaaaca
     9001 gactcgacgt agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga
     9061 aacaccgcaa tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac
     9121 ggcccgggta ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt
     9181 cataaccgcg acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg
     9241 aaagaggtgg tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc
     9301 ggcggtgagg caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa
     9361 gaaggcattc atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt
     9421 gatgaactgc tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag
     9481 atccaccaaa atctggttgg agtttccaat caccgcacgc tggagttcgc caaatatctg
     9541 gcgaacaaaa atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat
     9601 gacgattcag cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc
     9661 gagcttctcc cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac
     9721 aaactcgacg gtgttaaacc accgaagaaa gagaccatgg aacgcgtgaa aggcattctt
     9781 gagcagtacg gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt
     9841 tgccgctttt ttattccgca cttcattctg ctacagcgtt tttaacacat cttatagtgc
     9901 atctaccata tcccgtacct gagataataa cgactgataa tattacgccg atataaatcg
     9961 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatatgt
    10021 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
    10081 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa taatgtaaac
    10141 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
    10201 ttgccacttt ctttgctgtg gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
    10261 gcttatcctc gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc
    10321 gcttattcaa tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg
    10381 aacgattgcg cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc
    10441 gtctgacacc aacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt
    10501 taatgccttt actggttggt ttcacacgtg aatatcctga tattaaagtc gagctcacaa
    10561 ctgacgactc tcttgtcgat atcgtccaac agggctttga cgctggcgtt cgacttagtg
    10621 gcatcgtcga aaaagatatg atcagcgtgg cgatcggccc accagtgaaa ttatgtgtag
    10681 cagcaacgcc agagtacttt gcaagatatg gcaaaccccg ccatcctcat gatctgctta
    10741 atcatcaatg tgttgtattt cgttatccca gcggcaaacc tttccactgg caatttgcaa
    10801 aggagcagga aattgctgtc gcagggaata tcattctgga
//
LOCUS       DAJTXQ010000132        10721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000132 DAJTXQ010000000
VERSION     DAJTXQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.132"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..731
                     /locus_tag="OO515_003357"
     CDS             <1..731
                     /locus_tag="OO515_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577207.1"
                     /translation="KKKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDD
                     NGKIIFNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIG
                     WRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATA
                     IGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQ
                     QELDKWKRENMTPFMYVLNTPPVI"
     gene            741..1220
                     /locus_tag="OO515_003358"
     CDS             741..1220
                     /locus_tag="OO515_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577208.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1357..2142)
                     /locus_tag="OO515_003359"
     CDS             complement(1357..2142)
                     /locus_tag="OO515_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577209.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2472..2548)
                     /locus_tag="OO515_003360"
     tRNA            complement(2472..2548)
                     /locus_tag="OO515_003360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2512..2514),aa:Asp,seq:gtc)
     gene            complement(2681..3412)
                     /gene="dnaQ"
                     /locus_tag="OO515_003361"
     CDS             complement(2681..3412)
                     /gene="dnaQ"
                     /locus_tag="OO515_003361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6577210.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3477..3944
                     /gene="rnhA"
                     /locus_tag="OO515_003362"
     CDS             3477..3944
                     /gene="rnhA"
                     /locus_tag="OO515_003362"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6577211.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3941..4663)
                     /locus_tag="OO515_003363"
     CDS             complement(3941..4663)
                     /locus_tag="OO515_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6577212.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4697..5452
                     /gene="gloB"
                     /locus_tag="OO515_003364"
     CDS             4697..5452
                     /gene="gloB"
                     /locus_tag="OO515_003364"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6577213.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5524..6882
                     /gene="mltD"
                     /locus_tag="OO515_003365"
     CDS             5524..6882
                     /gene="mltD"
                     /locus_tag="OO515_003365"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6577214.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6931..7527)
                     /locus_tag="OO515_003366"
                     /pseudo
     CDS             complement(6931..7527)
                     /locus_tag="OO515_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(7558..8358)
                     /locus_tag="OO515_003367"
     CDS             complement(7558..8358)
                     /locus_tag="OO515_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6577215.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8599..9513
                     /locus_tag="OO515_003368"
     CDS             8599..9513
                     /locus_tag="OO515_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577216.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9510..10313)
                     /gene="dkgB"
                     /locus_tag="OO515_003369"
     CDS             complement(9510..10313)
                     /gene="dkgB"
                     /locus_tag="OO515_003369"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6577217.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10476..10552)
                     /locus_tag="OO515_003370"
     tRNA            complement(10476..10552)
                     /locus_tag="OO515_003370"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10516..10518),aa:Asp,seq:gtc)
BASE COUNT         2966 a         2450 c         2429 g         2876 t
ORIGIN      
        1 aaaaaaaaaa aaaaatactc agcccaataa catcatttga ttacattcat tttatcacta
       61 cacatccttc aggtataaaa gatacactcg cctggttagt taacgcgagt aaattaatga
      121 ctgagtttga tgacaatggg aaaattatat ttaacattaa tgccttaaag tatacaaaag
      181 caagttactt tgaaatactt ggagagaaat atataaaaat cacaacatcc agcccctggc
      241 ttttagaaaa gctcggtaaa tatattttct cttcacgagc gcctcaagtt ctggaactcg
      301 ctattggctg gcgaggagct ttatctgaat caataaaagg agttaagttc tgtatctggt
      361 tttcagtagc ctggagaacc atagaattta ttatgagttc agagcgagat ctggttaatt
      421 ttcttggcga tttttcaatg gatgtggcca aagcagttat agccggtggt gttgcaaccg
      481 ctattggaag cctggcttct tttgcctgtg ttagctttgg ctttccagta attcttgtcg
      541 gaggagcaat tttactgaca gggatagtgt gtacagttgt tttaaatgaa atcgatgctc
      601 aatgccattt atcagaaaaa ttaaaatatg caattagaga tggactaaaa cggcaacagg
      661 aacttgataa atggaaaagg gaaaacatga ctccatttat gtatgttctt aacactccac
      721 ccgtgatatg aggattaacc atgcgtttac atgttaaatt aaaagagttt ctttcgatgt
      781 tttttatggc catattattt ttccctgcat tcaatgcttc tttgtttttt accggagtta
      841 aacctctata ttcaataata aaatgctcca cggaaatatt ttatgattgg agaatgttga
      901 ttctgtgctt tggtttcatg tcgttttcct ttttaaatat tcatgtaatt ttacttacga
      961 taataaaatt attcttaatc aaaaaaacaa aggtagtcaa tttcgccaca gacataacaa
     1021 tacagctaac tcttatattt ttactaatag ctatcgtaat agcaccttta atagctcctt
     1081 ttgtcacagg atatgtcaat gctaattatc acccctgcgg taataatacg ggaattttcc
     1141 caggagcaat ctatattaaa aatggcatga aatgtaataa tgaatatatt tctcgcaagg
     1201 aagattcagc agttaaataa ttaaacattc taaaacaaac agtttttaac tccggattcc
     1261 atgtcctttc ttgcactgtc tttgtcaaga aaaatttctt atgcaaagaa ttttgtatta
     1321 tttccgccag aaattcctgg cggcagatat aatttattat tgatagcggt taaccaaatc
     1381 ttttaccatc gctttatagg tcttttgctt aagttgcatg atttgctgct ctgtcatatc
     1441 agtcgtactg gcttcattct tgtcgggcaa tggtggaagc actttatatt cataattcac
     1501 cggcgaccag gcagcccact cacctgtagc tacatccacc agcgtgaatc gtaccagata
     1561 gcgcagtgac atcgtgtcag tcaatttttg atttttataa tctgaccaaa ctattgattt
     1621 caatccagag tcatattttc ctgtctgcaa cgtatcctgg aaaacaataa tcgctttctg
     1681 atgccctttt gcagccacaa aacgcagtgc ctgcatccag ttcatattct cagcactggc
     1741 aacatcctcg ttctcatttt tatctttgtt tttgttacaa gtcagaggct tttgcctgtc
     1801 cgggatacca gagaatgtgg aaacagtata atattttcgc atctcctcct gcataatggt
     1861 ttccggtgcg cggttgccgg attgaacaag tattacagga gaattaaggg gaacgcggaa
     1921 ctcatctcca tcaagcgcag cttgaatatc ctcttcggat acggccagag tggtttcatt
     1981 accgaataaa tctttgtctg tcagttgctg gggagtgttt ttattaatat tactttgtgt
     2041 gacttgagct tttttcttct tttcatcaat agaagattca gagacacatc ctgcaagcag
     2101 cgagaataat acacatatac aacaaaccct tcttaaattc ataatcggac actccctcgc
     2161 ctgtcataat catggtaatg tttagctaac gatttttaat tatatactcc acttgaatca
     2221 gaacaaacaa aaataacagt atatataaat tcattaaatc aatagaaaaa tgaaaatcat
     2281 aacattcaaa tatatttaaa ttgaatgatt tgatagtatg attaaatatt aatgatatgg
     2341 tctaccatat gtgattaaca catttacagc ataacaaaac catagaccga agaaatagcg
     2401 ggatttaaat gcaaaaaaac cgcaaaatta aaaatcttgc ggttctctga actcattttc
     2461 atgagtgaat ttggcggaac ggacgggact cgaacccgcg cccccctgcg tgacaggcag
     2521 gtattctaac cgactgaact accgctccgc gttgtgttcc gttgggaacg aggcgaatat
     2581 tacggattgc ctcacccttc gtcaacggtt tttctcatct tttgaatcgt ttgctgcaaa
     2641 aatcgcccaa gccgctattt ttagcgcctt ttacaggtat ttatgcccgc cagaggcagc
     2701 ttccgccctt cttctccacc agatcaagac gggcttcatg agctgcaagc tcttcatctg
     2761 tcgcaaaaac aacgcgtaac ttacttgcct gacgtacaat gcgctgaatt gttgcttcac
     2821 cttgttgctg ctgtgtctct ccttccatcg caaaagccat cgacgtttga ccaccggtca
     2881 tcgccagata aacttccgca aggatctgtg catcgagtaa tgccccgtgt agcgttcgtt
     2941 tactgttatc tatttcgtag cgggcacata acgcatcgag gctgttgcgc ttaccaggaa
     3001 acattttcct cgccaccgca aggctatcgg tgaccttaca gaaggtattg gtcttcggaa
     3061 tatcgcgctt aagcaacgaa aactcgtagt ccataaagcc gatatcgaac gctgcgttat
     3121 ggatcaccaa ctccgcgccg cggatatagt ccatgaactc atcggctact tcggcaaacg
     3181 tgggcttatc gagcaaaaat tcatcggcaa taccatgtac gccaaaggct tccggatcca
     3241 ccagccgatc gggtttgaga taaacatgga agttattgcc cgtcaggcga cggttcacca
     3301 cttcaacggc accaatctca atgatcttgt ggccttcata gtgcgcacca atctggttca
     3361 taccggtggt ttcggtatcg agaacgatct ggcgtgtaat tgcagtgctc atagcggtca
     3421 tttatgtcag acttatcgtt ttacagttcg attcaattac aggaagtcta ccagagatgc
     3481 ttaaacaggt agaaattttc accgatggtt cgtgtctggg caatccagga cctgggggtt
     3541 acggcgctat tttacgctat cgcggacgcg agaaaacctt tagcgcaggc tacacccgca
     3601 ccaccaacaa ccgtatggag ttgatggccg ctattgtcgc gctggaggcg ttaaaagaac
     3661 attgcgaagt cattttgagt accgacagcc agtatgtccg ccagggtatc acccagtgga
     3721 tccataactg gaaaaaacgt ggctggaaaa ccgcagacaa aaaaccagta aaaaatgtcg
     3781 atctctggca acgtcttgat gctgccctag ggcaacatca aatcaaatgg gaatgggtta
     3841 aaggccatgc cggacacccg gaaaacgaac gctgtgatga actggctcgt gctgcggcaa
     3901 tgaatcccac actggaagat acgggctacc aagtcgaagt ttaagcttgt ggtttacgac
     3961 attgccgggt ggctccaacc gcctggcgaa ttcgtggctt gtttttactc tgtttcatcg
     4021 ggtttagcgt taaaggaata gtccgtttcc gggcaacaat aagttgtaag caaccaagcg
     4081 caggaatatg cgcattcaat agttttcctc cgtgtttgtt ccacgggaga acgtggaaac
     4141 ggctggcgtg cagcacttca aaattcaaca aagagagcca gtccagttgc cgcatcagag
     4201 taaacatccg gctgttatag ggcgaggttt ttcgcaatac cggcacaagt ttgcgtaatc
     4261 ccattaaact aatgggattg aagccactaa tcaccagcca gccatcatca atcaataccc
     4321 gatcggtttc acgcaataaa cgatgcggat cggtgcacca cggcaatgta tgtgccagta
     4381 gacaaacatc aacggattta tcggcaaaag gaagatgaag tggatccgcc tgcacctgga
     4441 cgggcattcc ttgcgcagaa acattcactt gatgagaaac cgcgcacgct tcgcaattga
     4501 tttctgcgct taaattgcca atcttaagca gatgaaaacc atacatttta gtgaaccacg
     4561 ggttgagctg gcgctccagc gccttgcgat aaagctctcc ccagggcaaa tcgccccagc
     4621 gatcaggagc cacgacagtt tgagggactc ttgccggttt catcacaacc ttccgtttca
     4681 cactaagagg taatctatga atcttaacag tattcccgcc tttgatgaca attacatctg
     4741 ggttttgaat gatgaagcag gtcgctgcct gattgtcgat cccggagacg cagagccagt
     4801 attaaacgcc attgccgcca ataactggca accggaggcc atatttctca cccaccatca
     4861 tcacgatcac gttggcggcg taaaagaact ggtggaaaag tttccgcaaa ttgtggtgta
     4921 tggtccacaa gagacacaag ataagggaac aacacaggta gtcaaagatg gcgaaactgc
     4981 cttcgttttg gggcatgaat ttagtgtaat tgccacaccg ggtcacactt taggacatat
     5041 ctgttacttc agtaaacctt atctattttg cggcgacacg ctgttttctg gtgggtgtgg
     5101 taggttgttt gaagggacag catcacaaat gtatcaatca cttaataagt taagtgcgtt
     5161 acctgacgat acattggtat gttgtgctca tgaatatacg ttatcaaata tgaagtttgc
     5221 tttgagtatt cttccgcacg atttgtccat aaatgattat tatcgtaaag ttaaggagtt
     5281 acgggcaaaa aatcaaataa cactacccgt aattctgaaa aatgagcggc aaattaatgt
     5341 ttttttaaga acggaagata ttgatttaat taatgtaatt aatgaagaaa cattattgca
     5401 acaacctgaa gagcgttttg catggttaag gtcaaagaaa gataggttct gataaaactt
     5461 tcttgtcatc ggctccgttc gccgttatga tcggtcgtct tttaagcaac tattgacaca
     5521 cacatgaagg caaaagcgat attactcgcc tctgtcctgc tcgtgggttg ccagagtacc
     5581 ggcaacgttc aacagcacgc acagagcctt tctgcagctg gtcaagggga agcagcaaag
     5641 tttacaagtc aggcacgatg gatggacgat gggacgtcta tcgcgccaga tggtgacttg
     5701 tgggctttca ttggcgacga gctaaagatg ggaattccgg aaaatgaccg gattcgcgaa
     5761 cagaaacaga aatatttacg caataagagc tatctccacg atgtaacttt acgggcagag
     5821 ccgtatatgt actggatagc agggcaagtt aaaaaacgta acatgcctat ggaactggta
     5881 ctactaccca tagtggagag cgcttttgat cctcacgcaa cgtctggcgc caatgccgca
     5941 ggcatctggc agatcattcc gagcacgggg cgcaattatg gtttgaaaca gacccgcaat
     6001 tatgacgcgc gtcgcgatgt tgttgcttca acaactgccg cgctgaatat gatgcagcgt
     6061 ctgaacaaaa tgtttgacgg cgactggctt ctgaccgtag cggcttataa cagcggcgaa
     6121 ggtcgggtca tgaaggcaat taaaacgaac aaagcgcgtg ggaaatccac ggacttctgg
     6181 tcgttaccgt tgccgcagga aacgaagcag tacgtgccta aaatgctggc attgagtgat
     6241 attctcaaaa acagcaagcg ttatggcgta cgtctgccaa cgaccgatga aagccgtgct
     6301 ctggcgcgtg tgcacctgag tagcccggtt gaaatggcga aggttgcaga tatggcgggg
     6361 atttccgtca gcaagctgaa gacattcaac gctggcgtga aaggctccac gctgggcgca
     6421 agtgggccgc agtacgtgat ggtgccaaag aagcatgcag atcaactgcg tgaatctctc
     6481 gcttcaggcg aaattgctgc tgtacagtcg acgctggttg ccgacaatac gccgcttaac
     6541 agccgtgttt acaccgtacg ctctggcgac acgctttcaa gcatcgcttc acgtctcggc
     6601 gtaagcacca aagatttgca gcagtggaac aaactgcgcg gatctaagct gaagccaggc
     6661 caaagtttga cgattggtgc aggcagtagc gcacagcggt tggcaaacaa cagcgatagc
     6721 attacgtatc gtgtgcgcaa aggcgattcg ctttcaagca ttgctaaacg ccacggcgtg
     6781 aacatcaaag atgtgatgcg ctggaacagc gacactgcga atctgcaacc aggcgataag
     6841 ctgacgttgt ttgtgaaaaa caacaacatg ccagattcct gacaaaccag ataataaaaa
     6901 aggcaccgat tcccccggtg ccttttttat ttatgccgct ttatgtgctt ctaccatgat
     6961 gatatcactg gtgaaagagc catcagtctg caaggaaaaa tacgttctca cctctgtcga
     7021 tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac gcattctcac
     7081 gacccatgaa gaaaattcca gcggtaactt atctgtaatt aaattatcaa ctatcagatt
     7141 ggcttcattg attaacgtca accacgcacc gctggcgtag tttcgtacgt gagaggtatc
     7201 gcgtaatgct tctaccgcct gtaaccagat gtcgcgcact gggtgacccg gagacattac
     7261 atccatcaca atcagcctac cgccaggttt caatatccta ttcacttctc gcagtgctgc
     7321 accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa atgcgttatc
     7381 ggcaaatggc agacttgcgg catatccctg gcgggtggtg atatttttca gttgccgggc
     7441 ttcggcggct tgtgccacga catccagcat gtgggcagat aagtcatacg ccaccaccgc
     7501 gctcacgttt tgcgcagcgc caaacgttgg tctgccctgc ggcagacctg acatacctta
     7561 tttatcaggc ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt
     7621 aaccagtaca gaagcttcac tgacgttcag accacggtag aaaacaaaat cgagcgggcg
     7681 accaaacgcc cgacggcgct gatcatcggt aaaacgcacc tggcgcagcg acatttcccg
     7741 cgcaaagcga tataacgcgt tcatccttct acggctccag gcattgaaat ctcccgccat
     7801 aatgaccggg ccgctgtggt gagctatctg atcaccaata ggaagtaact gcttactata
     7861 gacatccacg cccagactga agttgacggc gtgtatatta accaccatca acaggcgggt
     7921 gtcaggtaat ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg
     7981 ttcacgtaac gggcagcaat acactggatg tgccgccgaa agggtcatta cgccagaagg
     8041 atgttgtggc agcacgaaag cgggtacctg atcggcggca agatagttag cggtcgcaaa
     8101 ctgtaccaac tctggcgttg tttgcgcttc ctgcaataac accagatgtg cgtctttgcc
     8161 gtaattcttt aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag
     8221 gatccgaata cgctcttcgg tacttaacgg ttctccgggt ggtaatgcct ggccgatgct
     8281 cgcaaaagac cccggcggta agatcctctc cgcaggttgt ccggcaacat agcgcatggc
     8341 ataggtgttt tttcgcactt ttgactttga aacctctatt acgtcaaccc ttccgcactc
     8401 gggagggttc tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg
     8461 ttatgtaagc aagtgcttac accggtcaga aatttgctca ttattgagaa cattaaatcg
     8521 gatgacatat cacgcatcct gatggtttaa tgacaaataa attcaaaata tctcatctat
     8581 tttgcaacag gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcggtc
     8641 gtcgaaagcg gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta
     8701 agccgggcgg tgaaaaagct ggaaatgaaa cttggcgtta gcctgcttaa tcggaccacg
     8761 cggcaactta gcctgacgga agaaggcgag cgttattttc gtcgcgtaca gtcaattttg
     8821 caggagatgg cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg
     8881 ttacggatcg atgccgcaac tccagtggtg ctgcactttc tgatgccgtt aattaagcct
     8941 ttccgtgaac gctatccgga agtcacttta tcgctagtct cctccgaaac gattattaat
     9001 ttgatcgaaa gaaaagtgga tgtcgcgata cgcgctggta cgttaacgga ctccagctta
     9061 cgtgccaggc cgttatttaa cagttatcga aaaattatcg cctcccccga ttatatttcc
     9121 cgctacggga agccagaaac gatcgacgat ttaaagcaac atgtttgcct gggattcact
     9181 gaacccgctt ccctcaatac ctggccgata gcctgtagtg atggacaatt acatgaggtg
     9241 aagtacggtt tgtcatccaa tagtggggaa acactgaaac agctttgcct gagtggcaac
     9301 gggattgcgt gtttgtccga ctacatgatc gacaaagaaa tcgctcgcgg agaattggtg
     9361 gagttaatgg cagataaagt gttgccagtg gaaatgccat tcagtgccgt ctattacagc
     9421 gaccgtgcgg taagtacgcg catccgggct tttatcgatt tccttagcga gcatgtaaaa
     9481 acagctcccg gaggagctgt cagagaggct taatcccatt caggagccag accttccggg
     9541 ctaaccaggc ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc
     9601 tgtaaatttt gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt
     9661 actgagtaac cttcccccat agcccacgcc agaatcactt gtgccggagt cgcattgtgt
     9721 ttagctgcaa tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata
     9781 taggaagtga tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga
     9841 taaggagaga gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct
     9901 ttttccatca acgggatcgt gaagttggaa ataccgatct cacgtgtcag accttgtttt
     9961 ttggcctcca gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc
    10021 cagtggatta gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctcttttaga
    10081 ctcgggatca atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt
    10141 tcatgacgtg gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag
    10201 atttgtgcag tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata
    10261 acaacgtcgt ctttgagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc
    10321 ctcttttaat gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa
    10381 agagcataaa atgcagagga tttttgcgat tctggcaata atagatatac agacgaaaaa
    10441 aaacctcgag cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc
    10501 cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt
    10561 cttttacaac caccggtttt atgaccggct tactgcttaa tttgatgcct ggcagtttat
    10621 gaatcacttc gggctgttgc ttcgcaacgt tcaaaagctt tcgcttttgt cctactctct
    10681 tatcgagtag tgaactgcct caaaggtcag tgccattctt c
//
LOCUS       DAJTXQ010000133        10687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000133 DAJTXQ010000000
VERSION     DAJTXQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.133"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..784)
                     /locus_tag="OO515_003371"
     CDS             complement(<1..784)
                     /locus_tag="OO515_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577218.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OO515_003372"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OO515_003372"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6577219.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OO515_003373"
     CDS             1860..2747
                     /locus_tag="OO515_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6577220.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OO515_003374"
     CDS             2780..3790
                     /locus_tag="OO515_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6577221.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OO515_003375"
     CDS             3860..5293
                     /locus_tag="OO515_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6577222.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OO515_003376"
     CDS             5392..6741
                     /locus_tag="OO515_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6577223.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OO515_003377"
     CDS             6731..7618
                     /locus_tag="OO515_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6577224.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OO515_003378"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OO515_003378"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6577225.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OO515_003379"
     CDS             8352..9224
                     /locus_tag="OO515_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6577226.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OO515_003380"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OO515_003380"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6577227.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="OO515_003381"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="OO515_003381"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6577228.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2533 c         2854 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAJTXQ010000134        10591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000134 DAJTXQ010000000
VERSION     DAJTXQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.134"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO515_003382"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO515_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6577229.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OO515_003383"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OO515_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6577230.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIEGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OO515_003384"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OO515_003384"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6577231.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OO515_003385"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OO515_003385"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6577232.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OO515_003386"
     CDS             complement(5254..6294)
                     /locus_tag="OO515_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6577233.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OO515_003387"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OO515_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6577234.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OO515_003388"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OO515_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6577235.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OO515_003389"
     CDS             7229..7669
                     /locus_tag="OO515_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6577236.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OO515_003390"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OO515_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6577237.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OO515_003391"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OO515_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6577238.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10591
                     /gene="rsxC"
                     /locus_tag="OO515_003392"
     CDS             8898..>10591
                     /gene="rsxC"
                     /locus_tag="OO515_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6577239.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2428 a         2736 c         2704 g         2723 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat tgaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAJTXQ010000135        10531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000135 DAJTXQ010000000
VERSION     DAJTXQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.135"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1392)
                     /gene="fumB"
                     /locus_tag="OO515_003393"
     CDS             complement(<1..1392)
                     /gene="fumB"
                     /locus_tag="OO515_003393"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6577240.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKG"
     gene            complement(1470..2810)
                     /gene="dcuB"
                     /locus_tag="OO515_003394"
     CDS             complement(1470..2810)
                     /gene="dcuB"
                     /locus_tag="OO515_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6577241.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(3381..4100)
                     /gene="dcuR"
                     /locus_tag="OO515_003395"
     CDS             complement(3381..4100)
                     /gene="dcuR"
                     /locus_tag="OO515_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6577242.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(4097..5728)
                     /gene="dcuS"
                     /locus_tag="OO515_003396"
     CDS             complement(4097..5728)
                     /gene="dcuS"
                     /locus_tag="OO515_003396"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6577243.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            5909..6139
                     /locus_tag="OO515_003397"
     CDS             5909..6139
                     /locus_tag="OO515_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6577244.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            6151..6423
                     /locus_tag="OO515_003398"
     CDS             6151..6423
                     /locus_tag="OO515_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6577245.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            6650..6946
                     /gene="ghoS"
                     /locus_tag="OO515_003399"
     CDS             6650..6946
                     /gene="ghoS"
                     /locus_tag="OO515_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6577246.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            6974..7147
                     /gene="ghoT"
                     /locus_tag="OO515_003400"
     CDS             6974..7147
                     /gene="ghoT"
                     /locus_tag="OO515_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR6577247.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(7266..8783)
                     /gene="lysS"
                     /locus_tag="OO515_003401"
     CDS             complement(7266..8783)
                     /gene="lysS"
                     /locus_tag="OO515_003401"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6577248.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(9020..10477)
                     /gene="dtpC"
                     /locus_tag="OO515_003402"
     CDS             complement(9020..10477)
                     /gene="dtpC"
                     /locus_tag="OO515_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR6577249.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         2744 a         2514 c         2566 g         2707 t
ORIGIN      
        1 gcctttcgcc agcatgatca tgctgccgcc gtgggattgc agcagatcca cataggagtc
       61 catacgacct gcggtggttg ggccaagtga acctgatgga taaccggcag gagttttcgc
      121 cggacccgcg tagtagatcg ggtgatcttt gatgtactgc ggaagttctt taccggcgtc
      181 aatcagctct ttcagcttgg cgtgtgcaat atctcgaccc acaataatgg tgccagtgag
      241 cgacaaacga gtggaaaccg gatattgcga aagctgggcg aggatctctt tcatcgggcg
      301 gttaaggtcg accttcaccg cttcgccttc accggcctga cgcagttctt gtggaatgta
      361 ctgacctgga ttgtgttcca gtttttcgat ccagatacct tcgcggttga ttttcgcttt
      421 aatgttacgg tcagcggagc aggagacgcc catgcctacc gggcaggatg cgccgtgacg
      481 tggcagacgg ataacgcgaa tgtcgtgcgc gaagtattta ccgccaaact gcgcgccaag
      541 accgagtttc tgggcctctt ccagcagttc ctgttccagc tggacatcgc ggaacgcctg
      601 accatgttcg ttcccttccg tcggcagttc atcgtaatag tgagcgcttg ctaatttgac
      661 ggttttcagg ttggtttccg cagacgtacc gccaatcaca aacgcgatat ggtacggcgg
      721 gcaggctgca gtaccgaggg tacgcatttt ctcgacgagg aagtttttca gtttgccggg
      781 agtcagcagg gctttggttt cctggtagag atacgttttg ttggcagagc cgccgccttt
      841 cgcaacgcaa aggaatttgt actcatcgcc atctaccgcg tacaggtcga tttgcgcagg
      901 caggttagtg ccggtgttga cctctttgta catgtccagc gccgcgtttt gcgagtagcg
      961 caggttatct tcgatatagg tgttatagac gcctttcgac agcgcttctt catcaccgcc
     1021 accggtccac acgcgctggc cttttttacc gacgatgatc gcggtgccgg tatcctggca
     1081 ggtcggcagc acgcctttgg cggcgatttc ggagtttctt aagaattgca gtgccacgta
     1141 cttgtcgttt tcgctggctt ctggatcgtg aagaatagcc gcaacctgtt tctggtgtgc
     1201 cggacggagc atgaaagaag cgtcgtgaaa ggcttgctgc gccagcaggg tcagggcttc
     1261 tggttccact ttcaggatgg tttcgccgtc gaagtcggca acgctaacgt aatcggaagt
     1321 gagtagatag tattcggtat tgtctttccc catcgggaaa ggtgcctggt agataaaggg
     1381 tttgtttgac atagcttcca gcctgtaact ctgtatttgt tattcgaaaa tggcgtgccg
     1441 cgtagtcacg ggcggcacgc aaagtgcatt tataagaacc cgtacatcgc ggcgaagatc
     1501 cagccgaaga cgcacgatac gctcacacca atcaaccccg gcagaataaa gctgtggttg
     1561 atgacgaagc gaccgatgtg ggtggtgccg gaacggtcaa actgaatcgc tgccagatcg
     1621 ctcggataag tcggcaggat gtaataaccg tagcaagccg gtgctgaagc cacgatgtat
     1681 gccggatcaa cgccgatcgc cagcgcgacc ggaacaatcg ccgccagcgc cgcagcctga
     1741 gagtttacaa acttggaaac cagcagcaga acaatggcat aggcccacgg atactctttc
     1801 accatttcac ccagtacgcc ctgaatttca gacatatgcg caccgaacat ggtttctgcc
     1861 atccatgcga taccgtacac cgccacgatg gcgatcatac cggaacggaa gacttcgttt
     1921 tttgagatag acgcgggatt ggttttggtc aggataataa tcagcgcccc ggtcagcagc
     1981 ataaacatct gaataaccag taccatcgac agcggtttgc cgccgaagga tggacgcagg
     2041 tccgaatcag caccaagaag ggcgactaca gcgattgccc cgaggaaaat ccacattgcc
     2101 agccagttgc ttttcggcag ttttttgtcc agcagcgtcg cggtatcacc gtaaacatac
     2161 tcacggtttt ccggtacgga gatgaatttc tggaactctt cgtctttatc cagatcttta
     2221 ccacggaacc agctgaagat accgatcgcc aggataccga ttaacgtcga tggaatggtg
     2281 attgccagca ggtcgaggaa ctcaagatgg cgaccatcaa aggtgacatt acccagcatt
     2341 gcaaccagag acacgaccgc aaccgacacc ggactggcga tgatccccat ctgtgcaccg
     2401 atagaacttg ccgccatcgg acgttccgga cggatgttgt tcttaatggc gacgtcgtag
     2461 atgatcggca gaatggtgta aaccacatga cccgtaccgc aaagaatggt cagcgtacag
     2521 gtcacaaacg gcgcgacaat tgagacatat ttcgggttgc ggcgcagcag cttctcggca
     2581 atttgcagca tgacatcaag accgcccgaa gcttgcaagg tcgccgatgc cgccaccacc
     2641 gcaatgataa ccagcatgac atcaaccggt ggtttacctg gctgaaggtg gaagacgaag
     2701 accagaatga ccagaccgat accgcctaat aaacccagcg cgataccacc ctttctggca
     2761 ccataaaaca gacatatcag tattatgata agttggatag taaataacat gtgtgaaccc
     2821 tcgcgataat cctatttaaa tttttgctga atagatcaca gtcacgttct gttttgtatg
     2881 aactgttttc agagcctgac taaatatccg tttggtcgtt accggcttta gcaaatacct
     2941 cacagtgaat attggctgat taaagcagcg gcaaatttat gcactgtgat aagcggcttt
     3001 ttcagagaga aagactctct ggcgatgggt ttatagtttg tactgcgcag atgttaatgt
     3061 tttgtttgct gttatcttat tgatatttat cggtctattt tttgttctca gggcagatgt
     3121 cattaggttt atagattaat ctgatctacc catttgtggg taaaaataca cataatgcgg
     3181 gtgacataat agttaattaa cttttgttag cgttttgaaa ttaaaaacac cgttcacctg
     3241 aagagatatt aatttttagc gatgatgcag ggataattat atttgatctg gcacaagttt
     3301 tactgatgaa ggatgtaact tgtgccaggg gtattttgca ttacggtaat aattattact
     3361 tacagataac cagcgatccg ttattggcaa tattgtttca gtagtgagta gtgttctgcc
     3421 tgaatacggt aacggtaaac tggacgcccc gtgacgccat aatggatact ggtgaacaag
     3481 atgtggcagt tgaccagcca gatgaggtat ttacggcagg aaacacgcga aatgttaacc
     3541 tcgttggcta gctcgtcggt tgaaaattca tagtcctgat gcgcgtcaat ccactggcac
     3601 agtgtgcgta acgtctgcgg cgttaagcct tttggcaagc gacgaggatc ctgttcgttg
     3661 gagctgctgc cgtggattag ctgatcaagc tcggcctggt cataatactg atgtttttcc
     3721 agcgccattt tcttttgccg ccagtcggtg agcgcctctt caaagcggga agcctggaag
     3781 ggtttgatca ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg
     3841 gctgcggagg agatgacaat cacatcactt ttgcaacgcg cgttatgcag gacaggcagt
     3901 aaatcgagcc cgttctcttt ttgcatatag atatcgagca atatcaggtc gataggcgta
     3961 tcgctattga agataatctc tttggctttc tccagcgtcg aggctgttcc acagcattga
     4021 aagcctggga tttgtgctac gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg
     4081 ataattaata cattgatcat ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa
     4141 attgtgtgaa aatcccgggt tccgattcca cggcgatgct gccgccgaga ttttctacct
     4201 gttgtttgac aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt
     4261 caaaaatgtg atcgattttg tcgggtgcga tccccggtcc atcatcgtta acttcgcagt
     4321 gcagccagcc gtgacggtag tgcaatgtta cgctaatttc gccaccgggt tccggcccta
     4381 atgcttccag cgcgttttct atcagatttc ccaatgtggt aatcagcgtc gcgacctggt
     4441 cctcactgcc gctgtctggc agctggcttt cactgtttaa aatcagcgta tggcctaaat
     4501 cggtcgcgcg attaatcttg ctgattaaaa aaccagcgat aaccggagat ttgatcttac
     4561 ccagcagaga gccaatctct tcctgatagt tattggctgt ttttagaatg taatcttcca
     4621 actgcttata actcttcaga tgcaataatc cgagaatcac gtgcaattta ttcataaatt
     4681 cgtgggatcg ttcacgaagt gcgtcggcat agttgaccag accgtcgagt cgctgcatca
     4741 gtttacgtac ttcggttttg tccctgaagg ttgaaatggc accgatgata acgccattac
     4801 tgcgcaccgg aacggtgttg atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc
     4861 ggcgcggcgt accgtcgcgt aacacttccg agacatctac cacctgtgac catgagtggc
     4921 tgagcgtcga cagtttctca tcgtcctgcg acttacggta attcagcaat tcttgtgcgg
     4981 catcgttgat cagcgtgacc tcgccgcgat cgtccacggc aacgacgcct tctttgatag
     5041 actgcaacat ggcctggcgt tgctcaaaca gcgtggagat ttcgtagggt tccaggccga
     5101 aaaggatttt tttcagtacc ttaaccagaa tgcaggtgcc aatcagtccg accagcatgc
     5161 caaataatac cgaccagata atgctccagc gactgtcatt gatttgttgg gtcacgcggc
     5221 ttaactcaag gccgatcgcc accacgccaa tttgtttatg attttcatcg tagatggggg
     5281 taaatacgcg taaagcctgc gccagaaaac cgcgattgat agcgacattt tctttgccat
     5341 tcagcgcttt aaggatgtca tcacctttaa atggctgacc aatacgctgg gcttcaggat
     5401 gcgagtagcg aagactttgc atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc
     5461 gcacggcttc cgcgatggcc tggatgccgc tctcctgcgg ttttttctgc aagccctgac
     5521 ggatttccgg cgagttggcg agggtacgcg ccactgccag tgccttgttg gctagcccgt
     5581 ctcgcgtcat atcactgatt tgcgagaagt aaatcagatg caccaccaat agcaccgaga
     5641 acagtaccgc actgaccatt aagatcactg tggtactcaa tttcatcgga cgtttgcgta
     5701 acatgcggta gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt tcttaaacat
     5761 tatctctgat gaggtgggta attcaaaggg agtaagaatg attggctata taggggaaga
     5821 gattctggca acggaaactg ccagtgctgt atgaagattc cagggctatg cttatagcga
     5881 taatcatact gatgagagag ggaaagtcat ggatcaggcg ctactggacg agggttatcg
     5941 ctgttatacc ggcgaaaaga tcgatgtcta tttcaacact gcgatatgtc agcattctgg
     6001 caattgcgta cgtggcaacg gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc
     6061 ggatgaagtc gacgtcgcca ctgtggttaa agtgattgat acgtgcccga gcggcgcgct
     6121 gaaataccgt cataaataag cgagggtaaa atggaaatac gcgaaggcca caataaattt
     6181 tacattaatg acgaacaagg caagcaaatc gctgaaattg tctttgtgcc gactggagag
     6241 aatttagcga ttatcgaaca taccgatgtc gatgaaagcc tgaaagggca ggggattggt
     6301 aaacagctgg ttgcaaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca
     6361 ctatgcccat ttgcgaaaca cgaatttgat aaaacgcggg agtatgatga tattcgcagt
     6421 tgatgggaga gtacagagtc acgatatttt tcatgctctc cgcggagtga tgcaggagag
     6481 catctgaagg gtagggggat gcacaaagaa tgggcagaga gcgcgttttt ttgtcccaag
     6541 tcatcccctt tactgagcaa aaaaaagaat atctcctata tgagaatcat caatcggggt
     6601 tatcaagttt tgcgtcccca gagcattcaa tattgatagg agtcatatta tggaaggtaa
     6661 aaacaagttc aatacttatg ttgtttcttt tgattatcca tcatcttatt cctcagtgtt
     6721 cttaaggtta agatcattga tgtatgatat gaatttctct tctatcgtgg ctgatgaata
     6781 tgggatacca cgacaattga atgaaaattc cttcgcaata acgacatcgt tagccgcaag
     6841 tgaaatcgaa gatttaatca ggcttaaatg cttagactta ccggatattg attttgacct
     6901 caacattatg acagttgatg actatttccg tcagttttac aagtagccag gaaggataag
     6961 aagaacataa aaaatggcac tattctctaa aatattaatt ttttatgtga ttggtgtgaa
     7021 catatccttt gtcattatct ggtttatctc acatgagaaa acacatattc gtttacttag
     7081 tgcattcctg gtcggaataa cctggccaat gagtctgcct gtggcattac ttttttctct
     7141 cttttaggag ctgtagttga gtggacccgc tggttatcgt gaaacgaagc gtctctcctc
     7201 aaaactggat ttgcggaacg acgaacgaag cagtcgcctg actgcttcgt tcattaaagt
     7261 gaaatttatt tctgtgggcg catcgccggg aagagaataa cgtcgcgaat agtatgactg
     7321 tttgtaaaca gcataatcat tcggtcgata ccaataccca gaccagcggt tggcggcaga
     7381 ccatattcca gcgcagttac gtaatcttcg tcatagaaca tggcttcgtc gtcacctgca
     7441 gctttagcgt taacctgttc ctggaaacgt tcagcctgat cttctgcgtc gtttaattcg
     7501 ctaaaaccat taccgatttc acgaccaccg atgaagaatt caaaacggtc ggtgatttcc
     7561 gggttaacat cattacggcg cgccagcggt gacacttctg ccggatattc cgtaataaag
     7621 gttggctgaa tcagatgtgc ttctgccact tcatcaaaga tctccgtgat aatacgtccc
     7681 agcccccagc ttttctctac cgtaattccg atagattcag ctaatgcttt agcagcatca
     7741 aaattatcca ggtcggccat atcggtttct ggacgatatt ttttgattgc ttcgcgcatg
     7801 gtgagttttt caaacggttt gccgaaatca aacacatgct cgccataagt gactttagtg
     7861 gtacccagaa cctcttgtgc cagggtgcgg aacagtgact ctgtcagttc aatcaaatcg
     7921 tggtaatccg catacgccat gtagagttcc atcattgtga actcaggatt atggcgaaca
     7981 gaaatacctt cattacggaa gttacggttg atttcgaata cccgttcaaa accgcctaca
     8041 accagacgtt tcagatacag ctccggcgcg atacgcagat acatatctaa atccagagca
     8101 ttatgatggg taataaacgg gcgagcagat gccccacctg gaattacctg catcatcggg
     8161 gtttctactt ccataaagcc gcgcgcgacc atgaactgac ggatagcggc cagaattttt
     8221 gaacggacaa caaacgtttg acgggattta tcgttagcga tgaggtccag ataacgttga
     8281 cgataacgga cttcctgatc ctgcagacca tggaatttat ctggtaaagg acgtagtgct
     8341 ttagtcagca ggcgcaactc agtacagtga atggaaagct cacccgtttg cgtcttaaac
     8401 agcgtaccgc gggcaccgat aatgtcaccc agatcccatt ttttaaactg atcgttataa
     8461 acaccttctg gcaggctatc tcttgcaacg tacagttgaa tacggccacc gacatcctgc
     8521 aacgttacaa aggaggcttt ccccatgata cgacgggtca tcattcggcc agcaaccgag
     8581 acttcaatgt ttaaggattc cagttcctgg ttattcttcg catcaaactc ttcgtgcaac
     8641 tggtcagagg tatggtcgcg gcgaaaatca ttgggaaacg ccacaccttg ctgacgtagt
     8701 gccgccagtt tttcgcggcg atttctcagt tcatcgttaa aatcaatagc ctcattggct
     8761 ccccgtgttt cttgttcaga cattttggtt cctctaaatc cagctttcaa tttagcgtag
     8821 ataaagagac agatcggtac actgtattat ctgcctcgac tattaataac tcagtttatc
     8881 ttatcgaaac taacgcttac ctcaagttga tatcactaat aaataaccat caaaatggtt
     8941 taaatgactt attaatctcg atttactagt gtgattaact gaaagttaat ttattcataa
     9001 ccaattgttt ttacgacagt taatcgttgc tctcctgtat catattcgtc ggcgtgctaa
     9061 acagaaaacg ggtagcaaag gccaatacga cgatgatcgc gacacaagcc aacgtccact
     9121 ctcccatctg agaaaagaat cgctggtaag ctgcaattac cataccgcta atttgcgact
     9181 ctgtcgtctg ctgtgccacg acgcctgcca gccagttggc gaccgcgcct gttgccagca
     9241 tataaatacc ggttaatacg ccagacattt tcagacgcgt gatttgcgca atcgccaccg
     9301 ggtcaataaa gagttctgca aagcccatta gcgccaaccc ggatatcatc acgcccattg
     9361 acgcttgacc gtcagccgct gcatgtcggg catcaaatgc caacaacata aagccacaag
     9421 ccatcagcag taagccaaag gcaaacttca gccagacgcg caatgttgag ttgccgcggc
     9481 tttctggcga cgccagccag gccagtacaa ccccagcgag catcactgca attgcattca
     9541 ccgactggaa tagtgctgta ggtacttcaa tattgaatgc ctgacgattc acaaagcgat
     9601 cgataaacaa gctgatggta ctgccgccct gttgtgccag tacccagaac aacgtcccga
     9661 caaacatcaa caatacaatt tgccaaagag cacggcggtg ttcggggaat ttgatcatca
     9721 tgcgggcaat gatttgtgcg gcaatgaggc aaacgatcgc cagcaaatat ccggaccagt
     9781 cgttctccag cagcagggta aaaaatactg gggctaaaca gagcatcacc actaaccagc
     9841 tccatactgg taaggcaaat ttgacgctag tgagcgcttt tttatccata ctacgcgtgg
     9901 actggaaatg acgatgaccg cttaagaaaa tcaacaaacc gataaacatg ccgccacccg
     9961 caagggcaaa gccaacatgc catccatacc actgagcagc caggccgcag gcgattgggg
    10021 cggcgataga accgatattg cccgcagcat acagcagcga aaaaccgcca tcacgtcgat
    10081 gatcgttctc gtcgtagagc tcgccaagca aacagctgat gtttgatttg aataaaccgt
    10141 agccacaaat aatgattgcc agcgccagat acaggctaaa ggttgaattt gtatcaatgc
    10201 ccagcaccac atggccaagg gtcattaaca gcgcgccggc aatcactgca gtgcggttgc
    10261 cgagcaggcg gtcggcaagc cagccgccga gaataggggt aacgtaaacc agagaagcat
    10321 atgcgctgaa caggctgatg gcatggttat catcaaaacc aagctgatgg gtgagataga
    10381 gaatgagtaa ggcacgcatg ccgtaaaaac tgaagtactc ccagatttgg atcgccacga
    10441 tatagtatat cgcgcgcggc tgtgagggtg ttttcatgtg ttctccttat gagcaaaaaa
    10501 gggaagtggc aagccacttc ccttgtacga g
//
LOCUS       DAJTXQ010000136        10430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000136 DAJTXQ010000000
VERSION     DAJTXQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.136"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OO515_003403"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OO515_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6577250.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO515_003404"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO515_003404"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6577251.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OO515_003405"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OO515_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6577252.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OO515_003406"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OO515_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6577253.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OO515_003407"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OO515_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OO515_003408"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OO515_003408"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6577254.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OO515_003409"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OO515_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6577255.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO515_003410"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO515_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6577256.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OO515_003411"
     CDS             complement(9457..10149)
                     /locus_tag="OO515_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6577257.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2516 c         2755 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAJTXQ010000137        10363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000137 DAJTXQ010000000
VERSION     DAJTXQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.137"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..154
                     /locus_tag="OO515_003412"
     CDS             <1..154
                     /locus_tag="OO515_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6577258.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OO515_003413"
     CDS             258..1172
                     /locus_tag="OO515_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6577259.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OO515_003414"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OO515_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6577260.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OO515_003415"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OO515_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6577261.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OO515_003416"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OO515_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6577262.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OO515_003417"
     CDS             5467..7149
                     /locus_tag="OO515_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6577263.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OO515_003418"
     CDS             7201..8358
                     /locus_tag="OO515_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6577264.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OO515_003419"
     CDS             complement(8324..8446)
                     /locus_tag="OO515_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577265.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OO515_003420"
     CDS             8655..10340
                     /locus_tag="OO515_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6577266.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2208 c         2214 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg tac
//
LOCUS       DAJTXQ010000138        10210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000138 DAJTXQ010000000
VERSION     DAJTXQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.138"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="OO515_003421"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="OO515_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6577267.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="OO515_003422"
     CDS             complement(1127..1213)
                     /locus_tag="OO515_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6577268.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="OO515_003423"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="OO515_003423"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6577269.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="OO515_003424"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="OO515_003424"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6577270.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="OO515_003425"
     CDS             3562..3606
                     /locus_tag="OO515_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577271.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="OO515_003426"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="OO515_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6577272.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="OO515_003427"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="OO515_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6577273.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="OO515_003428"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="OO515_003428"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6577274.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="OO515_003429"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="OO515_003429"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6577275.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="OO515_003430"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="OO515_003430"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6577276.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..>10210
                     /gene="leuD"
                     /locus_tag="OO515_003431"
     CDS             9620..>10210
                     /gene="leuD"
                     /locus_tag="OO515_003431"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6577277.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQP"
BASE COUNT         2673 a         2682 c         2500 g         2355 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct
//
LOCUS       DAJTXQ010000139        10016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000139 DAJTXQ010000000
VERSION     DAJTXQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.139"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            698..2329
                     /gene="oppA"
                     /locus_tag="OO515_003432"
     CDS             698..2329
                     /gene="oppA"
                     /locus_tag="OO515_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6577278.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2415..3335
                     /gene="oppB"
                     /locus_tag="OO515_003433"
     CDS             2415..3335
                     /gene="oppB"
                     /locus_tag="OO515_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6577279.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3350..4258
                     /gene="oppC"
                     /locus_tag="OO515_003434"
     CDS             3350..4258
                     /gene="oppC"
                     /locus_tag="OO515_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6577280.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4270..5283
                     /gene="oppD"
                     /locus_tag="OO515_003435"
     CDS             4270..5283
                     /gene="oppD"
                     /locus_tag="OO515_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6577281.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5280..6284
                     /gene="oppF"
                     /locus_tag="OO515_003436"
     CDS             5280..6284
                     /gene="oppF"
                     /locus_tag="OO515_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6577282.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6337..6666)
                     /locus_tag="OO515_003437"
     CDS             complement(6337..6666)
                     /locus_tag="OO515_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6577283.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6701..8161)
                     /gene="cls"
                     /locus_tag="OO515_003438"
     CDS             complement(6701..8161)
                     /gene="cls"
                     /locus_tag="OO515_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6577284.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8304..8477
                     /locus_tag="OO515_003439"
     CDS             8304..8477
                     /locus_tag="OO515_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6577285.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8532..9785)
                     /gene="kch"
                     /locus_tag="OO515_003440"
     CDS             complement(8532..9785)
                     /gene="kch"
                     /locus_tag="OO515_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6577286.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2639 a         2321 c         2462 g         2594 t
ORIGIN      
        1 tcgtcattat ttttactttg ttcccgcgca gttatcaaaa gcaaaaggaa taggtaaaaa
       61 tattcttctc aaattacagt tagttataag gatttcctta actgcttctc ctcaccatca
      121 tgttattttc gccacatcat aatcctgggc ttgctgaaga ataattgaaa tgatattagt
      181 aattccactg cctttggtag aggaaagtgc taaataataa tcaattgtta aattattgtg
      241 catttcacta ctggaactgt aatcagaaaa gatagacatg cttagccaat ctctatttga
      301 ttgaattgaa agatgtttgt taaggcatgg atgcaagcta tagattctga tacggtcaat
      361 aaaagagaat tgcttaacaa ttttgcaaaa tgtattggcg agtaagaacc gcatttggta
      421 ctttccgggc aaccgccaga cgattcttta ttggtaatga gaataattaa caattaaaga
      481 gcgtcgcgaa agaataatgt gtctcgacag gggagacaca gtacgaatcg acataaggtg
      541 atcgtctgaa tcaccagaat aaataaagtc ggtgatagta atacgtaacg ataaagtaac
      601 ctgacagcag aaagtctccg agcctgtgca gggtcccaat ccgggattac acatgctggt
      661 taataccagt aattataatg agggagtcca aaaaacaatg accaacatca ccaagagaag
      721 tttagtagca gctggcgttc tggctgcgct aatggcaggg aatgtcgcgc tggcagctga
      781 tgtacccgca ggcgtcacac tggcggaaaa acaaacactg gtacgtaaca atggttcaga
      841 agttcagtca ttagatccgc acaaaattga aggtgttccg gagtctaata tcagccgaga
      901 cctgtttgaa ggcttactgg tcagcgatct tgacggtcat ccagcacctg gcgtcgctga
      961 atcctgggat aataaagacg cgaaagtctg gaccttccat ttgcgtaaag atgcgaaatg
     1021 gtctgatagc acgccagtca cagcacaaga ctttgtgtat agctggcaac gttctgttga
     1081 tccgaacact gcttctccgt atgccagtta tctgcaatat gggcatatcg ccggtattga
     1141 tgaaattctt gaagggaaaa aaccgattac cgatctcggc gtgaaagcta ttgatgatca
     1201 cacattagaa gtcaccttaa gtgaacccgt tccgtacttc tataaattac ttgttcaccc
     1261 atcaacttca ccggtgccaa aagccgctat cgagaaattc ggcgaaaaat ggacccagcc
     1321 tggtaatatc gtcaccaacg gtgcctatac cttaaaagat tgggtcgtaa acgaacgaat
     1381 cgttcttgaa cgcagcccga cctactggaa caacgcgaaa accgttatta accaggtaac
     1441 ctatttgcct attgcttctg aagttaccga tgtcaaccgc taccgtagtg gtgaaatcga
     1501 catgacgtat aacaacatgc cgatcgaatt gttccagaag ctgaaaaaag agatcccgga
     1561 cgaagttcac gttgatccat acctgtgcac ttactattac gaaattaaca accagaaacc
     1621 gccattcaac gatgtgcgtg tgcgtaccgc actgaaacta ggtatggacc gcgatatcat
     1681 tgttaataaa gtgaaagcgc agggcgacat gcccgcctat ggttacactc caccgtatac
     1741 tgatggcgca aaattgactc agccagaatg gtttggctgg agccaggaaa aacgtaacga
     1801 agaagcgaaa aaactgctgg ctgaagcggg ttataccgca gacaaaccgt tgaccatcaa
     1861 cctgctgtat aacacctctg atctgcataa aaagctggcg attgctgcct cttcattgtg
     1921 gaagaaaaac attggtgtaa acgtcaaact ggttaaccag gagtggaaaa cgttcctcga
     1981 cacccgtcac cagggcactt ttgatgtggc ccgtgcaggc tggtgtgcag actacaacga
     2041 accaacttcc ttcctgaaca ccatgctgtc gaacagctcg atgaataccg cgcattataa
     2101 gagcccggcc tttgacagca ttatggcgga aacgctgaaa gtgactgacg aggcgcagcg
     2161 cacagctctg tacactaaag cagaacaaca gctggataag gattcggcca ttgttcctgt
     2221 ttattactac gtgaatgcgc gtctggtgaa accgtgggtt ggtggctata ccggcaaaga
     2281 tccgctggat aatacctaca cccggaatat gtacattgtg aagcactaat ggcaatacgt
     2341 ggggcaggag tgtcctgctc cacggtgtct gatttttatc gcattacaga aggcacaggc
     2401 cagaaggtag ggcaatgtta aaatttattc tacgtcgctg tctggaagcg attccgacgc
     2461 tatttattct tattactatt tcgttcttta tgatgcgcct cgcgccggga agccctttta
     2521 ccggcgaacg tactttaccg ccggaagtga tggccaatat cgaagcgaaa tatcatctta
     2581 atgatccgat catgacacag tatttcagct acctgaaaca actggcgcac ggtgatttcg
     2641 gtccatcgtt taaatataaa gattattcgg tcaatgacct ggttgcatcc agttttcccg
     2701 tttctgccaa actgggagcc gccgcatttt tccttgcggt aatactgggt gttagtgctg
     2761 gcgttattgt cgcattaaaa caaaacacca aatgggacta taccgtgatg gggctggcaa
     2821 tgaccggggt tgttatcccc agttttgtgg ttgcgccatt attagtcatg atatttgcga
     2881 tcattttgca ttggctgccg ggcggtggct ggaatggtgg ggcgcttaaa ttcatgatat
     2941 tgccgatggt ggcgttgtca ctcgcttata tcgccagtat tgcgcgtatt acccgaggct
     3001 ctatgattga agtattacac tccaacttta ttcgtactgc ccgggcgaaa gggttaccta
     3061 tgcggcggat cattttacgc cacgcattaa aacctgctct gttacccgtg ctctcttata
     3121 tgggccccgc atttgtcggc attattaccg gttctatggt tatcgaaacc atttatggtt
     3181 tgccggggat tggacaactg ttcgttaatg gcgcattgaa ccgtgactat tccttagtgt
     3241 taagcctgac catcctggtt ggtgctttaa ccattttgtt taatgccatt gtcgatgtgc
     3301 tatatgcggt tatcgacccg aaaatccgtt actgatactg gagctcgcga tgatgttaag
     3361 taagaaaaac agcgagacgc tggaaaattt cagtgaaaag ctggaggtcg aagggcgcag
     3421 cttgtggcag gacgcacgtc gacgttttat gcataaccgt gcggcggttg ccagtctgat
     3481 agtgctggtg ctgatcgcgt tatttgtaat cctggcaccg atgctttcgc agtttgccta
     3541 tgacgatact gactgggcga tgatgtccag cgccccggat atggagtccg gtcactactt
     3601 tggtactgac tcatccggtc gcgacctgct tgtgcgcgtt gcgattggcg ggcgtatctc
     3661 actcatggtc ggtgttgctg cggcactggt ggcagtggtc gtggggacac tttacggttc
     3721 gctttccggt tatctgggcg gtaaagtgga ttcggtaatg atgcgtctgc tggaaatcct
     3781 caactccttc ccattcatgt tcttcgtcat tttgcttgtg acctttttcg gtcaaaacat
     3841 cctgctgatt ttcgtggcga ttggcatggt ttcctggctg gatatggctc gtattgtgcg
     3901 tggacaaacc ctgagtctga agcgcaaaga gtttattgag gcggcacaag ttggcggtgt
     3961 atcgacatcg ggcattgtta ttcgccacat tgtgccgaat gtactcggtg tggtggtggt
     4021 ctacgcatca cttctggtgc ccagcatgat cctctttgaa tctttcctta gcttcctggg
     4081 gttgggtacg caagagccgt taagcagctg gggtgcattg ctgagtgatg gcgcgaactc
     4141 gatggaagtc tctccatggt tattgttgtt cccagcggga ttcctcgtgg tgacgctgtt
     4201 ttgtttcaac tttatcggcg atggcttgcg tgatgctctc gacccgaaag atcgttaagg
     4261 agtgcagcca tgagcgtaat tgaaactgca actgtgccgc tcgcacaaca acaggctgac
     4321 gtactgctga acgtgaaaga tttgcgtgtc acctttagta ccccggacgg cgatgtcacg
     4381 gcggtcaatg atttgaattt ttctctacgt gccggagaaa cgctgggcat tgtaggtgag
     4441 tctggttcgg gtaaatcgca aactgcattt gcgttgatgg gcctgttggc tgccaacgga
     4501 cgtattggcg gatcggcaac cttcaatggg cgtgaaatcc tcaatttgcc agagcgtgaa
     4561 ctcaataaac tgcgcgctga acaaatctca atgatttttc aggacccaat gacttcgttg
     4621 aatccctata tgcgcgtcgg tgagcagttg atggaagtgc tgatgctgca taagaacatg
     4681 agcaaagctg aggcgtttga agagtcggtg cggatgctcg atgcggtaaa aatgccggaa
     4741 gcacgtaaac gcatgaaaat gtacccgcac gaattttctg gtggcatgcg tcagcgagtt
     4801 atgattgcga tggcattgct atgtcgacct aagctgctga ttgcggatga acccactact
     4861 gcgctggacg tcaccgtaca ggcgcagatc atgacgctat taaatgaact gaagcgggaa
     4921 tttaataccg ccatcattat gattacccac gatcttggcg tggtggcggg gatctgcgac
     4981 aaagtgctgg taatgtacgc cgggcgcacg atggaatatg gcaacgcgcg cgatgtcttt
     5041 tatcaacccg ttcatcctta ttctatcggt ttgctcaacg cggtgccgcg tctcgatgcg
     5101 gaaggtgaaa caatgttgac catccctggt aatccgccaa acctgctgcg attaccgaaa
     5161 ggttgcccgt tccagccacg ttgtccgcat gcgatggaaa tttgtagtag cgcaccgccg
     5221 ctggaagagt ttacgcctgg ccgtctgcgt gcttgcttta aaccggtgga ggaactgtta
     5281 tgaatgctgt aaccgaagga agaaaagtcc tcctcgaaat cgccgatctg aaagtgcact
     5341 ttgaaatcaa agatggcaaa cagtggttct ggcaaccgcc gaaaactctc aaagccgtcg
     5401 atggtgtcac gcttcgcctg tatgaagggg aaacattagg tgtggtaggg gaatcgggat
     5461 gcggtaagtc cacctttgct cgcgccatca ttggcctggt taaagcgacc ggcggtcatg
     5521 ttgcctggtt aggtaaagag ttgctgggca tgaagcccga tgaatggcgt gccgttcgca
     5581 gtgatattca gatgattttc caggatccgt tggcatcgct aaacccgcgt atgaccatcg
     5641 gcgagatcat cgctgaacca ctgcgtactt atcatccgaa aatgtcacgc caggaagttc
     5701 gcgagcgcgt gaaggcgatg atgctgaaag tcgggttatt gcctaacctg attaaccgct
     5761 atccgcatga gttctccggt gggcagtgcc agcgtatcgg gattgctcgt gctcttattc
     5821 ttgaaccgaa gctgattatc tgcgatgagc cgatttcggc gctggacgtg tcaattcagg
     5881 cgcaggtggt caacctgctc cagcagctgc aacgtgagat gggattgtca ttaattttta
     5941 tcgctcatga cctggccgtg gtaaaacaca tttccgatcg tgtgttggtg atgtatctcg
     6001 gccatgcggt agaactgggg acctatgatg aggtctacca caatccacta catccttaca
     6061 ccagggcatt gatgtcggca gtccccatac ctgatccgga tctggagaag aacaaaacca
     6121 tccagttact ggaaggggaa ttaccgtcgc cgatcaaccc gccttccggt tgtgttttcc
     6181 gtacctgttg cccgattgcc ggtccggagt gcgccaaaac acgtcctgtg ctggaaggga
     6241 gtttcagaca cgccgtttct tgcctgaaag tcgatccgct ttaaacacaa taagggctga
     6301 cagttgtcag ccctttttca cgccgaaagc gattatttat tcccgccaga tgatatggca
     6361 aagtttgtga tctttttcgc ggcataacag aatgcgggcg aaaatatcgt tgatttcacc
     6421 atcctcactg tccgccagac caatcaccac ttcggcaaaa aagtccgggt tcaaatcgaa
     6481 atcaacatgt tcctgccagt cttccgccgg atcgaataac tccgcgccgc cgcgctcttc
     6541 aaactgaaga ttgaacagca gaacatcagc tgggtcgaga ttgtcggcag ccagttcgag
     6601 aaaaatatcg taggcctgct caagcgtttc atcttcagtc aggcgattgt ttagatccat
     6661 atccatagtc actacctgtt taacctctgt tggcgacgtt ttacagcaac gggctgaaga
     6721 agtaaaacag tcgctcggcg acacgttgcc ataatggacg ttttagccat aaacgggcat
     6781 cgagcagacg tgaacgcgaa atataatcgt cctgaacggc ggcaaggtct gcaccaaaac
     6841 ctttatcgtc gattgccagg gtaatctcga aattcagcca cagactacgc atatcaaggt
     6901 taacggtgcc aaccagactt agttcgccat cgaccagcac gctcttggta tgcagtaacc
     6961 cgccttcaaa ctgataaatt ttaaccccag cagcaagcag ttccgtaaag aatgcgcgac
     7021 tggcccagcc gaccagcatc gagtcatttt ttcgcggaag gataatactg acatccaccc
     7081 cgcgctgcgc cgccgtgcaa atcgcatgaa gtaaatcatc gcttggcaca aagtagggcg
     7141 ttgtcatgat caaatattca cgcgccgaat aagccgcagt caataatgcc tggtgaatga
     7201 gatcttccgg aaagccgggg ccagaagcaa ttgtgtgaat ggtgtgaccg ctggcctgtt
     7261 caaacggcat aatattgaca tctggtggtg gcggcagaat acgttttccg gtttcaatct
     7321 cccagtcgca ggaataaata atccccatcg cggtggcgat ggggccttcc atacgcgcca
     7381 tcagatcaat ccattgccct acgcccgcat cttgtttgaa gtagcgagga tcgaccatat
     7441 tcatgctgcc ggtgtacgcg atgtaattat cgatcatgat catcttgcga tgttggcgca
     7501 ggtccatacg gcgtaaaaac acacgcatca gattgacctt taaggcttcg accacttcaa
     7561 taccggcatt acgcattagc tcgggccacg ggctgcggaa aaaagccaca ctcccggcgg
     7621 agtcgagcat caatcggcaa tgaataccac gccgtgcagc cgccattaat gattcagcca
     7681 cctggtccgc catgccgccg ggctgccaga tataaaacac catctcaata ttatggcgcg
     7741 cgagctggat gtcgcggatt aacgcctgca tcacatcatc tgactcggtc atcagttgta
     7801 gctgattccc tttgacccca gcgatcccct gacgacgctc gcaaagcttg aataatggcg
     7861 cagcgacact gctattttct tcggcgaaga tatgcttaca ggctttaagg tcgttaagcc
     7921 attttgcggt ggaaggccac atcgctctgg cgcgctcagc gcggcgtttg cctaaatgga
     7981 gctcgccaac ggcaagatag gcaataattc cgactaacgg cagaatgtaa ataatcaaca
     8041 gccaggccat cgcggaggga actgcgcgtc gtttcattag aatgcgtaaa gttacgcctg
     8101 caatgagcaa ccagtatccc agaatggcca accaactcac caacgtataa acggttgtca
     8161 tagattagaa atccttttga aagcgcattg ttatgagttt acgcatatgg attcatctgg
     8221 caaataaaaa cgcgggaaaa gcactggtca gcgtcggggg tgggggtata atgaccattc
     8281 tgttattgca tagagtagtt aacatgaagc ggagtagaac ggaagtgggg cgctggcgca
     8341 tgcagcgtca ggctagccga cgtaaatcgc gttggcttga ggggcaatcg cgccgaaata
     8401 tgcgtatcca cagcatcagg aagtgcattc taaacaaaca gcgtaactcg ttattgtttg
     8461 cgatctacaa tatctaaata tactgggcac cattgcggtg cccaataagt ttactctttc
     8521 gtctttaaac tctatttttg cactgattct ttactgtcag ctttcgtttc ttgttcatcg
     8581 ttatcgctga aaatgccatg accggagttc aacaacattg aaacgagcat atcattatta
     8641 atctcttcac catttaaaac tcgcgccaga atttcgctgc caaacagttg cggtgagaga
     8701 atgatatccg gatgtaccat cttaatctta ttcaggtttt tgctatcact gacggcgaga
     8761 actgttttga catcactgct catatctttt gccgagagta caacaaacgc gttatctgca
     8821 tcgttatcac tcagcgccag aatggcccgg caacgatcga ttcccgcttt ctttaatact
     8881 gaactgtcat tactgtcacc ggggataaca tcagcgttat cgcctaagcg ttgctcaagt
     8941 tgcttgatat catcttcagg caagttgctg ataaccgtta cgttttgtcc gcgttgattc
     9001 agttgcagga tcgtattgat ggcgagaatc gaatgtccgc aaacaataaa atgatcttta
     9061 cgatgcattg tatgattgtt tccttttaca agtttgttga atcccccgcg gataagcgga
     9121 ccaaaaattg aggtcataga tgtggcaaaa acggtaatgc cggaaataat gaccgaaata
     9181 gtgaacaatc gtgctgattc agaaacaggg acaatatcgc catagccgac ggttgacatg
     9241 gtttctatcg aaaaatagaa cgcggtcatc aaactttcta ttcgcggatt aaaaccttcg
     9301 cttaaataaa gcgcaccgta ggttgagtaa aacagtaatg tcgtgaaact aataaatgca
     9361 aaaattgtcc cggctgcggc gctactgtgg gagaagtctt tgcgcagtat cagcaaaaac
     9421 accagcgtaa aaatgcaaaa tccaatacta aatttcagcc agggataaaa atgcagggtg
     9481 taaattagcg ctatcaacaa caaaatgata ctgattgccc atgcgagctt tgcccggaac
     9541 agtaagccaa tggagttcag gacaagaaag acgccgagca taaatagtgg ggcgtttgcc
     9601 agcaaggaga gatccaactc tgaaaatgct ttgatatgga aaatatcaag caaattgacc
     9661 gacataccat agattgtttt aaaaataagc aatccattta aaaagacggc cagcgcgaga
     9721 atgtcgtgcc gaagggtcac ccataaattt gttgctgttt gtttgaatgt agcccagtga
     9781 ctcactgata cctctgtaac cacaattttg tttaattatc ttagcgtgca cattttctga
     9841 acatacatgc agcgcgtgaa tgtgttaaac aatagtagat gactttatcc gcagtaatca
     9901 aaggatttga gcgagcaact gtacctcagg aaaggtcatt tttcctgaat atgctcacat
     9961 catataaaga aatacagata aagttattat ctgcttgtgg tagtgaatgc actgac
//
LOCUS       DAJTXQ010000140         9960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000140 DAJTXQ010000000
VERSION     DAJTXQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.140"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..1135)
                     /locus_tag="OO515_003441"
                     /pseudo
     CDS             complement(1..1135)
                     /locus_tag="OO515_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1135..1317)
                     /locus_tag="OO515_003442"
     CDS             complement(1135..1317)
                     /locus_tag="OO515_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577287.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1329..3062)
                     /locus_tag="OO515_003443"
     CDS             complement(1329..3062)
                     /locus_tag="OO515_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6577288.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3059..3553)
                     /locus_tag="OO515_003444"
     CDS             complement(3059..3553)
                     /locus_tag="OO515_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6577289.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3675..4025)
                     /locus_tag="OO515_003445"
     CDS             complement(3675..4025)
                     /locus_tag="OO515_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6577290.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4068..4532)
                     /locus_tag="OO515_003446"
     CDS             complement(4068..4532)
                     /locus_tag="OO515_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6577291.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="OO515_003447"
     CDS             complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="OO515_003447"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR6577292.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5026..5241)
                     /locus_tag="OO515_003448"
     CDS             complement(5026..5241)
                     /locus_tag="OO515_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6577293.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(5922..5996)
                     /locus_tag="OO515_003449"
     tRNA            complement(5922..5996)
                     /locus_tag="OO515_003449"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5961..5963),aa:Gly,seq:tcc)
     gene            complement(6000..6075)
                     /locus_tag="OO515_003450"
     tRNA            complement(6000..6075)
                     /locus_tag="OO515_003450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6040..6042),aa:Thr,seq:tgt)
     gene            complement(6078..6154)
                     /locus_tag="OO515_003451"
     tRNA            complement(6078..6154)
                     /locus_tag="OO515_003451"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6118..6120),aa:Arg,seq:tct)
     gene            complement(6156..6232)
                     /locus_tag="OO515_003452"
     tRNA            complement(6156..6232)
                     /locus_tag="OO515_003452"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6196..6198),aa:Ile,seq:cat)
     gene            complement(6422..6985)
                     /locus_tag="OO515_003453"
     CDS             complement(6422..6985)
                     /locus_tag="OO515_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6577294.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(7089..7307)
                     /locus_tag="OO515_003454"
     CDS             complement(7089..7307)
                     /locus_tag="OO515_003454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577295.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(7466..7852)
                     /locus_tag="OO515_003455"
     CDS             complement(7466..7852)
                     /locus_tag="OO515_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6577296.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(7842..8213)
                     /locus_tag="OO515_003456"
     CDS             complement(7842..8213)
                     /locus_tag="OO515_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6577297.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(8213..8470)
                     /locus_tag="OO515_003457"
     CDS             complement(8213..8470)
                     /locus_tag="OO515_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577298.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(8467..9858)
                     /locus_tag="OO515_003458"
     CDS             complement(8467..9858)
                     /locus_tag="OO515_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6577299.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(9855..>9960)
                     /locus_tag="OO515_003459"
     CDS             complement(9855..>9960)
                     /locus_tag="OO515_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6577300.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT         2309 a         2826 c         2351 g         2474 t
ORIGIN      
        1 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tggaagagta aattccccag
       61 ttgatcccgg tggcgtaggc ttcaaaacgg gacttcatat ccccgcgcag taacgccccg
      121 gcgttaaatt tggcgtaata aacgccctgc ttactttttc gtaccagtcc ggtgttgatc
      181 cgctgttcga tgcgggtcag atacggcacc agtgaatagt tgataaatcc cagccccagc
      241 tcttcgatat tgttgaaggt ggcgcgatcg gtgttctgca ccatgtgcaa cggcacccgg
      301 aacagacgac agatttcttc cagctgaaac ttgcgggttt ccaggaactg actgtcctcg
      361 gcgttcagcg ccatcgactt ccagtccagc cccatttcca gaatcatcgg gcggtgagca
      421 ttgccaagcc ccgtgtgaca ctcctcaaaa tctttcttca gacgctcgta agcatccggc
      481 gtgagctttt gttccgtacg caacacaccg gatgtcaccg caccattact aaacaacctg
      541 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cacgcgcata cgcaatggga
      601 ttcagcccga caagtccatc cagcgtcagg gtgcgcacat gccagatttc attctgggtc
      661 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
      721 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccaccacttc tcccagtgcc
      781 tttaccttgt aggcgtaaaa attcccccgt agacacaggc agacaataac cagctcccag
      841 aattcctgcg gtgtcatgta gccattaggt tttgccgaaa ccaacttatg cagtcgttca
      901 tccaccgccc gtgttttcag ggtaccgctg attttgtaga gactgcaggg cagcatacca
      961 acagactctg ccagcaccct gacgcaagaa tagaccgccg tcaggcgcat ggcccgctgg
     1021 ctactgatcc gctttccggt ataggtgtcg tatgacagcc cgatagcatc agcagttctg
     1081 ctggcgtggt caccggcaca tcgctttttc gttgaaataa tcccgaaaag aacactattt
     1141 acctccgccg acagactgcc gtgtacggtc gagatatcgc gccaccagcc acgaccagaa
     1201 caggcacagc gccccggcaa caacaaaacc cgccggggga taaatcagcc aggcaccata
     1261 cgccagcaaa agcgcaccca gcacgcccac cagaggcgcg agaatcagca tgatcataat
     1321 tacctcagtt aaagcgagcg gatcccgtag gactcaatgt ggtcagacag cgtgtcttct
     1381 ttctcgtaca gcatggctct gccaaccgcc ataatcagcg caactgcacc atcgattttg
     1441 ttttccgcct gctctttgac gggtttcacc acatcatcgt tacccggcat gtttttgccg
     1501 accacgttgc cgatacacca ggtcatgatg ggattgccgt catgatgaaa acgtcccgat
     1561 tcaatcgctg cttccagctc tttcatcgga tcggacatat tggtgaagtt ctggacgata
     1621 gtgacgggat tcaggtcttc atcagcaagg tcatgtgaca acccggtcgc cccgaagggg
     1681 tcgatgggtg actcactgac cgggctgatt ttgttcgccg ctttggcctc ctcgaggatg
     1741 tagcgataat ccacctccgc accatcggta acggtcagaa cgcccatttc cacccatttc
     1801 tgaaagcgtt cggctgtccg tcgatcttca tttttctcga cgctgtacac cgtgtcatac
     1861 ggtacccaga aacgcggggc cacactgtag taatgcgttt taccgtcaat ctcgcgggta
     1921 taaagtcgcg ccatgctgtt catatccagc ttacgcgcca ggtcaaaggc cagaatgcac
     1981 ggctgcccct cgaactgctc aagggtcagt gatttatcct cgcagctctg ccagctcacc
     2041 aagttgaaat acgccgaacg cgccgacacc cagatattga ggtgttttgt tttaaagacg
     2101 tttgccagac gggcgttatt tttcgcacgc tgctgctgac ttaacaaaaa ttcgcgataa
     2161 accgacacgc caatatttgg attggctttt tccagcacct gcgggccggt ccagtcgtca
     2221 ccttcatcaa cggtatagat gatcccgaac agttcatcgt taggcaccga gccgttgagc
     2281 atctcgatga cttcccgccg cttgtcgtag cacggcccct caatgttgta cccggcggtg
     2341 gtgatggccc acatcagtgg ctgacgtcgc gcccccatcc cggtaagcat tgtggtataa
     2401 agcgcatcgg tggcatgctc gtgatattca tcaaccacgg cacagtgggg tgatgaacca
     2461 tcacctgggt tgccgatcag cggttcaaac cgcgcgccat cctccggacg gttcatgttt
     2521 gaggcgttaa cctcaatccc gaacgcttcc gtcagcatgg gtgtgcgttt acacatcagt
     2581 cgcgccgggc gaaagacttc ccacgcctgt ttctctgtcg tggcaccgga atacacttcc
     2641 gcgccaaact cgttatcaca ggcaaaacaa tacagggcaa caccggcaga gattgctgat
     2701 ttgccgttct tacgggggat ttcggtgtac acctcccgga agcggcgcaa ccgggtgcct
     2761 ttattgaccc agccaaacgc acagcagatc acaaatagct gccacggctc cagcgtgatg
     2821 ggcatccgtt tgaatgccca ctcccccttg gtgtgcggca acagctgaat aaatttcgcg
     2881 gcccgttcag ccaggtcctt gtcgaagcgg taacgaaacg acttactttt ttccgccatc
     2941 aggtcatcaa gatggcgctg gcaggcctga atcacaaact ggcaggcaac aatctttccg
     3001 cgcacgacat cccgggcata ctgatttgca gcatttacgt tggggtaaga tttccggctc
     3061 atgattcgat aattttcaga aacgggttag tggctttctt ctgccccgcc aggccaatca
     3121 gacgctggcg gctgctgggg tcgagtccga gcattgcccc cgtgctgctc atctcggact
     3181 cctgttcttt tttggcggtc agctccggat ttttgaccct gccgcccatt gcaccggtga
     3241 tggtgttgcc ctgtatggca atatttttca cggcacgtcg ccagaactca taggccacgc
     3301 accaccgctc aagtaccgcc aggtcagtca cgcacagcag gccctgaccg cagagttctt
     3361 tggttgtcag ttgccacatg atcgtggcga gagggagatc ttcttcagcg aaccactctg
     3421 gtggctcaac acctttgatg ggcgtaaaaa caggttcatc tttgttcagg gctcgcttgc
     3481 cggggtttcc ggccagcgcc ttgcgcgccg ttggcttggg gcgacgcccg gaacgccccg
     3541 ccgttccagc catatgcggc actcctggtt aaatttcatt tttcgcgggt ataaaaaaac
     3601 gatggggcgg gcagtccgga agacgtcagg tcacagggat ttgccccgcc cctccctaca
     3661 agtgagaata attatcaccg gattcgttcg cgcgctgttt tcgctttatg gcagggccag
     3721 cacagactct gcaggttgct gtctgcgtct gttccgccat gcgctttcgg gatgatgtgg
     3781 tcgacggttt tcgcctcgct caccacaccg acacgcagac acaactgaca cagaccttta
     3841 tcgcgcttca gaatacgggc acgaatcacc gtccattttg agccatagcc acgctggtgg
     3901 cggctcagtc cgcgctggtg ctgcacccag ccttcaccgc gatgtttatc gcagtaaccc
     3961 gaactgtctg tggttgtacc tgcacatcca cgcttacggc aggcacgtgg gattagtgat
     4021 ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta cttaatgcac
     4081 tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat cagccgctct
     4141 ctgagggtga aataatcccg ttcagcggtg tctgccagtc gggggctggt tgcattatcc
     4201 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     4261 cgacgaccag cggcaacatc agcgcgcaga gtttcatttt cagctttcgc atcagctaac
     4321 tccttcgtgt atttagcatc gagtgcatta acatcacgct gtctctgctg catgtcagta
     4381 atggttgcgt tcgccagctt cagttctctt gcgtttttgt cgcgctgctc tttgtaggta
     4441 atggcgttat cacggtaatg attaacagcc catgacaggc agacgatgat gcagataacc
     4501 agagcggaga taatcgcggt gactctgctc attgctgccc ccacaaacag acttcacgct
     4561 caatctcacg acgggtcatc agccctttcc attgcttacc gccagcatat gtccagcgac
     4621 gtagctggtc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg
     4681 ttctgaaatt gccagcgccc acgttgtaaa cgaacgagta aagagcgccg cgcgttgttt
     4741 ccggtatatc gactttgatg tacgggttaa tttgtctggc gacagtggca aggtctttat
     4801 tcaggagggc tttgcattct gcttcggtat acgttttacc gagcatgatg tcttttccgg
     4861 tgtgtccgtg acatacagtc catacaccaa tgatatcttt gtatggtatg tagctgacac
     4921 cttccagacc atcgttacca cctgggccag tgattaacac agatgctata gcaacagccc
     4981 caccaccaat agcagctgca acagccttgc gtaatgatgg cgacattatt cacctctcgc
     5041 agccttacgc ttgtcttctc ttattttgaa atacagattt gtcagataag tcagaagccc
     5101 cagaagcaga cttcccagca caccaatcgc agcccactgt gatggactga cctgatccaa
     5161 ccactgcaaa aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcctgttga
     5221 tattttttcc atctgataca tatcccgccc cccgacagac ctgtgctatc ggaaagaaaa
     5281 aaggccatca gcaaaactct gatggcctga atcaccttta ccaatattgt atgaaaaaac
     5341 acgcacgact aattgacaat aattttcatt tccattaaaa aagatcgcgt agcattctta
     5401 attcatgagg aactttaccc gccagcaatc tgagtagcga aagctgttca tccccaatgt
     5461 ttttgctggc gggtcctttt ttctacggtc ctcctccccg gaggggggca tttcattatt
     5521 tttctaatcg ttactggtga gaaggcggaa gaaaaagcca gttcttcgcc cctaaataac
     5581 agcatgattc cagtccatgc aaaatctgat ttccagctca cacataccga gcgctgtaca
     5641 aaaaaccgcc agccaaggca gttaagagtg tgttcccggg gtttgcttag aatattttgt
     5701 aagttgtccg aagagatatt tacaacacca gaatgatgaa tcatcagtcc cctgccagag
     5761 gttcactacg taacctgacc gacaatgcac cattctggtg ctgaaaaaag agcactggaa
     5821 ctgcaacaaa acaacatcac agaacaggag atggtaagga gtcagacatt cccacacaat
     5881 attgtgtcag tgcaaataac aaccttcgtc tcaaatctcg ctggagcggg cagcgggaat
     5941 cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat gcccgcatat
     6001 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctgcctg
     6061 ctgagctaag tcggcgctgg cccaccaccg aggactcgaa cctcgcaccg tcaacttaga
     6121 aggttgatgc tctatccgga tgagctagtg gtggttggtg gcccttgctg gacttgaacc
     6181 agcgacctgg cgattatgag tcgctcgctc tgaccaactg agctaaaggg ccggaggcag
     6241 aataataacc atatgtcatc acatctgcaa actcatctga ccaccagcac gtttaacgtc
     6301 ctgtgccgtt tttcaggtat aaaaaaaccc gctcatcggc gggtttaagc tgtgtggcgt
     6361 agtaaccact cttaacagga tattcaactt tttacgatcg taaagttttc ggaaaaaatt
     6421 tttaaaaccg catcaaccgc tccaccagtt gctctttacg ggcaacgatc cacccgtgtt
     6481 gctccagata aaatttaaac cgttccagag tacataccat cgcatcggcg ggtacctttt
     6541 ccgtgaactc aacctgaccg tgtttatcga agtggctcag taatgcgcat ccatcatttt
     6601 cgatagatgt gttttgtgcc gctggtggct gtttctggtt gaaataacag tcttccagtt
     6661 tttcgaacac ttcccacgcc tgatcggttt cgagcatttt ggcgtgacgg gctgcgccac
     6721 gttctgtcca gaggatgagg gagcgaacgt tacgggcaat tttcacagaa actcttaaag
     6781 agttttcgtt cttcaactct cgtaacgcat caccttctat caggaaaaaa tgtttacctt
     6841 ctataaaccg ttctttgttt cgattgaaat tgtttgtcaa acgctggcgc tctgttccgt
     6901 acaaatcagc cagcagctct gttgtcatga caggaagatg gttatgcgta agtgacggga
     6961 gtttttcaat agacgtgcga ttcatagaca tgtcctttcg aatattttga tttacccctt
     7021 ttgagagggt gaccgggcgc tcaaaaccgt cgaaagtcgg cgggcgtatt tccattgctg
     7081 gtattgtatt agccgcacac ccggtcataa accaagaatt ctggacacaa aaaaaccaca
     7141 tggctatcgg gtgtggatac cgctttcgag gtgttttgag caccgcgggg aaatgtaaac
     7201 tgcaaacctg aaactgtcaa caccgtcagg ttccaatcat cgggtggtga gacgcacagg
     7261 gttggaacta ccgggaaacc gaccggcgag cttttcagct cccccatgcg ccccaccata
     7321 attcagatgt gcgcgtgcat acgacaataa aaaacacgct cgcggcgtgt gtctgtcgcg
     7381 gtctctatcc ggggttccaa tcccgacgcc agattttgct ggcgcgtgag gaatatagcc
     7441 ccggacaatg tgtctggtca agcacctaca taattcgttc tacgtatctg tccatctcca
     7501 gccggatatc aagcatcatc aacatgccat caataacccc ttccgctttc tgcaggcgct
     7561 tgccaataca ggtatccgaa cacccatgtt tccgtgccag actcataaaa gtcattccac
     7621 ctacgtaata atccaccagc aaatcgtgca aatcctgatt tttcttgttc aaccgggcca
     7681 tacagccaca aattatcatt gcatcatcat cagaacactg agggcgtgat ttcactttcg
     7741 acggaattaa ccctttaaaa ccagcagcga tcgacgccca ggatacatct tcatgagtgt
     7801 ttgcagccca agccccccac cgttccataa cctgctgaat atcacgcgcc atcgttatca
     7861 cctgtgattt cataaatctt cacgcccagt cgcccaccag gaacgagctg accgcgcaca
     7921 atattgattt catcaaactg ctcgtcgtct atgagtagtc cggcatgcgt cagcgcatcc
     7981 agtggtgctt tcaggatatt gtccaggtca cgacggcgct tatccggtgg ctctgcaata
     8041 atctttatcg ccagccttcc ggacaggttt aatttcagcc gctgctggcg aacaattagc
     8101 gtcacatcac ggcgataacg ctcaccaact tttgatacaa aatatgtgct gccacgacgt
     8161 cgccagtaag tattcaccgt cggcggataa ggtaaaataa actcatggcg catcagcgca
     8221 gcacctcctg caccattttt tcaaactttc caactttggt ttccagctct gccacacaat
     8281 caacgagctc atccaccgct ttttgtgcgc gatgtttcgc ctgcatcagt tccctgagcg
     8341 ctggcaccat atcccgacga atggcatctt ttgttacacc tgttttttcc agttgttccg
     8401 cctgtcgcaa catttcctgc gcgtgtttac gcaattgttc agaggtaaaa gtcattgtct
     8461 ggttgttcaa aagaaacgct ccatcttact gctgtcggta cgtgtattgc tgtatctgcg
     8521 cggctggggc tgccgcattg gggtggaaag aacctgtgcg ctttcctggt ccacaggcag
     8581 aaaatgtccg ttataaaaac gccggtaaat cgtccccaga gaaccgttac gttgtttcgt
     8641 gatattaatt tctgctatgc ctgtagcctg tgtatccggg ttgtacactt catccctgta
     8701 aagcatcaga atgatgtctg catccgcctc tatttctccg gaatttttca ggtctgagtt
     8761 catgggacgt ttattgggtc tggactccac accgcgggag agctggctca gcgcaatcaa
     8821 cggaaaacca ccggattttg ccaggccttt aagccccttt gagatttcac ccacggcaag
     8881 gtcatgacgc cccgtggttc gggtttttat cagcccgaga taatcaacca ccaccagcgc
     8941 cgtttccgga tgtttaatca gatggtgttt cgttgttgcg catatctcat caatggtcag
     9001 gttcgcctgg tccaccatcc agatattgcg cccggtcatc cgccccaccc cttgtgagaa
     9061 acgcgcccag tcttcatctt caaagtgagc cacagatttc aggcgtgata ctggcatccc
     9121 tccagccgca gacaccatac gttcaccaat ctggatgttc gccatctcca tggtgaacag
     9181 aagcacacca tgcccctgct cagtcacctt gtcgatgatg tccagcgcaa gttcggtttt
     9241 ccccatcgaa ggacgggcgg caatgaatac caggtctccg ggctccatac cgcctgtttt
     9301 tgcgtccagt tcatcaatac cggtcatcaa cgtcctggat ttctccagcc cctgattccg
     9361 gcattcaaca cgctcaacca cttccggaag cacatcatca atatgtaccg gctgaatgac
     9421 gccctttcct gtcgacagtg tgaccatcat gttctgcgca tccttcaggg catcttcagc
     9481 tgcttcacag gtatgcgcat cacgtaattt ctgcagcgcc tcattcagtg ttttttctgc
     9541 atcgcgcagt gcggcattgc gccgcaacgc tgcaacatag tgctccagtg aagacttcac
     9601 ccaggtttta cgcccggtat cagtaatcac cggggcaagt tccggcatct cattacacaa
     9661 cagcacgggg tcaatcactc ctgaaacacg ggcctgtctg cagatgcctg tgtagatatc
     9721 ccgatacgct cgtacagaaa aaatgtccgc cggtagtgtg atcagaatat ccatcacttc
     9781 atgatctgcc ccacgcagaa agaatgcgcc aatgacagcg ccttccaggt catcattacg
     9841 ccagactggt gttgtcatgc agccacacct ctgatacaag aacggtagct gggccagttg
     9901 aacgacaacc agttgcgccc cccgtctgtg atcctgtcgg caatgcgggg gctgatgaac
//
LOCUS       DAJTXQ010000141         9898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000141 DAJTXQ010000000
VERSION     DAJTXQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.141"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            486..1157
                     /locus_tag="OO515_003460"
     CDS             486..1157
                     /locus_tag="OO515_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR6577301.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OO515_003461"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OO515_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OO515_003462"
     CDS             2363..2923
                     /locus_tag="OO515_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6577302.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OO515_003463"
     CDS             2925..3698
                     /locus_tag="OO515_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCR6577303.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OO515_003464"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OO515_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR6577304.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OO515_003465"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OO515_003465"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR6577305.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OO515_003466"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OO515_003466"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6577306.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OO515_003467"
     CDS             complement(6457..6996)
                     /locus_tag="OO515_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCR6577307.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO515_003468"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO515_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR6577308.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO515_003469"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO515_003469"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR6577309.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..>9898)
                     /gene="mhpF"
                     /locus_tag="OO515_003470"
     CDS             complement(9606..>9898)
                     /gene="mhpF"
                     /locus_tag="OO515_003470"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6577310.1"
                     /translation="DIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQ
                     FSGLKTAVWLEVEGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
BASE COUNT         2460 a         2591 c         2403 g         2444 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcat
//
LOCUS       DAJTXQ010000142         9819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000142 DAJTXQ010000000
VERSION     DAJTXQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.142"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO515_003471"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO515_003471"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6577311.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OO515_003472"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OO515_003472"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6577312.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OO515_003473"
     CDS             1829..2101
                     /locus_tag="OO515_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6577313.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO515_003474"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO515_003474"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6577314.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO515_003475"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO515_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6577315.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASARGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO515_003476"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO515_003476"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6577316.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OO515_003477"
     CDS             6114..6665
                     /locus_tag="OO515_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6577317.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OO515_003478"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OO515_003478"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6577318.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OO515_003479"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OO515_003479"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6577319.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2450 c         2618 g         2317 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg
     1561 gctgtggttg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ccgatggcgt
     1921 tgcgttgacc gaggaacaaa aagcgaactg cctgcaactg gtgatgctgt ggcaagctcg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttaggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgcgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacatt taccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagcgaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg ggtgccagtc ctgactggcg
     2761 ataaccgctt caccgcgcga ctggcaccag tcaatcaggc ggttagcgac atccaccgta
     2821 ctgtcacctt ccggcacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgaggggg cattacagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgagcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ccggttggcg
     3241 cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggca gtatacgcca tcgtgtcggt
     3661 gccgtgcagg atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcttctggcg tcatatcaga agaatccatc agcggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgacg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgccagc tctgctgctt tggcgaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatctg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtta gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc tgccggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatgtca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaaa tccaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacacaac cggcacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccgtactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgacggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ttttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgcgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attatcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgctcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgctgc agccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 cagccatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gtggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagt
//
LOCUS       DAJTXQ010000143         9565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000143 DAJTXQ010000000
VERSION     DAJTXQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.143"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            88..1344
                     /locus_tag="OO515_003480"
                     /pseudo
     CDS             88..1344
                     /locus_tag="OO515_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1347..2675
                     /gene="ssnA"
                     /locus_tag="OO515_003481"
     CDS             1347..2675
                     /gene="ssnA"
                     /locus_tag="OO515_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR6577320.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2726..3505
                     /locus_tag="OO515_003482"
     CDS             2726..3505
                     /locus_tag="OO515_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR6577321.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3502..6441
                     /locus_tag="OO515_003483"
     CDS             3502..6441
                     /locus_tag="OO515_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR6577322.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6374..6688
                     /locus_tag="OO515_003484"
     CDS             6374..6688
                     /locus_tag="OO515_003484"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6577323.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6724..7074
                     /gene="ghxQ"
                     /locus_tag="OO515_003485"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6724..7074
                     /gene="ghxQ"
                     /locus_tag="OO515_003485"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7143..7631)
                     /locus_tag="OO515_003486"
     CDS             complement(7143..7631)
                     /locus_tag="OO515_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6577324.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OO515_003487"
     CDS             complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OO515_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCR6577325.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2308 a         2564 c         2491 g         2202 t
ORIGIN      
        1 gatccttaac tgatcggcat tattcctcag ttccgtattc ctgcagagtt aattcagcac
       61 gatatcgatt ttgttgccgc tcacggcgtg aaatttgagt atggctgctc acccgattta
      121 accgttgaac agttaaaaaa tcagggcttc cactatgttc tgattgccac cggcactgat
      181 aaaaatagcg gtgtgaaact ggcgggcgac aaccaaaatg tctggaaatc actccccttc
      241 ctgcgtgaat acaacaaggg tacagcgctc aagctgggca aacatgtggt cgttgtcggg
      301 gcgggtaaca ccgcaatgga ctgcgctcgt gcggcgttac gcgttccagg cgtagaaaaa
      361 gcaacgatcg tttaccgtcg ttcactacaa gagatgcccg catggcgcga agagtatgaa
      421 gaagcgttgc acgacggcgt agagttccgt ttcctgaata atccggaacg tttcgatgct
      481 gatggcacct taaccttgcg cgttatgtcg cttggcgaac cggatgagaa aggtcgtcgc
      541 cgtccggttg aaaccaacga aacagtaaca ctgcatgtag acagcctgat caccgccatt
      601 ggtgaacagc aggatactga agccctgaat gcgatgggcg tgccgctgga caaaaacggc
      661 tggccagacg tcgaccataa tggcgaaact cgtctgactg acgtctttat gatcggcgac
      721 gtacagcgcg gaccatcctc cattgtcgct gctgtcggaa ccgcgcgtcg ggcgaccgat
      781 gccatcctta gtcgggaaaa tatccgttcc caccagaacg ataaatactg gaacaacgtc
      841 aatccagcgg aaatctatca acgtaaaggc gatatctcta tcacgctggt gaacagtgac
      901 gatcgtgacg cgtttgtcgc ccaggaagcc gctcgctgcc tcgaatgtaa ctacgtttgc
      961 aacaagtgtg tggatgtctg cccgaaccgc gccaacgtct ccattgcagt ccctggcttc
     1021 cagaatagat tccagacgct gcacctcgac gcttactgta acgaatgcgg caactgcgct
     1081 cagttctgtc cgtggaacgg taaaccatac gaagacaaaa tcaccgtctt cagcctggcg
     1141 caagactttg ataacagcag caccccaggc ttccttgtgg aagattgccg ggtacgagta
     1201 cgtctgaata accaaagctg agtgttaaac atcgacagcg aaggtcagtt taacaacgta
     1261 ccaccggagc tgaacgatat gtgccgcatc atcagccatg tccaccagca tcatcattat
     1321 ctgctgggcc gcgtggaggt gtaatcatgt tgattctgaa gaatgtcacc gcagtgcagt
     1381 tacacccggc gaaagtgcag gaaggcgttg atatcgctat cgaaaatgac gtgattgtcg
     1441 ctatcggcga tgccctgacg caacgctacc ccgatgccag ctacaaagag atgcatggtc
     1501 ggattgtgat gccgggaatt gtctgctcgc acaaccattt ttactcggag ctttcccgcg
     1561 gaattatggc aaacatcgcc ccttgcccgg atttcatctc aacgctgaaa aatctctggt
     1621 ggcggctcga tcgcgccctt gatgaagagt cgctctatta cagcggactg atttgttccc
     1681 tggaagcgat taagagcgga tgtacatcgg ttatcgatca ccatgcctcg ccggcgtata
     1741 tcggcgggtc gctctccaca ttgcgcgacg catttttaaa agttggcctg cgcgcgatga
     1801 cctgttttga aactactgac cgtaacaacg gcatcaaaga gttgcaggaa ggtgtagaag
     1861 aaaacatccg tttcgcccgt ttgattgatg aggcgaagaa agcgacaagc gagccgtatc
     1921 tggtggaagc acatatcggt gctcacgcgc cgtttaccgt gccggatgcc ggtctggaga
     1981 tgctgcgtga agccgtgaaa gccacaggcc gtggtttgca tattcacgct gcggaagacc
     2041 tttacgacgt ttcctacagt caccactggt acggcaaaga cctgctggca cgactggcgc
     2101 aattcgacct catcgacagc aaaacgctgg tcgctcatgg gctgtacttg tcgaaagatg
     2161 acatcgccct actcaatcag cgcgatgcgt tcctggtgca taacgcccgt tcaaacatga
     2221 acaaccatgt cggctacaac catcacctta gcgacatccg caacctggca ttgggaacgg
     2281 acggcattgg ttcggacatg tttgaagaga tgaaatttgc cttctttaaa catcgcgatg
     2341 cgggtggtcc gctgtggcct gacagttttg ccaaagccct gactaacggt aacgaactga
     2401 tgagccgcaa ctttggcgcg aaatttgggc ttctggaagc cggttacaaa gctgatttga
     2461 ccatttgcga ttacaactcg ccgacgccgc tgctggcaga caatatcgcc gggcatatcg
     2521 ctttcggtat gggctcaggc agcgttcaca gcgtgatggt caatggtgtg atggtctatg
     2581 aagaccgtca gtttaacttc gattgcgatt ccatttatgc acaagccaga aaagccgctg
     2641 ccagtatgtg gcgtcggatg gatgcgctgg cataaatgac agatgccctc ttcccgcagg
     2701 gaagagggtt aaataaggaa agaagatgat tgaacaattt ttcaggcccg actctgtcga
     2761 acaggcgctg gaactgaagc gccgctacca ggatgaagcc gtctggttcg ccgggggcag
     2821 caaactcaat gctacaccaa cccgtaccga taaaaagatt gccatttcct tacaggatct
     2881 ggaactggac tgggttgact gggataacgg tgcactgcgg attggtgcaa tgtctcgctt
     2941 gcagccactg cgtgatgcgc gctttattcc tgcagcactg cgtgaagccc tcggttttgt
     3001 ttactcacgc catgttcgta atcagtcgac cattggtggt gaaatcgccg cccggcagga
     3061 agagtcagtg ctgcttcccg tcctgctagc actggatgct gaactggttt ttggcaacgg
     3121 cgaaacgctg tcaatcgagg actacctggc ctgcccatgc gatcgcctgc taaccgaaat
     3181 tatcattaaa gatccgtatc gcacctgtgc gacccgcaaa attagccgtt ctcaggcagg
     3241 tttaaccgtc gtgacggcag ccgttgcaat gacagaccac gacggtatgc gaattgcgct
     3301 ggatggcgtc gccagtaaag cactgcgtct gcacgatgtc gaaaaacaaa atctggaagg
     3361 caatgcactt gaacaggctg tcgccaacgc cattttcccg caggaagatt tgcggggcag
     3421 cgtggcctat aaacgctata tcacgggagt tctggtagcc gacctgtatg ccgactgcca
     3481 acaggctggg gaggaagccg tatgatcatc cactttactt taaatggcgc gcctcaggag
     3541 ctaaccgtta atccaggcga aaacgtgcaa aagctgttgt ttaacatggg aatgcactct
     3601 gtacgcaaca gtgatgatgg cttcgggttt gccggttctg acgcaataat ctttaacggt
     3661 aatatcgtta acgcgtcctt gcttattgcc gcacagttag agaaggcaga tattcgtacc
     3721 gcagaatctc tgggtaaatg gaacgagtta agtctggttc aacaggcaat ggttgatgtt
     3781 ggcgtggtgc agtctggtta taacgatcca gctgcagctc tgattatcac cgatcttctc
     3841 gatcgcatcg ccgcacctac ccgcgaagag atcgacgacg cgctttctgg tttgttcagc
     3901 cgcgatgcag gctggcaaca atactatcag gtcattgaac tggcggttgc acgtaaaaat
     3961 aatccgcagg ccaccattga tatcgctccg actttccgtg acgaccttga cgtcattggc
     4021 aagcattatc ctaaaactga tgccgcgaaa atggtgcagg cgaaaccctg ctatgttgaa
     4081 gactgcgtaa cggctgacgc ctgcgtcatt aaaatgttac gtagcccaca cgctcacgca
     4141 ctgattactc atctggatgt cagcaaagct gaagccttac cgggcgtcgt tcacgttatt
     4201 actcacctga attgccctga tatctactat accccggggg gtcagagcgc accggaaccg
     4261 tcaccgcttg accgccgtat gttcggcaag aaaatgcgtc acgtcggcga tcgtgttgct
     4321 gcggtcgtcg cagaaagtga agaaattgcg ctcgaagcac tgaagctcat cgacgttgaa
     4381 tatgaagtgc ttaagccggt aatgtcgatc gacgaagcaa tggcggaaga tgcgcctgtc
     4441 gtgcacgatg aaccggtggt gtatgttgct ggtgcgccag atactctgga agacgataac
     4501 agccatgcag cccagcgcgg cgagcatatg atcatcaact tcccgatcgg ttctcgccct
     4561 cgcaaaaata tcgccgccag tattcatggt catattggcg atatggacaa aggctttgcc
     4621 gatgccgatg tgatcattga gcgaacctat aactcaacgc aggcacagca gtgcccgact
     4681 gaaacacata tctgctttac ccggatggac ggcgatcgtc tggttatcca cgcctccacc
     4741 caggtaccat ggcacttacg ccgccaggtc gcgcgcctcg tgggcatgaa acagcataaa
     4801 gttcatgtca ttaaagagcg agttggcggc ggttttggtt ccaaacagga catcctgctg
     4861 gaagaagtgt gcgcctgggc aacctgcgtg accgggcgtc cggtactatt ccgctacacc
     4921 cgtgaagaag agtttattgc taacacctct cgtcacgtcg cgaaagtcac cgtcaaactg
     4981 ggagcgaaaa aagatggtcg cctgacggca gtgaagatgg atttccgcgc caacactggc
     5041 ccttacggca accactcact caccgtaccg tgtaacggac cggcgctgtc gctgccgtta
     5101 tatccgtgcg ataacgtcga tttccaggtc accacctact acagcaacat ttgcccaaat
     5161 ggtgcttatc agggttatgg cgcaccgaaa ggtaacttcg ctatcaccat ggcattagcg
     5221 gaactggctg aacagttaca gatcgaccaa ctggaaatta tcgaacgtaa ccgggtacac
     5281 gaagggcaag agctgaaaat tctcggtgca atcggtgaag gtaaagcgcc gacctccgtt
     5341 ccttccgccg ccagctgtgc actggaagag atcctgcgtc agggtcgcga gatgatccaa
     5401 tggtcgtcac cgaaaccgca aaatggtgac tggcacatcg gtcgcggcgt cgccattatc
     5461 atgcagaaat cgggtatccc ggatatcgat caggctaact gcatgatcaa actggaatcg
     5521 gacggcacct ttatcgttca ttctggcggt gcggatattg gtactggtct ggatactgta
     5581 gtgacgaaac tggcagcaga agtgctgcac tgcccaccgc aggacgtgca tgttatctcc
     5641 ggtgataccg atcatgcgtt gtttgataaa ggcgcatatg cctcgtccgg tacttgcttc
     5701 tcgggtaacg cggcgcgttt ggcagcggaa aatctacggg agaaaattct gttccacggc
     5761 gcgcaaatgt tgggtgaggc agtggcagat gttcaactgg caacgccggg cgtcgtgcgc
     5821 ggcaagaaag gcgaagttag tttcggggat attgcccata aaggcgaaac cggcatcggc
     5881 tttggttcac tggtgggaac tggcagttat atcacgcctg atttcgcctt cccgtatggc
     5941 gcaaacttcg ctgaagttgc cgtcaacacg cgtacgggtg aaatccgcct ggataaattc
     6001 tacgccttgc tggactgcgg tacaccggtc aacccggaat tagcattggg acaaatctac
     6061 ggtgctaccc tgcgagctat cggccacagt atgagcgaag agatcattta tgacgccgaa
     6121 ggtcacccgt taacgcgtga tttacgcagt tacggcgcac cgaaaattgg tgacattccg
     6181 cgtgatttcc gcgctgtgct ggtgccgagc gacgataaag tcggcccgtt cggggcgaaa
     6241 tcgatctcgg aaatcggtgt aaatggcgca gctccggcga ttgctaccgc aattcacgat
     6301 acatgcggca tctgggcaga agaaagtcaa agtgggcatg ggaacggata tcggtgccgg
     6361 aacaactttc aacatgctgc aaacgctgaa cgaagcctac aaagtgttgc aattacaagg
     6421 ctatcgcctc tcggcatatg aagcgtttta cctggccacg ctcggcggag cgaaatctct
     6481 gggccttgac gatttgattg gcaacttttt acctggcaaa gaggctgatt tcgtggtgat
     6541 ggaacccacc gccactccgc tacagcagct gcgctatgac aactctgttt ctttagtcga
     6601 caaattgttc gtgatgatga cgttgggcga tgaccgttcg atctaccgca cctacgttga
     6661 tggtcgtctg gtgtacgaac gcaactaata ataaaacttt aacatcctcg tgaggacatc
     6721 attatgtctg gagacatcct acaaacaccg gacgcaccaa agccacaggg cgcgctggat
     6781 aattatttta aaattaccgc tcgtggcagt accgttcgtc aggaagtact ggctggctta
     6841 acgacctttc tggccatggt ttattccgtt atcgtcgttc cgggaatgct gggcaaagca
     6901 gtttttcctc ccgcagctgt gtttgttgcc acctgtctgg tcgcgggctt cggctcgttg
     6961 ctgatggggt tgtaggccaa tttgccaatg gcgattggtt gtgcgatttc cttgacggca
     7021 tttaccgcat tcagtctggt actcgggcaa caaattagcg ttcctgtcgc actggtcgca
     7081 ttttacgcgg gtggttgggc aatctaatag attctccgcg cctttcagcg cggagatttc
     7141 tttcacagag gattctcacc gctggcaaca atacgctgct ggcggactcg cctgagttct
     7201 ctttcagtaa gcaactgcag cgcctgtgtc gggcaagatt caacacaggc tggcccctct
     7261 tcccgctgtt cacagagatc gcatttcaca atttgttgtc gggtatcccc cggcaacgac
     7321 tgaatggtga tcattccaaa cgggcacgca ctgacgcaac tctgacagcc aatacaacgg
     7381 gcagaattgg tctgcaccac ctgctcgccc atcgtcagcg cccccacggg gcaagcgcca
     7441 acacaagggg cgttttcaca ctgatggcac atcaccggcg cactgatgct gtccagtcgc
     7501 tgtaccttca gccggggtaa aaagacatcg gcattcaact cctgttctga agggtgagcg
     7561 accacacagg ctacttcaca agtacggcag ccaatacagt cagccggatt aacgataatt
     7621 aacgatttca tcacgatgcc ttcgtatcaa acagagttaa catatcgcgc gccgcctgcc
     7681 ttcctgcggc cattgcagtg acaaccagat ccgcgccatg aactgcatca ccaccagcaa
     7741 agactttttt cagatgcgtc tgggtaggta aatacccgac gtcaccggtt tgaatcaggc
     7801 cccatttatc gagtttaatt ccgctgccct gcaaccacgg catggcatgc gcctggaaac
     7861 caaaggccat aatcagaaca tcggcgggca attcaaactc tgaacccgct accggacgag
     7921 gacgacggcg accatccggc cccggctcac ccatggcggt acgaatcagg cccaccgcag
     7981 ttaaacgccc atcttcgtca caagcgatat attgcggttg aacattgaac tgaaactcga
     8041 caccttcctc gcgcgcatta accacctctt tgcgcgagcc cggcatactg acttcatcac
     8101 gacgatacgc gcaggtcacg ctggcagcat tgaggcgaat ggaagtccgc aaacaatcca
     8161 ttgtcgtatc gccaccgccc aataccacga ctcgcttacc ttccacgtcc gtcagcggat
     8221 actcttcaga ctccggcaat cccatgagct ggcgggtatg ggcagtcagg aacggtagag
     8281 cctgaatgac accgggcgca tcttcatgcg gcagatctgc tcgcatcatc ccgtaagtcc
     8341 ccacgccgat gaaaactgca tcatattcag aagttaaatc gctaaaggta atatcgcggc
     8401 caatttcaca gttaagatga aagtcgattc ccattgcggt gaatatctct cgccgctggc
     8461 ttaataccgt tttatcgagt ttgaaaggag gaatgccaaa agtcagcata ccgccaattt
     8521 ctggatggcg atcaaagaca tcaacctgaa ctcccgcgcg cgccagaata tcagcacacc
     8581 ctaaccctgc tggcccagcg ccaatcaccg ccactttttc gatacgggga acaactttgc
     8641 tgacatcggg acgccagccc atcgccagcg cggtatcggt gatgtagcgt tccagattac
     8701 cgatagagac tgcgccagag tgatctttca aagtacatgc gccttcacaa agacggtcct
     8761 gtgggcatac cctgccgcag atttctggta aggaactggt ctggtggcaa agttctgccg
     8821 cttcaataat ctttccttcc tgtaccagac ggatgtaatc cggaatagcg ttatgcagcg
     8881 ggcaatgcca gttgcagtta gctttttcgg cacaataaac acagcggtca ctctcataag
     8941 tcgcttgcag tggatccagc ccgcaataga tttcgccaaa gtgggttttc cgttcactcg
     9001 ctgaaatttt atctgcgcct ttacgcgagt taacggggag caacgctgca ctgcgagatg
     9061 gctgagcgtc tgatgacgct tttcctgctg ccgttttgcg ctggcgagcc acctttatcc
     9121 actgtaaccc tttatcgtcc atcagtcgta acgcctgtgt tgggcagact tcaatacagg
     9181 cttgcgtgcc ggaactgcgc tggttacaaa ggtcgcattt ctgcgcaatc gtatcgacca
     9241 tctcaacgac gccaaagggg caagcgattg cgcatctttt acagccaata catttttgct
     9301 cgtccagttg tacgctatcg gactggaaag tcagagcatt aaccggacaa gccgtaacac
     9361 aaggggcatt gttgcagtga tggcaggcca ccggattcgc agcctggcct ttccctacaa
     9421 cgtggatacg cggtcgaaag tcactgtgac tcagcggcca gttttcttga ttgtgtgcca
     9481 ccgcacaggc aatttcacaa gcatgacagc ctatacattc cgcagcttca gcagcgataa
     9541 acttattcat ttgcatccct ttcat
//
LOCUS       DAJTXQ010000144         9503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000144 DAJTXQ010000000
VERSION     DAJTXQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.144"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..2199
                     /locus_tag="OO515_003488"
                     /pseudo
     CDS             223..2199
                     /locus_tag="OO515_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="OO515_003489"
     CDS             2192..3328
                     /locus_tag="OO515_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6577326.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="OO515_003490"
     CDS             3325..5325
                     /locus_tag="OO515_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6577327.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="OO515_003491"
     CDS             5447..5908
                     /locus_tag="OO515_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6577328.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYT
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="OO515_003492"
     CDS             complement(5949..6419)
                     /locus_tag="OO515_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6577329.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO515_003493"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO515_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6577330.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO515_003494"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO515_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6577331.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="OO515_003495"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="OO515_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9382..>9503
                     /locus_tag="OO515_003496"
     CDS             9382..>9503
                     /locus_tag="OO515_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6577332.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEV"
BASE COUNT         2321 a         2504 c         2438 g         2240 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctaca cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
     9481 cgttatgacg atgtagaggt gat
//
LOCUS       DAJTXQ010000145         9439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000145 DAJTXQ010000000
VERSION     DAJTXQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.145"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..717)
                     /locus_tag="OO515_003497"
     CDS             complement(<1..717)
                     /locus_tag="OO515_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HCR6577333.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLP"
     gene            complement(851..1687)
                     /gene="bglG"
                     /locus_tag="OO515_003498"
     CDS             complement(851..1687)
                     /gene="bglG"
                     /locus_tag="OO515_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6577334.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(1973..2698)
                     /gene="phoU"
                     /locus_tag="OO515_003499"
     CDS             complement(1973..2698)
                     /gene="phoU"
                     /locus_tag="OO515_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6577335.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(2713..3486)
                     /gene="pstB"
                     /locus_tag="OO515_003500"
     CDS             complement(2713..3486)
                     /gene="pstB"
                     /locus_tag="OO515_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6577336.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(3577..4467)
                     /gene="pstA"
                     /locus_tag="OO515_003501"
     CDS             complement(3577..4467)
                     /gene="pstA"
                     /locus_tag="OO515_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6577337.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(4467..5426)
                     /gene="pstC"
                     /locus_tag="OO515_003502"
     CDS             complement(4467..5426)
                     /gene="pstC"
                     /locus_tag="OO515_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6577338.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(5513..6553)
                     /gene="pstS"
                     /locus_tag="OO515_003503"
     CDS             complement(5513..6553)
                     /gene="pstS"
                     /locus_tag="OO515_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6577339.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(6801..7874)
                     /locus_tag="OO515_003504"
     CDS             complement(6801..7874)
                     /locus_tag="OO515_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6577340.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(7885..>9439)
                     /locus_tag="OO515_003505"
     CDS             complement(7885..>9439)
                     /locus_tag="OO515_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6577341.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2467 a         2418 c         2107 g         2447 t
ORIGIN      
        1 cggtaaccac gcattaagtc ggcgttccag aatgctgcac aaccaggcag aaaaaataat
       61 gggaataacc gatgacgagt aattcaacaa tgtgaccgga atacccagga aatccagccc
      121 cagcgcatcc gctttttgcc cgttctcgaa agcagtcaga attaatggat gcactaacgc
      181 tccaccaatc accatggcag taaatggatt accgccaaag cgtttccccg cggtgtatcc
      241 caggattatc gggaagaacc aaaacaaggc atcactggcg ctgaataaaa ttaaataagt
      301 accactttgt tcggtcgtcc actgaaaagt gagcgccaga gccagcatac ctttcaggat
      361 cccggttgcc gccatcaaac cgatcagagg cgtaaaaata cctgaaataa cataaacaaa
      421 gcggtttagc agattacctt tctcatcatt ttccggtgcc tgttgcgctt tttcgccaag
      481 gcctgccacg ctgttgaccg ccaggaagac atcggccaca tggttaccta tgaccacctg
      541 aaactggcca ccgctttcca ccaccataat aataccgggg gtgtttttca gtacctcagc
      601 ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat gcatcagact
      661 aacaatgtta tctgcgcccc cgactcctgc gactattttt ctggctaact ccgtcataac
      721 ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaaa ctgaaaaaaa cggcctgacg
      781 tgaatcaagc aatttttttc aggttttgcc cgcttagtgc ggtaacaatc ctttactcag
      841 taataatatt tcagtgttct ttgcgcacgc gctctatatt tatggctaaa aacataatct
      901 ctgcgggtga aattttacgt tgatactgca aaccaataaa aatggcgatc cgttccgcac
      961 attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg taatgattca tcactatcgt
     1021 taattgaagc atgttcaaga atacgccagg ataaaaactt cagatgtgta accagtcgct
     1081 gataactcaa gctttcttcc tggtaattaa ggctgaactg aaattttatt aattgcagca
     1141 tttcgcgcat taactgcgtg acacctgcaa catcctccat atttccgctc atttgggcac
     1201 tgaccagatg catggcaata aagcccactt catctttcgg taactgcacg cccaaccgtt
     1261 tatcaataat ggttaacgct tcttccccta gctggaactc tttcgggtaa agccgctgga
     1321 tatcccacag caacgggttg ggcagcaaca cgttttgctg aaagcgttta atcgcaaact
     1381 ggcaatggtc agttagcgag atataaatac tgtcctgtaa ttttcccaag cgctcctgcg
     1441 ctaaagagat aatacgatca caggttgcca tcacctcaag aggaatatga cttaagagtt
     1501 cgcttaatcg cccgttcagt tcatgactgc tcaaggcata ctctttttct attccacttg
     1561 agttaattct ttcgccagcg catttttgaa agccaattcc tcgccccatg acgacttttt
     1621 cccgctgttg atcatcaata accaccacaa cattattgtt gagaattttg gtgatttgca
     1681 tgttcatagc aaggaccttt tttataaaca aaaaaaaccg acttcaccag tattctctgg
     1741 ttatgtcagg ttttgcctgc gaatgcagta acaatccagt catttattaa tgttttttat
     1801 aacgaacatc caggttcgga aattaattta attgcgtgca gagaaaatag caatgcgcta
     1861 ttgataaaaa tatgaccatg ctcgcagtta ttaactttgt gtaattttag gaatttataa
     1921 agttatatat aacaaatccc aataattaag ttattgggat ttgtctggtg aattatttgt
     1981 cgctatcttt tcccgccagc agtttatcca gctcatcacc gcctacatga cggaaatcct
     2041 gccccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg ccaatacgtt
     2101 cgatagaacg cgcgcagaac agcgcagtaa gtacgctcgg aatggtacgc gaatcttcca
     2161 tcatgtaggt catcagttga cgaacaatac cttcgtattc ctgatcgact tttttatctt
     2221 cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc agcacgtcgt
     2281 gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac ggctgatgct
     2341 gctgggagaa tttctccagc gcagtacggc agattttgtc cgccacgtcg ccaatacgct
     2401 ccagctcggc aatggtttta ctgatcacca taaccagtcg caggtcgctc gccgtcggct
     2461 gacgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc atgttgacgt
     2521 tcttgtcgcc ttcgatcacg cgcttcgcca gatcgctgtc ctggttatgc atcgcggtga
     2581 ttgcatcaga aagctgctgc tccaccatgc cacccatggt catcacctgc gtgcggatac
     2641 tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga ctgtccataa
     2701 cgcactcctg aattaaccgt aacgaccggt gatgtagtct tctgtttgtt tcttcgctgg
     2761 cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt acataaacgc
     2821 cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtaacgatca ccacggtgta
     2881 atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatgg ggtcgagcgc
     2941 cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac gcgcaataca
     3001 cagacgctgt tgctgaccac cagagagaga gtaaccgctc tggtgcaatt tatctttggt
     3061 ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca tgtcggcacg
     3121 ggagagcttc tcaaacagac gaacaccaaa agcgatgttg tcgtagatgg acatcgggaa
     3181 cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagtgcga tatcctgaga
     3241 gttggtcagg atgttgtcgc catcaagcag aatttcacct tctgcgcgct gctccggata
     3301 cagttcaaac attttgttga aggtacgcag cagcgtcgat ttaccgcagc cggacggccc
     3361 gataaacgcc gttacctggt ttttagcgat atccaggttg atgtttttca gggcatggaa
     3421 tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag tttcaaccat
     3481 actcattgca atcttttcct catcgtacct gatgcttttc gctaatcagg cttaccgtag
     3541 gccgggtaag gcgttcacgc cgcatccggc aaagtgttaa ccgtgtttat tcttcgcaaa
     3601 aacaacgcgc gccagaatgt tcagcagcag gacgcacagg gtaatgatca ataccccggc
     3661 ccaggccagt tgttgccatt ccgcaaacgg gctcatcgca aacttaaaga tcgtcaccgg
     3721 caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga gcgcggtaaa
     3781 cagcagcggc gcggtttcac cggcaatacg ggcaatcgcc agcaggatac cggtcataat
     3841 cccggacacc gacgctttca gcgtaatcgc ggagatcatc ttccacttcg gtgtacccag
     3901 cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct cggtggtgcg
     3961 gataacgatc ggcacctgca acagcgccag cgcaattacg cccgcccagc cggagaagtg
     4021 ctccatctgt gccaccacga tggtgtagac aaacagacca accacaatcg acggcacgga
     4081 gagcagaatg tcgttaatga agcgaatcac ttctgccagc caggatttac gaccatattc
     4141 cgccagataa atccccgcca taatgcccag cggcgtaccg aataccgtgg cccacaaaat
     4201 taacagcccg ctacccgcca gggcgttcgc cagaccacca ccttccgtat tgggcggcgg
     4261 cgtcatttca gtgaacagcg ccagcgacat accgtcgata ccgcgagtga tggtggacat
     4321 taaaatccag atcagccaga acaggccgaa ggccatcgtc gccatcgaga gcgtcagcgc
     4381 aatacggttt ttgaggcggc gacgcgcctg cattttgcgg cgagattcag ccagcgccgc
     4441 agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc caggcgcata
     4501 atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat caggcccagt
     4561 tccatcagtg cggcaacgtg cagaccggat tccgcttccg caaattcgtt cgccaacgca
     4621 gaggtgatac tattgcccgg catgtacagc gaggcgctgt cgagctggta ggtgttaccg
     4681 atgataaagg tcaccgccat ggtttcaccg agcgcgcggc ccagccccag catgatgccg
     4741 ccgataacac catttttggt gaacggaaga acgatacgcc agataacttc ccaggtggtg
     4801 cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa cacatcacgc
     4861 attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc cgcgaggata
     4921 ccgataccaa atgcggggcc agagaacagc gcgccaacaa tcgggatatt cgacatgata
     4981 ttgccgaccg gctcctgaaa gtaaacggcg aacagcggcg caaagataaa caggccccac
     5041 atgccgtaaa cgatacttgg aatggctgcc agcagctcaa tggcgatacc cagcgggcgt
     5101 ttcagccagc caggcgcaag ctcagtcagg aacagggcga taccgaaact caccgggacg
     5161 gcgatcagca gcgcgataaa cgaagtcacc aacgtaccgt agatcggcac cagcgccccg
     5221 tagatatcgt tcggtgcatc ccactctttg gtccatagga aagccagacc aaatttctga
     5281 atgctcggcc aggaggagat gatcagagag acaataatgc cacccaacat caatagcaca
     5341 atcagcgccg ccagttttac cagcacgctg aaaattatgt cgcccttttt acccggtggg
     5401 ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt ttacaaagtc
     5461 attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg cctggagttt tattagtaca
     5521 gcggcttacc gctactgtct ttaatattgg tcttccacgc agcgcgaacc tgttcaacta
     5581 cactatccgg caggctggcg taatccaggt cgttcgcctg tttagccccg gttttgtacg
     5641 cccagtcgaa gaatttcagc acttctgtgc cttgttctgg tttcttctga tctttgtgga
     5701 tcagaatgaa cgtggtagag gtaataggcc atgcatcttc gcctttctgg ttggtcagat
     5761 cctgagcgaa ggttttgctc cagtctgcac cttttgctgc attagcgaag ttttcttcgg
     5821 tcggactaac cggtttacca tcagcggaga tcagtttggt gtacgccagg ttgttctgct
     5881 tcgcgtaagc atattcaaca taaccaattg cacccggcag acgctgaacg aacgcggcga
     5941 taccgtcgtt acctttaccg cccagaccga tcggccattt tacggtagag ccagtaccaa
     6001 cgttgttttt ccactcttcg ttcactttcg ccaggtagct ggtgaagacg aaggtagtcc
     6061 cggagccatc tgcgcggcgt actacagcaa tgttttgtga aggcagtttc agacccggat
     6121 tcagtttggc gatggtttca tcatcccact tcttgatttt gcccaggtag atgtcgccga
     6181 gggttttacc atccagcacc agttcgccag acttcagccc tggaatgtta accgccagca
     6241 ccacgccgcc aatcacggtc gggaactgga acagaccttc ctgagccagt ttttcgtcag
     6301 acagcggcga gtcagaggca ccaaaatcaa cggtattagc gataatctgt tttacgccac
     6361 cggaagaacc gataccctgg tagttaactt tattaccgat ttctttctgg taagtgtcag
     6421 cccatttggc atacaccggc gcagggaagg ttgcacctgc acctgtcagg cttgcttctg
     6481 caaacacaga gaaagcgctc atcgataagg tcgcggcgac aacagttgcg acggtggtac
     6541 gcataacttt cataatgtct cctgggagga ttcataaagc attgtttgtt ggctacgaga
     6601 agcaaaatag gacaaacagg tgacagttat atgtaaggaa tatgacattt ttatgacaga
     6661 aaggaattaa caaataactt actgatttta aatgtgaaga tgaaaatccc acatgtcatc
     6721 tttttgtcat gatgcattta aaagtgataa agaaatgggt gagatgacaa cgatgtgatg
     6781 aacaatcgtt gtcagaccta ttagcgcaca atctcaacag ttatcgtcgc tgttgcagta
     6841 aattgccccg gggctgggcg agctccagta aggctcgcag gagcagctga aaaagttaca
     6901 ctacctgatt tttcaccata cactgttgat ttacccatgt ctgttggcaa ctcaccaccg
     6961 ttcactttga tttccttatt atttttgtcg taaattttta tccccacgtt agagtttgaa
     7021 gttacgacca ttgaattgtc cgtattgggt tcggtagcat aaaaagacgt actgatatca
     7081 tcggtattca gtatccccag gttttcacac tcaatagtga ctgttttagt aattttatgt
     7141 gttgttaccg cacttccaac aacagatgaa aaatctgcag ctggtatttc tttaaaatca
     7201 aaataaactg gctgtaaatt atttatttcg caactttgcg gagctgtaat atcgccccga
     7261 atataaacat cagccatcgg ctgtgagtaa tccaccgggg tgtcacggct aattgttcca
     7321 tacaatgtca ctatctttgt ttcaggaata atcaattttc ctgcaaatgt cttcttgata
     7381 taaaaatata ctatcgcttt acccccactg gatatactta aaggttcaat gcggttgcaa
     7441 ttgtactttg tattaccaga cgggttgtta gcatgatatt caaaaggtgc attcacatac
     7501 ccaaccccaa gaatatctat agcaatccca acatcaaggt tcggattaag atcataataa
     7561 ttaatattag agtgaacccc agcataagtt aatgcctggt ttatttttga cgtataatag
     7621 atatagggga attctcctac attaccagta tcacaatgac agcgtccagg atatgaggat
     7681 ccattagtta cagtggtatt aaatgtcgtt ccaaccttat tttcgttcgc ggtaataaga
     7741 tatcccgaga aatcaagcgt acggtgaaat acgccattat caggggtaca gataccttta
     7801 ggagctgcaa gtgctacact acttaacgag agcataaaca cagcaaagca ccactgtttg
     7861 atatacttgt tcataaaaat ccttttaatg acatgtcaca ccctgttcaa ttaatccgct
     7921 ctcactttct ggtgtcgaaa gtgaataatt gaagatgcat tttgttgagg cgttttctcc
     7981 ccaggataac gtaacctgtc ctttttctgg tagaccagaa agataaagtt cgccacggtc
     8041 accgacaatt cctgaatctg ccaaacttgc atcttgtatc gcagcaatgg ctccaaaagg
     8101 taccggttta ccatttgcat ttaacactct gaagagaacg cgataaccca catgagtcac
     8161 aaactccgct ttaaccactg caccacgcgt gggaaccatc gtaacgacgg tattatcaag
     8221 ttctatattc gacttcgaga aatagttatc gctgattgct acccgatttt catcataagg
     8281 gtttagctgt gtcttgaccg tatagccgcg ccagtccgtc gcagcaccgc gcatattatc
     8341 tatttctaac ccggcggccc caggtgcttt aacaagagca atagtttcac ctaactcctg
     8401 acttaacgtt accccttcgc tgtgtgccag aattccccct ctgataccat aattcagcct
     8461 ctgggaattt tggctgtagt tatagctgcc gttcaccgtg ccataagagc catcataggt
     8521 cagggacata ttaccgccaa cctcatcatc atgactgtta taggactgca ttaaattcca
     8581 tgaaaggtta tttctttcca gcgccgtacc gctgagtccg agttgatgtg agctaccgga
     8641 attatcgttt tgcgaaacag aataggtggc gtaactatta ccccaccagc ccaataaagg
     8701 aacagataaa gagagcatgt agatctgctc actggtgtta tcactggcat ggttgttccc
     8761 cgtcccctgt ctggcagtat tggaataact ccaggagacg ttccacgaga gacgcttcca
     8821 gctactgctg tagccaaatt gcattaatcg ttcgacatca tcggtatgcc agtattgttg
     8881 ccggcttaat gtcaggtata aattgccata atctctcccc aacgactgcg ttagcgtccc
     8941 ctccacgcaa ctacgtcggt tacctgtttc ccaaaagtct tcagggctgt tgcgccgcga
     9001 tgcccactca ttgagggtat agtagccctc ggtggagtaa cgatagccaa ccaagcgcaa
     9061 gctagtgtct gtgtcgtcaa atcgttttga atagagaaag cgatatgact gacccgtttc
     9121 tgtatcatca tggctaaaat tcgcacgagc atgtgtcaca tcgaaagaaa ttgcgcccaa
     9181 agcgccaaga tctttaccga cacccagagc tgcagaacca taattttcag cagcctgcat
     9241 acctccatat aaagtgatcc cgtgggaaaa accatgaagt atttgcgcct gaaggacgtc
     9301 cggtgtaaat ccgtcttcat cgcgcaattc acccacgctg acatcaacat ctgtctgacc
     9361 ttcacgttta agaatcgcta atgaagcata aggttgggtg aatcgttgtt cgctaccgtc
     9421 ttcttctttg atggttacg
//
LOCUS       DAJTXQ010000146         9432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000146 DAJTXQ010000000
VERSION     DAJTXQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.146"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="OO515_003506"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="OO515_003506"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6577342.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="OO515_003507"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="OO515_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6577343.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="OO515_003508"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="OO515_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OO515_003509"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OO515_003509"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6577344.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="OO515_003510"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="OO515_003510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6577345.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="OO515_003511"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="OO515_003511"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6577346.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="OO515_003512"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="OO515_003512"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6577347.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="OO515_003513"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="OO515_003513"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6577348.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="OO515_003514"
     CDS             complement(6556..7449)
                     /locus_tag="OO515_003514"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6577349.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="OO515_003515"
     CDS             complement(7446..8099)
                     /locus_tag="OO515_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6577350.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="OO515_003516"
     CDS             complement(8110..9369)
                     /locus_tag="OO515_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6577351.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAJTXQ010000147         9220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000147 DAJTXQ010000000
VERSION     DAJTXQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.147"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..732
                     /locus_tag="OO515_003517"
     CDS             73..732
                     /locus_tag="OO515_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6577352.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="OO515_003518"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="OO515_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6577353.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="OO515_003519"
     CDS             complement(1860..2570)
                     /locus_tag="OO515_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6577354.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="OO515_003520"
     CDS             3004..3294
                     /locus_tag="OO515_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6577355.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="OO515_003521"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="OO515_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6577356.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OO515_003522"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="OO515_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6577357.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OO515_003523"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="OO515_003523"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6577358.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OO515_003524"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="OO515_003524"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6577359.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="OO515_003525"
     CDS             complement(7538..7837)
                     /locus_tag="OO515_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6577360.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="OO515_003526"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="OO515_003526"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6577361.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9220)
                     /locus_tag="OO515_003527"
     CDS             complement(9049..>9220)
                     /locus_tag="OO515_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577362.1"
                     /translation="LMVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPP
                     KETQVTQEEYSE"
BASE COUNT         2288 a         2331 c         2269 g         2332 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc taccatcagt
//
LOCUS       DAJTXQ010000148         9143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000148 DAJTXQ010000000
VERSION     DAJTXQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.148"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..872)
                     /locus_tag="OO515_003528"
     CDS             complement(<1..872)
                     /locus_tag="OO515_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6577363.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRER"
     gene            complement(869..2665)
                     /locus_tag="OO515_003529"
     CDS             complement(869..2665)
                     /locus_tag="OO515_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6577364.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(2916..3272)
                     /locus_tag="OO515_003530"
     CDS             complement(2916..3272)
                     /locus_tag="OO515_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577365.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3330..3752)
                     /locus_tag="OO515_003531"
     CDS             complement(3330..3752)
                     /locus_tag="OO515_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6577366.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(3768..4493)
                     /locus_tag="OO515_003532"
     CDS             complement(3768..4493)
                     /locus_tag="OO515_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6577367.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4515..5261)
                     /locus_tag="OO515_003533"
     CDS             complement(4515..5261)
                     /locus_tag="OO515_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6577368.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5268..6332)
                     /locus_tag="OO515_003534"
     CDS             complement(5268..6332)
                     /locus_tag="OO515_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6577369.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6404..6829)
                     /locus_tag="OO515_003535"
     CDS             complement(6404..6829)
                     /locus_tag="OO515_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6577370.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(6826..7089)
                     /locus_tag="OO515_003536"
     CDS             complement(6826..7089)
                     /locus_tag="OO515_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6577371.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7201..7701
                     /locus_tag="OO515_003537"
     CDS             7201..7701
                     /locus_tag="OO515_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6577372.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            7721..7915
                     /locus_tag="OO515_003538"
     CDS             7721..7915
                     /locus_tag="OO515_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6577373.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            7915..8205
                     /locus_tag="OO515_003539"
     CDS             7915..8205
                     /locus_tag="OO515_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6577374.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8486..8638
                     /locus_tag="OO515_003540"
     CDS             8486..8638
                     /locus_tag="OO515_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6577375.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8638..9015
                     /locus_tag="OO515_003541"
     CDS             8638..9015
                     /locus_tag="OO515_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577376.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2343 a         2408 c         1945 g         2447 t
ORIGIN      
        1 cgttccctgt ttataggccc atctgtcgca actacttcag tgaaaactac aagcatgtcc
       61 gaacccgttc ggtcctctcc caaatcaatc aaaataatat ctggaagtgc ttttgaggca
      121 tcaatattca gccccaatgc ttttgccaat acatcatcct gagcaacaac tttattcccg
      181 gattcagaca accagagcac agttggattc ttaagaaaat taggagcaaa aacttcgatg
      241 acggccttag ctattgtact tgacgggccc ggagctaaag ttctcttctc tccatttggg
      301 aaagtgacga ctacggcatc agcagcaatc actgcccctg atttcaataa acgcaaccta
      361 gcaagagcgg ctttgttcaa atgattttct tgccaggaac ttattgcact cagcagcgaa
      421 tctccttcaa gcccggcatc aaagagtgct gcaaaagagc gattcaaact gtaacgagga
      481 cgagaggatg tggtgggtat acctttacgc tcaacaattg cattgcaagg tattaggcca
      541 ttttttatag tttcatcacg tattggctca cgcgtatttt ccgcatacca tgcatcagca
      601 ggccttatct tttgtttaga gaaagtcatc tttacccagc tttctctgga cgcatcatca
      661 gtaagagcag cctgcgaatc tcccatactt acaacctggc tgggccttat ccatcgcccc
      721 agcccctcaa ccgcaccagc ataaaacatg acaaaaattg tttttgcagc tatttcccga
      781 attaaatatc cacgattctc agtcccttca gggaaaatta attgtaatct ttcccggata
      841 acattcaatg ggggaacaac aggcagattc atgcaatcat cactccatag aaaccagcga
      901 tagttctctc aataatccgt ctgtggacgc cagcatcaat taacgattga atctccatca
      961 cctgctccag agaaggcaac gggattgaat tcaattcata ggccgaaaca gcaacacttc
     1021 cgcttataca cctgaacgcc cgatcgacaa catgcgaatt aagtagcata tcaataacat
     1081 cagggcgaac cgcagagaac aatccgtttg aatatacaat attaatatgg ttttcaacta
     1141 ctacgccacc agtttcatct ataaatccct gaggtaaaat tgccgctaaa atccttctgt
     1201 cctgttcctt cgatgttgtc cgctgaacaa gcacacattc agattttgtc acaaggaaac
     1261 cctgttgtgg ggttatatcg atatagggaa catgattttt tctgtctgca ctaaaacgaa
     1321 aaccagctga agttattgat tccgcccaga ctaatgggta gctatttttt cctttcgtcg
     1381 tacgcaactg cgacttaaat cgattccaga ctaactgacc tgtagagact gagtatccca
     1441 gatcagctaa acgcgtagac atctgtttta atacattaag aaaacaggca tcatctttaa
     1501 cgcgtggcaa aagccacgtt gctccacctt tttctatatc aacattacct attttctcaa
     1561 tttttgcttt gttcaacccc ttagggacaa gtgaggatac ctgggcccga actttatgtt
     1621 ctcccgcctt gaatgctgtc aacattgttt cctggagaac atcgtcaaag accccatccc
     1681 tgtcagtgac gaaatcaagg gcatacggag tagttttttc ggtcaaaagg gttcgcagcg
     1741 cggtgaaata ctgcccacca agaaaagaag ttggcgtaag aaatgcaata actcctgttt
     1801 tctctttaac catgcgaaca gctaagtcgg tgaacaatcc atatagattt gcatgaccaa
     1861 ataacgaacg agaatatttt tctctcgttt taatatcgag agtgacacgt ccgtaaggcg
     1921 gattccctac cacaagatca taaccagaaa atttatcttg ctgaagcgca tcagcaacaa
     1981 taattgtatc ttcaggcaat ctacgtttta cctttacaca tagtggcatt agtacagatt
     2041 cgagcaatac caaactcatc caagccgcaa atggatcgat ctctatcccc tttaaacgac
     2101 gactgattct cctcaacatc cattcagggg aagacccctt gtcttttttt aacattcgaa
     2161 tggctactgg tgccaaaaat gcaccgccac cacacgcagg atcaataacc gatgcatgag
     2221 aaaaatcaac accagacttt tcagccaggt ccaagagacg cgctaccaaa ggtggggggg
     2281 tatagtaagc ccccaattcc gaacggtaag cggatggcag cattaccgta taaattgacc
     2341 caatcagata tccagcgtcc tccgccggaa attgagcaat aagctcacct gttcgttcag
     2401 cgagctcgcg tgcatcacat ggaatgtctt ctatgtaaac agtgtgcggt actgccctca
     2461 ttttaatgct gcttttttgc ttttttgcaa gggtatccca atatgcagct acgatgctga
     2521 agcagtatga gcgggcatgc atcaaccttg actgcccggt tttgtaattg cccgcataac
     2581 ctttggccat ggctttacaa gtttgatatc tttccagatt agaaaactca ttatctgtaa
     2641 aaaaatcaaa atgttgcaca ttcataatgt aaaatcctaa tgtatcaaac atgcgggcca
     2701 taacatctca tggtggcgca tagcaaactg gcccgaagaa tatcacgcac caagtcgatc
     2761 tcataacccg ttatgacaat aaatctttgt agctgaactc acttggtgat ttgtcaatca
     2821 ttcctggcct ccagttcgtc ctgaatctct ttatcgagtt caccgtattt gttagtcctc
     2881 atcactcacg acttgacttc cagaaataac cttgattatt cggggctata ttttgtcacc
     2941 ctgcgtatcc gcgctttcgc attacgctca atctggatta gcttttctat attttttcgc
     3001 ctttcccgct cctcctggcg caagagcctt acatcatctg ccagtctggt ttctcttttc
     3061 gccacagaga gcatccagtc aaatggctcc acaactgcac cgcaaatttt acagcggacc
     3121 tgacgctctt tttcgtcaac ccggacagaa gcgtgatggc aatatggtct ttccgatggc
     3181 tcataaagaa aattaacctg attacgaggg ttatcctctt ttaccggaaa taaaacaata
     3241 ttgcttaact catcttctgg ttttatttcc atgctcctct cctttgatgc gaatgccagc
     3301 ggtaattgaa gcctgatagc taatttcact cacagtaccg cctcctgaaa atcaccctga
     3361 tagaaagccg gtacacgctg cataacttca ctcttccggc actcgcgaca gattatgttc
     3421 tgatgtctgt cgtagcggcg tatttctccg tccggtaatg accagataag gtccggatca
     3481 accacagatg gtttcttcag ctttgccctc gagagttttt tgcgggcgtt ttgccagtcc
     3541 ttacgagcct gttcagacgg gaataacccg tagccagagt tgtatacatc cccactggca
     3601 accagctctc tggcgagaac gcttattaaa tatcttgtcg ccccggtttt agcttccagt
     3661 tgtcgtaacg tctcgcgccc actctggcgt acgagttcaa caacctgccc cttaattttt
     3721 tcccgctctt cctgtgtaaa tacttttgcc ataagcgccc ccggcaatca cttttccgac
     3781 acaatacgac cggaggaatc gacaatctgt cgaacaatat cccggtgctt gttcagctcc
     3841 cgcaacgcgg cgcagacacg ctcccacttc tggacatgac ttttcgcccg acgcagttcg
     3901 cggtttgcca tatgcagcga tggtaaaacc aggtcatccg cttgcgtttc ggtgaacgat
     3961 ggcaacgact gcacaatgtc cgccacagtt tctgttttaa tttcttcctg tgttgcagct
     4021 tcctgtaccg gtaacgcaac acctgctggc tgaggaaaga ctttaccatc cgtttccgct
     4081 atggatgcgg ctttcggctc tgccggtaaa tcagcgcccg gtatgcagta ccgaaattta
     4141 ccgccctgat tcacgcgaat cagacgccct ttgctgattg ccatggccag cgatgaattc
     4201 gcccggcggg aggtaatccc gaacatcagt gccagctcat ccgccgtttg tgggccatgt
     4261 tgttcaatcg cctcagtcag catttgcgct gtcactttcg gtaccggtga cactggttca
     4321 ctttcaccag cctgaatcag ccaccacatc gaacccttgt tatccgcttc accacggcgc
     4381 ttcagtttcc acagttcgtt gaccgcatct tcacggctga ttccaaggcg ggacgccacc
     4441 acatgtgaag aggctctttt cagtgctttc agtgcgtcaa atacagtttc cattaaaaca
     4501 tcctccaaca aaaattactt cacaactccc ggaaagctga catttgaacg ccagctatcc
     4561 cagttaaacg tcacccaccg acaaccgttc atggtcatgc ggtccataat cctctcacca
     4621 agaagcgtgc tcattgcggc atgattcagg tttgttaaca tcccgacact gcacagtgat
     4681 gctgtccggc gatcaattat ctggtgcaat accacctgct cgtttttcgt ctcccgctga
     4741 atgcctattt catccaggac cagcaaatca accccgcaaa gctcctgtaa aaatttttca
     4801 ccggatttgc cgttgtcgta gctgtcatgc aacacgctca tgacatcaga ctcggtgacg
     4861 ataatcacgc tgcgcccctt cgccatcagc cggttaccca tcgccgctgc aaggtgattt
     4921 ttcccggtgc cggttttacc gctgaacaca aaattcgtgc acccggtcat cagttcgtca
     4981 gctatggatt tggcctggct cagcgcgtat ttttgcccgt cgttctgcac ctgataattc
     5041 gcaaacgagc atttgctgtg cagaggctgg atgcccgaac gattcaggat tttttccacc
     5101 cgcaactggc gattctggcg gttaatctcc tcgctgcgtt ttcgtccttc agcaagttgc
     5161 cattcccgcc actcctccac cgtccggtac ggtggaaccg ccccctgtgg tgcaagtctg
     5221 tgaatacgtt caagaacccc ggctgccgca atgtttttca tgccacatca ccccctgaat
     5281 cccggcggaa tttcggtatc cggttcagaa atatgattca cacaacgctg gttgttcgtg
     5341 ccgcttaccg ggagcaacca ggggttttca aaattccggt ccggcccaaa aaacgtcgtc
     5401 gctcgctgaa caaattccgt tcccgctttc ccggtcgccg ccaggtatct cgcgtaacgc
     5461 ctcacaccat ccagcatggt ctctggtggc accccctcgc gcaatctggc cttccaggca
     5521 ctgaatgcgg atttcttcgg gtttgccccg gcacgcaacg ggtattcccg ccagacctgt
     5581 tcgaacacat ccggataatc cactcgtccc acaggctgcc cggtgttttc cggggctacc
     5641 cgatcggctt cccgctgaat ggcggaatcg gcttcaggct gctgcagttg gagtgattgc
     5701 tccggccttg cggtcatcac ctgctgcaca gcgcccgaat cggctttcag cgcatacgct
     5761 gaatcggctt ccggtgtcga gcctgctggc tgaccaggat tgacggtctg aacatcccct
     5821 gcctggttcg tggcgttttt tacgccatgg accatagtgt tttgatcttc tttatctgta
     5881 tctttatctg tatctgtcgt gactcgtcgt gacatgtgcg tgacatttcg tgacgcgccg
     5941 tgacaatcgc cattttgttc ccgctttctt tccctctctc gctgcgccct cttgcgctct
     6001 gcaggagatt ttgcggtttg cgaaatattg ccgttgtcct ctttaagcac ctggcgtttt
     6061 tcccatccag tgattaaatc accatcaagt acccgcccct gcatcgtctg caaaattgaa
     6121 tcaattacct cttctgtcac gtcgagcgca cttgccaaat cttctgtcgt gacatcaatg
     6181 tgacctcgcg tgacatttcg tgacgcgctc accaggaggt ggatatacac tgccatcact
     6241 gttgcaattg gctgccctga caccctggca attgttcgcc acttagggtc atttggcatg
     6301 tcatgccata atctgagcca ggcgttagcc atactcacct cttctgatac cgaatctttt
     6361 tactcacgag ttgccgggag cgattcgata tggctattgt cagtcaatgt actgccacag
     6421 catttcctgc cgggccacca cggttcatct gattgaaacc ggcgattgcc actgcgacaa
     6481 aatcatcagc gtctctcacc agtcgttcat gcgtctccac cagttcccga aaataagctg
     6541 aactgtggct gcgcattcga gccaccagcg gaggtggcat tgccctttcg atcgcaggta
     6601 acaacgcctg aattttttca acagcgtcag gggtgtcttt atccagccaa cggaaaatct
     6661 tttgggtgtt gagataaagc gtgttgtgct tgctgtcgtt atgcagaacc ggaaacgtca
     6721 ttcccagttc gaaataaacc ctggttattt cagctgccgg aactttttca ccgtccggat
     6781 gcgcccaggc tttcatcgcc atgcggatgt gttcatgctt gattttcatg aatcaacccc
     6841 cgccgatggt tgtgtgttag cctgaaactc agcaggcaag ccgtcagttg ggttggggta
     6901 ggttttactg tcaatctcat gtggggtcac cacccatccc gttgcttcac accaacgtaa
     6961 tattttttct cctgtaagtt ttgcacgccc cgttataacg tggctgacca tcccttggga
     7021 tgcgccaaca atctcagcaa atgaactctg ggttatatga aaatgatgca aatattctcc
     7081 aagattcata atccgcccta tgtgatgtgt taccaatgca taattaatag cactgttatt
     7141 tttaaaagta aatagtgata ctatttcaaa gtgattagca atcttattag aattggatgt
     7201 atgaaaagaa aatcattgtc agacaccgac ctcaacgccg ccagaagact gaaagaaatc
     7261 tggatggcaa agaaatctca gttgggattg actcaggaac gtgcggcgga aattatgggg
     7321 ttttcaacac aaggggcggt aagtcactac ctaaatggtc aaaccccact aaaccttgag
     7381 gctgttctaa agtttgctgg cttactgaaa gttccccctg agtcaatcag gccagacatg
     7441 gtagatttgc tgcaaattgc aaggatgtat tgtcgagaac cacagcaaga taacatagtc
     7501 gcattaccag cagacacaga acaaacagag gatgaactac cgtttgccgt agagccgatg
     7561 gaacgagatt taatccatac gttcagagct tttccgaaag aagatcaaga acacatgctt
     7621 caggagatga aagataaaaa agaatcaatg gatcgtacgg ttgcgcggtg gttagctgca
     7681 caaaaaggcc gtcgcgccta aatcagagga gatatgtaaa atgagtacag tcctttcccc
     7741 aatggtttca gaatttgaga cagttgagca agaaaatagt tacaacgaat ggctgcgggc
     7801 caaagtagca gcaagcctcg cggatccccg cccggcgatt ccgcatgatg aggtaatggc
     7861 ggaaatggaa caaattattg cccaaattgc tgctgcaact cacagaggtg aataatgtta
     7921 cccgttttat ggctaccatc tgctcgtgat gatttgcgcc agatcgtagc ctacatagct
     7981 aaagaaaatc ccgcggcagc acgcagaatg aaaattcgaa ttgaaacatc cgttttgccg
     8041 ctcacagagc atccttacct gtatcctcca agcgagcgag ttcttggtct tcgtgaaatt
     8101 gtaactcatc ctaactacat catcctttac cgagtaacag catcaagcat agaagtagta
     8161 aatattgtgc attctcgaag acagtatcca ggcaaaaaca gttaattatt cccgccgaca
     8221 gaaccacctt agggtggttt tttcttgccc caacaaaaaa caatagcgat gctatttaca
     8281 tcaaaaaaaa ataatgatgc tattgtttca ggcgtcaacc caccccgccc cacagaatgc
     8341 agggcaatac ttcgagttac cagacagtgg tcaggggtta agtagccagc ccgaggcgta
     8401 agaacatgac ggcagggttc aatctttaac tatgcagcag gttttttgtt ccgccacccc
     8461 ggcgttaagg ggaaatgagg tcagcatgga tactatcgat cttggcaaca acgaatctct
     8521 ggtgtgcggc gtgttcccta accaggatgg aacgtttacc gccatgacgt ataccaaaag
     8581 caaaacgttt aaaaccgaat ctggcgcgcg ttgctggtta gccagaaaca ctgactgatg
     8641 aggctgacga tggaatttaa agatttaccg tctgatgtac agaaaacagc agctcataca
     8701 ttgcattctg tgctgcgaga aatcgggaaa gatattgcaa gcgagccagc aaaagatctg
     8761 gcccggaaaa tcaagaccgc tttcgttgag ctttataatg ttggcactga ctctgaaact
     8821 gtcgagacca agaccgtaag ttcaccaata ttctcacttg agccaagcgt attatcaggt
     8881 gaaatatgca ccgagatttc cagcgaactg cttccggtaa tacgagaagc aattcgccgt
     8941 cgcggtttgg atggaagtta tgatcatgac gttctgcaag ttctcaggac aatggtgact
     9001 tcactgggga tttgatccct gcgtcctcta agcttttgat atagcggtcc tccaagaagc
     9061 ggtaaatttt gtcgaaatca tcttttccct ctggtttatt cagaggatta caggacccgt
     9121 aagtagcggc atacatttca acc
//
LOCUS       DAJTXQ010000149         9074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000149 DAJTXQ010000000
VERSION     DAJTXQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.149"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OO515_003542"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OO515_003542"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6577377.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="OO515_003543"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="OO515_003543"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6577378.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="OO515_003544"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="OO515_003544"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6577379.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="OO515_003545"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="OO515_003545"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6577380.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="OO515_003546"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="OO515_003546"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6577381.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5059..6192
                     /locus_tag="OO515_003547"
     CDS             5059..6192
                     /locus_tag="OO515_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6577382.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="OO515_003548"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="OO515_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6577383.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OO515_003549"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OO515_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6577384.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="OO515_003550"
     CDS             complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="OO515_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6577385.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="OO515_003551"
     CDS             complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="OO515_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6577386.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7737..8303)
                     /gene="efp"
                     /locus_tag="OO515_003552"
     CDS             complement(7737..8303)
                     /gene="efp"
                     /locus_tag="OO515_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6577387.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8345..>9074
                     /locus_tag="OO515_003553"
     CDS             8345..>9074
                     /locus_tag="OO515_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HCR6577388.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKL"
BASE COUNT         2296 a         2346 c         2323 g         2109 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacccggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac gttactcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaaccg gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcacgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg
     2221 cacgctggtg cagatccgtg ctaacgcggt cgttatggct actggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaat gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctagagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggt ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggcgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tatcgttaca atctaacgta tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttactgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 accccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg
     6361 cgtgacagta gtgaataatt tcgacgctaa acgctatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagtctgcg
     6481 tgatgacggc ggcctgaatg tcatcaataa aggctataac cctgacagag aaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgtcattgca cttgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacagtaata acacttggcg tcaaacgact aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 agaattgggg ccatccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attacctttg tgactatcgc
     7261 aaaaaaacaa acggcacgac ataactgtcg taccgttatt gcttattgct gcgctttcgt
     7321 tgctgcacca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     7381 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     7441 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     7501 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat
     7561 tagctggcag cgcgggagat ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga
     7621 gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc
     7681 atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac
     7741 ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt
     7801 acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta
     7861 cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc
     7921 ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc
     7981 agcagccatt tagcgttgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt
     8041 tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg
     8101 acaacatcag cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg
     8161 gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat
     8221 tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa
     8281 tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca
     8341 caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact
     8401 tgccgatgtt gtgaccgatc ctggtgaact tctgcgtctt ttgaatatag acgcggacga
     8461 aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt
     8521 tatcgatcgc atggagaaag gcaatccgga cgatcctatt ttgcgtcagg tacttacctc
     8581 gcaagatgag tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag
     8641 cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg
     8701 ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg
     8761 caacaagcgt aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga
     8821 gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg actggttgct
     8881 cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat
     8941 tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca
     9001 aatcttgctg gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc
     9061 gatggctaaa ttgc
//
LOCUS       DAJTXQ010000150         9072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000150 DAJTXQ010000000
VERSION     DAJTXQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.150"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="OO515_003554"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="OO515_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6577389.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="OO515_003555"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="OO515_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6577390.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="OO515_003556"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="OO515_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6577391.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLCLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="OO515_003557"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="OO515_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6577392.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="OO515_003558"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="OO515_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6577393.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="OO515_003559"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="OO515_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6577394.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="OO515_003560"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="OO515_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6577395.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8343..9040
                     /locus_tag="OO515_003561"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="OO515_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1988 a         2507 c         2584 g         1993 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac atagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgctccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAJTXQ010000151         9049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000151 DAJTXQ010000000
VERSION     DAJTXQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.151"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(350..1603)
                     /locus_tag="OO515_003562"
     CDS             complement(350..1603)
                     /locus_tag="OO515_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6577396.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OO515_003563"
     CDS             1710..2603
                     /locus_tag="OO515_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577397.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OO515_003564"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OO515_003564"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6577398.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OO515_003565"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OO515_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6577399.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO515_003566"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO515_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6577400.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALSASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO515_003567"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO515_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6577401.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OO515_003568"
     CDS             complement(6839..7693)
                     /locus_tag="OO515_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6577402.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO515_003569"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO515_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6577403.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9049)
                     /locus_tag="OO515_003570"
     CDS             complement(8323..>9049)
                     /locus_tag="OO515_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR6577404.1"
                     /translation="GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2367 a         2298 c         2351 g         2033 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacaggtaca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcgga aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtcct
//
LOCUS       DAJTXQ010000152         8920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000152 DAJTXQ010000000
VERSION     DAJTXQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.152"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..154)
                     /locus_tag="OO515_003571"
     CDS             complement(<1..154)
                     /locus_tag="OO515_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6577405.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLA"
     gene            complement(154..1080)
                     /gene="glsB"
                     /locus_tag="OO515_003572"
     CDS             complement(154..1080)
                     /gene="glsB"
                     /locus_tag="OO515_003572"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6577406.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1144..2532)
                     /gene="sad"
                     /locus_tag="OO515_003573"
     CDS             complement(1144..2532)
                     /gene="sad"
                     /locus_tag="OO515_003573"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR6577407.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2633..3514
                     /locus_tag="OO515_003574"
     CDS             2633..3514
                     /locus_tag="OO515_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6577408.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3591..4706
                     /gene="yneK"
                     /locus_tag="OO515_003575"
     CDS             3591..4706
                     /gene="yneK"
                     /locus_tag="OO515_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6577409.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4856..6046
                     /gene="ydeA"
                     /locus_tag="OO515_003576"
     CDS             4856..6046
                     /gene="ydeA"
                     /locus_tag="OO515_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6577410.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6071..6736)
                     /gene="marC"
                     /locus_tag="OO515_003577"
     CDS             complement(6071..6736)
                     /gene="marC"
                     /locus_tag="OO515_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6577411.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6948..7382
                     /gene="marR"
                     /locus_tag="OO515_003578"
     CDS             6948..7382
                     /gene="marR"
                     /locus_tag="OO515_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6577412.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7402..7785
                     /gene="marA"
                     /locus_tag="OO515_003579"
     CDS             7402..7785
                     /gene="marA"
                     /locus_tag="OO515_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6577413.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7817..8035
                     /gene="marB"
                     /locus_tag="OO515_003580"
     CDS             7817..8035
                     /gene="marB"
                     /locus_tag="OO515_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6577414.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8092..>8920)
                     /locus_tag="OO515_003581"
     CDS             complement(8092..>8920)
                     /locus_tag="OO515_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6577415.1"
                     /translation="EREQIMYQAVHYELVASALAVQTGKSINPEFNIGCMIAMCPIYP
                     LTCAPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILASG
                     CVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLNW
                     FWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLIGY
                     TPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2282 a         2286 c         2046 g         2306 t
ORIGIN      
        1 gagcaagacg tttagcgaca aaatccgccg catgttgttc gatgactggc gcgcctttct
       61 ccaggcaata ctgacgctct ttcatgccca gacgaaagcg agagcgaaat tttgaacggg
      121 ataaacgcgc gaagagtgga tcgagagact gcattaataa accgaacgcc ctaactgttt
      181 cgtcaattgt tcaaggacgg cgatacccgc aagcgagtta cctgcatcat ccagttccgg
      241 actccagact gcgatggcca tttcatgcgg aacaatcgcc acaataccgc caccaacgcc
      301 agatttcgcc ggtagtccca cccgccaggc aaactccccc gcgttctggt acataccact
      361 ggtcgccatc agcgcgttaa tttgccgcgc ctgcattggc gtcactacag gttcatcaat
      421 atgaatagct ttcccctgat tagccagaaa gacaaacgtc cgagccagct ctacacagct
      481 cattttcaga gcgcagtaat gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa
      541 attgccaaac gacttcatca gccaggcgat agccgcattt cgcgcggaat gttcaaattc
      601 ggaacgcgct accaccgtat cgtatgaaat atcagacaca ccgcttaagc cgcgcacgac
      661 ttccagcata cgttgccgtg gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag
      721 cgcaccagca ttaataaacg gattacgcgg aataccctgt tccatttcca gttgcactaa
      781 agaattgaac ggtgatccag acggatcttt gccgacgcgt tgccagattt cctcttcgga
      841 gtaatgacgc atggcgacaa cgagactcag cactttggaa atagactgaa tggaaaaacg
      901 ttcttgcgcg tctccggcct gaaaaagctg tccgtcaacg gtacagatag caatgcccaa
      961 tcgggaaccg tctactgtag ccagcgccgg aatataatcc gcgactttac cctgaccaat
     1021 gagcggccgc acttgccgca agatgttttc taaaattgca ttatccatgg cgactgccac
     1081 tttctactcc tggaccgcag gtctgaaaag acctgcgagt atatcagagc tgaatatgtc
     1141 gcgtcagatc cggtctttcc acaccgtctg gatattacag aattcgtgta agccgaaatg
     1201 ggaaagctca cgaccaaagc cactcttttt cacgccacca aaggccactc gcgcgtcgct
     1261 ggcacaataa ccattgataa acaccccacc gcattccaga cgtgccgcca tctgtctggc
     1321 ctgtgtttcg tcggtggtaa aaatggtcgc tgaaaggccg aactcactat cattagccag
     1381 ttccagcgca tgttctgcat ctttcgcaac ggtgattgcc gcaacggggc caaacatttc
     1441 ttcccgaaac gcggtcattt ctggggtaac attcgccaga accgttggcg gatagtagtt
     1501 acctgcccca gccatctttt ccccgcccag taacaaacgc gcaccctgcg ccagggtttt
     1561 ctctacctga tgatgcagct catcacgtaa atcaaaacga gccattggtc cgagagtgtt
     1621 ctcttcgtca cggggatcgc ccattttcaa ggctgccgca gctgccacaa aacgttcggt
     1681 aaatgcagaa gcaattccct cttcgataat aaagcgtttt gccgctgcac atacctgtcc
     1741 ggtattctga taacgtccgg ctaccgccgc tttaaccgcc agttccagat cggcatcgtt
     1801 aagcacaata aacggatccg aaccgcccag ttccagtacg cattttttca gtgccgctcc
     1861 agcctgtgcg ccaatagccg ctccagcacg aacacttccg gtcaccgtga cagcagcaat
     1921 gcgcgagtct ttaatcatct gactgacacc gtcgttgtcg gcattcagcc agccatatac
     1981 gccttgcggg attcccgcat ctttaaacac ctgggcaatg agctgagcac atcccatcac
     2041 attcggcgca tgtttaagta agtagccgtt acctgcaaga atgatgggaa cagcgccacg
     2101 catcacctgc cataacggaa aattccacgg cataatcgcc agaatcgtcc ccaacggtcg
     2161 atactcaata accgcctgct gattttccac cagtgtaggt tccgccttca gcattgccgg
     2221 accatgttct gcataccagt cacacaaatt cgccgatttc gccacttcag cgcgcgcctg
     2281 gttgattggt ttgcccattt cgcgggtgat catttgcgcc atttcttcgc tacgggcgcg
     2341 cagagcctta ccgatatcac gcattttttc agcacgataa tctatatttg tctcgcgcca
     2401 gtcgcgaaag cctgctgccg ccagctcaag tgcgttttcg atatcgttag cgccagccca
     2461 cggcagcaca gaaagttgtt cacccgtggc aggatttatt gaaattgcat gagttgccgg
     2521 agtaatggtc atcggggtat ctcctttatg agtcatggta tgaagatacg cagatttact
     2581 cttgctttaa aatgaataat attaagccac ttattcacga atcgagaatg ttatggatct
     2641 gacccaactg gagatgttca acgcggttgc cgaagctggc agcattaccc aggctgctgc
     2701 aaaagtgcat cgcgtaccgt cgaatctcac tacccgtcta cgccagctgg aaacagaact
     2761 gggggtagat ctgtttattc gcgagaatca gcgtttacgt ctctctcctg ccgggcataa
     2821 ctttttacgc tatagccaac aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc
     2881 tggcgatgag ccgcaaggtt tattttctct tggttcgctg gaaagcaccg cagcagtgcg
     2941 cattccggcc acgctggcgg aattcaacca tcgttatccc aaaattcagt tttcactttc
     3001 caccggccct tccggcacta tgctggaagg agtgctggag ggaaaactga atgcggcatt
     3061 tattgatggg cccattaacc atactgccat tgacgggata ccggtatacc gcgaggagct
     3121 gatgatcgtc acgccacaag gtcatgcgcc agtaatccgc gccagtcagg ttaacggcag
     3181 taacatttat gccttccgca ccaattgttc gtatcgtcgc cacttcgaga gctggtttca
     3241 tgctgacggt gccgctccgg gaactattca cgagatggag tcgtatcacg ggatgttggc
     3301 ctgtgtggtc gcaggagcag gcattgcgct tattccacgc tctatgctgg aaagtatgcc
     3361 ggggcatcac caggttgaag cgtggccgtt agctgagcaa tggcgttggt taacaacctg
     3421 gctggtctgg cgtcgcggtg cgaaaacgcg tccgcttgag gcatttattc aactactgga
     3481 tgtgtctgac tcggcaaaac agagttacca atgaactatt ttgataattc ttgcggctaa
     3541 tatgctctat atagtgatgt tccgatgact tatgactata tggggcaaat atggttacgc
     3601 cagtaagcat cagcaattac atatctcttc ctgatgattt tcccgttcgt aatattgcac
     3661 cacaggtaaa agaagtttta aaggacttta ttgatgcgct aagtacaata atatgtgatg
     3721 aagagtggcg cacgtcttta aacatcaact cagccacaaa aaagatattt aataaccttg
     3781 ataacttatc atatattcag aggacttctt tccggggtaa cgacacgcta tacaatgaaa
     3841 aggttcagtt taaactcact tatcccgtca agaatggaag acacaaagaa aatattgaat
     3901 ttcaggtagt aataaattta agccctattt atttagataa ttttcgccat gatggagaaa
     3961 ttaatatttt ttgcgctccc aacccaaagc ctgtcactat ggggcgcgta tttcagaccg
     4021 gcgtcgagcg tgtgctcttt ctgtttctga atgattttat tgaacaattt ccaatgttca
     4081 accctggcgt tccgatcaaa atagcgcata caccacatat tgaacccctg cctccggatc
     4141 accataccgc tgcagattac ttacgccagt ttgatttgct tgtcctgaat tttatctctc
     4201 gcggtaattt tgtcatactc ccccgattat ggaataactc tgaggttcac agatggttcg
     4261 tcaataaaga tcctaacttg atcaccgcca ttctcgacat aacggacagc gaattaaaag
     4321 aggatttatt gcaaagcctg atggattcat taggttctaa caaacatgta ctacccgaag
     4381 tctgcatctg ctttttattc cttttagcag aacaagaatc tcctcatttt caggacttgt
     4441 ttttattttt cgccaatatg ttattgcact atcaccaatt tatgaatccc aatgaaagtg
     4501 atttgaatga cgtgttgatg ccagcatcat taagtgatga taaaattatc aagcatatgg
     4561 cgcgcaggac cctcgaactg tttgtaaaaa atgaaacacc gccaaaagtt actcacgaag
     4621 acctggtgaa aaacaggcct cgctcccctg tcagaccacc tatacccgca acctccaaaa
     4681 cgccagacct ccctgaacgt cattaaaccg tgatgttacc gactctctga cgcggaacag
     4741 aatcagcgtc agagaaacgg aaaacgcgat ccagatcaca aatacattgt attcacatca
     4801 ttaaccgttt taagatcatt tcatcacttt ttcgcaactc acccgataat ctgttatgac
     4861 aacaaacact gtttcccgca aagtggcgtg gctacgggtc gttacgctgg cagtcgccgc
     4921 cttcatcttc aacaccaccg aatttgtccc tgttggcctg ctctctgaca ttgcgcaaag
     4981 ttttcacatg caaaccgctc aggtcggcat catgttgacc atttacgcat gggtagtagc
     5041 gctaatgtca ttgcctttta tgttaatgac cagtcaggtt gaacggcgca aattactgat
     5101 ctgcctgttt gtggtgttta ttgccagcca cgtactgtcg tttttatcgt ggagctttac
     5161 cgttctggtg atcagtcgca ttggtgtggc ttttgcacat gcgattttct ggtcgattac
     5221 ggcgtctctg gcgatccgta tggctccggc cgggaagcgg gcacaggcct tgagtttaat
     5281 tgccaccggt acagcactgg cgatggtctt aggtttacct ctcgggcgca ttgtggggca
     5341 gtatttcggt tggcgaatga ccttcttcgc gattggtatt ggggcgctta tcaccctttt
     5401 gtgcctgatt aagttacttc ccttactgcc cagtgagcat tccggttcat tgaaaagcct
     5461 cccgctattg ttccgccgcc cggcattgat gagcatttat ttgttaactg tggtggttgt
     5521 caccgcccat tacacggcat acagctatat cgagcctttt gtgcaaaaca ttgcgggatt
     5581 cagcgccaac tttgccacgg cattactgtt attactcggt ggtgcgggca ttattggcag
     5641 cgtgattttc ggtaaactgg gtaatcagta tgcgtctgcg ttggtgagta cggcgattgc
     5701 gctgttgctg gtgtgtctgg cactgctgct acctgcggcg aacagtgaaa tacacctcgg
     5761 ggtgctgagt attttctggg ggatcgcgat gatgatcatc gggcttggta tgcaggttaa
     5821 agtgctggcg ctggcaccag atgctaccga cgtcgcgatg gcgctattct ccggcatatt
     5881 taatattgga atcggggcgg gtgcgttggt aggtaatcag gtgagtttgc actggtcaat
     5941 gtcgatgatt ggttatgtgg gcgcggtgcc tgcttttgcc gcgttaatat ggtcaatcat
     6001 tatatttcgc cgctggccag tgacactcga agaacagacg caatagttga aaggcccatt
     6061 cgggcctttt ttaatggtac gttttaatga tttccaggat gccgttaata ataaactgca
     6121 cacccataca taccagcagg aatcccatca gacgggagat cgcttcaatg ccacccttgc
     6181 ccaccagccg cataattgcg ccggagctgc gtaggcttcc ccacaaaata accgccacca
     6241 ggaaaaagat cagcggcggc gcaaccatca gtacccaatc agcgaaggtt gaactctgac
     6301 gcactgtgga cgccgagcta ataatcatcg ctatggttcc cggaccagcg gtacttggca
     6361 ttgctagcgg cacaaaggca atattggcac tgggttcatc ttccagctct tccgacttgc
     6421 ttttcgcctc cggtgaatca atcgctttct gttgcggaaa gagcatccga aaaccgataa
     6481 acgcgacgat taagccgcct gcaattcgca gaccgggaat cgaaatgcca aatgtatcca
     6541 tcaccagttg cccggcgtaa tacgccacca tcatgatggc aaatacgtac accgaggcca
     6601 tcaacgactg acgattacgt tcggcactgt tcatgttgcc tgccaggcca agaaatagcg
     6661 cgacagttgt taatgggtta gctaacggca gcaacaccac cagccccagg ccaattgctt
     6721 taaacaaatc taacattggt ggttgttatc ctgtgtatct gggttatcag cgaaaagtat
     6781 aaggggtaaa caaggataaa gtgtcactct ttagctaacc ttgcatcgca ttgaccaaaa
     6841 cttgaaccga tttagcaaaa cgtggcatcg gtcaattcat tcatttgact tatacttgcc
     6901 tgggcaatat tatcctctgc aactaattac ttgccagggc aactaatgtg aaaagtacca
     6961 gcgatctgtt caatgaaatt attccattgg gtcgcttaat ccatatggtt aatcagaaga
     7021 aagatcgtct gcttaacgag tatctgtctc cgctggatat taccgcggca cagtttaagg
     7081 tgctctgctc tatccgctgc gcggcgtgta ttactccggt tgaactgaaa aaagtgttgt
     7141 cggtcgacct gggagcactg acccgtatgc tggatcgcct ggtctgtaaa ggctgggttg
     7201 aaaggttgcc gaacccgaat gataagcgcg gcgtactggt aaaacttacc accagcggcg
     7261 cggcaatatg cgaacaatgc catcaattag ttggccagga cctgcatcaa gaattaacaa
     7321 aaaacctgac ggcggacgaa gttgcaacac ttgagcattt gcttaagaaa gtcctgccgt
     7381 aaacaaaaaa gaggtatgac gatgtccaga cgcaatactg acgctattac cattcatagc
     7441 attttggact ggatcgagga caacctggaa tcgccactgt cactggagaa agtgtcagag
     7501 cgttcgggtt actccaaatg gcacctgcaa cggatgttta aaaaagaaac cggtcattca
     7561 ttaggccaat acatccgcag ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt
     7621 aacgagccga tactctatct ggcagaacga tatggcttcg agtcgcaaca aactctgacc
     7681 cgaaccttca aaaattactt tgatgttccg ccgcataaat accggatgac caatatgcag
     7741 ggcgaatcgc gctttttaca tccattaaat cattacaata gctagttgat aacgtgacaa
     7801 cgtcactgag gcaatcatga aaccactttc atccgcaata gcagctgcgc ttattctctt
     7861 ttccgcgcag ggcgttgcgg aacaaaccac gcagccagtt gttacttctt gtgccaatgt
     7921 cgtggttgtt cccccatcgc aggaacaacc accgtttgat ttaaatcaca tgggtactgg
     7981 cagtgataag tcggatgcgc tcggcgtgcc ctattataat caacacgcta tgtagttatt
     8041 gtcaggctct gatcacagag cctgacatct ccgtaatatc ttcctcaagt ctcaaatatt
     8101 ttcgccattg ttggcgatga gatcccgata ccagtaaaac gacgctttac gtatccgttc
     8161 cagcgttccc ttcccttcgt tgtctttatc gacataaatc attccgtagc gctttttcat
     8221 ttcccctgtt ccggcagaaa ccaggtcaat gcagccccac ggggtgtagc caattaagtc
     8281 aacaccatct tcaactacgg cttttttcat ttcccgaata tgggaagcaa agtaatcaat
     8341 gcgatagtga tcgttcaccg tgccgtcagc ttgtctctgg tcaaccgcac caaatccatt
     8401 ttcgacaata aacagcggca actggaaatg atcccagaac cagttgagtg aataacgtag
     8461 ccctgccgga tcaatttgcc atccccattc ggatgtatcg atataagggt tgctgaccaa
     8521 atctcgtggt tcaacataat ccagttgcgg gttatcggga gaaaattgtg tcgtaaaaga
     8581 catgtagtag ctaaaaccga taaagtcgac acatccactg gcaagaatcg cgttatcttc
     8641 tggtgtgata tcgaggttga atcctttcct ggcaaagtaa ttcagcatat gttgcggata
     8701 atatccacga gcatgaacat cagtaaacca gtaacgacga tgcatcgctt tcgtggccat
     8761 catcatatcg ttgggtgcac acgtcagagg atagatgggg cacatggcga tcatacagcc
     8821 aatgttaaat tcaggattga tcgattttcc agtctgtacc gccagggcac tggcaactaa
     8881 ctcgtaatgt accgcctggt acattatttg ttcgcgctca
//
LOCUS       DAJTXQ010000153         8838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000153 DAJTXQ010000000
VERSION     DAJTXQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.153"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            812..1054
                     /locus_tag="OO515_003582"
     CDS             812..1054
                     /locus_tag="OO515_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577416.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OO515_003583"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="OO515_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6577417.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OO515_003584"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="OO515_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6577418.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OO515_003585"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="OO515_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6577419.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OO515_003586"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="OO515_003586"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6577420.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIINSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="OO515_003587"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="OO515_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6577421.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8838
                     /locus_tag="OO515_003588"
     CDS             8087..>8838
                     /locus_tag="OO515_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6577422.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2231 c         2265 g         2221 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaatg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgcggc
     8701 gattcaggaa attaatgata agcgccagct tgcggaactg aaaaatattg aagaaagaga
     8761 agggaaaaca ttccactact actctctggc agtcatgatt tcagcgaaac aaattaataa
     8821 cctgatttcg caagagaa
//
LOCUS       DAJTXQ010000154         8810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000154 DAJTXQ010000000
VERSION     DAJTXQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.154"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..766)
                     /locus_tag="OO515_003589"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="OO515_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="OO515_003590"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="OO515_003590"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6577423.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="OO515_003591"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="OO515_003591"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6577424.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO515_003592"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO515_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6577425.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO515_003593"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO515_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6577426.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="OO515_003594"
     CDS             complement(4189..4593)
                     /locus_tag="OO515_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6577427.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="OO515_003595"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="OO515_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6577428.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO515_003596"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO515_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6577429.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO515_003597"
     CDS             complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO515_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6577430.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2333 a         2187 c         2259 g         2031 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga ctttccgtgt
//
LOCUS       DAJTXQ010000155         8633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000155 DAJTXQ010000000
VERSION     DAJTXQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.155"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OO515_003598"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="OO515_003598"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6577431.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OO515_003599"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="OO515_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6577432.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="OO515_003600"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="OO515_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6577433.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OO515_003601"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="OO515_003601"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6577434.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OO515_003602"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="OO515_003602"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6577435.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /locus_tag="OO515_003603"
     CDS             complement(7825..8136)
                     /locus_tag="OO515_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6577436.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OO515_003604"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="OO515_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6577437.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAJTXQ010000156         8607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000156 DAJTXQ010000000
VERSION     DAJTXQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.156"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..3804)
                     /gene="hrpA"
                     /locus_tag="OO515_003605"
     CDS             complement(<1..3804)
                     /gene="hrpA"
                     /locus_tag="OO515_003605"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6577438.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMI"
     gene            4005..4610
                     /gene="azoR"
                     /locus_tag="OO515_003606"
     CDS             4005..4610
                     /gene="azoR"
                     /locus_tag="OO515_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6577439.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4661..5977)
                     /locus_tag="OO515_003607"
     CDS             complement(4661..5977)
                     /locus_tag="OO515_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6577440.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(5967..7724)
                     /locus_tag="OO515_003608"
     CDS             complement(5967..7724)
                     /locus_tag="OO515_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6577441.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(7740..8603)
                     /locus_tag="OO515_003609"
                     /pseudo
     CDS             complement(7740..8603)
                     /locus_tag="OO515_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2177 a         2381 c         2027 g         2022 t
ORIGIN      
        1 tatcatccaa cggatctctt tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt
       61 gatccattgc tgccacgcct gctggacgtt ttcgactttc agcatctgag cacggtcgcg
      121 atgtggatca accgccagtt tttccagccg tttttcaatc gcctgcaaat atcgcagcgt
      181 gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg cgatatacca gcccgcccat
      241 ctgcgcttta atgtcagaaa gccccagcgc catcgtcata tccacccgcc ccttcagacg
      301 tttgttgata ttgaacactg ccgtaaggat ttgctcgacc tgcttcgcaa tatccaccac
      361 cgtgtcgttc agttcggcac gcactttttc atgcagcgca gcaaagcctt cttccgtcca
      421 gaccgggcca ccattggcgt cgatcaattt atccacaccg caggagatac agtcgtcgat
      481 cagctccagc actttgccat acgggttaaa gtacagcccc agcttggctt tgtttggtaa
      541 cttctcatgt aaatacttga tcggcgaggg aatattcagc agcagtagac ggcgaagacc
      601 gttccacatt acctgttttt gctccagcgg attatcaaac agtttgatcg ccacactgtc
      661 gcgttcatcc actagcgctg gccacgcctt cactttgtag ttgccacgct tctgttcgta
      721 gctttccggc agctgaccaa aactccagat atgcagtccg ctctgctcga taccgtcatc
      781 cgccaccgca gacagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag
      841 cgagcgccct tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag
      901 gtgatcgggc acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg
      961 ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa
     1021 aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga
     1081 tttaatcaga gcaatcacca attcgcggcg cagaccgggg atctgccatt caaacccgct
     1141 ttcctcaacc tggttaagta acggcagcgg aatatgtacg gtcacaccgt ccgcgtccgc
     1201 gcctggctca aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg
     1261 gtaatccagc tttctgattt tttccgcccc ttctttgatc aacatgctct tttcaaagtt
     1321 gagcaaatca ggcgtttcgc ggctgacttt tttccaccag ctatcgaagt gacgagcgga
     1381 gattacatcg tggctgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag
     1441 aatatcgcgg cgacgtgatt tgtgttccag ctcttccact tcggcccgca gtttcaggtt
     1501 ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc accagagcgt ggcgaataaa
     1561 gagttcacga cataacgccg aatcgatctg gctgtagttg accttgcgcg cagcaacaat
     1621 cggcaaacca taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca
     1681 gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca acgggttcta cccattccgg
     1741 gtcgatacgc gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat
     1801 tacccatttc ggcggctttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt
     1861 acgtgcaccg gtatattcct gtttatcggc atctttcatg ccgatatggg aaagcaaacc
     1921 ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt tcgctgttga ccggaatgcc
     1981 aagttctttc accacctggc gcaactgggt gtagatatcc tgccattcgc gcacgcgcag
     2041 atagttgagg taatcggtac gacacaggcg acggaaggcg ttggaagaaa gcgccttttg
     2101 ctgctcgcca agataattcc acagatttac aaacgcgagg aagtcggatt ctttgtcgtg
     2161 gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc atcggacgtt cgcgcggatc
     2221 ctgaatggag agcgcggacg tgataatcat cgcctcacgc acgcagccat gtttttgcgc
     2281 ttccagcacc atacgcgcca gacgtgggtc gacaggcaac tgcgagagct ggcgaccgag
     2341 cggcgtcagt ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag
     2401 cagacgcacg ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc
     2461 gatatcgccc agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag
     2521 aatctccgga tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat
     2581 acagatccct tcggacacac gaccacagcg gcctttacgc tgattggcag acgcctgcga
     2641 aatgggttca atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc
     2701 tgtaccgggg tcgatgacgt atttaatccc cggtacggtc agcgaggttt ccgcgacgtt
     2761 ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac acgcggttct gttcgctgtt
     2821 cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc
     2881 atcggcggta tcgcgaattt cccgctcacc actcataaag atcagaatgt cgccagggct
     2941 ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc
     3001 atcggcttct tcaacaatcg gtcgatagcg cacttccacc ggataggtcc gaccggagac
     3061 ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga
     3121 agtgataatg atttttaggt caggacgccg cggcagcaac tctttcaaat agccgagcag
     3181 aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata atgatggtgt cgtactgcat
     3241 cagcaggcgg tcttgctgga tctccgccag caggataccg tcggtcatca gcttgaccat
     3301 cgtgttatca cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc
     3361 cgttttcagc tcttccgcaa tacggttcgc caccgttctt gccgccaggc gacgcggctg
     3421 ggtatggccg atcagccctt taatcccgcg ccccagctcc atacagattt tcggtaactg
     3481 agtcgtttta ccagaacccg tttccccggc gacgatcacc acctggtgat cacgaatcgc
     3541 ttcgagaatg tcctgttttt tctgactaac cggtaaattg tcaggataag taatttccgg
     3601 tcgtgccgct tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc
     3661 ctggaaaatg gcctgttgtg catcaggatt tttaaccttc ttcacgccgt gcagacggcg
     3721 agaaaaacgc agtctgtcac gcagcatcag cgaatccagc cgctgctgca aggccgtaaa
     3781 ggtcaatttt tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc
     3841 tttgaaatga tagatataga gtaccacatc acggctttct atgcgttgtt caataaattc
     3901 gaacataggc ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcaac
     3961 aagcaacggg gcatcgccca ttcaaacatc tataaggaaa caccatgagc aaggtattag
     4021 ttcttaaatc cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg
     4081 ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctggctgcaa
     4141 atccgattcc ggtactggat ggcgaactgg ttggtgcact gcgtccgagc gatgcgccgc
     4201 tgactccgcg tcagcaggaa gctctggcgc tttccgatga gctgattgcc gagctgaaag
     4261 cccacgacgt tatcgttatt gcggcaccga tgtataactt caacatctca actcagttga
     4321 aaaactattt tgacctggtt gcacgcgcag gcgttacttt ccgctatacc gagaacggtc
     4381 cggaaggtct ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca
     4441 aagatggacc aacggacctg gtgacgccgt atctgtccac gttcctcggc tttatcggca
     4501 ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga
     4561 aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt tgctgcataa ccggacacga
     4621 tcttgatgtg gactgtagtc cagtccacat cccaatccac ttacctgttt tcccataatt
     4681 tcagcatcgc tttgtgatct tccttaagca caatatgcga gcgtctggct cgaataaagc
     4741 taatcgcttc atcaacggtt ttacagtatc cgtaacataa cagccatgcc gccactacca
     4801 gcgcactgcg cgataatccc agtgcgcaat ggaccagaac gctgccttgc tcttcgcgta
     4861 atgtttccag catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcta
     4921 gcatcggtac acagaaatag agtcgatctt ttgtcgctcg tccacgaggg aattcaaagg
     4981 tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac
     5041 cagcagtcat tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gatagccaca
     5101 tcccgatgcg ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat
     5161 ctttccctgt tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata
     5221 atactggcca acataaccag actgaccacc ataactgaac catcatcatt agctccatca
     5281 acacaatgca cgcgcacgcg cctacgacat atggcagtgc tattttgatt cgacgttgat
     5341 caggtttctg ataattccaa cgacggtcga cgggcaccat ccagtcaatc aacataccta
     5401 ccgccagccc tgtgatgaca tcaataaaat gatgctgcca tgtcgtcagc gtcgaaatgg
     5461 cgatgagtaa aaaccatccg ccgcagactt tacgccacct cacagccaga tgctgacgaa
     5521 agtgacgcca aagtagccag cagagaataa tatgcagcga aggagactgg ttataaggca
     5581 gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac cccactcact tcaggacgga
     5641 taaaactaaa cttcagcgga tagagcaaaa aaccgcagca ggccattacc gttgccagaa
     5701 taagccggtg gacaagtcgg cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc
     5761 catataaaag atccagactc cagtaaggaa caatagtcca gggaagaaaa gggatcgccg
     5821 tttcccaacc gaatacctga ctgggaatat catggctatt actgtcctga accgcggtga
     5881 actgattaag agatccatag gtgaagaaga aaaacggggc cagcaacaat aaccagccag
     5941 ctccttgtag caatacgttc cggcgttcag ttatcacgac gcaccgccat cgaaaccgta
     6001 aaaatgcccc actcatcaat ccgttgtgtg catttatcaa atccagcatc atgcacgagc
     6061 aaatccatct ccccttgcgt acgcacgcgc attacccaca gtttaccatc tttatgactg
     6121 gttaacaccc cggcaatcag ctccagttgt gggtgccacg gctgcccggt gtagatgaga
     6181 atgccacccg gttcgatggc attggcaaga cctgcgagtg agttttttac ctgctcattt
     6241 tcgggaaaca gctcatacag tccagagaca atcgccagcg taggccgcgg agttaacgcg
     6301 ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac gcacccgccc agacattccc
     6361 cgttgggcaa tcatctcttg cccctgtgca acatttaact cactgtaatc acgtaataaa
     6421 atatcgctta cggcaggctc gtttgccagt gcatccagta catagcgccc atgccctgcg
     6481 gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga gatcggcaac ggcttgttta
     6541 atcagtattt gtaaatgagt tttgcgctgg cgaataccgc gccagccaac actgttcagg
     6601 taaattttgt cgattaagcg cccgaatgcg ttactgcctt gcggttgatt ttgatagaca
     6661 taatccagcg aactgcctga atcaaagcca gtgctcattc cgagatgcag gcccgcagag
     6721 tgcgtcccga acaatttcat cgctttacgc ataaagcgat acgccaaatc aaccggcgat
     6781 aatggcacgg gtccacctga aaggaggcgc cagcgatccg ctgatggtcc agtgcggtct
     6841 tcatgctgat aatcaaattt ttgtgacttg ttagcatata aacgacgaat aaagctttgc
     6901 attttttcaa atgcctgcgc cctgttctct tcacccaacg tgtcgtgata aaagcctggc
     6961 agcagatgca gctctttcag agggctacgt aatctctgat aaaaatcaat ctgcggttgg
     7021 cgatgcacca catagtcatc gcctgatatc agaagttgcg tggggagcgt aatcgccgcc
     7081 gcatctctaa taatacgttc agacgtttta tagagatcga gcaagatatt gacggcaatc
     7141 gcccgtgtga tcagcggatc attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa
     7201 tagcgtcttt tcacatagga attaataaaa aacagaccac gcagacgatg ccatagcgtc
     7261 agcgcaggac gtgccagcgg cacatacaat ttaaccttaa aggccggaga agccagcacc
     7321 agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg caaccagcac tgcgccgacg
     7381 ctttgcgcga tcacaaccac ctcttccagt ccaacctggc tgtcgctggc agcaaaacgg
     7441 acaaattcat cgacatcctg cactgaacgc gcaagagatg ggctataacc acgcggcccc
     7501 gaagtttttc catgcccccg ggcatcccat gcataaaaag cagtatctgg catcgccagt
     7561 tcatcaacga tatgttgtag acgaccagaa tgttcatggc cacgatgaaa taagacgatg
     7621 acctttttcg cccctggctg taaagcgggc cagtgccgat aaaacaacgc tgtattatca
     7681 ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt ccattttctc tattttcctt
     7741 cagtaacagc agtaacgaat aaaataaaaa aataccggag cggtgaaaat taatgagtca
     7801 atcctgtcaa ggattccgcc atgtccaggt aatagtttcc cgctatcttt aacaccaaca
     7861 tctcgtttga tggctgacat cactacatcg ccgcaaaaac cactaatacc aattaacaaa
     7921 cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta acggtccaat gatcatcgac
     7981 gctatcatgg tggtgattac gccgccgacc agaccttcca gcgtttttcc cgggctgact
     8041 ttggggacca cttttcttct gccgcaggat ttcccccata aatactgtgc aatatcgttt
     8101 gacgcggtta atgccaaaag aaataacacc agtaaggccc cgccctggat attcatggat
     8161 ggcaacatta ataactaggc ggcatgactc aaggcgaaaa cggtcattat ccagccccaa
     8221 aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac ccacaaaaac tcgccaggcg
     8281 gctaatatca gaaacccagc caaaggtata aataacagaa acagctcaaa gcaattaaaa
     8341 ccgataagca aatagttaag aggaataacc caatataacc accgcggaaa atgtacggat
     8401 ataagcatac aatattcttt caatgccaga aaacttatca gcgcaaaaaa cgccaacgtc
     8461 atccagcgtg gacctgaaat caccagtgaa aaacaaatga cgatgcccca ccaggtacga
     8521 atacgtaatg taacctcacc cccttttctc tccggtaacc gccatagcag aaaacggttt
     8581 atcagcgttg ctaaaattaa caacgcg
//
LOCUS       DAJTXQ010000157         8580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000157 DAJTXQ010000000
VERSION     DAJTXQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.157"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1161
                     /gene="rtcR"
                     /locus_tag="OO515_003610"
                     /pseudo
     CDS             1..1161
                     /gene="rtcR"
                     /locus_tag="OO515_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1143..1901)
                     /locus_tag="OO515_003611"
     CDS             complement(1143..1901)
                     /locus_tag="OO515_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6577442.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(1918..2748)
                     /gene="glpG"
                     /locus_tag="OO515_003612"
     CDS             complement(1918..2748)
                     /gene="glpG"
                     /locus_tag="OO515_003612"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6577443.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(2793..3119)
                     /gene="glpE"
                     /locus_tag="OO515_003613"
     CDS             complement(2793..3119)
                     /gene="glpE"
                     /locus_tag="OO515_003613"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6577444.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3309..4814
                     /gene="glpD"
                     /locus_tag="OO515_003614"
     CDS             3309..4814
                     /gene="glpD"
                     /locus_tag="OO515_003614"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6577445.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(4866..5471)
                     /locus_tag="OO515_003615"
     CDS             complement(4866..5471)
                     /locus_tag="OO515_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577446.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5474..6241)
                     /locus_tag="OO515_003616"
     CDS             complement(5474..6241)
                     /locus_tag="OO515_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577447.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6244..6954)
                     /locus_tag="OO515_003617"
     CDS             complement(6244..6954)
                     /locus_tag="OO515_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577448.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(6970..8475)
                     /locus_tag="OO515_003618"
     CDS             complement(6970..8475)
                     /locus_tag="OO515_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6577449.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2215 a         2217 c         2120 g         2028 t
ORIGIN      
        1 cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg atttaagccg ttataacgcc
       61 atcgccagcc gctttgccga ggaacgccag caaacgcttg attttcttaa gtccggcatt
      121 gccacgcgta acccccactt caaccgcatg attgagcaga tcgaaaaagt ggcgatcaaa
      181 tcccgcgcgc cgattctgct taacggtcca accggcgcgg gcaagtcatt tctggcgcga
      241 cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg gcgcgtttgt ggaagtgaac
      301 tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt ttggtcatgt aaaaggcgcg
      361 tttaccgggg cgcggtaatc tcgtgaaggt ttattacgca gcgccaacgg cggaatgttg
      421 tttcttgatg agattggcga actgggcgca gacgaacagg caatgctgct aaaagccatt
      481 gaagagaaaa ccttttaccc gtttggcagc gatcgccagg tgagcagtga ttttcagctt
      541 atcgccggaa cggtgcgcga tttgcgccag ctggttgccg aaggcaaatt tcgcgaagat
      601 ctgtacgcgc ggatcaatct ctggaccttc accctgccgg gtctacgcca gcgccaggaa
      661 gatattgaac cgaaccttga ttatgaagtg gagcgccacg ccacactcac tggcgacagc
      721 gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt ttgcgacctc tccccaggca
      781 acatggcgcg gtaactttcg cgaactttct gccagcgtca cgcggatggc cacctttgcc
      841 actagcggac gcatcactct ggacgtggtt gaagatgaga taaaccgtct gcgctataac
      901 tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg gcgcagaggc agaaaacatc
      961 aatctcttcg accgtatgca actggaacac gttatcgcta tctgccgcta ggcaaagtcg
     1021 ctttctgcag ccggacgaca gctttttgat gtttcgcgcc agggcaaagc cagcgtcaat
     1081 gacgcggatc ggctacgcaa atacctggcg cgttttggtc tgacgtggga agccgtgcag
     1141 gatcagcaca gctccagttg aatatgatgg tccgtcagca cctgcattac gcttactggc
     1201 ggcggggcgt cggtgtagac ggcatctacc atgctgatgc tgcccatatt gaccatcgcg
     1261 ttacggccaa atttcgagtg atcgacaacc agcataacgt ggcgcgagtt ctcaataatg
     1321 gcgcgcttgg tgcgaacttc gtggtaatcg aactccagca gcgagccgtc gctatcgatg
     1381 ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg agataaaatc gagcgtcgct
     1441 tcgccaatga tcccgccatc gcggctgcgt aattcgccac cggcgagaat aatgcggaaa
     1501 tcttctttta ccatcaacgt gttagcaaca ttgagattgt tggtgacaat acgcaaattg
     1561 ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg tgccgatatc gataaacagt
     1621 gtcgagccgt tggggatttg ctccgccact ttgcgggcaa tcctctcttt ttcttcggtc
     1681 tgggtggctt tgcgatcgtg ccacggcgtg ttaaccgaac tggaaggcag cgccgcaccg
     1741 ccatggtgac gcaggatcag gttttgctcc gccagctcat tgaggtcgcg gcgaatagtc
     1801 tgcgggctga cggagaaatg ctctaccagc tcttcagtac tgacataacc ctgctgttta
     1861 accagttcga taataccgtt gtgacgttgt gtttgtttca tttataaatc cctggaatta
     1921 ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc gctaaaccca cggctaaccc
     1981 ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc cggcgacaat
     2041 ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa atgccacttt gcggatcgcg
     2101 ttcaccacgt agccagacgt agcccatcag cgcatacacc acgccagaaa gcccgccaaa
     2161 ccacggcccg ctgaattttt gctgcacata gccgcttaac agggcgctaa tgagagtaat
     2221 aacaattagc ttaccgctac cgaggcgttt ttccaccgca ccgccgagat accaccacca
     2281 gagcaggtta aagaggatat gcatcagcga gaagtgcatt aacgcgtggg tgaagtaacg
     2341 ccagaactca aatttcagtg ctggatcgaa tggccaggcc agccataaca tcacttcctg
     2401 atcgccgaga atttgcatgg caataaacac caccacgcag gcgatcatca tcacccaggt
     2461 caccggacct gcgcgttcac gcaaggcggc aaagaaagga taacggcgat aatgcaggcc
     2521 actgccggta tggcctgcct gccagctggc cgccagataa cgcggatctg ccgggttttc
     2581 gagaaaacgc gccagctccg cccgtacgcg ctcggcctgg gactcatccg ccagccagac
     2641 atcgctttgg ttatgttgtt gaatcgtgag gataacaccc tgcgtcgcca tgtaatcaac
     2701 aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc atcaacatcg ttgctgtcgc
     2761 ttattccaca caaaagggga cagtataaag cgttacgcgc cgtacgccac ctctgcggga
     2821 aactgacgtt gccaggcttc aaagccgccg tcaatgctat agaccacatc gtagccctgt
     2881 tgcagcagat actgcgccgc gcctttgctg ctattgccgt gataacacat caccatcacc
     2941 ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg tgtcgttggt taaatggaaa
     3001 gcctgcaccg catgccccat agcgaaactc tgtggatcgc gaatatcgac cagcaccgcc
     3061 tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa ctgatccatg
     3121 cgtctctctt tctttacaaa caagtgggca aatttaccgc acagtttacg tcgaagcggc
     3181 agataaacgc cataatgtta tacatatcac tctaaaatgt tttttcaatg ttacctaaag
     3241 cgcgattctt tgctaatatg ttcgataacg aacatttatg agctttaacg aaagtgaatg
     3301 agggcagcat ggaaaccaaa gatctgattg tgataggtgg cggcatcaat ggtgctggta
     3361 tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat gctggaggcg caggatctcg
     3421 cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc taccttgagc
     3481 actatgaatt ccgcctggtc agcgaggcgc tggctgaacg tgaagtgctg ctgaaaatgg
     3541 ctccgcatat cgccttcccg atgcgttttc gcctgccaca tcgtccgcat ctgcgcccgg
     3601 cgtggatgat tcgcattggt ctgtttatgt acgatcatct gggtaaacgc accagcctgc
     3661 cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt aaaaccggaa attaagcgcg
     3721 gattcgaata ttctgactgt tgggtagacg acgcccgtct ggtactcgcc aacgcccaga
     3781 tggtggtgcg taaaggcggc gaagtgctta ctcggactcg cgccacctct gctcgccgcg
     3841 aaaacggcct gtggattgtg gaagcggaag atatcgatac cggcaaaaaa tatagctggc
     3901 aagcgcgcgg cttggttaac gccaccggcc cgtgggtgaa acagttcttc gacgacagga
     3961 tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg cagccatatt gtggtgccgc
     4021 gcgtgcatac ccaaaagcag gcctacattc tgcaaaacga agataaacgc attgtgttcg
     4081 tgatcccgtg gatggacgag ttttccatca tcggcaccac cgatgtggaa tacaaaggcg
     4141 atccgaaagc ggtgaagatt gaagagagtg aaatcaatta cctgctgaaa gtgtataaca
     4201 cgcactttaa aaagcagtta agccgtgacg atatcgtctg gacctactcc ggtgtgcgtc
     4261 cgctgtgtga tgatgagtcc gactcgccgc aggctattac ccgtgattac acccttgata
     4321 tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt cggcggtaag ttgaccacct
     4381 accgcaaact ggcggaacat gcgctggaaa aactgacgcc gtattatcag ggcattggcc
     4441 cggcatggac gaaagagagc gtgctgccgg gtggtgccat tgaaggcgac cgcgacgatt
     4501 acgcagctcg tctgcgccgt cgctatccat tcctgactga atcgctggcg cgtcattacg
     4561 cacgcactta cggcagcaac agcgagctgc tgctcggcaa tgcgggggcg ataagcgacc
     4621 tcggggagga tttcggtcat gagttctacg aagcggaact gaaatacctg gtggatcacg
     4681 aatgggtccg ccgcaccgac gatgcccttt ggcgtcgcac aaaacaaggc atgtggctga
     4741 atgcggatca acaatctcgt gtgagtcagt ggctggtgga atatacgcag cagaagttgt
     4801 cgctggcgtc gtaaggatga tgcccggttg aagtgcaacc gggctatttg gattccttag
     4861 actattcatt gtaaataatt gaataacggc ccctgagcta aaatcgccaa acttaagcac
     4921 gttacccaat cgccccactt cttcctctgg tttctatttt ttctgcacca atggcaataa
     4981 tatcccaatg caaaaccaag ctgtgcacta atcgggccga caaaatcagt ccaaggctgc
     5041 ggccaaaaag gggaaaaatt caggggaggc cagacaaacg tcagaaagaa atttcggata
     5101 ttttccagca acgctgattt ctctggagaa atataaagaa tgaaattcga gagaattaac
     5161 cagaaataga ccggcgaaaa gaccagccag caatgcagtc tgttttttaa ttcaggaggc
     5221 gttctgcggg agaattgcca accgatgcca gccattacac tgaaaaaaag tgcatattca
     5281 accattaact caagttgtaa ttcctgaaga gaaatacgca caaccaggag cggaaaggag
     5341 aatattattt ctgccagaaa agttaacgtg aatataagca cggctaagac aataaccgtc
     5401 ggccatacca ataatggcac gataacaaaa ccgagaatta cagggggaat ataaagccgg
     5461 aatttaatca tttttatttt cctggtgatt cggatgatgc gtcatacatc gcgcataaac
     5521 actcatcaat tatccagtca tcttcccaga cctgatattc tgtcattggt tcgggctgtt
     5581 ttagcgggtt ctcaatcgct tgccgcgctc tttctttcag taaaggatga ttatttctag
     5641 cccaggcatt gatttgggtg gtaatggcgt tatcgatatc gtcctggtta tctctgatat
     5701 aatcctcaca atatctgttc aataaacagg caagagaaac ggtcgaccaa aagccgggaa
     5761 ctgaattaat ggcatccagc aatggcgcaa gagcgatatc ggcacggaaa aacatttcca
     5821 gcatatactg taattcgaca gcttcatcgc cggataccca atcagtcata tactggcagg
     5881 caaatcgctg taaaattgcc cgttcatccg cacgccagtt ttctttgttg gcaaggttga
     5941 tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg cccctgtgca acaaattcca
     6001 gtaaacgcgg cagaaaatag cggacctcat cgctattttg cggatcaggc cctgggctac
     6061 tattccaggc attgattaag gaatacggga tcgctcgtaa cgatgtactc cgtaatgcct
     6121 tttgttcctg ctgactcagg caatattcac aacaaaccac aaatttctgc ggcacccgat
     6181 aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg catggcgggg ctgggttgca
     6241 tagttacccc ctcgcccgct gatcgcgtgt aaacggttga tagacaaaac gagcattcgc
     6301 cgcgttcagg cgaataaggg cgctacgcga aagggaaaag ctggggcgaa tgcaactatt
     6361 gcagtctcga cagctttcct ctgcgcaatt ttccactgag taatgacgcg tgtagagttt
     6421 cagttcggcc tgcttatcgc gaaatccgcg taaactgtta ttgttatgaa atttccaggc
     6481 atctttaaag acctgaaaga tatagacatc ctcaatccag gagtcacatt ttttaagcgg
     6541 gcgctttgct attatcaccc gctcatccgg atttagcatc gccgtaaatt gtacggtgac
     6601 gtcatttgtc gtgttcaggt cacattgaac atctggtaca tcactgatat aggggtcaaa
     6661 tccaaaatca gcaccacagg cacaaagacg gctaatggtg gcggtatcga gggaaaatcc
     6721 cggccatacc ttgctggagg ttaagccaat atttaatgtg acatcatatc gaagcaatcc
     6781 ctcaagaccg tcagcaccga cgaacttttc cccttcatcc aaggcctggt taataagttg
     6841 ctgaacaatg ccttcatccc agtatgccga tttgcagttg gcaatcgtaa tatccacgcc
     6901 tgtgaaggga tcagtaccgg caaggcttgc aaataatgta ttaactaatt ccatttttat
     6961 tatccagctt taaggtagcg tcttatacat cgggacataa cctacatctt ccaccaaact
     7021 ctgtccctgc ggcgttaaaa accactccaa caatttttgt gtctctgacg tcgtattttc
     7081 ccgcgttacc ataaaggcat cgacgatata gggatatttg ccgttgcgaa tgttttcggc
     7141 agtcggtgca ataccgttaa tcgctagcag ttttatattt ttatcggcgt tcatttgtgt
     7201 tgcgtaataa cggaaggtgt aacctattgc gttgtttgta ttacggtatt cggcaacgac
     7261 tttaatcatt ccctccatca tacttgccac ttctgtttcc tgcggcgaga tcatgcggac
     7321 atttttcatg acctgggatt gcatcaccgt ctggctacca gagtcttccg ggcgttgcca
     7381 ggtctggatc tcctgatcgt taccgccaac agtacgccag ttggtaattg caccactgaa
     7441 gatgtcacgc acctgttgtt cagtcaggga attaaccgga ttatccgcat tgacgatgaa
     7501 aacaaacgct tcacgggcaa aaggcgtgta cagcaaagta acgcccgatt cctcagcgcg
     7561 ttttttctgc ccaccggaag gttgcgccac aaaaataata tcagcatcac ctttaacaat
     7621 cctgttatac gcatcggggg tacgagagct ctccagatat tccctggtgt gaaaatcttc
     7681 cggtataacg cttaaagcat aaaatgcaga ggcataaatt gggtacgccg ccgtagcacc
     7741 atcgagtcgc ggccagtttt gcgtaaactg aatttgcggt ttaccacgca gtggtgtcag
     7801 ttgattattg agtttatcgg gtcgccaggc ccaggtatca atattttcgc gcacaccgac
     7861 accgggattg agctgtttat ataaataacc ctgataacca gcgacaaaac caagcatgag
     7921 aaggataaac aggagggaat tacggcaacg tagcccggat gtaccggtta aaggatgtct
     7981 ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg ccaacgggta taatgactat
     8041 ccaccagtac tcacctccga ctactgagac taacaaaacg cccagccagg ggagcataag
     8101 atcagccaac caacccgatc ccatctggtg aaattgataa tctgccagat gcagcgcgat
     8161 tatccagcca accaggatta ataaaccggg tactaaaaaa agacattgtc gccagacggc
     8221 tatttctctt ttttctggtg cactatcgcc tgcccatact cgccccgaga aaaatagcat
     8281 taatgcgccc agcgccagca taaaaatgtt gagcagaagg taacttgttg gcgtgataag
     8341 gatattacaa acttcaataa taccggcaag tccgacaccc agcatagcaa caaccgccgc
     8401 caaaaattgc gtcagtatgg ggatgccgaa aaaagtcatt accagcgagg acaaaatcca
     8461 ttttctgttt tgcattattc tttccattct ttttgaatgg tgaaattata ctccccgagt
     8521 ccccttgccc cttctggaca cttttccgaa atgatggcgg aaaaaaacgg gaccctttgg
//
LOCUS       DAJTXQ010000158         8573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000158 DAJTXQ010000000
VERSION     DAJTXQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.158"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(270..1043)
                     /locus_tag="OO515_003619"
     CDS             complement(270..1043)
                     /locus_tag="OO515_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6577450.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="OO515_003620"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="OO515_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCR6577451.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="OO515_003621"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="OO515_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR6577452.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="OO515_003622"
     CDS             1926..2666
                     /locus_tag="OO515_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6577453.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="OO515_003623"
     CDS             complement(2637..3404)
                     /locus_tag="OO515_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6577454.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OO515_003624"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OO515_003624"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6577455.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="OO515_003625"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="OO515_003625"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6577456.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OO515_003626"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OO515_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6577457.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="OO515_003627"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="OO515_003627"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6577458.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8573)
                     /locus_tag="OO515_003628"
     CDS             complement(8354..>8573)
                     /locus_tag="OO515_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6577459.1"
                     /translation="NKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVF
                     KAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2065 a         2151 c         2245 g         2112 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttc
//
LOCUS       DAJTXQ010000159         8322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000159 DAJTXQ010000000
VERSION     DAJTXQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.159"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(330..1166)
                     /locus_tag="OO515_003629"
     CDS             complement(330..1166)
                     /locus_tag="OO515_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6577460.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1166..1969)
                     /locus_tag="OO515_003630"
                     /note="aph(3'')-Ib"
     CDS             complement(1166..1969)
                     /locus_tag="OO515_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6577461.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2030..2845)
                     /gene="sul2"
                     /locus_tag="OO515_003631"
     CDS             complement(2030..2845)
                     /gene="sul2"
                     /locus_tag="OO515_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6577462.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4034..4183
                     /locus_tag="OO515_003632"
     CDS             4034..4183
                     /locus_tag="OO515_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577463.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4950..5390)
                     /locus_tag="OO515_003633"
     CDS             complement(4950..5390)
                     /locus_tag="OO515_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6577464.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5608..5997
                     /locus_tag="OO515_003634"
     CDS             5608..5997
                     /locus_tag="OO515_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577465.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6041..6229
                     /locus_tag="OO515_003635"
                     /pseudo
     CDS             6041..6229
                     /locus_tag="OO515_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6196..7395)
                     /gene="tet(A)"
                     /locus_tag="OO515_003636"
     CDS             complement(6196..7395)
                     /gene="tet(A)"
                     /locus_tag="OO515_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6577466.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7501..8151
                     /gene="tetR(A)"
                     /locus_tag="OO515_003637"
     CDS             7501..8151
                     /gene="tetR(A)"
                     /locus_tag="OO515_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6577467.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT         1889 a         2290 c         2298 g         1845 t
ORIGIN      
        1 gaggtccagg gcggccgtct tcacgttctg ccttgcgcag atgagataga tctagcgtgg
       61 actcaaggct ctcgcgaatg gctcgcgttg gaaactttca ttgacacttg aggggcaccg
      121 cagggaaatt ctcgtccttg cgagaaccgg ctatgtcgtg ctgcgcatcg agcctgcgcc
      181 cttggcttgt ctcgcccctc tccgcgtcgc tacggggctt ccaggggtcg tttgcgggaa
      241 ggggcggaat cctacgctaa ggctttggcc agcgatattc tccggtgaga ttgatgtgtt
      301 cccaggggat aggagaagtc gcttgatatc tagtatgacg tctgtcgcac ctgcttgatc
      361 gcggccgcga tagctagatc gcgttgctcc tcttctccat ccgcgttcca agctgcggaa
      421 aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg gatcgacgtc cagcgcacga
      481 gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga gacaaaggtc gtctctgtca
      541 gccggatcgt agaacatatt ggcggcgcca aagcccactt caccgaccag accgacggga
      601 tctatcacca gccagccgcg actggagaac atgatgtttt catgatgcag atcgccatgt
      661 agcccacgca gttccgaggc attgctcatc atttgatcgg ctataatcgc cgcgtggacg
      721 tagtcagttt gacaacctgc gttttgatca tcgcgcgccc gctgaaacaa agctgcaaag
      781 cgatcccgga tcgggagaag ggcagaaggc aggggttcct cagatgcggc atacagcttc
      841 gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc cgtgctcggc aacgatgtga
      901 gagagcattc gctccccggc atattcgagc aacatcagat tgttctcacg accgagcaac
      961 cggactgctc ccctcccatt gcgccatacc agatagtcgg ccccgcgcag ttcatcagca
     1021 atgtcttcta taggtttcaa tcccttgacg attgcaggag tcccgtctgg caatgaaact
     1081 ttccaaacga ggctggaaaa ggtgtccgca atgagaacag gttgcgaaac gtgccaatga
     1141 gcaggaaaaa caggcggcat gaacatcaac cccaagtcag agggtccaat cgcagataga
     1201 aggcaaggcg ttcgcggtcg ggggcttcga tccccaatac attgaatagg acagcgaagg
     1261 cgcgctctgc ttcatctggc gctgcccagt tctcttcggc gttagcaatc atgagtgcca
     1321 aatcggcata gcgatctgct gttccgagcc gcccaaggtc gatcagaccc gtgcattgaa
     1381 gagttttagg gtccaccatg aagttcggca tgcagggatc accatggcaa acaaccatat
     1441 cggtgcgctc ttggtcgagc cgcaccggta gctctcgttc gacacgagcc aaaagatcga
     1501 gctgcggcgt actcttgtcc tcgtccggta agaagtcggg attgacggca ttgcgggaca
     1561 ccacatcaac ggcgcgtccg aacattcgcg acagcctgcg ctcaaacgga cattgatcaa
     1621 ccgataggct gtgaacagcg ccaagttgct gccccattga cggccacgct ttgagcaaat
     1681 ccgctccaga cagatcagcc gccggtactc ccggaattgc cgttatcacc aagcatgcac
     1741 cctcctgttc ctcctgccag ttgatgacct cggggcaagc cacacctcga cctttgagcc
     1801 aaatgaggcg gtcacgctct ccagcgagct caccgcggcg ggaagcaggt gcgattttcg
     1861 cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc agattctccg cctctgacag
     1921 gcaaccagtc agaatgcgat tcaccaaaaa aaatattagt tcgattcaat ggaggttcct
     1981 tcagttttct gatgaagcgc gaatatagag aaatatcccg aatgtgcagt taacgaattc
     2041 ttgcggtttc tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt
     2101 gtgtgcggat gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg
     2161 ccccgacatc ccccggacca cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag
     2221 acagaagcac cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga
     2281 gcgaggtttc gggagcagcc cccagaaaaa accccatgcc gggatcaagg acaaggcggt
     2341 tgcgtttgat accggcaccc gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt
     2401 gatccatgat gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat
     2461 gcataacgac gagtttggca gatgatttcg ccaattgcgg atagaacgca gcgtctggaa
     2521 aaccgcgaat atcattgaga taggccacac cacgcgacaa ggcataggct tgcgtcgcgg
     2581 gttgataact gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc agcaccggcg
     2641 cgatacgcgc gatttctgtg tcggacgaaa caggcgcggc gtcgggattg ctggatgccg
     2701 gaccgaggtc gatcacatct gccccctcgg ccatcagctt acgcgcctgc gcaatggctg
     2761 cgtctggcgc cagataccgg cctccatcgg agaaactgtc cgaggttatg ttgacgatgc
     2821 cgaaaatgat gagcgattta ttcatggggg cttctataat aataataatc gagcatgagt
     2881 ctcatacgga tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt tgcggtgatc
     2941 cgtttcatga tgatgtatct gtagcatttt ggaaataaat atatcatctc tcaataagta
     3001 gatatattta tcttttttgc ttctttggtg ctgcaagtca ccaatacgag ggaaacctgt
     3061 tacggagcgg tttaaccgta atcaacagtg actggtgtca tgttcggaag attaattgat
     3121 agtaatctac aaaggatctt cttgagatcc ttttcaatga atcgtaatct cttgctctgt
     3181 aaacgaaaaa accgcctggg gaggcggttt gttcgaaggt taagtcagtt ggggaactgc
     3241 ttaacccggt aacagggttc acgagaccgc cattaccaaa actgttcttt cagtgtagcc
     3301 gcagtcaggc ctctacttca agacctctta atctatcttc tcgtgcactc ctgttactaa
     3361 tggctgctgc cagtggcgtt ttgtcgtgtc tttccgggtt ggactcaagt tgatagttac
     3421 cggacaaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     3481 gaactgcctt cacggaactg agtgtcaggc gtggaatgag aaaccgcggc cataacagcg
     3541 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ctaccagggg
     3601 gaaacgcctg ggatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     3661 ttttgcgatg cttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc gtggccttat
     3721 ctcttctttg ctaagtgtct tcctggtatc ttccaggaaa tttccgtccc cgtccgtaag
     3781 ccggtaccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     3841 aatatctcct gtatcgcata ttctgctgac gcgccggtgc tgcctttttt ctcctgccac
     3901 atgaagcact tcactgattt tcgcaatcgt ggcaacatag taagccagta tacactccgc
     3961 tagcgctaca tgactggttc agggctgcgc cccgaaaccc gctaacaacc tctcagctac
     4021 tggatatcac cccatgccat ctgaatatga tgaacaactg gattcgactg atacgcagct
     4081 tcccggcgaa tatgaccttt ttgatttaat ggcgcaggtt actgaagaga acctgcatgc
     4141 ccctgtggat tttgggtcgc ctgtcggccg ggaagcgtta tagctttttc gcttgcccgc
     4201 gtacgtggta atactccggt attacctttt atttctttat tttcatgtaa ttacagtgga
     4261 tttttaatac ccgggtatta ctcgtgaaaa cgaaatattc cttttttttc ttttaattca
     4321 tgcagttgcg atgaaaatcg cggctgcgtt aggtgtatga ccattttgag gttgaaaaat
     4381 aatcaaccat ggcgggtgat ttacgcaggt taaggggaga tcgtgacaaa aaaagcgttc
     4441 ctcactgtat cgttcatgcc tgcatgaacg ggttattctt cctgactaca gaattacatc
     4501 gctccttcag ttcatacatc acttttacat cacttataca tcacttttac atcacttata
     4561 catcactttt acatcattcc ttcagctcat acgtcacttt catcctctgt gccatttgta
     4621 catcaccccc ttcctgaacg tgctgacagc gggttttccg gctgcgccgg caacgcttca
     4681 cggccacgca ataaattgcg tccccgttca ccgttttcgg atgggaagaa cgccggaaac
     4741 aggacttacc ccggctgaat ggtgtgaatt tccgcgctat gcacttgtgc gcatactcat
     4801 gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg
     4861 ttttaccggt ttcccgtcag aaacgccctg agggcctctc aggcggtgct gataacagat
     4921 gttatggtaa atcagagtca cctgcactgt tactctgaac cggagcgaaa cagccgcgac
     4981 cagaatcccc gctttttttc ctgtgccagc gcgtcagcca gacggctaat ttcggcctgt
     5041 agttgttccc tctcctcatt tcgcaggcgg ttttctgctt cccgcgccat cttgtcttca
     5101 agcattaggg taacagtctg ctgcagagcg tccagtttct gctgaatgac gtctagctga
     5161 gcattttccg cctggctaac gacagatttg gagtgtccct ccttgtgcct ttttggtgtc
     5221 tcagaagtgc gcagctcgcc ataggcccga atcagttcac tggtctcgaa ctcccggcga
     5281 tcattttttc ctgtgcgata gctgacaagg cctttagcca tgtcacgata taattgcgag
     5341 cgcgactttc ctgttaattt cagtgtctct tgaactgtgt gccatgccat aaaagtgaca
     5401 gtgtctcctg tgtgtaccgt attgtaacag cagaacatat tactgtttta acacaaaagg
     5461 tctgaggctg cccgtgaggc gtgaaaaata cacaaaaaac ctgttgacgg tgtggttttg
     5521 acgggcgtag tatttcgaat actgtatata aacacattgt tataaataca gtttttgctg
     5581 tttttaaaag tcaaagagga taattttatg agtacagagt taaatagcgg tactgtcggt
     5641 ggttttggtc acatgggacc aaatgacccc tacaatgatg gtcatagcgg ctctggaagt
     5701 tcgggaggta gttttaacgg tggtggtaat ggtggccatg ataatagtag tggttcatct
     5761 tatactgcat cctttggtct catcactggc cgacacccga taactgtccc aggtacgaag
     5821 aatattaatt ctgtcgatct ttctgctgtc attaataaca ttatacatca tgaaggtgca
     5881 cgcaataaca tgtacctaga tactgcgaac aatgtaagag tgggagtgac gggcactggc
     5941 tggcaatgtc tagcaacggc aggcatttcg gctgagggta aaagaacttt ccgctaagcg
     6001 atagactgta tgtaaacaca gtattgcaag gacgcggaac atgcctcatg tggcggccag
     6061 gacggccagc cgggatcggg atactggtcg ttaccagagc caccgacccg agcaaaccct
     6121 tctctatcag atcgttgacg agtattaccc ggcattcgct gcgcttatgg cagagcaggg
     6181 aaaggaattg ccgggctatg tgcaacggga atttgaagaa tttctccaaa gcccgcgacg
     6241 cagcgccggc aggcagagca agtagagggc agcgcctgca atccatgccc acccgttcca
     6301 cgttgttata gaagccgcat agatcgccgt gaagaggagg ggtccgacga tcgaggtcag
     6361 gctggtgagc gccgccagtg agccttgcag ctgcccctga cgttcctcat ccacctgcct
     6421 ggacaacatt gcttgcagcg ccggcattcc gatgccaccc gaagcaagca ggaccatgat
     6481 cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg tgccgtcggc
     6541 aatcattccg agcatgagtg cccgcctttc gccgagccgg gcggctacag ggccggtgat
     6601 cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc cgatcgtggt
     6661 cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg gcacctgtcc
     6721 gacaagttgc atgatgaaga agaccgccat cagggcggcg acgacggtca tgccccgggc
     6781 ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt aacggccggc gttcgccttt
     6841 gtgcgactcc ggcaaaagga aacagcccgt caggaaattg aggccgttca aggctgccgc
     6901 ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc ccaccgagca caggtcccgc
     6961 gaccatcccg aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc gctcatcgcc
     7021 atcagtgata tcggcaatat aagcgccggc taccgcccca gtcgccccgg tgatgccggc
     7081 cacgatccgc ccgatataga gaacccaaag gaaaggcgcc gtcgccatga tggcgtagtc
     7141 gacagcagcg ccggccagcg agacgagcaa gaccggccgc cgcccgaaac gatccgacag
     7201 cgcgcccagc acaggtgcgc aggcaaattg catcaacgca tacagcgcca gcagaatgcc
     7261 atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg
     7321 cataatcagg ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg
     7381 tctgttgggt ttcacgtctg gcctccggac cagcctccgc tggtccgatt gaacgcgcgg
     7441 attctttatc actgataagt tggtggacat attatgttta tcagtgataa agtgtcaagc
     7501 atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc
     7561 ggcgtagacg gtctgacgac acgcaaactg gcggaacggt tgggggttca gcagccggcg
     7621 ctttactggc acttcaggaa caagcgggcg ctgctcgacg cactggccga agccatgctg
     7681 gcggagaatc atacgcattc ggtgccgaga gccgacgacg actggcgctc atttctgatc
     7741 gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc gcgcatccat
     7801 gccggcacgc gaccgggcgc accgcagatg gaaacggccg acgcgcagct tcgcttcctc
     7861 tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat cagctacttc
     7921 actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga gcgcggcggc
     7981 accgttgaac aggctccgct ctcgccgctg ttgcgggccg cgatagacgc cttcgacgaa
     8041 gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg attggcgaaa
     8101 aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg gtgacgattg atcaggaccg
     8161 ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc cctccccctt
     8221 tccaccgcgt cagacgcccg tagcagcccg ctacgggctt tttcatgccc tgccctagcg
     8281 tccaagcctc acggccgcgc tcggcctctc tggcggcctt ct
//
LOCUS       DAJTXQ010000160         8272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000160 DAJTXQ010000000
VERSION     DAJTXQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.160"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..936
                     /locus_tag="OO515_003638"
     CDS             52..936
                     /locus_tag="OO515_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6577468.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO515_003639"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO515_003639"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6577469.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO515_003640"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO515_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6577470.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO515_003641"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO515_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6577471.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO515_003642"
     CDS             5160..6443
                     /locus_tag="OO515_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6577472.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO515_003643"
     CDS             6590..8065
                     /locus_tag="OO515_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6577473.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1914 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc gg
//
LOCUS       DAJTXQ010000161         8214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000161 DAJTXQ010000000
VERSION     DAJTXQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.161"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO515_003644"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO515_003644"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR6577474.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO515_003645"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO515_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6577475.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OO515_003646"
     CDS             2028..2546
                     /locus_tag="OO515_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6577476.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO515_003647"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO515_003647"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6577477.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OO515_003648"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OO515_003648"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6577478.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OO515_003649"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OO515_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6577479.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO515_003650"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO515_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6577480.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="OO515_003651"
     CDS             complement(6710..8170)
                     /locus_tag="OO515_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6577481.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2171 a         2055 c         1870 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tatatgtttt gtta
//
LOCUS       DAJTXQ010000162         8182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000162 DAJTXQ010000000
VERSION     DAJTXQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.162"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OO515_003652"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OO515_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6577482.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OO515_003653"
     CDS             complement(2975..3697)
                     /locus_tag="OO515_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6577483.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OO515_003654"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OO515_003654"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6577484.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="OO515_003655"
     CDS             6679..>8182
                     /locus_tag="OO515_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6577485.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2030 c         2321 g         1852 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAJTXQ010000163         8158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000163 DAJTXQ010000000
VERSION     DAJTXQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.163"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..450)
                     /locus_tag="OO515_003656"
     CDS             complement(<1..450)
                     /locus_tag="OO515_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6577486.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
     gene            complement(567..1613)
                     /gene="ansB"
                     /locus_tag="OO515_003657"
     CDS             complement(567..1613)
                     /gene="ansB"
                     /locus_tag="OO515_003657"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6577487.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1789..2508)
                     /locus_tag="OO515_003658"
     CDS             complement(1789..2508)
                     /locus_tag="OO515_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6577488.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2692..3018)
                     /locus_tag="OO515_003659"
     CDS             complement(2692..3018)
                     /locus_tag="OO515_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6577489.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="OO515_003660"
     CDS             complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="OO515_003660"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6577490.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3898..4950
                     /gene="mutY"
                     /locus_tag="OO515_003661"
     CDS             3898..4950
                     /gene="mutY"
                     /locus_tag="OO515_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6577491.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4978..5253
                     /locus_tag="OO515_003662"
     CDS             4978..5253
                     /locus_tag="OO515_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6577492.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5318..6397
                     /gene="mltC"
                     /locus_tag="OO515_003663"
     CDS             5318..6397
                     /gene="mltC"
                     /locus_tag="OO515_003663"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6577493.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6599..7855
                     /gene="nupG"
                     /locus_tag="OO515_003664"
     CDS             6599..7855
                     /gene="nupG"
                     /locus_tag="OO515_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6577494.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7904..>8158)
                     /locus_tag="OO515_003665"
     CDS             complement(7904..>8158)
                     /locus_tag="OO515_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6577495.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2160 c         1987 g         2088 t
ORIGIN      
        1 ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga tatcgtcgac
       61 cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagttcaccg accagccaga
      121 cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt accggtttat ctttcgtcca
      181 ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga gatcgtcact
      241 ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat caagattttt
      301 taaggtgata gcatcaagct tgagaatgcc gtgatactgc tttttgaatt gatcttttaa
      361 gatattttca gttggcggtt cgccatctgc ccaggcatta ccagtcatca acataagcag
      421 tgccgtgcca acaatccatt gctttttcac ccgaaaaacc tcacgcggaa taatttattc
      481 cggtgattat cattagggta atgaaaattg gcaatggacg taagtctgaa gtgaaaaagc
      541 cccggcacga taccggggcg aggtgattag tactgattga agatctgctg gatctgctgc
      601 ggatctttgg tttgcgtcag agccagctgc agcaggacgc gcgctttttg cgggttcagc
      661 gtgccagagg cgacgaagcc gtatttcgca tcatccactt cagcatcctg agtggtagca
      721 cccgtcggta cgcgggaaga acgcactact gcagtgccgt ttttcgcggc ggttgccagg
      781 gtgtcgaaca cggatttata caggttacca ttacccacac cagcgctaac gatgccatca
      841 tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta gttataaacg
      901 atgccgactt tcggcagctc attcagctta gagacatcga atggcgtatc gctggtgtgc
      961 ttacgtgccg gggtacgttg gtagtcaatc ttaccgttgt gaatgtatcc cagaggaccg
     1021 tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac atcgcgaccg
     1081 tccagtacgg tgtcgttcat caccaccagc acgccacgat tagcggaggc tttatcagct
     1141 gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcatgga cgtggacggg
     1201 cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc gaggaagtaa
     1261 gcggtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt tttatcgcag
     1321 tcggtgttaa tttttttcgc cagtgtcagc cagacattat cgttcatgtc ctgggagccg
     1381 atattcacta cctgctcgcc tttaacgttc gcaatgtctt ttagttgcgg caccgcatta
     1441 accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc ggagtcacca
     1501 ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc caatgctgca
     1561 ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc catttcattc
     1621 ctccagttac gtgaacgcta cgcattatcc cttagctctg tatgggaaat ttgacgttaa
     1681 acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg taacaaaacg
     1741 aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt attttaaatt
     1801 cccgaccagg gctttgcggc tatcttccag agtcacaacg cggctacaaa catctttgcc
     1861 aaactgctgg aaatcttttt cctgcttttt ccactctgtt tggattgagg attgcagccc
     1921 ccccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca ccgctttcgc
     1981 gcccatttca ttaatgctgt cctgtaaaat tccacccatt gcctgattca ctaattgctg
     2041 gccttcggcg cgaacctgat caatggcttt atagtgaaac gtcaggccat cgctgcgcgt
     2101 ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca gacggctgcg
     2161 cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa tacgggcttt
     2221 ttcgacgcgg cttttcgcgc cttcatcaat ccacggcagc gtgctgcgta gttcagcctg
     2281 ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac cgttatacat
     2341 cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct gcacggtttg
     2401 cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg cgtgagcggt
     2461 cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag tcttccctca
     2521 agaaaaaatc aggccagcat ttgctggccc cagattgata acaaagtacg cgttttccat
     2581 gccggatgcg gcgtaaacac cttatcccgc ctactcgatc gtgcatcttt ccaggcctga
     2641 taagcgtagc gcatcaggca gttttgcatt tgtcatcgcc cgtatgcttt cttagtccca
     2701 ccaaacgtcg aaaagttcgc tggtgcgtac ctcatccagt ttgcgctctt ccagccactt
     2761 acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc agatcagacc
     2821 ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga taacctcgtt
     2881 aataaaatca tcaacggttt tatcaatctg ttcttccgat gtaccttccg ggaatcgcca
     2941 tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac gcagacgacg
     3001 gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac tccgtgacca
     3061 agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg cggcacgtaa
     3121 tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc aagcatatgt
     3181 tccgcataag gttcccagtc ggtcgccata tggaatacac cccccagctg cagtttgctt
     3241 tttaccagtt cggcaaacgg cacctgtacg atacggcgtt tattatggcg cgctttgtgc
     3301 cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat cattttatgc
     3361 agcacttcaa ccgcatcgtg acacatcacg cgcaggttgc ttaaaccttc ttcatgcgca
     3421 gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa gtcctgctca
     3481 gggcgatctt tagccattgc caccagcgac gcccccatgc caaaaccaat ctcaagcgtc
     3541 accggcgctt cacggccaaa aagcgcgggg aaatccagca tatcttcgct gaactcaacg
     3601 cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag tcgcccctgg
     3661 cggcgcacaa aactacggat acggcgcagt gggcggccgt tttcatcaaa ttccggtgaa
     3721 atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa acgggcatta
     3781 tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa gttccggttt
     3841 acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc ggtgaccatg
     3901 caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg gcgaaaaacg
     3961 ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt gatgttgcaa
     4021 caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg cttcccgacg
     4081 gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac cgggcttggc
     4141 tattacgccc gcgcgcgcaa tctgcataaa gcggcacaac aagtggcgac cttacacggc
     4201 ggtaaattcc cggaaacctt tgaagaagtc gcggcgttac cgggcgtcgg gcgttccacc
     4261 gcaggcgcga ttctctcgct ttctctgggt aagcactttc cgattctcga cggtaacgtc
     4321 aaacgcgtgc tggcgcgctg ctatgctgta agcggctggc ctgggaaaaa agaggtcgag
     4381 aataaattat ggagtttgag cgagcaggtg acgcccgcgg tcggcgtgga acggtttaat
     4441 caggcgatga tggatttggg cgcgatgatt tgtacgcgct cgaagccgaa atgttcgctc
     4501 tgtccgctac aaaacggatg tattgccgcc gccaataata gctggtcgct ttatccgggc
     4561 aaaaaaacga aacagacgct gccggagcgc accggctact ttttgctgtt acagcacgaa
     4621 gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcggttt atactgtttc
     4681 ccgcagtttg ccgacgaaga aagtttgcgg cagtggctgg cgcaacggca gattgctgcc
     4741 gataacctga cgcaactgac cgcgtttcgg cataccttca gccatttcca cttagatatt
     4801 gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg caatgcgctc
     4861 tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga gcgtttgtta
     4921 cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg atgatttatg
     4981 agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga ttttcagctg
     5041 taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc ctgggcgcag
     5101 tggcagcaca agcaaaccat gctgattaat gaaaagaaac tcaacatgat gaatgccgag
     5161 caccgcaagc tgcttgagca ggagatggtc aacttcctgt tcgagggtaa agaggtgcat
     5221 atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac gcctccggca
     5281 acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc
     5341 tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga
     5401 agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg cccacaatat
     5461 tgagaacatc tggggattca aagaggtggt gatcgccggt cctaaggact acgtgaaata
     5521 caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga
     5581 aactatcgcc gggacagaac ctgccgcgca tttgcgccgg gcaattatca aaacgttgct
     5641 gatgggtgac gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc
     5701 gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga
     5761 aggtcgcgca agcaacttcg cggattatct gctgaaaaac cgtctgcaaa gccgcagtaa
     5821 cgggctgcgt atcatctaca gcgtcaccat taacatggta ccgaaccacc ttgataaacg
     5881 tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggcg ttgatgagtc
     5941 gctgattctg gcaattatgc agaccgaatc ttcctttaac ccgtatgcgg tcagccgttc
     6001 cgatgcgctg ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc
     6061 gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga
     6121 taccggcacc gcgtatctgg cgatgctgaa caatgtttat ctcgacggaa ttgataaccc
     6181 aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg
     6241 agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga cgccgggcga
     6301 tgtttatcag acgctgacga cccgccatcc ctctgcggaa tctcgccgtt atctttataa
     6361 agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cattaaccgc ccctgacgat
     6421 gctcaggggc aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc
     6481 aattatttgc cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc ccatcatctc
     6541 gcacggtcaa atgtgctttt tcaaacactc atccgcatca caatgtgagg aaattaacat
     6601 gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg
     6661 gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg
     6721 cgcagtttat agctcactgg gtatcgccgc ggtatttatg cctgcgctgc tggggattgt
     6781 ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat
     6841 cacgctgttc atggcggcac aggtcacgac gccggaagcg atgttccttg tgatattgat
     6901 taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct
     6961 gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat
     7021 cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca
     7081 gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca
     7141 tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc
     7201 attcgcgctg tttaaaaaca agcgtatggc aatcttcttc atcttctcaa tgctgctggg
     7261 cgcggaactg cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga
     7321 tccgatgttt gccagcagct tcatcgtgca gcatgcgtca atcatcatgt cgatttcgca
     7381 gatctctgaa accctgttca ttctgaccat cccgttcttc ttaagccgct acggtattaa
     7441 gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg
     7501 cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt acggttgcgc
     7561 attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat
     7621 tcgcgccagt gcacaaggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg
     7681 cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta ccgactggca
     7741 gaccgtatgg ttgattttcg ctggttattc cgtggttctg gccttcgcgt tcatggcgat
     7801 gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg
     7861 caaagaaaaa cgggtcgcca gaaggtgacc cgttttttta ttcttacttc aacacataac
     7921 cgtacaatcg tttcatgcca tccgcatcgg tttcgctata gacaccttgc aactccggcg
     7981 aaaatcccgg caacaaattc acgccttctt ccagcgcaag gaaataacgt tgaaccgccc
     8041 caccccagac ttccccggga accacgcaaa gcacgccagg tggataaggc aacgcccctt
     8101 ctgccgcaat tcgcccttcg gcatcacgaa tccgcaccaa ctccacttca ccgcgaat
//
LOCUS       DAJTXQ010000164         8099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000164 DAJTXQ010000000
VERSION     DAJTXQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.164"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..796)
                     /locus_tag="OO515_003666"
     CDS             complement(101..796)
                     /locus_tag="OO515_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6577496.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OO515_003667"
     CDS             complement(789..2216)
                     /locus_tag="OO515_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6577497.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OO515_003668"
     CDS             complement(2227..2946)
                     /locus_tag="OO515_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6577498.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEALPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO515_003669"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO515_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR6577499.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OO515_003670"
     CDS             4554..5879
                     /locus_tag="OO515_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR6577500.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OO515_003671"
     CDS             5988..6224
                     /locus_tag="OO515_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6577501.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OO515_003672"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OO515_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR6577502.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OO515_003673"
     CDS             complement(6989..7858)
                     /locus_tag="OO515_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6577503.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2191 a         1982 c         1731 g         2195 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa gcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatgga
//
LOCUS       DAJTXQ010000165         7963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000165 DAJTXQ010000000
VERSION     DAJTXQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.165"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            668..1621
                     /locus_tag="OO515_003674"
     CDS             668..1621
                     /locus_tag="OO515_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6577504.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1621..1917
                     /locus_tag="OO515_003675"
     CDS             1621..1917
                     /locus_tag="OO515_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577505.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2241..2531
                     /locus_tag="OO515_003676"
     CDS             2241..2531
                     /locus_tag="OO515_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577506.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2752..2967
                     /locus_tag="OO515_003677"
     CDS             2752..2967
                     /locus_tag="OO515_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577507.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3114..3419
                     /locus_tag="OO515_003678"
     CDS             3114..3419
                     /locus_tag="OO515_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577508.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3895..4605
                     /locus_tag="OO515_003679"
     CDS             3895..4605
                     /locus_tag="OO515_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6577509.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4630..4836
                     /locus_tag="OO515_003680"
     CDS             4630..4836
                     /locus_tag="OO515_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577510.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4935..7115
                     /locus_tag="OO515_003681"
     CDS             4935..7115
                     /locus_tag="OO515_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6577511.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7272..7860
                     /locus_tag="OO515_003682"
                     /pseudo
     CDS             7272..7860
                     /locus_tag="OO515_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2626 a         1421 c         1703 g         2213 t
ORIGIN      
        1 cataacgggc ttactattcg ttcaccgttt cctagtgcag tcgttgtttt taaaaaagaa
       61 ccacgtaagc acgaaattac acagacggtt tgctgtggtg acacgctatt accgttaacc
      121 atcattaaca gaggaggctt gagaggaaga cccaccattt acccacctga gacgctcgac
      181 agcttcataa aactatacag gcaaggaaaa cccattaagt gcattgcaga tgctctcaga
      241 atgcctctga gcacgtcata caggattgct caaaggttga gctaaaattc tcataaatat
      301 ggtcatttac aagataggtg attcagatat taagaagtat ccctgtgaac aataaatgac
      361 caatcagtaa ttattctgtg aaataattgt tgcaatttat aatcacatct gttatagttt
      421 ttatatgggg tgataaaaga actcagaaca ttaagaggtt acaacgttgg gaggcgtcag
      481 ggtattgaca gattttcacg gcctcttatt caatcaaggc ttttcctttt gctgttaata
      541 ctcgcgtaat cgctcaggct tctgagttcc tttatcagtc catcttctaa ttaattcact
      601 ccctctgggg ttggtcacta tacgtggtca gccctttctt tgttttattt ttaaaaagga
      661 aaatataatg gaaaaattcc acatgtttga tgaccgtatt tatcagatga ctaatggcgt
      721 taaagctgcc gaccgtatta tttacactgt catgttgaat gaacaaaagt tttatcacga
      781 taaggggcaa tattataccc cttcatatgc tcatcttggt cagtatgctg gaatcacaag
      841 aacatccgtg attgcatgta tgaaacaact tgaggcacat ggtttaatca agaaaactaa
      901 cgttgcaagc aatcagtgtg ttattaaagt ttattcactt gatgaagtac cggaggtttt
      961 aacagagctt acagctaagg aagccagaaa ggcaaagctg gagtctaaag ttactcagtc
     1021 agtcaagaaa gatgacccac tgagtcaaaa ttctttaccc actgagtcaa aattttttac
     1081 tcagtcagtc aaaatctttg acacgtataa taacagtaat aacaagaaag aacttattaa
     1141 taacaataaa gaagatgact ttgaagaaga atattttaag aagggagctt cgctctgtga
     1201 ttcacatgaa tgtgattctc ttggtattga agaggctatt aaagagactg ttaaaacatc
     1261 tgacttagat gaccttgata gtgaagctgc taaagaggtc gttaaaaagg aagataaaat
     1321 ctctgatgtg attgctagta gtgaccataa aaacacacag tgccccgcca ctgatgatga
     1381 attaccggat gttgaccttg atgatgaaga agaccctgaa aaggaattga aacgtgaaca
     1441 gcgtaaagca gaaattaaag aaagattcag aaatgcgaag ctaaagaaca aggttagtga
     1501 cctcgatgac tttgatgata gtgacgatga ttttactttt acaccaaaaa agaaaactca
     1561 caccgagcca aagaaagaaa aagttaaagt cagacgtgtt tatggtggag gaatgtttta
     1621 atgaaaactt ataatggcga gagaaaatac cgtgactatt tactcagacg ttacagcatt
     1681 tctcgtgaag gttacttgat gaaagatact cacggggaag tttaccggat tcgaccgaag
     1741 aaagaaggga gggattatta tttcgtcgat gatgtgacag atttaaagat tgacgctttg
     1801 aagtttgcgg tgctgtatca ttacgattta tgggattcag tccaccagct acgattaaaa
     1861 gacggtgacc ccggtaatct gagggccacc aatattatca agggaaagtg ccggtaaggc
     1921 caccagctat ttatttactg aaatctctat ctcaacacga agaagtttaa gtcgaattgt
     1981 aagctttgtc ttttttgtaa tgactcgatt cataacgcaa aacaccaata gcacaataaa
     2041 taccgcaaag cccaacgagc taaaacgatg agattcaata attgctgtga aaaaatcatg
     2101 cattaatttt ccttttgtga aaggaaacag cacctgtatc agcccctaca accatagtat
     2161 catgtaaatg aggtactgac aatatgttaa atagatgtta agattgtagt atgagtaaga
     2221 gatttaagga gatttttaca atgactcaca cagttacgcg ccttttgcca gaggcatttg
     2281 aacgactggc agcaatcaaa gccaaatatc ccgatatgaa gcacacagcg cttgttgggg
     2341 agattttaaa agagtcggta aaccttagcc ctttcgataa cgttaagggc gtcgcagagg
     2401 gttctaaggc catcagaatc acaacgggcc aactggaact actcaaagca cagtccgcag
     2461 tagcaggttg ctcaatgatt caggtgttaa gcgctaaggt ccatcaactg gcggatagat
     2521 tggaggttta atgtgttata ggttccaata ttccacactt ggccgccacc taatcagaca
     2581 gaatttagca gatgctgaat ttgaagatgt gaccctcaaa gcaaacaaac gattaaaacc
     2641 ctctcaaatc caccagctta aacacgaatt aaagccactg gtaatactcg actataacga
     2701 ccctgaacag cttgagcagg gaattaagct tatttctgaa tggggtgctt gatgaaaact
     2761 atcacagtac aacttcagac taataaagca tttcgttatt ttgaaaactt gcttgagtta
     2821 tacgaaggat ggggaagtat ccacggcaaa gatgatatct atcttcactt gtcagcccct
     2881 aactattctc tgaagactcc ggttaagcaa agctggttaa aagactacgg tcaccagatg
     2941 gggctattag tctcagactt gagctaaaaa cagcaaaaca tatctctcag gctttaccag
     3001 acgcaccaga aacgttttta agcccctaat cagggtaact tagggaatcg ccttaagtgg
     3061 tctaaaaaag gggttaaaac gtcttagaat gcgatacagg aaggatttta aaaatgaatt
     3121 acccaatgca aattatggtt gaccaagaaa ccatgaattg gttgattgac ctgaacaggt
     3181 cgggctttaa tatctatttc atgcattgtt ttgaagcgtc aacgtatatt gacaagcata
     3241 taatcaacga aattaaactt ggtgactatg ttcacaagct ggaagtaaca gacccataca
     3301 ataagcgctt tatcattgaa gagtgcaatc gacttgattt aagccctgag caattattga
     3361 agctgaatgt attcttgacc tatcagaatg aattaaaccc ttacagcgag gtgatgtaat
     3421 gacactcgtt attctctgga tgactttagg gttatttgtt ggttgctggg taggtaatac
     3481 ggtaaataag aattgatgtt accaatctgt taaaaagctg ttgacaaata accttaagca
     3541 tgagatgatg gtaataggga agagcaactc ttcctgaatt aaactattaa tcattctaca
     3601 tcaatgatag ttattctgaa aggtgcaatg cttttcgcta catgaccttt caggttagtc
     3661 tgagaggtca tctcgaaaag tactcttgtt cattcgtttg aatccttttt gaatccttta
     3721 gatgttttaa tttcttcacc ttatgaatgg ccctttataa gaaattatct agggctattc
     3781 agcttttaaa aagcatactt ggcaaaccgc cttttaagtc tcttctaagt ttagataggc
     3841 tgtcaggtgt gctttttaaa aactgaaaca cttccgctta gaagaggaaa agcaatgaca
     3901 aatgatattt acattaatgg caaagtgatt gatagcttca gtgactggtc gccacagact
     3961 gaacatccag ctatcactat tcagcgtaaa gagcatgaca agcgtattga gcttgagcaa
     4021 tcgctaaaga tgtcaccaaa aacaaccatc tttgtgtctc ctgagcctca agagatgcct
     4081 gatatttgca agagtgaagc attacttgag cttgaaaaga aatattacca actgctgaaa
     4141 gaacagcgcc ttagattaga tgacgcttac agcaaagtta ccttgatgca atcagctatt
     4201 gagccgtcag aatttgagat tcaggatgaa ttgtcacaga aaccatttgt ctattaccaa
     4261 tacgaagata atgatggttt tggtactttt caagaaaacg tcgcagccgt gattagtagt
     4321 cttcctgctg gacatcgaat tgtgaagatg gttaaggcat caagaggtgc tggtcagttt
     4381 atttacatga ctaataagag cgatgaagaa ctcgctgaaa ttgctagaca gaacatcttg
     4441 gcatcacgta acaagcaact tgataatgtt aagttgtatc tctcaagaga gcttcaggct
     4501 atgaaagagc ttgtgaaaga atatgaagat gctaagaaga ttgctatgca ggctgatatt
     4561 gagcagctta cgaagatttc tagcaaatat gcaaaggcta tttaattaat aattaaaagg
     4621 tggtggtgaa tgattgattt aacagaacgc tatgagctta ttaaagcaat tagtgaacga
     4681 ttgaaagaac aacccaacaa accaaaacga atcaaatcat tcacctcctc taagcaaaag
     4741 aatagaatca ggaaaatagc taagtggtgt cttgataaat taccaagtga ttgtcagatt
     4801 gttggtgaag atggtaatta ttatctggtt aggtaacaag attggttggg tgaaaatcta
     4861 cgggagagat tatcgagtga tatcggtagt ctttcccttt ttcgtatatg aacacttaaa
     4921 gaggtgaaac atgaatgagc aaattcacag ttgatagttt tatcgttgaa cttagtttca
     4981 gtgaaaacgt tattaagggc ttgcaaagag ttgaaaaggc agctttgcag tcagcacaaa
     5041 gaattgaacg caatttgaat agagcgttta aggtcgatac caagcaactt gacagcaatc
     5101 tgacaagttc tttagggcag cttgaaagga agtttaataa gacctttgac aagattgagc
     5161 agagagcaag aagcacaaaa gcatttcagt ttgccaccag atttaatgac actgttaatc
     5221 ctccaaaaca atcaaggcaa ccacgaatta gcggaaacag agcaatcaca gcagcgcata
     5281 gcgtgaatat gtctaagctt ggtgatatta atccgctgat ggctaaaatg tttaaagcac
     5341 agtattacag cttaagcggt aaggctggta atattggaag tgaaaagttt aacgctgaac
     5401 ttgcaaaatt aaatcaatct cttcgtgaaa cgttgaataa actcaggtca cagacaaaga
     5461 ccgccagcat taacaataca agcgacgcat ttaataatct tgcaagcaat gcgattaaat
     5521 tatctggtgc attttactct gtgatgggtg ctcttaacgc ttacaaggcc attatgaatg
     5581 caggtcttaa acgagattcc gcacagagag ccgctaagtt tgtacttggt gataaagcat
     5641 ctgaagctga ggtatttatt cgaaatttag cagacaagac aggtcttaat atctctgaag
     5701 ggctatcaag ctacgctaag tttgcagcag gtgctcaggg ttctatgtca caggaacaga
     5761 cacaggagct atttggtaat gcaacggcta tgagtcgctt aatggggctt tctaacgatg
     5821 agctaaacgg cattttgaga gcatttgagc agatggcgag caagggcaag attcaggctg
     5881 aggaattacg tgggcagtta ggtgaccgta tggctggtgc atttaagcta ttcgctgaag
     5941 cattgggtat gactgctaca gagcttgatg cagccatgaa aaacgggaag gtactttccg
     6001 ctgatactct ccctaaagtc gctaaacaga tgggcttgat gattgataaa gcgggtggct
     6061 gggctgaagt cactaagagc actcagacag ctttaggtaa gttagcaaac aattgggatg
     6121 atacgatggt gaaaatcttc tcaggttcac aggatgagct aaacggcttc ttatccagcc
     6181 tgagtaacct gcttagtgaa atggggatga gttcttctat cgctggtgat gctatcggtg
     6241 gacttatcga catgcttaaa gctggtgtag atgatattcg cgtattcaat aacaccattg
     6301 aaggctggat tttacagact aagaagttct attacagcct tgatgacact aaacgaaaac
     6361 tacttgatga agtcggtgat ggttttatca acttcgtcaa aggtcttgct atagcgttat
     6421 ctgcaaagac gctattcagt gctactacag gggttatgaa cctgacaaga gcaatcacaa
     6481 cgttaggtac aagagctaat caggtggctg gtcaggtagc tactggtaaa ggtggtggaa
     6541 aactgaaagg tgtagctggt ggtgtaggta gtgctcttgc aattggttat gctgatgacg
     6601 gttatgaaac agcgttagca ttagcttcaa tgattccaca aatcagaggc gtcactcttg
     6661 gtttatatgc attgaagaaa gcactggatt ttatgaatca ggaagtagtc aaaaacgcta
     6721 atatgcatcc gtctggggta ggtgtgggta gtgactttaa tcccgtgtat tctggtgacc
     6781 ctaataaacc gaatatgatt aacgaaggat ggagtagaat ttttagttca atgggtgaat
     6841 tgtttaacaa tgccacaatg aatattgcaa gattcaatca tccagagtta aacaacatta
     6901 agcaagattc agcattaacg agtgcagata ttcagggtct gagggatgaa attagtgctt
     6961 tgagtaagcg tattcaggag ccagtgaaag tttcacttgg tggtcaagtt gtcgttcgac
     7021 cagatgaacc atctttcttt actttcaatc aatcagctaa tgaccagttc acgcaggata
     7081 tgctcttaag ttcaagctat ccagaggaag aatgatttaa cggtgttcta cgtgaataaa
     7141 gcaaagttaa tacccacacc aaaaattatt tttcacgtag ctcacgctta acagagaaaa
     7201 tacatatata tcaaaggctt ggttaaaaat gattaaaata cactaagcct ttgaaatcaa
     7261 aggagaattt aatgatttac actaatcagc ttcgttacag tggcaaagat ggcatctatt
     7321 tccacttaag agacaatgta gatgccttct taaccttatc agcaactgaa aacatggaat
     7381 ttgatagccc tatgcaggtg actacacaga acatgcaatt atgaaggaag cttattatta
     7441 actcgtcagg gtcaattagt agaaaacttc attgacactc tggaaaactg gcgtgaccag
     7501 aagcagatta tttctgttat ctgtaaagat ggaattaaaa ttgacgattc cattattacc
     7561 aactttaaag cctctaaaga tgttactatc tctaacggtc ttcgtatcca gctaactttt
     7621 caggaaatta actttaaagc aatcgtaggt caaactgata tttcagcagc cactggcaaa
     7681 acggctacca caaatgacgg tggtgtgacc agtaagaaga atgcaggtaa cgtgaccact
     7741 gaacgcaata acgcgcctat gctatcgtgc aaagagttaa tgaatattag cgatgtatcc
     7801 ggttacgctc cagctatcag aaatgatatt ctcgatgctc agattaaatg taataaggct
     7861 tggaagctgg actattcaga cttaacagaa gctgaaaaaa ccaacgtcac caatgctaat
     7921 agtaacgctg gtcgagtagc ttcaaagctt aaaaagtact cag
//
LOCUS       DAJTXQ010000166         7960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000166 DAJTXQ010000000
VERSION     DAJTXQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.166"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            267..1925
                     /locus_tag="OO515_003683"
     CDS             267..1925
                     /locus_tag="OO515_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6577512.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFARSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OO515_003684"
     CDS             complement(1922..2878)
                     /locus_tag="OO515_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6577513.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OO515_003685"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OO515_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6577514.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OO515_003686"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OO515_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6577515.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO515_003687"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO515_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6577516.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..>7960
                     /locus_tag="OO515_003688"
     CDS             6789..>7960
                     /locus_tag="OO515_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6577517.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERL"
BASE COUNT         1946 a         1888 c         2174 g         1952 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccggtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga
//
LOCUS       DAJTXQ010000167         7945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000167 DAJTXQ010000000
VERSION     DAJTXQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.167"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..359
                     /locus_tag="OO515_003689"
     CDS             <1..359
                     /locus_tag="OO515_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR6577518.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="OO515_003690"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="OO515_003690"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6577519.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="OO515_003691"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="OO515_003691"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6577520.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="OO515_003692"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="OO515_003692"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6577521.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="OO515_003693"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="OO515_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6577522.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /locus_tag="OO515_003694"
     CDS             complement(5490..6926)
                     /locus_tag="OO515_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6577523.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="OO515_003695"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="OO515_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6577524.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2030 a         2086 c         1888 g         1941 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgac
//
LOCUS       DAJTXQ010000168         7936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000168 DAJTXQ010000000
VERSION     DAJTXQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.168"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OO515_003696"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="OO515_003696"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6577525.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="OO515_003697"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="OO515_003697"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6577526.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="OO515_003698"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="OO515_003698"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6577527.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="OO515_003699"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="OO515_003699"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6577528.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="OO515_003700"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="OO515_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6577529.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="OO515_003701"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="OO515_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6577530.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="OO515_003702"
     CDS             5724..5885
                     /locus_tag="OO515_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6577531.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="OO515_003703"
     CDS             6007..7080
                     /locus_tag="OO515_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6577532.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="OO515_003704"
     CDS             7077..7859
                     /locus_tag="OO515_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6577533.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1895 a         2047 c         2152 g         1842 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc atatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gttttgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc
     2581 ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggatgc cggtaagact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggctcct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 agcgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt gatcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc ggtctggatc
     7381 tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtatacac gacaaactgg
     7501 cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagc
     7861 ctacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctac
//
LOCUS       DAJTXQ010000169         7777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000169 DAJTXQ010000000
VERSION     DAJTXQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.169"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1780)
                     /locus_tag="OO515_003705"
     CDS             complement(<1..1780)
                     /locus_tag="OO515_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6577534.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO515_003706"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO515_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6577535.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO515_003707"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO515_003707"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6577536.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO515_003708"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO515_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6577537.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO515_003709"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO515_003709"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6577538.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="OO515_003710"
     CDS             complement(5968..7005)
                     /locus_tag="OO515_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6577539.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="OO515_003711"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="OO515_003711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6577540.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1872 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAJTXQ010000170         7733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000170 DAJTXQ010000000
VERSION     DAJTXQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.170"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1040
                     /gene="yjeM"
                     /locus_tag="OO515_003712"
     CDS             <1..1040
                     /gene="yjeM"
                     /locus_tag="OO515_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6577541.1"
                     /translation="IARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASP
                     NPGYQSGLAMLSFVVFAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSL
                     AIFLWGVSTNWQQVLSNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARI
                     TGLSMFLAYTGAFFTLCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVT
                     VFILLVSFGGGTASAFFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTR
                     MSAMIAMVVVVLVVTFANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCS
                     RMANKPELALD"
     gene            complement(1191..4514)
                     /gene="mscM"
                     /locus_tag="OO515_003713"
     CDS             complement(1191..4514)
                     /gene="mscM"
                     /locus_tag="OO515_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6577542.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4536..5504)
                     /gene="psd"
                     /locus_tag="OO515_003714"
     CDS             complement(4536..5504)
                     /gene="psd"
                     /locus_tag="OO515_003714"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6577543.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5601..6653)
                     /gene="rsgA"
                     /locus_tag="OO515_003715"
     CDS             complement(5601..6653)
                     /gene="rsgA"
                     /locus_tag="OO515_003715"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6577544.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6748..7293
                     /gene="orn"
                     /locus_tag="OO515_003716"
     CDS             6748..7293
                     /gene="orn"
                     /locus_tag="OO515_003716"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6577545.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7504..7579
                     /locus_tag="OO515_003717"
     tRNA            7504..7579
                     /locus_tag="OO515_003717"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7537..7539,aa:Gly,seq:gcc)
BASE COUNT         1784 a         2020 c         2079 g         1850 t
ORIGIN      
        1 aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat ttagtattgc
       61 tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag gatattaatt
      121 tccttgcatc accgaacccg ggttatcagt ccggtctggc aatgctatcg tttgtggtat
      181 ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat aaaacggaaa
      241 atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt tcaatcggtt
      301 attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta ttaagtaatg
      361 gttccgttaa ccttggcaat attacctatg tgctgatgaa gagcctcggg gtgacgctgg
      421 gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg tttgcgcgta
      481 ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt ctttacgctt tgctattcac
      541 cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg atgacgcgcc
      601 tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgcgggttg gttactgtct
      661 tcatcctgct ggtttcgttt ggtggcggta ccgcatccgc gttctttaac aagctgacgc
      721 tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct ttcccattct
      781 ttaaagcacg tcaggatctc gacagaccat ttgtgatttt caaaacgcgt atgtcggcaa
      841 tgattgcgat ggtggttgtc gtactggtgg tgacatttgc gaacgtcttc accattattc
      901 agcctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc ggccctgtct
      961 tcttctcgct gttagcgatg gcgatttacc agaactattg cagtcgcatg gcgaataaac
     1021 ctgagttagc tctcgactga tcatatgaaa tcctgcaact catcgtctct ccggcgatga
     1081 gttgtcagga agtaatgcgt atggatgata ctctccaggg ccggtgataa ctgaagatga
     1141 tgttaagctg ctaaatgagc cacgttgaca tcatttggca gcttcatgca ttacaaactt
     1201 cccgcctgac gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg
     1261 ctttccagac gcatctggaa gggcgggaat ggcatatcga taccgtgggc atggaagcca
     1321 gccagaatca gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggca
     1381 taaatacgca gctcgaaaat ctgaatccct tgttgcagat ccaccaggaa gacttccggt
     1441 gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact
     1501 tcttcactgt tggcatcagc aggggccgga atcgtcaaca ctacgcgcgt gaccgagtca
     1561 gagagcgacc agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct
     1621 ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggtg
     1681 agatcgcgaa ttgtcaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcaga
     1741 ccagagataa agttggcgaa aatttcctgc aaaccaaaac cgagaccaac accgagcgcg
     1801 gcgaccagcc actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg
     1861 ccaatcagca tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt
     1921 aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc
     1981 gtggtgatga taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga
     2041 ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg
     2101 aaagcggaat ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa
     2161 attgagcgca ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca
     2221 tcgacttcaa ttgctccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgca
     2281 cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcggcgc
     2341 tggatcagca tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca
     2401 accgaggttt caagcctcgc taacagcgcc tgtgccgttg ccagataacc cacagccgac
     2461 gccagaatgg caaccaacgg cgcgccaatc atcatgttcc acagcatatg gttggtaatg
     2521 ttgtcgccgc tgccttcttt gttgagatac agcggaatcc cggccttttt caggctgagg
     2581 gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa gccttcccag cgaaccggag
     2641 aattcacggt cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc
     2701 ccgatgctca tcaggtagta gcgcatccca cgagatacgc gttcgcgcgg ccagccaaaa
     2761 tgagcgataa acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc
     2821 agcggcacgg tggccgttac accatcgcca atcgcgaccg ccagcggata aggccacgcc
     2881 tcgcgcaagc cgtaacccag cgtcatccac agcaccggta acggtgacgc gacgagaatc
     2941 gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccaactttc
     3001 gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc
     3061 agaatcaacg cgccaaacag cggcaaaatt gtctctttgc tggtcagcat catcacactg
     3121 gctttgccca actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt
     3181 tccagcggcc aggcgatggt catcggacgc acgtcagagg tccagaacag atagcggtgc
     3241 gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc cgttggagac tttcagcttg
     3301 gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga
     3361 gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc
     3421 tgatggattt gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc
     3481 acacgcaact gcgccatttc ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt
     3541 gccacctgcg ctcgcaacgc ttcaccgagc agattggacg atcccagcca ttgcgactgt
     3601 tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt
     3661 tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg
     3721 ttaattttga attgcgcgac gatatctttc ggcaaatcgg cactgttttc tgccagtaat
     3781 tcggtacttt ccagcgcccg ctccgcctca agctgacgtt ggctattcaa ctgattacgt
     3841 aaggcctgca aatacgcatc cagttgctgg ctctcttttt ccgcaagctc tgagcgtaag
     3901 cgcgctaatt cttggcggtt attggcagac agctgcgcca gctccagttc atcaacgagc
     3961 gccttaagac gtgcagagtc agactgcaac gcgaaatttt gtgcctgatt gagcggagta
     4021 ttgccggtaa gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt
     4081 tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc
     4141 tgggcctgac ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc
     4201 gcgtcggtag acattcccgg cgacacgctg cgcggcttgt cacgcatgtt gtttaattgt
     4261 gcgcgcagag tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg
     4321 cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg cagactgaag cgcctctacg
     4381 acttccggct gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg
     4441 ctatcggggg ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg
     4501 ataatcaggc gcacgtcagc gtttcctttg atggattaga cctggtcttt tttgtcgtca
     4561 accaatgggc tggcgtcgtg ttctgcttcg atctcttcag caggaagcgg ggcaggttca
     4621 gcgtctggcg taacaaaggt ttcggtagat actgccagcg gctggcaaat tttcgtgaca
     4681 gacaggcttt ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg
     4741 gtggaaccga gtttaaagcg acccatttcc tggcctttca gcagtgccac agaaccgtcg
     4801 ttttccccgg caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg
     4861 cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt
     4921 gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc
     4981 acgttctgag ccgtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca
     5041 cgcagaatac cgttgcacgg catgtgtacg cggtggtagt cacgcgggga gaggtaagtg
     5101 gtcacaaacg taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct
     5161 tcgaggctgt agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac
     5221 tggctgataa cgccatcggc aggcatgacc agtacattcg gatcggtatc gattgggcgt
     5281 acttcgtcac gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc
     5341 ggcttttgcg cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt
     5401 tttgtcaacc atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt
     5461 ttcggcagaa tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca
     5521 ttgttttgtc gttcctgatt cggcctacat gccggatcct gaaaaaaagg ggacgattct
     5581 aacgacggtt agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc
     5641 tgcgccatgc tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct
     5701 tcctcaaccg cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat
     5761 ttacacagac ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc
     5821 aggtgccaga ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg
     5881 aagtgataca gccgagcggc ggtcgtggtg tgctgaccaa gccccgagtt gtcagagaca
     5941 tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt tcagcaggct ggatttgccg
     6001 acgccagact gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt
     6061 agcccatcct gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata
     6121 tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc
     6181 acaataatcg gctcaatctg caaggtttcg caggccacta ggtaacggtc gataatattg
     6241 agcgacagct ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata
     6301 ggttttacgc cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct
     6361 tccacgatcc ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg
     6421 cggtcgccgg ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca
     6481 tcggcggatt ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc
     6541 tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg
     6601 tgattggcgt tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtact
     6661 gactcctggt cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat
     6721 agtcactgtg aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc
     6781 gatcttgaga tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg
     6841 gtgaccgatg ccaatctgaa tattctggca gaagggccga ccattgcagt acatcagtcc
     6901 gatgaacagc tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg
     6961 gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa
     7021 ttcttaaaac agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggacag
     7081 gaccgtcgtt tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat
     7141 ctcgatgtca gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt
     7201 tttaccaagc aggggacgca tcaggcaatg gatgatatcc gtgaatcggt ggcggagctg
     7261 gcttactacc gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg
     7321 aatccacttg cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc
     7381 acttgagata aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg
     7441 tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag
     7501 tacgcgggaa tagctcagtt ggtagagcac gaccttgcca aggtcggggt cgcgagttcg
     7561 agtctcgttt cccgctccaa aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca
     7621 gtatttcgaa ttgcgacaag gcggcaactg aacgaatcct tgggaactta caaaagtatg
     7681 tgactaaggt gagtgaaggc agccaaggca gtagcggttc gaaagacgaa gat
//
LOCUS       DAJTXQ010000171         7707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000171 DAJTXQ010000000
VERSION     DAJTXQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.171"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO515_003718"
     CDS             complement(<1..304)
                     /locus_tag="OO515_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577546.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="OO515_003719"
     CDS             complement(1125..1337)
                     /locus_tag="OO515_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCR6577547.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="OO515_003720"
     CDS             complement(1413..2030)
                     /locus_tag="OO515_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6577548.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="OO515_003721"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="OO515_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6577549.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="OO515_003722"
     CDS             complement(3911..4972)
                     /locus_tag="OO515_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6577550.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /locus_tag="OO515_003723"
     CDS             5214..7316
                     /locus_tag="OO515_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6577551.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7707)
                     /gene="mcbR"
                     /locus_tag="OO515_003724"
     CDS             complement(7352..>7707)
                     /gene="mcbR"
                     /locus_tag="OO515_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6577552.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
BASE COUNT         1964 a         1801 c         1855 g         2087 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agacccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtgtgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tcgaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgccta gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 ccccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtga
//
LOCUS       DAJTXQ010000172         7667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000172 DAJTXQ010000000
VERSION     DAJTXQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.172"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..493
                     /locus_tag="OO515_003725"
     CDS             248..493
                     /locus_tag="OO515_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577553.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            490..912
                     /locus_tag="OO515_003726"
     CDS             490..912
                     /locus_tag="OO515_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6577554.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1572..3439
                     /locus_tag="OO515_003727"
                     /pseudo
     CDS             1572..3439
                     /locus_tag="OO515_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            3698..3979
                     /locus_tag="OO515_003728"
     CDS             3698..3979
                     /locus_tag="OO515_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577555.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4619..4939
                     /gene="clpS"
                     /locus_tag="OO515_003729"
     CDS             4619..4939
                     /gene="clpS"
                     /locus_tag="OO515_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6577556.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            4970..7246
                     /gene="clpA"
                     /locus_tag="OO515_003730"
     CDS             4970..7246
                     /gene="clpA"
                     /locus_tag="OO515_003730"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6577557.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         2104 a         1681 c         2100 g         1782 t
ORIGIN      
        1 aatgtctttt atatcagtat attatagggt gaacagttat ttatgaactg ttcaccaaac
       61 tattcactgt tcaccttttt gattctttat tgagctttaa gggtgaacag tggtgaacag
      121 ttggtgaata gtttttgtga aactgttaac ccattaacat catgaataaa aagggaaaat
      181 tgcaaaaggt gaacaggtga agggttaaaa tgcaaaaatt ttattttatt gctgtgaggt
      241 aaagcctgtg acaacgaagc acgccaaaaa accacaatct cacgcccttg atttgacaga
      301 acactggctg agggtggcga taaaaatcat cgaccgcaac gccggggaag gatacgcgaa
      361 agcacatccc gaactgataa gcgcattcat gaccacgacg gcggcaaact ttgccacgct
      421 gacagaacgg gagattgccg aagcggaaca ggtgacaacc atcaacgtta aaaccggaga
      481 gcagacagca tgacagcaca gatagcggct tacgggcggc tggtggctga cccgcagtta
      541 aagaccacca gcaagggtac gcaaatgacg atggcgagta tggcggttcc cctgccgtgc
      601 agccaggcag atgacggaac ggcgacgata tggttatccg tcctggcgtt tggcagacag
      661 gctgacgcac tggcaaagca ccagaaaggc gagctggtga gcgtggcggg taacatgcag
      721 gtaagccagt ggacaggcca gaacggagaa acgcggcagg gctggcaggt tatcgcagac
      781 agtgtaatca gtgccagaac ggcgcgaccg ggcggtaaaa aaggccaaca ggggcaggcc
      841 actgatgcac tgaacagggc caaacaacag gcggggaatg atgatccgta cggggataac
      901 ataccgtttt aagcaacgag tgacagaagc cggagcaatc cggctttttt gtaggtactc
      961 ctggtggggg tggcctgtcc acggggcggg cggttcgcgg aaaaaggcgt attttttgat
     1021 ttttatgtca ccatcaccac cagtttaagt tattgatata tggcaaaata aaaattttta
     1081 gtgtcgaatt aggttgtttt ttgttcgacg ccggaacgtt cccgaaagca tttacaaaaa
     1141 acaggcgcaa aaaaaagcgc cccgatggtg ccaccggagg cgctttgtca gcgacaaagg
     1201 atactgccac taccgaaatg atgagtctta attgtgctgg cgtggcaata attagtcaat
     1261 tcttttgctg gtatgacaaa aatcagaaaa cacataatct gatttttata tagaaaatga
     1321 tttatttacc agttttatcg atcaataatg atgcccgtta atcataacgg aggcagactt
     1381 atgccagaga acaacagcag aaaaccggat aaaagtgcaa cagtgcacat agacgccgga
     1441 actatggaga agctcgaacg ctatcagcag ttcattaaaa gtcatcaccc tggaatgcca
     1501 gtacccacga aagggcagat tgcgagaagc gcggttgaat actggtacag ggcaacgtta
     1561 ggagcctggc tatgaaaaca tggttttcca ttaaggctat ggctgatgtt gtacatgtac
     1621 gcatttatga cgagatcggc gggtacggtg taaaagcatc ggcacttact gacgagatca
     1681 acgcgtgcgg taatgtgcct gaaatccatc ttcgcatcca ttcacctggt ggcgacatct
     1741 ttgaagggct ggctatctat aacgccctga aaaatcatcc ggcaaagaaa attgtacaca
     1801 ttgaaggcat ggcggcttct atggcctcgt ttattgccat gtgtggcgat cacatcgtta
     1861 tgcctgaaaa cgcgatgatg atgatacatg ccccccgtgg tgttactgcc ggagtgtcgg
     1921 gcgacgttcg ccgctttgct gacctgatgg acaagctggg cgacacgatg gcggaaacct
     1981 acgccggaag aacgggcagg agcaaacagg aaatcaccgc catgatggag gcggaaacct
     2041 ggatggatgg caatgagtgt aaggctaatg gcttcgcaga tgaggttata cccgcgatta
     2101 cagcaatggc cagaattgaa tcaaaacgaa tcggagattt ttcgaatatg ccggaaaata
     2161 ttaaaagcat gatcagccag aaaactggca gcggagaaca ggaacgactc aacgggatac
     2221 gtgaattgtt tggcacgttc aatggaagat ataacgacct ggctataagt tgtcttgctg
     2281 attcaggctg tagcgttgag gatgcacgcg aacgcctttt actcactatg ggtaaagaat
     2341 cgacaccgac aaacaaaacc acccccgcaa atctttacta cgcgtacacg gataacggca
     2401 acataaccgg cgatgcaatg cgtcaggggc ttaatgcgcg tctgggacat gaacaggcag
     2461 aacgcggcaa cccttacgcc atgatgagcc ttttcgatat ggcacaggca tcattaaccc
     2521 atcgcggcat aagcacaggc agctacagca cacgctcgca gatagtaaac gcggcattca
     2581 cccacagcag cagcgatttt acttatatcc ttgctggtgg tgctgaaaaa tccgtgaaca
     2641 cagcggcgaa acgttccgcc agtggacgaa aaaaggttca ttgtcaaact tccatgaggc
     2701 acgtcgtgtt ggcctgaatg gcttctctac attaaacaaa gtgccggaag gcgcggaata
     2761 taaatacatc accaccagtg accgtggcga accaatcgcg ctggcaactt acggaaatat
     2821 tttcagcatt acccgacagg cgattattaa cgatgacctg aatcagttaa cagttatccc
     2881 acaggcaatg gggcgagcag ccgccagaac ggtgggtaat ctggttcatc tggtgctgac
     2941 aggaaacaca aagctttctg acggtatacc gctgttcagc aaaaaacacg gaaatgatat
     3001 tgataacgga gtcagcacta cagggcttgg cattgcacgc cgtgcaatgc gcacacagag
     3061 ggatgaaaac ggtgaggtgc tgaatattgt gccaaaattc cttcttgttc ctgcggctct
     3121 ggaagacaga gcgttgcaaa tggttaactc aaccacgcct ttcggtgccg atacaaacag
     3181 tggttcatat aacccgtacc acaaacaact ggatattatt gtcgatcccc gccttgatga
     3241 agtcagtgat aagcaatggt acatgctttc agcacaggga acggacacaa ttgaggtggc
     3301 ttatcttgac ggtaatgacg agccttacct tgagcagcag gagggcttta ttgttgacgg
     3361 tgtggcctgg aaggtccgca ttgatgctgg cgttgctgct ctggattatc gcggaatggt
     3421 cagagcgatt tcttcatgag aacaaggcgg cagcagccgc cttttttgcg ggtcctcctg
     3481 gtgggggggg cctgtccacg gggcgggtcg gcgcggaaaa aagcgcattt ttgtggtttt
     3541 atcgtcatca tcatcatgtg tgtaacctgt tgtttttaat gtagttgatg caaaaaagat
     3601 gatgattgtg gttaattttt gttcgacatc ttttagcgtg acagattctt tacaaaaaat
     3661 ctgagcttgt tttcttaacc agcgcgatgg gggcacaatg acagaagccg aaatactggg
     3721 attaatccgc cgcgtcgccg gaatcagcca gcaggttgac gaacaggcca tgcaaccgga
     3781 cagcgtgacc gctgaaaatt atgcgcgtgt ggtggatgag gtaatgcgcc gtgacggtat
     3841 tgagcttaac ggcgtggata tgcgcaacat acgaaccaga gttcttgagt tgctggcata
     3901 ccgtcgccgt tctcaacaac ggagggagag cgcgaaaaat acttaccagt ggaggaagcc
     3961 ggagcactta cggcggtaac tggttgatat taccgaaagt gcaaaaatgc acttagcaac
     4021 tggtggcgag ttgcagatct gcaactcgac tatgaaacta cggaaactac ccgtagtttg
     4081 ggtagtaaga gtaacaccca gattttgggg cttactcgcg atacccaaat aaagggtatc
     4141 ggtggaagaa atatcgtttc tcatatgtga gtaccgaggg cggaattccg ccttcggtta
     4201 cttattgtgc gcatgcacag ggaggggcgg gtcaaatctc tgtaccctga cgtcttccgg
     4261 actgccagcc ccatcgattt tttatacccg cgaaaaatga aaaacgcttc acgctggtgg
     4321 gcctgatgcc gataactggt ttcatgactg atagccggaa aatgataacg attaaagcgt
     4381 gttgatcttt gttcgtggtt gttcgatatt gttcgtggaa cggtgtagtt aatggtgtag
     4441 ttaattttac tgtttttgac gagcttattg tttgcaactc aatgaaaaat aaaggctaat
     4501 aagccaaatg ctataaatga tagttatcta tcatgtggag tagattggtc aggcaaataa
     4561 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
     4621 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
     4681 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga
     4741 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
     4801 gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc
     4861 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
     4921 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
     4981 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     5041 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     5101 ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     5161 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt
     5221 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac
     5281 cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt
     5341 gcgtaaacat gaagtcagcc gtctcgatgt ggtgaatttt atctctcatg gcacgcgtaa
     5401 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     5461 tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg
     5521 aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg
     5581 tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc
     5641 ggaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac
     5701 gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga
     5761 aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat
     5821 tgatgagatc cacaccatta tcggtgcggg ggcagcgtct ggtggtcagg ttgatgcggc
     5881 taacctaatc aaaccgttgc tctccagcgg taaaattcgt gtaattggtt cgacaaccta
     5941 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
     6001 tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
     6061 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct
     6121 ggcggtgaaa tacatcaatg atcgtcatct gccggataaa gcgattgatg ttatcgacga
     6181 agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
     6241 ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag
     6301 tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga
     6361 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
     6421 acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt
     6481 gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta
     6541 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
     6601 tcagggcggt ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga
     6661 cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg
     6721 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac
     6781 caccaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa
     6841 cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct
     6901 cgacaacatt atctggtttg atcatctgtc aaccgacgtg atccatcagg tggtggataa
     6961 attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca
     7021 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg ctcgtccgat
     7081 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc
     7141 gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta
     7201 cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatcta attgttaggt
     7261 aggttggtga agtccgtaat ctcgaaagag gttacggact ttttgtttat ggggtggaac
     7321 ggtgaaaacc ctatttttgg aggtgaaggt aatttgttga taattagtgc tgctggaaaa
     7381 ggtaaggata aaaaagggtg acagcaggaa aatgagatgg ttttgcttta ttaacaacgg
     7441 actaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatc gacggggtgc
     7501 ggtaaaacct ttgcgaacgc gttcactgcc gtacaggcag cttagaaatt cacaggtaac
     7561 atactccacc cgcccaccat gttcactgcc gtacagaaag ataaaatgcg aaaaaaaagc
     7621 tcgcactttc gtacgagctc ttctttaaat atggcggtga ggggggg
//
LOCUS       DAJTXQ010000173         7623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000173 DAJTXQ010000000
VERSION     DAJTXQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.173"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..96
                     /locus_tag="OO515_003731"
     CDS             <1..96
                     /locus_tag="OO515_003731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6577558.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OO515_003732"
     CDS             complement(152..487)
                     /locus_tag="OO515_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6577559.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OO515_003733"
     CDS             complement(491..835)
                     /locus_tag="OO515_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6577560.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OO515_003734"
     CDS             complement(837..1010)
                     /locus_tag="OO515_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577561.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OO515_003735"
     CDS             1111..1296
                     /locus_tag="OO515_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577562.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OO515_003736"
     CDS             1257..1376
                     /locus_tag="OO515_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6577563.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO515_003737"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO515_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6577564.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO515_003738"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO515_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6577565.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO515_003739"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO515_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6577566.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OO515_003740"
     CDS             4176..4544
                     /locus_tag="OO515_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6577567.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OO515_003741"
     CDS             complement(4652..5053)
                     /locus_tag="OO515_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6577568.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OO515_003742"
     CDS             5295..5588
                     /locus_tag="OO515_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6577569.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OO515_003743"
     CDS             5660..6319
                     /locus_tag="OO515_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6577570.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OO515_003744"
     CDS             6411..6857
                     /locus_tag="OO515_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6577571.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OO515_003745"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OO515_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6577572.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAJTXQ010000174         7491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000174 DAJTXQ010000000
VERSION     DAJTXQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.174"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OO515_003746"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OO515_003746"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6577573.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OO515_003747"
     tRNA            647..722
                     /locus_tag="OO515_003747"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO515_003748"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO515_003748"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6577574.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OO515_003749"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OO515_003749"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6577575.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO515_003750"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO515_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6577576.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OO515_003751"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OO515_003751"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6577577.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OO515_003752"
     CDS             complement(5917..6249)
                     /locus_tag="OO515_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6577578.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OO515_003753"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OO515_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6577579.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1825 a         2029 c         1868 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAJTXQ010000175         7428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000175 DAJTXQ010000000
VERSION     DAJTXQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.175"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..98)
                     /locus_tag="OO515_003754"
     CDS             complement(<1..98)
                     /locus_tag="OO515_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR6577580.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
     gene            complement(123..1223)
                     /locus_tag="OO515_003755"
     CDS             complement(123..1223)
                     /locus_tag="OO515_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6577581.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="OO515_003756"
     CDS             complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="OO515_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6577582.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2371..2937)
                     /locus_tag="OO515_003757"
     CDS             complement(2371..2937)
                     /locus_tag="OO515_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6577583.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3153..4889
                     /gene="argS"
                     /locus_tag="OO515_003758"
     CDS             3153..4889
                     /gene="argS"
                     /locus_tag="OO515_003758"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6577584.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5066..5428
                     /locus_tag="OO515_003759"
                     /pseudo
     CDS             5066..5428
                     /locus_tag="OO515_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5432..6502)
                     /locus_tag="OO515_003760"
                     /pseudo
     CDS             complement(5432..6502)
                     /locus_tag="OO515_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="OO515_003761"
     CDS             complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="OO515_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCR6577585.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1946 c         1776 g         1786 t
ORIGIN      
        1 tcttctgccc atgcggggag cggtgctgcc gatgtcacca cctgcgctcc agcggcaata
       61 ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag
      121 gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc
      181 agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca
      241 ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca
      301 agtttttcag cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca
      361 ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt
      421 accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc
      481 gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc
      541 tgtttgccac tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg
      601 cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc
      661 tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca
      721 atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc
      781 tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac
      841 gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga
      901 gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta
      961 gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca
     1021 cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa
     1081 gatttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct
     1141 ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac
     1201 ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca ccggaatcat
     1261 tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa
     1321 ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca
     1381 gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga
     1441 atataaaaaa tattattagc acaccgctat gttcactgtc acccgctcaa cagatcacgg
     1501 ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg
     1561 gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc
     1621 tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc
     1681 gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc
     1741 ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag
     1801 tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg
     1861 gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc
     1921 agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac
     1981 atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc
     2041 attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa
     2101 cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag
     2161 caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc
     2221 acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat
     2281 tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct gtaacagcaa
     2341 atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg caacgatctc
     2401 ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg
     2461 attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga tccccggtag
     2521 gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc
     2581 cagcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca
     2641 cggtagctcc acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa
     2701 cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa
     2761 cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc
     2821 agtcagcgga gtgatattca accccagacg gagggaaagt tcatctaatg cgtgggtaaa
     2881 tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat
     2941 cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc aaccaaccgc
     3001 tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct
     3061 gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat
     3121 ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa
     3181 aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc
     3241 attcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa
     3301 aactgggtat gacaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg
     3361 gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg
     3421 cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gtttctacgc
     3481 cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcatg
     3541 tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg
     3601 gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga
     3661 ttgcatggct ggaaaagcag cagcaggaaa acgccggtga tgaaatggag ctggctgacc
     3721 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     3781 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     3841 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
     3901 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     3961 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     4021 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     4081 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     4141 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     4201 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     4261 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     4321 aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg gcagaaaaga
     4381 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     4441 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     4501 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     4561 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     4621 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     4681 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     4741 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     4801 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
     4861 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
     4921 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
     4981 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
     5041 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
     5101 ctcttgccag gcttacgcag aagatacggt tatagaaaac gaccatgaca tttcagccat
     5161 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     5221 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
     5281 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg aacaacttaa
     5341 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
     5401 cctttcaaat gaggacgttc aggatttaat gtacatcgtt ccacgtcgct tcgacaacgg
     5461 tattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc
     5521 gtatccacca ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca
     5581 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     5641 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     5701 cgatgacccc cgcggcggta aaaatcacca gcgccggaat ttgtgccacc agaccatcgc
     5761 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     5821 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     5881 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca
     5941 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt
     6001 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     6061 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca
     6121 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc
     6181 ctgccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa
     6241 gtgccagacg caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg
     6301 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     6361 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     6421 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca
     6481 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     6541 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     6601 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     6661 ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     6721 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     6781 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     6841 ttattgacaa tgacatcagc tttcggcaca tcggccatca tccgacgccg tgcagcagct
     6901 cgctgcatct gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca
     6961 tcacgaatat cctgccgcga catacgcagc tttttcaggt ggctgaagat ttggaaaaag
     7021 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagcccaacc
     7081 agatccatcg cattacccat ggcggtagtc ggagactcgg ccattaagcg catcatctgc
     7141 ggccagtgat gccagagata aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     7201 gccttaagca actccgcgcc agtctgtgcc gagaacatcc gtttaatgcc cggtagcggg
     7261 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc ccccagcatg
     7321 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg
     7381 gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtca
//
LOCUS       DAJTXQ010000176         7300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000176 DAJTXQ010000000
VERSION     DAJTXQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.176"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            163..317
                     /locus_tag="OO515_003762"
                     /pseudo
     CDS             163..317
                     /locus_tag="OO515_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OO515_003763"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OO515_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR6577586.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="OO515_003764"
     CDS             858..2264
                     /locus_tag="OO515_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6577587.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="OO515_003765"
     CDS             2509..3654
                     /locus_tag="OO515_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6577588.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="OO515_003766"
     CDS             3672..4685
                     /locus_tag="OO515_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6577589.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="OO515_003767"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="OO515_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="OO515_003768"
     CDS             5576..6370
                     /locus_tag="OO515_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6577590.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..>7300
                     /locus_tag="OO515_003769"
     CDS             6392..>7300
                     /locus_tag="OO515_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6577591.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAA"
BASE COUNT         1678 a         1757 c         2004 g         1861 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg
//
LOCUS       DAJTXQ010000177         7293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000177 DAJTXQ010000000
VERSION     DAJTXQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.177"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..219
                     /locus_tag="OO515_003770"
     CDS             <1..219
                     /locus_tag="OO515_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6577592.1"
                     /translation="DICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFK
                     DPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            470..2032
                     /gene="hpaB"
                     /locus_tag="OO515_003771"
     CDS             470..2032
                     /gene="hpaB"
                     /locus_tag="OO515_003771"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6577593.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2050..2562
                     /locus_tag="OO515_003772"
     CDS             2050..2562
                     /locus_tag="OO515_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6577594.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            2955..5105
                     /gene="btsT"
                     /locus_tag="OO515_003773"
     CDS             2955..5105
                     /gene="btsT"
                     /locus_tag="OO515_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6577595.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5155..5358
                     /locus_tag="OO515_003774"
     CDS             5155..5358
                     /locus_tag="OO515_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6577596.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5369..6325
                     /gene="yjiA"
                     /locus_tag="OO515_003775"
     CDS             5369..6325
                     /gene="yjiA"
                     /locus_tag="OO515_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6577597.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6403..>7293)
                     /locus_tag="OO515_003776"
     CDS             complement(6403..>7293)
                     /locus_tag="OO515_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6577598.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         1680 a         1988 c         1907 g         1718 t
ORIGIN      
        1 gacatctgcc gccgctttgc caaccgtccg ccaaaacggt tgatttttga caggcagctg
       61 cgagaagcca agcggctgct gctgttttct gataacgccg tgaacaatat tgcctggcaa
      121 ctcggtttta aggatccagc ttattttgcg cgctttttta atcgcttagt cggttgctcg
      181 cccagtgctt atcgtgccaa aaaagtacct gtgacgtgaa aatcccttag ccttaacggg
      241 aaaccaggca ccacctgcta ttcccctttc tccgcctgga gcaaggggat ttagtaacgt
      301 caaaaatcgc taaaagcgac cgcgatcaca aaaccgtacc cgacctgaaa agtaccagcc
      361 attgtcccaa aagtgtcttc ccgtaatcgc ccctgcaagc ttcaattgaa taacaaaaaa
      421 aacacacaaa attaacaatc gtattccgat taatactgta gaggtcgaca tgaaaccaga
      481 agatttccgc gccagtaccc aacgtccgtt caccggggaa gagtatctga aaagcctgca
      541 ggatggtcgc gagatctata tctatggcga gcgagtgaaa gacgtcacta ctcatccggc
      601 atttcgtaat gcggcagcgt ctgttgccca actgtacgac gcgctgcaca aaccggagat
      661 gcaggactct ctgtgctgga acaccgacac cggcagcggc ggctataccc ataaattctt
      721 ccgcgtggcg aaaagtgccg acgacctgcg ccagcaacgt gacgccatcg ctgagtggtc
      781 acgcctgagc tatggctgga tgggccgtac cccagactac aaagctgctt tcggttgcgc
      841 actgggcgcg aatccgggct tttacggtca gttcgagcag aacgcccgta actggtacac
      901 ccgtattcag gaaactggcc tctactttaa ccacgcgatt gttaacccac cgatcgatcg
      961 tcatttgccg accgataaag tgaaagacgt ttacatcaag ctggaaaaag agactgacgc
     1021 cgggattatc gttagcggtg cgaaagtggt tgccaccaac tcggcgctga ctcactacaa
     1081 catgattggc ttcggctcgg cacaagtaat gggcgaaaac ccggacttcg cactgatgtt
     1141 cgttgcgcca atggatgcca atggcgtcaa attaatctcc cgcgcctctt atgagatggt
     1201 cgcgggtgct accggctcgc cgtacgacta cccgctctcc agccgcttcg atgagaacga
     1261 tgcgattctg gtgatggata acgtgctgat cccatgggaa aacgtactga tctaccgcga
     1321 tttcgatcgc tgccgtcgct ggacaatgga aggcggtttt gcccgtatgt atccgctgca
     1381 agcctgtgtg cgcctggcag tgaaactcga cttcattaca gcactgctga aaaaatcact
     1441 cgaatgtacc ggcaccctgg agttccgtgg tgtacaggcc gatctcggtg aagtggtggc
     1501 gtggcgcaac accttctggg cattgagtga ctcgatgtgt tctgaagcga cgccgtgggt
     1561 caacggggct tatttaccgg atcatgccac actgcaaacc tatcgcgtac tggcaccaat
     1621 ggcctacgcg aagatcaaaa acattatcga acgcaacgtt accagtggcc tgatctatct
     1681 cccttccagt gcccgtgacc tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt
     1741 gcgcggttcg aatggtatgg atcacgtcca gcgcatcaag atcctcaaac tgatgtggga
     1801 cgccattggc agcgagtttg gtggtcgcca cgaactgtat gaaatcaact actccggtag
     1861 ccaggatgag attcgcctac agtgtctgcg ccaggcacaa agctccggca atatggacaa
     1921 gatgatggcg atggttgatc gctgcctgtc ggaatacgac cagaacggct ggactgtgcc
     1981 gcacttgcac aacaacgacg atatcaacat gctggataag ctgctgaaat aacgcagcag
     2041 gaggttaaga tgcaattaga tgaacagcgc ctgcgctttc gtgacgcgat ggccagcctg
     2101 tcggcagcgg taaatattat caccaccgag ggcgacgccg gacaatgcgg gattacggca
     2161 acggccgtct gctcggtcac ggatacacca ccatcgctga tggtgtgcat taacgccaac
     2221 agtgcgatga acccggtttt tcagggcaac ggtaagttgt gcgtcaacgt cctcaaccat
     2281 gagcaggaac tgatggcacg ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt
     2341 tttagcctct catgctggca aaaaggtccg ctggcgcagc cggtgctgaa aggttcgctg
     2401 gccagtcttg aaggtgagat ccgcgatgtg caggcaattg gcacacatct ggtgtatctg
     2461 gtggagatta aaaacatcat cctcagtgca gaaggtcacg gacttatcta ctttaaacgc
     2521 cgtttccatc cggtgatgct ggaaatggaa gctgcgattt aagctccagc cccttcacgc
     2581 attcgtcgaa ggggcatttt tattgtgatc tcaatcacat atccacccga tgaataattc
     2641 ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc ctaaaataac cactcagtta
     2701 tttaccttac tttacgcgcg cgtaactctg gcaacatcac tgcaggatag cggtcaattt
     2761 acctcctcaa acgcaacgca aacctagaac ggcttcggcc tatgattaac aaattactct
     2821 atgaattttg gtttgcagga aggcagcaag tgagtgaatc cccgggagct tacaacagta
     2881 agtgacaggg gtgaacgaac gcaactgccg cacctgtaag ccaaaagacg acgagtaaaa
     2941 tgccaggttt tactatggat acgaaaaaac tattcaagca cataccctgg gtgattctcg
     3001 gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg
     3061 ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc
     3121 tgtacatcgc ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta
     3181 acaacgacgg tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg
     3241 ccgctatcgc cggtgctggt ccgctggtcg gtccggttct cgccgcacag atgggccacc
     3301 tgcctggcac gctgtggctg ctggcggggg tagtactggc cggtgcggtt caggacttta
     3361 tggtgctgtt tatctcctct cgccgtaacg gcgcatctct cggtgagatg ataaaagaag
     3421 agatgggacc agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca
     3481 tccttgccgt cctggcgctg attgtggtta aagccctggc cgaaagtccg tggggtgtct
     3541 tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg tatctacatg cgctttatcc
     3601 gtccggggcg tgtgggtgaa gtctctgtca ttggtatcgt gctgctggtt gcctctatct
     3661 acttcggtgg cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca
     3721 ccaccattac cttcgcgctg attggctatg cgtttgtttc cgcattgctg ccggtgtggc
     3781 tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc
     3841 tggcgctggg tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca
     3901 ttgacggtac tggcccgctg tggaaaggtg ctctgttccc gttcttgttc attaccatcg
     3961 cctgtggtgc ggtatctggc ttccacgcac tgatctcttc cggtacgacg ccgaaactgc
     4021 tggctaatga aaccgacgcg cgtttcattg gctacggcgc aatgctgatg gagtccttcg
     4081 tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc gggtctttac ttcgcgatga
     4141 acaccccgcc tgctggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga
     4201 acgcgccgat catcatggcg cagctgaaag acgttaccgc acacgcggca gcgaccgtca
     4261 gctcttgggg cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg
     4321 agccttctgt cctgaaccgt gcaggtggtg cgccaacgct ggcggtaggt atcgctcacg
     4381 tgttccacaa agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt
     4441 tcgaagccct gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgttttatgc
     4501 tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg
     4561 gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg
     4621 tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc
     4681 tggcagccgt agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat
     4741 acatctgggt aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg
     4801 gtctgaaact gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt
     4861 acaaagagaa gattgctaac ggtactgacc tgacggcgca gcagattgcc aacatgaacc
     4921 acatcgttgt gaacaactac accaacgcag gcctgagtat tctgttcctg attgtggtgt
     4981 acagcatcat cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta
     5041 ctgacaaaga aactccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc
     5101 actaaccgtg tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt
     5161 ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggcatt
     5221 ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg
     5281 agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc
     5341 ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg
     5401 ttttttaggc gcgggcaaaa ccaccctgct gcgccatatt cttaacgaac aacacggcta
     5461 cgagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc aactgattgg
     5521 cgaccgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa
     5581 cgagctggaa gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt
     5641 cgaccgtctg gtcattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt
     5701 cttctcccat gagattttat gccagcgcta cctgctggac ggggtgattg cgctggtgga
     5761 tgcggtacat gccgatgagc agatgaacca gttcaccatc gcccagtccc aggttggcta
     5821 cgccgaccgt attctgctga cgaaaaccga cgtcgcaggc gaagcagaga aactgcgtga
     5881 acgcctggcg cgcatcaacg cccgcgcacc ggtctacacc gttacccacg gcgatatcga
     5941 cctgggcctt ctgttcaaca ccaacggttt tatgctggaa gaaaacgtcg tcagcaccaa
     6001 accgcgtttc cactttatcg cggacaaaca aaacgatatt tcgtcgattg tggtggaact
     6061 ggattacccg gtagatatca gcgaagtttc ccgggtgatg gaaaacctgc tgctggaatc
     6121 ggcggataaa ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct
     6181 gctgttccag ggcgtccagc gcctctacag cgccgactgg gaccgcccgt ggggcgatga
     6241 aaaaccgcat agcacgatgg tgtttatcgg gattcagttg ccggaggatg agattcgggc
     6301 ggcgtttgcg ggactcaaaa agtaaccagc cctccaacat cacaatacct gagactttat
     6361 caccctcata ttcctccaga atatgagggc attcgggctt aattatctct gaacgtgcaa
     6421 cacttcctgc gactccttat gcgcctccac atcctgcttc gttaaccaga gcgacttacg
     6481 accaaaattt tcgtacattt tcagctgatc ttcatagtgc tgatcaactg ttccatcggg
     6541 agcaataaac ccactctgac cgggtgcgac cacatcccag gcaagcaccg gacgatcgct
     6601 tgtcgttggt gagaaaacaa tcatatcgtt ttctgttcca cggttttgat actccgcctg
     6661 atgacgcgtt tcttccgctg cggcctgcgg tacaccaaag aaattatttg cacggaacgt
     6721 taaggccatt gcaggtgttt tccagttact cacattattg ccatagcgtt tggaaagagt
     6781 ctcccaggta tcttccagcg cagccaacac aacctcctgc tgtggtttcc cagcaaacag
     6841 atcaaccgcc tgtgggattg gtgatttgtc tccctgcacc gcctcataca aaatttttgc
     6901 tccaacactt atattcagcg aaccagttgg gccgtcctgg gttgtttcgt agccactggc
     6961 gctgtaccac ttatcaaatg gcataggtac ggcagccact acggtacgct tcaacatact
     7021 ggtcagccaa acgttcagga tggcagagcc tggctgctgc caggttttac catcatcatt
     7081 aagcaaattg atgccatccc aacgtgttaa tgtatccacc aactgacggc gcggatcgct
     7141 ctgtgtcaaa ccagatgtcg ctgcttgcag agtaggtaaa aaaagcctca ggttaagatc
     7201 ctgacgactg gtttggcgaa taacatccca tgcctgatca gcagttaagc gtggcttttg
     7261 ctcaagcagt cggtcgatct ccgtaacgcg atc
//
LOCUS       DAJTXQ010000178         7116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000178 DAJTXQ010000000
VERSION     DAJTXQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.178"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..235
                     /gene="gatB"
                     /locus_tag="OO515_003777"
     CDS             <1..235
                     /gene="gatB"
                     /locus_tag="OO515_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6577599.1"
                     /translation="MAAEEIKELCQNHSIPVELIQCRVNEIETYMDGVHLICTTAKVD
                     RSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            239..1594
                     /locus_tag="OO515_003778"
     CDS             239..1594
                     /locus_tag="OO515_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6577600.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            1642..2682
                     /gene="gatD"
                     /locus_tag="OO515_003779"
     CDS             1642..2682
                     /gene="gatD"
                     /locus_tag="OO515_003779"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6577601.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            2788..3561
                     /locus_tag="OO515_003780"
     CDS             2788..3561
                     /locus_tag="OO515_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6577602.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(3643..4542)
                     /gene="yegS"
                     /locus_tag="OO515_003781"
     CDS             complement(3643..4542)
                     /gene="yegS"
                     /locus_tag="OO515_003781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6577603.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            4948..5265
                     /locus_tag="OO515_003782"
     CDS             4948..5265
                     /locus_tag="OO515_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577604.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(5595..6956)
                     /gene="yegQ"
                     /locus_tag="OO515_003783"
     CDS             complement(5595..6956)
                     /gene="yegQ"
                     /locus_tag="OO515_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6577605.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
BASE COUNT         1762 a         1601 c         1844 g         1909 t
ORIGIN      
        1 gatggcggcg gaagaaatta aagagttgtg tcagaatcat agtattcctg ttgaattaat
       61 ccagtgtcgg gttaatgaaa tagaaaccta tatggatggt gtgcatttga tatgcaccac
      121 tgccaaagtg gatcgtagtt ttggcgatat tccgttagtt cacggcatgc cttttatttc
      181 tggtgtcggt atcgaagcat tacaaaataa aattctgact atcttacagg ggtgacctat
      241 gttttcagaa gtcatgcgtt atattctcga cctcggccct acggtgatgc ttccgattgt
      301 catcattatt ttttctaaaa tattaggcat gaaggcaggc gattgcttta aagcgggtct
      361 gcatatcggg attggctttg ttggcattgg ccttgtgatt ggcttaatgc tggattccat
      421 tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt
      481 cggctggccg ggctcttcac caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc
      541 gattgcgatt ctggttaacg tggcgatgtt actgacccgt atgacgcggg tggtaaatgt
      601 tgatatctgg aatatctggc atatgacctt caccggcgcg ttgctgcatc tggcaaccgg
      661 ttcatggatg atagggatgg caggtgtggt aattcacgcg gcgtttgttt ataagctcgg
      721 cgactggttt gcccgcgata cccgaaattt ctttgagctg gaaggtattg ctattccgca
      781 cggtacgtcg gcgtatatgg ggccgattgc ggtgctggtc gatgctatca tcgagaaaat
      841 cccaggcgtt aaccgaatta aatttagcgc cgacgatatt cagcgcaaat ttggtccatt
      901 tggcgagcct gtcaccgtgg gttttgtgat ggggctgatt atcggcatcc tcgccggtta
      961 cgatgtcaaa ggtgtattgc agctggcggt aaaaacggcg gcagttatgc tgctgatgcc
     1021 acgggtgatt aaacccatca tggatggttt aacgcccatc gctaagcagg cccgtagtcg
     1081 tttacaggcg aagttcggcg gtcaggagtt cctgattggc ctggatcccg cattactgct
     1141 ggggcatacg gcggtggtat cggcaagcct gatttttatc ccgctcacca ttttaattgc
     1201 tgtttgtgtt ccgggtaatc aggtactgcc gtttggcgat cttgccacca tcggcttctt
     1261 cgtggcgatg gcggtcgcgg tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt
     1321 catcattatg agcatcaccc tgtggatcgc gacgcaaact attggtttgc acacccaact
     1381 ggcggctaat gctggggcgt taaaagccgg gggtatggtg gcttcaatgg atcagggcgg
     1441 ttctcccatt acctggttac tgattcaggt tttctccacg caaaatattc ccggtttcat
     1501 tattatcggc gcaatttatc ttaccggtat tttcatgacc tggcgtagag cgcgtggttt
     1561 tattaaacaa gagaaaaccg ttctcgcaga ataattttta cctgaggggg aggttatccc
     1621 ccttaacaat caggagtttt tatgaaatca atggtgaatg atactgatgg tatcgtgcgc
     1681 gttgcagaaa gcgtcattcc tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc
     1741 agctcgggct tatgtggttc cgatttaccc aggatattta aaaatggtgc acattattat
     1801 ccaataacgt taggccatga atttagcggc tatattgatg cggtgggatc cggtgttgat
     1861 gatttacacc ctggcgatgc ggttgcctgt gtgccgttat taccctgttt tacttatcca
     1921 gagtgtttga aagggtttta ttcccagtgc gcaaaatatg attttattgg ctcgcggcgt
     1981 gatggtggat ttgctgaata tattgtcgtt aagcgaaaaa atgtctttgc tctacccacg
     2041 gatatgccta ttgaggatgg ggcttttatt gagccgatta ccgttggcct gcatgctttt
     2101 catttagcgc aaggttgtga gaataaaaac gttattatta ttggtgccgg aaccattggc
     2161 ctgctggcta ttcagtgcgc tgtcgcgctg ggagcaaaga gtgtgacggc gatcgacatt
     2221 agctcagaaa aactggcatt ggcaaaatct ttcggtgcga tgcaaacatt taacagtagc
     2281 gaaatgagcg cgccgcaaat gcagagcgtt ttacgcgaac tgcgctttaa tcagcttatc
     2341 ctcgagacgg ctggcgtacc gcaaactgtc gaactggcgg tagagattgc cggtcctcat
     2401 gcccaactgg cgctggtggg cacgttgcat caggatctgc atttaacatc ggcaacgttt
     2461 ggcaaaatat tgcgtaaaga gctgacggtt atcggcagtt ggatgaacta ttccagccct
     2521 tggccggggc aggagtggga aacggcgagc cggttgctga cagaacgtaa gttaagcctg
     2581 gagccattaa tcgctcaccg tggaagcttt gaaagcttca cccaggcggt gcgtgacatc
     2641 gctcgtaatg ccatgccggg caaagtgttg ctcattccat gaaaccgcgg gccagcgtga
     2701 tgctggcccg gtattgtgca aaacagatta ttcaccaatg gtcccccttc gtttacacta
     2761 gccacaattg aatatgggta aatgacgatg aattcattcg agcgaaggaa taagatcatc
     2821 caattagtga atgaacaggg aaccgtgctt gttcaggatc tggcgggagt atttgctgcc
     2881 tcggaagcga caatccgtgc cgatttgcgc tttctcgaac aaaaaggcgt ggttacgcgc
     2941 tttcatggcg gtgcggcgaa aataatgtcc ggtaatagtg aaaccgagac ccaggaagtc
     3001 gggtttaaag agcgatttca gctcgccagc gcgccaaaaa acagaatagc gcaggcggca
     3061 gtcaaaatga tccatgaagg gatgaccgtt attctcgaca gcggaagtac gacaatgctt
     3121 atcgccgaag gattaatgac cgctaaaaat atcacggtga ttaccaacag tctcccggca
     3181 gcgtttgccc tttccgaaaa taaagacatt actctggtcg tctgtggtgg cacggtccgc
     3241 cataaaacgc gctcgatgca tggttccatt gccgagcgtt cattgcagga tattaatgcc
     3301 gatttaatgt ttgtcggtgc tgatggtatt gatgcggtta atggtattac gacctttaat
     3361 gaaggttatt cgattagcgg tgcgatggtc accgccgcta ataaagtgat tgccgttctc
     3421 gattcatcga aatttaaccg tcgcggtttt aatcaggtac tgccaatcga aaaaattgac
     3481 attattatta ccgatgatgc tgtatcagag gtggataaac tggcattgca gaagacacgg
     3541 gtaaaattaa ttacggtgta gtattgttct gtatgccgga taaggtattt acaccgcatc
     3601 cggcatttcg gattctccct ggttcaagag agaatagttt gattaacgca ataatagaca
     3661 atctggtggt aatcgacaac gcaacgccgc cggaagtatt tcaatatgaa agttttgtcc
     3721 gctcaacggt tcgccatcaa gattaaaagt gatttcgtgt ggggcttgta tatcaaacca
     3781 cgacgaagcg ccttcgataa tattcgggtt atcttcgtca gattttaagg ttgatacgag
     3841 agccggaaga atttcatcgc cggtaaaaat gcgcagttgc agcaagccat cgttaattaa
     3901 cgcgttcgga cacaattgct gaccgccacc ggcctgacgc ccgttaccaa taccaatgac
     3961 cagggcgtca ccttgccagt gaaagttttc accgcggatt tcacaacggt ccggttgcag
     4021 agtatccatg cgcattaagc catgaatgat gtaagagacg ccacccagcg cggcttttaa
     4081 tttttccggc gtttctgtgg taatacgcgt cccaaatccg cctgtcgcca tattaataaa
     4141 acaggtttgt ttgttgacct gcgccatatc tatcgcaatg gcgttaccgg caattgccag
     4201 tttcagcgcc ttatccagtg cctcaggaat ccctacactg gtggcaaaat cattggcggt
     4261 tcctaatggc aaaattccca gcgcgggtat gccatccccc tcacactgaa tcaacgccgt
     4321 agaaacttca ttaatggtgc catcgccacc accggcaatc accgttgcga cgcccaactt
     4381 ccgggcctcc tctacatatc gtgcggcatc gcctttctcc caggtgaccc gcacatggat
     4441 cgtcattcct tcctcacgca acagcataat tgcttcgcgc aagggtagat tgtcagtact
     4501 tttgccatta agaatcagta agctggcggg aaattctgcc atgatcgtat gtgcctttat
     4561 gattggtctg tagatagtgt agagcagaaa acaaaaggtg gagtcagaac aggatgaaag
     4621 tcggaggata aaaagtcagt tcaagtatgg aggattcagt ggttaccacc aggagcttgc
     4681 aatggggaca gcgaatacta acggctgcca ccgataaatt tcaaaaaaga gcatatacct
     4741 aatattcaac taaacagtgg catcttcaat ataatatatt aaagccccca tggagttacc
     4801 ctgaagggcc tcaatgtccg taattcctac ttatgtagga aatgttgtac agaacattta
     4861 ttataatcct attcaattat aataatcatg ccattattat atttaaacac tagagagtgt
     4921 cgttggtatt taatggggga aggtgagatg aaaaagatag ctgctatatc attaattagt
     4981 atttttctta tatctggttg tgctgtgcat aatgatgaaa caagtatcgg taaatttggt
     5041 cttgcatata aaagtaatat tcagcgtaaa ctcgataacc aatactacac cgaagccgaa
     5101 gcttctttag ctaggggaag aatatctggt gcagaaaata tagtaaaaaa tgatgcagcc
     5161 catttctgtg ttactcaggg caaaaaaatg cagatagttg acctgaagac agaaggtgca
     5221 ggattacatg gcgtcgctcg tctgacattc aaatgtggag agtgagaata ttttttggta
     5281 agcatcaaac atgcgcgttc tggttgtgct tagccggaac ctgtgcgagc acgatgccgt
     5341 tacgtgaaag gcatcgtgct atgaagggag attctatcga tgtggtcaat ggaagacggt
     5401 gaccagggat agggcttatg cataaaaaat aagcccgtgt aagggagatt acacaggcta
     5461 aggaggtggt tcctggtaca gctagcattt tatgggttat gtttttcagc gaaacggatg
     5521 ataaccttaa taaatgcagc tgtatgtgat cggtttctaa gaattttcca tccgggaaaa
     5581 ataatcgaaa ttaatcactt accgtgtggg ttacgcgtgg tttccccgga gaaattacgc
     5641 atcagcagcg cgtaatttag ctcaagatcc tgcgggaccg ggagccacac agtataacca
     5701 tcgcctggtg cgaccggcat cgcttcacct ttggcgtttt ccatgtgctc aagggtaaag
     5761 ttaatgttgc cttgcggcgt catcagctca aggctgtcgc caacggagaa tttatttttc
     5821 accgctaccg ctgcgaggtc ccccttgcgc tcaccggtaa actcaccaac aaactgctgg
     5881 cggtcagaaa ctgaataacc gtattcgtag ttctgataat cgtcgtgagt atgacgacgc
     5941 aggaaacctt cggtatagcc acgatgcgcc agaccttcca gagtttccag caggctggta
     6001 tcgaacggtt tgcccgcagc ggcatcatcg atagctttac gataaacctg tgcggtgcgt
     6061 gcgcaatagt agaaagattt agtacggcct tcgattttca gcgaatgcac gcccattttg
     6121 gtcaggcgtt ctacatgggc gatggcgcgc agatctttcg agttcatgat gtaagtgccg
     6181 tgctcatctt caaacgcggt catatactcg cccggacgct gggcttcttc gatcataaac
     6241 actttgtcgg ttggcgcgcc gatacctagc gtcggctcaa cattttgcac cggaatcggc
     6301 tcgtacttgt gtacgatgtt gccaacgtca tcttctttcc cttcctggac gttgtactcc
     6361 cagcggcagg cgttggtgca ggtaccctgg ttcggatcgc gcttgttgat atagccagag
     6421 agcaggcagc gaccggagta ggccatgcac agcgcaccgt gaacgaagat ctcaatctcc
     6481 atatccggca cctgattgcg gatctcttca atctcttcca gcgacagctc gcgagagagg
     6541 atcacgcggg tcaggcccat ttgctgccag aatttcaccg tcgcccagtt tacggcgtta
     6601 gcctgcaccg agaggtggat cggcatttca gggaagtgct cacgcaccag cataatcagc
     6661 cctggatcgg acataatcag cgcatccggc cccatttcca ccaccggttt caggtcacgg
     6721 ataaaggttt tcagcttggc gttgtgcggt gcaatgttga ccacgacata aaactttttc
     6781 cccagcgcgt gggcttcatt gatgccgagc tgaagatttt cgtggttgaa ttcgttgttg
     6841 cgcacacgca gggagtaacg cggctggccc gcataaacag catctgcgcc ataagcgaaa
     6901 gcgtaacgca tatttttcag cgttcccgcc ggggaaagga gttccggttt aaacataatt
     6961 ctctcgttct gatgacaggt cagctccgct tcacctgatg aagcggttag gggagtgccc
     7021 ccactttaag ggcgggcatt gtagcgctgt ggggggatct ggtaaagcgc cggacgccga
     7081 caccccacgc ccggcatcgt aattacgtat tgtctt
//
LOCUS       DAJTXQ010000179         7001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000179 DAJTXQ010000000
VERSION     DAJTXQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.179"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            365..1240
                     /locus_tag="OO515_003784"
     CDS             365..1240
                     /locus_tag="OO515_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6577606.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1248..2024
                     /locus_tag="OO515_003785"
     CDS             1248..2024
                     /locus_tag="OO515_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6577607.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2193..4454
                     /locus_tag="OO515_003786"
     CDS             2193..4454
                     /locus_tag="OO515_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6577608.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4523..5698
                     /gene="senB"
                     /locus_tag="OO515_003787"
     CDS             4523..5698
                     /gene="senB"
                     /locus_tag="OO515_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6577609.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6224..6685)
                     /locus_tag="OO515_003788"
                     /pseudo
     CDS             complement(6224..6685)
                     /locus_tag="OO515_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6723..>7001)
                     /locus_tag="OO515_003789"
     CDS             complement(6723..>7001)
                     /locus_tag="OO515_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6577610.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         1958 a         1642 c         1617 g         1784 t
ORIGIN      
        1 ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc gtattccccc
       61 cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc gcgggaatga
      121 aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa atcggtgttg
      181 caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat cggctggtaa
      241 acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat tcattctttg
      301 atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg tttcagatgg
      361 gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta gtctgggagc
      421 ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt tcctgactga
      481 cgggcagatt tacgatttgc acagtggcaa aattatttct agcagcgagc ttttggcgga
      541 tcttgctacc gctcgacatc ttattattgg tgaaaaacac gataatgccg agcatcatca
      601 gattgagctt tggcttattc aaaatttgct catccagcga ccacagggga gtgtgttgct
      661 tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt ggctgaaaga
      721 taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa aagggtggtc
      781 ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc cgctgctcaa
      841 tgccaacatt ggccgggagc agatgttggc actgtacaaa aaaaacgaat ttcctaaagg
      901 aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca tatctatgca
      961 tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctattcagc agcagcggga
     1021 tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga ttgcaggtgg
     1081 ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg ccaccggcac
     1141 ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag atcatgctga
     1201 ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg atgaatattc
     1261 tccacttctc acagtcggta aaatggagca gctggtttat ctgttctcta ctgcttcatg
     1321 gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct atgtctcctg
     1381 caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct ccactttcgc
     1441 ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa caaactgagg
     1501 acagacaaca acgtcctgtc actgaagaca gtgatgcaac cattgaaata actaggaaga
     1561 aaaagtcatc cgatggagaa aagaaaaaga cacgtccacc tcgtaagata aaggctcaaa
     1621 ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct ctggtcgaat
     1681 cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat tctgaggcat
     1741 cttgggaaag cagagtaaaa ggccacctaa accgttataa acgttaccct ggcgatgcca
     1801 gaaaacgtgc acgaaccggc acagccgtcg tgacttttac cgtaaacacg gaaggcacta
     1861 ttgtcagtag tttcttggaa atctcttcag gaaccctttc tcttgaccgc gaagctattg
     1921 ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg gagggaggat
     1981 tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat aattggccgt
     2041 tcaagctcat ttaactgaaa tttaattttc atttttatgc tgaacatttt atcaaacttt
     2101 tccactgtcg aaaaaatcaa aaagacagta gaacataatt aataaacaaa tagttatact
     2161 tatctttatt aatattatac ccaaggaaat ttatgaacgt tataaaactg gctatcggct
     2221 caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa aaaactaatt
     2281 ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg aagacgacca
     2341 gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg gacaaaaacc
     2401 tgcaatcact tgacgcaaca gtgcgtagta tgcctggtac ttatactcaa atagatcctg
     2461 gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt gtaaacacta
     2521 tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca acgacgcatg
     2581 gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta gcagttgatg
     2641 ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt agtgcaaata
     2701 tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc cttcgttcac
     2761 gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt ggtggaaaaa
     2821 gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc ggcagttctg
     2881 tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt tatgataaat
     2941 atatgaagca gaaccccaaa tcccaactgt ataaaatgga tatcagacca gacgaattta
     3001 acagcttcga actttccgct cgaacctatg aaaataaatt tacacgtcgt gatataacca
     3061 gtgacgacta ttacattaaa tatcattaca cccctttttc tgaattaatt gactttaacg
     3121 taacggccag taccagtcgc ggtaatcaaa agtatcgtga tggctcgctg tatactttct
     3181 acaaaacctc agcgcaaaat cgttctgacg cgctggatat caacaatacc agccggttca
     3241 ctgtcgcgga caatgacctg gagtttatgc tgggcagcaa actgatgcgt acccgctatg
     3301 accggaccat tcactcagcg gcgggcgacc cgaaagcgaa tcaggaatcg atcgagaaca
     3361 atccgttcgc accctccggc cagcaggata tttcagcgct gtataccggg ctgaaggtta
     3421 cgcgcggcat ctgggaggca gatttcaatc tcaactacac acgtaacagg atcacagggt
     3481 acaagcccgc ctgcgattca cgcgttatct gcgtgccaca gggtagctac gatattgacg
     3541 ataaagaggg tggcttcaac ccttcagttc agctttctgc tcaggtaaca ccatggcttc
     3601 agccgttcat tggctacagc aaatccatgc gcgccccgaa catccaggag atgttcttct
     3661 ctaattcagg aggcgcatcc atgaacccat tcctgaagcc tgaacgtgca gaaacctggc
     3721 aggcgggttt taacattgat accagagatt tactggtcga acaggatgcc ctgcgcttta
     3781 aggctctggc gtaccgcagc aggatccaga actacatcta cagcgagtct tatctggttt
     3841 gttctggagg tcgtaaatgc agtatgcctg aggtgattgg caatgactgg gagggcatta
     3901 gcgatgaata cagcgacaat atgtacatct acgttaactc ggcaagcgac gttatcgcaa
     3961 agggcttcga actggagatg gattatgatg caggttttgc ttttggccga ctctctttca
     4021 gccagcagca aacagaccag ccaacctcca tcgccagcac ctactttggc gcaggggata
     4081 tgaccgaact gcccagaaaa tacatgacgc tggatactgg tgttcgcttc ttcgataacg
     4141 cgttgaccct gggcactatc ataaaataca caggcaaggc tcgtcgcctg tcgcctgatt
     4201 ttgagcagga cgaacatacc ggcgcaataa tcaaacagga tttgccgcag atcccaacga
     4261 ttatcgatct ctatggtact tacgagtaca accgcaacct aacactgaaa ctttcggtac
     4321 aaaacctgat gaacagagat tattcggagg cgctgaataa gctcaacatg atgccaggtc
     4381 ttggtgacga gacccaccca gccaattccg cgcgtggcag aacatggata tttggcgggg
     4441 acattcgttt ctgatgacaa tttccgctag gttcagacaa tacgttttct ctcttatgtc
     4501 aattttattg caggaacgaa aaatgaatat tttcacttta tccaaagcac cgctatacct
     4561 gttaatttca ctatttttac ccacgatggc catggctatc gatccacctg aacgcgaact
     4621 ttcgcgattt gccctgaaaa cgaattacct tcagtcccct gatgaaggcg tctatgaact
     4681 ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc accgatcgtg taaatcgtga
     4741 agccaataaa ggctatctgt attcgtttaa ttcagattcg ctgaaagtcg aaaataaata
     4801 cacgatgcca taccgggcat tttcgctggc gataaatcag gataaacatc agctctatat
     4861 cggacacacc cagtcagcgt ccctgcgtat cagtatgttt gacaccccaa ccggcaaact
     4921 ggtaagaacc agcgacaggt taagttttaa agcggcaaac gctgcagatt cgcgttttga
     4981 gcattttcgc catatggttt acagccagga ttccgatacc ctgtttgtga gttatagcaa
     5041 tatgctgaaa acggccgagg gcatgaagcc tctgcataag ctgttaatgc tcgacgggac
     5101 gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa ggtacagcgt atggtctgac
     5161 gatggatgaa aaaacacaga aaatctacgt tggcggaaga gattacatca acgaaattga
     5221 tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa gatccgagac cacaaatcac
     5281 aagtgtgcag aatctggcgg tggactccgc ttctgaccgt gcctttgtgg tggtattcga
     5341 ccatgacgat cgttccggta caaaagatgg actctatatt tttgacttac gcgacggtaa
     5401 acagcttggc tatgtgcaca caggagccgg agctaacgcg gtgaaataca atccgaaata
     5461 taacgaactg tatgtcacca acttcactag cggcaccatc agcgtagtgg atgccaccaa
     5521 atacagcatc acccgtgaat ttaacatgcc ggtctaccca aaccagatgg tgttgtcgga
     5581 cgatatggat accctttaca ttggcatcaa agaaggcttt aaccgcgatt gggatcctga
     5641 tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt gatttgaaaa agtcgtgatc
     5701 ggggggaaaa aacatgggat aaaaactccg ttattccact aaactggccg gactaggaaa
     5761 agcaaacatc tctgtcctgc cggtacgcct gttacgccgc tccatgaggc tgaggaatct
     5821 ccagaaaacg agacaggcac agcattgtac gatcaactga tttgtgccaa taattaaatg
     5881 aaatcacagc tatgcctgtt catggggttc taggccggaa ccccagattt ttccgattgt
     5941 ttgttttcta actgattatt cagtagtttt agagggctga cgctcagtgg aacgaaaact
     6001 cacgttaaga ggttttgact atgataaaag tcaaagtagg ggtgagtttg gatatggaaa
     6061 ttattgtact ataaggagag catgaaaacc cctggggcag tcactgaatg agggaccagc
     6121 agcggtaaaa taaccagcat aatttctgca tggtcgattt ttataccgta acaggctgtt
     6181 ttttatactt ggggtcagtt tggatatcga aaattattgt acgttaaggt tgttttttga
     6241 ccttgtcgat cttaactgat ctcaaacgac gaaacttacc tgaagtcggt atccggctgt
     6301 ttttccagat ataatcgcct gtcagattga tatgttccca acctaatggt gataaatgcg
     6361 ataatagttg agcatttatt ttcatgccct tacgcttcag agcatcaacc gctctttcca
     6421 tgtaaactgt gttccataag gaaatagccg ccgtcaataa agtcaatcca ctggcgcgat
     6481 aactttgatt ttccggtttc cgatctctaa tctcccccaa tcgatgcatg aatacagcac
     6541 gggccagagc attacgggct tctcctttgt tcagcccggc ctggacacgt cgccgcaatg
     6601 ccggatcccg aaaccagtcc agcataaaca gtgttcgttc tatacggcca atctctctca
     6661 gagctttagc caacccattt tgtttgtaag cgtgcagcaa gaaccgtatt gacgggaatg
     6721 tgttattcag tcggcagtgc tacgcgccag gggagcagtt cgccgacccg gtttatcggc
     6781 cagtcggcta tgacgtcaag gacatagcgg aggtagcttt ctggctccac tccgttcagt
     6841 ttgcacgtcc cgatcaggct gtacagcagc gctccccgct ctcctccatg atccgaaccg
     6901 aagaacaggt agtttttgcg gcccagactg accatccgca acgcattttc agcgatgtta
     6961 ttgtccgcct cagcccagcc atcatctgca tagtacgtca g
//
LOCUS       DAJTXQ010000180         6762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000180 DAJTXQ010000000
VERSION     DAJTXQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.180"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..204)
                     /locus_tag="OO515_003790"
     CDS             complement(61..204)
                     /locus_tag="OO515_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCR6577611.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(302..553)
                     /locus_tag="OO515_003791"
     CDS             complement(302..553)
                     /locus_tag="OO515_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HCR6577612.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(621..2291)
                     /locus_tag="OO515_003792"
     CDS             complement(621..2291)
                     /locus_tag="OO515_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HCR6577613.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPDLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2524..4743)
                     /locus_tag="OO515_003793"
     CDS             complement(2524..4743)
                     /locus_tag="OO515_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6577614.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4922..5188
                     /locus_tag="OO515_003794"
     CDS             4922..5188
                     /locus_tag="OO515_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6577615.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARIRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5402..6328)
                     /locus_tag="OO515_003795"
     CDS             complement(5402..6328)
                     /locus_tag="OO515_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6577616.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1597 a         1889 c         1547 g         1729 t
ORIGIN      
        1 aaaaaaaagc gcgtcagaat cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg
       61 ttactgcgtt gctaatccgg tcagttcagc tgaggatgac ggggatacag tcccgccctg
      121 aacatcgcgg atatagtttg cctgacatgc agcaagaata atggctgcaa gaagcaataa
      181 aataatccct gttatttttt tcatactgtt ccttgtatat atggactcag gcattcatcc
      241 ataaaaacct gaaatccaaa ggttgaaatc atccatgaaa cataaaattc tataacacta
      301 tttacatctt taacgtgata atgaaagcag tgtatccatt ctcaatatca tctttaatat
      361 tttcaatagc caatctatta ttttcaggga tttttgattt gtattcctcc ggcatatcat
      421 atgtccatga tgctgaacta tctatcctga atccaggatt cagccaatgc ccaccatcaa
      481 tgcccaaaat ccataagcct tttttcgctg cttcattaca aacttctatt gcagcttttg
      541 gtgataactt catagaagca tttcctttta attcaaaaaa ggcatcacca ctatcatata
      601 acaccgtatg ttcaaataat ttattggtca tttttatttc cccattgaat tctcgaatcg
      661 tccacccaac ccggatctgt cttatcacta atctgagtaa cctcaccagt acgatcattg
      721 acaacaacat atttaccagg agaaccatac actgttgctg gatcatttcg acctaaatag
      781 tctggaggtg ttttcttcgg actacgctta tcaaaagatg taccagttgc accgttagat
      841 acggtatttt tgatatcatc ctctgtccat cctctttctg gcatttgtcc cctaattttc
      901 tgatctattt ttagatctgg tatctttcct ttattttttg ctaactcatc atttagtttg
      961 ccttcagcat ctcttttatt atcctctttc ttcttcctgc tttccatcgc agtacttaat
     1021 gcagcatcag catctgcctt ctcttttgca gcagcatcaa atgcagcctg cttattattt
     1081 acatccgtct gcgcccgctg ggctttaagc ccggccattt gccacattct gtgaccgcca
     1141 gccattgggt catgtgcaaa tcgttcaaat tgttttattt cagctattgc atcagcaaga
     1201 gttttattcg ctgcatcaag ttcgctttta cgcgaattat aaacctgaac agctttagcc
     1261 tgtcgctcct gatttctggc aacatcttca tttgcctgat tcagctctgc acgcgcgcgt
     1321 tcataatttc gctcagccgc ttcaaccgga tgcgtagcat cccattcctg ctgacggcga
     1381 ttttcttcat cctgacgttg ttttacctgg tcagggctaa gaacatcact cactgaaaca
     1441 tatacggcat tatgaccgct gtccttcggg aatcgaataa ctgcatccct ggtattaccc
     1501 ccctgagtaa atcctgccgg gcgaacatcc ttatcagtat tatttgtaac acctgggctt
     1561 aatgtctgta ctgctggcgt actgttatta actgaaatat tcagaacagg tgcacctgga
     1621 attgatgccg taaaaacacc tggacgttcg gtaggttttg catcaaccac cggaacactc
     1681 atcggaacac ctgaaacaac cgaaatattc tgtcgttcgt cttttacatc atcaacaaca
     1741 cgaacattta cgtttactgt tgccttatcg agaggtaatg aactgacagg tgattcagta
     1801 atatcatctg cgggtaatga cgtcacaatc tttgacatca tattggggtc atctttcgct
     1861 atttgtgatg gcaatacacc atataaagcc accccccaaa gaccaaattt aaacggtcct
     1921 ttcagggcag ccataatatc agcaatagct gccgataatg ctcccgctga aatactgacc
     1981 gccagaccgc cagcacctgg agtggaaagt gccggaaaac caaatgccac tggcgcagct
     2041 actgctgaca gattaccgcc tgttcccgag ccaccaccgg aattaccatt ccccccgcca
     2101 ttaccatgac cggaaccacc tccccagtga atgccgctac cggaaccacc accccacggg
     2161 ttattttccg aactccaccc ggaaccatca gaagcaccac cacctacacc aagcccggtc
     2221 gggccaccat taatgttacc acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg
     2281 ccaccgctca taaaaattcc ctcttttaag cgttaaaaca attaattaca cgattaatac
     2341 tgtacataaa accactggtt ttatgtacag atcaaaaagt acccgtcatc gttttttctg
     2401 tcaagctcag gtggaaaatt ttttagaaat caggagggat aacacttaga aaacgcagag
     2461 cgggccacaa aaaattttgt ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg
     2521 acatcacata ctcatgcccc ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc
     2581 gtcccgcttc cactcatgcc aggtctgatt aaaaccgagt tgacggaaga gataatccgg
     2641 catctcttcc ggtttgtgga cagcagccaa aagcctgtca tgatggcctt tgagccggaa
     2701 ctgcatcagg tgcggttccg gtgggtacgg agtatcctca tcatggttta tagcttctcc
     2761 gtaacctgcc aggcataccc cgttctccgt gatggtgctg actatcagat gactgtaatc
     2821 cggcgacgga agcacaagaa cggggcaaaa ccctccttcc cggcgaacaa acccgacaag
     2881 catttcctcc ggccctttgc ataacaacag attcagcgtc ctgctgtttc cctctctcga
     2941 aaatacaaca ggcatgagtt gttccggtac agcatcagcg gtgatatcca cccggtgcgc
     3001 tgacggcagc ggagaggcta cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca
     3061 gtgatgcggc agcgccggaa aaccgtaaga gggccacccc tggcgcatct gttccagtac
     3121 atgccggacc gtcgcaccgg aatacattct cacacgctca ggccgcgcca tcgtctcatg
     3181 agattctggc attccctcac gtatatccga gaccatcaca gccccgcctg gcgacaggta
     3241 aacattcacc gggtcatgaa cactctgggg gtaatgcgcc agctcatcac cccataccgt
     3301 gaattctccg gctctgttac tcagcatcat gtagcgggtt atcctgcccg ttcccggctc
     3361 tctggcacgc cccagctccg taataacccc gactaatgcc gtttcacggc tcagacggtg
     3421 gttatcatga cgtttcagcc gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc
     3481 ggcttcctcc ctgcgccgct ccgcttcccg tgctgacagt ttttctttca gctctgccat
     3541 tcccggcgca atccacgtat tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc
     3601 ccgttctttc acggccctgt aggtgcggcg aagagattca atctgtttcc cgagtttcca
     3661 gaaacggagt tgtcttttgc ggtattccgg ttcagcccat gtcatcaggg ggatctctga
     3721 cagaagggaa gcgacagggc gtgctttttt gagtccggtc atctcctttt cgagaatggc
     3781 ctgctctttc agcagcgcat tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc
     3841 caggaactcc ggggtcatct cccacgcgtt gccgcattcc tgcggcaacg tggcaatgaa
     3901 ttcccgcagg gtatccgctg aggcattcgc agcgggaatg gtcaggttaa gggcttccgc
     3961 tgcacgacgt gcggcatcgc tgagttcccg tttccgcacg ttctcctgta cctggatgac
     4021 tgccagcctt gtgcgggaaa cctgtatttc caggttaagg ctgttaatca gacggttctg
     4081 ctcaccgcgt tctgtttcca ttccccgttt ttccatcgca caggcagcct ttccgaggtg
     4141 aaggccgggc gctttctcca gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg
     4201 gtagccggcg ttcgcaagcg ctctgttcgc atggtcagcc catgatgcgc gccacgtctc
     4261 ggcgagtgcc cggtcgttcc agtccctgac ctttcctgca aatcccgttt ccccgacagc
     4321 tctcgtggtc agcatgatgt gcgcatgggg attggtccgg tccatgtgat ggaacgccac
     4381 atcggcaatc atgccccgac tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg
     4441 tgcggcctcg cgggaaatct cacggggaat ggcgagttca atctcccttg ccagctggga
     4501 gttccggcgc tgttcggctt tctcgaccgc attccagaga acggaacggt caccacacca
     4561 ggacggtgca ttcgcagggg tgagaatgac cgcatcatca acgccgcgtt tacgggtgta
     4621 atcgtgggtt aatccggtac ggtcatcggg tatgacagag ccggaacggt aggcagcaga
     4681 agcaacggca ctgtagccgt tttttcgcga aatgattttc atgctgagat gataaatcgc
     4741 catttctgcc gtaatgcctt tttgccgttt tcttttagcg tcgcagaccg cacacaatcg
     4801 aaacgaagtg gagattgtat aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc
     4861 agttttgcaa ctaccagtga cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa
     4921 aatgagcaga acactcgaac agaagattgc cgaagctgag gccagattac agcgcctgaa
     4981 ggcgaagagc cggagcctgg acacggcgca aaaagtcatt gtgggggctg ccatgctggc
     5041 gaggatacgg aggccggaag aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa
     5101 ggaagtcaca agacaggccg atgtgaaccg gatacaaccg ctgattaacg agctggagaa
     5161 gctacccaaa ccacctgcga aaccgtagaa cggagaaacc ccggcgcgta agtgccacat
     5221 gcaccaccga cacctttgcg cttcctcgct cattcgctcg ctacgctcgg tcatgagact
     5281 gcggcgagcg ctacaacctc aatcatgcgg aaagccgcac cattgcaggg aggaaacgaa
     5341 aaagccacca aacgccaaca gctcaaaaaa gcgctgttat ctgatatagg ttttatttgg
     5401 tctactctac aagacctcgt tttttcagtt ggtaatacca ggcgcggcta attccaagag
     5461 tctcccacgg tttcaacgtt cgcgcagatg tggcaacagc accacgctta gacttagcac
     5521 ctccaatttt cccgcccttg cgaccacgtg cagcctgaat ttctggcgtg tgcgtatcag
     5581 ccacatactg tgcgaaagtt tccggcgtga agttcctgtg cgtgtatttc gcaatgctct
     5641 tgccaatagc ccgacattcc gccggagaaa gcggaacggg aagcgatacg ttgtacattt
     5701 cgacacgctg gatcacggca tcaagccatt gtgagaatac aggccagccc tgacgaatcg
     5761 ccctgtaagc ccatttgcgc gtcatttcga acaggtggca gtttcgcccc atcccgtaat
     5821 gtttatcgat gctacggcgc gctgaggcgc tcaaatcgag ataatcagcc agttcatcga
     5881 gggtataggc ttcctcgcgc cactccatca ccagccattc caggtggaac ggatttttgc
     5941 aaatcagtcc gctgtagttc acatctgcac caagtttctc acacagcgca cgctctatcg
     6001 cggcagcgta cttcagtgcc ttaaccgacg catcaggcgc ggttctcacg gcgatgttga
     6061 gtgcatagag caggtgagca tgaccgttca cgggattttt tacggtgatg ttcggcgcgg
     6121 gggcgttccg gtcgctccag tcaatcgcag ccccctcgcg gtcaacgtca aaaaccagcc
     6181 agaactgcgc atgaggctgg ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc
     6241 cggagatgcg aagaccaaaa gcgagatcat ccgtgtgata gggacggtgg ggtaaatttt
     6301 cttcgaagta ttgaagcgcg gcgctcatgt gttacgattc cttccaggtt atcgaccgac
     6361 ctcgcccctg tattctcgtg acaatcttgg cggagagaca cgaatttcag gggcgtttgt
     6421 ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga
     6481 tccatttacc ataactcccg ttgtacgtac cccttaaaac gccctcaatc gctttctgac
     6541 gcaaaaaacg caaaaagaca aggaaacaca gggtaaacat aaaaaaatgc gtcagaatcg
     6601 cttttagcgc gttgcataac tatgcacgaa agcgatgcgc gatcggtttc gtgcgttctg
     6661 aacaccgaac ggctcaaaac gacaagtttt ctccccctgg atggaaagtt ctgcacgagg
     6721 attttagatc ccatctccca catggaattt ttgtgacact tc
//
LOCUS       DAJTXQ010000181         6648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000181 DAJTXQ010000000
VERSION     DAJTXQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.181"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..738)
                     /locus_tag="OO515_003796"
     CDS             complement(<1..738)
                     /locus_tag="OO515_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6577617.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(879..2912)
                     /gene="metG"
                     /locus_tag="OO515_003797"
     CDS             complement(879..2912)
                     /gene="metG"
                     /locus_tag="OO515_003797"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6577618.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3044..4153
                     /gene="apbC"
                     /locus_tag="OO515_003798"
     CDS             3044..4153
                     /gene="apbC"
                     /locus_tag="OO515_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6577619.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4398..4679
                     /locus_tag="OO515_003799"
     CDS             4398..4679
                     /locus_tag="OO515_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6577620.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4922..5464
                     /locus_tag="OO515_003800"
     CDS             4922..5464
                     /locus_tag="OO515_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6577621.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5544..6218
                     /locus_tag="OO515_003801"
     CDS             5544..6218
                     /locus_tag="OO515_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6577622.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6234..>6648
                     /locus_tag="OO515_003802"
     CDS             6234..>6648
                     /locus_tag="OO515_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6577623.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1758 a         1507 c         1592 g         1791 t
ORIGIN      
        1 aatatcaatc tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg
       61 gacgatctga tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc
      121 cgtgagtaca gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact
      181 gatcgcctgg gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc
      241 tgttttatta tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc
      301 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
      361 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      421 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      481 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      541 ctccacatac cccttcttca ccttctccgc aatcagctta agctccgctt ttgccgctgc
      601 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      661 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      721 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      781 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      841 aagaaaaggc gctgtcgatg cagcgccttg aagagggatt atttcacctg atggcctggt
      901 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      961 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
     1021 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
     1081 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
     1141 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
     1201 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
     1261 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1321 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1381 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1441 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1501 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1561 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1621 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1681 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1741 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1801 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1861 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1921 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1981 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     2041 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     2101 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     2161 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     2221 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     2281 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2341 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2401 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2461 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2521 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2581 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2641 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2701 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2761 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2821 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2881 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2941 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     3001 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     3061 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     3121 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     3181 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     3241 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3301 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3361 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3421 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3481 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3541 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3601 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3661 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3721 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3781 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3841 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3901 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3961 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     4021 cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc
     4081 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     4141 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     4201 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     4261 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4321 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4381 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4441 gattggcttc tcccgcattc tcttcagaaa ctgccacatt agcgattaat ggcaggatca
     4501 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4561 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4621 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4681 ttggcaacct gatttttatt gatatcaaca ataccattta cataaatcta cattaattga
     4741 tagatgcgat aaatagttaa tactcttaaa tatttgttgt atttatattg caatggttaa
     4801 tttgtttcaa ataaataaaa caatgtgttt acattgcggt aacttttttg atgtgagaaa
     4861 tttgtttcca agagatacag actcttaaca accaaaaaaa aattttatta aggaacatat
     4921 tatgaaacgt tcaattattg ctgccgctgt cttttcttct ttttttatga gcgctggagt
     4981 atttgctgca gacgttgata ccggaacatt aactattaag gggaatattg cagaatctcc
     5041 gtgtaaattc gaagcgggtg gtgattcagt aagtattaat atgccgactg taccaaccac
     5101 tgtctttgaa ggtaaagcta aatattctac ctatgatgat gcagtcggtg taaccagcag
     5161 catgttaaaa attagctgcc cgaaagaagt tgctggtgta aaactctcgt tgattaccaa
     5221 cgataaaata accggtaacg ataaggcgat agccagtagc aacgataccg tgggttacta
     5281 tctctattta ggtgataaca gcgatgtcct ggatgtttct gcacctttta acattgagag
     5341 ttataaaaca gcggaaggtc aatatgctat tccgtttaaa gcaaaatacc tgaaactgac
     5401 agataactca gtgcaatcag gtgatgtgtt atcttctctg gttatgcgtg tggcgcagga
     5461 ttaataaata aaattgtaag tttctccagg taataaacat ggccgctatc ccatggcggc
     5521 cttttaattt aagaggcatt aaaatgaaag gattattatc tttactcatt ttttctatgg
     5581 tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac ccggcagaaa
     5641 ataaagaagt gatggtgcag ttgatgaacc agggaaaccg ttcttcgctg ctgcaggcgt
     5701 ggattgatga tggcgatacg tcattaccac cagaaaaaat tcaggttcct ttcatgttaa
     5761 cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc
     5821 cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg gatattccac
     5881 caaatagtcc agagcaagaa ggtaagaatg cactgaagtt tgcgatgcaa aacagaatta
     5941 agttgtttta ccgaccagcg ggtattgctc cggtaaataa agcgacattt aaaaaattgc
     6001 tggtaaatcg cagtggcaat ggtttggtga taaaaaatga ctcagctaat tgggtgacga
     6061 tttcggatgt caaagctaat aatgtcaaag tcaattatga aactattatg attgccccct
     6121 tagaaagtca gagtgttaat gtcaaaagta ataatgcaaa taactggcat ctgaccatta
     6181 tcgatgacca tggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga
     6241 gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag
     6301 ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa
     6361 atattcgtct tgatgataat caacccttac cggggcagta tgacatcgat atttatgtca
     6421 ataagcaatg gcgcgggaaa tatgagatta ttgttaaaga caacccgcaa gaaacatgtt
     6481 tatcaagaga agttatcaag cggttaggca ttaatagcga taacttcgcc agcggtaagc
     6541 aatgtttaac atttgagcaa cttgttcagg gtgggagcta tacctgggat atcggggttt
     6601 ttcgtctcga tttcagtgtc ccgcaggcct gggtggaaga actggaaa
//
LOCUS       DAJTXQ010000182         6634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000182 DAJTXQ010000000
VERSION     DAJTXQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.182"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..312
                     /locus_tag="OO515_003803"
     CDS             <1..312
                     /locus_tag="OO515_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577624.1"
                     /translation="PVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQVRCKICGA
                     VVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARIRRATKSRT
                     E"
     gene            405..623
                     /locus_tag="OO515_003804"
     CDS             405..623
                     /locus_tag="OO515_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6577625.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKEN"
     gene            778..1011
                     /locus_tag="OO515_003805"
     CDS             778..1011
                     /locus_tag="OO515_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6577626.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(1044..1682)
                     /gene="ompW"
                     /locus_tag="OO515_003806"
     CDS             complement(1044..1682)
                     /gene="ompW"
                     /locus_tag="OO515_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6577627.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1922..2011
                     /locus_tag="OO515_003807"
                     /pseudo
     CDS             1922..2011
                     /locus_tag="OO515_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2039..2782
                     /locus_tag="OO515_003808"
     CDS             2039..2782
                     /locus_tag="OO515_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6577628.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2812..3351
                     /locus_tag="OO515_003809"
     CDS             2812..3351
                     /locus_tag="OO515_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6577629.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3456..3854
                     /gene="yciA"
                     /locus_tag="OO515_003810"
     CDS             3456..3854
                     /gene="yciA"
                     /locus_tag="OO515_003810"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6577630.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3894..4613)
                     /gene="tonB"
                     /locus_tag="OO515_003811"
     CDS             complement(3894..4613)
                     /gene="tonB"
                     /locus_tag="OO515_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6577631.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4704..6224
                     /locus_tag="OO515_003812"
     CDS             4704..6224
                     /locus_tag="OO515_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577632.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6257..>6634)
                     /locus_tag="OO515_003813"
     CDS             complement(6257..>6634)
                     /locus_tag="OO515_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577633.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1741 a         1524 c         1591 g         1778 t
ORIGIN      
        1 ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc atcggaaaga
       61 ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag agcgtcaggt ccgctgtaaa
      121 atctgcggtg cggttgtgga gccgtttgac tggatgctct ctgtggcaaa aagagaaacc
      181 agactggcag atgatgtaag gctattgcgc caggaggaac aggaaaggcg gagaaatata
      241 gaaaagctga tacagattga gcgtaacgcg aaagcgcgga tacgcagggc gacaaaatcc
      301 agaactgaat aattaaattt agcactgtta aaaatttaat ccttaaccgg agggatttct
      361 gcaccctcaa atcatcagga gaccgcccga aagggcggta atgaatggtt acattattta
      421 gaaaaaaata tccgcgaaag agtagaacaa cagaattcct gtttctcatt ctgtttatcg
      481 tgttgatgac accgatatcc ccgctaattt ttgtctggat aatcggaaaa ataattgagc
      541 cagttattga attgtataac gacgtggtat gggcgtcatt caacacactg cacaataaaa
      601 ttaatccgta taaggaaaac tgatatggca actttgacaa aaaaagaacg ggcatggttg
      661 aacgaattac aggaagttct tgatcgctgt cctgtggtgt catcctgccg cgattgcgtt
      721 cttagtccgt ggctattgca ccaccatcac gcgaaaggga cagctaagcg cggcgggatg
      781 gttaagccag caacattaac cgttgcattt aaaaaagccc gggattctgt ggattacaac
      841 tggcgtgcta atggcacccc accctctttc catgagcaga gatctttatc agagcgattg
      901 ttcagagagc agggggttga taccaaaatt ttgctaggcc attcgaatca aaaaatgacc
      961 gatatttaca acgatgtacg cggtaaggag tggaaaaaac tggtcatttg ataaccagtt
     1021 ttgcagaggt tttgcagaga aaattaaaag cggtacccgg cagagaacat aaacacccac
     1081 ggatccagtc gtaccgagtc ttttacggta gtaacaccag atttgtactt agccgtggta
     1141 tcgatatcca tgtaccacac tgacatgtta accaaccagt cacggttaat cagataatca
     1201 acccccacct gcccggcagc tccccagtaa tctttcagac tgagatcgga aagccccgcc
     1261 tctttgccat gatcgttaaa tccattatca aagaaggtgg tgtagttaat acctgcccca
     1321 acataaggac ggaatttgct gccggcatca ccaaaatacc actgcgccat cagtgttggc
     1381 ggcagatgat gaacggttgc aatatcgccg gtcgcccggg tgccgatttt atggcggaac
     1441 ggcgtcgctg ccagtaattc cacaccaatg ttgtcggtcg ccatataagt aaacgtcagg
     1501 cccagttgcg tgttattggt cacgctgaat ccacccagac ttcctaacgt accaccagca
     1561 ccttctgttg gacgtacggt tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc
     1621 gcaaaggcac tgccagagag aagagttgtt actgccaaag ccgccactgt taactttttc
     1681 atatccgctc cgtcgttatg gttataaaaa cgtgacgaat atacctacat tggagtaaca
     1741 aatgatccta catagatcac attacatatg ataattcacc ctttattgat ctggattaat
     1801 ttagaagttg cattgagaat cctggttaac gtgatttaaa tcaattttta gatattttcg
     1861 ctacgcaaat attaactcgt tcgccacttg ccgcggtttg cccacgcctg aaggccagca
     1921 attacctccg ctttacaaag atatgctttt ccatacaaga cctgatgctg ccacccgagg
     1981 cttaaccagg gtacaattgc ccgctaatta acacctgcat aaaactcaag gagagtgcat
     2041 gtctatcacg gcgcagtccg tataccgtga caccggaaat ttcttccgta atcaatttat
     2101 gaccattctg ttggtatcgt tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt
     2161 ctcacccagt gatgcacagc ttgcgcagct caatgacggc gtgcccgtta gcggcagtag
     2221 tgggttgttc gacctggttc agaatatgtc accggaacaa caacaaattt tgctgcaggc
     2281 ttcagcggcg tccacttttt caggattaat cggtaacgcc attctcgccg gaggcgtaat
     2341 attaattatc cagctggtgt ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc
     2401 cagtgcgccg atattgccca agttatttat tctgattttt ctgactaccc ttttagtaca
     2461 gattggcatc atgctggtgg tcgttccggg aattatcatg gccattttac tggccctggc
     2521 accggtgatg ctggtacagg acaaaatggg catttttgcc tcgatgcgta gcagtatgcg
     2581 gctgacttgg gcgaatatgc gtctggtggc acccgcagta ctgagctggt tgctggcaaa
     2641 aacactgttg ctgctttttg cctcttcttt tgccgcatta accccggaaa ttggtgccgt
     2701 actggcgaac accttgagca acctgatttc agccatattg ctcatctatc tgttccgcct
     2761 gtatatgttg attcgccaat aaccttaagc atttgattac ggaatcgtaa aatgaagcag
     2821 tttcttgatt ttttaccgct ggttgtcttt ttcgcgtttt acaagattta tgacatctat
     2881 gcggctactg cggcgctgat cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc
     2941 tttcgtaagg ttgagaagat ggccctgatc acttttgttc tggtggtcgt cttcggtggc
     3001 ttgacgctgt tcttccacaa tgatgagttt attaaatgga aggttacagt catttatgct
     3061 ctgtttgcgg gagccctgtt agtcagccaa tgggtgatga aaaagccgct aattcagcgg
     3121 atgctgggta aagaactcac gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg
     3181 gctgttttct ttatcctttg cggtctggca aacatctaca tcgcattctg gctgccgcaa
     3241 aatatttggg tcaactttaa agtctttggc ctgaccgccc ttaccttaat cttcacattg
     3301 ttaagcggta tctatatcta ccgccacatg ccgcaggaag ataaatccta accagactgc
     3361 cagtccttcg gggctggcgc tgtatctcct ctgttctcct caggaaaatc atagtagcat
     3421 cgcgcctgtg attttccttt taagtcggtt ttaccatgtc tacaacacat aacgtccctc
     3481 agggcgatct tgttttacgt actttagcca tgcccgccga taccaatgcc aatggtgaca
     3541 tctttggtgg ttggttaatg tcacaaatgg atattggcgg cgctattctg gcaaaagaaa
     3601 ttgcccacgg tcgcgtagtg actgtgcggg ttgaaggaat gactttctta cggccggttg
     3661 cggtcggcga tgtggtgtgc tgctatgcac gctgtgtcca gaaagggacg acatcggtca
     3721 gcattaatat tgaagtgtgg gtgaaaaaag tagcgtctga accaatcggg caacgctata
     3781 aagcgacaga agcattattt aagtatgtcg cggttgatcc tgaaggaaaa cctcgcgcct
     3841 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tacttactga
     3901 atttcggtgg tgccgttaat tttaaacagg atattcacca caatcccact gcctggctta
     3961 cccggctcat aacgccatct gcgcatcgca tttttcacct cacgctcaaa catgttcgca
     4021 ggcttggctg agaggatttg tacgttatcc acgcgaccat ccggcgtgac gtcaaattta
     4081 actttaacct gcccttcaat gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta
     4141 cggcttaatg cgcgtggtcc tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt
     4201 gccgtacttg atgtggggcg cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac
     4261 tctacgggtt tgacatcgcg ttttggctgc tcctgtacct ttttcaccgg cttcggtttt
     4321 ggcttaggtt tcggcttcgg cttttcaatg accaccggtg cttcttttgg cggttcgggg
     4381 atcggctcag gttccggttc tggctctacc accggctccg gtggcggctg aacggcttgt
     4441 ggcggttcga gatcagcagg cgcaaccatc gtgacagaaa ttggctgcgc aggcgcaggt
     4501 agttcaataa cctgatgtac cgaggtatag agcagacccg ccacaacagc accatgaatg
     4561 cagaccgaaa gtaacgtcgg ccaggggaag cggcgaggta aatcaagggt cattgtattg
     4621 tagctattcg gcattgtagc attagccata acgtttataa gttcatcttt aaatgataat
     4681 caattgcatg ggagtttatc accgtgagaa ccgtaataac atacttgaga ttctcatcag
     4741 ccatacaagg cgctgaaggt gcagattcaa ccagacgaca aaacgacctg ttcaagcagt
     4801 ggctgaagaa gaatggcgat gctcaaatag tagcgtcttt cagtgatgaa gggttgagtg
     4861 gttacaaagg taaacacctt actggtcagt ttggtgacat gttagcccgt attgaggctg
     4921 gggagtttcc agaaggtaca attctgctag ttgagagtat cgaccgtata ggccgattag
     4981 agcatcttga aacagaagcc ttgatgaacc gcatcttagg caacgggata gagattcaca
     5041 cattgcaaga tggccttatc tacacaaagg atgctttagc cgatgacctt ggcataagca
     5101 tcatccagcg tgtcaaagcc tacatagcgc atcagaaatc taagcagaag tctttccgtg
     5161 tttctcagaa atgggggcaa cgtgcaaaac ttgcccttgc tggtgaacaa cgtcttacaa
     5221 agatggttcc tggatggatt gaccctgaga cttttaaact caatgaacac gctgagactg
     5281 tgagattgat attcaaactg ctgctagatg gtgaaagtct gcataacatt gcacgtcacc
     5341 ttcaaagcaa cggtataaag tcgtttagtc gccgtaaaga tgctaatggg ttctctgttc
     5401 actctgtacg caccattcta aggtcagaga caacaatagg cacgttacca gcatcacaac
     5461 gtaatgaccg ccccgctata ccgaactact acgaaggtgt tgtagatata ccaacgttca
     5521 ataaagctca agagattctc gacaagaatc gtaaaggccg tacacctgca agtgacaacc
     5581 cactaacgat taacatcttc aaaggtctgt ttaggtgtca gtgtggggct agtgtccatc
     5641 ctaccggaac aaagaataag tatgctgggg tctacaggtg caataaccac ttagacggtc
     5701 gctgtgatgt tccaccgttg aagcgtaaac cgtttgaccg atggatgatt gataattttc
     5761 tggggatgat tgacgtgggg aatgatggag aatcagagag aaagattgca gcgttacagc
     5821 atgaggttga aattgtcaca gccagaatca agaaagctac cgccctactt cttgagatgg
     5881 atgatattga tgaactaaaa attcagctta aggaactgaa ccagaagcgc acagaactac
     5941 agaccacgat tgataacatg aggcgtaagg cttcactcac tgataaggaa ctaccccagc
     6001 ttaaagatat tgaccttatg actaaagcgg gtcgtgtcga gtgtcagttg attttgtcca
     6061 agcatctaaa agggcttaca ttgggtaagg attcggtaac tgtaacgcta cagaacgaca
     6121 ctgaaatcac catacctaca aacccgttac cgctaaatga tgggtcacca atctttgaaa
     6181 ttgctgataa agagctactg gatattgacg cttatcaact gtaattttca ggggctatga
     6241 agcccctttt tatgtctcaa taagtgccct tcttgttagg gttcacagag tactttcgca
     6301 gagcattacc cggcttaagc acctttgaag cttgctcatt agcggcatct gagaaggtgc
     6361 tctcaccatt tttaacgcta acgctcttag agcacgttac acgggctttt agcgcttcat
     6421 ctgataactc acttgccgaa tagccaaaaa gctctttaca ggtcagcata gggcttccaa
     6481 ggctcgttgt agtgtttccg gtgttctttt tactggtagc accgccatca ttcgtggtag
     6541 cggtctttcc agtagcagcc gaaacatcag tctgacctac gattgaacga aactctacac
     6601 cttggaaggt catctgaata cgaagaccgt tagc
//
LOCUS       DAJTXQ010000183         6622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000183 DAJTXQ010000000
VERSION     DAJTXQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.183"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..605)
                     /locus_tag="OO515_003814"
     CDS             complement(24..605)
                     /locus_tag="OO515_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577634.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OO515_003815"
     CDS             complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OO515_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6577635.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4870..5874)
                     /locus_tag="OO515_003816"
                     /pseudo
     CDS             complement(4870..5874)
                     /locus_tag="OO515_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5859..6236
                     /locus_tag="OO515_003817"
                     /pseudo
     CDS             5859..6236
                     /locus_tag="OO515_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6275..>6622)
                     /locus_tag="OO515_003818"
     CDS             complement(6275..>6622)
                     /locus_tag="OO515_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6577636.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1644 a         1471 c         1494 g         2013 t
ORIGIN      
        1 acctcaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
       61 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
      121 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
      181 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
      241 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
      301 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcactc accagaaacg
      361 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
      421 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
      481 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
      541 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
      601 tacatattct ctacggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
      661 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
      721 aaactcaaga ccgaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
      781 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
      841 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
      901 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
      961 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     1021 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     1081 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     1141 tccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     1201 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     1261 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     1321 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     1381 gtcactgtat ccactagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccggc
     1441 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     1501 gaaggctttg taattaccgg acatatacga ttttgctgca ccggaagatg ctgtattttc
     1561 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     1621 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     1681 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     1741 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     1801 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     1861 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     1921 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     1981 gttatacatg ctggctgagg cagcaggagc atcaatacct ccggtataag aagtatcaaa
     2041 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     2101 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     2161 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     2221 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     2281 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     2341 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     2401 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     2461 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     2521 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     2581 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     2641 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     2701 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     2761 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     2821 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     2881 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     2941 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     3001 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     3061 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     3121 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     3181 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     3241 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     3301 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     3361 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     3421 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     3481 ctgcttgagc gttaattccc ctcctcctga gacagacagg tccttatctt ttataatcga
     3541 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     3601 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgag
     3661 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     3721 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     3781 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     3841 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     3901 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     3961 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     4021 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     4081 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     4141 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     4201 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     4261 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     4321 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcgccataac ttccatatat
     4381 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     4441 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     4501 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggatagc atttaattac
     4561 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     4621 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     4681 gtcagatatt gatcacttgg tgtttgtatt ttgaactttg cgatcaaaat ttgatctttg
     4741 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     4801 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     4861 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     4921 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     4981 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     5041 tttccaggca ttaaattctg cgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     5101 accgctggcc ggggtcagat ccacatcaaa tgcggtggtg aacatcgcgt tgtgccagga
     5161 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     5221 tgtctcaata ctacggggga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     5281 caggtccgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     5341 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     5401 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     5461 agccagagca atacccacat cagtgatcag tgccgcgcgg gcaaaggcct gtgatgtcag
     5521 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     5581 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     5641 agagaggcga tcaccgggaa gggccgccac agcagaaact cccagctgtg cggcggcatg
     5701 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     5761 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     5821 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     5881 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     5941 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     6001 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     6061 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tctgggagat gcgttccggt
     6121 atatcaggaa gcactgggct gcgttgtgca acttctgtga tgatggcctg gctgaggtag
     6181 ataataatgc agcagaaaga gcgcttcgtg cagtctgtct cgggaagaaa actttttcga
     6241 ggatcactca ttccgtgtct gactgcttca gggtttaatt ttgcttctgc tgactgaatg
     6301 atgattgggc agcaagtgca atatcccaca taaatccagt taactcacga gccaccgccg
     6361 taatggctac ttgtgatttt ttcccttttg ctgtcagagt tcggtaacga gaacacaatc
     6421 gttgttgagc tttccatgca atatctttgg aatattgtgt tacagagtcc ggtcggtaat
     6481 atataagcca tgctccaact tttgcaggtg tacgataaga ccaagcggct tcgtaaagca
     6541 gacgtctcag ttcgctgttt ccaacttttg ttattcctct gggacgaata ctgtttccac
     6601 tggaatattc acctggcacc ag
//
LOCUS       DAJTXQ010000184         6601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000184 DAJTXQ010000000
VERSION     DAJTXQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.184"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..337
                     /locus_tag="OO515_003819"
     CDS             <1..337
                     /locus_tag="OO515_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6577637.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="OO515_003820"
     CDS             337..666
                     /locus_tag="OO515_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6577638.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="OO515_003821"
     CDS             676..1374
                     /locus_tag="OO515_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6577639.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="OO515_003822"
     CDS             1379..2122
                     /locus_tag="OO515_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6577640.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="OO515_003823"
     CDS             2059..2661
                     /locus_tag="OO515_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6577641.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="OO515_003824"
     CDS             2722..6201
                     /locus_tag="OO515_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6577642.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..>6601
                     /locus_tag="OO515_003825"
     CDS             6269..>6601
                     /locus_tag="OO515_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6577643.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWF"
BASE COUNT         1504 a         1584 c         2104 g         1409 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgt
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 c
//
LOCUS       DAJTXQ010000185         6427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000185 DAJTXQ010000000
VERSION     DAJTXQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.185"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..613
                     /gene="gstB"
                     /locus_tag="OO515_003826"
                     /pseudo
     CDS             1..613
                     /gene="gstB"
                     /locus_tag="OO515_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(610..1725)
                     /gene="yliI"
                     /locus_tag="OO515_003827"
     CDS             complement(610..1725)
                     /gene="yliI"
                     /locus_tag="OO515_003827"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6577644.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1836..2219)
                     /gene="bssR"
                     /locus_tag="OO515_003828"
     CDS             complement(1836..2219)
                     /gene="bssR"
                     /locus_tag="OO515_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6577645.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2432..3757
                     /gene="rimO"
                     /locus_tag="OO515_003829"
     CDS             2432..3757
                     /gene="rimO"
                     /locus_tag="OO515_003829"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6577646.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(3804..5125)
                     /locus_tag="OO515_003830"
                     /pseudo
     CDS             complement(3804..5125)
                     /locus_tag="OO515_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5133..>6427)
                     /locus_tag="OO515_003831"
     CDS             complement(5133..>6427)
                     /locus_tag="OO515_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6577647.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1700 a         1618 c         1466 g         1643 t
ORIGIN      
        1 ggggtcggaa taattcaact aacgtaaaaa agtattgctg acgctcgaag aactggaact
       61 accttatgag caaattctcg cgggccgtga gtttgggata aatcacgatg ctgattttct
      121 ggcgatgaac cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg atctcattct
      181 ttgggaatca aacgccattg tccgctatct ggcggcacag tacgggcaaa aacgcctgtg
      241 gatcgactca ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg caaaccagac
      301 gctcagtaat gctcatcgcg ggatcctgat gggattagtc agaacaccac cggaagagcg
      361 cgatcaggcc gccattgatg ccagttgcaa agagtgcgac gccctgtttg ccctgctcga
      421 tgcggaactg gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg gcgatatcgc
      481 tatcgcaccg ttcatctaca atttgtttaa cgttggcctg acctggacac cgcgtccaaa
      541 tctgcaacgc tggtatcagc aactcactga acgccccgca gtgcgcaaag tggtgatgat
      601 tcccgttagc taattgcgtg ggctaacttt aagtaattcc ccactggact cgtcggtgag
      661 aacgtataaa taaccgtcgg gtccagtgcg aacatcacga attcgctgcc ctctgtccgt
      721 taaaatacgg ccatcttctg tcactttgtc gccgttgacg ctcatcacaa tgacatcttt
      781 atctttcagc gcgccaataa ataatttttg ctgccactgg gggaatttat cgctgttata
      841 gaaggccatg ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc
      901 ggcgacgatc tcccctttcg cttccggtat cttaaagcct gaatagttga ttccccaggt
      961 tgccagcggc cagccgtagt gtttgccttt ttgcgggata ttaatttcat caccaccgcg
     1021 cgggccatgt tcattcagcc acagtgcatt actccacgga ttcatcgcca ttccttgcgg
     1081 attacgaatg ccataagacc agatctcggc gcgcgcaccg gattccttta taaaaggatt
     1141 atcatccggg atttcgccct ggtcggtcag acgcaccagt ttgccctgta atttatccag
     1201 atcctgcgcc gtcgggcgct gattgttttc gcccagagca ataaaaagat aacctttacc
     1261 gtcgaatacc agccgcccgc caaaatggtt gccggtagac agttttggca tctggcggaa
     1321 gacggtgcgg aagtcggtca cttttgagag atcatcactt aagcggccat aacccacagc
     1381 agttccggct ttgccatcat cgccaacttc ggaataactt aaccagatgc ggcgagactg
     1441 agcaaaatca ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc
     1501 cggaactccg gaaagcggcg cagataatcc ttttcctgct tgccagtgac gcaactcgcc
     1561 gccgcgcaga gtgattaaca taccgtgatt atcgggtaaa aaggccagtg cccagggatg
     1621 gtcgagtttg tcttgcagta cttcgacatt taccgttgca ggagccgccc agagagcgga
     1681 agaaagacaa ataaggggca caaggaaaaa ggattgtcga tgcataacgc ctccggattg
     1741 actctggctt aaagcgtagt cagtggagga gataacaaat tcatttttac aaaaacttaa
     1801 acatgaaggg ggagacgctt tctccccctt agttttcagg ccttctcaag catggcgtgc
     1861 ttctgcaggc tctggatact cagcgttaag ctcatcagac aattttcaag cttatcggcg
     1921 ttgacggtaa taacagtcgg gcaatcatgg tgcccactca tcaaacatac tgcggctgtc
     1981 gctaatgctt cttcagcatg atgaagagca ctccactctt cctgatccag atgaagattc
     2041 aaccgcagcg atttatcgtg cagttcgcga ttcagtttaa aaaagttatc tcgtagatga
     2101 ttgctttcgc tgacggacat gtatcctttt gccttcctca gttgcacgta tgcagcgagg
     2161 tcaacgcgtg cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc aacgaacata
     2221 cttcgatcct cctctttcca gccttgttgc gattaaatag tatggctatt ttttgagcaa
     2281 aatgctgtcc aggatcaatt cttcacgcca aattaacaaa atttacattt ccttcgcctc
     2341 cgccagcgaa tgagattgcg atacaaattt tatccagaac tctgtagaat ccctgccctg
     2401 acctttcctg taactgaata ttttttaagc tatgagcaaa gtaactcccc agccgaaaat
     2461 cggctttgtt tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta ttctcaccga
     2521 actacgcact gaaggttatg acgtggtacc gagctatgac gatgcggaca tggtgatcgt
     2581 caacacctgc ggctttattg acagcgcggt acaagaatca ctggaagcca ttggtgaagc
     2641 gttgaatgaa aacggcaagg taattgtgac cggttgtctg ggggcaaaag aagatcagat
     2701 ccgcgaagtc cacccgaaag tgctggaaat caccgggcct catagctatg agcaggttct
     2761 ggagcacgtt catcactacg tgccaaaacc gaaacacaac ccattcctga gcctggtgcc
     2821 agaacaaggt gtgaaactga caccgcgtca ttatgcctat ctgaaaattt cggaaggctg
     2881 taatcaccgc tgcaccttct gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc
     2941 gattggcgaa gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta aagagattct
     3001 ggtgatctcg caggatactt ccgcctatgg cgttgatgtt aaacatcgta ctggcttcca
     3061 caacggcgag ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat cgaaactggg
     3121 gatctggaca cgtctgcact acgtttaccc ttatccgcat gtggacgacg tcatcccact
     3181 gatggcagaa ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg ccagcccgcg
     3241 cattctcaaa ctgatgaagc gtccgggttc tgtagatcgc caactggcgc gcatcaaaca
     3301 gtggcgcgaa atctgcccgg aactgaccct acgctcaacc tttattgtcg gcttccctag
     3361 cgagacggaa gaagatttcc agatgctact cgacttcctg aaagaagcgc gtctggatcg
     3421 cgttggctgc tttaaataca gcccggttga aggtgcagac gccaatgccc tgcctgacca
     3481 ggttccggaa gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc agcagcagat
     3541 ttccgccgag cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt
     3601 ggacgaagaa ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt
     3661 ttatctcaat ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca
     3721 cgccgatgag tacgatttgt ggggtagccg ggtttaatta attcaggccg ggcaaaatca
     3781 tttgtccgcc ctgcgaataa tgattactgc cagctaatct gagccctgcc gtttttctta
     3841 ctcttataca gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt acattcattt
     3901 gaatgcgcta caccgatact ggtacttaaa ttaatgcttc ccacctggaa atgaacgatt
     3961 tcttttagaa tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg
     4021 tcaccgccga agcggtagat tttaccttta tctaccggga tctgtgattt aaggattttc
     4081 accactttca tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg
     4141 aatttatcga cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct
     4201 tcgtcaaagg attttctgcc cattgctttg gtcaggtcgt ccaggaacgc cgagttatac
     4261 atactgtaga gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt
     4321 tcaaaatggt tattaatgat ggcttctttc tccacaatac cacgattaat cactctgcgt
     4381 accagataga ggcaactggt acccgatatc actgccacca atgcggcaaa gaaaataatc
     4441 aatgtacaga cccgcaaata aacatctttt ttatacgcaa atacgtacaa tccggtcggc
     4501 aaataggcga agtgctccat cgcttccgaa acggtgaaga tggcgttttt agcattatat
     4561 tcatcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg tccatcgcca
     4621 gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt
     4681 gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat
     4741 ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact
     4801 cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag
     4861 agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt tcctccgtgg
     4921 gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca
     4981 ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt
     5041 gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa
     5101 gtacgaactt attgattctg gacatacgta aactactctt ttactaattt tccactttta
     5161 tcccaagcgg agaatggcat gggtttctgg tacagatacc cctgagcctg aacacatcct
     5221 aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc
     5281 cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt accgaaaata
     5341 tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc
     5401 aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg tttacgcagt
     5461 tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca
     5521 acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga
     5581 aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc
     5641 agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat aatctgataa
     5701 cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg
     5761 atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg
     5821 ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga
     5881 atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc atcacagaaa
     5941 ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg
     6001 taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga
     6061 ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc
     6121 tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag
     6181 cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt attgcgttca
     6241 tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc
     6301 agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc gctttcctga
     6361 tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc
     6421 gcgatcg
//
LOCUS       DAJTXQ010000186         6399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000186 DAJTXQ010000000
VERSION     DAJTXQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.186"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            21..341
                     /locus_tag="OO515_003832"
     CDS             21..341
                     /locus_tag="OO515_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCR6577648.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            443..1897
                     /gene="amn"
                     /locus_tag="OO515_003833"
     CDS             443..1897
                     /gene="amn"
                     /locus_tag="OO515_003833"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6577649.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2228..2944
                     /locus_tag="OO515_003834"
     CDS             2228..2944
                     /locus_tag="OO515_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6577650.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3397..3472)
                     /locus_tag="OO515_003835"
     tRNA            complement(3397..3472)
                     /locus_tag="OO515_003835"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3437..3439),aa:Asn,seq:gtt)
     gene            complement(3573..5216)
                     /gene="yeeO"
                     /locus_tag="OO515_003836"
     CDS             complement(3573..5216)
                     /gene="yeeO"
                     /locus_tag="OO515_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6577651.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5221..5296
                     /locus_tag="OO515_003837"
     tRNA            5221..5296
                     /locus_tag="OO515_003837"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5254..5256,aa:Asn,seq:gtt)
     gene            complement(5334..6284)
                     /gene="cbl"
                     /locus_tag="OO515_003838"
     CDS             complement(5334..6284)
                     /gene="cbl"
                     /locus_tag="OO515_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6577652.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1822 a         1543 c         1373 g         1661 t
ORIGIN      
        1 actatcaata agttagagtc attaccatgg cgcttgaagc acaatctatt ttctggatag
       61 ttttcttctc cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac
      121 cgatgtttac cgaaatgttt ggtgccagtt atcgctatag tggcgctgga gtcggttatc
      181 aggttgccag tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt
      241 actttgccgg gaactggcat agcgtcgcca tttatttgct ggctggatgc ctgatttccg
      301 caatgaccgc tttgttgatg aaagacagtc aacgcgcttg atagcctggc gaagatcatc
      361 cgatcttcgc cttacacttt tgtttcacat ttctgtgaca tactatcgga tgtgcggtaa
      421 ttgtatggaa caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc
      481 actggataaa ctcgacgcgc tgtatgagca atctgtagtc gcattacgca acgccattgg
      541 caactatatt acaagtggcg aattacctga tgaaaacgcc cgcaaacaag gtctttttgt
      601 ctatccatca ctgaccgtaa cctgggacgg tagcacaacc aatcccccca aaacgcgcgc
      661 atttggtcgt tttactcacg caggcagcta caccaccacg attactcgcc ctactctctt
      721 tcgttcgtat cttaatgaac aacttacgtt gctgtatcag gattatggtg cgcatatctc
      781 tgtgcaaccc tcgcagcatg aaatccctta tccttatgtc atcgatggct ctgaattgac
      841 acttgatcgc tcaatgagcg ctgggttaac tcgctacttc ccgacaacag aactggcgca
      901 aattggcgat gaaactgcag acggcattta tcatccaacg gaattctccc cgctatcgca
      961 ttttgatgcg cgccgcgtcg atttttccct cgcacggttg cgccattata ccggtacgcc
     1021 agttgaacat tttcagccgt tcgtcttgtt taccaactac acacgttatg tggatgaatt
     1081 cgttcgttgg ggatgcagcc agatcctcga tcctgatagt ccttacattg ccctttcttg
     1141 tgctggcggg aactggatca ccgccgaaac cgaagcgcca gaagaagcca tttccgacct
     1201 tgcatggaaa aaacaccaga tgccagcatg gaatttaatt accgccgatg ctcagggtat
     1261 tactctggtg aatattggcg tgggaccatc aaatgctaaa accatctgcg atcatctggc
     1321 agtgctacgc ccggatgtct ggttgatgat tggtcactgt ggcggattac gtgaaagtca
     1381 ggccattggc gattatgtac ttgcacacgc ttatttacgc gatgaccacg ttcttgatgc
     1441 ggttctgccg cccgatattc ctattccgag cattgctgaa gtgcaacgtg cgctttatga
     1501 cgccaccaag ctggtgagcg gcaggcccgg tgaggaagtc aaacagcggc tacgtactgg
     1561 tactgtggta accacagatg acaggaactg ggaattacgt tactcagctt ctgcacttcg
     1621 ttttaactta agccgggccg tagcaattga tatggaaagt gcaaccattg ccgcgcaagg
     1681 atatcgtttc cgcgtgccat acgggacact actgtgtgtt tcagataaac cattacatgg
     1741 tgagattaaa cttcccggtc aggctaaccg tttttatgaa ggcgctattt ccgaacatct
     1801 gcaaattggc attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac tgcattcgcg
     1861 taaattacga acctttaatg agccgccgtt ccgataataa gttctgaacc ttcttatcag
     1921 aaatagatgt gaaggagtaa gtaagacacc tggcaaatag cctgcaataa tcgtgggctg
     1981 tttgcttcct tgggcggata cgagttttat tatcgtctta atgatttcca aatattaaaa
     2041 gcaagtatgc tttcaaaaca caattataaa aaatcccgcc aacaatataa gtttttataa
     2101 aattatggct ttagaatatt tttatttcta atagacgaga tttttcctgt tatgatataa
     2161 tatgccgaat taacacatgt taacgattta ccagtaatgt aaataaattt tcgaggagat
     2221 cattccagtg ggacgtaaat gggccaatat tgttgctaaa aaaacggcta aagacggtgc
     2281 aacgtctaaa atttatgcaa aattcggtgt agaaatctat gctgctgcta aacaaggtga
     2341 acccgatcca gaattaaaca catctttaaa attcgttatt gaacgtgcaa agcaggcaca
     2401 agttccaaag cacgttattg ataaagcaat tgataaagcc aaaggcggcg gagatgaaac
     2461 gttcgtgcag ggacgttatg aaggctttgg tcctaatggc tcaatgatta tcgccgagac
     2521 attgacttca aatgttaacc gtacgattgc taacgttcgc acaattttca ataaaaaagg
     2581 cggcaatatc ggagcggcag gttctgtcag ctatatgttt gacaatgctg gtgtgattgt
     2641 atttaaagga tcagaccctg accatatttt tgaaatttta cttgaagctg aagttgatgt
     2701 tcgtgatgtg actgaagaag aaggtaacat tgttatttat accgaaccta ctgaccttca
     2761 taaaggaatc gcggctctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga
     2821 aatgattgct caatctgaag ttgagctttc cccagaagat ttagaaatct ttgaagggct
     2881 tgttgatgcc cttgaagatg acgacgatgt acaaaaagtt tatcataacg tcgcaaatct
     2941 ctaattatct tttaaagaaa tctgtcttta cggcagattt ctttaatctc atataattct
     3001 tataaaaaat ataagattca actcgtcata ttgattatac ccccccgttc ccagagaaat
     3061 aatatttatt aaaattccag ttcttctttt tctgattaca gaaggcaaag tggcaattac
     3121 gcatagtttc ccgataaaga cgcgatagcg acatcccgca taaggcattt ttctctttat
     3181 ctttgtacgg tacttcatgg aacagagttt ttgaccttgc gaatcgtgat gtctgttggg
     3241 gaggggcaat ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca
     3301 aaaaacccgg tcactttttt acaaggtaac cgggtaaaaa taatttttat tttttaactg
     3361 ttttgagact catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac
     3421 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
     3481 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
     3541 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
     3601 cacttttgcg gttcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag
     3661 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca
     3721 ccccagccaa gcatgatgcc cagcacataa ccgacgacaa cccggcaacc ccacatgctc
     3781 aacatagaaa cccacatggc gtaacgggca tcgcgagcac ctttaaatcc agcgggtagc
     3841 acccatgagg cggaccaaat aggcataaat aaagcattta gccaaatcag aatcacaacg
     3901 acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
     3961 gttagccagg cgatggccgt taatccaaga gtggaaagcc agaacacatg ccgcaactga
     4021 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
     4081 gagccgagcg cacttccggg taagttgata agagccgcaa ttgaaaacgc gataaaattt
     4141 ccggcaataa cactggtccc catcccggca acgaacattt gggttaataa ccgaccactg
     4201 gtaaataaca ctgattcgac actcgcggga ataccaatcc ccatgacttc ccagataatg
     4261 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
     4321 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccagc
     4381 cctgccccga caaatcccag tcccggccag gagaaaaggc cgtaaatcaa tatgccgcta
     4441 ataataatat taagaatatt caggctaccg ttaatcaata gcggtatttt cgtattccct
     4501 gcaccacgaa gtgccccgct accaataaga gtgatggcag ctgctggata actgagtacc
     4561 gtcagctcca gataagtcaa cgccagtgct ttaacttctg tcgtggcatc acccgcgacg
     4621 aaatcaataa tttgttcgcc aaaatgatga ataagcgttg ccaacagtac ggcaaacaac
     4681 gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg
     4741 agactaaatg ccacaacgac agtagtacca agatcgatag cagcaaaaaa agccataatg
     4801 accatattga agctgtccgc caatcccacg ccggccatcg catcttttcc cagccagctg
     4861 accagaaaag tgctcagaac ccccatcaac aggacacagg cattctccat gaagatagga
     4921 acagcaagcg gggttatctc gcgccagaac aacactttgt agctcttgcg tttagcgtgc
     4981 cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat
     5041 tgcagaaagt gaaaccacat ttcaaataat gagggagaat cagcaaagct gcaaagattt
     5101 tcgccaacaa attgtctgca aatgcaacaa actgttgata gaaacggcaa acagttgggg
     5161 aatttaaaaa tcgggtttga caaaagattt ttcgccgtta agatgtgcct caacagcgat
     5221 tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt
     5281 ccagtcagag gagccaaatt tctgaaaagc ccgcttttat agcgggcttt ttgctatatc
     5341 tgataatcaa tttcctcttc actgttttcc atcacctgcc gcttgatatc ctcaactgac
     5401 agtcctgcat tacaaagttc cagaaagcgc cagacatagt tacgctgaag ttgtcctcgc
     5461 ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag acggattaaa
     5521 ttctcttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc aagcccaaga
     5581 gcaacatagg ttttaatgac atcagaatcc tgcgcactta atacaatatc tgccagcaaa
     5641 cctttgcggg caaatgcgtc atcaatacgt gagcgccccg taatcccctg tcggtaagtg
     5701 attaacggcc acttcgctat tgattccagc gtcaatggtg taatttgcgt taagggatga
     5761 tcgagtggaa caagcaaact atggtgccaa cgaaaccacg ggaaggcgac gagctgcggg
     5821 tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg caacaacgtc
     5881 gcaatttcct gtggcgtccc ctggattagc tcgagccgaa cctccgggaa aagttcgcga
     5941 aaagctttaa tgacctctgg caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg
     6001 agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt actggcttca
     6061 ttcagaatac gttctgcaat gaccagtaat gctttgcccg gttcagtcat gcccagcagt
     6121 cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat cctccagttc ccgaatatga
     6181 cggctgacgc cggactgtga ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc
     6241 tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa ctccgggcag
     6301 ttcagatttc ccgttattgt taaagtctaa tgcccggcat aacaaataat aaaaacccgc
     6361 atcttattcc atcccgatat aacgcttagc tcaccaatt
//
LOCUS       DAJTXQ010000187         6124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000187 DAJTXQ010000000
VERSION     DAJTXQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.188"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..675
                     /locus_tag="OO515_003839"
                     /pseudo
     CDS             1..675
                     /locus_tag="OO515_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(717..1169)
                     /locus_tag="OO515_003840"
                     /pseudo
     CDS             complement(717..1169)
                     /locus_tag="OO515_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1233..1379
                     /locus_tag="OO515_003841"
                     /pseudo
     CDS             1233..1379
                     /locus_tag="OO515_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1379..2536)
                     /gene="intS"
                     /locus_tag="OO515_003842"
     CDS             complement(1379..2536)
                     /gene="intS"
                     /locus_tag="OO515_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6577653.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2698..2772)
                     /locus_tag="OO515_003843"
     tRNA            complement(2698..2772)
                     /locus_tag="OO515_003843"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2737..2739),aa:Arg,seq:cct)
     gene            complement(2848..3780)
                     /locus_tag="OO515_003844"
     CDS             complement(2848..3780)
                     /locus_tag="OO515_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6577654.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4074..4829
                     /gene="mlaA"
                     /locus_tag="OO515_003845"
     CDS             4074..4829
                     /gene="mlaA"
                     /locus_tag="OO515_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6577655.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5011..5346)
                     /locus_tag="OO515_003846"
     CDS             complement(5011..5346)
                     /locus_tag="OO515_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577656.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5304..5696)
                     /locus_tag="OO515_003847"
     CDS             complement(5304..5696)
                     /locus_tag="OO515_003847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577657.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5703..6079)
                     /locus_tag="OO515_003848"
                     /pseudo
     CDS             complement(5703..6079)
                     /locus_tag="OO515_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1526 a         1362 c         1352 g         1884 t
ORIGIN      
        1 gtttctggag agaggttcat ctgtacttgt cgactctagc tctagagatt ttttcctgac
       61 atatcctgaa agagtgatag tggcggattt tggcgctgag tttattagtc gctatttgaa
      121 agctaataac ttaagggata tttctgattg tagggaatat ctatcttatt taaaaataaa
      181 ctttgctgac ttcagtttaa ttaaaggatt aattagttgg gctaatcact gtgctgaata
      241 catagaaatt tttgatgagt ctattgcttt tacatgtctc tctgcatttt cttctgaaaa
      301 acaatttgga gtatttctgt ttggatgttt gaaaagcaca gggctaaagt taaaacgatt
      361 attcatacgg atttatctgc accatggcgt cttaaggata tatcatcaag attatatctc
      421 agcgaaagtt tactaaagat gaaattgaaa gaagaggggg tatcattcag taagatcata
      481 cttgatgaga ggatgcaaat ggctgaatat ttactcagca ctcgttgtta tcctattagt
      541 aaagtagcta aggtctgtgg ttatgccagt gtctcatact ttacttatgt atttagacgt
      601 tattttggtg tttctccaag tcaatactct cagaggagtt cagaaagtaa aattcttact
      661 caccagggaa tctgatcatt gttcttgccc ccttatttcc agacaggggg tgtatcttaa
      721 gttaacgtta cccgctgacg tcgatattct cgcggagagc gataacccag cgcactgtgc
      781 ggatggtttt cattgtaatg ttcgatcgcc actgcaagat tatgcaatgc cgttcttaca
      841 ttcggtttcg gcatgaacgc gatgtagtct tccttcatcg ttttcacgaa cctttctgcc
      901 attccattac tctgaggact gctgattgcc gttgtgcagg gctccagatt caactctctg
      961 gcgaactgcc gtgtttcatg cgctctatac gctgaaccgt tatctgtcag ccactggatg
     1021 gactgttccg gtactttatc gccaaagcgt ttttctatcg ctcctaacat gacatcctgc
     1081 actgtcgctt tatcgtaacc tcccgtgctt gcggcccagt ctatggcttc acgatcgcaa
     1141 cagtccaggg cgaacgtaac acgcagtttt gaatcgccac gggtttaaca gacacctcag
     1201 agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat cccgaagagt
     1261 ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc agcgttgcaa
     1321 cacgtctcga tatcactacc cacagccttt atgcctggat aaagaagtac ggtccggatt
     1381 actccacttt ttcatcaagc caatccgccc accactgcat catttctctg cgcttatcga
     1441 gatactgagc atggttgtaa ataccgcgca cagatccgcc gttggcatgt gccagctgca
     1501 cttcaatagc gtcagcaggc cattcgtgct cgttcataat cgtgctgaat tcatgcctga
     1561 atccgtgacc gctttccaga ccttcatagc cgatttgttt gatcacaagc aataccgcgt
     1621 tctcgcagat tggcttcttc ttatcgttgc gcccggcaaa aacaaactct gagactggtt
     1681 tggtgattga gcttagcgta gtgagaagtt caaccacctg gtctgacatc gggaccacat
     1741 gaattttgcg tcccttcatc acactggcgt cgatggtgat aatcctgttt tcaaaatcga
     1801 cgttcttcca tagcatggaa cgaagctctt ttgttcttag ggctgtgtag cgtaaaactt
     1861 tggtcgcaat gagcgatacg atacttcctg aaaatgttgc cagtgctttg ttgaatgcag
     1921 gcatctggtc tgctggaagg aacgggaagt tcttcttgcg gtatcctttc atggcgtctg
     1981 caaggtcagg tgccgggtta tatttagccc ttccggtgac aatagcgtaa cgaaaaacct
     2041 cgccgcatct tctgcgggct ttgttggctc gctccattgc accgcgatct tcaaatctgc
     2101 ggattacttc cagcagttgc atcggctcaa tatcctgaat ctcaagaccg ccgatgatgg
     2161 gtaaaatgtc gtcatcaaac attttggcaa gttcagttgc atagcctact gaccagactt
     2221 gcttcttgtg ctcgtaccat tccttgtaaa tcgcactaaa tgaattgttg ttagacgaag
     2281 cctttttcgc ctttaccgga acgatgccaa ccgagatgtc tttcctcgca gtccatgcct
     2341 tatcccttgc ttcctgcaaa gtcataagcg gatattttcc gacggtcagg attttctcct
     2401 taccgtcaat cttgtagcga agctgccata cctttttccc ggatacagga acataaaggt
     2461 acaggccatt accatcgaga aggcggtatg gtttttcttt cggctttgct gcttcaatct
     2521 gcttaacggt gagcatgggt aaaaatccgg tgggtaaaat tattttatcc actttttacc
     2581 cgtcatggag tgcggctgtc aacgatctga cgcgaaccat gacgaaccgt gaatatacgg
     2641 aaggcttgat attcagggga ttttgcggac tggtacggat gggagcgaac tgataaatgg
     2701 tgtcccctgc aggaatcgaa cctgcaatta gcccttagga ggggctcgtt atatccattt
     2761 aactaagagg acaatgcggc atgagtatac ccgctaatgg agtgcggggt aagtacgctg
     2821 ccgctcgatt gcttaaaccc tcgccattta tgccgggttt ttatcatttt tcttaatgtt
     2881 ttccgcacgt tctgcttttt ggcgtgcttc tgctttacgc ttgttgctca tgtcgttacg
     2941 aatctgtgca tgactcatta acgcgaagat aaaggtgccg ccgcagatgt tccccgctaa
     3001 agtaggtagt gcgaagggcc agatgaaatc gctccagtgc agcgtaccgt taaacaccag
     3061 atagaggatt tcaacagaac cgaccacgat atgggtggtg tcacctaggg caataagcca
     3121 ggtcatcaat ataatcacca caatctttgc cgcacctgct gcaggaaaca tccataccat
     3181 agtggcgatc agccagccgg aaatgatcgc gttggcaaac atctcgctgg gggtgttctt
     3241 catcacatcc atgccgattt tgacaaatgc atcgcgagtt tcttcattga agataggcat
     3301 atattcaaat gcccacgcag caataccagt cccgagaata ttacccagca gcacgactcc
     3361 ccataaccgc ataagtaagc caacgttgct cattgtcggt ttttgcatga cgggtagtac
     3421 cgcagtcacg gtgttttcgg taaataattg ctggcgggcc attatgacga taataaaacc
     3481 aaaggtataa ccgagattct ccagtaagaa gctgcccggc acaccttcca gttcgacatg
     3541 aaatatccct tttgccagta acgaagcgcc catcgacaga cccgccgcaa tggctgacca
     3601 cagtagcgcc attgcgtcgc gttccagctc tttttcacca tcctggcgga tatgctcatg
     3661 aattgccatc gcccgggagg ggagtcggtc ttcatctatt tctatttttt tgccgcgctc
     3721 tttttcttcg ctctcaactt caatttcgtc gctgtgttga tcaattttgt cgttgtccat
     3781 ggtctcttct cttgaattag cacgtatagc taaagcgtag cggctttttt gctcgcaact
     3841 ggcgggagtt actctgaaaa tgtagaaaag gctgcgtttg cctttttctg tttctataga
     3901 atcaagtagc ctacagggcg gcgattacca ggctatgatc aaatcagcaa atcagggcgt
     3961 ctggacatca gttgacgtgc tgttacaatc gcccacacct aaacaggcgg atacggtatc
     4021 gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa cagggagaca tttatgaagc
     4081 ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt ggggtgtgcg agttccggta
     4141 cagatcagca agggcgttct gacccgttag aagggttcaa ccgcaccatg tacaacttca
     4201 acttcaacgt attagacccg tatattgttc gaccggtcgc tgtcgcctgg cgtgattatg
     4261 ttccgcaacc ggcgcgtaat ggtttgagca actttactgg caaccttgaa gaacctgcgg
     4321 tgatggttaa ctacttcttg cagggcgacc cttatcaggg gatggtccac tttacccgct
     4381 ttttcctgaa caccattttg gggatgggcg gctttattga tgttgcaggg atggcgaacc
     4441 cgaaactgca acggactgaa cctcaccgct tcggtagtac gcttggtcat tatggcgtgg
     4501 gttatgggcc ttacgttcag ttaccgttct acggtagctt cacgctgcgt gatgacggtg
     4561 gtgatatggc ggatggtctt tacccggttc tttcctggct gacctggccg atgtctgtgg
     4621 gtaaatggac gcttgaaggg atcgaaaccc gcgctcagct gctggattcc gatggtctgc
     4681 tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc gtacttccag cgtcatgatt
     4741 tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc gaacgcacaa gcgattcagg
     4801 atgatttaaa agatattgat tctgaataag aaacaaataa aaaaggtgag tcgcaaaact
     4861 cacctttttt gttgttatcc ctcaattacc acaagtaaaa ggagataaac cgacaatgac
     4921 ccggacgtaa tgatgtaaaa atactgatgt tcgtcactga ctatttccga tattcaggcg
     4981 ttgaaaaatc aaaaaactgg aaaaataatt ttaaatatca tcaaaattaa cacctggcgc
     5041 agatagttga ttaatttcat tttgatattt cgcctggcga ttttgaagtc ttttttcaac
     5101 gttatcttcg agatccttaa agtctttgta tttattttca ggatggtcaa cgtcaacttt
     5161 gttagcgaat aaattagaaa ttatttcttt tacattgtcg cgtctaaatt ttgcatcgtc
     5221 cacttgaaaa ttatatctta ttgctcgacc taaacgttta aaaacttcgt taggttctct
     5281 cagtctttcc ccgttgagct cgtttacaac ctgatgatcg gaacctgttg caaatgattc
     5341 tgccatgata gctttactgc tttcaaacca cgctgaagca ttgctgaagc attcctggct
     5401 ttttcactac aatattgggt gagactatca ccattaatac gaattcctct ttgaatgtgg
     5461 tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt atttttaatt
     5521 acaatagcga gagagttgcc ttttttatct ttaccgtacc aggtaaataa gtccgttggt
     5581 tcgctattcc cgctctcatc aaattctgtt accgtatgtc tgagtttgtg tgatatctta
     5641 tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa gatcatttcc
     5701 ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt acggacgagt
     5761 tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat accaatagta
     5821 atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt attaatatta
     5881 aatcttaagg atcgtcgttt actcgtatgg cgttttttat aagctcaagt agatgtttaa
     5941 tacaggtgct tattccttcc ttaccattaa aaaggttttt gagctcttgt actaatccat
     6001 taacttcatc attgttattt tcattaatgg attctgggta agttgcgctg gtattgttga
     6061 ttgaaataga tggtagcatg attttattcc attttgattg atttgttaca aatatatatt
     6121 acct
//
LOCUS       DAJTXQ010000188         5977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000188 DAJTXQ010000000
VERSION     DAJTXQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.189"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..215
                     /locus_tag="OO515_003849"
     CDS             <1..215
                     /locus_tag="OO515_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6577658.1"
                     /translation="RCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHA
                     VLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(255..926)
                     /locus_tag="OO515_003850"
     CDS             complement(255..926)
                     /locus_tag="OO515_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6577659.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1033..1266)
                     /gene="yedF"
                     /locus_tag="OO515_003851"
     CDS             complement(1033..1266)
                     /gene="yedF"
                     /locus_tag="OO515_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6577660.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1263..2468)
                     /gene="yedE"
                     /locus_tag="OO515_003852"
     CDS             complement(1263..2468)
                     /gene="yedE"
                     /locus_tag="OO515_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6577661.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2655..3068
                     /locus_tag="OO515_003853"
     CDS             2655..3068
                     /locus_tag="OO515_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6577662.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3102..4589)
                     /gene="amyA"
                     /locus_tag="OO515_003854"
     CDS             complement(3102..4589)
                     /gene="amyA"
                     /locus_tag="OO515_003854"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6577663.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4667..5032)
                     /gene="fliT"
                     /locus_tag="OO515_003855"
     CDS             complement(4667..5032)
                     /gene="fliT"
                     /locus_tag="OO515_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6577664.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5032..5442)
                     /gene="fliS"
                     /locus_tag="OO515_003856"
     CDS             complement(5032..5442)
                     /gene="fliS"
                     /locus_tag="OO515_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6577665.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5457..>5977)
                     /gene="fliD"
                     /locus_tag="OO515_003857"
     CDS             complement(5457..>5977)
                     /gene="fliD"
                     /locus_tag="OO515_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6577666.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1467 a         1570 c         1481 g         1459 t
ORIGIN      
        1 agcgttgcgc gtgctgtggt catacagcga aagaaaaccg cctgtcacaa agtaaattca
       61 gatgccaggt atgtggatat acagtgaacg ccgatgtaaa tggcgctcgt aacattttag
      121 cggcggggca cgccgttctt gcctgtggag agatggtgca gtcaggccgc ccgttgaagc
      181 aggaacccac cgaaatgatt caggcgacag cctgaacgta gcagggatcc tcgtccttca
      241 gggcgtggag gatgtcaaca aacaggttga attaactccg cgccctggtt tttgacatta
      301 cctaccgcgc gcgataccgg gtgccaggtg aactggtttg ctggcacaca gccgttggta
      361 gcaatttctg atgcttcttt accgccaatc tcttgccgca tccattcctg tgcagcttct
      421 ggcgacagaa ccagcgggcg gcggtcatga atgtctacca gaccctggtc tgctgcagca
      481 gtgacaatta aaaatccttc ggcttcgtca ccacgctcaa atggtgtgct acctatcgcg
      541 gccataaaaa taggttgtcc atcagcacga tagataaaaa aaggctgttt tttgtcgcct
      601 tcttttttcc actcaaacca gccatcggca aaacagattg cccgaccatg ttgccagagc
      661 ggtttaaaca tgcgactggt tgcggcagtt tctacgcggg cgttaatcag cggcggttta
      721 tcccaccatc cgggagcata tccccagaaa accggatcca gatgaaggtg ttcatcacgt
      781 tcactgagca gaagaacttt ggttcccggc gcgacgttgt atctgccaat gggttcggga
      841 tcgtagggaa tatcgcgttc aatatcttcc gcgagaagcg caaggtaatc ttcacgcgtt
      901 tgggattggg caaagcgtcc acacatagaa acctcctgag atcatactga aaagaatagg
      961 agagggggcg ggtaaaatct atgtgtcaga ggcaacaatg ttcccgctgt gggccacagc
     1021 gggaatgagg gattacttct gaattaaata acgaatggtc ggcccgtctt gctgaatatc
     1081 cagcaccgta tagccgtgat tacgcgcatc cagtggaata ttattgatcg actgtggaca
     1141 gtcgctcacc acttccagga tttccccttt ttttaactgc ggcatcgcct caagggttgc
     1201 gactgccgga taagggcagg gttcacccac catatcaagg cggtaatcag gaacgatatt
     1261 tttcatgcga tctccttagc agtctgcggt gccgcacggc ggaagaagcg tttttcccag
     1321 ccgataatca acatcagcgc agtaaacaac agcaaatatg tcaccagcag gccacccatc
     1381 gggccaaagg ttttcagcag gttgattttg tcccagtcgg tggccagcgc cggagcgaaa
     1441 tcatcccagt aatacgccag aatcgtcgag ccgatcacat tgcccagacc gacccaccag
     1501 tagtgcacct ggccttctac cgcgcggtac atccagccgg tttcgcagcc gcctgccagc
     1561 acgatgccaa aaccaaacag taaaccacca attaccgcgt ttggtcccgc ccacatgatt
     1621 ttgggttcaa cgcctaactg tacgtaactg aagatcccga tggcactcac cgccataccg
     1681 ataatgattg ctttcgccat atgggtacgt ccggtgatcc acatatcgcg gaacgccgaa
     1741 gtaaagcaga tttgcgcacg ttcaatcagt aaaccaaagc cgacgccaaa cagcattgcc
     1801 agccccagtt tgggttggtt catcgccgtg agcagcgccc agcccagcat gccgaaaaag
     1861 accagcatcc cgagacgaaa acgacgccgc gcctgatccg gtttttgcgt cagcggtgag
     1921 gcggcagaaa ctttctgcat tttcacggga atacggaaga tgggcagcag ggtaaagcgc
     1981 gcgccaaacc atgaaccaat ggcagtggcg atggcaaaga accaggcatg cagcgagaac
     2041 tgaggaatgc cggtaaagaa cgccgccagg ttacagccca ttgccagacg cgcgccaaaa
     2101 ccggcgataa tgccgccaat aatggcctgc ataatgcgga tacggctgcg cggcatgcgc
     2161 agtttgacat tgttggccca cagcgctgcg gcaaagcagc cgccaaacat gccgaggatc
     2221 atcatcccgt cgatgcgggt taatggcgat ccttccagat ggataatttt aaaataaccc
     2281 cactcttcag catggacgcc aaacagctgc aggagctgac cgccccaacg ggtaaattca
     2341 cccgtgacag cccaaaaggt gccagtaatg ccaaaatagt aagtggagag aatacccgcc
     2401 gcgatgaccg cagggatggg tgcccagaat ttaatcaacc aggcgtgttt gaattgctgc
     2461 catgacatga atgtagcctc tgaactcaga aggtttgaaa caagaatcgc gaatcataca
     2521 tcatgcaaat ttattttcgg ggtaataatt cacaaaaata atagttagat caaatttctc
     2581 tctgaacctg gaaaggctgg cgacatcagg gctttaatgc cacaatcctc ttttcttctc
     2641 atgcaggacg aatgatgaaa aaactcgcaa ttgcaggtgc gctcatgttg ctggcgggct
     2701 gtgccgaagt cgaaaactat aacaatgtcg tgaaaacccc cgcgccggac tggctggcgg
     2761 gctactggca gaccaaaggg ccgcaacgtg cgctggtcag tccggaagag ataggcagcc
     2821 tgattgtcac taaagagggc gataccctgg attgccgtca gtggcagcgg gtgattgccg
     2881 tgccgggcaa gttgacattg atgtctgatg atctcactaa cgttaccgta aaacgtgagc
     2941 tgtacgaggt tgagcgtgac ggtaacacca ttgagtacga tggtatgacg atggagcgtg
     3001 tggatcgccc gacggcagag tgtgccgccg cgctggataa agcgccgtta ccgacgccac
     3061 tgccgtaatg aaatctcccc ggcttgccgc cggggatgaa tttaaatcac ctcttcgata
     3121 acccacacgc tgacgctgcc gccgttgcaa aagaaggtcg cttcgccgtt ttcgtcggtc
     3181 actacgctct cttgccgatt ccccagaaaa tcacgccagg ttttattgcc gtaattctct
     3241 cccagatgaa tggttttttc gccatcatcc ccgttcgaca tgaccaccac gcagccggga
     3301 tattcgtcag tgccactgcg gctaaaggca atgcagttcg gatggtcgaa aaataacgtc
     3361 tgtacaccgt gggcgaaacg ctgacgggcg agaattaact catcaagctg ttcgattatt
     3421 ggcatatcta tcggataggt ttgcccgtca ccaccgacat cttcgtaatg cgcaccgtag
     3481 aggtccggat agaacaccga aggaacgcca ttttcccgca acaaaattag agcatacgcc
     3541 agcggtttaa accacggttc gaccggcgct tcgagggctt gcaacggttg ggtgtcgtga
     3601 ttggctacga gggttacggc gtggaaagga tcggcttcca ctaatgtacc cgtgaaaatc
     3661 tgcgtcatgt cgtagtcgcg tcccatgcgc gatgcttcat ggaatttcat ctgcagcggc
     3721 gcatcgaaca gcatggtttt gccttccacc tgatcaatat acgtttgcag tttatcgact
     3781 tcatgcgacc agtactctgc cacaataaac agcggctttg gcgcaacttc ctgtacgtgt
     3841 tcgatccact ctttataaaa ccacgccgga atatgtttaa ccgcatcaag acgaaaaccg
     3901 tcgcattgtg tttgttccat cacccagcgc gcccagtatt taatctcttc cgtcacggca
     3961 tgattgcgga aatcgatatt ctcacccatc agataatcga aattaccgag ttcatcatca
     4021 acctgatcgt tccagccttc gccggtgtag tcgttaacaa ttttaaatat gccatcttca
     4081 tcaggatttt cgatatggtc gataccgcta aaacatttga agtcccagat aaactgcgag
     4141 tattgcccgg cacgggcggg gaaggtgtaa cgcgtccagc cttcgcactc aatgatttct
     4201 tcatcaattt gcgtacggtc atcagcattt acacgctgca cgcgaatagc ttctttttca
     4261 tccgccccca ttttgtggtt gactaccacg tccagtagca ccgcaatgtc attacgtttc
     4321 agagcgtcaa tggcggccag cagttgtgct ttatcgccat atttagtagg gatgctgcct
     4381 ttctgatcaa actcacctaa atcaaataaa tcataggagt cgtagccgac cgaatacccg
     4441 cccgatgcgc ctttataggc gggcggcaac cagaccatat tgataccaat atcattaaaa
     4501 ccgtcggcgc gctcggccag ttcaggccag agcttaccgc cttccgggta ataccagtga
     4561 aaacattgta acagcgtggg attacgcatt cttcgactcc attcaagggg aacattagaa
     4621 gcgtagccgt aatcggatta ttcgcgagcc atcggctcgt taagattcaa aagaggttat
     4681 cctgcggagc cagcacaaag ccgccctgat ctccataggc acttaacact gatttttgca
     4741 ccgatgactg accgaccagt ttcgccagtt catccatacg aatctgtaat aactgcttta
     4801 ccttgctttc gttgtcgaga atcaggcgca gcatcgggcg gagctgctcc tgcatcgtgg
     4861 tggacggggc aacctcttca gtcagatgtg caatctcctg caccgcattc acatacgcca
     4921 tttcgctggc gatgagttcg tcccattgtt cttccgttgc caggcgtaac atgagctggc
     4981 ttttatcgac gagttgttgc caggcgaaat acaaatgcgg tgcatggttc atcagactgg
     5041 gtcctgaatc aaagaagggg agagtaaaga ctctttccag gcatcggcaa tattgcgcat
     5101 taatgcttcc acttcttcga ctgcggagac atcgttgcgt aaattggctt gcagcaagcg
     5161 cctgaccata tagctataaa gagcaatcaa gttttgggtt agttcgtctt tgctctcttc
     5221 atcaagactc acccgcagcc cgttctcaat gatgttgatc gcttttgaca aagagacgcc
     5281 tttgccttgc tgattgttgt cctgcataaa caggcgcgct ctaaccagtg cgctcagcac
     5341 tccatcaaat agcatggtga ccagctgttg ctggctggcg ctcattacgg cgctttcgac
     5401 gccaatttgt gcataggctt ttgagccgct cgcggtatac atgctgacct ccgtgatcag
     5461 gaattactca ttgcagtaaa ttgttggctg agataggtac tggtgttatt cagtttgctc
     5521 atcatggtat ctaattgcgt aaactgtgca gtgtaacgcg ccacggtgtc atcaatgctg
     5581 gcactgacgg atagatactg tttggtcagt tttttcagtg tggcgttaat actgtcctgt
     5641 gcgctatcaa taatgccatc atcggcaagg taacctttta cctcggtggc aattttggtg
     5701 gtaattcctg tttctttacc atcgcctacc agcaattcac gtacggatgc tgtattttca
     5761 ttcaggactt ttttgagttt atcatcatca attttcagct tacccgtcgt gccgtcctgc
     5821 ttaatcccga tctcgtttaa tgttttgaag gcactgtcgc tggcaccgtt tgcaaattgt
     5881 gcgcggatgc cgctttgaat tgtacgtaca acgctgtctc cgagaagtgc accattattt
     5941 ttatcttgtg cttcagcacc tgggtctact tctttat
//
LOCUS       DAJTXQ010000189         5975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000189 DAJTXQ010000000
VERSION     DAJTXQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.190"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO515_003858"
     CDS             complement(<1..202)
                     /locus_tag="OO515_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6577667.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
     gene            718..1287
                     /locus_tag="OO515_003859"
     CDS             718..1287
                     /locus_tag="OO515_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577668.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2015..2188
                     /locus_tag="OO515_003860"
     CDS             2015..2188
                     /locus_tag="OO515_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6577669.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO515_003861"
     CDS             complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO515_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6577670.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO515_003862"
     CDS             complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO515_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6577671.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4300..4890)
                     /locus_tag="OO515_003863"
     CDS             complement(4300..4890)
                     /locus_tag="OO515_003863"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6577672.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO515_003864"
     CDS             complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO515_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6577673.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5708..>5975)
                     /gene="rutC"
                     /locus_tag="OO515_003865"
     CDS             complement(5708..>5975)
                     /gene="rutC"
                     /locus_tag="OO515_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6577674.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1608 a         1536 c         1446 g         1385 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
      301 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
      361 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc acctcttcaa
      421 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
      481 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      541 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      601 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      661 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      721 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      781 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      841 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      901 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      961 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     1021 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     1081 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     1141 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     1201 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     1261 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
     1321 atggggactt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
     1381 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     1441 cctgaaaagg ggcttcacag attctctata tatttcaata agttaggata aaaagggact
     1501 ataccgatac tgcatagaat ctgtgtagga ttgcatagtc ataggggttg gaacaagccc
     1561 aactacacct gataaagcct gttattaaca ggtagttgaa tgtacgtgtt tagctatgta
     1621 aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat tccgtagcga
     1681 ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt cacaaccact
     1741 tatttgattt ataacaactt tcacaagcac gtaattttgt cgcaaaatga cacattttta
     1801 tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga gatgatatct
     1861 attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtctacccg cgtcagttca
     1921 ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt taattctatg
     1981 caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag gcggttccgg
     2041 caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg gacagcacag
     2101 cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa
     2161 gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg catttttgtc
     2221 tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg gttgatactg atgacaaatg
     2281 taagcttgcc tgatgcgcga tgcttatcag gcctaccaga agattgcaat atattgaatt
     2341 tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa caatgcgtac
     2401 gttgtcaaca atctgcaccg ccgggtaacc ccggcggttt tctgtttatg gctcctgatg
     2461 aacaacttct ggcggtggaa cgtcaaccaa ccttctactt aacaacgcat tgagtaaaat
     2521 cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa
     2581 atcacccgcg cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa
     2641 atcgacacgg ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac
     2701 aattgatgca ccaccaatca ccgcggccgg aatggtatgg atcagcgcgc caaatttcgg
     2761 cgaaaagccc aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac
     2821 ttttgtcacg gccatcacac cgatattttc agcataggtg gtgacgccgc taccgccgac
     2881 agagccggaa agcatcgttg ccagaccatc accgacgaat gctcgcccca tatacgggtc
     2941 catattgcgt ccggtcatcc cggcgaccgc cttgagatga cctaagtttt ccgccaccag
     3001 aatcaccgcc acgggcgcaa tcagcatcat tgcctgacta ttaaaagcag gagtggaaaa
     3061 atgtggcaaa ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag
     3121 ccctaaaacg ttggtcatca cgccatacag cagacaggcg acaattaaac ctacgagaat
     3181 caataaccgc tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt
     3241 cattaccgcc atccagctat caaaggctga agccgataca cttttcactg cgataggcgc
     3301 taagttcagg ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc
     3361 aatccagcgt gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca
     3421 ggcgataatc ccgcccagtg cgatgctgat attcgggtta atgccctgac cgttaaagcc
     3481 cgtcgcggca atcaccacgc cgacaaaagc cgcgctggag ccgagataac tggggacgcg
     3541 cccgccggta ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt
     3601 gggatccagc cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg
     3661 ttgaacgccc attactgccg tctgagcaaa cggcaatcgt tcatccggcg cgaccacgcc
     3721 gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg
     3781 tctccttaag gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac
     3841 accagcccgt agggtgtggt gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg
     3901 tgggtgccga cgctcaccac ctggctgatg cggcagtcaa acgaaaccag agcctcttcc
     3961 agttgcgggc atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg
     4021 ggcgttttgc cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca
     4081 cacagcgttc gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc
     4141 agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc
     4201 ccggatggac cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg
     4261 cgaaaggttt gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc
     4321 ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg ctatcgccat agccgatatt
     4381 aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtgttgacg
     4441 gtcaaaaccc gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata
     4501 ggccgcctgc atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt
     4561 aaaccaactg cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact
     4621 gtcccaggca acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga
     4681 aagcgccggg tgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg
     4741 agaacagtta gctgatgtcg gcccccattt tatcggggca taaagctccc gtaacgtctc
     4801 atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct
     4861 aagcgcacct gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcacggtg
     4921 atgtaacagg ctggcaagcc cgttgagtaa cagagcatta aacgtttcgg ggtcggtcac
     4981 gttgcaggcg tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg
     5041 aagctcactg gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac
     5101 cgggcagcgg atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg
     5161 acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag
     5221 gcgaggtgcg cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac
     5281 ccatgcctgc gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt
     5341 atgggcgttt attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata
     5401 atccagcgcc agctgcattc ccaccagcgc accgagcgca tggccgacca ccgcgtaatg
     5461 ctcaatccct gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta
     5521 atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg
     5581 atactcctgc tccagcaccg ccagttgcgg taaccagtaa ctgccgctac cgccaagacc
     5641 cgaaatcaac accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat
     5701 cgcggcctca cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta
     5761 ccagtccgca ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga
     5821 tttcgttaat cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat
     5881 ccgccatcgt gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg
     5941 tttgcgcctt tgggtcatcg gcaaacagca cgtta
//
LOCUS       DAJTXQ010000190         5962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000190 DAJTXQ010000000
VERSION     DAJTXQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.191"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..128
                     /locus_tag="OO515_003866"
     CDS             <1..128
                     /locus_tag="OO515_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577675.1"
                     /translation="HPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(261..773)
                     /locus_tag="OO515_003867"
     CDS             complement(261..773)
                     /locus_tag="OO515_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6577676.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            968..1732
                     /locus_tag="OO515_003868"
     CDS             968..1732
                     /locus_tag="OO515_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577677.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1683..3086
                     /locus_tag="OO515_003869"
     CDS             1683..3086
                     /locus_tag="OO515_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6577678.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3086..4552
                     /locus_tag="OO515_003870"
                     /pseudo
     CDS             3086..4552
                     /locus_tag="OO515_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4443..5186
                     /locus_tag="OO515_003871"
     CDS             4443..5186
                     /locus_tag="OO515_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6577679.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5190..>5962
                     /locus_tag="OO515_003872"
     CDS             5190..>5962
                     /locus_tag="OO515_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6577680.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDS"
BASE COUNT         1585 a         1416 c         1627 g         1334 t
ORIGIN      
        1 gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca ccgtcgaact
       61 ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca gcgaccagac
      121 agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt agcggtaatt
      181 ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag ctggattcgc
      241 aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa actaccaaca
      301 taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt cactggatga
      361 agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat aaagcgcgaa
      421 gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc tattgtattg
      481 gaacggcttg atattataca tgcaagttga gggatatgcc caattattat accaagatgg
      541 taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc ttcccatggt
      601 gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc tggtggcaat
      661 atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag cccccatagg
      721 cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat caatgtcttt
      781 ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag gcttaaacac
      841 cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca ggagtgagtg
      901 atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa tcacaggaag
      961 cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg ccggagtcct
     1021 tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca ggaagagggc
     1081 caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg gtacgcaaaa
     1141 aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa aaagtacgca
     1201 actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta ccgatccccc
     1261 gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt atgcccgtcg
     1321 ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg aggacgaatt
     1381 atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt ggttggtgtc
     1441 gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata tcagcgccgc
     1501 cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca cgcttgcgac
     1561 ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata aggtgtctct
     1621 ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag agcgtgacct
     1681 caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt agtttctgga
     1741 ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa tcatgggatg
     1801 ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt tgtgcccgtc
     1861 agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag atagacaggt
     1921 ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg gttactggct
     1981 cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca ctggagggga
     2041 tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg aaaattctgg
     2101 agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc ggacttgtta
     2161 ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc atccgcaaaa
     2221 ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc gacgcggcgc
     2281 gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag tctgatgatg
     2341 atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac aaaacgttaa
     2401 atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt ggtacagata
     2461 agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa tggaaggcca
     2521 aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg gagcgtgaag
     2581 cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa ttctctgaaa
     2641 ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac cagaggttta
     2701 acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc aacaaagact
     2761 ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc agaaatacgt
     2821 ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc atagattctc
     2881 gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt gatttcgacc
     2941 gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag ataccgtcac
     3001 caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg ctggatatct
     3061 gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc ggcgaactgt
     3121 gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc agacacctaa
     3181 ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca tgctggtgga
     3241 atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc cggatgatat
     3301 gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg gcattctcga
     3361 atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga aatggtcgcg
     3421 cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa tgaccccgct
     3481 gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg accgctggat
     3541 ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg gtaaacctga
     3601 gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc atcacagtcg
     3661 cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg agaacgaatg
     3721 gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca gcgctactgt
     3781 cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg agaagctacg
     3841 cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata tcgacctgat
     3901 tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata agtttgaaac
     3961 ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag aacagattag
     4021 tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag gattttctac
     4081 cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg agaggcgttt
     4141 acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg gcaagccgct
     4201 gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg tcgatcgctc
     4261 aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg gtctgatgac
     4321 actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca tcggggcatc
     4381 cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca tcggcgaacc
     4441 tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc ctacgcagga
     4501 tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat gacagtatca
     4561 cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg gcgacaaagg
     4621 tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg cggcaacaca
     4681 gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg ggccaggtga
     4741 tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc gaggccgcag
     4801 acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt ggtcgggcgg
     4861 agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg gccgatctga
     4921 ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga gcgatagcta
     4981 ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga gattcccacg
     5041 atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac ggcatgaccg
     5101 gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt gttcgcgttc
     5161 cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc aggctcgggg
     5221 aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg ataggacgaa
     5281 caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat aagttcggtg
     5341 aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt gcctcattcg
     5401 aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg cggctggtaa
     5461 atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg cgcgggacgg
     5521 gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc gccattcagc
     5581 ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac gagcagaccg
     5641 agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt gtccctaaag
     5701 gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat caaaagaaaa
     5761 actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc atgaacgaac
     5821 tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg cagggcgtta
     5881 atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca gagatgggcg
     5941 gagatccaac cggcgacagt ga
//
LOCUS       DAJTXQ010000191         5933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000191 DAJTXQ010000000
VERSION     DAJTXQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.192"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..309)
                     /locus_tag="OO515_003873"
     CDS             complement(<1..309)
                     /locus_tag="OO515_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577681.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     N"
     gene            1005..3290
                     /gene="nrdA"
                     /locus_tag="OO515_003874"
     CDS             1005..3290
                     /gene="nrdA"
                     /locus_tag="OO515_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6577682.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3524..4654
                     /gene="nrdB"
                     /locus_tag="OO515_003875"
     CDS             3524..4654
                     /gene="nrdB"
                     /locus_tag="OO515_003875"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6577683.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4654..4908
                     /gene="yfaE"
                     /locus_tag="OO515_003876"
     CDS             4654..4908
                     /gene="yfaE"
                     /locus_tag="OO515_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6577684.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4985..5611)
                     /gene="inaA"
                     /locus_tag="OO515_003877"
     CDS             complement(4985..5611)
                     /gene="inaA"
                     /locus_tag="OO515_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6577685.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            5790..5933
                     /locus_tag="OO515_003878"
                     /pseudo
     CDS             5790..5933
                     /locus_tag="OO515_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1519 a         1544 c         1405 g         1465 t
ORIGIN      
        1 gttattggca aacagagtca ttccagtttc gttttctggt gataatgtaa attccgctcc
       61 ttgttgcaaa aatacggccc caccgctggc attattggtc atatccgaaa aaaccagcca
      121 ttgcccatcg gcgatactcc agtcctgcgt aatgcctgaa aggctttgcc tgctggcgtg
      181 acaactcgct ttgatatcat atccctggca taaatcaatg gccgccgcga caccgttagc
      241 agagaaaaga gatgcaatca ttgacgggag tagagataaa tactccttgc gtagaaagat
      301 aatccgcata ttaatctgac catcatttta tataagacgg catatttaaa cgcattatac
      361 aatatgggtt atctttcttt tgtaaattgt ccaacccgat gcaatggcta ttttgttatt
      421 atttgatgaa taatatcagt gcgtcataat tcaagttaat aaccttaagg gataccagtt
      481 atatttaaac taaattatag taatgaataa ttttcttata atataaggta aattaacaaa
      541 atggcttcgc atttaacaat aaccgaatag aaaacaacca tttcgacatc aacaatcgct
      601 tacattcgct tatatattga ccacaactga tacatcagat tatgtgatga ctcgtgctta
      661 gatcaatttt tgcaatcatt aacaaaaaga ttaataaacc atctatatca atttatccaa
      721 cctattctgc cgttcaagaa atcgccgaac agttattttt aacaaatttt tctcttccca
      781 ttgactttcc cggacacctt gtctgaccta aggtgcgcga aagccacttt ttccttcctg
      841 agttatccac aaagttatgc acttgcaaga gggtcatttt cacactatct tgcagtgaat
      901 cccaaacata ccccctatat atagtgttct aagcagcttc ccttactaca ggtagtctgc
      961 atgaaactat tgcggaaaga attccaaaaa caggtacgac atacatgaat cagaatctgc
     1021 tggtgacaaa gcgcgacggt agcacagagc gcatcaatct cgacaaaatc catcgcgttc
     1081 tggattgggc ggcagaagga ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc
     1141 acattcagtt ttatgacggt atcaagacct ctgacatcca cgaaaccatt atcaaggctg
     1201 ccgcagacct gatctcccgt gatgcgccgg attatcagta tctcgccgcg cgcctggcga
     1261 tcttccacct gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg tacgaccacg
     1321 tggtgaaaat ggtcgagatg ggcaaatacg ataatcatct gctggaagac tacacggaag
     1381 aagagttcaa gcagatggac acgtttatcg atcacgaccg tgatatgacc ttctcttatg
     1441 ctgccgttaa gcagctggaa ggcaaatatc tggtacagaa ccgcgtgacc ggcgaaatct
     1501 atgagagcgc ccagttcctt tatattctgg ttgccgcgtg cctgttctcg aactacccgc
     1561 gtgaaacgcg cctgcaatat gtgaagcgtt tttacgacgc ggtttccaca tttaaaattt
     1621 cgctgccgac gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg
     1681 tactgatcga gtgcggtgac agcctggatt ccatcaacgc cacctccagc gcgattgtta
     1741 aatacgtttc ccagcgtgcc gggatcggca tcaacgccgg gcgtattcgt gcgctgggta
     1801 gcccgattcg cggtggtgaa gcgttccata ccggctgcat tccgttctac aaacatttcc
     1861 agacagcggt gaaatcctgc tctcagggcg gtgtgcgcgg cggtgcggca acgctgttct
     1921 acccgatgtg gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac cgtggtgtgg
     1981 aaggcaaccg cgtgcgtcat atggactacg gggtacaaat caacaaactg atgtataccc
     2041 gtctgctgaa aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg
     2101 acgcgttctt cgccgatcag gaagagtttg aacgtctgta taccaaatat gagaaagacg
     2161 acagcatccg caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac
     2221 gtgcgtctac cggtcgtatc tatattcaga acgttgacca ctgcaatacc catagcccgt
     2281 ttgatccggc catcgcgcca gtgcgtcagt ctaacctgtg cctggagata gccctgccga
     2341 ccaaaccgct gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg
     2401 ctttcaacct gggcgcaatt aataacctgg atgaactgga agagctggca attctggcgg
     2461 ttcgtgcact tgacgcgctg ctggattatc aggattaccc gatcccggcc gccaaacgtg
     2521 gagcgatggg tcgtcgtacg ctgggtattg gtgtgatcaa cttcgcttac tacctggcga
     2581 agcacggtaa acgctactcc gacggcagcg ccaacaacct gacgcataaa accttcgaag
     2641 ccattcagta ttacctgctg aaagcctcta atgagctggc gaaagagcaa ggcgcgtgcc
     2701 cgtggtttaa cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc tataagaaag
     2761 atctggatac catcgctaat gagccgctgc attacgactg ggaagcattg cgtgaatcaa
     2821 tcaaaacgca cggcctgcgt aactctacgc tttctgccct gatgccgtcc gagacctctt
     2881 cgcagatctc taacgccact aacggtattg aaccgccgcg cggttacgtc agcatcaaag
     2941 cgtcgaaaga cggtattttg cgccaggtgg tgccggacta cgagcacctg cacgacgcct
     3001 atgagctgct gtgggaaatg ccgggtaacg atggctacct gcaactggta ggtatcatgc
     3061 agaaatttat cgatcagtcg atctctgcta acaccaacta cgatccgtca cgtttcccgt
     3121 caggaaaagt gccgatgcag cagttgctga aagacctgct caccgcctac aaattcgggg
     3181 tcaaaacgct gtattatcag aacacccgtg acggcgctga agacgcgcag gacgatctgg
     3241 taccgtcaat ccaggacgat ggctgcgaaa gcggcgcatg taagatctga tattgagatg
     3301 ccggatgcgg cgtaaacgcc ttatccggcc tacggctcgg tttgtaggcc tgataagacg
     3361 cgccagcgtc gcatcaggct ccgggtgccg gatgcagcgt gaacgcctta tccggcctac
     3421 ggctcggatt tgtaggcctg ataagacgcg ccagcgtcgc atcaggcaca ggatgcggcg
     3481 taaaatgcct tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt
     3541 ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt
     3601 ggctcgctac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc
     3661 tttcttctgg cgtccggaag aagttgacgt ctcccgcgac cgtatagatt accaggcgct
     3721 gccggagcac gaaaaacaca tctttatcag caacctgaaa tatcagacgc tgctggattc
     3781 cattcagggt cgtagcccga acgtggcgct attgccgctt atttctattc cggaactgga
     3841 aacctgggtc gaaacctggg cgttctcaga aacgattcat tcccgttcct atactcatat
     3901 cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca
     3961 gatccagaaa cgtgcggaag ggatctccag ctattacgat gaactgatcg aaatgaccag
     4021 ctactggcac cttctgggcg aaggcaccca caccgttaac ggtaaaaccg taaccgttag
     4081 cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc
     4141 gatccgcttc tacgtcagct ttgcttgttc ctttgccttt gcagaacgcg aactgatgga
     4201 aggtaacgcc aagattattc gcctgattgc ccgcgatgaa gccctgcacc tgaccggtac
     4261 tcagcatatg ctgaatctgc tgcgcagcgg cgcggatgat ccggagatgg cggaaattgc
     4321 cgaagaatgt aagcaggagt gctatgacct gtttgtccag gcggcacagc aagagaaaga
     4381 ctgggcggat tatctgttcc gtgacggttc gatgattggt ctgaataaag atattctctg
     4441 ccagtacgtt gaatacatca ccaatatccg catgcaggca gtcggtttgg atctgccgtt
     4501 ccagacgcgc tccaacccga tcccgtggat caacacctgg ctggtgtctg ataacgtgca
     4561 ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt
     4621 ggacaccgac gatttgagta acttccagct ctgatggccc gcgttaccct gcgcatcact
     4681 ggcacacaac tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac
     4741 aatgttgcgg ttgagtacca gtgccgcgaa ggttactgcg gctcctgtcg cacccgactg
     4801 gtcgcaggtc aggtggactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt
     4861 ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg agatgtgaat tggttgtagt
     4921 gccagataca acgcttatac gtcttatctg gcctacaacg attacatggc gtagtaatac
     4981 gctttcacct gctcccagtc cgctttgggg attggctcca gatattttcc agctggcgga
     5041 agtcatgatt aatcgcttta ttgcgacgca agcggcgacg acttttctcc aggtcaagaa
     5101 aaccagcttc tgctttacct tctgttttca catagatatg gcgaacataa caacagccat
     5161 gctgacgatt aacgctatgc attttcttaa acgccagcgc cactgctttc aacatggctt
     5221 gccgtacttc gtcagaatag ggcgatactg catgccgggc ataccagtca gcaatgctga
     5281 tgaaccccgc catatcttca gtcaccagca acgcgcgcca ttcaccctca attttcaccg
     5341 cttcaccaaa aacgatcttc ggcacgatga caccggcccg ttccagttct ttaattaccg
     5401 caacctcacg gacaatcgtt ggtcggccga acggataacg tacggaatga aacagatgat
     5461 gcgtcatgcg ctttacatac agctttttgc cgttgcgctc gacgcattgc accccgctca
     5521 taccattacg gcgatagtta ggctcttcaa cccagtcgcc ctctgttgcc caccagtgat
     5581 taaattcgtc gtactttgct gaaactgcca taccctatcg cctgtcattt ttattaacga
     5641 caatgactat aggtggttac ctgaggaaaa tcttaatgaa acgtgtcgta ttaatgaatt
     5701 tcttttgccg ctactagtgt gacaccgctt ttgcccttca tttgcagcgc atagattcca
     5761 ccagccacga ccactaatag tgcgcccagc agataaaatg tgaatttaat caggtggata
     5821 cagcgatgaa ttttattcga cagggattag gcatcgcctt attaccagag ttaacgctga
     5881 aaaccattgc aggtgaattg tgttccgttc ctcacgaacc aaccttctat cga
//
LOCUS       DAJTXQ010000192         5919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000192 DAJTXQ010000000
VERSION     DAJTXQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.193"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            60..410
                     /gene="traA"
                     /locus_tag="OO515_003879"
                     /pseudo
     CDS             60..410
                     /gene="traA"
                     /locus_tag="OO515_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            425..736
                     /gene="traL"
                     /locus_tag="OO515_003880"
     CDS             425..736
                     /gene="traL"
                     /locus_tag="OO515_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCR6577686.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            758..940
                     /locus_tag="OO515_003881"
                     /pseudo
     CDS             758..940
                     /locus_tag="OO515_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            942..2192
                     /locus_tag="OO515_003882"
                     /pseudo
     CDS             942..2192
                     /locus_tag="OO515_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2189..2575
                     /gene="trbI"
                     /locus_tag="OO515_003883"
     CDS             2189..2575
                     /gene="trbI"
                     /locus_tag="OO515_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCR6577687.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2572..2883
                     /locus_tag="OO515_003884"
                     /pseudo
     CDS             2572..2883
                     /locus_tag="OO515_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            2878..3876
                     /locus_tag="OO515_003885"
                     /pseudo
     CDS             2878..3876
                     /locus_tag="OO515_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3896..4643
                     /gene="traX"
                     /locus_tag="OO515_003886"
                     /pseudo
     CDS             3896..4643
                     /gene="traX"
                     /locus_tag="OO515_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4698..5258
                     /locus_tag="OO515_003887"
     CDS             4698..5258
                     /locus_tag="OO515_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6577688.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5388..5600
                     /locus_tag="OO515_003888"
     CDS             5388..5600
                     /locus_tag="OO515_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6577689.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1396 a         1437 c         1805 g         1281 t
ORIGIN      
        1 atctggcagc ctgagttcac agataaaaca ctctccagga aacccggggc ggttcagcgt
       61 taagtgttca gggtacttct gcgcccgtca aaaagaaatc gtttttttct aaattcactc
      121 gtctgaatat gcttcgcctg gttcgtgcag tgatcccggt tgctgttctg atgatgttgt
      181 tccctgagtt agctatggct gctggtaaag gcacggacct gatggctaaa ggtcaggaaa
      241 cggttaaggc gaccttcggt aaggactcca gtatcgttaa atgggttgtt ctggctgaag
      301 ttctggtcgg tgcagtcatg tacatgatga ccaaaaacgt caagttcctg gtcggctttg
      361 ccatactctc tgtatttatt gcagtaggta tgtctgttgc cggattctga caggaaataa
      421 aacgatgttg ggagacgaga ataaacttaa gaaatatcgt ttcccggaaa cactgaccaa
      481 ccagagccgc tggtttggcc tgccactgga tgaactgatc cccgcagcaa tctgtgttgg
      541 ctggggtatc acaacatcga aatatctgtt cggtattggt gcagcggttc tggtttattt
      601 cgggattaaa aaactgaaaa aagggcgggg cagttcctgg ttacgtgacc tgatttactg
      661 gtatatgcca acagccctgc tgcgcggtat ttttcataat gttcccgatt cgtgtttccg
      721 gcagtggatt aaatagaact gataccagga ttgttatatg gaacacggtg cccgtttaag
      781 taccagtcgt gtaatggcca tcgcctttat atttatgtca gtgcttattg ttctcagcct
      841 ctctgttaac ctcattcagg gggtgaataa ctaccgtctt cagaatgagc aacgcactgc
      901 cgtgacccca atgggattta atgtgccctt tgccgtgtca cacctaccgt aaccagctgg
      961 cgtttatcga tattttcttc cggggaatga ataacaccaa ctacaacatg gcggtctgtg
     1021 gcacctccgg ggccggtaaa accgggctga tacagccact tatccgcagc gtgctggact
     1081 ccgggggctt tgccgtggtg ttcgacatgg gggatggcta caagtccctg tgtgagaaca
     1141 tgggtggcgt atatctggac ggtgaaaccc tgcgttttaa cccgtttgcg aacatcaccg
     1201 atatcgacca gtcagcggag cgtgtccgtg accagttgtc agtgatggcc agccccaacg
     1261 gtaacctgga tgaagtgcat gaaggtctgc tgctgcaggc ggtcagggcc tcctggctgg
     1321 ccaaagagaa cagggcacgt attgatgacg tggtggattt cctgaaaaac gccagtgaca
     1381 gcgagcagta tgccgggtca ccgactatcc gcagccgtct ggacgaaatg attgtgctgc
     1441 ttgaccagta cactgccaac ggcacttacg gccagtattt taactctgat gagccgtccc
     1501 tgcgggatga cgcaaaaatg gtggtgctgg agctgggcgg actggaagac cgtccgtcac
     1561 tgctggttgc ggtgatgttc tccctgatta tctacatcga gaacaggatg taccgcacgc
     1621 cgcgtaacct caagaaactg aacgtcattg atgaaggctg gcgtctgctg gacttcaaaa
     1681 atcacaaggt cggtgagttt attgagaaag gttaccgtac cgcccgtcgt cataccggtg
     1741 cttatatcac catcacacag aacatcgtcg actttgactc tgacaaggcc tccagtgcgg
     1801 cccgtgcggc atggggtaac tcctcctaca aaattatcct caaacagagt gcgaaggagt
     1861 tcgcaaaata caaccagctg tatccggacc agttccagcc actgcagcgc gacatgattg
     1921 gtaagtttgg tgcggccaaa gaccagtggt tcagttcctt cctgctgcag gtggaaaacc
     1981 attcctcctg gcaccgcctg tttgtggacc cgttaagccg cgccatgtac agctctgacg
     2041 gcccggattt tgagtttgtg cagcagaaac gtaaagaggg gctgagtatt catgaggcgg
     2101 tgtggcagct ggcgtggaag aagtcagggc cggagatggc ttcgctggaa gcctggctgg
     2161 aagaacatga gaaatacagg agtgttgcat gaccacaacg caaaaaacga ctgatgttac
     2221 agccccccga cgcagccact ggtggtggac ggtgccggga tgcctggcaa tggtgttact
     2281 gaacgcagcc gtcagttacg gcattgtcag actgaacgca ccgatgactg ccgccttcaa
     2341 catgaaacag actgtggatg cattttttga cagcgccagt cagaaacaac tgtcagaagc
     2401 acagtcaaaa gccctttcag cgcgttttaa cacggcactg gaagccagcc tgcaggcgtg
     2461 gcagcagaaa caccacgcgg tcattctggt gtcgcctgcc gtggtacagg gggctccgga
     2521 tatcacccgg gaaatccagc aggatattgc ccggcgaatg agagcggaac catgagatgc
     2581 cgggggctga ttgccctgct gatatggggg cagagtgtgg ccgccgccga tcttggcacc
     2641 tggggtgatc tgtggccggt aaaggagccg gacatgctga cggtgatcat gcagcgcctg
     2701 acggcactgg agcagtccgg tgagatgggc cggaaaatgg atgcctttaa ggagcgggtg
     2761 atccgcaaca gtctgcggcc ccctgccgtg cccggtatcg gacgcacgga gaaatacagc
     2821 agccggttgt ttgacccgtc cgtcagactg gctgcggata tccgggataa cgaagggcgg
     2881 gtggccggct ttgagtcagc ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg
     2941 tacaccgata accgtcaggg ctggacggat gccatcaaca atgccgtaca gaaaggaact
     3001 gcccacgatg tgtttgagcc gaaaccggac cgggaggtca tgaatgcaga gcggctgttc
     3061 agtacggcgc gggagctgcg ggacgtggcg gcaggccgtg ccgttctccg tcaggcgggg
     3121 ctggccgggg gagacagtcc tgcacggttt attgctccgg gacgtaaata tccgcaaccg
     3181 tatgtggcac tgccggcgtt tgaccgtaac ggcaagtccg ccggtatctg gctgaacccg
     3241 ctgaccacgg atgacggaaa cgggctgcgg ggattcagcg gtgaaggtcg ggtaaaaggc
     3301 agcggggatg cgcagttcgt ggccctgcag ggcagccgta acggagaaag cctgctggct
     3361 gataatatgc aggatggcgt ccggattgcc cgtgataatc ctgacagtgg tgtggtggta
     3421 agcattgccg gtgaaggccg tccgtggaat cccggtgcca tcaccgatgg ccgcgtgtgg
     3481 ggggatatcc cggacaacag cgtccagccg ggagccggaa atggcgaacc ggtcacggca
     3541 gaggtactgg cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgtcgcgca
     3601 gatgaaattg tccgtaaaat ggcagagaac aaacctgacc tgccggacgg caaaacagag
     3661 ctggctgtca gggaaattgc cgggcaggag cgtgaccggg ctgccataac tgaacgggaa
     3721 gccgcgctgc cggagagtgt gctgcgtgaa tcacaacggg agcgggaagc ggtccgggag
     3781 gttgcccggg aaaatctgct gcaggagcgt ctgcagcaga tggagcggga tatggttcgt
     3841 gacctgcaga aagagaaaac cccgggcgga gactgataca ggaagacaaa cactgatgac
     3901 aacggataac acgaacacga cacgtaacga ttcactggct gcacggaccg atacctggtt
     3961 gcagtcattt ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt
     4021 gctgatggta ctggaccaca tcaatctgat attccagctg aagcaggaat ggatgtttct
     4081 ggccgggcga ggagcctttc cgctttttgc cctggtgtgg gggctgaatc tgtcccgtca
     4141 tgcgcatatc cggcaaccgg ccattaaccg gctgtgggga tggggaatta ttgcccagtt
     4201 cgcatattat ctggcaggct ttccctggta tgaggggaat atcctgtttg cctttgcagt
     4261 ggcggcacag gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat
     4321 tcttctgatg gccctgttgg gggcctttgt ccggcaccag ttacggcatt gccgggctgc
     4381 tgatgctggc agtcagccac cgactgtacc gggcggaaga cagagcggaa cgtctggcgc
     4441 tggtcgcctg cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg
     4501 cggtggccgg tcttgtgatg acggtgctga ccgttggtct ggtgtcgtgt gcaggaaaat
     4561 cattaccccg tttctggccc ggggattttt tcccggtatt ctacgcctgt catctggctg
     4621 tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg accgtaacag gatgattcgt
     4681 cagaggcaga acacagcatg acagagcaga agcgaccggt actgacactg aagcggaaaa
     4741 cggaagggga gacgcctgtc cgcagccgga aaaccatcat caatgtcacc acgccaccaa
     4801 agtggaaggt gaaaaagcag aagctggccg agaaagcagc ccgggaagca gagctggcgg
     4861 caaaaaaagc gcaggccaga caggcactgt ccatttatct gaacctgccc acgctggatg
     4921 aggccgtgaa taccctgaaa ccctggtggc cgggattatt tgacggtgac accccccggc
     4981 ttctggcctg cggtatccgg gacgtgttac tggaagacgt ggcgcagcgg aatatcccgc
     5041 tctcgcataa aaaactgcgc agggcgctga aggccatcac ccgttcagaa agctatctgt
     5101 gtgccatgaa agccggtgcc tgccggtatg acacggaagg gtatgtgacg gagcatattt
     5161 ctcaggagga agaagcgtat gcggcagcgc gtctggataa aatccgccgc cagaaccgga
     5221 taaaggcaga acttcaggcc gtgcttgatg agaaataaaa aaaagcctcc atcagagagg
     5281 aggcagggaa ataaggctta aaaggaaata acttccgctt ccgaaaggaa gccagtccac
     5341 tgaattattt atcacaataa tactgacaac taaaggggga caagaaaatg aatggattca
     5401 gaaacagttc acggaacggc caggtctggc gttaccagcg tgccggcggg cgggccgtta
     5461 ttctggaagt cagtggacgc tggatggaag cggcagaagc atggcgacgg gctgcctgtg
     5521 ttgcgcccag gacagactgg cagcagtttg cccgaaaaag ggctgaacac tgccaccggc
     5581 gctgccgggg aagggtgtaa cgaaaaaagc agccttcatt actccaggag tcagaaggct
     5641 gaaaggagtt tatgagattt gcttttttta atatccggtc gggcagtatg accggacagt
     5701 tctgagtagc acagagtttt ttccgtatta agtcgtgatt ttatattttg tgattaattt
     5761 cacaaaataa ggtgttattc agtgtgtgct gcaatattca ggatgcctga aatacagatg
     5821 ctatttatct gatatggaga ataacatgag gaaatagtaa ggcagtatga ctttccgtga
     5881 gacagatgct ggccaacgtg ggatatttaa cgcgggtta
//
LOCUS       DAJTXQ010000193         5837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000193 DAJTXQ010000000
VERSION     DAJTXQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.194"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO515_003889"
     CDS             complement(<1..242)
                     /locus_tag="OO515_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6577690.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OO515_003890"
     CDS             complement(604..3387)
                     /locus_tag="OO515_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6577691.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OO515_003891"
     CDS             complement(3444..5816)
                     /locus_tag="OO515_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6577692.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1487 a         1321 c         1312 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttgcgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacagc
//
LOCUS       DAJTXQ010000194         5832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000194 DAJTXQ010000000
VERSION     DAJTXQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.195"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO515_003892"
     CDS             complement(139..3654)
                     /locus_tag="OO515_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6577693.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..4408)
                     /locus_tag="OO515_003893"
                     /pseudo
     CDS             complement(4106..4408)
                     /locus_tag="OO515_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(4682..5701)
                     /locus_tag="OO515_003894"
     CDS             complement(4682..5701)
                     /locus_tag="OO515_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6577694.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         1443 a         1524 c         1222 g         1643 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acagtattta tatgaacgca tcccgtttgc aaggcatcca
     4441 ttgttttgac atgacgggta ggttgcagct ttatatccgg ctttattgca gccgtaacgc
     4501 tgcgagcccc tatgtaattc gccgactcac cccttatttt cacggcggac ttcaagccca
     4561 aaatactgtt caggtttagt gagtcacggt cttacctgtc tttgtcatca ctctggccgc
     4621 catagcaacc tttcagcatt cacccttttg ttaagagttc attgtttaat ggtatcagcg
     4681 attaatatgg tctgatactg acgtggttcc tgtcatactt acggtcatgg gcggttatcg
     4741 cccatactgt tcgtgccagc ttgtttgcca tggcgacgat tgccacactg gctggacgac
     4801 gttttttcag ttcggttatc cacgggccag gctctttagc tactaatgcc accgctcttg
     4861 caccgtggat aaataatgtc ctgagataag tgtcaccacg tttgcttatc cccagcagac
     4921 gtactttccc tccggagcca gtttgttttg gaaccagacc aacatatgcg gcgaactctc
     4981 gccccgattt aaacgctgat gcttccccca tggtggccac cgctgccgtc gcaataagtg
     5041 gtccaacgcc aggaatatcc agcaaccgct tacaggtttc attctgcctc gccacgctgg
     5101 taagctgttt ttcaatatcc tctatcagtg agtccagctc attcagtcgg ttgtactggt
     5161 tttccagaac cataatgaga tacggtggca gtctctcttt catccgttcc agtgcttcgg
     5221 ggaactcccg ctccatcgct gcccggcctt tgtggatggt ttcaccaaac tccagtaacg
     5281 tcccgtgcag ggcattaatt tgtgcggtcc ggaacttcac cagttgcatg cgggtacggt
     5341 gcagaaccag taccgactgc tgttcttctg tttttacggc gatttcttta cccggctgct
     5401 gaaccgccat ccagatagcc cgggcatcca tcacatcatt cttattgccc atgacgaatg
     5461 ccttaacgaa gcgggcctgc aacaaccgga ctttatgacc aagttttgtc agttcccgtg
     5521 cccagtgctg agaacctcca caggcctcca taccaatcag gcatggctca cggttgccga
     5581 agaacgtcag aaaatcctgt ctacgcaact gtttatcaac cacttcacct gtgtgctcat
     5641 tgatgaagtg aatctgaatg acatgttttg cgatatcaac gccaaccggt gtatatttca
     5701 tttgtggagt ctccagtctg agggagcttt atgcatcccg tattgggcac tatgatgccg
     5761 gaaatctgcg aggctccaca cctgccagtt tcaccctttt aaccatcata ctgctccgtg
     5821 tttaattgtg tg
//
LOCUS       DAJTXQ010000195         5781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000195 DAJTXQ010000000
VERSION     DAJTXQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.196"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..747
                     /gene="mdtL"
                     /locus_tag="OO515_003895"
     CDS             <1..747
                     /gene="mdtL"
                     /locus_tag="OO515_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6577695.1"
                     /translation="PVLGHLIMLKFPWQSLFWAMAMMGIAVLMLSLFILKETRPASPA
                     ASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEYATIMA
                     LTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICA
                     GFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGIL
                     IACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            722..1681
                     /gene="yidZ"
                     /locus_tag="OO515_003896"
     CDS             722..1681
                     /gene="yidZ"
                     /locus_tag="OO515_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6577696.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            1838..2587
                     /locus_tag="OO515_003897"
     CDS             1838..2587
                     /locus_tag="OO515_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6577697.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            2609..3159
                     /gene="yieF"
                     /locus_tag="OO515_003898"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             2609..3159
                     /gene="yieF"
                     /locus_tag="OO515_003898"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3213..4550)
                     /gene="adeP"
                     /locus_tag="OO515_003899"
     CDS             complement(3213..4550)
                     /gene="adeP"
                     /locus_tag="OO515_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6577698.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            4716..5381
                     /gene="yieH"
                     /locus_tag="OO515_003900"
     CDS             4716..5381
                     /gene="yieH"
                     /locus_tag="OO515_003900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6577699.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            5448..>5781
                     /locus_tag="OO515_003901"
     CDS             5448..>5781
                     /locus_tag="OO515_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577700.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSE"
BASE COUNT         1460 a         1461 c         1463 g         1397 t
ORIGIN      
        1 ccagtgctcg gacatctgat tatgcttaaa ttcccgtggc agagtctgtt ctgggcgatg
       61 gcaatgatgg gcatcgcggt actgatgttg tctttgttta ttttaaaaga aacgcgccca
      121 gcgtcccccg ctgcttcgga caaaccacga gaaaatagcg agtcgctgct taatcggttt
      181 ttcctcagcc gtgttgttat caccaccctc agcgtttcgg tgatcctcac tttcgtcaat
      241 acatcgccgg tattgctgat ggaaatcatg ggttttgagc gcggagaata cgccaccatt
      301 atggcgttga ccgctggcgt cagcatgacc gtttcattct ccacgccatt tgcgctggga
      361 atttttaagc cacgtacgtt gatgatcacc tcgcaggtgt tattccttgc ggcggggatc
      421 acccttgccg tttcaccttc ccatgcggtt tctctgtttg gtatcacgct gatttgcgcc
      481 ggtttctcgg taggttttgg cgtagcgatg agtcaggcgt taggaccatt ttcattacgc
      541 gcgggcgtag ccagctcgac cttaggtatt gcgcaggttt gcggttcgtc actgtggatt
      601 tggctggcag cggtggttgg tattggcgca tggaatatgc tgatcgggat tctgattgcc
      661 tgtagcatag tgagcctgtt gctgattatg ttcgtcgcgc ctggacgccc cgttgccgct
      721 catgaagaaa tccatcacca cgcttgatct caatctgctg ctctgtctgc aactgctgat
      781 gcaggagcgc agcgtgacca aagcggcgaa gcggatgaac gtgacccctt cagcggtgag
      841 taagtcgctg gcaaagttaa gagcgtggtt tgacgacccg ctctttgtga actcaccgct
      901 gggcctgtcg cccacaccgc taatggtcag catggagcaa aatctggcgg aatggatgca
      961 aatgagcaat ctgctgctgg ataaaccgca ccaccagaca ccgcgcggcc tgaagtttga
     1021 gttggcggcg gaatcgccgc tgatgatgat catgcttaat gcgctgtcga aacggatcta
     1081 ccaacgttac ccgcaggcga ccatcaaact acgtaactgg gattacgatt ccttagatgc
     1141 cattactcgt ggtgaagtgg atattggttt ttccggtcgc gaaagccatc ctcgctcgcg
     1201 ggagctgtta agctcgctac cgttagccat tgattatgaa gtgctgttta gtgatgtacc
     1261 ctgcgtctgg ttacgcaaag atcatccggc actgcatgaa acgtggaatc tggacacctt
     1321 tttacgttat ccgcatatca gcatttgctg ggaacagagc gacacctggg cgctggacaa
     1381 tgtgttacag gagctggggc gcgaacgcac gattgctatg agtctgccgg aattcgagca
     1441 gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc cgcgctactg
     1501 tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc cgtttgacga
     1561 aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac ggaacagcca
     1621 taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt cgatggcata
     1681 accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt tgtaacaagc
     1741 ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa atgattactc
     1801 ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact ttgcccgagg
     1861 gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc atcaagaagc
     1921 ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt tactcgcaga
     1981 actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc tgccgaaagg
     2041 taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg ccgggaatat
     2101 ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac tacagcgtgc
     2161 gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca gcaatgaatc
     2221 gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca tcacgctgcg
     2281 ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg atctgcagct
     2341 gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag cgttatgcga
     2401 cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa agctcagtgt
     2461 ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca gccgcgccaa
     2521 caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt ctttttcgcc
     2581 aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg ttacgttact
     2641 ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc cgaaaattgc
     2701 tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc tgtatgacgc
     2761 tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg aacagatccg
     2821 tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactctgtac cgggtgggct
     2881 gaaaaatgcc atcgactggc tttcccgctg gccggtaaac cggtgttgat tcagaccagc
     2941 tcaatgggcg tgattggcgg cgcgcgctgt cagtatcacc tgcgccagat tctggttttc
     3001 ctcgatgcaa tggtgatgaa caagccggaa tttatgggcg gcgtgattca gaacaaagtt
     3061 gatcggcaaa ccggagaagt gattgatcag ggtacgctgg accacctgac cgggcaattg
     3121 accgcatttg gtgagtttat tcagcgagtt aagatctaaa taaaaaaccc gccagtaatc
     3181 atgcatggcg ggtttttaat gcgctatcga ttttaatgag cgtcgataaa tacaatcttc
     3241 aggataaaca gcagcgcaac gatgattacg cacgggctaa ggtcacgcag acgcccggta
     3301 ccaatcttca tcacgcagta ggagataaag cccagcgcaa taccttcggt aatcgaaaag
     3361 ctgaacggca tcatcacggc ggtaataaac gccggaacag attcagtaag atcctgccag
     3421 ttcacgcgag caagacttga ggtcatcaac acgccaacgt agatcaacgc gccagctgca
     3481 gcgtagcctg gcaccattcc cgccagcggc gacagaaaga taaccagcag gaacaacaga
     3541 ccaacaacca ctgccgtcag accggtacga ccgccaaccg atacgccgga agaggactca
     3601 atataagccg taacggaaga agtaccgata aacgaaccgg tcacggaaga gatactgtcg
     3661 acatacagcg cctgcttcat gcgcgggaat ttccccttct catccgccag acctgcttta
     3721 tcggtcacgc caatcagcgt accggaggag tcaaacaagt tgaccaacat gaaagagaaa
     3781 atcacccctg ccagcccgag gttaaacgac ccggctaaat ctacatgacc cacaactgtc
     3841 attacgctcg gcggcgcaga aacgatgcca ttgtagtgca catcacccag catccagccc
     3901 agcagcgtcg tcaccacgat agaaaccagc accgctgcgt gaatgttgcg cgaggccaga
     3961 atggcgatga tgaagaaacc gaggatcccc agcagcacgc tgtgagaagt cagattaccg
     4021 atgctcacca gcgtttccgg gttagcgaca atcacacctg cgtttttcag ccccatcatg
     4081 ccaatgaaca gaccgatacc gctggtaata cccacacgca gacttaccgg aatgttagca
     4141 atcatccagt aacgaacgcg gaaaatggtc aaaagaagca gtccgacagc gccccagaag
     4201 attgcgccca taccgatctg ccacggcagc cccattgcct ggacaaccac aaaagcgaaa
     4261 aaggcgttaa gccccatcgc aggagccagc gccaccggaa ggtttgcaaa caatcccatc
     4321 ataatgctac cgaaagcagc aatcagacag gttgttacga agacggcact ggtgtccatg
     4381 ccagcaacgc ccagaatttg tgggttaaca aatacgatgt agaccatcgt caggaaggtg
     4441 gtgaacccgg caatcacttc agttcgtgcc gtagtaccat gttcgcgcag tttaaaaact
     4501 cgttccagca tcccctgacc agaagtctgg gtggtatgtt gatgactcat tatctatttc
     4561 cgaacaagga gggaaaatcc gtcgctatcg tataccaaaa tgcgacaata ggcgcgtttg
     4621 tgagagactt tttttattgg atttacttat acggcaacga ttgcgttgcg caaatcggct
     4681 ctacgaaaac gttaaactga ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt
     4741 tcgactgcga cggtacgctg gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga
     4801 tgtttcagga atttggtatt acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg
     4861 taaaactgta cgaaattatc gatattgttt cccttgaaca tggtgttacg ttagcgaaaa
     4921 cagaatctga acacgtttac cgtgcagaag tcgctcggct gttcgattca gaactggaag
     4981 ccatcgaagg ggctgaagcg ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta
     5041 acggcccaaa taacaaaatg cagcattcta tgggcaagct gaatatgttg cactacttcc
     5101 cggataaact gttcagcggc tacgatattc agcgctggaa gccagacccg gcgttaatgt
     5161 tccatgcggc aaaagcgatg aatgtaaatg tagaaaactg cattctggtt gatgactcag
     5221 ttgccggtgc gcaatctggt atcgacgcag gtatggaagt gttctacttc tgcgccgacc
     5281 cgcacaataa gccgatcgtt cacccgaaag tcaccacctt tacccatctt tcgcagttac
     5341 cggaactgtg gaaagcgcgt ggttgggata ttacggcata gttcttcaca ctcccttcac
     5401 ttaccccgct taaattggcg ctcaaaggta agtaaaggga gtttgatatg tctgtttcac
     5461 gtcgggtaat acatcacgga ctttattttg cggttttagg accgttaatt ggtgttctgt
     5521 ttcttgtcct ctacatattc ttcgcaaaag aaccgctggt tcttttggta ataatacagg
     5581 tacttccatt gtttctctta ttgtcgatta ctacgggagc tattcctgca ttgttaaccg
     5641 gcgtgatggt cgcctgtctg ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg
     5701 ctggtggcat aggtggcgtc gttatcaccg agatctattg tgcagttatt gtacatatta
     5761 agggcatggc ttcctcggag t
//
LOCUS       DAJTXQ010000196         5720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000196 DAJTXQ010000000
VERSION     DAJTXQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.197"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            78..154
                     /locus_tag="OO515_003902"
     tRNA            78..154
                     /locus_tag="OO515_003902"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:112..114,aa:Arg,seq:tct)
     gene            complement(199..960)
                     /gene="envY"
                     /locus_tag="OO515_003903"
     CDS             complement(199..960)
                     /gene="envY"
                     /locus_tag="OO515_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6577701.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1143..2033)
                     /locus_tag="OO515_003904"
     CDS             complement(1143..2033)
                     /locus_tag="OO515_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6577702.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2034..5006)
                     /gene="nfrA"
                     /locus_tag="OO515_003905"
     CDS             complement(2034..5006)
                     /gene="nfrA"
                     /locus_tag="OO515_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6577703.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4993..>5720)
                     /gene="nfrB"
                     /locus_tag="OO515_003906"
     CDS             complement(4993..>5720)
                     /gene="nfrB"
                     /locus_tag="OO515_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HCR6577704.1"
                     /translation="LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESI
                     DAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVR
                     YFIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFA
                     EILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQ
                     SLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1290 a         1520 c         1500 g         1410 t
ORIGIN      
        1 caaccagatc aaaagccatt gactcagcaa gggttgaccg tataattcac gcgattacac
       61 cgcattgcgg tatcaacgcg cccttagctc agttggatag agcaacgacc ttctaagtcg
      121 tgggccgcag gttcgaatcc tgcagggcgc gccatttctt cttcatttaa ccccgtctta
      181 tccgttccgc tttgctcttc accacatcac tttttgtcgc tgtttggcga gataattcaa
      241 cggtgtcagg ccgtaaaacg ccttaaaaac agagataaag tacgacgtgc tgctatagcc
      301 acataattgc gccacctgag tgatattttt gttatccatc aataacatct gtacggcgta
      361 acgcatacga cactctgtga caatctggct atagctgata ttttcgtttt ttaatttctt
      421 tttgagcagg ctggggctta aacatagcga actggcgaca attcgcagat tccagtaatg
      481 ctgaatatcg ctttgaataa tgcggcagac gctgtcgcgg acgctgctgc gtaagatata
      541 catcagtagg gcaataaacc gcgattgctc aagaaagtta gacaatacgg taaaaagcaa
      601 tgcgcgcgtc aactccgctt cgcagggttt gctgggttgc tgcttgctgt ggttggcggc
      661 tagccggaac acttccggtg tacggctatg acaagcgata accggggttg ccagccgcga
      721 ccagggcttt acacaggtta agttcttatt taaaaacaac agataatcgt tgatgatgtt
      781 acggttgatg tgggtgatta atacgtgatt taacgttgag agttcaatga cgttattgtt
      841 gcacgcgaag gcggccaggt agtttttcgg aagggtaaac gtagcatggt tattgacgct
      901 tacctctacc tctttttcgg tcaggatcac cacgcaaggt tcactgctgc tcaattgcat
      961 ttcgcactcc tcagatatca gaaactccgc tcaaaggatc tatgcttcct gcatgagtga
     1021 tcggcccgtt cgccgataac gatcttcttt ctttagcacg ctttttagca attaatcttg
     1081 atggaattct gacgagagcg aaagaggtaa gccaggtcgt acccgactta cctggaggag
     1141 atttaatact cgagaatgcc gtgcgcgacg ggcaaatagc gcagagagaa atagagagtg
     1201 tctttaccgc aagaggaacg tttcgctaac agcgaggcga tctctgcatc cggtttcggt
     1261 ttcgctgtaa aggttttgcc tttgccggcg acaaacagtt cataaacaat gccgcttgct
     1321 ggggcggagc tttggcaatc ggcgctggcc tgtagataac gttcacgctg ttcagcggta
     1381 agtttatcca cgccgctgcc atcctgtacc cagacattaa ctccggttgc tttaacttgt
     1441 tccagcagtt ggcgatagcc atctggcgac atgtttccgg cgaaaaaact actgatataa
     1501 accggttttg ctgaaacatc gctaatcagc cgctgcgcgt tgtttaacca tgttagcaaa
     1561 ggctgacggg cagcttcgct gcgccagttc aggtcgtcaa tttccgcgct gatgtaccag
     1621 ccatccggcg ttatgccagg cgcggcgctc cataatctgg cttgctggag atcggcagcc
     1681 agcaggcgat taagatagct ttccagcgct gcggacgact gtttctggtg cataaaaaat
     1741 tccggatcgg cgttcagccc gacaatcagt ttcaggccag cctgttgcgc agctgcggca
     1801 cgcttaaaca ataacgtgcg ctgttctggc tgggtaaatg catcgccgta acgggtccat
     1861 tgcaaaacca gggtatcgaa gccttgcaaa cgtaactgac tcatcagccc ctgccactgg
     1921 gtatcggtaa cctgactatc tcggttttgt ggttgccaga taataccttt catcgcaaag
     1981 gaaaaagggc tgaccaaaag cagtgtcagc aatacgaaaa tgaacttacg catttaccag
     2041 tgcactccaa tggtgagaaa cgcgttgttg cgctctccgt tacgttgatt aatcgcctta
     2101 aaggtatgtt gatactcgac gccgagactg actttgtgcg gccaggcgtc gtagtgcgtc
     2161 tcgccggtcc agatattcca gcggaccccg actccgccaa gctgcgcgcc ctgagtgcct
     2221 ttatcacgat agccgttgtc ctgaacgtga gcgtaaggct caatagtctg tccgttagct
     2281 accttctgat gccagctgac gcgataatct gccgtccacg cctgaatatc ctggcggata
     2341 tattgcgccg catcgaggta caggttttgg gcaaaccagc ctgaaccgtt cgggtgccat
     2401 tcgtcgctgt atttgccgcc attaaagaat gaggcgctgg cgcgcagcat ggtatcggat
     2461 gcgccatttt gcccgttcag cggcaactgc tgttcgacgg cgaggaaaaa gatctgatcg
     2521 cgcagcggct tccagcgcag accggtgccg gacatcggat ttttcaccgg catcatcacc
     2581 ccgttttctc cggtatcggc aaagacgcgg ctataaactg agagcaggtc gccttccagc
     2641 agcatattgc gtccgatgcg gtactcggct tccagttgcc cgtagctacg atagcttttc
     2701 cctggcgctg cgccgccaac attattgtta gcggtactca ttgccccgga acgcaagccg
     2761 atagaagaat cgaaactgaa cgtccagcgg cgaccgacct cctcatgcaa acggcggaaa
     2821 ttgaagcgtt gttgattttg ctctggggtc agtggggtta tcagagcctg attatcaatg
     2881 tcatcaatca ccagccgggc gtagtgctgc gtcgcaggca tgtcatccag acgctggttc
     2941 acgtaggcca gttgtcggat cagtgccgga tcgtccggaa gccttttatg cgcctgttcg
     3001 agcatttccc gcgactgtgc gatatcaccg ctatcccaca aggcgtaacc aagcgctgcc
     3061 tggatgttgc tattattcgg ttccagttcc agcgcggcgc gcaaatcact caccgcggcc
     3121 gggacattat gacgttggcg ataaattgtc gcccgcgcaa cgtaagcgtt ggcagaaggc
     3181 gcaatattga ttgagcgcgt gagatcgttc agtgcgagtt ccggctgacc aggaatgtaa
     3241 cgttgtgcat gcagccacca gtagagggca ttgcttccca gtccacgttg ttctgcctgt
     3301 tgtagccagc gatcgcgagc cgcaccattt cctgccgcct gggcggtatt ggcagcagca
     3361 agcagatcct cattgctcat gtcgtgaaga ctgattttct gccaggccgc cagtgcggtg
     3421 gcgtagtcct caacctgata cgcctgatag gctaccgcac gatgttgcca ggcgctcggt
     3481 tgtcgttgtt cggcctgaag ccatgcatac aacgccacac cgggtagcgt gtcccgataa
     3541 cactttgcca gacggttcca ggcggcagca tcgtaggaag gcgacatatc gcccagcaag
     3601 cgaactattg ccgggcaatt atctgcaata cccggcaact gactttgcca ctgacgttgc
     3661 tccgccagcg gtaagggttt cgataaaatc gccaccttcg ccggcgttgc caggtaagga
     3721 tgactttcca gcagagacgc cagtcgcgcc attaaagtct ggctgacacg cgcatcgccc
     3781 tggaaaggat agcgttgcag caataaatcg gcagcttcgc gtgactgctc gttctgcatc
     3841 agttgccagg tcagttgatc caggcgggta agatttgccg gttcttgctg atacagcaat
     3901 cgtgccagac gcagagattc agccttgtta cgggtcgcca cgctgacagc ataacgctcc
     3961 tcaagcattt cattggcggg gagggtggcg agcagttttt gcgctgcgtc gtactgacct
     4021 tcttttaaca gcaccggtag cgtcgcgcca acaacatact ggcggttatc ggcaaactgc
     4081 accgtataat tcgccaatgc ctgaacgggg ttggcgctgt atttagataa cagatagagc
     4141 caacttttct cttgtgcgtc cgtggtaaac agcggcttat tttcattgag ataatgctgg
     4201 aggcgtgctt tttcgccacg ataagccagc gcggtcgcgt aagtaatata tgactgagga
     4261 tcggtgaaga tcccctgtga ttgcagtgct aggatccgat cgtccagctg cccggcaaga
     4321 agcacgtcaa accactgacg gcgttctgcc gcgcttaatg tgttctgctg gcgtgcttca
     4381 ttgtatagcg tatctgcctg ggaccattgt ttcaggtaga ttgcccgttg cagcagatcg
     4441 gttcgcagcg tttttccttc cggcgatgca gcaaacgtcg catcgttcag ttgcgctctg
     4501 gcgacaggta actgtgcaag ccgcagggca ttctgcccga cttcactgcg acaacgcagg
     4561 gtcggcgcag catcgcacgc tttttgctgg gcaagcagtt cttcaacagt tgtaacgctt
     4621 ttcacttcaa ccggaatagc cgccagactg cgctcaagtc gggcatctcc tgggtgacgt
     4681 ttcagttgat cctcaagcaa cagccgcgcc cggtcatcat gaccaaaatg gcgataggct
     4741 tccgcaaggt ataaagtcag cggaatatta tccggcacct gctggtgtat atattcaaat
     4801 tcgcggatgg cggttgcttc gtcgttattt ttctgtgcct tcagcgcctt atcgagacgg
     4861 ggataaataa caaaatggcg ataatcgctc agccccagct cttctgcgct ggtgccgata
     4921 ttgtctgcga gtgcgctggt actcaataaa gacgtcagca gtaaaccaga ccatccgatg
     4981 acgcgattaa ggttattctc cttcattttc ggactccagt tgcgcaacct gttctgtgtt
     5041 taaacctgct ttgagtaata gtgattgcat cgaaacttgt aattcgcgtt gaattgtcag
     5101 gacgcgatcc aacgtttcct ggctgataac gccttcggtg accaaaaact tgccgagcgg
     5161 cagagaactg cgttcatggc gcaataacaa cacgttaatt gctgaacgat taatatgacc
     5221 gagcgtggtc agtatttcgg cgaacaggaa ctgatgcggc acatattgcc gccagatttc
     5281 accggcctgc tgttccgtga gccactgatg ctgaaccgca ttgtacaaca ttgcccgcgg
     5341 atcgtgaccg cgtcggcgtg cataccagtg acgtaacccc gtgacaattt gtccccgcag
     5401 aacaatgaag taacgcactt tgcgtccgac tttacgcgtc agggccgcca gcgaaaccgg
     5461 gtcaatacca tcttcactgc cgacaattaa ctcatcattt tccagacgca gcggcagtac
     5521 cgcataatgc agcgccacgg aggccggcat ttcggcaatc agcgaggaag ggatctgcca
     5581 ggcatcgatg gattcccacg ccacgccgtt ttgctctgcc agcgcctgtg ccagctgctc
     5641 ggcgctaatc agcccctgca tcagcattga accgcccaag cgtagacctt cgacgcgatt
     5701 acgcagtgct gtatcgagtt
//
LOCUS       DAJTXQ010000197         5633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000197 DAJTXQ010000000
VERSION     DAJTXQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.198"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO515_003907"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO515_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6577705.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO515_003908"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO515_003908"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6577706.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO515_003909"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO515_003909"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6577707.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO515_003910"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO515_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6577708.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1397 a         1350 c         1528 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atc
//
LOCUS       DAJTXQ010000198         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000198 DAJTXQ010000000
VERSION     DAJTXQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.200"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..388
                     /locus_tag="OO515_003911"
     CDS             <1..388
                     /locus_tag="OO515_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577709.1"
                     /translation="AYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREG
                     FDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQML
                     ISQGKTRLQIQEELGISRATYYRLAK"
     gene            757..1419
                     /locus_tag="OO515_003912"
     CDS             757..1419
                     /locus_tag="OO515_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6577710.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1783..2660
                     /locus_tag="OO515_003913"
                     /pseudo
     CDS             1783..2660
                     /locus_tag="OO515_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2660..2950
                     /locus_tag="OO515_003914"
     CDS             2660..2950
                     /locus_tag="OO515_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577711.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            3271..3561
                     /locus_tag="OO515_003915"
     CDS             3271..3561
                     /locus_tag="OO515_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577712.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            3561..3980
                     /locus_tag="OO515_003916"
     CDS             3561..3980
                     /locus_tag="OO515_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577713.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            4148..4471
                     /locus_tag="OO515_003917"
     CDS             4148..4471
                     /locus_tag="OO515_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577714.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4901..>5485
                     /locus_tag="OO515_003918"
     CDS             4901..>5485
                     /locus_tag="OO515_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577715.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRL"
BASE COUNT         1796 a          983 c         1151 g         1555 t
ORIGIN      
        1 agcttatgca cgagaaggtg acaccgtagt tgtggtagct gtagaccgtc ttggacgtga
       61 cacaattgat gtgttatcaa cggttaaagc gctacaggct aaaggtgtga cagttatcag
      121 ccttagagaa ggatttgact tatcaagcgc tatgggtgaa gcaatgttag gcattatgtc
      181 aacgttggca cagcttgaaa gaagcttgat agcagagcgt agaaaggctg gtattgaacg
      241 tgctaaagct gaaggggtac atatgggaag acctgtaaaa gcttcttcag aggctgtaca
      301 gatgcttatt tcacaaggta aaacgaggtt gcagattcag gaagagctag gaatctctag
      361 agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat gtagctgata
      421 tttggctttt ttgccaaggg ggtcacatag ctggtgtgac gggtactaaa cacgtataca
      481 tcatgaggta gattctaccg aatcaactag acagatattt tcgaagattc ggggaagatg
      541 agattcaaag agggctagtc attttttata aaaattttca gactctgaaa agttactgta
      601 cagaatatat acagatatct ttaaagtatc tgtaaaaact tttaagataa aagatagata
      661 tcccagaaag gatatccgtt tagaaaacct gttaagcatc tcttaaaaga gagtaaacct
      721 tgtcaagagt gattgcaata aaaattttta tttcttatgt caattgagca tattttaatc
      781 gggaagcatc atgggggttc tgattactgg agaactccat ttttgttatt taagaactta
      841 caccgtgaat ttattttcag tctagatggt gctgcaactg agcatgacac gctgttacca
      901 cgattcaccg atgatataag ccgtcaatca tgggttagtg aaaaggtctt ctgtaacccg
      961 ccgtacagcg atattccgtc gttcctctta aaagcgtctg aagcggattt agtagctttc
     1021 ctgataccgc atagagccaa caccagctac tggcttaggc acatttacag caataaccac
     1081 tgtcatgaaa tccgtattct tcatcgtgca gttaaatacc ttccaccagc agggcataac
     1141 aggcttacta ttaggtcacc gtttccaagt gcagtcgttg tttttaaaaa agaaccacgt
     1201 aagcatgaaa ttacacagat ggtttgctgt gctgatacat tgctaccgtt aaccatcatt
     1261 aaccgtggtg gactaagagg aagacccacc atttacccac ctgagacgct cgacagcttc
     1321 ataaaactat acaggcaagg aaaacccatt aagtacattg cagatgctct cagaatgcct
     1381 ctgagcacgt catacaggat tgctcaaagg ttgagctaaa attctcataa atatggtcat
     1441 ttacaagaca ggtgattcag atgttaagaa gtatccctgt gaacaataaa tgaccaatca
     1501 gtaatcattc tgtgaaataa ttgttgcaat ttataatcgc atctgttata gtttctatat
     1561 gggctgataa aaaagttcat gtcttgaagt ggtaacactt ggggaggtgt cagatggttg
     1621 gcgtatatta acctctcatt cagggtatac ctcagcgttt taatcatcgt actgtttcag
     1681 gcatggactt ctttatcagt ccatcaatcc cccagatatt ttaatcacac aggctaatcg
     1741 gttttatccg gttggccttt tcacgtatga ggtaatttat gaatgtctaa atataaccca
     1801 aatcagtttt ataaatttga tgtgcgaatc tctcaacttt cagaagaaat taacgcagca
     1861 gcaagattga tttatatggt catggtccat agctgtgact tctatgaaga gaaatacaaa
     1921 tcatattcac caagtattga ccatctcgct tatatggctg gtgtatcaaa gagaacagca
     1981 atcagagcca ttgagacact tgagaaacac ggtttaattc aggtgcaaaa gaatcagggg
     2041 cgtcaaagca cttatcgtgt attcacactg gatgataagc cggaattact tgtaagacct
     2101 acatcacagg aagttgcagc ctcagtaaga caaaatgaca ccactaccag tgacaaaatg
     2161 tcatgggggt agtgacaaaa tgtcacccgt gttagagttt agataagtta ttagataata
     2221 atattacaga taagaataaa gagactggac ttaatgatga tattaaagaa gatgactttc
     2281 tttctttgga tgaattaaat gattttctct ctatgaatga atctgacctt gaagtaagct
     2341 ctaaaaagac ttctaaacaa gacagagaaa tcactgatgt gagtgttgat agtgaccata
     2401 aaaacacaca gtgccccgcc actgatgatg agttggaccc cttcgatgat gaagaggatg
     2461 aacgtgaaaa agccattaag agggcagaga agaaaaagag attcgagctt gcaagacaaa
     2521 agaacaaagt atcgggttta gacccattcg atgacagtga agatgattat tatctttcac
     2581 cgaaaaagaa acaaactcag gatgacaaga agcaaaaaat taaaccgaag atgtattttg
     2641 ccggtggagg aattaagtaa tgaaagaata tcacggtgaa aagagataca aagattacct
     2701 gttaaagcgt tacagcattt ctcgtgaagg tcatcttatg aaggatactc acggggaagt
     2761 ttaccggatt agaccaaaga aagaagggag gggttatttc ttttacgatg gtgtgacaga
     2821 tttaaagatt gacgctttga ggtttgcagt gctgtatcat tttgatatct ggaattcaat
     2881 ccaccagcta cgattaaaag acggtgaccc cagcaactta aaggccacca atattatcac
     2941 aaaacgttag tttgttttac catcatcaat cgtaattgtg atttcatatt tgaaaagctt
     3001 aatgctgaaa ttgaatcgtg tacttctggc gataatcatt tttaaaagct gatacacaag
     3061 aagcaccaga aacacaaagc aacctaagct tgcgaatctg tgagattcag caattgtttc
     3121 aagaaagccc attaattccc cttatttatc aaagggaatg acctgtatca gccccctaca
     3181 accatattaa catgtaaatg agttgttgac aatatgttat aaatatgtta aagttagtta
     3241 taagtcaaag attttaagaa ggaaaattta atgagacaca cagttacacg ccttttgcca
     3301 gaggcatatt ccagactggc agcaatcaaa gacaaatacc cagccatgag ccacacagca
     3361 cttgttggtg agattttgaa ggaacacata gcccttacac cgtttgacgc tatcaagggc
     3421 gttgcagagg gttctaaggc catcagaatc acaacgggcc aactggaact actcaaagca
     3481 cagtccgcag tggcaggttg ctcaatgatt caggtgttga gtgccaaggt tcatcaactg
     3541 gcggataagc tgggggttta atgctatgca catttgatta tcatgcgttt tatcgccata
     3601 gaggtcacct tgtccgtgag aataacggaa catggaaaga tttaactcgt aatgctgaac
     3661 ccttcacaca ccagcaatta aaatcccgct tctccagatg gaagaatgca ccagcagata
     3721 ctggtgattt tgagattatc cataccttcg attatgcctc agaagatgac cttcacgatg
     3781 caattaagct gattgaagac ttcaatacct cagaagatgt ggatggtgac aaatataaat
     3841 cccgtggtaa ccgctataac cgtgatgata tcactcaggc cataaatgct ggtcaatctc
     3901 agcgatacat tacagaaaaa tatggtattt cacggagaca gtatttctac attaagccat
     3961 tgattaataa agacaattaa aaataaccat ctaagaaacg gtagaatgca ctttacaggc
     4021 agagttgcac cttaatttga taaattacct taccagccag tgcaactcgc tctgtaaccc
     4081 gcatggttac tggcctcaga atggctattt taagaggtct gaggtgtgtt tcataggaga
     4141 tttaaaaatg aataattacc caatgcaaat tatggttgat aacgacactg ctatgatggt
     4201 gcaaagcttc attgatgcag gtatcgcaat taattttgat aaattactaa agctgatggc
     4261 tgataatgta gaaaacatct ctgactttat tcagtcggtg gagtttaacg agcctcgaat
     4321 gatgctacca attacagaca gcaacatgaa aagattggtt atcgagcaaa cgaacatata
     4381 cagtatttcc ccagagcaat ttttgaaagg tgctgtgaca atcctgtaca gtgataatat
     4441 tctggtgacg gattctgtga gggttcacta atgggtttaa taattgcttg gacgttgctt
     4501 ggcttatttg ttggttgtat tattaacaat gcggtaaata agaattgatg ttatcaacct
     4561 gttaaaaagc tgttgacaaa taaccttaag catggcataa ttttaattat gggagagcaa
     4621 ttctctctta tttttctttc aataatccga tggatggata ttctgagttg gaaaggtgca
     4681 atgcttttcg tcatggcctt tcaggttagt ctgagaggct atctcaaaaa gtactcttgt
     4741 tcattcatta ttgaatcctt tgcccttcac agaaatgttt agggcttttt cagtttttaa
     4801 aagcacactt ggcaggttct tgttcagtca gactcttcac ctctgattgc tgagtgtgct
     4861 tctataaatt gaaataacaa ataaactaag gggtgaacga atgattagaa atgaacatga
     4921 aatgcttatc gaacacagac caagtgcact tgatattcac gaattaactt tgcgtgaatc
     4981 ttttgataga gcttggcggg aatcacacga agtaaataaa ggtccatata ttccaccaga
     5041 accactcgag attccaagag ttgaaattga tttttctatg aacgaacggt gtgaacttga
     5101 tttgaagttg aagctacaac gccgatattt ccaagctatt aaagactctc aagaagcaat
     5161 gagaagtgtt tacgagaaaa ttcagtccaa gcttgaagac gaaattgacg aaatggttat
     5221 ttcgatggag cttcaaacca attcttatgc ttatttctct cgactagacc gtgacggttg
     5281 ggggtatgac cctgtagaga ttggtaaagt gattgcagat ttaccagaag gtcaaagagt
     5341 tgttcgtaca gaaagagctg tcagagcacc acatcgactg tctctaattg ttgctgataa
     5401 atcacaagct gagcttgaag aaattgccaa agagaaactg atgaaagcaa gagaaaatga
     5461 gattaatttg ctgaaatcaa gactg
//
LOCUS       DAJTXQ010000199         5476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000199 DAJTXQ010000000
VERSION     DAJTXQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.201"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(277..648)
                     /locus_tag="OO515_003919"
     CDS             complement(277..648)
                     /locus_tag="OO515_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577716.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(709..1206)
                     /locus_tag="OO515_003920"
     CDS             complement(709..1206)
                     /locus_tag="OO515_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577717.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1282..2070)
                     /gene="aadA1"
                     /locus_tag="OO515_003921"
     CDS             complement(1282..2070)
                     /gene="aadA1"
                     /locus_tag="OO515_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6577718.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2128..2652)
                     /gene="sat2"
                     /locus_tag="OO515_003922"
     CDS             complement(2128..2652)
                     /gene="sat2"
                     /locus_tag="OO515_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6577719.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2747..3220)
                     /locus_tag="OO515_003923"
     CDS             complement(2747..3220)
                     /locus_tag="OO515_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6577720.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3552..4088
                     /locus_tag="OO515_003924"
     CDS             3552..4088
                     /locus_tag="OO515_003924"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6577721.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4203..4529
                     /locus_tag="OO515_003925"
     CDS             4203..4529
                     /locus_tag="OO515_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6577722.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4717..4956)
                     /locus_tag="OO515_003926"
     CDS             complement(4717..4956)
                     /locus_tag="OO515_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6577723.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1426 a         1305 c         1219 g         1526 t
ORIGIN      
        1 tctctagata gcagggattt cttggccttg cctcatgctg atagcttgta aatatcacac
       61 gatccttagc cccacaaagt gagcatactc cgtagctttc atttatagaa atcgtcgtta
      121 gattgtcgca gcatgtgcac gttccgacta tttgctcagg aagcatcgcc ataccttggc
      181 ctaggtaaag aagatcagac tcgtagccac atcggtggca actgagcttg tacgcaatgc
      241 ccatctggtt gtctcacctg aatcgtaacg tcagagctat tcatccatcc attcggcatc
      301 aatgtatgcc actaagccga ccagctgacc gtttggactg aagtatgttg cctgcacccc
      361 acttgacatc tcatcaatac caccgcactg cgctaggcgc cggtcccact tgcccacaga
      421 agcgtcagga tcggctgagt tccccaagac ccttgtgagc catgcctcct ttaactcaac
      481 ctctcgaatg attgcgtcgg ccagctcctc cgcgttatct agttcgacat agtctggaat
      541 tcggacgtac gttctgaaca cagctggata cttacttggg cgattgtcat acatgacatc
      601 aacaatgtac ccgtttgtgt aaccgtctct tggaggttcg tatgacacta gtggttcccc
      661 tcagcttgcg actagatgtt gaggcctaac attttattag agagcaggct agttgcttag
      721 atacatgatc ttcaggccgt tatctgtcag ggcaagcgaa aattggccat ttatgacgac
      781 caatgccccg cagaagctcc catctttgcc gccatagacg ccgcgccccc cttttggggt
      841 gtagaacatc cttttgccag atgtggaaaa gaagttcgtt gtcccattgt tggcaatgac
      901 gtagtagccg gcgaaagtgc gagacccatt tgcgctatat ataagcctac gatttccgtt
      961 gcgactattg tcgtaattgg atgaactatt atcgtagttg ctctcagagt tgtcgtaatt
     1021 tgatggacta ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag aaatgtcgta
     1081 gttggatggg gagtagtcat agggaagacg agcttcatcc actaaaacaa ttggcaggtc
     1141 agcaagtgcc tgccccgatg ccatcgcaag tacgaggctt agaaccacct tcaacagatc
     1201 gcgcatagtc ttccccagct ctctaacgct tgagttaagc cgcgccgcga agcggcgtcg
     1261 gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta
     1321 gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata
     1381 agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     1441 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     1501 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     1561 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     1621 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     1681 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     1741 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     1801 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     1861 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     1921 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     1981 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     2041 cgatacttcg gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg
     2101 cgaagcggtg tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta
     2161 ccagtacatc gctgtttcgt tcgagacttg aggtctagtt ttatacgtga acaggtcaat
     2221 gccgccgaga gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg
     2281 tgtctctaat cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat
     2341 gagactgtgc gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga
     2401 ggctagatcg ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa
     2461 tgcgccatag caagcagagt cttcatcaga gtcatcatcc gagatgtaat ccttccggta
     2521 ggggctcaca cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac
     2581 ttcacgaaca atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac
     2641 cgaaatcttc atatgacgcc taacgcctgg cacagcggat cgcaaacctg gcgcggcttt
     2701 tggtacaaaa ggcgtgacag gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc
     2761 agatttggta actataattt atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat
     2821 tgctggggat ttcaggaaag taaacatcac cttccggctc gatgtctatt gtagatatat
     2881 gtagtgtatc tacttgatcg atcaggcttt tgtatatctc cccaccacct gaaacaatga
     2941 catgatccgt tattttcttt aggttggtta aagcatcttt aattgatgga aagatcaata
     3001 cgttctcatt gtcagatgta aaacttgaac gtgttacgac cgcatacttt cggttgggta
     3061 atgctcccat tgattcaaaa gtcttgcgtc caaccaacag ccattggtta taggtaatag
     3121 ctttaaacag gagctgttca cctttggcac tccatggaat atcagggcca ttcccgataa
     3181 ctccattctt cgatatagct accattagtg atagtttcac aattcttcct cagaggttaa
     3241 cattttatta gtaagcatgc tcgttttgtc acccgctgat gcttaccgtt aattaatata
     3301 atcacttgta ttaaatagac tttctttaaa aatacaagac actctgttat tacaaatcgt
     3361 gcatgccgtc tatcctgctt gcacgatgca cattatctca cccaaagaac tcataacatt
     3421 ttaatttatt tagcattaaa ttaaaactct accggtaaac aagcatctct aggcgtaaaa
     3481 aacaggcact tttttaaatc ccagctagaa taggctgtat aggcagacag ttgcaagaca
     3541 aggagcgttt tatgtctaac agtccatttt taaattctat acgcacggat atgcgacaaa
     3601 aaggttatgc gctgaaaact gaaaaaactt acctgcactg gattaagcgt tttattctgt
     3661 ttcacaaaaa acgtcatcct cagaccatgg gcagtgaaga ggtcaggctg tttttatcca
     3721 gcttagcaaa cagcagacat gtagccataa acacgcagaa aatcgcttta aatgccctag
     3781 cttttttgta caacaggttt ttacaacagc cgttgggcga tattgattat atccctgcaa
     3841 gcaagcctag acggctaccc tctgttatct ctgcaaatga agtgcaacgc attttgcagg
     3901 ttatggatac tcgcaaccaa gttattttta cgctgctgta tggtgcaggt ttgcgcatta
     3961 atgaatgctt gcgtttgcgg gttaaagatt ttgattttga taatggctgc atcactgtgc
     4021 atgacggtaa gggtgggaaa agcagaaaca gcctactgcc cacgcgccta atcccagcaa
     4081 taaaataact cattgagcaa gcgcggctta ttcagcaaga cgacaactta caaggcgtag
     4141 ggccatcgct gccttttgct ttagatcaca aatacccttc tgcttatcga caagcggcgt
     4201 ggatgtttgt ctttccctcc agcacgctct gcaaccaccc gtataacggc aaattatgcc
     4261 gccatcatct gcatgactcc gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag
     4321 gcatcgttag caagcgtgtc acttgtcata catttcgtca ctcgtttgct acgcatctat
     4381 tacaagcggg gcgtgatatt cgcactgtgc aagaactctt agggcataac gatgttaaga
     4441 ccacgcaaat ctatacgcat gtgttgggtc agcattttgc cggcaccacc agtcctgcgg
     4501 atggactgat gctacttatc aatcagtaaa agtagataaa tatctttatg tagccctgag
     4561 tagcccctga tactttttat ggtgaaaaag ataaacaatc atagagggga taaatatgag
     4621 ccagttctac cattgttcag ataagtttaa gcaagatact gtcaatcaca gtgaccattc
     4681 atggtcatta cacttcctcc agttgccaga caatgcctaa cataaaaacg caaaagccca
     4741 aaagtcagag cttaggtgtt ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt
     4801 agattgcgtg cgtttctgaa gcacattttc cttatacatg gctttatccc aggcagcttc
     4861 atcaagctct tggagtaaca agccagtaat aatgcgacac tctcgggttt gtagcgagcc
     4921 gctggtgaga tcggagttat agcggaaagg ggtcatttgt aattatttgt gggtggagga
     4981 tagttaactt attattgtag ttggaagagg ctgctttcca aacattagca cctcgtaatg
     5041 cttgttactg tatattaatt tatgctctta gcgcatgtta atgggctatt acaatgcata
     5101 atgtccacat caagataact gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct
     5161 gtccggaaat acgggatcac tgtaaagtgt tggtgaggca gtctaggagg gggatttaat
     5221 ttgggatact gatgaaagga ttatgatcag ttggatatag gatcgtacta agctgagcgg
     5281 caggttagct agattagtta accagataag tgaaatctag ttccaaacta ttttgtcatt
     5341 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc
     5401 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc
     5461 ccacaagggc tgccga
//
LOCUS       DAJTXQ010000200         5463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000200 DAJTXQ010000000
VERSION     DAJTXQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.202"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..116)
                     /locus_tag="OO515_003927"
     CDS             complement(<1..116)
                     /locus_tag="OO515_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6577724.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="OO515_003928"
     CDS             complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="OO515_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6577725.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2334..2969)
                     /gene="nth"
                     /locus_tag="OO515_003929"
     CDS             complement(2334..2969)
                     /gene="nth"
                     /locus_tag="OO515_003929"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6577726.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="OO515_003930"
     CDS             complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="OO515_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6577727.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="OO515_003931"
     CDS             complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="OO515_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6577728.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="OO515_003932"
     CDS             complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="OO515_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6577729.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5351..>5463)
                     /locus_tag="OO515_003933"
     CDS             complement(5351..>5463)
                     /locus_tag="OO515_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HCR6577730.1"
                     /translation="NAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1445 a         1384 c         1340 g         1294 t
ORIGIN      
        1 ttttcgagac gttttttcat taaatccaca ctgacgaggg taaaatcctt tccgctctca
       61 cgcagggtga tatgggaagc gagagagcag gcacccggtt tgtagaacaa tttcatcggt
      121 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaagccgct
      181 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
      241 ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca
      301 tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga ccataaactt
      361 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
      421 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
      481 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
      541 cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata ccagcgtcag
      601 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
      661 ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata gcggccagaa
      721 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
      781 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
      841 tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag gcaacgatca
      901 ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata accacgatac
      961 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc agccaggtgg
     1021 cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg tagttgatag
     1081 gctcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
     1141 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
     1201 cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg gacatgtagt
     1261 acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta gaaagcagag
     1321 aagatgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
     1381 cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
     1441 caccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
     1501 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
     1561 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
     1621 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
     1681 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
     1741 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
     1801 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
     1861 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
     1921 cagcgttaaa tttttcaaaa aacgtaatgg cagatttttt cactgcactg agaggggggt
     1981 gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga tagtccagtt
     2041 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
     2101 attattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
     2161 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
     2221 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
     2281 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
     2341 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
     2401 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
     2461 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
     2521 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
     2581 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
     2641 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
     2701 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttaacccct
     2761 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtaaag tttcgccgtc
     2821 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
     2881 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
     2941 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
     3001 cacgttcggc agctgcttca gtacggcgct ttttcattct ttcatcaatc aggtattttc
     3061 ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc agcaggaaag
     3121 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
     3181 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
     3241 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
     3301 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
     3361 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
     3421 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
     3481 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
     3541 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
     3601 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
     3661 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
     3721 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
     3781 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
     3841 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
     3901 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
     3961 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
     4021 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
     4081 ctggcgcagt taccagatag caactctgtg ccagcgtatt gttatagcgt tcggctggca
     4141 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
     4201 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
     4261 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
     4321 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
     4381 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
     4441 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
     4501 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
     4561 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
     4621 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
     4681 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
     4741 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
     4801 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
     4861 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
     4921 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
     4981 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
     5041 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
     5101 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
     5161 gtttgagtac gagagcttcg gctaacagag tactaaccga tgccaacagg atctgaacga
     5221 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
     5281 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
     5341 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
     5401 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
     5461 tag
//
LOCUS       DAJTXQ010000201         5306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000201 DAJTXQ010000000
VERSION     DAJTXQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.203"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2927
                     /locus_tag="OO515_003934"
                     /pseudo
     CDS             1..2927
                     /locus_tag="OO515_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3908..4876
                     /locus_tag="OO515_003935"
                     /pseudo
     CDS             3908..4876
                     /locus_tag="OO515_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            5134..>5306
                     /locus_tag="OO515_003936"
     CDS             5134..>5306
                     /locus_tag="OO515_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6577731.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVL"
BASE COUNT         1407 a         1210 c         1594 g         1095 t
ORIGIN      
        1 ccgggtgctt accgggatgg cggacgctga cgtaccggcg tgaggccgcc ggtgacctgg
       61 ccggggaaat caccgacgtg acggacggtg ccgggcggga gttccgtctg gtgctgacca
      121 cgcaggcgca gcgtgcggaa gaggcccgca cctcttcgct atcttcttct gacagttccc
      181 gccctctctc agcctcagcg ttccccgaca cactgcccgg taccgaatac ggccccgaca
      241 ggggtatccg cctttcggcg gtgtggttga tgcacgaccc ggcatacccg gagagcctgc
      301 ccgctgcgcc actggtgcgg tacacgtata cggaagccgg tgaactgctg gcggtatatg
      361 accgcagcaa tacgcaggtg cgcgctttca cgtatgacgc gcagcacccg ggccggatgg
      421 tggcgcaccg ttacgcggga aggccggaga tgcgctaccg ctacgacgat acggggcggg
      481 tggtggagca actgaacccg gcagggttaa gctaccgcta tctttatgag caggaccgca
      541 tcaccgtcac cgacagcctg aaccggcgtg aggtgctgca tacagaaggc ggggccgggc
      601 tgaaacgggt ggtgaaaaaa gaactggcgg acggcagcgt cacgcgcagc gggtatgacg
      661 cggcaggaag gctcacggcg cagacggacg cggcgggacg gcggacagag tacggtctga
      721 atgtggtgtc cggcgatatc acggacatca ccacaccgga cgggcgggag acgaaatttt
      781 actataacga cgggaaccag ctgacggcgg tggtgtaccc ggacgggctg gagagccgcc
      841 gggaatatga tgaaccgggc aggctggtat cggagacatc gcgcagcggg gagacagtac
      901 gctaccgcta cgatgacgcg cacagtgagt taccggcgac gacaacggat gcgacgggca
      961 gcacccggca gatgacgtgg agccgctacg ggcagttgct ggcgttcact gactgctcgg
     1021 gctaccagac ccgctatgaa tacgaccgct tcggccagat gacggcggtt caccgcgagg
     1081 aaggtatcag cctttaccgc cactatgaca accgtggccg gttaacctcg gtgaaagacg
     1141 cacagggccg tgaaacgcag tatgagtaca acgccgcagg cgacctgact gccgttatca
     1201 ccccggacgg caaccggagc gagacacagt acgatgcgtg gggaaaggcg gtcagcacca
     1261 cgcagggcgg gctgacgcgc agtatggagt acgatgctgc cggacgtgtc atcagtctga
     1321 ctaacgagaa cggcagtcac agcgacttca gctacgatgc gctggaccgg ctggtacagc
     1381 agggcggctt tgacgggcgg acgcaacgtt atcattatga cctgaccgga aaactcacgc
     1441 agagtgagga tgaggggctt gtcaccctct ggcactacga tgcgtcggac cgcatcacgc
     1501 accggacggt gaacggcgac ccggcagagc agtggcagta tgacggccac ggctggctga
     1561 gagaaatcag ccacctgagc gaaggccacc gtgtcgctgt ccactacggc tacgacgata
     1621 agggccgcct gacgggcgaa cggcagacgg tggagaaccc ggagacgggg gaactgctgt
     1681 ggcagcatta gacgaaacac gcctacaacg aacaggggct ggcaaaccgc gtcacgccgg
     1741 acagcctgcc gccggtggag tggctgacct acggcagcgg ttacctggcg ggcatgaagc
     1801 tgggcgggac gccgctggtc gagtatacgc gggacaggct gcaccgtgag acggtgcgca
     1861 ccctcgcacc ggacctggac atccgcatcc cgtatgccac cgacccggcg ggaaaccgcc
     1921 tgccggaccc ggagctacac ccggacagca cgctcacggc gtggccggat aaccgcatcg
     1981 cagaggatgc gcactatgtt taccactatg atgaatacgg cagactgacg gagaagacgg
     2041 accgcatccc gacgggtgtg atacggacgg acgacgagcg gacccaccac tacgacagcc
     2101 agcaccgcct ggtgttccac acgcggatac agcatggcga gccactggtc gagagccgct
     2161 acctctacga cccgctggga cggcgaatgg cgaaacgggt atggcggcgg gagcgtgacc
     2221 tgacggggtg gatgtcgctg tcgcgtaaac cggaggagac atggtacggc tgggacggcg
     2281 acaggctgac gacggtacag accgacacca cacgtatcca gacggtatac gagccgggaa
     2341 gcttcacgcc gctcatccgg gtcgagacag agaacggcga gcgggagaaa gcgcagcgcc
     2401 gcagcctggc ggagaagctg cagcaggaag ggagcgagga cgggcacggc gtggtatttc
     2461 cggctgaact ggtgcggctg ctggacagac tggaggaaga aatccgggca gaccgcgtga
     2521 gcagtgaaag ccgggtgtgg cttgcgcagt gcgggctgac ggtggagcaa ctggccagac
     2581 aggtggagcc ggaatacaca ccagtgcgaa acgttcatct ttaccactgc gaccatcggg
     2641 gccagccgct ggcgctcatc agcgaagacg gcgatacggt gtggagcggg gcgtatgatg
     2701 agtggggcaa ccagctaaat gaggagaacc cgcatcatgt gtatcagcca taccgtctgc
     2761 cggggcagca gtatgatgag gagtcggggc tgtactacaa tcgtcaccgg tactatgatc
     2821 cgttgcagag acggtacatc acccaggacc ccattgggct gaaaggtggg tggaatctgt
     2881 atcagtatcc gctgaatccg gtgagcagga tagatccgtt ggggttattc tcatgtgaga
     2941 gttgtagtca ttttatagat gatataaata ataaactgga taaccttccg tttaacccga
     3001 caaaaatgtt tacagctagt ttaaatactg ctaattcagc aaggctgtat gcaagtggca
     3061 tgacaaaatt cggtataagt gttgctgccg atggaacgat cattgctgcc ccagtcggtg
     3121 cagcttctat ggctttaggg gcgtggaata taaaatctgc gacagctgca tataacagag
     3181 caaatttaca attatcagaa tcacttcagg aaaatggtag agataggtgt tggagaaatt
     3241 tattgggtat tttaccttat ggtgagcagt ttgatgaccc tggtgaacca tcaatattag
     3301 acgtttatgg tgaaaagatt gagaatatca aagattctcc aatgaagttt tttgaagagc
     3361 ttgggacgct ggggttgtaa tgtgtatttt taatgatatt tttatgtttg tgtttattgt
     3421 tagtagtata cttgtaaccg tgcattttgt gtattcttta ttttttaaaa gtggaaaggt
     3481 aaaagataag gtaaaaaaat ggattaaagt aatgtgtgat ttattttggg ggataggata
     3541 gtggtgtgat atggataaat ataaattaca gtggctggca tatttcgacc tgacgccgga
     3601 gtagataaaa aaacagacag gattggagta tagcaagcgt ctgacaatct ctcgggacag
     3661 tagtatgatg aagaggcggg gttgtactat aaccgtaacc ggtactacga tccgctaaag
     3721 ggcaagtata tgactcaggg aaagatcatc agaagttcac tttttgtact aaataattcg
     3781 cattttatgt ttaaaaattg agatattcct cattacctga agctgttttt ttattgctta
     3841 tacataatca aatactcctt acataattaa ggagaacaaa atggaaatta aaaaattgat
     3901 ggaacatttt ttgaagcaat atggtgattt tgaaaatggc attcctgttc acgataccat
     3961 tgccagagtt gtatcctgta tcagtcctgc aaaatttcat gagtgcttta ttaactggat
     4021 gcgtgactgc cattcttcag atgataaaga cgtcattgca attgatggaa aaacgctccg
     4081 gcactcttat gataagagtc gtcgcagggg agcgattcat gtcattagtg cgttctcaac
     4141 aatgtacagt ctggtcctcg ggcagatcaa gacggatgag aaatccaatg agattacagc
     4201 cattcctgaa cttcttaaca tgctggatat taaaggaaaa atcatcacaa ctgatgcgat
     4261 gggttgccag aaagatattg cagagaagat acaaaaacag ggaggtgatt atttattcgc
     4321 tgtaaaagga aaccaggggc ggctaaataa agcctttgag gaaaaatttc cgctgaaaga
     4381 attaaataat ccagagcatg acagttacgc aatcagtgaa aagagtcacg gcagagaaga
     4441 aatccgtctt catattgttt gcgatgtccc tgatgaactt attgatttca cgtttgaatg
     4501 gaaaggactg aagaaattat gcgtggcagt ctcctttcgg tcaataatag cagaacaaaa
     4561 gaaagagcca gaaatgacgg tcagatatta tatcagttct gctgatttaa ctgcagaaaa
     4621 gttcgccaca gcaatccgaa accactggca cgtggagaat aagctgcact ggcgtctgga
     4681 cgtggtaatg aatgaagacg actgcaaaat aagaagagga aatgcagcag aattattttc
     4741 agggatacgg cacattgcta ttaatatttt gacgaatgat aaggtattca aggcagggtt
     4801 aagacgtaag atgcgaaaag cagccatgga cagaaactat ctggcgtcag tccttgcggg
     4861 gagcgggctt tcgtaatctt gccctgctga agagtttgaa agggcacgga tcctttcaaa
     4921 agaatacatt gagaaatata taagctaaag atgattagct gtatttcatg ataataatct
     4981 aatgagggat ttttctgaac atcaaattgc atcgaagtta gttagagtgc tgcgattgtc
     5041 ttgcacgtaa taaattcaga gagaaaatat agttgtgggt atgagaaaat aaccaaccta
     5101 atgtaaatca attcagatga agaggaataa accatgagcg gaaaaccggc ggcgcggcag
     5161 ggcgacatga cccgcaaggg actggatatt gtgcagggtt cagcgggtgt gctgataggt
     5221 gcgccgacgg gagtggcctg ttcggtgtgt ccgggaggga ttacctatgc taacccggtg
     5281 aacccggtgc tgggtgcgaa ggtgct
//
LOCUS       DAJTXQ010000202         5271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000202 DAJTXQ010000000
VERSION     DAJTXQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.204"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..461)
                     /locus_tag="OO515_003937"
     CDS             complement(<1..461)
                     /locus_tag="OO515_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6577732.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNY"
     gene            complement(461..1381)
                     /locus_tag="OO515_003938"
     CDS             complement(461..1381)
                     /locus_tag="OO515_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6577733.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1519..2418
                     /gene="pgrR"
                     /locus_tag="OO515_003939"
     CDS             1519..2418
                     /gene="pgrR"
                     /locus_tag="OO515_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6577734.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2755..4368
                     /gene="mppA"
                     /locus_tag="OO515_003940"
     CDS             2755..4368
                     /gene="mppA"
                     /locus_tag="OO515_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6577735.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4419..>5271)
                     /gene="ynaI"
                     /locus_tag="OO515_003941"
     CDS             complement(4419..>5271)
                     /gene="ynaI"
                     /locus_tag="OO515_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6577736.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1426 a         1200 c         1223 g         1422 t
ORIGIN      
        1 tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt aatactcact
       61 actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc acccccacac
      121 actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc ataatcgata
      181 acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg ctccccacca
      241 gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc agccaatgcc
      301 ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg gacgctgata
      361 atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt ggcagcaata
      421 tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt tcctcaaaga
      481 acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa agatccatat
      541 ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga tcgtacaggt
      601 cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc ggctgagtca
      661 ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg ctgcgcaaag
      721 tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac tcccaggcct
      781 gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc ataacggcat
      841 attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg tatggcaggg
      901 catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca atattgaccg
      961 cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg gctgtatatc
     1021 ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg
     1081 tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt tctaactgac
     1141 gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg acaaatccct
     1201 tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc ccacctgggt
     1261 gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg aaatagatca
     1321 ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta ttattcatca
     1381 ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac tatagatatt
     1441 gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa tgattaatag
     1501 attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
     1561 gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc gcagtcagct
     1621 ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
     1681 acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct tggccctatg
     1741 ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg cccatccggg
     1801 acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc agcaatgcgc
     1861 acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta tggtttgacc
     1921 gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat ggataaagat
     1981 atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc accggattat
     2041 ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca ggcaattaat
     2101 ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg tggacgtgaa
     2161 gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat cattgatgct
     2221 gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg ggctattaaa
     2281 gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg ttatcacctg
     2341 tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga taggctgaag
     2401 tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc tcttaacgta
     2461 ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg cgacagatgc
     2521 tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg ttcggtgttc
     2581 tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt aatatattga
     2641 tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt tgcatttctt
     2701 taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa acttatgaag
     2761 cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct ttcgtatgct
     2821 gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg ccacattaaa
     2881 gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat tcaggtcatt
     2941 cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt ccccggcgtt
     3001 gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg cgataacgca
     3061 aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg gcagcgtctg
     3121 gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg aatcaacaac
     3181 gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac cgcagttgat
     3241 gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa tttaaccgct
     3301 aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga gtggacgaaa
     3361 cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt caatgaaaaa
     3421 ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact gcaaaaagtg
     3481 accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc gggggatatt
     3541 gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga tattccgggg
     3601 caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac gcaaaaaggg
     3661 ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg ccgcctgatg
     3721 accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc agatgttacc
     3781 gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga actgaatgcg
     3841 caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct gaagctgacg
     3901 cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc atcgatgtgg
     3961 aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac ctatatcgat
     4021 agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga ttataatgaa
     4081 ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc acgctttaac
     4141 aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt taaagcgcgt
     4201 aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat tgcaccaatt
     4261 tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc cattaataac
     4321 ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg tgatgggaac
     4381 tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg ttccggcggt
     4441 gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc gtgtgactgc
     4501 acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc agcccatacc
     4561 gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa ctggttgaaa
     4621 taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat ctcacgtacg
     4681 gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat ggtcgtggta
     4741 atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa cagcgagttc
     4801 ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca gccaatttcc
     4861 gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat actgaaagga
     4921 cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt accggccata
     4981 ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct catgccgaaa
     5041 tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat gcgcgacata
     5101 atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa ctgattaatc
     5161 agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat aaaatcgata
     5221 acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat a
//
LOCUS       DAJTXQ010000203         5219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000203 DAJTXQ010000000
VERSION     DAJTXQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.205"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(291..575)
                     /locus_tag="OO515_003942"
     CDS             complement(291..575)
                     /locus_tag="OO515_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577737.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="OO515_003943"
     CDS             complement(593..790)
                     /locus_tag="OO515_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577738.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="OO515_003944"
     CDS             1002..1298
                     /locus_tag="OO515_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577739.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="OO515_003945"
     CDS             complement(1843..2076)
                     /locus_tag="OO515_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6577740.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="OO515_003946"
     CDS             complement(2083..2613)
                     /locus_tag="OO515_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6577741.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2591..3349)
                     /locus_tag="OO515_003947"
                     /pseudo
     CDS             complement(2591..3349)
                     /locus_tag="OO515_003947"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="OO515_003948"
     CDS             complement(3291..3614)
                     /locus_tag="OO515_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6577742.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="OO515_003949"
     CDS             3679..3870
                     /locus_tag="OO515_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6577743.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1367 a         1434 c         1118 g         1300 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgcatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgttc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgcacccg gtaaatttta accacatgca tagctatgca gccatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagca ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct catagaatgc tcctttcagt ctgatattct
     2641 tcccgccgtt cgggtctgca atgctgagac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagggagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattcccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca agcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttcc agtaaccggg catgttcatc atcagtaacc
     3601 cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta cccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 atcagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcacgtcagc gggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcgcc atcagtgcgg atggtatgaa aagtgcgcca taccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca gccgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ttaccgttta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catggggata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAJTXQ010000204         5080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000204 DAJTXQ010000000
VERSION     DAJTXQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.206"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..960)
                     /locus_tag="OO515_003950"
     CDS             complement(25..960)
                     /locus_tag="OO515_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6577744.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            1472..2581
                     /locus_tag="OO515_003951"
     CDS             1472..2581
                     /locus_tag="OO515_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577745.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADECIKKTNIYFDDNFLNIYKRSQDRAPQIISDLKKH
                     NIEIGEKLNDCRKKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQL
                     LQYAYINYSKNQIPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(2777..4327)
                     /locus_tag="OO515_003952"
     CDS             complement(2777..4327)
                     /locus_tag="OO515_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6577746.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            4499..4945
                     /locus_tag="OO515_003953"
     CDS             4499..4945
                     /locus_tag="OO515_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6577747.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
BASE COUNT         1407 a         1231 c         1118 g         1324 t
ORIGIN      
        1 aaaggcgctg ttatctgata taggctattt gtgcattgcc caattacgga tcgtattacg
       61 atgtacattc agcatcgccg caatagcttt ctgagaatat ccattctcca gcatacatag
      121 cgccatgaaa cgcttgtcat cgtaggcttc gcctttagcg atgccacctt tccggcctct
      181 cttggcctgt atttctggtg tgtgcgtatc agccacatac tgtgcgaaac tttccggcgt
      241 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt caggcggaga
      301 aagcggaacg ggaagcgatg cgttgtacat ttcgacacgc tgaatcacag catcaagcca
      361 ctgtgagaat gctggccagc cctgccgaat cgccctgtaa gcccatttgc gcgtcatttc
      421 gaacagatgg cagtttcgcc ccagcccgta atgtttatcg acgctacgac gctctgaggc
      481 tctcaaatcg agataatcag ccagttcatc gagggcgtag gcttcctcgc gccactccat
      541 cacctgccat tccaggtgga acggattttt gcaaatcagt ccgctgtagt tcacatctgc
      601 gtcaagtttc tcacacagcg cacgttcaat tgcagcggcg tatttcagcg ccttaaccga
      661 cgcatcaggc gcggttctca cggcaagatt gagcgcgtag agcaggtgag cgtgcccgtt
      721 ctcgggattt tttacggtga tgttcggcgc gggggcgttc cggtcgctcc agtcaatcgc
      781 agctcctacg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct gattttgctg
      841 gatgtagcga gcaagcagcg cacgcccttt gccggagata cgaagaccga aagcgagctc
      901 atccgtgtga tagggacggt gaggtaaatt ttcttcgaag tattgaagcg cggcgctcat
      961 gtgttacgat tccttccagg ttatcgaccg acatcgcccc tgtatcttcg tgaaagtctt
     1021 gccggataga cacgaatttc aggggcgttt gtttttcagt cgcttacact acccccaacg
     1081 acaaaacctg tcgagtcttc ccgcaaaccc gatccattta ccataactcc cgttgtgcgc
     1141 cccccttaaa acgccctcag tcgctttctg tcgcaaaaaa cgcaaaaaga cagggaaaca
     1201 acaggccaga ccaaaaaaac gcgccagaat tgttttcagg gggctgcata actatgcacg
     1261 aaagcgattc atgtccggtt tcactcgttt tgaacagcga acaggtcaaa acagcaaact
     1321 ttctcatcca gaaaaaggtg aactggtgcg aaatcttttc gaactgcgta agacgttgag
     1381 cacaaacata gtaagtcggt ttatcggtat gtatggattc cattaacata tcgcctgtat
     1441 taatatttcc tcgttattgt tagaaataaa aatgggaaaa atgaatatta actttaaaca
     1501 tatccttcct ttattgtcgt cattttttat ttcaggatgc gactctgatg gaataagcaa
     1561 ggtaaagtct ttcgtatatt acgacattga cgactcaatg actgttggta aagccttcga
     1621 tacaaggagt gattgcatca atggcacatg gagtgaaaaa gaggatgatc gaggaagaac
     1681 catcgtcaca tatacctgtg atattagtga aagtggccta aaaataataa attcagccat
     1741 tatgcaagaa cctgaagata aggctgaata ttctatagca caaaataaca acagcataaa
     1801 atcaaccaat gaatcactag aggaatacaa acagaaaacg cctctggtgg aaaaaacaaa
     1861 agaggtcata atagatcata taagaaagct aaacagtgct tttaatgaag atccaatgat
     1921 atatagcaaa ctcacaatta gcccttattt agacagaatg ctatatctaa aggaccataa
     1981 tgacaatgcg ctaaatgaaa tttgtggtgg gtacgaatac attcaatctg ccgatagtta
     2041 cagttggcgt gatgtaagtg aagagtatgc aaaagaatca tgcgaaaagc atatatataa
     2101 agtatatcaa tcgtttaaga aaaatgcatc accattaata actaaaaact tccccggttt
     2161 ctacgaacgt gtccccccct gtgagaacgc agatgaatgc ataaaaaaaa caaatatata
     2221 ctttgatgat aactttctaa atatctacaa aagaagccaa gatagagcac ctcaaataat
     2281 ttctgattta aaaaaacaca atatagaaat aggtgaaaag ttaaatgatt gtaggaaaaa
     2341 actaaatatc tcagatgtca aattaaccta ttactggttt gtaacagata ccggaggagt
     2401 tgattattta gatggggtgt tgacatataa ttttcaggga aagaatagag ctgaaaactt
     2461 tcataaacaa ctattgcaat atgcatacat taactacagc aaaaatcaaa tacctcggga
     2521 ctttgtaaca tcaataaaaa atagtattta ttataaaata gaagattgca ctaagtttta
     2581 gtccttccct tacaggtggt tacacatata accacctgca aatatttaac acctgttaac
     2641 ttcattaatc tgaactgtga gtaaatcaaa acgacaattt ttctcattca gaaaacgttt
     2701 cacgggacgc ggaatttttt cgcgttccgt gaaacgttgg cggggttcgg ggcgcagtcc
     2761 cgatattgct acattttcaa cgctccatat cttgatcacg ttcgttacta cgtgataccc
     2821 ccctggccca ggatcgctgt atacacagtg ctttttcctc ctgttgctgc cgttcctgcg
     2881 ccatttctgc cagtaactca tgtcgcattt gagctttttc atctccagat atactgagtt
     2941 caggctccag agataacatc tggctacggg caacggatac ctcttcagca accttttgcc
     3001 tggtgttctc taaagatgcc gccgccagaa tctcgcgttc tctgtacctg tccagctccc
     3061 caacgtactt atatccctcg gtacgcttaa aagcagcgtt ttctttgtcc cactcggatt
     3121 cggcttgctg tagcttgttc ctgaattcat tcctttcatg ttcagcggtt tccagcacac
     3181 ttttttgctc cagaatacgc gcatcaatct cccttttgtc agccccccga aacaggccac
     3241 gaatcccatt caaagcattg cgttgttcct gtagctcccg cagctccctt tgtgcggaaa
     3301 caacattacg ccccagtttg tctaaatacc gtgccccttc atatattcgt ctactccatt
     3361 cggaaagaga gtctttttta gctttcgcat tctgaagcag cttatgttct ttgagcgcat
     3421 tgggattggc atcccctaaa cgagcaagcc ttgcctctcc ctgaatgacc gttttcataa
     3481 ccttgcggta ctgaagcaat aactccggtg aacgggctga acgaacaggc tcaactgcct
     3541 tgattttttg cttttgctcc tgttcctgct gaatggcatt tgcggcatct ctggcggtct
     3601 tgagttcccg ctcggcattc ctccgttcct ggattgcaac aatctgctcc ccattcagac
     3661 tggcggcctg aacggggcca agatgaactt ccggcgtgcg attgataccc tgttctttca
     3721 gagaacgatg atctacgcgc gactcgtgac cgtgtcgcgc aaggtgctca ttctgcacat
     3781 ctgcccagcg ggaacgcagc gcaacaaggg cggcttttcg ctctccggct gtctggggaa
     3841 tactcgcttt ttttgcgcct ccccgctctg gtgatttgct gttggcacgt ttaaaaaatt
     3901 gctgtggatc gcgttcaata ccatcattaa tccgctcgca aaacatgata tgcgcgtgtg
     3961 gctgttcctt tccgtcgatt gcacctggcg gattgtggat cgcgtactgg taggcatggc
     4021 gatcgccaag ttccttttgc acgaaatctt caacaagttc tatccgttgt tcgcgtgtca
     4081 gttcacgcgg taacgcgatc tcaatttcac ggtatgcagt cccgtttttt cgttcaaatt
     4141 catctgcggc tttccatagc tctgcgggat ttttcttcgc ccatgatggc atgttaccgg
     4201 atgacgaaaa ttccagatcc tcccgcgatt tgtaactctt atattcgccg gatctggaaa
     4261 tgtaatcagc atgttttaag gcactgcctt ttgctccagc tttgacagtg caatggtagc
     4321 ttgccatctg ccttttagcc ctccgcagga gcgcacagtt tctcgacgaa ggagagaaac
     4381 tactaagtgc gcatccctga attaatcatg gatgatttta cggtctcaca cgcaggcaaa
     4441 caaggtgata ttatgcataa taacaactca cattaacggc aaaagtgcag aggtaagaat
     4501 gacaaatcgt gaagagtggt taagtgcaaa aattgcctac atcaacggac tgaaatcacc
     4561 atcagagcag cagagattac ttgtgttact ggcagagaaa aaaaaccgaa caacgacgga
     4621 tgaaaaaacg ttgtccgcac tgatacgggc agaaaaaacc gcagagaaag cagccgcagc
     4681 aaaagccaga gtaacggcaa tcatagcggc agaacggaag gcagccgcaa gggcggaacg
     4741 aaaggcgcgg gatcatgaac tctacaaggc cgcaggcctc atgattgtgg caggactggt
     4801 ggacagcaaa accggaaagc caaagtttag tgctgccgaa ctggtaggcg cactcgcagg
     4861 aatcgcagag ctaccgcaca accatccaaa atggcaggaa tgggaaaaac gcgggaaaga
     4921 gctactcacc aaagacagtg cgtaagagcc tccgttcacg ttccagttcc ctggtgcttc
     4981 ctcgctcatt cgctcgctac gctcggtcat gagactgcgg cgagcacgac cacctcaatc
     5041 atacggtaag ccgcaccatt gctgggctga aaggagaaaa
//
LOCUS       DAJTXQ010000205         5044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000205 DAJTXQ010000000
VERSION     DAJTXQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.207"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="OO515_003954"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="OO515_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="OO515_003955"
     CDS             1595..1903
                     /locus_tag="OO515_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6577748.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OO515_003956"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OO515_003956"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6577749.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="OO515_003957"
     CDS             complement(3750..4700)
                     /locus_tag="OO515_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6577750.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="OO515_003958"
     CDS             complement(4839..>5044)
                     /locus_tag="OO515_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6577751.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1229 g         1211 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttt aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAJTXQ010000206         4872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000206 DAJTXQ010000000
VERSION     DAJTXQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.209"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(348..1391)
                     /locus_tag="OO515_003959"
     CDS             complement(348..1391)
                     /locus_tag="OO515_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6577752.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1803..2987)
                     /locus_tag="OO515_003960"
     CDS             complement(1803..2987)
                     /locus_tag="OO515_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6577753.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3287..3381)
                     /locus_tag="OO515_003961"
     tRNA            complement(3287..3381)
                     /locus_tag="OO515_003961"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Sec,seq:tca)
     gene            3674..>4872
                     /locus_tag="OO515_003962"
     CDS             3674..>4872
                     /locus_tag="OO515_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6577754.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1273 a         1066 c         1114 g         1419 t
ORIGIN      
        1 atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa atataggggc
       61 aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac ccgctggcag
      121 ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt attacgaaag
      181 aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat ccttttgcaa
      241 aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac agtgttatta
      301 ttggataata aattattact tgttttaaag ttacactaat gcaatatcta ctgatactgg
      361 atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca gatatattca
      421 ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc aattcttttc
      481 cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc ttcctgaatc
      541 agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga atggttatag
      601 agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga gtatgttgca
      661 tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc aagtggaaat
      721 ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa gccatggcga
      781 aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc gagttcatac
      841 cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg ctcatgatcg
      901 ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc ttcaggattg
      961 tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa gtgcacattt
     1021 gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg catccagatt
     1081 atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc tttaagagag
     1141 gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag atgagcgtgt
     1201 atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat gtaactcgtc
     1261 tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc cagcaatggg
     1321 ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc aaagcataat
     1381 ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag cataaaagtt
     1441 gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac gaagcccctt
     1501 catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt tataatgatg
     1561 tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta aaggtatcga
     1621 aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt gtttttaatt
     1681 atgtttttgt aataagtttg aagacgcaaa aaaaataata taataacaaa aatcctccag
     1741 ccttgtaggc cagaggataa aatacttaat tgaggaaaat atcagggaaa agtatttctg
     1801 acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg catcatttca
     1861 cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt tttatccaca
     1921 tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag aattgtactc
     1981 atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa ttcaattact
     2041 tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat aaagactagg
     2101 ggatgatgtt ttgagattgg ttcaagctgc ttaaatagca taatgacctg ctcagagagc
     2161 ggcacaatat gaggtcgctt catcttcatt acttctgccg ggatctccca caattttgtc
     2221 tcaaaatcga tatcttccca gcgtgcaaaa cgcagttctt gtgttcgtac gccggttaac
     2281 ataatgatct gagtagctgt cttagtgatt atacttccgg tataacccgc cagatcgttg
     2341 aggaagtggg gaagttcatt agcagtaaga aagggaaaat gtactttttt cggtgtagct
     2401 aaagcactgg caagatcggg ggctgggtta tagtctgccc ggccagtaac aattgcatag
     2461 cggaacacct caccacagcg ctggcggacc ttgcgcattt tctccagagc tccacgtttt
     2521 tccattttac gcagtgcttc cagtaactcc attggtttga tttcggcaat agggcgcttg
     2581 ccaatgtagg gaaaaatgtc tttttcaaag gtatcaatga tttcatcacg atagcgtaat
     2641 gaccaccgat cagcttttga ctggtgccat tcgcgagcaa tagcctcgaa cgtatttttc
     2701 agactcattt gctgcgcgat tttttcttcc ttttttgcct cccctggatc gcctccagca
     2761 gcgaggagtt tcctggcttc actgcgtttc tcgcgagcat cggctagcgt tacatcgggg
     2821 tatacgccaa tagaaagcat cttttcttta ccagcaaagc ggtacttcat gcgccagtat
     2881 ttagaaccat tagtgttaat cagtagatac ataccgccgc catcagcaag cttatagggt
     2941 ttgtctttgg gttttgcggt tttcactttg atatcggtta atgccatgac atcctccggc
     3001 ttttgatgaa ttcatgttcc tgagggggat ttctgccagt aactaacctg atacccccaa
     3061 tacacccctt gtataacaaa gatattgtta aaagccgttg ttatgtatca catttttcct
     3121 attatggggg tgcttgtatt tcaataggaa ataacacccc ctaaagtacc cccaaaagaa
     3181 acttgatatg gggtgaagta ggttgacctc aggatacaaa taaatactga tatacaaggg
     3241 attagaagag atttattgac attgggagag ttcagaagag gtaaactggc ggaagatcac
     3301 aggagtcgaa cctgcccggg accgctggcg gccccaactg gatttgaagt ccagccgcct
     3361 caccggagac gacgatcttc cgcgcctcga ttgctacatg gaggcggggc gcattatagc
     3421 tacttccttg agtttctaca tcccccagat cagttttttc cgcctaatac acgcagcgta
     3481 ttatcacgaa agcattataa ctcatataaa acccatattt tacaatgata tatgagcgga
     3541 attaacgcta ccacgatcac ataaaacaag aatcataaat taataaccag atatcggaat
     3601 attcgctctc acagggatgg tttacaaaat gcgttatcgg tctcagcacc cctatagcat
     3661 caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg gttatggcat
     3721 gggagacgcc gccagccaca ttattttcga taacgtaatg ttatatatga tgttctttta
     3781 taccgatatt tttggcattc ctgccggatt tgtcggaacc atgtttttgg tcgctcgtgc
     3841 actggatgcg atttccgatc cttgcatggg gttgttggcc gatcgaacgc gctctcgctg
     3901 gggtaaattt cgtccgtggg tactgtttgg cgcactgcca ttcgggatcg tctgtgtact
     3961 ggcctatagc acgccagatc tcagtatgaa cggcaaaatg atctatgcag caattactta
     4021 caccctactt accttacttt ataccgtcgt caatatccct tactgcgcat tgggtggtgt
     4081 aatcaccaat gacccgactc agcgtatctc gctgcaatcc tggcgttttg tgctggcgac
     4141 cgcgggaggc atgctttcta ctgttctgat gatgccactg gttaatttaa ttggcggtga
     4201 taataaacca ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg cattcatgat
     4261 gctggcattt tgtttcttca ccactaaaga acgcgttgaa gcaccaccta caacaacgtc
     4321 tatgcgggaa gatttacgtg atatctggca aaacgaccag tggcggattg tcggtttact
     4381 aaccattttc aatatcctgg cggtgtgcgt acgcggtggg gcgatgatgt attacgtcac
     4441 atggattttg ggcacgccgg aagtgtttgt cgcttttctc accacttatt gcgtgggtaa
     4501 cctgattggt tccgcactgg caaaacctct gaccgactgg aaatgtaaag tcactatctt
     4561 ctggtggacg aacgccctgc tggcagtgat tagcctcgcg atgttctttg ttcccatgca
     4621 ggccagcatc actatgtttg tcttcatctt cgtgattggt gtgttgcatc aactggtgac
     4681 acctatccag tgggtaatga tgtccgatac cgtcgactac ggcgagtggt gcaatggtaa
     4741 acgcctgacc gggatcagtt ttgctggcac gctgtttgtg ctcaaactgg ggttggcctt
     4801 cggcggcgct cttatcggct ggatgctggc ttatggcgga tatgatgcgg cagaaaaagc
     4861 gcagaacagc gc
//
LOCUS       DAJTXQ010000207         4798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000207 DAJTXQ010000000
VERSION     DAJTXQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.210"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="OO515_003963"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="OO515_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6577755.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="OO515_003964"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="OO515_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6577756.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="OO515_003965"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="OO515_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6577757.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO515_003966"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO515_003966"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6577758.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO515_003967"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO515_003967"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6577759.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4798
                     /locus_tag="OO515_003968"
     CDS             4447..>4798
                     /locus_tag="OO515_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6577760.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1162 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaa
//
LOCUS       DAJTXQ010000208         4796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000208 DAJTXQ010000000
VERSION     DAJTXQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.211"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..81
                     /locus_tag="OO515_003969"
     tRNA            6..81
                     /locus_tag="OO515_003969"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO515_003970"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO515_003970"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO515_003971"
     CDS             complement(299..1057)
                     /locus_tag="OO515_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6577761.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO515_003972"
     CDS             complement(1065..2168)
                     /locus_tag="OO515_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6577762.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="OO515_003973"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="OO515_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="OO515_003974"
     CDS             complement(3427..4452)
                     /locus_tag="OO515_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6577763.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAJTXQ010000209         4775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000209 DAJTXQ010000000
VERSION     DAJTXQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.212"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(51..494)
                     /locus_tag="OO515_003975"
     CDS             complement(51..494)
                     /locus_tag="OO515_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6577764.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="OO515_003976"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="OO515_003976"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6577765.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="OO515_003977"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="OO515_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="OO515_003978"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="OO515_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1257 c         1208 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaa
//
LOCUS       DAJTXQ010000210         4701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000210 DAJTXQ010000000
VERSION     DAJTXQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.213"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(528..905)
                     /locus_tag="OO515_003979"
     CDS             complement(528..905)
                     /locus_tag="OO515_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577766.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            1170..1625
                     /locus_tag="OO515_003980"
     CDS             1170..1625
                     /locus_tag="OO515_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCR6577767.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            1625..1795
                     /locus_tag="OO515_003981"
     CDS             1625..1795
                     /locus_tag="OO515_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577768.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1788..2078
                     /locus_tag="OO515_003982"
     CDS             1788..2078
                     /locus_tag="OO515_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6577769.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            2075..2437
                     /gene="rusA"
                     /locus_tag="OO515_003983"
     CDS             2075..2437
                     /gene="rusA"
                     /locus_tag="OO515_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCR6577770.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            2434..2574
                     /locus_tag="OO515_003984"
     CDS             2434..2574
                     /locus_tag="OO515_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR6577771.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            2660..3043
                     /gene="quuD"
                     /locus_tag="OO515_003985"
     CDS             2660..3043
                     /gene="quuD"
                     /locus_tag="OO515_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR6577772.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(3232..4314)
                     /locus_tag="OO515_003986"
     CDS             complement(3232..4314)
                     /locus_tag="OO515_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6577773.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT         1324 a          978 c         1048 g         1351 t
ORIGIN      
        1 tggtaaagaa gataataaag aataatattt agggttatag gtcttagccc attttaaatg
       61 gaaggcagac atgcagccat gcatacttcg catggcaaca taattattta gacaaactat
      121 ctaatttttg cgtcgctttt tgtgttaggt cgctgtaact gataaaccct acgttagcac
      181 ctacaattaa cactaagaac actaagctgc atatggatag agtggcagat ttatgcttat
      241 ggaaaacaaa atatgctact attgcactta atatgaagga aatgaataga aatatagatg
      301 ttgatgtttt tagatatttt tcaggaaata atgggtgagc aataatgtat acaagattaa
      361 gtagtatata aattgctacc ggaatgaaaa tcatgctcca gtgtgtatct tttttgggag
      421 ggattggctg caaataatca gacaccgttt ttccatcagt ttcaagaaga attactagat
      481 ctcttacttc accactgact tgagtgcatc cttagtagta atgttaatca ttttttccct
      541 ccaaatacga tgccctctac gtctttggaa gctttttgtg atgaccactc ctctgaatca
      601 ggtgatttaa ttaaaaatat tgtatctcca tcaaaaatcg tatttgttcc gtaaaaattc
      661 acatttgata gatcaccacc aaaaaccctc tgcccactcg aggtaattgc ctcacctaat
      721 ttagtaaaat taccagcaat agatgatttt ctttttccac ttctattcaa agtggcttta
      781 attacaccac caccatcctt agagttaaat aattgaatgt ccactccagc agttggttgt
      841 atatattttg aaagctcttt cttgaacttg ttaatatatt tatctttgtt ttctttgttg
      901 gtcattggtt agatccatag ttctggctgt gattaggtga aagtatacaa ggtttttaaa
      961 gcgaagatca ctgttttggc ttcaagatag ttttgattta tcgtaatcaa tccgccataa
     1021 tcctctcatc ggagcctgaa caactccggt gacttctgcg catttaaggg gacttaaatg
     1081 cgaccacaat ctgaactcat caccttgtca cagatgcaga aatgcacctg cgattttctg
     1141 cattctgcgg tttccgttag ggaggccgta tgactctgcc agtagacggc atcaaactcc
     1201 atcgcggtaa cttcgcggcc atcggccagc agattcagcc attgctggat gccgggcaat
     1261 gcttacgcct gcaggttaag ccgtggcgcg agaagcgcag cctgtcgcag aacgcgctca
     1321 gccacatgtg gtacacggag atcagcgaat atctcatcgc ccgcggcaag accttcgcta
     1381 cgcctgagtg ggtcaaagac gcgatgaagc acacctatct cggctacgaa agcaaagacc
     1441 gtgtagatgt cgtgtccggc gaggtcacca ccgtccaatc cctccgccat acgtcagagc
     1501 tggaaaccgg cgagatgtac atcttcctgt gcaaagtcga agcctgggcg atgaatatcg
     1561 gctgccacct gacaattccg cagagctgtg aataccagca gctgcgcgat aagcaggagg
     1621 cgtaatggct acaccgctta ttcgtgtcat gaacgggcac atctacagag tatcaaatcg
     1681 tcgtaagcgt aagcctgagc tgaagccatc cgaaatacca acactgctcg gatataccgc
     1741 tagcctggtt gataaaaaat ggttgcgact ggcagcaagg aggaatcatg gctgatttga
     1801 gaaaagcagc gcgtggtcgg gaatgccagg taagaatccc tggcgtatgt aatggcaatc
     1861 ctgaaacgtc agtactggca catatccggc tggctggatt gtgcggtacc ggtatcaaac
     1921 cgccagacct gattgccacc attgcatgtt ctgcctgtca cgacgaaatc gaccgccgca
     1981 catattttgt cgatgctgag tatgcaaaag aatgcgcgct ggaaggtatg gcgagaacgc
     2041 aggttatctg gctgaaagag ggggttatta aggcgtgaat acctacagca tcacattacc
     2101 ctggcctccg agcaataacc gctattaccg ccataatcgc gggcgcacac acatcagcgc
     2161 agaagggcag gcataccgcg ataacgtcac ccgaatcatt aaaaacgcaa tgctggatat
     2221 cggcctggct atgccagtga aaatccgtat tgagtgccac atgccggatc gccgtcgccg
     2281 tgacctggat aatctgcaaa aagccgcttt tgacgcactc accaaagcag gtttctggct
     2341 ggatgatgct caggtcgttg attaccgcgt tgtgaagatg cctgttacca aaggtgggag
     2401 gctggaactg accatcaccg aaatggggaa tgaatgatgt ttgagtttta tatggcagaa
     2461 cttcttcgcc accgctgggg gcatctgcgc ttatatcgtt tccccggttc tgttttgacc
     2521 gattaccgaa tactgaagaa ttacgccaaa accctgacag gagcaggagt atgaagtcag
     2581 agataacaat caactaatac tgttttgttg atttttgctt gtaattggcg ttctggtctg
     2641 atttttgtgg agtaagttga tgcgtgatat tcagatggtt cttgagcgtt ggggagcgtg
     2701 ggcggctaat aatcatgaag atgtgacctg gtcgtccatt gccgccggtt ttaagggatt
     2761 aattacttca aaagtaaaat ctcgcccgca atgttgtgac gatgacgcga tgattatttg
     2821 cgggtgcatg gcccgtctga aaaagaacaa cagcgatttg cacgatttat tagtagatta
     2881 ttatgtagtc ggtatgacat tcatgtcact ggcaggtaag cattgctgct ctgatggtta
     2941 tatcgggaaa aggttacaga aggctgaggg cataattgaa gggatgttaa tggcattaga
     3001 tatccggtta gagatggata tcgttgttaa taactctaat taatatgcca attgtttact
     3061 aaaaattatt aaaaatgggg cgttgcaacg cccccaaaaa taaagggtaa tatataacag
     3121 aaggtttata tagttagaag caaggttgtg ctcctaaagg aagtggcttg agggagccac
     3181 ttatatgttg gggaggcaaa gcctcccgca acatatcttt tagtaatcaa attagaactg
     3241 gtaaaccata cctacagcaa cgatatcatc ggtagcaacg ccagatgctt tcgtgaaatc
     3301 gctcttatca atcaggttga ttttgtaatc aacaaaagtg gacatatttt tgttgaagta
     3361 ataggttgca cctacatcaa tatattcaac caggtcctga tcaccccacg cacccaagtc
     3421 ttttccttta gattgcaggt aagcaacgga cggacgcaga ccgaagtcga actgatattg
     3481 tgcaactact tcgaagtttt gtgctttgtt ggcaatatgg ttattaccaa aaacagtcat
     3541 gttctgggtt tcagaatagg tggtagccag atagatgttg ttcgcatcat atttcagacc
     3601 agctgcccat acttcagcat tttgaccaga tgcattcagg ctgttgttac cgtagataac
     3661 ctgattatta gtgcggtcag atttagcata ggttgcacct acaccgaatc cttcatactc
     3721 ataagtagtg gagaaaccga aaccatcacc attagcttca gttacgtcag tgcggtcatt
     3781 tttaccctga tactgagcag caaagttcag accatcaacc agaccaaaga agtcgttgtt
     3841 acgataagtt gcaacaccag tggtgcgacc agtcatgaac acatctgttt gagtccaagt
     3901 gtcaccaccg aattctggca ggacgtcagt ccacgcaccg atgtcgtatg ctacaccgta
     3961 gttacggccg taatcgatgg agccgtagtc accgaatttc aggccagcga aggcaagacg
     4021 ggttttatct ttggaggaac cttgagattc agcgcggttg cctttgaatt catattccca
     4081 ctgaccgaaa ccagtcagtt gatcgttgat ttgggtttca cctttgaagc caagacgggc
     4141 ataagtagta tcaccatcat ctgcatcatt agaggagaag tagtgcttag cattaacttt
     4201 cccgtacaga tccagcttgt tactgtcttt attataaatt tcagctgcct gagcagacat
     4261 cgccatcagt actgatgcag ctacagcaga aattgccact gttaattttt tcatcgtgag
     4321 cccttttttt gaactattat taaaaaaatg atgtcattgc gcgataaata ttcatctaat
     4381 caatgtgatt atttcaagat gtaagttttg gtttctcgtt tgatttgtga agtagatctc
     4441 tatttttatc tgaacttttt tctatcgaat cctattcatg gctcttgact gaataaaaat
     4501 aaatctatta gccaatttat attaacggct gttatttata agtgctctat aatttgaagg
     4561 ttcaatttaa accggctaaa aataacactg gaaattattt gttggttatt tgttgggatt
     4621 tgcttatgta tttgtagtgg tgttttcaat actcggtagc attctcgcaa atatcattta
     4681 gtggtttacg tacgtaaaaa a
//
LOCUS       DAJTXQ010000211         4597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000211 DAJTXQ010000000
VERSION     DAJTXQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.214"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2152
                     /locus_tag="OO515_003987"
     CDS             <1..2152
                     /locus_tag="OO515_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6577774.1"
                     /translation="AWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGME
                     RFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMA
                     GGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPY
                     VGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQ
                     TDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDT
                     LNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAA
                     ANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGD
                     TPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDC
                     WDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKI
                     LDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVS
                     ELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAE
                     EYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDA
                     PVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2386..3669)
                     /locus_tag="OO515_003988"
     CDS             complement(2386..3669)
                     /locus_tag="OO515_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6577775.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3804..4496)
                     /locus_tag="OO515_003989"
     CDS             complement(3804..4496)
                     /locus_tag="OO515_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6577776.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1089 a         1140 c         1192 g         1176 t
ORIGIN      
        1 tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac ttaaaattaa
       61 gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga ccgctaaagc
      121 gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata actcactctg
      181 cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg cagctcagcg
      241 ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata tgcgtgagat
      301 gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt acggtgcatt
      361 agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc ttaatgacac
      421 ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac ctgcgccgta
      481 tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa acggttacgt
      541 caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta ccgatttccg
      601 taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct ggcaaactga
      661 tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact gccagcttaa
      721 ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg ccatcaaacc
      781 aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac tgaaccagaa
      841 cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc acggtaaagc
      901 caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc aggggattat
      961 cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg cactgcgcgg
     1021 tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac agccggtgtt
     1081 tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg caatcatcag
     1141 aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca acggtttgag
     1201 tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag ctaatgggca
     1261 gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg caaacggtgg
     1321 ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt acgccttcga
     1381 tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa ctcagcaacc
     1441 gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga ctgacaatat
     1501 cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg aactgattcc
     1561 ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt ttaccctgtc
     1621 tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg aaaatcagcg
     1681 tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc agattgctgg
     1741 tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg cggtgaaacc
     1801 aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg gcggtctggc
     1861 gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca actgggggat
     1921 gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca tttacatccc
     1981 tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg tgatccatga
     2041 agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg aagagcgcga
     2101 gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt aaaaagcgcc
     2161 atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg catcttatcc
     2221 gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc atccggcact
     2281 tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc gaaccgtagg
     2341 ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact tcttcgcctc
     2401 tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt
     2461 gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa
     2521 cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat
     2581 ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca gcgagcggcc
     2641 cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga ggaaaccgta
     2701 gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag tacgtgagtt
     2761 caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac
     2821 atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg tttccagacg
     2881 gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt
     2941 gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt tacctgcatc
     3001 tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc cattgttttg
     3061 tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc tctggcagct
     3121 atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg
     3181 acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca catcaatcgg
     3241 tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat ataccgttcc
     3301 ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct tgataattgc
     3361 cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg ccgggccgac
     3421 aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg cagcacctgg
     3481 tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat
     3541 cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gcggggttga
     3601 gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa
     3661 tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt
     3721 tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca acgaacaggt
     3781 cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg
     3841 tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg ttgagcaaac
     3901 ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta
     3961 ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat
     4021 actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga
     4081 atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat
     4141 gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag atatccatct
     4201 acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt aacaacagcc
     4261 agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata aattttcagg
     4321 tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg agtggcagcg
     4381 accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc gctcagtgtt
     4441 gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt gagcattgcc
     4501 gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct ccttattgct
     4561 ttaattttgc tcatgtaatt tatgagtgtc ttctgct
//
LOCUS       DAJTXQ010000212         4454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000212 DAJTXQ010000000
VERSION     DAJTXQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.215"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1327
                     /locus_tag="OO515_003990"
     CDS             <1..1327
                     /locus_tag="OO515_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6577777.1"
                     /translation="QQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAVHLMLGVA
                     ASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDRAVDDHR
                     HQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFYQRLAQ
                     DLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKAFSYL
                     QHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQLVH
                     KYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIAVR
                     QLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQ
                     IRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            1328..2944
                     /locus_tag="OO515_003991"
     CDS             1328..2944
                     /locus_tag="OO515_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6577778.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3000..3152)
                     /locus_tag="OO515_003992"
                     /pseudo
     CDS             complement(3000..3152)
                     /locus_tag="OO515_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3175..3552)
                     /locus_tag="OO515_003993"
     CDS             complement(3175..3552)
                     /locus_tag="OO515_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577779.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(3698..>4454)
                     /locus_tag="OO515_003994"
     CDS             complement(3698..>4454)
                     /locus_tag="OO515_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR6577780.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     IMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
BASE COUNT         1222 a          951 c         1100 g         1181 t
ORIGIN      
        1 tcagcagctt atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga
       61 gcgccgagac gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat
      121 gctaggagtc gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg
      181 cgttgctctt cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc
      241 cgctttgcca tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga
      301 tcaccgacat caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga
      361 ctccctatct caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg
      421 agcgcaagag ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca
      481 ggatctaggg ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag
      541 gcagactttt agtgatgagg cactagagaa actggattta aagttggcag agaacaagga
      601 cacgtgttgg ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca
      661 tagtattgtg tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc
      721 aagtgctctt actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa
      781 ctctgaagat ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg
      841 aattaaggcg gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca
      901 tgacagggat tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc
      961 ccctagagtt gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat
     1021 aaagcgtcta gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca
     1081 aactcctaac ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg
     1141 cttaaagcgt tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt
     1201 tattaagctt aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac
     1261 gttatctaaa gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga
     1321 agagtgagtg gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca
     1381 tttattccgt aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca
     1441 agaacggaaa tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa
     1501 cagtacaaca aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct
     1561 tcaacgagcc aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa
     1621 ggatggagcg cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta
     1681 tcacatccca cagttagagc tagctcggtc attattttta attaactcct atttctgtcg
     1741 aagctgtttg agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg
     1801 agatcattta gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagca
     1861 gtcggccgta gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta
     1921 tgaaagtatt tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg
     1981 gtgctttagc tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc
     2041 ttctaacgag gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat
     2101 gcttcctagc acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg
     2161 tagtacgcag cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt
     2221 ggacgatgag gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac
     2281 atggataagt tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag
     2341 ccaaactatt ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac
     2401 tgatgagcca cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc
     2461 aaccaattac aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat
     2521 tctaaaaact aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt
     2581 tgggcgtagc cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg
     2641 ggtgcaacgt aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa
     2701 aatgctttct accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga
     2761 aaagatacgg cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca
     2821 attatatgga caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca
     2881 agtatcgaga gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca
     2941 ttaaaggaat gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt
     3001 taaaggtggc catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc
     3061 cagttgcgga tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc
     3121 ttcatcgctg attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa
     3181 atagcctccg tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt
     3241 cttgagcgcg gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag
     3301 gcgagcacgt gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag
     3361 cgtgtcagga aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc
     3421 aaaggacgaa gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg
     3481 tacgacttca tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt
     3541 ggcggcagac acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg
     3601 gagcattagt cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat
     3661 cttgggcatt gatatttgaa acttcttgga tttgccccta tatttccaga catctgttat
     3721 cacttaaccc attacaagcc cgctgccgca gatattcccg tggcgagcga taacccagcg
     3781 cactatgcgg atgccattcg ttataatgct cgaacgcctc tgcaaggttc tttgctgccg
     3841 ttaacccgtc tggtttgggc atgatactga tgtagtcacg ctttatcgtt ttcacgaagc
     3901 tctctgctat tccgttactc tccggactcc gcaccgccgt gttcttcggt tcaagtccca
     3961 acatccgggc gaactggcgt gtttcattag cccggtagca tgaaccatta tccgtcagcc
     4021 actccactgg agacgacgga agatcgttgc cgaagcggcg ttccaccgct cccagcatga
     4081 cgtcctgtac tgtttcactg ttgaagccgc cggtagtcac cgcccagtgc agtgcctcac
     4141 gatcacagca gtccagcgcg aacgtgacac gcagtctctc tccgttatca cagcagaact
     4201 cgaacccgtc agagcaccat cgctgattgc tttctttcac ggccactctg cctgtatgtg
     4261 cccgtttcga tggcggtaca gcaggttttc gctcaagcaa cagcgcattc tggcgcatga
     4321 tccggtaaac acgtttggca ttgatcgcag gcataccatc aagttctgcc tgtctgcgaa
     4381 gcagcgccca tacccgacga taaccatacg tgggcagctc tccgataaca tggtgtatac
     4441 ggagaagcac atcc
//
LOCUS       DAJTXQ010000213         4410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000213 DAJTXQ010000000
VERSION     DAJTXQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.216"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(21..1958)
                     /locus_tag="OO515_003995"
     CDS             complement(21..1958)
                     /locus_tag="OO515_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6577781.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2369..3076
                     /locus_tag="OO515_003996"
     CDS             2369..3076
                     /locus_tag="OO515_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6577782.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="OO515_003997"
     tRNA            3182..3257
                     /locus_tag="OO515_003997"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4410
                     /locus_tag="OO515_003998"
     CDS             3455..>4410
                     /locus_tag="OO515_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6577783.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1058 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatcag
//
LOCUS       DAJTXQ010000214         4256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000214 DAJTXQ010000000
VERSION     DAJTXQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.217"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO515_003999"
     CDS             complement(<1..202)
                     /locus_tag="OO515_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6577784.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRR"
     gene            complement(417..1877)
                     /locus_tag="OO515_004000"
     CDS             complement(417..1877)
                     /locus_tag="OO515_004000"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6577785.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(1958..3142)
                     /gene="uxuA"
                     /locus_tag="OO515_004001"
     CDS             complement(1958..3142)
                     /gene="uxuA"
                     /locus_tag="OO515_004001"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6577786.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            3482..>4256
                     /locus_tag="OO515_004002"
     CDS             3482..>4256
                     /locus_tag="OO515_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCR6577787.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEV"
BASE COUNT          916 a         1166 c         1146 g         1028 t
ORIGIN      
        1 cccggcgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca cgtaccaccg
       61 tcctcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt tcgccaggat
      121 tgtatggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg taaggtcttt
      181 gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat caaatttttg
      241 ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg cacccgttaa
      301 tctcaaacta taccattagc actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa
      361 ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc agttagttac
      421 agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc cgccgtcacc
      481 gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaatgccgct caggcccagc
      541 agcgctttca cgcggtctgc gccctgatac tgcgcgttaa tcttctggaa ctcggccagc
      601 atcgggtcaa ccacgtcaat ggcattaccc tgttcatcca cgccctgggt gtagcgcatc
      661 cagccagcca cgcccagtgc caggtgacgc cagctgccgc cgttttgcag gtgcagacgc
      721 accggatcca gaagacgctg cggtaacttc tggctgccgt ccattgcaat ctgccaggta
      781 cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg
      841 tctgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag ggcaaaggcc
      901 gctttgcgat aatccggatt agtcatggtg tcggcaatgg tttcatagcc gccgaggtaa
      961 ccgaggtacg ccaggaaaga gtggctgccg ttcagcatac gcagtttcat catttcgaac
     1021 ggcacaacgt ctgcaacgaa ctgtgcgccc actttatccc agtccggacg accattaacg
     1081 aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca cgggtcgtaa
     1141 acacccagct ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg aacgatgcgg
     1201 tcaaccatgg tgcacgggaa ggtgacgttc tcttcaatcc atgccgccag ctgcggatca
     1261 cgtgcctgag ccagccccag aaccgcgacc ttcgccacat gaccgttttc acgcacgttg
     1321 tcgcaggaca tcaccgtaaa cgctttcagc cctttttcac gacgcagacg caaggcttcg
     1381 acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg cttgatcagc
     1441 gggttattga gatccagctg accgcttgcc gcatcggcgc aataaccttt ctctgtgacc
     1501 gtcagggaga caatcgccgt ctgcggacgc gccatcgcgt tgagaatacc ttcacaaccg
     1561 tcgatttccg gatgcagggc ttctttcatc gaaccgataa ttttcagctc ggtgctttct
     1621 gcgccttttt ccgccacggt gtacagcagt tgctgttttt tcaggttttc aatcagcaca
     1681 cggtcgttgc ctggcatcag gttaacttcg cagatacccc agtcgctgtc ggtactttcc
     1741 agcagatgat gggtgtacag cgcctggtgc gcgcggtgaa acgccccgca accgagatgc
     1801 acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg cagattgctg
     1861 tcaacaatag tagtcatcgt aaactccaga atccgtcttc agagagtgac ggcaaggaag
     1921 aaggaaccgg cacggcgcgc agccatgccg gtgggtatca acggctaaag aaagcgcgct
     1981 ggatcgccag ttcgacaccg cgaacttcag ccaggccttt cagacgacca attgcggagt
     2041 aacctgggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca
     2101 tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca acaatcgctt
     2161 tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg
     2221 ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga ccgaactgct
     2281 tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaacctgtg cacatggtga
     2341 aaccgttcgc catgctgttt acggtatcaa ccatccactg catatcttca atggtggaaa
     2401 caatgcgcgg caggccgagg atcgggcgcg gcggatcgtc cgggtgaaca gccatacgga
     2461 cgccaacttc ttcggcaacc ggaataatcg ctttcaggaa gacggcaaag ttttcgcgca
     2521 gtttggcttt gtcgatatct ttatacagct ccaggtgctt acggaactgg tcgagggtat
     2581 acccttcttc cgcgcccggc agaccggcaa tgatgttacg ggtcagacgc gctttgtctt
     2641 cgtcgctcat agtggcgaag cgttcagcgg cctgagcaat ttcttcttcg gtgtaatccg
     2701 cttccgcgcc tggacgcttc aggatatgca tttcgaatgc agcgaattcg atctggtcga
     2761 agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg gtccagtcga
     2821 gcaccggcat gaagttgtag cacacggtgc gaatgccgca ctgcaccagg ttgcgcagag
     2881 tctgctgata gttagcgatc cactgttcat agttgccagt gtgggttttg atatcttcgt
     2941 gaattggcac gctttctacg acagaccata ccaggcctgc gtcttcaacg atcgccttgc
     3001 gtttgaggat ctcttctacg gaccatactt cgccgttcgg gatatggtgc agcgcggtaa
     3061 ccacgccagt tgcgcccgcc tgacggacat cagctagaga aaccggatcg tttgggccgt
     3121 accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc cacagcaaag
     3181 agacgtggag ccccggagtt gtttaggtat ccggatgaac aaccagaccg cagccgtggc
     3241 gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt caacccgaaa
     3301 tctgaaattg gttaaccaca tcgcaagaat ttcactctca cggttgttgc tgcaaattgc
     3361 agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca acaatggttg
     3421 accaatttac ataacatgtc gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt
     3481 tatgcatgtg cttaacattc tctgggtggt attcggcatt ggcctgatgc tggtactgaa
     3541 tttgaagttc aaaatcaatt caatggtggc tttgctagtg gcggcgctgt ccgtcgggat
     3601 gctggcgggc atggatttga tgtcgctgct gcacacaatg aaagcgggct tcggcaacac
     3661 gctgggggaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga
     3721 ctccggcgcg gcgcaccaga tagcgcatac gctgctggcg cgtctcggtc tgcgctatgt
     3781 acagctgtcg gtaattatca tcggcctgat tttcggtctg gcgatgttct atgaagtggc
     3841 ctttatcatg ttagcgccgc tggttattgt cattgccgcc gaagctaaaa tcccgtttct
     3901 gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt tcccaccgca
     3961 gccgggtccg gtggcgctgg tgaatgctta tggcgcggat atggggatgg tttatatcta
     4021 tggcgtactg gtgacgatcc caagtgtcat ctgcgcaggt ctgatcctgc cgaagttcct
     4081 cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg tagatatgaa
     4141 taatctgccc tctttcggcg tttcgattct ggtgccgctg atcccggcga tcattatgat
     4201 ctctaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg aagtgg
//
LOCUS       DAJTXQ010000215         4201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000215 DAJTXQ010000000
VERSION     DAJTXQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.218"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1363)
                     /locus_tag="OO515_004003"
     CDS             complement(<1..1363)
                     /locus_tag="OO515_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6577788.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1428..1547)
                     /locus_tag="OO515_004004"
     CDS             complement(1428..1547)
                     /locus_tag="OO515_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6577789.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1702..1920)
                     /locus_tag="OO515_004005"
     CDS             complement(1702..1920)
                     /locus_tag="OO515_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577790.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2274..3752)
                     /locus_tag="OO515_004006"
     CDS             complement(2274..3752)
                     /locus_tag="OO515_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCR6577791.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1186 a          920 c          739 g         1356 t
ORIGIN      
        1 caactaaagc actgcccgtc tcctggttaa ccccatgtgc tataccgccg gtatagacaa
       61 tcagcgagcc tccggcgcta atattgctgc cagttgccgt accatctttc tcaacaacct
      121 gccggctccc ggtggaaata tttgtcgtgt cagctttccc gccgctcttg atattttgtg
      181 ttccgctgtt gatattggta cccgtggcaa taccatgatt attaatattc tgtgtaccac
      241 cattgattat ggtattggtc gcgtttctgg taacatccat taccccgccg ttatctattc
      301 gggtcgcatc agctttagca ttcgttaaaa ctgacattgt tcctttatct ttaataatcg
      361 tcttatttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
      421 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
      481 taccgttagt gtttgttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
      541 caccactaac atcaagcacg ccaccggaat aaacttcaat aacatccgag gagctggtgt
      601 aatcaacaat ttgcgtacca ccagaataga tctgagtatt ttttgccgtt gacttattat
      661 tcagagactg agttccacct tcaatcgtcg tgtccagcgc atggctttca tatactcttt
      721 gctcaccgcc atttttaatg gtggttgttc ctactgtgct ctgttcaaca tactgtcgtc
      781 caccatttat gattgtgttc gttgccatac ttccttgcgt gatgtcctga taaccggact
      841 tatttatcgt tgtaccatca acatgcccct gagttgttac tgtctgccct ccaccatcaa
      901 tgatggttcc attcgcagtc cccccactta tgtgtgaatt accaccctta attgtcgttc
      961 cattgctgat accgcctgta taaacgtcct gattgccact gtcgattatc gtaccggtgg
     1021 aaatgccccc gtcatgaatt gtctgtcttc ctccgttaat ggttgtatta ttagactgcc
     1081 ccatataatt gttattcctt ccaacatctt gatatccacc agattcaata agacttccat
     1141 tagatactcc gccataaaca ctctgctggc catggttgat aatatgagtg ttatttgttg
     1201 tacctcgttc atctactcgt tggttgccat ctacagtctc atcgtttacc acaccaataa
     1261 cattagtagt aaaggccgcc atcacgggcg gggcatatat caaggcagat atcaataagg
     1321 aaagtactga gcggcgacaa taatggggac tggtcctgtt cataaatttc atcctctgaa
     1381 aagtgaatac tgagtagcgt ttaagcgact ttagctctgc tgcaacatca gcccgcaggc
     1441 accagaccgg ggggttcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     1501 aaagacatca tgatgaaatg atgatgttcc acataagaaa gaggcatttt ttaaacacag
     1561 tgcgctgaag tgtgattgga taaaaaagtc aatctattca ggaaatacgg gcgtattctt
     1621 ttctttcgac aatcaggccg tcggcaaaat aaaatgattt acataatcgt ttctgatgaa
     1681 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattc ttttactttt
     1741 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     1801 accacgtttg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     1861 gtgcggttta cagactaccc gtgcgtccac cgccgataat gtcctgagca gttttttcag
     1921 tccggacctc ctgccacggt tttacgttga agaggtaacc ctgagcacgc agttcttcag
     1981 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag
     2041 ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattta
     2101 aactcagtaa atcacagcac agatcaaggg gttacgtgaa agcgtagccc ctttttcttt
     2161 ggtagtggca gcaaaatggt tgtagtgtaa aaaataatcc cgtttaatca atcaataata
     2221 catattgttt caatctacgt tattatctct ttgtaaaaat agccatttat taatcattga
     2281 aaactgcttt tagaacttga tacaacggga ctagtcacaa caggactatt ctcaacggga
     2341 tcatcctcag aggaactatc atcaaagtca tcatccataa ataaaatatc atcgaatggt
     2401 gccacgcccg tgatgagttt tattttatta ttacgatcag tcaagactcc acttaaaccg
     2461 ttttcgctca caggttttaa tgatttttca ttactcttgt tgtaagcagg cgcattaaaa
     2521 atacacggag tatcaagatc aaacaatgac gttctccagt tcacatattg aatatcatag
     2581 ttactgaagt tctgtccaga aaagaagcat cccttaaaat ccaatccacc taaattatat
     2641 aaaccatcct cttctttttg gagagtaatg ttaattctgg ctatctcccg gacaaaactt
     2701 ccttttttgt atttgaatac cgtttcaaga ttttcaccag acagcttgac ttctgggaat
     2761 aatttaaaat taaaccctac atcattatta ttcaaagtag atgaacgaaa gatggaaaaa
     2821 gcttgtagtg ctgaattata gctttcgatt ttatcttgag gctgcgctct tggtaaaaac
     2881 ttatagcagc actcataaaa attgaataaa cgttctgaac aattcccatt tttatcaatt
     2941 aatacccccc ggaattcttt cacaaaggca tccgttattt tttgaatatc tatggtgtgt
     3001 tcctttgact ctgacaaaga tggagtctgt tgaagtgcat tgctcatctg gttaaggaac
     3061 atctgcattt ctttaaattt atctccatag tggcctttaa cttgctgaga gtttttagtt
     3121 acaggtgaaa ccttatctat ttggttgtca tataaattat gtggttccat acagacattt
     3181 ggttgtgttt ctaaaataac actacgtgta ctacctggct taaatgaatc aacatgaaaa
     3241 tcactttttc ttatattatc gaatagggtc tgttcatttc tcttagcgca ttcaataaac
     3301 tgatcaacat tatttttatt cgatgacagg tcagtttcag aaaccgcatt ttctttgttc
     3361 tccagcttta gtttaatgag aagatcttcc cagacctgct tgcttaaaca tactacgtca
     3421 ggctcaccat cagtaactaa ctcttcatta ttatcaaagt atacgttgaa tacttttaag
     3481 ttattttcat caacatcata tttaatacgt tttaattgct ctccagatcc cataatgaca
     3541 aaggcgttgc cttcatgata tatacattca gacatcattt tttgtaaagt ttcaagagca
     3601 ccgcgatacg tccctgaggc agcttcctta caaattaaat ttataatact ataagctagt
     3661 agctccatgg attcagaaga ttttctttca ttacaatttc cactatctat aaatgagcta
     3721 gtgttaaatt cgttgctttt actaactaac attgtctatt cctcagttaa tgtctacatg
     3781 gctattttta attttattat tgtttgtcac tataaaaaat cgctcatttg agacaattgc
     3841 tggcattaac agcttcatat gctatacatg gtacttttaa ttaaattagc acaagaatgt
     3901 taaatttaat aaacaaaaag ttatttcgct gtatgataaa aaccacccgt tataatttat
     3961 tagtgaaatt cgtttttcga gtgttagaaa tttatatcta aatagcgttg attaatgagc
     4021 aaagccagac tcctgtaacg ctcacaagac tcatctacct gcgacgggta ttctggtcag
     4081 tagtagatgt ttaaggcgtg gcagagacat ttcatcctta ctctacggca ttggcccgca
     4141 tacaaatcgt ggttgtgaca ctcacttatc atcagtgagc gaacagagaa tagttcagtg
     4201 a
//
LOCUS       DAJTXQ010000216         4192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000216 DAJTXQ010000000
VERSION     DAJTXQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.219"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO515_004007"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO515_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6577792.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OO515_004008"
     tRNA            complement(330..405)
                     /locus_tag="OO515_004008"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OO515_004009"
     tRNA            complement(412..486)
                     /locus_tag="OO515_004009"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OO515_004010"
     tRNA            complement(603..687)
                     /locus_tag="OO515_004010"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OO515_004011"
     tRNA            complement(696..771)
                     /locus_tag="OO515_004011"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OO515_004012"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OO515_004012"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6577793.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO515_004013"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO515_004013"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6577794.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO515_004014"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO515_004014"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6577795.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1041 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaaaagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJTXQ010000217         4076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000217 DAJTXQ010000000
VERSION     DAJTXQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.220"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..784
                     /locus_tag="OO515_004015"
     CDS             <1..784
                     /locus_tag="OO515_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6577796.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="OO515_004016"
                     /pseudo
     CDS             797..1757
                     /locus_tag="OO515_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OO515_004017"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="OO515_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6577797.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OO515_004018"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="OO515_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6577798.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAJTXQ010000218         4059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000218 DAJTXQ010000000
VERSION     DAJTXQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.221"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..1181
                     /locus_tag="OO515_004019"
                     /pseudo
     CDS             35..1181
                     /locus_tag="OO515_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1475..2716
                     /gene="agp"
                     /locus_tag="OO515_004020"
     CDS             1475..2716
                     /gene="agp"
                     /locus_tag="OO515_004020"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6577799.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2754..2981)
                     /locus_tag="OO515_004021"
     CDS             complement(2754..2981)
                     /locus_tag="OO515_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6577800.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="OO515_004022"
     CDS             complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="OO515_004022"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6577801.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3659..>4059
                     /locus_tag="OO515_004023"
     CDS             3659..>4059
                     /locus_tag="OO515_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577802.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         1145 a          865 c          954 g         1095 t
ORIGIN      
        1 cgtcattatt tattaaattt tatagacgct atatatgggt agtaatatac atggaattag
       61 ttgcactgca aataattatt tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa
      121 aaatcaaaca tattcaatca cgctttttga aaacacactg gtgtgtttta tgcggttata
      181 caatgaactc agacgtaaag taaatgaaga ggatactcca tgtctggaat gtgaatcact
      241 agaaaaagaa tttgaggaaa tgcagaatga taatgatcta tcattattta tgagaacatt
      301 gcgtactaat gatacacaaa tttattcagg ggtttcagga ggtattacat atactataca
      361 atatgttcaa aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat
      421 cacagatttt aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc
      481 gtgtgatgat attttttctg agtattgttt agatgatgaa aatatgagta tccagccaga
      541 acagataaat acgccgggaa tatctgattt agatactggt tttaaaagaa catgagtatt
      601 catgggcgct gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg
      661 ttgataaaca aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg
      721 taaagacgtt atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt
      781 ttttatcatg gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca
      841 aaaaatgcat actgcattta cccgaattta tatttaatga taacaaggca cgccttgttt
      901 tagacaccga cccagagcag aaggttaatg aggtgaaaaa tttcctcact gctctttcag
      961 atagcatttg ctcattattt attgtttaca gcaatgtggc atcaatctcc ttagggaatg
     1021 aatccttttc aacagatgac gaccttgagt atgggtattt aataaacact ggcaatcatt
     1081 atgacgttta cctccctcct gaactttttg ctcaggctta cgagttaaac aataaggaaa
     1141 ggaatgcgca aattgatttt ttaactcgtt atgcaattta atgacaaagg catgtgctaa
     1201 aaaccattgt tattagcctc acttctctta ttattggtac tattgcctct gtattagtaa
     1261 cgccgctgat attctgttta tccacaggtg caattatcag cggcgtacgc gaggcagggg
     1321 ctaatcaggc atagtttgcg tcaaaccttg cctgtttttg aagatgtata tagaaaaaca
     1381 ggcgttcaac aagccatttt gcgaacctgt tcccggaaaa aagtcatatt tctgtcacac
     1441 tctttaatga ttgataacaa aagaggtgcc aggaatgaac aaaacgctaa tcgccgcaac
     1501 tgtggcaggg atagttttac tcgcttcaaa tgctcaggca caaaccgtac cggaaggcta
     1561 tcagctacag caagtgctca tgatgagccg ccataactta cgtgcgccgc tggcgaacaa
     1621 tggcagtgtg ctggagcagt cgacgccgaa taaatggcca gaatgggacg tccccggtgg
     1681 gcaactcacc accaaaggtg gcgtgctcga agtgtatatg ggccattaca tgcgtgaatg
     1741 gctggcagag caggggatgg tgaaatcggg ggaatgcccg ccgccggaca ccgtttatgc
     1801 ctatgccaat agtctgcaac gtaccgttgc gaccgcacag ttctttatta ccggcgcatt
     1861 cccggggtgt gatattcctg tgcatcacca ggaaaaaatg ggcactatgg acccaacctt
     1921 taacccggtg atcaccgatg attccgccgc attcagtgaa caggcggtgg cggcaatgga
     1981 gaaagagctc agcaaactcc agcttaccga cagctaccag ctactggaaa aaatcgttaa
     2041 ctataaagat tcccctgcct gtaaagagaa acaacagtgt tcgctggtgg atggcaaaaa
     2101 tacctttagc gccaagtatc aacaagaacc aggtgtttcc gggccgctga aagtcggcaa
     2161 ctcgctggta gatgcgttta ctttgcaata ttacgaaggt tttccgatgg atcaggtggc
     2221 ctggggagaa atcaaatctg accagcagtg gaaggtgttg tcgaagctta aaaacggcta
     2281 ccaggacagc ctgtttacct caccggaagt ggcgcgcaat gttgcgaaac cgctggtcag
     2341 ttatatcgac aaagctctgg tcaccgatcg caccagcgca ccgaaaatta cagtgttggt
     2401 tgggcacgac tccaacattg cctctctgtt aacggcgctg gatttcaaac cgtatcagtt
     2461 gcatgaccag aacgaacgca cgccgattgg cggcaaaatc gttttccagc gttggcatga
     2521 cagcaaagcc aatcgcgatt tgatgaaaat tgaatatgtg tatcagagtg cggaacagtt
     2581 acgtaatgcc gatgcgttaa ccctgcaggc acctgctcag cgtgtgacgc tggaattaag
     2641 cggttgcccg atagatgcca acggtttctg cccgatggat aaatttgata gcgtgttgaa
     2701 tgaagcggtg aaataacaga aaactccccc gcgagaagcg ggggagtcgc tggttaaacg
     2761 tttttacgtt cgatggtctg ttcgccccaa aaaagcgaat ctttatcggc cttagcaaag
     2821 gctttgacta acacttcatc gctaccttct tcccaaatct tttctgccat tttttcgtcg
     2881 tacccggcga cttcgaaaat ggcctcggct atttccggcg acgtattgcg cagagatgcc
     2941 cattcaccga cgtgatgagc tttcgcttct tgagttggca tgcgtatcct cctgttgaag
     3001 attagccgtt aagtttaact gccagacctg cgacatattc cccttgataa cgagcaatag
     3061 acagttcttc ctggcttggc tggcgtgagc cgtcaccgcc tgcgatggtg gttgcgccgt
     3121 acggcgtacc gccgcgaacc tgtgaaacgt caaataattc ctgcgctgcg tagccaatgg
     3181 ggacaatcac catgccgtga tgcgcaaggg tcgtccaggt tgaagtaata gtttgttcct
     3241 gaccgccgcc agtaccggtg gaactaaaga cgctcgccag ttttccgtat agtgcgccgg
     3301 aagcccacag gccgcccgtc tggtcgagga aggtacgcat ttgaccggac atgttgccaa
     3361 agcgggtagg tgtaccaaaa ataatggcgt cgtaatcggc cagttcttgc ggggttgcaa
     3421 ccggtgcagt ttgcgtttta ccgcctgctt tttcaaataa ttgcggcggc atggtttccg
     3481 gtacacgctt aacgacaact tctgcgccat cgactttgct tgcaccctca gcgactgcgc
     3541 gtgccatcgt ttcaatatgt ccgtacatgg aataataaag cactagtatt cttgccttaa
     3601 cactcatatc attgttctcc tgttcaatga accacttaga taagcatagt ccgaagatat
     3661 gaagctgcta gtgacataca tcaggtggag tactaaagag caagactcag gagactcctt
     3721 aagacggcaa acaaacctca ttgatgcttt ctactctaag cacaaaaacg attattacct
     3781 tcttcctgct cacagatatg ttgataaggg taaatctggt tttcaccagc aacataagaa
     3841 tcaaggttct gacttcagac gtatgtttga aaatgttatg tctggggcta ttccagaagg
     3901 ttccttgatt gttgtagaga actttgaccg cttcagtagg gctgatatcg acacagctat
     3961 agatgatgtc aggcagatac tgaggaaggg ggtctctata ctgactctgg gtgatggtga
     4021 actctacgac aagtcagcct taacagaccc tgtgaagtt
//
LOCUS       DAJTXQ010000219         4048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000219 DAJTXQ010000000
VERSION     DAJTXQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.222"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..757
                     /gene="ghxP"
                     /locus_tag="OO515_004024"
                     /gene_synonym="yjcD"
     CDS             <1..757
                     /gene="ghxP"
                     /locus_tag="OO515_004024"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6577803.1"
                     /translation="KLKVPGGILLTIIGISIVGLIFDPNVHFSGVFAMPSLSDENGNS
                     LIGSLDIMGALNPVVLPSVLALVMTAVFDATGTIRAVAGQANLLDKDGQIIDGGKALT
                     TDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTGLTAITVGVLFLLILFLSPLSYLVP
                     GYATAPALMYVGLLMLSNVAKIDFADFVDAMAGLVTAVFIVLTCNIVTGIMIGFATLV
                     IGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            909..2558
                     /locus_tag="OO515_004025"
     CDS             909..2558
                     /locus_tag="OO515_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6577804.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2712..4003)
                     /locus_tag="OO515_004026"
                     /pseudo
     CDS             complement(2712..4003)
                     /locus_tag="OO515_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          940 a          899 c         1014 g         1195 t
ORIGIN      
        1 aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt
       61 gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat tgagcgatga
      121 aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct
      181 gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgtgc
      241 cgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg gtgggaaagc
      301 actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgctg ctccggcagc
      361 ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccggtt tgacggctat
      421 caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctctctt acctcgttcc
      481 ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga gcaacgtggc
      541 gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat
      601 cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg
      661 tcgtctggtt tccggcgaat ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc
      721 gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat tctgaaaacg
      781 ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc
      841 tgaaaaaatg agaattcagg cataacgcct ttctcagatc ataaaaaaac atcgcaaaca
      901 gggaacgcat ggaaatcttc ttcaccatac tgataatgac cctcgtggtc tcactgtccg
      961 gggtggtcac tcgtgtcatg ccctttcaga tcccgcttcc gcttatgcaa atcgccatcg
     1021 gtgcgctact ggcgtggccg acgtttggtt tgcatgtgga gtttgatcct gaactctttt
     1081 tagtcttgtt tatcccgccg ttgctgttcg ctgatggctg gaaaacgccg acccgtgaat
     1141 ttctcgaaca tggtagagag attttcggcc tcgcgctggc gctggtggtg gtcaccgtgg
     1201 tcggcattgg cttccttatt tactgggtgg tgccgggcat tccgctgatc cccgcctttg
     1261 cgctggcggc ggtactttct ccgaccgatg ctgtggcgct ctccgggatt gttggcgaag
     1321 ggcgcatccc gaaaaaaatc atgggcattt tgcagggcga agcgttgatg aatgacgcct
     1381 ccggcctggt gtcgttgaag tttgccgtgg cagtggcgat ggggacgatg atcttcaccg
     1441 tcggcggtgc gacggtcgaa tttatgaaag tagccattgg tggtattctt gccggttttg
     1501 tggtgagctg gctgtatggt cgctcgctgc gattcctcag ccgctggggc ggtgatgaac
     1561 ccgcgacgca gatcgttctg ctgttcttgc tgccattcgc ttcttatctg attgccgaac
     1621 atattggcgt ttccggcatc ctcgctgcgg ttgccgccgg gatgaccatc acccgctccg
     1681 gtgtgatgcg ccgtgcgccg ctggcaatgc gcctgcgcgc aaacagcacc tgggcgatgc
     1741 tggaatttgt ctttaacggc atggtgttcc tgctgttagg tctgcagctg ccgggtattc
     1801 tggagacgtc gctgatggcg gcagaaatcg accctaacgt cgaaatctgg atgctgttta
     1861 ccgatattat tctgatatat gcggcgctga tgctggtccg tttcggctgg ctgtggacga
     1921 tgaaaaagtt cagcaaccgc ttcctgaaga agaagccgat ggagtttggt tcgtggacca
     1981 cacgagaaat cctgatcgcg tctttcgccg gggtgcgtgg ggcgatcact ctggccggtg
     2041 tgctctctat cccgctgctc ttgccggatg gtaacgtctt cccggcgcgc tatgagctgg
     2101 tgttcctggc ggctggcgtc attctcttct cgctgtttgt cggcgtggtg atgttgccta
     2161 ttctgctaca acacattgaa gtcgcggacc attcgcaaca attgaaagaa gaacgtattg
     2221 cgcgagcggc aacggcagaa gtggcgattg tggcgatcca gaaaatggag gagcgtctgg
     2281 cggcggatac cgaagagaat atcgataacc agctgcttac ggaggtcagt tctcgcgtca
     2341 ttggtaacct gcgtcgtcgc gccgatggac gtaacgacgt tgaaagttcc gtgcaggaag
     2401 agaaccttga gcgtcgcttc cgtctggcgg cattgcgttc tgaacgtgct gaactgtacc
     2461 acctgcgcgc cacgcgggag atcagcaacg aaacgctgca aaaattactg cacgatctcg
     2521 atttgcttga agcgttgcta attgaggaaa atcagtaagc cgaaagcgac gtaaccaggc
     2581 ccgaggtaat ctgaaaaagg tgcaatcttt tgcaccttta acaggttagc tcgggctaac
     2641 attcatgatt ctaaaacaaa taagttatct ctcttgtaaa gtcaattcat tggctgttct
     2701 tcgacttaac ctcaatctgc gtgttgcata atatctgcag taatcttcat agcgactgca
     2761 ttaatattat cctcagtttg tgacatgcca gtatgagagg tacaatcttc ttcaaagaat
     2821 ttctgtagtg ttaccagggt atcactatca atggggatat tttcctcccc acggagtatg
     2881 gaaatgccca ttccctggac gctagcctca tcaaaattaa cccgatagag acgggtatta
     2941 aacatacagg cttttgttaa aatagaacag ttgaaatggc agttgcttaa attggcatta
     3001 ttaaaattaa cattagccat attagagtcg gtacatataa ccatttcgag gaatgtattt
     3061 gttagccacg tacaaaagag tttgctatca attaagttta tattctggag gattgctttg
     3121 ttcagattga cgcgagacag atcagcatgt tcaaagttaa ttaaatcgag tttagagaaa
     3181 ctaaggtcgg ctttaaataa attaacatat ggcgctatta ctttataaag atttgcctca
     3241 gccatgtacg ccatataaaa atttgaatag tcaagaaagg tatttttaat attggctcta
     3301 ttcattgctg cattgtagaa ttgaacatgc ataacggagc atgcatttag agttccgtcg
     3361 gccattttta tctgagcgaa atttgagtca tcgagaatac aattatcaaa aagtattttt
     3421 ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa atagcgtttt
     3481 gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc acgatacctt
     3541 tcaggctaag gtgtgaaaaa tcgtaaccac catttactgt ctgtggaatt tcgatagtat
     3601 tattatggac ggcgacatta tttataaaat ataccacctt aatggatact tccttttctt
     3661 tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca tataaagcta
     3721 tatctataaa atgtttatgt attttcctgt ccgtttcctt cgtgattttt cctgatggta
     3781 aactatcata aattcttttc attttctgaa tcaaacctat cactttttca tgatcatcca
     3841 tcttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat
     3901 tatatgattt tgttttcttt gtatgtgatg aacttgtgaa agagtggtta tcgttaatct
     3961 ggcaaagaga gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt
     4021 aacaagaaag aaaaggcata ttctctgc
//
LOCUS       DAJTXQ010000220         4029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000220 DAJTXQ010000000
VERSION     DAJTXQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.223"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1742)
                     /locus_tag="OO515_004027"
     CDS             complement(<1..1742)
                     /locus_tag="OO515_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6577805.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDG"
     gene            complement(1827..2162)
                     /locus_tag="OO515_004028"
     CDS             complement(1827..2162)
                     /locus_tag="OO515_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6577806.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2162..2518)
                     /locus_tag="OO515_004029"
     CDS             complement(2162..2518)
                     /locus_tag="OO515_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6577807.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2518..4014)
                     /locus_tag="OO515_004030"
     CDS             complement(2518..4014)
                     /locus_tag="OO515_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6577808.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          907 a         1232 c          893 g          997 t
ORIGIN      
        1 tacccatcac cacgtggcat ctgccatccg gtcatgtcat agacaaaatc atggaactgt
       61 ttgccccact cgtcgaactt atcattcaga ccaaatccac tgttcagtgc atcagccaca
      121 aaatttttaa caagccaggg atgtgctttt tcaaactcct gcgccatcat gcccagttca
      181 agcaatccac caatcagact gatctttccg aggcccttta atttcagccc tgtttttcct
      241 gccgccgttg tccattgatt ctgggcgact gttgccgcac gaatacttgc caccgccttc
      301 acaccaatat acagggcaga aaccgttgct attgttttta ctgcctgttc ccatcccccc
      361 attgctctga caacctgatc aacctcctgc cagacttttt tcacaaccgg accgaccgtt
      421 tcccagttat caattatgag atatgcacca ccgacgagaa gcgcaattaa tccctttgcc
      481 ggcgtcatat tcatcacgct acccatgatt tttgttatgc gtgtcaatgt gccaatcgcc
      541 acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg tttttacgac ctcaggattt
      601 tctctgacaa acgtgcgcac ttcttccaga aaaggcttca tctcttttat gccttcattg
      661 agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg cattgatctg gttctgcagc
      721 agcaacaact gattttctgt cgtcgcggca cgggcggcat attctttctg cattgagccg
      781 ccatactgct gcgcatccgc cacacgatta aagttggttc gcaacaaatc cagattcgtg
      841 agcagcggcg caatggcccc cagggattcc ttcccgaaca gcgctttcag cacagcagcc
      901 tgtttttctt tcggcacttt tgccagagaa tccagtacgt gcagcatggc cccgcgggcg
      961 tctttctgca tatccgccgc cagttttttc gggctaatgc gcaacgagcg gagcactttt
     1021 ttctgcgatt tggtggcaga atccctcgct gtcagcgaca acataaaatt ctttatcccc
     1081 gtcgcagcaa tttctgactc cacccccatt ccggcaatgg ttgcccccat tgcggcaatc
     1141 tctccggagg ccacacccgc aacactgcct aaagggccaa tacgggtcac aacatcagaa
     1201 atctttttcg cacttgcagg accggtatta ccaagatagt taatcttatc cgcaagccct
     1261 gccacctctc cctgtgtcag tttaaaggca gtgcgccact gcgccatcat ctgaccggat
     1321 tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg catcgtcagt aaactgcatc
     1381 agctcgtcac gggcgatacc agcctgacca cccgccgcca caatttcggc gataccttcc
     1441 gccgacatgg gcagttctgt tgacaggtcg cgcacctgct ccgtcattgc cttaaacgct
     1501 tccggcgtat ccagaccatc caccactttc cggacatccg ccatttttga ttcaagggca
     1561 atggcagatt tgaccgggag cgccagcgcc cccagtactg cggttccggc acccgctgcg
     1621 cccagtaaca ggctggcaaa ctccttttta aaacctttca gctggcgctg cattcccttc
     1681 agcggtgccg ataccttatc cacggcagtg atgatcgcct tcagctgaaa actgtcagcc
     1741 atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc agttctgtaa actgcgaaac
     1801 agccccccgt cgcagctcca gtggattcag tttccagaac caggcaacat tgtagagtcg
     1861 tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac gcaggatctg catgctggtc
     1921 ttaaaaatat ccagttttgc catctgcgcc gccgatgaac gcgggatccc cgccagcaac
     1981 gggatatatt tcagtgccac ctggctgtcc agtttaatac tgccttcacc ggaaataatg
     2041 aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct cgcgcaactc cagcacatgc
     2101 agcatttcgt tatgcgctgt caccggtttt tttaaaacta tttctgtaac gttcattgcc
     2161 attactgata ccctccctct tcgccgtgga attcaagatc tgccgtgcct tcttcggcat
     2221 tatgattagc ctctccgtgc agccatgccg ccgaaagcac atacaccatg ccgtttgcca
     2281 gctcagcggt aatcgtcatc tggtctgagg tggtaatttt gtcgaccgga aagtttttgg
     2341 gcactttaaa cgtgccctta acgtaaggtg accgccaggt ctccttgtaa tccacatccc
     2401 ctgccatgcc gacgatgtca tcccggacat tggtgttcat cggcacttca atgccacctg
     2461 tcagcgataa ctgctgacca tccactttaa aaaaacaggt tccggcgatc tttgccatta
     2521 tgctgactcc tctgaatact gaagacggaa ctggttaacc accgcaaaga cacgtaactg
     2581 attaacataa tccggcggga acagcgtatt cagtcggttc gggttatctg catcacgctc
     2641 aactatcaga tactgtttaa acagatcgta attttccaca ataccggcac gctccatctg
     2701 acgatatgtc gccagaagtt cccctttgat aacggcagga gtgacaatcg cctgccccgg
     2761 accaaaacgg gtaccatcat ttgccagctt gtgacgtccg tacttgcttg tgatgaccga
     2821 tttcagtttg cgaagaacat atgcactggt atgcagagtt tcactgtcca gatagctgtt
     2881 gtctgccacg ccatacgcat tctttttgta ggtggttacg gaacgctgga tctgcaacgt
     2941 gccgccttcc acataagccg tcgccacgcc gtgagataaa agggtctgct gctctgtcat
     3001 gatgaatcgc ttacctttcg gtgccggaag catccccacc agctcccccg tctgtgtcgg
     3061 acgggcagga tcattccgga taaataccgc ttcacgggca aggcgactgg caaccagttc
     3121 atcgacaggc gactgggttt ctttttcgta accggcaagc gtgatatgtt gctgattatg
     3181 catatctccg gcaccaacca gctctgacag cgttcccagt tttgcggtat agccatgccc
     3241 gtataactgg cgcgcataac tccagcgacc gctgctgtca ttcatttcgg tcatcatcat
     3301 attgatggag gcggcatcgt tgaacggcag accgataaag tcgaatgcct catcgcccat
     3361 agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg gcagcaccga cttccgtcac
     3421 cacccgaagc cctgctggca gaatctcacc accaccagaa ccataataat tcagacagac
     3481 cggtaactca ttaccataca gccccttatg acgggcagtc agcgtcacca cacctgcatc
     3541 agatgacgcc gtaaacggca gggtgataac cccgtttacc gcttccttaa tcgcggtggc
     3601 aaccgcagtg gcatcatcgc cattcaccac aggcacctgt acacgggagc gcccgacata
     3661 cagactcagg gtgccgcttt cctctgcttc tccggcaacc gtcaccctga ccgtcgccgc
     3721 cgcccctctg gcttccggta ctgcaataac atacagttca ccgaaaggat ctgtctgacg
     3781 gtagacttcc accatacgcg ccagctggct ccccgcccca caaatctggc gggcataatc
     3841 tgccgatggc atcagcacca ggctgttaac ctcaatggct gcatcgttgc tggcatgccc
     3901 gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc gcagaattgt tcatctcggc
     3961 gtagaacaac ggcaccagcg tattcgacgg aacagtatta aaacttatcg tcatgatttt
     4021 ttagcctta
//
LOCUS       DAJTXQ010000221         4000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000221 DAJTXQ010000000
VERSION     DAJTXQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.224"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OO515_004031"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OO515_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OO515_004032"
     CDS             1909..3261
                     /locus_tag="OO515_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCR6577809.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OO515_004033"
     CDS             3285..3767
                     /locus_tag="OO515_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6577810.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..>4000
                     /locus_tag="OO515_004034"
     CDS             3811..>4000
                     /locus_tag="OO515_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577811.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDV"
BASE COUNT         1073 a         1029 c         1070 g          828 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg
//
LOCUS       DAJTXQ010000222         3989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000222 DAJTXQ010000000
VERSION     DAJTXQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.225"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            338..946
                     /locus_tag="OO515_004035"
     CDS             338..946
                     /locus_tag="OO515_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6577812.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1044..2435
                     /gene="selA"
                     /locus_tag="OO515_004036"
     CDS             1044..2435
                     /gene="selA"
                     /locus_tag="OO515_004036"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6577813.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2432..>3989
                     /gene="selB"
                     /locus_tag="OO515_004037"
     CDS             2432..>3989
                     /gene="selB"
                     /locus_tag="OO515_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6577814.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTD"
BASE COUNT          885 a         1030 c         1178 g          896 t
ORIGIN      
        1 aacaggccac accggtgggg gcaccgatgc gcaccccggc tgaaccctga acaatgctac
       61 cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc tgctcatcct
      121 tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat aagaaagcct
      181 taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac catgataaat
      241 atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc ccatcaccag
      301 gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg gtagctacac
      361 cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa ctttcgaatt
      421 tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc cgttaggaaa
      481 agtgccggtg ctggtgaccg aagagggcga atgctggttt gattcgccga tcatcgctga
      541 atatattgag ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc tggagtcgtt
      601 gcgggtgcgc aaaattgagg cgctggcgga tggcattatg gatgccgggc tggtatcggt
      661 gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat gaattgttac gccagcggga
      721 gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca cactcaaaac
      781 cgatacggtc aatctggcga ctatcgccat tgcctgtgct gtcggatatc tcaatttccg
      841 ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg tcgaaaacct
      901 gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg cgatatgtcc
      961 tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc cgtgtgagag
     1021 ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat agtcaacttc
     1081 cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat acttatggtc
     1141 acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa gtgattcgtg
     1201 acagccagac gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat gcccggttga
     1261 cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc gtgctgcata
     1321 ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg caggctatgc
     1381 gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat cgcgatcggg
     1441 cgctggcgca actgctgtgc cgtattacgg gggcggaaga tgcctgtatc gtcaataaca
     1501 atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag gtggtagtat
     1561 ctcgcggcga attggtggag attggcggcg cgtttcgtat tcccgatgtt atgcgtcagg
     1621 caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat gattatcgtc
     1681 aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac tacagcattc
     1741 aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa gagctggatg
     1801 ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag tacggtttgc
     1861 cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg agcttctccg
     1921 gcgacaagtt gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa gagatgatcg
     1981 cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg accctcgcgg
     2041 cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa aaattaccga
     2101 ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa cgtttacagg
     2161 ctccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca tgtctttcgc
     2221 agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta acgtttacac
     2281 cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt gaattgccag
     2341 tgccgatgat tggtcgtatt tatgacggac gattgtggct ggatttacgc tgccttgaag
     2401 atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg gacacgttga
     2461 ccacggcaaa acaaccttat tgcaggtgat tactggcgta aatgctgacc gtctgccgga
     2521 agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc agccggatgg
     2581 tcgcgtgcct agttttatcg acgtacccgg tcatgaaaag tttctttcca acatgctggc
     2641 gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg gcgtgatggc
     2701 gcagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc tgacagtggc
     2761 gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac gccaggtaaa
     2821 ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg cagcaaccga
     2881 aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttaccaggac gtgaacacgc
     2941 cagccaacat agtgtccgcc tcgcgatcga ccgcgcattt accgtaaaag gtgccggtct
     3001 ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac tctggctgac
     3061 tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc caacagaaac
     3121 cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa aagagcagat
     3181 taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac gggtgattgt
     3241 cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc accacgccgc
     3301 cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc tggtgttcga
     3361 cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct cggcccgcaa
     3421 cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta aacgtaagcc
     3481 ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg atgcgttatc
     3541 tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc gccagctcaa
     3601 cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg gttatagctt
     3661 gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat tagcgactta
     3721 tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac gtatggcgtt
     3781 gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg aaagcggcga
     3841 catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct tcagcgaaga
     3901 gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt ggtgggtgcg
     3961 tgatctggca aaagagacgg gtaccgacg
//
LOCUS       DAJTXQ010000223         3988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000223 DAJTXQ010000000
VERSION     DAJTXQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.226"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO515_004038"
     CDS             complement(<1..242)
                     /locus_tag="OO515_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6577815.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLG"
     gene            complement(305..754)
                     /locus_tag="OO515_004039"
     CDS             complement(305..754)
                     /locus_tag="OO515_004039"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6577816.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(751..3843)
                     /gene="ebgA"
                     /locus_tag="OO515_004040"
                     /pseudo
     CDS             complement(751..3843)
                     /gene="ebgA"
                     /locus_tag="OO515_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT          927 a         1012 c         1069 g          980 t
ORIGIN      
        1 cccagcgaac tttttaccca tgcgtagaca ccggcttctg agtttttatt taacgaaaca
       61 aattctgcga tgatcagaca gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc
      121 ggtgccgagg ccagtccaag atcaatatta ttattgataa cgttgttaaa gctgtaaacg
      181 gcggcaaaag tcagcgagag caagccgaat ttgccgattg tattacgttt ggtatcagac
      241 ataacacatc tccgaaaatg agtagggtag cggtggcggc aaactccttt tgccgccgta
      301 gttgttattt gttatggaaa taaccatctt cgatggtgac tttgagaacc acttttttga
      361 ccgcgttatt gcagataaac cgatacgctt catggatatc gcaaataacg atttgccctt
      421 cgtggacctc aacggtttct ccgcagcctt ttaaatattc acggtcagtt tcatcacgat
      481 aatattccac tacctgtaat gtctctttcg gcgcatattc aattttttgc tgcccttgca
      541 ggtagtaatg cacttcaaaa tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt
      601 ctgtctccac gcggtaagtc aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt
      661 tgtcgatatt ttcaatcgct tcaacgcagc gttgccactt cttgccagag gcgtaaatct
      721 ggcggaactg ttctaagtta tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt
      781 ggaaaagaac cctgcgccga actcatacga cgccaggctt tgcgcggtag cttctccgcc
      841 agaaaccggc agcaacgtaa agccgtagct gaagtcacgg aaccagacgc gccaggagtc
      901 cagcacctcg ctgccccagg agttggagcc gaggccaagc agctggtgat cgaggttcag
      961 ggtgatgtca tcactgcgct gcagctcgtt acagtgctgg gcagcgtgga tgttttccta
     1021 ggtatagtgc caggcgctga agttaattgg gcgctgcgga accaccagca gaccgttacc
     1081 gtggcggtta gtcagtgccg tccagcggac atgctgacgg ttaccgttgt tctgcgggaa
     1141 gggatagttc tcgaacatgg catcgacggt gctgcgccag atatcgatga tgttagcctg
     1201 ctggctgtcg gcgtagtttt cgcccggtcc acgaccgtaa tacgccacct gatcgtattc
     1261 gccgttaatt cccatggtga aaccgatgca cggaatgatg tgcggatagt cgccgtaacg
     1321 ctcgccggaa agcgccacgt taacctggcc atcggcagcg atgcgccaga tgtaggtgca
     1381 gcgcattccg aaatcaaaca ccggcggggc aataaccgtg cggctgatga tcaacacttc
     1441 accctcgctc tgttctacgg caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt
     1501 cggctgccac aacccttcgt actcctgctt gtggttgtcg atcatcggct tgaagaagtt
     1561 gatctttggc tcgcgggtca gcagcgactc gccatttacc tgccaggatg tcggtttgcc
     1621 actcattttt gagaaggtga tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc
     1681 gtcttccagc gtcagcggac gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt
     1741 ttccttcagc gggaactgat aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg
     1801 ggaatctttg gtcaccgtaa tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt
     1861 gatctgcaag ggggcttcgc tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc
     1921 gagcgtttca ccttcggcgc gcacctctgc gtgcaggggg tagtcatcaa gcgtggtaaa
     1981 ccacagttta ttttcgactt tcaactcgcc gcgagtcaga tcccgcgcgt ggatttttac
     2041 cggcgcgata acctgtttgt actctttcag gcccggtccc ggcgtctgat cggaatagat
     2101 caaaccatca agacagaagt tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt
     2161 ataccagaca ttgccgtggt cgtcctgtgc ctggatcccg tggtcgcacc actcccagac
     2221 ataatgaccc tgaatgcaat cgtgcttata gaagacgttc tggtactccg tcagcccgcc
     2281 cggtccgttc cccatcgcat gagcatattc acagatgatg cgcggcttcg gatgcgggta
     2341 ttcaccaaac tcattcatca gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac
     2401 ttcagcatcg cgatcttctt cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc
     2461 gtggtacatc gcgcggatgt tacagccata gccggattca ttgcccagcg accagatgat
     2521 gatcgacgga tggtttttct gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt
     2581 ttcccactgc ggatcgtcgg taatacggct gatatcgccg acattagcaa agccgtgcga
     2641 ttcgacgtcg gtttccgcca tcacaaacag gccgtagata tcacacagtt cgtaaaaacg
     2701 cggatcgttc gggtagtgag cggtacgcac ggagttgatg ttgtgctgct tcatcaactg
     2761 gagatctttc tcgacgcgat ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg
     2821 acggttgacg ccgtgcagca tcacataacg gttattgatc cagaacagac cgtcgcgcac
     2881 tttgatatca cggaagccaa cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc
     2941 tttcagcgtc atgaccagat ggtaaagata aggggattct gctgaccatt gctgcggctg
     3001 ttcgacagta aaagcaaagc tggcgctggt cagtttttca attgccaaat gatcaatggc
     3061 gctgctgtgc accacgcgtt cgccatcaaa cagggtatat tccagcgtcg tgacgacagg
     3121 ggaggcggcg agattttcca gcaccacttc gcaggaaagc gtggcatcgc aataggcttc
     3181 gtcaaagtcg gtacgcacgg tgaaatcgtt aatatgtgtt agttgttttc cgatcagata
     3241 aacatcgcgg aagatccccg ctgaccacca catatcctgg tcttccacgt aggtagagtc
     3301 cgcccattgc atcacgcgca cacacaacag gttgtcgccg gttttaacca tcgcgctgat
     3361 gtcaaactct gcggtcaggc gactgccctt gctgaaaccc acatactgac cgttaacgta
     3421 gacttcaaaa taggtttcga cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc
     3481 gtcgctgagg gtgaaaatac gttgataggc accggttggg ttatcgctgg gaacaaacgg
     3541 cacatcgatg gggaacggaa aaccttcgtc ggtatattgc agtttgccgt gaccttccat
     3601 ttgccacatg gcggggacgg taatatgccc ccagtcagcc attaactcag aggtgaaggc
     3661 ttcaggcact tgcagcgggt ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag
     3721 aaacaggctg ctggtttcgc gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa
     3781 aaagtacgca cgtggcgcaa gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt
     3841 catagggcct cccggtagag agaaatcgga accattcatg gtgatggtag tttgtgtaat
     3901 tgctggggag tttagtaaaa tttttactaa aaagtgacag gtaaatgcat ttactttaac
     3961 tggatcacga aaataaacgg cagcactg
//
LOCUS       DAJTXQ010000224         3961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000224 DAJTXQ010000000
VERSION     DAJTXQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.227"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO515_004041"
     CDS             complement(<1..242)
                     /locus_tag="OO515_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6577817.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO515_004042"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO515_004042"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6577818.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OO515_004043"
     CDS             2255..3904
                     /locus_tag="OO515_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6577819.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAJTXQ010000225         3958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000225 DAJTXQ010000000
VERSION     DAJTXQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.228"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..451
                     /locus_tag="OO515_004044"
     CDS             116..451
                     /locus_tag="OO515_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6577820.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            444..926
                     /locus_tag="OO515_004045"
     CDS             444..926
                     /locus_tag="OO515_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577821.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(960..1040)
                     /locus_tag="OO515_004046"
     CDS             complement(960..1040)
                     /locus_tag="OO515_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577822.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1076..1500)
                     /locus_tag="OO515_004047"
                     /pseudo
     CDS             complement(1076..1500)
                     /locus_tag="OO515_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1930..2420
                     /locus_tag="OO515_004048"
                     /pseudo
     CDS             1930..2420
                     /locus_tag="OO515_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2645..3136
                     /locus_tag="OO515_004049"
     CDS             2645..3136
                     /locus_tag="OO515_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6577823.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3471..3929
                     /locus_tag="OO515_004050"
     CDS             3471..3929
                     /locus_tag="OO515_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6577824.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1475 a          635 c          642 g         1206 t
ORIGIN      
        1 acatacacac aatgccccac ctgccataaa agttaatgcg ggatacaaag agagcattgg
       61 tggcgcaaag aatttcaata acaaaatcct caaacatata aaaagtcatc taattatgtt
      121 gagcgcattt gtcataggcg catattctgc atattggcta tggaataata atcaaccgaa
      181 gccatttttc aaggattaca agactgttgc ggaaattaat ggctgccatt ttaatgtaac
      241 agaagataca attgatgggt tgaaagagtt cgataaatat aaaacacgga tactggattc
      301 ggggattaac tgtaaaaaat atccgtggtt atacttccct cttgcaaaat cctcaccggg
      361 gatgattgtt atggcatgca ataaaaacta taaccaacat gaagtggcag actgtttaac
      421 cctgtcttac cgtgaggtta atcgtgattg attataaaaa aaacttatta tttattttag
      481 tttttatcag tgggtttatt ctcttcactg tctattctta cactgcagaa aaaatgatat
      541 ataacgaaac atgcactgca aattgggtaa tttttaatga ccagggacga gcaaatctta
      601 ccatcgactt tatgtataac aaaaaaaata aaacaggaac tgttgcgttg agtggaacct
      661 ggcaacaagg taacagagaa agtaaatcaa tacggcgaaa tattgaatac acatggattg
      721 aaaactatga cacagcccat ttaacatcaa agaaagttaa taagtttgag attatggatc
      781 aagttgatga tgatagactt gcacaactta ttcctgattt ttatgtcttt ccagaaaaaa
      841 gtgtaagcta taatatacta aagcaaggta agcatgcttt tattttgagc attggtaaca
      901 gagcaataat gcattgtgca aggtaaacta aaatagataa caaaaaagat ggaacctcgt
      961 taatcataaa acaataaaat taaatattct cgcagtatat ggcagtctaa agcatcaaag
     1021 ttttgatcaa catctttcat tttagacatc tccttgcaat gtaaaatatc atgtatcaac
     1081 aacatctggt gcaaatccat gagtcgaact cgacattcat cttagctcga ttattattcc
     1141 ttcgtacagt cgatgtaaac aatacagaaa gaggattatt aagaaccagt ttactcagga
     1201 atcaatagag gaaaggacta acgtttcttt aaaagaattg atttcatcat ctgttaaact
     1261 aaactcatca ttgacagatc gtgagatata actgttttta actttactct ttacgttgac
     1321 tttattgaca gaattaacat tcacatatct tgaatttaat gtccatgatg ttgtttgcgt
     1381 gagaacattc tcagcattaa ggaatttttt taacggaatc cttggtttct ttttcgagtc
     1441 cgaatttaca atatcatgca tatagaccat ttcatgaatc tgcgaaaact caatgtccat
     1501 tgtatacctc acatttttac cgtgactcga tgttactgtt caataatcac cttccatcaa
     1561 tactaaaatt aataccccta atgtgccgat aacaaatata gtcattctac gtaacgtctc
     1621 cataaggtga taaccttgat caaatgaatt tcgaatataa taaaggaaat cttttgagat
     1681 gtttatcacg aataaagcta tttatgttac caatatcatt tcgagtgcgc acactatcta
     1741 tctgtttttt cgagatataa aaaacaacgt gtaagttatg taaattgccg atgctactga
     1801 gtaacattat ggtaaaaaaa gtaaaaatga gcgatttttg atagaataaa gcatgagaaa
     1861 aggtgatatt tgacattatc agaagctgcg aattcggatt ttgctctaat caaatgagag
     1921 aaatgtaaaa tgaagccacg aaatattaat aatagcctac cactgcaacc attagttcct
     1981 gatcaggaga acaaaaataa gaaaaatgaa gagaaatccg ttaatccagt taaaatcaca
     2041 atggggtctg gtttaaatta tattgaacaa gaatctcttg gaggaaaata tctaacacat
     2101 gatttgtcaa taaagatagc ggatatttct gaagagataa ttcagcaagc aatattatct
     2161 gctatgagca tatataaatt ttcgataaca gatgatttaa tgagtatggc tgtaaatgaa
     2221 ctcataaaac tgaccaaaat agagaataat gtagacctga ataaattcac tactatatgc
     2281 acagacgttc tatcccccct cgtcaccaga cataataaag aaaaaaaaca aacgacattc
     2341 tacccttctc aaaaatcccc tttttaattt tcattgaaaa aatggcaatt gatactatga
     2401 atccaggtca tacacattaa ggcatttatg taatgtcgta attaagataa ctaataaggt
     2461 gaatattagt aaaacaacta atgatgttat tagtttgtag tgaacagtac ttttaccaat
     2521 aatgaaaaat ataccataag caatgcaagc ttaaaactaa taatttaaaa aagataaatg
     2581 taaaaatatt aatgagtcaa tcaataataa tttagcatca cgagacacat cacagaggaa
     2641 tattatgagc acagaaacaa ttgaaatatt caataatagt gatgaatggg caaatcaact
     2701 aaaacacgca ttatcgaaag gagaaaatct ggcattacta catggtttaa ctcctgatat
     2761 ccttgataga atatatgcat atgcattcga ctaccatgaa aagggtaata taacggacgc
     2821 agaaatttat tataaatttt tgtgcattta tgcgttcgaa aatcatgagt atctaaaaga
     2881 ttttgcatca gtatgtcagc caaaaaagaa atatcaacaa gcatatgacc tttacaaact
     2941 aagttacaat tactccccgt atgatgacta ttcagttatt tatcgtatgg gtcaatgtca
     3001 gattggggct aaaaatatcg ataacgcaat gcaatgtttc tatcacatta ttaacaattg
     3061 tgaggatgat agtgttaaga gtaaagcgca ggcatatatt gaactcttaa acgataattc
     3121 agaagataat ggctaagcat tacaatcatt tggttaaatt tgctggttat agtgcagtta
     3181 actactataa ccagcacctc ttatgttttc ttaaatcagc atcttaaaac agttaagagc
     3241 atactataaa tcattatttt tcaatgcgtt agagaacaat ctactttata aaaattgaat
     3301 aaggctattt cagattacat cactgaattc ctaagataaa ttagtgaaca caaccataga
     3361 tatgtcgaat gtattttcct gtagcattaa tgacacataa atatgaatag ccataatttc
     3421 tattgcttaa gcaggatgca agaaaccaat tttttcatag aggttaacta atggacttag
     3481 aaaataaatt ctcatatcat tttcttgagg gattaacgct cacggaagat ggaattctta
     3541 ctcaaggaaa tgagcaagtt tatattccac agaaagagtt aggtgtatta atagtattac
     3601 ttgaatccgc tggtcatgtt gtactgaaag atatgatcat cgaatcagta tggaaaaata
     3661 ttattgttag tgacgagtcc ctgacaagat gtatctattc tttgcgctgc atttttgaaa
     3721 aaattggcta tgatcgttgc atagaaacaa tctaccggaa aggttatcgt ttcagcgggc
     3781 aggttttcaa aactaaaatt aatgaagata atacttcaga ctattccata gctatattcc
     3841 ctttcactac ttcattgaat acactggatc cattaatact taatcaggaa ttagtgcaaa
     3901 tcatttcaaa taaaaaaatc gatggttaga ctggccccct gaatctccag acaaccag
//
LOCUS       DAJTXQ010000226         3954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000226 DAJTXQ010000000
VERSION     DAJTXQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.229"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..327
                     /locus_tag="OO515_004051"
     CDS             <1..327
                     /locus_tag="OO515_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6577825.1"
                     /translation="TFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYY
                     SAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKE
                     NVAPGG"
     gene            554..1819
                     /locus_tag="OO515_004052"
     CDS             554..1819
                     /locus_tag="OO515_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6577826.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1831..2697
                     /gene="ydiB"
                     /locus_tag="OO515_004053"
     CDS             1831..2697
                     /gene="ydiB"
                     /locus_tag="OO515_004053"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6577827.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2728..3486
                     /gene="aroD"
                     /locus_tag="OO515_004054"
     CDS             2728..3486
                     /gene="aroD"
                     /locus_tag="OO515_004054"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6577828.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3630..>3954
                     /locus_tag="OO515_004055"
     CDS             3630..>3954
                     /locus_tag="OO515_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577829.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQ"
BASE COUNT         1020 a          883 c          958 g         1093 t
ORIGIN      
        1 acattttatg tggtgataat atttgctttt gtcattggtt ttacctctgc tggaggtgtt
       61 gtgcaaattg gcctgacgtt aatggctgaa cgtttccctt acgctaaagg taaagctaca
      121 gggatctatt acagtgcggg cagtattgcg acctttacta ttccgttgat tacggctcat
      181 ctgtcccaaa gaagtattgc cgatattatg tggttcgata ccgccatcgc tgccatcggt
      241 tttttactgg cactgtttat cggcttacgc agccgcaaaa aaacgcggca tcactcgcta
      301 aaggaaaatg tcgctccggg tgggtaatgc aatattcttt tcaggtcatg caagatctta
      361 cggataaata actctttctg cgctaactaa ggaaaatcgc gatcaaaaac aaactatgac
      421 atgcaatatt cctggaaaca taaactttat gccatgtacc cagggaaaat catcttcagt
      481 atagtaatta tgtaaaccgt cggagaacaa tacgtacggt aacgaaatta tctttcagca
      541 aggagctgtg aaaatgtctc aaaataaggc tttcagcacg ccatttatcc tggctgttct
      601 ttgtatttac ttcagctatt tcctgcacgg cattagtgtt attacgcttg cccaaaatat
      661 gacatctctg gcagaaaagt tttccactga caacgcgggc attgcctatt taatttccgg
      721 tatcggtttg gggcgattga tcagtatttt attcttcggt gtgatctccg ataagtttgg
      781 tcgtcgggcg gtgatattaa tggcagtaat aatgtatctg ctattcttct ttggtattcc
      841 cgcttgcccg aatttaactc tcgcctacgg tctggcagtg tgcgtaggta tcgctaactc
      901 agcgctggat acgggtggct accccgcgct catggaatgc tttccgaaag cctctggttc
      961 ggcggtcata ctggttaaag cgatggtgtc atttgggcaa atgttctacc caatgctggt
     1021 gagctatatg ctgctcaata atatctggta cggctatggg ctgattattc cgggtattct
     1081 gtttgtactg atcacgctga tgctgttgaa aagcaaattc cccagccagt tggtggacgc
     1141 cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg ttagtctggc tggaaggtgt
     1201 ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt tatgtgattg tggtgtggat
     1261 gcccaaatat gcgatggctt ttgctggtat gtcagaagct gaggcattaa aaaccatctc
     1321 ttattacagt atgggctcgt tggtctgtgt ctttattttt gccgcactac tgaaaaaaat
     1381 ggtccggccc atctgggcta atgtatttaa ctctgcgctg gcaacaataa cagctgccat
     1441 tatctacctg tacccttctc cactggtgtg taatgccggc gcctttgtta tcggtttctc
     1501 agcagctggc ggcattttac agctcggtgt ttcggtcatg tcagagtttt tccccaaaag
     1561 caaagccaaa gtcaccagta tttatatgat gatgggtgga ctggctaact ttgttattcc
     1621 gctgattacc ggttatctgt cgaacatcgg cctgcaatat atcattgttc tcgattttac
     1681 tttcgcgctg ctggccctga ttaccgcaat tattgttttt agccgctatt accgcgtttt
     1741 tattattcct gagaatgatg tgcggtttgg cgaacgcaaa tttagcaccc ggttaaacac
     1801 aattaagcat agaggttaaa ggagttaatt atggatgtta ccgcaaaata cgaattgatt
     1861 gggttgatgg cttatcctat ccgccacagt ttatcgcctg aaatgcagaa taaagcctta
     1921 gaaaaagcgg gattgccatt tacctatatg gccttcgaag tggataacga tagctttccc
     1981 ggagcaattg aaggattaaa agccctcaaa atgcgcggaa ctggtgtgtc gatgccgaac
     2041 aaacaactgg cgtgtgaata tgttgatgaa ttaacgccag cagccaaact ggtgggtgcc
     2101 atcaacacca tcgttaatga tgatggctat ctgcgtggct ataacaccga cggcacgggc
     2161 catattcgcg ccattaaaga gagcggtttt gatatcaaag gcaaaacgat ggtgctgtta
     2221 ggggccggag gtgcctcaac agcgattggc gcgcaggggg caattgaagg tttaaaagaa
     2281 attaaactct ttaaccgtcg ggatgagttc ttcgataaag ccctcgcctt cgcgcagcgg
     2341 gttaacgaaa acaccgattg tgtcgtcacg gttaccgatc tcgccgatca gcaagccttt
     2401 gctgaagtcc tggcttccgc cgacatttta accaatggca caaaagtagg tatgaacccc
     2461 cttgagaatg aatcattggt taatgatatc agtctgttac atccgggact gctggtcact
     2521 gaatgcgtgt ataacccgca tatgacgaag ttattgcagc aggcacaaca agcaggttgc
     2581 aaaacgattg atggatacgg catgttgttg tggcaagggg ctgaacagtt cacgttatgg
     2641 actggcaaag atttccctct ggaatatgtt aaacaggtca tggggttcgg tgcctgacag
     2701 gctgaccgcg tgcagaaagg gtaaaaaatg aaaaccgtaa ctgtaaaaga tctcgtcatt
     2761 ggtgcgggcg cacctaaaat catcgtctcg ctgatggcga aagatatcgc ccgcgtgaaa
     2821 tccgaagctc tcgcctatcg tgaagcggac tttgatattc tggaatggcg tgtggaccac
     2881 tttgccgacc tctccaatgt ggagtctgtc atggcggcgg caaaaattct ccgtgaaacc
     2941 atgccagaaa aaccgctgct gtttaccttc cgcagtgcca aagaaggcgg cgagcaggcg
     3001 atttccaccg aggcttatat tgctctcaat cgtgcagcca tcgacagcgg cctggttgat
     3061 atgatcgatc tggagttatt taccggcgat gatcaggtta aagaaaccgt cgcctacgcc
     3121 cacgcgcatg atgtgaaagt tgtcatgtcc aaccatgact tccataaaac gccggaagcc
     3181 gaagaaatca ttgcccgtct gcgcaaaatg cagtccttcg acgccgatat tcctaagatt
     3241 gcgctgatgc cgcaaagtac cagcgatgtg ctgacgttgc ttaccgcgac cctggagatg
     3301 caggagcagt atgccgatcg tccaatcatc acgatgtcga tggcaaaaac tggcgtaatt
     3361 tctcgtctgg ctggtgaagt atttggctcg gcggcaactt ttggtgcggt aaaaaaagcc
     3421 tctgcaccag ggcaaatctc ggtaaatgat ttacgcacgg tattaactat tttacaccag
     3481 gcataagcaa taatatttcg gcgggaacac cctccccgcc gaacttaaaa aatatattta
     3541 accgtattta ataaaaatat ttcgtgagtc tctgtgcgct aattctccat ctggcgtagg
     3601 gaaaatcaca tctgaatcag gaattaacaa tgaaacctgt aaaaccacct cgtattaatg
     3661 gacgagtgcc ggtcctgtcg gcacaggaag cggtgaatta tattcccgac gaagcaacac
     3721 tttgtgtgtt aggcgctggc ggcggtattc tggaagccac cacgttaatt acagctcttg
     3781 ctgataaata taaacagact caaacgccac gtaatttatc gattattagt ccgacagggc
     3841 ttggcgatcg cgccgaccgt ggtattagtc ctctggcgca agaaggtctg gtgaaatggg
     3901 cattatgtgg tcactgggga caatcgccgc gtatttctga actcgcagaa caaa
//
LOCUS       DAJTXQ010000227         3915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000227 DAJTXQ010000000
VERSION     DAJTXQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.230"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..301
                     /locus_tag="OO515_004056"
     CDS             <1..301
                     /locus_tag="OO515_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577830.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="OO515_004057"
     CDS             390..2258
                     /locus_tag="OO515_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR6577831.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>3915
                     /locus_tag="OO515_004058"
     CDS             2295..>3915
                     /locus_tag="OO515_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6577832.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1230 a          721 c          766 g         1198 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtcgg tgatggcggc gctggccccg gtgaggaaca tatagaacga ttaacacgta
     3721 tacgcaatat tgatggctta cctcatgttg atttcagccg tgttgataag ttcttcactt
     3781 acgccgacgc ctttcgcgaa agcttgccca ttatttcagg tgaactttat ttcgaggctc
     3841 atcaagggtg ctttacatca gagtcagcaa ctaaagctca taatcgaaat atggaaaata
     3901 aattacatga tgctg
//
LOCUS       DAJTXQ010000228         3900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000228 DAJTXQ010000000
VERSION     DAJTXQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.231"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            14..580
                     /locus_tag="OO515_004059"
     CDS             14..580
                     /locus_tag="OO515_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6577833.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            584..1159
                     /locus_tag="OO515_004060"
     CDS             584..1159
                     /locus_tag="OO515_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6577834.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1201..2253)
                     /locus_tag="OO515_004061"
     CDS             complement(1201..2253)
                     /locus_tag="OO515_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6577835.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2478..3398
                     /gene="lpxL"
                     /locus_tag="OO515_004062"
     CDS             2478..3398
                     /gene="lpxL"
                     /locus_tag="OO515_004062"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6577836.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3570..3767
                     /locus_tag="OO515_004063"
     CDS             3570..3767
                     /locus_tag="OO515_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6577837.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          968 a          985 c          949 g          998 t
ORIGIN      
        1 attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta tatctgcggc
       61 cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc tgtggatggt
      121 cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata aaagcatcgg
      181 tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc
      241 gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg gacatctcgc
      301 attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa ctgccgatgg
      361 taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg acgctggcgc
      421 acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc
      481 cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg acactctgaa
      541 gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa aaagcctgct
      601 tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg ccgattacaa
      661 aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc ttggctatag
      721 ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg aaaaaaatcc
      781 ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta atcacgccga
      841 acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc cacaggcaac
      901 attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg gcgatctgac
      961 gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga
     1021 cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc tcaaggactt
     1081 caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta tttcagtgga
     1141 aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg gcaatgatat
     1201 ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag acttattaaa
     1261 gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc
     1321 gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc atactggaca
     1381 ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt cgcacggcgc
     1441 gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca tccgctcgtc
     1501 aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc
     1561 gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat gtttcatcca
     1621 ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt ttttatcttt
     1681 atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat ctgccggaat
     1741 ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca tgtcgataaa
     1801 tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat actcaccaac
     1861 attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat cgcgtacctt
     1921 catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca ggcgtaaacc
     1981 ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat tgctcgcagg
     2041 tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa aaacattcag
     2101 cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata
     2161 cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg cttttaacgc
     2221 gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg agatgggaaa
     2281 gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac attttacgct
     2341 acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc ccagtcttca
     2401 gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc ccgaataggt
     2461 tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg cttcatccgc
     2521 gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa ttgccctacc
     2581 cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt atgaaacgac
     2641 gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc gaacaagaac
     2701 gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg gaaaccggta
     2761 tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc ggcatggaac
     2821 acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc cattttctga
     2881 cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc gtttaccgcc
     2941 cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc tcaaataaat
     3001 cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg
     3061 tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc ccgttatttg
     3121 ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg tccggcgcat
     3181 gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa ttgattatgc
     3241 tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg tggatgaaca
     3301 aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta caccgtcgct
     3361 ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc ggatgcttca
     3421 gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa gctctctgga
     3481 ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc ttatgttcac
     3541 cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac acccctataa
     3601 actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt gccgccttca
     3661 gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc ggtcactccg
     3721 ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata cactacctga
     3781 gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag cagataaaag
     3841 gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag aaccctgatt
//
LOCUS       DAJTXQ010000229         3878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000229 DAJTXQ010000000
VERSION     DAJTXQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.232"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..689)
                     /gene="ychE"
                     /locus_tag="OO515_004064"
     CDS             complement(42..689)
                     /gene="ychE"
                     /locus_tag="OO515_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6577838.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1167..3842
                     /gene="adhE"
                     /locus_tag="OO515_004065"
                     /gene_synonym="adhC"
     CDS             1167..3842
                     /gene="adhE"
                     /locus_tag="OO515_004065"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6577839.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1063 a          972 c          907 g          936 t
ORIGIN      
        1 aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg
       61 aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc
      121 ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc
      181 atgcggaaca atccccagca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac
      241 agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc
      301 cccgccatca acggcaacgc caatggcacc acaccaatgc tttcacgtac cgcggtttct
      361 gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat cgacatcgct
      421 atcgtcacca ccaggatgcc ccctgcgata cggaacgaat caattgatat accaaaaagt
      481 tgcagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg
      541 gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag
      601 acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa
      661 acgggaaaat caaaaaaggt ctgaatcacg gttagctccg aagcaaaagc cggataatgt
      721 tagccataaa ttaaggttga aaagacgcgc tgacaatacg ccttttgaca gcatttttca
      781 cctcctaact acttaaaatt gctatcattc gttattgtta tctagttgtg caaaacatgc
      841 taatgtagcc accaaatcat actacaattt attaactgtt agctataatg gcgaaaagcg
      901 atgctgaaag gtgtcagctt agcaaaaatt tgatttggat cacgtaatca gtacccagaa
      961 gtgagtaatc ttgcttacgc cacctagaag tgacgcatta gagataataa ctctaatgtt
     1021 taaactcttt tagtaaatca cagtgagtgt gagcgcgagt aagcttttga ttttcatagg
     1081 ttaagcaaat catcaccgca ctgactatac tctcgtattc gagcagatga tttactaaaa
     1141 aagtttaaca ttatcaggag agcattatgg ctgttactaa tgtcgctgaa cttaacgcac
     1201 tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa gagcaagtag
     1261 acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa
     1321 tggccgttgc cgaatccggc atgggtatcg tcgaagataa agtgatcaaa aaccactttg
     1381 cttctgaata tatctacaac gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag
     1441 acgacacttt tggtaccatc actatcgctg aaccaatcgg tattatttgc ggtatcgttc
     1501 cgaccactaa cccgacttca actgctatct tcaaatcgct gatcagtctg aagacccgta
     1561 acgccattat cttctccccg cacccgcgtg caaaagatgc caccaacaaa gcggctgata
     1621 tcgttctgca ggctgctatc gctgccggtg ctccgaaaga tctgatcggc tggatcgatc
     1681 aaccttctgt tgaactgtct aacgcactga tgcaccaccc agacatcaac ctgatcctcg
     1741 cgactggtgg tccgggcatg gttaaagccg catacagctc cggtaaacca gctatcggtg
     1801 taggcgcggg caacactcca gttgttatcg atgaaactgc tgatatcaaa cgtgcagttg
     1861 catctgtact gatgtccaaa accttcgaca acggcgtaat ctgtgcttct gaacagtctg
     1921 ttgttgttgt tgactctgtt tatgacgctg tacgtgaacg ttttgcaacc cacggcggct
     1981 atctgttgca gggtaaagag ctgaaagctg ttcaggatgt tatcctgaaa aacggtgcgc
     2041 tgaacgcggc tatcgttggt cagccagcct ataaaattgc tgaactggca ggcttctctg
     2101 taccagaaaa caccaagatt ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt
     2161 tcgcacatga aaaactgtcc ccgactctgg caatgtaccg cgctaaagat ttcgaagacg
     2221 cggtagaaaa agcagagaaa ctggttgcta tgggcggtat cggtcatacc tcttgcctgt
     2281 acactgacca ggataaccaa ccggctcgcg tttcttactt cggtcagaaa atgaaaacgg
     2341 ctcgtatcct gattaacacc ccagcgtctc agggtggtat cggtgacctg tataacttca
     2401 aactcgcacc ttccctgact ctgggttgtg gttcctgggg gggtaactcc atctctgaaa
     2461 acgttggtcc gaaacacctg atcaacaaga aaaccgttgc taagcgagct gaaaacatgt
     2521 tgtggcacaa acttccgaaa tctatctact tccgccgtgg ctccctgcca atcgcgctgg
     2581 atgaagtgat tactgatggc cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca
     2641 acaatggtta tgctgatcag atcacttccg tactgaaagc agcaggcgtt gaaactgaag
     2701 tcttcttcga agtagaagcg gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg
     2761 caaactcctt caaaccagac gtgattatcg cgctgggtgg tggttccccg atggacgccg
     2821 cgaagattat gtgggttatg tacgaacatc cggaaactca cttcgaagag ctggcgctgc
     2881 gctttatgga tatccgtaaa cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa
     2941 tgatcgctgt caccaccact tctggtacag gttctgaagt cactccgttt gcggttgtaa
     3001 ctgacgacgc tactggtcag aaatatccgc tggcagacta tgcgctgact ccggatatgg
     3061 cgattgtcga cgccaacctg gttatggaca tgccgaagtc cctgtgtgct ttcggtggtc
     3121 tggacgcagt aactcacgcc atggaagctt atgtttctgt actggcatct gagttctctg
     3181 atggtcaggc tctgcaggca ctgaaactgc tgaaagaata tctgccagcg tcctaccacg
     3241 aagggtctaa aaatccggta gcgcgtgaac gtgttcacag tgcagcaact atcgcgggta
     3301 tcgcatttgc gaacgccttc ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc
     3361 agttccatat tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca
     3421 atgcgaacga caacccgacc aagcagactg cattcagcca gtatgaccgt ccgcaggctc
     3481 gccgtcgtta tgctgaaatt gccgaccact tgggtctgag cgcaccgggc gaccgtactg
     3541 ctgctaagat cgagaaactg ctggcatggc tggaaacgct gaaagctgaa ctgggtattc
     3601 cgaaatctat ccgtgaagct ggcgttcagg aagcagactt cctggcgaac gtggataaac
     3661 tgtctgaaga tgcattcgat gaccagtgca ccggcgctaa cccgcgttac ccgctgatct
     3721 ccgagctgaa acagatcctg ctggatacct actacggtcg tgattatgta gaaggtgaaa
     3781 ctgcagcgaa aaaagaagcc gctccggcta aagctgagaa aaaagcgaaa aaatccgctt
     3841 aatcagtagc gctgtctggc aatataaacg gccccttc
//
LOCUS       DAJTXQ010000230         3833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000230 DAJTXQ010000000
VERSION     DAJTXQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.233"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..266)
                     /locus_tag="OO515_004066"
     CDS             complement(<1..266)
                     /locus_tag="OO515_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6577840.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="OO515_004067"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="OO515_004067"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6577841.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="OO515_004068"
     CDS             complement(726..998)
                     /locus_tag="OO515_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6577842.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="OO515_004069"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="OO515_004069"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6577843.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="OO515_004070"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="OO515_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          998 a          932 c          917 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tga
//
LOCUS       DAJTXQ010000231         3820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000231 DAJTXQ010000000
VERSION     DAJTXQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.234"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1139)
                     /gene="garK"
                     /locus_tag="OO515_004071"
     CDS             complement(<1..1139)
                     /gene="garK"
                     /locus_tag="OO515_004071"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6577844.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNA"
     gene            complement(1236..2126)
                     /gene="garR"
                     /locus_tag="OO515_004072"
     CDS             complement(1236..2126)
                     /gene="garR"
                     /locus_tag="OO515_004072"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6577845.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2156..2926)
                     /gene="garL"
                     /locus_tag="OO515_004073"
     CDS             complement(2156..2926)
                     /gene="garL"
                     /locus_tag="OO515_004073"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6577846.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(2942..>3820)
                     /gene="garP"
                     /locus_tag="OO515_004074"
     CDS             complement(2942..>3820)
                     /gene="garP"
                     /locus_tag="OO515_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6577847.1"
                     /translation="FNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWI
                     KLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLG
                     VFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDY
                     LIKRGLSLTLARKLPIVLGMLLVSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPV
                     ISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMV
                     CYLFVVGDIKRMELQK"
BASE COUNT          922 a         1115 c          896 g          887 t
ORIGIN      
        1 gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac gtgaagcacg gcagatattg
       61 tcataagccc cgcggaatgc ttcgtccaac gtacctatgc tggtcaatac gctgaagacc
      121 gcatcgatgc catgctgatg tacaacgcca acatcatcgg tcaggctacc cgcaatgcca
      181 atcaccggtt tatggtactt cttcgccacg tttgcaacgc caatcggtac cttgccgtga
      241 atactctggc tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc gtgaatatgt
      301 tcttccagat tcagcgccgt agtgacgatt tcaataccac ttttcagttc cgcaccaaga
      361 aacgccatca gcgccgcgcc cataccgccc gcagctcctg cgccggggac atctttcaca
      421 tcaacatgca gcgctttttt aatgacatcg gcatagtgag agaggttatt gtccagctca
      481 acaatcatcg cttcactggc tcccttttgt gggccaaaga tgcgcgatgc gccgttatcg
      541 cccaccagcg gattggtgac atcacaagcg acgcgaatga cgcaatcttt taagcgcgga
      601 tcgaggccgg aaatatcaat atcattcaga gtattaagac taccgccgcc aaaaccaatt
      661 tcattgccgt tggcgtcgca taatttcgcc cccagcgcct gtaccatgcc tgcgccgcca
      721 tcatttgtag cgctgccgcc aatgccgata ataatgtttg ttgcactgct ctccagcgcc
      781 tgcaggatta actcgcctgt gccgcgtgaa gtggttacga gtggatcgcg tttttccgca
      841 ggtaccagct ccagcccact ggccgccgcc atttcaataa acgcggtttt gccatcgccg
      901 gagatccccc aactggcatt cactttctcg cccagcggcc ctgtaaccca ggcgtgacgt
      961 tcagccccct gggtggctgc aatcatcgct tccaccgttc cttcaccacc gtcggcaacc
     1021 ggaacagaaa catactgtgc atcaggaaaa atttcccgaa atcctttttc tatcgcctgc
     1081 gcaacctcgc tggcagataa actttcttta taagagtctg gggcgattac gattttcata
     1141 cctatgcctg ttaccacatg acgccggagg gcgtttctct tattcggcct agattccagg
     1201 cccggattgc aatacaccat ccgggcacga cgtcattaac gagtaacttc gactttcgcc
     1261 agtttttcgt agtagcacgc cagggcgcta tgatccgccg ttcctaaacc atctgcacgc
     1321 agtgcctgca tcatctccat aaccgcagct gtgagcagca gttgtgcgcc gacgccgtga
     1381 gaagtatcca gcgcattcgc cagatcctta atatgcagat caatgcggaa gcccggcttg
     1441 aagttgcggt ccatcaccat cggcgctttg gcatccagca cggtactgcc cgccagccca
     1501 ccgcgaattg cctgataaac caggtccggg ttaacgcccg ctttggttgc cagcgttaac
     1561 gcttctgaca tcgcagcaat attcagcgcc acaatgacct gatttgccag tttggtgacg
     1621 ttacctgcac cgatttcccc ggtatgcacc acggaacccg ccatcgcttt catcaaatca
     1681 tagtatttgt cgaaaatagc cttgtcgccg cccaccatca ctgacagcgt accgtcgatg
     1741 gctttcggtt caccgccgct caccggagca tccagcatat caatgccttt cgctttcagc
     1801 gcttcgctga tttcacggct tgccagcggt gcgatagaac tcatatcgat caataccgta
     1861 cctggcttcg cgccttcaat aatgccattc tcacccagcg ccacctcttt cacatgaggg
     1921 gagtttggca gcatggttat gatgacgtcg cactgttcag cgatcgcttt agccgtagac
     1981 gctgtttctg cacctgcagc aatcacgtca gcaatagctt ctgggttacg gtcagcaacc
     2041 accagcgagt aacctgcttt cagaaggttt ttactcattg gtttacccat aatccccagg
     2101 ccaataaaac caactttcat agtcatatca accatctctc ttgttgcggt ggtggttatt
     2161 ttttaaaggt atcagccagt ttctgagtgg cagagcggaa gacgccaaga tcgctgccga
     2221 cagccacaaa cgtcgcgccc cattccagat aacgacgcgc atcggcttcg accggcgcga
     2281 gaataccgct gggtttgccg tgcgcgctgg cacggttaaa aatatgctga attgcttttt
     2341 gtacatccgg atgcgaggca ttgccgagat ggcccaatgc cgcagccaga tcgctggggc
     2401 cgacgaagat accgtctaca ccttcggtag cggcaatagc atcgacgtta tctacgccct
     2461 gctgactttc tatctggacc agaatagtaa tgttcttgtt cgactgagcg aaataatccg
     2521 ccacggtgcc aaacatattg gtgcggtgag aaacggagac gccgcgaatg ccttccggcg
     2581 ggtaacgagt tgatgccacc gcctgctctg cttcctcttt tgtttctaca aaaggaatca
     2641 ggaagttata gaaaccgata tccagaagac gcttaataat taccggctcg ttggtcggca
     2701 ctcgcactac tggcgcgctg gcgctgcctt tcagggccat taactgcgga ataaacgtgg
     2761 aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca gtcaaaccca gccaaaccaa
     2821 gaacttcagt gctaatcggg ttagagagtg ctgaccagca accaatttgt acctgtttcg
     2881 cagccagtgc ggctttgaat ttattcggga aaacatcgtt attcatcgct tatacctttg
     2941 cttatttctg caattccata cgtttaatgt cgccaactac gaagaggtag cagaccatcg
     3001 ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt gaaggagtgc agttcactta
     3061 ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac attgccaaag acgttaaaga
     3121 cgccgccaca gaggccaaca atctctttcg gcgcggtgtc agaaatcacc ggccagccca
     3181 gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag catgaccacc agcgtggtgt
     3241 tgttggtgta gttacataag atgatggtgg aaaccagcaa cattcccagc acaatcggta
     3301 gcttacgtgc cagggtcagg gataaaccgc gtttgatcag ataatccgag aagacacctc
     3361 ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga ggcgaccaga cccactttca
     3421 gaatcgacat gcctttttcc tgcaccagat aaatcgggaa ccaggtgagg aagaaccagg
     3481 tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa catcatgcgg ttagagagca
     3541 attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc gctgcccggc tttttgtggt
     3601 ccatatcgac caccgcgcca ttttcagaga taaacttcag ctcttccgca gacatacgtg
     3661 ggtgatctgt cgggttatga atcaacttga tccacagcgc cgtcagcaca aaaccaatcc
     3721 cccccataac ggtgaagacg tgctcccagc cccaagcgaa agtcagccag ccaagcagcg
     3781 gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa
//
LOCUS       DAJTXQ010000232         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000232 DAJTXQ010000000
VERSION     DAJTXQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.236"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            702..1418
                     /gene="nanC"
                     /locus_tag="OO515_004075"
     CDS             702..1418
                     /gene="nanC"
                     /locus_tag="OO515_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6577848.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1438..2544
                     /gene="nanM"
                     /locus_tag="OO515_004076"
     CDS             1438..2544
                     /gene="nanM"
                     /locus_tag="OO515_004076"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6577849.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2609..3589
                     /locus_tag="OO515_004077"
     CDS             2609..3589
                     /locus_tag="OO515_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6577850.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1141 a          787 c          867 g         1011 t
ORIGIN      
        1 ggtgagaatt atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag
       61 ttagcttgca aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt
      121 aaaagaaatc ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat
      181 cttcctctta gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg
      241 tatggcttgc gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa
      301 tgagacaggg catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga
      361 tgaaaacgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta
      421 tgactctggg taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa
      481 acatcagaag agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg
      541 tggaaagaga attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc
      601 aaagacacta taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg
      661 gctttgtatt gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac
      721 tttctggcgt attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg
      781 acgtacgtgg tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg
      841 agggatggca aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg
      901 ataataaaaa ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta
      961 aacttgatga tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca
     1021 cacgctacgg accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca
     1081 ttcgctatcg ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc
     1141 gtgataacgt tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct
     1201 tcgcatggca aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga
     1261 aatgggcgac ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat
     1321 acatagaata tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg
     1381 aaaacagtta tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg
     1441 aataaaacta taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta
     1501 ttaccggaaa ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc
     1561 tacattggtt taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat
     1621 aaaaaatgga ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca
     1681 tttattgatg gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact
     1741 caggtattta atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg
     1801 tcgctcgcgc cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat
     1861 gttactggcg gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct
     1921 ggaaaagatt caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa
     1981 gattatttct tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac
     2041 gctggcgaat cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa
     2101 acctggctta ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt
     2161 gatttcaccg gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc
     2221 gtcgctggcg gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga
     2281 ttcaaaggtt cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa
     2341 aaatcatata gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa
     2401 ttatcgcaag gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt
     2461 ggcggtgaaa ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat
     2521 aataaagtca cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa
     2581 tttgtcattt gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta
     2641 tgttcttacc gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc
     2701 ggacagggaa gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca
     2761 tcccggaggc ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga
     2821 tgttcaggat atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg
     2881 caccgttggc caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc
     2941 agggattctt atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg
     3001 gacatattca gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc
     3061 gctgtaccag gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa
     3121 attcctcggc gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc
     3181 acaccctcaa cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca
     3241 ttctcagctt aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg
     3301 gaaagaaaat ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt
     3361 agccaatatt attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc
     3421 tgatgaagac ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga
     3481 gaactggacg acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat
     3541 ttctgacaga tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt
     3601 attcatgggg gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa
     3661 ggtaatatct cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc
     3721 acaagggtga aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg
     3781 ccttaataga catccgcgcc ttttcg
//
LOCUS       DAJTXQ010000233         3798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000233 DAJTXQ010000000
VERSION     DAJTXQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.237"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="OO515_004078"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="OO515_004078"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6577851.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="OO515_004079"
     CDS             1027..1485
                     /locus_tag="OO515_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6577852.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="OO515_004080"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="OO515_004080"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6577853.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="OO515_004081"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="OO515_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6577854.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="OO515_004082"
     CDS             2691..3368
                     /locus_tag="OO515_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6577855.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="OO515_004083"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="OO515_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6577856.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          953 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcctt
//
LOCUS       DAJTXQ010000234         3770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000234 DAJTXQ010000000
VERSION     DAJTXQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.238"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..354)
                     /locus_tag="OO515_004084"
     CDS             complement(<1..354)
                     /locus_tag="OO515_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6577857.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGS"
     gene            complement(351..1748)
                     /locus_tag="OO515_004085"
     CDS             complement(351..1748)
                     /locus_tag="OO515_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6577858.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            1903..2901
                     /locus_tag="OO515_004086"
     CDS             1903..2901
                     /locus_tag="OO515_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6577859.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3012..>3770)
                     /gene="ompG"
                     /locus_tag="OO515_004087"
     CDS             complement(3012..>3770)
                     /gene="ompG"
                     /locus_tag="OO515_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6577860.1"
                     /translation="LAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELE
                     VHYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNG
                     WLSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEF
                     STQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQN
                     GLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT          932 a         1013 c          921 g          904 t
ORIGIN      
        1 agaacctacg ccagcgccga taatcaatgc cccagcggca catccaccca gcgctaccca
       61 gtcctgggtt tgccaggcat tcattgtcca ctgtacaccc tgcccgacaa cacttgcgcc
      121 aaacagagcc agcccgccca tcaccatttt gcgccacagg ctgcgtttcg gacgtaacgc
      181 tgcatccatt accgcttcaa cttgcccctc ttcttcaggc gcttcatcga gcgtggccgg
      241 ggcaaaattt tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt ttggattctg
      301 atcgacctcc agcggaccgt cgaaatcaat acgtggtttt aacggttcgg tcatcgcaat
      361 ttatctccta ttaaaaattc cagcgcggca tcaagacgaa tatgcggcag cggtttgtcg
      421 acatccatca cctgcgggcg aaacgcttca aactggaacc cttgcttgtc ccagaacgcc
      481 tgaccgggca aacgcgcagg aacttcaccg gggtaaacgg tgagaggcgc accatcgctg
      541 agccgattac cgcgcaatgc cggaattttc tcgccgttga catcaataat gccgctggtg
      601 gtcgcctgca ccgaggccag ccccaggcaa tccatactaa tcccttcaaa cgccgcattt
      661 tgccaggcat cctgaatcag ttgttgcagt aatgaaacca tattggcgtg ctgatcgacc
      721 gttacatggt ccgctttcgt tgcggcaaac agcagtttgt cgataaccgg cgagaataag
      781 cggcgaaaga gcgtgcgctg cccgtagtgg aaactttgca tcagctgcgt cagtgccaga
      841 cgcatatcat taaatgcctg tggcccactg ttgagaggtt gcaggcaatc caccagcaca
      901 atctggcggt caaagcgcag aaaatgattc ttatagaacc ccttcaccac cttctcgcag
      961 taataattaa accgctcgcg cagcattccg gcattggtgt gcttatcggc ctgcgccagt
     1021 ttggactcgc cccaggtatc gacatccggc cacgggaaga attgcagcgc gggcgcacct
     1081 gccatatctc ctggcaagac aaagcgccct ggctgaataa agtgcagccc ctgctgttta
     1141 cagtggtgga gataatcggt ccacgcggcg gcaatgtctg ccagccggtt ttcgtcggca
     1201 ggtgctagcg ggtccagccc ttcgcacatc attcgccatt tgaccgacca ttcgccgcgc
     1261 tgaccattga gtaagcccgt catctggcgt gaccagctta aatagtcctg cgccagcatc
     1321 ggcaggtcga gcaaccattc gccgggataa tccacaatct ccagatacag cgtggaggtg
     1381 tctttgaagt ggcgtagcag cgaatcgttc gatttaaaac gtagcgccag gcggatttca
     1441 ctgacgccgc gcgtaggcgt cggccaggcg ggaggatcgc catataactg cgccagtcct
     1501 tcatcatagg tgaagcgcgg aatgccaaag tcacgctgag gaatgcgttt tacgcccagc
     1561 aggcgctctt cacgcaccgc gcttaacagc ggcaagcgtg ctccggcgtg aatattgagc
     1621 aactgattga ccatcgcagt gataaacgct gttttgccgc tgcggcttag tccggttacg
     1681 gcgaggcgca gatgtctgtc gacaccccga ttcaccagcg cattaagttc atttttaagt
     1741 cgcttcattg ctgtcctgtg ctgctctgaa gaccagaaaa aaatcattcg taaggatatg
     1801 aatgtggggg ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg ggcgaactcc
     1861 ataagatgtg cagcattcac gttcggacgg agttcctgac ctatgtcccc tactatttat
     1921 gatattgcca gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct gaataagcaa
     1981 actaatatct ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga attacagtat
     2041 cagccaaaca agctagcccg cgcgctgacc tcttcgggtt ttgatgccat tatggtgatt
     2101 tctacccgtt cgaccaaaac tacggcgggt aatccgtttt tctctgaagt tttgcatgcc
     2161 atcaccgcca aagctgaaga agaaggtttc gacgtgatat tgcagacgtc gcacaacccg
     2221 gcagaagact tacaaaaatg tgaaagcaaa attaagcaga aaatgattaa aggcattatt
     2281 atgctaagtt cgccagcgga tgagtcattt tttgcccaac tcgataaata cgatattccg
     2341 gtggtggtga ttggcaaagt tgaaggtcaa tatgcccatg tttattctgt cgataccgat
     2401 aattttggcg acagcattgc gttgaccgat gcattaattg aaagtgggca tcaaaatatt
     2461 gcctgcctgc atgctccgct tgatgtccat gtttcagtgg atcgggtaaa tggttataag
     2521 cagagcctgg ctgcgcataa tattgcagtg cgtgatgaat tgattgttga cggtggttat
     2581 acccatgaaa cagcactgca agccgcgaga gaattattaa gccagtcacc gttgcctgag
     2641 gcggtgtttg ccactgacag tctgaaatta atgagtattt atcgtgcggc agcagagaaa
     2701 aatattgcta ttccgcagca gttagcggtg gtgggttata gtaatgaaac gctgtcgttt
     2761 atattaacgc ctgcaccggg cggcatcgat gttccgacgc aggagttagg tcaacaaagc
     2821 tgcgagttat tattccgctt aatttctgga aaaccgtcac cacagaatat taccgttgcc
     2881 acgcatatga cgctgaaata aataccagct accttgcctg aatgccggat gcgatgctag
     2941 cgcatcttat ccggcctaca tttatagatc ggataaagta ctcgcgccgc atccggcaat
     3001 ttagcccatt atcagaacga gtaatttacg ccgacacctg cataatggaa tttatcactg
     3061 tctccttcgt cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga aagtccgttc
     3121 tggaaatcat aaccgtaaaa taaacctaca cggttaaaat catggccttc acgttcaata
     3181 tcatcctgcc agtcccagtt actccagcga tccagcccaa tgcgcgtata cggcgtcacc
     3241 gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt cttgcgtgga aaactcaccg
     3301 ttattgcggc tgtcgtccat attgaagccg cgctcgagat aatagttcac tcgcaaggca
     3361 accgtttcgt tgaaggtata ttgcagacct gtttccgttt cgacacgggt atcagcgtaa
     3421 ccggtagtgt tcagatcgtt ggcaaattta tacatcgaca accaaccgtt gaaacgtaaa
     3481 tcgtcagtca gtttcacatc ccagtctggc gcgattttcc agcgctgcat attcgccgtg
     3541 tctttacccg gttcatcaac gtagtgataa ccataattac ggaaaccgcc ggtcaggccg
     3601 aaactgaaat catcgttttc gaggaactga taatgcacct ccagctccgg gcgatcaaac
     3661 cacgttccac gtttacccgc gctataatct accggccctt cctgataata ggccagagca
     3721 attctccacg gcccgttggc ggcattaaaa tagactgaag gctccgccag
//
LOCUS       DAJTXQ010000235         3759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000235 DAJTXQ010000000
VERSION     DAJTXQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.239"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..559
                     /gene="adeQ"
                     /locus_tag="OO515_004088"
                     /gene_synonym="yicO"
     CDS             <1..559
                     /gene="adeQ"
                     /locus_tag="OO515_004088"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6577861.1"
                     /translation="SFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSS
                     VAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAG
                     ALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCT
                     GRWRDLNLCVVVVASLFALKIILVD"
     gene            612..1064
                     /locus_tag="OO515_004089"
     CDS             612..1064
                     /locus_tag="OO515_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6577862.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1275..2455
                     /gene="nepI"
                     /locus_tag="OO515_004090"
                     /pseudo
     CDS             1275..2455
                     /gene="nepI"
                     /locus_tag="OO515_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2496..2789)
                     /locus_tag="OO515_004091"
     CDS             complement(2496..2789)
                     /locus_tag="OO515_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577863.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3011..>3759
                     /gene="nlpA"
                     /locus_tag="OO515_004092"
     CDS             3011..>3759
                     /gene="nlpA"
                     /locus_tag="OO515_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6577864.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQS"
BASE COUNT          833 a          850 c          996 g         1080 t
ORIGIN      
        1 ctcctttatg ctgatcaacc tatttgattc atcaggaaca ttaattggtg taactgataa
       61 agcgggctta atagatggta acggtaaatt ccccaatatg aataaggcgc tgtatgttga
      121 tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg tctgttactg cctatattga
      181 aagtacttct ggtgtggcag tcggtggccg cacggggctg actgcggttg tggttggcgt
      241 tatgttcctg ttggttatgt tcttctcccc gctggtggcg atggttcctc cttacgcaac
      301 cgccggagcg ttaatctttg ttggcgtgct gatgacttcg agcctggcgc gcgttaactg
      361 ggatgatttt accgaatcgg tgcctgcgtt tattaccacg gtgatgatgc cctttacttt
      421 ctcgatcacc gaagggattg cactcggctt tatgtcgtac tgcatcatga aagtgtgcac
      481 cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc gcatctctgt ttgcactgaa
      541 gattattctg gtggattagt aactggcgag ggtttccaga tatcatgagt tctgataacg
      601 caggagaact catgatctgg ataatgctcg ccacgctggc ggtagtgttt gtggttggtt
      661 ttcgggtgct gacatccggg gccagaaaag cgattcgccg tctcagtgat cggctgaata
      721 tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg aaagtcagcc ggtgacgaat
      781 ttttacgtta tttgcatcgc ccggatgagt cgcacctgca aaacgccgcg caggtgttgc
      841 tcatctggca aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga
      901 ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc tcaggtcagg ctggcgctgg
      961 gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag atgcgctata
     1021 acgcgttctt tcagcctgcc gagggcgttc actggctgca ttaaaaccgc ttcgtcttat
     1081 cagcctacag accgcgcctg acctgtcggg tggataagac gttcacgccg catccggtaa
     1141 tctttgccct gttccgtttc gatcttaact ccagctcaag caacaccgta aaagttgcaa
     1201 aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc ggcttttccc ctgaaacatg
     1261 ccacgggtaa caccatgagt gaatttattg ccgaaaaccg cggcgcggat gccatcaccc
     1321 gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt cgcctgtctg attatcgttg
     1381 agtttttgcc cgtcagtttg ttgacgccaa tggcccagga tttaggcatt tcggaagggg
     1441 ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat gtttgccagt ttgtttatta
     1501 cccagacaat tcaggctact gaccgccgca acgttgttat tttgtttgcc gttttgctga
     1561 cgctctcctg cttgctggtt tcctttgcta actcattcag tttgctttta atcggtcgtg
     1621 cctgtctggg gctggcgctg ggcgggttct gggcgatgtc ggcgtcgctg accatgcgtc
     1681 tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat cttcggcgcg gtttctattg
     1741 cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga gcttatcggt tggcgcaatg
     1801 tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt ctggattatc aaatcattgc
     1861 cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac tttccgctta ttacaacgtc
     1921 cgggtgtgat ggcagggatg atcgccatct tcatgtcttt cgccgggcag tttgctttct
     1981 tcacatatat tcgcccggtg tatatgaacc tggcgggatt cagcgtggat ggcttaacgc
     2041 tggtgctgtt gagttttggc attgccagct ttatcggtac gtcgctttcg tcattcattc
     2101 ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt aatactggct gtgagtgcgt
     2161 tggtgctgac gttgtgggga agcgataaaa tcgttgctac cggcgtggcg attatctggg
     2221 ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg gatcacccgc tcactggccg
     2281 atcaggcaga aaaagccggg tctattcagg tggcggttat tcagcttgtg gcgcggcaat
     2341 cggcggttat gcgctggata atattggtct gacttcgccg ttgatgtttt ctggcacatt
     2401 gatgttgctg actgcattgt tggttactgc aaaggtgaaa atgaagaaat cctgatatca
     2461 ggtaaagaag cgttggtcag tagttgttgc tgttcttaca tatccgggca ttctacctta
     2521 cccaacgctt cccaataatt gctctgatta ttacgttcca tcgccacgat ggcctcgcgt
     2581 atatgttgtt tattgttttc atctgacaac attaactttt cccacgcttc atctgataat
     2641 gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata ccgtggtttt ctcttttgcg
     2701 gactgcagtg agctaaaagg cgactcggcg tatacgatgc ctggcatagc gaaaaaagtg
     2761 aaaataagca gtagtgtcgt tggtttcatg gattcaacct tttacaaatg tgtatcgaat
     2821 gaagcataag tgtagtttgc tttcatcgct ctcatcatgc tgtacgaaac gcgcttgcgt
     2881 ggtcagtaag aagtgccaga ctttatattc cacttttatt cctttttatt cttatcgata
     2941 gcgtttcgtt ttttaaaccg cagcgacctt accgctatag tcaggtaatc attaataaaa
     3001 ggataaaaaa atgaaactga caacacatca tctacggaca ggggccgcat tattgctggc
     3061 cggaattctg ctggcaggtt gcgaccagag tagcagcgat gcaaaacaca ttaaagttgg
     3121 cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct aaaaaggtgg caaaagagaa
     3181 atatggcctc gatgttgaac tggtaggatt tagcggttcg ttgctaccga acgatgcgac
     3241 aaatcatggc gaacttgatg cgaatgtctt ccagcatcga ccctttcttg aacaagataa
     3301 tcaggcgcat ggttataaac tggtagcgct gggaaatacg tttgtcttcc ctatggcggg
     3361 ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa ggcgcgacag tggcgatccc
     3421 caacgacccg accaaccttg gtcgcgcact tttactgttg caaaaagaga agctaattac
     3481 tctgaaagag ggaaaagggt tattacctac cgcgctggat ataaccgata atcctcgtca
     3541 tttgcagatt atggaactcg agggggcgca gctcccgcgc gtactggatg atcccaaagt
     3601 tgatgtagcg attatcagca ccacttacat tcagcagaca gggctttctc cggtgcacga
     3661 cagcgtattt attgaagata agaattcgcc gtatgtgaat attttggtgg cacgggaaga
     3721 taataagaat gcagaaaacg tgaaggaatt tctgcaatc
//
LOCUS       DAJTXQ010000236         3734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000236 DAJTXQ010000000
VERSION     DAJTXQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.240"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..181)
                     /locus_tag="OO515_004093"
     CDS             complement(<1..181)
                     /locus_tag="OO515_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCR6577865.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="OO515_004094"
     CDS             425..682
                     /locus_tag="OO515_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6577866.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO515_004095"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO515_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6577867.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO515_004096"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO515_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6577868.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO515_004097"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO515_004097"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6577869.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="OO515_004098"
     CDS             complement(3307..>3734)
                     /locus_tag="OO515_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6577870.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAJTXQ010000237         3641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000237 DAJTXQ010000000
VERSION     DAJTXQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.241"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..753
                     /gene="dcuC"
                     /locus_tag="OO515_004099"
     CDS             <1..753
                     /gene="dcuC"
                     /locus_tag="OO515_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6577871.1"
                     /translation="AIAHFFWQRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPII
                     GVLIFDGKWGPQLHIITILVICMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANV
                     VMLLVAAGVFAQGLSTIGFIQSLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAP
                     FYAFVEMIPKLAHSSGINPAYLTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVV
                     KRTSVPVLVGLVIVIVATELMVPGTAAAVTGK"
     gene            complement(794..1474)
                     /gene="dpiA"
                     /locus_tag="OO515_004100"
                     /gene_synonym="citB"
     CDS             complement(794..1474)
                     /gene="dpiA"
                     /locus_tag="OO515_004100"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6577872.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(1443..3101)
                     /gene="dpiB"
                     /locus_tag="OO515_004101"
     CDS             complement(1443..3101)
                     /gene="dpiB"
                     /locus_tag="OO515_004101"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR6577873.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            3481..>3641
                     /locus_tag="OO515_004102"
     CDS             3481..>3641
                     /locus_tag="OO515_004102"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6577874.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGG"
BASE COUNT          933 a          931 c          816 g          961 t
ORIGIN      
        1 gcgatcgccc acttcttctg gcaacgttat ctggataaaa aagagcacat ctctcatgaa
       61 atgttagatg tcagtgaaat caccaccact gctcctgcgt tttatgccat tttgccgttc
      121 acgccgatca tcggtgtact gatttttgac ggtaaatggg gtccgcaatt acacatcatc
      181 actattctgg tgatttgtat gctgattgcc tccattctgg agttcctccg cagctttaat
      241 acccagaaag ttttctctgg tctggaagtg gcttatcgcg ggatggcaga tgcgtttgct
      301 aacgtggtga tgctgctggt tgccgctggg gtattcgctc aggggcttag caccatcggc
      361 tttattcaaa gtctgatttc tatcgctacc tcgtttggtt cggcgagtat catcctgatg
      421 ctggtattgg tgattctgac aatgctggcg gcagtcacga ccggttcagg caatgcgccg
      481 ttttatgcgt ttgttgagat gatcccgaaa ctggcgcact cttccggcat taacccggcg
      541 tatttgacta tcccgatgct gcaggcgtca aacctgggtc gtaccctatc acccgtttct
      601 ggcgtagtcg ttgcggttgc cgggatggcg aaaatctcac catttgaagt cgtaaaacgc
      661 acctcggtgc cggtgcttgt tggtttggtg attgttatcg ttgctacaga gctgatggtg
      721 ccaggaacgg cagcagcggt cacaggcaag taaatagtaa tgccggaggc aagtttcctc
      781 cggctttatc atgtcaccca ctgtggtata tgcgttgtgg tctgccaact ttgccgtgaa
      841 caatttcagc aataatcaga tggcggctgg cgcaatattc aagataacgc ctggcagtgg
      901 tgcagctgat ggtcagtgcc tgcgccaccg tttctgccgt atgttgcaca ccaggctctt
      961 taaacagttt tcgcaccgcg tttagcgtca aggggtcaat gccggtcggt agctcgtcct
     1021 taggttcacc gcgagcataa gcattaaaca tctcatcaat ttgcttctgg ctggcgctat
     1081 caatactttc cagcatatgt ttacgctggc ggaaacgggt tagcgtttgc cccagccgtt
     1141 cataggcaat gggcttaatg agataatcaa atacaccaca acgtacggct tcagacaccg
     1201 tttccatatc gctggctgca gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca
     1261 gttcatgcag taaattaatc cctctaccgt caggaagata gttatcgagc aagattagcc
     1321 ccggcttaaa acgctcgatc atcattcggg cctgcgccag atttcccgcc agtaatatct
     1381 gactgaatcc gggaatgtga cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt
     1441 cctcaacgat caatagggtt aatggagctg tcattaggtt tcactttcgg aatatagatt
     1501 gaaaataagg taccgcaggg atcattatct tcgagagtga taacaccacc gcagcgcgtt
     1561 acgtagctgg caatcaagta caacccaatg ccatgttcac cgggctcgtc agcacgcgta
     1621 ctgaccccct gctcaaatat tttgtctctt agagactctg gaacgccgca gccctgatcg
     1681 gcgacttcaa tcaccacatc atcgccttca tcgctgagga ataattcaac gatcttgttt
     1741 ccttcatcgc tacgcaggct ggcttcgaag gcgttatcaa gtaaattgcc aacaatggcg
     1801 gcaaactcgg tgctatccag tcctggcggc agttgcgaaa gctggctacc ggggacgatg
     1861 atcattttta gccccagttc gcgggcgcgc tgcactttac caaaaagcag ccccgccacc
     1921 tggcgatcgg caaacgcctc acgcaggctg tcaataagct gttgctgggc ctgagactcc
     1981 ccctgcacca tcgccagcac gcgatcatac tctttcatct gcaacagacc attgagcgtc
     2041 gacatccaat tgtgatgctc gtgtcgcaat gtacgaaggc tctcgacgta ttgttttatt
     2101 tgcgtcagtt gcgcattgag ggtggaaatt tcgtctttac tacgaaagct gataatggcc
     2161 cccagcaaat catcacctga acgaatagct tcccggttgg caataacgct cagaccatta
     2221 aagttcgcca ccacatcctg acgtttttca tcaatctgtt cggtaaagaa atcggcgggc
     2281 ctgaccactt cagcaatggg tttacccaac cattgccgtc cgggagagct cagccccagc
     2341 atctttcttg cgttacgatt gatggcggta atgtaaccat gcggatccac cgcaatcagc
     2401 ccttcataaa ccgaactaaa cagcgcctct tgctgacgga ccacgcgtgc gatttgcttt
     2461 ggctccatgc ccatcatttg tcgacggata tgcgcggcca ggaaccacga cagcaacatc
     2521 agaatcccta acagcacgac aaacacgcct gccatcggta ataaaaactc agcccgccag
     2581 ctatcgattt tactcaccag gtagccaatc gacaccacgc cgatgacttt tccatcgtca
     2641 tcaaagattg gcgttttggc gcgcatcgcc atccccattg cccctttccc ggtaatgaag
     2701 tagctctccc ctttctccag cgcgccctgt ttggtgaact gcataggata accaattttc
     2761 tccggattaa gatggtaaag gcggatcgag tgccggtcgc caatcaccac ataatcaaaa
     2821 tcggtatctc tttgtaattt gttagcgatg gtcgccagcc gtttgtagtc acgcgttttc
     2881 accgcagaga tgacactgtc attggaggca ataattttcg cctgattcat tgccatgtcg
     2941 cgtacatgaa gcgttaaata gtcctcaaaa ctggccgtaa aatattgcgc cagggctgca
     3001 atgacaaata ttgagaacac cagaatcagc aaaaagatac gcagcggaaa tgccagtctt
     3061 tggaaaaatg caaactgttt attctcgtta agctgcaaca ttgttttcct tgcgggttgt
     3121 cccgagcctc tttgacggat gtaattaaaa tgttgaatca aaatacatag atattaatta
     3181 tgatcgtggc aaattttatt cattaaatca attaaatcac ttaaacctgc aggtcatttc
     3241 gtaattacat taattaaaaa acttaaaacc attaaataaa taaaaaacca ctaactctat
     3301 gtgaaataaa tcaaaatttc acgctgcaat actccttaga atgtatagcg aaaggagaaa
     3361 aagatatacc tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcaacttgca
     3421 acattgaaca acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt
     3481 atgttcggca atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa
     3541 atcgcccaat tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt
     3601 accgtaaccc gcgatgaaaa gcttatcgcg tgcggtggaa t
//
LOCUS       DAJTXQ010000238         3562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000238 DAJTXQ010000000
VERSION     DAJTXQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.242"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            130..1377
                     /locus_tag="OO515_004103"
     CDS             130..1377
                     /locus_tag="OO515_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCR6577875.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1906..2106)
                     /gene="torI"
                     /locus_tag="OO515_004104"
     CDS             complement(1906..2106)
                     /gene="torI"
                     /locus_tag="OO515_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCR6577876.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2229..2573)
                     /locus_tag="OO515_004105"
     CDS             complement(2229..2573)
                     /locus_tag="OO515_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577877.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2815..3417
                     /locus_tag="OO515_004106"
     CDS             2815..3417
                     /locus_tag="OO515_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577878.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          715 c          817 g         1144 t
ORIGIN      
        1 tgcagtaacg gcgaaagggg caatgacagc gctgccatgt cgacaagaac tggaatagtg
       61 agaagtaaac ggcgaagtcg ctcttatctc taaataggac gtgaattttt taacgacagg
      121 taggtaatta tggcactgaa tattccattc agaaatgcgt actatcgttt tgcatccagt
      181 tactcatttc tcttttttat ttcctggtcg ctgtggtggt cgttatacgc tatttggctg
      241 aaaggacatc tagggttgac agggacggaa ttaggtacac tttattcggt caaccagttt
      301 accagcattc tatttatgat gttctacggc atcgttcagg ataaactcgg tctgaagaaa
      361 ccgctcatct ggtgtatgag tttcatcctg gtcttgaccg gaccgtttat gatttacgtt
      421 tatgaaccgt tactgcaaag caatttttct gtaggtctaa ttctgggagc gctctttttt
      481 ggcctggggt atctggcggg atgcggtttg cttgacagct tcaccgaaaa aatggcgcga
      541 aattttcatt tcgaatatgg aacagcgcgc gcctggggat cttttggcta tgctattggc
      601 gcgttctttg ccggcatatt ttttagtatc agtccccata tcaacttctg gttggtctcg
      661 ctatttggcg ctgtatttat gatgatcaac atgtgtttta aagataagga tcaccagtgc
      721 gtagcggcgg atgcgggagg ggtaaaaaaa gaggatttta tcgcagtttt caaggatcga
      781 aacttctggg ttttcgtcat atttattgtg gggacgtggt ctttctataa catttttgat
      841 caacaacttt ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt aggaacgcgc
      901 ctgtatggtt atctcaactc attccaggtg gtactcgaag cgctatgcat ggcgattatt
      961 cctttctttg tgaatcgggt agggccaaaa aatgcattac ttatcggtgt tgtgattatg
     1021 gcgttgcgta tcctttcctg cgcgctgttc gttaacccct ggattatttc attagtgaag
     1081 ctgttacatg ccattgaggt tccactttgt gtcatatccg tcttcaaata cagcgtggca
     1141 aattttgata agcgcctgtc gtcgacgatc tttctgattg gttttcaaat tgccagttcg
     1201 cttgggattg tgctgctttc aacgccgact gggatactct ttgaccacgc aggctaccag
     1261 acagttttct tcgcaatttc gggtattgtc tgcctaatgt tgctatttgg cattttcttc
     1321 ctgagtaaaa aacgcgagca aatagttatg gaaacgcctg taccttcagc aatatagacg
     1381 taaacttttc cggttgttgt cgatatctcc ctatccctca accggaaaat aataatacta
     1441 aagtgcttag cccggctaat aatcacctaa tccaaacgcc tcattcatgt tctggtacag
     1501 tcgctcaaat gtacttcaga tgcgcggttc gctgatttcc aggacattgt cgtcattcag
     1561 cgacctgtcc cgtgtatcac ggtcctgcga attcatcaag gaatgcattg cggagtgaag
     1621 tatcgagtca cgccatattt cgctatcagg attctgtgtg atggttacat cgcccggccc
     1681 agggctgttt agtcatcagc gctttctgac agtgctgaga tttcaacctg ttgcagtaaa
     1741 aatgagtaga tataaggcaa gtgtgctgcc aaacccatct tttacggggt gaaggtagat
     1801 ttcgtttgaa gggtatctgg tgtcccctgc agacatctac ttgaggcggc aggggattga
     1861 ttggaatgct gttttttaga tttgaaaaat attttacccg ctattttacc cattggcgcg
     1921 gcttaagagc ttatttttga attcacaatg gtcacgatat aaccatcttg ctcgtccgtg
     1981 gataactttg gctttaggca ggtcgccgga cttaatccgg tcatagatga aggtcttacc
     2041 gaagccagta tcagccatga tgaatttcaa atcaaccagt gaatcaggtt gtagttcgtg
     2101 ttgcatgagt gctatctccg aatatggaat cgaacctgca aatcaggcaa taaaaagccg
     2161 cattgatgca gcaatggtag gtctggatat cttgagaaat gaacaggcct catcgagtgt
     2221 gaggcgggtt agtccttgcg tagctcgctg attcttctgt aagtctctgg tgctttgttc
     2281 ccgtacgtct tcatttcaga cttcaacaga gcaacgagtg aatcccattc gttgaggatt
     2341 cctttgaatg ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc
     2401 tgctcaacaa gtgcaacgca tcgccgaaag tctgctgcgt catggagagc gccgaagtga
     2461 tgaccataga tattcttttt caggccacat gcgattgagg caagagttgc gctactgatg
     2521 ccgacatcgc cagttgattg ccatttcaaa accttcatag ccaaatctga catttcttgt
     2581 ctccataaaa caaaactcgc cgtagcgagc tcagataaaa gaaatccccg tcagtgcgag
     2641 gatgctgttc attgctgcta tacacttttt tgctctcaac gtaagcggta gcccattctg
     2701 ttgggttggt cagttgcttt taggaaatgc tctttacccc ttaaacgtcg gctgaaagag
     2761 ctaaaatcca tgcaaaaaat ttacgcaatt ttgtgtatta ttgtgcagta agtaatgagc
     2821 tattttctgt gcaaaaaatg gatgataaat ttgtccgggg caggaaaaat tttatgggcg
     2881 ctaaacatga aaaaagattc gtatccttat ttgatttgca tgacagtttc agggctgatc
     2941 tttattttcc ttttcttctg gtggcgggca gatatctaca gggtcacgtt tcttaatcag
     3001 agtatatccc actattacat tctgtttagc atgggaatag cttttctgtt atctctgttt
     3061 tgggttaaga aggggatagt aaaacaaagc ggctggaaga gtctgtcagc ataccttaag
     3121 gtttatgcag ggatatgcat attttctgga ttttttctga ttatacccct tacaacacta
     3181 acttattttt tgcctggaga gacatcgtct tatgttgcac cgtatcggta tacttccggt
     3241 agttcaaaaa gttgttctgg agctgaggtg gatgaccccg atctacatga gaatattcgc
     3301 atttgctatc cgtatggcaa ttatgagtac gataatatta tctatgttga aaagaaaatt
     3361 aatacattag gtgcggtagt gacatatgca cagaccgcgc gtgatgatac tgaatgagat
     3421 agtatatagc gggcaagttt tagttaattt atcgaggtaa tataatttac ctcgactcgt
     3481 ttattctggt atgaatattt cgctttacga tcgattttta tctgatgata tcatgcggtt
     3541 ttcatatact gacttactgt ct
//
LOCUS       DAJTXQ010000239         3537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000239 DAJTXQ010000000
VERSION     DAJTXQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.243"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..133)
                     /locus_tag="OO515_004107"
     CDS             complement(<1..133)
                     /locus_tag="OO515_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577879.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     "
     gene            complement(179..520)
                     /locus_tag="OO515_004108"
     CDS             complement(179..520)
                     /locus_tag="OO515_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577880.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(689..880)
                     /locus_tag="OO515_004109"
     CDS             complement(689..880)
                     /locus_tag="OO515_004109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577881.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(813..2144)
                     /gene="xylB"
                     /locus_tag="OO515_004110"
     CDS             complement(813..2144)
                     /gene="xylB"
                     /locus_tag="OO515_004110"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6577882.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2216..>3537)
                     /gene="xylA"
                     /locus_tag="OO515_004111"
     CDS             complement(2216..>3537)
                     /gene="xylA"
                     /locus_tag="OO515_004111"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6577883.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          921 a          981 c          781 g          854 t
ORIGIN      
        1 aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac agataggtaa
       61 cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa taggttgata
      121 ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt tatgttgctc
      181 ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg atggggtaga
      241 tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag agaccgatag
      301 tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc ccgagttttt
      361 ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca agaaaatagc
      421 cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc agcgccccga
      481 caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc atatctacca
      541 gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta acaaatgatt
      601 ttcagaataa attcatacta aatattaatt aattagtgag atatatagat gtgaattatc
      661 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
      721 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      781 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      841 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc cgtacggtaa
      901 tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact acgcgccccg
      961 ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac atccatgcca
     1021 tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt ggggccatgt
     1081 tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg tgtacgctcg
     1141 ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg ttgagctgca
     1201 gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc cagacacgat
     1261 gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg gcaaaagctg
     1321 tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata gacccccgac
     1381 gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac tgcaccagct
     1441 gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact
     1501 tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga
     1561 gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac atccagccac
     1621 atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagacgcaag
     1681 taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc atgccgctga
     1741 acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt aatcactcgt
     1801 gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac
     1861 aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc atgcatctgg
     1921 ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag agctttcatt
     1981 gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag tggatgcggg
     2041 cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc gttgagcaaa
     2101 ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa
     2161 aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt agccgttatt
     2221 tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca
     2281 ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt
     2341 tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg atgcgtttat
     2401 ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc atcgtatcca
     2461 tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg
     2521 catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca
     2581 gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc
     2641 cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat
     2701 ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta atctcttttt
     2761 ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca tattgatgtt
     2821 tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg attttatgtt
     2881 tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga cgcaagtcgg
     2941 tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca
     3001 atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg aagacttcag
     3061 gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag ttagcggttc
     3121 cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca accatttgcg
     3181 caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc acatcgtgga
     3241 agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct
     3301 tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc cccacaccaa
     3361 acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct
     3421 cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat gctaacgggt
     3481 ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgca
//
LOCUS       DAJTXQ010000240         3535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000240 DAJTXQ010000000
VERSION     DAJTXQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.244"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..295)
                     /gene="hspQ"
                     /locus_tag="OO515_004112"
     CDS             complement(<1..295)
                     /gene="hspQ"
                     /locus_tag="OO515_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6577884.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQL"
     gene            complement(353..1543)
                     /gene="rlmI"
                     /locus_tag="OO515_004113"
                     /pseudo
     CDS             complement(353..1543)
                     /gene="rlmI"
                     /locus_tag="OO515_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            1638..1916
                     /gene="yccX"
                     /locus_tag="OO515_004114"
     CDS             1638..1916
                     /gene="yccX"
                     /locus_tag="OO515_004114"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6577885.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(1913..2242)
                     /gene="tusE"
                     /locus_tag="OO515_004115"
     CDS             complement(1913..2242)
                     /gene="tusE"
                     /locus_tag="OO515_004115"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6577886.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2333..2992)
                     /gene="yccA"
                     /locus_tag="OO515_004116"
     CDS             complement(2333..2992)
                     /gene="yccA"
                     /locus_tag="OO515_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6577887.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3199..3286)
                     /locus_tag="OO515_004117"
     tRNA            complement(3199..3286)
                     /locus_tag="OO515_004117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3250..3252),aa:Ser,seq:tga)
BASE COUNT          956 a          905 c          848 g          826 t
ORIGIN      
        1 ggagttgttt gcggatggtc tgcgccagct catccatgga tggctgttca ggatgttcat
       61 cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt aggccgttat
      121 cgtcctccat cactacgtga taccacggag cggcgcgaag ctcgtcattt accgccagct
      181 catcaggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc actccgagat
      241 aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca atcatagtca
      301 cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca agttacatga
      361 cgcgacaggc aaaccctttc agatatagcc cttccggata ggtagcgatc accggatgat
      421 cggctgcctg acggaactgc tctataaatt gtacatcacg gccggcatca attgcggcat
      481 ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag aaagtcagga
      541 gaataccgcc ttcattcagc aactgaatcg ccagcatgtt gatatcttta tagccacgac
      601 acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc acgataacgt
      661 caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca tcacggacaa
      721 actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca atatccagcg
      781 cttcctagga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt gccgatacgg
      841 cgaaaccacc ggtataggag aaacagttca gcacacgttt attttcaacg tagcggcggg
      901 tagccaggcg gctgtctcgc tggtccaggt agtagcccgt tttgtgtccg tgctgaatat
      961 ccaccagcag cttcatgccg tgttcttcaa tcggcagcag ggcaggcggc aactcgccgg
     1021 tgacgaggcc ctgggtcagc tccatccctt cttttttacg taccgcaaca tcgctgcgat
     1081 cgtaaatcgc acattccggg tacagcgttt gcagggcact aattaatgcc gcgcgctgat
     1141 attctgcccc agcactcagc agttgcagca ccagaaaatt accgaaacga tcgatagtaa
     1201 tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg ccatcttttt
     1261 gcgccagtca gtcacgccat ttttgtgctt gttgcaaacg gcgggaaaaa aaagcaatgt
     1321 cgatagactc agacgggtca aacgtccaga cgcgcgcccg gatttgcgaa gctggcgaat
     1381 aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt tcaccgaggc
     1441 tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg cgacgaagta
     1501 atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac ttttctgtcg
     1561 atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg cgtccattaa
     1621 ggataaggag agcagcaatg tcgaaagtct gcataattgc ctgggtttac gggcgagttc
     1681 agggcgtagg atttcgctac accacacagt acgaagcgaa aagactgggt ttaaccgggt
     1741 acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ctgcggtgaa gaagggcagg
     1801 tggaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt gtagaacggg
     1861 tgctcagcga gccgcatcat ccctcggggg aattaaccga ttttcgtatc cgttaaatac
     1921 attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccgga cctttcggaa
     1981 acagtcggta taaatagcgg ctgttacctt tttcttcgcc aaatttattc gccatcgctt
     2041 ttaccagcat acgaatcgcc ggagaagtat tgaattccag atagaaatca cgcacaaaac
     2101 gcaccacttc ccagtgttcc ggtgacagcg aaatcccttc gttctctgca atcaccactg
     2161 ccagcggctc actccactgg ctgctttctt tgagatagcc ttcggtatcc gtttctatct
     2221 ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg gcagtgtaac
     2281 aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtga gattaatcgc
     2341 ggctggcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc gaaacataca
     2401 ggctaaccgt ggcacgaata tagttcgttt caccgccatg aacgatgttg ctggtttcaa
     2461 acaaaatagc gccagaggag atcagaatga agaccgcgct gatcgccaga tgcagagcag
     2521 gcagctgcag gaagatatta gcaaccatac caatcagcac caccacaata cccgccatca
     2581 gcataccgcc gaggaacgac atatctttgc gggtggtcag cacatatgca gagcagcaga
     2641 agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt ccggcagaca
     2701 gataggtgtt cagaataggt ccgaggatat aacccagaaa accggtaaag gcgaatgcgg
     2761 agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa ccatacatac
     2821 ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg gcagttgcgg
     2881 taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc agcaccttat
     2941 gggtgctaag cagtgatgta cggtcatgtg aagaactaac aatacgatcc attgagtcac
     3001 tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg gcacccagaa
     3061 gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt atcaggtcat
     3121 taagcaaata taacgccctg agaatttcga caggcaaaag aaaaaggggt tagcatttag
     3181 ctaacccctt atcttatttg gcggaagcgc agagattcga actctggaac cctttcgggt
     3241 cgccggtttt caagaccggt gccttcaacc gctcggccac acttccggaa tgacgcgcac
     3301 tataaacatc ccgatgcggc gtgtaaaccc ctaatttgtt tgtttgcctg aaaaacagcc
     3361 aaaagtgcat tgatagcgtg aaataacagc agattgatca tttcatcacc atgaattcct
     3421 tctcttttac tcgtttagca accggctaaa catccccacc gcccggcaaa aagaaaaata
     3481 ggtccatttt tatcgctaaa agataaatcc acacagtttg tattgttttg tgcaa
//
LOCUS       DAJTXQ010000241         3525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000241 DAJTXQ010000000
VERSION     DAJTXQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.245"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(263..463)
                     /locus_tag="OO515_004118"
     CDS             complement(263..463)
                     /locus_tag="OO515_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577888.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="OO515_004119"
     CDS             complement(463..735)
                     /locus_tag="OO515_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6577889.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="OO515_004120"
     CDS             complement(738..1016)
                     /locus_tag="OO515_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577890.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="OO515_004121"
     CDS             complement(1017..1574)
                     /locus_tag="OO515_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577891.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..>3525)
                     /locus_tag="OO515_004122"
     CDS             complement(3138..>3525)
                     /locus_tag="OO515_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577892.1"
                     /translation="AYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREG
                     FDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTL
                     ISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          976 a          734 c          651 g         1164 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcctg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttgcaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccggaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aacccttctc taaggctgat aactgtcaca
     3421 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
     3481 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagct
//
LOCUS       DAJTXQ010000242         3496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000242 DAJTXQ010000000
VERSION     DAJTXQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.246"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1513)
                     /locus_tag="OO515_004123"
     CDS             complement(<1..1513)
                     /locus_tag="OO515_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6577893.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFC"
     gene            complement(1510..1809)
                     /locus_tag="OO515_004124"
     CDS             complement(1510..1809)
                     /locus_tag="OO515_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6577894.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1816..2136)
                     /locus_tag="OO515_004125"
     CDS             complement(1816..2136)
                     /locus_tag="OO515_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577895.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2129..2326)
                     /locus_tag="OO515_004126"
     CDS             complement(2129..2326)
                     /locus_tag="OO515_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6577896.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2380..2832
                     /locus_tag="OO515_004127"
     CDS             2380..2832
                     /locus_tag="OO515_004127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577897.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2890..3066
                     /locus_tag="OO515_004128"
     CDS             2890..3066
                     /locus_tag="OO515_004128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577898.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3178..3387)
                     /locus_tag="OO515_004129"
     CDS             complement(3178..3387)
                     /locus_tag="OO515_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6577899.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          876 a          938 c          741 g          941 t
ORIGIN      
        1 cgcagaagtc caccagcgaa tcgccctcgc gttttatcac cagagcttct tctgatttct
       61 gctgctcata cagcaggcgt ttagcttcgt cctggtcaga aaaacgagta agcaggtggc
      121 gaatcacaac cgccagctct ccttctattt tttctgccag catggggtcg cgttcgttct
      181 ccggtacaac ttccgaaaaa ttgaatatca cccgacgacg tgagatcccc ccgctgcggt
      241 cactgaatga catggcgtta ttgttaaccg ccagcactac tgccggaata cgcgttgagt
      301 agggggcttt gtgtttcggg tcaattgcca ccttgtcacc gcctgtaatg gccttaatcc
      361 ctgccccatc accagcgtag cgggtcatat ccggcatgat aatcagcgaa aagccaacca
      421 ctaacgcgcg ttcccttgca tcttccagcg ccttcatgct tgccgatact gtgttggcct
      481 tacccgccag catggtgcaa atctccgcca tcacgctttt accacttccc cctggacctg
      541 ttacctcaat gaataactgc cagtcgtacc ggttcgccag caccatgaat aatacagcca
      601 gtacgcgatc cgccttgcgg tcattctcag ccaccgaacg gcgtaaccac ttccagaaat
      661 tcggcgcatg tgttgccagc gtttccccct ctgctggtgg gctgaaaggt aattcactgg
      721 caattaacaa ccagtcgttt ttgttatgct cccgaaaatt acctgttctg gtatcaaata
      781 ccccgttact gaatccaatc aggttacggg ctgtattccc cattacaggc aaacttaact
      841 tcatggtatc gaccgccgat ttaatggcgt tctgcgaata gctgatctcc gcatcaatga
      901 aaatctgtgc catagctcgc tgtaattctt tatcctgtac tggctcccat acaacgccgt
      961 tgtaatggtg aacagtgtca gagtcagcat gaatcgccag ttcaccgcca taatgtgcca
     1021 ggagaacttc gccgcgttga cttgctccca tctggttaag cgccagtgat gaagcgttat
     1081 cgtcttttac ccgctctttt ttctttacag gcagttcaac tacctttttc ttttccgcct
     1141 gctctgcccg ttcacgttcc agatattcgc gccagttctc ccgtttctgg ctgtgcattc
     1201 cttcagggta ataatcagca tccctgacac ctgccgctgc cagtttctgc ccgatggtat
     1261 taacaagccc cggacgcaat aaccccgcct ggtagagacg cacccgatag cgtccgtccg
     1321 gtacgatttg cagcttatcc agttcggcaa gttgttgttc tccaagccag acaggaggca
     1381 cgctgtcgcc agccagtcgc ccgtcctgtt cctgccactg cttcgcatgt gcccacgcat
     1441 cattacccgc aaaaatgatg acttccgtca ttttgtcacg cggctggtgt tttaaatttg
     1501 gcgctttttt catttctgct ctctccacgc ggcaatcatg tttttcagtt cctgtagttt
     1561 tttatcaaca tccatacatg acacatggtt atttctggaa agcgggattt cccgcctgaa
     1621 tctgctaata aagatctcca cgttcagcga actatgaaat gaatagccat cacgaataaa
     1681 atacacacgg tcaaacatca gttcttttac cgttactctg ttaccgttct tatccagata
     1741 aatagcgccg gggataattt tggggtgtgc ataaccgctg gcagtcaagc cagataaata
     1801 cgttctcatg attatttatc tcagatttga atcagtattc gctttctcta tggcatttaa
     1861 tgcatctgtg gcattttcaa tggtgcaccg taacgaaata tcaaactgcc caagcattgc
     1921 cagtaacaag ccaatattac ccatgtcaat gcgcatagcc ttttcgtcat attcctcatt
     1981 ttctgacgca tgccacatca ggctaccaat tgacgcaaca gccattgata tattgtcagt
     2041 agccccatcc gcagcggaat aaaccttttt agcaatatca tgctcacagt taaaatgcgg
     2101 attaatcagg tactggtaat tggtcatgtc aggcatggca cacctcctga cgaatacggg
     2161 cggcgaatac agcaacacaa cctgacgggc aacggttacg cgcttcgcgt tccgtccagg
     2221 cggttacgtg gatgatttga gattctccgg cactcagggc cagaaaacgc cacacaaagg
     2281 ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg gctcctcgtt
     2341 tacgttgttg gttagaagcc ctgcgagtgg taacgacact tgcggggctt cgtcgtttca
     2401 attgctgaat tgcatgtatc agcacttgtg gtattcacat tacatttagg tgaataccat
     2461 ttcaagtctt ttttggtatt cacttttgta ttacactgca tcccgttatt aatgggaggt
     2521 gcaaacatgt ccaggagttc tgttaacaat aagtcacagc aactgaatgc cagatttcca
     2581 catgaagtag tgagtggcat tgaggcatcc ctacagccag gggaaactaa agcgaatttt
     2641 atagttacgg ctgtacgcgg tgagatcgcc aggcgccagg cagaaggaag tggagaaaat
     2701 ccccttgtgt cttcactgga tgccctagct aatgtcgaac aaatcggcat caaggcagcg
     2761 gaggaaatcg ggcagcttat caccgttgca cgcgaagaac tccagcgccg caaggccaaa
     2821 gaatcagaat aatcactatc agcgccgtgg tgtgaggaac tccggcgcat tgctttacag
     2881 gcgcacacaa tgaccaacga agaatcaacc aaaaaaacat cacctgcacg aaaaagacga
     2941 cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc ccttgagaag
     3001 taccttaaag aatactcatc aaaggagaga gcttcgcagt tatggcaacg cactgaaaca
     3061 gactgatagc attgcccacc agcctgatag cggctatcat tgacatgctt atgtttagtg
     3121 tttccaaact ggcgaccgcc ccaggtcgcc tttgttttat gtgtcatatg ctccccttta
     3181 cactgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa cctcggacag
     3241 cagccaccct acagaacgac cgcccagatt aagacgtgac ggaaagcgcc cttttttctc
     3301 cagttcgtaa cgtgtagtgc ggcacactcc agttaactta cgacattcat cctcacggat
     3361 tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac gtatataaac
     3421 ttttctctta gctgtagata tatgagatcg gatattactt agattctttt tcacctctta
     3481 aattaaaaac acaacc
//
LOCUS       DAJTXQ010000243         3318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000243 DAJTXQ010000000
VERSION     DAJTXQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.249"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO515_004130"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO515_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6577900.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO515_004131"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO515_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6577901.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="OO515_004132"
     CDS             complement(1251..2825)
                     /locus_tag="OO515_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6577902.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3318
                     /locus_tag="OO515_004133"
                     /pseudo
     CDS             3080..3318
                     /locus_tag="OO515_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          849 a          790 c          711 g          968 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatg
//
LOCUS       DAJTXQ010000244         3207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000244 DAJTXQ010000000
VERSION     DAJTXQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.250"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..1380)
                     /locus_tag="OO515_004134"
     CDS             complement(61..1380)
                     /locus_tag="OO515_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6577903.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1511..3046)
                     /gene="gadC"
                     /locus_tag="OO515_004135"
     CDS             complement(1511..3046)
                     /gene="gadC"
                     /locus_tag="OO515_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6577904.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT          847 a          792 c          796 g          772 t
ORIGIN      
        1 cacaaggtgt aacaagccgg tgggtaaagc accggcttgt tacaaagtaa gcatgggagt
       61 ttaactgccc caacgacttt gcagatagct gaccgcttgt tgagtctgcg gtttattcag
      121 atagtcctca cggaacaaga tggtgccgct aatttctggt accgcatcgt taagatcgag
      181 ctgctttttc agttccggta cgccgccgtt aatcatccag tctggctcta tctttgaagg
      241 ttcacccact ttatagaagg cgataccgat atacaggcgg gtcctagtcg gtttaacgac
      301 atccgcccac cattttgcca acacgtcata acgcgcggca ctccgtgaga acggccagta
      361 aatttgggga gcaatgtaat ccagcaatcc ttgttccacc catcgacggg tgtcagcgta
      421 ggattcgtca taggctgccg cgcctcgggt atcggaaccg agcggatcgt gtgatcggtt
      481 acgccacacg cctgccgggc taacgccaaa ttcgactccc ggcttaatgc ttttaatggt
      541 gtgcgatact tttgcaatta actgctgagt attgttgcgc cgccagtctg cttttgacgc
      601 aaatgcgcct ccgtatttgc ggtacgtttc gttatcattt agccgtgaac ccggtgactc
      661 agtatagaaa tagtcgtcaa actgcacgcc atctaccgga tagcgggaaa ccacttctgc
      721 gactattgat gtgatccagt cctgaacctc agggatgccc gggtcgagga caaagcgatc
      781 gccagacgtt ctgatccagt cacggtgttg cacatagacg ctcgccggtt gttgagacag
      841 agtgctattc agttccctga tagtaccggg cttcgtatta accgatacgc gataggggtt
      901 aaaccaggcg tgtactttca tcccacgctt gtgggcttcg tcgagcataa attgcagcgg
      961 atcgtaaccc ggattttcac caatcttacc ggtcataaga tcggaccatg gcaaaatttt
     1021 cgatggccac agggcggtac cgtccggctt aacctggaaa aagaccgtgt ttataccgag
     1081 acgttgcaga tgatccagtt tgtcgatcat cgcctgttgt tgtacacggg cccggctggt
     1141 ggggttacta atgttaaccg aggaaaccgg tggccagtcg aggcgagaca ccgtggccag
     1201 ccagatgcca cgcatcggtt gtgacgattg ttgcgtcgtg gctggtggct ttgaacctgc
     1261 tggtggtgtc accatggact ctggaggcgt gcttttacaa ctacacagta aaagtgccag
     1321 tgcaaccagt atcgctggtc ttctaatcgc taatttctcg tttcgggagc agatatccat
     1381 atatgctggt ttccggtaaa cagatgtgcg ctcattctcg tacttatccc ggttaagtca
     1441 atacgacagc gagcacgaaa aagggagcga tgaattatcg ctcccttgtc ttataaccat
     1501 tcagacatgg ttagtgtttc ttgtcattca tcacaatata gtgtggtgaa cgtgcacgcg
     1561 ggtgcaggaa gaagtgacct tttggtgcgt tctgactgtt gattggctcc agagtgacgc
     1621 cggtattcgc tttgccttta cgatcatgaa cagcatagag aataaagggc agggcaagta
     1681 ccaccaggaa actaacgacc agtaattcaa catacatatc ggtagagtca ccctggatgt
     1741 tatccggcgg caggaaggaa acaataaacg ccataattga agtcagcaga ccgacaatag
     1801 ccacgaccag tttcacccct ttaccaccag ggatattaaa tgtgcgtttt aagtcaggat
     1861 gtttaagaac caacacaatg tagccaataa acagcatgaa ataagcacac agataaatca
     1921 ccaccgtcag cgccagtgcg atcaggaagg acatgttgtt accgccaccg gtattggtga
     1981 ggatgatcaa cgcgatagaa gtaatcacca gctgtgaaat gaccagcgtt accggtacgc
     2041 catttttgtt cattttagcg aatgccgctg gcagcaggtt tttctgcgct gtgacataca
     2101 tcccgcgaga aggaccaaca atccaggagg cgatttccgc cagaacaccc agcaacagca
     2161 gtgcggagat cacgcgaacc gtccactcaa tttccggtgc cacatgggac atcagaacgg
     2221 taaaggtttg cattacccct gcggagaggt tgatttcatt acccggaatg accatcgcaa
     2281 tagacaaacc accaacagag cttaagcaga ttgccgctac catcagcagt aacatagcca
     2341 acggatagtc gcgccctggg ttgctcattt cattgacgtg ggttgcggat gcttctacgc
     2401 ccatataact caaaatgaag gcaacaaata caaccagggt gcccacttta gagaagtcag
     2461 ggaagaaggt ctttgaatcc atttcgatag caacgggggc accggagtgc agataaatag
     2521 ccgctaatgc gatcaaaata aatgcaggta acaggatacc ggcgaagaag ccaactttag
     2581 caattcgtgc cgtgtatttc gtgccaccaa actgcgttaa tgccagcgcc caaagaatga
     2641 tgagtgctgc aatagttttg gtaatggggt cttcattcag cgctggccat ttcaggatgt
     2701 aggagagtgc ccctaacacg aaatagagca tcggaataaa accaatggcg atttgcagat
     2761 agccaaatga gatcgctgca aatccccatc tcggccccag agtatttgat acccaggcga
     2821 agacaccacc ttcttcccag ccgtcgacgg tggccatttc cgcagcacaa agtcccacgg
     2881 gaataaacca taaaatcccg cctaatagca ggaagaagac taatgaaaag cccgatgttg
     2941 cgaaggtagg gtattcataa acagccatta ccatcgatgc cgttatggca aaaaagccga
     3001 gtaatgtgag ctgcttagct ttacctgtct gtactgatgt agccatatta tccccctaaa
     3061 acggtattcc tgtcggaacc gcacctgttt tgttctgaag cgtattcaga acaatatttt
     3121 ccgttgctaa tgccagtgaa cagactttgg aaattatccc gaaacgggtt cgtttcggga
     3181 caccgttacc gttaaacgtt atcaggt
//
LOCUS       DAJTXQ010000245         3196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000245 DAJTXQ010000000
VERSION     DAJTXQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.251"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO515_004136"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="OO515_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6577905.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO515_004137"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="OO515_004137"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6577906.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="OO515_004138"
     CDS             complement(1715..1984)
                     /locus_tag="OO515_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6577907.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="OO515_004139"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="OO515_004139"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6577908.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="OO515_004140"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="OO515_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6577909.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          833 a          774 c          771 g          818 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatccggca tttttt
//
LOCUS       DAJTXQ010000246         3151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000246 DAJTXQ010000000
VERSION     DAJTXQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.252"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            165..755
                     /locus_tag="OO515_004141"
     CDS             165..755
                     /locus_tag="OO515_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6577910.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            742..1782
                     /locus_tag="OO515_004142"
     CDS             742..1782
                     /locus_tag="OO515_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6577911.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            1785..2330
                     /locus_tag="OO515_004143"
                     /pseudo
     CDS             1785..2330
                     /locus_tag="OO515_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2354..>3151
                     /locus_tag="OO515_004144"
     CDS             2354..>3151
                     /locus_tag="OO515_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6577912.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDA"
BASE COUNT          804 a          709 c          909 g          729 t
ORIGIN      
        1 gggcgtatgt atctgtccag aatcagtgag gccatcagtt tgtctgctgg tgaagtggcc
       61 caccggttaa tcgtcccttc atcggatatt gacctggggg agaccgagct tccggtgctc
      121 ggcgagatca cctggcaggc ttatgacccg gcaaggagta aataatggat tcgttacagg
      181 atgattatac aaagctgctg tacggactga tgccgccggg gcctgcatgg tcagataccg
      241 acggtgtact tgacggtctg gcaccatcgc ttgtgcgtgt tcaccagcgg gctgatgaac
      301 tggtgattga aatcgatccc ggtcagtcca cagagctgat tgaacgttat gaagaattgt
      361 atggtttacc tgattcctgt tcccctgtcg gaacccagac attacgccag cgtcagcaac
      421 gtcttgaagc aaaagccaat gtggctggtg gcataaatga gcagttcttc ctggatcagc
      481 ttgaggcgct gggatatacc ggcgtaacga tcgaacagtt ccagcacttg gatgcaagcc
      541 ccgatccgga atggggagat cgctggcgtt atttctggcg tgtgacgttg ccgatggatg
      601 ccggtgctca gtggcagaca tgcacggacg cctgcaacac accgatccgg acgtgggggg
      661 atacggttgc ggagtgcgtg attaataaat tatgtccgtc acacaccgtc gttttatttt
      721 cctatccaga tgaggatgaa gatgcacagg attgatacgc tgactgcggt aaaagataag
      781 tttggtccgg gtaagaacgg atttactgac ggaaatcttc gcacaggacg tcttgctacc
      841 tggctgaaca gtgccatgtg gaatgccatt caggaggaaa tctgtggtgt cattgagaag
      901 gccgggatag aactgaataa agaagaacac gaccagctat ataaggctat attattgctg
      961 gtgggcggtg caattaacga agaggcattg ctgataaaaa ataacctttc ggatgtggaa
     1021 gacagggatg aggctgttga aaacctcgga ttaaaaccca cggtggataa ggcaaaaaat
     1081 gccgttcagc gtgatggcga caccatgacc ggagagctga aaatccgtgg tgttaatgcg
     1141 ctgaggattt tcaacgaagc ttttggcctg atttttcgtc gttcggaaga gtgcctgcac
     1201 cttattccca ccagtgaagg tcagggggaa aatggcgata ttggtcccct gcgcccgttc
     1261 accattaatc tgcggacggg tgaaatatcc atgtcgcata aagtgtctgt tggcggtggt
     1321 tcgcaggtca atggtgcgct gggtatcggc gttcagaacg ccctgggggg gaattcaatt
     1381 gttcttggtg ataatgacac cggattcaaa cagaatggtg atggcctgct ggatgtttat
     1441 gccaatagcg tgcatgtgtt gcgttttcag agtggcagta tccagagtaa taaagctgta
     1501 aacgttacag gacgggtaac accgtcagac tacggaaact ttgatgcccg ttaccagcag
     1561 cgaaatggtg gcgtgcagga tgtgcggtat ggttatgaaa tgtattaccc ccccggcagt
     1621 aacaccgttt cgtggacatt tcgctcacct tcgggacacg ggctgtcagg gatatcgata
     1681 tcggataccg gccgtaactc agcggataac gtcaacggcg tgtattaccg accgctgcaa
     1741 aaactgatta atggcacctg gtataacgta gcgagtattt aacaatgttg catttaaaaa
     1801 atattacggc aggtaatccg aaaaccgcgg aataatatca gctgacaaaa cagtatgatg
     1861 tcacctggct tttttcggaa gacggcaaaa actggtatga agaacagaag aactttgcca
     1921 gtgacaccat aaaaatggtt tacaccggag acgggcgcgt ggtgtgggtc ggtaaggatg
     1981 tgacaggcat tgaaccccgt aacgccagtg ttattgaagt tcctgatatt accgccaacc
     2041 gccgcattac cgcgccgggt tactggtttt accgcaatga tgaatttgtc tttgactaca
     2101 gactcaaagc ggaagatgag cgtgatgccc ttctggctca ggtcagtgcc cggacagggg
     2161 aatgggaaga agacctgctg ctggggctaa tcagcgacga agaccgggaa aaactgaaag
     2221 cgtaccgtat ttacgcgaaa tcgctgcagg cgatggattt cagcaccatc actgataaat
     2281 cctcatataa cgccattgaa tggtccgtct ctccggaagg ttcttcctga tttaatttat
     2341 cgcgagaaaa actatgtctg tagtgatatc aggtgcgctg actgatggcg caggcatccc
     2401 catgtccgga tgccagatta ttctgaaatc ccgtgtaaac acctcagaag tggtgatgcg
     2461 taccagggct gatgtagtga ccggaaataa cggtgaatat tcgtttgagg cacaggtcgg
     2521 aaaatattgc gtgtatctga aacgggactg gcgcgacgag tactgtgttg gcgacattgc
     2581 tgtatacgac gactcaaagc ccggcacgct gaatgacttt ctgattgctc ctgatgaggg
     2641 cgacctgaaa ccggatgtcg tcaaacgctt tgaggaaatg gtggcgcagg cgcagcagag
     2701 cgccggggcc gcagccggaa acgcacagca gacggcgcag gatgtggcgg cagccgcagg
     2761 ttatgcccgc gcagcagagc aggccaaaaa tgacattgat gctgcgctga ccggcactct
     2821 gaaaacggct aaccatctgt ctgaaatcgc agcagcaggc gaaaaggcac aacagaagtc
     2881 ccgggataat ctggggctga aaagtgcggc cacgatggaa gcacagagcg acatttacga
     2941 ccggacaaaa ggccgtctgg cgatacccgg cgcattcggc tttgggtgtg cttttctgcc
     3001 tgaagatgtt atccgttttg acactaagag tgatttcctg gcctgggtaa ggaatgcgct
     3061 gccaggtgaa tattccgttg ctggccccta cgacatcatc atacccgaca cacggtttga
     3121 aggggtgctc agcatccggt ggactgatgc a
//
LOCUS       DAJTXQ010000247         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000247 DAJTXQ010000000
VERSION     DAJTXQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.253"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1258)
                     /locus_tag="OO515_004145"
     CDS             complement(<1..1258)
                     /locus_tag="OO515_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6577913.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="OO515_004146"
     CDS             complement(1415..1624)
                     /locus_tag="OO515_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577914.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="OO515_004147"
     CDS             complement(1647..2357)
                     /locus_tag="OO515_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6577915.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="OO515_004148"
     CDS             complement(2873..>3112)
                     /locus_tag="OO515_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577916.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          654 c          507 g         1038 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcca
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAJTXQ010000248         3010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000248 DAJTXQ010000000
VERSION     DAJTXQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.254"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..1226
                     /locus_tag="OO515_004149"
     CDS             63..1226
                     /locus_tag="OO515_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6577917.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OO515_004150"
     CDS             complement(1686..2123)
                     /locus_tag="OO515_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577918.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3010)
                     /locus_tag="OO515_004151"
     CDS             complement(2212..>3010)
                     /locus_tag="OO515_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6577919.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          794 a          649 c          674 g          893 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaatta ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc
//
LOCUS       DAJTXQ010000249         2992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000249 DAJTXQ010000000
VERSION     DAJTXQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.255"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..1076
                     /locus_tag="OO515_004152"
     CDS             18..1076
                     /locus_tag="OO515_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6577920.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="OO515_004153"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="OO515_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6577921.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="OO515_004154"
     CDS             2805..>2992
                     /locus_tag="OO515_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577922.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAJTXQ010000250         2955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000250 DAJTXQ010000000
VERSION     DAJTXQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.256"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..986
                     /locus_tag="OO515_004155"
     CDS             <1..986
                     /locus_tag="OO515_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6577923.1"
                     /translation="ESGELSFPVAGAATARKLEESSVFLDELIEDMFGDFDLAGIPDF
                     IQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDLSVILMPPSVASDFVHTLQDT
                     WRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPTDSASVIRQKQQTNLVAAVIR
                     TTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQSDIIHVSHPALDSVAASTGQD
                     ERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQLTSLRTELNRDIQARLVQTEE
                     TAERTPAEVLPALVLAASWYDDASRETDILDRNAISHPGFVPVRALRVPVR"
     gene            983..2065
                     /locus_tag="OO515_004156"
     CDS             983..2065
                     /locus_tag="OO515_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6577924.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            2043..2576
                     /locus_tag="OO515_004157"
     CDS             2043..2576
                     /locus_tag="OO515_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6577925.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            2582..>2955
                     /locus_tag="OO515_004158"
     CDS             2582..>2955
                     /locus_tag="OO515_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6577926.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHN"
BASE COUNT          670 a          726 c          884 g          675 t
ORIGIN      
        1 ttgagagcgg ggaactgtca tttccggtcg ccggagctgc aaccgccaga aaactggagg
       61 agtcgtccgt attcctggat gagttgattg aagacatgtt cggcgatttt gatctcgcgg
      121 gaataccgga ctttattcag aacgatgtta ttgcccggac gaccgatatg ctgggaaccg
      181 ttcagacagc tttcaaaatg gttaattcgg ctgtttctgc cggagcgaga ctactgcagg
      241 gcgatttatc cgtcattctg atgccgccgt cggttgccag tgattttgtg catacgttgc
      301 aggatacctg gcgggccgga acccggctgg tggataacac acaggatctg gtgcagtcca
      361 taacgacaat gtccggcatt acgctggacc caggactggc accgcgtgcg gtgtggccca
      421 cagattccgc atcggttatc aggcaaaaac agcagacaaa ccttgtggct gctgtcatcc
      481 ggacgacggc aatcagcgaa gccacaaggg cggtcacttc actgccacaa cccggaagcc
      541 tggtgaaaaa tcagcaggcg gttgtggctg ttggtggttc cactgaacgt cagtccgata
      601 ttattcacgt ttctcatccg gcacttgaca gcgtggcagc cagcacagga caggatgaga
      661 gagcgcaacc acccacgcgg gaaaatctca ccatcatacg caaatcgctg aacgcggcga
      721 ttgaacagga gctcaggcgt acggcggatg acaggctgtt ttttcagctg acgtcattac
      781 gtacagaact gaaccgggat attcaggcgc gtctggttca gacggaggaa accgcagagc
      841 gaacgccagc ggaagttctg cctgcgcttg ttctggctgc gtcatggtac gacgatgcgt
      901 cccgtgaaac tgatatcctg gatcgaaatg ccatctctca tccgggcttt gttccggtca
      961 gggcattaag ggtacccgtc agatgaatga taccgtttta cttcgggttt ccggtcgcga
     1021 atggggcggc tggacatccg tccgtatcag tgcgggcatt aaccgtattg cccgggattt
     1081 taatgttgcc attaccaccc gctggcccgg aagccgggat tatcaacccc ggataaaaaa
     1141 tggtgagctg gttgaagtgc ttatcgggga tgagcctgtg ctcaccggat atgtggaggc
     1201 actaccgctt cgttatgacg ccagcagcgt cagcatggga attgtcgggc gaagcaaaac
     1261 agccgatctg gttgactgct ctgctttgcc actccagcag agcggaaaaa acctgctcag
     1321 aatagtcagt gaactggctg cgccatttgg catcaccgtt gttgatgctg gcgtgccgca
     1381 gacagcggtg attgatgccc agccggaaca cggcgaaacc gttgccgatt gtcttaaccg
     1441 gttactgggg caggttcaga cgctggctta tgacgatgaa tgcgggcgac tggttctggg
     1501 aaaacccgga acaggtaaag cggcgacggc gctggtgctg gtgctgggag agaatattct
     1561 ttcctgtgac acggaaagaa gcatcagaga gcggttttct gaatatcagg tcagtgggca
     1621 gcgcccgggc aacgacgatg attttggtga ggccaccatt gccgcaatac gtcagaccat
     1681 tcaggacagt ggcgtgaccc gttatcgccc tttgttgatt cagcagtcag gcacagcaac
     1741 gacagcaacc tgtaaggccc gttgtgaatt tgaagcgcgc caacgggctg cgcttacccg
     1801 tgagacaaca tatacggttc agggctggcg gcagggcagt ggcgcgttat ggcgtccggg
     1861 gttatctgtc atcgttttcg acccgctgaa taattttgat aatgatgaac tggtgatcgc
     1921 agaagttacc tataaccagg acgaccgggg cacgacgact gaattacggg ttggcccggc
     1981 agatgcttat ctccccgagc ctgttaccgc caggaagaaa aaaatgttga ggaggatttc
     2041 tgatgaacgg gttttctctt cgtaatctga tttcccgggc tgtcatcacg gcggtggatt
     2101 ccgccagaaa gtgtcagtct gtagggttga aaatgatagc cggagatcag aaacaacacg
     2161 ttgagcacct tgaaccttat ggttttacat ctgccgcaca gaacggtgct gagggcgttg
     2221 ctttatttcc ggcgggcgat cgttctcatg gtgtggttgt ggtcgtggct gacagacgtt
     2281 accggctgaa aggactgaaa cgtggggaag tggcgcttta tgacgatctg ggacagtccg
     2341 ttgtcctgac ccgttccggt attgtggtgg acggggccgg gaaggccata atttttaaaa
     2401 acgcgcctaa agcgcgcttt gaaatgccag tcgaatccac cgccgatatt actgacaatt
     2461 gcgacagtgg cggactcagc atgcagcaaa tgcggcaggc ctacaatgcc cacaaacata
     2521 ccgaaaatgg tgatggtggc gggatcactg acacgccgga tcaaccgatg ggctgaaaat
     2581 catgatgatt aatgttaacg ggcgacccgt gtcgaccggg gcttcgatcg accttctgac
     2641 gcgtgctgtg attatttcgc tttttacatg gcggcgtgcc gggcgtgatg atgcaccacg
     2701 gatatttgga tggtgggggg atacctggcc tgcggttcag aatgatcgca cggggtcgcg
     2761 tttgtatctg ttgcgacgca gcaagctgac aaataaaacc ccgcagcttg ccagagatta
     2821 tgtccgtgag gcgctggcgt ggatggtgga ggatggtgtt gcttcccgtc ttgatattaa
     2881 cgctgtccgg accgggacag actcgctggc acttgccatt accatttacc agcgtgacgg
     2941 caatattcac aacat
//
LOCUS       DAJTXQ010000251         2936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000251 DAJTXQ010000000
VERSION     DAJTXQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.257"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..262
                     /locus_tag="OO515_004159"
     CDS             35..262
                     /locus_tag="OO515_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6577927.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OO515_004160"
     CDS             complement(263..637)
                     /locus_tag="OO515_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6577928.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO515_004161"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO515_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OO515_004162"
     CDS             complement(2097..2672)
                     /locus_tag="OO515_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577929.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttc
//
LOCUS       DAJTXQ010000252         2833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000252 DAJTXQ010000000
VERSION     DAJTXQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.258"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(105..419)
                     /locus_tag="OO515_004163"
     CDS             complement(105..419)
                     /locus_tag="OO515_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6577930.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(424..639)
                     /locus_tag="OO515_004164"
     CDS             complement(424..639)
                     /locus_tag="OO515_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6577931.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(790..2643)
                     /locus_tag="OO515_004165"
     CDS             complement(790..2643)
                     /locus_tag="OO515_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6577932.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
BASE COUNT          671 a          786 c          676 g          700 t
ORIGIN      
        1 tcagcgccag aacagcagct gacaggccgt atttgatttt tgcgctcatg gatatttatc
       61 aggatgctac caatgaaaga tactggaaag ccaactgcaa aaagctaaca acccgtaatc
      121 gagttatcag aactgttaat ttttatggta taccgcgcct ctgaacaggg gcgcgtttct
      181 ggcaacagct cgtccccttc acataacccg gcagcaacat ccaggaagac ctgtctgatg
      241 ctccttctgg ctgctgcctc ataaaactcc agcgcggcac cttcaacacg gtccagcgag
      301 atgtccaggt caaaaatttc accgtcaaag cgttttttgt cccgtaacgc taaagttacc
      361 gtaactttat tctcaaaatt gcggatccct ttcacaatca gttcatagtt ttgagtcatt
      421 gaattactct ccccgtgcag ccttacgacg gtcctctctg attttgaaat acaggttagt
      481 cagatatgtc agcagcccaa acagcagact ccccagcacg cctattgccg cccactgaga
      541 cggggaaacc ctgtccagca actgcaggaa ccagtagccc gttcccaccg ctgacgtggt
      601 gtatgacaca cctgttgtga ttttttccat ctggtacata ccccgtctcc cgttatccgg
      661 aagctgacaa caataaaaaa agccaccagt taagtactga tggctctgat aactcatgca
      721 ggcatctcag acgacccact gacactaccg gtgagtttaa cgataccttc catttggctg
      781 gctcactttt tatgatgatg ccggtgcatt tatctccagc accagacttt ctatctcaac
      841 gccatacgct gcatttttgg taatatccgt cagcgtcagc gcattcagcc ccagtgtcag
      901 actgtctttt atgacctgga atgccgggcc agccactcca ttcagtttcg gagtaaccgt
      961 ggcactgccg gcggtgaaca ccagctccag cgtcagccag tcgttactgt aattcccgaa
     1021 ctcgcccaac tttgtgtttc ctgctttctt gtgatgcatc agattcagtt tgccgtctgt
     1081 ggtctgggtg aagaacgaca tcaggaacgg gttaccagtc ccggtcatcg ccacgacgtc
     1141 aggtaacgct acatcggtat acagataaat tcccagaccg aactggttgt tggtcagtgc
     1201 gcctgacagt cgaaacttac agctcagtct gccaccccgt gtcagcaggg agactgcgtc
     1261 atccaccggg cgcgtcaggg accaggcttt attgctctgc ttggtgatct taaatacacc
     1321 atctgacaac tgaactccgc cattcttaat gctccagccc tgcgcagcag cgtctccggc
     1381 tgtcggcaac agggagattg tgcgtatgga tgcatcttca gacggccccg atggcgtgtc
     1441 gccgccgggc gagggtttga tttccggtgc cttaccacta atgaaggcta aggtgcgacc
     1501 ggctacgttc agaatagcag ttgccatacg atcgggaata atgccacgac gcgcccatga
     1561 gctgaaatgc gtcgggcgat ttgatgatac ccagtttttg ttcgttcggg atgccgaacc
     1621 gtaataacca gacccggcaa tatcaggatc ttctgacggg ttgtttgtcg gtgtattaac
     1681 tccgctacca tcggtcataa agggaacaaa ataaatctgc tgggattctt tacctttata
     1741 tgcaccatat accacttcat attgcgtacc gtgttcttgt ttccacgcgt atgtcgtgtc
     1801 gccacaaatc caggggactg atgccggact tccaccgtga cactgcgccg ccagcccggc
     1861 aaggtcagca cggaactgct gtaccattgc aagaaatgct gctggctgct gggcgtaact
     1921 ggcattcgtc atatcgaatt ccccctgcat ccagcatatc gccagcaaaa cgtttttcgg
     1981 gtttttctgc aatgctgcct tcgtgcggaa aagcagatcc tgatataacg gcttacccac
     2041 tccccagcga gccgaatcct gactggcccc cgtggactcg ctgaatgtcc cctccgtgcc
     2101 ctgggtgaat gccgaaccac cacgacagca tggtaccagc aggatccccg cattattagg
     2161 gatatacgga agcagttttt tggcaatatg taagccctgt ccgacacagc cgtactgccc
     2221 tttgctcagg tcagcccggg gatggttaat cgtactcata tcctgaacat catgcagaca
     2281 atggtcagca ggaatgatgt cgttaaatac gcatacttca ccaccgggag tcactgtgtt
     2341 acgacgggcc agttgcttaa tgcgcggatg gggcgcatcg tatgaatccg gaagcggaag
     2401 accttcaccg taagccatgg cattggattg cccggccagt acgatgacgt agtaccactc
     2461 cggctcagtt gcaccactga cgaccacatc accttctgct gcaatcgcct gcatcagggt
     2521 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctccttca gtttttgtgt
     2581 cagtcgtttc gcaaggtctg acggcgatgc cgccctgacc acgtcatagt gtttaaatgc
     2641 catgaatcct cccggccggg ataatattgt gagtaaaata aggagcgggc tgaagtccgg
     2701 aagttacagg acaatggcag aagagagacg acagcccgca attcgaaaaa gaccgcgcag
     2761 ttgcgcagag tgattactat ggggtattat tcgccagctg aaatattact tcacgtttta
     2821 ttgtttattc ctt
//
LOCUS       DAJTXQ010000253         2816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000253 DAJTXQ010000000
VERSION     DAJTXQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.259"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..991
                     /locus_tag="OO515_004166"
                     /pseudo
     CDS             1..991
                     /locus_tag="OO515_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1007..1756
                     /locus_tag="OO515_004167"
     CDS             1007..1756
                     /locus_tag="OO515_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6577933.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1758..>2816
                     /locus_tag="OO515_004168"
     CDS             1758..>2816
                     /locus_tag="OO515_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577934.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          886 a          566 c          585 g          779 t
ORIGIN      
        1 taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc agcagtaatg
       61 tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa aacgtctccg
      121 cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa ctgtacctaa
      181 atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa cgaaatcagg
      241 ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat aattcctgga
      301 gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca
      361 gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca cagcgagaca
      421 gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt cacggagccg
      481 ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca gatggtgcag
      541 aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc ggagttattc
      601 catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat cttccagaaa
      661 atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct attggctacg
      721 ccaaacttga taccccaagg gctatcaaat catgggggtt attcgcctgg cagatggtag
      781 tcacccacca ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt
      841 tgctgatggc ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg
      901 ctggggtgat aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac
      961 ggttatttta ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa
     1021 cacaaagaat ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg
     1081 tgccatcatc ccggaccgta cccgtatcat aatgaatgag tcagataaag gggaagcatt
     1141 aaaacttact aatcaaagca aaaatctacc ctatctggcg caaacatgga tagaagatac
     1201 gaaaggcaat aaatcacgtg actttattgt cacggttccc cctatggtac gtttaaatcc
     1261 cagtgagcaa attcaaataa gaatgataag tcaggaaaaa attgctcaac tgcctaatga
     1321 cagagaaacg ctcttctatt ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa
     1381 tgtgatgcaa gtcactatgc agcacgcatt gaaactattc tggcgaccaa aagctattga
     1441 attagaagat gatggggtaa tgtcttacga aaaagttgaa atcatccgta gaaatgatgg
     1501 ttcgatccgc tttaacaata agatgcctta tcacgttacg ctgggttata tcggaacaaa
     1561 cggaatcaca atgttgccac aaacgcaaag tttaatggta accccttata gccatgcaga
     1621 tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt tatatcaatg attttggtgg
     1681 attaagtttt tatgaaataa actgcccgac ggttaataac tcttgtaatg tttccgtagc
     1741 caaacgagat aaataatatg cgctacttat taattgtaat tatatttatt ataggtttta
     1801 gtgcattacc tgtatgggca atggactgct atgctgaaca tgaaggggga aataccgtag
     1861 tcattggtta cgtaccaaga atttctatcc ccagtaatgg taaaaaaggt gataagatct
     1921 ggcaaagcag tgagtatttt atgaatgtct tctgtaataa tgcattacct gccccatcac
     1981 ctggagaaga atacccatca gcctggacta atataatgat gtttttacca ggcggtcagg
     2041 acttttataa tcagaactcc tatatttttg gcgtaacata caatggagtt gattatgatt
     2101 cgactgcccc taatgctatc gcagcaccag aatgcattga tataaaagga gcgggtacct
     2161 ttaataatca ctataaaaac cccgctgtct gtagtggagg tcctgagcct caattatcag
     2221 taacctttcc agctcgcgta caactctata ttaaattagc aaaaaatgcc aatagagtta
     2281 ataaaaacct ggtgttacct gatgaatata tcgctctaga gtttaaaggt atgagtggcg
     2341 caggagctat agaagtagac aaaaatttga ccttcaggat tcgtggatta aataatattc
     2401 atgtccttga ctgctttgtt aatgtcgcac tggaaccagc tgatggcgtt gttgactttg
     2461 gtaaaataaa ttcccgaaca attaaaaata ccagcgttag tgagacgttt agtgtagtca
     2521 tgacaaaaga tcctggtgcg gcctgtactg agcaattcaa tattttaggg agctttttca
     2581 ctacggatat tttgagtgat tatagccatc tggatatggg taatggactg ctattgaaga
     2641 tatttcataa agatggaaca gcaacggaat ttaaccgctt ctcacaattt gcttcttttt
     2701 catcgtctag tgcaccttcg gtcaccgcac cattcagggc agaacttagt gcgaacccgg
     2761 cagagactgt tgttgaggga ccgtttagta aagacgtaat cctcaaaatc acctat
//
LOCUS       DAJTXQ010000254         2796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000254 DAJTXQ010000000
VERSION     DAJTXQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.260"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..616
                     /locus_tag="OO515_004169"
                     /pseudo
     CDS             1..616
                     /locus_tag="OO515_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            613..1092
                     /locus_tag="OO515_004170"
     CDS             613..1092
                     /locus_tag="OO515_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR6577935.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1146..2096)
                     /gene="corA"
                     /locus_tag="OO515_004171"
     CDS             complement(1146..2096)
                     /gene="corA"
                     /locus_tag="OO515_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6577936.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2466..>2796
                     /locus_tag="OO515_004172"
     CDS             2466..>2796
                     /locus_tag="OO515_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577937.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          620 c          590 g          743 t
ORIGIN      
        1 ccggaacaaa aatatcacaa cgtgaatgca ctgatatttt atttgcagaa atgaaagaac
       61 tagcaaaaat gttttccttt tttggacaat ataaaacact tatcgaagat ctgattgaac
      121 attttagata tggaaatggc agcaattttc atagccaaca attaaattta tcttttcatg
      181 aaaaaataaa tatggttata atagtccaat acgaataatc aaagaatgta tcgaaaatgg
      241 cattaattca actccctcga caggatacca acctcttatc ctgcaaagca ttaaaaccaa
      301 attactttca tcaagactaa ataaatttaa tgacttcgag gacagtttta atggcttagg
      361 aatcagtgtt catgatattt cagctcaaaa aatatctctt ttaagttttc aaaaatacgc
      421 tataggatgg agtgcaacaa ttcattttgt agcacaggac cacttcggtc ttgatgtaac
      481 tgatatcaaa aacaagactt acagcaaata tagatttttt cgcatatggt tctttttaca
      541 acggcataaa gactttgctt tcaaaccttt tttcaccaat tttaacacta tcgaaagaat
      601 cgaaaattat ctatgaaaag caaactccag aaaattatat tatgtttatt tcttctatgc
      661 tgcatctata atttatggac attgcgtcct gtacagattt tgtatacata ctctgatgct
      721 ggcaattcag tctttctggt cgttgatcat ctgccatgga ctgacagcga taaaataaat
      781 tggtatctga agcatcagaa tgaaattaaa aatcaacatc ccctaccaga aggtagttgg
      841 catacctggt atgtcattga tatcggtaat ggcttcactg actataaaaa atatatcgaa
      901 gggccgtatg aagatctcta ctgtttcccg acaattaaga gcaatgataa ttgcatcgtg
      961 aaaaattact taatggttat taatgaatac ccttatcgta acactcatat tggtattaac
     1021 gattttactg agtatcaatt aacacaagaa aataaaatag agcgagtttt caatccgcat
     1081 gactttaaat aagacaattt ttagaaataa gccggataaa aaaatagctt tatccggcac
     1141 tcatattaca gccagttctt gcgcttaaag tacagatacg gtgccaggcc cgcgaggatc
     1201 ataaagataa tcgcgcctgg gtagccgaaa ctccacttca gttctggcat aaactcaaag
     1261 ttcatcccat agctggaagc aacgagcgtc ggcggcagga ataccacgga taccaccgag
     1321 aagattttga tgatgcggtt ctgctcgatg ttgataaaac ccatcgccgc ctgcatcagg
     1381 aagttcacct tctggaacag ggattcgtta tgcggcagca gggattcgat atctcgcagg
     1441 atttcacgcg cctgctccag ttgcccaccc ggtaaacgcg ctttacgcac caggaagttg
     1501 agcgcgcgct gggtatccat cagacacagg cgaactttcc agccgatatc ttccagttcc
     1561 gccagagtgg agagcgcctc gtcgtactca tcgccctgat gcccttccat aatcacccgg
     1621 ctcaactgct ccaggtcgct atagatattt tcaatttcat ctgccaactg ttcgattttg
     1681 gtttcgaaca gatccagcag cagctcgtag gcgttaccgt ctaccatcga ctggctacgg
     1741 gcacgcatac gatacagacg aaaagcgggc agttcacgct cacgcagagt aaacagacga
     1801 ccatcacgga tggtaaatgc cacagtggag ttaccggcgt gatcttccgc atcttcaaag
     1861 aagaagaagg agtgaatatg caggccgtcg tcgtcttcaa agaaacgtgc cgatgcttcg
     1921 atgtcttcca gttccgggcg ggttgccagg ctctggccaa gttcagattg tacgcgcagt
     1981 cgctcgtcgt cgtccggttc gacaagatca atccatactg catttacaag gggttgtgac
     2041 tcttcgactt ccagccgggt cagtcggtta ttttccagtt gaaatgcgct cagcatgacc
     2101 gggactccca atgcttaaaa tatcggacag ttcggtgggc acacagaaac aaattgggtt
     2161 tcagaccatt aaacagcctg actcagcgcg acgggaaaaa ttgaggtcgc tgacaaccgc
     2221 taaggctatc agcaaaaagg gatagcctta ggagttgatc ctggatgaca ggatagtgag
     2281 ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta gagtgatcgt gcgcgcatgt
     2341 tacgccagca aaattttgcc gtcaacacgc aacagaacac cacagaacaa tagttgaact
     2401 tcgcgctttt tggtactcgt caaggtcgct aacaggataa aaaaatccaa catccaggac
     2461 ctataatggc gcatcggcta ctcattggta aaggaatgat caccttgaat ctcaaacgga
     2521 tttttctcgc cctcacctta cttcccttat ttgctgttgc cgctgatgat tgcgcactct
     2581 cagatccgat gctgaccgta caggcgtata ccgttaatcc tcaaacagag cgggtgaaaa
     2641 tgtactggca aaaagccaat ggcgaagcgt ggggaacttt acatgctctg ctggtggata
     2701 tgaatagtca gggtcaggtg cagatggcga tgaacggcgg catctatgat gaaagctatg
     2761 cgccgctcgg tttgtacatc gaaaacggtc agcaga
//
LOCUS       DAJTXQ010000255         2796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000255 DAJTXQ010000000
VERSION     DAJTXQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.261"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..199)
                     /locus_tag="OO515_004173"
                     /pseudo
     CDS             complement(101..199)
                     /locus_tag="OO515_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(245..427)
                     /locus_tag="OO515_004174"
     CDS             complement(245..427)
                     /locus_tag="OO515_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6577938.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            526..1416
                     /locus_tag="OO515_004175"
                     /pseudo
     CDS             526..1416
                     /locus_tag="OO515_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1419..1952
                     /locus_tag="OO515_004176"
     CDS             1419..1952
                     /locus_tag="OO515_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6577939.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1981..2508)
                     /locus_tag="OO515_004177"
     CDS             complement(1981..2508)
                     /locus_tag="OO515_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6577940.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2510..>2796)
                     /locus_tag="OO515_004178"
     CDS             complement(2510..>2796)
                     /locus_tag="OO515_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6577941.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          778 a          626 c          615 g          777 t
ORIGIN      
        1 ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc
       61 acgttcttgc ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg
      121 taggccactc aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact
      181 ttttctaggc ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca
      241 gttttcagac aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag
      301 gtcggcctgt ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa
      361 caagagcgtt tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa
      421 tcagcacgct gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc
      481 attatctaaa acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc
      541 agtacagcga atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta
      601 tccggcaatg cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct
      661 tttgatggtt cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg
      721 aaaacaggaa gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc
      781 tataacgcaa ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga
      841 agttgtcctg cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca
      901 gcccgtgatg gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct
      961 tcagcaggag atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt
     1021 atcggaacat ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc
     1081 tttaaacaaa aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc
     1141 tttgtctccg gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc
     1201 tcggattacg gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt
     1261 gtttagacca tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt
     1321 ggtattatcg gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta
     1381 attaacggaa catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg
     1441 tcgcaggtaa tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct
     1501 ggttatggtc cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata
     1561 caataaaact ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag
     1621 caattaatcc agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg
     1681 ctgatatttc gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg
     1741 aggaagagca aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg
     1801 ataaaaccca gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac
     1861 aaaaattaac cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc
     1921 tgccagtaac gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt
     1981 ttattcaggc ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact
     2041 gtagcgttcc catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac
     2101 agctcgttcc agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga
     2161 ttcggcctgt tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag
     2221 aaacatccat tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac
     2281 tatgcttagc ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt
     2341 gttctcatcg taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca
     2401 gtttttgcca tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg
     2461 ttccggtgtt ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc
     2521 ccacgttgta ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga
     2581 caatatcatc cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa
     2641 agccgtagtc gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga
     2701 catgattatg cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc
     2761 cactagacgc aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAJTXQ010000256         2732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000256 DAJTXQ010000000
VERSION     DAJTXQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.262"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="OO515_004179"
     CDS             complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="OO515_004179"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6577942.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLIRAQIAITAA"
     gene            complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="OO515_004180"
     CDS             complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="OO515_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6577943.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1891..>2732)
                     /gene="bcsE"
                     /locus_tag="OO515_004181"
     CDS             complement(1891..>2732)
                     /gene="bcsE"
                     /locus_tag="OO515_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6577944.1"
                     /translation="WQLFNNNEVLFNEARTAQAATVVFSLQQNAQIEPLARSIHTLRR
                     QRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTMIESVQGQKFSRY
                     VPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGVLVALRPVPGIRV
                     EQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLPTGDIFSNRMVWF
                     EDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRIPEPMRLLDDAVE
                     RSS"
BASE COUNT          692 a          696 c          728 g          616 t
ORIGIN      
        1 agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc
       61 aggcgaacgt acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc
      121 ggtgctgttt gtggcaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa
      181 atcttgccat caagaacgcg aaccaccaga tcggagatag ctaggaagct gctcggttgg
      241 tcaatgacaa tcggtgcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg
      301 gggacgtctg tgatagacgg gctagggata tcacgtaggc cagatacctg cattctgtcg
      361 cccttcagcg cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc
      421 tccagttcag taaagaaggc gtccagttca tcaaagaatt tctgcgcccg cgctttgtaa
      481 tccgctgttt tgctgacccc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag
      541 aacgtggcac tacggctgtt tttatctttt tcggtaacgt ccagccagcg gttaagcacg
      601 gcggtatcgt cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt
      661 tgatccatca attcagtctg catgccgcca ttttcgcgaa cttctttcaa aaaaccgccg
      721 aactggccgt tatgtcccat catcaggtgc tgggtaaagc ccagtttcga aaggttatca
      781 aacagatagc agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag
      841 ctggcgcgca gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc
      901 ttgaactcaa tatcgaaatg cgaccacagt ggatgcgaca tcaacccggc ggcttctata
      961 tccgaccagg aaagcgaaca gatgttaatc accagtagtt caaatggctg agcatcagcg
     1021 ggcagcgaag acgggaaggt cgatttacgt ttcgcctccg cgttatagaa attattcagc
     1081 caggcgttaa ggttcgccgt tgttggcggt gcagtttgtg cgggcatatc acccactacc
     1141 gatgcgccac ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg
     1201 gtcgtcggtt gtccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc
     1261 agccatagca gtatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac
     1321 caggccacta ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca
     1381 aggtcgatta aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt
     1441 tccgggccag gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc
     1501 cagtggcgca agcgatgcag gctgtagcgc ggaatgggca tcagcagaaa cgtggcaaac
     1561 accaaattga ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc
     1621 agaaaataga agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg
     1681 gcggtatttt gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta
     1741 acgtcccggc cggtttaaca taacgaggtt tagcaaagaa taaacgtaag gtgtcgcgaa
     1801 tgcgtcgcaa aaagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga
     1861 caataatttc aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa
     1921 cagtcgcatg ggttctggta ttcgtcgcca gtggcgacca tcgtgctcgg cattgatgac
     1981 cggtttagaa ctttgcgtta aaggcagcgg catgccccat tgttctgggg caagcaagcg
     2041 catctgcacc agctcggcac tgatttgatc atcttcaaac cagaccatac ggtttgagaa
     2101 aatgtcgcca gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg
     2161 acagaatgag agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg
     2221 gttagggcga cacagcgtca gggcttgttc aacgcggata cccggtaccg gacgtagggc
     2281 aaccagaacg cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc
     2341 attacagaac acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag
     2401 caaggtagtg atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat
     2461 catcgtcaga caacgggaga gtggcgcatt ccacggaata accatatttg caccgcaggc
     2521 caataacaaa cgttcatcgg tggcgcgcag gctagcggta ttttcccgca cgaggatttt
     2581 catcgcacta ccgcgctggc gacgcagggt atgaatgctg cgggccagtg gctcgatttg
     2641 cgcattttgc tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa
     2701 caggacttcg ttattgttga acagttgcca gt
//
LOCUS       DAJTXQ010000257         2726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000257 DAJTXQ010000000
VERSION     DAJTXQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.263"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(423..680)
                     /locus_tag="OO515_004182"
     CDS             complement(423..680)
                     /locus_tag="OO515_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577945.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OO515_004183"
     CDS             complement(677..1174)
                     /locus_tag="OO515_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6577946.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="OO515_004184"
     CDS             complement(1376..1834)
                     /locus_tag="OO515_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6577947.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="OO515_004185"
     CDS             complement(1831..2328)
                     /locus_tag="OO515_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6577948.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="OO515_004186"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="OO515_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6577949.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          707 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataac
//
LOCUS       DAJTXQ010000258         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000258 DAJTXQ010000000
VERSION     DAJTXQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.264"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..769
                     /locus_tag="OO515_004187"
     CDS             230..769
                     /locus_tag="OO515_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6577950.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO515_004188"
     CDS             complement(995..1318)
                     /locus_tag="OO515_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6577951.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO515_004189"
     CDS             1604..1798
                     /locus_tag="OO515_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6577952.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTXQ010000259         2689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000259 DAJTXQ010000000
VERSION     DAJTXQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.265"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1386
                     /gene="fimD"
                     /locus_tag="OO515_004190"
     CDS             <1..1386
                     /gene="fimD"
                     /locus_tag="OO515_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
                     /protein_id="HCR6577953.1"
                     /translation="TAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYA
                     KSMYTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYHNL
                     RFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSLSF
                     SWNESVGIPDNEHIVGLNVSVPFNVLTKRRYTRENALDRAYASLNANRNSNGQNSWLA
                     GVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNWQL
                     SGGVVGHENGITLSQPLGDTNVLIKALGAGGVRIENQTGILTDWRGYAVMPYATVYRY
                     NRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGS
                     LVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVT
                     VISAVCTHPRS"
     gene            1399..2406
                     /gene="fimH"
                     /locus_tag="OO515_004191"
     CDS             1399..2406
                     /gene="fimH"
                     /locus_tag="OO515_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCR6577954.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2417..>2689
                     /gene="sfmF"
                     /locus_tag="OO515_004192"
     CDS             2417..>2689
                     /gene="sfmF"
                     /locus_tag="OO515_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCR6577955.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTG"
BASE COUNT          664 a          592 c          702 g          731 t
ORIGIN      
        1 accgcctttt tattagggct ggggcgcaac ctcgggaact ggggcgcagt gtcgctggat
       61 gtgacccatg cgcgcagcca gttagccgac gacagtcgtc atgaggggga ttccattcgc
      121 ttcctctatg cgaaatcgat gtacaccttc ggcaccaatt ttcagttaat gggttaccgc
      181 tattcgacac aaggttttta tacccttgat gatgttgcgt atcgtcgaat ggaggggtac
      241 gaatatgatt acgattacga cggtgagcat cgcgatgaac cgataatcgt gaattaccac
      301 aatttacgct ttagccgcaa agaccgtttg cagttaaata tttcacaatc acttaatgac
      361 tttggctcgc tttatatctc tggtacccat caaaaatact ggaatacttc ggattcagat
      421 acctggtatc aggtggggta taccagcagc tgggttggca tcagttattc actctcattt
      481 tcgtggaatg aatctgtagg gatccccgat aacgaacata ttgtcggact taatgtttca
      541 gtgcctttca atgttttgac caaacgtcgc tacacccggg aaaatgcgct cgaccgcgct
      601 tatgcctcct tgaacgctaa ccgtaacagc aacgggcaaa atagctggct ggcaggcgtt
      661 ggtgggacct tactggaagg ccacaacctg agttatcacg taagccaggg cgatacctcg
      721 aataatgggt acacgggcag cgccacggca aactggcagg ccacttacgg tacgctgggg
      781 gtcgggtata actacgaccg cgatcaacat gacgttaact ggcagctgtc tggcggtgtg
      841 gtcgggcatg aaaatggcat aacactgagc cagcctttag gggataccaa tgttttgatt
      901 aaagcgcttg gcgcaggcgg tgtacgcatt gaaaatcaaa ctggcatttt aaccgactgg
      961 cgcggctatg cggtgatgcc gtatgccacg gtttatcggt ataaccgtat cgcgcttgat
     1021 accaatacga tggggaattc catcgatgtt gaaaaaaata ttagcagcgt tgtgccgacg
     1081 caaggcgcgt tggttcgtgc caattttgat acccgcatag gcgtgcgggc gctcattacc
     1141 gttacccagg gcggaaaacc ggtgccgttt ggatcactgg tacgggaaaa cagtaccgga
     1201 ataaccagta tggtgggtga tgacgggcaa gtttatttaa gtggtgcgcc attgtctggt
     1261 gaattactgg ttcagtgggg agacggcgcg aactcacgct gcattgcgca ctatgtattg
     1321 ccaaagcaaa gcttacagca agccgtcact gttatttcgg cagtttgcac acatcctcgc
     1381 tcataaagga aattatcaat gaagataatc tgcagattat tattggcaat ggcatgtttg
     1441 tgtctggcaa acatatcctg ggctactgtt tgtgcaaata gtactggcgt agcagaagat
     1501 gaacactatg atctctcaaa tgtctttaat agcaccaata accagccagg gcagattgtt
     1561 gttttaccgg aaaaatccgg ctgggtaggt gtctcagcaa tttgtccact cggcacgctg
     1621 gtgaattata cataccgtag ttatgtcact aactttattg ttcaggaaac tatcgataat
     1681 tataaatata tgcaattaaa tgattatcta ttaggtgcga tgagtctggt tgatagtgtg
     1741 atggatattc agttcccccc gcaaaattat attcggatgg gaacagatcc taacgtttcg
     1801 caaaaccttc cgttcggggt gatggattct cgtttgatat ttcgtttaaa ggttattcgt
     1861 ccctttatta acatggtgga gatccccaga caggtgatgt ttaccgtgta tgtgacatca
     1921 acgccttacg atccgttggt tacacctgtt tataccatta gttttggtgg ccgggttgaa
     1981 gtgccgcaaa actgcgaatt aaatgccggg cagattgttg aatttgattt tggtgatatc
     2041 ggcgcatcgt tatttagtgc ggcagggccg gggaatcgac cagctggcgt catgccgcaa
     2101 accaagagca ttgcgatcaa atgtacgaat gttgctgcgc aggcttattt aacaatgcgt
     2161 ctggaagcca gtgccgtttc tggtcaggcg atggtgtcgg acaatcagga tttaggtttt
     2221 attgtcgccg atcagaacga tacgccgatc acgcctaacg atctcaatag cgttattcct
     2281 ttccgtctgg atgcagctgc ggcagccaat gtcacacttc gcgcctggcc tatcagtatt
     2341 accggtcaaa aaccgaccga agggccgttt agcgcgctgg ggtatttacg cgtcgattat
     2401 caatgaggta cggagaatga gaagagtact ctttagctgt ttctgcgggt tactgtggag
     2461 ttccagtgga tgggcagctg accctttggg aacgattaat atcaatttgc acggtaacgt
     2521 tgttgatttc tcctgtaccg taaacactgc ggatattgat aagacggtag atttaggcag
     2581 atggcctacg acacaattac tgaacgctgg cgataccacg gcactcgtcc cttttagcct
     2641 gcggctggag ggatgtcctc cgggttccgt tgcgatttta tttacggga
//
LOCUS       DAJTXQ010000260         2665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000260 DAJTXQ010000000
VERSION     DAJTXQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.266"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(2..418)
                     /gene="rraB"
                     /locus_tag="OO515_004193"
     CDS             complement(2..418)
                     /gene="rraB"
                     /locus_tag="OO515_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6577956.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            580..1584
                     /gene="argF"
                     /locus_tag="OO515_004194"
     CDS             580..1584
                     /gene="argF"
                     /locus_tag="OO515_004194"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6577957.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(1656..>2665)
                     /locus_tag="OO515_004195"
     CDS             complement(1656..>2665)
                     /locus_tag="OO515_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6577958.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          759 a          594 c          521 g          791 t
ORIGIN      
        1 attagtggcg aaccccgtca tcgtcttcat cgacaaaatc ttcatcgtcg ccatcttcgc
       61 cgttaggatc ttcaaagtaa gtgccccaac cgtcgtactc aacgtcaaat ttctccgcca
      121 gcgtcatcag ttgttcaacc tgggcatcga tcagatcggc attcaacgcg cactcgctga
      181 ggatgtcgca gcaaatcacg atatcaccat cttcaacttc cagctcttct ggatcggtca
      241 cttcgtaacc gagtttaaac gcttcaactg ccgctttttc cagagtttcc agatcgtctg
      301 cggaaagatg gtgttcgatg gtgtacaacg cgtccggatc gctgccatct tccagtaatt
      361 cttcaataat caaacgtgtt tcttcacgct gttcttccag ttgttccggg tttgccatgg
      421 ctcattcctc ttaatgtcct gccgatactc ttattgtcac acaccactga cattgcctcc
      481 acctttgtga taaagattta tgctttagac ttgcaaatga ataatcatcc atataaattg
      541 aattttaatt cattaaggcg ttagccacag gagggaccta tgtccgggtt ttatcataag
      601 catttcctga aattactcga tttcacgcca gctgaactca acagcctgct gcagttagcc
      661 gcgaagctga aagccgataa gaaaagcggt aaagaagaag ccaaactcac tggtaaaaac
      721 atcgcgctca tcttcgaaaa agactcgacc cgtacccgat gctctttcga agttgccgca
      781 tatgaccagg gggctcgtgt tacttatctc ggcccaagcg gcagccaaat tggtcataaa
      841 gagtcgatta aagacactgc ccgcgtgctt ggtcgcatgt atgacggtat tcagtatcgc
      901 ggctatggtc aggagattgt cgaaacactg gcggaatacg ctggcgtgcc ggtatggaac
      961 ggcctgacca atgagtttca ccccacgcag ctgctggcgg atctcctcac catgcaggag
     1021 catttgcccg gcaaaacgtt caacgaaatg acgctagtct atgcaggtga cgcacgtaac
     1081 aacatgggca attcgatgct cgaagctgca gcgcttaccg gtctggattt gcgtctggtc
     1141 gcgccacagg catgctggcc ggaagctgcg ctggttacgg aatgccgcgc cctggcacaa
     1201 caaaatggcg ggaatattac gctgacggaa gatgtagcta aaggagttga aggtgctgac
     1261 tttatctata ccgatgtatg ggtgtcgatg ggtgaagcaa aagagaaatg ggctgaacgc
     1321 attgcattgc tgcgtgatta tcaagtgaac agcaagatga tgcagttgac cggtaacccg
     1381 gaggtcaaat tcctccactg cctgcccgcg tttcatgacg accaaacgac gcttggcaaa
     1441 aaaatggcgg aagagtttgg cctacatggc ggaatggaag tgactgatga ggtcttcgaa
     1501 tctgccgcca gcattgtatt tgatcaggcg gaaaaccgca tgcataccat caaagcggtg
     1561 atggtcgcga cgctcagtaa ataattgata tcgctataaa gatgccggga aatacaaaaa
     1621 catcccggcg tcacattatt ttatgcagtg atagttcaat tattccaccc caattttttt
     1681 attgctatac gtaaatcttc cggaggattt aattttccta actgttcttc attaatatat
     1741 ccatatatat tgattttgcc attccggagg attttcatca ttcttgttct aatttttaat
     1801 tcattaccca tcttttcttc aaaaatctta acgacataac ccggaagttt atttacaatt
     1861 tcttttgttt tttcttcatc accataaaca tatcctaact cattaagata cagctgttcg
     1921 ccctcataac acttaagtaa ttgatagaag taccacttag caaaatcagg ttgcttcatg
     1981 aaaaaatcct tcaaaaaaat atcatttact aaaatgctga caggcacttc attaaatagt
     2041 aacttaacat catcaaataa ccttagtatt aagcgtgatt tttcttgatt gcttaagcta
     2101 aaatcattga catatgcaac caacttatcc caaatcaatt cctgtaattt acggctattt
     2161 ttatagggtt ctttatcgag attgaaaaat aaggtctcag ctattttaat tttagattga
     2221 tcgcagctaa aatctttaga ttgatcgcag ctaaaatcaa tatgatttat cacggagcat
     2281 gcatacttta tcttttcaga atcaccaaaa tattgcattc ttttattaaa acaccaatcc
     2341 agtattctta catcattaat atatggggat tttaaaaaag tctccagaat cgaatcgatg
     2401 accggtgtaa ggttaacttt tgcatcatct aaggaatcaa taagaatatg agacaggtta
     2461 actttgatgt cattaatttc attgtcagta gctttcttat tattaagaag ataattattt
     2521 actgcgttaa ggtcatctat ttctttcagt atcccttttt caccaagcaa gtatgaaatc
     2581 gattcattac atacataagg cattaatagc agatgttctt tccttattgc taataatgca
     2641 ttgttcaatg gtttttctaa taaat
//
LOCUS       DAJTXQ010000261         2563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000261 DAJTXQ010000000
VERSION     DAJTXQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.267"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2412
                     /locus_tag="OO515_004196"
                     /pseudo
     CDS             1..2412
                     /locus_tag="OO515_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            2414..2563
                     /locus_tag="OO515_004197"
                     /pseudo
     CDS             2414..2563
                     /locus_tag="OO515_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          697 a          533 c          727 g          606 t
ORIGIN      
        1 attttaggat atcaagcgaa cagtaatggt aaggttatcg ttgaaaaggg tggcgagtgg
       61 ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg agctggggag
      121 gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc
      181 accggtatcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc
      241 tataatgggt atttcggtaa tggcacagtc aatatttcca ataatggtct gattaataac
      301 aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat
      361 aaagggcatt ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat
      421 gctggtgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact
      481 gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc
      541 actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag
      601 gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat
      661 gtcagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt
      721 gttgcgggcg tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt
      781 acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca
      841 tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt
      901 aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaag
      961 gggatagtta atatttcaac ggacagtctc tggaatttaa agacatcatc tactaacgca
     1021 caattgctac aggtcggtgt attaggcacg ggtgaactga atattaccac cggaggtata
     1081 gttaaagcgc gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg
     1141 gatgggcaga actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt
     1201 acgttaaccc tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt
     1261 gtttttgaac ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca
     1321 gatgccgggt atataaccaa cgcgacgaaa gtagagtttg gttctggcga aggtgttttt
     1381 gtctttaatc atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt
     1441 gacgataaag acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat
     1501 acttatagcg gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca
     1561 gatggggtaa cgggcatggg ttcgagtgaa gtcaccattg caagccccgg tacgctcgac
     1621 attctcgcat caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat
     1681 ggcttgatgc gagtgcagct gtcatcctac gacaagatgt ttggctttac ccatgcaaca
     1741 gggactgaat tcgccggtgt tgcccaactg aaagacagaa ccttcactct ggaacgcgac
     1801 aacaccgctg cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta
     1861 aacgtgggag agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat
     1921 acggatattc ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc
     1981 ggcgcgggtg actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg
     2041 cttatcgacg tgcctaaacc gtggaatgat cccatagcga ataaccctct gacgacgctc
     2101 aatttgctgg aacacgatga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt
     2161 attgggtcgg gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac
     2221 aaaacgttac acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc
     2281 ctcacgaccg caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc
     2341 catggtgggc aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat
     2401 atgtcggcaa aatagccatc gcttacacga ccgtctgggt gagccgcagt atacagattc
     2461 actgcattct cagggatcgg caagcagtat gtggatgcgt catgtcgggg ggcacgaacg
     2521 tttcaggacc ggtgacggcc agctaaatac tcaggctaac cgc
//
LOCUS       DAJTXQ010000262         2556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000262 DAJTXQ010000000
VERSION     DAJTXQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.268"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OO515_004198"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OO515_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6577959.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="OO515_004199"
     CDS             complement(390..1178)
                     /locus_tag="OO515_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6577960.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="OO515_004200"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="OO515_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6577961.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OO515_004201"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OO515_004201"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6577962.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="OO515_004202"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="OO515_004202"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6577963.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAJTXQ010000263         2550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000263 DAJTXQ010000000
VERSION     DAJTXQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.269"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..98)
                     /locus_tag="OO515_004203"
     tRNA            complement(24..98)
                     /locus_tag="OO515_004203"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Gln,seq:ttg)
     gene            complement(122..206)
                     /locus_tag="OO515_004204"
     tRNA            complement(122..206)
                     /locus_tag="OO515_004204"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Leu,seq:tag)
     gene            complement(215..291)
                     /locus_tag="OO515_004205"
     tRNA            complement(215..291)
                     /locus_tag="OO515_004205"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Met,seq:cat)
     gene            complement(671..2335)
                     /gene="asnB"
                     /locus_tag="OO515_004206"
     CDS             complement(671..2335)
                     /gene="asnB"
                     /locus_tag="OO515_004206"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6577964.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT          616 a          642 c          632 g          660 t
ORIGIN      
        1 gtgaaccgct tactcgaaga agatggctgg ggtacctgga ttcgaaccag ggaatgccgg
       61 tatcaaaaac cggtgcctta ccgcttggcg ataccccatc cgtacaacgc tttctggtga
      121 atggtgcggg aggcgagact tgaactcgca caccttgcgg cgccagaacc taaatctggt
      181 gcgtctacca atttcgccac tcccgcaaaa aagatggtgg ctacgacggg attcgaacct
      241 gtgaccccat cattatgagt gatgtgctct aaccaactga gctacgtagc catctttttt
      301 tcgcgatacc ttatcggcgt tgcggggcgc attatgcgta tagagccttg cagcgtcaac
      361 ctctttttca aggaaaattg ctcgaaagtg actgtttggt taggttgcga acagcgtggc
      421 gctatattcg tcaattattg tttactttgt gttttttccc caccctacag ccattctttt
      481 gtcatacagg atgaaattcg gaattcaaca atggtggtgg gaaattaatc tatgaaatac
      541 tggcctacag tgatgagttg tcaaacagtg atgtggcaaa cccggaacat ttccttactg
      601 catatcagaa tcaacaagca cctcaataac tgaaacagcc ccggatttca ccggggctgt
      661 ttcgcatttc ttacttatac gccgactggt gaacgccaac cgcacgacca gacggatcgt
      721 ccattttctt gaacgcctca tcccactcaa tcgctttagc ggaagaacac gcgacggacg
      781 ggccgcccgg cacgcactca gcggcgctcg gaagcgggaa cagttcttca aagatctccc
      841 ggtacagata cgcttctttt gaggttggcg tgttgtacgg gaagcggaag cgggcagttt
      901 ccagttgctg atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg atccaactgt
      961 aaccgacgcc atcggagaac tgctctttct gccgccaggc cacgcttgcg ggcaggtatg
     1021 actcaaaaca ttcacgcagg atgtgttttt ccattttgcc gttaccgcac attttatcct
     1081 gcgggttaat gcgcatcgcc acatcaagga attttttgtc gaggaacgga acgcgtgctt
     1141 ccacgcccca ggctgacatc gctttgttgg cgcgcgcgca gtcatacata tgcagggcca
     1201 gcagtttacg caccgtctct tcatgcagtt ctttagcgtt gggcgcttta tggaagtaaa
     1261 gataaccgcc aaacacttca tcagaacctt caccggacag caccatttta atgcccatcg
     1321 ccttgatctt acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg gtcacatcat
     1381 aagtttcgat gtggtaaatc acgtcgcgga tggcatccag accttcctgt acagtgaagt
     1441 gaatttcgtg atgcaccgtg cccagatggt ttgccacttc ctgggctgct ttaagatccg
     1501 gtgaacccgg cagacctaca gcaaaggagt gtaactgggg ccaccaggct tcagagcgtt
     1561 cctgatcttc cacgcgacgg gctgcgtatt tcttggtgat agcggaaata attgaggaat
     1621 ccagaccacc agaaagcagc acaccgtaag gcacatcaga catcagatgg cttttaacgg
     1681 aatcttccag agcctgacgc agctcgtttt tgtcggtcac gttatctttc actgcatcaa
     1741 agtcgaacca gtcgcgatga taataagaac ggatttcgcc gtcctggctc cacaaatagc
     1801 tccccgccgg gaactcttta atcgtgcggc aaactggcac cagcgatttc atttctgagg
     1861 ccacatacag ctgaccgtgt tcgtcatacc ccatatacag tgggatgatc cccagatggt
     1921 cgcgaccaat caggtaggca tctttttcgc tgtcgtacag ggcaaaggca aacatgccct
     1981 gcaagtcgtc gagaaattcc ggcccttttt cctgatacag cgcgaggatc acttcacagt
     2041 cagaccctgt ctggaactga taacgatcgc catattcagc gcgcaatgcc tggtggttgt
     2101 agatttcacc gtttaccgcc agtacgtggg ttttttgttg gttgtagaga ggttgcgccc
     2161 ccgcgttaac gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta tcgctggcat
     2221 aaataccgga ccagtccggg ccacgatgac gcatcaggcg tgacagctcg agtgctttct
     2281 tacgctgctc aactgcgtct gttttgatat cgaatacgcc aaaaattgaa cacataacct
     2341 tctccgttaa cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa atgccgcaaa
     2401 gcagcactgt gcgcaagcga tttggcggtg aaaaaataaa aaacgtaatg gtgattgtcg
     2461 attggtgaaa aagggtctgg tgtgaggatg tatttattga tgaatcgata atttttagcg
     2521 ggttttattg aatggttata ttttacgggg
//
LOCUS       DAJTXQ010000264         2532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000264 DAJTXQ010000000
VERSION     DAJTXQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.270"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(108..1526)
                     /gene="phrB"
                     /locus_tag="OO515_004207"
     CDS             complement(108..1526)
                     /gene="phrB"
                     /locus_tag="OO515_004207"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6577965.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1523..2032)
                     /locus_tag="OO515_004208"
     CDS             complement(1523..2032)
                     /locus_tag="OO515_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6577966.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          640 a          656 c          579 g          657 t
ORIGIN      
        1 tcaggcctac gtatttcctg caatttattg aatttgcaca aatttgtagg ctggataagg
       61 cgttcacgcc gcatccggca tctgggctcg attgcctgac gcgtctgtta tttccccttc
      121 cgcgccgcct catacgctgc caacgtttgt actctcgcct ctttgtgctc gactatcggt
      181 tgcggataat ccagcttcac acctgctttc tgcgcccact tccacggctc atgcaccgat
      241 ttccccggta cattgcgtag ttccggtagc cactggcgga taaactcacc ttcgagatca
      301 aatttctcgc cctgggttgt cgggttgaaa atacgaaaat acggcgctgc atcggttccg
      361 gttgaagcgg cccactgcca gccaccgtta ttggctgcca aatcaccatc aatcagctgc
      421 gacatgaaat atcgctcgcc ttcgcgccag tcgatcaata aatctttcac cagaaaactg
      481 gctgtaatca tccgtagcct gttatgcatc cagccagtgc tgttaagctg acgcatagcg
      541 gcatcaacaa tcgggtatcc cgttttgcct tcctgccagg cctgtaaatg tgcgggattg
      601 ctctgccact gtacacgatc cgtccaggca ataattggac gatgtttaca caacgagggg
      661 tgatacgtta tcaggtgacg gtaaaactcg cgccagatca gctcattaag ccagacacta
      721 ccgaccccac cgtccagcgc ctgcggctgt tcagccaaca agcgatgcaa gcactggcga
      781 ggcgataacc cgcccgttgc caggctggcc gacaaacggc tggtgccttc cactgccgga
      841 aaatctcgtt gttgctcata ttctccggca ccgttctggc aaaactggcg taattgcgca
      901 atcgccgctt tttcttccac cggaaaatgc gcagtatcga aagactgacg aggataattc
      961 agcgtaatgg atggcgaggg ctctatcgat ccgctactac gaacttttgg cgcagcgacg
     1021 cactccggca tcccttcccg cagccgtttc agccaggcat tcttaaaagg cgtaaagact
     1081 ttgtacatct cgtgattacc ggtcatcacc gcgccaggcg gcaggatcac gctgtcatca
     1141 aatccttcac acaccacgtt acgcagcgct ctttcaactt ccacatcccg cgcccgctca
     1201 ttcacttcat actgatagtt ataaaacagg tgggtaacgc tgttttccgc gcacacctgt
     1261 ttaactattt cgacactggc gacaaagtca tccacttcac ggaacaataa aggaatacct
     1321 ttttccgcaa gcgctatttg tagcccattc agttgagcat tgataagttc agcctgacgc
     1381 ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga tatacaacgc cagcacgcgt
     1441 gcagacgaat tgcggcaggc ggcagccagt gcgagattat cgtgcagacg taaatcttgg
     1501 cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg
     1561 gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc atgataaagc gccatatagt
     1621 gtttgatacg catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa
     1681 gcgcagccag ctgctggcgt tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt
     1741 gcatcaaaac attggtgtga tcgcgcgggt tggcggggtg agaaagcaaa acaactacgc
     1801 gttggcggta ctggttgtaa tagtcatcca gattttgcca ctggtgaata tcagccacga
     1861 acgggccaag tttgcggtac tccggctgag aatgcgccag aaagacgagt ttatagcggc
     1921 tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat
     1981 gaagggcaaa gatacggatt agggtgctgt catcaaatcg ttgtagattc attactgctc
     2041 ctgaacggta taaaggagta agtgtagacg atggtaaatg aattttatgg ggcagagttt
     2101 ttgcggggaa gtattttgtt ggtaaaaata ttatcaactt catctgagtg agaattattc
     2161 tactatttcc cgagctgcat tcattttttg tattttttat ttttagaaaa taacagttct
     2221 caaagcttaa ttacctcact attaattgta tttcaaacta acaatcgcac tccattttct
     2281 ctttttaaaa aacataaaca ctgttaaaac aaatacttat ttcgttatct taaatgctat
     2341 ttctacatca ctcgtcctta taatcaaatt attcttattc atagctattt ttgcaggttt
     2401 aaaatagcgg ctttcaccaa agtcagccat gaaaattaga ctacaagtca gggcaagatt
     2461 acgaaagccc gctccccgca aggactgacg ccagatagtt tctgtccatg gctgcttttc
     2521 gcatcttacg tc
//
LOCUS       DAJTXQ010000265         2530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000265 DAJTXQ010000000
VERSION     DAJTXQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.271"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..115
                     /gene="hokD"
                     /locus_tag="OO515_004209"
     CDS             <1..115
                     /gene="hokD"
                     /locus_tag="OO515_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6577967.1"
                     /translation="CLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            419..691
                     /locus_tag="OO515_004210"
     CDS             419..691
                     /locus_tag="OO515_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577968.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            765..1751
                     /locus_tag="OO515_004211"
     CDS             765..1751
                     /locus_tag="OO515_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6577969.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLHHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAKLSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            1752..2132
                     /locus_tag="OO515_004212"
     CDS             1752..2132
                     /locus_tag="OO515_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6577970.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            2129..>2530
                     /locus_tag="OO515_004213"
     CDS             2129..>2530
                     /locus_tag="OO515_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6577971.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          658 a          601 c          693 g          578 t
ORIGIN      
        1 ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacgaat
       61 tcgaaccggc cagacggagg tcgctgtctt cacagcttac gaacctgagg agtaagagac
      121 caggcggggg agaaatccct cgccacctct gatgggtcag gcatcctcaa tgcacccaca
      181 cttaacccgc ttcggcgggt ttttgttttt attttcaacg cgtttgaagt tttagatggt
      241 gccggaatag aatcaaaaat acttaagtag cgcgcaggga gaagagggat ggaccccgaa
      301 caggggagtg ctatttatct ggaaggattc tgttgatgaa aatcgaagaa ttacgtgaaa
      361 tttttagtga agatggcctc tatactgtgc gcgttgagaa tggcgctatt gtcagccact
      421 gccttccaga ctacacaatt ctgataattc agccgtcttt gccagcaggc acgggcggcg
      481 ctctcacgca tttaaatctg attggtacca gcatccccca tgcactgaag aacaggccga
      541 atggctcatt cagtgttacc gcaggcgcgg atgcgaggtt aaaaaagccc ttagcctcga
      601 ctaccgtcac tggataatct ccgtcaggct cccttactcc gaacggccaa cgcgtccgtc
      661 ccgcacattc cagcaacgga tctggaggta atgtgcgggt attacttcga cctgttctgg
      721 taccggaact cggtctggtt atcgttaagc caggccgtga atcaatgtca gcattccata
      781 acggcagaat actggtggag ccggaaccaa aaagcatgcg taatctgccg tccggggtcg
      841 ttcctgccgc tcgccagccg ctggtggaag acaaaacatt gctgccgttt ttcagtaacg
      901 cacgggtgat tcgtgctgct ggtggtgctg gtgcattgtc tgactggctg ttgcaccata
      961 ttaaatcctg ccagtggcca cacggcgatt atcatcacag cgaaaccgtc attcaccgtt
     1021 atggtaccgg cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgt gaccagacat
     1081 ccgaatcact cgagcaactt gctcatcaaa acctgtcagc atggatgatt gacgtcatcg
     1141 gtcacgcaat aagcggtacg caggagcgtg aattatctct ggctaaatta tcctggtggg
     1201 cggtccgcaa tcaggtggcg gacgcgctac cggaagcggt attacgtcgt tcgctggggt
     1261 tgcgtgcgga aaaaattcgc tctgtgtaca gtgaaagcga catcataccg ggagagcaga
     1321 cagccaccag catactgaag cagcgcacaa aaaatattgc gttactgcct cacgtccacc
     1381 agcaacagaa cccaccacag gaaaagacgg tggtcagcat tgccgttgat ccggagtcac
     1441 cggctcagta tctccagcgc cagaaatcac aacgggaaga gatgcctgta tacacgcgct
     1501 gggtaaaaac gcagaaatgc atgacgtgtg gcaatcaggc agatgatccg catcacatca
     1561 ttggtcatgg actgggaggg atgggaacaa aggctgatga tttgtttgtt attccgttat
     1621 gccgtaaatg ccgtaacgaa ctacatgccg gagtaaaaga ttttgaagag aaacacggca
     1681 gtcagctgtt gttgctgatt cgttttttaa tgcacgcgag aaattcgggt gttctgaagt
     1741 ggaaagcata aatgaccgag cgcatagaat ttgttttacc ttacccgccg acggtgaaca
     1801 cttactggcg tcgtcgtggc agcacatatt ttgtatcaaa agccggggag cgttatcgcc
     1861 gggcagtggc gcttattgtt cgccagcagc ggctgaaatt aagcctgtcc ggaaggttgg
     1921 caataaaaat tattgcagaa ccaccggata agcgccgccg tgacctggac aatattctga
     1981 aagcgccgct ggatgcgctg acgcatgcgg ggttgctaat ggacgatgag cagtttgatg
     2041 aaatcaatat cgttcgtgct cagccagtat ctggtggacg tctgggggtg aagatttatc
     2101 ccataatgct tgaagggcag gtcaaaaaat gaaactggaa gatttaccga aatactactc
     2161 cccaaaatcc cccggcctga ctgatgcatc ggcctcaacg tcgaaagatg cgctgagtat
     2221 cactgatgtg atggccgcgc agggcatgac acagaatcgg gctgagatgg gggtttctgc
     2281 gttccttggg aaaatgggca ttagtatgaa tgacagagag cgggcaacag aattgctgac
     2341 agaatatgca ctcagtcggt gtgatcgcgt ggcggcgtta agaaaactcc cggcagaaat
     2401 aaaaccggca gtgatgcgta ttatggcttc gtatgcgttt gaagattatg cccgtagcgc
     2461 ggcgagcaaa aaacagtgcc cctgctgtca cggaaaaaaa tttattgaaa gcgaggtttt
     2521 tacaaacaag
//
LOCUS       DAJTXQ010000266         2447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000266 DAJTXQ010000000
VERSION     DAJTXQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.272"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..316)
                     /locus_tag="OO515_004214"
     CDS             complement(26..316)
                     /locus_tag="OO515_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6577972.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="OO515_004215"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="OO515_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6577973.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="OO515_004216"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="OO515_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6577974.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="OO515_004217"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="OO515_004217"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2447)
                     /locus_tag="OO515_004218"
     CDS             complement(2172..>2447)
                     /locus_tag="OO515_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6577975.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          576 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgacc
//
LOCUS       DAJTXQ010000267         2276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000267 DAJTXQ010000000
VERSION     DAJTXQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.273"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="OO515_004219"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="OO515_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6577976.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="OO515_004220"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="OO515_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6577977.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="OO515_004221"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="OO515_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6577978.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..>2276
                     /gene="cas6e"
                     /locus_tag="OO515_004222"
     CDS             2131..>2276
                     /gene="cas6e"
                     /locus_tag="OO515_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6577979.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRR"
BASE COUNT          565 a          568 c          610 g          533 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcg
//
LOCUS       DAJTXQ010000268         2254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000268 DAJTXQ010000000
VERSION     DAJTXQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.274"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1548
                     /locus_tag="OO515_004223"
     CDS             <1..1548
                     /locus_tag="OO515_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR6577980.1"
                     /translation="VASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVCIGIFKY
                     NLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRNQLLASP
                     SVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSLETLTRT
                     ITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVISDGNGVL
                     LPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVLGNDPAY
                     GMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQLHNRLS
                     SMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKDHILLAA
                     DVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDSVPVDPNML
                     NQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEVPDELEL
                     KIMGALM"
     gene            complement(1671..2168)
                     /gene="rayT"
                     /locus_tag="OO515_004224"
     CDS             complement(1671..2168)
                     /gene="rayT"
                     /locus_tag="OO515_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6577981.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
BASE COUNT          532 a          596 c          634 g          492 t
ORIGIN      
        1 gttgccagcg aggccgattt ctacggcgcg atggacgggg catcgaaatt tgtgcgcggg
       61 gatgctatcg ccgggatgat gattctggcg atcaacctga tcggcggcgt ctgtatcggg
      121 atcttcaaat acaacctgag cgccgacgcc gccttccagc agtatgtgct gatgaccatc
      181 ggcgacgggc tggtggcgca aatcccttcc ctgctactct ccaccgcggc ggcgattatc
      241 gtcacccgcg tcagcgacaa cggcgatatc gcccatgacg tgcgtaacca gttgctggca
      301 agcccatcgg tgctctacac cgctaccgga attatgttcg tactggcggt agtgccggga
      361 atgccacatc tgccgttttt gctgttcagc gccctgcttg gctttaccgg ctggcggatg
      421 agcaaacgcc cgcaggcggc ggaagcggaa gagaaaagtc tcgaaacgct gacccgcacc
      481 atcaccgaaa ccagcgagca acaggtcagt tgggaaacca ttccgctgat cgagcccatc
      541 agcttaagcc tcggctacaa gctggtggcg ctggtggaca aagcccaggg caacccgctc
      601 acccagcgta ttcgcggcgt gcgacaggtg atatccgacg gcaacggcgt gctgctgccg
      661 gagatccgca ttcgggaaaa cttccgcctt aagcccagcc agtacgctat tttcatcaac
      721 ggcattaagg ctgatgaagc ggatattccg gcggataaac tgatggcgct gccctccagc
      781 gaaacctacg gcgagataga cggcgtgctg gggaacgacc cggcgtacgg gatgccggtg
      841 acctggatcc agcctgcgca gaaagcgaag gcgctgaata tggggtatca ggtgatcgac
      901 agcgccagcg tgattgctac ccatgtaaat aagattgtgc gcagctatat tcctgattta
      961 tttaactatg acgatattac gcagttgcat aaccgtttgt cgtcgatggc accgcgcctg
     1021 gcggaggatt taagcgcggt gctcaattac agccagttgc tgaaagtgta ccgggcgctg
     1081 ctgaccgaag gcgtttccct gcgcgatatc gtcaccatcg ccaccgtgct ggtcgccagt
     1141 agcgcggtga ccaaagatca tattctgctg gcagccgatg tgcgcctggc gctgcggcgc
     1201 agcattaccc atccgttcgt tcgcaagcag gagctgacgg tgtataccct gaataatgag
     1261 ctggaaaatc tgctgactaa cctagtgaat caggcgcaac agggcggtaa agtgatgctc
     1321 gacagcgtgc cagtggaccc gaatatgctc aaccagttcc agagcacgat gccacaggtg
     1381 aaagagcaga tgaaagcggc ggggaaagac ccggtgctgc tggtgccacc gcagctgcgc
     1441 cctttgctgg cgcgttatgc aaggttgttt gcgccggggc tgcatgtgct gtcgtataac
     1501 gaagtgccgg acgagctgga gttgaagatt atgggggcgt tgatgtaagt atccggcatt
     1561 ggtgcacaac tgccggatgc ggcgtaaacg ccttatccgg cctaccgttc aggcacaagt
     1621 ttgtaggcat gataagacgc gccagcgtcg catcaggcat ctgcgcataa ttataaaatg
     1681 atacgctcgc cagcatttat atccgttacg tcccccgccc aatcgatggg atataaccct
     1741 cgcgcgacat cgcgatggaa cgttgagaat ggccaatcac tcacttgctt tacccaacca
     1801 tgctttactg gatttatata aatataatca acatgatgcc tataatcttt ggtattgcgg
     1861 atggcgtgct cccaaaaacg tggttgccag atatttttca atccacaagc atgggtaaat
     1921 tgctttttaa tttcccgcca gcgagaggaa aaatcatcat cgccttcagg taatgtccat
     1981 atgcaatgca tatgctctgg caaaacgacc caggcgttga tttcaaaagg cctgtctcgc
     2041 ttaactttaa taatggcgtt acggagcatc tggtactggg tggtcaagag ttggcttcga
     2101 cgattgcgta aattcaccgt gaaaaaccag gttcccccct tgatgtaata acgacgatat
     2161 tcagacatgg cgactccttt ttctcaacgg taagaagaaa aggaaaggtc ggcaatgagg
     2221 tgatggaaca gggcaagatt acgaaagccc gctc
//
LOCUS       DAJTXQ010000269         2230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000269 DAJTXQ010000000
VERSION     DAJTXQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.275"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..498)
                     /locus_tag="OO515_004225"
     CDS             complement(<1..498)
                     /locus_tag="OO515_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6577982.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGV"
     gene            813..1865
                     /locus_tag="OO515_004226"
     CDS             813..1865
                     /locus_tag="OO515_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR6577983.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          693 a          460 c          503 g          574 t
ORIGIN      
        1 tacaccgcta ctgtaaaagg cttttttatt tttcggtgca ctttcaacgg aaagcaacgc
       61 cgcgcctccc cattcgccgc cgacggcaaa tccctgaatg acacgcagtg tcaccagtaa
      121 aataggtgcc caccacccaa tggtcgagaa tgaaggaaga ataccaatca aggctgtcgc
      181 gatgcccatc atccagacgg tcagcattaa catgcgctta cgtcccagtc ggtcgccaaa
      241 gtgaccgaaa atgacaccgc cgagcggacg gaaaaggaat ccgacgccaa aggtagcaaa
      301 tgcggcgagc gttcccatcg ccgggcttac ttgcgggaaa aactcgcgat taaacaccag
      361 tgcggcggtg atgccataga gtaaaaaatc ataccagtcg acgacggcac cagcgaagct
      421 gcctaacgca gctcgtcggg cgcgacttaa cgaaagcgtc ccttcatcgg gacgagtgga
      481 gatgagcgtg gagtccataa tgatcctgtt tgtactgtct ttttataatt agtatggaaa
      541 aggtaactca cattgccgtc gatctataaa cgaaccttat gagactacct cgacagagcg
      601 catctgcaaa tgattttatg gctgattagc ggcaacatga aaaattcgat ggaagatgaa
      661 agcgcaggag ttgctggtgt tgtttaagga ttaagcagag gacccacagg ttagagatat
      721 gaaacataaa agtccgcata tgcacaaaat ggcaccctga tgagtctgat agactgcatt
      781 gcattataac cagtagggag gcgaggttag gtatgttttt agcatcattg ttgagacgta
      841 ttgcatttag ttactacgat tataaagctt ataatttcaa tattgaaaaa acagactttg
      901 ttgtcatcca tattcccgat cagattggcg atgctatggc catctttcct gttattcggg
      961 cgcttgaatt gcataaaatt aagcatcttt taattgtaac gtcgacaatt aatttagaag
     1021 tctttaatgc gcttaaactt gaacagacta aattaacatt agtcacaatg actatgcagg
     1081 atcacgcaac attaaaagaa ataaaagatt tagcaaagaa cataacacag caatacggta
     1141 cgccggatct ttgtattgag gggatgcgta aaaagaacct gaaaacgatg ttatttatca
     1201 gtcagttgaa agcaaaaacg aattttcagg ttgttggtat aaccatgaat tgctactccc
     1261 ctttgtgcaa gaacgcgtcc cgtatggatc agaaactccg ggctcccgta cctatgacat
     1321 gggcatttat gatgcgtgag gcgggttttc cagcagtcag gccaatatat gaattgccac
     1381 taagtgagga tgtactcgat gaggtgcgcg aggaaatgcg atcgttagga tcttacattg
     1441 cgttcaattt agaaggtagc tcgcaggaac gtacattttc attatcgatt gcagaaaatc
     1501 taatagcaaa aattcaaagt gaaacagata taccaatagt gatcgttcat ggacccaaag
     1561 gtgaagataa agccagggca ttagtggatt gttataataa tgtctaccgt ttatccttac
     1621 caccctcgat taaacgttca gcagcaatca taaaagatgc ttatatcgca ataactcctg
     1681 acacctcaat attacatatg gcaagtgcct ataatacccc tgttgttgca atttatgctg
     1741 attacaaaac gcgatggccc gcaatggccg atgtctcgga gtcagtcgtc gttgggcaaa
     1801 aaattgacaa tataagtctg gatgaattcg caaaggcatt aaaaagtgtt ttggcgagaa
     1861 tatgagacag tacttatgca tagtaatgcc gatctgttat gaacggtggt gtcatcaata
     1921 ctctgaggct ggtcaatcat cacagaaaac cagcctttga atcaaagcag tactcaccgg
     1981 atacgatcat agtcacatcg aacgcaattc tggcggtgcc gcatttttat ttattcattt
     2041 tctgtgagag aagggcaagc tctatagagg gaatgtaaaa aagagagctc cgaatggagc
     2101 actctttaag aaattataac ggaaaatcag aggtttttat aacatgtcgc ataagcaaac
     2161 ccaagctttt caaatacgct ttgatcacct gttgccaggg aaactacgta ttgttcgctt
     2221 gagtttgcgg
//
LOCUS       DAJTXQ010000270         2197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000270 DAJTXQ010000000
VERSION     DAJTXQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.276"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..448)
                     /locus_tag="OO515_004227"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="OO515_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(501..1097)
                     /locus_tag="OO515_004228"
     CDS             complement(501..1097)
                     /locus_tag="OO515_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6577984.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1124..1726)
                     /gene="yadL"
                     /locus_tag="OO515_004229"
     CDS             complement(1124..1726)
                     /gene="yadL"
                     /locus_tag="OO515_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCR6577985.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(1741..>2197)
                     /locus_tag="OO515_004230"
     CDS             complement(1741..>2197)
                     /locus_tag="OO515_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6577986.1"
                     /translation="CNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLV
                     ASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKP
                     NSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          600 a          487 c          441 g          669 t
ORIGIN      
        1 cggtaccgta agctgaccac atgtttgaaa ttaataatgt gtaatacaaa ccaggaactg
       61 atgtattgaa taatttatgg gagccataca ttttacctga gtcaaccatt gcattttcaa
      121 tacgcattgc gctaccactt gtgctatcac ttctgttgca ataaagaaaa ccaccaccag
      181 aagcagtatc taactgactc tactgtaatt ttacacctgt aggtccaagc tgcgtcggtt
      241 tgtagaaaac taaattattt gtacttgttg catatctgaa tatgacagta gcatcgccat
      301 tcggccccgt ttctctccat ttttgatctg acggaccaac gccgtaacta ttcccaacaa
      361 tatatcctac gttatcatca gtgccagcac tcgcggtata acaactcaag cacagaatta
      421 aaaaaaaatg tctgaagaat ttcttcatta tcactccacc atctactgct gtaacagtcg
      481 cagttatttg ttttgatcta ttattcgtag tacacatcaa ctaatatatg actcattact
      541 tggccagata tgacatccag cgcgggatcg attttttgca ttctggcata aaatttccat
      601 gaagaattat gcagggcaga agtcattact ggatacacgg aacgagaatt tcctgctgaa
      661 ctaataacat tgaatatatt ggtcgggtcc tgaatggaaa agagaacgac accgacattt
      721 ttagccccgg ttgcgacttc attggcaaat atttgccttg aacctgcagc catgacaccg
      781 ctaagggctc taaaatcaat atgtaactga ttaagcacat cgccagttcc ctgtaatgca
      841 cagtctgata ctgtgacagt gaactctttt ccacctggtt gatagctttc aggagtaaca
      901 ttatctgaaa aatatcccac cccaacgacg ccaagatcaa tattaccctg gttactgatt
      961 ccgcttttgc aagtgctgtt ttttacattg gcaacaaggt caatatcctg cccagcgatg
     1021 gctggatagg taatgggaaa ctgtagtgac agaaacagaa ttattctctt catcagctta
     1081 cgttgagtgg gatgcatata tagttcctgg atctgtcagt ctgttagtta taggttactg
     1141 taaatgtggc aggtgcagaa aatttacccg cagtgacatt gcttacggtt ttattttttt
     1201 cgacgaccag tcgagcactc atcggataat aaacaaccgc gttggctgca ttggagatag
     1261 tgacctcttg agatgctggt gtcacacaac taaattgtgt tgcgctccct gttgccggag
     1321 tttcggtgct ccagacttca acggcgacgg cacttgcttt atctgcggct gtggatccat
     1381 ttgcgagccc cgcaccgtca ttagcagtac cttcgcatgt ggctcgcttg gttaatttta
     1441 tttgtgcttt tttattgggg atacccgcac agtctttgaa tttgagtttg aatgttttaa
     1501 ctttggtctt ggcattgatt tcagaaatat atacatcgcc aaagtcaaca acagaaatgt
     1561 cctgatcaga gtcatttact aatgtagcgg tacaagtacc tatttctacg gtagtaatca
     1621 cggtcagccc aatgctgtct gttgccgcat aagagggtac gctgaacaat gaggcagcca
     1681 aaacgacact gctggataat ccataacgta aatttttaaa tgtcatctta attatccttc
     1741 ttattgatag atgaaattaa acgttgccag tgcacgaaaa ttccccggta cgccttgccc
     1801 tgcatctgtt tcacgtagcg caacggtcat ttcaagaccc ttatcgggct gcatctcgtc
     1861 tgtgctccag cgtatctttt ccgcactgtt aggtttaagg aaagtggcat catcagtagt
     1921 ccgttttttg aaacccatac cgatattact tgtcgtcgaa gatgaatcac cagactgcgg
     1981 tataataagc ttaggtgagc ttgatgatgc atttccggtc agagtggtat caatccaact
     2041 gatgccactg ctgcacccac tagcaaccag tttaaaatca gcctgagatt ctgtcgtttt
     2101 attcgcgatc ttatccagac ccatcttagg gattctcaat gtgtagttat tattgccatc
     2161 attcgtgacg ttattaccag taagtgtgat gttacag
//
LOCUS       DAJTXQ010000271         2118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000271 DAJTXQ010000000
VERSION     DAJTXQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.277"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..84)
                     /locus_tag="OO515_004231"
                     /pseudo
     CDS             complement(1..84)
                     /locus_tag="OO515_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            50..835
                     /gene="lafU"
                     /locus_tag="OO515_004232"
     CDS             50..835
                     /gene="lafU"
                     /locus_tag="OO515_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6577987.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            906..1961
                     /gene="dinB"
                     /locus_tag="OO515_004233"
     CDS             906..1961
                     /gene="dinB"
                     /locus_tag="OO515_004233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6577988.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            1958..>2118
                     /locus_tag="OO515_004234"
     CDS             1958..>2118
                     /locus_tag="OO515_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6577989.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLA"
BASE COUNT          542 a          534 c          585 g          457 t
ORIGIN      
        1 gtccagggta aagcgggcag aaacctcgga gatacgctcg gcccctttgg tgacgacaat
       61 aaagttgatt gtcaacagcg tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca
      121 cgggcagtcg atttttaacg gtggcgggct gtcgccgtta aataaaatca gcccgtctca
      181 tccgccaaaa cctgccaccg tggcggtgcc ggaagaaacg gagaaaaaag cgcgcgacgt
      241 caatgagaaa acggcgctgc tgaagaagaa atccgccacc gagcttggcg agctggcaac
      301 cagtatcacc accattgccc gtgatgcgca tatggaagcg aatctggaga tggagattgt
      361 tccccaggga ttacgcgtgc tgattaaaga cgaccagaat cgcaatatgt tcgaacgcgg
      421 cagcgcgcag attatgccgt tctttaaaac gctgctggtg gagctcgcgc cagttttcga
      481 ctcgctcgat aacaaaatta ttattaccgg gcataccgat gcgatggctt acaaaaacaa
      541 tatctacaac aactggaacc tttcgggcga tcgcgcgctt tcggctcgtc gggtgctgga
      601 agaggccgga atgccggaag ataaagtgat gcaggtaagc gcaatggcgg accagatgct
      661 gctggattcc aaaaatccgc aaagcgcggg caaccggcgc attgagatta tggtgctgac
      721 caaaagtgcg tccgatacgc tgtatcaata ctttggtcag catggggata aagtggtgca
      781 gccgctggtg caaaagctgg ataagcagca ggtgctttcg cagcgaacgc gttaaatgct
      841 gaatctttac gcatttctca aactctgaaa tcactgtata ctttaccagt gttgagaggt
      901 gagcaatgcg taaaatcatt catgtggata tggactgctt tttcgcagcg gtggagatgc
      961 gcgacaatcc cgccctgcgc gatatcccta ttgctattgg cggcagccgc gaacgtcggg
     1021 gggtgatcag taccgccaat tatcccgcgc gtaaatttgg cgtacgtagc gctatgccga
     1081 cagggatggc gctcaaatta tgcccgcatc tcaccttgct tccggggcgc tttgacgcct
     1141 acaaagaagc ctcaaatcat atccgcgaaa tcttctcgcg ctacacctcg cgtattgaac
     1201 cgttgtcact ggatgaggct tatctcgacg tcaccgatag cgtccattgc cacggttctg
     1261 cgaccctcat cgcccaggaa atccgccaga cgattttcaa cgagctgcaa ctgacggcgt
     1321 ctgcgggcgt ggcacccgta aagtttctcg ccaaaatcgc ctccgacatg aataaaccca
     1381 acggccagtt tgtgattacg ccggcagaag ttccggcatt tttacaaacc ttaccactgg
     1441 caaaaatccc cggcgtcggc aaagtctcgg cggcaaaact ggaagcgatg gggctacgaa
     1501 cctgcggtga tgtacaaaag tgtgatctgg tgatgctgct taaacgcttt ggcaaatttg
     1561 gccgcatttt gtgggagcgt agtcagggga ttgacgagcg cgacgttaac agcgaacggt
     1621 tgcgaaaatc cgtcggcgtg gaacgcacga tggcggaaga tatccaccac tggtctgaat
     1681 gtgaagcgat tatcgagcgg ctgtatccgg aacttgaacg ccgtctggca aaggtaaaac
     1741 ctgatttact gattgcccgc cagggggtga aattaaagtt cgacgatttt cagcaaacca
     1801 ctcaggagca cgtctggccg cggctgaata aagctgactt aatcgccacc gcgcgtaaaa
     1861 cctgggatga acgccgcggc gggcgcggtg tgcgactggt ggggctgcat gtgacgttgc
     1921 ttgatccgca aatggaaaga caactggtgc tgggattatg aataacatac aaataagaaa
     1981 ctatcagcct ggcgattttc agcaactatg cgctattttc attagagcgg ttacgatgac
     2041 cgccagtcag cattattctc cacaacaaat tgccgcctgg gcgcagattg acgaatctcg
     2101 ctggaaggag aaactcgc
//
LOCUS       DAJTXQ010000272         2102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000272 DAJTXQ010000000
VERSION     DAJTXQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.278"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="OO515_004235"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="OO515_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6577990.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..2102
                     /locus_tag="OO515_004236"
                     /pseudo
     CDS             1552..2102
                     /locus_tag="OO515_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          504 a          539 c          667 g          392 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 gg
//
LOCUS       DAJTXQ010000273         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000273 DAJTXQ010000000
VERSION     DAJTXQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.279"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1118..1453)
                     /locus_tag="OO515_004237"
     CDS             complement(1118..1453)
                     /locus_tag="OO515_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCR6577991.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1582..1929
                     /locus_tag="OO515_004238"
     CDS             1582..1929
                     /locus_tag="OO515_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577992.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1949..>2095)
                     /locus_tag="OO515_004239"
     CDS             complement(1949..>2095)
                     /locus_tag="OO515_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577993.1"
                     /translation="GSTCLQLWNKEGWVVRREGDPLEVAQWLPACHDAGMEVRVQINE
                     SAAP"
BASE COUNT          658 a          438 c          378 g          621 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg
      481 gggattacat ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt
      541 aatataagct tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt
      601 atattataaa tataacataa tacatctata tggggtgtac atatccccac ataaaaacac
      661 atactctcat taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc
      721 gttcttgtac tatggtttag tattctcaag tacttacaga gaaagaatag catattacat
      781 cctttatttt cactaatcct gacaccacta accattttaa tcattagact tatgatagat
      841 ttaacataca accaataaat tataaacaaa ataaaaatag ggaacattat atgctttata
      901 aaattttcac attaaacaca aagataaaaa caactatcat tacaatatgt gcattatcat
      961 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
     1021 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
     1081 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
     1141 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
     1201 tataaacagc aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga
     1261 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
     1321 aaaccattcc gcagagagaa atacaccaga acagtaatct gtatttaaaa tcataatccc
     1381 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
     1441 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
     1501 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
     1561 tcatggaacg gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg
     1621 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
     1681 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
     1741 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
     1801 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga
     1861 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat
     1921 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
     1981 gaacctccat tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc
     2041 cctcccggcg taccacccag ccttctttat tccataactg cagacaggtg ctgcc
//
LOCUS       DAJTXQ010000274         2059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000274 DAJTXQ010000000
VERSION     DAJTXQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.280"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..306)
                     /locus_tag="OO515_004240"
     CDS             complement(<1..306)
                     /locus_tag="OO515_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCR6577994.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     "
     gene            complement(415..1968)
                     /gene="caiC"
                     /locus_tag="OO515_004241"
     CDS             complement(415..1968)
                     /gene="caiC"
                     /locus_tag="OO515_004241"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6577995.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          489 a          543 c          487 g          540 t
ORIGIN      
        1 gttcacagct gcgataaccg gtttgtcgag attgaaaatt tcggttaatc ccgcaaatcc
       61 acccgggcca aagtcagcat ccggtgcttc gccttctgct gccgctttta aatcccagcc
      121 cgcggaaaag aacttctctc cggcaccggt aataatggcg acacgtaatt gcggatcgtc
      181 acggaaattt agaaatactt cgcccatttc aaagctggtt tttgcatcaa tagcattcgc
      241 ttttggacga tcaagggtaa tttccagaat tgatccattg cgggtcagat gtaatgattc
      301 actcattcct tttctccatt tttgcttttt cagggacgac aacatccctg caaaaaatgc
      361 atattgtttt atagcgtgat tattagttgg cagggtagtt ccctgctgtt tcatttattt
      421 cagattcttt ctaattattt tccccgagca attacgtggc agatcttttc tgatctccag
      481 ataagagggc actttaaatt tcgccatatt ttgttcgcag aagcggaaaa attcctcttc
      541 gctcaatgtt tcaccttcat tcagcaccac aaatgctttg atggcttcat cgcgaatcga
      601 atctttaata cccacaacca cgatatcctg aatttttgga tgggtggcga tgatattttc
      661 cagctccacg caggagacat tctcgccacc gcgtttgatc atattgcagc ggcgatcgac
      721 gaaataaaaa aagccctctt cgtcgcagta tccggtatcg ccggtatgta gccagccgtc
      781 ggcttccagc actttcgcag tggctttcgg gttgagaaaa tactctttga agatggtttt
      841 ccctggtacg cctttaatac agatttcacc gatctcacct gccgggagcg ggcgattgtg
      901 atcgtcgcgg atctccgctt cgtagcaaaa ccccgcccga ccaatcgacg gccagcgtcg
      961 tttatcgcca gggcgatcgc cgataatgcc cacaatggtt tccgtcatcc cataagacgt
     1021 cagcaagcga acgccgaagc gttcacaaaa cgcatctttt tcctgctccg acaagttgag
     1081 ataaaacatc acttcccgca ggcggtgttg ccgatcgttc gctgaaggcg gctgcgccat
     1141 caaggtacgg atcatcatcg gaatacattc ggtaacggtg gcgcggtact tctgtacctg
     1201 tccccagaag gcgcgggcgc tgtatttctc gaccagcaca aaggtggccc cggcagaaaa
     1261 cgccgccatc gccgcagtac actggcaatc gatatgaaac gcaggcatta ccgtcaggta
     1321 gacgtcatcg tcacgcagtg cacactgcca ggcggagtaa tatccagcga agcgcaggtt
     1381 gtaatgggta atcaccacac ctttcggtcg ggaggtggtg ccggaggtga agagaatttc
     1441 cgccgtatcg tcagtcgata gcggcggtgc atagcacaag gtggcaggtt gttgattttt
     1501 cagttgagta aacgaactca cgccatcatc agcgggaagt gccacatctg tcaggcaaat
     1561 gtgccgcaat tgagtggcat cttcctgctg aatctgttga tacataggat agaattgcgc
     1621 actggtcacc agcaggcacg cctggctatt ttgcaggatc cacgcgcttt cctcgcgcaa
     1681 caggcgggcg ttaatcggca ccataatcgc gccaattttt gccagcccga accagcaaaa
     1741 gataaattcc gggcagttgt cgagatgtag agcaaccttg tcgcctttgc gaatccccag
     1801 cgtataaaac aggtttgtcg tgcggttaat ctcctgattt aactcaagat aactataccg
     1861 gttaacgact ccgccgctgg attcacaaat cagcgccgtt ttatgaccgt aaacgtccgc
     1921 cagatcgtcc cacatttgac gtagatgttg tccgccaatg atatccattg cacctctatc
     1981 catttttgtt cgtttgttat tgggcgggcg ctagtcaggc aagccgactg acgccacgcg
     2041 tttagtcctc aactttggc
//
LOCUS       DAJTXQ010000275         1981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000275 DAJTXQ010000000
VERSION     DAJTXQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.281"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..116
                     /locus_tag="OO515_004242"
     CDS             <1..116
                     /locus_tag="OO515_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR6577996.1"
                     /translation="TLNCGEGVRFAADVTHIYRNGGEQTVHFHSLIHYPRS"
     gene            189..>1981
                     /gene="rlhA"
                     /locus_tag="OO515_004243"
     CDS             189..>1981
                     /gene="rlhA"
                     /locus_tag="OO515_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6577997.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQL"
BASE COUNT          493 a          489 c          522 g          477 t
ORIGIN      
        1 agacccttaa ttgtggggaa ggcgttcgat ttgctgcaga cgtcacgcat atctatcgta
       61 atggtgggga gcaaaccgta cattttcatt ccctcatcca ttacccgcgc agttaagcgg
      121 gaaaactatt tcgcaacgtc gcgcttgtgg ctaaaatagc cgccattttt cagctactgg
      181 ataagaatgt gaccgtatct tctcatcgac ttgaactgtt aagcccggca cgcgatgccg
      241 ccattgcccg cgaagctatt ttgcacggtg ccgatgctgt ttatatcggc ggccctggtt
      301 ttggtgcccg tcataatgcc agtaatagcc tgaaagatat tgccgagctg gtgccgtttg
      361 cccatcgtta tggtgcaaaa attttcgtca cgcttaacac cattttgcat gatgatgagc
      421 tggaacccgc gcaacggctg attactgacc tctaccagac cggtgtcgat gcgctgattg
      481 ttcaggatat ggggattctg gaacttgata ttccgccgat tgaactgcac gccagtacgc
      541 agtgcgacat tcgtacagtt gaaaaagcga agttcctctc tgatgttggc ttcacgcaga
      601 ttgtgctggc gcgagagctg aatcttgatc agatccgcgc gattcaccag gctacggacg
      661 cgaccattga attctttatt catggggcac tgtgcgtggc ctattcgggt cagtgctaca
      721 tttctcatgc gcaaacaggg cgtagcgcca accgtggcga ttgctcgcag gcgtgccgtt
      781 tgccatacac attgaaagac gatcaggggc gggtggtttc ctatgaaaaa catctgctgt
      841 cgatgaaaga taacgatcag actgccaacc tcggcgcgct gattgatgct ggcgtacgct
      901 ccttcaagat tgaagggcgt tacaaagata tgagctacgt gaagaatatc accgcccatt
      961 atcgccagat gctggatgcc attattgaag aacgtggcga tctgacgcgt gcttcatcag
     1021 gtcgtactga acatttcttt gttccatcga cggaaaagac tttccaccgt ggtagcacag
     1081 attattttgt gaatgcccgt aaaggcgata ttggcgcgtt cgattcgccg aaatttatcg
     1141 gcctgccggt gggtgaagta ttgaaagtgg cgaaagatca tctcaatgtt gccgtcaccg
     1201 agccactggc aaatggtgat ggcctgaacg tgatgattaa acgtgaagtc atcggttttc
     1261 gtgccaatac ggtcgagaaa accggagaaa atcagtaccg cgtctggccc aatgaaatgc
     1321 cagcagattt gcacaaaatt cgtccacatc acccactaaa ccgtaatctt gatcataact
     1381 ggcagcaggc actgacaaaa acctccagcg aacgtcgggt ggcggtagac attgaactgg
     1441 gcggctggca ggaacaactg attctgaccc tcaccagtga agagggtgtc agcatcacgc
     1501 atacgctgga cgggcagttc gacgaagcca ataacgccga aaaagcaatg aacaatctga
     1561 aggatggtct ggcaaaactg gggcaaaccc tctattacgc ccgcgatgtg caaattaatt
     1621 tgccgggggc gctgtttgta ccaaacagtc tgttaaacca gttccgccgt gaagctgctg
     1681 acatgctgga tgctgcgcgt cttgccagtt accagcgcgg cagccgtaaa ccggttgctg
     1741 atcctgcgcc ggtttttccg caaacgcatc tgagtttcct cgcgaacgta tacaaccaga
     1801 aagcgcgtga attttatcat cgctatggtg tgcagctgat tgacgcggcg tatgaggcac
     1861 atgaagagaa gggcgaagtc ccggtgatga tcaccaagca ttgtctgcgc tttgccttta
     1921 atctgtgccc gaaacaggcg aaaggcaata tcaaaagctg gaaggcgacg ccaatgcaac
     1981 t
//
LOCUS       DAJTXQ010000276         1887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000276 DAJTXQ010000000
VERSION     DAJTXQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.282"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO515_004244"
     CDS             <1..389
                     /locus_tag="OO515_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6577998.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO515_004245"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO515_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6577999.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OO515_004246"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OO515_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6578000.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OO515_004247"
     tRNA            complement(1806..1881)
                     /locus_tag="OO515_004247"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
BASE COUNT          503 a          446 c          457 g          481 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattag
//
LOCUS       DAJTXQ010000277         1870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000277 DAJTXQ010000000
VERSION     DAJTXQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.283"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OO515_004248"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OO515_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCR6578001.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1870
                     /gene="istB"
                     /locus_tag="OO515_004249"
     CDS             1253..>1870
                     /gene="istB"
                     /locus_tag="OO515_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6578002.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGE"
BASE COUNT          473 a          462 c          533 g          402 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa
//
LOCUS       DAJTXQ010000278         1812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000278 DAJTXQ010000000
VERSION     DAJTXQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.284"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..382
                     /gene="mhpC"
                     /locus_tag="OO515_004250"
     CDS             <1..382
                     /gene="mhpC"
                     /locus_tag="OO515_004250"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6578003.1"
                     /translation="RQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENF
                     VKSLEANPKQFPDFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRD
                     CGHWAQWEHADAFNQLVLNFLARP"
     gene            392..1201
                     /gene="mhpD"
                     /locus_tag="OO515_004251"
     CDS             392..1201
                     /gene="mhpD"
                     /locus_tag="OO515_004251"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR6578004.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            1198..>1812
                     /gene="mhpF"
                     /locus_tag="OO515_004252"
     CDS             1198..>1812
                     /gene="mhpF"
                     /locus_tag="OO515_004252"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6578005.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLM"
BASE COUNT          399 a          467 c          532 g          414 t
ORIGIN      
        1 tcgtcagccg actatcgaaa acctgaagct gatgatggat atcttcgttt tcgataccag
       61 cgatttgacc gacgccctgt ttgaagcgcg cctgaataat atgctgtcgc gccgcgatca
      121 cctggaaaac ttcgttaaga gcctggaagc taatccgaaa cagttcccgg attttggccc
      181 acgtctggcg gaaatcaaag cgcaaaccct gattgtctgg gggcgcaacg accgctttgt
      241 gccgatggat gcgggtctgc gtctgctgtc cggcattgcc ggttctgaac tgcatatctt
      301 ccgcgactgt ggtcactggg cgcagtggga acatgccgac gctttcaatc aattggtgct
      361 gaatttcctc gcacgccctt aaggaatggt catgacgaag catactcttg agcaactggc
      421 ggcggattta cgccgcgccg cagagcaggg cgaagcgatt gcgccgctgc gcgatctgat
      481 tggtatcgat cacgctgaag cggcctacgc cattcagcac ataaatgtgc aatatgacgt
      541 tgtgcagggg cgtcgcgtgg tagggcgtaa agtgggcctg acgcatccga aagtgcaaca
      601 acaactgggc gttgatcaac cggattttgg gacgttattt gccgacatgt gttatggcga
      661 taacgaaatc attccttttt cccgtgttct gcaaccccgc attgaagcgg agatcgcact
      721 ggtgttgaac cgcgatttac ccgcaactga tatcaccttc gacgaactgt ataacgccat
      781 tgaatgggta cttccggcgc tggaagtggt ggggagccgc attcgcgact ggtcgattca
      841 gtttgtcgat accgtggcag ataacgcttc ctgcggggtg tatgtcatcg gcggtccggc
      901 gcaacgtccg gcggggttag acctgaaaaa ctgcgccatg aagatgacgc gtaataacga
      961 agaggtttcc agcgggcgcg gcagcgaatg cctgggacat ccgcttaatg ccgccgtctg
     1021 gctggcacgc aaaatggcca gtctgggtga accgctgcgc gccggagata tcattcttac
     1081 cggggcatta ggcccgatgg tggcggtgaa tgcgggcgat cgttttgaag cccatattga
     1141 aggcataggt tcagttgctg cgacattttc aagcgcagcc ccaaaaggaa gtctgtcatg
     1201 agtaagcgta aagtcgccat tatcggttct ggcaacattg gcaccgatct gatgattaaa
     1261 attttgcgtc acggtcagca tctggagatg gcggtgatgg tcggcattga tcctcagtcc
     1321 gacggtctgg cgcgtgccag acgtatgggc gtcgccacca cccatgaagg ggtgatcggg
     1381 ctgatgaaca tgcctgaatt tgctgatatc gacattgtat ttgatgcgac cagcgcgggc
     1441 gcgcatgtga aaaacgatgc cgctttacgc gaagcgaaac cggatattcg cttaattgac
     1501 ctgacgcctg ctgccatcgg cccttactgc gtgccggtgg ttaacctcga ggcgaacgtc
     1561 gatcaactga acgtcaacat ggtcacctgc ggcggccagg ccaccattcc aatggtggcg
     1621 gcagtttcac gcgtggcgcg tgttcattac gccgaaatta tcgcttctat cgccagtaaa
     1681 tctgccggac ctggcacgcg tgccaatatc gatgaattta cggaaaccac ttcccgagcc
     1741 attgaagtgg tgggcggcgc ggcaaaaggg aaggcgatta ttgtgcttaa cccagcagag
     1801 ccaccgttga tg
//
LOCUS       DAJTXQ010000279         1799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000279 DAJTXQ010000000
VERSION     DAJTXQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.285"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1138
                     /locus_tag="OO515_004253"
     CDS             <1..1138
                     /locus_tag="OO515_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6578006.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="OO515_004254"
     CDS             1150..1599
                     /locus_tag="OO515_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578007.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          544 a          297 c          503 g          455 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattacct
//
LOCUS       DAJTXQ010000280         1785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000280 DAJTXQ010000000
VERSION     DAJTXQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.286"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(37..273)
                     /locus_tag="OO515_004255"
     CDS             complement(37..273)
                     /locus_tag="OO515_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6578008.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="OO515_004256"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="OO515_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6578009.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..>1785)
                     /gene="bglJ"
                     /locus_tag="OO515_004257"
     CDS             complement(1240..>1785)
                     /gene="bglJ"
                     /locus_tag="OO515_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6578010.1"
                     /translation="ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTR
                     RLVIADDDIEARLIGSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWY
                     INQSRTLSPTEREILRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLL
                     EAADILLCMRHCEASNVLHPY"
BASE COUNT          473 a          475 c          395 g          442 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactc
//
LOCUS       DAJTXQ010000281         1768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000281 DAJTXQ010000000
VERSION     DAJTXQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.287"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1517
                     /gene="tssI"
                     /locus_tag="OO515_004258"
     CDS             <1..1517
                     /gene="tssI"
                     /locus_tag="OO515_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6578011.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQ
                     TVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQV
                     GLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKD
                     GSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1593..>1768
                     /locus_tag="OO515_004259"
     CDS             1593..>1768
                     /locus_tag="OO515_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6578012.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVL"
BASE COUNT          452 a          425 c          559 g          332 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc
     1021 tggtggaaac ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg
     1081 tggggatcat tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta
     1141 tgaatacctc ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg
     1201 tgggaatggg ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga
     1261 tgaaggaaaa caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca
     1381 ttcatctgga aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg
     1441 gtgccacaca acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg
     1501 atatgcggca attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga
     1561 tgaaccatat taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc
     1621 agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag
     1681 gtgcgccgac gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg
     1741 tgaacccggt gctgggtgcg aaggtgct
//
LOCUS       DAJTXQ010000282         1755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000282 DAJTXQ010000000
VERSION     DAJTXQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.288"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..77)
                     /locus_tag="OO515_004260"
     CDS             complement(<1..77)
                     /locus_tag="OO515_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6578013.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKL"
     gene            338..673
                     /gene="iraM"
                     /locus_tag="OO515_004261"
     CDS             338..673
                     /gene="iraM"
                     /locus_tag="OO515_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6578014.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(954..1085)
                     /locus_tag="OO515_004262"
     CDS             complement(954..1085)
                     /locus_tag="OO515_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6578015.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1585..1661)
                     /locus_tag="OO515_004263"
     tRNA            complement(1585..1661)
                     /locus_tag="OO515_004263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1625..1627),aa:Arg,seq:tct)
BASE COUNT          584 a          430 c          296 g          445 t
ORIGIN      
        1 ttcagtttat gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca
       61 tagtgtttaa atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga
      121 catgtaatac agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga
      181 tatgcatgga taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa
      241 caacatcaga aaaataattc tgctctatgg tttacattca aaaatatcat ttatactttt
      301 cagaacatca ccagcaaggc ataaacaagg aaactaaatg aagtggattg tgattgatac
      361 agttatccag ccatcatgcg gaatatcttt ttcagtcata tggagtaaaa taaaattaat
      421 aatctggtat caatcggatg ctttcttacc tcctgaaagt atatttacac tgactcacac
      481 aggcatcatg ctcaataaca aagtgctacc tgtaaccatt tacaacgtag taccattcaa
      541 taaaacattc tggaattcaa tcaaaaatag ccaggaatgc cctacaaata cagataacgt
      601 attgaatgaa tgctttaata accgttgcac tctgcaaata tgtccttatg gactaaaaca
      661 acaaagtcca taaggagttt actcacatct gacaaaatca atataaacag cccctccgga
      721 gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc ccggtgaatt
      781 cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt gcccctccgc
      841 acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca gacaacagtc
      901 aaccgcctga attgtgaagt atttaaaaat ttctcccgct aactgatacc cggctaacag
      961 tctggcgttt tctttttcag caacgggaaa gcaacaacca ccacacccgc caccagcaca
     1021 ccgtcagcca gcactgacat tatccggctg ctgcaatgcc attcacaaaa acagtaagca
     1081 atcacttttt accgtaacag gtgataatcc agatatgtat ctaccccaga tgagtaatcc
     1141 gaagttcatc cataccacag gtcctggcta ttctgttgta ctcctgaaca agagcaaata
     1201 attctgaatt agcaaccatg aactcatcgc aaaccctctg tatagcatca ctattcagaa
     1261 taataacgtc tcttcccgaa agacgatcag gagtacagaa caaaactgtc aaacggctga
     1321 aggcctttgc tcgtgctgca ttgactatat caatacgctg cctaaggatg aaacaccccg
     1381 acgcctcatc aatattcact ctacccacac catatgaatg ataaatattt aatgctgaaa
     1441 aaaccattag accgtataac aaacactcaa tcaatactta acagaacttt tatttttgac
     1501 aaacataaaa tattttcaac aatatcctga gccaggtata tttcagtata aggctctgcc
     1561 ggaaggaatc tggaagaatg aatatggcgc gctgtactgg attcgaacca gtgaccgatt
     1621 gcttagaagg caattgctct gtccggctga gctaacaacg ctgaataccg ataatggacc
     1681 gccatcgggg acccgccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga
     1741 taagctaatg gcggt
//
LOCUS       DAJTXQ010000283         1749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000283 DAJTXQ010000000
VERSION     DAJTXQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.289"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            66..335
                     /locus_tag="OO515_004264"
                     /pseudo
     CDS             66..335
                     /locus_tag="OO515_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            450..620
                     /locus_tag="OO515_004265"
     CDS             450..620
                     /locus_tag="OO515_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578016.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(747..1304)
                     /locus_tag="OO515_004266"
     CDS             complement(747..1304)
                     /locus_tag="OO515_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6578017.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1301..1576)
                     /locus_tag="OO515_004267"
     CDS             complement(1301..1576)
                     /locus_tag="OO515_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6578018.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
BASE COUNT          494 a          360 c          424 g          471 t
ORIGIN      
        1 tttcaccata ttatagtttt atttgtgaat taaatcaatt atggcgatga attacaaggg
       61 gttaaatgct gccgcagcat agcgatattg aaatagccta gtatgcttcg atacagcagg
      121 agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt agtgagtata
      181 ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa agatcgttgc
      241 tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca actgcacctg
      301 atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc cgcagacacg
      361 tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt gttatttttg
      421 cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg gtgatgtcag
      481 caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat caccaggtaa
      541 agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat gaggttgatc
      601 gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat aacctgaatg
      661 atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa aattaacctc
      721 ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac caattgttct
      781 ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc cagagtgcat
      841 accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt atcgaagtgg
      901 atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg aggacgtttg
      961 tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag tctggctgct
     1021 tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat caggtagcat
     1081 cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc cgaaaagttg
     1141 gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac gtgtttatgt
     1201 tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc atctttgaaa
     1261 aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga tgactccaat
     1321 taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt acagggttgg
     1381 cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc cccaccataa
     1441 tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc gttgtgatgt
     1501 tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata tagccccgga
     1561 gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata aaattaaagc
     1621 gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat caggcagtta
     1681 aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt tcagatacaa
     1741 tgtcttatt
//
LOCUS       DAJTXQ010000284         1729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000284 DAJTXQ010000000
VERSION     DAJTXQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.290"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..1082)
                     /locus_tag="OO515_004268"
     CDS             complement(24..1082)
                     /locus_tag="OO515_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6578019.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1190..1430)
                     /locus_tag="OO515_004269"
                     /pseudo
     CDS             complement(1190..1430)
                     /locus_tag="OO515_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1655..>1729)
                     /locus_tag="OO515_004270"
     CDS             complement(1655..>1729)
                     /locus_tag="OO515_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6578020.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          412 a          507 c          379 g          431 t
ORIGIN      
        1 gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg tacttcccga
       61 accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg cccgagcgcc
      121 agtgccgctt tcactgtcga acccaacccc atgaaaaaat ctgcaatcag gtcacccgga
      181 cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca cggatgtttc
      241 ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac ctgcaccgtc
      301 acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata ctgccggttc
      361 agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc acgccgatgc
      421 ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct ttcattcggc
      481 aactgccact gaccggcact gaaccagtgc gacaccatgt ttttctttcc tgtggcatct
      541 gcaatctgtt ttgccgttat ccccagggca gctcgcgcat cacgaaagta agaaatcagc
      601 ggggccatca catgctgttt cagtgcactg cccttcgccg catacccggc atctttcgga
      661 cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc
      721 cgcaggcttt ccttgttgca tccgttccag cgtccggacg gcttcgccca gataatatgg
      781 ttcagcacac tgaagcgttc acgcatcatg atttcgatat cagatgccag gcgatgacca
      841 cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc cagacactgg
      901 tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc ctcaggcttc
      961 actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg taacgaccgg
     1021 ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac agtattaagc
     1081 atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag cagtgggcct
     1141 gaggtttgct tgtgaaccca acgcatgagc agatggctgg tgggtgcccc taacacccac
     1201 cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg
     1261 aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg gcagcgttcg
     1321 cgtgaaaggt aagtattctg cgctatctcc ccgactgtcg ccggttcggt aacgcttaat
     1381 tcattaaaca ccactctggc ggtttctgtc atatcctgct gttttagcat gtcttttcct
     1441 tttccggtta acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagaca
     1501 ggtattcacc gccaccagcg cgtttactgt actgacgcga tttcagtcat aaaaaacccg
     1561 ctcggcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca tcgttatgac
     1621 gaatttacca cagattccgg aaaaatcaac cttgttacct agttaccttt tttaactgcc
     1681 gctcagccca tgcttcttca atatcaaacc gggtcaccag cgcatcata
//
LOCUS       DAJTXQ010000285         1706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000285 DAJTXQ010000000
VERSION     DAJTXQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.291"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..153
                     /locus_tag="OO515_004271"
     CDS             <1..153
                     /locus_tag="OO515_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6578021.1"
                     /translation="AAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVL
                     AGSGLS"
     gene            323..601
                     /locus_tag="OO515_004272"
     CDS             323..601
                     /locus_tag="OO515_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6578022.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            601..978
                     /locus_tag="OO515_004273"
     CDS             601..978
                     /locus_tag="OO515_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578023.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1447..>1706
                     /locus_tag="OO515_004274"
     CDS             1447..>1706
                     /locus_tag="OO515_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6578024.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKF"
BASE COUNT          558 a          245 c          404 g          499 t
ORIGIN      
        1 gccgcagaat tattttcagg gatacggcac atcgctatta atattttaac gaatgataag
       61 gtattcaagg cagggttaag acgtaagatg cgaaaagcag ccatggatag aaactatctc
      121 gcgtcagtcc ttgcggggag cgggctttcg taatctttcc ctagggagtc agccgaatgt
      181 tgggaaggat ttgacggatg aagagaagaa ctgcttggcc ggcggcagta cgggaagccc
      241 gggaggatgg ggaccagaag atgagaatag cgcaagggat cgtatgcagc atgaacggta
      301 taaagatgaa ttgcgacaga aaatgggaaa acccgacgtt acagattcaa atttgaaaaa
      361 tattatagac gatctttatc ggccaaatgc aaaagtagga agtggaagca cagcagatgc
      421 tgttcgttat gaattggcca caggtgaaaa agttggtggt agaggacatg tattgaaagc
      481 acaggattat acaagggctt tacaggattg gttggataag aatccaacag catctccagg
      541 ggatcgagca gcagcagaaa acgttcttca ggatttacag aatgctttgg gaggcaagta
      601 atgaaccaat atattgctag ttatatagaa aaaatggcat cattttccac tgttttgtct
      661 gagacagtct cattagctaa tgaatactgg catccagaaa cgccaccggt ggttatgttg
      721 ttctctcagg ttggaaaaac gattgtaaaa aatttttatg aaatggggga tattgaaaga
      781 gaaaaaatat ttaaacatgt cgaagatggt atggtctcgc ttgatgatga attggccaca
      841 gttgtagcta cagggcttat agaggcaata gtcactgcga ccgatagcaa ttctttactt
      901 tggcaagatt taagtaaatt tctgggtgac aaatcaaagt ctcatgcatt agtgtggaaa
      961 aattttggtc aggagtgata ttattcaaga agtattaagc tcgctattga gatcttaatg
     1021 cttcttgaga ttaaattaaa gttaggacga agtaattgca ggtggaaata aatccatagt
     1081 gacgtgtggc aatggctatt agatgttatg gtgtgatggg ctttggtgat ttaagagcta
     1141 agttagattg ccttatgaaa aagtatggtt atatatttaa tgatgtgtat catatattta
     1201 aataaaaaac ggttctctct tgtcaagata gtaataggat agactattgt gttgataaat
     1261 ataattaaga gtctactttg ttgattttag aagactttgg agcagggaaa gatcatcgga
     1321 agtacacttt ttgtactaaa taattcgcgc taaatgttca ataattgaga tattccttat
     1381 tacctaaaac tatttttcat agcttatata tgatcaaata ctccttgcat aaataaggag
     1441 aacaaaatgg aacttaaaaa actgatggaa catatttcta ttatccccga ttacagacaa
     1501 gcctggaaag tagaacataa attgtcagat attctactgt tgactatttg tgccgtcatt
     1561 tctggtgcag agggttggga agatatagag gattttggtg aaacacatct cgattttttg
     1621 aagcaatatg gtgattttga aaatggtatt cctgttcacg atactattgc cagagttgta
     1681 tcctgtatca gtcctgcaaa atttca
//
LOCUS       DAJTXQ010000286         1677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000286 DAJTXQ010000000
VERSION     DAJTXQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.292"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..889)
                     /locus_tag="OO515_004275"
     CDS             complement(<1..889)
                     /locus_tag="OO515_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6578025.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="OO515_004276"
     CDS             complement(989..1345)
                     /locus_tag="OO515_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6578026.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="OO515_004277"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO515_004277"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          334 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactacc
//
LOCUS       DAJTXQ010000287         1673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000287 DAJTXQ010000000
VERSION     DAJTXQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.293"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..168
                     /locus_tag="OO515_004278"
     CDS             <1..168
                     /locus_tag="OO515_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HCR6578027.1"
                     /translation="FLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEP
                     DYEAALAVLKA"
     gene            complement(212..>1673)
                     /gene="tyrR"
                     /locus_tag="OO515_004279"
     CDS             complement(212..>1673)
                     /gene="tyrR"
                     /locus_tag="OO515_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6578028.1"
                     /translation="LRGIEIDPIGRIYLNFAELEFESFSSLMAEIRRIAGVTDVRTVP
                     WMPSEREHLALSALLEALPEPVLSVDMKSKVDMANPASCQLFGQKLDRLRNHTAAQLI
                     NGFNFLRWLESEPQDSHNEHVVINGQNFLMEITPVYLQDENDQHVLTGAVVMLRSTIR
                     MGRQLQNVAAQDVSAFSQIVAVSPKMKHVVEQAQKLAMLSAPLLITGDTGTGKDLFAY
                     ACHQASPRAGKPYLALNCASIPEDAVESELFGHAPEGKKGFFEQANGGSVLLDEIGEM
                     SPRMQAKLLRFLNDGTFRRVGEDHEVHVDVRVICVTQKNLVELVQKGMFREDLYYRLN
                     VLTLNLPPLRDCPQDIMPLTELFVARFADEQGVQRPKLAADLNTVLTRYAWPGNVRQL
                     KNAIYRALTQLDGYELRPQDILLPDYDAATVAVGEDAMEGSLDEITSRFERSVLTQLY
                     RNYPSTRKLAKRLGVSHTAIANKLREYGLSQKKNEE"
BASE COUNT          404 a          466 c          401 g          402 t
ORIGIN      
        1 tttctgcaag cttacggtgt ggcaattgct gatggcccac tgaaaggtct ggcagcgcgt
       61 gccgttgtgg ttattgacga aaatgacaat gtgattttca gccagctggt ggatgaaatc
      121 accaccgagc cggattacga agcagctctg gctgtactga aagcataagt taatatgcct
      181 gatggtgttg caccatcagg catattcgcg cttactcttc gttcttcttc tgactcagac
      241 catattcccg caacttattg gcaatcgcgg tatgtgaaac gccgagacgt tttgccagtt
      301 tgcgcgtgct gggataattg cgataaagct gggttaatac cgagcgttca aaacggctgg
      361 tgatttcgtc cagcgaacct tccatcgcat cttcgcccac ggctaccgtt gcggcgtcat
      421 aatccggcaa caaaatatcc tgtggacgca gctcataacc gtccagttgt gtcagtgcgc
      481 gatagatagc gttctttaac tgccgcacat ttcccggcca cgcataacgc gtaagtacag
      541 tattcaggtc agcggccagt ttcggacgct gcacgccctg ctcgtcggca aagcgggcga
      601 cgaacagctc agttaacggc atgatgtcct gcggacagtc acgtagcggc ggcagattga
      661 gcgtcaacac gttcagacga taatagagat cttcacggaa catgcctttt tgcaccagtt
      721 cgaccagatt cttctgcgta acgcaaatca cccgcacatc gacatgcacc tcatggtctt
      781 cgccaacccg acggaaagtg ccatcattaa ggaaacgcag taatttcgcc tgcatccgtg
      841 gtgacatttc ccctatttca tccaacagca ccgaaccacc gttcgcctgc tcaaagaatc
      901 ctttcttccc ttccggagca tgaccaaaca gttcactctc gaccgcatct tccggtatag
      961 acgcacagtt cagcgccagg taaggtttgc ccgctctggg gcttgcctga tggcaggcgt
     1021 aggcaaagag atctttacct gtacctgtgt cacccgtaat cagcagcggc gcgcttagca
     1081 tcgccagttt ctgcgcctgt tcgacaacat gcttcatttt cgggctgacg gcgacaattt
     1141 gactgaaggc gctgacgtcc tgggcggcga cattttgcaa ctggcggccc atacgaatcg
     1201 ttgatcgcaa catcaccacc gcaccggtca ggacgtgttg atcattttca tcctgaagat
     1261 aaacaggcgt aatctccatc aggaaattct gcccattaat aacgacatgc tcgttatgcg
     1321 aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa gccgttaatc aattgtgcgg
     1381 cggtatggtt gcgcaggcga tccaattttt gcccaaaaag ctgacagctc gccgggttcg
     1441 ccatatccac tttgcttttc atatcgacag agagcacagg ttcaggcaac gcctccagta
     1501 acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca cgggacagta cgcacatcgg
     1561 taacacccgc aatacggcgt atttcggcca tcagactgct gaaactctca aactccagtt
     1621 cagcaaaatt gaggtagatt cgcccaatgg gatcaatctc aataccgcgt aaa
//
LOCUS       DAJTXQ010000288         1654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000288 DAJTXQ010000000
VERSION     DAJTXQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.294"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..261)
                     /locus_tag="OO515_004280"
     CDS             complement(<1..261)
                     /locus_tag="OO515_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578029.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNK"
     gene            complement(511..666)
                     /gene="hokD"
                     /locus_tag="OO515_004281"
     CDS             complement(511..666)
                     /gene="hokD"
                     /locus_tag="OO515_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6578030.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(894..1559)
                     /locus_tag="OO515_004282"
     CDS             complement(894..1559)
                     /locus_tag="OO515_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6578031.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          428 a          431 c          363 g          432 t
ORIGIN      
        1 tttattcttc agcaaaagca gaaaaccagc cttaaaacca atctctttca aaacccgtaa
       61 tgatgtgaca aattcgtcct cacgcaaaat aaaaccgtcc gtcagaagtc cattacaaaa
      121 ataaaataac acagcaccgc tcttcctttg ttgagattgc aaacatctaa tgcggcaatg
      181 gctgacaatc gctccattct caacgcgcac agcatagagg ccattttcac taaaaatttc
      241 acgcaattct tcgattttca tcttcagaat ccttccagat aaatagctct cccctgttcg
      301 gggtccatcc ctcttctccc tgcgcgctac ttaagtgagt cgattctatc tgcgaaggtg
      361 cgcgaatcaa attcaccgga aataaacaac aaaaaacccg ccgaaacgct gagagatcac
      421 agcgatgggc gggttaagtg cgggtgcgtt gaggatgcct gacacatcag aggtgggcgg
      481 ggatgggatc agctccccgc ccggtcactc ttacttcctg gattcgtagt ctacgaagac
      541 agcgacctcc gtctggccgg ttcggattcg tacctcacag aggtctttcc tcgttaccag
      601 tgccgtcact atgacggtta aacagatgac gatcagggcg attaacatcg ccttttgctg
      661 cttcatagcc tgcttctcct tgcctttcgg catgtaagag gctaacctac atgtgcaaag
      721 catgaaattg gcctcagatt aatgttaagc gtcttgccgg acgcgtaatg ttaactgggg
      781 cttttctcta tctgcctttt ggtgttcatg cctgaagcag atagcctcaa gcacccgcag
      841 tcattctact tacctcgcca atatgaaatc aatcagaaag gtgccccata aaatcactcc
      901 ttctcttctt taccgtagtg gagttgacca attttgataa gagggcgtcc ctgagatttg
      961 cggtgtagat tggtatcgcg cagagaatac acacagccac aatattcctg ctgatagaat
     1021 ttttcgcgct tgctgatttc aatcatacgg gacgagccgc cctgcttgcg ccagttataa
     1081 tcccagtaca ccatgcccgg ataatgcgcg acggctcgcc gcccacagtc gttaacctgc
     1141 tgcatatttt tccagcgtga aatgcccagt gaactgctga tcacactgaa accattttca
     1201 gcggcgtaca gcgctgtccg ctcaaaacgc atgtcaaaac acatggtgca acggatccct
     1261 cgttcgggct cccattccat tcctttggct cgttcaaacc agttgtctgt gtcgtaatca
     1321 gcatcaataa acggcacgcc gtgttgttca gcaaagcgaa tattctcatc cttacgaatt
     1381 aaatactctt tctgaggatg aatgttcggg ttgtagaaaa agatggtgta gtcgattccc
     1441 gaggcctgaa gcgcctccat cacctcaccg gaacatggag cacagcaaga gtgcagtagt
     1501 agtttgtttg ccccgtttgg gagctccaat ttgggccgtt tgaaatcagc aactgtcata
     1561 aatatgttta ttggggtcat aaaaatatca aagagtgtag cattagaacg gggctatcgg
     1621 aaacagatgt gtgactgctc cccgcccttt cggg
//
LOCUS       DAJTXQ010000289         1638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000289 DAJTXQ010000000
VERSION     DAJTXQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.295"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>1638)
                     /locus_tag="OO515_004283"
     CDS             complement(<1..>1638)
                     /locus_tag="OO515_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6578032.1"
                     /translation="PDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDRQGRLAEETSRN
                     GNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTDCSGYTTRYEYDRYGQQ
                     IAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTAIVAPDGSRSEIQYD
                     AWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQR
                     YQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGWLTEISHLSE
                     GHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSEQGLATRQEPDGLPPV
                     EWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAWNTSGQLRSRHL
                     NLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPA
                     GNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDERT
                     HHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          242 a          551 c          470 g          375 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgaggtctc ttccgccagc ctgccctgac ggtcatattt ccggctgctg
     1561 cgtaaaccat ccggatatgt cactgacgtc agttgcaact ggttgttgta accataccgt
     1621 accgtcctgc cgtccggc
//
LOCUS       DAJTXQ010000290         1620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000290 DAJTXQ010000000
VERSION     DAJTXQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.296"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            123..953
                     /locus_tag="OO515_004284"
     CDS             123..953
                     /locus_tag="OO515_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCR6578033.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1094..>1620)
                     /locus_tag="OO515_004285"
     CDS             complement(1094..>1620)
                     /locus_tag="OO515_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6578034.1"
                     /translation="SPDANVCNDAGPFELLQARQLLESNIAEFAALQATREDIVKMRQ
                     ALQLEERELASSAPGSSESGDMQFHLAIAEATHNSMLVELFRQSWQWRENNPMWIQLH
                     SHLDDSLYRKEWLGDHKQILAALIKKDARAAKLAMWRHLENVKQRLLEFSNVDDIYFD
                     GYLFDSWPLDKVDA"
BASE COUNT          488 a          361 c          329 g          442 t
ORIGIN      
        1 cgtgtagagt tgatatcact acaacagaaa catgcatgtc atttgtttac ttctcttttt
       61 actacagaaa aatttatcta tttttctaaa catcagtgaa atactcataa aaggtaacta
      121 ttatgtctat gccattaagc aatgcccttc agagtcaaat tattacagat aatcacttcc
      181 ttcatcaccc aatagtcgac agtgagttta cacataaatt aaaatatgca cgcatggatt
      241 tggaaaacat ctatcttccg cctcttactc gtggcaataa ccataattac gatggaaaaa
      301 gcgctgtcga aattagaaaa cttgatatta gtaaagagtc ctggccattt aattatgtga
      361 caggagcatg cagagaatcc gatggtatta caacaacagg acgtatgctc tatcggaact
      421 tgaaaatcac ctcagcttta gacgaaatat atggtggcat atgtaaaaag gcgcatgctg
      481 ccacagagtt agcagaaggt ttacggctaa atttatttat gaagagccct ttcgatcctg
      541 ttgaggacta tacagttcat gaaataacat taggccctgg ttgcaatgtt cctggttatg
      601 ccggaaccac gataggatat atttcaaccc ttccagcttc tcaggcaaaa agatggacca
      661 atgagcagcc aaggattgat atctatattg atcagataat gacggtaaca ggggtagcaa
      721 attcttccgg attcgccctt gccgcattac ttaatgctaa tattgagttg ggtaatgacc
      781 caataattgg tatagaagcc tatcccggta ccgcggagat ccatgcaaaa atgggatata
      841 aagttatccc gggtgatgaa aatgcaccac tcaaacgtat gactctgcaa ccgtcatctc
      901 ttccagaact gtttgaatta aaaaatggtg agtggaatta tattggtaaa taactaccag
      961 gaagaatgag tactaacatt caggttatac agatagctaa acaataccct gcaatttaca
     1021 aagtaatact cattgcgact tcttaattta agaaagcgca aggaacattt acccttgcgc
     1081 ttattataat aagtcaggcg tcaactttat ccagcggcca tgaatcaaac agatagccat
     1141 caaaataaat atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat
     1201 gccgccacat tgccagcttc gccgctcggg catctttttt gattaacgcg gcgaggatct
     1261 gtttgtgatc gcccaaccac tctttgcgat acaggctgtc atccagatgg ctgtgcaact
     1321 ggatccacat tggattgttt tcccgccact gccaggactg acggaacagc tccaccagca
     1381 tgctgttatg cgttgcttcg gcaatggcga gatggaactg catatcacca ctttcgctgc
     1441 tgcccggcgc gctggaagcc agctcacgct cttccagttg caatgcctga cgcattttga
     1501 cgatatcttc gcgggtagcc tgcaaagcgg caaattccgc gatgttactt tccagtaact
     1561 gacgcgcctg taacagctca aaaggaccgg catcattgca gacgttagca tccggactgt
//
LOCUS       DAJTXQ010000291         1595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000291 DAJTXQ010000000
VERSION     DAJTXQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.297"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..828)
                     /locus_tag="OO515_004286"
     CDS             complement(<1..828)
                     /locus_tag="OO515_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCR6578035.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNDDGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     "
     gene            1203..>1595
                     /gene="garP"
                     /locus_tag="OO515_004287"
     CDS             1203..>1595
                     /gene="garP"
                     /locus_tag="OO515_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6578036.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFP"
BASE COUNT          396 a          384 c          362 g          453 t
ORIGIN      
        1 ggtttctcgc tgccgttgat tcagtttttg tagatggcgt tcggcgacct gcaaaatatc
       61 ctcgaccatg gactgaaagc cgacatgctt ttcatcctgc aaactgacaa tgctggcgct
      121 ttctactgga atagcttgca catcatccgt gccgaccagc aggcgctcag gctgcaactt
      181 ttcacaaccc agaccaatca ccattacttc gccgccaaag ttagggttca gcgaaatatt
      241 gtggatggta cgaataggca caactgccgc cggtgcgtta atcgccacgc cacagccgta
      301 caaatgattc agccccacca cgccatcgac gttcgggtat ttcggtagca gatcgcgttc
      361 aatgattttt actacatagt ccaccacgcc tgccacacag tggacgctgg tggtgatgcc
      421 gagcaggttt ttggtgccca cgctgccatc gtcattgcga tagccctcaa aggtgtatcc
      481 ttccagcggc ggtaaggggg ccgggacttt ggttgccagt ggcagcgtgt gtaacggcgg
      541 cgcttccggt agtacaacca ttgattcgtc gatccagctt ccgcgtggga ttgcgcgcac
      601 ggcgtaacca atcacttcgc cataacgaat aatttcacca ttagctggaa tgtccagcaa
      661 tgcgacttta tgcccctggg gagtatgttc aattaattcc agcccatccg gaaaacgcgt
      721 tcctgctttc aggccattat cattaacaat aattgccaca ttatctgtgt cgtgaacttt
      781 tatataaaac gcagttggcg tttcttgtct gatttcgatg ttggccattg tgcaatattc
      841 tccagccagg ggttattaaa atgaggatct ggtctcattt aaaaataaat gacttcattg
      901 ggtgaaataa gaatgtcagg agtttaaacg cagaaatgtg atcaatgtca atcatttcag
      961 ggattgcagt gccgcccggt aaggtaaagc gcaaatctgt gctttagcgc gcaaccttat
     1021 gtgcatatgc tcaaaataat atttaaatag tactgattaa attagcattt gcacaatgat
     1081 tattattctc ccgctgctta tactaattcc cgagttcatt gtcgtaacgg cataaataaa
     1141 actcataaat aacaccttcc ccataataat aaaaatcagc ataagtaccc gaggtaaata
     1201 aaatgattct ggacaccgtt gacgtaaaaa agaaaggcgt gcataccagc tatttaatat
     1261 tactgattat ttttattgtt accgccgtta actacgccga tcgtgcaacg ctgtctattg
     1321 ctggtaccga agtggcaaaa gagttgcagt taagtgcggt ttcgatgggt tacatcttct
     1381 ctgcttttgg ctgggcctac ttgctgatgc aaatccccgg cggctggttg cttgataagt
     1441 ttggctcgaa aaaagtttac acctacagcc tctttttctg gtcgctgttc accttcctgc
     1501 aaggctttgt tgatatgttc ccgctggcct gggcagggat ctccatgttc tttatgcgct
     1561 ttatgctggg gttctcggaa gcgccatcat tcccg
//
LOCUS       DAJTXQ010000292         1578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000292 DAJTXQ010000000
VERSION     DAJTXQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.298"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..64)
                     /locus_tag="OO515_004288"
     CDS             complement(<1..64)
                     /locus_tag="OO515_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA-like transporter family protein"
                     /protein_id="HCR6578037.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQN"
     gene            complement(61..579)
                     /gene="mddA"
                     /locus_tag="OO515_004289"
     CDS             complement(61..579)
                     /gene="mddA"
                     /locus_tag="OO515_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6578038.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            760..>1578
                     /gene="curA"
                     /locus_tag="OO515_004290"
     CDS             760..>1578
                     /gene="curA"
                     /locus_tag="OO515_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6578039.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVL"
BASE COUNT          380 a          386 c          439 g          373 t
ORIGIN      
        1 tgttctggac taccagacca attccggcgg caatcaaaaa agcaagggtg agcgactgat
       61 tcatccaatc gcgtccggtt cagtgcgttc gtcgagttgc aactgcataa atgtcagatc
      121 cagccagcgc ccaaatttag tgcccacctg cggcatttgt gcggtgacga caaatcccag
      181 cgaatggtgc agatgcagcg aggcctgatt ttgcgattcg atcccggcga ccatgacatg
      241 cttcccgcaa tcccgcgctt catcaatcaa tcggcttaac aatttacgcc ccagaccttt
      301 gccctgatga tcgggatgga cataaaccga atgttccacg gtatggcgaa aaccatcgaa
      361 actacgccag tcgccaaacg aggcatatcc cgtcactacg ccatcttctt cacttaccag
      421 caccggataa cctgctatgg tccgcgcttc aaaccaggca atgcggttat ctgcatcaac
      481 cgtttggtca ttccagatag ccgccgtata caacacggcg tggttataaa tttccgcaat
      541 ggcagcacaa tcggctttgc gggcaaaacg gatggacatg tctggcctcg ataaaataat
      601 gatgatgatg ttatcaatac tatcgaaaac agcccgaagc aacggattcc tcgatgaaat
      661 tattgcgtgt tgcagagggg atgtgacggc tgcaaacaaa accaatcccg tgagcctgct
      721 actattgatg gattcatgcc aaaagcacag gagagcatta tggggcaaca aaagcagcgt
      781 aatcgacgtt gggttctggc ctcgcgtcca catggcgcac cggttccgga gaatttccgt
      841 cttgaagaag atgatgtcgc cacaccgggt gaaggacagg tgttactgcg cacagtttat
      901 ttgtccctgg acccgtatat gcgtggacgt atgagcgatg agccatctta ttcaccgcct
      961 gttgatattg gcggcgtgat ggtcggcggc acggtgagcc gtgttgtgga gtcgaatcat
     1021 cctgattatc agcctagcga ctgggtgctg ggctacagtg gatggcagga ctatgacata
     1081 tccagtggtg atgatctggt gaaacttggc gatcatccgc aaaatccatc gtggtcgctg
     1141 ggtgtgctgg ggatgccagg ctttaccgct tatatggggc tgctggatat cggtcagcct
     1201 aaagagggcg aaacgttggt ggtagctgcg gcgacaggtc ctgtgggagc aacggtgggg
     1261 caaatcggca aacttaaagg ttgcagggtg gtgggggttg ccggtggcgc ggaaaaatgc
     1321 cgccatgcta tcgaggtgct gggcttcgat gtttgtcttg atcaccacgc ggatgatttt
     1381 gccgaacaac tggtgaaagc gtgcccaaaa ggtattgata tctattatga aaacgtgggc
     1441 ggtaaggtat ttgatgcagt gctaccatta cttaatacat ctgcgcgcat tcccgtttgt
     1501 ggcttagtga gcagctataa cgctacagag ctaccacccg gtccggatcg tttacccctg
     1561 ttgatggcaa cggtgctg
//
LOCUS       DAJTXQ010000293         1565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000293 DAJTXQ010000000
VERSION     DAJTXQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.299"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="OO515_004291"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="OO515_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR6578040.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..>1565
                     /gene="hipA"
                     /locus_tag="OO515_004292"
     CDS             641..>1565
                     /gene="hipA"
                     /locus_tag="OO515_004292"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCR6578041.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQSNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGAT"
BASE COUNT          437 a          340 c          415 g          373 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagtcc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacgg
//
LOCUS       DAJTXQ010000294         1560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000294 DAJTXQ010000000
VERSION     DAJTXQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.300"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>1560)
                     /locus_tag="OO515_004293"
     CDS             complement(<1..>1560)
                     /locus_tag="OO515_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6578042.1"
                     /translation="PSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFG
                     PWRLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQ
                     LASDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSN
                     SGDLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAE
                     ATAIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFH
                     GYQWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQ
                     TIFDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRAL
                     SLNLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNN
                     HNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALID
                     ANGASGVRIQN"
BASE COUNT          386 a          357 c          386 g          431 t
ORIGIN      
        1 agttttgtat cctcacgcca gaagccccat tagcatcgat aagcgcaaaa gcgttgccca
       61 gatattgcga gagcgtcacg ccatgagtat ggataaccac gccgccggta acgccgtaat
      121 tgtattggct gctgtcatta ccgtagctgt atccggcact gaaggtgcca taaggtgaac
      181 ggtaactggc gttcaggcta ctgttatggt tgttatcgtc atccagactt tgttccaggc
      241 tatagctcag gcgaccatca tccagcagtg agccattaag acgcatttca tgttgggttg
      301 ggcgatcttt ctggctggtc atctgatagg aaacccggct acgcggtaac cagcgttcta
      361 acggaatact gagattcaaa gagagtgcgc ggtcattatt ttgatccgtg tagcgactgt
      421 attgataatt caggctgtaa ccaattcccc attgctgacc ggaaacccca actgagatat
      481 tcctgttttt atcgttattg ccccaatagt cttgctgcga accggaggca tacagactta
      541 ccccatcaaa tattgtctgg ctgatgttga attgaatttc acttttttgg cgactgttat
      601 agttccaatt acgggtattg gcttcatcaa aactaaaata gccatcgttg gtatagcgat
      661 agtagctgac agcgatattg gtgttggttt ccgggatatc tttgatgtac tgcgtacgcc
      721 attgatagcc atgaaaagag tgctggttat cgaattgggt gtcagctctg ttgatatcca
      781 tcgacaacgc gcccagtgcg ccaagtgtgc cgccgatacc gatccccagc gcataataat
      841 cttcagaacc gagcaggcca ccatacagcg taaaagtatt attcaaacca tatattgccg
      901 tggcttcagc aaattcgggt tctttgctat cactgtttgc atcagcgcga tagcgtccag
      961 cggtcgcgct atattttaga tgcccaggtc gctgcatcat gggtaatgaa gaataaggct
     1021 ggataaagcg acgttgcgta ccgtcacttt cttcaatcgt gacttctaaa tcgccgctgt
     1081 tggaagaggg gtagagatcg ttaatttcaa aggcacccgc tggcacgttg ctttgataga
     1141 tcacataacc attttgcctg atagtcacga ttgcactact gtttgcgata ccgcgtaccg
     1201 ttggggcaaa tccgcgctgg ctgtttggca acatattatc gtcggaagcg agttgaacgc
     1261 cagtaaaggt gtagctggaa aaaatactgc cgctggtggc gctttctccc agaagcaact
     1321 gagacttcaa tgccttgata tcacgttgta aataactact gatggtattc cagcttgatg
     1381 tctgatcgtt gcgtgtccat gtggaatagt tgcgtaatcg ccaggggcca aaattagcac
     1441 cattttgcat attcaggtac tgtcgttggc tacgattgcc ctggcggtaa cggttatcag
     1501 aacctgtaaa cgagtagttg gtaaacagcg ttggtatacc atcgtcccaa cgagaagggg
//
LOCUS       DAJTXQ010000295         1545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000295 DAJTXQ010000000
VERSION     DAJTXQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.301"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(774..1445)
                     /gene="queE"
                     /locus_tag="OO515_004294"
     CDS             complement(774..1445)
                     /gene="queE"
                     /locus_tag="OO515_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6578043.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          494 a          326 c          283 g          442 t
ORIGIN      
        1 atcaattttc acttaaagta tgaactggat atctttattc ataagaacta ttcatcaaca
       61 gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac aaatttccag
      121 atttattact taatcaatga ataacaagct taaaaaacaa actattaacc taaagaaaaa
      181 attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt aacgatttaa
      241 gaatcataca aataacactt tgattaaatt taattttttg tatcgataat tgtgaacctc
      301 tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc ttattacaag
      361 tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt tatccccgct
      421 ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta agccagtcac
      481 aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa gagtacttaa
      541 ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa tctaccaaac
      601 tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg tagagaatgc
      661 taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa acaatataaa
      721 tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt taatcaggca
      781 atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat gcaggtttca
      841 atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat gactcgcggt
      901 ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg tacgcgcccc
      961 actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc atagccgccg
     1021 cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg tacctcatga
     1081 gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc agtcagtggt
     1141 agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc ggtgtatccc
     1201 tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt atcactctct
     1261 ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt ttcccaggtg
     1321 tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat aaaaatggcg
     1381 gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt aatcgggtac
     1441 tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg ccacaacaat
     1501 catgtcttat taacattctg ttacaggcag atttaaagaa aaatg
//
LOCUS       DAJTXQ010000296         1545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000296 DAJTXQ010000000
VERSION     DAJTXQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.302"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..571)
                     /gene="ghxP"
                     /locus_tag="OO515_004295"
                     /gene_synonym="yjcD"
     CDS             complement(<1..571)
                     /gene="ghxP"
                     /locus_tag="OO515_004295"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6578044.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVG"
     gene            complement(1117..1533)
                     /gene="soxR"
                     /locus_tag="OO515_004296"
     CDS             complement(1117..1533)
                     /gene="soxR"
                     /locus_tag="OO515_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6578045.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
BASE COUNT          432 a          433 c          366 g          314 t
ORIGIN      
        1 gacctaccag tgacataatc accgggaagg tcgcgaaatc acccagcgca acgggcagac
       61 catcaagcgg gtttttaatc accagaccga caccattagc ggcaatgagc agcaggaaca
      121 gaccgatacc gatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
      181 tacggatacc cgtggcagaa attaccgtaa acagcacacc catcaggaac acggcaccca
      241 gcgcgacagg tacgctaata tgttgcccca gcaccaggct gaatgcggta aacgccgtca
      301 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
      361 cgagtccggc aaccagacag gttgcaacga aaactgccgc aggcgggaag cctgctttac
      421 ccaacatacc tggaacgacg atgaccgagt agaccatcgc cagaaacgtt gttaacccgg
      481 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
      541 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
      601 cgtcaagatg cgtggcggac aatcccctgc cagagaaacg ttatcttcct gctccaggtt
      661 tgcgacaatt tactgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
      721 ggtaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
      781 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
      841 cgtcggtgcg caagattgca gcgttatcga gccgtatacc gttcaggatt gcgataaaaa
      901 atcgtctgag atgacaaaaa tattgcggat aaaaagtata cttttacact ggcgaagaaa
      961 tgccggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
     1021 ggggtgtgtt gacgtcgggg gaaaccctcc tgtgtaccag cgggatagag agaaagacaa
     1081 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgttcat cttccagcaa
     1141 gcgtgcgccg gtaccttctt ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact
     1201 gcgcgaaagg cagccacaac caatacatcc gtccagttcg tcacgcagcg ccactaaggt
     1261 atgaatgcgc cgatccaact cttctcgcca ttgggacgaa agctgtttcc actctttcgc
     1321 acttaacgta tgcccttcgg gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat
     1381 gccaatacgc tgagcaattt tgataattgc aacatatcgc aacacatcac gtttatatcg
     1441 ccgctgattg ccgctgttac ggatactggt aatcaaccct ttactttcat agaaatgcag
     1501 cgccgatacc gccacaccgc tgcgtttcgc cacttcgccg ggggt
//
LOCUS       DAJTXQ010000297         1514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000297 DAJTXQ010000000
VERSION     DAJTXQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.303"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..317
                     /locus_tag="OO515_004297"
     CDS             <1..317
                     /locus_tag="OO515_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578046.1"
                     /translation="ASKKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGANGA
                     IRELEKFRQQGFTPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGF
                     RG"
     gene            324..1070
                     /locus_tag="OO515_004298"
     CDS             324..1070
                     /locus_tag="OO515_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6578047.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1085..1507
                     /locus_tag="OO515_004299"
     CDS             1085..1507
                     /locus_tag="OO515_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6578048.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          379 a          348 c          467 g          320 t
ORIGIN      
        1 gagcgtctaa aaaattcgag ccggaggcta ttgagctgcc cgattggttg ccggaaacac
       61 tctggcatga gtgggtccgg ttcagacagg cattgcgaaa accgattcga acggagcagg
      121 gcgctaacgg ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc
      181 aggtgattcg acacagcatc gccaatgaat accagggcct gttcgcgccg aaaggtgttc
      241 ggcctgagac gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc
      301 caggcttcag ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca
      361 tccgccgact gaccccgcca catgtaaccg ccccattcag aacggtagcg gagtggcgcg
      421 agtggcaact tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt
      481 tgcgggtgga aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt
      541 ttgcgaatta ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca
      601 tcgccgatga actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctggtaccg
      661 ggaagaatca cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga
      721 ttgtggttac cgtggctgat gttatgagcg ccctgcacgc cagctatgac gacgggcagt
      781 caggcgaaaa atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg
      841 gcattcagcg cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga
      901 cagcgtcgat gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat
      961 tgctcggcga gcgggtgatg gatcgcatgg tcatgaacgg cgggcgctgg gtgaatttta
     1021 actgggagag ctggcgtccg aatgttagcc attcgagggt tgttaagtag tttcaggagg
     1081 atttatggcg aaacctttta ctcccgaaca gcgggaagaa ctgaagacgc gaattgtgga
     1141 actcgtgcat caggacggtc gggtcacgat tcggcagttg tcagatgaaa caggtatcag
     1201 tcgtgcgtct gtcggtcgct tatgcataga actggtcgca agtggtgatg tatataattc
     1261 tggctacggc ttattcccgt ctgaacaggc tcgcaaggac tggcaaagcg cccgcaaaaa
     1321 actctcgaga gtaaaggtga ggaaaccggt tgttgttgat ccggacctta tctggtcatt
     1381 acctgacgga gaaatacgcc gctacgacag gcgcctgaat ataatctgtc gcgagtgccg
     1441 gaagagcgaa gctatgcagc gtgtactggc tttctatcag ggtaattttc aggaggcgat
     1501 actgtgaatg aaat
//
LOCUS       DAJTXQ010000298         1460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000298 DAJTXQ010000000
VERSION     DAJTXQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.304"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            607..>1460
                     /locus_tag="OO515_004300"
     CDS             607..>1460
                     /locus_tag="OO515_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6578049.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLV"
BASE COUNT          355 a          314 c          405 g          386 t
ORIGIN      
        1 cacagacacg gattaaaatc cgcatccgtt caccgttttt taaagtccgt taaaagcatg
       61 atgccatctc cgagagttaa tctcgtcaaa tgctaaatcg tgggggtccc ctttggggtt
      121 ccgatttagt gattgacgac accaccgatt aaaaaactta tgcggggtgg atggtttcac
      181 gaagtgaggc catccacctg taagacaggg ttttgttttt attccctgtt ttggtgatcg
      241 ggtgtgtgga aaaggttggg gtaagccgtt cgggggtgct tgttttgggg ggttaaaatt
      301 gtggttattt tttgcgcaat tctcgcgcgt gatccttgta tttatactta agggataaat
      361 ggcggatatg aaatagtggt ttagcccagt aatgacgagg ctttgagtgg gttttgacag
      421 gtcaaagaaa atggagcaga attgaggcgt ttttaatcgg cgttggggag tgcgtcaaca
      481 ctccccaaca tttcgaatgt gtcacctcag cggcaaactc tggtgacatg tactggctcg
      541 caatgcacag gtacgtgatg aatataccac atcaaatcac agcctgccca gatcggagca
      601 ggcttaatgt cagaagataa attcctttcg gactacagcc cccgtgatgc agtttgggat
      661 acccagcgca cgcttaccga ttctgtcggg ggtatctacc agactgctgc tgaattcgag
      721 cgctatgcac tccgtatggc ctcctgtagc ggtttgttac gttttggttg gtctaccatc
      781 atggaaaccg gagaaacgcg cctacggctt cgtagtgcgc aattttgccg tgtccgtcat
      841 tgccctgtct gccagtggag aagaaccctc atgtggcaag cccgttttta tcaggctcta
      901 ccgaaaatcg ttgtggatta cccgtcttcc cgatggttgt ttctgacgtt aactgtcagg
      961 aactgcgaga taggtgaact tggaacagtc cttacagcaa tgaatgcggc gtttaagcga
     1021 atggaaaagc gaaaggagct atcacctgtt caggggtgga tcagggctac ggaggtgacg
     1081 cgaggtaagg atggcagcgc acatccgcat tttcactgtc tgctgatggt gcaaccttct
     1141 tggtttaaag ggaagaacta cgttaagcac gaacgttggg tagaactctg gcgcgattgc
     1201 ttgcgggtga actatgagcc gaatatcgat attcgggcag taaaaactaa gacaggtgag
     1261 gttgtggcca acgttgccga gcaactgcaa agcgcggttg ctgaaacgct gaaatactcc
     1321 gttaaaccgg aagatatggc aaacgatcct gagtggtttc ttgagctgac gcggcagctt
     1381 cacaagcgcc gttttatctc gaccggtggg gcgctaaaaa acgtcctcca gttggatcga
     1441 gaaaccaatg aggatcttgt
//
LOCUS       DAJTXQ010000299         1440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000299 DAJTXQ010000000
VERSION     DAJTXQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.305"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            154..1269
                     /locus_tag="OO515_004301"
                     /pseudo
     CDS             154..1269
                     /locus_tag="OO515_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          570 a          257 c          211 g          402 t
ORIGIN      
        1 tattaccggg tggagataaa agggaaatca aaggtaatta tatcaggaag atataacgca
       61 ttacatttat tgtgtgtaga agaaaatcca ttcctgttct aattatccaa tttaaacatc
      121 ttagcataaa acaaatgatg aataaggaat tttatgtcaa ttgactttac cccaggtata
      181 ataaatacat atcacggcga tatttataac tgcacaacaa ataccgataa tgctaaaacg
      241 ccagacacac cgaagtggcc ttgcgataat tgggaagaac aacaacccat taattccaca
      301 ttctctggag aaggatatat ctctgatcaa tatgatttag tgcaacacca acttcaacaa
      361 attaatgcgt gccacacgaa cactacatat acaaatgcag actactcaaa agttgtggct
      421 caacttgtaa gtcttattac taacattgaa acgataagtt cgacacaact tacgcaacag
      481 acacaatcaa tacttaatca gattaataac attagatatg aaaaaaacaa aagtgctgaa
      541 tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg caatcataac taaaattagc
      601 gtagaagagg gaatacccat aacattcagt gtccaaacca tgttttcaga tactaatttt
      661 attgctgaac aacgagctga cttaccaact aatataaaag atattcaaag cctgtatcaa
      721 aaaatgacga agttatatat tgagcacagt gaaaataaaa acaggatgaa agtctttgcc
      781 ggagccaact ttattgattt caatatgaca ggacagaacc tgtccgggtt cgttctaacc
      841 ttgtcaaggt tttatttcga agatctactt aatataaatt tcactgacgc aaatttaggt
      901 gacactattt tttacataaa gaacacccta cccccaaatt atataaagat ggacaatatc
      961 ttgacaaaca aatcgaaggt ttattttcaa cattattaac cattaatgac aactttttac
     1021 gagcaaaagc ggaaattgct tcgacaataa ttaaattttt agaggccagg ataaccaatc
     1081 tttcatacaa tgacatatta aaatatcagc aagaattcca aaaacaatgt tataagcaag
     1141 ttaaagcctt tacgacactc tctcgataca ataaaattca aacatgggca gaaatgtctg
     1201 aatatcagtt cgaagtattt caatacgaga cgcttaaccc caaaaaaatg tcacacacgc
     1261 cttatttaaa gaggccactt cctaatgaaa aagatataaa ttatggggtg gaaatagaaa
     1321 taccatctgg caaaagaatt cgcttatcaa atcattatca gaatataata ccataatatt
     1381 gattattagc actttataat tattgaatga aaatagattt tatgtaattt taaaacaaag
//
LOCUS       DAJTXQ010000300         1436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000300 DAJTXQ010000000
VERSION     DAJTXQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.306"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            89..>1436
                     /gene="istA"
                     /locus_tag="OO515_004302"
     CDS             89..>1436
                     /gene="istA"
                     /locus_tag="OO515_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCR6578050.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKY"
BASE COUNT          367 a          401 c          364 g          304 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttgaatcc atgctgaaag ccaaacccca
     1381 tccggagctg gcttaccgtg cagtcctggg tctgctggca cttcagaaaa aatacg
//
LOCUS       DAJTXQ010000301         1426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000301 DAJTXQ010000000
VERSION     DAJTXQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.307"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..118)
                     /locus_tag="OO515_004303"
     CDS             complement(<1..118)
                     /locus_tag="OO515_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6578051.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO515_004304"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO515_004304"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6578052.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="OO515_004305"
     CDS             complement(822..>1426)
                     /locus_tag="OO515_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6578053.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAJTXQ010000302         1411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000302 DAJTXQ010000000
VERSION     DAJTXQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.308"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1334)
                     /locus_tag="OO515_004306"
     CDS             complement(<1..1334)
                     /locus_tag="OO515_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6578054.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          413 a          397 c          341 g          260 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcaa agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg g
//
LOCUS       DAJTXQ010000303         1403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000303 DAJTXQ010000000
VERSION     DAJTXQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.309"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            204..1400
                     /locus_tag="OO515_004307"
     CDS             204..1400
                     /locus_tag="OO515_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6578055.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
BASE COUNT          295 a          436 c          377 g          295 t
ORIGIN      
        1 atggggtgtt agccagccag acccggcatg attactgccc ccagtcgtcc atgatccggg
       61 gggtgatgtc accgggtctg gtggggcgct ggtaaccgct aataggggtc aggtcaggca
      121 cttttgccgg gaccgtctgt aacgtggatg ccggtacctg ctccccgtgg ttatctggtt
      181 aacccatata caagggagac agaatgaccg aatccagcga ttacgaatcc gtccaggtct
      241 ttatcggcgt tgatgtcggt aaagatacgc atcacgctgt tgccattaat cgttcaggta
      301 aacgcctgtt cgataaagca ttacccaacg acgaaaacaa actcaggtcg ctaatatctg
      361 acctgaaaca acatggtcag atactgctgg ttgttgatca gccagctacc atcggtgcgt
      421 tacctgtcgc cgttgcccgc tcagaaggag tccttgtcgg atacctccct ggactggcca
      481 tgcgccgcat agccgactta cacgccggtg aagctaaaac tgatgctcgt gacgctgcca
      541 tcattgccga agctgcccgt accctgcctc acgcgctacg cacgctgaaa ctggctgacg
      601 agcaaatcgc cgaactctcc atgctctgcg gcttcgatga tgatcttgcc gcacagacaa
      661 cgcaggccag caaccgtatc cgcggccttc tgacccagat acatccggca ctggagcgcg
      721 ttctcggtcc gagacttgag cacccggcgg tactcgatct tctccagcga tatccctcac
      781 cagaaaaact cgcttcgctg ggtgagaaga agctggcagc ccagctctgc aaacttgcgc
      841 ctcgtctggg taaacgcctt gcagcagaca tagctcaggc actggccgaa caaaccgtcg
      901 tcgttcccgg cacgaatgcc gctgccgtag tactgccacg tctggcactc cagctcatca
      961 cgctgcgtaa gcaaagagac gaggtggcgc ttgaggtaga acagcgagtt cttgctcacc
     1021 ctctttaccc ggtcctgacc agtatgcccg gagtcggtgt caggaccgca gccagactcc
     1081 tcaccgaggt cgcctgccgc gccttcgcct ctgccgcaca tctcgctgct tatgctggcc
     1141 ttgcgccggt aactcggcga tccggctcgt caatacgcgg tgagcatccc tcgcgacggg
     1201 gtaataaagc tctcaaacgg gcgttgttcc tgtcggcctt cgccgcgctc agggatccgc
     1261 tctccagggc ttactacacc cgcaaaatga gtcagggaaa acgacacaat caggcgctta
     1321 tcgccctggc gagacgacgc tgcgacgttc tgttcgccat gatgcgcgac gggacttttt
     1381 ataccccgca ggggtcataa cat
//
LOCUS       DAJTXQ010000304         1384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000304 DAJTXQ010000000
VERSION     DAJTXQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.310"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1139
                     /locus_tag="OO515_004308"
     CDS             <1..1139
                     /locus_tag="OO515_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6578056.1"
                     /translation="PLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGA
                     VMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERD
                     AQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSF
                     GLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQE
                     KTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGR
                     IASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDI
                     PAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR"
     gene            1158..>1384
                     /locus_tag="OO515_004309"
     CDS             1158..>1384
                     /locus_tag="OO515_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR6578057.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMG"
BASE COUNT          298 a          344 c          385 g          357 t
ORIGIN      
        1 caccattgct atgtctgatg gttatcacac ccgtcacctt tctgctggtg gggccgctat
       61 caacctggat aagcgaactg attgccgccg gttatctctg gctttatcag gcggttcctg
      121 catttgcggg cgcggtaatg ggcggcttct ggcaaatctt cgtcatgttc ggactgcact
      181 ggggcctggt gccgctgtgt atcaataact tcaccgtgct gggctacgac accatgatcc
      241 cgctgttaat gcccgccatt atggcgcagg tcggggcggc gctcggcgtc ttcctctgcg
      301 aacgcgatgc gcagaaaaaa gtggtggcgg gatcagcggc gttgacgggc ctgtttggta
      361 tcaccgaacc agcggtatat ggcgttaatc tgccgcgtaa gtaccccttt gttatcgcct
      421 gtatcagtgg ggctttgggg gccaccatca ttggctacgc gcaaacgaaa gtgtactcct
      481 ttggtttgcc aagtattttc accttcatgc aaaccatccc gtcaacggga attgatttca
      541 ccgtctgggc cagcgttatt ggcggtgtca ttgccatcgg ttgcgcattt gtcggtacgg
      601 tgatgcttca tttcatcacc gctaaacgtc agccagcgca gggtgccccg caagagaaaa
      661 caccagaggt tattacacca cctgagcagg gcggtatctg ttcaccgatg acgggagaga
      721 ttgtgccgct cattcacgtc gctgatacca cgtttgccag tggcctgttg ggtaaaggta
      781 tcgccattct gccctcggtt ggtgaagtgc gttctccggt tgcgggtcga attgcttcgt
      841 tgttcgccac attacacgcc attggcattg agtcagatga tggtgtggag atcctgattc
      901 atgtcggtat cgacaccgta aaactggacg gcaaattctt ttccgctcac gtcaacgtgg
      961 gtgacaaggt caatacaggc gatcggctga tttcttttga tatccctgct attcgcgagg
     1021 ccggatttga tgtgacgacg ccggtattaa tcagtaatag cgatgatttt acagacgtat
     1081 taccccacgg cacggcgcag ataagcgcag gtgaaccgct gttatccatc attcgctaac
     1141 gataaaagga gttaattatg aaagcatttc cagaaacatt tctttggggt ggcgcaacag
     1201 ctgccaatca ggtggaaggt gcctggctgg aagatggcaa agggagcacg acctcagatt
     1261 tacagcctca tggcgttatg ggaaaaatgg aaccgcgcat cctggggaaa gagaatatca
     1321 aagatgtcgc catcgatttt tatcaccgtt acccggaaga tatcgcgtta tttgccgaga
     1381 tggg
//
LOCUS       DAJTXQ010000305         1384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000305 DAJTXQ010000000
VERSION     DAJTXQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.311"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1339)
                     /locus_tag="OO515_004310"
     CDS             complement(<1..1339)
                     /locus_tag="OO515_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6578058.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAJTXQ010000306         1372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000306 DAJTXQ010000000
VERSION     DAJTXQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.312"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..126
                     /locus_tag="OO515_004311"
     CDS             <1..126
                     /locus_tag="OO515_004311"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="catalyzes the hydrolysis of 6-phosphogluconolactone
                     to 6-phosphogluconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6578059.1"
                     /translation="VAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(167..1120)
                     /locus_tag="OO515_004312"
     CDS             complement(167..1120)
                     /locus_tag="OO515_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6578060.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          377 a          314 c          295 g          386 t
ORIGIN      
        1 gtcgccgggc aaaaatctca ccacatctcg gtatacgaaa ttgttggcga gcaggggcta
       61 ctgcatgaaa aaggccgcta tgcggtcggg cagggaccaa tgtgggtggt ggttaacgca
      121 cactaaccgc tgatttaccc ggcgcagtct ctcctgcgcc ggtgtattaa cctatctcct
      181 gtaacgcatg tctctggcgt tcgacgatat tggtccacaa attgtcttta tcgtcagtcc
      241 acaaattaat cagcaaggca aaaaagcgtt ctgctgccgg agaaagcacg gcatctttgc
      301 ggcgaataat ccccaatgtc cgacgtataa cgggttccac cagtggaata ccaataatcg
      361 aagaataggg cgcatggggc attgccagac caggaagcgc cgaaatcccc agtcccgcct
      421 ctaccagtcc taatgacgtc gaaagatgac gcacttcgta aaaccagtcc agcttccagg
      481 gcttgtcggc cagctgttgt tctatcaaca gtcggttgcc gctggaggag cgtacgccaa
      541 tcattttgta acccactaac tcctgccatt ctacaagctg ctttttggcc aatggatggt
      601 cacgccgaca ggccagcacg aacggctcgt tgaccagtgg ggtaaaatca atggatgaat
      661 ttgtgacgtt gttcatgttt atgccaaagt ctgattcgtt acacagcacc gattccatgc
      721 aattattcgt gccttgttcc agaatccgca ctttaatatt gggatacagc tcattaaatt
      781 taccgattgc cagcggtaaa aaataaaata ctgcggtcgg aatacacgct aacgtcacca
      841 taccacgatg ataagcattc atatcacgaa tattaaaaag cgtttcatcg aatttcttta
      901 ttaattcccg cgcctccggg agcaaccttt ttcctgcttt tgttaacgta accttgcgag
      961 ttgtacgctc aaatagttga atatgtaaat cttcctccat cttttttatt cttcgcgtta
     1021 atgcaggttg cgtaatattg agtaatttag cggcattgtt aaacgaactg gactctgcca
     1081 gtatgacaaa tgccttcata cttgataatt catgcttcat tattactccg gaaaatggaa
     1141 gcgacgattt tgggtggctg gccgttaaaa attttaactg catttagcca acttaaatta
     1201 atgaaaaaat gttattaatc gttgagctaa agtcattaga gatgctttgc ccttaatgta
     1261 accatatcgc aataagttat gtttttaaat tgagggcatt attatgaaaa aaataccctg
     1321 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tg
//
LOCUS       DAJTXQ010000307         1368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000307 DAJTXQ010000000
VERSION     DAJTXQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.313"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..292)
                     /gene="tpx"
                     /locus_tag="OO515_004313"
     CDS             complement(<1..292)
                     /gene="tpx"
                     /locus_tag="OO515_004313"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6578061.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGA"
     gene            411..>1368
                     /gene="ycjG"
                     /locus_tag="OO515_004314"
     CDS             411..>1368
                     /gene="ycjG"
                     /locus_tag="OO515_004314"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6578062.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGEL"
BASE COUNT          328 a          317 c          406 g          317 t
ORIGIN      
        1 ctgcgccgca gaaacgagac tgggcgaacg gcagatcggc agagatacac agcacaacgg
       61 tgttgtcgat ctcagttgcc agttggttaa acttgcgtac tgatgcggcg caaacatcgg
      121 tatcaatact cgggaaaatg ttcagcactt tgcgtttacc cgcaaactga ccgagggtga
      181 cgtcagacag atcttttgcc acgagagtaa aagtctgcgc tttgctaccc gcctgcggga
      241 tggaattggc gactgtaacc gggttgccct ggaaatgaac ggtttgtgac atgattatct
      301 ttcctgttta catatagtta acgtcacacc tagtttatgc taactgtcaa taacacagca
      361 aacgctattt gcgcttaatc cgcagaccac cgcgacaaca aggagtaaag atgagaaccg
      421 ttaaggtatt tgaggaagcc tggcccttac ataccccgtt tgtgattgcc cggggaagtc
      481 gcagtgaagc gcgcgtggtg gtggttgaac tggaagaaga gggtattaaa ggcaccggcg
      541 aatgcacgcc gtatccgcgt tatggagaaa gtgatgcctc ggtaatggcg caaattatga
      601 gcgtcgtgtc gcaattagag aaagggctga cacgggagga gttgcaaaaa attctccctg
      661 ccggcgcagc acgtaatgcg ctggattgtg cattgtggga tctggccgcg cgaaaacagc
      721 agcaatcgct ggctgatttg atcggcataa cgcttcccga gacagttatc actgcacaga
      781 cggtagtcat cggtacgcct gatcagatgg ccaatagtgc atcaacactc tggcaggcag
      841 gcgcgaaatt actgaaagtg aagctggata accatcttat tagtgagcgg atggtggcaa
      901 ttcgcacagc tgtgcccgat gcgacgctga tcgttgatgc aaatgaatcc tggcgtgcag
      961 aaggggtggc ggcgcgttgc cagctattgg cggatttagg cgttgcgatg cttgaacaac
     1021 cccttcctgc gcaggatgat gcggcgctgg agaattttat tcatccgttg ccgatttgtg
     1081 ctgatgagag ttgtcataca cgtagcaatc tgaaggcgct gaaagggcgc tatgagatgg
     1141 ttaacattaa gctcgataaa atcggtggtc tgacggaagc gctggcgctg gcgactgaag
     1201 cgcgtgcaca aggtttcagt ctgatgctgg gctgcatgtt gtgtacctct cgtgccatta
     1261 gcgccgcttt accgctggtg ccgcaggtca gtttcgccga tcttgacgga ccgacctggc
     1321 tggcggtaga tgtggaaccg gcgcttcagt tcacgacggg cgaattgc
//
LOCUS       DAJTXQ010000308         1357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000308 DAJTXQ010000000
VERSION     DAJTXQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.314"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..428
                     /locus_tag="OO515_004315"
     CDS             <1..428
                     /locus_tag="OO515_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6578063.1"
                     /translation="FDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            428..718
                     /locus_tag="OO515_004316"
     CDS             428..718
                     /locus_tag="OO515_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6578064.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            715..1269
                     /locus_tag="OO515_004317"
     CDS             715..1269
                     /locus_tag="OO515_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6578065.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          371 a          276 c          387 g          323 t
ORIGIN      
        1 gattcgatta ctggactccg gtcggtgggg cgatcacgcc tcgcgaacga gaactgctgt
       61 ctggtttcgt tgattacctg tgcgaatcag taggtcggga acatacgcca gctctgagcg
      121 aagccgcaga gcaatatctg aatacagttg cgacacgcag aacccgggat acggcattgc
      181 taaagtcgtt tgaggctttt cgcgagtggg taaccattca ggctggattt tacaccgaac
      241 atttttatcc ggacggtagc cgcgggcgtc gggcaaaatc catcgctttt gcgaatatgg
      301 acgaaaccga gtttcagcag gtttataaat ctgttctgaa tgtgctgtgg aactggattc
      361 tgttccgtaa attttcctct ccggaacaag tcgaaaatgt ggccgcgcag ctgctggagt
      421 ttgcgtaatg gtggatttac gtaaagcggc gcgggggcag atgtgcaccg tcagaattcc
      481 tggctactgc aatcacgatc cggaaacgtc tgtgctggcg cattaccgac tggcgggaac
      541 gtgcggaaca gcgataaagc cacacgatat gcaggcagcg attgcctgta gctcgtgcca
      601 cgatttaatc gacgggcggg taaaaaccag cgattacacc aaagaagaat tacgcctgat
      661 gcatgcagaa ggtgtttttc gcacacaaga aatctggaga aaggagggat atttatgatt
      721 tacccaacga atacaggaaa aagcggagaa caccttcgtc tcaccacgct ggaaagtgtc
      781 tggattcagg gaaaactacg tatgtggggg cgctggtcgt atattggtgg cggtaagacg
      841 gggaatatgt ttaacctgat gttgacctct aaaaagctga caaaaacggc aattaacgag
      901 gcgctccgga ggatgaaaaa agcaggtctg aacaagtctg aacttgaggc ttttttgcgg
      961 gatatgatta acggtaagca aaagagctgg ctggcgcatt gtactgatgc agaggcgtta
     1021 tgtattgatc gggtcataag tgaggtgctg gcagagcatc caggattgat tagcgtcctt
     1081 cggcaacggt atgaggggcg ggggatgacc aaacgcaaaa tggctgaact gctgaatgat
     1141 gcacacccga aatggagttt aagaacctgt gaaagacgca ttgagcattg gctaaaggtg
     1201 gcagaattta ttttgtacaa accaatggtt atggcttttg gtatagagaa aaaagttatt
     1261 gctttttgac gtaaaaactg cttcaattct tgtacgcttc gcaaagctgt accgcgaggc
     1321 gaatagcaga catggacatt tgaaagagcc cgcttta
//
LOCUS       DAJTXQ010000309         1333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000309 DAJTXQ010000000
VERSION     DAJTXQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.315"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..186
                     /locus_tag="OO515_004318"
     CDS             <1..186
                     /locus_tag="OO515_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578066.1"
                     /translation="ATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLA
                     QGKRRCQQQFTSQEVQN"
     gene            196..>1333
                     /gene="yicI"
                     /locus_tag="OO515_004319"
     CDS             196..>1333
                     /gene="yicI"
                     /locus_tag="OO515_004319"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6578067.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDK"
BASE COUNT          333 a          316 c          347 g          337 t
ORIGIN      
        1 gccacgatta gcatcattat tgcgctattc acgattgttc cggcgatctg ttatttgctg
       61 agcgcgatta tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg
      121 gaacagctgg ctcagggtaa acgccgttgc cagcaacaat tcacctctca agaagtgcag
      181 aactaaggaa cggcaatgaa aattagcgat ggaaactggt tgattcaacc tggcctcaat
      241 ttgattcacc cgcttcaggt gttcgaggtt gaacagcagg ataatgaaat ggtggtctat
      301 gctgcccccc gtgatgtgcg tgaacgtacc tggcagcttg atacgccttt atttacgctg
      361 cgctttttct ccccacagga aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg
      421 ctgaataacg gtcctcatta tccgctcaat attttgcagg acgtgaaggt cacaatcgaa
      481 aacacagaac gttatgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt
      541 gagttctggt cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa
      601 aataatggct acgtgcagga cacgaataat caacgcaatt atatgtttga gcggcttgat
      661 cttggcgttg gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat
      721 ggccagacgg tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa
      781 aatatcccgt tctacatgac taaccgtggt tatggggtac tggtcaatca tccccagtgt
      841 gtctcttttg aagtgggatc ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa
      901 tatctcgaat actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc
      961 cgctttactg gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact
     1021 tcatttacca ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa
     1081 cgcaatctgc cgctgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg
     1141 tgcgattttg agtgggaccc gctgactttc cctgacccgg aagggatgat ccgccgcctg
     1201 aaagcgaaag ggctgaaaat ctgcgtctgg attaacccct atatcggtca aaaatccccc
     1261 gtctttaaag agttacaaga gaaaggctat ttactcaaac gcccggacgg ttcgctatgg
     1321 cagtgggata aat
//
LOCUS       DAJTXQ010000310         1307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000310 DAJTXQ010000000
VERSION     DAJTXQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.316"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..966
                     /locus_tag="OO515_004320"
                     /pseudo
     CDS             1..966
                     /locus_tag="OO515_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    34..150
                     /locus_tag="OO515_004320"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1008..1208
                     /locus_tag="OO515_004321"
                     /pseudo
     CDS             1008..1208
                     /locus_tag="OO515_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          344 a          313 c          366 g          284 t
ORIGIN      
        1 gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg cggagtttga
       61 ccgcctctgg aaaaaatgat gccactgctg gataagctgc gtgagcagta cggggtcgga
      121 ccgctatgca gcgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga
      181 catcatccgg ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa gaaagagata
      241 cagcgcgtat acgatgaaaa tcacaaggta tacggtgtgc gtaaagtctg gcgtcagttg
      301 ttacgggaag gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatggga
      361 cttgccggtg ttctccgggg taaaaaggtc cgtacgacca tcagccggaa agccgttgcc
      421 gcaggcgacc gcgtaaaccg tcagttcgtg gcagaacgac ctgaccagct gtgggtggct
      481 gattttactt acgtcagcac atggcagggc ttcgtctatg tggcgttcat cattgatgtg
      541 tttgccggat acatcgtggg gtggcgggtc tcatcgtcca tggaaacgac attcgtgctg
      601 gatgcactgg agcaggcgtt atgggcccgt cgaccgtccg gcacggtcca tcacagtgat
      661 aaaggttctc agtatgtatc gctggcctac acacagcggc ttaaggaagc cggattactg
      721 gcatcaacag gaagtacagg cgactcgtat gacaacgcga tggcggagag catcaatggt
      781 ctttacaaag cggaggtaat acaccgtaag agctggaaaa accgtgcaga agtggaactg
      841 gccatactca cgtgggtgga ctggtataac aatcgacgat tgctggaaag gctgggccat
      901 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
      961 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcaac cttcacgata
     1021 aaaaactttt cattaaaggt aaagcggatc aatatccggc acttcattct ctgatatctc
     1081 cgacccgcat taatctgaaa gaaatagaaa ttcactggcg tgaagtgcta cgtctggcga
     1141 cctcaataaa acagggaaca gtgacagcat ccctgatgct gagaaaactt gccagttacc
     1201 ccaaacaaaa gtaaggcagt atgactttcc gtgagacaga tgctggccaa cgtgggatat
     1261 ttaacgcggg ttaacatcgt tgtcagagcc gcatgggtga gctgttg
//
LOCUS       DAJTXQ010000311         1296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000311 DAJTXQ010000000
VERSION     DAJTXQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.317"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..229)
                     /locus_tag="OO515_004322"
     CDS             complement(<1..229)
                     /locus_tag="OO515_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578068.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="OO515_004323"
     CDS             403..1116
                     /locus_tag="OO515_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6578069.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..>1296
                     /locus_tag="OO515_004324"
     CDS             1148..>1296
                     /locus_tag="OO515_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578070.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHW"
BASE COUNT          347 a          306 c          288 g          355 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggca
//
LOCUS       DAJTXQ010000312         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000312 DAJTXQ010000000
VERSION     DAJTXQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.318"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1112
                     /locus_tag="OO515_004325"
                     /pseudo
     CDS             1..1112
                     /locus_tag="OO515_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAJTXQ010000313         1250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000313 DAJTXQ010000000
VERSION     DAJTXQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.319"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..154)
                     /locus_tag="OO515_004326"
     CDS             complement(<1..154)
                     /locus_tag="OO515_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCR6578071.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWM"
     gene            complement(477..1250)
                     /locus_tag="OO515_004327"
                     /pseudo
     CDS             complement(477..1250)
                     /locus_tag="OO515_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          404 a          187 c          248 g          411 t
ORIGIN      
        1 gcatccagac acagaaggca agaagtagac aactgactga tatccagagg tttcttcgag
       61 caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag tcgcgtaaaa
      121 gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc acacattggt
      181 aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa ttaaggatga
      241 attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg ggcagaatgg
      301 ggattaagta gccagatatg tgttactaaa tgtaactaac aagctaacct tcaaacgggg
      361 ttaatctttg aacgtatgtc atgtttcaac ccttcagatc gtgaatctaa agggttacat
      421 attaactata ttatcaacaa gaccagacac ttatagaatt agtgatgatt tattcactaa
      481 aaaggccatg aactggagga aaacgatctg tccagaaggt ctatcggttc ctctcttatt
      541 gcctgattgt aataatcgtt attacagtaa gtattgttta tttcatctaa ggtcatgttt
      601 ttaactttta tattcctctg gtttacaact tcctctgtct tgtaagtgtg tatattatat
      661 ggggtatatg ctatttttaa taggatagtg aaacaattcg ttatccacat ctattccgtc
      721 agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa gcttaagtgt
      781 tggggaaaac aaattaaata acatgagctg catagtaggg caattgttct tcccagaaac
      841 atttaaaaaa tttaagtttt tgctctgttt tattatggga aataaagttc caatatcgga
      901 tgtattatta gtatcaatgt gggttatttt attgttgtcc agtcttaaac ttttaataga
      961 tggaagacaa gtcaggaaat caagatttat atttttcaac tggttgttag cagcgtttaa
     1021 tgtctgaaca ttgcagaagt tgctatagaa tattttaatt tcagaaagca ggtttttgtt
     1081 aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta gtttattatg
     1141 ggatagatcc agcagtaaga gttaattttt attacctgca acaatatcag ataattgatt
     1201 atggctgaga ttcaggtatt gcagacattc gcaagattct aattgcacaa
//
LOCUS       DAJTXQ010000314         1207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000314 DAJTXQ010000000
VERSION     DAJTXQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.320"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..>1207
                     /locus_tag="OO515_004328"
     CDS             join(52..301,301..>1207)
                     /locus_tag="OO515_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6578072.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          343 a          294 c          332 g          238 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gtgccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg
     1141 aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact
     1201 cggtggc
//
LOCUS       DAJTXQ010000315         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000315 DAJTXQ010000000
VERSION     DAJTXQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.321"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..295)
                     /locus_tag="OO515_004329"
     CDS             complement(<1..295)
                     /locus_tag="OO515_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6578073.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(343..915)
                     /locus_tag="OO515_004330"
     CDS             complement(343..915)
                     /locus_tag="OO515_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6578074.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          380 a          246 c          220 g          344 t
ORIGIN      
        1 aaggttctaa tcgtgatact ggcggaataa cggtaaacgg cgctttgctt ttatcctctt
       61 tattaaatgg atctacccaa gcctggatga gataaggtat tttttcttct ttgctaaata
      121 cagaaataga tttatcagca ttattactta agtaaaccac ccgcgtacca ccaatgacaa
      181 cgcctgcata agttaatgct ggttggccta aaaaaacgct gagtataatc gcggtaaata
      241 acttacatga aactgttgtt ttaaagagcc tgattaaaca actttttctg gacataacga
      301 ctccctggcc aaagccaact gaataaaaag gcccttgata aattatttct tatattcgac
      361 agtaaattgt gaatcagcgt tcgccgtacc agccgttacg gtcgcattgg ttgccacata
      421 gcgtgcaata aacttcattt cagccttgcc ttcagcatcg atagcggtat aagcagagtc
      481 gttataaagt ttaacctgcg aattatcgga ctggttaaaa atacgaatac caacgccggt
      541 tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg ttgcctgctg taagcggcgt
      601 acccactttc aggtcgccgt cgccgccagg cgcttctgta ccatcaaaac gaacggctgc
      661 ttgagtgtac gttgccgggc aattttcaag tttaatactg aacttctgtg gcgacgccag
      721 actacctaca cctgaaaatg catttttact gactttaccc agtgggacaa tttgatcggc
      781 tgtatcggag gtaacttcac aagtagtgtc gacaatttca ccgacgaagt gaacaacacc
      841 atcttctgcc agtgcatgcc cggccagcat tagagatgcc agagtgaatg caccgcctat
      901 aatattacgc ttcataaaaa ctccattgat ataatatatc ctttaccatg aatcatcact
      961 tatattaata agtgtatttt tgattcaaag gaatagtaca ccaaagagac ggctttatct
     1021 aaaaataata ttaattacgc aaaaataaaa tataaattca ttgtttaaat attatttaaa
     1081 cagtaaaaaa tcaagtttgt tatcagacat taatgataat tcagaatata tttatataga
     1141 aagatagata aatttattaa tgtcagttat aaaaaagggc caccggtcgg
//
LOCUS       DAJTXQ010000316         1175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000316 DAJTXQ010000000
VERSION     DAJTXQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.322"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1175
                     /locus_tag="OO515_004331"
     rRNA            <1..>1175
                     /locus_tag="OO515_004331"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          297 a          262 c          364 g          252 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      721 gcatggagcc ggccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg
      781 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
      841 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      901 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      961 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
     1021 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
     1081 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
     1141 acgcgagctg ggtttagaac gtcgtgagac agttc
//
LOCUS       DAJTXQ010000317         1154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000317 DAJTXQ010000000
VERSION     DAJTXQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.323"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            278..>1154
                     /gene="nadA"
                     /locus_tag="OO515_004332"
     CDS             278..>1154
                     /gene="nadA"
                     /locus_tag="OO515_004332"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6578075.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          313 a          277 c          278 g          286 t
ORIGIN      
        1 cgaagcgagt catcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt
       61 gctattcagg catcctcaat ttcactttgt aaacctgatg acatcgtcag agcttactgt
      121 gcaagcaact ctacgtcggt ggaattaggc gtaattcgag tgacttttcc ccaccattcg
      181 actatcttgt ttagcatata aaacaaatta cgccgatagt agcgaatatt acgctaatgt
      241 cggttttaac gttaagcctg taaaacgaga tggtaagatg agcgtgatgt ttgatccaga
      301 cacggcgatt tatcctttcc ccccgaagcc gacgccgtta agcattgatg aaaaagcgta
      361 ttaccgtgag aagataaaac gtctgctaaa agaacgtaat gcggtgatgg ttgcccacta
      421 ctataccgac cccgaaattc aacaactggc agaagaaacc ggtggctgta tttctgattc
      481 tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct acgttgttag tcgctggggt
      541 gagatttatg ggagaaaccg ccaaaattct cagtccggaa aaaacaattc tgatgctgac
      601 acttcaggct gaatgttcac tggatctcgg ctgccctgtt gaagaattta acgcattttg
      661 cgatgcccat cccgatcata ctgtcgtcgt ctacgccaac acttctgctg cggtaaaagc
      721 gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa cttattgatc atctggatag
      781 ttttggtgaa aaaatcatct gggcacccga caaacatctg gggcgttacg tgcaaaaaca
      841 gacgggcgca gacattttat gctggcaggg tgcctgtatt gtgcatgatg aatttaagac
      901 tcaggcgtta acccgcttgc aagaagaata cccggatgct gccatactgg tgcatccaga
      961 atcaccacaa gctattgtcg atatggcgga tgcggtcggt tccaccagtc aactgatcgc
     1021 tgctgcgaaa acattgccac atcagcggct tatcgtggca accgatcggg gcattttcta
     1081 caaaatgcag caggcggtgc cagataaaga gttactggaa gcaccaaccg caggtgaggg
     1141 tgcaacctgc cgca
//
LOCUS       DAJTXQ010000318         1117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000318 DAJTXQ010000000
VERSION     DAJTXQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.324"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..1115
                     /locus_tag="OO515_004333"
     CDS             84..1115
                     /locus_tag="OO515_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6578076.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAJTXQ010000319         1111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000319 DAJTXQ010000000
VERSION     DAJTXQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.325"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..438
                     /locus_tag="OO515_004334"
     CDS             13..438
                     /locus_tag="OO515_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6578077.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1111
                     /locus_tag="OO515_004335"
     CDS             463..>1111
                     /locus_tag="OO515_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6578078.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSD"
BASE COUNT          310 a          252 c          257 g          292 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat g
//
LOCUS       DAJTXQ010000320         1110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000320 DAJTXQ010000000
VERSION     DAJTXQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.326"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            131..982
                     /gene="hyi"
                     /locus_tag="OO515_004336"
     CDS             131..982
                     /gene="hyi"
                     /locus_tag="OO515_004336"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6578079.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            901..1110
                     /locus_tag="OO515_004337"
                     /pseudo
     CDS             901..1110
                     /locus_tag="OO515_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Plays a crucial role on both purine and pyrimidine
                     metabolism; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
BASE COUNT          300 a          234 c          288 g          288 t
ORIGIN      
        1 tgagcgtgtg accaatattt cgatgggcag cgaactggat aacgtcatgg aatttgaaga
       61 tatcgccgat aacgcagtgg acgcaccgac tgaaacctgc ttcatgcact atgaataagg
      121 gagataaata atgttacgtt tctctgctaa tttatcgatg ttatttggtg aatatgattt
      181 tctcgcccgt tttgagaaag ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc
      241 ttatgactac gacattgaag aattaaaaca ggtgctggcg agtaataaac tcgaacatac
      301 gctgcacaat ctaccggcgg gtgactgggc ggcgggggag cgcggtattg cctgtattcc
      361 tggccgtgaa gaagagtttc gggatggcgt agcagcagcg attcgttatg cccgtgcgct
      421 ggggaataaa aaaattaact gtctggtcgg taaaacgccg gctggtttca gcagtgaaca
      481 gattcacgca acgcttgtag aaaacctgcg ttatgccgcg aatatgctga tgaaagaaga
      541 tattttatta ctgattgaac ctattaacca ttttgatatt cctggtttcc atctcaccgg
      601 aactcggcag gcgctgaaat tgattgatga tgttggttgc tgcaatttga aaattcagta
      661 tgacatttat cacatgcagc ggatggaagg tgaattaacc aacaccatga ctcagtgggc
      721 cgataaaatt ggtcacctgc aaattgctga taatccgcat cgcggcgaac cggggaccgg
      781 agaaattaat tatgattatc tctttaaggt aatcgaaaat tctgactaca acggttgggt
      841 tgggtgtgaa tataaacccc aaaccaccac ggaagccggt ttacgctgga tggatccgta
      901 ctttctgaac cgggtcgtag ccactgggaa ggttatgaaa ccggtactcg cgcagcggca
      961 aaaggtggta tcaccaccat gatcgaaatg ccgctcaacc agctgcctgc aacggttgac
     1021 cgcgcttcaa ttgaactgaa gttcgatgcc gctaaaggca agctgactat cgatgcggca
     1081 caactcggtg gcctggtgtc ttacaacatc
//
LOCUS       DAJTXQ010000321         1106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000321 DAJTXQ010000000
VERSION     DAJTXQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.327"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..669)
                     /gene="cadA"
                     /locus_tag="OO515_004338"
     CDS             complement(<1..669)
                     /gene="cadA"
                     /locus_tag="OO515_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6578080.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTA"
     gene            complement(749..1096)
                     /locus_tag="OO515_004339"
     CDS             complement(749..1096)
                     /locus_tag="OO515_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCR6578081.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          322 a          246 c          263 g          275 t
ORIGIN      
        1 cgcagtggaa gtaccgttgg tcaccatgta gctgcggtct gcgttaaaga cgcgagcgat
       61 atactgttct gcttctttgt gtggaccact atgatccagc agagaaccca gttcagatac
      121 tgaaatggaa atatcagatt tcatggtatt cggaccaaag aaatcataga acaggctacc
      181 taccgggctt ttctggaatg cagtaccgcc catgtgacca ggagtacaga aagtatattt
      241 accttcacga acatatttaa acagtgcttt agtcagcgga ggcagaatag tgttgatata
      301 ttcgtcagtg gtctgcttga tcttattagc aatatcttca gcagcaccca gcgcatattc
      361 aaagaagcta atctgtaaac gcaggtcatt caggcttaca tcgagagtgg aatacgtatt
      421 agcgaacgcg tacaacggca ggttctcgtt cattttgcta atttcttcgc acagctcgag
      481 attatattta tcccagtcaa aaataacgcc gcacagacgc gcattgtttt cgatcagttt
      541 taataagtcg tcacggtcgt tcgggtaaac aatctggaag ttcagacgtt caagcgcgcg
      601 atgaagttca cggatgggtt cttctttaaa ataaaccccc atgtgattca atattgcaat
      661 aacgttcata gtcatatctc caggtaaaaa aggcccctcc caacacatgg gacaaaatga
      721 aaggaggagc ctcggaaaat acttttaatt aatgtgcgtt agacgcggtg tggttatcca
      781 ttgagtggct ctggcgctcg tgcattttgc gagcgtagaa catcaggata atcaggctga
      841 cgatgaaggt acctgccagc tcgaaggagc ttgcgcccat cagcgcgatg aagcagaaca
      901 cacaacccag tacagagcag atcaggctga caaagttgcg gatgttaacg ccttcaaaac
      961 gaatcaggtc aacgcaagag tagaagtacg gcagcatagt cagcagtact gcgataccgg
     1021 tcagttcacc gaacaggtca gatgctttac caccggcaga gttcatcaga gtgatgagga
     1081 tcatcagggc agtcattttc actgca
//
LOCUS       DAJTXQ010000322         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000322 DAJTXQ010000000
VERSION     DAJTXQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.328"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1074
                     /locus_tag="OO515_004340"
     CDS             join(<1..167,167..1074)
                     /locus_tag="OO515_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCR6578082.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAJTXQ010000323         1080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000323 DAJTXQ010000000
VERSION     DAJTXQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.329"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..371
                     /locus_tag="OO515_004341"
     CDS             6..371
                     /locus_tag="OO515_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6578083.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            368..1033
                     /locus_tag="OO515_004342"
     CDS             368..1033
                     /locus_tag="OO515_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578084.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
BASE COUNT          289 a          230 c          296 g          265 t
ORIGIN      
        1 ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc ggtggtcgct
       61 gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta aaaccggtca
      121 ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac atggtaccag
      181 catgccaccc atgcaatctg cacaagcatt gcagtagcct ggaagattat cgacgaatta
      241 tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg
      301 gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat caagaagggg
      361 ctacggcatg accactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg
      421 cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta tcgctctggc
      481 atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca tgtgtattga
      541 cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc tttatgctgc
      601 cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga aggtgacgtt
      661 tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca aagcggaagt
      721 tgagtgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa caccgcttta
      781 cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc
      841 actgtcagat tcatcgcttg gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc
      901 tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa
      961 cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa
     1021 gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag
//
LOCUS       DAJTXQ010000324         1058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000324 DAJTXQ010000000
VERSION     DAJTXQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.330"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..581
                     /locus_tag="OO515_004343"
     CDS             162..581
                     /locus_tag="OO515_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6578085.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            581..>1058
                     /gene="umuC"
                     /locus_tag="OO515_004344"
     CDS             581..>1058
                     /gene="umuC"
                     /locus_tag="OO515_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6578086.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          266 a          226 c          281 g          285 t
ORIGIN      
        1 tggatgagcg tcgtcgccag aaggccacgt gagcacagga taagagaacg aaaaatcagc
       61 agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg gcaagaacag
      121 attactgtat ataaaaacag tataacttca ggcagattat tatgttgttt atcaagcctg
      181 cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc
      241 cttcaccggc agcagattac gtcgaacagc gcatcgatct gaatcaactg ttgatccagc
      301 atcccagcgc gacttacttc gtcaaagcaa gtggtgattc tatgattgat ggtggaatta
      361 gtgacggtga tttactgatt gtcgatagcg ctattaccgc cagccatggt gatattgtca
      421 tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg acggtacagc
      481 ttattcccat gaacagtgcg tactcgccca ttaccatcag tagcgaagat acgctggatg
      541 tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgctc tctgtgatgt
      601 aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg gtaaaccggt
      661 ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg caaaggcgct
      721 gggtgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc gctgtggtgt
      781 ggtttgcttt agcagcaatt atgagcttta cgcagacatg agcaatcggg tgatgtcgac
      841 gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat tctgcgatct
      901 gacaggtgtg cgtaattgtc gcgatctgac tgattttggc agagaaattc gcgcaacggt
      961 gctacaacgt acccatctta ctgttggtat ggggatcgcc cagaccaaaa cgctggctaa
     1021 gcttgccaat catgcggcaa aaaaatggca gcggcaga
//
LOCUS       DAJTXQ010000325         1049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000325 DAJTXQ010000000
VERSION     DAJTXQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.331"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..193)
                     /locus_tag="OO515_004345"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OO515_004345"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..916)
                     /locus_tag="OO515_004346"
     CDS             complement(197..916)
                     /locus_tag="OO515_004346"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6578087.1"
                     /translation="MTIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
BASE COUNT          283 a          222 c          196 g          348 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggc
//
LOCUS       DAJTXQ010000326         1040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000326 DAJTXQ010000000
VERSION     DAJTXQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.332"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..689)
                     /locus_tag="OO515_004347"
     CDS             complement(<1..689)
                     /locus_tag="OO515_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6578088.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQT"
BASE COUNT          311 a          174 c          219 g          336 t
ORIGIN      
        1 gtttgttgta acaaagtttg cattattctg ggagatagtt gattttctct aagattgaca
       61 acgctatcag aactgaggcc aagtatgctt tccggaattg ccgtgatccg gttctgatta
      121 agccagaata ttcttataat atgatcatct ctttctggaa aatctggtat tacttccaaa
      181 gcatttctgg ctgcatcaag taattccaat gacattggta acctcggaag tacagacagg
      241 tgattgtcac ttacattaat atattctagt gacgcaggta attcaggaag tgcgaacaaa
      301 tggttatcac tcacatttat atattccagc gacactggta attcaggaag tgcagataat
      361 tgattactgc ttgcattcag ctctttcaat gcccttggta gctcggggag cattgatagt
      421 tggttattgc ttacattaat ttcatcaaga ttgtcaggca atcgtggtag agatctgaga
      481 cctaaacaag ataagtccaa cgatgtttca ctgttctcca gacataattg gagccgggta
      541 aaagcagttt ccctgttttc tccggaaatg ctgtttttag tccattcaac ccattcggag
      601 agataattat tgtgaacatt gtcgattgat gtagttctgt aaaaagagac gtttccagtg
      661 gatagggggg gattatttac aggaagcata ataacctcgc agaaggatat tctgataaaa
      721 tgtgatactg ggaaataata agctgaaata aaatgtttca ggattgaaat ttattgtttt
      781 cattttaagg aagagatgtt gatttaactg taaaaaaacg atactatttt cttagatggt
      841 aaatgtctcg cctgtgctat attttgtttt tcggttagtt aaatatcgta cgttcatatt
      901 tgaacgttct gccggaatgc attatcaata gaggtaaagt cgcaacccca aatcgtaaag
      961 gaaaccgtag cacgtcttaa gcaagaacgt gctgcggttg gatgctattt tttccctgaa
     1021 gcggaaaaca ttactacagt
//
LOCUS       DAJTXQ010000327         1038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000327 DAJTXQ010000000
VERSION     DAJTXQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.333"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..562)
                     /locus_tag="OO515_004348"
     CDS             complement(<1..562)
                     /locus_tag="OO515_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6578089.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIID"
     gene            complement(580..>1038)
                     /locus_tag="OO515_004349"
     CDS             complement(580..>1038)
                     /locus_tag="OO515_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6578090.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
BASE COUNT          267 a          249 c          219 g          303 t
ORIGIN      
        1 tatcgatgat atctgtgatt aatgctgcga cactcttacg tgatacgatc gtacctttga
       61 atggttcatt gcgtgaggta agctcataat caataatatc ttcatcagtc agccaggcag
      121 ggcgaagaat tgtatattcc agtccagaag cctcaattgc atccgcagcc ctgcggaatg
      181 gttttagggg ctcaccaata actgcattat tccattcgcc aaattttcct ggaacttcat
      241 cataaatacc gagggaaaga acaaagatca gacgtttaac atcacaggct ttcatcgcag
      301 cgatcactga atttgcctga atatctaaat cttcccccgt aaggttggca tacacaacgt
      361 cctgtccctg catggcctgc ttcagtgctg catgattcag tacatctccc ataataatct
      421 tgctattagt tggatagggt ttgtggattt ttgctggctg acgggcaaat aaagtttgtt
      481 taatggtttg tttatctgcc agttgattaa tcacatggcg ggctatctga ccgccagcac
      541 caagaatcag tacatttttc ataatatttc tcatgaaact tatcccagat gggtgttgaa
      601 aaaaggaata actttttcca gcgcctcctg aactgcttcc ggtttgtcat acagttcata
      661 atgtgagaaa tcgggtaatg aaactaactg acgatcctgc gaaaccatcg cccggccata
      721 gacttccagt ccatcacgat aagcaccgaa ggcaccgact ttttcaccaa ctactaccat
      781 caccggctga gtcagtaaca cttcagtaaa tgcgaaggca tcccaggcca gcgttttctg
      841 tgcatgtgag aacaacattt ttgtcgcacc tcccggttgt tgcccgcgtg gtgttttata
      901 gtagtcagtt gcttcataaa catcgcgttc ggtcagtcca tgggcttttg ctgcatccag
      961 gctggcggga agtaattcgt tgatctgtaa ttctccacca cgagcctctt tggttcgttg
     1021 cgacgccatt gcattcaa
//
LOCUS       DAJTXQ010000328         1018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000328 DAJTXQ010000000
VERSION     DAJTXQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.334"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..112
                     /locus_tag="OO515_004350"
     CDS             <1..112
                     /locus_tag="OO515_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6578091.1"
                     /translation="AIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
     gene            152..940
                     /locus_tag="OO515_004351"
     CDS             152..940
                     /locus_tag="OO515_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6578092.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          261 a          238 c          264 g          255 t
ORIGIN      
        1 ggctattcgt tttgccagtg cggttgccgc gatgaaatgt acccagcctg gcggtcgggc
       61 cggcattcct aatcgtgagc aaaccgaatc atttttgtca ctttatgcgt aaaatggtaa
      121 acatctaccg aggaggggca agggagtatt catgagtatc atcgaagtga caggtaatcc
      181 acgccatgac caactcgttc atctaatcgc agaacgcggt tatatgaata ttgaagaact
      241 ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg gatatccgaa aactcagtga
      301 gcagggcttg atcacccgtc atcatggagg ggccgggcgc gtctccagcg tcatgaatac
      361 cgcttttgag caacgggaac tttcgctcac cgccgagaaa cgggcgatcg ctgaggcagt
      421 cgccgattac cttcccgaac gctgtaccgt ctttatcacc atcggtacaa ccgtagaagc
      481 cgttgccagg gcattactca accggcgtga tttacgcatt atcactaaca gcctacgtgt
      541 ggcacagatt ctttataaga atcaggatat tgaagtgatg gtgccgggag gcactttacg
      601 cgctcataac ggcgggatta tcggcccagg agccgtggat tttattgaag gcttccgcgc
      661 agattattta atcaccagta taggtgccat tgaacatgac ggcaccctac tggaatttga
      721 tgtaaatgaa gcgttagtcg cgagaacgat gattaaacat gcgcggaata cattgttagt
      781 ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca attggcaatg cacggaatgt
      841 cagggctttt tttactgatg ccccgcctcc caattctttc tgccagttgt taagtgaaga
      901 gaatgttgaa ctggtggttg ccgagcaaga agtatcctga aattgaaatt tcggaaaatc
      961 cgctgggaat ttcagcggat tttgttttcc ataagcaaaa aaaacctgcc agcgatgg
//
LOCUS       DAJTXQ010000329          999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000329 DAJTXQ010000000
VERSION     DAJTXQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.335"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..393)
                     /locus_tag="OO515_004352"
     CDS             complement(<1..393)
                     /locus_tag="OO515_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HCR6578093.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFT"
     gene            complement(450..>999)
                     /locus_tag="OO515_004353"
     CDS             complement(450..>999)
                     /locus_tag="OO515_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6578094.1"
                     /translation="VLDTDQFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSD
                     HSPCPPEMKAGNIMEAWGGIAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFG
                     LQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDV
                     IYDIEQGFPVAPKGQFILKHQQ"
BASE COUNT          264 a          260 c          252 g          223 t
ORIGIN      
        1 cgtaaataac cgtgccaggc gatggcctaa tccgctaaaa ccaatcaaca tcgtcagcac
       61 gccggagagt gcaatcccca ccgccagact ggtagcgata tcgttgatcg gtgtcccgcg
      121 cgatgcttcg ccaagggtga tagtaaggat ggttccccac cacaggccac ccggcccttc
      181 cataatagcg cgacgatgac cgcagaacgc ctgagcgaag caggccagtg cggtagcaag
      241 aaaagcatat tgcgtgagcg taagcaggct actttgcggc aactgaaaag cagaaagtag
      301 cgtaggagga accacaaccg tgttgcaaaa aataaagaaa aaccactgaa atcctgataa
      361 caggctttcg cggctgactg caaaattgaa cataaacaca ccttaaccgg agaatgcccg
      421 ccgcaaggac gggcattgca ggggccagat tactgctgat gtttaaggat aaattgacct
      481 ttcggcgcaa cagggaagcc ctgttcaatg tcgtaaatca catcaccacg taagatggtt
      541 ttcgtgatac gcgcgccaat ggtacggcca acatacgggc tgactttgtg gcgatattcc
      601 agatcgtcat tggtaagaac atagctgcta ttcggctgaa tgaagacgaa gtcggcatct
      661 tttcctgggg cgatacggcc tttttgctgc agaccgaaaa tatctgctgc gttagtcgcc
      721 attaatttgc cgaacattgg cagagacatt ccgcgtttct gtaccgcttc atcgaacatc
      781 acgtccatac agttttgcag accggcaata ccgccccatg cttccatgat gttgccggct
      841 ttcatttccg gagggcatgg tgagtggtcg gaaaccaggc agtcgatttc accgttaaac
      901 agtttttccc acatgccttt ctggttttcc agatcgcgga tcggcggtga acacttcgcc
      961 agagtaccga tttcttcgaa ctgatcggta tccagtaca
//
LOCUS       DAJTXQ010000330          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000330 DAJTXQ010000000
VERSION     DAJTXQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.336"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..548)
                     /locus_tag="OO515_004354"
     CDS             complement(<1..548)
                     /locus_tag="OO515_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6578095.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIA"
     gene            complement(671..>990)
                     /gene="tabA"
                     /locus_tag="OO515_004355"
     CDS             complement(671..>990)
                     /gene="tabA"
                     /locus_tag="OO515_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6578096.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          281 a          227 c          176 g          306 t
ORIGIN      
        1 gctatttcct tgttagcttc gcgttggtaa catgccagaa tcttgttagt aatcaggtgt
       61 aatgaactct catcttttct ttcaaaggac tttccttcat aacgattagt cgaaccatca
      121 ggttgttgaa atataatagc cgtatgtcct tgacgagtcc ccatagtagt gaaagcggaa
      181 agtgtgattt ctatttccac cttagataaa tccgctcgtt ccccttcagg attattcata
      241 acaaacttca ttatctcact gccatcatta tttttgatat aagatgagga ctcaatatga
      301 gccatcatat ttcttcccgc ttcgttggag cgtttgctaa aatccacata acctagagta
      361 aatattttta ccaaaaattc caaaaatgtc ttaggatcaa ggatatttct tgtgttatcc
      421 tgcccctcgg cattacgggc aaaattattc attccaacac gccaggtatt taaaacttca
      481 cttttttgtt taaaattgtc ggctaatgta atgtgttgag aataattcaa atctgatatt
      541 ttgctcatga cgaactcctt gagttaataa gaacgtctaa gcattacaaa ttttactcac
      601 aaaaaaatat caaaaaacgc tcttttcaca aactcgccgt aaacctggag ttatttacgg
      661 cgaaaagtca tcacgccatc agcatcttca ctactgcttt gcgaacctgg gctggtgcac
      721 ccactgcgca cagcggttta tgcacttccc ccggataaaa cacaacaaaa tcaccttcat
      781 taaggataac tgttttctca tcaacgcctt ccggcaaaaa tgcgatgtct ttatcagcta
      841 accagtcggt atccggcgtg cctgcaggtt gcgtgctgaa ggtcatgcct tcctgacctt
      901 ttaacacaat ctgaatgtcg agatagcggg catggtactc cgtacggcga gcttcgtgcg
      961 gctcggtcat atcttccgag ataagataaa
//
LOCUS       DAJTXQ010000331          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000331 DAJTXQ010000000
VERSION     DAJTXQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.337"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..613
                     /locus_tag="OO515_004356"
                     /pseudo
     CDS             1..613
                     /locus_tag="OO515_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          361 a          175 c          160 g          271 t
ORIGIN      
        1 atgttccagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
       61 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
      121 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
      181 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
      241 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
      301 ttaccagaga tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat
      361 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac
      421 tatagattta aacatagaag actctagctc agaggaattc ctttttgata atagcctcga
      481 gaaagacgaa aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc
      541 atataactca gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt
      601 aaatgcaata tagacataat aacttcttac aaaatctata attaataccg tagatattca
      661 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      721 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt
      781 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag
      841 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      901 cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc
      961 caaaagc
//
LOCUS       DAJTXQ010000332          966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000332 DAJTXQ010000000
VERSION     DAJTXQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.338"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /locus_tag="OO515_004357"
     CDS             <1..781
                     /locus_tag="OO515_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578097.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
BASE COUNT          287 a          226 c          221 g          232 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcac
//
LOCUS       DAJTXQ010000333          955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000333 DAJTXQ010000000
VERSION     DAJTXQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.339"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>955
                     /locus_tag="OO515_004358"
     rRNA            <1..>955
                     /locus_tag="OO515_004358"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          262 a          206 c          307 g          180 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacag
//
LOCUS       DAJTXQ010000334          918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000334 DAJTXQ010000000
VERSION     DAJTXQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.340"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..221)
                     /locus_tag="OO515_004359"
                     /pseudo
     CDS             complement(1..221)
                     /locus_tag="OO515_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(361..>918)
                     /locus_tag="OO515_004360"
     CDS             complement(361..>918)
                     /locus_tag="OO515_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6578098.1"
                     /translation="AVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRL
                     HIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKF
                     ATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVFKAG
                     LRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          260 a          206 c          151 g          301 t
ORIGIN      
        1 atggtatcgt gaacaggaat accattttca aaatcaccat attgttttaa aaaatcgaga
       61 tgtgtttccc caaaatcctc tatatcttcc cagccttctg caccagaaat aacggcacaa
      121 atagtcaaca gtagaatatc cgataattta tgttccactt tccaggcttg tttttaatcg
      181 gggataatag aaatatgttc catcaatttt ttaagttcca ttttgttctc cttaattatg
      241 taaggagtat ttgatcatgt ataagcaata aaaaacagct tcaggtaata aggaatatct
      301 caatttttaa acataaaatg cgaattattt agtacaaaaa gtgaactcct cagggcaaga
      361 ttacgaaagt ccgctccccg caaggactga cgcgagatag tttctgtcca tggctgcttt
      421 tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa cattaatagc
      481 aatgtgccgt atccctgaaa ataattctgc tgcatttcct cttcttattt tgcagtcgtc
      541 ttcattcatt accacgtcca gacgccagtg cagattattc tccacgtgcc agtgatttcg
      601 aatcgctgtc gcaaacttct cagcggttaa atcagcagaa ctgatataat atctgaccat
      661 catttcgggc tctttctttt gctctgctat tattgagcga aaggagactg ccacgcataa
      721 ttttttcagc cctttccatt caaacgtgaa atcaataagt tcatcaggga catcgcaaac
      781 aatatgaaga cggatttctt ctctgccgtg actcttttca ctcattgcgg aactgtcatg
      841 cgctggatta tttaattctg tcagcggaaa ttttttctca aaggctttat ttagccgccc
      901 ctggtttcct tttaccgc
//
LOCUS       DAJTXQ010000335          916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000335 DAJTXQ010000000
VERSION     DAJTXQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.341"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..590)
                     /gene="ydcO"
                     /locus_tag="OO515_004361"
     CDS             complement(<1..590)
                     /gene="ydcO"
                     /locus_tag="OO515_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6578099.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTMTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIA"
     gene            643..>916
                     /gene="sutR"
                     /locus_tag="OO515_004362"
     CDS             643..>916
                     /gene="sutR"
                     /locus_tag="OO515_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6578100.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLF"
BASE COUNT          241 a          251 c          228 g          196 t
ORIGIN      
        1 tgcgcgataa cgatcacgat cccaataatc atcgcggcaa ttaccgcata gcgcggcgca
       61 acggccttgg ttgccagcca taccagcaac atacttccac acaacgtaaa ttgaccgtcc
      121 agactggcaa acgcctgtaa accaaagcgt aataaaatcc cggcaagcat tgccgccgca
      181 agcgagtgcg gaataatgcg catcagacga gcaaagagtc ccgttatgcc gcagaggact
      241 attaacgcgt tggtgacaat aaaaacgccg atggcttcgt taagtgttag tccctgcaat
      301 ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg tgagaacagg tacgcgatac
      361 cataatgtca gagtcatcgt actgacgccc attgccagcc ccagcgccgt catccagcca
      421 gagatttgtg cagtggtggc tccggcgaca atcgctgctt gccagattat tgccgctgaa
      481 ctggcgtagc caattaatac cgccagaaac cccgccagta gcgtgggtgg aggaatagaa
      541 aacagacgca tagtcacctc gtgcgttata gcgtacaggc accgtagcat ttgtccgtta
      601 taacgcacaa gtgataaact tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc
      661 tttttatcca ctacacttaa acaactacgc cagcagcgcg gctggagtct ttcgcgactg
      721 gcagaagcga cgggcgtttc taaagcaatg ttagggcaga ttgagcgtaa tgagtccagt
      781 ccgacggtcg cgacattatg gaaaattgcc accgggttga acgtaccatt ttcaacattt
      841 atttctccgc cgcagtccgc tatgccttca gtttatgatc cacaacagca ggcgatggtg
      901 atcacttcgc tgtttc
//
LOCUS       DAJTXQ010000336          915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000336 DAJTXQ010000000
VERSION     DAJTXQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.342"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>915
                     /locus_tag="OO515_004363"
     CDS             <1..>915
                     /locus_tag="OO515_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6578101.1"
                     /translation="DEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVR
                     EKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVE
                     LSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWA
                     YVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRIT
                     EAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTK
                     PLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          194 a          255 c          299 g          167 t
ORIGIN      
        1 tgacgaaaag cgactgttgc ctgcggcgcc gtgctgcccg aactgcggcg gttcactgag
       61 ctatctgggc gaggataccg ccgaacagct ggagttgatg cgtagcgcct tccgggttat
      121 ccggacggta cgggaaaaac atgcctgtac tcagtgcgat gccatcgtgc aggcacctgc
      181 accttcgcgg cccatcgagc ggggtatcgc cggaccgggg ctgctggccc gcgtgctgac
      241 ctcgaagtat gcagagcaca ccccgctgta tcgccagtca gaaatatacg gccggcaagg
      301 tgtggagctg agccgttcac tgctgtcggg ctgggtggat gcatgctgcc ggctgctgtc
      361 tccgatggaa gaggcgcttc atggctatgt gctgactgac ggtaagctcc atgttgatga
      421 cacgcctgtc ccggtgctgt tgccaggcaa taagaaaacg aagaccgggc ggttgtgggc
      481 gtatgttcgt gacgaccgta acgccgggtc aacgctggcg ccggcggtgt ggttcgctta
      541 cagcccggac agaaaaggca tccatccgca gacccatctt gcggggttca gtggtgtact
      601 gcaggcggat gcatacgccg ggttcaacga gctgtaccgg gatggccgga taacggaagc
      661 cgcctgttgg gctcacgccc gccgtaaaat ccacgatgtg cacgttcgca ccccgtcagc
      721 cctgacggag gaagcgctga aacggatcgg cgaactgtac gccatcgagg cagagataag
      781 gggaatgacg gcggaacagc gccttgccga acgtcagttg aaaacgaaac cgctgctgaa
      841 atccctggaa agctggctgc gtgaaaagat gaaaaccctg tcgcgacact cagaactggc
      901 gaaagcgttc gcata
//
LOCUS       DAJTXQ010000337          911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000337 DAJTXQ010000000
VERSION     DAJTXQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.343"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            345..>911
                     /gene="ypdA"
                     /locus_tag="OO515_004364"
     CDS             345..>911
                     /gene="ypdA"
                     /locus_tag="OO515_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6578102.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVL"
BASE COUNT          209 a          224 c          201 g          277 t
ORIGIN      
        1 gtccgagcga cgctgccaca atggtggctg ttttgaaaat agcctgatta atttctgtct
       61 gtccagcgtt ttagaacatt tttatccatg gctttaaatg aggaactcca cttcaaaaac
      121 aggaaatttt tctacgctaa caagaaaatt accaccaatt ttattaacat tataatttca
      181 ataaatttac cagccagaag tgcattatca agagatacat tgctaataca ccatttatct
      241 ttcaatcccc ttttaaattt atggtttttc ctcaaaaacg gcctgctggt catcagcctt
      301 caggttacct atcatagagg tttaatcctt attcagagtc acccgtgcac gaaatattca
      361 acatgctgct ggcggtcttc gatcgggcag cgttaatgct tatctgcctg ttctttctca
      421 tccgtatccg cctgtttcgc gaactgttgc acaagtcggc gcactcccca aaagaattgc
      481 tcgccgttac cgccattttc tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg
      541 tagaaggctc gctggtgaac gtacgtatta tcgcggtgat gtccgacggg attctgtttg
      601 gcccgtgggt aggcatcatt accggcgtga ttgcgggtat tcaccggtat ttaattgata
      661 tcggcggcgt gacggcgatc ccctgcttta tcaccagcat tctggcgggt tgtatatcag
      721 gctggataaa cctgaaaatc cccaaagcac agcgctggcg cgtcagtatt ctcggcggca
      781 tgttgtgtga gacgttgacc atgattctgg taattgtctg ggcaccaact accgcgttgg
      841 ggatcgatat cgtctctaaa atcggcattc caatgatcct cggtagcgtc tgtatcggct
      901 ttattgtgct t
//
LOCUS       DAJTXQ010000338          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000338 DAJTXQ010000000
VERSION     DAJTXQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.344"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(23..>909)
                     /locus_tag="OO515_004365"
     CDS             complement(23..>909)
                     /locus_tag="OO515_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6578103.1"
                     /translation="LTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFYAVQ
                     QLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNKESR
                     CSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTETLI
                     QLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEAQII
                     IKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDMLIF
                     GEPVTRIYPAGDMEEVDA"
BASE COUNT          250 a          220 c          156 g          283 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcagga
//
LOCUS       DAJTXQ010000339          906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000339 DAJTXQ010000000
VERSION     DAJTXQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.345"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO515_004366"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OO515_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6578104.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAJTXQ010000340          903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000340 DAJTXQ010000000
VERSION     DAJTXQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.346"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..512)
                     /locus_tag="OO515_004367"
     CDS             complement(<1..512)
                     /locus_tag="OO515_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6578105.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(758..>903)
                     /locus_tag="OO515_004368"
     CDS             complement(758..>903)
                     /locus_tag="OO515_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6578106.1"
                     /translation="ESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYK
                     PQH"
BASE COUNT          253 a          202 c          216 g          232 t
ORIGIN      
        1 gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag tgcaaacagt
       61 tcagcgtcga tgatttgatc aacttgttgc agttcagcga aatgattgcc gccaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cgcagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gccaacagag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat atatttgccc atgtaccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgctgc gactcgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatattgca
      781 tataaaaatt agtaaaccac gctccccggc attcaacgtg ctgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc atctaccgta agttcggatt
      901 cag
//
LOCUS       DAJTXQ010000341          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000341 DAJTXQ010000000
VERSION     DAJTXQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.347"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(10..516)
                     /locus_tag="OO515_004369"
     CDS             complement(10..516)
                     /locus_tag="OO515_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578107.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
     gene            complement(520..>902)
                     /locus_tag="OO515_004370"
     CDS             complement(520..>902)
                     /locus_tag="OO515_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578108.1"
                     /translation="DNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKP
                     TAFKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQR
                     LNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          194 a          251 c          228 g          229 t
ORIGIN      
        1 gtctgttcct taaatcttcc aggaaatttc tgcattgccg tcagcatcac cggcacctgt
       61 gaattcagcg ttggtcagca ccacgttttt gccactgact gacgtggaca tgaatccacc
      121 cagcggcact ttgatggatt catcagtgga gacgacaacg tataccggat cgcctttttt
      181 gatagtgctg gcatcaaaat cagaaccgag attaacggtc acgtagccac gcttcatggc
      241 gtcgcccggg aagttcttgc ctgttcccac ctggcgaacc atgtccggct gcgacgtggt
      301 cggataaggg cgcacgtaga tccccttcac cttgtctgcg gtatcaccat ctgccagcgg
      361 cacgaaaaaa ccgtcatcat cgtatttacc agccaggcca taggcagcga aggcgttatc
      421 ggatttaagg accaccggtt cgacggttaa gtcctgcggg cgagagacag ccccggcaat
      481 gccaacaggc atccggtaca gaaatacatt attcattttt taccctttac ggtttgccca
      541 gaattcagcg ttttgtttgt tcagggaagc gatactggtc atgcccatgt ttaggcgctg
      601 tgcatcgccg gtggtggcgc gggtgtttcg ccctttggca acctcagaca cggcattaaa
      661 cgccatgtcg accgattgtt tcggtaattt gcggatatcc gcatcaccga ctatctggcg
      721 aaccagcgtt ttgtcagcag aagccagaac ctcgcgtttg aacgcggtcg gtttcatctt
      781 acggctcaga tcgatacccg gaacgataac ttcggcacgc caggctgagt caccagtaat
      841 cgtggtttcc tcttcatcgt cctcgccgtc accggtcgga ttatcgtcag gcttattatc
      901 gt
//
LOCUS       DAJTXQ010000342          897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000342 DAJTXQ010000000
VERSION     DAJTXQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.348"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..266
                     /locus_tag="OO515_004371"
     CDS             <1..266
                     /locus_tag="OO515_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6578109.1"
                     /translation="GELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAVLVAS
                     QLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(267..500)
                     /locus_tag="OO515_004372"
     CDS             complement(267..500)
                     /locus_tag="OO515_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6578110.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(586..759)
                     /gene="ortT"
                     /locus_tag="OO515_004373"
     CDS             complement(586..759)
                     /gene="ortT"
                     /locus_tag="OO515_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6578111.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
BASE COUNT          223 a          213 c          221 g          240 t
ORIGIN      
        1 ttggcgaact ggtgtccagc gtgcgctggt ggacacttat acccggcttg ctgggatcgt
       61 tttttgtctt cgccagtatc agcggatatc agaatgtggg agcggcgaca actattgcag
      121 tactggtcgc cagccagcta attggcggtc tgatgctgga tatcttccgc agtcatggcg
      181 taccactacg ggcgttgttc ggcccaattt gtggcgcaat tttacttgtg gttggcgcct
      241 ggttggtagc cagacgctca ttttaatcag aggatggtgc cgcctttggt taactgttcg
      301 tggcgcgctt ccatatcttc tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa
      361 cgctgctgct gggtgtagcg atcttcccga cttaactccg cgtcatcgct cagggcgatt
      421 aataattcgt tcatatgggc aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg
      481 acttcatcca gatgcgacat actttctcct taaccacttg tgaaatttat cgattcatcc
      541 aaatgatata agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa
      601 caacagcgcc accgggaaac tcatcggcca tgttgccccc accagaaagg cgcttaagaa
      661 acggatgcgt ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga ttgctgccat
      721 aaccgcataa aaaacaagca tatgttgata gagagacatg tacgggcgtc cagccagatg
      781 aataaaacgc gcgcaaatat gcgtcatttt ttgacgtgca tcaagttttt tcaccagttt
      841 gagaaaaaat catcaaacag caacttgccc ttatccatct tcagaatgag tattatt
//
LOCUS       DAJTXQ010000343          895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000343 DAJTXQ010000000
VERSION     DAJTXQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.349"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..220
                     /locus_tag="OO515_004374"
     CDS             <1..220
                     /locus_tag="OO515_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="catalyzes the dehydration of L-carnitinyl-CoA to
                     crotonobetainyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carnitinyl-CoA dehydratase"
                     /protein_id="HCR6578112.1"
                     /translation="RELAQQLVNSAPLAIAALKEIFRTTSEMPVEEAYRYIRSGVLKH
                     YPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            226..816
                     /gene="caiE"
                     /locus_tag="OO515_004375"
     CDS             226..816
                     /gene="caiE"
                     /locus_tag="OO515_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6578113.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
BASE COUNT          197 a          221 c          283 g          194 t
ORIGIN      
        1 ccgcgaactg gcgcagcagc tggttaacag cgccccgctg gcgattgcgg cgctgaaaga
       61 gatcttccgc accaccagcg aaatgccggt agaagaagcg tatcgctata ttcgcagcgg
      121 cgtgttgaaa cactatccgt cggttctgca ttcggaggat gccattgaag ggccgctggc
      181 gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag ctattacgcc
      241 tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag tgccgtcttg
      301 attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc actgcgtggt
      361 gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg cattatgcat
      421 ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca cggagcgatc
      481 ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt gattatggat
      541 ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa agcaggcttt
      601 cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg cagtgttagt
      661 gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct tgtcgggcgc
      721 tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa tcgcccccgt
      781 ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg gcgtaaacgc
      841 cttatccaac tctgctggag agcaaccgta ggccggataa gatgcgccag catcg
//
LOCUS       DAJTXQ010000344          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000344 DAJTXQ010000000
VERSION     DAJTXQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.350"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            69..869
                     /gene="tcyJ"
                     /locus_tag="OO515_004376"
     CDS             69..869
                     /gene="tcyJ"
                     /locus_tag="OO515_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6578114.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          248 a          179 c          250 g          207 t
ORIGIN      
        1 gagttgtttt tgtgtttaca aacaatggct ctacactgca aacagacata acaacattcg
       61 gggtgaatat gaaattagca catctgggac gtcaggcatt gatgggtgtg atggccgtgg
      121 cgctggttgc gggtatgagc gttaaaagtt ttgcagatga aggtctgctt aataaagtca
      181 aagagcgcgg cacgctgctg gtagggctgg aaggaactta tccgccgttc agttttcagg
      241 gggatgacgg caaattaacc ggttttgaag tggaatttgc ccaacagctg gcaaaacatc
      301 ttggcgttga ggcgtcacta aaaccgacca aatgggacgg tatgctggcg tcgctggact
      361 ctaaacgcat tgatgtggtg attaaccagg tcaccatttc tgacgagcgc aagaaaaaat
      421 acgatttttc taccccttac accatttccg gtattcaggc gctggtgaaa aaaggtaacg
      481 aaggcaccat taaaacagcc gatgatctga aaggcaaaaa agtgggtgtc ggtctgggca
      541 ccaactatga agagtggctg cggcagaatg ttcagggtgt cgatgtgcgt acctatgatg
      601 atgacccgac caaatatcag gatctgcgcg taggccgtat cgatgcgatc ctcgttgacc
      661 gtctggcggc gctggatctg gtgaagaaaa ccaacgatac gctggcagta accggtgaag
      721 cattttcccg tcaggagtct ggcgtggcgc tgcgtaaagg aaatgaagat ctgctgaaag
      781 cagtgaatga tgcaattgcg gaaatgcaaa aagatggcac tctgcaagcc ctttccgaaa
      841 aatggtttgg tgctgatgtg accaaataat cagcataatg acaa
//
LOCUS       DAJTXQ010000345          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000345 DAJTXQ010000000
VERSION     DAJTXQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.351"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..689)
                     /gene="hyaA"
                     /locus_tag="OO515_004377"
     CDS             complement(<1..689)
                     /gene="hyaA"
                     /locus_tag="OO515_004377"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6578115.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQR"
BASE COUNT          194 a          238 c          240 g          191 t
ORIGIN      
        1 cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa gcgatcaaag
       61 gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca gccaggtact
      121 ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt cggattgggc
      181 cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat cgcgctggct
      241 ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat acagaacatc
      301 ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc attgtattgc
      361 gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat caaagtatcg
      421 tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg agcggagcgg
      481 ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac cggaatgcgg
      541 ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa tcccagcgac
      601 gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg acgccgcatg
      661 gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg cgacaccggc
      721 aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa accatcgctt
      781 cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc gccagaaagt
      841 attgttaata aagcgtagtg aaa
//
LOCUS       DAJTXQ010000346          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000346 DAJTXQ010000000
VERSION     DAJTXQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.352"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..130
                     /locus_tag="OO515_004378"
     tRNA            54..130
                     /locus_tag="OO515_004378"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OO515_004379"
     tRNA            173..248
                     /locus_tag="OO515_004379"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="OO515_004380"
     rRNA            432..>842
                     /locus_tag="OO515_004380"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAJTXQ010000347          833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000347 DAJTXQ010000000
VERSION     DAJTXQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.353"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>833
                     /locus_tag="OO515_004381"
     CDS             <1..>833
                     /locus_tag="OO515_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6578116.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     L"
BASE COUNT          189 a          267 c          254 g          123 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gttatccgca cggatgatga
      361 gcgcacccac cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca
      421 atatgcagag ccgctggtcg aaagtcgcta tctttacgac ccgctgggcc gcagggtggc
      481 aaaacgggta tggcggcgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc
      541 gcaagtgacc tggtacggct gggacggcga ccggctgacc acaatacaga acgacagaac
      601 ccgcatccag acgatttatc agccggggag cttcacgcca ctcatcaggg tcgaaactgc
      661 caccggtgag ctggcgaaaa cgcagcgccg cagcctggcg gatgcccttc agcagtccgg
      721 cggcgaagac ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct
      781 ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc tgg
//
LOCUS       DAJTXQ010000348          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000348 DAJTXQ010000000
VERSION     DAJTXQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.354"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..516)
                     /locus_tag="OO515_004382"
     CDS             complement(<1..516)
                     /locus_tag="OO515_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6578117.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          267 a          173 c          152 g          233 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagc
//
LOCUS       DAJTXQ010000349          790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000349 DAJTXQ010000000
VERSION     DAJTXQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.355"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>790
                     /locus_tag="OO515_004383"
     CDS             <1..>790
                     /locus_tag="OO515_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6578118.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAJTXQ010000350          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000350 DAJTXQ010000000
VERSION     DAJTXQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.356"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..353)
                     /locus_tag="OO515_004384"
     CDS             complement(<1..353)
                     /locus_tag="OO515_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6578119.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVT"
     gene            complement(400..606)
                     /locus_tag="OO515_004385"
     CDS             complement(400..606)
                     /locus_tag="OO515_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578120.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT          218 a          196 c          153 g          209 t
ORIGIN      
        1 gtcacctgcc cgtcaataca gccctgcatt tcaccgaact gcggatggat cagcgtcccc
       61 ggcccggcgg tttcaatcgc tgtaatcagc ctgtcgcgct gctctgcgta atcatcacca
      121 acgagatacg cattaatcgt cagccgtcgc gtggcacgcc cgagatcttc cgtgtacggt
      181 ttgtcacggt ttgggtattc atggacctga acgcggcgtc cgaacgaacc ctcgtcgctc
      241 tccaccgaaa acggaacgcc acgaaatgaa gcgtcgtaaa gattatcgcg ccaattgata
      301 cctgaagatg cagaaaagag tgaagaaaga gaaggtaaag aaggtaagtc catactgtct
      361 ccactataaa aaaaccgcca atttctggcg gcttatatat taatcatcat taatatcttt
      421 catattcaat ttgcaatttt cgtaggtcac tcccgactcg ccattaacat taactccagc
      481 tctgatattc ccgttttctt ttacatctac aaaaaactgg cctcttggca tcttgaacgc
      541 aaatgtaaaa ccaacagtat ctgaatcaaa atcatcatct ttatatattg ggatcaaaaa
      601 tgtcatcgtc gctggtgatg cattgtttgc aaaatcaaat acacctatac ttttgccgaa
      661 ttcataagaa gcttttctca atataatcgc accgctggca gaaggggatg ggcacgatat
      721 cctcagttgt ctgaatccat ctgtatccaa gtcaacgtca ttatctgcag atgttt
//
LOCUS       DAJTXQ010000351          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000351 DAJTXQ010000000
VERSION     DAJTXQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.357"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..344
                     /locus_tag="OO515_004386"
     CDS             <1..344
                     /locus_tag="OO515_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6578121.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="OO515_004387"
     CDS             complement(406..>776)
                     /locus_tag="OO515_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6578122.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAJTXQ010000352          773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000352 DAJTXQ010000000
VERSION     DAJTXQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.358"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(158..>773)
                     /locus_tag="OO515_004388"
     CDS             complement(158..>773)
                     /locus_tag="OO515_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCR6578123.1"
                     /translation="SEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGKTDR
                     SANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDFV
                     CGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGE
                     ESIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT"
BASE COUNT          172 a          213 c          189 g          199 t
ORIGIN      
        1 agcgggcatg attcattgtc cttttacagc ctgttttctg ctcaaattat aagcttgaac
       61 taaggtaatg caaatgcatt attaatggac gtgggggctt taaacaataa aaacatggca
      121 ctaaggccat gttttgcgag ggacgttcca aaagaaatca ggtcaccggt gccgggttaa
      181 acaccgccag ctggttatgc agcccccatt gatccgagaa ggttttcttc ttcccgctgg
      241 cgacgtcgag aataaagtgg aacaacttcc agcccacctc ttcaatgctc tcttcgccgg
      301 tagcgatagt gcccgcgtta atatccatta aatcaaacca gcggttcgcc agctcggtgc
      361 gggttgccat tttaattacg ggtaccgcca tcaggccgta cggcgtacca cggccggtcg
      421 taaacacttg tacggtgata cccgaagcca cctgttgcgt gccacagaca aaatcgctgg
      481 ctggcgtcgc ggcgtaaatt aatccgcgtt tagtcgggcg ttgaccgggc gacagcactt
      541 caacaattgc acttttaccc gatttagcaa tggagccgag tgccttctcc accacatttg
      601 ccagaccgcc tttcttgttg cccggcgaag ggttggcgct gcggtcggtt tttcccatat
      661 tgagatagtt atcgtaccac tccatctcct ccagcagccg tttgccgacc tcttcgttca
      721 ctgcgcgtgg tgtcagcaga tggatcgcgt cacgcacttc cgttacttct gaa
//
LOCUS       DAJTXQ010000353          773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000353 DAJTXQ010000000
VERSION     DAJTXQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.359"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..>773)
                     /gene="rlmB"
                     /locus_tag="OO515_004389"
     CDS             complement(73..>773)
                     /gene="rlmB"
                     /locus_tag="OO515_004389"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6578124.1"
                     /translation="QALLERAPERFQEVFILKGREDKRLLPLIHALESQGVVIQLANR
                     QYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDGVTDPHNLGACL
                     RSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLARTMRMLQEENIWI
                     VGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSV
                     ATGICLFEAVRQRS"
BASE COUNT          181 a          223 c          195 g          174 t
ORIGIN      
        1 ccgtaattat cttaccagct atagacaaaa aaaaccatcc aaatctggat ggcttttcat
       61 aattcagacg aactagctgc gctgacgaac cgcttcaaat aagcaaattc cggttgcaac
      121 cgaaacgttc agggaagaaa cacttcctgc cattgggatg ctgatcaact catcgcaatg
      181 ttcacgggtc aggcgacgca taccttcacc ttccgcgccc atcaccagcg ccaagcgtcc
      241 ggtcattttg ctctgataga gtgtatgatc cgcctcgcct gccgtaccga cgatccagat
      301 attctcttcc tgcaacatac gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac
      361 gctttctgcc gcgccgcagg ctactttttt cgccgtagcg ttgagctgtg cggagcgatc
      421 tttcggcaca atcaccgcgt gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag
      481 gttgtgcgga tcggttacac cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat
      541 cagatccggc agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc
      601 ctgatgcacg gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac
      661 cacgccctga gactcaaggg cgtgaatcag cggtaacaga cgtttatctt cacggccttt
      721 taaaataaag acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gca
//
LOCUS       DAJTXQ010000354          768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000354 DAJTXQ010000000
VERSION     DAJTXQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.360"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            124..369
                     /gene="tnpA"
                     /locus_tag="OO515_004390"
                     /pseudo
     CDS             124..369
                     /gene="tnpA"
                     /locus_tag="OO515_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          245 a          172 c          137 g          214 t
ORIGIN      
        1 atgcatgttc acctggtctt tgtcaccaaa taccgacgtc agatttttga ccatgacgcg
       61 acagaaaaac tacgcactta cttttcaaat gtatgtgctg attttgaagc tgaactggtt
      121 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg
      181 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggtgactgcg acgagatcga
      241 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtgttgg ctattttgcc
      301 agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
      361 cctggttagg tggaaaacca cgccttatat ccctgccctg aaggacgggg ttttacggcg
      421 caccggataa gcctggagat tatctacacc agcatggaca tcattgtact atgccccgta
      481 catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat tatgcgtctc
      541 agtttgtctc atctatctta cgcaaaaaaa aatagaacta atatcaagcg gcgctatatt
      601 gcgtattaaa taaaaccacc tcaattacat taataaatct aaacaaacat catgcaaata
      661 atttccatgg ttatttctag gctatttaaa ttaaataaat aaattatgag tcaatcataa
      721 ataacaacct ttaatataat ttaactacca aatttatatt cgttcaca
//
LOCUS       DAJTXQ010000355          729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000355 DAJTXQ010000000
VERSION     DAJTXQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.361"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..250
                     /locus_tag="OO515_004391"
     CDS             <1..250
                     /locus_tag="OO515_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6578125.1"
                     /translation="NDNAQTLANLSNALFDAGVMPYYLHVLDKVQGAAHFMVSDDEAR
                     QIMRELLTLVSGYLVPKLAREIGGEPSKTPLDLQLRQQ"
     gene            640..>729
                     /locus_tag="OO515_004392"
     CDS             640..>729
                     /locus_tag="OO515_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578126.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT          216 a          145 c          164 g          204 t
ORIGIN      
        1 gaacgataac gcacaaacgt tggcaaacct gagtaatgcg ttgttcgatg ccggcgtgat
       61 gccgtattac ctgcatgtgc tagataaagt acagggcgcg gcgcatttta tggtgagtga
      121 tgacgaagca cggcagatta tgcgtgagtt gctgacattg gtgtcgggat atctggtgcc
      181 gaaactggcg cgagaaattg gcggcgaacc cagcaaaacg ccgctggatc tccagctacg
      241 ccagcagtaa ctgattaaac agggaagtta aacattagcc tgatgcgcta cgcttatcag
      301 gcctacattg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt
      361 ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaactggga
      421 aatttaatct ttgcccgttg cataaccttt aattattcat atacattgct gttgttactc
      481 tttatatagc caacaccaat aattaacctt tcttttctgt ttatatagag tgagtacggc
      541 tatttatgtt attttaacaa ctattactac caagcccatt tatctggctt ataaattaaa
      601 attaattatt taattgagaa atggttaggg agaacctaca tggccataag catcaagggt
      661 gttaataccg gtgttattcg caagagtaat aatttcattg cactggcgct gaaaattaaa
      721 gaacctcgc
//
LOCUS       DAJTXQ010000356          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000356 DAJTXQ010000000
VERSION     DAJTXQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.362"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..726
                     /locus_tag="OO515_004393"
                     /pseudo
     CDS             35..726
                     /locus_tag="OO515_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJTXQ010000357          716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000357 DAJTXQ010000000
VERSION     DAJTXQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.363"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>716
                     /locus_tag="OO515_004394"
     CDS             <1..>716
                     /locus_tag="OO515_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6578127.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGG"
BASE COUNT          168 a          171 c          212 g          165 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcgg
//
LOCUS       DAJTXQ010000358          715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000358 DAJTXQ010000000
VERSION     DAJTXQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.364"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>715
                     /locus_tag="OO515_004395"
     CDS             <1..>715
                     /locus_tag="OO515_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6578128.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAEL"
BASE COUNT          137 a          206 c          208 g          164 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgt
//
LOCUS       DAJTXQ010000359          713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000359 DAJTXQ010000000
VERSION     DAJTXQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.365"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            37..>713
                     /locus_tag="OO515_004396"
     CDS             37..>713
                     /locus_tag="OO515_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6578129.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDE"
BASE COUNT          205 a          148 c          163 g          197 t
ORIGIN      
        1 ttcgctagta aactgataat cagacaaaat ggtgacatga gggacattgt ggaccctgta
       61 ttctctatcg gtatctcatc attatgggat gagctgcgac atatgccagc aggcggcgtc
      121 tggtggttta acgtcgatcg ccatgaagat gctatcagtc tggcgaatca aacaattgca
      181 tcccaggctg aaaccgcaca cgtcgcggtc attagcatgg acagcgatcc ggcgaaaatc
      241 tttcaattag atgattctca agggccggaa aaaataaaat tattttcaat gctaaatcat
      301 gaaaaaggtc tatactattt ggcccgtgat ttgcagtgtt ctattgatcc ccataattac
      361 ctttttattc ttgtttgcgc aaataacgca tggcaaaaca ttcctgccga gcggcttcgc
      421 tcatggttgg ataaaatgaa taaatggagc aggttaaacc attgttcgct tttggtaatt
      481 aatcccggaa ataataacga taaacaattt tcattgttgc ttgaggaata ccgttcactt
      541 tttggtcttg ccagtttgcg ttttcagggt gaccaacatt tgctggatat tgccttctgg
      601 tgcaacgaaa aaggggtcag cgcccgtcag cagcttagcg ttcagcaaca aaatggtatc
      661 tggacattag ttcaaagcga agaggcggag atccaaccac gcagcgacga aaa
//
LOCUS       DAJTXQ010000360          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000360 DAJTXQ010000000
VERSION     DAJTXQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.366"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAJTXQ010000361          701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000361 DAJTXQ010000000
VERSION     DAJTXQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.367"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>701
                     /gene="pgl"
                     /locus_tag="OO515_004397"
     CDS             <1..>701
                     /gene="pgl"
                     /locus_tag="OO515_004397"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6578130.1"
                     /translation="MVVSPDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSP
                     THISTDHQGQFVFVGSYNAGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLW
                     VPALKQDRICLFTVSDDGHLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSV
                     DVWELKDPHGNIECVQTLDMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSV
                     SEDGSVLSKEGFQPT"
BASE COUNT          145 a          178 c          205 g          173 t
ORIGIN      
        1 cgatggtggt cagcccggac aaacgttatc tctatgttgg tgttcgccct gagtttcgcg
       61 tcctggcgta tcgtattgcc ccggacgatg gcgcactgac ctttgccgca gagtctgcgc
      121 tgccgggtag tccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtaggtt
      181 cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg
      241 tcgatgtggt cgaggggctg gacggttgcc attccgccaa tatctcaccg gacaaccgta
      301 cgctgtgggt tccggcatta aagcaggatc gcatttgcct gtttacggtc agcgatgatg
      361 gtcatctggt ggcgcaggac cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc
      421 atatggtatt ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag
      481 tggatgtctg ggaactgaaa gatccacacg gcaatattga atgtgtccag acgctggata
      541 tgatgccgga aaacttctcc gacacctgtt gggcggctga tattcatatc accccggatg
      601 gtcgtcattt atacgcctgc gaccgtaccg ccagtctgat taccgttttc agcgtttcgg
      661 aagatggcag cgtgttgagt aaagaaggct tccagccaac g
//
LOCUS       DAJTXQ010000362          700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000362 DAJTXQ010000000
VERSION     DAJTXQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.368"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..302
                     /locus_tag="OO515_004398"
     CDS             <1..302
                     /locus_tag="OO515_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6578131.1"
                     /translation="VSKAGERYRRDVALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRD
                     LDNILKAPLDALTHAGVLMDDEQFDEINIVRGQPVSGGRLGVKIYPIMHEEQVKK"
     gene            299..>700
                     /locus_tag="OO515_004399"
     CDS             299..>700
                     /locus_tag="OO515_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6578132.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          193 a          147 c          199 g          161 t
ORIGIN      
        1 ttgtatcaaa agccggtgag cgttatcgcc gggatgtggc gcttattgtc cgtcagcagc
       61 gactgaaatt aagcctgtcc ggaagactgg cgataaagat tattgcagag ccaccggata
      121 agcgccgtcg tgacctggac aatatcctga aagcaccact ggatgcactg acacatgcgg
      181 gagtgttaat ggacgatgag cagtttgatg aaatcaatat cgttcgtggt cagccagtat
      241 ctggtggacg tctgggggtg aagatttacc ccataatgca tgaagagcag gtcaaaaaat
      301 gaaactggaa gatttaccga aatactactc cccaaaatcc ccttgcctga ccgatgcatc
      361 ggcctcaacg tcaaaagatg cgctgagtat cactgatgtg atggccgcgc agggcatgac
      421 acagaatcgg gctgagatgg ggttttctgc gttcctgggg aaaatgggca tcagtatgaa
      481 tgacagggcg cgggcaacag aattactggc agattatgca ctcagtcggt gcgatcgtgt
      541 ggcggcgttg agaaaacttc cggcagaaat aaaaccggta gtgatgcgca ttatggcttc
      601 gtacgctttt gaggattatg cccgcagcgc agcgagtaaa aagcagtgcc cttgttgcta
      661 tggggaaaaa tttattgaaa gcgtagtttt tacaaacaag
//
LOCUS       DAJTXQ010000363          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000363 DAJTXQ010000000
VERSION     DAJTXQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.369"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..194
                     /gene="csgB"
                     /locus_tag="OO515_004400"
     CDS             <1..194
                     /gene="csgB"
                     /locus_tag="OO515_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6578133.1"
                     /translation="NLAYIDQAGSANDASISQGAYGNTAMIIQKGSGNKANITQYGTQ
                     KTAIVVQRQSQMAIRVTQR"
     gene            235..687
                     /gene="csgA"
                     /locus_tag="OO515_004401"
     CDS             235..687
                     /gene="csgA"
                     /locus_tag="OO515_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6578134.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          187 a          154 c          173 g          183 t
ORIGIN      
        1 ataaccttgc atatattgat caggcgggca gtgccaacga tgccagtatt tcgcaaggtg
       61 cttatggtaa tactgcgatg attatccaga aaggttctgg taataaagca aatattacac
      121 agtatggtac tcaaaaaacg gcaattgtag tgcagagaca gtcgcaaatg gctattcgcg
      181 tgacacaacg ttaatttcca ttcgactttt aaatcaatcc gatgggggtt ttacatgaaa
      241 cttttaaaag tagcagcaat tgcagcaatc gtattctccg gtagcgctct ggcaggtgtt
      301 gttcctcagt acggcggcgg taaccacggt ggtggcggta ataatagcgg cccaaattct
      361 gagctgaaca tttaccagta cggtggcggt aactctgcac ttgttctgca aactgatgcc
      421 cgtaactctg acttgactat tacccagcat ggcggcggta atggtgcaga tgttggtcag
      481 ggctcggatg acagctcaat cgatctgacc caacgtggct tcggtaacag cgctactctt
      541 gatcagtgga acggcaaaaa ttctgaaatg acggttaaac agtttggtgg tggcaacggt
      601 gctgcagttg accagactgc atctaactcc tccgtcaacg tgactcaggt tggctttggt
      661 aacaacgcga ccgctcatca gtactaatac atcattt
//
LOCUS       DAJTXQ010000364          677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000364 DAJTXQ010000000
VERSION     DAJTXQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.370"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..361
                     /locus_tag="OO515_004402"
                     /pseudo
     CDS             1..361
                     /locus_tag="OO515_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAJTXQ010000365          676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000365 DAJTXQ010000000
VERSION     DAJTXQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.371"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            32..>676
                     /gene="mpaA"
                     /locus_tag="OO515_004403"
     CDS             32..>676
                     /gene="mpaA"
                     /locus_tag="OO515_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6578135.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPIS"
BASE COUNT          143 a          191 c          182 g          160 t
ORIGIN      
        1 gcctgtaata cttcctgaac cagagacgat catgaccgta acccgcccac gcgccgaacg
       61 cggcgcattt ccgcccggaa cagaacatta cggacgttca ttattgggcg cgccgttaat
      121 ctggtttcct gcccctgcag ccagccgcga aagtggtttg attctggctg gcactcacgg
      181 tgatgaaaac tcttcggtcg tcacgctctc ctgtgctctg cggacgttga caccttcttt
      241 gcgccgccat catgtggtgc tgtgtgtgaa tcctgacggc tgccagctgg ggttacgggc
      301 caatgctaat ggtgtggatt taaacagaaa ctttccggcg gcgaactgga aggaaggtga
      361 aacggtttat cgctggaaca gcgccgctga agaacgtgat gtcgtattac tgacaggtga
      421 caaacctggc tcagaacctg aaacccaggc gctgtgccag cttatacacc gcattcaacc
      481 tgcctgggtg gtctcattcc acgatccact ggcctgtatt gaagatccca gacacagcga
      541 attaggcgaa tggctggccc aggcgtttga attgcctctg gtaaccagcg tcggttatga
      601 aacgcctggc tctttcggca gttggtgtgc agatctcaac ttgcattgca tcaccgcaga
      661 gtttccgccc atctcc
//
LOCUS       DAJTXQ010000366          666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000366 DAJTXQ010000000
VERSION     DAJTXQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.372"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..660
                     /locus_tag="OO515_004404"
     CDS             <1..660
                     /locus_tag="OO515_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6578136.1"
                     /translation="ERDEARDLLHSHGTGKGRAFCEKLAQQAGIDQSHPALQRWYASI
                     HETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMIAVSPLALVDMAFIAWRNL
                     RLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVREVGMDWMSQDLAARLSTR
                     AAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRRQLIGQVKETLQKGKTPSE
                     K"
BASE COUNT          150 a          167 c          199 g          150 t
ORIGIN      
        1 gaacgcgacg aagcgcgtga tttattgcat agccacggca cgggcaaagg ccgcgcattt
       61 tgcgaaaaac tggcgcagca ggcgggtatt gatcagtcgc atccggcgct gcaacgctgg
      121 tatgcctcaa tccatgaaac gcaaaacgac cgtgaagtgg tcagcctgta tgcgcatttg
      181 gtccagccag ttttagatgc ccaggcgcgg cgcgaaatca gccgttcggc ggcggaatca
      241 acgttgatga ttgcggtcag cccgctggcg ttggtcgata tggcgtttat cgcctggcgc
      301 aatctgcgtt taattaatcg catcgccacg ctgtatggca ttgaactggg gtattacagc
      361 cgtttgcgtc tgtttaagct ggtattgctg aatatcgctt ttgccggagc cagcgaactg
      421 gtgcgcgaag tggggatgga ctggatgtcg caagatctcg ctgctcgttt gtctacccgc
      481 gcagctcagg ggattggtgc aggacttctg acggcacgac tcgggattaa agctatggag
      541 ctttgccgcc cgctgccgtg gattgacgat gacaaacctc gcctcgggga tttccgtcgt
      601 cagcttatcg gtcaggtgaa agaaacactg caaaaaggca aaacacccag cgaaaaataa
      661 tgcaat
//
LOCUS       DAJTXQ010000367          658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000367 DAJTXQ010000000
VERSION     DAJTXQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.373"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            502..>658
                     /gene="csgB"
                     /locus_tag="OO515_004405"
     CDS             502..>658
                     /gene="csgB"
                     /locus_tag="OO515_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6578137.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQA"
BASE COUNT          226 a          103 c          131 g          198 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctg
//
LOCUS       DAJTXQ010000368          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000368 DAJTXQ010000000
VERSION     DAJTXQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.374"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..60)
                     /locus_tag="OO515_004406"
     CDS             complement(<1..60)
                     /locus_tag="OO515_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6578138.1"
                     /translation="MRRWISQNNIRLPRGAFFIS"
     gene            complement(35..223)
                     /locus_tag="OO515_004407"
     CDS             complement(35..223)
                     /locus_tag="OO515_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6578139.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(234..446)
                     /gene="cspG"
                     /locus_tag="OO515_004408"
     CDS             complement(234..446)
                     /gene="cspG"
                     /locus_tag="OO515_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6578140.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
BASE COUNT          191 a          136 c          146 g          182 t
ORIGIN      
        1 agatataaaa aaggctccac gagggagcct gatattattt tgtgaaatcc agcggcgcat
       61 ggtcagtgcg tatttgcagc gcgacgttat caaaggtgat catcgtcgat ttacaaaaaa
      121 tacacttagc gcccaaaggg tttcgctccg tcacatcgaa tgccgatgtg cggtactgtg
      181 aaccatgaca acatggacag cggaaatgga gatgttgagt aataacggca accttagagc
      241 gtaacaacgt tcgctgccgc ggggccacgt tgcccctgct caatagaaaa ttcaactttc
      301 tgattttcgt tcagcgtgcg gaattcattg ctctggatgg cggtgaaatg gacgaaaacg
      361 tctttgctgc catcatcagg agtgataaag ccaaaacctt tatctgcgtt aaaccatttt
      421 actaaaccag tcattttatt agacatattt tacttccttc attttgggcc tttcggcaaa
      481 cagggcttta atacagaaac tacttagtgt tcaggagaga gactcaaaag aaggggataa
      541 tgcctgataa tgagaactgc tttagtaaac tactttgtat gctgtctgtc tttcaaaccg
      601 acgcagctat taacgcatga taattgagat taagcaaatt ttattttagc cgtcc
//
LOCUS       DAJTXQ010000369          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000369 DAJTXQ010000000
VERSION     DAJTXQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.375"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>655
                     /locus_tag="OO515_004409"
     CDS             <1..>655
                     /locus_tag="OO515_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6578141.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     "
BASE COUNT          130 a          192 c          187 g          146 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccg
//
LOCUS       DAJTXQ010000370          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000370 DAJTXQ010000000
VERSION     DAJTXQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.376"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>642
                     /gene="fliD"
                     /locus_tag="OO515_004410"
     CDS             <1..>642
                     /gene="fliD"
                     /locus_tag="OO515_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6578142.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          218 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac aa
//
LOCUS       DAJTXQ010000371          639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000371 DAJTXQ010000000
VERSION     DAJTXQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.377"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(387..638)
                     /locus_tag="OO515_004411"
     CDS             complement(387..638)
                     /locus_tag="OO515_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6578143.1"
                     /translation="MRKAGNSPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDK
                     VFCPTRGGNKMAFVDKDGVEVESSTHWMPLPEPPQEVNQ"
BASE COUNT          161 a          184 c          139 g          155 t
ORIGIN      
        1 cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt tctctgtctg
       61 ccttacagtg acatacccga ggcagacagc ctcaagcacc cgcagcaatc ttaccgacac
      121 cgataagaaa acgctatttt ttattgccag caccatctat ccaggctagt ttatctgcgt
      181 cagaacggcg caatgcgctc gcctgagata cgtttacttg ccatggtaac aacaggtaac
      241 ggcaattgct gtttctgttt tgtttccttc aaaaaccccg gaccatcaat ccggggtttt
      301 tgtttgttaa ccccaacggc aaatcgaata caccaccagc gccaccgcca tcgcaattcc
      361 taccgtggtg aatgcctcag gccaggtcat tgattcacct cctgcggtgg ttccggtagc
      421 ggcatccagt gagttgagct ctctacttca acgccgtctt tgtccacaaa agccatctta
      481 ttcccgccac gagtggggca aaaaacctta tcccaggaac cggggaatac attcccgaga
      541 tcatcgagaa gaatcacatc gcaatattca gcaggaactg catcactaca gcttatccag
      601 ccagccggag ctaccggaga gttgccagcc ttacgcatg
//
LOCUS       DAJTXQ010000372          636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000372 DAJTXQ010000000
VERSION     DAJTXQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.378"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>636
                     /locus_tag="OO515_004412"
     CDS             <1..>636
                     /locus_tag="OO515_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6578144.1"
                     /translation="LCVMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENL
                     GGELINLSLEDAIMATIPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTE
                     CSPEFNALNFEDVTYTDAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDR
                     MEAELRQNNTPPTDKIIPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
BASE COUNT          162 a          177 c          145 g          152 t
ORIGIN      
        1 tttatgcgta atgcacagct ccattcaaat tatggtggga tgtgcagggc agtcgcaaga
       61 ctggccgggt tctcgagtga ccggtatttc cacccctgta cgtctcacca cccttatggt
      121 cgtggaaaac ctcggtggtg agttaattaa cttatcactc gaggatgcca tcatggctac
      181 tatccctgcc ctttctcacc ctgacgtaac catcgaaaat ggtcgcgctg tcactacgtc
      241 tattgcgatc gcagagttct ttggcaagcg tcacgacaat gtgttacgtg ccatcgcaaa
      301 tactgaatgc tcacctgaat ttaacgccct caattttgag gacgtcacct acactgacgc
      361 caaaggcgaa aaacgcccga tgtaccaaat caccaaaaac ggcttcgttt tcctggtgat
      421 gggattcact ggcaaaaaag ccgctgcatt taaagaagcc tacattgctg agttcgatcg
      481 catggaggcc gaactgcgcc agaataacac cccacccact gacaaaataa ttccaggcga
      541 tggccgcacc ctggttgttc acttcgacga acgcggcaac atcagattca ccgaaaccgt
      601 tcctgatggc gcacgggtct gtaccatgga gacttt
//
LOCUS       DAJTXQ010000373          631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000373 DAJTXQ010000000
VERSION     DAJTXQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.379"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..437
                     /locus_tag="OO515_004413"
     CDS             <1..437
                     /locus_tag="OO515_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6578145.1"
                     /translation="CSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFI
                     PSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGS
                     AGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          186 a          126 c          134 g          185 t
ORIGIN      
        1 gttgcagtac caaagctgta gtaagtatta atcccataaa actagccacc aaagatgcgt
       61 tgcaacagtt taaagatgta ctgccatatc ttttgttagg agtattaata ggatctttta
      121 tttatggctt tattccctca gcgtggattg ccgctcatgc aggagcagat aatcccttcg
      181 ccatcccatt aagcgccgtt gtcggtattc cgctgtatat ccgggctgag gcggttatcc
      241 ctctggcatc tgttctgatg acaaaaggaa tgggtctggg agcattaatg gcattaatca
      301 tcggcagtgc cggcgcaagc ctgacggaag tgatattact taaatcaatg ttcagaatgc
      361 cgatgatagc tgcattcctg acggttatat taggtatggc tatcttgatg ggctatttga
      421 ctcaattgct attttaaata gatgccagca gtaaactata agatgcatag ttagtgccat
      481 atccttctaa gtagaaaatc atagacagga taatccttaa aaatcacaaa tgatttaact
      541 aatgcattga gaatgatgac ggctcggtca acaagtcatc aacagacaat aaccacgtaa
      601 tcatgcttct ctgacatttt atcggagaag c
//
LOCUS       DAJTXQ010000374          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000374 DAJTXQ010000000
VERSION     DAJTXQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.380"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            450..>617
                     /locus_tag="OO515_004414"
     CDS             450..>617
                     /locus_tag="OO515_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6578146.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          177 a          106 c          137 g          197 t
ORIGIN      
        1 tatgcaagaa cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa
       61 tcagccggag atgattttac ataattgcta cggaattatt caatacagga attgcttgcg
      121 tctgcatgga ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg
      181 taactggttg aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa
      241 atttatttaa cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcgt
      301 gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca
      361 accataacac acactctact gtctgagtcc aacgtttttt aacattcttg ttaaaattat
      421 gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa
      481 ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc
      541 agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag
      601 tgggagacgt tatcgat
//
LOCUS       DAJTXQ010000375          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000375 DAJTXQ010000000
VERSION     DAJTXQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.381"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="OO515_004415"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="OO515_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6578147.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT          167 a          126 c          159 g          165 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgc
//
LOCUS       DAJTXQ010000376          616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000376 DAJTXQ010000000
VERSION     DAJTXQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.382"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="OO515_004416"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="OO515_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6578148.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          137 a          165 c          175 g          139 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc
      601 tctgaagaag gtaata
//
LOCUS       DAJTXQ010000377          601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000377 DAJTXQ010000000
VERSION     DAJTXQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.383"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            31..>601
                     /locus_tag="OO515_004417"
     CDS             31..>601
                     /locus_tag="OO515_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6578149.1"
                     /translation="MPYQADCVILAGNAVMPTIDAACKIARDQQIPLLISGGIGHSTT
                     FLYSAIAQHPHYNTIRTTGRAEATIQADIAHQFWHIPHEKIWIEDQSTNCGENARFSI
                     ALLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADS
                     VIFINQLQGLWPVERYLSLLTGELPRLRDD"
BASE COUNT          146 a          157 c          152 g          146 t
ORIGIN      
        1 tggctggcgc aggatgattt ctccggtgag gtgccgtatc aggccgattg cgtgatcctt
       61 gcaggcaatg cggttatgcc gactatcgac gcggcatgta agattgctcg cgatcagcaa
      121 attcctttac tgattagtgg tggtatcggt cactcgacaa cttttttgta tagcgccatc
      181 gcacagcatc cgcactacaa cactatccgc accactggca gagcagaagc gaccatccag
      241 gcggatatcg ctcatcagtt ctggcacatt ccgcatgaaa aaatctggat tgaagaccag
      301 tcaacaaact gcggtgaaaa cgcacgcttt agcatcgcgc tattgaatca ggccgtagaa
      361 cgagttcata cggctatcgt tgttcaggac cccaccatgc agcggcgcac gatggcgacg
      421 ttccgccgta tgactgggga caatcccgat gcaccacgct ggttaagtta tcccggattc
      481 gttcctcagt taggaaataa cgcagacagt gtaatcttta ttaatcagtt acaaggatta
      541 tggccggttg agcgttatct ctcactactc actggcgagc tgccgcgttt acgcgatgat
      601 a
//
LOCUS       DAJTXQ010000378          595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000378 DAJTXQ010000000
VERSION     DAJTXQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.384"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>595
                     /locus_tag="OO515_004418"
     CDS             <1..>595
                     /locus_tag="OO515_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578150.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQV"
BASE COUNT          179 a          137 c          146 g          133 t
ORIGIN      
        1 cagtggtaac ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac
       61 attaacgtca ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg
      121 agcgatgaca ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt
      181 agatataacg ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg
      241 gtcatcattg aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat
      301 atcaggcaat ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt
      361 gccctatgtg caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc
      421 cactgttaca ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca
      481 tcaagctggt ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg
      541 tacagtatca gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggtg
//
LOCUS       DAJTXQ010000379          586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000379 DAJTXQ010000000
VERSION     DAJTXQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.385"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..415
                     /locus_tag="OO515_004419"
     CDS             <1..415
                     /locus_tag="OO515_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6578151.1"
                     /translation="SALSPYHENRIALDINDLENDVELKSTSAVAVPRQGSVVFADFE
                     TVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWL
                     EESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
BASE COUNT          195 a          110 c          126 g          155 t
ORIGIN      
        1 cagcgctctt tctccttatc atgaaaaccg tatcgcgctg gatatcaacg atcttgagaa
       61 cgatgttgaa ttaaaaagta ccagtgcagt agctgtaccg cgtcagggtt cagtcgtctt
      121 tgctgatttt gaaaccgtgc aagggcaatc agccattatg aacatcacac gaagtgatgg
      181 taaaaatatt ccatttgctg cagatattta tgatgagcaa ggcaatgtca ttggtaatgt
      241 tggacagggt ggacaagcat ttgttcgtgg cattgagcag cagggaaata tcagcattaa
      301 atggctcgaa gaaagtaaac ccgtaagttg tcttgcgcat tatcaacaaa gttcagaagc
      361 agaaaaaata gcgcaatcta ttattctgaa tggaattcgg tgtcagattc agtaactaca
      421 aggaacgtca aatgataaaa ataacgccac ataaaatagc gatactgatg ggaatattat
      481 tatcaccatc agtatttgca acggatatta atgtagagtt tacagccact gtcaaagtag
      541 actggccccc tgaatctcca gacaaccagt atcacttaaa taagtg
//
LOCUS       DAJTXQ010000380          584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000380 DAJTXQ010000000
VERSION     DAJTXQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.386"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..152)
                     /locus_tag="OO515_004420"
     CDS             complement(<1..152)
                     /locus_tag="OO515_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578152.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDP"
BASE COUNT          176 a          110 c          116 g          182 t
ORIGIN      
        1 ggatcgttac tgtctaccgc tggcattgga ccagctgtca acaaacgacc ttcatcatgc
       61 agtaactgta aacgtgctaa atgtgccgga cgaacggaaa ggcgtttttc gaggctatca
      121 gctttgtctt gagcgtaaat aacatacaac acgggcacaa ctccttgttc gggaaagttg
      181 taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa agatgatgtc tttgttaaga
      241 ccatgcataa agtaaggtca attacgccaa aaatgacatt ttcactgatc ctgatcgtct
      301 tgccttattg aatatgattg ctatttgcat ttaaaatcga gacttggttt ttctactgaa
      361 atgattatga cttcaatgac ccttgaatga agtctattcg gttttctagg agacctgtta
      421 aaaacaggac aatatcaggt gagtccctat gcaatttaat agtagatttc caagtatcta
      481 tgcaattcta cacagattct atacagtctc ggtatagtcc ctttttaccc taacttattg
      541 aaatatataa agaatctgtg aagtctcttt tcagggtaaa ctgt
//
LOCUS       DAJTXQ010000381          580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000381 DAJTXQ010000000
VERSION     DAJTXQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.387"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..195)
                     /locus_tag="OO515_004421"
     CDS             complement(<1..195)
                     /locus_tag="OO515_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6578153.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFL"
     gene            396..>580
                     /locus_tag="OO515_004422"
     CDS             396..>580
                     /locus_tag="OO515_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6578154.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAED"
BASE COUNT          169 a          145 c          138 g          128 t
ORIGIN      
        1 caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca tggtcgccag
       61 ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa atccaccacc
      121 atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa aaataatcca
      181 agcaataatt cccatgttca ggccctcgtt tcgatgaatc ggacgcaaga aatgcttacg
      241 tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtatgtcg gctgatttaa
      301 gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaactg tgaccgataa
      361 accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat gagcagttcc
      421 tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa gccgcaattc
      481 ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca ataaccccgg
      541 ataaactggt gctggatttc ggcagtcaag ccgaagacaa
//
LOCUS       DAJTXQ010000382          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000382 DAJTXQ010000000
VERSION     DAJTXQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.388"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>571)
                     /locus_tag="OO515_004423"
     CDS             complement(<1..>571)
                     /locus_tag="OO515_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6578155.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKL
                     MRIYNQKGIFSNTKDSEEGLTHILSEHFEN"
BASE COUNT          163 a          117 c          122 g          169 t
ORIGIN      
        1 attctcgaaa tgctctgaga gtatatgtgt taagccttct tcggaatctt ttgtgtttga
       61 aaagatgcct ttctgattgt aaatgcgcat cagtttttga ccgaagctat tgtgcacaac
      121 tccatcgcca agaattgtgg ctccgtatag agttccatcg tcagttaagg cctgcgccgc
      181 attgcgtatt acacagcttt ttgtagatat atttccaggc aggcagtgaa gaaggtaaaa
      241 catggaaatg gaatcaaatt gaccatgtaa cgccgcggga taaggttcaa aaacatcatg
      301 gctaatttta tgtttaattt ttgattcccc agcccttgta gatgccgcgt tcaggctagc
      361 ttcgttcaaa tccattaaag atatcagact actctcaggt acgtgagtaa ggtaaaaccc
      421 agttccaaca ccaatatcca gatggttgtt acctaaatgt tccagaaagt gtggaagaag
      481 gtgttccttt gtaggacatc cccatgcaag ccgatttgat actcccaaaa cccaccagtc
      541 ataaagcttt agggtaagtg gtgtgtaaat t
//
LOCUS       DAJTXQ010000383          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000383 DAJTXQ010000000
VERSION     DAJTXQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.389"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..172
                     /locus_tag="OO515_004424"
     CDS             <1..172
                     /locus_tag="OO515_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6578156.1"
                     /translation="VVVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWR
                     PAYAAFVQKLFK"
     gene            187..>565
                     /locus_tag="OO515_004425"
     CDS             187..>565
                     /locus_tag="OO515_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6578157.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          166 a          119 c          142 g          138 t
ORIGIN      
        1 tgtggtcgtt ggtgataaag atgtcgtaac cggcaaggat atcgccgggc tgaaaactga
       61 gcttgagcag aaaaaaatta agtttgatta ccaggaatat cccggcctga accatgaaat
      121 ggatgtctgg cggcctgcct atgcagcctt cgtacagaaa ttatttaaat aaaataaggc
      181 attaagatga aattaatcat taccgaagat taccaggaaa tgagccgtgt cgcggcacac
      241 catctgcttg gttatatgtc gaagacgcgt cgtgttaacc tggcaattac cgccggtagc
      301 acgcccaaag gcatgtatga atacctcacc accctggtta aaggtaagcc ctggtacgat
      361 aactgctatt tctataattt cgatgaaatt ccctttcgcg gcaaagaggg agaaggcgta
      421 acgattacca atctgcgtaa tctgtttttc acccctgcgg ggatcaaaga agagaatatc
      481 cagaagctca ctattgataa ctaccgcgag catgatcaga aactggcgcg tgaaggcggc
      541 ctggatttgg tggtgctggg attag
//
LOCUS       DAJTXQ010000384          558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000384 DAJTXQ010000000
VERSION     DAJTXQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.390"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..518)
                     /locus_tag="OO515_004426"
     CDS             complement(<1..518)
                     /locus_tag="OO515_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6578158.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMT"
BASE COUNT          156 a          153 c           97 g          152 t
ORIGIN      
        1 gtcatccagt actttacttt ttctttatag cgcgtgaaga ccgttgatgc gaagcggata
       61 aaaaagtcga tcagcttacg gtttcgccag ccaccatatt ttgtcaccag atgataaggc
      121 atctcaaaat gcgaaagcgt caccacaggt tccattcctt gcttcaggca ttcatcgaac
      181 agatcatcat aaaattgtaa accctcttca ttcggctcct gttcgtcacc ttgcggaaag
      241 attcgtgtcc aggcaatgga agttcgaaag catttgaatc ccatctcggc aaataactga
      301 atatctgttt tatagcgatg ataaaaatca attgcttcat gattgggata attaagcccg
      361 tcgataacgc cttctgtcac ttcacgcggc accccgtgag cgccagcagt catcacatca
      421 gcgatactga tgccttttcc tccttcattc cagccacctt ccaactgatg cgcggctacc
      481 gcgccacccc ataaaaaacc ttttttaaat cctgacatac acaactcctt aactaaaata
      541 gattaccacc agacttcc
//
LOCUS       DAJTXQ010000385          556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000385 DAJTXQ010000000
VERSION     DAJTXQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.391"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>556
                     /locus_tag="OO515_004427"
     CDS             <1..>556
                     /locus_tag="OO515_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6578159.1"
                     /translation="PGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQAAAVHTLY
                     GYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTPVLPAGTQI
                     QRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSGLPSTGYAD
                     TITDGEDIENLEIWRARVMERYYYI"
BASE COUNT          125 a          138 c          170 g          123 t
ORIGIN      
        1 cccggcctcc cgcaacttat ttctatgatc cggagcgatt tattaacccg ctttgagcag
       61 gatacgctgc ttcgtcgtat ggatgcggaa gtgtatgccc gtgtacaggc tgcagccgta
      121 cacacgttgt acgggtatat cgattatctt gccagaaatc tgttaccgga cgtgtgtgat
      181 gaagactggc tgtaccggca cgccagaatc aaacgctgcc cgcgaaaaga agcggtggca
      241 gcccggggat ttgtgcgctg ggatggcgta gaggggacgc cggtattgcc agcgggaacg
      301 cagatccagc gtgataatca ggtgaccttt accacgacgg cgacggtgac cgcagccgat
      361 ggtcttctcc gggtgcctgt tgtggcagac gaaccgggat cggcggggaa tacggatgat
      421 ggtattgcca tgcaactggg aacacccgtc agtggtctgc cgtccacagg gtacgctgac
      481 accattacag acggtgaaga tattgaaaat ctggaaatat ggcgtgcccg cgttatggaa
      541 cgttattact acattc
//
LOCUS       DAJTXQ010000386          547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000386 DAJTXQ010000000
VERSION     DAJTXQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.392"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..489)
                     /locus_tag="OO515_004428"
     CDS             complement(<1..489)
                     /locus_tag="OO515_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6578160.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLG"
BASE COUNT          152 a          156 c          139 g          100 t
ORIGIN      
        1 cccaagtttg tgagtgatga tcgggccaag caccatcgca atggcaaagg taatgccaaa
       61 gctcacgccg ataaacgcca tcgctttggt gcggttttgt tcgcgcgtga gatcggaaag
      121 cagcgccata acggcggcgg caattgctcc agagccttgt agcgcccggc ccagaataat
      181 tccccagatg gagtcggaaa gcgcagcgat aacgctaccg gcggcaaaca ccgccagccc
      241 accgacaatt aatggtttgc gaccaatgcg gtcggaaagc aggccaaacg gaatctgaaa
      301 aacggcctga gtcagaccat aaataccaat ggcaataccg attaatgctt cgctggcacc
      361 ttgcagagcc atgccgtacg tggtcagaac cggcagaacc ataaacatgc ccagcatgcg
      421 caacgagaat acggtcccta aaccccaggt cgcgcgcctc tcacctggcg tcattttata
      481 atcgttcatt accacccctt aaaaaaatgc ccacatagtg tagtgcggag cgcatgttgg
      541 gtaaaca
//
LOCUS       DAJTXQ010000387          547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000387 DAJTXQ010000000
VERSION     DAJTXQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.393"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..547
                     /locus_tag="OO515_004429"
                     /pseudo
     CDS             1..547
                     /locus_tag="OO515_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          194 a          120 c           83 g          150 t
ORIGIN      
        1 tccgacttat taaaaatgcc tgtttaaaat tatataattt agcacatacc gcaaccaact
       61 cccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa aagctattct
      121 ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct gaaaaacagt
      181 cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatggt acaaacttct
      241 gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag aagatcgccg
      301 ctaatgcaaa aaatactcaa gtcaattatc ctactggtct ctacgtcccc tactccacca
      361 gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca tggccgaatg
      421 aagtccagtt attccccata aatactggcg gacactggat attagtttcg ctacagaaaa
      481 tagtaaataa aaaaataata aactacaaat aaaatgcgtc atattcaact cattgcgtgc
      541 actaggc
//
LOCUS       DAJTXQ010000388          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000388 DAJTXQ010000000
VERSION     DAJTXQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.394"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..544
                     /locus_tag="OO515_004430"
                     /pseudo
     CDS             1..544
                     /locus_tag="OO515_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    121..237
                     /locus_tag="OO515_004430"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          130 a          132 c          173 g          109 t
ORIGIN      
        1 tgagcgggat accaggggcg gtgatggagg gctcaccacc gctgaacgtc agcgtctgaa
       61 agagctggag cgtgagaatc gtgaactgcg ctgcagtaac gatatccttc gccaggcttc
      121 cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaataatgcc actgctggat
      181 aagctgcgtg ggcagtacgg ggtcggaccg gtatgcagtg aactgcatat tgccccgtca
      241 acgtattacc actgtcagca acaggacatc atcctgataa acgcagtgcc cgtgcgcagc
      301 acgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat caggtgtacg
      361 gtgtgcgtaa agtctggcgc cagttgttac gggaaggaat cagggtggcc agatgtacag
      421 tggcgcgcct catggcggtt atgggacttg tcggtgttct ccggggtaaa aaggtccgca
      481 ctaccgtcag ccggaaaacc gttgccgcag gtgaccgcgt aaaccgtcag ttcgtggcag
      541 aacg
//
LOCUS       DAJTXQ010000389          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000389 DAJTXQ010000000
VERSION     DAJTXQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.395"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..>538)
                     /gene="fimZ"
                     /locus_tag="OO515_004431"
     CDS             complement(26..>538)
                     /gene="fimZ"
                     /locus_tag="OO515_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR6578161.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          169 a           93 c           96 g          180 t
ORIGIN      
        1 tatttatgat caattataat taatattata ttaattcgta taatttggcg tagtcgataa
       61 gctctacaat tgaatgcaaa cctagtttgc cataaatatt agatttatgc gcactaactg
      121 ttttattgct aagtaataac ttatcggcaa tttctttatt agataatccg ctaaccagat
      181 aacgtaatat ggtcacttca cgattagata gcacagtgac cgttgaacta ttcgtactac
      241 atttattgct ttttatatag ttgagcgttt cgctaggaaa aaacgtgtat ccggagagga
      301 tcatctgaac gggatgaaaa atatcattct gatcattgca tttactgaca aaaccgttag
      361 caccagcttg tatcgctctg ccagcataaa agcattctga tttcgatgat aaaaataaca
      421 ctttcactgt gctctggatt tgtttgatcc ttttcaggaa ggtaaaaccg tctgttccgg
      481 gcaagtctat atccataatg attaaatcaa caggacgggt tcggagataa tcgatggt
//
LOCUS       DAJTXQ010000390          537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000390 DAJTXQ010000000
VERSION     DAJTXQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.396"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>537)
                     /locus_tag="OO515_004432"
     CDS             complement(<1..>537)
                     /locus_tag="OO515_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCR6578162.1"
                     /translation="STLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNAMFRN
                     NIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPPG
                     YTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYG
                     DGPSTAAGGFMYLGLSEVT"
BASE COUNT          149 a          128 c          112 g          148 t
ORIGIN      
        1 gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca
       61 tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct gtagctgtca
      121 tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat cgccccgcca
      181 taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata acctggagga
      241 tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa
      301 cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata gacgtcatta
      361 ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg
      421 ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata gattgcgcca
      481 ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag cgttgag
//
LOCUS       DAJTXQ010000391          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000391 DAJTXQ010000000
VERSION     DAJTXQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.397"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>532
                     /locus_tag="OO515_004433"
     CDS             <1..>532
                     /locus_tag="OO515_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6578163.1"
                     /translation="RRKYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRK
                     TMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDT
                     QGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIR
                     LTSENGSHTTFRYDVLD"
BASE COUNT          131 a          155 c          153 g           93 t
ORIGIN      
        1 cgccgcaaat atgatgaatc cggccgtctg attcaggaaa ccgcccctga cggcgatatc
       61 acccgctacc gttatgataa tccacacagt gacttaccct gcgcaacgga agatgccacc
      121 ggcagccgga aaaccatgac atggagccgt tacggtcagt tgctgaccgt cactgactgt
      181 tccggttatg taacccgtta tgaccatgac cgttttggtc aggtgacggc ggtgcaccgc
      241 gaggaagggc tgagccagta ccgcgcatac gacagccgtg gacagttaat tgcggtgaaa
      301 gacacgcagg gccatgaaat gcggtatgaa tacaacgccg ccggtgacct gaccgccgtc
      361 attgccccgg acggcagcag aaacgggaca cagtacgatg cgtggggaaa agccatctgt
      421 accacgcagg gcgggctgac gcgcagtatg gaatacgatg ctgccggacg ggtcatccgt
      481 ctgaccagtg aaaacggcag ccacaccacc ttccgttacg atgtactcga cc
//
LOCUS       DAJTXQ010000392          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000392 DAJTXQ010000000
VERSION     DAJTXQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.398"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            86..334
                     /locus_tag="OO515_004434"
     CDS             86..334
                     /locus_tag="OO515_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578164.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(331..>532)
                     /locus_tag="OO515_004435"
     CDS             complement(331..>532)
                     /locus_tag="OO515_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578165.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          118 c          125 g          153 t
ORIGIN      
        1 gacttctggt tcgctcaatg gttcttacac aaacttaggt tcacatagag gttcattctc
       61 agggcggaat tcagggggca gtacattgtt tatttatgca tctggaggta atggaggatc
      121 tgccggaggt gcatgtgcaa atacatcccg actgcaggga tatgttggtg ggacgctaat
      181 tagcgtgaat gccagcaata accctgcata tggaaaaaca gcctttatca gctttgctgt
      241 acctgcaggt acttcctatc agataacatc ctatccaaca gaaaatacat catgtggtgc
      301 cggggtattt tcagtatttg gatatcaaac ttaattgagc gttacacacg acgcaataca
      361 acctgtctta tgctcgtatt tgtaccattt acgtgaacct gctgggctct caactacctg
      421 gcagtagtgg gcatcctctg cgttgcccat tctggcaatg gcacagaagt cgaattgccc
      481 cagataacct gtggtggaaa gctgagtaac tttgagtttt ccacctattg tc
//
LOCUS       DAJTXQ010000393          531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000393 DAJTXQ010000000
VERSION     DAJTXQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.399"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(72..148)
                     /locus_tag="OO515_004436"
     tRNA            complement(72..148)
                     /locus_tag="OO515_004436"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(162..238)
                     /locus_tag="OO515_004437"
     tRNA            complement(162..238)
                     /locus_tag="OO515_004437"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(202..204),aa:Arg,seq:tcg)
     gene            complement(246..321)
                     /locus_tag="OO515_004438"
     tRNA            complement(246..321)
                     /locus_tag="OO515_004438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Ile,seq:cat)
     gene            complement(424..>531)
                     /locus_tag="OO515_004439"
     CDS             complement(424..>531)
                     /locus_tag="OO515_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6578166.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          148 a          134 c          129 g          120 t
ORIGIN      
        1 aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa gcgagccagg
       61 gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct tagaaggcaa
      121 ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc atcggggact
      181 tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct aatggcggta
      241 tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc
      301 tcaccactga gctaaagggc cgggagcaga ataataacgg tccgtaatta attccgcaat
      361 aaaaaacccg ctcggcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca
      421 tagttatgac gaatttacca cagattccgg aaaaatcaac ctagttacct tttttaactg
      481 ccgctcagcc catgcttctt caatatcaaa ccgagtcacc agcgcatcat a
//
LOCUS       DAJTXQ010000394          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000394 DAJTXQ010000000
VERSION     DAJTXQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.400"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..174)
                     /locus_tag="OO515_004440"
     CDS             complement(<1..174)
                     /locus_tag="OO515_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6578167.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVRVRQHERDT"
     gene            272..>526
                     /locus_tag="OO515_004441"
     CDS             272..>526
                     /locus_tag="OO515_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578168.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDW"
BASE COUNT          136 a          134 c          133 g          123 t
ORIGIN      
        1 ggtatcccgc tcatgctggc gaacccggac acgcagagtc tccggcgtac agccaatctt
       61 tggagcaatg gaacaaattg tcgcccattg tgagtcatat tcgccctgac tttccagaac
      121 catacggact gcccgttgac ggacttcggg ggaaaaacga gtatttttag tcatcctgtt
      181 tacctctttc tcaggaagtt tagtctccag gattcccggg gcggttcaat gatttttacc
      241 tgtcagaaca ttatcagatg gaacgtgcag gatggatcag gaaatgacgt tttcactcag
      301 ctatgaacaa ctgacccggt ttgcggaaaa acgcatccgg gaatgcaatc tggacagcca
      361 cggcgtaact tatctctgcg agtcagccaa agccggagcc gtgctgatct tctggcatga
      421 actggccata aatggctata cctcaatgaa tgccataaag agacaggaga tcattgacgc
      481 cgaccatcag cgcctcagaa agctcatctg gccggaggat gactgg
//
LOCUS       DAJTXQ010000395          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000395 DAJTXQ010000000
VERSION     DAJTXQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.401"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>520
                     /locus_tag="OO515_004442"
     CDS             <1..>520
                     /locus_tag="OO515_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6578169.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJTXQ010000396          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000396 DAJTXQ010000000
VERSION     DAJTXQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.402"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..377)
                     /gene="mdtL"
                     /locus_tag="OO515_004443"
     CDS             complement(<1..377)
                     /gene="mdtL"
                     /locus_tag="OO515_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6578170.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRA"
BASE COUNT          154 a          134 c          139 g           92 t
ORIGIN      
        1 gcccgacgtc gatcatccag cgtgtcgcgc aaaatggcga acgccactac gtaacaacag
       61 cctgcgccca acccctgtag aaatcggcct gcaagaaata acgtgctggt ttcagccagt
      121 gaacagaaca ccgaggcaat aataaatagc gccgcgccgg gtatggcgac cggctttctc
      181 cctgaacgat cggccacttt accggcaaat aacatcgcag ctgccatccc cgccagatat
      241 acggagaacg caatatgcaa ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt
      301 aaaccaacga ggtacatatc aatcccggcg ggataaagta aaaccagggc aaaactacaa
      361 atcaaaaagc gggacatggg ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg
      421 gtgaaggagg atcaggcgag ttgccatttg gacataagtg atttcctgag aggcaagaag
      481 ccagcgaatg gctggcttct tgaaggattt agccaaatt
//
LOCUS       DAJTXQ010000397          507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000397 DAJTXQ010000000
VERSION     DAJTXQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.403"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          134 a          110 c          127 g          136 t
ORIGIN      
        1 cgttttatca aaaatataag agacaaattc ttctttagtc atatagattt ccttcttggt
       61 tattcgggat aagaaggata ccacctcgcc tgacgtggtt aaaagcaggc acacaacgcg
      121 aaatgacgcg gcggtaagta tggcggagtt attccttccc cgttgaggac accgggttgt
      181 caggttgacc atacgcttaa gtgacaacca cgccacaaca ctccatgttg attggtacct
      241 ttggcggcat cagtttcatt gctggctgat gtccgccctt tttaaagtga attttgtgat
      301 gcggtgaatg cggctcagcg cacgcggcac agttaaaaag cgattagttc ccccgtatcg
      361 ggtggttatg ggtttccctg tatccggcgt taattgttaa ctggttaacg tcacctggag
      421 gcaccaggca ccgcatcaac aaagttcact tcggtgatga aaggaaagag aaaatgttga
      481 atgtagctat tgaaaaccag aacgggt
//
LOCUS       DAJTXQ010000398          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000398 DAJTXQ010000000
VERSION     DAJTXQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.404"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..188)
                     /locus_tag="OO515_004444"
     CDS             complement(<1..188)
                     /locus_tag="OO515_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6578171.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="OO515_004445"
                     /pseudo
     CDS             242..445
                     /locus_tag="OO515_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          134 c          117 g          129 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggatttc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaa
//
LOCUS       DAJTXQ010000399          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000399 DAJTXQ010000000
VERSION     DAJTXQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.405"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..485)
                     /locus_tag="OO515_004446"
                     /pseudo
     CDS             complement(1..485)
                     /locus_tag="OO515_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          170 a          114 c          106 g          113 t
ORIGIN      
        1 ttagcaatgc tagcataaaa tggcagcaat ggtttgatta aatgttgaat cttcacaccc
       61 gagatagaac aaccaacaaa tagcgcacta cccaccggcg gtgttaacag cccaatacaa
      121 aggttggcca ccatcataat accgaaatgt actgggtcca ttcccagctc ttgtgcaatc
      181 ggcaagaaga tcggtgtaaa gattagtaca gccggtgtca tgtccataaa gatgccgaca
      241 attaataaga cgatattaat cagcagcaat atgatcagtg ggttatcaga aatccccatc
      301 aatgcgtcgc tgatcatata aggaatatca gcattagtca ttgcccagga catgcccacg
      361 gaaaaaccga tcaacagtaa tacaatcgaa gtggtaacaa ccgactccag aatcagtttg
      421 ggtaaatggc gccattttac ttcccggtaa atcagcaccg agagaataaa tgtgtagaca
      481 acggcggtga tgctgccaac tta
//
LOCUS       DAJTXQ010000400          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000400 DAJTXQ010000000
VERSION     DAJTXQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.406"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          137 a          104 c           68 g          185 t
ORIGIN      
        1 atgacctacc cctagttcac tgtgagctac cgttacatcc aatcatcttc cgatgtaacg
       61 tatcgttact accgatgtgt aatatcgtta cattcaataa ttagtctatg taatatatcg
      121 ttactaccaa ctttcttaca gagagcattt cagataaatg aatgtaacct ctcgttacat
      181 ccatttgtca catagcactc tgtgtaatgt ctttctatgt gtgtgccata atccccctat
      241 attccctgtg taaatcatag aataatgctc tctattccct gtgtaattcc ctactaaaaa
      301 atccaatgaa aagtagctta aaagttcaat tcttactttc ttcactttta catcactttc
      361 gcaaagcctt gtgtaatgcg gcttacagag caccatcaga aattgttggc taaaaattgt
      421 tctgtttgtt tctgaacgag tttctcgttt gtgcaaattt taaaatttat tttgttttta
      481 tagccttatt tcgt
//
LOCUS       DAJTXQ010000401          492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000401 DAJTXQ010000000
VERSION     DAJTXQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.407"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..>492
                     /locus_tag="OO515_004447"
     CDS             30..>492
                     /locus_tag="OO515_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6578172.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRL"
BASE COUNT          166 a          115 c          102 g          109 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact ta
//
LOCUS       DAJTXQ010000402          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000402 DAJTXQ010000000
VERSION     DAJTXQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.408"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="OO515_004448"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="OO515_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6578173.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAJTXQ010000403          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000403 DAJTXQ010000000
VERSION     DAJTXQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.409"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          137 a          109 c          105 g          132 t
ORIGIN      
        1 atcatcatca gcgtcgatat ggacgaatat aaaaacattt acgttatatc ccaaaaacac
       61 acaggtctta gggaggttta aactgtgtat taacacctat cgtattgacg ggcgtgaaat
      121 ggcagaaaca gaagtcgttc ctattgacat gcctgactct aacggcgaga tgacttggca
      181 agccaaaaac tacacacagt attcgtccta ctttatgaag attacttgct tgaaatagct
      241 tattggcaaa tggcatatac agttattgaa ccattctgat cttcggttgt atcgcacgca
      301 gccgaccatg agttattggc tgatgggaat gacctggtaa gccatatgta accagttcgt
      361 gaggtgcata ttccgccgcc acctgtaaca cgttttccag ttggacaggt agctgttgct
      421 ccaggaaagc gccaacctgt cgcctgtgct gtagcaattt gaagctcacc agatcctacc
      481 ttt
//
LOCUS       DAJTXQ010000404          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000404 DAJTXQ010000000
VERSION     DAJTXQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.410"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..308)
                     /locus_tag="OO515_004449"
     CDS             complement(<1..308)
                     /locus_tag="OO515_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6578174.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     P"
BASE COUNT          141 a          114 c          102 g          121 t
ORIGIN      
        1 ggctgaaact cgtaaccacg ggcgaaatca tgcaggcagg cgtaaacctc ttcgaaatcc
       61 cacgggttat gcagttcaat ctcaacgccc acgacttctg tttctggcga aacgctggcg
      121 atatcgcgtt taagcgtttc gaatagcgag cgcgagcgag tgtcgtgcaa caattccagc
      181 cgatcgatga ctaacgattc ttgctggcat aaacaaagcg tcggacgcca tttcgaccag
      241 cgctgactgc cgcgtccggc ataatccagc acggtaccga caaagccaaa agccactgtt
      301 ttacgcatct tagatatcct tataaaagac gatatttcat tatcggtgaa ttggttttgt
      361 ggaaaatctc cgtttacgga cgtaaagatt taacctattt aaaaacaatg aattaaaaaa
      421 ttaatctgct tatttaattt tctggcacga cggttgcaat tatcacgaca gcaaacaa
//
LOCUS       DAJTXQ010000405          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000405 DAJTXQ010000000
VERSION     DAJTXQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.411"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>477
                     /locus_tag="OO515_004450"
     CDS             <1..>477
                     /locus_tag="OO515_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578175.1"
                     /translation="IKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAVIMYCLSLR
                     RMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMFLAQRERRL
                     YQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTKILEIDSR"
BASE COUNT          121 a          116 c          133 g          107 t
ORIGIN      
        1 atcaaacgat taccgttggt tggcgtctgg ctggaacggc gactggcggc caaatcctcg
       61 aaaacccaaa gcatgaaaac caaatctccg gcgctatggg cggcggtgat tatgtactgc
      121 ttatcgctgc gaagaatggc gaatctacgc aaggttcagc gactggcgca aagtggcgtt
      181 ctggtggtca gcgatcgctt cccgcaggct gaaatttcgg gcttttatta cgacggaccg
      241 gggattggcg tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg
      301 cgtttatacc aacaaatggc gcaatatcgc ccggaattaa ttattcgcct gggcattgat
      361 attgagactg ccatctcccg caagcctgac catgactatg ccgagctgca ggacaaaatc
      421 ggtgtcatgt cgaagattgg ctataacggc acaaaaattc ttgagattga ttccaga
//
LOCUS       DAJTXQ010000406          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000406 DAJTXQ010000000
VERSION     DAJTXQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.412"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>476
                     /locus_tag="OO515_004451"
     rRNA            <1..>476
                     /locus_tag="OO515_004451"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJTXQ010000407          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000407 DAJTXQ010000000
VERSION     DAJTXQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.413"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>473
                     /locus_tag="OO515_004452"
     CDS             <1..>473
                     /locus_tag="OO515_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6578176.1"
                     /translation="DGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGET
                     AERWRYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETR
                     HAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT          109 a          146 c          155 g           63 t
ORIGIN      
        1 tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga
       61 tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt
      121 gaagggtgaa accgcagagc ggtggcggta cgacgaacgc ggctggctga cagacatcag
      181 ccatctcagc gaagggcacc gggtgacggt gcattacggg tatgatgaga aaggccggct
      241 gaccggtgag cgtcagacgg tgcatcaccc gcagacggaa gcactgctct ggcagcatga
      301 gaccagacat gcgtacaacg cgcaggggct ggcgaaccgc tgtataccgg acagcctgtc
      361 cgccgtggaa tggctgacct acggcagcgg ctggctgtca ggcatgaaac tcggcgacac
      421 accgctggtg gagtacaccc gcgaccgcct gcaccgggaa acgctgcgca gct
//
LOCUS       DAJTXQ010000408          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000408 DAJTXQ010000000
VERSION     DAJTXQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.414"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..157)
                     /locus_tag="OO515_004453"
     CDS             complement(<1..157)
                     /locus_tag="OO515_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578177.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATA"
     gene            complement(280..>470)
                     /locus_tag="OO515_004454"
     CDS             complement(280..>470)
                     /locus_tag="OO515_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6578178.1"
                     /translation="GPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDK
                     GNDFFQQIVNKQCANCTK"
BASE COUNT          115 a          115 c          132 g          108 t
ORIGIN      
        1 tagcggtggc aagcagtagc ggcacgacaa taatcaccgg tgtcacttcc atcagccagg
       61 tgagtttgtc atgggccgaa atgccggtat aaatcaggat taacgttagc gccagtgcgc
      121 cggtgttaag aattaacggt ttgagtgtgc gggtcatggt gagttcgcaa gtcagggaaa
      181 atactgacta ttcaccagca agcatgaaat tacaatcgca aaagaggtgg cccaggggga
      241 tcacctggca gcatgctgcc aggcgctggg ccgaagaggt tacttagtgc agttcgcgca
      301 ctgtttgttg acgatttgct ggaagaagtc gttacctttg tcatcgacca ggataaacgc
      361 cgggaaatct tctacttcga ttttccagat agcttccata cccagctccg gataagcgac
      421 gcattccaga tgcttgatgc tctgctgcgc cagtaccgcc gccggaccgc
//
LOCUS       DAJTXQ010000409          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000409 DAJTXQ010000000
VERSION     DAJTXQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.415"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(225..452)
                     /locus_tag="OO515_004455"
     CDS             complement(225..452)
                     /locus_tag="OO515_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578179.1"
                     /translation="MGLFFLRSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELI
                     LYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          153 a           99 c           86 g          131 t
ORIGIN      
        1 agagagccag cggcaggctc aggacgataa tccggggaaa atataccact cgcagagtgt
       61 taacatttga catcggattg tctatcctgc attggtgtcg agttggcaag gctgaggata
      121 atgctgagga taatgctgag gataatgcag gatagacaga gcactagaga ttgcatgcaa
      181 tctcgtacct atcacctcag cctgccgctg gctcttttcc agaactaaga atgcgttctt
      241 aaaaatccaa acttgatacc gtttaaatct attttcacaa aaagagtttt taccctctcg
      301 tttgttttct gaatatataa aattaattca tcctcaaaac cttcgtgcgg atgaaaacca
      361 aatgtgccaa aacttaatcc atcaataagc aaaataaggt ttttaaacct gtcaggaatc
      421 accgcttcac ttcttaaaaa aaataaacca atagtcttat ttccattca
//
LOCUS       DAJTXQ010000410          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000410 DAJTXQ010000000
VERSION     DAJTXQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.416"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>469
                     /locus_tag="OO515_004456"
     CDS             <1..>469
                     /locus_tag="OO515_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6578180.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTF"
BASE COUNT          128 a          102 c          116 g          123 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttc
//
LOCUS       DAJTXQ010000411          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000411 DAJTXQ010000000
VERSION     DAJTXQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.417"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..>464
                     /locus_tag="OO515_004457"
     CDS             231..>464
                     /locus_tag="OO515_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578181.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSA"
BASE COUNT          140 a           72 c          114 g          138 t
ORIGIN      
        1 agcatcttta ttatgattcg ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt
       61 tctaataaaa tagattgggt gggttgtata ttgacgttag tataaatagt attaatgccg
      121 cttgcatgaa tcaagcaaaa tttatttata ttagtaatat gtatttaatt aataaataca
      181 tacattattt ttatgattaa ggataatcaa taattaaaac aggaagttct atgtctacga
      241 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
      301 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      361 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      421 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcc
//
LOCUS       DAJTXQ010000412          462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000412 DAJTXQ010000000
VERSION     DAJTXQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.418"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            89..451
                     /locus_tag="OO515_004458"
     CDS             89..451
                     /locus_tag="OO515_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6578182.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          111 a          110 c          136 g          105 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg ag
//
LOCUS       DAJTXQ010000413          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000413 DAJTXQ010000000
VERSION     DAJTXQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.419"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..445)
                     /locus_tag="OO515_004459"
                     /pseudo
     CDS             complement(1..445)
                     /locus_tag="OO515_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          125 a          101 c           99 g          132 t
ORIGIN      
        1 caaccactaa tgcaacagga accacttcat agaattgcag cccttctttg ctggtatcta
       61 caccggtaat ggccccacgg aaaatcagac tacgcggccc agcggtggta accagtggtt
      121 tacgctgcgc tatcgcttct ttcatttcgg tgttggtata gttcaaaatt ttatccagaa
      181 ctttctgccc tacctaggta gaaggttttg gcaccggata ataagtgatt gggttccaga
      241 cgatgctgtc atatttgctt tgatcaaaac tcgggtctac ccaacgtaaa acaggtttac
      301 ctgttgctga ggttgtttct tttaaatcag agtaattgtt taaaaaacca gaatatttat
      361 ctggctgggt gattttagag gcacaaccag acagagccaa caagccgcat aaagctgcaa
      421 ctttcgcaaa tgatgtggta cgcatgaata gtttcct
//
LOCUS       DAJTXQ010000414          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000414 DAJTXQ010000000
VERSION     DAJTXQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.420"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          129 a          105 c           87 g          130 t
ORIGIN      
        1 cgctcctaaa attcaattca caacgcagac ctacaacata gctaaaaaca ctcgtaattt
       61 aaggctcggg gtccatgcat attgctcatg gacttacctg aatggctcac cctttggtgg
      121 ttttcaacag gtatattccg accaaaacaa cgtttggtat gtgagtaatt atgcttgggg
      181 aaattatgag tctggtggga ccatatcagt cacatgcctc aatcttcctg gtgctggagt
      241 ttaattaaag gggcaacagt aggcatcgac atgttcctca tcaacaccat caccaatacc
      301 gtacatgcgt gttccagtca taatgtatcc agtcggacat tgaatcggct tgtaatagat
      361 cgaaccacca ctcttaccgc cgataaagtg attcattgct acggatgatt tgtaccaatt
      421 acatgctgag taattaattt tattaccccc t
//
LOCUS       DAJTXQ010000415          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000415 DAJTXQ010000000
VERSION     DAJTXQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.421"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>450
                     /gene="gspA"
                     /locus_tag="OO515_004460"
     CDS             <1..>450
                     /gene="gspA"
                     /locus_tag="OO515_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCR6578183.1"
                     /translation="LTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQW
                     RMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPVVKQD
                     MTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          107 a          103 c          129 g          111 t
ORIGIN      
        1 tttaacctcc agagttctga aacggatagc tttattatgt cggggcgatc gcagaaagct
       61 ggcgcttgcc ggcgaaacaa ttcgcttatt acagcaagcc gagcagacca gcgttttcac
      121 tgcaaaacaa tggcgaatga tttaccgcat attgggtgat aaccgccccc ggaaaatgca
      181 gcttgccgtg gtaatgagtg gaaccattat cgctattacc tgtggttggc tactgctgtc
      241 ctctttcaca gctccgttcc ccgttcctgc gtggctggta cccgtaacgc cagtagttaa
      301 acaagatatg actaaggata ttgctcatgt ggtgatgcgc gatagtgagg cgctaagcgt
      361 gttatacggc gtatgggggt acgaggtccc agctgattcg gcctggtgcg atcaggcagt
      421 aagagcgggg ctggcatgta aaagtgggaa
//
LOCUS       DAJTXQ010000416          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000416 DAJTXQ010000000
VERSION     DAJTXQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.422"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(142..354)
                     /locus_tag="OO515_004461"
     CDS             complement(142..354)
                     /locus_tag="OO515_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578184.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          121 a           94 c           87 g          138 t
ORIGIN      
        1 cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg atcagttgta
       61 tatgcgccat cgtagttctc cgctggcgca gtagaatggg tgttcagccc gttatgtagt
      121 ataccagaat taatgccaat actaacagga tgctctgact cgcaattcat ccagcagttt
      181 atcatttccc ataatgtcac ttaccctcat cggtaaaaaa attgcctttc gaccattacg
      241 atacatcatt gattttttgg tttcagggaa gtaatccatt tcgactataa ctgacaggtc
      301 atcacgacgt atgactgcgt atttactact aaatagtttc tttatttttt ccacgatgcc
      361 tccaggttta taagtacaaa cggttatatc aacatagaga caaaaggtga tgctgccaac
      421 ttactgattt agtgtatgat
//
LOCUS       DAJTXQ010000417          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000417 DAJTXQ010000000
VERSION     DAJTXQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.423"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>439
                     /locus_tag="OO515_004462"
     CDS             <1..>439
                     /locus_tag="OO515_004462"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6578185.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEH"
BASE COUNT          124 a          108 c          108 g           99 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacat
//
LOCUS       DAJTXQ010000418          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000418 DAJTXQ010000000
VERSION     DAJTXQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.424"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..>435
                     /locus_tag="OO515_004463"
     CDS             7..>435
                     /locus_tag="OO515_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6578186.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLG"
BASE COUNT          104 a          100 c          124 g          107 t
ORIGIN      
        1 taaactatga ataacattgc gccgcaatca cctgtaatgc gtcgcctgac gctgctggat
       61 aatcctgcta tcgcccgcgt cattcgtcag gtatccgccg aatacggtct taccgctgat
      121 aaaggctaca ccgtcgccga tccgaatctt gacgagctgt atcaagtgta tagtcagcct
      181 ggccatgcat attgggtggt tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg
      241 ccattaaccg ggagtgagtc ggacatttgc gaactgcaaa agatgtattt tcttccggct
      301 attcgcggta aagggctagc gaaaaaactg gccttaatgg cgatggagca ggcacgagag
      361 atgggcttca aacgctgcta tctggaaacg accgcttttt taaaggaagc cattgcgctt
      421 tatgagcatt tgggc
//
LOCUS       DAJTXQ010000419          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000419 DAJTXQ010000000
VERSION     DAJTXQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.425"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(205..>434)
                     /locus_tag="OO515_004464"
     CDS             complement(205..>434)
                     /locus_tag="OO515_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HCR6578187.1"
                     /translation="IDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIPAALDNVKTS
                     LPTDFPENVVTAVETNVLRLHGRLSREYGSK"
BASE COUNT          132 a          114 c           75 g          113 t
ORIGIN      
        1 gaatggcagt atattcgtgc cacgataaca tctttaaatc acaaggacac ctaatctgaa
       61 cgaataacga ctaccagaaa agcacttcgc atcactcaga catgatttaa cataatatac
      121 attatgcgca caaacataaa ccaagggaga atccagtcgt tggccggtca tgattgctca
      181 ttaacaacga ccaaacccca tatctcactt actaccgtat tctcggctta accgtccatg
      241 caacctcaac acattggttt caactgccgt caccacgttc tccggaaaat ctgtcggtaa
      301 tgaagtcttc acgttatcca gtgctgccgg aatcattctg gcaaagtcac ttaggatttc
      361 atgcatctgc acttccggga atctcagcac ctttgctgtc gccaaaaaat gtcgcggata
      421 aattttatcg attg
//
LOCUS       DAJTXQ010000420          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000420 DAJTXQ010000000
VERSION     DAJTXQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.426"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            38..250
                     /locus_tag="OO515_004465"
     CDS             38..250
                     /locus_tag="OO515_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578188.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..>433
                     /locus_tag="OO515_004466"
     CDS             283..>433
                     /locus_tag="OO515_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6578189.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTEL"
BASE COUNT          151 a           69 c           95 g          118 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgt
//
LOCUS       DAJTXQ010000421          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000421 DAJTXQ010000000
VERSION     DAJTXQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.427"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            335..>422
                     /locus_tag="OO515_004467"
     CDS             335..>422
                     /locus_tag="OO515_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6578190.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCM"
BASE COUNT          133 a           64 c           92 g          133 t
ORIGIN      
        1 cccatttcat gctggtaagt gaaatgccgc acggtgggca gaaactttct ggttacggca
       61 aggatatgtc actttatggg ctggaggatt acaccgtcgt ccgccacgtc atggttaaac
      121 attaagttag atgttagtgg ctgttatagg acggccacta tcgttttagg ttctgagttt
      181 aatggcggtt aagtattaga atgattacat taataactag cgtaaaaatt aggctaaatg
      241 tttgatggcg atttttcatc ctgtcacagt tgattagcat aaggctttag agtatcgtta
      301 ttaaactaat aatttataag tagaagtatt aacaatgaaa gttcaattgt acaaattcac
      361 ggaagataaa aataaaaccc ttaccttcag atggacgaaa aaacactttg agttttgtat
      421 gg
//
LOCUS       DAJTXQ010000422          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000422 DAJTXQ010000000
VERSION     DAJTXQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.428"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            130..>410
                     /locus_tag="OO515_004468"
     CDS             130..>410
                     /locus_tag="OO515_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6578191.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          132 a           83 c           92 g          103 t
ORIGIN      
        1 aagaaatctt gaaagcatat ctggctacga caaaatcgtt gtcaaatata ataactggat
       61 caccaaagat aatggcgagt ttgaactggc ggtccattaa ttatcattgt aataaacgaa
      121 ggaatagtaa tgaaaaaggt atttgcaaaa tctcttctgg tcgcagcgat gttttctgtt
      181 gcaggctccg cattggcagt acaaaaggat attactgtaa cggccaacgt tgatgccgct
      241 ctggatatga cacagaccga taacaccgcg ttgccgaaag cagtagaaat gcaatatctg
      301 ccggggcagg gtctgcaatc ttaccagttg atgaccaaaa tctggtctaa cgacgttacc
      361 aaagatgtaa aaatgcagtt agtttccccg gcgcaactgg ttcaaagcct
//
LOCUS       DAJTXQ010000423          407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000423 DAJTXQ010000000
VERSION     DAJTXQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.429"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>407)
                     /locus_tag="OO515_004469"
     CDS             complement(<1..>407)
                     /locus_tag="OO515_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6578192.1"
                     /translation="AIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDKLGHFFQGLVP
                     ALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQGADDFLGTQG
                     DQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
BASE COUNT           93 a          137 c          102 g           75 t
ORIGIN      
        1 gcgccgtagc tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag
       61 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
      121 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
      181 tatcgccagc gccacgcagc agactaggaa cgccaccatt ttacgtccgc gcacgtacat
      241 cccgcgcacg agaatttctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
      301 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
      361 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgt
//
LOCUS       DAJTXQ010000424          407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000424 DAJTXQ010000000
VERSION     DAJTXQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.430"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>407
                     /locus_tag="OO515_004470"
     CDS             <1..>407
                     /locus_tag="OO515_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578193.1"
                     /translation="FTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNV
                     GSNSERITATPKSGGPSYVYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYL
                     NHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          114 a           84 c          107 g          102 t
ORIGIN      
        1 gttcaccggg gcgtattatc agttgaataa tgacaacttt gccccaggaa aaacggcggc
       61 tgattatgag ttttcaagct ctgcctcctg ggtcgatgtt gatgctaccg gtaaagtgac
      121 atttaaaaat gtcggcagca attcggaaag gattacggcg acaccaaaat caggaggccc
      181 tagctatgta tacgaaatcc gtgtgaagag ttggtgggtg aacgccggcg aggctttcat
      241 gatatacagc cttgctgaaa atttttgcag cagcaatggc tacacgctcc ccagagcaaa
      301 ctatttaaac cacagtagtt cccgaggcat cgggtcactg tacagtgaat ggggagatat
      361 ggggcattac acgactgaag ctggttttca atcaaatatg tattggt
//
LOCUS       DAJTXQ010000425          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000425 DAJTXQ010000000
VERSION     DAJTXQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.431"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..109
                     /locus_tag="OO515_004471"
     CDS             <1..109
                     /locus_tag="OO515_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4223 family protein"
                     /protein_id="HCR6578194.1"
                     /translation="TGHIENRDKNCSYDYLLHPAISISKIIGGCGPTAQ"
BASE COUNT          107 a          101 c           67 g          130 t
ORIGIN      
        1 tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc
       61 aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag
      121 aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg
      181 tttctatgtc cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata
      241 actacatcca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta
      301 cataacatca tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc
      361 aaactctccc ctacaacgta ttggacaaga aaaaggtatc gctat
//
LOCUS       DAJTXQ010000426          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000426 DAJTXQ010000000
VERSION     DAJTXQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.432"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..363)
                     /locus_tag="OO515_004472"
     CDS             complement(<1..363)
                     /locus_tag="OO515_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6578195.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           85 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatacttt
//
LOCUS       DAJTXQ010000427          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000427 DAJTXQ010000000
VERSION     DAJTXQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.433"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            66..>397
                     /gene="yhaV"
                     /locus_tag="OO515_004473"
     CDS             66..>397
                     /gene="yhaV"
                     /locus_tag="OO515_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCR6578196.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          123 a           73 c           99 g          102 t
ORIGIN      
        1 caaggaaaga aacttgtcac tggcatgaac gtcaacattg atgatgagat tggcgacgac
       61 gaataatgga ttttccacaa agggttaatg gttgggcgct atatgctcat ccctgttttc
      121 aggaaaccta cgacgcttta gttgccgaag tcgagacatt aaagggaaaa gatcctgaaa
      181 attatcagag aaaagccgcc acaaagttat tggcggtagt ccataaagtg attgaggagc
      241 atatcacggt caatccatca tcaccggcat tccgtcatgg caagtcgtta ggctctggga
      301 aaaataaaga ctggtcacgg gtaaaatttg gtgcaggtcg ttatcgtctc ttctttcgtt
      361 atagtgaaaa agagaaagtc atcattctgg gatggat
//
LOCUS       DAJTXQ010000428          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000428 DAJTXQ010000000
VERSION     DAJTXQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.434"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>395)
                     /locus_tag="OO515_004474"
     CDS             complement(<1..>395)
                     /locus_tag="OO515_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR6578197.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAG"
BASE COUNT           72 a          137 c          116 g           70 t
ORIGIN      
        1 caccggcggc ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca
       61 gtacccggta cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac
      121 accagccgag cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac
      181 ttaagcggag ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca
      241 gtttcgccac gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac
      301 cgggaaacag gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt
      361 tatcgcgcag ctgtaagcgg atatccgcag gcatt
//
LOCUS       DAJTXQ010000429          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000429 DAJTXQ010000000
VERSION     DAJTXQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.435"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            8..384
                     /gene="rnpB"
                     /locus_tag="OO515_004475"
     ncRNA           8..384
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO515_004475"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT           90 a          106 c          137 g           61 t
ORIGIN      
        1 gcgcgccgaa gctgaccaga cagtcgccgc ttcgtcgtcg tcctcttcgg gggagacggg
       61 cggaggggag gaaagtccgg gctccatagg gcagggtgcc aggtaacgcc tgggggggaa
      121 acccacgacc agtgcaacag agagcaaacc gccgatggcc cgcgcaagcg ggatcaggta
      181 agggtgaaag ggtgcggtaa gagcgcaccg cgcggctggt aacagtccgt ggcacggtaa
      241 actccacccg gagcaaggcc aaataggggt tcataaggta cggcccgtac tgaacccggg
      301 taggctgctt gagccagtga gcgattgctg gcctagatga atgactgtcc acgacagaac
      361 ccggcttatc ggtcagtttc acctgattta cgta
//
LOCUS       DAJTXQ010000430          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000430 DAJTXQ010000000
VERSION     DAJTXQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.436"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>391)
                     /locus_tag="OO515_004476"
     CDS             complement(<1..>391)
                     /locus_tag="OO515_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6578198.1"
                     /translation="GDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAET
                     FTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQ
                     ALTINWYYADVNGNIGYVHTGAYPDRQS"
BASE COUNT           90 a          106 c           82 g          113 t
ORIGIN      
        1 tgattgacga tctggataag caccagtatg aacataacca atattgccgt ttacatcagc
       61 atagtaccag ttgatggtca gtgcttgttt cgctgcctgc tgtgtccact cctgccaatt
      121 tttggccttc atctgatgag tccaggccag caaagacgcc acctctttac catcccatgc
      181 gcgggattta gcgtaagccg tttgtgtcgt ctggtcagtt tggagaatgt tgccatgcac
      241 cgtacgccag acagtaaagg tctctgcctg accatttttc accgtaatgg tttcctcacg
      301 gcttaacatt ttcacccact taccattatg caagtagtag cctggtttct ctgccaacag
      361 ccgttcagca aaaatatcga catcatcgcc g
//
LOCUS       DAJTXQ010000431          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000431 DAJTXQ010000000
VERSION     DAJTXQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.437"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>391
                     /locus_tag="OO515_004477"
     CDS             <1..>391
                     /locus_tag="OO515_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6578199.1"
                     /translation="WSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYD
                     SRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT           93 a          112 c          118 g           68 t
ORIGIN      
        1 tggagccgtt acggtcagtt gctgagcttc accgactgtt ccggttatgt aacccgttat
       61 gaccatgacc gcttcgggca gatgacggcg gtgcaccgcg aggaagggct gagtcagtac
      121 cgcgcatacg acagccgtgg acagttaatt gccgtgaaag acacgcaggg ccatgaaacg
      181 cggtatgaat acaacgccgc cggtgacctg accaccgtca ttgccccgga cggcagcaga
      241 aacgggacac agtacgatgc gtggggaaaa gctatctgta ccacgcaggg cggtctgacg
      301 cgcagtatgg aatacgatgc tgccggacgg gtcatccgcc tgaccagtga aaacggcagc
      361 cacaccacct tccgttacga tgtactcgac c
//
LOCUS       DAJTXQ010000432          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000432 DAJTXQ010000000
VERSION     DAJTXQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.438"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>390
                     /locus_tag="OO515_004478"
     CDS             <1..>390
                     /locus_tag="OO515_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6578200.1"
                     /translation="GQSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRS
                     LNDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAG
                     RINSRSTGSVEGGKSRLSVADAITRVM"
BASE COUNT          103 a           63 c          108 g          116 t
ORIGIN      
        1 tggtcaatct aaaagtcttg gtgaatctct tggtggcttg atgagcgata tgtcgcatgg
       61 ggtcgattca ctaaccgata ttagttacag ggtacgagcg ttctttgatg aggtgacatt
      121 agcttatcgt tcactaaatg acactcaaaa agctgtagct gatggtcttg caaatggcct
      181 gttaagtgca ttaaaggggc ttgctggtat agtcgctgta cggtctggga ttggtgctgt
      241 aggtggtatc tggaacttaa ttagggccat ttccacgctt ggaaatgtgg caaatacagc
      301 agcaggaaga atcaattcac gttctactgg tagtgttgaa ggtggaaaaa gtaggctatc
      361 tgtagctgat gcaattacca gagtaatgat
//
LOCUS       DAJTXQ010000433          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000433 DAJTXQ010000000
VERSION     DAJTXQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.439"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>385)
                     /locus_tag="OO515_004479"
     CDS             complement(<1..>385)
                     /locus_tag="OO515_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HCR6578201.1"
                     /translation="QGAKVEFTVEQLQQSEYLQLPAFITVPPPTLWFVQRRRYFRISA
                     PLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKPAELQEGMRFAQIEVNMGQW
                     GVFHFDAQLISISERKVIDGKNETIT"
BASE COUNT           98 a           89 c          101 g           97 t
ORIGIN      
        1 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
       61 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
      121 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
      181 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
      241 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
      301 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
      361 agtaaactcg actttcgcac cctga
//
LOCUS       DAJTXQ010000434          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000434 DAJTXQ010000000
VERSION     DAJTXQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.440"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..210
                     /locus_tag="OO515_004480"
     CDS             <1..210
                     /locus_tag="OO515_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion system (T7SS), usher family
                     protein"
                     /protein_id="HCR6578202.1"
                     /translation="FGALASNDETGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQ
                     QCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            221..>385
                     /locus_tag="OO515_004481"
     CDS             221..>385
                     /locus_tag="OO515_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6578203.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          113 a           82 c          103 g           87 t
ORIGIN      
        1 tttggcgctc ttgccagcaa cgatgagacg gggcaacaaa gtatcgtcga tgagggcggc
       61 atactatatc tctctgggat atcgagtaaa tcacaaagct ggactgtacg ctggggaaat
      121 caggcagatc aacaatgtca gtttgctttt agtacaccgg attcagaacc aacaacctct
      181 gtattacaag gcacggcgca gtgccattaa ggataaaaaa atgaaaagag cgcctcttat
      241 aacaggactt ttgttgatat ccacatcctg cgcttatgcc tcctcaggag ggtgtggagc
      301 tgacagcact agcggtgcga caaattacag cagtgtggtt gatgatgtta cggtgaacca
      361 gacagataac gtgacaggac gggag
//
LOCUS       DAJTXQ010000435          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000435 DAJTXQ010000000
VERSION     DAJTXQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.441"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          132 a           97 c           51 g          102 t
ORIGIN      
        1 gttccagacg tgaatttgct ggctctcagg gctggcgata taaactgttt gcttcatgaa
       61 cacacctcaa aaatgataaa tcccttatct ttcatacaca taacaacact tactggataa
      121 aaaacagaca acaaaacacg ataaaataca atatcgcaca tcaattaaat caataagtta
      181 gcactccatt tcgaatggca tattactaaa atattagtca ccgacacgaa tttgacaatg
      241 ccacagatat tttcgcgact attcagggag tatgactctc cccgcttttg tttacctgta
      301 tcaacgaccg taaacactat gccataacaa cttctggcca acttcccatt cgctcaacaa
      361 tcaactcact tccggcacaa at
//
LOCUS       DAJTXQ010000436          376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000436 DAJTXQ010000000
VERSION     DAJTXQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.442"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>376)
                     /locus_tag="OO515_004482"
     CDS             complement(<1..>376)
                     /locus_tag="OO515_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6578204.1"
                     /translation="GDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQYPTNPSE
                     SESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDK
                     IFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          146 a           56 c           76 g           98 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctcca
//
LOCUS       DAJTXQ010000437          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000437 DAJTXQ010000000
VERSION     DAJTXQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.443"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..>375
                     /locus_tag="OO515_004483"
     CDS             10..>375
                     /locus_tag="OO515_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6578205.1"
                     /translation="MLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDID
                     AQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYAK
                     AYALFNQAAQHGVNLAWSRL"
BASE COUNT          101 a           73 c          108 g           93 t
ORIGIN      
        1 ttttctgtaa tgctgatagt ttcaccaagc gtggtggcca gcgagaaagc gcatgaattg
       61 tatgacagca tatacggtgg taaacctgct cccgatgtta taaatacact gcataaaatg
      121 gctgaatcgg gagatatcga cgcgcagagc ctgttaggct gggaatatta tcagcctcgt
      181 tatgatacca aacctgatgt tcaggaagcg attaaatggt tcgagttagc ggctaaacaa
      241 ggtgacagag aagctccgtt agcgctgggg ggtatctact acgacggtga gcaggtgcgg
      301 gtggattatg ccaaagcgta tgcactattt aatcaggccg cgcagcacgg tgtgaattta
      361 gcatggtcca ggttg
//
LOCUS       DAJTXQ010000438          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000438 DAJTXQ010000000
VERSION     DAJTXQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.444"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..374)
                     /locus_tag="OO515_004484"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="OO515_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          106 a           88 c           91 g           89 t
ORIGIN      
        1 tgtggtaaac caccgagtgc cgtgccctga aaaaagaaag gtgatgattg ctgactatta
       61 ccgctacgaa aatcgcccgc cgtcaggtca aatttgaaac gcccttcgcg ttgtaaaatc
      121 ggcactgttg aatacggaat tgtgtaattc tgccatcgcg ctcgtcgatg gtgacatcca
      181 gatcgccatt tgaagatgtc gggtgcaaat cagtaatttc aaaagcaccg ggggaaacat
      241 agctttgata gataataaaa ccattttgcc gaatcgttag ctgggccgcc gtacgggcaa
      301 tcccacgtac cgttggggca aacccttgct ggctatctgg atacatatta tcggaagaat
      361 aaagccgtac acca
//
LOCUS       DAJTXQ010000439          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000439 DAJTXQ010000000
VERSION     DAJTXQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.445"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>371
                     /locus_tag="OO515_004485"
     CDS             <1..>371
                     /locus_tag="OO515_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6578206.1"
                     /translation="HYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQ
                     IYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRR
                     YDYLTEYVQVLRDLWGTGKSD"
BASE COUNT           71 a          106 c          101 g           93 t
ORIGIN      
        1 agcattacca tttcgatttc gccctgtcga tgatcaaact gcgtggcttt ggcggcaaaa
       61 ctgagttctg ggatcacaac cttgagtcgt tcaccttgat ggcgggtctg gcagccgtga
      121 cctcgcgcat tcagatttac gccactgctg ccaccttaac gttacctccg gcaatcgtcg
      181 cccgtatggc cgcaaccatc gactccatct ctggcgggcg ttttggcgtc aacctcgtga
      241 ctggctggca aaagcccgtg tatgagcaga tgggtatctg gcctggcgat gactatttct
      301 cccgtcgtta cgactatctc accgaatatg ttcaggtgct gcgcgacctg tggggcacgg
      361 ggaaaagcga t
//
LOCUS       DAJTXQ010000440          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000440 DAJTXQ010000000
VERSION     DAJTXQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.446"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..87
                     /locus_tag="OO515_004486"
     CDS             <1..87
                     /locus_tag="OO515_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6578207.1"
                     /translation="KAREGHSIEQLCATAALAGEAIKTILKE"
     gene            232..>366
                     /gene="tabA"
                     /locus_tag="OO515_004487"
     CDS             232..>366
                     /gene="tabA"
                     /locus_tag="OO515_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6578208.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
BASE COUNT          113 a           77 c           97 g           79 t
ORIGIN      
        1 aaggctcggg aggggcactc catcgaacaa ctctgtgcaa cagctgcact ggcgggggaa
       61 gcgataaaaa ctattctcaa ggagtgatgc gggccttgac ccgaaaggcg ggaacgcgcc
      121 tgccgataag ttgtgataag acaataattc acgcattaag gctagcggaa ttgatcatct
      181 tttcgtataa cgatggaaat gaaacattgt tttaattaag gagtggaaaa gatgatcatc
      241 ggaaatattc ataatcttca gccgtggcta ccccaggagt tacgcctggc gattgagcat
      301 atcaaagcac acgttacggc agaaacgcca aagggcaagc acgatatcga aggcaatcgt
      361 ctgttt
//
LOCUS       DAJTXQ010000441          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000441 DAJTXQ010000000
VERSION     DAJTXQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.447"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            176..>359
                     /locus_tag="OO515_004488"
     CDS             176..>359
                     /locus_tag="OO515_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6578209.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSR"
BASE COUNT          101 a           79 c           66 g          113 t
ORIGIN      
        1 cattacccac aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt
       61 tactcattgt gttttatttc tcacattgat gacgatcgca tgaaaaaatg atattttaat
      121 gagcattctg aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg
      181 tttttctcac cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc
      241 tcaggagcct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
      301 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgtt
//
LOCUS       DAJTXQ010000442          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000442 DAJTXQ010000000
VERSION     DAJTXQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.448"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..>351)
                     /gene="gatA"
                     /locus_tag="OO515_004489"
     CDS             complement(33..>351)
                     /gene="gatA"
                     /locus_tag="OO515_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6578210.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT           99 a           72 c           71 g          109 t
ORIGIN      
        1 ataaatagtc ctttttattg ttacagaggg atttattcgt ctgaatctaa aacatacttt
       61 gtgaagtatt cctttaactg ggtttcagga agagtgatta gtgtctcgac gatatcgggc
      121 tgttgtaact tgccaaataa acagcgtaaa agtttcaatt gctgctgcgg attctccaca
      181 atcaacgcaa taaccaacga tacctccacg tcgttatcat catccgcttg ctgaaaatga
      241 actttatttg ttggccttaa cagataaatg gctgacgact tagcatgaat cgcctcacaa
      301 tgcggtattg caatggcgtg ctgctcaagc attatcccgg tagggaattc t
//
LOCUS       DAJTXQ010000443          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000443 DAJTXQ010000000
VERSION     DAJTXQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.449"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..>349)
                     /locus_tag="OO515_004490"
     CDS             complement(25..>349)
                     /locus_tag="OO515_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6578211.1"
                     /translation="AWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRD
                     VMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNSVR
                     NFIVE"
BASE COUNT           72 a          114 c           72 g           91 t
ORIGIN      
        1 gcatacgacg tgccgcagct tctgttattc cacgataaaa ttcctaaccg aattcaaatc
       61 cttcagacta ttcagctctt ccttcagctc ccgctgacgg cgataaatct cgtcattgcg
      121 ctcaacctgt gcctgagcca ttgctgccgc cagttcttcc agttccggca tcgactgttt
      181 cacctgctga ttatcggcat caccccacgt catcacgtcc cgtacaatgt cggatttcgc
      241 agccattacc accggataaa ggcggcccag tgagttgggg ccagcattcc agatacgacc
      301 gttccattca aacgtgaacg gcttcgcctc ctgttctgtg cgccatgct
//
LOCUS       DAJTXQ010000444          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000444 DAJTXQ010000000
VERSION     DAJTXQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.450"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           68 a          124 c          108 g           47 t
ORIGIN      
        1 acctgagaag aggaaacaga acggggactg gccggggaag ataaggaaga ggcgcgctgt
       61 ttacgggacg cttccgcccg ctgcgcctgc gtgctcagcg ccatgtgaaa acggcggccc
      121 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgccgc ccggtggaaa
      181 gtcagtgagc gcccgaagcc atccaccacg accgtcagta cccggtatgc gggcggctcc
      241 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      301 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc ttagcgg
//
LOCUS       DAJTXQ010000445          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000445 DAJTXQ010000000
VERSION     DAJTXQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.451"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           97 a           70 c           48 g          124 t
ORIGIN      
        1 gaatatctca atttttaaac ataaaatgcg aattatttag tacaaaaagt gaacttctga
       61 tgatctttcc ctggcctgcc accatctctt cacaagctta tgcttattac atgttacatg
      121 caaaacgtcc cgttttgcat ctttgtgcct acggcacacc gtgatctttc cctgatgagg
      181 gatatattaa ctattgaaca ttagcgcgaa ttatttagtg caaaaaatca gcatcacgtg
      241 atgcttttcc tgctattaat aacctcctct atttaactat atttttcatt gatgcaattc
      301 gtagatttga agaagttcaa ctgccacttt tttattttt
//
LOCUS       DAJTXQ010000446          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000446 DAJTXQ010000000
VERSION     DAJTXQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.452"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..263)
                     /locus_tag="OO515_004491"
     CDS             complement(<1..263)
                     /locus_tag="OO515_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6578212.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           88 a           84 c           66 g          100 t
ORIGIN      
        1 gtgtctggtt cttccggcca ctcaatatca ggtgcagttg atgtatcaac acggttcagc
       61 aacacccgat acttcttcca ggcttccagc aacgagtttt cttcctccgt tgcgatctcc
      121 agatctacag catcctgaag tggcgcaata tgctcactga attcctggat gtagaactgt
      181 gtggtgacgg tcttccagcc attcggctcc tgctgtatcg aagcatacca ggctatttca
      241 atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat
      301 tcacaaataa aactataata tggtgaaatt aatgaaaa
//
LOCUS       DAJTXQ010000447          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000447 DAJTXQ010000000
VERSION     DAJTXQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.453"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>336)
                     /locus_tag="OO515_004492"
     CDS             complement(<1..>336)
                     /locus_tag="OO515_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6578213.1"
                     /translation="KIKPMLVVIPDTETDAKGIIPEDFVPQERRKVFYPLNAKAADRE
                     LMNDIIPLISKRFNVRKDADGRALAGLSQGGYQALVSGMNHLESFGWLATFSGVTTTT
                     VPDEGVAARL"
BASE COUNT           77 a           97 c           79 g           83 t
ORIGIN      
        1 agccgggccg cgacaccttc atccggtacg gttgtcgtgg taacaccact gaatgtggcc
       61 agccagccaa agctttccag atgattcatt ccggaaacca gcgcctggta cccgccttgt
      121 gaaagccctg ccagcgcgcg accatcggca tctttacgga cattaaaacg cttgctaatc
      181 agcgggataa tatcgttcat cagttcgcga tctgccgctt tagcattcag cggataaaag
      241 actttacgtc tttcctgagg cacgaaatct tcgggaataa tgcccttcgc atcggtttcg
      301 gtatccggga tcaccaccag catcggttta attttc
//
LOCUS       DAJTXQ010000448          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000448 DAJTXQ010000000
VERSION     DAJTXQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.454"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>330
                     /locus_tag="OO515_004493"
     CDS             <1..>330
                     /locus_tag="OO515_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="activator of genes involved in phenylacetic acid
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCR6578214.1"
                     /translation="VCAERLDADLPMVQLSHRLLQESMNNPALSETESEAALQAMVCL
                     LRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADK
                     GLVVAQY"
BASE COUNT           87 a           71 c           91 g           81 t
ORIGIN      
        1 ctgtctgcgc agaaagactg gacgctgact tacccatggt gcaactcagt catcgcctgt
       61 tacaggagag catgaataat ccggcacttt ctgaaacaga aagtgaagct gcgctacagg
      121 cgatggtgtg tctgctgcgc ccggtacttc atcagcggga atctgttcaa cctcgccgtg
      181 aacgtcagtt tcaaaaagtg gttacgttga tagacgataa tattcgcgaa gagatattac
      241 gcccggagtg gatagccgga gagacaggta tgtcagtacg tagtttgtac cgaatgtttg
      301 ccgataaagg tttggtagtc gcgcaatata
//
LOCUS       DAJTXQ010000449          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000449 DAJTXQ010000000
VERSION     DAJTXQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.455"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>330
                     /locus_tag="OO515_004494"
     CDS             <1..>330
                     /locus_tag="OO515_004494"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6578215.1"
                     /translation="GMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGI
                     LGGIPQSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHF
                     DSAWVPYT"
BASE COUNT           86 a           97 c           69 g           78 t
ORIGIN      
        1 tggcatgtac tctgctccgg caggcagcac cattctgatt gaccgtaact gccacaaatc
       61 gctgacccac ctgatgatga tgagcgatgt tacgccaatc tatttccgcc cgacccgtaa
      121 cgcttacggt attcttggtg gtatcccaca gagtgaattc cagcacgcta ccattgctaa
      181 gcgcgtgaaa gaaacaccaa acgcaacctg gccggtacat gctgtaatta ccaactctac
      241 ctatgatggt ctgctgtaca acaccgactt catcaagaaa acactggatg tgaaatccat
      301 ccactttgac tccgcgtggg tgccttacac
//
LOCUS       DAJTXQ010000450          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000450 DAJTXQ010000000
VERSION     DAJTXQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.456"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            18..213
                     /locus_tag="OO515_004495"
                     /pseudo
     CDS             18..213
                     /locus_tag="OO515_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           91 a           38 c           96 g          104 t
ORIGIN      
        1 cggcggggag agatttagtg ctgtgaaata ttgtggtact gatgaatatt tatatcgcgg
       61 attggtagag aaaagattat ttcatgcact atttttgcaa atatttctat atgggaagcg
      121 tgtttgttac ggattaattc ataaagaaat gcatttgggt gttggtttga agagtcgctg
      181 gttgatgtta ttgtgccagg aggggcaggg taaggtgctg tatcgatatg cattggcaat
      241 tgcgtgtgcc gcagatggag atgaaatgcc aataaaaaag cctgaattcg atgagggacg
      301 aactcaggct agtgtttagt tttacagta
//
LOCUS       DAJTXQ010000451          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000451 DAJTXQ010000000
VERSION     DAJTXQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.457"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>329
                     /locus_tag="OO515_004496"
     CDS             <1..>329
                     /locus_tag="OO515_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6578216.1"
                     /translation="DVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKND
                     RFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWYFGEELK
                     KMGMNII"
BASE COUNT           79 a           85 c           82 g           83 t
ORIGIN      
        1 gatgttgtcg ccagcctcaa cgctgatagc gaatatgcag caatctttgt tcctggtggt
       61 catggcgcac ttattggttt accagaaagc caggacgtag ctgctgcttt gcagtgggca
      121 atcaaaaatg accgttttgt tatctccctt tgccacggcc cggcggcttt tctggcgctt
      181 cgccacggcg ataacccact gaatggttat tccatttgcg cattcccaga cgccgcagac
      241 aaacaaacgc cagagatagg ctatatgccg ggtcatctca cctggtactt cggcgaagag
      301 ctgaagaaaa tgggcatgaa cattattaa
//
LOCUS       DAJTXQ010000452          328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000452 DAJTXQ010000000
VERSION     DAJTXQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.458"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>328
                     /locus_tag="OO515_004497"
     CDS             <1..>328
                     /locus_tag="OO515_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6578217.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRD"
BASE COUNT          113 a           58 c           89 g           68 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagattt
//
LOCUS       DAJTXQ010000453          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000453 DAJTXQ010000000
VERSION     DAJTXQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.459"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>325
                     /locus_tag="OO515_004498"
     CDS             <1..>325
                     /locus_tag="OO515_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6578218.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPD"
BASE COUNT          111 a           64 c           72 g           78 t
ORIGIN      
        1 attaagcaca tcatcatagc tgagagagca caccaagaat ctcttgttaa gcaaaagcgt
       61 atagctcagg tctggaacca taaaacacag cttgcaagag aacttaagaa gcctatgggt
      121 aagcaagccc ccggatggtt agagctatca gaagatggtt catactacat cgttgatgaa
      181 gataaagcgt cactggtgaa tatcatctat gacaagcgtc tttctggtat gtcgatgttt
      241 gctatctgta aatggctaaa cgaacaaggt tacctgacca ttaatcagcg taaggtcaga
      301 atatccaaga ctaagaaacc ggatg
//
LOCUS       DAJTXQ010000454          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000454 DAJTXQ010000000
VERSION     DAJTXQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.460"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>322
                     /locus_tag="OO515_004499"
     CDS             <1..>322
                     /locus_tag="OO515_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6578219.1"
                     /translation="VQIRQNQPHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVN
                     GHAHLLYALNIAVRTAPDSSVKALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQ
                     VMEWR"
BASE COUNT           73 a           84 c           95 g           70 t
ORIGIN      
        1 atgtccagat ccggcagaac cagcctcatg cgcagttctg gctggttttt gacgttgatc
       61 gcgagggggc agcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtaa
      121 aaaatcctgt gaacggtcat gctcacctgc tctacgcgct caatatcgcc gtgagaaccg
      181 cgccggacag ctcggttaag gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg
      241 agaaactttg tgctgatgtg aactacagcg gactgatttg taaaaatccg ttccacctgg
      301 aatggcaggt gatggagtgg cg
//
LOCUS       DAJTXQ010000455          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000455 DAJTXQ010000000
VERSION     DAJTXQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.461"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           91 a           69 c           61 g          101 t
ORIGIN      
        1 ggtaatgttc atggttcctc cttatcgttc ccttaagaat actgcgtgag ataatagtga
       61 gaagcggagc actctgaaag gaggaagtcg tatttcctta accgccactc cgcaagcctg
      121 acatatcttt taaggaaatg ctgtagataa acattattca tcttgcagca gagaattgta
      181 tttttctgaa ttcctgaatt tttaccctta ccgttttaga attaataaaa aacccggcgc
      241 taaatgctta ccgggttttt tctttatcag gcaaatagca gggttaaccg ctaatctgct
      301 ccatcgcctg caaaatacgc tt
//
LOCUS       DAJTXQ010000456          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000456 DAJTXQ010000000
VERSION     DAJTXQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.462"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            132..>321
                     /locus_tag="OO515_004500"
     CDS             132..>321
                     /locus_tag="OO515_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6578220.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPT"
BASE COUNT          100 a           80 c           57 g           84 t
ORIGIN      
        1 attacatact tacacccgcc gactaccgct ttcgatatat tgactcaatt ttccagactg
       61 gttaaaaata gcgaccagct cagtcgcaaa tatagtgact accctaacta agcaacaata
      121 aggaatacac tatgactgtt caaacaagta aaaatccgca ggtcgatatt gctgaagata
      181 atgcattctt cccttcagaa tattcgctta gccaatatac cagtcccgtc tctgatcttg
      241 atggcgtgga ctatccaaaa ccgtatcgcg gtaagcataa aattctggtg atcgccgcgg
      301 acgaacgtta tttgccaacc g
//
LOCUS       DAJTXQ010000457          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000457 DAJTXQ010000000
VERSION     DAJTXQ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.463"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..146
                     /locus_tag="OO515_004501"
     tRNA            71..146
                     /locus_tag="OO515_004501"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAJTXQ010000458          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000458 DAJTXQ010000000
VERSION     DAJTXQ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.464"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..164
                     /locus_tag="OO515_004502"
     CDS             <1..164
                     /locus_tag="OO515_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6578221.1"
                     /translation="NLAFLTAFMDRSALPFKKAIPELAWGSYAWFASEPEHLIDLRDG
                     YPVKISQRK"
BASE COUNT           89 a           61 c           74 g           88 t
ORIGIN      
        1 aaaatcttgc attccttacg gcatttatgg acagaagtgc tctgcctttt aaaaaggcaa
       61 taccagaatt ggcgtgggga tcttatgcat ggtttgcttc tgaaccggag cacttaattg
      121 atcttcggga tggttatccg gtaaagatca gccagagaaa atgaccaaag tttgatatcc
      181 gccacgccct gaaggatggg ggttttacgg tgcactggat aaaaaatcgg tttcatagat
      241 ttgcccaccg tggtatacgc ctgcgataaa aactttattg tcatcaacgg caaaagcaat
      301 aattgttctg tg
//
LOCUS       DAJTXQ010000459          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000459 DAJTXQ010000000
VERSION     DAJTXQ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.465"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>308
                     /locus_tag="OO515_004503"
     CDS             <1..>308
                     /locus_tag="OO515_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6578222.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJTXQ010000460          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000460 DAJTXQ010000000
VERSION     DAJTXQ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.466"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..305
                     /locus_tag="OO515_004504"
                     /pseudo
     CDS             1..305
                     /locus_tag="OO515_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          100 a           59 c           75 g           71 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttac
//
LOCUS       DAJTXQ010000461          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000461 DAJTXQ010000000
VERSION     DAJTXQ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.467"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT           94 a           63 c           64 g           84 t
ORIGIN      
        1 ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa atgcagaacc
       61 cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc gagtatccat
      121 tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac gcacacacaa
      181 acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc tgccgcttcg
      241 ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta cttcaccatc
      301 ggagt
//
LOCUS       DAJTXQ010000462          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.468, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000462 DAJTXQ010000000
VERSION     DAJTXQ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.468"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>304
                     /locus_tag="OO515_004505"
     CDS             <1..>304
                     /locus_tag="OO515_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6578223.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR"
BASE COUNT           52 a           67 c           88 g           97 t
ORIGIN      
        1 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
       61 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
      121 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
      181 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
      241 ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
      301 cgcg
//
LOCUS       DAJTXQ010000463          302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000463 DAJTXQ010000000
VERSION     DAJTXQ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.469"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>302
                     /locus_tag="OO515_004506"
     CDS             <1..>302
                     /locus_tag="OO515_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6578224.1"
                     /translation="VKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTDNN
                     GAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV"
BASE COUNT           70 a           63 c           98 g           71 t
ORIGIN      
        1 gtaaagattt caggtgtact gaaagatggg gcgggtaaac ctgtcgtaaa ttgtgcgatt
       61 gaactgcggg ccagaagaac cagtccgacc gttgtggcac acgttgttgc cacttgcgtg
      121 acggacaata acggtgctta tgtgattgag gctgagccgg ggtattacga ggttgcgctt
      181 cactgtaacg gctggcagcc aacccgtgtc ggggatattg atgtggcacc gactgatgca
      241 ccggggacac tgaacgcgtt tctgaatgca ccaaaggatg gtgatttacg tccggaggtg
      301 at
//
LOCUS       DAJTXQ010000464          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000464 DAJTXQ010000000
VERSION     DAJTXQ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.470"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..>301
                     /locus_tag="OO515_004507"
     CDS             115..>301
                     /locus_tag="OO515_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6578225.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYG"
BASE COUNT           77 a           73 c           71 g           80 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 a
//
LOCUS       DAJTXQ010000465          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000465 DAJTXQ010000000
VERSION     DAJTXQ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.471"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            112..>301
                     /locus_tag="OO515_004508"
     CDS             112..>301
                     /locus_tag="OO515_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578226.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSY"
BASE COUNT           95 a           65 c           78 g           63 t
ORIGIN      
        1 atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat tgaagcacag
       61 agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa gatggatatt
      121 gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa attgcatgaa
      181 tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt aaagcagtta
      241 acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc gatgagttat
      301 c
//
LOCUS       DAJTXQ010000466          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.denovo.472, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000466 DAJTXQ010000000
VERSION     DAJTXQ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.denovo.472"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>301
                     /locus_tag="OO515_004509"
     CDS             <1..>301
                     /locus_tag="OO515_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6578227.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAJTXQ010000467         6278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.guided.187, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000467 DAJTXQ010000000
VERSION     DAJTXQ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.guided.187"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..683)
                     /gene="epmA"
                     /locus_tag="OO515_004510"
     CDS             complement(<1..683)
                     /gene="epmA"
                     /locus_tag="OO515_004510"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6578228.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQIST"
     gene            1008..2816
                     /gene="frdA"
                     /locus_tag="OO515_004511"
     CDS             1008..2816
                     /gene="frdA"
                     /locus_tag="OO515_004511"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6578229.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2809..3543
                     /gene="frdB"
                     /locus_tag="OO515_004512"
     CDS             2809..3543
                     /gene="frdB"
                     /locus_tag="OO515_004512"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6578230.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3554..3949
                     /gene="frdC"
                     /locus_tag="OO515_004513"
     CDS             3554..3949
                     /gene="frdC"
                     /locus_tag="OO515_004513"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6578231.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3960..4319
                     /gene="frdD"
                     /locus_tag="OO515_004514"
     CDS             3960..4319
                     /gene="frdD"
                     /locus_tag="OO515_004514"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6578232.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4382..5515
                     /locus_tag="OO515_004515"
     CDS             4382..5515
                     /locus_tag="OO515_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6578233.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            5604..6137
                     /gene="blc"
                     /locus_tag="OO515_004516"
     CDS             5604..6137
                     /gene="blc"
                     /locus_tag="OO515_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6578234.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6134..>6278)
                     /gene="sugE"
                     /locus_tag="OO515_004517"
     CDS             complement(6134..>6278)
                     /gene="sugE"
                     /locus_tag="OO515_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6578235.1"
                     /translation="YAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         1538 a         1638 c         1669 g         1433 t
ORIGIN      
        1 tcggtactga tttgcgccag tgatgcctgg ctggctggaa agtggtacac aaaggtcggt
       61 ttttctttgc caatatttgg ctctacgcca aaggtaaaca gcaattgtag caacgtgtcg
      121 cggtcttctt cggtatcagc aacgttactc aaatccagtt tcgctgcgac ttcccgcagt
      181 tgcgttttgt cggcagagag cgggtcaatt tccagataac gcaagaaagc ttgttgataa
      241 gaaaggcttt ctgctgccgg gcagtccagc acctgttgta agagatcgtc cacctcgttc
      301 atcaaccggt acatatcata gtgcggtcga taccactcaa gcatagtgaa ctcagggttg
      361 tgataacgcc ccatctcttc attacggaag ctgcggcaca gctggaatac cggcccacaa
      421 ccggcaacca gcaggcgttt catatggtat tccgggctgg tcattaacca gagattcatc
      481 ccctgcgaat gcccggggcc aacgaaacgt gtctcaaacg ggaccaaatg aatatcggtt
      541 accgtcgcct ggctcataca aggcgtctcc acctccagca ctccacgatc ggcaaagaaa
      601 cgacggatct ccgccataat cgccgcacgt tttaataagt taggaatgga tgcgctcggc
      661 tgccaggatg ccgtttcgct catagttaaa tctccagttt ttgacaaggg cacgaagtct
      721 actcgcaacg cgacggcgag acaaatttta cgcaggaatc aaacagcggt gggcagtgac
      781 taaaaaaagc acgatctgat ggtttagtaa ttaaattaat catcttcagt gataatttag
      841 ccctcttgcg cactaaaaaa atcgatctcg tcaaatttca gacttatcca tcagactata
      901 ctgttgtacc tataaaggag cagtggaata gcgttcgcag accgtaactt tcaggcactt
      961 accctgaagt acggggctgt gggataaaaa caatctggag gaatgtcgtg caaacctttc
     1021 aagccgatct tgccattgta ggcgccggtg gcgcgggatt acgtgctgca attgctgccg
     1081 cgcaggcaaa tccaaatgca aaaatcgcac taatctcaaa agtatacccg atgcgtagcc
     1141 ataccgttgc tgcagaaggg ggctccgccg ctgtcgcgca ggatcatgac agcttcgaat
     1201 atcactttca cgatacagta gcgggtggcg actggttgtg tgagcaggat gtcgtggatt
     1261 atttcgtcca ccactgccca accgaaatga cccaactgga actgtggggg tgcccatgga
     1321 gccgtcgccc ggatggtagc gtcaacgtac gtcgcttcgg cggcatgaaa atcgagcgca
     1381 cctggttcgc cgccgataag accggcttcc atatgctgca cacgctgttc cagacctctc
     1441 tgcaattccc gcagatccag cgttttgacg aacatttcgt gctggatatt ctggttgatg
     1501 atggtcatgt tcgcggcctg gtagcaatga acatgatgga aggcacgctg gtgcagatcc
     1561 gtgctaacgc ggtcgttatg gctactggcg gtgcgggtcg cgtttatcgt tacaacacca
     1621 acggcggcat cgttaccggt gacggtatgg gtatggcgct aagccacggc gttccgctgc
     1681 gtgacatgga attcgttcag tatcacccaa ccggtctgcc aggttccggt atcctgatga
     1741 ccgaaggctg ccgtggtgaa ggcggtattc tggtcaacaa aaatggctac cgttatctgc
     1801 aagattacgg catgggcccg gaaactccgc tgggcgagcc gaaaaacaaa tatatggaac
     1861 tgggtccacg cgacaaagtt tctcaggcct tctggcacga atggcgtaaa ggcaacacca
     1921 tctccacgcc gcgtggcgat gtggtttatc tcgacttgcg tcacctcggc gagaaaaaac
     1981 tgcatgaacg tctgccgttc atctgcgaac tggcgaaagc gtacgttggc gtcgatccgg
     2041 ttaaagaacc gattccggta cgtccgaccg cacactacac catgggcggt atcgaaaccg
     2101 atcagaactg tgaaacccgc attaaaggtc tgttcgccgt gggtgaatgt tcctctgttg
     2161 gtctgcacgg tgcaaaccgt ctgggctcca actccctggc ggaactggtg gtcttcggcc
     2221 gtctggccgg tgaacaagcg acagagcgtg cagcaactgc cggtaatggc aacgaagcgg
     2281 caattgaagc gcaggcagct ggcgttgaac aacgtctgaa agatctggtt aaccaggatg
     2341 gcggcgaaaa ctgggcgaag atccgcgacg aaatgggcct ggcaatggaa gaaggttgcg
     2401 gtatctaccg tacgccggaa ctgatgcaga aaaccatcga caagctggca gagctgcagg
     2461 aatgcttcaa gcgcgtgcgc atcaccgaca cctccagcgt gttcaacacc gacctgctct
     2521 acaccattga actgggccac ggtctgaacg ttgctgaatg tatggcgcac tccgcaatgg
     2581 cacgtaaaga gtcccgcggc gcacaccagc gtctggacga aggttgcacc gagcgtgacg
     2641 acgtcaactt cctcaaacac accctcgcct tccgcgatgc tgatggcacg actcgcctag
     2701 agtacagcga cgtgaagatt actacgctgc cgccagctaa acgcgtttac ggtggcgaag
     2761 cggatgcagc cgataaggcg gaagcagcca ataagaagga gaaggcgaat ggctgagatg
     2821 aaaaacctga aaattgaggt ggtgcgctat aacccggaag tcgataccgc accgcatagc
     2881 gcattctatg aagtgcctta tgacgcaact acctcattac tggatgcgct ggtctacatc
     2941 aaagacaacc tggcaccgga cctgagttac cgctggtcct gccgtatggc gatttgtggt
     3001 tcctgcggca tgatggttaa caacgtgcca aaactggcat gtaaaacctt cctgcgtgat
     3061 tacaccgacg gtatgaaggt tgaagcgtta gctaacttcc cgattgaacg cgatctggtg
     3121 gtcgatatga cccacttcat cgaaagtctg gaagcgatca aaccgtacat catcggcaac
     3181 tcccgcaccg cggatcaggg tactaacatc cagaccccgg cgcagatggc aaagtatcac
     3241 cagttctccg gttgcatcaa ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg
     3301 aacccagagt tcatcggtcc ggctgccatt acgctggcgc atcgttataa cgaagatagc
     3361 cgcgaccacg gtaagaagga gcgtatggcg cagttgaaca gccagaacgg cgtatggagc
     3421 tgtactttcg tgggctattg ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc
     3481 attcagcagg gcaaagtaga aagttcgaaa gactttctta tcgcgaccct gaaaccacgc
     3541 taaggagtgc aacatgacga ctaaacgtaa accgtatgta cggccaatga cgtccacctg
     3601 gtggaaaaaa ttgccgtttt atcgctttta catgctgcgc gaaggcacgg cggttccggc
     3661 tgtgtggttc agcattgaac tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc
     3721 ctgggcggga ttcgtcgact ttttacaaaa cccggttatc gtgatcatta acctgatcac
     3781 tctggcggca gctctgctgc acaccaaaac ctggtttgaa ctggcaccga aagcggccaa
     3841 tatcattgta aaagacgaaa aaatgggacc agagccaatt atcaaaagtc tctgggcggt
     3901 aactgtggtt gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga
     3961 tgattaatcc aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg
     4021 gtggtatgtg gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc
     4081 cactggggct gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga
     4141 gcttcattgg tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac
     4201 accgtatgca ccacgcgatg cacgatctga aaattcacgt gcctgcgggc aaatgggttt
     4261 tctacggtct ggctgctatc ctgacagttg tcacgctgat tggtatcgtt acaatctaac
     4321 gtatcgccaa tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct
     4381 tatgttcaaa acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc
     4441 ccctcaacaa atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa
     4501 gatccccggt atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg
     4561 ctatgcggac atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc
     4621 ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa
     4681 gttaagcgat cccgcaacaa aatactggcc tgaacttact gctaaacagt ggaatgggat
     4741 cacactatta catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga
     4801 agtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggctcc
     4861 aggaacacaa cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa
     4921 gccgtctggt ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact
     4981 caaccatacg tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg
     5041 cgaaggtaag gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa
     5101 gtcgaccatt gaagatatgg cccgctgggt gcaaagcaat ttaacccccc ttgatatcaa
     5161 tgagaaaacg cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga
     5221 tatgtatcag ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat
     5281 taacggcagt gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc
     5341 aactcctgca gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag
     5401 ctatgtcgcg tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta
     5461 tcccaatcca gcgagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt
     5521 catcgggtcc gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga
     5581 aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg
     5641 catttctggt cgttgcctgc agttctccta cgccgccgcg tggcgtgaca gtagtgaata
     5701 atttcgacgc taaacgctat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt
     5761 ttgaacgtgg actggaaaaa gtcaccgcaa catacagtct gcgtgatgac ggcggcctga
     5821 atgtcatcaa taaaggctat aaccctgaca gagaaatgtg gcagcagagt gaagggaaag
     5881 cgtactttac cggcgcacca acacgcgctg cgctgaaagt gtcattcttt ggtcctttct
     5941 atggcggtta taacgtcatt gcacttgatc gggaataccg ccatgcgctg gtttgcggcc
     6001 cggaccgcga ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac
     6061 aggagatgct ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac
     6121 agcagcccgg tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg
     6181 ccagactcgc caggcgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg
     6241 ccgcgccgac tgcgccaata cccgtccaca cggcataa
//
LOCUS       DAJTXQ010000468         5602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.guided.199, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000468 DAJTXQ010000000
VERSION     DAJTXQ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.guided.199"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..550)
                     /locus_tag="OO515_004518"
     CDS             complement(<1..550)
                     /locus_tag="OO515_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCR6578236.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGG"
     gene            811..1269
                     /gene="gpt"
                     /locus_tag="OO515_004519"
     CDS             811..1269
                     /gene="gpt"
                     /locus_tag="OO515_004519"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6578237.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1361..2605
                     /gene="frsA"
                     /locus_tag="OO515_004520"
     CDS             1361..2605
                     /gene="frsA"
                     /locus_tag="OO515_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6578238.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2663..3064
                     /gene="crl"
                     /locus_tag="OO515_004521"
     CDS             2663..3064
                     /gene="crl"
                     /locus_tag="OO515_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6578239.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3103..4152)
                     /gene="phoE"
                     /locus_tag="OO515_004522"
     CDS             complement(3103..4152)
                     /gene="phoE"
                     /locus_tag="OO515_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6578240.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            4440..5543
                     /gene="proB"
                     /locus_tag="OO515_004523"
     CDS             4440..5543
                     /gene="proB"
                     /locus_tag="OO515_004523"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6578241.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         1368 a         1388 c         1419 g         1427 t
ORIGIN      
        1 taccccccgc acaacccatg tagatttcac cttcttcttc ggagtcggtg ttaatcagaa
       61 tatcagcctg caaccagttg ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg
      121 tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca
      181 gcgcagacgc cataccaatg ccgttatctg cacccagcgt ggtgccgcgc gctttaaccc
      241 attcgccatc aatataaggc tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat
      301 ttttctgcgg caccatatcg aggtgggcct gtaagacgac cgatttacga ttttccatac
      361 ctgcggtagc aggtttacga atcaggatat tacctacctg atcgcgttcg acatggaaac
      421 ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga taggacgggt
      481 gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat aattgagaca
      541 gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg
      601 ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtttgag aataccactt
      661 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
      721 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
      781 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
      841 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
      901 ggcattattg ccgtaagccg tggcggtctg gtaccgggtg cgttactggc gcgtgaactg
      961 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     1021 cttaaagtgc taaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     1081 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     1141 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     1201 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     1261 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     1321 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     1381 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     1441 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     1501 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gtgaaatcct
     1561 cgacgtccag gcgcgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     1621 tacggtgatt ggctaccgtg gtggcaactg gatttatgag tgggccatcc aggcgatggt
     1681 gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc agtggtcgtc actggctgca
     1741 tgcggctacg ttgtacaaca ttgctgccta tcctcatctg aaaggagatg acctggccga
     1801 gcaagcgcag gctttgtcaa accgtgccta tgaagaagcc gcccagcgtc tgccgggcac
     1861 gatgcggcag atggagttta ccgtacccgg cggtgcgccc atcaccggct ttttgcatat
     1921 gccgaaaggc gatggcccgt tcccgacagt attaatgtgt ggtggtctgg atgcgatgca
     1981 gacggactat tacagcctgt atgaacgtta ttttgcgccg cgcggcattg cgatgctgac
     2041 tattgatatg ccgtcggtgg gcttttcatc aaaatggaag ctcacccagg actccagcct
     2101 gttgcatcag cacgttttaa aggctctgcc taacgtaccg tgggtggatc acactcgcgt
     2161 cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg cgtctggcat atcttgaatc
     2221 gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt cataccctgt tgagtgattt
     2281 taagtgccag caacaggtgc cggaaatgta tcttgacgtt ctggcgagtc gtttggggat
     2341 gcatgatgct tccgacgaag cgttacgcgt ggagctgaat cgctattcat taaaagtgca
     2401 aggattgctg gggcgtcgct gcccaacgcc aatgttatca ggctactgga agaacgatcc
     2461 gttcagcccg gaagaggact cacgcttaat cacctcatca tctgctgacg gtaaattatt
     2521 agagatccca tttaacccgg tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg
     2581 ctggatcgaa aaacgcttgt gttaaaaatt tgctaaaatt tgccaatttg gtaaaacagt
     2641 tgcatcacaa caggagattg caatgacgtt accgagtgga cacccgaaga gcagattgat
     2701 caaaaaattt accgcactag gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt
     2761 ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgagtt
     2821 ctggggctgg tggatggagc ttgaagcgca ggaatcccgt tttacctaca gttaccagtt
     2881 tggtctgttc gataaagcag gcgactggaa gagtgttccg gtaaaagaca ctgaagtggt
     2941 tgaacgactg gagcacaccc tacgtgagtt tcacgagaag ctgcgtgaac tgctgacgac
     3001 gctgaatctg aagctggaac cggcggatga ttttcgtgat gaaccggtga agttaacggc
     3061 gtgagtgaaa tgtgccggat gtatcacatc cggcaatatc cattaaaact gataggtcat
     3121 gccaaccgcg acaatatcat cattattaat attcaatttg ttatcgctat ccagttggtt
     3181 gattttataa tcaacaaacg ctgacatatt tttgttgaaa taatacgtag caccgacgtc
     3241 gatataattg accagatctt catcaccgat accttcaata tctttccctt tcgataagac
     3301 ataacccagc gatggacgca gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt
     3361 ctgtgtctta ttggcaaagc cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa
     3421 agttgccaga taaatattat tggcatcgta tttcagacct gttgcccatg cttctgcacg
     3481 cttgcctgtg ccacggcttt gcaggttctg ctcgttggtg cgatctgagt tggtataggc
     3541 cccactaatg gcgaaatcgc tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc
     3601 gccgttttgc tttttaacgt cgcggttttc gtttttccct tgatattgca gggttaagtt
     3661 caggccatcg ataacgccga agaagtcggt gttccgatac gtcgccagac cgctggcgcg
     3721 tttggtcata aagttgtcgg tctgcgccga ggaatcgcca ccaaattccg ggaacatatc
     3781 ggtccaggct tccacgtcat acaacgcgcc caggttacga ccataatcga aagaacccaa
     3841 atctttatat ttcaacccgg caaaagcgag acgcgttttc tgctgtgcag tatcactctc
     3901 cgctttattt ccggcaaact ccgcttccca ccgaccataa ccagtcagtt gatcgttaat
     3961 ttgtgtttca cctttaaaac caaaacggat ataactctgg tcgccatctt tactggcgtt
     4021 atcactcata taatgcataa ctttgccata gacatccagt ttattaccgt ctttattata
     4081 tatttctgca gcctgtacag atgcagatgc cacaatgccc atcaccacta atgccagagt
     4141 gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc
     4201 cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca gattaataat
     4261 attcttaaaa tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctagg
     4321 cttgctgatc ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga
     4381 cagtcctgct aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca
     4441 tgagtgacag ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc
     4501 gccgtctgaa ccgtgcccat attgttgaac ttgttcgcca gtgcgcgcag ttacatgccg
     4561 ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg
     4621 gttacccgga actgccagcg actatcgcct cgaaacaact gctggcagcg gtagggcaga
     4681 gtcgactgat tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa
     4741 tgctgctgac ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc
     4801 tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta
     4861 cggcagagat taaagtcggc gataacgata acctttctgc gctggcggcg attctggcgg
     4921 gtgccgataa actgttgctg ctgaccgatc aaaaaggttt gtataccgct gacccgcgca
     4981 gcaatccgca ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga
     5041 ttgccggtga cagcgtttca ggcctcggaa ctggcggcat gagtaccaaa ttgcaggccg
     5101 ctgacgtggc ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg
     5161 ttattggtga tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc
     5221 cgcttgaaaa ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag
     5281 atgaaggggc aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta
     5341 aaagcgtgac tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggcc
     5401 gcgatatcgc ccacggcgtc agtcgttaca acagcgatgc attacgccgt attgccggac
     5461 accactcgca agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc
     5521 gtgatgacat gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgctgc
     5581 gaagcaagcc tcgtataaat ta
//
LOCUS       DAJTXQ010000469         4956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.guided.208, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000469 DAJTXQ010000000
VERSION     DAJTXQ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.guided.208"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            452..691
                     /locus_tag="OO515_004524"
     CDS             452..691
                     /locus_tag="OO515_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6578242.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(879..1856)
                     /gene="intI2"
                     /locus_tag="OO515_004525"
                     /pseudo
     CDS             complement(879..1856)
                     /gene="intI2"
                     /locus_tag="OO515_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2188..2661
                     /locus_tag="OO515_004526"
     CDS             2188..2661
                     /locus_tag="OO515_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6578243.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2756..3280
                     /gene="sat2"
                     /locus_tag="OO515_004527"
     CDS             2756..3280
                     /gene="sat2"
                     /locus_tag="OO515_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6578244.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3338..4126
                     /gene="aadA1"
                     /locus_tag="OO515_004528"
     CDS             3338..4126
                     /gene="aadA1"
                     /locus_tag="OO515_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6578245.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4202..4699
                     /locus_tag="OO515_004529"
     CDS             4202..4699
                     /locus_tag="OO515_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578246.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4760..>4956
                     /locus_tag="OO515_004530"
     CDS             4760..>4956
                     /locus_tag="OO515_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578247.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLT"
BASE COUNT         1395 a         1114 c         1147 g         1300 t
ORIGIN      
        1 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
       61 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttaa ctaatctagc
      121 taacctgccg ctcagcttag tacgatccta tatccaactg atcataatcc tttcatcagt
      181 atcccaaatt aaatccccct cctagactgc ctcaccaaca ctttacagtg atcccgtatt
      241 tccggacagg tgattgattt ttttcgcttg tacaggtgtt aagccacagt tatcttgatg
      301 tggacattat gcattgtaat agcccattaa catgcgctaa gagcataaat taatatacag
      361 taacaagcat tacgaggtgc taatgtttgg aaagcagcct cttccaacta caataataag
      421 ttaactatcc tccacccaca aataattaca aatgacccct ttccgctata actccgatct
      481 caccagcggc tcgctacaaa cccgagagtg tcgcattatt actggcttgt tactccaaga
      541 gcttgatgaa gctgcctggg ataaagccat gtataaggaa aatgtgcttc agaaacgcac
      601 gcaatctacg gtaagacgta tttcttcagc gcttagaaaa cgcctagaac acctaagctc
      661 tgacttttgg gcttttgcgt ttttatgtta ggcattgtct ggcaactgga ggaagtgtaa
      721 tgaccatgaa tggtcactgt gattgacagt atcttgctta aacttatctg aacaatggta
      781 gaactggctc atatttatcc cctctatgat tgtttatctt tttcaccata aaaagtatca
      841 ggggctactc agggctacat aaagatattt atctactttt actgattgat aagtagcatc
      901 agtccatccg caggactggt ggtgccggca aaatgctgac ccaacacatg cgtatagatt
      961 tgcgtggtct taacatcgtt atgccctaag agttcttgca cagtgcgaat atcacgcccc
     1021 gcttgtaata gatgcgtagc aaacgagtga cgaaatgtat gacaagtgac acgcttgcta
     1081 acgatgcctg ctttttgtac ggctgccttc aatgcctttc gcgcaacgga gtcatgcaga
     1141 tgatggcggc ataatttgcc gttatacggg tggttgcaga gcgtgctgga gggaaagaca
     1201 aacatccacg ccgcttgtcg ataagcagaa gggtatttgt gatctaaagc aaaaggcagc
     1261 gatggcccta cgccttgtaa gttgtcgtct tgctgaataa gccgcgcttg ctcaatgagt
     1321 tattttattg ctgggattag gcgcgtgggc agtaggctgt ttctgctttt cccaccctta
     1381 ccgtcatgca cagtgatgca gccattatca aaatcaaaat ctttaacccg caaacgcaag
     1441 cattcattaa tgcgcaaacc tgcaccatac agcagcgtaa aaataacttg gttgcgagta
     1501 tccataacct gcaaaatgcg ttgcacttca tttgcagaga taacagaggg tagccgtcta
     1561 ggcttgcttg cagggatata atcaatatcg cccaacggct gttgtaaaaa cctgttgtac
     1621 aaaaaagcta gggcatttaa agcgattttc tgcgtgttta tggctacatg tctgctgttt
     1681 gctaagctgg ataaaaacag cctgacctct tcactgccca tggtctgagg atgacgtttt
     1741 ttgtgaaaca gaataaaacg cttaatccag tgcaggtaag ttttttcagt tttcagcgca
     1801 taaccttttt gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt agacataaaa
     1861 cgctccttgt cttgcaactg tctgcctata cagcctattc tagctgggat ttaaaaaagt
     1921 gcctgttttt tacgcctaga gatgcttgtt taccggtaga gttttaattt aatgctaaat
     1981 aaattaaaat gttatgagtt ctttgggtga gataatgtgc atcgtgcaag caggatagac
     2041 ggcatgcacg atttgtaata acagagtgtc ttgtattttt aaagaaagtc tatttaatac
     2101 aagtgattat attaattaac ggtaagcatc agcgggtgac aaaacgagca tgcttactaa
     2161 taaaatgtta acctctgagg aagaattgtg aaactatcac taatggtagc tatatcgaag
     2221 aatggagtta tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga acagctcctg
     2281 tttaaagcta ttacctataa ccaatggctg ttggttggac gcaagacttt tgaatcaatg
     2341 ggagcattac ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac atctgacaat
     2401 gagaacgtat tgatctttcc atcaattaaa gatgctttaa ccaacctaaa gaaaataacg
     2461 gatcatgtca ttgtttcagg tggtggggag atatacaaaa gcctgatcga tcaagtagat
     2521 acactacata tatctacaat agacatcgag ccggaaggtg atgtttactt tcctgaaatc
     2581 cccagcaatt ttaggccagt ttttacccaa gacttcgcct ctaacataaa ttatagttac
     2641 caaatctggc aaaagggtta acaagtggca gcaacggatt cgcaaacctg tcacgccttt
     2701 tgtaccaaaa gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc gtcatatgaa
     2761 gatttcggtg atccctgagc aggtggcgga aacattggat gctgagaacc atttcattgt
     2821 tcgtgaagtg ttcgatgtgc acctatccga ccaaggcttt gaactatcta ccagaagtgt
     2881 gagcccctac cggaaggatt acatctcgga tgatgactct gatgaagact ctgcttgcta
     2941 tggcgcattc atcgaccaag agcttgtcgg gaagattgaa ctcaactcaa catggaacga
     3001 tctagcctct atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca
     3061 cagtctcatc gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt
     3121 agagacacaa acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct
     3181 cggcggcatt gacctgttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat
     3241 gtactggtac tggttctcgg gagcacagga tgacgcctaa caattcattc aagccgacac
     3301 cgcttcgcgg cgcggcttaa ttcaggagtt aaacatcatg agggaagcgg tgatcgccga
     3361 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     3421 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     3481 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     3541 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     3601 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     3661 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3721 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3781 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3841 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3901 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3961 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     4021 gcaggcttat cttggacaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     4081 tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
     4141 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagagagc tggggaagac
     4201 tatgcgcgat ctgttgaagg tggttctaag cctcgtactt gcgatggcat cggggcaggc
     4261 acttgctgac ctgccaattg ttttagtgga tgaagctcgt cttccctatg actactcccc
     4321 atccaactac gacatttctc caagcaacta cgacaactcc ataagcaatt acgacaatag
     4381 tccatcaaat tacgacaact ctgagagcaa ctacgataat agttcatcca attacgacaa
     4441 tagtcgcaac ggaaatcgta ggcttatata tagcgcaaat gggtctcgca ctttcgccgg
     4501 ctactacgtc attgccaaca atgggacaac gaacttcttt tccacatctg gcaaaaggat
     4561 gttctacacc ccaaaagggg ggcgcggcgt ctatggcggc aaagatggga gcttctgcgg
     4621 ggcattggtc gtcataaatg gccaattttc gcttgccctg acagataacg gcctgaagat
     4681 catgtatcta agcaactagc ctgctctcta ataaaatgtt aggcctcaac atctagtcgc
     4741 aagctgaggg gaaccactag tgtcatacga acctccaaga gacggttaca caaacgggta
     4801 cattgttgat gtcatgtatg acaatcgccc aagtaagtat ccagctgtgt tcagaacgta
     4861 cgtccgaatt ccagactatg tcgaactaga taacgcggag gagctggccg acgcaatcat
     4921 tcgagaggtt gagttaaagg aggcatggct cacaag
//
LOCUS       DAJTXQ010000470         3817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000470 DAJTXQ010000000
VERSION     DAJTXQ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.guided.235"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..171
                     /locus_tag="OO515_004531"
     CDS             <1..171
                     /locus_tag="OO515_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCR6578248.1"
                     /translation="AHERTPQQERQKAAREAERGREAWTLGQGMKKPVAGCYGRLTRW
                     KGGGDVVYMALL"
     gene            complement(203..853)
                     /gene="tetR(A)"
                     /locus_tag="OO515_004532"
     CDS             complement(203..853)
                     /gene="tetR(A)"
                     /locus_tag="OO515_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6578249.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            959..2158
                     /gene="tet(A)"
                     /locus_tag="OO515_004533"
     CDS             959..2158
                     /gene="tet(A)"
                     /locus_tag="OO515_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6578250.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2125..2313)
                     /locus_tag="OO515_004534"
                     /pseudo
     CDS             complement(2125..2313)
                     /locus_tag="OO515_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2357..2746)
                     /locus_tag="OO515_004535"
     CDS             complement(2357..2746)
                     /locus_tag="OO515_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6578251.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2964..3404
                     /locus_tag="OO515_004536"
     CDS             2964..3404
                     /locus_tag="OO515_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6578252.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          806 a         1090 c         1080 g          841 t
ORIGIN      
        1 gcccacgagc ggacgccgca gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc
       61 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt
      121 ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt
      181 tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga
      241 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
      301 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
      361 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
      421 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
      481 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
      541 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
      601 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
      661 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
      721 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
      781 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
      841 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
      901 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
      961 gaaacccaac agacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
     1021 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
     1081 caccgcccac tatggcattc tgctggcgct gtatgcgttg atgcaatttg cctgcgcacc
     1141 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggccg gtcttgctcg tctcgctggc
     1201 cggcgctgct gtcgactacg ccatcatggc gacggcgcct ttcctttggg ttctctatat
     1261 cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg cttatattgc
     1321 cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg cctgtttcgg
     1381 gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct ccccccacgc
     1441 tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct gtttcctttt
     1501 gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca acccgctcgc
     1561 ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg tcttcttcat
     1621 catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg aggatcgctt
     1681 tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc tgcattcact
     1741 cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc gggcactcat
     1801 gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga cacggggatg
     1861 gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc cggcgctgca
     1921 agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag gctcactggc
     1981 ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga tctatgcggc
     2041 ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct acttgctctg
     2101 cctgccggcg ctgcgtcgcg ggctttggag aaattcttca aattcccgtt gcacatagcc
     2161 cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac
     2221 gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc
     2281 ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt
     2341 acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg
     2401 ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt
     2461 acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga
     2521 cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa
     2581 aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa
     2641 aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca
     2701 tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt
     2761 tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa
     2821 atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac
     2881 gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac
     2941 acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa
     3001 caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca
     3061 caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc
     3121 tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc
     3181 aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg
     3241 ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg
     3301 agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa
     3361 agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct
     3421 gatttaccat aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg
     3481 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt
     3541 ttttacggca tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac accattcagc
     3601 cggggtaagt cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt
     3661 tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca gcacgttcag
     3721 gaagggggtg atgtacaaat ggcacagagg atgaaagtga cgtatgagct gaaggaatga
     3781 tgtaaaagtg atgtataagt gatgtaaaag tgatgta
//
LOCUS       DAJTXQ010000471         3388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.guided.247, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000471 DAJTXQ010000000
VERSION     DAJTXQ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.guided.247"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            513..1328
                     /gene="sul2"
                     /locus_tag="OO515_004537"
     CDS             513..1328
                     /gene="sul2"
                     /locus_tag="OO515_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6578253.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1389..2192
                     /locus_tag="OO515_004538"
                     /note="aph(3'')-Ib"
     CDS             1389..2192
                     /locus_tag="OO515_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6578254.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2192..3028
                     /locus_tag="OO515_004539"
     CDS             2192..3028
                     /locus_tag="OO515_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6578255.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          728 a          933 c          949 g          778 t
ORIGIN      
        1 gtaacaggag tgcacgagaa gatagattaa gaggtcttga agtagaggcc tgactgcggc
       61 tacactgaaa gaacagtttt ggtaatggcg gtctcgtgaa ccctgttacc gggttaagca
      121 gttccccaac tgacttaacc ttcgaacaaa ccgcctcccc aggcggtttt ttcgtttaca
      181 gagcaagaga ttacgattca ttgaaaagga tctcaagaag atcctttgta gattactatc
      241 aattaatctt ccgaacatga caccagtcac tgttgattac ggttaaaccg ctccgtaaca
      301 ggtttccctc gtattggtga cttgcagcac caaagaagca aaaaagataa atatatctac
      361 ttattgagag atgatatatt tatttccaaa atgctacaga tacatcatca tgaaacggat
      421 caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc gtatgagact
      481 catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc attttcggca
      541 tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg ccagacgcag
      601 ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac ctcggtccgg
      661 catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatcgcgc
      721 cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt tatcaacccg
      781 cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc
      841 cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt
      901 cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca
      961 ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc
     1021 gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct
     1081 cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg
     1141 tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat gtcggggccg
     1201 cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc cgcacacacg
     1261 agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa accgcaagaa
     1321 ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga aaactgaagg
     1381 aacctccatt gaatcgaact aatatttttt ttggtgaatc gcattctgac tggttgcctg
     1441 tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat gccttcgcga
     1501 aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc
     1561 tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg gcaggaggaa caggagggtg
     1621 catgcttggt gataacggca attccgggag taccggcggc tgatctgtct ggagcggatt
     1681 tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc ctatcggttg
     1741 atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt
     1801 cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg ccgcagctcg
     1861 atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag cgcaccgata
     1921 tggttgtttg ccatggtgat ccctgcatgc cgaacttcat ggtggaccct aaaactcttc
     1981 aatgcacggg tctgatcgac cttgggcggc tcggaacagc agatcgctat gccgatttgg
     2041 cactcatgat tgctaacgcc gaagagaact gggcagcgcc agatgaagca gagcgcgcct
     2101 tcgctgtcct attcaatgta ttggggatcg aagcccccga ccgcgaacgc cttgccttct
     2161 atctgcgatt ggaccctctg acttggggtt gatgttcatg ccgcctgttt ttcctgctca
     2221 ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt tccagcctcg tttggaaagt
     2281 ttcattgcca gacgggactc ctgcaatcgt caagggattg aaacctatag aagacattgc
     2341 tgatgaactg cgcggggccg actatctggt atggcgcaat gggaggggag cagtccggtt
     2401 gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc ggggagcgaa tgctctctca
     2461 catcgttgcc gagcacggcg actaccaggc gaccgaaatt gcagcggaac taatggcgaa
     2521 gctgtatgcc gcatctgagg aacccctgcc ttctgccctt ctcccgatcc gggatcgctt
     2581 tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca ggttgtcaaa ctgactacgt
     2641 ccacgcggcg attatagccg atcaaatgat gagcaatgcc tcggaactgc gtgggctaca
     2701 tggcgatctg catcatgaaa acatcatgtt ctccagtcgc ggctggctgg tgatagatcc
     2761 cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat atgttctacg atccggctga
     2821 cagagacgac ctttgtctcg atcctagacg cattgcacag atggcggacg cattctctcg
     2881 tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg tacgcttatg ggtgcctttc
     2941 cgcagcttgg aacgcggatg gagaagagga gcaacgcgat ctagctatcg cggccgcgat
     3001 caagcaggtg cgacagacgt catactagat atcaagcgac ttctcctatc ccctgggaac
     3061 acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt ccgccccttc
     3121 ccgcaaacga cccctggaag ccccgtagcg acgcggagag gggcgagaca agccaagggc
     3181 gcaggctcga tgcgcagcac gacatagccg gttctcgcaa ggacgagaat ttccctgcgg
     3241 tgcccctcaa gtgtcaatga aagtttccaa cgcgagccat tcgcgagagc cttgagtcca
     3301 cgctagatct atctcatctg cgcaaggcag aacgtgaaga cggccgccct ggacctcgcc
     3361 cgcgagcgcc aggcgcacga ggccggcg
//
LOCUS       DAJTXQ010000472         3387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466655-rid15819243.guided.248, whole genome shotgun
            sequence.
ACCESSION   DAJTXQ010000472 DAJTXQ010000000
VERSION     DAJTXQ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466655
            Sequence Read Archive: ERR10074521
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477187.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477187.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,542
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 114 of 246
            Pseudo Genes (incomplete)         :: 62 of 246
            Pseudo Genes (internal stop)      :: 94 of 246
            Pseudo Genes (multiple problems)  :: 24 of 246
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466655-rid15819243.guided.248"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-08-08"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            513..1328
                     /gene="sul2"
                     /locus_tag="OO515_004540"
     CDS             513..1328
                     /gene="sul2"
                     /locus_tag="OO515_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6578256.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1433..2191
                     /locus_tag="OO515_004541"
     CDS             1433..2191
                     /locus_tag="OO515_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR6578257.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2191..3027
                     /locus_tag="OO515_004542"
     CDS             2191..3027
                     /locus_tag="OO515_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6578258.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          728 a          933 c          949 g          777 t
ORIGIN      
        1 gtaacaggag tgcacgagaa gatagattaa gaggtcttga agtagaggcc tgactgcggc
       61 tacactgaaa gaacagtttt ggtaatggcg gtctcgtgaa ccctgttacc gggttaagca
      121 gttccccaac tgacttaacc ttcgaacaaa ccgcctcccc aggcggtttt ttcgtttaca
      181 gagcaagaga ttacgattca ttgaaaagga tctcaagaag atcctttgta gattactatc
      241 aattaatctt ccgaacatga caccagtcac tgttgattac ggttaaaccg ctccgtaaca
      301 ggtttccctc gtattggtga cttgcagcac caaagaagca aaaaagataa atatatctac
      361 ttattgagag atgatatatt tatttccaaa atgctacaga tacatcatca tgaaacggat
      421 caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc gtatgagact
      481 catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc attttcggca
      541 tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg ccagacgcag
      601 ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac ctcggtccgg
      661 catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatcgcgc
      721 cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt tatcaacccg
      781 cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc
      841 cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt
      901 cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca
      961 ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc
     1021 gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct
     1081 cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg
     1141 tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat gtcggggccg
     1201 cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc cgcacacacg
     1261 agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa accgcaagaa
     1321 ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga aaactgaagg
     1381 aacctccatt gaatcgaact aatatttttt tggtgaatcg cattctgact ggttgcctgt
     1441 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1501 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     1561 caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc
     1621 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     1681 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     1741 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     1801 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     1861 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
     1921 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
     1981 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
     2041 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
     2101 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
     2161 tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat
     2221 tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt
     2281 tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct
     2341 gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg
     2401 ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac
     2461 atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag
     2521 ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt
     2581 gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc
     2641 cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat
     2701 ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc
     2761 gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac
     2821 agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt
     2881 gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc
     2941 gcagcttgga acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc
     3001 aagcaggtgc gacagacgtc atactagata tcaagcgact tctcctatcc cctgggaaca
     3061 catcaatctc accggagaat atcgctggcc aaagccttag cgtaggattc cgccccttcc
     3121 cgcaaacgac ccctggaagc cccgtagcga cgcggagagg ggcgagacaa gccaagggcg
     3181 caggctcgat gcgcagcacg acatagccgg ttctcgcaag gacgagaatt tccctgcggt
     3241 gcccctcaag tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc ttgagtccac
     3301 gctagatcta tctcatctgc gcaaggcaga acgtgaagac ggccgccctg gacctcgccc
     3361 gcgagcgcca ggcgcacgag gccggcg
//
